LOCUS       DASRIE010000001        16670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2606276, whole genome shotgun sequence.
ACCESSION   DASRIE010000001 DASRIE010000000
VERSION     DASRIE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606276"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFB53_00005"
     CDS             <1..198
                     /locus_tag="VFB53_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZN85140.1"
                     /translation="SAGRPASAAVSAIAGMPKSEFVARMNAFRAGTRPATVMHQIAKG
                     YDAREIELLAAWFAALPKEAR"
     gene            195..1457
                     /locus_tag="VFB53_00010"
     CDS             195..1457
                     /locus_tag="VFB53_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN85141.1"
                     /translation="MKTFDRRDFLKLGGAAAALSAAGCATTGAKARVVVIGGGFGGAT
                     AAKYIRLWDPAIEVVLVEREEAFTSCPISNLVLGGFTGMDTVRHGYGGLQRYGVQVVR
                     DEAVAVDPAKKTVRLRRGGDLAYDRLVVSPGIDFTYGDVQGYEAAMQSGRVLHAWKAG
                     AQTVALRRQLEDMRDGGVYILSIPTAPYRCPPGPYERASVVAAYFKAAKPRSKVLVLD
                     ANPDVTSKGGLFKAAWKDLYGGILEYRGNAKAVGVDAGGGTVRLEVEDVKGDVLNVVP
                     PQRAGDIAVQAGLVTHNNRWCDVDWRSMESKAAPGVHLLGDATLSAPGMPKSASMANN
                     HAKIAAAAVIEQLSGRAPQPLKIINTCYSFVSGSDGIRVSSVHQWDEAQKTLLAVKGA
                     GGVSAARSAAEGNYAWNWARTIWADSLG"
     gene            complement(1454..1903)
                     /gene="dut"
                     /locus_tag="VFB53_00015"
     CDS             complement(1454..1903)
                     /gene="dut"
                     /locus_tag="VFB53_00015"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZN85142.1"
                     /translation="MRRLEVRVLDPRIRDQLPQYATPGAAGLDLRACLDAPLALQPGD
                     SQLVPSGIAIHLGDAGLAAIVLPRSGLGAKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     RSAFTIAPLERIAQLVVVPVVQVAFEVVEEFAASARGAGGFGSTGKG"
     gene            complement(1890..3098)
                     /gene="coaBC"
                     /locus_tag="VFB53_00020"
     CDS             complement(1890..3098)
                     /gene="coaBC"
                     /locus_tag="VFB53_00020"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZN85143.1"
                     /translation="MTLKGKRLLLGITGGVAAYKAAQLARLLAKEGADLRVAMTEAAT
                     RFVTPVTLQALSGQPVWTDLWDPRVPDNMGHIELSRDRELIVVAPASADFLGKLAAGL
                     AGDLLSTLCLARRCPLMAAPAMNVEMWENPAMQRNLHALRGDGVFLAGPAAGDQACGE
                     VGMGRMLEPEEIVAEIEAFFQPKLLSGKRIVVTAGPTEEAIDPVRSITNASSGRMGYA
                     VARAAREAGARVTLVSGPVALAAPAGVERIDVKSAEQMFEAVKKAVAQSDVFIAVAAV
                     ADYRVKNRAAQKIKKGAPGLTLELAENPDILAHVAAMKNGPFCVGFAAESEKLAQNAR
                     AKRAKKGIPLLAANLAPAAIGAEDNEITLFDDAGEHPLGRGPKLQLARKLVAHLAGML
                     AGEKRARAKA"
     gene            3160..3834
                     /gene="radC"
                     /locus_tag="VFB53_00025"
     CDS             3160..3834
                     /gene="radC"
                     /locus_tag="VFB53_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZN85144.1"
                     /translation="MGIADWPLRERPRERLVAQGAAGLSDAELVAVCLRSGIRGKSAV
                     DLGRELIGAFGGIAGLLAADLAALLRVRGLGTAKAAQLAAILELARRSLREQLKSGAA
                     LTSPGAVRDYLRLALGGRPHEVFVCLYLDAQHRVTGAEELFRGSLTQTSVYPREVVKA
                     ALAANAAAVIFAHNHPSGAAQPSQADELLTRQLKEALALVEVKVLDHFIVAGSQTLSF
                     AERGLL"
     gene            3866..4114
                     /gene="rpmB"
                     /locus_tag="VFB53_00030"
     CDS             3866..4114
                     /gene="rpmB"
                     /locus_tag="VFB53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZN85145.1"
                     /translation="MSRVCQVTGKKPMKGNLVSHANNKTKRVFNPNLHYRRFWVETQN
                     RWVRLRVSTAGLRKIDKLGVEAVLAEIRARAKAEAKAA"
     gene            4117..4272
                     /gene="rpmG"
                     /locus_tag="VFB53_00035"
     CDS             4117..4272
                     /gene="rpmG"
                     /locus_tag="VFB53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZN85146.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKLEIRKYDPKARKHVL
                     YKETKLK"
     gene            complement(4336..5508)
                     /locus_tag="VFB53_00040"
     CDS             complement(4336..5508)
                     /locus_tag="VFB53_00040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN85147.1"
                     /translation="MIFSGWFDLPWWGLVLVALGLTHVTIASVTIFLHRHQAHRALDL
                     HPIASHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETAEDPHSPQVLGINSVLWGGVLL
                     YVKESHKPETMERYGHGTPDDWLEHHVYARFAILGLTLMGLANVLLFGIVPGMLILAT
                     QIVWIPFWAAGVINGIGHYWGYRSFDIVDASKNIVPWGILIGGEELHNNHHAYATSAK
                     LSAKWYEFDIGWMYIRILEALGLATVKKVAPTPRFEPAKTMCDYDTLHAVIANRYDVL
                     ARYATAVKRTWSDEIERLQHWSPRDAELLKPLKRALVRIGHGAETDRARVAEALKPSR
                     PLQVAVSMRGELLALWERSHASKEQLLRQLQDWCHRAEASGIVPLMDFSRRLRSYA"
     gene            complement(5662..6912)
                     /locus_tag="VFB53_00045"
     CDS             complement(5662..6912)
                     /locus_tag="VFB53_00045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HZN85148.1"
                     /translation="MIYRAQLARAEALLGELLAAAGPADAAVSRYFRAHRNLGQQDRA
                     FVAETVYAVLRRRRSLEAAAQSASPRDLAIAALVRVRGLSGRALEDVLREDEQALVAR
                     IRAAKTESFPRAVRADLPDWLWQRLDALHGEAEAMRVAQGLLNPAPLDLRVNLARLGR
                     DEALAQLRAGGIEAQPTPHSPAGLRLAAKPAINRHPLFAQGAIEVQDEGSQLLAYLLA
                     PRRGEMVADYCAGAGGKTLAIAMLMRGTGRIYAMDVSAKRLAELAPRAARAGVTSVHP
                     LVLSGEGDARARRLAGKLDRVLVDAPCSGFGTLRRNPDLKWRHGPQAIAELALKQAGI
                     LEAAARLLKPGGRLVYATCSILPEENEAVADAFASAHAEFAPLSCRELLAAQRIELDS
                     GERLRLWPHRHGCDGFFAAAFERR"
     gene            complement(6912..7598)
                     /locus_tag="VFB53_00050"
     CDS             complement(6912..7598)
                     /locus_tag="VFB53_00050"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZN85149.1"
                     /translation="MQRLLTAIALSLAAAAQAAPPARVEITYQLLHNGDAVAEVTHLL
                     EHDGRTYTLSERWAGRGLYAMLGGAQRTSRGRVTPGGLRPQEYLDERPGRNTASARFS
                     WREQTLTQQYRAEPQAQPLPPQAQDRLSFLYAPAFRAPAGKPLEYRVADGKGVAHYVF
                     DIAGRERLKLPWGEVDALRLVKREEGKDQRSTQIWLDPARSHLPLRVLVVQKDGTRVD
                     QVAARIAAAP"
     gene            complement(7567..8184)
                     /gene="purN"
                     /locus_tag="VFB53_00055"
     CDS             complement(7567..8184)
                     /gene="purN"
                     /locus_tag="VFB53_00055"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZN85150.1"
                     /translation="MSVVILISGRGSNMQAILEAGIPVAAVLSNRADAAGLAVAARRG
                     VATGVVEHRAYATREAFDAALAAAIDRHAPRLVALAGFLRVLTPRFVAQYAGRLMNIH
                     PSLLPEFPGLDTHARALAAGAQRHGCTVHFVTERLDGGPIIAQAAVPVLPGDSEAALA
                     ARVLAEEHRLYPQAIRWFLEGRLVIEGDRVRVKEAHAAPAHGDRA"
     gene            complement(8197..9075)
                     /locus_tag="VFB53_00060"
     CDS             complement(8197..9075)
                     /locus_tag="VFB53_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZN85151.1"
                     /translation="MKRRAFLLAAAGLAAHPAAAAPQRYAQGLLWRVSRPGVAPSHVF
                     GTLHAADARLAALPPPVLRAFDGSRALMLEYVADGYGRERFLEAAMYLDRRTLEEEIG
                     REDFERAWESLRPAGLSREFVNKLKPWGVLLNLRSSWRPAAVDTPDAQLHAMARGRRM
                     AVAQIEEVEEQVFTFDELPMASQVALLRHSLARREELAALAERTLEAYLARDLEALWR
                     AQEDVARRHPEIAAHHAALVKRVVLDRSVVMAFRMQRELRRGRAFVALGALHLYGERG
                     VLALLAEDGYAATRLY"
     gene            complement(9072..10466)
                     /gene="fumC"
                     /locus_tag="VFB53_00065"
     CDS             complement(9072..10466)
                     /gene="fumC"
                     /locus_tag="VFB53_00065"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZN85152.1"
                     /translation="MSATRLEKDSFGPIEVPAARLWGAQTERSRRYFRISGERMPAAV
                     IHALAQVKRSAARVNRALGLLAPDKAAAIERAADEVLAGQHDAEFPLVVWQTGSGTQS
                     NMNVNEVLANRASELLGGERGQKRLVHPNDDVNLGQSSNDVFPTAMHIAAARAVEHDL
                     LPALQRLQATLAAKAEAFAGIVKIGRTHLQDATPLTLGQEFSGYVEQLAQAEAAIELA
                     LPGLCELAIGGTAVGTGLNTHPEFGARVALDLSQATGLAFEPAPNKFAALAGHEPLVF
                     AHGALKALATALNKIANDVRWLASGPRSGLGELSIPENEPGSSIMPGKVNPTQAEALT
                     MLCAQVLGNDVAIGVGAAGGNFELNVYKPMLIHNYLQSVRLLADGCSSFEEHCARGIQ
                     PNEARIRELVERSLMLVTALAPHIGYDRAAEIAKKAHQEGSTLRAAALALGYVSAADY
                     ERWVQPGDMTKPSA"
     gene            complement(10499..11107)
                     /locus_tag="VFB53_00070"
     CDS             complement(10499..11107)
                     /locus_tag="VFB53_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85153.1"
                     /translation="MHTTTETLRREVARVIAARVTPVTPTLLVRRALPAAGLHRVGPW
                     TLLDHFGPLPSAPEPRPDTLTYLLEDAGAGMLHGLQFGARHLGTWRYAAESLAAFRAG
                     EAQLRVLCGELMGRRGPVPAPWPALVAHVALAGRGRAGLELPAGFELAAYVALGHARC
                     GVVEADAGKLMRFARDGAALEVANDLDERADVLLLGGAPIGA"
     gene            complement(11193..11576)
                     /gene="trxA"
                     /locus_tag="VFB53_00075"
     CDS             complement(11193..11576)
                     /gene="trxA"
                     /locus_tag="VFB53_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN85154.1"
                     /translation="MATVELNRGNFEKVVTESPMVIVDFWATWCGPCRGFAPIYEKAA
                     EQHPDVVFGKIDTDQEQELAGAFGIRSIPTLMVFREKVVVFQQAGALPASALEQVISQ
                     AKALDMAQVHKDIAEREAQGEQKQA"
     gene            11627..12472
                     /gene="gluQRS"
                     /locus_tag="VFB53_00080"
     CDS             11627..12472
                     /gene="gluQRS"
                     /locus_tag="VFB53_00080"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZN85155.1"
                     /translation="MAGYVGRFAPSPTGPLHFGSLVAALASYMEAKAAGGKWLLRMED
                     LDRPREQPGAADAILRALEALGFEWDGPVLVQSARLARYREVLADLARRGLAYPCGCT
                     RREIEDSALAIDGSRIYPGTCRHGLPPGKAARATRARTHRAPIGFADRFQGWVEQNVE
                     QEVGDFVLLRADGLVAYQLAVVVDDMDQGVTDVVRGADLLESTARQIHLQRLLGARTP
                     RYAHVPVALNAAGEKLSKQTGARPLELADPQAELARARRFLGQDEGGWDPARVPRLRA
                     ISAPE"
     gene            complement(12453..13349)
                     /locus_tag="VFB53_00085"
     CDS             complement(12453..13349)
                     /locus_tag="VFB53_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN85156.1"
                     /translation="MSPGQGEHLPRHLWWVLIGITLVWGFNWTAMKVAISEVAPFTFR
                     TFCLAAGSGVLFAVLRATGQPLAIPRAQWPRVLLIAFFSITCWNLLVVFGLRLIPSGR
                     AAILAYTMPAWAIPLSVWFLGERMNAKKLLGLALGMCGMALLLVQEFTSLAGAPLGAL
                     LVLGAAFTWAIGTVLQKKFPVQAPVAALTAWMMLAGGVPIYLGALLFDDFRALGGVGL
                     WPALAVAYNVLFSFAWAHWAWIKLATSVSVTTFSLAMLMTPVVGVFSGMLFLGERPGW
                     LEFAALLLVLGSLATVVIPARK"
     gene            complement(13346..14563)
                     /locus_tag="VFB53_00090"
     CDS             complement(13346..14563)
                     /locus_tag="VFB53_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN85157.1"
                     /translation="MSAPLDYKPDDPAFLADPFPLFRRMQDEDPCHWSPRLKAWVLTR
                     YEDVRRVCLDKEMSSDRLRPYFATLPPPEAQRIADIMRYLSLWMVFRDPPEHTRLRRL
                     TSKVFHVKSMHAMRARIEALVQRLFDRIADRREFDFIGDFAGPLPALVIMDMLGVPQE
                     ELASVKRLSDDMALFIGSSRMSPDKYDIAQAATREMAAFFRGMVAARRARPQADLLSE
                     LVQLEDEGDRLSDDELVATCILLLFAGHETTTNHIANGLLALLRFPGELARLRADPAL
                     APAAVEELLRYDGPNGAQVRIVREPQALHGKTLKAGERVFILLNAANRDPRAYPDPDR
                     VRIGRDGPPHLAFGFGLHICLGFPLARLEGQLALPAALSRWPGLELAGEPPAWLNSMV
                     FRGMTSMPVRVCA"
     gene            complement(14560..15441)
                     /locus_tag="VFB53_00095"
     CDS             complement(14560..15441)
                     /locus_tag="VFB53_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN85158.1"
                     /translation="MNAGADPIQRAAWVFPGLFVVLWSTGFIGAKLGLPYAEPATFLE
                     IRFLAVLAILLPLCWLLRAPWPQTRAEIWHMAFAGALIQGGYLGGVFLSLAQGMPAGV
                     SALITALQPILAAVLSGWLLGERTNARQWLGLALGIAGVALVVREKWVLEGVGFAATA
                     LSVLALASISLGAIWQKRHCARVDLRTGAAIQFASAMLVLSPVALFLETRQVTWSAEL
                     LVAIAWLVFALSLGAVFLLFWLIRHGAATKVASLMYLVPPCTALVAWPLFGETYTAFS
                     AAGMALAMLAVWLVSKG"
     gene            15636..16178
                     /locus_tag="VFB53_00100"
     CDS             15636..16178
                     /locus_tag="VFB53_00100"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZN85159.1"
                     /translation="MYVTPEQIQAAGKANVDTMLSFAQMHFAAIEKLAALNANAVKSA
                     FEDSLSNTRALFGAKDVQEFVGLQSTLAAPAIEKAIAYSKSVYEVATGTNAEISKVAE
                     QRVAEWNENFTALLDKAVKNAPAGSDVAVAGIKSMLAAANSAYDNFSKVTKQATDIAE
                     ANVAAATETVKGLAKAKKAA"
     gene            complement(16281..>16670)
                     /locus_tag="VFB53_00105"
     CDS             complement(16281..>16670)
                     /locus_tag="VFB53_00105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZN85160.1"
                     /translation="ATAALTLARNFLGDAFERTDVHIHVPAGATPKDGPSAGVAMFLA
                     LASLMQDKPIRPDVAMTGEISLRGLVLPIGGVKEKTLAALRAGIGTVMLPRRNEKDLE
                     DVPAEARAKLSFVFLDKVEDAVKTAFL"
BASE COUNT         2482 a         6003 c         5968 g         2217 t
ORIGIN      
        1 agcgccggac ggccggccag cgccgcggtg agcgcaatcg ccggcatgcc gaagagcgag
       61 ttcgtggccc gcatgaacgc ctttcgcgcc ggcacgcgcc cggcgacggt gatgcaccag
      121 atcgccaagg gctacgacgc gcgggaaatc gagctgctcg cggcgtggtt cgccgcgctg
      181 ccgaaggagg cccgatgaaa acgttcgacc ggcgcgactt cctcaagctc ggcggcgcgg
      241 ctgccgccct gtccgccgcg ggctgcgcca ccaccggcgc caaggcgcgc gtggtggtga
      301 tcggcggcgg cttcggcggc gcgaccgccg ccaagtacat ccgcctgtgg gacccggcca
      361 tcgaggtggt gctggtggag cgcgaagagg ccttcacctc ctgcccgatc tccaacctgg
      421 tgctcggcgg cttcaccggc atggacacgg tgcggcacgg ctatggcggg ctgcaaaggt
      481 acggcgtgca ggtggtgcgc gacgaggccg tcgcggtgga cccggcgaag aagaccgtgc
      541 ggctgcgccg cggcggcgat ctcgcctacg accgcctggt ggtgtcgccg ggcatcgatt
      601 tcacctacgg cgacgtgcag ggctacgagg cggcgatgca gtcgggccgc gtgctgcacg
      661 cctggaaggc gggcgcgcag accgtcgcgc tgcgccggca gctggaggac atgcgcgacg
      721 gcggcgtgta catcctgtcc atcccgacgg cgccctaccg ctgcccgccg gggccctacg
      781 agcgcgccag cgtggtggcc gcgtacttca aggccgccaa gccgcgctcc aaggtgctgg
      841 tgctcgacgc caacccggac gtcacctcga aaggcggcct gttcaaggcg gcctggaagg
      901 acctctacgg cggcatcctc gagtaccgcg gcaacgccaa ggcggtgggc gtggacgcgg
      961 gcggcggcac ggtgcggctc gaggtcgagg acgtgaaggg cgacgtgctg aacgtggtac
     1021 cgccgcagcg cgccggcgac atcgccgtgc aggccggcct ggtcacccac aacaaccgct
     1081 ggtgcgacgt cgactggcgc agcatggaat cgaaggcggc gccgggcgtg cacctgctcg
     1141 gcgacgccac gctgtcggcg ccgggcatgc ccaagtcggc cagcatggcc aacaaccacg
     1201 ccaagatcgc ggccgccgcg gtgatcgagc agctgagcgg ccgcgcgccg cagccgctca
     1261 agatcatcaa cacctgctac agcttcgtct ccggcagcga cggcatccgc gtctcctcgg
     1321 tgcaccagtg ggacgaggcg cagaagacgc tgctcgcggt gaagggcgcc ggcggcgtgt
     1381 cggcggcgcg cagcgcggcc gagggcaact acgcctggaa ctgggcgcgc accatctggg
     1441 ccgactcgct cggctagccc ttgccggtcg agccgaagcc gccggcgccg cgcgcgctgg
     1501 cggcgaactc ctccaccacc tcgaacgcca cctgcaccac cggcaccacc accagctgcg
     1561 cgatgcgctc cagcggcgcg atggtgaagg cgctgcggcc gcggttccac accgagacga
     1621 agatctgccc ctggtagtcg gagtcgatca ggccgacgag attgccgagc acgatgccgt
     1681 gcttggcgcc caggcccgag cgcggcagca cgatcgccgc aaggcccgcg tcgccgaggt
     1741 ggatggcgat tcccgagggc accagctggc tgtcgccggg ctgcagcgcg agcggcgcgt
     1801 ccaggcaggc gcgcaggtcg aggccggcgg cgcccggcgt ggcgtactgc ggcagctggt
     1861 cgcggatccg ggggtcgagg acccggactt caagccttcg cacgcgcgcg cttctctccc
     1921 gccagcatgc cggcgaggtg cgccacgagc ttgcgcgcca gctgcagctt gggcccgcgg
     1981 ccgaggggat gctcgccggc gtcgtcgaac agcgtgatct cgttgtcctc ggcgccgatc
     2041 gccgccggcg ccaggttggc ggcgagcagc gggatgccct tcttcgcgcg cttggccctg
     2101 gcgttctgcg ccagcttttc gctctcggcg gcgaaaccga cgcagaacgg gccgttcttc
     2161 atcgccgcca cgtgcgccag gatgtccggg ttctcggcga gctccagcgt caggccgggc
     2221 gcgcccttct tgatcttctg cgcggcgcgg ttcttcaccc ggtagtcggc gaccgccgcc
     2281 accgcgatga acacgtcgct ctgcgccacc gccttcttca ccgcctcgaa catctgctcg
     2341 gcgctcttca cgtcgatgcg ctcgaccccc gcgggcgcgg cgagcgccac cgggccgctc
     2401 accagcgtga ccctggcgcc cgcctcgcgc gcggcgcgcg ccaccgcgta gcccatcctg
     2461 ccggagctgg cattggtgat cgagcgcacc gggtcgatcg cttcctcggt cggcccggcg
     2521 gtcaccacga tgcgcttgcc ggagaggagc ttgggctgga agaaggcttc gatctcggcc
     2581 acgatctcct cgggctccag catgcgaccc atgccgacct cgccgcaggc ctggtcgccc
     2641 gccgccgggc cggcgaggaa tacgccgtcg ccgcgcagcg cgtgcaggtt gcgctgcatc
     2701 gccgggttct cccacatctc gacgttcatc gccggcgcgg ccatcagcgg gcagcggcgt
     2761 gcgaggcaca gcgtcgagag caggtcgccg gcgaggccgg cggccagctt gccgaggaaa
     2821 tccgcgctcg ccggggccac cacgatcagc tcgcggtcgc gcgacagctc gatgtgaccc
     2881 atgttgtccg gcacgcgcgg gtcccacagg tcggtccaca ccggctggcc gctgagcgcc
     2941 tgcaaggtca cgggggtgac gaagcgggtg gccgcctcgg tcatcgccac gcgcaggtcc
     3001 gcgccttcct tggcgaggag gcgggcgagc tgggccgcct tgtaggcggc gaccccgccg
     3061 gtgatgccca gcaacagcct tttgcccttc aaggtcatct tgcggtgggc tggagcgtta
     3121 aggggacagg gattctaggg gaattccggg agggccgcat tgggcatcgc cgactggccg
     3181 ctgcgggagc ggccgcgcga aaggctggtg gcgcagggcg ccgccgggct gtcggacgcc
     3241 gaactggtgg ccgtgtgcct gcgctcgggc atccgcggca agagcgcggt ggacctgggg
     3301 cgcgagctga tcggcgcctt cggcggcatc gccggcctgc tggccgcgga cctcgcggcg
     3361 ctgctgcggg tgcgcggcct ggggaccgcc aaggcggcgc agctcgccgc catcctcgag
     3421 ctcgcgcgcc gctccctgcg cgaacagctg aaatccggcg ccgccctcac ctcccccggc
     3481 gcggtgcgcg actacctgcg cctcgcgctc ggcggccggc cgcacgaagt gttcgtatgc
     3541 ctctacctgg acgcccagca ccgcgtcacc ggggccgagg agctgttccg cggcagtctc
     3601 acgcagacca gcgtctaccc gcgcgaagtg gtcaaggcgg cgctcgcggc caacgccgcc
     3661 gcggtgatct tcgcccacaa ccacccctcg ggcgcggccc agccctcgca ggccgacgag
     3721 ctgctcacgc gccagctgaa ggaggccctc gcgctggtcg aggtgaaggt gctcgaccac
     3781 ttcatcgtcg ccggcagcca gaccctctcg ttcgcggagc gcggcctgct ataatccgcg
     3841 cctttcgcga attccggagt ccgccatgtc ccgtgtctgc caggtcaccg gcaagaagcc
     3901 gatgaagggc aacctcgtct cgcacgccaa caacaagacc aagcgcgtgt tcaaccccaa
     3961 cctgcactac cgccgcttct gggtcgagac gcagaaccgc tgggtgcgcc tgcgggtgtc
     4021 caccgccggc ctgcgcaaga tcgacaagct gggcgtggaa gccgtgctcg ccgagatccg
     4081 cgccagggcc aaggccgaag cgaaggcggc ctgaccatgc gcgagaagat caagctcgaa
     4141 tccagcgccg gcaccggcca cttctacacc accaccaaga acaagcgcac cacgccggac
     4201 aagctggaga tcaggaagta cgatcccaag gcgcgcaagc acgtgctgta caaggaaacc
     4261 aagctgaagt agcggccggc gtcgccgcaa aagaaaaggc ccgctcgcgc gggcctttct
     4321 cattcccggg gccgctcagg cgtagctgcg caggcgccgg gagaaatcca tcagcggcac
     4381 gatgccgctc gcctctgcgc ggtggcacca gtcctgcagc tggcgcagca gctgctcctt
     4441 ggaggcgtgc gagcgctccc acagggcgag cagctcgccg cgcatgctta ccgccacctg
     4501 cagcggcctg gagggcttga gcgcctcggc gacgcgcgcg cggtcggttt ccgcgccgtg
     4561 gccgatgcgc accagcgcgc gcttcagggg cttgagcagc tcggcgtcgc gcggcgacca
     4621 gtgctgcagg cgctcgatct cgtcgctcca ggtgcgcttg accgccgtgg cgtagcgcgc
     4681 cagcacgtcg tagcggttgg cgatcacggc gtgcagcgtg tcgtagtcgc acatggtctt
     4741 cgccggctcg aagcgcggcg tcggcgccac cttcttcacc gtcgcgaggc ccagcgcctc
     4801 gaggatgcgg atgtacatcc agccgatgtc gaactcgtac cacttggccg agagcttcgc
     4861 cgaggtggcg taggcgtggt ggttgttgtg cagctcctcg ccgccgatca ggatgcccca
     4921 gggcacgatg ttcttgctgg cgtcgacgat gtcgaagctg cggtagcccc agtagtggcc
     4981 gatgccgttg atcacgccgg ccgcccagaa cgggatccag acgatctgcg tcgccaggat
     5041 cagcatcccc ggcacgatgc cgaacagcag cacgttggcg aggcccatga gcgtcaggcc
     5101 aaggatggcg aagcgcgcgt acacatggtg ctcgagccag tcgtcggggg tgccgtggcc
     5161 gtagcgctcc atggtctcgg gcttgtgcga ctccttcacg tagagcagca cgccgcccca
     5221 cagcaccgag ttgatgccca gcacctgcgg gctgtgcggg tcttcggcgg tctcgcactt
     5281 ggcgtggtgc ttgcggtgga tcgccgccca ttccttggtg accatgccgg tggtcagcca
     5341 gagccagaag cggaaaaaat gcgacgcgat cgggtgcagg tccagcgcgc ggtgcgcctg
     5401 gtgccggtgc aggaagatgg tcaccgaggc gatggtgacg tgcgtcagcc cgagggccac
     5461 gagcaccagg ccccaccagg gcaggtcgaa ccatccggaa aaaatcatca gttctttctg
     5521 atagttatga gcgaacagcg gctccgattg tacgcgattg gaccccccgc gcaaccaggg
     5581 aattcccgat tcttccgcaa ctgcgccgcg ccagccctaa ggtggcggcg cagaagggga
     5641 attcaaggtt tgcagcgcaa ctcagcgccg ctcgaacgcg gcggcgaaga aaccgtcgca
     5701 gccgtggcgg tggggccaca ggcgcagccg ctcgccgctg tcgagctcga tgcgctgcgc
     5761 ggcgagcagc tcgcggcagg acagcggggc gaattcggca tgcgccgacg cgaaagcgtc
     5821 cgccaccgcc tcgttctcct ccggcaggat gctgcaggtg gcgtacacca gccgcccgcc
     5881 cggcttcagc aggcgcgccg ccgcctcgag gatgcccgcc tgcttcagcg cgagctcggc
     5941 gatcgcctgc gggccgtggc gccacttgag gtcgggattg cgccgcagcg tgccgaagcc
     6001 gctgcagggc gcgtccacca gcacgcggtc gagcttgccc gcgaggcgcc tggcgcgcgc
     6061 gtcgccctcg cccgagagca ccagcggatg cacgctggtc acgccggcgc gcgccgcgcg
     6121 cggcgcgagc tccgcgagcc gcttcgccga cacgtccatg gcgtagatgc gcccggtgcc
     6181 gcgcatcagc atggcgatcg ccagggtctt gccgcccgcg cccgcgcagt agtcggccac
     6241 catttcgccg cggcgcggcg cgagcaggta ggcgagcagc tggctgccct cgtcctgcac
     6301 ctcgatcgcg ccttgcgcga acagcgggtg ccggttgatc gccggcttgg cggcgaggcg
     6361 cagccccgcc ggcgagtgcg gcgtgggctg cgcctcgatg ccgcccgcgc gcagctgcgc
     6421 cagcgcctcg tcgcggccga gcctggcgag gttcacgcgc aggtcgaggg gcgccggatt
     6481 gagcaggccc tgcgcgacgc gcatcgcctc cgcctcgccg tgcagcgcgt ccaggcgctg
     6541 ccacagccag tcgggcaggt cggcgcgcac cgcgcgcggg aaggactcgg tcttcgccgc
     6601 ccggatgcgc gccaccagcg cctgctcgtc ctcgcgcagc acgtcctcca gcgcgcggcc
     6661 ggagagcccg cgcacgcgca ccagcgcggc gatcgccagg tcgcgcggcg atgccgactg
     6721 cgccgcggcc tcgagcgagc gccggcggcg cagcaccgcg tacacggtct cggcgacgaa
     6781 ggcgcggtcc tgctggccga ggttgcggtg cgcgcggaag tagcgcgaca cggccgcatc
     6841 ggcggggccc gcggcggcga gcagctcgcc gagcagcgcc tcggcgcgcg ccagctgcgc
     6901 gcggtagatc atcacggcgc ggccgcgatg cgcgccgcca cctggtcgac gcgcgtgccg
     6961 tccttctgca ccaccagcac gcgcagcggg aggtgggaac gcgccgggtc gagccagatc
     7021 tgcgtcgagc gctggtcctt gccctcctcg cgcttcacca gccgcagcgc gtcgacctcg
     7081 ccccagggca gcttcagccg ctcgcgcccg gcgatgtcga acacgtagtg cgccacgccc
     7141 ttgccgtcgg ccacgcggta ctcgagcggc ttgccggcgg gggcgcgaaa ggcgggggcg
     7201 tacaggaagc tcaggcggtc ctgggcctgc ggcggcaggg gctgcgcctg cggctcggcg
     7261 cggtactgct gggtgagggt ctgctccctc caggaaaagc gcgcgctcgc ggtgttgcgg
     7321 cccgggcgct cgtccaggta ttcctgcggc cgcagcccgc ccggcgtgac gcgcccgcgg
     7381 ctggtgcgct gcgcgccgcc gagcatcgcg tacaggccgc gccccgccca gcgctcgctc
     7441 aaggtgtagg tcctgccgtc gtgctcgagc agatgcgtga cctcggcgac cgcgtcgccg
     7501 ttgtgcagca gctggtaggt gatctccacg cgcgccggcg gcgcggcctg ggccgccgcc
     7561 gcgaggctaa gcgcgatcgc cgtgagcagg cgctgcatgc gcttccttaa cgcgcacccg
     7621 gtcgccctcg atgaccagcc ggccctcgag gaaccagcgg atggcctgcg gatagaggcg
     7681 gtgctcctct gcgagcacgc gcgcggcgag cgcggcctcg ctgtccccgg gcagcaccgg
     7741 caccgcggcc tgcgcgatga tcggaccgcc gtcgagccgc tcggtgacga aatgcaccgt
     7801 gcagccgtgg cgctgcgcgc ccgcggcaag cgcgcgcgca tgggtgtcca gccccggaaa
     7861 ctcgggcagc agcgacgggt ggatgttcat caggcgcccg gcgtactggg cgacgaagcg
     7921 cggcgtcagc acgcgcagga agccggcgag cgcgaccagg cgcggcgcgt ggcggtcgat
     7981 ggccgcggcg agcgccgcgt cgaacgcctc gcgcgtcgcg taggcgcggt gctcgaccac
     8041 gccggtggcc acgccgcggc gcgccgcgac ggcgaggccg gcggcgtccg cgcggttgct
     8101 gagcaccgcg gccaccggga tgccggcttc gaggatcgcc tgcatgttgc tgccgcggcc
     8161 cgagatgagg atgacgaccg acatgaggcg cgcagtctaa tacagtcgcg tggcggcgta
     8221 gccgtcctcg gcgagcagcg ccagcacgcc gcgctcgccg tacaggtgca gcgcgcccag
     8281 cgccacgaag gcgcgcccgc gccgcagctc gcgctgcatg cggaacgcca tcaccacgct
     8341 gcggtcgagc accacgcgct tcaccagcgc cgcgtggtgc gccgcgatct ccggatggcg
     8401 gcgcgcgacg tcctcctgcg cgcgccacag ggcctccagg tcgcgcgcca ggtaggcctc
     8461 gagcgtgcgc tcggcgagcg ccgccagctc ctcgcgccgc gccaggctgt ggcgcagcag
     8521 cgccacctgc gaggccatcg gcagctcgtc gaaggtgaac acctgctcct cgacctcctc
     8581 gatctgcgcc accgccatgc gccggccgcg cgccatggcg tgcagctgcg cgtcgggcgt
     8641 gtccaccgcc gcgggccgcc aggaactgcg caggttgagc agcacgcccc agggcttcag
     8701 cttgttgacg aattcgcgcg acaggcccgc gggccggagg ctctcccagg cgcgctcgaa
     8761 atcctcgcgc ccgatctcct cctccagcgt gcgccggtcg aggtacatcg ccgcctcgag
     8821 gaagcgctcc ctgccgtagc cgtcggccac gtactccagc atcagcgcgc gcgagccgtc
     8881 gaaggcacgc agcaccggcg gcggcagcgc ggccaggcgc gcgtccgcgg cgtgcagggt
     8941 gccgaagacg tggctgggcg cgacgccggg ccggctgacg cgccacagca ggccctgcgc
     9001 gtagcgctgc ggcgcggcgg cggccggatg ggcggcaagc ccggcggccg ccagcaggaa
     9061 cgcgcggcgc ttcacgccga cggcttggtc atgtcgccgg gctgcaccca gcgctcgtag
     9121 tccgccgcgc tgacatagcc cagggcgagc gccgccgccc gcagcgtgct gccctcctgg
     9181 tgcgccttct tcgcgatctc ggcggcgcgg tcgtagccga tgtgcggcgc gagcgcggtc
     9241 accagcatca gcgagcgctc caccagttcg cggatgcgcg cctcgttcgg ctggatgccg
     9301 cgcgcgcaat gctcctcgaa ggacgagcag ccgtcggcca gcaggcgcac gctctgcagg
     9361 tagttgtgga tcagcatcgg cttgtacacg ttgagctcga agttgccccc ggcggcgccc
     9421 acgccgatcg ccacgtcgtt gcccagcacc tgcgcgcaca gcatggtgag cgcctcggcc
     9481 tgcgtcggat tcaccttgcc cggcatgatc gagctgcccg gctcgttctc ggggatcgac
     9541 agctcgccca ggcccgagcg cggaccggag gcgagccagc gcacgtcgtt ggcgatcttg
     9601 ttgagcgccg tggcgagcgc cttcagcgcg ccgtgcgcga acaccagcgg ctcgtggccg
     9661 gcgagcgccg cgaacttgtt gggcgccggc tcgaaggcga ggccggtggc ctgcgacaga
     9721 tccagcgcca cccgcgcgcc gaactccggg tgcgtgttca ggccggtgcc caccgcggtg
     9781 ccgccgatcg ccagctcgca cagcccgggg agcgccagct cgatggcggc ctcggcctgc
     9841 gccagctgct cgacgtagcc ggagaactcc tggccgaggg tgagcggcgt ggcgtcctgc
     9901 aggtgcgtgc gcccgatctt gacgatgccg gcgaaggcct ccgccttggc ggcgagggtg
     9961 gcctgcaggc gctgcagcgc gggcagcagg tcgtgctcca cggcgcgcgc ggccgcgatg
    10021 tgcatcgccg tggggaacac gtcgttcgag gactgcccca ggttgacgtc gtcgttcggg
    10081 tgcaccaggc gcttctggcc gcgttcgccg cccagcagct cggaggcgcg attggcgagc
    10141 acttcgttca cgttcatgtt gctctgcgtg cccgagccgg tctgccacac caccagcggg
    10201 aattcggcgt cgtgctgccc ggcgagcacc tcgtcggcgg cgcgctcgat ggccgcggcc
    10261 ttgtccggcg cgagcaggcc gagcgcgcgg ttgacgcgcg ccgcggagcg cttcacctgc
    10321 gccagggcgt ggatcacggc ggccggcatg cgctcgccgg agatgcggaa gtagcggcgg
    10381 ctgcgctcgg tctgcgcgcc ccagaggcgc gcggcgggta cctcgatcgg gccgaacgaa
    10441 tccttctcga ggcgcgtggc gctcacatca aggccctcga ctgcggggag ggcccagtct
    10501 acgccccgat cggcgcgccg cccagcaaca gcacgtccgc gcgctcgtcg aggtcgttcg
    10561 ccacctcgag ggccgcgccg tcgcgcgcga agcgcatcag cttgccggcg tcggcctcga
    10621 ccacgccgca gcgtgcgtgg ccgagcgcca cgtaggcggc gagctcgaag cccgcgggca
    10681 gctccagccc ggcgcggccg cgcccggcga gcgccacgtg cgcgaccagc gccggccagg
    10741 gcgccggcac ggggccgcgc cggcccatca gctcgccgca cagcacgcgc agctgcgctt
    10801 cgccggcgcg gaatgcggcc agcgactccg cggcgtagcg ccaggtgccg aggtggcgcg
    10861 cgccgaactg cagcccgtgc agcatgccgg cgccggcatc ctcgagcagg taggtcagcg
    10921 tgtcggggcg cggctcgggc gccgagggca gcgggccgaa atggtcgagc agcgtccagg
    10981 gcccgacgcg gtgcagcccc gcggccggca gcgcacggcg caccagcagc gtgggcgtga
    11041 ccggggtgac ccgcgcggcg atgacgcgcg ctacctcgcg ccgcagggtt tcagtggtgg
    11101 tgtgcatggg gccgggagtt cacgggtcag accattggag tgcaggcccg ggcacaagtt
    11161 tccgccggtt cgcgcccggc agggcggtct gctcaggcct gcttctgctc gccctgcgcc
    11221 tcgcgctccg cgatgtcctt gtgcacctgc gccatgtcga gggccttggc ctgcgagatc
    11281 acctgctcca gggcggaggc cggcagcgcg cccgcctgct ggaacacgac caccttctcg
    11341 cggaacacca tcagcgtcgg gatggagcgg atgccgaagg cgcccgccag ctcctgctcc
    11401 tggtcggtgt cgatcttgcc gaacaccacg tccgggtgct gctcggcggc cttctcgtag
    11461 atcggcgcga agccgcggca gggcccgcac caggtggccc agaaatccac gatcaccatc
    11521 gggctttcgg tgaccacctt ctcgaaattt ccccggttca gctcgaccgt cgccatgctg
    11581 gcctttcgcg aaaaacgcgc attgtacgcg gtgtacccta gcccccatgg ctggatacgt
    11641 cgggcgtttc gccccgtcgc cgacagggcc tttgcacttc ggctcgctcg tcgcggcgct
    11701 cgccagttac atggaggcga aggccgccgg gggcaagtgg ctgctgcgca tggaggacct
    11761 ggaccggccg cgcgagcagc ccggggcggc ggatgcgatc ctgcgcgccc tcgaggcgct
    11821 cggcttcgag tgggacgggc cggtgctcgt ccagagcgcg cgcctcgcgc gctaccgcga
    11881 ggtgctggcg gacctggcgc ggcgcgggct cgcctacccc tgcggctgca cgcgccggga
    11941 aatcgaggat tcggcgctcg ccatcgacgg ctcgcgcatc tacccgggca cctgccgcca
    12001 cggcctgccc cccggcaagg cggcgcgcgc cacgcgggcg cgcacgcacc gcgcgcccat
    12061 cggcttcgcg gaccgcttcc agggctgggt cgagcagaac gtcgagcagg aggtcggcga
    12121 cttcgtcctg ctgcgcgccg acggcctggt cgcctaccag ctcgcggtgg tggtggacga
    12181 catggaccag ggcgtcaccg acgtggtgcg cggcgccgac ctgctcgagt ccaccgcgcg
    12241 ccagatccac ctgcagcggc tgctgggcgc gcgcacgccg cgctacgcgc atgtgcccgt
    12301 cgcgctgaac gcggcggggg agaagctctc caagcagacc ggcgcgcggc cgctcgagct
    12361 cgccgacccg caggcggaac tggcgcgcgc gcgacgcttc ctcgggcagg acgagggcgg
    12421 ctgggacccc gcgcgcgtgc cgcgcctgcg ggctatttcc gcgccggaat gaccaccgtg
    12481 gcgagcgacc ccagcaccag cagcagcgcc gcgaactcca gccaccccgg gcgctcgccg
    12541 aggaacagca tgcccgagaa cacgcccacc accggcgtca tcagcatggc gagcgagaag
    12601 gtggtcaccg acaccgaggt ggcgagcttg atccaggccc agtgcgccca ggcgaaggaa
    12661 aagagcacgt tgtaggccac cgccagcgcc ggccacaggc ccacgccgcc gagcgcccgg
    12721 aaatcgtcga acagcagcgc gccgaggtag atcggcaccc cgccggcgag catcatccag
    12781 gcggtgagcg cggccaccgg cgcctgcacg gggaacttct tttgcagcac cgtgccgatg
    12841 gcccaggtga acgccgcccc cagcaccagc agcgcgccga gcggcgcgcc ggcgaggctg
    12901 gtgaactcct ggaccaggag cagcgccatg ccgcacatgc ccagcgccag gccgaggagc
    12961 ttcttcgcgt tcatgcgctc gccgaggaac cacaccgaga gcgggatcgc ccaggccggc
    13021 atggtgtagg cgaggatcgc ggcgcggccc gaggggatga ggcgcagccc gaagaccacc
    13081 agcaggttcc agcaggtgat ggagaagaag gcgatcagca gcacgcgtgg ccactgcgcg
    13141 cgcgggatgg cgagcggctg gccggtggcg cgcagcacgg cgaacagcac gccggagccc
    13201 gcggcgaggc agaaggtgcg gaaggtgaag ggcgccacct ccgagatcgc caccttcatc
    13261 gccgtccagt tgaagcccca caccagcgtg atcccgatca gcacccacca caggtggcgc
    13321 ggcaggtgct cgccctgccc ggggctcaag cgcacacccg caccggcatc gaggtcatgc
    13381 cgcggaacac catcgagttg agccacgccg ggggctcccc ggcgagctcg aggcccggcc
    13441 agcgcgagag cgccgcgggc agcgccagct ggccctccag gcgggcgagc ggaaagccga
    13501 ggcagatgtg caggccgaag ccgaaggcga ggtgcggcgg cccgtcgcgc ccgatgcgca
    13561 cgcggtccgg gtcgggatag gcgcgcgggt cgcggttggc ggcgttcagc aggatgaaca
    13621 cgcgctcgcc cgccttgagg gtcttgccgt gcagcgcctg cggctcgcgc acgatgcgca
    13681 cctgcgcccc gttcgggccg tcgtagcgca gcagctcttc caccgccgcc ggcgcgagcg
    13741 cggggtcggc gcgcagcctt gccagctccc cgggaaagcg cagcagcgcc agcagcccgt
    13801 tggcgatgtg gttggtggtg gtctcgtgcc cggcgaagag cagcaggatg caggtggcca
    13861 ccagctcgtc gtcgctcagg cggtcgccct cgtcctccag ctgcaccagc tcggagagca
    13921 ggtcggcctg cggccgcgcg cgccgcgcgg cgaccatgcc gcggaagaac gcggccatct
    13981 cgcgcgtggc ggcctgcgcg atgtcgtact tgtcggggga catgcgcgag ctgccgataa
    14041 agagcgccat gtcgtccgag agccgcttca cgctggcaag ctcctcctgc ggcacgccca
    14101 gcatgtccat gatcaccagc gccggcagcg gcccggcgaa gtcgccgatg aagtcgaatt
    14161 cgcgccggtc ggcgatccgg tcgaacagcc gttgcaccag cgcctcgatg cgcgcgcgca
    14221 tggcgtgcat cgacttgacg tggaacacct tgctcgtcag gcggcgcagc cgcgtgtgct
    14281 cgggcgggtc gcggaacacc atccagaggg acaggtagcg catgatgtcg gcgatgcgct
    14341 gcgcctcggg cggcggcagg gtggcgaaat aggggcgcag gcggtccgag gacatctcct
    14401 tgtccaggca cacgcggcgc acgtcctcgt agcgcgtcag cacccaggcc ttgaggcgcg
    14461 ggctccagtg gcaggggtcc tcgtcctgca tcctgcggaa cagcgggaag gggtcggcga
    14521 ggaaggccgg gtcgtcgggc ttgtagtcga ggggcgcgct catcctttgc tcaccagcca
    14581 caccgcgagc atggcgagcg ccatgccggc ggcggagaac gcagtatagg tctcgccgaa
    14641 gagcggccag gcgaccagcg ccgtgcaggg cggcaccagg tacatcaggc tcgccacctt
    14701 ggtcgcggcg ccgtggcgga tgagccagaa gaggaggaac accgccccga gcgagagcgc
    14761 gaacaccagc caggcgatgg cgacgaggag ctccgcgctc caggtcacct ggcgcgtctc
    14821 caggaagagg gccacggggg acagcaccag catggcagag gcgaactgga tcgccgcgcc
    14881 ggtgcgcagg tcgacgcgcg cgcaatggcg cttctgccag atcgcgccca ggctgatgct
    14941 cgccagcgcg agcaccgaga gcgcggtggc ggcgaatccg acgccctcca gcacccattt
    15001 ctcgcgcacc accagcgcca cgcctgcgat ccccagcgcg agcccgagcc actggcgcgc
    15061 gttggtgcgc tcgcccagca gccagccgga gagcaccgcg gcgaggatgg gctgcagcgc
    15121 ggtgatcagc gccgacaccc cggccggcat gccctgggcg agcgagagga acaccccgcc
    15181 gaggtatccc ccctggatca gggcgccggc gaaggccatg tgccagatct cggcccgcgt
    15241 ctgcggccag ggagcgcgca gcagccagca cagggggagc aggatggcga ggacggccag
    15301 gaagcgaatt tcgaggaagg tggccggctc cgcgtaagga agtcctaact tcgcccctat
    15361 gaacccggtg ctccacagga ccacgaacag gcccgggaac acccaggccg cgcgctggat
    15421 ggggtcggcg ccggcgttca tgggccggca ttgtcgccgg gccaggcaat ccgcagggcg
    15481 ccgtttcaac taaaaggaaa tcagtgggtt atggtgcatt gcaaaaaaca tttgacttcc
    15541 ccgggcaaag gcctaaacta cgccttgctg cgctgcacca ttcagcgccc aaccgaagca
    15601 ccaaccgaat tttccacact tcaaggagcc tgaccatgta cgtgaccccc gagcaaatcc
    15661 aggcggccgg caaagccaac gtcgacacga tgctttcctt cgcccagatg catttcgcgg
    15721 cgatcgagaa gctcgcggcg ctgaacgcca acgccgtcaa gtccgctttc gaagacagcc
    15781 tgagcaacac ccgcgccctg ttcggcgcca aggacgtgca ggagttcgtc ggcctgcaga
    15841 gcaccctcgc cgcgcccgcc atcgagaagg ccatcgccta ctcgaagagc gtgtacgaag
    15901 tcgccaccgg caccaatgcc gagatctcca aggtcgccga gcagcgcgtc gccgagtgga
    15961 acgagaactt caccgccctg ctggacaagg ccgtgaagaa cgcgcccgcc ggctccgacg
    16021 tcgccgtcgc cggcatcaag tcgatgctcg ccgccgccaa ctcggcctac gacaacttca
    16081 gcaaggtcac caagcaggcg accgacatcg ccgaggccaa cgtggccgcc gcgaccgaga
    16141 ccgtgaaagg cctcgccaaa gcaaaaaaag ccgcctgagc gccaaaccgg tccggcgctc
    16201 gccggcccga tcaagcgcca agcgcgcagt caagaagaag aagtaaggaa agcccgccgt
    16261 ccggcgggct tttttttcgc ctacaggaac gcggtcttca ccgcgtcttc caccttgtcg
    16321 aggaacacga agctgagctt cgcgcgcgcc tcggccggca cgtcctcgag gtccttctcg
    16381 ttgcgcctgg gcagcatcac ggtgccgatg ccggcgcgca gcgcggcgag ggtcttttcc
    16441 ttcaccccgc cgatcggcag caccaggccg cgcagcgaga tctcgcccgt catcgccacg
    16501 tccgggcgga tcggcttgtc ctgcatcagc gaggccaggg cgaggaacat cgcgactccc
    16561 gcgctcggcc cgtctttcgg tgtcgccccg gccggcacgt ggatgtgcac gtcggtcctt
    16621 tcgaacgcat cgccgaggaa gttcctcgcg agcgtcagcg ctgcggtggc
//
LOCUS       DASRIE010000002         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1306356, whole genome shotgun sequence.
ACCESSION   DASRIE010000002 DASRIE010000000
VERSION     DASRIE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306356"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFB53_00110"
     CDS             complement(<1..1020)
                     /locus_tag="VFB53_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZN85161.1"
                     /translation="MRVLFATPECAPLVKVGGLGDVSAALPAALRALGVEVRVLLPGY
                     PEVLQKAAGLAELERFSELGFDCRLLGGGDLLVLDCPALYQRSGSPYQDPGGQDWPDN
                     ALRFGVLSRVAARLPGYDVLHCNDWQTALAPLLSRTPALLTIHNLAFQGNFERAWLDR
                     LGIGQEYFSIDRLEFHGRASFLKAGLVYAKALTTVSPTYAREIQTAEFGCGMEGLLRE
                     RRGALTGILNGIDTAEWNPAGDPHLAQRYDAQSLDKKALNKQALQEKLNLELRPEIPL
                     IGFVGRITHQKGVDLIAGAINELAALPAQFALLGRGERDLEGALSAAAGRHPGQVAMA
                     SAFDEP"
     gene            complement(1127..1444)
                     /locus_tag="VFB53_00115"
     CDS             complement(1127..1444)
                     /locus_tag="VFB53_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85162.1"
                     /translation="MKPEVADKLGDADDFYTLTKAVLALCEPYGAVHAFRLVHNRGAA
                     RVACLIEMESPKQQTVLAKALGARQLNGAACLDIPVRKDFGAPIKVAVLPQPAYEARA
                     AAR"
     gene            complement(1517..>2231)
                     /locus_tag="VFB53_00120"
     CDS             complement(1517..>2231)
                     /locus_tag="VFB53_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZN85163.1"
                     /translation="LARLSRGELFAIMAGGMASIAGTVLFLYGSILGRVMPDAVAHLL
                     IASILSAPAALVIAFLMVPPGNTQGEVLALKSDAAGSMDALTRGTLEGAQLLLNIVAM
                     LVVFVALVALVNLVIAPYSLQGALGWLLAPLAWLAGVSWAEAREAGALLGTKTVINEL
                     VAYLDLANSKGLSERSRILLTYALCGFANFGSLGIMIGGLGTMAPERRAEVVDLGIKS
                     IVAGTLATCLTAAAVALIL"
BASE COUNT          362 a          764 c          777 g          328 t
ORIGIN      
        1 cggctcgtcg aacgcgctcg ccatcgcgac ctgcccggga tggcggccgg cggcggctga
       61 cagcgcgccc tcgaggtcgc gctcgcccct gccgagcagg gcgaattgcg ccggcagcgc
      121 ggcgagctcg ttgatcgcgc cggcgatcag gtcgacgccc ttctggtgcg tgatgcggcc
      181 gacgaagccg atcagcggaa tttcgggccg cagctccagg ttgagcttct cctgcagcgc
      241 ctgcttgttc agcgccttct tatccagcga ctgcgcgtcg tagcgctgcg ccaggtgcgg
      301 atcgccggcc gggttccatt ccgcggtgtc gatgccgttc aggatgccgg tgagcgcgcc
      361 gcgccgctcg cgcagaagcc cttccatgcc gcagccgaat tccgcggtct ggatctcgcg
      421 cgcgtaggtc gggctcaccg tggtgagcgc cttggcgtag accaggccgg ctttcaggaa
      481 cgaggcgcgg ccgtggaatt cgagccggtc gatcgagaaa tattcctggc cgattcccag
      541 ccggtcgagc cacgcgcgct cgaaattgcc ctggaacgcg aggttgtgga tcgtgagcag
      601 cgccggcgtc ctcgagagca gcggcgcgag cgcggtctgc cagtcgttgc aatggagcac
      661 gtcgtagccc ggcaggcgcg ccgcgacgcg cgacagcacc ccgaagcgca gcgcattgtc
      721 cggccagtcc tggccgccgg gatcctggta ggggcttccg ctgcgctggt acagcgccgg
      781 gcagtccagc accagcagat ccccgccgcc cagcagccgg caatcgaagc cgagctcgct
      841 gaagcgctcg agctcggcga ggcccgccgc cttctgcagc acttccgggt agccgggcag
      901 cagcacgcgc acctcgacgc cgagcgcgcg cagcgccgcg ggcagcgccg cgctgacgtc
      961 gcccagcccg ccgaccttga cgagcggcgc gcattccggg gtcgcgaaca ggactcgcat
     1021 cagctcttct tatcccacaa aaaaaagacc ccggtagggg tgccggggtc gtgttgccta
     1081 catacgcacg aagggaggga agtgcgtatg tattggggga accgggtcag cgggcggcgg
     1141 ctctggcctc gtaagcgggc tgcggcagaa cggccacctt gatcggcgcg ccgaaatcct
     1201 tgcgcaccgg gatgtccagg caggcggcgc cgttcagctg gcgcgcgccg agcgccttgg
     1261 cgagcacggt ctgctgcttg ggcgactcca tctcgatcag gcacgcgacg cgcgcggcgc
     1321 cgcggttgtg caccaggcga aaagcatgca ccgcgccgta gggctcgcac agcgcgagca
     1381 ccgccttggt cagggtgtag aaatcgtcgg cgtcgccgag cttgtcggct acttcaggct
     1441 tcattgcaga tcgatgctat tgagcgggcc cccggccgcc tatcccaaaa ctcatgaacc
     1501 ttatgtagtg gcgccactac agaatcagcg ccaccgccgc cgcggtcagg caggtggcga
     1561 gcgtgccggc gacgatcgac tttatcccaa gatccacgac ttcggcgcgg cgctcgggcg
     1621 ccatggtgcc gaggccgccg atcatgatgc cgaggctgcc gaagttggcg aagccgcaga
     1681 gggcgtaggt gagcaggatg cggctccttt ccgagagccc tttggaattg gccaggtcca
     1741 ggtaggccac cagctcgttg atcaccgtct tggtgccgag caaggccccc gcctcgcgcg
     1801 cttccgccca cgacactccc gcgagccagg cgagcggcgc gagcagccag ccgagcgcgc
     1861 cctgcaggct gtagggggcg atgaccaggt tgacgagcgc caccagcgcc acgaacacca
     1921 ccagcatggc gacgatgttg agcaggagct gcgcgccctc gagcgtgccg cgcgtgagcg
     1981 cgtccatgct gccggcggca tcggacttga gcgccagcac ctccccttgg gtgtttccgg
     2041 gaggaaccat gaggaacgcg atcaccagcg cggccggcgc cgagaggatc gacgcgatca
     2101 gcaggtgcgc caccgcgtcg ggcatcaccc ggccgagaat gctgccgtag aggaacagca
     2161 ccgtgccggc gatcgaagcc atgccgcccg ccatgatcgc gaacagctcg ccgcgcgaga
     2221 gccgcgcgag g
//
LOCUS       DASRIE010000003         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1046002, whole genome shotgun sequence.
ACCESSION   DASRIE010000003 DASRIE010000000
VERSION     DASRIE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046002"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VFB53_00125"
     CDS             <1..1360
                     /locus_tag="VFB53_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZN85164.1"
                     /translation="GAQPAWDRVTEIGVIEIDGFAVSSEWSTLVNPGASIPAPIQALT
                     GITNEMVAAAPSFRELAGALFERLAGRVFVAHNARFDYGFLRHEFERAGLKFMAKTLC
                     TVRLSRRLYAAHASHNLDSLIARHGIACRARHRALGDADAVWQFLRIAAQEHGDEILA
                     VAARQIARQPALPPHIDRAVIDAIPEAPGVYLFYGEGGAPLYVGKSVSLRSRVLSHFC
                     CGLRSQKATELSREVRRIDWQRTVGELGALLREAKLVKALQPAYNRQLRRPESLCGFV
                     FDMKRLRLAHPHEIDGETLPYVYGLFRSRRDALEALRGLADEHRLCLQALGFETPVRP
                     RGCWRFQLGKCEGACAGRENIHVHHGRVAQALARLKAIDWPHPGPLGVVERDRNREAT
                     EVHVVDRWCYLGAARSDAELAELVGSARRPPFDYDEYRILARHLAKKSVRTLRLTGCT
                     AS"
     gene            1345..2337
                     /locus_tag="VFB53_00130"
     CDS             1345..2337
                     /locus_tag="VFB53_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85165.1"
                     /translation="MHCELIVPGLFAGAAGARVAALELLLARGRGSGAQSRPLEAWLQ
                     DAFGLHGRPLAAGALTLLAAGEDPGEASWARADPVHLRLMRERLIVAPSAAFAIAREE
                     ADALAEALNRHFEGLLLLKVIDAHRWCAQLQDDMAFDASSPLEAAGRDADLARTAGEA
                     GRRWGQLLNEAQMLLHAHPVNQARESRGEPMVNSLWLWGTGSVPRGVKGPWQSVSAAE
                     PIALGLARLAGARMRSLPPSAGEWLEQRPVEGQHLVVLDGLRAPLALGQEAEYRECIG
                     ALEKHWFAPLLAALRAGRVGMITAHVPEAGASFETIRGDLRRFWRRRRALERYA"
     gene            2382..>2704
                     /locus_tag="VFB53_00135"
     CDS             2382..>2704
                     /locus_tag="VFB53_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZN85166.1"
                     /translation="MEAGVHPVLARIYAARRIRSALELKYETAGLIAPKFLKSIEAAA
                     RLLADAIQAGKRMLIVADYDADGATACAVGLRALRMFGAQVDYLVPDRFKLGYGLSPE
                     LVELAA"
BASE COUNT          363 a          972 c          983 g          386 t
ORIGIN      
        1 cggcgcgcag cccgcctggg accgcgtgac cgagatcggg gtcatcgaga tcgacggctt
       61 cgcggtcagc tcggaatggt cgaccctggt caatcccggc gcgtccatac cggcgccgat
      121 ccaggcgctc accggcatca cgaacgagat ggtggcggcg gcgccgagct tccgcgagct
      181 cgccggcgcc ctgttcgagc gcctggccgg gcgcgtgttc gtggcgcaca acgcgcgctt
      241 cgactacggc ttcctgcgcc acgagttcga gcgcgccggg ctgaagttca tggcgaagac
      301 gttgtgcacg gtgcggcttt cgcgccgcct ctacgccgct cacgcgagcc ataacctcga
      361 cagcctgatc gcgcgtcacg gcatcgcctg ccgcgcgcgg caccgcgccc tcggcgacgc
      421 cgacgcggtg tggcagttcc tgcgcatcgc ggcgcaggag cacggcgacg agatcctcgc
      481 ggtcgccgca aggcagatcg cgaggcagcc tgcgctgccc ccgcacatcg accgcgccgt
      541 gatcgacgcc atccccgaag ctccgggcgt ctacctcttt tacggcgagg gcggcgcgcc
      601 gctctatgtc ggcaagagcg tctcgctgcg ctccagggtc ctgtcgcact tctgctgcgg
      661 cctgcgctcg cagaaggcga ccgagctctc gcgcgaggtg cgccgcatcg actggcagcg
      721 caccgtgggc gagctgggcg cgctgctgcg cgaagcgaag ctcgtcaagg cgttgcagcc
      781 cgcctacaac cgccagctgc ggcgtccgga aagcctttgc ggcttcgtct tcgacatgaa
      841 gcggctgcgc ctcgcgcacc cgcacgagat cgacggcgaa accctgccgt acgtgtacgg
      901 cctgttccgc tcccggcgcg acgccctcga ggccctgcgc ggcctcgccg acgagcaccg
      961 cctgtgcctg caggcgctgg ggttcgagac gccggtgcgc cccaggggat gctggcgctt
     1021 ccagctcggc aaatgcgaag gcgcctgcgc cggcagggaa aacattcacg tgcaccacgg
     1081 acgcgtggcg caggcgctgg cgcgtctcaa ggcgatcgac tggccgcacc ccgggccgct
     1141 gggggtcgtc gagcgcgacc gcaaccgcga agccacggag gtgcacgtgg tcgaccgctg
     1201 gtgctacctc ggagccgcgc gctcggatgc cgagcttgcc gagctcgtcg gctccgcacg
     1261 gcggccgccg ttcgattacg atgagtacag gatcctggcg cggcacctgg caaagaaaag
     1321 cgtgcgaacc ttacggctga ccggatgcac tgcgagctga tcgttccggg cctgttcgcc
     1381 ggagcggccg gcgcgcgggt tgccgccctc gagctgctgc tcgcccgcgg gcgcggctcc
     1441 ggcgcgcaat cgcggccgct cgaagcctgg ctgcaggacg ctttcggtct gcacggccgg
     1501 ccgcttgcgg ccggagcgct cacgctgctc gcggccggcg aggacccggg cgaggccagc
     1561 tgggcgcgcg ccgacccggt gcatctgcgg ctgatgcgcg agcggctgat cgtagcgccg
     1621 agcgcggcgt tcgccatcgc gcgggaggag gccgatgcgc tcgccgaggc cctgaatcgc
     1681 catttcgagg gcctgctctt gctgaaggtg atcgacgcgc accgctggtg cgcacagctc
     1741 caggacgaca tggcgttcga cgcctcctcg ccgctcgagg cagccgggcg cgacgccgat
     1801 ctcgcgcgca ccgcaggcga agccggcagg cgctggggcc agctgctcaa cgaggcgcag
     1861 atgctgctgc atgcgcatcc ggtaaaccag gctcgggagt cgcggggcga gccgatggtg
     1921 aacagtctct ggctctgggg caccgggagc gtcccgcgcg gcgtcaaggg gccctggcag
     1981 tccgtgagcg ccgccgagcc gatcgcgctc gggctcgcgc gcctggcggg cgcgcgtatg
     2041 cgctcccttc ccccttcggc aggcgaatgg ctcgagcaga ggcccgtgga gggccagcac
     2101 ctcgtggtgc tcgacgggct gcgcgcgccg ctcgcgctgg gccaggaggc ggagtaccgc
     2161 gagtgcatcg gcgcgctgga aaagcactgg ttcgcgcccc tgctcgccgc gctgcgcgcc
     2221 ggccgcgtcg gcatgatcac ggcgcacgtc cccgaggccg gcgcctcctt cgagaccatc
     2281 cgcggcgacc tgcgccggtt ctggcgccgg cggcgcgcgc tggagcgcta cgcgtgaaga
     2341 tcgtcgagcg cccgtactcg gagaccgatc gccgcaggct gatggaggca ggcgtgcacc
     2401 cggtgctcgc ccgcatctac gccgcccgcc gcatccgctc cgcgctggag ctgaagtacg
     2461 agacggcggg gctgatcgcg ccgaagtttc tgaaaagcat cgaggcggcg gcgcggcttc
     2521 tcgccgacgc gatccaggcg ggcaagcgca tgctgatcgt cgccgactac gacgccgacg
     2581 gcgcgaccgc gtgcgccgtg ggcctgcgcg cgctgcgcat gttcggcgcg caggtcgact
     2641 atctggtgcc ggaccggttc aagctcggct acggcctgtc gcccgagctg gtggagctcg
     2701 cggc
//
LOCUS       DASRIE010000004         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2348096, whole genome shotgun sequence.
ACCESSION   DASRIE010000004 DASRIE010000000
VERSION     DASRIE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348096"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFB53_00140"
     CDS             <1..438
                     /locus_tag="VFB53_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN85167.1"
                     /translation="VDRGDPARAEAEVRDLNEGVTCIRASFGDVDPAALLQVKPGTHS
                     FSGAPTHNSAIRSFALRFEHPVRRELLQQFISTLVDLRGPDLLRVKGFAPLESGMVAI
                     QGVRHVFDRMRPAEGGEPALVFITNGVEKAEVEALWRAMKELS"
     gene            592..4770
                     /locus_tag="VFB53_00145"
     CDS             592..4770
                     /locus_tag="VFB53_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN85168.1"
                     /translation="MLLIEDDPIMVEIVGSYLGRISFTQCALHSTPTLTEGLALLARL
                     EIDLVISDLHLPDSAGEATINALVSAVGCPVIAITSDLHPALRAATLSCGAYDFLHKS
                     DLSEAALSRLVRLAAMQARTFRSLRKSESRLRAIMNNEPQCVKLLDSEGRLIEMNPAG
                     LRMIEADSMDAMRGQCVYGLVVDEHRAAFRDLTRRVARGEQGSLEFEMTGLRGGRRWL
                     ETRAVPLRDDVTAKQLVLCITRDITERRQAEAALRESEARFRALTELSSDWYWEQDAE
                     LRFVRTAGASPARGGISAEVHVGLRRWELPRTEIVGQSWDEHRKVLDARQPFHELLLR
                     RTSEKGDVRYVSVDGRPIFDAGGSFTGYCGVAKDVTGRIASELALRDSEERFRHLTQL
                     SSDFYWESDARHRMTRMAHGSGTAPLGSEGPQLGKTRWQVPSTRPDDAGWAAHRAVLD
                     ARLPFRDFEVARMSAQGEERHFAVSGEPVFDAAGTFVGYRGTGREITARKREEAILAL
                     EHAVTRCLADAHTAATGLRSVLRTICESQGWPLGRYFEADEAAGLLRFSEAWGKPLLK
                     VEAMIDRSRAMVYARGQGLSGTVWQSGKPLWVRDVSKDRRTTGATRGTLQGGAFVFPI
                     TSGGRTIGVMSFSSADERHPDERLLRAIGVIGSQVGQFLMRKKAEHEERRRAEDLGRF
                     RAALDASADPVLLVDARTGAYVDFNEAACHTYGYSRDELLGMSVDQIRVDRSREQLLA
                     DYGRLCADPDSDQMEIAACRRKDGSVLPIEFTRRLVQTGDGPIIVATARDLTERLRAE
                     ARQAAVLKYQERVARFGQLALAQRQPAELIERAVQAVLEALGAEAVAYVEPEPGAAAL
                     VVRALVGVADANTQPATAPCPPGSPLGDAMHTGARAVVDGAGLPFPWAQELRSTVLLP
                     VRGDNGVRGMVCAGFKDPNAFAAEELNFAEAAASVLSAALQRIESENRLAYLAQFDPL
                     TGLANRTLLADRFSQMIAQARRHGTPLAVLFIDLDSFKTVNDTLGHAGGDELLKEVAL
                     RLQATVREGDTVARISGDEFAIVLADMAQLEDAALVAQKVIDGLAATVDVSGKEVFVT
                     ASVGIAVFPSDGADADTLIDAADAAMYRAKESGRNTFEFFTADISQRSRARAHMSAEL
                     RRALEREEFALVYQPKIDLATREATGAEALLRWKHPARGLVSPAEFIPVLEETGLIVP
                     VGDWVLRRACADLKAWQAAGLSVGPVAVNLSARQFRRGDLDSHIKALVAGVGVQPDLI
                     ELEITESQLMHDPDHAIRMMRALRDAGMRIAIDDFGTGYSSLAYLHRFPVSALKIDRS
                     FVKDMTKGKDGATIVRTIIELAHSLAFTVVAEGVETEEQANALGLLRCEYAQGFLFAK
                     PMPAEDYAKLLPKR"
     gene            4781..>5194
                     /locus_tag="VFB53_00150"
     CDS             4781..>5194
                     /locus_tag="VFB53_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN85169.1"
                     /translation="MKTLLLALALAWTASASAQSDYPNKPVRLVVSFAAGGISDVLAR
                     ALAIPLSRQLGQQVIVDNKPGAGTSIAGDYMAKAAPDGYTIWLQDITTHAINVNLYSK
                     LPYDSVKDFTFIAMVASTPLMLVVHPSTPANSVKEL"
BASE COUNT          820 a         1755 c         1827 g          792 t
ORIGIN      
        1 gtcgatcgcg gcgacccggc cagggccgag gccgaggtgc gggacctcaa cgaaggtgtc
       61 acctgcatcc gcgccagctt cggcgacgtg gatccggccg cgcttctcca ggtgaaaccc
      121 gggacgcatt ccttttccgg cgcgcccacg cacaacagcg cgatccgcag cttcgccttg
      181 cgtttcgagc atccggtgcg gcgcgagctg ctgcagcagt tcatctccac gctggtggac
      241 ctgcgcggcc ccgatctgct gcgcgtgaaa ggattcgcac ccctggaatc cggcatggtc
      301 gcgatccagg gggtgcgcca cgtcttcgac cgcatgcggc cggccgaggg cggggagccg
      361 gccctggttt tcatcaccaa cggggtggaa aaggccgaag tggaagcgct gtggagagcc
      421 atgaaagagc tctcctgaca agctacttgc ggtcttttgc gcatttcact gtatttgtaa
      481 ggtttttact ctagcgccaa ggggcaaggc cgggcatcct gtgagcccgg aactatgctg
      541 ccgcgccccg attttcccaa agcgctcggc ctgagcggtg aaccgctccg gttgctgttg
      601 atcgaggacg acccgatcat ggtggagatc gtcggctcgt atctcgggcg catctccttc
      661 acgcagtgcg cgcttcacag cacgcccacg ctgaccgaag gcctggcgct gctcgcgcgc
      721 ctggagatcg atctcgtcat ctccgacctg catctgccgg attccgcggg cgaggccacg
      781 atcaacgcgc tggtcagcgc ggtgggatgt ccggtgatcg cgatcacctc ggacctgcac
      841 cctgcgctgc gcgccgcaac gctgagctgc ggcgcctacg acttcctgca caagagcgac
      901 ctgtcggaag ccgcgctgtc gcggctggtg cgcctcgcgg cgatgcaggc gcggaccttc
      961 cgttcgctgc gcaagagcga gagccgcctg cgcgcgatca tgaacaacga gccgcaatgc
     1021 gtgaagctgc tcgacagcga aggccggctg atcgagatga acccggccgg gctgcgcatg
     1081 atcgaggccg acagcatgga cgcgatgcgc ggccaatgcg tgtacggcct ggtggtagac
     1141 gagcaccgcg cggcgtttcg cgacctgacc cggcgcgtgg cgcgcggcga gcagggttcg
     1201 ctcgaattcg agatgaccgg cctgcgcggc gggcggcgat ggctggaaac gcgcgcggtg
     1261 ccgctgcgcg acgacgtcac cgccaagcag ctggtgctct gcatcacgcg ggacatcacg
     1321 gagcgccggc aggccgaagc ggcgctgcgc gagagcgagg cgcgcttccg cgcgctgacc
     1381 gagctctcgt ccgactggta ctgggagcag gatgcggaat tgcgcttcgt gcgcaccgcc
     1441 ggcgcgagcc cggcgcgtgg cggcatcagc gccgaagtcc acgtgggatt gcgccgctgg
     1501 gagctgccga ggacggaaat cgtcggccag tcgtgggacg agcaccgtaa agtactcgac
     1561 gcgcgccagc cgttccacga gctgctgttg cgccgtacct ccgagaaggg ggacgtgcgc
     1621 tacgtcagcg tggacgggcg gccgattttc gacgccggcg gcagtttcac cggctattgc
     1681 ggcgtcgcga aggacgtcac cggccgcatc gcgtccgagc tggcgctgcg cgatagcgag
     1741 gagcgcttcc gccacctcac ccagctttcc agcgacttct actgggaaag cgacgcgcgg
     1801 caccgcatga cccgtatggc gcacggctca ggcaccgcgc cattgggtag cgaaggaccg
     1861 cagctcggca agacgcgctg gcaggtacct tccacgcggc ccgacgacgc cggctgggcc
     1921 gcgcaccgag ccgtgctgga tgcgcgcctt ccgtttcgcg acttcgaggt cgcgcgcatg
     1981 agcgcgcaag gcgaagaaag gcatttcgcg gtcagcggcg agccggtatt cgatgccgcg
     2041 ggcaccttcg tcggctaccg cggtaccggc cgcgagatca ctgcgcgcaa gcgcgaggag
     2101 gcgatcctcg cgctggagca cgcagtcacg cgctgcctgg cggacgccca caccgcggcg
     2161 accggcctga gatcggtgct gcgcaccatc tgcgaatcgc agggctggcc gctcggccgc
     2221 tatttcgagg cggacgaggc cgccgggttg ctgcggttca gcgaggcctg gggcaagccg
     2281 ctgctgaagg tggaggcgat gatcgaccgc tcgcgtgcga tggtgtacgc gcgcggccag
     2341 ggcctgtcgg gtacggtgtg gcaatcgggc aagccgctct gggtgcgcga cgtgagcaag
     2401 gaccggcgca ccaccggcgc cacgcgcggc accttgcaag gcggcgcctt cgtattcccg
     2461 atcacgtcgg gcggtcgcac gatcggcgtg atgagcttct ccagcgcgga tgagcgccat
     2521 cccgacgagc gcctgctgcg cgcaatcggc gtgatcggca gccaggtcgg ccagttcctg
     2581 atgcgcaaga aggcggagca cgaggagcgg cgccgcgcgg aggacctggg gcgtttccgc
     2641 gccgcgctcg acgcctccgc cgacccggtg ctgctggtgg acgcgcgcac cggcgcctat
     2701 gtcgatttca acgaggcggc gtgccatacc tacggctatt cgcgcgacga gctgctcggc
     2761 atgtcggtgg accagatccg ggtcgaccgc tcgcgcgagc agctgctcgc cgactatggg
     2821 cgcctgtgcg ccgatcccga cagcgaccag atggaaatcg ccgcctgccg gcgcaaggac
     2881 ggctcggtac tcccgatcga attcacccgc cgcctggtgc agacgggcga tgggccgatc
     2941 atcgtcgcga cggcgcgcga cctcaccgaa cgcctgagag ccgaggcgcg tcaggccgcg
     3001 gtactcaagt accaggagcg cgtggcgcgc tttggccagc ttgcgctggc gcagcgccag
     3061 ccggccgagc tgatcgagcg cgcagtgcag gcggttctgg aggcgctcgg cgccgaagcg
     3121 gtggcttatg tcgagcccga gccgggcgcg gctgcgctcg tggtgcgggc gctggtcggc
     3181 gtcgctgacg cgaatacaca gccggccacg gcgccctgtc cgccgggatc gccccttggc
     3241 gatgcgatgc acacgggagc gcgcgcggtg gtcgacgggg ccgggctgcc gttcccctgg
     3301 gctcaggagt tgcgcagcac cgtgctgctg ccggtacgcg gcgacaacgg cgtgcgcggc
     3361 atggtgtgcg ccggcttcaa ggacccgaac gcgtttgccg ccgaggaact gaacttcgcc
     3421 gaggcggcag cgagcgtgct gtcggccgcg ctgcagcgga tcgagagcga gaaccggctc
     3481 gcgtatctcg cgcagttcga cccgctcacc ggcctggcca accgcacgct gctcgccgac
     3541 cggttttcgc agatgatcgc gcaggccagg cgccacggca cgccgctcgc ggtgctgttc
     3601 atcgacctcg acagcttcaa gacggtcaac gacacgctcg gccacgccgg cggcgacgag
     3661 ctgctgaagg aggtggcgtt gcggctgcag gccacggtgc gcgagggcga cacggtggcg
     3721 cgcatcagcg gcgacgagtt cgccatcgtg ctcgccgaca tggcgcagct cgaggatgcc
     3781 gcgctggtcg cgcagaaggt gatcgacggc ctggcggcga cggtcgatgt gagcggcaag
     3841 gaagtcttcg tgacggcgag cgtcggcatc gccgtgttcc cgagcgacgg cgcggatgcc
     3901 gacacgctga tcgacgccgc cgacgccgcg atgtaccgcg ccaaggaatc gggccgcaac
     3961 accttcgagt tcttcaccgc cgacatcagc cagcgctcgc gcgcgcgcgc ccacatgagc
     4021 gccgagctgc gccgggcgct ggagcgcgag gaattcgcgc tggtctacca gccgaaaatc
     4081 gaccttgcca cccgcgaggc gaccggggcc gaggcgctgc tgcgctggaa gcatccggcg
     4141 cgcggcctgg tatcgccggc cgagttcatc ccggtgctgg aagagaccgg gctcatcgtg
     4201 ccggtcggtg actgggtgct gcggcgcgcc tgcgcggacc tgaaggcgtg gcaggcggcc
     4261 gggctctccg tcgggccggt cgcggtgaac ctgtcggcgc gccagttccg gcgcggcgat
     4321 ctcgactcgc atatcaaggc cctggtcgcc ggcgtcggcg tgcagcccga cctgatcgag
     4381 ctcgaaatca ccgagagtca gctgatgcac gaccccgacc acgccatccg catgatgcgc
     4441 gcgctgcggg acgccggcat gcgcatcgcg atcgacgatt tcggcaccgg ctattccagc
     4501 ctcgcctacc tgcaccgctt tccggtgagc gcgctgaaga tcgaccgctc gttcgtcaag
     4561 gacatgacca agggcaagga tggcgccacc atcgtgcgca ccatcatcga gctggcgcac
     4621 tcgctcgcct tcaccgtggt cgccgagggg gtcgagaccg aggagcaggc gaatgccctc
     4681 gggctgctgc gctgcgagta cgcgcagggc tttctgttcg ccaagccgat gccggccgag
     4741 gactacgcga agctgttgcc gaagcgctag actcgtcggc atgaaaacac tcttgcttgc
     4801 cttggcgctg gcgtggaccg catcggcctc tgcccagtcc gactatccga acaagccggt
     4861 gcggctggtg gtcagcttcg ccgccggcgg aatctccgac gtgctcgctc gcgcgttggc
     4921 gatcccgctc agcagacagc tcggccagca ggtgatcgtg gacaacaagc ccggcgccgg
     4981 caccagcatc gccggcgact acatggcgaa ggcggcgccc gacggctaca ccatctggct
     5041 gcaggacatc accacccacg cgatcaacgt caacctttac agcaagctgc cgtacgactc
     5101 ggtcaaggac ttcaccttca tcgccatggt cgcctccacg ccgctcatgc tggtggtgca
     5161 tccatcgacg ccggccaaca gcgtgaagga gctg
//
LOCUS       DASRIE010000005         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2610526, whole genome shotgun sequence.
ACCESSION   DASRIE010000005 DASRIE010000000
VERSION     DASRIE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610526"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFB53_00155"
     CDS             <1..849
                     /locus_tag="VFB53_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85170.1"
                     /translation="RDLRPALTLRAARTKDPELRRDEASVSYRRWSDDRRFRLEAGLL
                     GARLSSSLGDWSPRGVFASAWAQALPLSPQIDASYYDADVSRGARLFGSVQVEPVRDR
                     LKLRAGRVDWGRTAFSAGATADELTARTVGAVAEAGGTAGTLRARLDVYDISDGNKVF
                     DGDAQLTPTWQPLPWGLRWFGGAYLRDADREDARYWSPSHTYGVAFAGVQRHWTFDNA
                     EVSASLRRGFALSSSAGDSWSGGVSGRVWLRPDLALGVEGWAVDAPRPGSYRMHQVGA
                     FLQRLL"
     gene            846..2177
                     /locus_tag="VFB53_00160"
     CDS             846..2177
                     /locus_tag="VFB53_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN85171.1"
                     /translation="MIRAATALAIAGLLGAAALWMHSLAGSPAFTHSFAALGPASKVI
                     VLVLLGVLLLLGCRHLVMLGLAAWDHCMRPLAPEAAPGSRLPFMSIIVPAFNEGPMIA
                     QVLESLRLVRYPRFEVIVVDDGSSDQTFLRALPFRRGQGVEFRVLTKSNGGKFDALNH
                     GIARARGEIVVCIDGDSQLHPDALRHCAAHFDDPRVGAVAGNVRVANRGTGWSLLQAL
                     EYVVGYGLAKRAQSLARAVTIVPGPLGAFRRSALAQVKGYEGDSFAEDFDLTIRMLGA
                     GWHIVYEPRAIVYTEAPERTLELLRQRYRWTRGSLQVLRKRAHWLRRPLRDPLRFAGI
                     AYVALECLALPALHVAAQFLFVTGGLWLGAHEVVVYWWLQLLVLECAVVAFCLAVENE
                     HPALVLLTPVFRAFYMLVLDMARLLATLEEFKGVAMGWDKVARIGRLGPAG"
     gene            2181..2369
                     /locus_tag="VFB53_00165"
     CDS             2181..2369
                     /locus_tag="VFB53_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85172.1"
                     /translation="MDTLFHFLSNLVLFLTLTTLVFACSAYGVLALRRRRPLARRRAH
                     EPRADSALLRRYRPQDKD"
     gene            2372..>3474
                     /locus_tag="VFB53_00170"
     CDS             2372..>3474
                     /locus_tag="VFB53_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN85173.1"
                     /translation="MPALLRALFLFLAGVCALPAGAETPVVLLRSDTSAAFFRAHGGD
                     YERLLKPWRELFSRHGIAAREHPAAELARAGKPGVLILASSVALSEAERKAIRERIAE
                     GWSVLGTWAVGVRDGKGQWAGYGFIDELFAAQVASELAPAKDESFLLPYGETPLTHAL
                     PAGKRVYLASASEPFLRVRAANVAARFGNYMREVTAPGALLGAAAFDERAGARRAFFG
                     FAETAWDSAQADVDAMMVGTLRWLQRRPIALKSAWPHPYQSALLLEMDTEDKFDNSLR
                     FAEQLERHDIRGTFYSVTSEAQKFPEVVKRLGARHEIAYHAEVHTGFAKVGPREQEAR
                     LALMVRQLAKLVPNASAATGFRAPLEQYDANTE"
BASE COUNT          450 a         1245 c         1230 g          549 t
ORIGIN      
        1 cgcgacctgc gtcccgcgct cacgctgcgc gccgcgcgga ccaaggaccc cgagctgcgc
       61 cgcgacgagg cgtccgtctc ctaccgacgc tggagcgacg accgccgctt ccgcctggag
      121 gcgggcctgc tcggggcgcg cttgagctcg tcgcttggcg actggtcgcc gcgcggcgtg
      181 tttgcctcgg cctgggcgca ggcgctgccg ctttcgccgc agatcgacgc ctcctattac
      241 gacgccgacg tgagccgcgg cgcgcgcctg ttcggcagcg tgcaggtcga gccggtcagg
      301 gaccggctca agctgcgcgc gggccgcgtc gactggggaa gaaccgcgtt cagcgccggc
      361 gcgaccgcgg acgaactcac cgcgcgcacc gtaggagcgg tggccgaggc cggcggcacc
      421 gccggaacgc tgcgcgcccg cctggacgtg tacgacattt ccgacggcaa caaggtgttc
      481 gatggcgacg cgcagctcac gccgacgtgg cagccgctgc cgtggggcct gcgctggttc
      541 ggcggcgcct acctgcgcga cgccgaccgg gaggatgcgc gctactggtc gccgagccac
      601 acctatggcg tggccttcgc cggcgtgcag cgtcactgga ccttcgacaa cgcggaggta
      661 tctgcctcgc tgcggcgcgg attcgcgctc agcagctcgg ccggcgacag ctggagcggc
      721 ggcgtcagcg gccgcgtgtg gctgcggccg gaccttgcgc tcggcgtcga aggatgggcg
      781 gtggacgcgc ccaggccggg gagctaccgc atgcatcagg tgggcgcatt cctgcagagg
      841 ttgctgtgat ccgcgcggcg acagccctcg ccatcgccgg actgctcggc gccgcggcgc
      901 tgtggatgca ctcgctcgcg ggtagtcccg ccttcaccca cagcttcgcc gcgctcggcc
      961 cggcgagcaa agtcatcgtg ctggtgctgc tcggcgtgct gctgctgctc ggctgccgcc
     1021 acctcgtcat gctgggtctc gccgcgtggg atcactgcat gcggccgctg gcgcccgagg
     1081 ctgcgcccgg ctcgcgcctg ccgttcatgt ccatcatcgt tcctgccttc aacgaaggtc
     1141 cgatgatcgc ccaggtgctc gaatccctgc gtctggtgcg ctatccgcgc ttcgaggtca
     1201 tcgtggtgga cgacggctcc tcggaccaga ccttcctgcg ggcgctgccc ttccggcgcg
     1261 gccagggcgt ggagttccgc gtcctcacca agtcgaacgg cggcaagttc gacgcgctga
     1321 atcacggcat cgcccgcgcg cgcggcgaaa tcgtggtctg catcgacggc gacagccagc
     1381 tgcacccgga cgcgctgcgc cactgcgcgg cgcacttcga cgatccgcgc gtcggcgcgg
     1441 tggccggcaa cgtgcgcgtc gccaatcgcg gcaccggctg gtcgctgctg caggcgctcg
     1501 agtacgtcgt cggctacggg cttgcgaagc gcgcccagag cctggcgcgc gccgtgacca
     1561 tcgtgcccgg accgctcggc gccttccgca ggagcgcgct ggcgcaggtc aagggctacg
     1621 aaggcgacag cttcgccgag gatttcgacc tgaccatccg gatgctcggc gcaggctggc
     1681 acatcgttta cgagccgcgc gccatcgttt acacggaggc gcccgagcgc acgctcgagc
     1741 tgctgcgcca gcgctaccgc tggacccggg gatcgctgca ggtcctgcgc aagcgcgcgc
     1801 actggctgcg caggccgctg cgcgatccgc tgcgctttgc gggcatcgcc tacgtggcgc
     1861 tcgaatgcct cgcgctgccg gcgctgcacg tggcggcgca gttcttgttc gtgaccggag
     1921 gtctgtggct cggcgcgcac gaggtcgtgg tgtactggtg gctgcagctc ctggtgctcg
     1981 agtgcgcggt ggtcgccttc tgcctcgcgg tcgagaacga acatcccgcc ctggtccttc
     2041 tcacgccggt gtttcgcgcg ttctacatgc tggtgctcga tatggcgcgc ctcctggcga
     2101 cgctggagga attcaagggc gtggccatgg gttgggacaa ggtcgcgcga atcggccgac
     2161 tcggacctgc gggctaagcc atggacactc tcttccactt cctttcgaac ctggtgctgt
     2221 tcctcaccct gaccacgctg gtgttcgcgt gcagcgctta cggcgtgctc gcgctgcgcc
     2281 ggcgccggcc gctcgcgcgc cggcgcgcgc acgagccgcg cgccgactcg gcgctgttgc
     2341 gccgctaccg gccgcaagac aaggattgat catgcccgcc ctcctccgcg ccctgttcct
     2401 tttcctcgcc ggcgtttgcg cgctgccggc cggcgccgaa acgccggtcg tgctcctgcg
     2461 cagcgacacg agcgccgcgt tcttccgcgc ccacggcggc gactatgagc gcctgctcaa
     2521 gccctggcgc gagctcttct cgcgccacgg catagcggcg cgcgagcacc cggcggccga
     2581 gctggccagg gcgggcaagc cgggcgtgct gattctcgcg tcgagcgtgg cgttgagcga
     2641 ggccgagcgc aaggcgattc gcgagcggat cgccgagggc tggagcgtgc tcggcacctg
     2701 ggccgtcggc gtgcgcgacg gcaaagggca gtgggcggga tacggcttca tcgacgagct
     2761 gttcgccgcg caggtggcga gcgagctggc ccccgccaag gacgaatcgt tcctgctgcc
     2821 ctacggcgaa accccgctca cgcacgcgct gccggccggc aagcgcgtct acctcgcgag
     2881 cgcgagcgag cccttcctcc gggtgcgtgc ggcgaacgtc gccgcccgct tcggcaacta
     2941 catgcgcgaa gtcaccgccc ccggggcgct gctcggcgcc gcggcgttcg acgagcgcgc
     3001 cggcgcgcgg cgcgcgttct tcggcttcgc cgagacggcc tgggacagcg cacaggccga
     3061 tgtcgatgcc atgatggtcg gcacgctgcg ctggctgcag cggcgcccga tcgcgctgaa
     3121 aagcgcctgg ccgcacccgt accagtccgc gctgctgctg gagatggaca ccgaagacaa
     3181 gttcgacaac agcctgcgct tcgccgagca gctcgagcgg cacgacatcc gcggcacgtt
     3241 ctattcggta acctcggagg cgcagaagtt tcccgaggtg gtgaagcgcc tcggcgcgcg
     3301 gcacgagatc gcctaccacg ccgaggttca caccggtttc gccaaggtcg gaccacggga
     3361 gcaggaggcg cgcctcgccc tgatggtgcg ccagctcgcc aagctggtgc cgaacgcttc
     3421 ggcggccacc ggctttcgcg cgccgctcga gcagtacgac gcgaataccg agat
//
LOCUS       DASRIE010000006         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1047702, whole genome shotgun sequence.
ACCESSION   DASRIE010000006 DASRIE010000000
VERSION     DASRIE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047702"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFB53_00175"
     CDS             complement(<1..354)
                     /locus_tag="VFB53_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HZN85174.1"
                     /translation="MTSTDLQAVIAHMGAAARAASARMAAASTAAKNAALVALARRLR
                     DDADALAAPNERDIAAARSAGLAPPMVERLKLTPKIIGTVAEGCEQIAAMPDPIGEIT
                     NLRRRPSGISVGQMRV"
     gene            complement(395..952)
                     /locus_tag="VFB53_00180"
     CDS             complement(395..952)
                     /locus_tag="VFB53_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZN85175.1"
                     /translation="MPRFARTVSCTAAILACNGLAQAAGFTLASPTIKPGARIAEAQV
                     FDGFGCTGQNVSPALKWSGAPKGTRSYALTVYDPDAPTGSGWWHWVVVNIPANVTELP
                     EGAGTGAGRLPAGALQVRTDFGKPGWGGPCPPQGDKPHRYVFTLHALKTEKLDLPADA
                     TAALAGFMINANKLRTATFTGTYGR"
     gene            complement(1143..2159)
                     /locus_tag="VFB53_00185"
     CDS             complement(1143..2159)
                     /locus_tag="VFB53_00185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZN85176.1"
                     /translation="MSAAPPSPAVVLAAEGLARHYTVSLGFLRPKATVRALDGVSFTL
                     ERGRTLAVVGESGCGKSTLARQVTMIEPPTAGTLRIGGLDVAHADTAARRALRRQVQM
                     VFQNPFASLNPRKKIGHALEEPLAINTSLGRAEREERARAMMARVGLRPEHHQRYPHM
                     FSGGQRQRIAIARALMLQPDIVVADEPVSALDVSIQAQVLNLLMDLQEETRVAYVFIS
                     HNLAVVELIADDVLVMYLGKVVEHAAKRELFDRPRHPYTRALLASTPRIDRAARRQRQ
                     VLGGELPSPLHPPPGCAFHKRCPHAQARCAAEVPLLRTPEDGLTSGRVACHFAEAIAK
                     LPAP"
     gene            complement(2156..3139)
                     /locus_tag="VFB53_00190"
     CDS             complement(2156..3139)
                     /locus_tag="VFB53_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN85177.1"
                     /translation="MALLDVQDLTVEFGPEASPFKAVDGVSFAVDAGEVVGIVGESGS
                     GKSVTALALMGLVDAPGRVRASRLAFDGRDVLAMRERERRAMLGRDIAMIFQDPMSSL
                     NPCFTVEHQLAETLRIHEGGSRRALRARALELLRAVEIPDAERRLEAYPHQLSGGMSQ
                     RVMVAMAIACNPRLLIADEPTTALDVTIQAQMLALLLKLQRERGMGMVLITHDLGVVA
                     EVARRVMVMYAGQVVETAPVPDIFAAPHHPYTEALLAALPEHNIGRRRLRSMPGVVPG
                     ALDRPSGCLLAPRCAYAQPRCVAQRPALDGVEGAQVRCFFPLRSRAVETTR"
     gene            complement(3150..4037)
                     /locus_tag="VFB53_00195"
     CDS             complement(3150..4037)
                     /locus_tag="VFB53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN85178.1"
                     /translation="MTDATLAALPPSPWVELWRDFRRNRGALIGLGIVAVLLLAALLA
                     DVIAPHPPAEQFREATLRAPSWEPVDGRRFVLGTDPVGRDVLSRLIHGTRLSLLIGLV
                     SVGLSLSGGVLLGLVAGFFRGGIEFTIMRLMDIMLALPSLLLAVAVVAILGPGLMNAM
                     YAIAIVMLPHFVRLTRAAVIGELAKDYVAASRIAGASTPRLMFDTVLPNCAAPLIVQA
                     TLGFSTAILDAAALGFLGLGAQPPTPEWGSMLASALEFIQSAWWVVTLPGLAILVAVL
                     AFNLMGDGLRDALDPKLKR"
     gene            complement(4061..5068)
                     /locus_tag="VFB53_00200"
     CDS             complement(4061..5068)
                     /locus_tag="VFB53_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN85179.1"
                     /translation="MIAFLLKRLALVLPTFVGITLLVFALIRLLPGDPVEALSGERGM
                     TPERYARLLAEFGLDRPLPVQYLDYVWKALHGDLGFSTITHEPVFREFVARFPATIEL
                     SVVAMLLALAIGLPAGILAATKRNSVVDYTVMGASLAGYSMPIFWWGLLLILTFSVTL
                     GWTPVSGRIAIQYDIPSVTGFMLVDSLLASDEGAFRSALSHLILPSIALGTIPLAVIA
                     RMTRSSMLEVLREDYVRTARAKGASTFRVVAIHALRNALIPVVTTVGLQVGTLLAGAI
                     LTETIFSWPGIGKWLVEAIHRRDYPAVQGGILLSATVIVGVNLVVDLLYGVINPRIRH
                     H"
     gene            complement(5148..>6028)
                     /locus_tag="VFB53_00205"
     CDS             complement(5148..>6028)
                     /locus_tag="VFB53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85180.1"
                     /translation="TTDASVRYAKLKAGECHAMAFPKPADVALMKNDKNINLVQKEGL
                     NVGYIAFNVEKKPFDNKLVRQALGMAVNKKAILDAVFQGAGQVAKNPIPPTLWSYNDK
                     IKDHPYDPAKARELLAKAGFASGTEVELWYLPVTRPYNPDGKRMAELIQADWEKVGVK
                     TKLLTFEWGEYRKRSKTGEQHAMMFGWSGDNGDPDNFFVPLLGCAAVKGGGNVARWCH
                     KDFEDLIQKAKTQTKQADRAALYQKAQEIFKEEAPWIPIAHSVRFDPVRKEVTGYKMD
                     ATAHHFFHKVDLASPK"
BASE COUNT          852 a         2136 c         2204 g          836 t
ORIGIN      
        1 cacgcgcatc tggcccacgc tgatgccgct ggggcggcgg cgcaggttcg tgatctcgcc
       61 gatcgggtcg ggcatggcgg cgatctgctc gcagccttcg gccaccgtcc cgatgatctt
      121 cggggtgagc ttcaggcgct cgaccatcgg gggggcgagg ccggcggatc gggcggcggc
      181 gatgtcgcgt tcgttgggcg cggcgagggc atcggcgtcg tcgcgcagcc ggcgcgcgag
      241 ggcgacgagg gccgcgttct tcgcggccgt ggaggccgcc gccatcctgg cgctcgccgc
      301 gcgcgcggcg gcgcccatgt gggcgatgac ggcctggaga tcggtggagg tcatgggggc
      361 gtattgtcct gcaacgcctg ggcccccacc cccgctatct cccgtacgtc cccgtgaacg
      421 tcgccgtgcg cagcttgttc gcgttgatca tgaaacccgc cagcgccgcc gtcgcgtccg
      481 ccgggaggtc cagcttctcc gtcttcagcg catgcagcgt gaacacgtag cgatgcggct
      541 tgtcgccctg cggcgggcac gggccgcccc agccgggctt gccgaaatcg gtgcgcacct
      601 gcagcgcgcc cgcgggcagc ctgcctgcgc cggtgccggc gccttcgggc agctccgtca
      661 cgttggccgg gatgttcacc accacccagt gccaccagcc cgagccggtg ggcgcgtcgg
      721 ggtcgtacac cgtgagcgcg tagctccggg tgcccttcgg cgcgccgctc cacttcagcg
      781 ccggcgagac gttctgcccc gtgcagccga agccgtcgaa gacctgcgcc tcggcgatgc
      841 gcgcgccggg cttgatcgtc gggctggcga gcgtgaagcc ggcggcctgg gcgaggccgt
      901 tgcacgccag gatggcggcg gtgcacgaca cggtgcgggc gaagcgtggc atggaagggc
      961 ctttccttcg gggttgcgtt ggcgggccgg tactctgcac cgcgcgcggc gcggattcaa
     1021 gggcgcggcc ggtgcgacgg cggcacgctt cgcggcgccg gtgcggggcg ggcacgcttc
     1081 gcgcggcgcc gaggcggcgg cgcgcaggct tcgcgcagca ccgtgcggcg ccgcgcgaca
     1141 ccctacggcg ccggcagctt cgcgatcgcc tcggcgaagt ggcacgccac gcggccgctc
     1201 gtgagcccgt cctccggcgt gcgcagcagc ggcacctcgg ccgcgcagcg cgcctgcgcg
     1261 tgcgggcagc gcttgtggaa ggcgcagccc ggcggcggat gcaggggcga cggcagctcg
     1321 ccgcccagca cctggcgctg gcggcgcgcc gcacggtcga tgcgcggcgt gctggcgagc
     1381 agcgcgcgcg tgtacggatg ccgcggccgg tcgaacagct cccgcttggc cgcgtgctcc
     1441 accaccttgc cgaggtacat cacgagcacg tcgtcggcga tcagctccac caccgcgagg
     1501 ttgtgcgaga tgaagacgta ggcgacgcgc gtctcctcct gcaggtccat gagcaggttg
     1561 agcacctgcg cctggatcga cacgtcgagc gccgacaccg gctcgtcggc caccacgatg
     1621 tccggctgca gcatgagcgc gcgcgcgatc gcgatgcgct gccgctgccc gcccgagaac
     1681 atgtgcggat agcgctggtg gtgctcgggg cgcaggccga cgcgggccat catcgcgcgc
     1741 gcgcgctcct cgcgctcggc gcgcccgagg gaggtgttga tcgccagcgg ctcctccagc
     1801 gcgtggccga tcttcttgcg cgggttgagg ctggcgaacg ggttctggaa caccatctgc
     1861 acctggcgcc gcagcgcgcg ccgggcggcg gtgtcggcat gcgctacgtc caggccgccg
     1921 atgcgcaacg tgccggcggt gggcggctcg atcatcgtca cctggcgcgc cagcgtgctc
     1981 ttgccgcagc cggactcgcc cacgacggcg agcgtgcggc cgcgctccag cgtgaaggag
     2041 acgccgtcga gcgcgcgcac cgtggccttc ggccggagga agccgaggga caccgtgtag
     2101 tgcctggcca ggccctcggc cgcgagcacc acggcgggcg acggcggcgc ggcgctcatc
     2161 gcgtcgtctc cacggcacgc gaccgcaagg ggaagaagca gcgcacctgt gccccttcca
     2221 ccccgtccag cgccggccgc tgcgccacgc agcgcggttg cgcatacgcg cagcgcggcg
     2281 ccagcaggca gccggacggg cggtcgagcg cgcccggcac cacgcccggc atcgagcgca
     2341 ggcgccgccg gccgatgttg tgctccggca gcgcggcgag cagcgcctcg gtgtaggggt
     2401 gatgcggcgc cgcgaagatg tccggcaccg gcgccgtctc caccacctgg cccgcgtaca
     2461 tcaccatcac gcgccgcgcg acctcggcca ccacgcccag gtcgtgcgtg atcagcacca
     2521 tccccatccc gcgctcgcgc tgcagcttca gcagcagcgc gagcatctgc gcctggatgg
     2581 tgacgtcgag cgcggtggtg ggctcgtcgg cgatcagcag gcggggattg cacgcgatcg
     2641 ccatcgccac catcacgcgc tggctcatgc cgcccgagag ctggtgagga taggcctcga
     2701 gccggcgctc ggcgtccggg atctccaccg cgcgcagcag ctcgagggcg cgggcgcgca
     2761 gcgcgcggcg cgagccgccc tcgtggatgc gcagcgtctc ggcgagctgg tgctcgacgg
     2821 tgaagcacgg gttgaggctc gacatcgggt cctggaagat catcgcgatg tcgcggccga
     2881 gcatggcgcg ccgctcgcgc tcgcgcatgg ccagcacgtc gcggccgtcg aacgcgaggc
     2941 gcgacgcgcg cacgcgcccc ggggcgtcga ccaggcccat cagcgcgagc gccgtcacgc
     3001 tcttgcccga gcccgactcg ccgacgatgc ccaccacctc gcccgcatcc accgcgaagc
     3061 tcacgccgtc caccgccttg aagggcgacg cctccgggcc gaactcgacc gtcaggtcct
     3121 gcacgtcgag cagcgccatc ggggccggcc tatcgcttca gcttggggtc gagcgcatcg
     3181 cgcagcccgt cgcccatgag gttgaacgcc agcaccgcga ccaggatcgc caggcccggc
     3241 agcgtgacca cccaccacgc gctctggatg aactcgagcg cgctcgccag catcgagccc
     3301 cattcgggcg tgggcggctg cgcgcccagg ccgaggaagc cgagcgcggc cgcgtcgagg
     3361 atggcggtgg agaagccgag cgtggcctgc acgatgagcg gcgcggcgca gttgggcagc
     3421 accgtgtcga acatcagccg cggcgtgctc gcgccggcga tccgcgacgc ggcgacgtag
     3481 tccttcgcca gctcgccgat caccgccgcg cgcgtgagcc gcacgaagtg cggcagcatc
     3541 acgatggcga tggcgtacat cgcgttcatc aggcccgggc cgaggatggc caccaccgcc
     3601 accgcgagca gcaggctcgg cagcgccagc atgatgtcca tgagccgcat gatggtgaac
     3661 tcgatgccgc cgcggaagaa gccggccacg agccccagca gcacgccgcc cgagagcgag
     3721 agccccaccg agaccagccc gatcagcagc gacaggcgcg tgccgtggat gaggcgcgag
     3781 agcacgtcgc ggcccaccgg gtcggtgccc agcacgaagc gccggccgtc caccggctcc
     3841 cacgacggcg cgcgcagcgt cgcctcgcgg aactgctcgg ccggcggatg cggcgcgatc
     3901 acgtcggcca gcagcgccgc gagcagcagc acggcgacga tgcccaggcc gatcagcgca
     3961 ccgcggttgc ggcggaagtc gcgccagagc tcgacccagg gagacggcgg cagggcggcg
     4021 agcgtggcgt cggtcatggc gtggcgcgcg gcatcggcgg tcagtgatgc cggatgcgcg
     4081 ggttgatgac gccgtacagc aggtcgacca ccaggttgac gccgacgatc acggtggccg
     4141 agagcaggat gccgccctgc accgcgggat agtcgcgccg gtggatcgct tcgaccagcc
     4201 acttgccgat gccgggccag gagaagatgg tctcggtgag gatcgcgccc gcgagcagtg
     4261 tgcccacctg caggcccacg gtcgtcacca ccgggatgag cgcgttgcgc agcgcgtgga
     4321 tcgccaccac gcggaaggtg gaggcgccct tggcgcgcgc ggtgcgcacg tagtcctcgc
     4381 gcagcacctc gagcatcgac gagcgcgtca tgcgcgcgat cacggcgagc gggatggtgc
     4441 cgagggcgat cgacggcagg atcaggtgcg acagcgcgct gcggaatgcg ccctcgtcgg
     4501 acgcgagcag cgagtcgacc agcatgaagc cggtgacgct cgggatgtcg tactggatcg
     4561 cgatgcgccc ggacacgggc gtccagccga gcgtgaccga gaaggtgagg atgagcagca
     4621 ggccccacca gaagatcggc atcgagtagc cggcgagcga ggcgcccatc accgtgtagt
     4681 cgaccacgct gttgcgcttc gtcgcggcga ggatgccggc cggcaggccg atcgcgagcg
     4741 cgagcagcat cgcgaccacc gacagctcga tggtggccgg gaagcgcgcg acgaactcgc
     4801 ggaacaccgg ctcgtgcgtg atggtggaaa agccgaggtc gccgtgcagc gccttccaca
     4861 cgtagtcgag gtactgcacc ggcagcggcc ggtcgaggcc gaattcggcg agcaggcgcg
     4921 cgtagcgctc cggcgtcatc ccgcgctcgc ccgacagcgc ctcgacgggg tcgccgggga
     4981 gcagccggat cagggcgaag acgagcagcg tgatgccgac gaaggtcggc aggacgaggg
     5041 cgaggcgctt gaggaggaag gcgatcatgg cgcgggtggg ctgggagacc tgccaccgtc
     5101 gccggccccc gccccggccc tccccctgcg ggggagggac aaaggcatca cttcgggctc
     5161 gccaggtcca ccttgtggaa gaagtggtgc gccgtcgcgt ccatcttgta gcccgtcact
     5221 tccttgcgca ccgggtcgaa gcgcaccgaa tgcgcgatcg ggatccaggg cgcctcctcc
     5281 ttgaagatct cctgcgcctt ctggtagagc gccgcgcggt cggcctgctt cgtctgcgtc
     5341 ttggccttct ggatcaggtc ctcgaagtcc ttgtggcacc agcgcgccac gttgccgccg
     5401 cccttgaccg cggcgcagcc cagcagcggc acgaagaagt tgtcggggtc gccgttgtcg
     5461 ccggaccagc cgaacatcat cgcgtgctgc tcgcccgtct tgctgcgctt gcggtactcg
     5521 ccccattcga aggtgagcag cttcgtcttc acgcccacct tctcccagtc ggcctggatc
     5581 agctcggcca tgcgcttgcc gtccgggttg tacgggcgcg tgaccggcag gtaccacagc
     5641 tccacctcgg tgccgctcgc gaagccggcc ttggcgagca gctcgcgcgc cttcgccggg
     5701 tcgtacgggt ggtccttgat cttgtcgttg tacgaccaga gcgtgggcgg gatcgggttc
     5761 ttcgccactt ggcccgcgcc ctggaacacc gcgtcgagga tggccttctt gttgaccgcc
     5821 atgccgagcg cctgccgcac gagcttgttg tcgaacggct tcttctcgac gttgaaggcg
     5881 atgtagccga cgttcagccc ttccttctgc accaggttga tgttcttgtc gttcttcatc
     5941 agcgccacgt ccgccggctt ggggaaggcc atcgcatggc actcgcccgc cttcagcttg
     6001 gcgtagcgca ccgacgcgtc ggtggtga
//
LOCUS       DASRIE010000007         5170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3129936, whole genome shotgun sequence.
ACCESSION   DASRIE010000007 DASRIE010000000
VERSION     DASRIE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129936"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFB53_00210"
     CDS             complement(<1..598)
                     /locus_tag="VFB53_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZN85181.1"
                     /translation="MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIVNPYDLFALEE
                     ALRLKDRFGGRVTALCMGPPQAEEALRKCVSFGATDAILVTDRAFAGADTLATSYALA
                     AAIRKVAQEQAVDLVFTGKQTIDGDTAQVGPGIAKRLGWQLLTYVSRIADLDPDAQRL
                     RVERRAEGGVQLLETRLPALVTMLEGTNEMRFAGMDDML"
     gene            complement(612..959)
                     /locus_tag="VFB53_00215"
     CDS             complement(612..959)
                     /locus_tag="VFB53_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HZN85182.1"
                     /translation="MDPLSERLRALSSAEDFLRFFGLPYDERVLQVNRLHILKRFHQY
                     LRGESELQAGGDDVALFRRYRELLARAYLDFVASTPAREKVFRVFEQAEGVRTVPLEA
                     LRASRAARSRTPA"
     gene            complement(976..2115)
                     /gene="nifV"
                     /locus_tag="VFB53_00220"
     CDS             complement(976..2115)
                     /gene="nifV"
                     /locus_tag="VFB53_00220"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HZN85183.1"
                     /translation="MSLQHFTLNDTTLRDGEQTAGVAFSPEEKLAIAKALEEAGVPEL
                     EVGIAAMGDAEVEGIRAVTGSLRRARSMVWARMADVDLEAALRCGADIVHMAIPVSDL
                     QITRKLQRTRHSVLQSIADFVDRAADAGATVSVGFEDASRADPSFVQQAAQVAQRHGA
                     VRVRYADTLGVIDPFQAFDRIADLKREIDIGIEFHAHDDLGLATANTLAALRAGATHA
                     STTVNGLGERAGNAALEEVVMAARHLHGFDCGVDTHALPAISALVARASGRPVAANKS
                     IVGEAVFTHESGIHVDGLLKDRRNYEPFAPEEVGRAHRLVLGKHSGSRGVMAAYARLG
                     IEIEPVRAQWLLACVRRHVTATKQAPLDADLLRFLQLLPVPAVAG"
     gene            complement(2307..2654)
                     /gene="fdx"
                     /locus_tag="VFB53_00225"
     CDS             complement(2307..2654)
                     /gene="fdx"
                     /locus_tag="VFB53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HZN85184.1"
                     /translation="MPGELTRIVVLPHPQLCPEGLAFEARGGRKLLDELLARGIAVEH
                     ACEKVCACATCHVHMREGRDAVAPPDEEEEDQLDEAWGVDADSRLACCVKVRGDRLVI
                     ELPRFSRNHARER"
     gene            complement(2657..4558)
                     /gene="hscA"
                     /locus_tag="VFB53_00230"
     CDS             complement(2657..4558)
                     /gene="hscA"
                     /locus_tag="VFB53_00230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HZN85185.1"
                     /translation="MALLQIAEPGRAAAPHQHRLAVGIDLGTTNSLVAAVRSGLPAVL
                     PDDLGRMLLPSIVHYGKDGVIVGHDARLRARADPGNTVASAKRLIGRAPADVEGAQLP
                     YALEAISASAVGIRTRAGLKSPVDVGAEVLRVLRERAEQSLGGALAGAVITVPAYFDD
                     AQRQATRDAAAQAGLHVLRLLNEPTAAAVAYGLESAREGTFAVYDLGGGTFDVSVLRL
                     ARGVFEVVATAGDARLGGDDFDHRLAAWFALATGGDWLPDIGALHAAARAAKEILTDH
                     EEATMACTRSGGVVESRRVTRALLGGLTRDLVDRTLAPVRRALRDARLKAAEVDGVVL
                     VGGATRMPQVRAAVAALFGREPLTGVDPDLAVALGAAIQADQLAGNRSGEDGLLLLDV
                     NPLSLGIETMGGLVEKIIPRNSTVPTARSQEFTTFKDGQTAMALHVVQGERELVAECR
                     SLARFTLGGIPPMVAGAARIRVTFQVDADGLLSVSAVEQGTGTQAQVEVKPSYGIAPE
                     EVSDMLRAALDQAGADAAKRGLREAEIDARRLLDATDAALREDGAQLLQPGEHADILR
                     VMQEVADLLEACAQGEGATPEALRATRARLRGAIEALNRVTTPFAGRRMDARVRQALA
                     GQKLARLAA"
     gene            complement(4561..4884)
                     /locus_tag="VFB53_00235"
     CDS             complement(4561..4884)
                     /locus_tag="VFB53_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZN85186.1"
                     /translation="MTLSVTPRAAAQIRKAIARRGGGFGLRLAVKTSGCSGYAYALEF
                     ADAVAGDDLAFESEGVQLLVAAGSLPLVDGTRLDWVREGLNEGFRFDNPNAANTCGCG
                     ESFAV"
     gene            complement(4899..>5170)
                     /gene="erpA"
                     /locus_tag="VFB53_00240"
     CDS             complement(4899..>5170)
                     /gene="erpA"
                     /locus_tag="VFB53_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZN85187.1"
                     /translation="EADPGVRLRIYVTGGGCSGFQYGFAFDDAAQEDDVQVVRGPVTV
                     VVDAMSLQYLVGAEIDYEDKLEGARFVIRNPNASTTCGCGSSFSV"
BASE COUNT          701 a         1863 c         1850 g          756 t
ORIGIN      
        1 gcagcatgtc gtccatcccg gcgaagcgca tctcgttggt gccttcgagc atggtgacga
       61 gcgccggcag gcgcgtttcc agcagttgca caccgccctc ggcgcggcgc tccacgcgca
      121 ggcgctgcgc atcgggatcg aggtccgcga tccggctcac gtaggtgagg agctgccatc
      181 ccaggcgctt cgcgatgccc gggccgacct gggcggtgtc gccgtcgatg gtctgcttgc
      241 cggtgaacac caggtccacc gcctgctcct gcgcgacctt ccggatggcc gctgccagcg
      301 cgtagctcgt ggccagcgtg tcggcgccag cgaaggcgcg gtcggtcacc aggatcgcat
      361 cggtggcccc gaagctcacg cacttgcgca acgcctcctc ggcctgcggc gggcccatgc
      421 acagcgcggt gacccggccg ccgaagcggt ccttcaggcg cagcgcctcc tccagcgcga
      481 acaggtcata ggggttcacg atcgccggga cgccctggcg catgatggtg ttggtgaccg
      541 gatggacgcg gatctgcgcc gagtcgggca cctgcttgat gcagacgacg atgtgcatgt
      601 cggggctcct gtcaggcggg ggtgcgggag cgcgcggcgc ggctcgcgcg cagcgcctca
      661 agtggcacgg tgcgcacgcc ctcggcctgc tcgaacacgc ggaagacctt ctcgcgcgcc
      721 ggcgtggagg cgacgaagtc caggtaggcg cgcgccagca gctcgcggta gcggcggaac
      781 agcgcgacgt cgtcgccgcc ggcctgcagt tccgactcgc cacgcaggta ctggtggaag
      841 cgcttgagga tgtgcagccg gttgacctgc agcacccgct cgtcgtaggg caggccgaag
      901 aagcgcagga agtcctcggc gctcgaaagc gcccgcaggc gttcgctcag ggggtccacg
      961 gaaactcctt ccatctcagc ccgccaccgc gggcacgggc agcaattgca ggaagcgcag
     1021 caggtccgcg tccagcggcg cctgcttggt cgcggtgacg tggcgccgca cgcaggccag
     1081 cagccattgc gcgcgcaccg gttcgatctc gatgcccagg cgggcgtagg cggccatcac
     1141 gccgcgcgag cccgagtgct tgcccagcac gaggcggtgc gcgcggccca cctcctccgg
     1201 cgcgaagggc tcgtagttgc gccggtcctt cagcagcccg tcgacgtgga tgcccgactc
     1261 gtgcgtgaac accgcttcgc ccacgatgct cttgttggcc gcgaccggcc gcccggaagc
     1321 gcgcgcgacc agggcggaga tcgccggcag ggcgtgggtg tcgaccccgc agtcgaaacc
     1381 gtgcaggtgg cgcgcggcca tcaccacttc ttccagtgcg gcgttgccgg cccgctcgcc
     1441 gaggccgttg acggtggtgc tggcatgcgt ggcgccagcg cgcagggccg ccagcgtgtt
     1501 ggccgtggcc agccccagat cgtcgtgcgc atggaactcg atgccgatgt cgatctcgcg
     1561 cttcaggtcc gcgatgcggt cgaacgcctg gaacgggtcg atgacgccca gcgtgtccgc
     1621 gtagcgcacc cgcaccgcgc catgccgttg cgcgacctgc gccgcctgct gcacgaacga
     1681 gggatcggcg cgcgaggcgt cctcgaaccc gacgctcacg gtggcgccgg cgtcggcggc
     1741 gcgatccacg aaatcggcga tggactgcaa taccgagtgg cgggtccgtt gcagcttgcg
     1801 cgtgatctgc aggtccgaca ccggaatcgc catgtggacg atgtctgcgc cgcagcgcag
     1861 cgccgcctcg aggtcgacgt ccgccatgcg cgcccagacc atgctgcggg cgcggcgcag
     1921 gctgcccgtg acggcgcgga tcccttcgac ctcggcgtcg cccatggcgg cgatgccgac
     1981 ctcgagctcg ggtacgccgg cttcctcgag ggccttcgcg atcgcgagtt tctcctcagg
     2041 gctgaaggcg acgccggcgg tctgctcgcc gtcgcgcaag gtggtgtcgt tgagggtgaa
     2101 gtgctgcagg gacatgcggg gctcctgatt gcagggctta ccgcaagggg catgccatgc
     2161 ggcccggatg acgcaagtgc ttgattcggc tggcatcttg cggtggcgtg gtgcggcggg
     2221 tgggctgtgg ggtttgtcgg cttgtgcaca tggccctgtg cgcttcctgc gtgccggagc
     2281 aggacaagag aggggcagcc ggggtcttac cgctcccgcg cgtgattgcg cgagaaccgc
     2341 ggcagctcga tcaccagcct gtcgccgcgc accttcacgc agcaggccag gcgcgagtcg
     2401 gcatcgacgc cccaggcttc atcgagctgg tcttcctctt cctcgtccgg cggcgccacg
     2461 gcatcgcggc cttcgcgcat gtgcacatgg caggtggcgc aggcgcagac cttctcgcac
     2521 gcatgttcca ccgcgatgcc gcgcgcgagc agttcgtcca gcagctttcg gccgccgcgc
     2581 gcctcgaacg caagtccctc cggacagagc tgcggatgcg gcagcaccac gatgcgggtg
     2641 agctcgcccg gcatgctcac gccgccaggc gcgcgagctt ctggcccgcc agcgcctgcc
     2701 gcacgcgcgc gtccatacgt cggccggcga aaggcgtggt gacccggttg agggcctcga
     2761 tggcgccgcg caggcgggcg cgcgtcgcgc gcagcgcctc cggcgtggcg ccctcgcctt
     2821 gcgcgcacgc ctccagcagg tcggcaacct cctgcatcac gcgcaggatg tccgcgtgtt
     2881 cgcccggctg cagcagctgt gccccgtcct cgcgcagcgc cgcgtcggtg gcatcgagca
     2941 gccggcgggc gtcgatctcg gcttcgcgca ggccgcgctt ggcggcgtcc gcgccggcct
     3001 ggtccagcgc agcgcgcagc atgtcgctga cttcctcggg ggcgatcccg tagctgggct
     3061 tgacttccac ctgggcctgg gtgcccgtgc cctgctcgac cgcgctcacc gacagcaggc
     3121 cgtcggcgtc gacctggaag gtgacgcgga tgcgcgccgc gcccgccacc atgggcggaa
     3181 tgccgcccaa tgtgaagcgg gcgagcgaac ggcactcggc gaccagctcg cgctcgccct
     3241 gaacgacgtg cagcgccatg gccgtctggc cgtccttgaa ggtggtgaac tcctgggacc
     3301 gcgcagtcgg cacggtgctg ttgcgcggaa tgatcttctc caccagcccg cccatggttt
     3361 cgatgccgag cgacagcggg ttgacgtcca gcagcagcag cccgtcttcg ccggatcggt
     3421 tgccggcgag ctggtcggcc tggatcgcgg cgccgagtgc caccgcgagg tcggggtcga
     3481 cgccggtgag cggctcgcgg ccgaacagcg cagcgacggc cgcgcgcacc tgcggcatac
     3541 gcgtcgcccc gccaaccagc accacgccgt ccacctccgc ggccttcagg cgcgcgtcgc
     3601 gcaatgctcg ccgcaccggc gccagcgtgc ggtcgaccag gtcgcgcgtg agaccaccca
     3661 gcagcgcgcg cgtgacccgc cgcgactcca ccacgccgcc gctgcgcgtg caggccatcg
     3721 tggcctcctc gtggtcggtc aggatctcct tggccgcgcg ggcggccgcg tgcagcgcgc
     3781 cgatgtcggg cagccagtcg ccgccggtgg ccagggcgaa ccaggccgcc aggcgatggt
     3841 cgaagtcgtc gcctcccagc cgcgcgtcgc cggcggtggc caccacctcg aacacgccgc
     3901 gcgccagccg cagcaccgag acgtcgaagg tgccgccgcc caggtcgtac acggcgaagg
     3961 tgccttcccg cgcggattcc agcccgtagg ccacggcggc ggccgtcggt tcgttcagca
     4021 gccgcaggac atggaggccg gcctgcgccg cggcgtccct ggtggcctgg cgctgcgcgt
     4081 cgtcgaagta ggcgggcacc gtgatcaccg cgccggccag cgcgccgccc aggctttgct
     4141 ccgcgcgttc gcgcagcacc cgcagcacct cggcgccgac gtccaccggg ctcttcaggc
     4201 cggcgcgggt gcggatgccg accgcggacg ccgagattgc ctccagcgcg taaggcagct
     4261 gcgcgccttc gacgtcggcc ggcgcgcgcc cgatcagccg cttggccgag gcaaccgtgt
     4321 tgcccgggtc ggcgcgcgcg cgcagtcgcg catcgtggcc cacaatgacg ccgtccttgc
     4381 cgtagtggac gatcgagggc agcagcatcc gcccgaggtc gtcgggcaac accgcgggca
     4441 ggccgctgcg caccgcggcg acgagcgagt tggtggtccc caggtcgatg cccacggcca
     4501 gccggtgctg gtgcggggcg gcggcgcgtc cgggctcggc gatctgcagc agcgccatgg
     4561 tcagaccgcg aagctttcgc cgcagccgca ggtgttcgcg gcgttgggat tgtcgaacct
     4621 gaaaccctcg ttcaggccct cgcgcaccca gtccaggcgc gtgccgtcga ccaggggcag
     4681 gctgccggcc gccaccagca actgcacgcc ctcgctctcg aaggccaggt cgtcgcccgc
     4741 taccgcgtcg gcgaattcga gggcgtaggc gtaaccggag cagccgctcg tcttgacggc
     4801 cagtcgcagg ccgaagccgc cgccacggcg ggcgatggcc ttgcggatct gggcggcggc
     4861 acggggcgtc acgctgaggg tcatgcgcat tccccttttc agaccgagaa cgaactgccg
     4921 caaccacagg tcgtgctggc gttcgggttg cggatcacga agcgcgcgcc ctccagcttg
     4981 tcctcgtagt cgatctcggc gcccacaagg tactgcaggc tcatggcgtc gaccacgacg
     5041 gtcaccggac cgcgcaccac ttgcacgtca tcctcctgtg ccgcgtcgtc gaaggcgaag
     5101 ccgtactgga agccggagca gccgccgccg gtgacgtaga tgcgcaggcg cacgccggga
     5161 tcggcctcct
//
LOCUS       DASRIE010000008         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1311307, whole genome shotgun sequence.
ACCESSION   DASRIE010000008 DASRIE010000000
VERSION     DASRIE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311307"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFB53_00245"
     CDS             complement(<1..462)
                     /locus_tag="VFB53_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN85188.1"
                     /translation="MEAAAAPWWIWPLALFALTYLLGIVAVVAGIGGGVLFVPIVGSF
                     FPFHLDYVRGAGLVLALAGTLAAAPLLLRARLSNLRLVLPLALAGSIASLCGAVVGLA
                     LPTNVVQVALGAVIVGVALLMWRTDTHGRLALESDALGELLRLKGEFHDPAA"
     gene            486..1265
                     /gene="otnI"
                     /locus_tag="VFB53_00250"
     CDS             486..1265
                     /gene="otnI"
                     /locus_tag="VFB53_00250"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HZN85189.1"
                     /translation="MPKLAANLSTLFTEVDFLERFGAAARAGFRGVEYQYPYEWPAPD
                     IAAAARDAGVEVVLHNMPMGDAQRGERGTACLTGREPRFREDLERAIDYARAARCPNL
                     HLMAGVAPPGADRAALHATYVSNLRHAAQRLARERMRALIEPLSERTVANCFLRSSAQ
                     AAQVLGEVAADNAFIQYDLFHMQIMEGNLAATLERLLPRIGHVQIADVPHRHEPGSGE
                     INFDFLLRHLDALGYSGWVGCEYNPKGDTVEGLKWARPYLS"
     gene            1241..2698
                     /locus_tag="VFB53_00255"
     CDS             1241..2698
                     /locus_tag="VFB53_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN85190.1"
                     /translation="MGAALPVVKELLQFLPQRAVLHQEEDTRPYECDGLTAYRQLPMV
                     VALPETEEQVRRILKTCASMKIPVVPRGAGTGLSGGALPPGDGVLLSMAKFMRVLRID
                     PLARVAVVQPGVRNAVVSELAAPHGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGL
                     TLHNVLGMRGFTIDGEPIELGGEAPDAPGYDLLALAIGSEGMLMVVTEVTLKLLPKPH
                     SARVIMASFDDVEKAGAAVADIIAAGIVPAGLEMMDQPATRAVEQFVSAGYDLEAAAI
                     LLCESDGSVEEVEDEIARMKAVLERAGATRMTVSRDEAERLRFWSGRKAAFPAVGRIS
                     PDYYCMDGTIPRKRLGEVLRFIAAMEKKYALRCPNVFHAGDGNLHPLILFDANDADQL
                     HRTELFAAEVLEKCVEVGGTITGEHGVGVEKINQMCVQFRPDELELFRAVKRSFDPHG
                     LLNPGKAVPTLARCAEYGRMRVHGGQLPHPDLPRF"
     gene            2695..>3072
                     /locus_tag="VFB53_00260"
     CDS             2695..>3072
                     /locus_tag="VFB53_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN85191.1"
                     /translation="MTEIFQDQIKQAAARRAPLRLRGGGSKDFYGDVLRGEVLDTRGY
                     AGIVAYEPSELVVTARAGTRLADLEAALSGNDQMLPFEPPHFGPDATVGGCVAAGLSG
                     PRRTAAGALRDFVLGLKLVDGQGR"
BASE COUNT          480 a         1069 c         1085 g          438 t
ORIGIN      
        1 cgcggcgggg tcgtggaatt cgcctttcag ccgcaacagc tcgccgagcg cgtcggactc
       61 gagggcgagc cggccgtgcg tgtcggtgcg ccacatgagg agtgcaacgc cgacgatcac
      121 cgcgcccagc gcgacctgga ccacgttggt cggcagcgcg aggccgacca ccgcgccgca
      181 cagcgacgcg atcgatccgg cgagcgcgag cggcagcacc aggcgcaggt tggagagccg
      241 ggcgcgcagc agcagcggcg ccgcggccag ggttcccgcg agcgccagca ccagccccgc
      301 gccgcgcacg taatcgaggt ggaagggaaa gaagctgccg acgatcggga cgaacagcac
      361 cccgccgccg attcccgcga ccaccgcgac gatgccgagc aggtaggtca gcgcgaacag
      421 cgcgagcggc cagatccacc agggggcggc tgcggcttcc atgttcttcg ggctatgctg
      481 gcattatgcc caagctcgcc gcgaatcttt ccaccctgtt caccgaggtg gatttcctcg
      541 agcgcttcgg tgccgcggcg cgcgccggct tccgcggcgt cgagtaccag tatccctacg
      601 agtggccggc gccggacatc gcagcggcgg cgcgcgacgc cggtgtcgag gtggtgctgc
      661 acaacatgcc gatgggcgac gcgcagcgcg gcgagcgggg caccgcctgc ctgaccgggc
      721 gcgagccgcg cttccgcgag gacctcgagc gggcgatcga ctacgcgcgc gcggcgcgct
      781 gccccaacct gcacctgatg gcgggcgtgg cgccccccgg ggccgaccgt gccgcgctgc
      841 acgccaccta cgtctcgaac ctgcggcacg cggcgcagcg gctcgcgcgc gagcgcatgc
      901 gcgcgctgat cgagccgctc agcgagcgca ccgtcgccaa ctgcttcctg cgctcgagcg
      961 cgcaggcggc gcaggtgctg ggcgaggtcg cggcggacaa cgccttcatc cagtacgacc
     1021 tgttccacat gcagatcatg gagggcaacc tcgccgcgac gctcgagcgc ctgctgccgc
     1081 gcatcggcca cgtccagatc gccgacgtgc cgcaccgcca cgagcccggc agcggcgaga
     1141 tcaacttcga ttttctgctg cgccatctcg acgcgctcgg atactcgggc tgggtgggat
     1201 gcgaatacaa tccaaagggt gacacggtcg aaggactgaa gtgggcgcgg ccctacctgt
     1261 cgtaaaggag ctgctgcagt ttctcccgca gcgagcggtg ttgcaccagg aagaagacac
     1321 gcggccgtac gaatgcgacg gcctgactgc ctatcgccag ctgccgatgg tggtggcgct
     1381 gcccgagacc gaggaacagg tgcgccgcat cctgaagacc tgcgcttcga tgaagattcc
     1441 cgtcgtgccg cgcggcgcgg ggaccgggct ctcgggcggc gcgctgcctc cgggcgacgg
     1501 cgtgctgctg tcgatggcga aattcatgcg cgtgctgcgc atcgatccgc tggcccgggt
     1561 ggcggtagtg cagcccggcg tgcgcaacgc cgtggtgtcc gagctcgcag cgccgcacgg
     1621 cctgtactac gcgcccgatc cttcgagcca gatcgcctgc accatcggcg gcaacgtctc
     1681 cgagaactcg ggcggcgtgc actgcctgaa atacggcctc accctgcaca acgtgctcgg
     1741 catgcgcggc ttcaccatcg acggcgagcc gatcgagctc ggcggcgagg cgcccgatgc
     1801 gccgggctac gacctgctcg cgctcgccat cggctcggaa ggcatgctga tggtggtgac
     1861 cgaggtgacc ctcaagctgc tgccgaaacc tcactccgcg cgcgtcatca tggcttcatt
     1921 cgacgacgtg gaaaaggccg gagccgcggt cgccgacatc atcgccgcgg gcatcgtccc
     1981 cgccggcctc gagatgatgg atcagccggc gacgcgcgcg gtcgagcagt tcgtgtccgc
     2041 gggctacgac ctggaggccg ccgcgatcct gctgtgcgag tccgacggca gcgtggagga
     2101 agtggaggac gaaatcgccc gcatgaaggc cgtcctcgag cgcgccggcg cgacccgcat
     2161 gacggtttcg cgcgacgagg ccgagcgcct gcgcttctgg tcggggcgca aggccgcgtt
     2221 tccggcggtc ggccgcatct cgcccgacta ctactgcatg gacggcacga tcccgcgcaa
     2281 gcgcctcggc gaggtgctgc gcttcatcgc cgcgatggag aagaagtacg ctctgcgctg
     2341 cccgaacgtt tttcatgccg gcgacggcaa cctgcacccg ctgatcctgt tcgacgccaa
     2401 cgatgccgac cagctgcacc gcacggagct gtttgccgcc gaggtgctgg aaaaatgcgt
     2461 ggaagtcggc ggcaccatta ccggcgagca cggcgttggc gtggaaaaga tcaaccagat
     2521 gtgcgtccag ttccgccccg atgagctcga gctgttccgc gcggtgaagc gcagcttcga
     2581 cccgcacggc ctgctcaacc cgggcaaggc ggtgccgacg ctcgcgcgct gcgccgagta
     2641 cgggcgcatg cgggtgcacg gcggccagct gccgcatccg gacctgccgc gcttttgaca
     2701 gagatttttc aagaccagat caagcaggcc gcggcgcgcc gcgccccgct acggctgcgg
     2761 gggggcggca gcaaggactt ctatggcgac gtgctgcgcg gcgaggtcct cgacacgcgc
     2821 ggctatgccg gcatcgtcgc ctatgaaccg agcgagctcg tagtgacggc ccgcgcgggt
     2881 actcgcctcg ccgatctgga ggcggcgctg tccgggaacg accagatgct tccgttcgag
     2941 ccgccgcatt tcggcccgga cgccacggtc ggcggctgcg tcgccgccgg gctgtcgggg
     3001 ccgcggcgca ccgccgcggg agcgctgcgc gatttcgtgc tcgggctgaa gctcgtcgac
     3061 gggcagggac gc
//
LOCUS       DASRIE010000009        25075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1829445, whole genome shotgun sequence.
ACCESSION   DASRIE010000009 DASRIE010000000
VERSION     DASRIE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829445"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1649
                     /locus_tag="VFB53_00265"
     CDS             66..1649
                     /locus_tag="VFB53_00265"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN85192.1"
                     /translation="MSARSHKAGLLVAVTLSGMAVLIIEILATRMLAPFFGNSIFTIS
                     SVIGVVLAALGIGYYLGGALAERRPSAAWFFSLIVAAGFAVLLLQLLNAALLPGLAYR
                     LSLVNGPLLVSLLMFFLPALFLAMLSPFAVALLHAREPGRGAGHAAGLVFFWSTLGSI
                     AGSLGTGFLLVPRWGIGSIVLGVGAGLVLLGGAGLLATRGVPRVLPAGLALLALACGA
                     ALRQVPADEGRGVVYAADGLYERIVIRELPLRGRTARVLLQDRNVSGGLYLDDGSMAF
                     DYTKYYELYRLFTPELTAALAIGGGAYNVPAALLRGAPRAIVDVAETDPSLHALALRY
                     FALPDDARLRNHVIDGRRFLHDTEERYDLVFADAFRDFVSMPAQFASREFFSLARSRL
                     REDGVLIANYYGSLAPDSRAMLYSMLRTMRAAFPQVYLLATLGPQSEELQNFIFIGHN
                     ASPAERRIDLRQAAAVAFAHPELKQVAALELRPAEALLEAHAVLTDDFAPVEYHAAGA
                     IRRYDATGRRAASSRSRTP"
     gene            complement(1606..2709)
                     /locus_tag="VFB53_00270"
     CDS             complement(1606..2709)
                     /locus_tag="VFB53_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZN85193.1"
                     /translation="MLQKALSRFLASALFLYAGAALPFTLILPPDEQTSPSNTITEAT
                     TVERQAAPIAGAIRAQLLGLRRAKGSRGQAQAGRVLVASAQADSRGDFSLLAQGLKEP
                     SAAGGGGDSASLWISAAANSLENTFSRTAFYGATHNLLAGFDMTRSEKFVFGVAAGHE
                     ASNFTTTFNAGNERTRGFNLAPYFSYLLTPAWTLDASAGYGEFDTRQSRTVGLAPLVS
                     VPVDSKFDSTREFASVNLTNVSTWGGWTLTGALGLLAAKRKHDAYTESDGTAVAASSQ
                     SVEQGNLLAEAAYGRGASEFYLGALYEKTRDPLQVQFATGAQPANDDDSTLLTAGWRH
                     FGKGLTANFVFSQRVGQDQVREYGFSMMLRVDL"
     gene            complement(2764..3555)
                     /locus_tag="VFB53_00275"
     CDS             complement(2764..3555)
                     /locus_tag="VFB53_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN85194.1"
                     /translation="MSAKADAKGPVQVIDRMMKLLDVLAAHPEPLGLKQIAQYTALHP
                     STAHRILSALAGGRLVDRVEPGSYRLGMRLLELGNVVRSGISVRELALPVMRELHAHT
                     GETVNLSVRHDDEIVYVERTSSGRTAMRVVHVIGARAALHITAAGKLFLLEDGFARLR
                     EYAKRTGLAAHTRNTLGNLQLLERDLERTQRQGWATDNEEAELGVRCVAAGVRDDNGQ
                     LIAALSLSTPADRMKPQWGQLIKETAERISRGIGHRPAPGRIGAA"
     gene            3616..4755
                     /locus_tag="VFB53_00280"
     CDS             3616..4755
                     /locus_tag="VFB53_00280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZN85195.1"
                     /translation="MPVVTNIEDLRRMAQKRVAKAIFDYVDRGAYDEQTLRANRADLE
                     ALHFRQRVAIDVDRRSTRGRMLGEEVAMPVAIAPVGLSGLNWADGEILGARAAQAFGV
                     PFTLSTMSICSIEDVAGAVARPFWFQLYVMRDRGFAASLIERAKAAGCTAIVLTLDLQ
                     IQGQRHMDLKNGLAVPPQLTLGTLLDVMRKPGWALNVLSGRRKSFGNLAGRIPDANSL
                     TTLSQWIAGQFDPTLSWKDVEWVKGLWGRKLVLKGILDAEDAKTAASSGADAIVVSNH
                     GGRQLDGTVSSIRALPEVVQAVGGRTEVWMDGGIRSGQDVLRALALGATGTMIGKSYA
                     YGLGAMGEAGVTKALEIIQKELDVTMALCGLRDVKDASPAILRGA"
     gene            complement(4718..6595)
                     /gene="mnmC"
                     /locus_tag="VFB53_00285"
     CDS             complement(4718..6595)
                     /gene="mnmC"
                     /locus_tag="VFB53_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HZN85196.1"
                     /translation="MPPAPLAPARLAFAADGTPYSEAYGDLYHSADGGPGQARHVFLG
                     GNGLPARWRGALRFTILETGFGLGLNFLAAWQAWRDDARRCERLHFVSVEKHPFTAQD
                     LAVLHAPHPEYAALAEELRSAWPLLVPGLHRLEFEEGRVVLTLAFGDAQALVPQLRLA
                     ADAFFLDGFAPAKNPELWSPELLRALSRLAAPGATAATWSVAAPVREGLRAAGFVAEK
                     RRGYARKSEMLVARFVPLRSLRTAPARPAERSAIVVGAGIAGAAAAERLAARGWEVTV
                     VERHLEPALEASGNHAGAFHPVVSPDDSHIARLTRAAFSFLLEHWRQFDAIGAAPEWR
                     RCGVLQLARDAREDASQAAALAALGFPEAFAQRLDRARASECAGVRLAAGGLWFPEGG
                     WIRPRSFARALIERSGARACYGREAAALERAGERWILRDSEGEAIAEAGAVVLANAAD
                     ALRLAPAKHIALRRVRGQLTHLPPIEALRCVLLRGGMLLPAIDGVSVTGASFDLEDAD
                     ASVRAESHAGNLERLERILPGASGGLDAATLQGRTSFRAVAADRLPVIGGLEETGGLY
                     AAFAYGSRGLLWAGLGGELLASLMEGEPLPLEGALAEALAPGRFAARARRRAGSPGSR
                     P"
     gene            complement(6600..7340)
                     /locus_tag="VFB53_00290"
     CDS             complement(6600..7340)
                     /locus_tag="VFB53_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85197.1"
                     /translation="MDLFGLQGRTALVPGGYGAIGGAICRGLARAGARVIVAGRDAGK
                     AAALAKKLGRGARGVALDVEDVAAIGRVVASLGRIDILVNCVGMQREQLLADVTEEAF
                     DAIYRTNLRAAMFLAQAVAKRQKRGGKQVHLLSVRAQLGLRGRGYSAYCSTKGGLAML
                     IRQHASELGRRGICVNGIAPTVVRTKMGAHWLKNPKTRAWLKERIPLGRIAEPADCVG
                     AALFFCSSASDYVTGQILYLDGGITASQ"
     gene            complement(7343..8041)
                     /locus_tag="VFB53_00295"
     CDS             complement(7343..8041)
                     /locus_tag="VFB53_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85198.1"
                     /translation="MKTELRGRTAVVTGAAGTMGRAVVRALAADGLRVVGIDVKAHQG
                     GVVCDISDPQAVARAVRGIGRVDVLVNNAGILSNNKSQDTAPEEWRRVLAANLDGAFY
                     LSRAVIPGMKQRRWGRIVNTCSLAAKTGGLTAGTAYSASKGALTSLTFSLARELAPFG
                     VTVNGISPAYVNTPMVTEQLSAAQRKAVLAQIPVGRFCEPEEFAHVVRFLVHPLAGFI
                     TGEIVDLNGGVVMD"
     gene            complement(8031..8909)
                     /locus_tag="VFB53_00300"
     CDS             complement(8031..8909)
                     /locus_tag="VFB53_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN85199.1"
                     /translation="MDVLLNLLPHLLNGVSLGLLFALIALGFMLIVGVMEVINLAHGS
                     LFALGAYVAITLMGWKLSYAAAFLLAPLLVGAFGMLLELCLRRTYGKDPLYGLLLTFG
                     AALVIEELIRVLWGSNEKQLPVPEMFSGATLVGGLLYAKYRMFACAAAVGLILLLWLF
                     LEKTPYGAMIKAGAHDSEMVRALGINLPRLRLAVFALGTALAAVAGIVLAPIWGVRPH
                     MGVDAVVPAFLIIVLGGVGSFWGAVLAGILVGIVVGLTGAYASEWSLLSMYLLLIALV
                     TFRARGLFGKKTALEN"
     gene            complement(8913..10187)
                     /locus_tag="VFB53_00305"
     CDS             complement(8913..10187)
                     /locus_tag="VFB53_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85200.1"
                     /translation="MQGNRRKLLQMLAAGAAFYGPWKVNRAWAQAGQKKPLVIGLTMD
                     ASGQYGASGMDERLGAMLAIREFNQKGGVLGRQIEALHMDTETTPATGSRVAERMITR
                     NDAAFLIGAVHSGVANAISQVAQKYGCVFFNTNSSSPTEAGRDCHRVKFVWDGNGTNF
                     AHAIVKNAMRASGRNWLLLTNDYVWGHNTSKATRTLVEANGGKIVDELMVPQNTRDFT
                     SYLLKIQQIKPNVIAAAVGGDDIKALRQQVVQTKLDKSFAWINNQQDWPDVYGLGPDA
                     VFGVFGTTWYYRLDLPGVKDFVARYQKTYPGIQIAVPGNVFYNGYVATRELLRCVEEA
                     GSTNNLAVIKKLEGRKMSAADRMQHHDAWIDASTHQVQQTIYMASYNDKPAEKDDIFR
                     IQAQVAPQDVVDQGAPAACKLESYAATPSYEQ"
     gene            complement(10199..11089)
                     /locus_tag="VFB53_00310"
     CDS             complement(10199..11089)
                     /locus_tag="VFB53_00310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85201.1"
                     /translation="MQRIGFIGIGLMGHGMAKNLLAKGHPLAFLAHRNRARLGDLLEL
                     GARELKSSAEIARESDVVILCVTGTPQVEANVYGGLLEAARQGQVVIDCSTSLPESTE
                     RIRADFAKKGVPYVDAPLARTPVQAEEGKLNCMVGADEKTLAAIRPVLEKFCENIFHV
                     GGPGAGHKVKLVNNFLALGTAALVCEALAACAKVGIDLETFYKLISAGGANSAMFQMI
                     VPKALQGDHGGLLFTLANARKDIGYYQGMVANAPFAGSMGAALMQSLVQANALGLGDK
                     FVPSLIEAHEKVNGIRVGKH"
     gene            complement(11056..12885)
                     /gene="iolC"
                     /locus_tag="VFB53_00315"
     CDS             complement(11056..12885)
                     /gene="iolC"
                     /locus_tag="VFB53_00315"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HZN85202.1"
                     /translation="MAERPLDLICMGRAAVDLYGEQVGARLEDVASFAKYLGGCPANI
                     AVGAARLGLKVAMLTRVGDEHNGRFVRETLAAEGVDVSHVATDARRLTALVFLAIRDR
                     DTFPLVFYRHDCADMAIAAEDFDPAFIGSAQALLVSGTHFSQPQTAAVCLRAIRQARR
                     FVFDIDYRPVLWGLASPGMGEQRFVASQHVTAKLQEVIPLAQLVIGTEEEFHIAGGCE
                     DTDEALRRLRRISAATFVVKRGPLGCSVHAGEQSLEGQGFPVEVFNVLGAGDGFAAGF
                     LSGWLRDLPPGECARRANACGALVVSRHGCAPAMPSQEELALFLSRRDWPHRLRESPE
                     LNHLHRVTTGRRPWPEVMALAFDHRTQLEELGPPKRIRRFKRLVAEGLVRARVPGAGA
                     IVDDRHGEEALFALAGSGLWVARPVEAPGSRPLAFEAGANVGVALRGWPAQHVVKCLV
                     HYEPRDLPALRQLYAACVDTGHELLLELLSRNPPLAEIYAAGVKPDWWKLAPPASDAE
                     WRAIAGSIARHDPHCRGVLLLGMEADEQELERGFALAARHPVCKGFAVGRSIFMDAAR
                     RWFRGACGDAQVVDEVAANYARLAGLWRKQRTLCKESVSSASA"
     gene            complement(12878..13711)
                     /locus_tag="VFB53_00320"
     CDS             complement(12878..13711)
                     /locus_tag="VFB53_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZN85203.1"
                     /translation="MPGARSFESLKTDLSRRYPGLSPQLQAIATYALENPQEMALDTV
                     ARLAKGAGVQPSAMVRFAQALGYGGFSDLQRVFRTRLVERSNHSPGLRNYRERIEAFR
                     AAHAKSGPVAVLDEFIDDGIASLEQLHEHVSGADLQAAVTLLAAAQSVYVLGHRRSFP
                     VAYYLSYALTQLELRAPLLDAVGGMLKEPARLIGAKDALLVVSFKNYTPEVVEVAQAC
                     RGRKVPVVAITDNALSPLARAATVCFQIEEKTSRPFRMLVAPMCLAQALVVALGEHLD
                     G"
     gene            13774..14733
                     /locus_tag="VFB53_00325"
     CDS             13774..14733
                     /locus_tag="VFB53_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN85204.1"
                     /translation="MQEPTMLLRTIGGWFPAPMLATFAVFASLPFWIKGVGLYPYLGV
                     EVMIWVIYALGFNLLLGYGGLPSFGHGAFFGVGAYAFGLTQLHVAPSLWLCLAAAILA
                     GGLAGALVALFISHRRGIYYALLTIAFGQVFWFVAIKWHSVTRGEDGLLNIRRPAFLD
                     DLHALYWFCFAAFVLVVVLLWRLVNAPFGRVLSAIRQNETRARFVGYHVWLYKWLAFT
                     VSALVAGLAGGLFALAQQSAYPNVMSLHQSGFVVMMALVGGGLVSFWGPVIGAALFFL
                     ARDLLGAYTETWLLWYGLLFMAIVLFKPEGLAGMFHVRSKPAA"
     gene            14708..15424
                     /locus_tag="VFB53_00330"
     CDS             14708..15424
                     /locus_tag="VFB53_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85205.1"
                     /translation="MFEASRLHKRFGQHVVLEDVSLRFEAGRLSGIMGPNGAGKTTCF
                     NLLTGRYAPDRGKVTFEGRDITGLPPRAIARLGISRSFQVMNLFDDYSALDNLRVALP
                     ETRARGLSMLGAFGGETDVLARVGLAGKGGVKAKSLSYGDRRALEIGVALAARPRLLF
                     LDEPTAGLGTEGTARIGRLIAELKRSITIVMIEHDMKFLFGLADHITVMHWGQVIAQG
                     TPAMLRANPWVRRSNLGQLA"
     gene            15424..16119
                     /locus_tag="VFB53_00335"
     CDS             15424..16119
                     /locus_tag="VFB53_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85206.1"
                     /translation="MLALEGIHSFHGETEALFGVSLEVAAGEVVALLGPNGAGKTTTL
                     RSILNLTEVRRGAIRFDGADITRAPTHRIARRGIGWVPDDRRIFPTLTVERNLAIAKK
                     RSRFRAWTEQECFELFSALEYLRHRECENLSGGEMQMVAIARALVGSPGLVLFDEPSQ
                     GLAPKIVQDVMRAIGRLKAEGVAVLLVEQNPLAALGAADRVYVMDKGRIVHRGTARAL
                     LEDAALRTRLLGV"
     gene            16116..16727
                     /locus_tag="VFB53_00340"
     CDS             16116..16727
                     /locus_tag="VFB53_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85207.1"
                     /translation="MTLLAVDKVVKEYARGWPRRRVTFRLEASFSVEQPGIVGVMGPN
                     GSGKTTLFELITGSNAPTSGRVLCMGQDIHGVKARERDRLAIHYHQSYQVRRFRRRRP
                     DFLLEAAKSDTPMVHLFDEPQFNAQDGYVGFMLDFFRRLKGEGRAVFVCLHPTEPFHL
                     DILQEVCERFLYVERGRISQFPGYADALADARVAAYLGEGARR"
     gene            16724..18229
                     /locus_tag="VFB53_00345"
     CDS             16724..18229
                     /locus_tag="VFB53_00345"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN85208.1"
                     /translation="MNKPAELKLDTVPCWIGGRRVVPEGRMGEVFNPATGQVAKRVPY
                     CDAAAIDAAVQAAAAAYPEWRDSPVLRRARVLQKFLQLLQANQKEIARLITAEHGKTL
                     PDAMGSVQRGIEVVEFACGIPQLLKGEYSENVGTAVDTHTVRQPVGVCAGITPFNFPA
                     MVPLWMFPVAIACGNTFILKPSEKVPSASIRMAELFREAGLPDGVFNVVHGDKAAVDA
                     ILAHPGIAAVSFVGSTPIAKFIYETCAKNGKRVQALGGAKNHAVVLPDADLEFAADAL
                     IGAAYGSAGERCMAISAVVAVGAAADPLVRLLEKKAKAIKVGPGDAEGVEMGPLVTAQ
                     HRDKVASYIEQGKAEGAKAVLDGGAPPVKEGFFLGTTLFDHVKPEMRVYRDEIFGPVL
                     VVTRVATLDEAIALINRNPYANGTAIFTESGGAARRFENEVQVGMVGINVPIPVPVAF
                     YSFGGWKNSLFGDLHVHGAEGVKFYTRTKVVTTRWPHQDTPAPGLNMPTLG"
     gene            18245..19111
                     /locus_tag="VFB53_00350"
     CDS             18245..19111
                     /locus_tag="VFB53_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZN85209.1"
                     /translation="MEAILVTLILGTATPGGGFPVYGDAFADMVNAQDAKLVVKTTNT
                     KGSAENVPLLESGKLDLGLVAGELASAALAKPGTRLRIVAAMYSAPGMFAVRGDSPVR
                     AIADLKGRPVALGAQGSGLTVLGRGVLEALGIEVQPITLEKAADGPAMVLDGRAAALW
                     GAGVGWPGFTALAKAGGRFVAPSAAEIQQILAKNSALKALTMPAGSYPGQDAPVHTVG
                     SWSFVLARPELPDDVAYRLARAVHRAEAPFAARLAQARETTMANTAAAAPAPELIHPG
                     VRKYLREIGAEK"
     gene            complement(19128..19997)
                     /locus_tag="VFB53_00355"
     CDS             complement(19128..19997)
                     /locus_tag="VFB53_00355"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN85210.1"
                     /translation="MAFQVEVAARGADLLGECPLWDERGRLLWWVDSRWPAVKQLDPA
                     SGAVMMLVLPEVVGSIAFREQGGLLAATKSGIHFLDAASGALEARANPEAALPENRFN
                     DGRCDRAGRFWAGTMSDVKRDPVGSLYRFDADMACTRLRNAVVIPNSLAFSPDGGRMY
                     FADTMRHILWAYDYDPASGAATNERVFADAGAGRPDGSCVDAEGCLWNAEYGGWRLVR
                     YTPAGKVDRVVEVPVANPTCCCFGGEDLGTLYVTTARQRLSPEELARQPLAGSLLALR
                     PGVQGLPEGRFAG"
     gene            20033..21004
                     /locus_tag="VFB53_00360"
     CDS             20033..21004
                     /locus_tag="VFB53_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN85211.1"
                     /translation="MKVVFYGSLIHHLTDTLLAKVKTPWETARFLEKDPPEALARALA
                     QADALISLVWNRSFPAAPRLKLIQSPGTGVDAIDFAAVPPGVRVCNCYGHAEGTAEYV
                     LLSILLWCHVNFVPAHQSFRAERSWRYSGRVNGPINEELHGKTVGILGLGAIGSAVAA
                     RAKAFGTRVLGCNRTPRNVECVDKQYPLAQIDEFLAECDFVVIAAALAPETERLIDAG
                     RFARMKPSAVLVNVGRGAIVDEDALYAALKDRRIRGATLDVWYAYPRHDDLAVAPSRH
                     DFFALDNVWMTPHTSAWTTGMVERRWSEIAANLDALARGAPLRHQLR"
     gene            21001..22074
                     /locus_tag="VFB53_00365"
     CDS             21001..22074
                     /locus_tag="VFB53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN85212.1"
                     /translation="MKLERWSLQCYRLPYLREVVWANAREDAGVFALLKLEAAGCTGI
                     AEGTLKDTWSGASPKSLAAALEDFLIPRLRAADLADELAVARAFAGIPENRLAKGMIE
                     SACWTLRAAVAGAPLWKLWGAGRSREVAWTVTRQAPKAMAVEAVDMCAQYGFRTLKVK
                     GGQGVEVDLRALAEIQSAVGGGVELYVDANSAYAREDALEYVQGLASAGVTVAEDPCA
                     LQPDRHFEELQRASPIPILVDRSCASKEDAALYLERGAKALSTKPGRVGLAETRAIAA
                     LAAARGAKTAVGIYAESALGTLVNLQQPGTMAAEQTFFLAMKEQVSRVLPEIRGGRIE
                     LPAEADLAQLVDWGRLAQLAYNS"
     gene            22104..24068
                     /locus_tag="VFB53_00370"
     CDS             22104..24068
                     /locus_tag="VFB53_00370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZN85213.1"
                     /translation="MAKQQIYDASAIQALKGLEPVRRMPGMYTHTVHPLHIVQEAIDN
                     AVDEALAGFGKRLGVRVFKDGSVEVEDEGRGIPVDMHPEEKKPAVEVAFGMLHAGGKF
                     AKSGTGVYHISGGLHGVGVAVSNALSRRLEVTVYRDGGRHSIAFEGGELKNKLKSERI
                     KEKRSGTVVRLWPDPKYFDSPNIPLPELEHLVRSKAYLLPGLTTVLEVEGKEKKSWKY
                     ERGMAQYFEFMLEGRELAAPLFTGERYFAESSIPEIEPGEGASWAIGWVAEGEVFADS
                     HVNLIPTRSGGTHEAGFRAGVFDALGAFMEARALAPKGVKLIADDVWARACFTLSARI
                     VRTQFHGQTKEKLTTRHAAKLLELCVKDAFELWLNEHPEEGKRIAELAIEQALARLAK
                     GKKVERKKSSGIATLPGKLVDCASGDVSRNELFLVEGDSAGGSAKEARDKESQAILPL
                     RGKVLNTMSKDSRTVLANKELQAIATAIGVDPHGPEDAADLGGLRYGKVILMTDADVD
                     GGHIQALLLTFFFMHAPKLVEAGHVYVAQPPLFKVEIPAQGKGRPQRRAYCLDDDELD
                     ETLAALKKEKVKEGSWEISRFKGLGEMSPEQLWETTMNPDTRRLVRMQLDAKQLKKAR
                     ETFELLMDSGEAEGRRNWMREHWKTVEADI"
     gene            24079..24297
                     /locus_tag="VFB53_00375"
     CDS             24079..24297
                     /locus_tag="VFB53_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85214.1"
                     /translation="MAKKQKAFWERAYKGHVLWQGRQKLGKVTLAGEGRYTWEAGSRA
                     GASDDLEKAKKAVELAVAMADKQLDLFR"
     gene            24300..>25075
                     /locus_tag="VFB53_00380"
     CDS             24300..>25075
                     /locus_tag="VFB53_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZN85215.1"
                     /translation="MDTRTPDLFTPENGDDAAPIERHASQAYLGYAVSTVKSRALPEI
                     ADGLKPVQRRILYAMGDAATQGFAKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSM
                     RYPLIDGQGNFGSRDGDSAAAYRYTEAKLSRYAELMLAEIGEGTVDFIKNYDGKFEEP
                     VLLPARLPFGLLNGSFGIPVGFSTRIPSHNLREVAEAAAHVIKHPKASVKDVLAILPG
                     PDFPGGGQVISPPEEIRQAYEAGRGSLVLRCKWERENLARG"
BASE COUNT         3665 a         8829 c         8957 g         3624 t
ORIGIN      
        1 gtgacccctc gacgttgacc ctgcgcaagc ccggcgctag attggctgcg ccccattatc
       61 caacaatgag cgcgcgcagt cacaaggccg gactcctcgt tgccgtgacc ttgtccggca
      121 tggcggtgct gatcatcgag atcctcgcca cgcgcatgct ggcgcctttc ttcggcaatt
      181 cgatcttcac catctcgagc gtcatcggcg tcgtgctggc ggcgctgggc ataggctact
      241 acctcggcgg cgcgctggcg gagcgccggc cctcggcggc gtggttcttc tcgctgatcg
      301 tggccgcggg gttcgcggtg ctgctgctgc aattgctcaa cgccgccctg ctgccgggcc
      361 tcgcctaccg gctgtccctg gtcaacggcc cgctgctggt gtccctgctg atgttcttcc
      421 tgccggccct gttcctggcg atgctctcgc cgttcgccgt cgcgctgctg catgcgcgcg
      481 aaccgggccg cggcgccggc catgcggccg ggctggtgtt cttctggtcg acgctgggca
      541 gcatcgcggg cagcctcggc acgggcttcc tgctggttcc ccgctggggc atcggcagca
      601 tcgtcctcgg cgtgggcgcg ggcctggtcc tgctcggcgg cgccgggttg ctggcgacgc
      661 gaggcgtgcc gcgcgtcctg cccgccggcc tggccctgct ggcgctcgcc tgcggcgccg
      721 ccctgcggca ggtgccggcc gacgaaggcc gcggcgtggt ctacgccgcc gacggactgt
      781 acgagcgcat cgtgatccgc gagctgcccc tgcgcggccg caccgcccgc gtcctgctgc
      841 aggaccgcaa cgtcagcggc ggcctgtacc tggacgacgg cagcatggcc ttcgactaca
      901 ccaagtacta cgagctgtac cggctgttca cgccggaatt gacggcggcg ctggcgatcg
      961 gcggcggcgc gtacaacgtg cccgccgccc tcctgcgcgg cgcgccgcgc gcgatcgtcg
     1021 acgtggcgga gaccgatccg tcgctgcacg cgctggcgct gcgctatttc gcgctgcccg
     1081 acgacgcgcg gctgaggaac cacgtgatcg acgggcgccg cttcctgcac gacaccgagg
     1141 agcgctacga cctggtgttc gccgacgcct ttcgcgactt cgtttccatg ccggcgcagt
     1201 tcgccagccg cgagttcttc agcctggcga ggagccggct gcgcgaggac ggcgtgctga
     1261 tcgccaacta ctacggcagc ctggcgccgg attcccgcgc catgctgtac tcgatgctca
     1321 ggaccatgcg cgccgcgttt ccgcaggtct acctcctcgc gacgctcggt ccgcagagcg
     1381 aggagctgca gaatttcatc ttcatcggcc acaacgcctc gcccgcggag aggcgcatcg
     1441 acctgcggca ggcggccgcc gtcgcgttcg cccacccgga gctgaagcag gtcgccgcgc
     1501 tggaactgcg tccggcggaa gcgctactcg aggcgcatgc cgtgctgacg gacgatttcg
     1561 cgccggtcga gtaccacgcg gcgggcgcga tccgccgcta cgacgctaca ggtcgacgcg
     1621 cagcatcatc gagaagccgt actccctgac ctggtcctgg cccacgcgct ggctgaacac
     1681 gaagttcgcc gtcaggccct tgccgaagtg ccgccagccg gcggtgagca gggtgctgtc
     1741 gtcgtcgttc gccggctgcg cgccggtcgc gaactggacc tgcagcgggt cccgggtctt
     1801 ctcgtacagc gcgccgaggt agaactcgga cgcgccgcgg ccgtaggcgg cctccgccag
     1861 caggttcccc tgctcgacgc tctggctgga ggcggctacc gcggtgccgt cgctttccgt
     1921 gtaggcgtcg tgcttgcgct tcgccgccag caggccgagc gccccggtca gcgtccagcc
     1981 cccccaggtc gagacattgg tcaggttcac cgaggcgaat tcgcgcgtcg agtcgaactt
     2041 gctgtccacg gggaccgaaa ccagcggcgc cagtcccacg gtcctcgact gccgcgtgtc
     2101 gaattcgccg tagccggcgc tcgcgtccag cgtccacgcc ggggtcagca ggtatgaaaa
     2161 atagggggcg aggttgaagc cgcgcgtcct ttcgttgccg gcgttgaagg tggtggtgaa
     2221 attgctcgcc tcgtggccgg cggcgacgcc gaacacgaat ttttccgacc gggtcatgtc
     2281 gaagccggcc agcaggttgt gggtggcgcc gtagaaggcg gtgcgcgaga aggtattttc
     2341 cagcgagttg gcggcagcgc tgatccacag gctcgcggaa tcgccgccgc cgcccgccgc
     2401 ggacggctcc ttcaggccct gcgccagcag gctgaaatcg ccgcggctgt ccgcctgcgc
     2461 gctggccacc agcacgcggc ccgcctgcgc ctggccgcgc gagcctttcg cgcggcgcag
     2521 gccgagcaat tgcgcccgga tggcgcccgc gatcggcgcc gcctgccgct cgacggtcgt
     2581 cgcttcggtg atggtgttgc tggggctggt ctgctcgtcc ggcggcagga tcagcgtgaa
     2641 gggcagcgcg gcgccggcgt acaggaatag cgcacttgcg agaaaccgac tgagggcctt
     2701 ctgcagcacc gagttcccct aggaggggcc cagcatagtg caagaccggg acggtgacaa
     2761 cgcttacgcg gcgccgatgc gcccgggcgc cggccggtgg ccgatgccgc gcgagatgcg
     2821 ctcggcggtc tccttgatga gctgccccca ctgcggcttc atccggtcgg cgggggtgga
     2881 cagcgacagc gcggcgatca gctggccgtt gtcgtcgcgc acgccggcgg cgacgcagcg
     2941 cacgccgagc tcggcttcct cgttgtcggt ggcccagccc tggcgctgcg tgcgctccag
     3001 gtcgcgctcc aggagctgca ggttgccgag cgtgttcctg gtatgcgccg cgaggccggt
     3061 gcgcttggcg tactcgcgca gccgcgcgaa gccgtcctcc agcaggaaca gcttccctgc
     3121 cgcggtgatg tgcagcgcgg cgcgcgcgcc gatcacgtgc accacgcgca tcgccgtgcg
     3181 gcccgaggag gtgcgctcga cgtacacgat ctcgtcgtcg tggcgcaccg agaggttcac
     3241 cgtctcgccg gtgtgcgcgt gcagctcgcg catcaccggc agcgcgagtt ccctcacgct
     3301 gatgccggag cgcaccacgt tgcccagctc cagcaggcgc atgcccaggc ggtagctgcc
     3361 gggctcgacg cggtccacca gccgcccgcc cgcgagggcc gagaggatgc ggtgcgcggt
     3421 ggaggggtgc agcgcggtgt actgcgcgat ctgcttcagg ccgagcggct cggggtgcgc
     3481 ggcgagcacg tcgagcagct tcatcatgcg gtcgatgacc tggaccgggc ccttcgcgtc
     3541 cgccttcgcg ctcatggtcg cgcaattcta tcccacgatg cgaaacgcgc gcgcctgctg
     3601 ctaccatcgg catggatgcc ggtggtgacc aacatcgagg acctgcgccg catggcgcaa
     3661 aagcgcgtgg cgaaggcgat cttcgactac gtggaccgcg gcgcctacga cgagcagacg
     3721 ctgcgcgcca accgcgccga cctggaggcg ctgcatttcc gccagcgcgt ggcgatcgac
     3781 gtcgaccggc gctcgacgcg cggccgcatg ctcggcgagg aggtggcgat gccggtggcg
     3841 atcgcgccgg tgggcctctc gggcctgaac tgggccgacg gcgagatcct cggcgcgcgc
     3901 gccgcgcaag cgttcggcgt gcccttcacg ctgtccacca tgtcgatctg ctccatcgag
     3961 gacgtggcgg gcgcggtggc ccggcccttc tggttccagc tctacgtgat gcgcgaccgc
     4021 ggcttcgccg cctcgctcat cgagcgcgcg aaggccgccg ggtgcacggc aatcgtgctc
     4081 accctcgacc tgcagatcca gggccagagg cacatggacc tgaagaacgg cctcgccgtg
     4141 ccgccgcagc tgacgctcgg cacgctgctc gacgtgatgc ggaaacccgg ctgggcgctc
     4201 aacgtgctct ccggccggcg caagtccttc ggcaacctcg ccggccgcat cccggacgcg
     4261 aacagcctga ccacgctctc gcagtggatc gcggggcagt tcgacccgac gctgtcgtgg
     4321 aaggacgtgg agtgggtgaa gggcctgtgg ggccgcaagc tcgtcctcaa gggaatcctg
     4381 gatgccgagg acgcgaagac cgccgcctcg agcggggcgg acgcgatcgt ggtgtccaac
     4441 cacggcgggc ggcagctcga cggcacggtg tcctcgatcc gcgcgctgcc cgaggtggtg
     4501 caggcggtgg gcggcaggac cgaggtgtgg atggacggcg gcatccgctc cgggcaggac
     4561 gtgctgcgcg cgctcgcgct cggcgcgacg ggcaccatga tcggcaagtc ctacgcctac
     4621 ggcctgggcg cgatgggcga ggcgggcgtg acgaaggcgc tcgagatcat ccagaaggag
     4681 ctcgacgtca ccatggccct gtgcggcctg cgcgacgtca aggacgcgag cccggcgatc
     4741 ctgcgcggcg cctagcgcgg gccgcgaagc ggccgggcgc gagcgcctcg gcgagcgcgc
     4801 cttccagcgg caagggctcg ccttccatca ggctcgccag caattcgccg cccaatcccg
     4861 cccacagcag gccgcgcgag ccgtaggcga aggccgcgta caggccgccc gtctcttcca
     4921 gcccgccgat gaccggcagg cggtccgccg ccaccgcgcg aaacgaagtc cggccctgca
     4981 gcgtcgctgc gtccagcccg ccgctcgcgc ccggcaggat gcgctccagg cgctcgaggt
     5041 tgccggcatg gctctcggcg cgcaccgagg cgtccgcgtc ctccaggtcg aagctcgccc
     5101 cggtcaccga taccccgtcg atggcgggca gcagcatgcc gccgcgcagc agcacgcagc
     5161 gcagcgcctc gatcggcggc aggtgggtga gctggccgcg cacgcggcgc agggcgatgt
     5221 gcttcgcggg cgcgaggcgc agcgcgtcgg cggcattcgc cagcaccacc gcgccggcct
     5281 cggcgatcgc ctcgccctcg ctgtcgcgca ggatccagcg ctccccggcg cgctccagcg
     5341 ccgccgcctc gcggccgtag caggcgcgcg cgcccgagcg ctcgatgagc gcgcgcgcga
     5401 agctgcgcgg ccggatccag ccgccctcgg ggaaccacaa tccgcccgcg gcaagccgca
     5461 cgcccgcgca ctcgctcgcc cgcgcgcggt ccaggcgctg cgcgaaggcc tccgggaagc
     5521 cgagcgcggc gagcgccgcc gcctgcgagg cgtcctcgcg cgcatcgcgc gcgagctgca
     5581 gcacgccgca gcgccgccac tccggcgccg cgccgatggc gtcgaactgg cgccagtgct
     5641 ccagcaggaa gctgaacgcg gcgcgcgtga ggcgcgcgat gtggctgtcg tccgggctga
     5701 ccacgggatg gaaggcgccg gcgtggttgc ccgaggcttc cagcgccggc tcgaggtggc
     5761 gctcgacgac cgtgacttcc cagccgcgcg cggcgaggcg ctcggccgcg gcggcgccgg
     5821 cgatgcccgc gcctaccacg atcgcgctgc gctccgcggg ccgtgcgggc gcggtgcgga
     5881 ggctccgcag cggcacgaag cgcgccacca gcatctcgct cttcctcgcg tagccgcggc
     5941 gcttctcggc gacgaagccc gcggcgcgca ggccctcgcg caccggcgcg gcgacgctcc
     6001 aggtggcggc ggtggccccg ggcgcggcga ggcgcgaaag cgcgcgcagc aactccggcg
     6061 accacagttc cgggttcttc gccggcgcga agccgtcgag gaagaaggcg tcggcggcga
     6121 ggcgcagctg cggcaccagc gcctgcgcgt cgccgaaggc gagcgtcagc accacgcggc
     6181 cctcctcgaa ttccagccgg tgcaggcccg gcaccagcag cggccaggcg ctcctcagct
     6241 cctcggcgag cgcggcgtac tccgggtgcg gcgcgtgcag cacggcgagg tcctgcgccg
     6301 tgaacgggtg cttctccacc gacacgaagt gcagccgctc gcagcgccgc gcatcgtcgc
     6361 gccaggcctg ccaggcggcg aggaagttga gccccaggcc gaagccggtc tcgaggatgg
     6421 tgaagcggag cgcgccccgc cagcgcgcgg gcaggccgtt gccgccgagg aacacgtgcc
     6481 gcgcctgccc cgggccgccg tcggcgctgt ggtacaggtc gccgtaggcc tcggagtacg
     6541 gcgtgccgtc ggcggcgaag gcgaggcggg cgggggcgag cggggcgggg ggcaccccgc
     6601 tattgtgacg cggtgatgcc gccgtcgagg tacaggatct gcccggtgac gtaatccgac
     6661 gcgctcgagc agaagaacag cgccgcgccg acgcagtccg ccggctcggc gatgcgcccc
     6721 agcgggatgc gctccttgag ccaggcgcgc gtcttcgggt tcttcagcca gtgcgcgccc
     6781 atcttcgtgc gcaccacggt gggcgcgatg ccgttcacgc agatgccgcg ccggcccagt
     6841 tccgaggcgt gctggcggat gagcatggcg aggccgccct tggtcgagca gtaggccgag
     6901 tagccgcgcc cgcgcagccc gagctgcgcg cgcaccgaca ggaggtgtac ctgcttgccg
     6961 ccgcgcttct ggcgctttgc aaccgcctgg gcgaggaaca tcgccgcgcg caggttggtg
     7021 cggtagatcg cgtcgaaggc ctcctccgtc acgtccgcca ggagctgctc gcgctgcatg
     7081 ccgacgcagt tcaccaggat gtcgatgcgc ccgagcgagg cgaccacgcg gccgatggcg
     7141 gcgacgtcct cgacgtcgag cgccacgccg cgcgcgccgc ggccgagctt tttcgccagc
     7201 gccgcggcct tcccggcgtc gcggccggca acgatgacgc gtgcgcccgc ccgcgccagg
     7261 ccgcggcaga tcgcgccgcc gatggcgccg tagccgcccg gcaccagcgc cgtcctgccc
     7321 tggagcccga acaggtccat cctcaatcca tcaccacgcc gccgttgagg tccacgatct
     7381 cgccggtgat gaagccggcg agcgggtgca cgaggaaccg caccacgtgc gcgaactcct
     7441 cgggctcgca aaagcgcccc accgggatct gcgcgagcac cgccttgcgc tgcgccgcgg
     7501 agagctgctc ggtcaccatc ggcgtattga cgtaggcggg cgagatgccg ttcacggtga
     7561 cgccgaaggg cgccagctcg cgcgcgagcg aaaaagtgag cgaggtgagc gcgcccttgg
     7621 aagcggagta ggccgtgccc gcggtgaggc cgccggtctt ggcggcgagc gagcaggtgt
     7681 tgacgatgcg gccccagcgg cgctgtttca ttcccgggat caccgcgcgc gacaggtaga
     7741 acgcgccgtc caggttggcg gccagcaccc ggcgccactc ctcgggcgcg gtgtcctgcg
     7801 acttgttgtt cgacaggatg ccggcgttgt tcaccagcac gtccacgcgg ccgatgccgc
     7861 gcaccgcgcg cgccaccgcc tgcgggtcgg aaatgtcgca gaccacgccg ccctggtgcg
     7921 ccttcacgtc gatgccgacg acccgcaggc cgtccgcggc gagcgcccgc accacggcgc
     7981 ggcccatggt tcccgccgcg ccggtgacga cggcggtcct gcccctcagt tcagttttca
     8041 agcgcggtct tcttgccgaa gagtccccgg gcccggaagg ttaccagcgc gatgagcagc
     8101 aggtacatgg agagcagcga ccactccgag gcgtaggcgc cggtgaggcc gaccacgatg
     8161 cccaccagga tgccggcgag caccgcgccc cagaagctgc ccaccccgcc cagcacgatg
     8221 atgaggaagg cgggcaccac ggcgtccacg cccatgtgcg gccgcacccc ccagatcggc
     8281 gcgagcacga tccccgccac cgcggcgagc gcggtgccga gcgcgaacac cgccaggcgc
     8341 agccgcggca ggttgatgcc cagcgcgcgc accatctcgg aatcgtgcgc gcccgccttg
     8401 atcatcgcgc cgtagggcgt cttctccagg aacagccaca gcagcaggat caggccgacg
     8461 gccgccgcgc aggcgaacat gcggtacttt gcgtacagca ggccgccgac cagcgtcgcg
     8521 ccggagaaca tctccggcac cggcagctgc ttctcgttcg agccccacag cacgcggatc
     8581 agctcctcga tcaccagcgc cgcgccgaag gtgagcagca ggccgtacag cgggtccttg
     8641 ccgtaggtgc ggcgcaggca cagctccagc agcatgccga acgcccccac cagcagcggc
     8701 gccagcagga acgccgcggc atagctcagc ttccagccca tcagcgtgat cgccacgtag
     8761 gcgcccagcg cgaacagcga gccgtgcgcc aggttgatca cctccatcac ccccacgatg
     8821 agcatgaacc ccagggcgat cagcgcgaac agcagcccga ggctcacccc gttgaggagg
     8881 tgagggagga ggttgagcag gacgtccaag ccctactgct cgtacgaggg cgtggccgcg
     8941 tagctctcca gcttgcaggc cgcgggcgcg ccctggtcga cgacgtcctg cggcgccacc
     9001 tgcgcctgga tcctgaagat gtcgtccttc tccgccggct tgtcgttgta gctcgccatg
     9061 tagatggtct gctgcacctg gtgcgtgctg gcgtcgatcc acgcgtcgtg gtgctgcatg
     9121 cggtccgccg cgctcatctt gcggccctcg agcttcttga tcaccgcgag gttgttggtg
     9181 ctgcccgcct cctcgacgca gcgcagcagc tcgcgcgtgg cgacgtagcc gttgtagaac
     9241 acgttgcccg gcaccgcgat ctggatgccc gggtaggtct tctggtacct ggcgacgaag
     9301 tccttcacgc cgggcaggtc gaggcggtag taccaggtgg tgccgaacac gccgaacacc
     9361 gcgtcgggcc ccaggccgta gacgtcgggc cagtcctgct ggttgttgat ccaggcgaag
     9421 gacttgtcga gtttcgtctg caccacctgc tgcctgagcg ccttgatgtc gtcgccgccc
     9481 accgcggccg cgatcacgtt cggcttgatc tgctggatct tgagcaggta ggacgtgaag
     9541 tcgcgcgtgt tctgcggcac catcagctcg tccacgatct tgccgccgtt cgcctccacc
     9601 agcgtgcgcg tcgccttcga ggtgttgtgg ccccagacgt agtcgttggt gagcagcagc
     9661 cagttgcgcc cgctggcgcg catggcgttc ttcacgatgg catgcgcgaa gttggtgccg
     9721 ttgccgtccc atacgaactt cacgcggtgg cagtcgcgcc ccgcctcggt gggcgagctg
     9781 gagttggtgt tgaagaacac gcagccgtac ttctgcgcca cctggctgat ggcgttggcc
     9841 acgcccgagt gcaccgcgcc gatgaggaac gcggcgtcgt tgcgcgtgat catgcgctcg
     9901 gcgacgcgcg agccggtggc cggcgtggtc tccgtgtcca tgtgcagcgc ctcgatctgc
     9961 cgccccagca cgccgccctt ctggttgaat tcgcggatgg cgagcatcgc gcccaggcgc
    10021 tcgtccatgc cgctggcgcc gtactggccg ctcgcgtcca tggtgaggcc gatcaccagg
    10081 ggcttcttct gcccggcctg cgcccaggcg cggttcacct tccacgggcc gtagaacgcc
    10141 gcgcccgcgg ccagcatctg cagcaacttc ctccgattgc cttgcatgac tccctccttc
    10201 agtgtttgcc tacgcggatc ccgttcacct tctcatgcgc ctcgatgagt gacgggacga
    10261 acttgtcgcc gaggccgagc gcgttcgcct gcaccagcga ttgcatcagc gccgcgccca
    10321 tgctgccggc gaacggcgcg ttggccacca tgccctggta gtagccgatg tccttcctgg
    10381 cgttggccag ggtgaacagc agcccgccgt ggtcgccctg cagggccttg ggcacgatca
    10441 tctggaacat cgcggagttg gcgccgccgg cggagatcag cttgtagaag gtctccaggt
    10501 cgatgccgac cttggcgcag gcggcgagcg cttcgcacac cagcgccgcg gtgcccaggg
    10561 cgaggaagtt gttcaccagc ttcaccttgt gccccgcgcc ggggccgccg acgtggaaga
    10621 tgttctcgca gaacttctcc agcaccgggc ggatcgcggc cagcgtcttc tcgtcggcgc
    10681 cgaccatgca gttcagcttg ccttcctcgg cctgcaccgg cgtgcgcgcc agcggcgcgt
    10741 cgacgtaggg cacgcccttt ttcgcgaagt cggcgcggat gcgctcagtg gattcgggga
    10801 ggctggtcga gcagtcgatc accacctggc cctggcgcgc ggcctccagc agcccgccgt
    10861 agacgttggc ctccacctgc ggcgtgccgg tcacgcagag gatcacgacg tcggattcgc
    10921 gcgcgatctc ggcgctcgac ttgagctccc gggcgccgag ctcgagcagg tcgccgagcc
    10981 tggcgcggtt gcggtgcgcg aggaaggcga gcggatggcc cttcgccaga aggttcttcg
    11041 ccatgccgtg gcccatcagg ccgatgccga tgaaaccgat tctttgcata aggtcctttg
    11101 cttgcgccag agcccggcca gccgggcgta gtttgccgcc acctcgtcca ccacctgcgc
    11161 gtcgccgcag gcgccgcgga accagcgccg cgccgcgtcc atgaagatcg agcgcccgac
    11221 ggcgaagccc ttgcacaccg ggtggcgcgc ggcgagcgcg aagccgcgct ccagttcctg
    11281 ctcgtccgcc tccatgccga gcagcagcac gccgcggcag tgcgggtcgt gccgcgcgat
    11341 gctgccggcg atggcgcgcc actccgcgtc gctcgccggc ggggcgagct tccaccagtc
    11401 cggcttcacg ccggcggcgt agatctcggc gagcggcggg ttcctcgaca ggagctcgag
    11461 cagcagctcg tgcccggtgt ccacgcaggc cgcgtagagc tgccgcagcg ccggcaggtc
    11521 ccgcggctcg tagtgcacca ggcacttcac cacgtgctgc gcgggccagc cgcgcagcgc
    11581 caccccgacg ttggcgccgg cttcgaaggc gagcggccgg ctgccgggcg cctccaccgg
    11641 ccgcgccacc cagaggccgg agccggcgag ggcgaacagc gcctcctcgc cgtgccggtc
    11701 gtcgacgatc gcgccggcgc ccggcacgcg cgcgcgcacc agcccctcgg ccaccaggcg
    11761 cttgaagcgg cggatgcgct tgggcggccc gagctcctcg agctgcgtgc ggtggtcgaa
    11821 ggcgagcgcc atcacctccg gccagggcct gcggccggtg gtgacgcggt gcaggtggtt
    11881 gagctcgggc gattcgcgca gccggtgcgg ccagtcccgg cgcgagagga acaaggccag
    11941 ttcctcctgc gagggcatgg cgggcgcgca gccgtggcgc gacaccacca gcgcgccgca
    12001 ggcgttggcg cgccgcgcgc attcgccggg cggcaggtcg cgcagccagc ccgagaggaa
    12061 gccggcggcg aagccgtcgc ccgcgcccag cacgttgaac acctcgaccg ggaagccctg
    12121 gccctccagg gactgctcgc ccgcgtgcac gctgcagccc agcggcccgc gcttgacgac
    12181 gaaggtcgcc gcggagatcc ggcgcaggcg gcgcagcgcc tcgtccgtgt cctcgcagcc
    12241 gccggcgatg tggaactcct cctcggtgcc gatcaccagc tgcgcgagcg ggatcacctc
    12301 ctgcagcttg gccgtcacgt gctgcgaggc gacgaagcgc tgctcgccca tcccggggga
    12361 ggcgaggccc cacagcaccg ggcggtagtc gatgtcgaag acgaagcgcc tcgcctggcg
    12421 gatcgcgcgc aggcagacgg cggcggtctg cggctgcgag aaatgcgtgc ccgagacgag
    12481 cagcgcctgc gccgagccga tgaaggcggg gtcgaaatcc tccgccgcga tcgccatgtc
    12541 ggcgcagtcg tggcggtaga acaccagcgg gaaggtgtcg cggtccctga tggcgaggaa
    12601 cacgagggcg gtgagccgcc gcgcatcggt ggccacgtgc gacacgtcca cgccctcggc
    12661 ggcgagcgtc tcgcgcacga agcggccatt gtgctcgtcg cccacccgcg tcagcatcgc
    12721 caccttgagc ccgaggcgcg cggcgcccac ggcgatgttg gccggacagc cgcccaggta
    12781 cttggcgaag ctcgcgacat cctccagccg cgcgccgacc tgctcgccgt acaggtccac
    12841 cgcggcgcgg cccatgcaga tcaggtcgag gggccgctca gccatcgagg tgctccccca
    12901 gcgccaccac cagcgcctgc gccaggcaca tcggcgccac cagcatgcgg aacggccgcg
    12961 aggtcttctc ctcgatctgg aagcacaccg tggcggcgcg cgcgagcggc gacagggcgt
    13021 tgtcggtgat cgccaccacc ggcaccttgc ggccccggca ggcctgcgcc acctcgacca
    13081 cttcgggcgt gtagttcttg aaggacacca ccagcagcgc gtccttggcg ccgatcaggc
    13141 gcgccggctc cttcagcatg ccgcccaccg cgtcgagcag cggcgcgcgc agctccagct
    13201 gcgtgagcgc gtaggacagg tagtaggcga cggggaagga gcgccggtgc cccagcacgt
    13261 agaccgactg cgcggccgcc agcagcgtca ccgccgcctg caggtccgcg ccgctcacgt
    13321 gctcgtgcag ctgctcgagc gaggcgatgc cgtcgtcgat gaattcgtcc agcacggcga
    13381 ccgggccgga cttggcgtgc gccgcgcgga aggcctcgat gcgctcgcgg tagttgcgca
    13441 ggccggggga gtggttggag cgctccacca gccgcgtgcg gaatacgcgc tgcaggtcgg
    13501 agaagccgcc gtagccgagc gcctgcgcga agcgcaccat ggcggagggc tgcaccccgg
    13561 cgcccttggc gaggcgcgcc accgtgtcga gcgccatctc ctgcgggttc tccagcgcgt
    13621 aggtggcgat cgcctgcaat tgcggcgaca gccccgggta gcggcgggac aggtccgtct
    13681 tcagcgactc gaagctgcgt gcgcccggca taatcctctg gacaggcccg tctagaacat
    13741 ttattctcaa atcgtacata acgagaacaa gcgttgcaag agccgaccat gctgctgcgc
    13801 accataggag gctggttccc ggcgccgatg ctggcgacct tcgccgtgtt cgccagcctg
    13861 cccttctgga tcaagggggt ggggctgtac ccctacctcg gcgtcgaggt gatgatctgg
    13921 gtgatctacg ccctgggctt caacctgctg ctcggctacg gcgggctgcc ctccttcggc
    13981 cacggcgcgt tcttcggcgt cggcgcctac gccttcggcc tcacccagct ccacgtcgcg
    14041 cccagcctgt ggctgtgcct ggccgcggcg atcctcgccg gcgggctcgc cggggcgctg
    14101 gtggcgctgt tcatctcgca ccggcgcggc atctactacg cgctgctcac catcgccttc
    14161 ggccaggtgt tctggttcgt ggcgatcaag tggcacagcg tgacgcgcgg cgaggacggc
    14221 ctgctcaaca tcaggcggcc ggcgttcctc gacgacctgc atgccctgta ctggttctgc
    14281 ttcgcggcct tcgtgctggt ggtggtcctg ctgtggcgcc tggtcaacgc gcccttcggc
    14341 cgggtgctct cggcgatccg ccagaacgag acgcgcgcgc gcttcgtcgg ctaccacgtg
    14401 tggctctaca agtggctcgc cttcacggtc tcggcgctgg tcgccggcct ggccggcggc
    14461 ctgttcgcgc tggcgcagca gtcggcctat ccgaacgtga tgagcctgca ccagtcgggc
    14521 ttcgtggtga tgatggcgct ggtgggcggg ggcctggtgt cgttctgggg gccggtgatc
    14581 ggcgcggcgc tgttcttcct cgcgcgcgac ctgctcggcg cctacaccga gacctggctg
    14641 ctgtggtacg gcctgctgtt catggcgatc gtgctgttca aacccgaggg cctcgcgggg
    14701 atgttccatg ttcgaagcaa gccggctgca taagcgcttc ggccagcacg tggtgctgga
    14761 ggacgtgagc ctgcgcttcg aggcgggcag gctctcgggc atcatgggcc ccaacggcgc
    14821 gggcaagacc acctgcttca acctgctcac cgggcgctac gcgcccgacc gcggcaaggt
    14881 gacgttcgag gggcgcgaca tcaccggcct gccgccgcgc gccatcgcgc gcctgggcat
    14941 ctcgcgctcc ttccaggtga tgaacctgtt cgacgactac agcgcgctgg acaacctgcg
    15001 cgtggcgctg ccggagacgc gcgcgcgcgg cctgtcgatg ctgggcgcgt tcggcgggga
    15061 aaccgacgtg ctggcgcgcg tgggcctcgc cggcaagggc ggtgtgaagg cgaaatcgct
    15121 gtcctacggc gaccggcgcg cgctggagat cggcgtggcg ctcgcggcgc ggccgcggct
    15181 gctgttcctc gacgagccga ccgcgggcct gggcaccgaa ggcacggcgc gcatcggcag
    15241 gctgatcgcg gagctcaagc gctccatcac catcgtcatg atcgagcacg acatgaaatt
    15301 cctcttcggc ctcgccgacc acatcaccgt catgcactgg gggcaggtga tcgcgcaggg
    15361 cacgcccgcg atgctgcgcg ccaacccctg ggtgcggcgc tccaacctgg ggcagctcgc
    15421 ctgatgctcg cgctcgaggg catccacagc ttccacggcg agaccgaggc cctgttcggc
    15481 gtgtcgctgg aggtcgccgc gggcgaggtg gtggcgctgc tcggccccaa cggcgcgggc
    15541 aagaccacca cgctgcgctc gatcctcaac ctcaccgagg tgcggcgcgg cgccatccgc
    15601 ttcgacggcg ccgacatcac gcgcgcgccc acgcatcgca tcgcccgccg cggcatcggc
    15661 tgggtgcccg acgaccggcg catcttcccc acgctcaccg tggagaggaa cctcgccatc
    15721 gcgaagaaac gcagccgctt ccgggcctgg acggagcagg aatgcttcga gctgttcagc
    15781 gcgctcgagt acctcaggca ccgcgagtgc gagaacctct ccggcggcga gatgcagatg
    15841 gtggcgatcg cgcgcgccct ggtcggctcg cccggcctgg tgctgttcga cgagccgagc
    15901 cagggcctgg cgccgaagat cgtgcaggac gtgatgcgcg ccatcgggcg cctgaaggcc
    15961 gagggcgtgg cggtgctgct ggtggagcag aacccgctcg ccgcgctggg cgccgccgat
    16021 cgcgtgtacg tcatggacaa gggccgcatc gtgcaccggg gcacggcgcg cgcgctgctc
    16081 gaggacgcgg cgttgaggac gaggctgctc ggcgtatgac cctgctcgcc gtcgacaagg
    16141 tggtgaagga gtacgcgcgc ggctggccgc ggcgccgggt gacgttccgc ctggaggcct
    16201 cgttcagcgt cgagcagccc gggatcgtcg gcgtgatggg ccccaacggc tcgggcaaga
    16261 cgaccctctt cgagctgatc accggctcga acgcgcccac ctcggggcgc gtgctgtgca
    16321 tggggcagga catccacggc gtgaaggcgc gcgagcggga tcgcctcgcc atccactacc
    16381 accagtccta ccaggtgcgg cgcttccgcc ggcgccggcc cgatttcctg ctcgaggcgg
    16441 cgaagagcga cactccgatg gtgcacctct tcgacgagcc gcagttcaac gcgcaggacg
    16501 gctacgtcgg cttcatgctg gatttcttcc ggcgcctgaa gggcgagggg cgcgcggtgt
    16561 tcgtctgcct gcacccgacc gagccgttcc acctggacat cctgcaggag gtctgcgagc
    16621 gcttcctcta cgtggagcgg ggtagaattt cccagtttcc cgggtacgcc gacgcgctgg
    16681 ccgatgcgcg cgtcgccgcc tacctcggcg aaggagcccg cagatgaaca agcccgcaga
    16741 actgaagctc gacaccgtcc cctgctggat cgggggcagg cgcgtggtgc ccgagggcag
    16801 gatgggcgag gtgttcaacc ccgccaccgg ccaggtggcg aagcgcgtgc cctattgcga
    16861 tgccgcggcg atcgacgcgg cggtgcaggc cgcggccgcg gcgtatccgg aatggcgcga
    16921 cagcccggtg ctgcgccgcg cgcgcgtgct gcagaagttc ctgcagctcc tgcaggcgaa
    16981 ccagaaggag atcgcgcgcc tcatcaccgc ggagcacggc aagacgctgc ccgacgccat
    17041 gggctcggtg cagcgcggca tcgaggtggt ggagttcgcc tgcggcatcc cgcagctgct
    17101 gaagggcgag tactcggaga acgtcggcac cgcggtggac acgcacaccg tgcgccagcc
    17161 ggtgggcgtg tgcgccggca tcacgccgtt caatttcccg gcgatggtgc cgctgtggat
    17221 gttcccggtg gccatcgcct gcggcaacac gttcatcctc aagccctcgg agaaggtgcc
    17281 ctcggcctcg atccgcatgg ccgagctgtt ccgggaagcg ggcctgcccg acggcgtgtt
    17341 caacgtggtg cacggcgaca aggcggcggt ggacgcgatc ctcgcgcacc cgggcatcgc
    17401 ggcggtgagc ttcgtcggct cgacgccgat cgcgaaattc atctacgaaa cctgcgcgaa
    17461 gaacggcaag cgcgtgcagg cgctcggcgg cgcgaagaac cacgcggtgg tgctgccgga
    17521 tgcggacctc gaattcgccg ccgacgcgct gatcggcgcg gcctacggct cggcgggcga
    17581 gcgctgcatg gcgatctccg cggtggtggc ggtgggcgcg gcggccgatc cgctggtgag
    17641 gctgctggag aagaaggcga aggcgatcaa ggtcgggccg ggcgacgccg aaggcgtgga
    17701 gatgggcccg ctggtcaccg cccagcaccg cgacaaggtg gcctcctaca tcgagcaggg
    17761 caaggcggaa ggcgcgaagg ccgtgctcga cggcggcgcg ccgccggtga aggaaggatt
    17821 tttcctcggc accacgctgt tcgaccacgt gaagccggag atgcgcgtgt accgggacga
    17881 gatcttcggg ccggtgctgg tggtcacgcg cgtcgcgacg ctcgacgagg cgatcgcgct
    17941 catcaaccgc aatccctacg ccaacggcac ggcgatcttc accgagtcgg gcggcgccgc
    18001 gcggcgcttc gagaacgagg tccaggtcgg catggtcggc atcaacgtgc cgatcccggt
    18061 gccggtggcg ttctactcct tcggcggctg gaagaactcg ctgttcggcg acctgcacgt
    18121 gcacggcgcc gagggcgtca agttctacac gcgcaccaag gtggtgacga cgcgctggcc
    18181 ccaccaggac accccggcgc ccggcctgaa catgccgacg ctgggctaag ctcgggagca
    18241 aaccatggaa gcgatcctcg tgaccctcat cctcggcact gccacccccg gcggcggatt
    18301 ccctgtctac ggcgacgcgt tcgccgacat ggtgaacgca caggacgcga agctggtggt
    18361 caagacgacg aacaccaagg gcagcgcgga gaacgtgccg ctgctggagt ccgggaagct
    18421 cgacctcggc ctggtggcgg gcgagctcgc cagcgcggcg ctcgccaagc cgggcacccg
    18481 gctgcgcatc gtcgccgcga tgtactccgc gcccggcatg ttcgcggtgc gcggcgacag
    18541 cccggtgcgc gccatcgccg acctgaaggg ccggccggtg gcgctcggtg cgcaaggctc
    18601 gggcctgacg gtgctggggc gaggcgtgct cgaggcgctg ggcatcgagg tgcagccgat
    18661 caccctggaa aaagcggccg acgggccggc gatggtgctg gacgggcgcg cggccgcgct
    18721 gtggggcgcg ggcgtcggct ggcccgggtt caccgccctg gcgaaggcgg gcgggaggtt
    18781 cgtcgcgccg agcgccgccg agatccagca gatcctggcg aagaacagcg ccctcaaggc
    18841 gctcaccatg cccgcgggca gctatcccgg gcaggacgcg ccggtgcata ccgtgggctc
    18901 gtggagcttc gtgctggcca ggcccgagct tcccgacgac gtcgcctacc gcctggcgcg
    18961 cgccgtccac cgcgccgagg cgccgttcgc cgcgcgcctc gcgcaggcgc gcgagacgac
    19021 gatggccaac accgccgccg ccgccccggc gccggagctc atccacccgg gcgtcaggaa
    19081 atacctcagg gaaatcggcg cggaaaaata gggtctgacc ctatttttca accggcgaag
    19141 cgcccctcgg gcagtccctg caccccgggc cgcagcgcga gcaggctccc ggcgagcggc
    19201 tgcctggcca gttcctccgg cgacaggcgc tgccttgccg tggtgacgta gagcgtgccg
    19261 aggtcctcgc cgccgaagca gcagcaggtc ggattggcca ccggcacttc gaccacgcga
    19321 tccacctttc ccgccggcgt gtagcgcacc aggcgccagc cgccgtattc cgcgttccac
    19381 aggcagccct cggcatccac gcaggagccg tcgggccgcc ccgctcccgc gtcggcgaac
    19441 acgcgctcgt tcgtcgccgc gccgctggcg gggtcgtagt cgtaggccca gaggatgtgg
    19501 cgcatggtgt cggcgaaata catcctgccg ccgtcggggc tgaaggcgag gctgttggga
    19561 atgacgacgg cgttgcgcag cctggtgcag gccatgtcgg cgtcgaagcg gtacagcgac
    19621 cccaccggat cgcgcttcac gtcgctcatg gtgccggccc agaagcgccc ggcgcggtcg
    19681 cagcgcccgt cgttgaagcg gttctcgggc agcgcggcct cgggattggc gcgcgcctcg
    19741 agcgcgccgc tcgccgcgtc gaggaaatgg atgcccgatt tggtcgccgc gaggaggccg
    19801 ccctgctcgc ggaacgcaat gctgcccacc acctcgggca gcaccagcat catcaccgcg
    19861 ccgctcgccg ggtcgagctg cttcaccgcc ggccagcggc tgtcgaccca ccacaggagc
    19921 ctgccgcgct cgtcccacag cgggcactcg ccgagcaggt ccgcgccgcg cgccgcgacc
    19981 tccacctgga acgccattgc ccgttcgtct ccccgcaggc acgatagcac gcatgaaagt
    20041 cgtgttctac ggctcgctga tccaccatct caccgacacg ctgctcgcca aggtcaagac
    20101 gccctgggag acggcgcgct tcctggagaa ggatccgccg gaggcgctcg cgcgcgcgct
    20161 ggcgcaggcc gacgccctga tctcgctggt gtggaaccgc tcgttccccg cggcgccgcg
    20221 cctgaagctc atccagtcgc ccggcaccgg cgtggacgcc atcgatttcg ccgccgtgcc
    20281 gccgggcgtg cgcgtgtgca actgctacgg ccacgccgag ggcaccgcgg agtacgtgct
    20341 gctgtcgatc ctgctctggt gccacgtcaa tttcgtgccc gcgcaccaga gcttccgcgc
    20401 cgagcgcagc tggcgctaca gcgggcgcgt caacggcccg atcaacgagg agctccacgg
    20461 caagacggtg ggcatcctgg gcctgggcgc catcggcagc gcggtggccg cgcgcgccaa
    20521 ggccttcggc acgcgcgtgc tgggctgcaa ccgcacgccg cggaacgtcg agtgcgtcga
    20581 caagcaatac ccgctcgcgc agatcgacga attcctcgcc gagtgcgatt tcgtggtgat
    20641 cgccgccgcg ctggcgccgg agaccgagcg cctgatcgac gccgggcgct tcgccaggat
    20701 gaagccctcc gcggtgctgg tgaacgtcgg ccgcggcgcc atcgtcgacg aggacgcgct
    20761 gtacgccgcg ctgaaggacc ggcgcatccg cggcgcgacg ctcgacgtct ggtacgccta
    20821 cccgcgccac gacgacctgg cggtggcgcc ctcgaggcac gatttcttcg cgctggacaa
    20881 cgtctggatg acgccgcaca cctcggcctg gaccaccggg atggtcgagc ggcgctggag
    20941 cgaaatcgcg gcgaacctgg atgcgctcgc gcgcggcgcg ccgctgcgcc accagctgcg
    21001 atgaagctcg agcgctggtc gctgcagtgc taccgcctgc cgtacctgcg cgaggtggtg
    21061 tgggccaacg cgcgcgagga tgcgggggtg ttcgcgctgc tcaagctgga ggcggcgggc
    21121 tgcaccggca tcgcagaagg cacgctgaag gacacttgga gcggcgcgtc gccgaagtcg
    21181 ctcgccgcgg cgctggagga tttcctcatc ccgcggctgc gcgccgcgga cctcgccgac
    21241 gagctcgcgg tggcgcgcgc cttcgccggc atcccggaga accggctcgc caagggcatg
    21301 atcgagagcg cctgctggac gctgcgcgcc gctgtcgccg gcgcgccgct gtggaagctg
    21361 tggggcgcgg ggcgctcgcg cgaagtggcc tggaccgtca cgcgccaggc gcccaaggcg
    21421 atggcggtcg aggcggtgga catgtgcgcg caatacggct ttcgcacgct gaaggtgaag
    21481 ggcgggcagg gcgtggaggt ggacctcagg gcgctggcgg agatccagtc cgcggtgggc
    21541 ggcggcgtgg agctctacgt ggacgccaac agcgcctacg cgcgcgagga tgcactcgaa
    21601 tacgtgcagg ggctcgccag cgccggcgtc acggtggccg aggacccctg cgcgctgcag
    21661 ccggaccggc atttcgagga gctgcagagg gcgagcccga tcccgatcct ggtggatcgc
    21721 agctgcgcct cgaaggagga cgcggcgctg tacctggagc gcggggcgaa ggccctgtcg
    21781 acgaagccgg ggcgggtggg gttggcggaa acgcgcgcca tcgcggcgct ggcggcggcg
    21841 cgcggcgcga agaccgcggt ggggatctac gccgagagcg cgctggggac gctcgtcaac
    21901 ctgcagcagc cggggaccat ggccgcggag cagacgttct tcctcgccat gaaggagcag
    21961 gtgagccgcg tgctgcccga aatccgcggc gggcggatcg aactgccggc ggaggcggac
    22021 ctggcgcagc tggtggattg ggggcgcctc gcgcaattgg cctacaatag ctagttgtcc
    22081 ggcccagcgc cgctactaga tatatggcca aacaacagat ctacgacgcc tccgccatcc
    22141 aggccctcaa gggcctggag ccggtgcggc gcatgccggg gatgtacacg cacaccgtgc
    22201 atcccctgca catcgtgcag gaggcgatcg acaacgcggt cgacgaggcg ctcgccggct
    22261 tcggcaagcg cctcggcgtg cgcgtgttca aggacggctc ggtcgaggtc gaggacgagg
    22321 gccgcggcat cccggtggac atgcacccgg aggagaagaa gcccgcggtg gaggtggcct
    22381 tcggcatgct gcacgccggc ggcaagttcg cgaagtccgg caccggcgtg taccacatct
    22441 cgggcggcct gcacggcgtg ggagtcgcgg tgtcgaacgc gctgtcccgg cggctggaag
    22501 tcaccgtgta ccgcgacggc ggcaggcact cgatcgcctt cgagggcggc gagctcaaga
    22561 acaagctgaa gagcgagcgc atcaaggaaa agcgcagcgg caccgtggtg cgcctgtggc
    22621 cggatccgaa gtacttcgat tcgccgaaca tcccgctgcc cgagctcgag cacctggtgc
    22681 gctcgaaggc ctacctgctg cccgggctca ccaccgtgct cgaggtggaa ggcaaggaga
    22741 agaaatcctg gaagtacgag cgcggcatgg cgcagtactt cgagttcatg ctcgaggggc
    22801 gcgagctggc ggcgccgctg ttcaccggcg agcgctactt cgccgaatcg agcatcccgg
    22861 agatcgagcc cggcgagggc gcgtcctggg ccatcggctg ggtggccgag ggcgaggtgt
    22921 tcgccgacag ccacgtgaac ctcatcccga cgcgctcggg cggcacgcac gaggcggggt
    22981 tccgcgccgg ggtgttcgac gccctcggcg ccttcatgga ggcgcgcgcg ctcgcgccca
    23041 agggcgtgaa gctgatcgcc gacgacgtct gggcgcgcgc ctgcttcacg ctctcggcgc
    23101 ggatcgtgcg cacgcagttc cacggccaga ccaaggagaa gctcaccacg cgccacgcgg
    23161 cgaagctgct ggagctgtgc gtgaaggatg ccttcgagct gtggctcaac gagcacccgg
    23221 aagagggcaa gcgcatcgcc gagctcgcca tcgagcaggc gctcgcgcgc ctcgccaagg
    23281 gcaagaaggt cgagcgcaag aaatcctcgg gcatcgccac gctgccgggc aagctcgtcg
    23341 actgcgcctc gggcgacgtg agccggaatg aattgttcct ggtggagggc gattcggcgg
    23401 gcggctcggc gaaggaggcg cgcgacaagg agtcgcaggc gatcctgccg ctgcgcggca
    23461 aggtgctcaa caccatgtcg aaggacagcc gcaccgtgct cgccaacaag gaattgcagg
    23521 cgatcgccac cgccatcggc gtggacccgc acgggcccga ggacgcggcc gacctcggcg
    23581 gcctgcgcta cggcaaggtg atcctgatga ccgacgccga cgtggacggc gggcacatcc
    23641 aggcgctgct gctcaccttc ttcttcatgc acgcgccgaa actggtggag gccgggcacg
    23701 tgtacgtggc gcagccgccg ctcttcaagg tggagatccc ggcgcagggc aagggccggc
    23761 cgcagcggcg cgcctattgc ctcgacgacg acgagctgga cgagacgctc gccgcgctga
    23821 agaaggagaa agtgaaggaa ggctcctggg agatctcgcg cttcaagggc ctgggcgaga
    23881 tgagccccga gcagctgtgg gaaaccacca tgaacccgga cacgcggcgc ctggtgcgca
    23941 tgcagctcga cgcgaagcag ttgaagaagg cgcgcgagac cttcgagctg ctgatggact
    24001 ccggcgaggc cgaaggccgg cgcaactgga tgcgcgagca ctggaagacg gtggaggcgg
    24061 atatctaggg agggcgccat ggcgaagaag cagaaggcgt tctgggagcg ggcctacaag
    24121 ggccacgtgc tctggcaggg caggcagaag ctgggcaagg tgacgctggc gggcgaaggc
    24181 cgctacacct gggaggccgg cagcagggca ggggcgagcg acgacctgga gaaggcgaag
    24241 aaggccgtcg agctcgcggt ggcgatggct gacaagcaac tcgacctctt caggtgacca
    24301 tggacacgcg cacccccgat ctcttcaccc ccgaaaacgg cgacgacgcc gcgccgatcg
    24361 agcgccacgc ctcgcaggcc tacctcggct acgcggtctc caccgtcaag tcgcgggcgc
    24421 tgcccgagat cgccgacggc ctgaagcccg tgcagcgcag gatcctgtac gccatgggcg
    24481 acgccgccac gcaggggttt gcgaaatgcg cgcgctacgt cggcgaggtg ctcggcaagt
    24541 accacccgca cggcgactcc tcgacctacg aggcgctcgt gcacctcgcg cagcccttct
    24601 ccatgcgcta cccgctgatc gacggccagg gcaacttcgg ctcgcgcgac ggcgactcgg
    24661 cggcggccta ccgctacacc gaggcgaagc tgtcgcgcta cgccgagctg atgctcgccg
    24721 agatcggcga gggcacggtg gatttcatca agaactacga cggcaaattc gaggagccgg
    24781 tgctgctgcc ggcgcgcctg cccttcggcc tgctgaacgg ctccttcggc attccggtcg
    24841 gcttctccac ccgcatcccg tcgcacaacc tgcgcgaagt ggccgaggcg gccgcgcacg
    24901 tcatcaagca cccgaaagcc agcgtcaagg acgtgctggc gatcctgccg gggcccgatt
    24961 tcccgggcgg cggccaggtg atctcgccgc cggaagaaat ccggcaagcg tacgaggccg
    25021 gccgcggctc gctcgtcctt cgctgcaagt gggagcggga aaacctcgcg cgggg
//
LOCUS       DASRIE010000010        15491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2092631, whole genome shotgun sequence.
ACCESSION   DASRIE010000010 DASRIE010000000
VERSION     DASRIE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092631"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="amrS"
                     /locus_tag="VFB53_00385"
     CDS             complement(<1..876)
                     /gene="amrS"
                     /locus_tag="VFB53_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZN85216.1"
                     /translation="MAAAGAALEGAYPGRWWHALEDGRVQCDLCPRDCRLHEGQRGMC
                     FVRKMEGGRMVLTTYGRSSGFCVDPIEKKPLNQFYPGTSVLSFGTAGCNLACKFCQNW
                     DISKSRDLDRLQDQASPEAIAQAAVELGCRSVAYTYNDPVIFAEYAMDIADACRAKGV
                     QNVAVTAGYIHPQARREFYAKMDAANVDLKGFTEEFYFKICGAHLAPVLETLVYLVKE
                     TQVWTEITTLLIPGKNDSDGELEAESKWIFENLGPDVPLHFTAFHPDWKMTDIAATPA
                     ATLTRAREIALRAGLR"
     gene            complement(852..2216)
                     /gene="amrB"
                     /locus_tag="VFB53_00390"
     CDS             complement(852..2216)
                     /gene="amrB"
                     /locus_tag="VFB53_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZN85217.1"
                     /translation="MSEVRPAAVAGAFYPRDARELESEIAELLDGVENLAPRFGHPKA
                     LIVPHAGYIYSGPVAARAYDELAAARGVVKRVVLLGPVHRVPVRGLALPGAEAFDTPL
                     GRVPVDAEAVRLLAPHRQVVASPEAHAMEHSLEVQLPFLQKMLGEFALLPLAVGDARP
                     QEVREVVERLWGGPETLFVLSTDLSHYHAYAAARAIDQATLARIASFDTDINHEEACG
                     ATPLNGFLAAAKARGLSIRLLGACNSGDTAGGKDRVVGYAAFALYEGARVSLEDAGLA
                     LLAIARAAIGHALGVHAEPPHTDFAPWLLRPGASFVTLMLEGKLRGCIGSLAAARRLG
                     EDVAANALGAALRDPRFSPLTAAEWPLCQVEVSVLSPAKPLRFADEAEMLAQLRPGED
                     GVILEYEGRRATFLPQVWDALPDRRQFLEELMRKAGIPADTRLARCKLWRYRVVKWQQ
                     PALH"
     gene            complement(2213..2680)
                     /locus_tag="VFB53_00395"
     CDS             complement(2213..2680)
                     /locus_tag="VFB53_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZN85218.1"
                     /translation="MDDLARKLGAALKARGLMLVTAESCTGGWAATALTAIAGSSEWF
                     ERGYVTYSNAAKREELGVSEGTLQRHGAVSEETAREMAAGALKHGRGQVALAITGVAG
                     PTGGSPGKPVGTVCFAWAQGSKISSETRRFDGDRESVRRQSVVRALQGALELL"
     gene            complement(2668..3165)
                     /locus_tag="VFB53_00400"
     CDS             complement(2668..3165)
                     /locus_tag="VFB53_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZN85219.1"
                     /translation="MIVFRPTARFVFTHPAHFLAFGFGAGLSPFAPGTAGTLLAFPIW
                     WAIGGLFEPAVLVGVLAILFLAGVWACGVAGRHLGIADHGGMCWDEVVAFLLILAIVP
                     PDPWWQAAAFFLFRAFDVVKPPPIRQLESRMKGGFGVMFDDLLAAGYTLLLLALARRL
                     LPWMI"
     gene            complement(3162..4079)
                     /gene="thiL"
                     /locus_tag="VFB53_00405"
     CDS             complement(3162..4079)
                     /gene="thiL"
                     /locus_tag="VFB53_00405"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZN85220.1"
                     /translation="MPSEFDIIRRHFSRPARQALLGTGDDAALLRPDAGAALAVSTDL
                     LLEGRHFPTGAAPRALGHKALAVNLSDMAAMGATPCWATLALALPAADDAWIGEFAEG
                     FYALAARYRVDLVGGDTTRGPLAICVTILGEVPEGLALMRGGARPGDDIWVSGALGGA
                     ALALHGAGGAAAARRLHEPEPRVELGERLRGIASAAIDVSDGFAQDLGHILERSGAGA
                     LVHYALLPKHPCDDAGLQQRCVLAGGDDYELAFTAPQGARAELEALAGTLGLALTRVG
                     SMQRGEPRLQLLDAQGAAMAVPRGFDHFA"
     gene            complement(4089..4526)
                     /gene="nusB"
                     /locus_tag="VFB53_00410"
     CDS             complement(4089..4526)
                     /gene="nusB"
                     /locus_tag="VFB53_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZN85221.1"
                     /translation="MKGAKTARRRSREAALQALYAWQLAGGDALEKLNALDEDARSDA
                     ALAQSIVRGVFERSGELELMIAPCLDREFRRLSPVERAILYIGAYELSAHPETPFKVV
                     VNEAIELGKSFGGTGGHRFVNGVLEKLAGILRPQEVARGRQPA"
     gene            complement(4523..4942)
                     /gene="ribH"
                     /locus_tag="VFB53_00415"
     CDS             complement(4523..4942)
                     /gene="ribH"
                     /locus_tag="VFB53_00415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZN85222.1"
                     /translation="MRLGIVASRFNAELSAKLLQRAQAEAARLGARQVVVEQVPGALE
                     IPLALQWMAQSGRFDCLAAIGCVVRGDTYHFEIVASESARGVMDVALEFGIPVANGIL
                     TTDTEEQAKARLDKGAEAVRVALEMAALRAKVAAVPR"
     gene            complement(4945..6057)
                     /gene="ribBA"
                     /locus_tag="VFB53_00420"
     CDS             complement(4945..6057)
                     /gene="ribBA"
                     /locus_tag="VFB53_00420"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZN85223.1"
                     /translation="MRRETAAISPVADIVADLRAGRMVVLVDDEDRENEGDLVFAAEF
                     VTAEKINFLATHGRGLICMPITEDHALRLGLRPMVEQNRSRHGTNFTVSIEAAEGIAT
                     GISAADRALTVKVAAAPGARAEDIVQPGHVFPLIAQDGGVLVRAGHTEACCDLARLAG
                     LTPAAVLCEIMKADGTMARLPDLLEFSAQHGLKIGTIADLIEYRSRNESLVKRSFERD
                     IDTAWGRFRLVSFRDLPTRATHLALVAGRPRADAETLVRVHEPLSVIDLLDAGPGTHS
                     WGVGAALAAIAEAGTGVMVLLNCSESSAALDARLAEARRERQGRSMDLRNYGIGAQIL
                     RDLGVGRMRLMAAPRKMPSMAGFGLEVTGHVESPGR"
     gene            complement(6125..6496)
                     /gene="rplS"
                     /locus_tag="VFB53_00425"
     CDS             complement(6125..6496)
                     /gene="rplS"
                     /locus_tag="VFB53_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZN85224.1"
                     /translation="MNLIQKLEQEEIKRLGKTVPDFSPGDTVIVNVNVVEGERKRAQA
                     FEGVVIAKRNRGIGSSFTVRKISSGEGVERTFQTYSPLIASVEVKRKGEVRRAKLYYL
                     RQRSGKSARIRERIAGEGVEE"
     gene            complement(6493..7239)
                     /gene="trmD"
                     /locus_tag="VFB53_00430"
     CDS             complement(6493..7239)
                     /gene="trmD"
                     /locus_tag="VFB53_00430"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZN85225.1"
                     /translation="MRFDVVTLFPEMFAAVTASGIPRKALEAGLWRLQAWNPRDFTTD
                     SYRTVDDRPYGGGPGMVMLAEPLEKALEAAKAAGGGKVIYLTPQGRKLDHAKVMELSG
                     RRAVTLLCGRYEGVDERLVRRRVDEELSLGDFVLSGGELAAMAVIDAVVRQLPGALGD
                     GQSAVEESFAAGLLDCPQYTRPEVYAGEGIPQVLLSGHHEQIRRWRLQQALGRTWLRR
                     PELIAARRLSDEERKLLEEFKRDMESGAEK"
     gene            complement(7239..7739)
                     /gene="rimM"
                     /locus_tag="VFB53_00435"
     CDS             complement(7239..7739)
                     /gene="rimM"
                     /locus_tag="VFB53_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZN85226.1"
                     /translation="MARRPPELIELGRVAGSYGVRGWVKVAPQRGVAEALVAARQWWL
                     GDKAWRVEEARVHSATVIARLAGIGTREQALALKGSRISVPRSALPDAQDGRYYLADL
                     VGLDVVNAEGQKLGVVRQWFSNGPQDVMQLAGGDRERLLPWVPSVVKKVDLEARRVEV
                     EWQADW"
     gene            complement(7720..7971)
                     /gene="rpsP"
                     /locus_tag="VFB53_00440"
     CDS             complement(7720..7971)
                     /gene="rpsP"
                     /locus_tag="VFB53_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZN85227.1"
                     /translation="MVVIRLARGGANKRPFFSLVVADSRRAVKGKFIERVGFYDPKAP
                     EGRESLRVNVERLKHWQGKGAQLSPTVKRLVKQFGKKAA"
     gene            complement(8020..8778)
                     /locus_tag="VFB53_00445"
     CDS             complement(8020..8778)
                     /locus_tag="VFB53_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZN85228.1"
                     /translation="MSRGFGALVIGDEILVGKRQDKHFPWLIEALARRGLRLAWCEHL
                     GDEPARIVAALRRTLATEDIVFSFGGIGATPDDHTRQCAAEALGVALALHPEAEREIR
                     GRFQAEATPQRLKMGEFPDGAQIIPNPFNRIPGFSVRDHHFVPGFPQMAWPMVEWLLD
                     TRYRHLLDSQRWAEASVIVYDLPESTIAPLMEELNAQHPGLKAFSLPSMGEGGTRRHI
                     ELGVRGVPAEVGPAMERLRRGIDALGGRMEIPNR"
     gene            complement(8775..9491)
                     /locus_tag="VFB53_00450"
     CDS             complement(8775..9491)
                     /locus_tag="VFB53_00450"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HZN85229.1"
                     /translation="MNAVVRSLVFALGNLLHPKMLWLMLWPLLIALALWGTLAIVYWA
                     QLAMWLAGLINQWLTTGWFALQWDLQGAASIAAKILILIMLVPLVQLTALLILSSFGM
                     PAMVDHVAARAYPALERRKGGSLAGSLWNGAVALCGMLLLFIASIPFWLFPPLWPMIP
                     VAVLGWVNQRVLRYDALAEHADAAEMRRVFAGRRGNMYLLGLVLALMAYIPLIGFFAP
                     VLFGLSFIHYLLGELRALRP"
     gene            complement(9488..13111)
                     /gene="hrpA"
                     /locus_tag="VFB53_00455"
     CDS             complement(9488..13111)
                     /gene="hrpA"
                     /locus_tag="VFB53_00455"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZN85230.1"
                     /translation="MQPQIHFDPALPVTQKREEIARAVAAHPVVIVCGETGSGKTTQI
                     PKILLEMGRGEKKHIGHTQPRRIAARSVAARIAEELKVELGGLVGYKVRFTDKVGPRT
                     QVKLMTDGILLAETQGDPELRAYDTVILDEAHERSLNIDFLMGYFKRLLPRRPDLKLV
                     ITSATIDAERFSKHFDGAPVIEVSGRLYPVEVRYRPVGGDAEDTTRDEEEQALVDAVE
                     ELFRVGEGDVLVFLPGEREIRDAAAVLGGAKLKGAEILPLYGRLSVAEQDRVFHPGGA
                     RRVVLATNVAETSLTVPRIRYVVDAGQARVKRYSYRNKVEQLRVENISQAAAQQRAGR
                     CGRVADGICIRLYGEDDFKARPAYTDPEVLRSSLAAVILRALALNLGKVEDFPFLDAP
                     NSRAVADGYGLLAELGAVDEDNALTGIGRELAGLPLDPRVGRMLIAARAEGCLEQLLV
                     IAAALSVQDPRERPLARQGAADERHAKFADERSDFLALLKLWRLQGEQGLRRLCRDNF
                     LSYPRMREWRDVHQQLQTAVGELEWKMSSARMDKPDGYRAIHRALLAGLLGNIGMKDE
                     ADDNYTGARGIKFRVHPGSGTKKPGKWVMAAELVETTRLYARTVATIDAQWLEDLGAH
                     LLKRHRDNPHWEKMRAQVVAVERGTPYGLPVYTGRRVHYGPHDAVLSRDIFLRSALVE
                     GALDSKAPFFAHNRRLVAEIERMEHKSRRPDILVDDELILAFYDERVPAGIHNGADFE
                     KWRKEAERGDPKLLFLRRDDLMRHEAAGITTGNFPHELKLGPNEFRLEYHFEPRSPRD
                     GVTMTVPVALLNQVPAARGEWLVPGLLKEKVRALAKSMPQRLRHKLGPLEEFAEAFAA
                     KAPPSDTPLAQALARHMRSELQLEVPLDAFRPDSAPPHLHMNYLVIDEHGRQLAMGRN
                     LAELKRELGEHTAAVLQEEAPLAEGERYTGWTMGDLAEVMEIARGGQTLVGYPALVDA
                     GEAVTLQVFDAPDKARELHRAGVRRLLAIAFKDRVRELERGWARDVALGPLKEDLVAA
                     VLDRVFLGEAAPMAQADFARRVAEGRARLLLVAQEMARTVSAILAAHADLQKKLAQAA
                     KTHPQAAEDVKQQMARLLGGRWLAATPWARLQHTPRYLKAAALRVEKLRADPARDARL
                     AAQLQPLLAAWLRAPRPLTAELEQFGWLLEELRVSLFAQELKTPVPVSAKRLAKLWQS
                     IRR"
     gene            13146..13754
                     /locus_tag="VFB53_00460"
     CDS             13146..13754
                     /locus_tag="VFB53_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN85231.1"
                     /translation="MTGPTKEFWEQRFAAGNTPWDRGASSPQLAAWLSAGALRPCRIL
                     VPGCGSGYEVADFAMAGFEVTALDYADEAIARTHRRLREAGLDATLVQADALAWSPDR
                     PFDAVYEQTCLCALYPDQWRDYADQLHRWLAPGGRLYALFVQFARPGAAAGAIEGPPY
                     HCDINAMRALFPAPRWAWPKPPYPRTTHPRGLEELAVVLERR"
     gene            complement(13751..14266)
                     /locus_tag="VFB53_00465"
     CDS             complement(13751..14266)
                     /locus_tag="VFB53_00465"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HZN85232.1"
                     /translation="MSTSVRAIRADITTLDVEAIVNAANPTLLGGGGVDGAIHRAAGP
                     DLLRECRALGGCATGDAKLTKGYRLRARYVIHTVGPVWHGGAKGEPELLASCYRRSLE
                     LAVANGIASIAFPGISTGVYGYPVEAAAKIAVRTVRAALQGTKGMTEVVFCCFSEADL
                     KVYESILAASD"
     gene            complement(14266..14823)
                     /locus_tag="VFB53_00470"
     CDS             complement(14266..14823)
                     /locus_tag="VFB53_00470"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZN85233.1"
                     /translation="MFSGIVQAVGKIVSAKPFVVDAGKLPLGDVKVGDSICVQGACLT
                     VTKKRGKRLSFDVSQETLDCTTGLARPGPVNLEKALRLADRLGGHLVSGHVDGVGLVT
                     QVKGSVYTFRAPKAVAKYVAPKGSICIDGVSLTVNRVKGAEFEVNLIPFTLKVTTLAR
                     LKPGSEVNLEADLLARYVEGFSKSA"
     gene            complement(14823..>15491)
                     /gene="ribD"
                     /locus_tag="VFB53_00475"
     CDS             complement(14823..>15491)
                     /gene="ribD"
                     /locus_tag="VFB53_00475"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZN85234.1"
                     /translation="ARELNIGFVSRMTRGRPWVRMKIAATLDGRTALANGKSQWITGA
                     EARKDGHRWRARACAIATGGGTVKTDDPQLTVREVETPRQPVRVVIDSHVETPARAKV
                     LQGEKALVFAAVKGKSLPNAEIVVLPNADGKVDLQKMLQELGRRGINELHVEGGFKLN
                     GSLVREGCVDEFLIYLNPSFLGDSAQGMVNLQDMASLDQRVALKIRSVERVGEDLRIL
                     ARPA"
BASE COUNT         2206 a         5779 c         5253 g         2253 t
ORIGIN      
        1 gcgcaggccc gcgcgcaggg ctatttcacg cgcccgcgtc agcgtcgccg ccggcgtggc
       61 cgcgatgtcg gtcatcttcc agtccggatg gaaggccgtg aagtgcagcg gcacgtccgg
      121 cccgaggttc tcgaagatcc acttcgactc ggcctcgagc tcgccgtcgg aatcgttctt
      181 acccgggatc agcagcgtgg tgatctcggt ccacacctgc gtctccttca ccaggtagac
      241 gagcgtctcg agcaccggcg cgaggtgcgc gccgcagatc ttgaaataga actcctcggt
      301 aaatcccttc aggtccacgt tcgccgcgtc catcttggcg tagaactcgc gccgcgcctg
      361 cggatggatg tagcccgcgg tcaccgccac gttctgcacc cctttcgcgc ggcaggcgtc
      421 ggcgatgtcc atcgcgtact cggcgaagat caccgggtcg ttgtaggtgt aggccaccga
      481 gcggcagccg agctccaccg cggcttgcgc gatggcttcc ggagatgcct ggtcctgcag
      541 ccggtccagg tcgcgcgact tggagatgtc ccagttctgg cagaacttgc aggcgaggtt
      601 gcagcccgcg gtgccgaagg acagcaccga ggtgccgggg tagaactggt tgagcggctt
      661 cttctcgatc gggtcgacgc agaatcccga ggagcgcccg taggtggtga gcaccatgcg
      721 gccaccctcc atcttgcgca cgaagcacat gccgcgctgg ccctcgtgca ggcggcaatc
      781 gcgcgggcac aggtcgcact gcacgcgccc gtcctcgagc gcgtgccacc agcgcccggg
      841 gtaggcgccc tctagtgcag cgccggctgc tgccacttga ccacgcggta gcgccacagc
      901 ttgcagcgcg cgaggcgcgt atcggcgggg atgccggcct tgcgcatcag ctcctcgagg
      961 aactggcgcc tgtccggcag cgcgtcccag acctgcggca ggaacgtggc gcgccggcct
     1021 tcgtattcga ggatcacccc gtcctcgccg gggcgcagct gcgcgagcat ctccgcctcg
     1081 tcggcgaagc gcagcggctt tgccggcgag agcaccgaca cctcgacctg gcagagcggc
     1141 cactccgccg ccgtgagcgg ggaaaagcgc gggtcgcgca gcgcggcgcc gagcgcgttc
     1201 gcggcgacgt cctcgcccag gcgccgcgcc gcggcgaggc tcccgatgca gccgcgcagc
     1261 ttcccctcca gcatcagggt gacgaagctc gccccgggcc gcagcagcca gggcgcgaaa
     1321 tcggtgtgcg gcggctcggc gtgcacgccc agggcgtggc cgatggcggc gcgcgcgatg
     1381 gcgagcagcg cgaggcccgc gtcctccagc gatacgcgcg cgccttcgta cagcgcgaac
     1441 gcggcatagc ccaccacccg gtccttgccg cccgcggtgt cgcccgaatt gcacgcgccg
     1501 aggaggcgga tcgacagccc gcgcgccttg gcggcggcga gaaagccgtt caacggcgtc
     1561 gcgccgcagg cctcctcgtg gttgatgtcg gtgtcgaagc tggcgatgcg cgcgagggtc
     1621 gcctggtcga tggcgcgcgc cgccgcgtag gcgtggtagt gcgacaggtc cgtggacaga
     1681 acgaacagcg tctccggccc gccccagagc cgctcgacga cttcgcgcac ctcctgcggc
     1741 cgggcatcgc ccaccgccag cggcaggagc gcgaactcgc ccagcatctt ctgcaggaac
     1801 ggcagctgca cttccagcga atgctccatc gcgtgcgcct ccgggctggc caccacctgc
     1861 cggtgcgggg cgagcaggcg cacggcttcg gcgtcgaccg gcacgcgccc gagcggcgtg
     1921 tcgaaggcct ccgcgccggg cagggcgagg ccgcgcaccg ggacgcggtg cacgggcccg
     1981 agcagcacca cgcgcttgac cacgccgcgc gcggcggcca gctcgtcgta ggcgcgcgcc
     2041 gccaccgggc cggagtagat gtagccggcg tgcggcacga tcagggcctt gggatggccg
     2101 aaacgcggcg cgaggttctc cacgccgtcc agcagctcgg cgatctcgct ttccagctcg
     2161 cgcgcgtcgc gggggtagaa cgcgcccgcg acggcggcag gacgaacctc gctcacagca
     2221 gctccagtgc cccttgcagg gcgcggacca ccgactggcg gcgcacgctc tcgcggtcgc
     2281 cgtcgaaacg ccgcgtctcg ctcgaaatct tactcccctg cgcccaggcg aagcacacgg
     2341 tgcccaccgg cttgccgggc gatccgccgg tgggaccggc gaccccggtg atggcgagcg
     2401 ccacctgccc gcggccgtgc ttgagcgccc cggccgccat ctcgcgcgcg gtctcctcgc
     2461 tcaccgcgcc gtggcgctgc aaggtcccct ccgacacccc gagttcctcg cgcttggcgg
     2521 cgttcgagta ggtgacgtag ccgcgctcga accattccga gctgccggcg atcgcggtca
     2581 gcgccgtcgc cgcccagccg ccggtgcagg actcggcggt gaccagcatc aggccgcgcg
     2641 ccttcagcgc ggcgccgagc ttgcgcgcta gatcatccac ggcagcagcc tcctggccag
     2701 ggcgaggagg agcagcgtat acccggcggc gaggagatcg tcgaacatca cgccgaaccc
     2761 gcccttcatg cggctctcca gctggcggat gggcggcggc ttgaccacgt cgaaggcgcg
     2821 gaacaggaag aacgccgccg cctgccacca ggggtccggc ggcacgatcg ccaggatgag
     2881 gaggaaggcc accacctcgt cccagcacat gccgccgtgg tcggcgatgc ccaggtggcg
     2941 cccggcgacg ccgcaggccc acacgccggc gaggaacagg atcgcgagga cgccgaccag
     3001 caccgccggc tcgaagaggc cgccgatcgc ccaccagatc gggaaggcga ggagcgtccc
     3061 ggcggtgccg ggcgcaaagg gcgacaggcc ggcgccgaag ccgaaggcga ggaagtgcgc
     3121 cggatgcgtg aagacgaagc gcgccgtggg gcggaagacg atcatgcgaa gtggtcgaag
     3181 ccgcgcggca cggccatcgc cgcgccctgc gcgtccagca gctgcagcct gggctcgccg
     3241 cgctgcatcg agccgacgcg cgtgagggcg aggccgagcg tgcccgccag cgcctcgagc
     3301 tcggcgcgcg cgccttgcgg cgcggtgaac gcgagctcgt agtcgtcgcc ccccgccagc
     3361 acgcagcgct gctgcaggcc ggcgtcgtcg cagggatgtt tcggcagcag cgcgtagtgc
     3421 accagcgcgc cggcgcccga gcgctcgaga atgtggccga ggtcctgggc gaagccgtcg
     3481 gagacgtcga tcgccgcgct cgcgatgccg cgcagccgct cgccgagttc cacgcgcggc
     3541 tcgggttcgt gcaggcgccg cgccgccgcg gcgccgcccg cgccgtgcag cgcgagcgcc
     3601 gcaccgccca gggctcccga cacccagatg tcatccccgg gccgcgcgcc gccgcgcatc
     3661 agcgccaggc cctcgggcac ttcgccgagg atcgtgacgc agatggcgag cggaccgcgc
     3721 gtggtgtcgc cgcccaccag gtccacccgg tagcgcgcgg cgagcgcgta gaagccctcg
     3781 gcgaactcgc cgatccaggc gtcgtcggcc gccggcagcg ccagggcgag cgtcgcccag
     3841 cagggcgtcg cgcccatcgc cgccatgtcg gacaggttca ccgccagcgc cttgtgcccg
     3901 agcgctcgcg gcgcggcgcc cgtggggaaa tggcggccct cgagcaagag gtcggtggac
     3961 accgcgagcg ccgcccccgc gtcgggccgc agcagcgcgg cgtcgtcgcc cgtcccgagc
     4021 agcgcctggc gcgccgggcg cgagaagtgg cgccggatga tgtcgaactc cgagggcatc
     4081 gcgcccccct aggcgggctg cctgccgcgc gccacttcct gcggccgcag gatgccggcg
     4141 agcttctcca gcacgccgtt gacgaacctg tggccgccgg tgccgccgaa gctcttgccc
     4201 agctcgatcg cctcgttgac caccaccttg aacggcgtct ccggatgcgc cgagagctcg
     4261 taggcgccga tgtacaggat cgcgcgctcc accggcgaga ggcgccggaa ctcgcggtcc
     4321 aggcagggcg cgatcatcag ctcgagctcg ccgctgcgct cgaacacgcc gcgcacgatg
     4381 ctctgcgcca gcgcggcgtc gctgcgcgcg tcctcgtcca gcgcattgag cttttccagc
     4441 gcgtcgccgc cggcgagctg ccaggcgtac agcgcctgca gggcggcctc gcgcgagcgg
     4501 cggcgcgcgg tcttggcgcc cttcaccggg gcaccgccgc caccttggcg cgcagcgcgg
     4561 ccatctcgag cgcgacgcgc accgcctcgg cgcccttgtc cagccgcgcc ttggcctgct
     4621 cctcggtgtc ggtggtgagg atgccgttgg ccaccgggat gccgaattcc agcgccacgt
     4681 ccatcacccc gcgcgccgat tcgctggcca cgatctcgaa gtggtaggtg tcgccgcgca
     4741 ccacgcagcc gatcgcggcg aggcagtcga agcgcccgga ttgcgccatc cactgcagcg
     4801 cgagcgggat ctcgagcgcg ccgggcacct gctcgacgac cacctgcctg gcgccgagcc
     4861 tcgccgcctc ggcctgcgcg cgctgcagca gcttggcgct gagctccgcg ttgaaccggc
     4921 tcgccacgat gcccaggcgc acggctagcg ccccggcgat tcgacgtggc cggtgacctc
     4981 gaggccgaat cccgccatgc tcggcatctt gcgcggcgcc gccatcagcc gcatgcggcc
     5041 gacgccgagg tcgcgcagga tctgcgcgcc gatgccgtag ttgcgcaggt ccatgctgcg
     5101 cccctggcgc tcgcggcgcg cttcggcgag gcgcgcgtcc agcgccgccg aggattcgct
     5161 gcagttgagc agcaccatca cccccgtgcc ggcctcggcg atcgcggcga gcgccgcgcc
     5221 cacgccccag gagtgcgtgc ccgggccggc gtcgagcagg tcgatcaccg acagcggctc
     5281 gtgcacgcgc accagcgtct cggcgtcggc gcgcggcctg cccgccacca gcgcgaggtg
     5341 cgtggcgcgc gtcggcaggt cgcggaagga caccaggcgg aagcgccccc aggcggtgtc
     5401 gatgtcgcgc tcgaagctgc gcttgaccag cgactcgttg cggctgcggt actcgatcag
     5461 gtcggcgatg gtgccgatct tcagcccgtg ctgcgcgctg aactcgagca ggtcgggcag
     5521 ccgcgccatg gtgccgtcgg ccttcatgat ctcgcacagc accgcggcgg gcgtgaggcc
     5581 ggcgaggcgc gcgaggtcgc agcaggcctc ggtgtggccg gcgcgcacca gcacgccgcc
     5641 gtcctgcgcg atcagcggaa acacgtggcc gggctgcacg atgtcctcgg cgcgcgcgcc
     5701 cggcgcggcg gccaccttga cggtgagcgc gcggtcggcg gccgagatgc cggtggcgat
     5761 gccctcggcg gcctcgatcg acaccgtgaa gttggtgccg tggcgcgagc ggttctgctc
     5821 gaccatcggc cgcaggccca ggcgcagcgc atggtcctcg gtgatcggca tgcagatcag
     5881 gccgcggccg tgcgtggcga ggaagttgat cttctcggcg gtgacgaact cggccgcgaa
     5941 caccaggtcg ccctcgttct cgcggtcctc gtcgtccacc aggaccacca tcctgccggc
     6001 gcgcaggtcg gcgacgatgt cggcgaccgg gctgatggcg gctgtctctc tgcgcatggg
     6061 gagattatga aacaaaaacg gggccagcgg ccccgtgagg aaaaatgggg tcagacccca
     6121 ttttctattc ttccactccc tcgccggcga tcctctcgcg gatgcgcgcg gacttgcccg
     6181 agcgctggcg caggtagtac agcttggcgc ggcgcacttc gcccttccgc ttcacctcga
     6241 ccgaggcgat gagcggcgag taggtctgga aggtgcgctc cacgccctcg ccggagctga
     6301 tcttgcgcac cgtgaaggac gagccgatgc cgcggttgcg cttggcgatc accacgccct
     6361 cgaaggcctg ggcgcgcttg cgctcgccct cgacgacgtt gacgttgacg atcaccgtgt
     6421 cgccgggcga gaagtcgggc accgtcttgc ccagccgctt gatttcttcc tgctcgagtt
     6481 tctggatcag gttcattttt ccgctccgct ctccatgtcc cgcttgaatt cttccagcag
     6541 cttccgttcc tcgtcgctca ggcgccgcgc ggcgatcagc tccggccggc gcagccaggt
     6601 cctgcccagc gcctgctgca ggcgccagcg ccggatctgc tcgtggtgcc ccgacagcag
     6661 cacctgcggt atcccctcgc ccgcgtacac ctcgggccgc gtgtactgcg ggcagtccag
     6721 cagccccgcg gcgaaggatt cctccaccgc cgactgcccg tcgcccagcg ccccgggcaa
     6781 ctgccgcacc accgcgtcga tcaccgccat cgccgcgagc tccccgcccg agagcacgaa
     6841 gtccccgagc gacagttcct cgtccacccg gcgccggaca agccgctcgt ccacgccttc
     6901 gtagcggccg cagagcaggg tcaccgcccg cctgccggag agctccatca ctttcgcgtg
     6961 atcgagcttc ctgccttgcg gcgtgaggta gatcaccttc ccgccgcccg ccgccttcgc
     7021 cgcctcgagc gccttctcca gcggctcggc cagcatcacc atccccggtc cgccgccgta
     7081 cgggcggtcg tccaccgtgc ggtagctgtc agtcgtgaaa tcgcgcgggt tccacgcctg
     7141 caaccgccac aaccccgctt cgagcgcctt gcgcgggatg ccgctcgccg tcaccgcggc
     7201 gaacatctcc gggaacagcg tcaccacgtc gaagcggatc accagtccgc ctgccattcc
     7261 acctcgacgc gccgcgcctc gaggtccacc ttcttcacca ccgacggcac ccagggcagg
     7321 agccgctccc ggtcgccgcc cgccagctgc atcacgtcct gcgggccgtt gctgaaccac
     7381 tgccgcacca cgcccagctt ctgcccttcg gcgttcacca cgtccagccc gaccaggtcg
     7441 gcgaggtagt agcgcccgtc ctgcgcatcg ggcagcgcgc tgcgcggcac cgagatcctg
     7501 ctgcccttca gcgccagcgc ctgctcgcgg gtcccgatcc cggcgagccg ggcgatcacc
     7561 gtcgcgctat gcaccctcgc ctcttcgacc cgccaggcct tgtcgccgag ccaccattgc
     7621 ctcgccgcca ccagcgcctc ggccacgccc ctttgcggcg ccaccttgac ccagccgcgc
     7681 acgccgtagg aaccggcgac gcgtcccagc tcgatcagct caggcggcct tcttgccaaa
     7741 ctgcttcacg agccgcttca cggtcggcga gagctgcgcg cccttgccct gccagtgctt
     7801 gagccgctcc acgttcacgc gcagcgactc gcggccctcg ggggccttgg ggtcgtagaa
     7861 gccgacgcgc tcgatgaact tgcccttcac cgcccggcgc gagtccgcca ccaccaggct
     7921 gaaaaaaggc cgcttgttcg cgccgccgcg cgccagtcga atcaccacca ttttggaaac
     7981 cccgtgaacc gcgaaaaggc gcggattata cgggagaaat cagcggtttg gaatctccat
     8041 gcggcccccg agcgcgtcga tcccccgccg cagccgctcc atggcgggcc cgacttcggc
     8101 cgggacgccc cgcacgccca gctcgatgtg gcgccgggtg cccccctcgc ccatgctcgg
     8161 caggctgaac gccttgaggc ccgggtgctg cgcgttgagc tcctccatga gcggcgcgat
     8221 ggtggactcg ggcaggtcgt agacgatcac cgaggcctcg gcccagcgct gcgagtcgag
     8281 caggtgccgg tagcgggtat cgagcagcca ctcgaccatc ggccaggcca tctgcgggaa
     8341 gcccggcacg aaatggtggt cgcgcacgct gaaccccggg atgcggttga aaggattggg
     8401 aatgatctgt gcgccgtccg gaaactcgcc catcttcagg cgctgcggcg tggcctcggc
     8461 ctggaagcgg ccgcggatct cccgttcagc ctccggatgc agcgcgagcg ccacgccgag
     8521 cgcctcggcg gcgcactgtc gcgtatggtc gtcgggcgtg gcgccgatgc cgccgaagga
     8581 gaacacgatg tcctcggtcg ccagcgtgcg ccggagcgcc gcgacgatgc gcgccggctc
     8641 gtcgcccagg tgctcgcacc aggcgaggcg cagcccgcgc ctcgcgagcg cctcgatcag
     8701 ccaggggaaa tgcttgtcct ggcgcttgcc gaccaggatt tcgtcgccga tcaccagggc
     8761 gccgaagccg cggctcatgg gcgcagcgcc cgcagctcgc cgagcaggta gtggatgaac
     8821 gacaggccga acagcaccgg ggcgaaaaac ccgatgaggg ggatgtaggc catcagcgcc
     8881 agcaccaggc cgaggaggta catgttgccg cgccgcccgg cgaacacgcg gcgcatctcg
     8941 gccgcgtcgg catgctcggc gagcgcgtcg tagcgcagca cgcgctggtt gacccagccg
     9001 agcaccgcca ccgggatcat cggccacagc ggcgggaaca gccagaacgg gatcgatgcg
     9061 atgaacagca gcagcatgcc gcacagcgcc acggcgccgt tccacaggct gcccgccagg
     9121 ctgccgccct tgcgccgctc gagcgccggg taggcgcggg cggccacatg gtccaccatc
     9181 gccggcatgc cgaagctgct gaggatcagc agcgccgtga gctggacgag cggcaccagc
     9241 atgatgagga tgaggatctt ggcggcgatg ctcgccgcgc cctgcaggtc ccactgcagg
     9301 gcaaaccacc cggtggtgag ccactggttg atgaggcccg cgagccacat cgcgagctgc
     9361 gcccagtaca cgatggcgag cgtgccccac agcgcgagcg cgatcagcag cggccagagc
     9421 atcagccaca gcatcttcgg gtgcagcagg ttgcccagcg cgaagacgag cgagcgcacg
     9481 acggcgttca tctgcggata ctttgccaca gtttggcgag gcgcttggcc gacaccggca
     9541 ccggcgtctt cagttcctgc gcgaacagcg acacgcgcag ctcctccagc agccagccga
     9601 actgctcgag ctccgcagtg agcgggcgcg gcgcgcgcag ccacgcggcg agcagcggct
     9661 gcagttgcgc ggccagtcgc gcatcgcgcg ccgggtccgc gcgcagcttc tccacgcgca
     9721 gcgccgccgc cttgaggtag cgcggggtgt gctgcaggcg cgcccagggc gtcgcggcga
     9781 gccagcgccc gccgagcagg cgcgccatct gctgcttcac gtcctcggcc gcctgcggat
     9841 gggtcttcgc cgcctgcgcg agtttcttct gcaggtccgc gtgcgcggcg aggatggcgg
     9901 aaaccgtgcg cgccatttcc tgcgcgacca gcaggagccg cgcacggccc tcggccacgc
     9961 gacgggcgaa atccgcctgc gccatcggcg ccgcctcgcc gaggaacacg cgatcgagca
    10021 ccgccgcgac caggtcctcc ttgagcggtc cgagcgcgac gtccctcgcc cagccgcgct
    10081 ccagttcgcg cacgcgatcc ttgaacgcga tcgcgaggag ccgccgcacg ccggcgcggt
    10141 gcagttcgcg cgccttgtcc ggcgcgtcga acacctgcag ggtcaccgcc tcgcccgcgt
    10201 cgacgagcgc ggggtagccc accagcgtct gcccgccgcg cgcgatctcc atcacctccg
    10261 ccaggtcgcc catggtccag ccggtatacc gctcgccctc ggcgagcggc gcttcctcct
    10321 gcagcaccgc cgccgtgtgc tcccccagct cgcgcttcaa ctccgcgagg ttgcgcccca
    10381 tcgccagctg ccggccgtgc tcgtcgatca ccaggtagtt catgtgcagg tgcggcggcg
    10441 ccgagtccgg gcggaacgca tcgagcggca cctcgagctg cagttccgac cgcatgtggc
    10501 gcgcgagcgc ctgcgcgagc ggcgtgtccg agggcggtgc cttggccgcg aatgcctcgg
    10561 cgaactcctc cagcgggccg agcttgtggc gcaggcgctg cggcatcgac ttggcgagcg
    10621 cgcgcacctt ctccttcagc aatccgggca ccagccactc gccgcgcgcc gcgggcacct
    10681 ggttgaggag cgccacgggc acggtcatgg tcacgccgtc gcgcggggag cgcggctcga
    10741 agtggtactc gagccggaac tcgttcggac cgagcttgag ttcgtgcggg aaattcccgg
    10801 tggtgatgcc cgccgcctca tggcgcatca ggtcgtcgcg cctgaggaac agcagcttcg
    10861 gatcgccgcg ctcggcctcc ttcctccact tctcgaagtc ggcgccgttg tggatgcccg
    10921 ccggcacgcg ctcgtcgtag aaggcgagga tcagctcgtc gtcgaccagg atgtcggggc
    10981 ggcgcgactt gtgctccatg cgctcgatct ccgccaccag gcgccggttg tgggcgaaga
    11041 acggcgcctt gctgtcgagc gcgccctcca ccagcgccga gcgcaggaag atgtcgcgcg
    11101 aaagcaccgc gtcgtgcggc ccgtagtgca cgcgccggcc ggtgtacacc ggcagcccgt
    11161 agggcgtgcc gcgctccact gccaccacct gcgcgcgcat cttctcccag tgcggattgt
    11221 cgcggtggcg cttcaagagg tgcgcgccca ggtcctccag ccactgcgcg tcgatcgtcg
    11281 ccaccgtgcg cgcgtacagg cgcgtggtct ccaccagctc ggccgccatc acccacttgc
    11341 ccggcttctt cgtgcccgag cccggatgca cccggaactt gatgccgcgc gcgcccgtgt
    11401 agttgtcgtc cgcctcgtct ttcatgccga tattgccgag cagccccgcg agcagcgcgc
    11461 gatggatcgc gcgataaccg tcgggcttgt ccatcctggc gctcgacatc ttccattcca
    11521 gctcgcccac cgcggtctgc agctgctggt gcacgtcgcg ccactcgcgc atgcgcgggt
    11581 acgagaggaa attgtcgcgg cacaggcgcc gcagcccctg ctcgccctgc agccgccaca
    11641 gcttgagcag cgcgaggaaa tccgagcgct cgtcggcgaa cttcgcgtgg cgctcgtccg
    11701 cggcgccctg cctggcgagc gggcgctcgc gcgggtcctg taccgagagc gccgccgcga
    11761 tcaccagcag ctgctcgagg cagccctcgg cgcgcgccgc gatcagcatg cgcccgacgc
    11821 gcggatcgag gggaagcccc gccaactccc gcccgattcc ggtcagcgca ttgtcctcgt
    11881 ccaccgcgcc gagttcggcc agcaggccgt agccgtcggc gacggcgcgc gaattcggcg
    11941 cgtcgaggaa cgggaagtcc tccaccttgc ccaggttgag cgccagcgcg cgcaggatca
    12001 ccgcggccag cgacgagcgc agcacctccg ggtcggtgta ggcgggacgc gccttgaagt
    12061 cgtcctcgcc gtacaggcgg atgcagatgc cgtcggccac gcggccgcag cgcccggcgc
    12121 gctgctgcgc ggccgcctgc gagatgttct ccacgcgcag ctgctccacc ttgttgcggt
    12181 aggaatagcg cttgacgcgc gcctgccccg cgtccaccac gtagcggatg cgcggcaccg
    12241 tgagcgaggt ctcggccacg ttggtggcga gcaccacgcg ccgtgcgccg cccgggtgga
    12301 acacgcggtc ctgctcggcc accgagagcc gcccgtacag cggcaggatt tccgcgccct
    12361 tgagcttcgc cccgccgagc accgccgcgg cgtcgcggat ctcgcgctcg cccggcagga
    12421 acaccagcac gtcgccctcg ccaacgcgga acagttcctc caccgcgtcg actagcgcct
    12481 gctcctcctc gtcgcgcgtg gtgtcctccg cgtcgccgcc caccgggcgg tagcgcactt
    12541 cgaccgggta cagccgcccc gagacctcga tcaccggcgc gccgtcgaaa tgcttcgaga
    12601 acctctccgc gtcgatggtc gcggaggtga tgacgagctt caggtccggg cgccgcggca
    12661 gcaggcgctt gaagtagccc atcaggaaat cgatgttgag gctcctctcg tgcgcctcgt
    12721 ccaggatgac ggtgtcgtag gcgcgcagct ccgggtcccc ttgcgtctcg gcgagcagga
    12781 tcccgtcggt catcaacttc acctgcgtgc gcggtcccac cttgtcggtg aagcgcacct
    12841 tgtagccgac caggccgccg agttccacct tcagctcctc ggcgatccgc gccgccaccg
    12901 agcgcgcggc aatgcgccgc ggctgggtgt ggccgatgtg cttcttttcg ccccggccca
    12961 tctccagcag gatcttcggg atctgggtgg tcttgcccga gcccgtctcg ccgcagacga
    13021 tcaccaccgg gtgcgcggca acggcgcgcg cgatctcctc ccgcttctgg gtgaccggga
    13081 gcgccgggtc gaagtggatc tgcggctgca tttggcgccc gaggatatag gattggcgca
    13141 acgccatgac cgggccgacg aaggagttct gggagcagcg cttcgccgcg ggcaacacgc
    13201 cctgggaccg gggcgcgtcc agcccgcagc tcgccgcttg gctttccgcg ggcgcgctgc
    13261 gaccctgccg gatcctcgtg ccgggctgcg gatcgggata cgaagtcgct gacttcgcca
    13321 tggcggggtt cgaggtgacg gcgctcgact acgccgacga ggcgatcgcg cgcacgcacc
    13381 ggcggctgcg ggaggccggg ctcgacgcca cgctggtgca ggctgatgcc ctcgcctgga
    13441 gtcccgaccg gccgttcgac gcggtgtacg agcagacctg cctgtgcgcg ctgtacccgg
    13501 accagtggcg cgactacgcg gaccagctgc accgctggct cgcgccgggc gggcggctct
    13561 acgcgctctt tgtccagttc gcgcggccgg gcgcggccgc gggcgccatc gagggcccgc
    13621 cgtaccattg cgacatcaac gcgatgcgcg cgctgttccc ggcgccgcgc tgggcctggc
    13681 caaagccgcc gtatccgcgc actacgcatc cgcgcgggct ggaagaactg gcggtcgtgc
    13741 tcgagcgccg ctaatccgac gcggcgagaa tgctttcgta aaccttgagg tctgcctccg
    13801 agaaacaaca gaagacgacc tccgtcatgc ccttcgtacc ctgcagcgcg gcgcgcaccg
    13861 tccggaccgc gatcttcgcc gcggcttcga ccggatagcc gtatacgccg gtgctgatgc
    13921 ccggaaacgc gatcgacgcg attccgttgg ctacagccag ctccagcgag cgccgatagc
    13981 aggaggccag cagctcgggc tcgcccttgg ctccgccatg ccagaccggg ccgacggtgt
    14041 ggatcacgta cctggcgcgc agccggtatc ccttcgtcag cttggcgtcg ccggtggcgc
    14101 atccgcccag cgcgcggcac tcgcgcaaca aatccggccc cgccgcccgg tgaatcgccc
    14161 cgtcgacccc gcccccgccg agcagcgtcg ggttggcggc gttgacgatg gcttcgacgt
    14221 ccagggtggt gatgtcggcg cggatcgcgc gcacggaggt ggacactacg cggacttcga
    14281 gaatccctcg acgtaccgcg cgagcaggtc cgcttccagg ttcacctcgc tgcccggctt
    14341 caggcgcgcg agcgtagtca ccttcagcgt aaacggaatc aggttcacct cgaactccgc
    14401 gcccttgacg cggttcaccg tcaggctcac gccgtcgatg cagatcgagc ccttgggcgc
    14461 gacgtacttc gccaccgcct tcggtgcgcg gaaggtgtag acgctgccct tcacctgcgt
    14521 caccagcccc acgccatcga cgtgcccgct caccaggtgc ccgccgaggc gatcggcgag
    14581 gcgcagcgcc ttctccaggt tcaccggccc cggcctcgcc aggccggtcg tgcaatccag
    14641 cgtctcctgc gaaacatcga acgaaagccg cttgccgcgc ttcttcgtca ccgtgaggca
    14701 ggcaccctgc acgcagatcg aatcgccgac cttcacgtcg ccaagcggca gcttgccggc
    14761 gtccaccacg aagggtttcg cactgacgat tttcccgacc gcctggacga tgccggagaa
    14821 catcaggccg gcctcgcgag gatgcgcagg tcttcgccga cccgctcgac cgaacggatc
    14881 ttgagcgcga cgcgctggtc gagcgacgcc atgtcctgca ggttgaccat gccctgggcg
    14941 gaatcgccca ggaagctcgg gttcaggtag atcaggaact cgtcgacgca gccctcgcgc
    15001 accagggacc cgttcagctt gaacccgccc tcgacgtgca gctcgttgat gccgcgcctg
    15061 cccagttcct gcagcatttt ctgcaggtcc accttcccgt ccgcattggg cagcaccacg
    15121 atctccgcat tcggcaggga tttgcccttc accgccgcga ataccagcgc cttctcgccc
    15181 tgcaggacct tcgcccgcgc cggcgtctcg acgtggctgt cgatcaccac gcgcacgggt
    15241 tgccgcgggg tctcgacttc ccgcaccgtc agctgcgggt cgtcggtctt caccgtgccg
    15301 ccgcccgtgg cgatggcgca ggcacgcgcc cgccagcggt ggccatcctt cctcgcctcg
    15361 gcgcccgtga tccactgcga tttgccgttg gcgagcgcgg tacgcccgtc cagcgtcgcc
    15421 gcgatcttca tgcgcaccca gggccggccg cgcgtcatgc gcgacacgaa gccgatgttg
    15481 agctcgcgcg c
//
LOCUS       DASRIE010000011         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2092952, whole genome shotgun sequence.
ACCESSION   DASRIE010000011 DASRIE010000000
VERSION     DASRIE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092952"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..2438)
                     /locus_tag="VFB53_00480"
     CDS             complement(30..2438)
                     /locus_tag="VFB53_00480"
                     /inference="COORDINATES: protein motif:HMM:NF045356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85235.1"
                     /translation="MDVARSVLLVVLGAPRSGIGVMARGLQALGARPLQREPGALNLR
                     VLQALGATPASVASLAHADWNSLDALHDEAARLLADPGASAPRTLADASTARLLRFWQ
                     PAFQRAAVEARYVIVHRNPLSVAHALAAQRDAPVERELSHLLWLEHYLEALLGTHGLP
                     RVAIDFDELIDDPAAQLRRACRELELPPPAESAAGLADLLDPRRVHAAFDAQDVAHEA
                     ALAGPARRCHRLLEALSLDAAAADQSGWSQELLEIEAQWKAGALARRRLDGLAQRLHA
                     AERELKHIGKFAEQTQHGAFVAMTHMEQLHRNEQRDLRRVLVDKLRALERVHEVAAAL
                     MREQGSYGARIGRAITRARRRLAPEGSLRWRALTLMVRTAKRLLGRGGAPAAPAPAPA
                     PADAPAYAFVPNPRSLPSVLPLNLATVDALAARPTLNVLLPGLALRAMTGGPNTALNI
                     SYRMAARHGVAVRYISTDAPMQDDRDGLWAHLQSVTGVRERLPLVQFACGFDRSVPLP
                     IGRNDVFLGTAWWTVQMVKHALKLTAPRRFLYLIQEFEPGLYPWSSEYALALETYGLD
                     FHALINQPLVAEHLRAERVGRFADPAFLLERCAVFEPAIERKRFHVEPPAAGRRRRLL
                     FYARPVDARRNLFELALYALRVACEQGVFAGEPWELCFIGEQLPATELVPGVAVRSLA
                     WLGFDEYARTLRQSDVLLSMMLSPHTSYPPLEIAACGGIAVTCEYSVKTAARLAGISP
                     NIVGVPPTVEGIVDGLRRAVQALGAPGTRRPLVRVPESWDDALAEAVPRAVAMFHDCL
                     ATRP"
     gene            2473..3300
                     /locus_tag="VFB53_00485"
     CDS             2473..3300
                     /locus_tag="VFB53_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85236.1"
                     /translation="MNTLARHPSGLAHVFGSIGRHRTLLWQLVAREVAGRYRGSAAGL
                     LWSFFNPLLMLAVYTFVFSVVFRMRWEGASDSRIDFAMLVFAGMIVHGLFAECVNRAP
                     TLVLGSPNLVKKVVFPLEILTWAAVGSALFHAAVSTVVLLAFIAAARHALPWTVILFP
                     LVLAPFALFTAGVTWWLASLGVYVRDVAQVTGVATTVLLFLSPVFFPVSALPEGYRLL
                     FHLNPLTFVIEQAREVLIWGRLPHWPGLALYALLGLAAAWAGLYWFQRTRRGFADVL"
     gene            3290..>3725
                     /locus_tag="VFB53_00490"
     CDS             3290..>3725
                     /locus_tag="VFB53_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85237.1"
                     /translation="MSSEAEGRATDAALSVSALSKCYQIYARPQDRLLQTLWRGRRTF
                     YREFWALRDVSFELARGEAVAVIGRNGSGKSTLLQLIAGTLAPTAGSVRMAGRVSALL
                     ELGSGFNAEFTGRENVVMNAAILGLEGDEIRRRMPEIEAFAGI"
BASE COUNT          487 a         1335 c         1379 g          524 t
ORIGIN      
        1 ggcgcggcgc agcagatgcg cggccgtcct catgggcgcg tagcgaggca atcgtggaac
       61 atggcgaccg cgcgcggcac cgcctcggcg agggcgtcgt cccagctttc cggcacgcgc
      121 accagcggcc ggcgcgtccc cggcgcgccc agcgcctgca ccgcgcggcg caagccgtcg
      181 acgattccct cgaccgtcgg cggcacgccg acgatgttcg gcgaaatgcc ggcgaggcgc
      241 gccgcggtct tcaccgagta ttcgcaggtc accgcgatgc cgccgcaagc cgcgatttcc
      301 agcggcgggt agctggtgtg cggcgacagc atcatcgaca gcagcacgtc gctctgtcgc
      361 agggtgcgcg cgtactcgtc gaagccgagc cacgccagcg agcgcaccgc cacgccaggc
      421 acgagctcgg tcgcgggcag ctgctcgccg atgaagcaca gctcccacgg ctcgccggcg
      481 aacaccccct gctcgcaggc gacgcgcagc gcatacagcg ccagctcgaa caggttgcgg
      541 cgtgcgtcga ccgggcgcgc gtagaacagc aggcgccggc gccggccggc ggccggcggc
      601 tcgacatgga accgcttgcg ctcgatcgcc ggctcgaaca ccgcgcagcg ctcgaggaga
      661 aaggcggggt cggcgaagcg gccgacgcgc tcggcgcgca ggtgctcggc gaccagcggc
      721 tggttgatca gcgcgtggaa atcgaggcca taggtctcga gcgccagcgc gtactcgctc
      781 gaccacggat acaggccggg ctcgaactcc tggatcaggt acaggaagcg ccgcggcgcg
      841 gtcagcttca gcgcgtgctt gaccatctgc acggtccacc acgcggtgcc gaggaacacg
      901 tcgttgcgcc cgatcggcag cggcaccgag cggtcgaagc cgcaggcgaa ctgcaccagc
      961 ggcaggcgct cgcgcacgcc ggtgaccgac tgcaggtgcg cccacaggcc gtcgcggtcg
     1021 tcctgcatcg gcgcgtcggt cgagatgtag cgcacggcga cgccgtggcg ggccgccatg
     1081 cggtaggaga tgttgagcgc ggtgttcggc ccgccggtca tcgcgcgcag cgcgagcccg
     1141 ggcagcagca cgttgagcgt cggccgcgcg gcgagcgcgt cgacggtggc gagattcagc
     1201 ggcagcaccg agggcaggct gcgcgggttc ggcacgaacg cgtaggcagg ggcgtcggcc
     1261 ggtgccggcg cgggcgccgg cgccgccggc gcgccgcccc ggccgagcaa ccgcttcgcc
     1321 gtgcgcacca tgagcgtcaa cgcccgccag cgcagcgagc cctcgggcgc gagccgccgg
     1381 cgcgcgcgcg tgatggcgcg gccgatgcgg gcgccgtagc ttccctgctc gcgcatcagc
     1441 gcggcggcga cttcgtgaac gcgctcgagc gcgcgcagct tgtcgaccag cacgcggcgc
     1501 aagtcccgct gctcgttccg gtgcagctgc tccatgtggg tcatggcgac gaacgcgccg
     1561 tgctgggtct gctcggcgaa cttgccgatg tgcttgagct cgcgctcggc cgcgtgcagg
     1621 cgctgcgcca gcccgtcgag ccggcggcgc gccagggcgc ccgctttcca ttgcgcctcg
     1681 atttcgagca gctcctggct ccatccggac tgatccgcag cggcggcgtc gagcgacagg
     1741 gcttcgagca gccggtggca gcggcgcgcc ggcccggcga gcgcggcttc gtgcgccacg
     1801 tcctgcgcgt cgaacgccgc atgcacccgc cgcggatcga gcaagtctgc gaggcccgcc
     1861 gcggattcgg ccgggggcgg caattcgagc tcgcggcagg cgcggcgcag ctgtgccgcc
     1921 ggatcgtcga tcagctcgtc gaaatcgatc gccacgcgcg gcaggccgtg cgtgccgagc
     1981 agcgcctcga gatagtgctc gagccacagc aggtgcgaca gctcgcgctc gaccggcgcg
     2041 tcgcgttgcg cggcgagcgc gtgcgccacg ctgagcgggt tgcgatggac gatgacgtag
     2101 cgcgcctcga ccgcggcgcg ctggaacgcc ggctgccaga accgcagcag gcgcgcggtg
     2161 gacgcatcgg cgagcgtgcg gggcgcgctt gcgccagggt cggcaagcag gcgcgcggcc
     2221 tcgtcatgca gtgcgtcgag ggagttccaa tctgcgtgcg ccagcgaggc cacgctcgcc
     2281 ggggtcgccc ccagcgcctg cagcaccctg aggttcagcg cgcccggctc gcgctgcagc
     2341 ggccgcgcgc cgagcgcctg caggccgcgc gccatgacgc cgatccccga acggggcgcg
     2401 ccgagcacga cgagcagaac actgcgggcc acgtccatcg gtacggccga ttctacgtag
     2461 aatgcccagc gattgaacac gctcgctcgg catccctcgg gcctcgcgca tgtcttcggc
     2521 tccatcgggc gccatcgcac gctgctgtgg cagctggtcg cgcgcgaggt ggcgggccgc
     2581 taccgcggct cggccgccgg cctgctgtgg tcgttcttca atccgctgct gatgctcgcg
     2641 gtctacacct tcgtgttcag cgtggtgttc cgcatgcgct gggaaggcgc gagcgacagc
     2701 cgcatcgact tcgccatgct ggtgttcgcc gggatgatcg tccacggcct gttcgccgaa
     2761 tgcgtcaacc gcgcgccgac gctggtgctc ggcagcccga acctggtgaa gaaggtggtg
     2821 ttcccgctcg agatcctgac gtgggcggcg gtcggctcgg cgctgttcca cgccgcggtc
     2881 agcaccgtgg tgctgctggc cttcatcgcc gccgcgcgcc acgcgctgcc ctggaccgtg
     2941 atcctgtttc cgctggtgct ggcgccgttc gcgctgttca ccgcgggggt cacgtggtgg
     3001 ctcgcctcgc tcggcgtgta cgtgcgcgac gtcgcgcagg tgaccggcgt cgccaccacg
     3061 gtgctgctgt tcctgagccc ggtgttcttt ccggtgtcgg cgctgccgga gggctaccgg
     3121 ctgctgttcc acctgaaccc gctcaccttc gtgatcgagc aggcgcgcga ggtcctgatc
     3181 tggggccggc tgccgcactg gcccgggctc gcgctctacg cgctgctcgg cctcgccgcg
     3241 gcgtgggccg ggctgtactg gttccagcgc acccgccggg gcttcgccga tgtcctctga
     3301 ggccgaaggc cgggccacgg acgcggcgct cagcgtctcg gcgctgagca agtgctacca
     3361 gatctacgcc cgcccgcagg accgcctgct gcagaccctg tggcgcgggc ggcgcacctt
     3421 ctaccgcgag ttctgggcgc tgcgcgacgt gagcttcgag ctcgcgcgcg gcgaggcggt
     3481 ggcggtcatc ggccgcaacg gctcgggcaa gagcacgctg ctgcagctga tcgccggcac
     3541 gctcgcgccg accgcgggct cggtgcgcat ggcggggcgc gtctcggcgc tgctcgagct
     3601 cggcagcggc ttcaacgccg agttcaccgg gcgcgagaac gtggtgatga acgccgcgat
     3661 cctcggactc gagggcgacg agatccggcg gcgcatgccc gagatcgagg cgttcgccgg
     3721 catcg
//
LOCUS       DASRIE010000012        13897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2354900, whole genome shotgun sequence.
ACCESSION   DASRIE010000012 DASRIE010000000
VERSION     DASRIE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354900"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFB53_00495"
     CDS             complement(<1..674)
                     /locus_tag="VFB53_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN85238.1"
                     /translation="MDQKPTRAIVAAVQLPGASDVEFESSLAELRQLAKTLGFEVVGT
                     LTQKRARLDAEAYLGTGKREELRALAQAGAEVVLVDHEISPSQAHNLAREIGCEVMDR
                     SMVILEIFHRHARSAAARAQVEIARLGYMGPRLREAAKQAGPKDRARSGTGGRSGGES
                     HGELDRRKIRDRIAELQKELDAMTLGRSTQRARRQERQGLARVALVGYTNAGKSTLMR
                     ALTGSEV"
     gene            complement(685..3363)
                     /locus_tag="VFB53_00500"
     CDS             complement(685..3363)
                     /locus_tag="VFB53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85239.1"
                     /translation="MTRQLLREPFRLPLAFFASGMAALLFQVLWFRAFGRVLGNTVWA
                     GALVLTAFMLGVAIGGLLAARWAQRIRNAARAFALAEVTVAVTGSLLVWGLPHLEAVV
                     GQWLAPLAGHPAAIAGLRLALALGAMLVPTIAMGTTLALGVRVLARRETTRALGVLYA
                     ANTFGACLAPLLAEYYLIGALGLRSTALVAAGLNALAAVLALAQPPGAAAAPAPATGA
                     RSFPRRLLFAAAAAGGLALALEVIWFRLLVLYAPGSDTSFALMLTVVLLGIALGGALA
                     PLLARLQLVWITMGSSLAVVLGYLLAGWTFSSGKPDLVHIAVALMLPAAMLSGLLFTL
                     LGAELRADADDPQPAIGVLTTANTLGAAGGAALAGLVLLPQLGIEWSLFALAAGYALL
                     PLLVAPAARMALPLAAAAAGLLMFPFGRMDMHLAEAALPYQLNDGSRVVQVTQGPTIT
                     LQLLRRERFGEAAAWRLLTDSYSMTAINHQALRYMQMFAWLPLALHPEPRRALLISYG
                     AGNTAQALLSEPQLRRLTVVDVSPEILGASALVHGKRNPLQDPRVQLVLEDGRHYLRL
                     HREQFDIITAEPPPPLIAGVVNLYTREYFTAIAGRLAPGGLATYWLPAPQFKPQGARA
                     VVRAFCDAFPDCTLWAGDQRNWILMGGREFRHRPSAQHFARLWRGAASAPRIAANGIE
                     HPVQLGATFLADAGELRRWAGATPALTDDHPKRIAPETDVERPVDEYAAWLKPEDAQR
                     RFRESPWVARHWPAELTQASLPFFAINPLFNGGTSTDHIRNLPQVDAILRRTDLRIPV
                     LWLLGSDVAEQEIVNRRLAAGGYRPDYAYALGVRALAERDYATAAELFAEAAGRGPGP
                     AGALAAYSLCRAGLRQKAAAVAGADKLAPALRCWSH"
     gene            complement(3365..4255)
                     /locus_tag="VFB53_00505"
     CDS             complement(3365..4255)
                     /locus_tag="VFB53_00505"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN85240.1"
                     /translation="MSPWQADRLLPGFESREFGFPEDYDGRVVSTLVRLPAGDAPRGA
                     VLYVHGFIDYFFQRHMAERFAAEGYAFHALDLRKHGRSLLAHQHPCFCKDVAEYHADI
                     SRALEAIDAPVLLAGHSTGGLVCALYAAQGERREQLRALWLNSPFFDWRVSAFKRAQL
                     GIAALAGQAFPFLNDPKAVRPEYVRSIHREFGGEWDFDLKLKPLYGFPAYFGWIGAIL
                     AAQAKVHAGLAIRCPVLTMHSDEADIVLDWRHLARWGRTLGGAATVLAFPGGLHDLVL
                     SRQEIRDAVFGELFSWAART"
     gene            complement(4252..6237)
                     /locus_tag="VFB53_00510"
     CDS             complement(4252..6237)
                     /locus_tag="VFB53_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZN85241.1"
                     /translation="MNAEPHAAPAEIEALRARSLGRAWRALLIAAAAATIFLCVNQQF
                     VLRFFVGFTPLNTEYYYALVLVMLPFVFVIFPGGERARMDGVPWYDAALFAITLATSA
                     YLLAHIRNAAELGWEFGGAPAGVVWAGYAMWALLMEGLRRTGGWGLVLSVLPFTLYPL
                     FADSAWLGPLKGTQSTAAQTSAYHMLSIESLLGIPLQAFAEVVIGFLVFGTALMTTGA
                     GRFFINLSFSLCGTFRGGAAKVCVFASALLGMMSGSIVSNVLTAGTMTIPVMKKTGFR
                     ASYAGAIEACASTGAVLAPPVMGATAFVMAQFLNLSYAEVALAAAIPAALYYFGLFMQ
                     VDAYAARHGLKGLPREELPRLWDTVKEGWYFVLVIVLLVYMMLVMKRESHAPFYATVL
                     LLVLNQIFNRERWTWASAAKFLELNGRTFAELVGILAGCGLLIGAFSLTGVISSLAND
                     MLRIAGSNAFLLLSMTALTSLVLGLGLTTTACYIFLAILVGPALEKVGLNKLAVHMFV
                     FYWGMLSSITPPVAIASFAAAGIAGSPPMQTAWESMRVGSIIYFIPFFFVLNPAFLLQ
                     GPLAETLAVTAACALGIVFICGGIQGYQMWTGDLRRAGAAEWPLRALLILGGMVLAAP
                     GGGLMPLSSVQMAMLGAAILLPTLAAAWILARRLRAA"
     gene            complement(6256..7413)
                     /locus_tag="VFB53_00515"
     CDS             complement(6256..7413)
                     /locus_tag="VFB53_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZN85242.1"
                     /translation="MAKHAFVQGAALLVSAALAAGASAQGIKLPATLTVTAYDTGSSG
                     FNIAVAVGKAMKDRHGTDVRVLPAGNDVARLAPLKGGRAQASYMGIGVYFAQEGVFEF
                     AARDWGPQPLRLIVASSDCNAISLGVAKDTGVKQIRDLKGKRIGMVVGSPALNQNAFA
                     VLAFGGLTRSDVKLVEFSSYGAMWKGILNNEVDAAIASTISGQAKEAEASPRGLVYPP
                     TPAADKAGWTRMHKFGPYYAPHTATCGVGITPQTPVELPNYPYPIVTVYDSQAADLVY
                     GIARSMIADYPAYKDGAPGAAGLELARQNLTWVLPYHEGAVRAFKEAGVWKAEHEAHQ
                     QKALARQSTLGAAWSAFLKTNPPEDRDALRKGWMAARGAALQKAGFDAIFE"
     gene            complement(7434..8510)
                     /gene="queG"
                     /locus_tag="VFB53_00520"
     CDS             complement(7434..8510)
                     /gene="queG"
                     /locus_tag="VFB53_00520"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZN85243.1"
                     /translation="MDYAALAARIKQWGRELGFQAVGIADTELAAAEVRLGEWLGKGW
                     HGEMDYMARHGALRARPAELVPGTLRVISCRMDYLPRADEPAEDPLLAAIARYARGRD
                     YHKLVRGRLQKLCDRIEAEAGAFGHRVFADSAPVMEVALAEKAGIGWRGKHTLLLSRE
                     AGSWFFLGEIYASLPLPADAPALEHCGSCVKCIEVCPTQAIRGPYQLDARRCISYLTI
                     ELKGSIPEALRPLIGNRVYGCDDCQAVCPWNSFARPSAEPDFAPRHGLDRATLVELFA
                     WSAAEFDARMAGSPIRRIGHERWLRNLAVGLGNAPRAPEVLAALRARADHPSALVREH
                     VAWALERHGEARQNAAATAPSGSP"
     gene            8509..8991
                     /gene="tsaE"
                     /locus_tag="VFB53_00525"
     CDS             8509..8991
                     /gene="tsaE"
                     /locus_tag="VFB53_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZN85244.1"
                     /translation="MAAESSLELAGEADTLRLGAALSVGVGPGRTLTLQGDLGTGKTT
                     LVRGLLRALGHAGPVKSPSYPLVELYVVSRLHLYHFDFYRIKDRTEWRDSGFRDYFNA
                     QSACVVEWPERAGGLLAPPDLAVRLEIAGAGRRAHLSAGTPAGAGWLAQARQRYSSSA
                     "
     gene            8949..10160
                     /locus_tag="VFB53_00530"
     CDS             8949..10160
                     /locus_tag="VFB53_00530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN85245.1"
                     /translation="MAGAGAAALLVLGLGAAQAAELIATRIWPARDYTRVTFESRQEL
                     NYSMFALKGPDRLVLDLAGADPGPALAELQNKVAAGDPYIASLRVAQNRPGVMRVVLD
                     LKTEVKPQVFALKPIADYGHRLVLDLYPLVPLDPLLALYERDSRPAPAPRKGAGATLP
                     GKPDVARLATIVIDAGHGGEDPGARGRRGSLEKHITLTIARRLKTMIDAESGMRAVLT
                     RDGDYYLPLNARVEKARKVRADLFVSVHADAFVRPHARGSSVFALSQRPTSDAARWLA
                     KQENDSDLIGGVNLDARDPYLTQTLVDLSQTATNDHSLKLGGAVLAELGLINTLHKAR
                     VERAGFAVLKAPDVPSILVETAFISNPEEEKRLNDEAYQDKMARALLAGIKRYFARHP
                     PRPQGPLAALR"
     gene            complement(10173..10811)
                     /locus_tag="VFB53_00535"
     CDS             complement(10173..10811)
                     /locus_tag="VFB53_00535"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZN85246.1"
                     /translation="MELFALLWDLTVNLDDHLAAFVAQHGVWVYALLFAVVFTETGVV
                     VMPFLPGDSLLFVTGALAAAGGMDIVTVMGVLVAAALTGDNTNYWIGRWAGPKVFRAE
                     SSPWFSRRHLERTHAFYERHGGKAIILARFVPIMRTYVPFVAGIGTMTYARFLGFSVL
                     GALLWVVSLCLAGYWFGNLPLVKDNLGFVILAIIAVSVSPIVVEYLRGRRRA"
     gene            10836..12533
                     /gene="mutL"
                     /locus_tag="VFB53_00540"
     CDS             10836..12533
                     /gene="mutL"
                     /locus_tag="VFB53_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZN85247.1"
                     /translation="MRAIRVLPELLIGQIAAGEVVERPASVLKELLENSLDAGAREIQ
                     VTLAEGGAKRVQVDDDGEGIARADLALAFARHATSKIGTLQDLEAVASMGFRGEALAS
                     IAAVARVEIVSRARGEARAWSLSAEGGAPGEARPAARAPGTTVAVADLYFNTPARRKF
                     LRTEATEFGHCDEVFRRVALARPDLAFTLKHNGRVSAQLAPQPLEARAAALLGEEFGA
                     ASVPLDARAGPLRLHGLAGTPQAARNRADAQYFYVNGRYVRDQLLAHAVREAYRDTLH
                     GERQPAFALFLELDPRGVDVNVHPAKTEVRFRDARGMHQFVFHALKRALSPSAAESPV
                     SYATVSAPGPAIQAALAMQEPVPEYRPAAVPLPASAQAPPLGYALAQLHGVYILAQNE
                     AGLVLVDMHAAHERILMEKLKLNLDAGAVERQSLLVPAVFRAEALDVAAAEEHRAALE
                     KLGLELSVAGPNELAVRAAPALLAGGDVAALARSLLAELREFGAAQVLAARQNELLGT
                     MACHAAVRANRVLSVHEMNALLREMEETERSGSCNHGRPTWYQLSMADLDKLFLRGR"
     gene            12523..13419
                     /gene="miaA"
                     /locus_tag="VFB53_00545"
     CDS             12523..13419
                     /gene="miaA"
                     /locus_tag="VFB53_00545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZN85248.1"
                     /translation="MAARAYALLGPTASGKSTLAMALAAEFPLEIVSMDSAQVYRGMD
                     VGTAKPSLAERARIRHHLVDIVEPTRSYSAGRFRADAVRAIGEIQGRGKVPLIVGGSM
                     LYFKALSQGIDELPVAEPGLRAQIDARAARHGWPALHADLAKVDPVTAARLAPNDAQR
                     IQRALEVWELTGKPISELQRAAGASLPFELKSFALVPADRGELHRRIAQRFERMLKDG
                     LIEELKGLRRQHELHAALPSMRCVGYRQAWEFLEGEYGKAELRERGIAATRQLAKRQL
                     TWLRSLPGLEPAERLAAALTRA"
     gene            complement(13406..>13897)
                     /locus_tag="VFB53_00550"
     CDS             complement(13406..>13897)
                     /locus_tag="VFB53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85249.1"
                     /translation="NSTAMLSTAQLVQQEKLDVVHIPYKGDGPLFVDLLGGRVQFAIS
                     TPGAAAPQVKEGRLKVLATLLPSRSPLLPQAPTMAEAGLRGLSITPWGGVFGPKGMPR
                     DITERLSRELIALLKRPEVREAFEKLAFEPHGSTPEELAAFTREQVAVWRRVVGEVGI
                     KPE"
BASE COUNT         2088 a         4991 c         4997 g         1821 t
ORIGIN      
        1 acctcgcttc ccgtcagggc gcgcatcagg gtggacttgc cggcgttggt gtagccgacc
       61 agcgccacgc gcgcgaggcc ctggcgctcc tggcgccgcg cgcgctgcgt gctgcgcccg
      121 agggtcatcg cgtccagttc cttctgcagc tcggcgatgc ggtcgcggat cttgcgccgg
      181 tccagctcgc cgtgcgattc gccgccgctc ctgccgccgg tgccgctgcg cgcccggtcc
      241 ttgggcccgg cctgcttggc ggcctcgcgc aggcgcggcc ccatgtagcc caggcgcgcg
      301 atctccacct gcgcgcgcgc ggccgccgag cgcgcgtgcc ggtggaagat ctcgaggatc
      361 accatgctgc ggtccatcac ctcgcagccg atttccctgg cgaggttgtg cgcctgcgac
      421 ggggagatct cgtggtcgac caggaccacc tcggcgcccg cctgcgccag ggcgcgcagc
      481 tcctcgcgct tgcccgtccc gaggtacgcc tccgcatcca gcctggcgcg cttctgcgtg
      541 agcgtgccga cgacctcgaa gcccagcgtc ttggcgagct ggcgcagctc ggcgagcgag
      601 gactcgaact cgacgtcgct cgcgccgggc agctgcacgg ccgccacgat ggcgcgcgtc
      661 ggtttctggt ccaccacccg catcctagtg gctccagcag cgcagcgcgg gcgcgagctt
      721 gtcggcgcct gccaccgccg ccgccttctg gcgcagcccc gcacggcaca gggaataggc
      781 ggcgagcgcg ccggccgggc cgggcccgcg gccggcggcc tcggcgaaaa gctccgccgc
      841 ggtggcgtag tcgcgctccg caagcgcgcg cacgccgagc gcgtaggcgt agtccgggcg
      901 atagcccccg gccgccagcc gccggttcac gatctcctgc tcggcgacgt cgctgccgag
      961 cagccacagg accgggatgc gcaggtcggt ccggcgcagg atcgcgtcca cctgcggcag
     1021 gttgcggatg tggtcggtgg acgtcccgcc gttgaacagc gggttgatcg cgaagaaagg
     1081 caggctcgcc tgggtcaact cggcgggcca gtggcgcgcc acccacggcg actcccggaa
     1141 gcggcgctgc gcgtcttcgg gcttgagcca ggccgcgtat tcgtccaccg ggcgctcgac
     1201 gtcggtttcc ggcgcgatgc gcttcggatg gtcgtcggtc aaggccggcg tcgcgccggc
     1261 ccagcggcgc agctccccgg cgtccgcgag gaaggtcgcg ccgagctgca ccgggtgctc
     1321 gatcccgttc gcggcgatgc gcggcgccga cgccgcgccg cgccacaggc gggcgaaatg
     1381 ctgcgcgctc gggcgatgcc tgaactcgcg gccgcccatc aggatccagt tgcgctggtc
     1441 gccggcccac agcgtgcaat cgggaaacgc gtcgcagaag gcgcgcacca ccgcgcgcgc
     1501 cccctgcggc ttgaattgcg gcgccggcag ccagtaggtc gcgagcccgc cgggggcgag
     1561 ccggccggcg atcgccgtga agtactcgcg ggtatacagg ttcaccacgc ccgcgatcag
     1621 cggcggcggc ggctccgcgg tgatgatgtc gaactgctcg cgatgcaggc gcaggtagtg
     1681 gcgcccgtct tccaggacca gctgcacccg cgggtcctgc agcggattgc gcttgccgtg
     1741 caccagcgcg ctcgcgccga ggatctccgg cgagacgtcg accacggtga gccggcgcag
     1801 ctgcggctcg ctcaacagcg cctgcgcggt gttgcccgcg ccatagctga tcagcagcgc
     1861 gcgccgcggc tcgggatgca gcgcgagcgg cagccaggcg aacatctgca tgtagcgcag
     1921 cgcctggtga ttgatcgccg tcatcgaata gctgtcggtc agcagccgcc aggccgccgc
     1981 ctcgccgaag cgctcgcgcc tgagcagctg cagggtgatc gtcgggccct gcgtgacctg
     2041 cacgaccctg gagccgtcgt tcagctggta cggcagcgcc gcctccgcca ggtgcatgtc
     2101 catgcgcccg aacggaaaca tcagcaggcc ggccgccgcc gcggcaagcg gcagcgccat
     2161 gcgcgcggcc ggcgcgacga ggagcggcag cagcgcatag cccgcggcga gcgcgaacag
     2221 gctccattcg atgcccagct gcggcagcag caccaggccc gccaacgccg cgccgcctgc
     2281 ggcgccgagc gtattcgcgg tggtgagcac gccgatcgcg ggctgcgggt cgtccgcgtc
     2341 ggcgcgcagc tcggcgccca gcagcgtgaa caacaggccc gacagcatcg ccgccggcag
     2401 catcaaggcg accgcgatgt ggaccaggtc cggcttgccc gagctgaaag tccagccggc
     2461 gagcaggtag ccgagcacga cggcgaggct gctgcccatg gtgatccaga ccagctgcag
     2521 ccgggcaagc agcggggcga gcgcgccgcc gagcgcgatg ccgagcagga ccacggtcag
     2581 catgagcgcg aagctcgtat cgctgcccgg cgcatagagg accagcaggc ggaaccagat
     2641 cacttcgagc gcgagcgcga ggccccccgc cgcggcggcg gcgaacagca ggcgtcgcgg
     2701 gaaggaccgg gcgccggtcg cgggcgccgg cgcggcggcg gcgcccggcg gctgcgccag
     2761 ggcgagcacc gccgccagtg cattgaggcc cgcggcgacc agcgccgtgc tgcgcagtcc
     2821 gagcgcgccg atcaggtaat actcggcgag gagcggcgcg aggcaggcgc cgaaggtgtt
     2881 cgccgcatac agcacgccga gcgcgcgcgt ggtttccctg cgcgccagga cgcgcacgcc
     2941 gagcgcgagc gtcgtcccca tcgcgatcgt cggcacgagc atggcgccga gggcgagcgc
     3001 gaggcgcagg cccgcgatcg ccgcgggatg gccggcgagc ggcgcgagcc actgcccgac
     3061 caccgcttcc agatgcggga gcccccagac cagcaggctt cccgtgaccg ccaccgtgac
     3121 ctcggcgagc gcgaacgcgc gcgcggcatt gcggatgcgc tgcgcccagc gcgcggcgag
     3181 caggccgccg atcgccacgc cgagcatgaa cgcggtgagg accagcgcgc cggcccagac
     3241 cgtgttgccg agcacgcggc cgaacgcgcg gaaccacagc acctggaaca ggagcgccgc
     3301 catgcccgag gcgaaaaagg cgagcggcag ccggaaaggt tcgcgcagca gctgtcgcgt
     3361 cacgctaggt ccgtgcggcc caggaaaaca gctccccgaa caccgcgtcg cggatctcct
     3421 gccgcgacag caccaggtcg tgcagcccgc ccgggaaggc gagcaccgtc gccgcgccgc
     3481 ccagcgtgcg gccccagcgc gccaggtggc gccagtcgag cacgatgtcc gcctcgtcgg
     3541 agtgcatggt gagcaccggg cagcggatgg ccagccccgc gtgcaccttc gcctgcgcgg
     3601 cgaggatggc gccgatccag ccgaaatagg cgggaaaacc gtacaggggc ttgagcttca
     3661 ggtcgaagtc ccattcgccg ccgaactcgc ggtggatgct gcgcacgtac tcgggacgga
     3721 ccgccttggg atcgttgagg aaggggaatg cctgtccggc gagcgcggcg atgccgagct
     3781 gcgcgcgctt gaaggcagag acccgccagt cgaagaacgg gctgttcagc cacagggcgc
     3841 gcagctgctc gcggcgctct ccctgcgcgg catacagcgc gcagaccagc cccccggtgg
     3901 agtgcccggc gagcagcacc ggcgcgtcga tcgcctccag cgcgcggctg atgtccgcgt
     3961 ggtactcggc aacgtccttg cagaagcagg gatgctggtg cgcgaggagc gagcggccgt
     4021 gcttcctcag gtcgagggca tggaaggcgt agccctcggc ggcgaagcgc tcggccatgt
     4081 ggcgctggaa aaaatagtcg atgaagccgt gcacgtagag caccgcgccg cgcggcgcgt
     4141 cgcccgcggg caggcgcacc agggtcgaca ccacgcgccc gtcgtagtcc tccgggaatc
     4201 cgaattcgcg cgactcgaac cccggcagca gccggtcggc ctgccagggg ctcatgcggc
     4261 gcgcaggcgg cgcgccagaa tccaggccgc cgccagcgtc ggcagcagga tcgccgctcc
     4321 cagcatcgcc atctgcacgc tggacagcgg catcagcccg ccgcccgggg ccgccagcac
     4381 catgccgccc aggatcagca gcgcgcgcag cggccattcc gcggccccgg cgcgccgcag
     4441 gtcgcccgtc cacatctggt agccctggat gccgccgcag atgaacacga tgcccagcgc
     4501 gcaggcggcg gtgacggcca gggtctcggc gagcggaccc tgcagcagga acgccgggtt
     4561 gagcacgaag aagaacggga tgaaatagat gatgctgccc acgcgcatcg attcccaggc
     4621 ggtctgcatc ggcggcgagc cggcgatgcc ggcggcggcg aacgaggcga tggccaccgg
     4681 cggcgtgatc gaggacagca tgccccagta gaacacgaac atgtgcacgg cgagcttgtt
     4741 cagccccacc ttctccagcg ccggccccac caggatggcg aggaagatgt agcaggcggt
     4801 ggtggtgagg cccaggccca gcaccaggct ggtcagcgcc gtcatcgaca gcagcaggaa
     4861 cgcgttgctg cccgcgatgc gcagcatgtc gttggccagg ctcgagatca cgccggtgag
     4921 cgagaacgcg ccgatcagga ggccgcagcc ggcgaggatg ccgaccagct cggcgaaggt
     4981 gcggccgttc agctcgagga atttcgccgc gctggcccag gtccagcgct cgcggttgaa
     5041 gatctggttc agcaccagca gcagcaccgt ggcatagaac ggcgcatggc tctcgcgctt
     5101 catcaccagc atcatgtaca ccagcaggac gatcaccagc acgaagtacc agccctcctt
     5161 caccgtgtcc cagaggcgcg gcagctcttc gcgcggcagg cccttcaggc cgtggcgcgc
     5221 ggcgtaggcg tccacctgca tgaacaggcc gaagtagtac agcgccgcgg gaatcgcggc
     5281 ggcgagcgcc acctcggcgt agctcaggtt gaggaattgc gccatcacga aggcggtggc
     5341 gcccatcacc gggggcgcca gcaccgcgcc ggtggaggcg caggcctcga tggcgccggc
     5401 gtagctggcg cggaagcccg tcttcttcat caccgggatg gtcatggtgc cggcggtgag
     5461 cacgttgctg acgatcgagc ccgacatcat gccgagcagc gcgctggcga acacgcagac
     5521 cttggcggcg ccgccgcgga aggtgccgca cagcgagaac gacaggttga tgaagaagcg
     5581 cccggcgccg gtggtcatca gcgccgtgcc gaagacgagg aagccgatca ccacctcggc
     5641 gaaggcctgc agggggatgc cgagcaggct ctcgatcgac agcatgtggt aggcgctggt
     5701 ctgcgccgcc gtggattgcg tgcccttgag cggcccgagc caggcggaat cggcgaacag
     5761 cgggtacagc gtgaagggca gcaccgagag caccaggccc cagccgccgg tgcgccgcag
     5821 gccttccatc agcagcgccc acatcgcgta gcccgcccac accacgcccg ccggggcgcc
     5881 gccgaactcc cagcccagtt ccgcggcgtt gcggatgtgc gcgagcaggt aggccgaggt
     5941 cgccagcgtg atcgcgaaca gcgccgcgtc gtaccagggc accccgtcca tgcgcgcgcg
     6001 ctccccgccg gggaagatca cgaacacgaa cggcagcatc accagcacca gcgcgtagta
     6061 gtactcggtg ttgagcgggg tgaagccgac gaagaagcgc aggacgaact gctggttgac
     6121 gcagaggaag atggtggccg ccgccgccgc gatcagcagc gcgcgccacg cgcgcccgag
     6181 ggagcgcgcg cgcagcgcct cgatttccgc gggcgcggcg tgcggttcgg cgttcacgcc
     6241 gcgcgccgtg cgccgctatt cgaagatggc gtcgaagccg gctttttgca gcgcggcgcc
     6301 gcgcgccgcc atccagccct tgcgcagcgc gtcgcggtcc tcgggcggat tggtcttgag
     6361 gaaggcgctc caggcggcgc ccagcgtcga ctgccgcgcg agcgcctttt gctggtgcgc
     6421 ctcgtgctcg gccttccaca cgccggcctc cttgaaggcg cgcaccgctc cctcgtggta
     6481 gggcaggacc caggtgaggt tctggcgcgc gagctccagc cccgccgccc ccggcgcgcc
     6541 gtccttgtac gccggatagt ccgcgatcat cgacctggcg atgccgtaga cgaggtcggc
     6601 ggcctgcgag tcgtagaccg tgacgatcgg atagggatag ttcggcagct cgaccggggt
     6661 ttgcggcgtg atgcccacgc cgcaggtggc ggtgtgcggc gcgtagtagg ggccgaactt
     6721 gtgcatgcgg gtccagccgg ccttgtcggc ggccggcgtg ggcggataca ccagcccgcg
     6781 cggcgaggcc tccgcctcct tcgcctgccc cgaaatggtg gaggcgatgg ccgcgtccac
     6841 ttcgttgttg aggatcccct tccacatcgc gccgtagctg gagaactcga ccagcttcac
     6901 gtcgctgcgc gtcaagccgc cgaaggcgag caccgcaaag gcgttctggt tgagcgcggg
     6961 cgagcccacc accatgccga tgcgcttgcc cttgagatcc ctgatctgct tgacgccggt
     7021 gtccttggcc acgccgagcg agatggcgtt gcaatcgctc gaggcgacga tcagccgcag
     7081 cggctgcggg ccccagtccc tggccgcgaa ctcgaacacg ccttcctgcg cgaagtacac
     7141 gccgatgccc atgtaggaag cctgcgcgcg gccgcccttc agcggcgcca ggcgggcgac
     7201 gtcgttgccc gcgggcagga cgcgcacgtc ggtgccgtgc cggtccttca tcgccttgcc
     7261 caccgcgacc gcgatgttga atccggacga gccggtgtcg taggccgtga cggtcagcgt
     7321 ggccgggagc ttgatgccct gcgccgacgc gccggcggcc agggccgccg acaccagcaa
     7381 cgcggcgccc tgcacgaatg catgttttgc catgattccc tcctccaggg tatttacggc
     7441 gaacccgatg gtgccgtggc cgcggcgttc tgtcgagcct cgccgtggcg ctcgagcgcc
     7501 caggccacgt gttcccgcac cagggcggaa gggtgatcgg cccgcgcgcg cagcgcagcg
     7561 aggacctcgg gcgcgcgcgg cgcgttgccc aggccgacgg cgagattgcg cagccagcgc
     7621 tcgtggccga tgcgccggat cggcgagccg gccatgcgcg cgtcgaactc ggccgcgctc
     7681 caggcgaaca gttccaccag cgtcgcgcgg tccagcccgt ggcgcggcgc gaaatcgggc
     7741 tcggcggagg gccgggcgaa ggagttccac gggcacaccg cctggcagtc gtcgcagccg
     7801 tagacgcggt tgccgatgag cggccgcagc gcttcgggga tgctgccctt caattcgatg
     7861 gtgaggtagg aaatgcagcg ccgcgcgtcg agctggtagg ggccgcggat ggcctgcgtc
     7921 gggcacacct cgatgcattt cacgcagctg ccgcagtgct cgagcgccgg cgcgtcggcg
     7981 ggcagcggca ggctggcgta gatctcgccc aggaaaaacc aggagcccgc ctcgcgcgac
     8041 agcagcagcg tgtgcttgcc gcgccagccg atgcccgcct tctccgccag cgccacttcc
     8101 attaccggcg ccgagtcggc gaacacgcga tggccgaagg cgccggcctc ggcctcgatg
     8161 cggtcgcaca gcttctgcag gcggccgcgc acgagcttgt ggtagtcgcg tccgcgcgcg
     8221 tagcgcgcga tcgccgcgag cagcggatcc tccgcgggtt cgtccgcgcg cggcaggtag
     8281 tccatgcggc aggagatcac gcgcagcgtg cccggcacca gctccgccgg gcgcgcgcgg
     8341 agcgcgccgt ggcgcgccat ataatccatc tcgccgtgcc agcccttgcc gagccactcg
     8401 ccgagccgga cttccgccgc ggcgagctcg gtgtccgcga tgccgaccgc ctggaagccg
     8461 agctccctgc cccactgctt gatgcgcgcc gcgagcgccg cgtaatccat ggccgccgag
     8521 tctagcctcg agctcgccgg cgaggcggac acgctgcgcc tgggggcggc gctgtctgtc
     8581 ggcgtcgggc cgggccggac gttaaccctg cagggggacc tgggaaccgg caaaacgacc
     8641 ctggtccggg ggttgctgcg cgcgctgggc cacgctggcc cggtcaagag ccccagctac
     8701 cccctggttg aactttatgt ggtttcaaga ttacacttgt atcactttga tttttatcga
     8761 atcaaagatc ggacggaatg gcgcgattct ggattcaggg attacttcaa cgctcagtcg
     8821 gcctgcgtgg tcgagtggcc cgagcgcgcg ggcggcctgc tcgccccgcc ggacctggcg
     8881 gtgcgcctgg aaatcgcggg ggcgggacgg cgcgcgcacc tgagcgccgg cacgcctgcc
     8941 ggcgccggct ggctggcgca ggcgcggcag cgctactcgt cctcggccta ggcgcggcgc
     9001 aggccgccga gctcatcgcc acgcgcatct ggccggcgcg cgactacacg cgcgtgacct
     9061 tcgaatcgcg ccaggaactg aactacagca tgttcgcgct caagggcccc gaccgcctgg
     9121 tgctcgacct ggcgggcgcc gatcccggcc ccgcgctcgc ggagctgcag aacaaggtcg
     9181 ccgccggcga cccctacatc gccagcctgc gcgtggcgca gaaccggccc ggcgtgatgc
     9241 gcgtggtgct cgacctgaag accgaggtga agccgcaggt gttcgcgctc aagccgatcg
     9301 ccgactacgg ccaccggctg gtgctcgacc tgtacccgct ggtgccgctc gacccgctgc
     9361 tcgccctgta cgagcgcgac agcaggcccg cgccggcgcc gcgcaagggc gcgggggcga
     9421 ccctgcccgg caagcccgac gtcgcgcgcc tcgccaccat cgtgatcgac gccgggcacg
     9481 gcggcgagga cccgggcgcg cgcggccgcc gcggcagcct ggaaaagcac atcaccctga
     9541 ccatcgcgcg ccgcctgaag accatgatcg acgccgaatc cggcatgcgc gcggtgctga
     9601 cgcgcgacgg cgactactac ctgccgctca acgcgcgcgt ggagaaggcg cgcaaggtgc
     9661 gcgcggacct gttcgtctcg gtgcacgccg acgccttcgt gcggccgcac gcgcgcggct
     9721 cctcggtgtt cgcgctgtcg cagcgcccga cctcggacgc cgcgcgctgg ctggccaagc
     9781 aggaaaacga ttccgacctg atcggcggcg tgaacctgga tgcgcgcgac ccgtacctga
     9841 cgcagaccct ggtcgacctg tcgcagaccg cgaccaacga ccacagcctg aagctgggcg
     9901 gcgcggtgct ggccgagctg gggctgatca acaccctgca caaggcgcgc gtcgagcgcg
     9961 ccggcttcgc cgtgctcaag gccccggacg tgccctcgat cctggtggag accgcgttca
    10021 tctccaaccc ggaggaggaa aagcgcctca acgacgaggc ctaccaggac aagatggcgc
    10081 gggcgctgct cgccggcatc aagcgctatt tcgcccggca cccgccgcgg ccgcaggggc
    10141 cgctcgccgc cctgcgctag gcgcgttcgc gctcaggcgc ggcggcggcc gcgcagatac
    10201 tccaccacga tcgggctgac cgacaccgcg atgatcgcga ggatcacgaa gcccaggttg
    10261 tccttcacca gcggcaggtt gccgaaccag tagccggcga ggcacagcga caccacccag
    10321 agcagcgcgc cgagcacgct gaagccgagg aagcgcgcgt aggtcatggt gccgatgccc
    10381 gccacgaagg gcacgtaggt gcgcatgatc ggcacgaagc gcgcgaggat gatcgccttg
    10441 ccgccgtggc gctcgtagaa cgcgtgcgtg cgctcgaggt gcctgcggct gaaccagggc
    10501 gagctttcgg cgcggaacac cttggggccc gcccagcgcc cgatccagta gttggtgttg
    10561 tcgccggtga gcgcggccgc cacgagcacg cccatcaccg tgacgatgtc catgccgccc
    10621 gcggcggcga gcgcgccggt gacgaacagc agcgaatcgc cgggcaggaa cggcatcacc
    10681 accaccccgg tctcggtgaa cacgacggcg aacaacagcg cgtagaccca gacgccatgc
    10741 tgcgcgacga aggccgccag atggtcgtcg agattgaccg tgaggtccca gagcagggcg
    10801 aaaagttcca ccacccgatt ctatatactc cccgcatgcg cgccatccgc gtcctgcccg
    10861 agctgctgat cggccagatc gccgccggcg aggtggtcga gcggcccgcc tcggtgctga
    10921 aggagctgct ggagaacagc ctcgacgcgg gcgcgcgcga gatccaggtg acgctggccg
    10981 agggcggcgc gaagcgcgtg caggtggacg acgacggcga gggcatcgcg cgcgcggacc
    11041 tggcgctcgc cttcgcgcgc cacgccacca gcaagatcgg cacgctgcag gacctcgagg
    11101 cggtggccag catgggtttt cgcggcgagg cgctcgcctc gatcgccgcg gtggcgcgcg
    11161 tcgagatcgt ctcgcgcgcc cggggagaag cgcgtgcctg gtcgctctcg gccgaaggcg
    11221 gcgcgccggg cgaagcgcgc cccgcggcgc gcgcgccggg caccacggtg gcggtggccg
    11281 acctctactt caacactccg gcgcggcgca agttcctgcg caccgaggcc accgagttcg
    11341 gccactgcga cgaggtgttc cggcgcgtcg cgctggcgcg cccggacctc gccttcacgc
    11401 tgaagcataa cggccgcgtc tcggcgcagc ttgcgccgca gccgctcgag gcgcgcgccg
    11461 cggcgctgct cggcgaggag ttcggcgccg cctcggtgcc gctcgacgcg cgcgcgggcc
    11521 ccttgcgcct gcacggcctc gccggcacgc cgcaggcggc gcgcaaccgc gccgacgcgc
    11581 agtatttcta cgtcaacggc cgctacgtgc gcgaccagct gctcgcgcac gcggtgcgcg
    11641 aggcctaccg cgacaccctg cacggcgagc gccagccggc cttcgcgctg ttcctcgagc
    11701 tcgatccgcg cggcgtcgac gtcaacgtgc acccggcgaa gaccgaggtg cgctttcgcg
    11761 atgcgcgcgg catgcaccag ttcgtgttcc acgcgctcaa gcgcgcgctc tcgccctcgg
    11821 cggccgaatc gccggtgagc tacgccacgg tgtccgcgcc ggggccggcg atccaggcgg
    11881 cgctggcgat gcaggagccg gtgccggagt accggccggc cgccgtcccg ctgcctgcct
    11941 cggcgcaagc gccgccgctc ggctacgccc tcgcccagct gcacggcgtg tacatcctgg
    12001 cgcagaacga ggcgggcctg gtgctggtgg acatgcacgc ggcgcacgag cgcatcctga
    12061 tggagaagct gaaactcaac ctggacgccg gcgccgtcga gcgccagagc ctgctggtgc
    12121 ccgcggtatt ccgcgccgag gcgctggatg tcgccgccgc cgaggagcac cgcgcagcgc
    12181 tggagaaact gggcctggag ctctccgtgg ccgggccgaa cgagctcgcg gtgcgcgcgg
    12241 cgcccgcgct cctcgccggc ggcgacgtgg cggcgctcgc gcgctcgctg ctcgccgagc
    12301 tgcgcgaatt cggcgccgcc caggtgctcg ccgcccggca gaacgagctg ctcggcacca
    12361 tggcctgcca tgccgcggtg cgcgccaacc gcgtgctgtc ggtgcacgag atgaacgcgc
    12421 tgctgcgcga gatggaggag accgagcgct ccggcagctg caaccacggg cgcccgacct
    12481 ggtaccagct cagcatggcg gacctggaca agctgttcct gcgtggccgc tagggcctac
    12541 gccctcctcg gccccaccgc gagcggcaag tccacgctcg ccatggcgct ggcggcggaa
    12601 ttcccgctcg agatcgtgtc gatggattcg gcgcaggtgt accgggggat ggatgtcggg
    12661 acggcgaagc cgtcgcttgc cgagcgcgca aggattcgac accacctggt ggacatcgtc
    12721 gagccgacgc ggagctatag cgcaggccgg tttcgcgcgg acgcggtccg cgcgatcggg
    12781 gaaattcaag ggcgcgggaa ggtgccgctg atcgtgggcg ggtcgatgct ctacttcaaa
    12841 gcgctgtcgc agggcatcga tgagcttccg gtggccgagc cgggattgcg tgcgcagatc
    12901 gatgcgcgcg ccgcgcgcca cggctggccg gcgctgcacg ccgacctggc gaaggtggac
    12961 ccggtgaccg cggcgcggct tgcgcccaac gacgcgcagc gcatccagcg cgcgctcgag
    13021 gtgtgggagc tgaccggcaa gccgatttcg gaattgcagc gcgcggcggg cgcgtcgctg
    13081 ccgtttgagc tcaagtcctt cgccctcgtt cccgcggacc gcggcgagct gcaccggcgc
    13141 atcgcgcagc gcttcgagcg catgctgaag gatggcctga tcgaagaact gaagggattg
    13201 cgccggcagc acgagctgca cgcggcgctg ccgagcatgc gctgcgtcgg ctaccgccag
    13261 gcctgggagt tcctcgaagg cgagtacggc aaggccgagc tgcgcgagcg gggcatcgcc
    13321 gcgacgcgcc agctcgccaa gcgccagctc acctggctgc ggagcctccc ggggctggag
    13381 cccgccgagc ggctcgccgc ggcgcttact cgggcttgat tcccacctcg cccaccaccc
    13441 ggcgccagac cgccacctgc tccctggtga acgcggcgag ttcctccggc gtcgagccgt
    13501 ggggctcgaa cgccagcttc tcgaatgcct cgcgcacctc gggccgcttg agcagcgcga
    13561 tcagctcgcg cgacaagcgc tccgtgatgt cccgcggcat gcccttcggg ccgaacaccc
    13621 cgccccaggg cgtgatcgac aggccgcgca atccggcctc cgccatggtc ggcgcctgcg
    13681 gcagcagcgg gctgcggctg ggcagcagcg tcgccagcac cttcagcctg ccctccttca
    13741 cctgcggcgc ggcggcgccc ggcgtcgaga tggcgaactg cacgcgcccg cccagcaggt
    13801 cgacgaacag cggcccgtcg cccttgtagg ggatgtgcac cacgtcgagc ttctcctgct
    13861 gcacgagctg cgccgtggag agcatggcgg tgctgtt
//
LOCUS       DASRIE010000013        15850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_788308, whole genome shotgun sequence.
ACCESSION   DASRIE010000013 DASRIE010000000
VERSION     DASRIE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788308"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFB53_00555"
     CDS             complement(<1..884)
                     /locus_tag="VFB53_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85250.1"
                     /translation="MTALLYRALKARPGELPALAWSFAYFFLMLCSYYLLRPVRDTVG
                     VALGREELQRLFLVTFVVMLVLVPVFGWLCARLPRARLLPLVYAFFALNLVAFSAHLP
                     PAVFFVWLSVFNLFVVSVFWSFMSDLFQPGQAARLYGTIAAGGSCGAIAGPLIAAQSP
                     LGASGLLVLAAVLLAAAVICIVMLSRWARSHPRAGDPPAEQPMGGSILAGVRAAFSSP
                     FLAAICGYLFCYTALSTALYFQQVSIVREALPDPAEQTRFFARVDLTVNSLTLLIQLF
                     AFSRLAQAIGPTGLLVLMP"
     gene            903..1499
                     /locus_tag="VFB53_00560"
     CDS             903..1499
                     /locus_tag="VFB53_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN85251.1"
                     /translation="MGTGGWIALGIVVVVVIWAIMIYNGLVALRNRYKNAFSQIDVQL
                     KRRYDLIPNLVETVKGYMAHERGTVEAVIKARGDAVGVAQRAAAAPGDPAAMRGLSQA
                     EGVLGGALGRLLAVFEQYPDLKANQNVAALQEELTSTENKVAFARQAYNDSVMEYNTK
                     RESFPDTVFAGMFGFQAAELLQATESAEERKAPKVSFS"
     gene            1506..3311
                     /locus_tag="VFB53_00565"
     CDS             1506..3311
                     /locus_tag="VFB53_00565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN85252.1"
                     /translation="MGFFAQQDRARRATARLAALYVLALAAIVVCANLLVVPLYTFFF
                     SQAASAALYATVSLVTLVVIALGSVEIMARVSLGEGDLALALAGRRIGRSSGIDAERR
                     LVNVVDEMAIASGLAAPPLYVLSREEGINAFAAGRSPNQAIIVVTKGALEHLSRDELQ
                     AMVAHEFSHILNGDIRLNLRAACALQGIVFLSAIGRFMMQYYSGYGTEERRRFLHPPF
                     ALLGAGFFVLGSAGLVAARLIQSAIAREREFLADACAVQYTRNADALCGALARIQSSP
                     AGSRLLNWHADALAHMLFAPHTLRSWFSSHPPIEARMRRANPHLAPRFYFERKNLAEK
                     PKPAEKPKTQPRAPARLTAVSALIASIGEPSSATLEYASGVLAYLPGSIREALGNAAG
                     AEAVMLGCVIDAEATARSRQLRALAALGREELARKAEVLAPLIAQLDRAYRLPILALA
                     LPVLRTELDEAGRGAFLAAMRAAIQADARMTLGEFVLDTILDWNLGPRSKRAGGVRFQ
                     SRGQLAAECALILALLGHEKRNFQLAEVSQALERLSQLAPLEKELFLASCAEVVAADG
                     NVRLVEHELLRAVAAALDCPMPPTIAALDPRLLRK"
     gene            3308..5194
                     /locus_tag="VFB53_00570"
     CDS             3308..5194
                     /locus_tag="VFB53_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZN85253.1"
                     /translation="MTTFFEHQGLARRNSRLMVVLFALAVLLTVIAVDVALGVIYAMS
                     AGEPAPVSVYVLGALATAGLILCVSLFNIARLSEGGVAVARMVGARRVAPDTLDPLER
                     RLRNVVEEMAIASGVRVPEVYVMDGERGINAFAAGWDPSAAVVAVTRGTLQALTRDEL
                     QAVVAHEFSHILNGDMRLNVRMLGVLAGIVFLASVGEFLMRSMRGSRDAKGLALGGLA
                     LFVIGYVGLFFARLIKSAVSRQREFLADASSVQFTRNPDGIAGALDQIRSSGAGTLIA
                     NRYAEEMSHMFFGAAVKVRLAALFDTHPALDERIRRVHPQFQPGEYRAKRVAALPLEA
                     PEPRPLAQKGRRAGDLSAAWGRTPEEALALVGTLDPAKVDFAARLLRMLPAPLHASLE
                     SAEGAAALMVALMLAEPEDALKQQLDALGASPLAARARELAPLARNLGPALRLPVVDL
                     ALPALKAAPAAAKQELLAALGAAINADRRVSLHEFAVLALVREQLEPRPKPAQTKQIA
                     ELKNEAALVLALLAHAGTRLDATGKRGAELQAALETGATLLGIPAALAPAEFSLESVT
                     AALEALRRLAPMQQAVLVKGLFAAVTLDGTIRVAEAELMRLVGAVLGCPLPPLFDSLL
                     GEPL"
     gene            complement(5162..6055)
                     /locus_tag="VFB53_00575"
     CDS             complement(5162..6055)
                     /locus_tag="VFB53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HZN85254.1"
                     /translation="MPQTKELAQGGLPRGLPENVELTEVPFFPQLEYQCGPAALATVL
                     ANAGVKTTPDELVPQVYLPARKGSLQVEMLAAARRHGMVSYPLAPRFEDLLREIAAGN
                     PVIVLQNLGVFTAGWHYAVAVGYDYSLGTLVLRSGTQERDVMPFAAHEVFWMRSGYWA
                     MVALPPDRIPATAEEKSWLNAIAAFERIGDANGARVAYKTFLGRWPENVNAHIGLANT
                     HYTLGELAQAEAVLRDAARREPESVVVLNNLAQTLSDLGRNEEALPLTDKALAMGGPF
                     SGAVQKTRETILQRLAKKRVE"
     gene            complement(6139..6528)
                     /locus_tag="VFB53_00580"
     CDS             complement(6139..6528)
                     /locus_tag="VFB53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HZN85255.1"
                     /translation="MYPTHPPRTFMKSVWARTLCRLLMALMVWAPMQFAQAGMIGTEQ
                     VASAASQADRNTVLQFLGRAEVTSQLQSLGVDASTVKDRVAAMSDQEVQSLAGKIQSM
                     PAGADTTGTVLLLLVVAAVVWWVWFRR"
     gene            6626..9409
                     /locus_tag="VFB53_00585"
     CDS             6626..9409
                     /locus_tag="VFB53_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZN85256.1"
                     /translation="MVEFQRLEAELRAALRGEVRFDKGMRAAYAADASNYRQVPIGVV
                     LPRSVDDIVAALRVCSAHGVPVLPRGGATSLNGQCVNVAVVVDCSKYLDRVLAVDPHA
                     RLARVEPGVVCDALRDAAEAHALTFAPDPATHSRCTLGGMIGNNSCGPHSVMAGTTVD
                     NIERLEVLTYDGARFWCGPTPDSDLQEILSGRDRKAEIYRNLKAIADKYADHIRAGFP
                     KIKRRVSGYNLDQLLPENGFNVARALVGSEGTCALTLQAEARLVKSPQKRVLVVLGFP
                     DIGAAGDAVPRVLAAGPIACEGLDEAIIGGLRERRLRLDDIALLPPGKAWLMVEFGAD
                     TQEEAVARASSLGRNLITDKNMMARLWTIRETGASATSLNLGGKGADPVVGWEDAAVD
                     PMRLGGYLREFQALVDRYGYRTSLYGHFGDGCIHARINFELRTPAGLAHWRRFLTEAA
                     HLVVKYGGSLSGEHGDGQAKGELLPIMFTPELMQAFREFKRAWDPQNRMNPGKLIDAR
                     PFDANLRLGPDYKPVTVKTHFPFLTPVGDGFVRATEHCIGMGKCRASTGGTMCPSYRA
                     TREERYSTRGRGRLLSEMLRGEVITDGWESEEVKEALDWCLACKGCRSDCPTHTDMAA
                     YKAEFLSHYYEKRSRPRQAWSMGRIGEWAPLASRFSLISNLASSLKTVKALAGVAPER
                     ALPKFAARSFRSQFKPGGKGEPVVLFDDTFNNHFRPQTAAAAQRVLEAAGCAVELPAR
                     RVCCGRPYYDYGMLQEAKRSLERVLEVLPLDRPIVVLEPGCLSVFRDELRQLFPDDAR
                     AARLAAQALSLPELLNRRNFKLDVGAQVFMHAHCHQKSLWGTSADASLLKNSGIDLIA
                     PDTGCCGMAGSFGYKPQHAEASARIAGLALLPALEAAKDAVVVANGFSCREQIEALSG
                     RRTLHIAELLAAR"
     gene            9417..10643
                     /locus_tag="VFB53_00590"
     CDS             9417..10643
                     /locus_tag="VFB53_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN85257.1"
                     /translation="MSVEVLLYVALVCLVSGFAHGAIGFGFPLVATPLVALALDMKTA
                     ITLLAPVTLVLVLISGFTGGGVRELLRRFWFIPVAMAIGAFLGTRLLLAAPPEPFTLV
                     LALMLLFYLNLDRVGRGKSEAVMRLRVPVGFAFAFAAGIFEAIANVAGPMLLVYFMLL
                     GAAPSQIVQTLNICFSVGKGSQVATLAASGALTPATWLTVAGLSVPAVAALALGMRLR
                     SRIDAQTYRAWLRKALWVMAIVLIAQFSASVLASEPLFRAIDRHEEAAALELVARGQA
                     DLEARNPEGDTALHRAIETGMRRLLEALLARGADLRARTKNGETALHLAALHPEPAFA
                     DLLLAAGADPRALNADGESPLHWAALSGHIVVAQRLLARGADARQRTAKGQSARDYAR
                     REGHAQIARLLERFDQ"
     gene            complement(10620..11597)
                     /locus_tag="VFB53_00595"
     CDS             complement(10620..11597)
                     /locus_tag="VFB53_00595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN85258.1"
                     /translation="MKLSILDQSPVIAGLGARRAIEETIKLARRADELGFHRYWLAEH
                     HAIAALADPCPEVLLARLGAETRRLRIGTGGVLLPYYSAFRTAESFRMLEALYPGRID
                     LGIGRAPGGDARTAHAVGGGVFPDASRFPQQVAELNDLLKGKARPVRLQPEVDTAPEV
                     WLLGSSDYSGALAAQLGLPFAFAHFINPRGGDEVTRGYKANFNAAANSGAAPRAIVCT
                     FAILAESDAEAERLAAPIDLRRLHMALNIDSPVPTYEEAARHRYSDEERRYVLSQRAR
                     AVIGGPEKARLELRNLMQKYHADELMVLTITGDYDSRRRSYELLIEAFE"
     gene            11801..12130
                     /locus_tag="VFB53_00600"
     CDS             11801..12130
                     /locus_tag="VFB53_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85259.1"
                     /translation="MTTIRLKQELYSFAHTFWSCVLLSLVLGGIGGTIYKVISPEGWI
                     AQAFGRSLSAGAAALGSMALVGALAWFSRTWGDPTHRTLLWHFLIWSFAAAGFLYLAQ
                     LWTKGTL"
     gene            complement(12098..13774)
                     /locus_tag="VFB53_00605"
     CDS             complement(12098..13774)
                     /locus_tag="VFB53_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85260.1"
                     /translation="MRDEAVLILAPTAKDAALCRKILEEAGVECALCADVAELCEGID
                     RGAGIAMLGEEALTAESLQMLRVAIRRQPAWSDFPLLILTEEGADSEVVLRTLETLGN
                     VTLLERPVRVPALVSSVRAALRARRRQYQIRDHLSENNRVAAALLEADRRKDEFLAIL
                     AHELRNPLAPLRNALEAMRLKPHDREAASWARALMERQVAQMVRLIDDLLDLSRVSRG
                     RIELKHERAELATLVQGALDVCGPAVAQSRHRLAVELPQEPVTLTCDPTRLVQVICNL
                     LSNAVKYTPPGGRIGIGARRVDGQVEISVRDSGLGIPPDMLSRVFEMFTQVPHSPERS
                     QGGLGIGLTLVKRLVELHGGTVLARSDGPGKGSEFVVRLPQHAAEPAAAAAAAPAPPA
                     GGARHRILVADDNRDAADSLAVMLRIAGHEVRTAYDGQEALEIAEAFRPALALLDLGM
                     PRLNGYEAARRLRASELGREMQLIALTGWGQPDDRSRSLAAGFDYHVVKPVDPSLLER
                     LLASEKKTGPACRGEDGPRGGVDLSDRPLEVNALREDRVGLEGPLRPELR"
     gene            complement(13782..15272)
                     /locus_tag="VFB53_00610"
     CDS             complement(13782..15272)
                     /locus_tag="VFB53_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZN85261.1"
                     /translation="MQANTDAPAVATGIAGLDNILGGGFTPNRIYLVEGDPGSGKTTL
                     ALQYLLEGRRAGESGMYVSLSETKEELDGVARSHGWSLEGIHCHELLPSDESLLPDAQ
                     SRLFHPSEVELSDTTRAILAEVERRKPSRVVFDSLSEMRLLAQHPLRYRRQVLALKQF
                     FIGRACTVLLLDDRTATDNDLQLHSLAHGVISLSHMAQEYGGERRRLRVMKMRGRAFR
                     GGYHDFMIRTGGLEVFPRLVSAEHRRSFDAEPLKSGVESLDALTGGGLHRGTSALLMG
                     PAGAGKSTLATQYAVAAAERGERAVLYIFDESVGTLLMRSQALGIGLKRHVEAGRIRV
                     QQVDPGELSPGEFIYGIRRAVEEEAARVVVIDSLNGYLNAMPEERFLTIQLHEMLTYL
                     GQQGVVTLMVVAQKGLLGSGMQTPVDASYLADTVIALRYFEAKGKLRRAISVVKKRSG
                     AHEDTLRELSMRRGGVALGPPLAEFHGIMSGIPGFAGKDEADLARR"
     gene            complement(15385..>15850)
                     /locus_tag="VFB53_00615"
     CDS             complement(15385..>15850)
                     /locus_tag="VFB53_00615"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HZN85262.1"
                     /translation="AVLGLIGGTSLGIFVYPYVFLADIVATEKVQPAPAGQPERKVLA
                     RGSFVHANPSDPIHYGKGKVTVYDGLLYLEPDFEVGPGPKYHVYLVPEKEVMPSTNVA
                     RTMFVDLGRLKAFKGSQNYDVPVGVDVGKFGSVVVWCEQFGVLISPAKLTPP"
BASE COUNT         2279 a         5548 c         5629 g         2394 t
ORIGIN      
        1 ggcatgagta ccagcagccc ggtcggcccg atggcctgcg ccaggcgcga gaaggcgaag
       61 agctggatca gcagggtcag gctgttcacg gtgaggtcga cgcgcgcgaa gaagcgcgtc
      121 tgctcggcgg ggtcgggcag cgcctcgcgc acgatgctca cctgctggaa gtagagcgcg
      181 gtcgagagcg cggtgtagca gaagaggtag ccgcagatcg ccgcgaggaa gggcgaggag
      241 aatgccgcgc gcaccccggc gaggatcgag ccgcccatcg gctgctccgc gggtggatcg
      301 ccggcccggg gatggcttcg cgcccagcgc gacagcatca cgatgcagat cacggccgcc
      361 gcgaggagca ccgcggcgag caccagcagg ccgctcgcgc cgagcggcga ctgcgccgcg
      421 atcagcggcc cggcgatggc gccgcagctc ccgccggcgg cgatcgtgcc gtagagccgc
      481 gccgcctgcc cgggctggaa gaggtcggac atgaagctcc agaacaccga caccacgaag
      541 aggttgaaca ccgaaagcca gacgaagaac accgccggcg gcaggtgggc cgagaaggcc
      601 acgaggttga gcgcgaagaa ggcatagacg agcggcagca ggcgcgcgcg cggcagcctg
      661 gcgcacagcc agccgaacac cggcacgagc accagcatga cgacgaaggt caccaggaaa
      721 agccgctgca gctcctcccg cccgagcgcc acgccgaccg tatcgcgcac cgggcgcagc
      781 aggtagtagc tgcacagcat caggaaaaag taggcgaacg accaggcgag cgccggcagc
      841 tccccgggcc gcgccttcag cgcgcggtat agtagggccg tcacgcgacc aaggggggat
      901 gcatgggcac aggaggctgg atcgcgctgg gcatcgtagt cgtggtcgtc atctgggcga
      961 tcatgatcta caacggcctg gtggcgctgc gcaaccggta caagaacgcc ttttcgcaga
     1021 tcgacgtgca gctcaagcgg cgctacgacc tgattcccaa cctggtcgag accgtcaagg
     1081 gctacatggc gcacgagcgc ggcaccgtcg aggcggtgat caaggcgcgc ggcgacgccg
     1141 tgggcgtggc gcagcgcgcg gccgcggcgc ccggcgaccc cgccgcgatg cgcggcctgt
     1201 cccaggccga gggcgtgctc ggcggcgcgc tcggccgcct gctcgcggtg ttcgagcagt
     1261 acccggacct gaaggcgaac cagaacgtcg cggcgctgca ggaggagctc accagcaccg
     1321 agaacaaggt cgccttcgcg cgccaggcct acaacgactc ggtgatggaa tacaacacca
     1381 agcgcgagtc cttccccgac accgtgttcg cggggatgtt cggcttccag gcggcggagc
     1441 tgctgcaggc gaccgagtcg gccgaggagc gcaaggcgcc gaaggtttcg ttctcctagc
     1501 cgctcttggg gttcttcgcg cagcaggacc gcgcccgccg cgcgaccgcg cggctcgccg
     1561 cgctttacgt gctcgcgctc gccgcgatcg tggtctgcgc gaacctgctc gtggtgccgc
     1621 tttacacgtt tttcttctcg caggcggcgt ccgcggccct ttacgccacg gtgagcctcg
     1681 tcacgctggt ggtgatcgcg ctcggcagcg tcgagatcat ggcgcgggtc tcgctcggcg
     1741 agggcgacct cgcgctcgcc cttgccggac gccgcatcgg gcgctcgagc ggcatcgatg
     1801 ccgagcggcg cctggtcaac gtggtggacg agatggcgat cgcctcgggc ctcgccgcgc
     1861 cgccgctgta cgtgctgagc cgcgaggagg gcatcaacgc cttcgccgcc gggcgctcgc
     1921 ccaaccaggc gatcatcgtg gtgacgaagg gagcgctcga gcacctctcg cgcgacgagc
     1981 tgcaggcgat ggtggcgcac gagttcagcc acatcctgaa cggcgacatc cggctcaacc
     2041 tgcgcgcggc gtgcgcgctg cagggcatcg tgttcctctc cgccatcggc cgcttcatga
     2101 tgcagtacta ctccggctac ggcaccgagg agcgccgccg cttcctgcac cctcctttcg
     2161 cgctgctcgg cgcgggcttc ttcgtgctgg gctcggccgg cctggtcgcg gcgcggctga
     2221 tccagtccgc catcgcgcgc gagcgcgagt tcctggccga cgcctgcgcg gtgcagtaca
     2281 cgcgcaacgc cgacgcgctc tgcggtgcgc tcgcccgcat ccagtcgagc ccggcgggct
     2341 cgcgcttgct caactggcat gcggatgcgc tcgcgcacat gctgttcgcg ccgcacacgc
     2401 tgcgctcgtg gttttcctcg catccgccga tcgaggcccg catgcggcgc gccaatccgc
     2461 acctggcgcc gcgtttctat ttcgaacgga agaaccttgc cgaaaagccc aagccggccg
     2521 agaaacccaa gacccagccc cgggctccag cgcggctcac ggcggtcagc gcgctgatcg
     2581 cctccatcgg cgagccgagc agcgcgacgc tcgagtacgc atccggggtg ctcgcctatc
     2641 tgccgggctc gatccgcgag gcgctcggca acgccgcggg cgccgaggcg gtgatgctcg
     2701 gctgcgtcat cgacgccgag gccacggcgc gcagccgcca gctgcgcgcc ctggccgcgc
     2761 tcggccggga ggagctcgcg cgcaaggccg aggtgctggc gccgctcatc gcccagctcg
     2821 atcgcgccta ccggctgccg atcctcgcgc tcgcgctgcc ggtgctgcgc accgagctcg
     2881 acgaggccgg gcgcggcgcg ttcctcgccg cgatgcgggc cgcgatccag gccgacgcga
     2941 ggatgacgct cggcgagttc gtgctcgaca cgatcctcga ctggaacctc ggaccgaggt
     3001 cgaagcgcgc gggcggcgtg cgcttccaga gccgcggcca gctcgccgcc gagtgcgcgc
     3061 tgatcctcgc cctcctggga cacgagaagc gcaacttcca gctcgcggag gtttcgcagg
     3121 cgctcgagcg cctgtcccag ctcgccccgc tcgagaagga gctgttcctc gcctcttgcg
     3181 ccgaagtggt cgccgccgac ggcaacgtcc ggctggtcga gcacgagctc ctgcgcgcgg
     3241 tcgcggctgc gctcgactgc ccgatgccgc ccaccatcgc cgcgctcgat ccgcggctgc
     3301 tgcgcaagtg acgaccttct tcgagcacca ggggctcgcg cgccgcaata gccggctgat
     3361 ggtggtgctg ttcgcgctgg cggtgctcct gacggtgatc gcggtcgacg tggctctggg
     3421 cgtgatctac gccatgagcg ccggggagcc ggcgccggtg tcggtgtacg tcctgggcgc
     3481 gctcgcgacc gccgggctga tcctctgcgt gagccttttc aacatcgcgc ggctctccga
     3541 gggcggcgtc gccgtggcgc gcatggtggg cgcgcgccgc gtcgcgccgg acaccctcga
     3601 tccgctggag cggcggctgc gcaacgtggt cgaggagatg gcgatcgcct cgggcgtccg
     3661 cgtgccggag gtctacgtga tggacggcga gcgcggcatc aacgccttcg ccgccggctg
     3721 ggatccgtcc gccgccgtgg tggcggtcac gcgcggcacg ctgcaggcgc tgacgcgcga
     3781 cgagctgcag gcggtggtgg cgcacgagtt cagccacatc ctgaacggcg acatgcggct
     3841 caacgtgcgc atgctcggcg tgctcgccgg gatcgtgttc ctcgcctcgg tgggcgagtt
     3901 cctgatgcgc agcatgcgcg gctcgaggga cgcgaagggc ctcgcgctcg gcggcctcgc
     3961 gctcttcgtg atcggctacg tcggcctgtt cttcgcgcgc ctcatcaagt cggcggtgtc
     4021 gcgccagcgc gagttcctcg ccgacgcttc cagcgtgcag ttcacgcgca atcccgacgg
     4081 catcgccggc gcgctcgacc agatccgctc ctcgggcgcg ggcacgctga tcgccaaccg
     4141 ctacgccgag gagatgtcgc acatgttctt cggcgcggcg gtgaaggtgc ggctcgccgc
     4201 gctgttcgac acccacccgg cgctcgacga gcgcatccgc cgggtgcatc cgcagttcca
     4261 gcccggggag taccgggcga agcgcgtggc cgcgctgccg ctcgaggcgc cggaaccgcg
     4321 gccgctcgcg cagaagggaa ggcgcgcggg cgacctgtcg gccgcctggg gacgcacgcc
     4381 cgaggaggcg ctcgcgctcg tcggcacgct cgacccggcg aaggtggatt tcgccgctcg
     4441 gctgctgcgc atgctgccgg cgccgctgca tgcgagcctc gagagcgccg aaggcgcggc
     4501 ggccttgatg gtcgcgctga tgctcgccga gcccgaggac gcgctcaagc agcagctcga
     4561 cgcgctcggc gcaagtccgc tcgccgcgcg tgcgcgcgag ctcgccccgc tcgcgcgcaa
     4621 cctcggcccg gcgctgcgcc tgccggtggt cgacctggcg ctgccggcgc tcaaggccgc
     4681 gcccgccgcg gcgaagcagg agctgctcgc cgcgctcgga gcggcgatca acgccgaccg
     4741 gcgggtctcg ctgcacgagt tcgcggtgct tgcgctggtg cgcgagcagc tcgagccgcg
     4801 gccgaagccc gcgcagacga agcagatcgc ggagctcaag aacgaagcgg cgctggtgct
     4861 cgcgctgctg gcgcatgccg gcacgcgcct ggacgcgacc ggcaagcgcg gcgcggagct
     4921 gcaggcggcg ctcgagaccg gcgcgacgct cctcggcatc ccggccgcgc tcgcgccggc
     4981 ggaattctcc ctggagagcg tcaccgccgc gctggaagcg ctgcgccgcc tcgcgccgat
     5041 gcagcaggcg gtgctggtga agggattgtt cgccgcggtg acgctcgacg gcacgatccg
     5101 cgtcgccgag gcggagctga tgcggctggt gggcgcggtg ctgggctgcc cgctgccgcc
     5161 gctattcgac tcgcttcttg gcgagcctct gtaggatcgt ctcgcgggtc ttctgcaccg
     5221 cgccggagaa cggcccgccc atggcgaggg ccttgtcggt gagcggcagc gcttcttcgt
     5281 ttcggccgag gtccgagagc gtctgcgcga ggttgttcag caccaccacc gattcgggct
     5341 cgcgccgcgc ggcgtcgcgc agcacggcct ccgcctgcgc cagctcgccc agggtgtagt
     5401 gcgtgttggc aaggccgatg tgcgcgttca cgttctccgg ccagcggccg aggaacgtct
     5461 tgtaggcgac gcgggcgccg ttggcgtcgc cgatgcgctc gaatgcggcg atcgcgttga
     5521 gccagctctt ctcttccgcg gtcgcgggga tccggtcggg cggcagcgcg accatcgccc
     5581 agtagccgct gcgcatccag aacacctcgt gcgcggcaaa cggcatcacg tcgcgctcct
     5641 gcgtgcccga gcgcagcacc agcgtaccga gcgaatagtc gtagccgacc gcgacggcgt
     5701 aatgccagcc ggcggtaaag acgccgaggt tctgcagcac gatcaccggg ttccccgccg
     5761 cgatctcgcg cagcaggtcc tcgaagcgcg gcgcgagcgg gtaggacacc atgccgtggc
     5821 gccgcgccgc ggcgagcatc tcgacctgga ggctgccctt gcgcgccggg aggtagacct
     5881 gcggcacgag ctcgtccggg gtggtcttga cgccggcgtt ggcgaggacc gtcgcgagcg
     5941 ccgccgggcc gcactggtac tcgagctgcg ggaagaacgg cacctcggtg agctccacgt
     6001 tctccggcag cccccgcggc aggccgccct gcgccagctc cttcgtctgc ggcatccagc
     6061 tcgcgcagcc ggcggcgagc gccaaaatga aaacgcccgc caataggcgg gcgcttcgtt
     6121 gcacggctgg gccgatggct agcggcggaa ccaaacccac cacaccacgg cggcgaccac
     6181 gaggagcagc agcacggtgc cggtcgtgtc ggcgccggcg ggcatgctct ggatcttgcc
     6241 ggcgagcgac tgcacttcct ggtccgacat cgcggctacg cggtccttga ccgtggaggc
     6301 gtcgacgccg agcgactgca gctggctcgt cacctcggcg cggccgagga attgcagtac
     6361 ggtgttgcgg tcggcttgcg aagccgcgga agccacttgc tcggtgccga tcattcccgc
     6421 ctgcgcgaac tgcatcggcg cccaaaccat cagcgccatc aacaagcggc agagcgttct
     6481 tgcccaaact gatttcataa aggtcctcgg ggggtgagtg ggatacatct tgacagtgac
     6541 gacccgcaac cggttccgat cgtctgtgaa ccagcctagt cgccaccccc tggcaagtca
     6601 agcacgcatg caagaatcgc gacaaatggt cgaatttcaa cgactcgaag cggagctgcg
     6661 cgccgcgctg cgcggcgagg tccgcttcga caagggcatg cgggccgcct acgcggccga
     6721 tgcgtcgaac taccgccagg tgccgatcgg cgtcgtgctg ccccgcagcg tagacgacat
     6781 cgtcgcggcg ctgcgcgtgt gctcagcgca cggcgtgccg gtgctgccgc gcggcggcgc
     6841 gacctcgctc aacggccagt gcgtcaacgt agcggtggtg gtcgactgct cgaagtacct
     6901 cgatcgagtg ctcgcggtgg atccgcacgc gcgcctcgcg cgcgtcgagc ccggcgtggt
     6961 atgcgacgcg ctgcgcgacg ccgccgaggc gcacgcgctc accttcgcgc ccgacccggc
     7021 gacgcattcg cgctgcacgc tcggcggcat gatcggcaac aactcgtgcg gtccgcactc
     7081 ggtgatggcc ggcaccacgg tcgacaacat cgagcgcctc gaagtcctga cctacgacgg
     7141 cgcgcgcttc tggtgcggcc cgacgcctga ttcggatctg caggaaatcc tttccgggcg
     7201 ggatcgaaag gcggaaatct ataggaacct gaaagccatc gccgacaagt acgccgacca
     7261 catccgcgcc ggctttccga agatcaagcg gcgcgtctcg ggctacaacc tggaccagct
     7321 gctgcccgag aacggcttca acgtggcgcg cgcgctggtc ggctccgagg gcacctgcgc
     7381 gctcacgctg caggcagagg cgcggctcgt gaagagcccg cagaagcgcg tgctcgtggt
     7441 cctcggcttc cccgatatcg gcgcggcggg cgacgcggtg ccgcgggtgc tcgccgccgg
     7501 gccgatcgcg tgcgaggggc tcgacgaggc gatcatcggc ggtctgcgcg agcgccggct
     7561 gcgcctggac gacatcgcgc tgctgccgcc gggcaaggcg tggctgatgg tcgaattcgg
     7621 cgccgacacg caggaggagg cggtggcccg cgcatcctcc ctcgggagga atctgatcac
     7681 cgacaagaac atgatggcgc ggctctggac gatccgcgag accggcgcct ccgcgacttc
     7741 gctcaacctc ggcggcaagg gcgccgatcc ggtcgtcggc tgggaggacg cggcggtcga
     7801 cccgatgcgc ctgggcggct acctgcgcga gttccaggcg ctggtcgatc gttatggcta
     7861 ccgcacctcg ctctacggcc acttcggcga cggctgcatc cacgcgcgca tcaacttcga
     7921 gctgcgcacg cccgcgggcc tcgcgcactg gcggcgcttc ctcaccgagg ccgcgcacct
     7981 cgtggtgaag tacggcggct cgctgtcggg tgagcacggc gacggccagg cgaagggcga
     8041 gctgctgccg atcatgttca cgcccgagct gatgcaggcg ttccgcgagt tcaagcgcgc
     8101 gtgggatccg cagaaccgca tgaacccggg caagctcatc gacgcccggc cgttcgacgc
     8161 caatttacgg ctcgggccgg actacaagcc ggtaactgta aagacgcatt tcccgtttct
     8221 cacgccggtt ggcgatggct ttgtccgcgc cacggagcac tgcatcggca tgggcaagtg
     8281 ccgcgcgagc acgggcggca cgatgtgccc gagctaccgg gcgacgcgcg aggagcgcta
     8341 ctccacgcgc ggccgcggcc ggctgctctc cgagatgctg cgcggcgagg tgatcaccga
     8401 cggctgggag agcgaggagg tgaaggaggc gctcgactgg tgcctcgcct gcaagggctg
     8461 ccgcagcgac tgcccgacgc acaccgacat ggccgcgtac aaggcggagt tcctgtcgca
     8521 ctactacgag aagcgctcgc ggccgcggca ggcctggtcg atgggccgca tcggcgagtg
     8581 ggcaccgctg gcatcgcgct tttcgcttat ttccaacctt gcttcctcct tgaagaccgt
     8641 gaaggcgctg gcaggcgttg ctccggaaag ggcgctgccc aagttcgccg cgcgcagctt
     8701 ccgctcgcag ttcaagcccg gcggcaaagg cgagccggtg gtgctgttcg atgacacctt
     8761 caacaaccat ttccgtccgc agactgcggc ggccgcgcag cgcgtgctcg aagccgccgg
     8821 atgcgcggtg gagctgccgg cgcggcgcgt gtgctgcggc cggccctact acgactacgg
     8881 catgctgcaa gaggccaaac gctcgctcga gcgcgtgctc gaggtcctgc cgctcgaccg
     8941 gcccatcgtc gtgctggagc ccggctgcct ctcggtgttc cgcgacgagc tgcggcagct
     9001 cttcccggac gacgcgcgcg cggcgcgcct ggcggctcaa gcgctctccc tgcccgagct
     9061 ccttaaccgg cgtaatttca agcttgacgt cggggcgcag gttttcatgc acgcccattg
     9121 ccatcagaag tcgctctggg gcacgagcgc ggatgcatcg cttctcaaga actccggaat
     9181 cgatctcatc gcgcctgaca ccggctgctg cggaatggcg ggctcgttcg gctacaagcc
     9241 gcagcatgcc gaggcgtcgg cgcgcatagc ggggctcgcg ctgctgcccg cgctcgaggc
     9301 ggcgaaagac gcggtggtgg tggcgaacgg cttttcctgc cgcgagcaga tcgaggctct
     9361 ctcgggccgg cgcacgctgc acatcgcgga gctcctcgcc gcccgatgaa tacctggtga
     9421 gcgtcgaggt gctcctgtac gtggcgctgg tatgcctggt ctccggcttc gcgcacggcg
     9481 cgatcggatt cggctttccg ctggtcgcga cgccgctcgt cgcgctcgcg ctcgacatga
     9541 agaccgcgat cacgctgctc gcgccggtga cgctggtgct ggtgctcatt tccggcttca
     9601 ccggcggcgg cgtgcgcgag ctgctgcggc gcttctggtt cattcctgtg gcgatggcga
     9661 tcggcgcctt cctcggcacg cggctgctgc tggccgcgcc gcccgagccc ttcacgctgg
     9721 tgctcgcgct gatgctgctt ttctacctga acctcgaccg cgtcggccgc ggcaagagcg
     9781 aggcggtgat gcggctgcgc gtgccggtcg gcttcgcctt cgcgttcgcc gccggcatct
     9841 tcgaggcgat cgccaatgtc gccgggccga tgctgctcgt gtacttcatg ctgctcggcg
     9901 cggcgccttc gcagatcgtg cagaccctga acatctgctt ttccgtcggc aagggctcgc
     9961 aggtggcgac gctcgcggcc tccggcgcgc tgacgccggc cacctggctc acggtggcgg
    10021 gcctgagcgt gccggcggtg gcggcgctgg cgctgggcat gcgcctgcgc agccgcatcg
    10081 acgcccagac ctaccgcgcc tggctgcgca aggcgctctg ggtgatggcg atcgtgctga
    10141 tcgcgcagtt ctcggcgagc gtgctcgctt ccgagccgct gttccgcgcg atcgaccggc
    10201 acgaggaggc ggcggcgctc gagctggtgg cgcgcggcca ggccgacctg gaggcgcgca
    10261 acccggaagg cgacaccgcg ctgcaccgcg cgatcgagac cgggatgcgc cggctgctcg
    10321 aggcgctgct cgcgcgcggc gccgacctgc gggcgcgcac gaaaaacggc gagaccgcgc
    10381 tccacctggc cgcgctgcat cccgagccgg cgttcgccga cctgctgctc gccgcgggcg
    10441 cggatccgcg cgccctcaat gccgacggcg agtcgccgct gcactgggcg gcgctcagcg
    10501 ggcacatcgt agtggcgcag cgcctgctcg cgcgcggcgc cgacgcgcgg cagaggaccg
    10561 cgaaagggca gagcgcgcgc gactacgcgc gccgcgaggg ccacgcgcag atcgcaagac
    10621 tactcgaacg cttcgatcag tagctcgtag gagcgccggc gcgagtcgta gtcgccggtg
    10681 atggtcagca ccatcagctc gtccgcatgg tatttctgca tgagatttct caattcaagc
    10741 cgggccttct cgggccctcc gatcaccgcg cgggcgcgct gcgagagcac gtagcggcgc
    10801 tcctcatcgc tgtagcggtg gcgcgccgct tcctcgtagg tcggcaccgg cgagtcgatg
    10861 ttgagcgcca tgtgcaggcg ccgcaggtcg atcggcgccg ccaggcgctc ggcctcggcg
    10921 tcgctttcgg cgaggatcgc gaaggtgcac acgatcgccc gcggcgccgc gccggaattc
    10981 gccgccgcgt tgaaattcgc cttatagccg cgcgtgacct cgtcgccgcc gcgcgggttg
    11041 atgaagtgcg cgaaggcgaa gggcaggccg agctgcgcgg cgagcgcgcc gctgtagtcg
    11101 gaggagccga gcagccagac ttccggcgcg gtgtcgacct cgggctgcag gcgcaccggc
    11161 cgcgccttgc ctttcaacag gtcgttcagc tcggcaacct gctgcggaaa gcgcgacgcg
    11221 tcggggaaca cgccgccgcc caccgcgtgc gcggtgcgcg cgtcgccgcc gggcgcgcgc
    11281 ccgatgccga ggtcgatgcg ccccggatag agcgcctcga gcatgcggaa gctctcggcg
    11341 gtgcggaatg cgctgtagta gggcagcagg accccgccgg tgccgatgcg caggcgccgc
    11401 gtttccgcgc cgagccgcgc gagcagcacc tctggacatg gatcggcgag cgccgcgatc
    11461 gcatggtgct cggcgagcca gtagcggtgg aagccgagct cgtcggcgcg ccgagcgagc
    11521 ttgatcgttt cctcgatcgc gcggcgcgcg cccaagccgg cgatcaccgg ggactggtcg
    11581 aggatggaga gcttcatcga attgtcgttt cgacccgaca gctaaaaaca gcctgcgttc
    11641 aaggggggat ggcgtagtgt gaaatgtgtc gtcgtgtcgg cacgacaaag aatcgttcgg
    11701 aactgtctca cgaatgcgcc ataaggcact gtttccaaaa cacgacgcgt tcctggcacg
    11761 cgctttgcaa aagagtgtcc gagacgaggg gagaggacac atgaccacca ttcggctaaa
    11821 gcaggaactc tatagctttg cccacacctt ctggagctgc gtgctcctca gcctggtgct
    11881 gggcggcatc ggcggcacga tctacaaggt gatttctccc gaaggctgga tcgcgcaggc
    11941 cttcggtcgc agcctttccg cgggcgcggc tgcgctcggc tcgatggcgc tggtcggcgc
    12001 gctcgcctgg ttctcgcgca cctggggcga tcccacccac cgcacgctgc tgtggcactt
    12061 cctgatctgg agcttcgccg ccgccggatt cctgtaccta gcgcagctct ggacgaaggg
    12121 gaccctctaa gccgacccgg tcctcccgca aagcgtttac ctcaagcggt cgatccgaaa
    12181 gatcgactcc tccccggggc ccgtcctccc ccctacaggc gggccccgtt tttttttcgc
    12241 tggcgagcaa ccgctcgagc agggaagggt ccaccggctt caccacgtgg tagtcgaagc
    12301 cggcggcgag cgagcggctg cggtcgtcgg gctggcccca gccggtgagc gcgatgagct
    12361 gcatctcgcg cccgagctcg ctcgcgcgca ggcgccgcgc cgcctcgtac ccgttgagcc
    12421 gcggcatgcc gaggtcgagc agcgccagcg ccgggcgaaa ggcttcggcg atctcgagcg
    12481 cctcctggcc gtcgtaggcg gtgcgcactt cgtgtcccgc gatgcgcagc atgacggcga
    12541 ggctgtcggc ggcgtcgcgg ttgtcgtccg ccaccaggat gcgatgccgc gcgccgcccg
    12601 cgggcggcgc cggcgccgcg gccgccgccg cggccggctc ggcggcgtgc tgcggcagcc
    12661 gcacgacgaa ctccgatccc ttgccggggc cgtcgctgcg cgcgagcacc gtgccgccgt
    12721 gcagctcgac caggcgcttc accagcgtga gcccgatgcc gagcccgccc tgcgagcgct
    12781 cgggcgagtg cggcacctgg gtgaacattt cgaacacgcg cgagagcatg tcgggcggga
    12841 tgcccaggcc ggagtcgcgc accgagatct cgacctggcc gtcgacgcgg cgcgcgccga
    12901 tgccgatgcg gccgccgggc ggcgtgtact tgaccgcgtt ggagagcagg ttgcagatca
    12961 cctgcaccag gcgcgtcggg tcgcaggtca gcgtgaccgg ctcctgcggc agctcgaccg
    13021 cgagtcggtg gcgcgactgc gccaccgccg ggccgcacac gtcgagcgcg ccctgcacga
    13081 gggtggcgag ctcggcgcgc tcgtgcttga gctcgatgcg cccgcggctg acgcgcgaaa
    13141 gatcgagcag gtcgtcgatc agccgcacca tctgcgccac ctggcgctcc atcagcgcgc
    13201 gcgcccagct cgccgcctcg cggtcgtgcg gcttgaggcg catcgcctcg agcgcgttgc
    13261 gcagcggcgc gagcgggttg cgcagctcgt gcgccaggat cgcgaggaac tcgtccttgc
    13321 gccggtcggc ttcgagcagc gcggcggcga cgcggttgtt ctccgacagg tggtcgcgga
    13381 tctggtactg ccggcggcgc gcgcgcagcg ccgcgcgcac cgagctcacc agcgccggca
    13441 cgcgcaccgg ccgctcgagc agcgtcacgt tgcccagggt ctcgagcgtg cgcagcacga
    13501 cttccgagtc ggcgccctct tcggtgagga tcagcagcgg gaagtccgac cacgccggct
    13561 ggcggcggat cgcgacgcgc agcatctgca ggctctcggc ggtgagcgcc tcctcgccga
    13621 gcatcgcaat gccggcgccg cggtcgatgc cctcgcagag ctccgcgacg tccgcgcaca
    13681 gcgcgcattc gacgcccgct tcctcgagga tcttgcggca cagcgccgcg tccttggccg
    13741 tcggcgcgag gatcaggacc gcctcgtcgc gcaacccccg ctcagcgtct tgcaagatcc
    13801 gcttcgtcct ttcccgcgaa ccccgggatt cccgacatga tgccgtggaa ctcggcgagc
    13861 ggcgggccga gcgcgacgcc gccgcgccgc atgctgagct cccgcagcgt gtcctcgtgc
    13921 gcgccgctgc gcttcttcac caccgagatc gcgcgccgca gcttgccctt cgcctcgaaa
    13981 tagcgcagcg cgatcacggt gtcggcgagg tagctcgcgt ccaccggcgt ctgcatgccg
    14041 ctgccgagca gtcccttctg cgccaccacc atcagcgtca ccacgccctg ctggccgagg
    14101 taggtcagca tttcgtgcag ctggatggtg aggaagcgct cctccggcat ggcgttgagg
    14161 tagccgttca ggctgtcgat cacgacgacg cgcgcggctt cttcctcgac cgcgcggcgg
    14221 atgccgtaga tgaattcgcc cggcgagagc tcgccgggat ccacctgctg cacgcggatg
    14281 cgcccggcct cgacgtggcg cttgagcccg atgccgagcg cctgcgagcg catcagcagc
    14341 gtgccgacgc tctcgtcgaa gatgtagagc accgcgcgct cgccgcgctc ggcagcggcc
    14401 accgcgtact gcgtggcgag cgtggacttg ccggcgccgg ccggccccat gagcagcgcg
    14461 ctggttccgc ggtgcaggcc gccgccggtc agggcgtcga gcgactcgac gccgctcttc
    14521 agcggctcgg cgtcgaagct gcggcggtgc tcggcggaga ccagccgcgg gaacacctcc
    14581 aggccgccgg tgcggatcat gaagtcgtgg tagccgccgc ggaacgcgcg cccgcgcatc
    14641 ttcatcacgc gtaggcggcg ccgctcgccg ccgtactcct gcgccatgtg cgagagcgag
    14701 atcacgccgt gggcgaggct gtggagctgc aggtcgttgt cggtcgcggt gcggtcgtcg
    14761 agcagcagca cggtgcaggc gcggccgatg aagaactgct tcagcgccag cacctgccgc
    14821 cggtagcgca gcgggtgctg cgcgagcagc cgcatctccg agagcgaatc gaacaccacg
    14881 cgcgagggct tgcgccgctc gacctcggcg aggatcgcgc gcgtggtgtc ggaaagctcg
    14941 acctccgagg ggtggaacag gcgcgactgg gcgtccggca gaaggctctc gtccgagggc
    15001 agcagctcgt ggcaatggat gccctcgagg ctccagccgt gcgagcgcgc cacgccgtcg
    15061 agctcctcct tggtctcgga gagcgagacg tacatgccgc tttcgccggc gcgtcggcct
    15121 tcgagcaggt actggagcgc gagcgtggtc ttgccggacc ccgggtcgcc ctcgaccagg
    15181 tagatgcggt tcggcgtgaa gccgccgccg aggatgttgt cgaggccggc aatacccgtg
    15241 gcgacggcag gcgcgtccgt attcgcttgc atggatcgga gtgcttgtat ttttgcggcc
    15301 gcaatttagc agcgcgcacg gtcgcgctca gccgcagccg acgctaattt cgcgtagtgg
    15361 gtttgctaca gcgctgtcgt tcgcttacgg aggcgtgagc ttcgcggggg agatcagcac
    15421 gccgaactgc tcgcaccaga ccaccacgct gccgaacttc ccgacatcga cgcccaccgg
    15481 aacgtcgtag ttctggctgc ctttgaacgc cttgaggcgc cccaggtcga cgaacatggt
    15541 gcgcgccacg ttggtcgagg gcatcacctc tttctccggc accaggtaga cgtggtattt
    15601 gggccccggc ccgacctcga agtcgggctc gaggtaaagc aggccgtcgt agaccgtgac
    15661 cttgcccttg ccgtagtgga tcgggtcgga gggattcgcg tgcacgaagc tgccgcgcgc
    15721 cagcaccttg cgctcgggct ggcccgccgg cgccggctgc accttctcgg tggcgacgat
    15781 gtcggcgagg aacacgtacg ggtagacgaa gatgccgagc gaggtgccgc cgatgaggcc
    15841 cagcaccgcg
//
LOCUS       DASRIE010000014         9044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1574241, whole genome shotgun sequence.
ACCESSION   DASRIE010000014 DASRIE010000000
VERSION     DASRIE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574241"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFB53_00620"
     CDS             <1..186
                     /locus_tag="VFB53_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85263.1"
                     /translation="FASRIESANKAARTRFLISSDTYRHVKERVAANRLEGVALPGRT
                     HRCTLYEVTGIIAPHAA"
     gene            225..611
                     /locus_tag="VFB53_00625"
     CDS             225..611
                     /locus_tag="VFB53_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012339858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN85264.1"
                     /translation="MTKTEYERLAHFRYQLRRFLRFSEELTRGNGITALQYQLMLQVK
                     GFPGRHWATVGELAERLQAQQHGVVSLISRCEAGGLVKRRASRADKRRVEIRLTPKGE
                     KLLARLARLHRLELRSLRDVAELNPR"
     gene            complement(598..1566)
                     /locus_tag="VFB53_00630"
     CDS             complement(598..1566)
                     /locus_tag="VFB53_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85265.1"
                     /translation="MRRLVLAGAGHAHAVALRAFAAKRPGGRIVLVAPEASVLYSGML
                     PGVAAGHYRPDECRIDFARLARAAGAEFIGDAVLGLDAVSRTLRLASGNHLTYDLLSL
                     NVGSAVAAPPGALTVKPFAAFLDAVRHAPRVAVVGAGTAGVEIAMALRHRGAQVTLST
                     DRFPFDGRVARRISAALERSGVEFRIGPPGEGVQVWASGARAYPWLKDSGLSTDAAGF
                     VLVDATLRSVAHPEVFAAGDCATLRTAPHAKSGVYSVRHGAVLAGNLSGGFRTYVPQP
                     RQLALITCGARYAIASWGAFSAEGAWAWRWKDRIDRRWVAGFASAG"
     gene            complement(1563..3197)
                     /locus_tag="VFB53_00635"
     CDS             complement(1563..3197)
                     /locus_tag="VFB53_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN85266.1"
                     /translation="MADRYGELYAKFRWNVPPRYNIAQACCGQWAPDRSRFALYWEDE
                     SGATAAYSFRDIQLAANRLSNALAALGVQRGDRVALLLPQRPESAIAYMAIFQMGAVA
                     LPLSHLFGPDALEYRMEHAGASVAIVEPTTIANLWAVRGKLSQLRHVIGVGGAQEAGV
                     HAWEALLAKSSSRFSLVDTAADDPAVVIYTSGTTGAPKGAYEAHRLLIGNLSGFVHSH
                     DFFPQRGDMFWSPADWAWAGGLFDALLPSWAFGIPILGYRGKFDAEKAYYLLQKYGVR
                     NSFLFPTALKLMMKAVPEPRKKYDLNLRSIMSAGESVGVTVIEWAKEELGVTINEMFG
                     QTEINYVVGNCQAAWPVKPGSIGRPYPGHRVAVIDEQGRERPRGELGEIAVNRNCNGE
                     ADPVFFLEYWKNPQATRDKFVGDWGLTGDQGKMDEDGYLWYQGRSDDVIKSSGYRIGP
                     AEIESCLVKHPAVANAAVIGKPDATRGSIVKAFIVLQPGQQASKALEEDIQKHVRGRL
                     APYEYPKEIEFIDALPMTTTGKVQRKELRKREEARG"
     gene            3221..3865
                     /locus_tag="VFB53_00640"
     CDS             3221..3865
                     /locus_tag="VFB53_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZN85267.1"
                     /translation="MAPLALVFDAYGTLYDVHAVVARCEEFWPGRGAELSLLWRTKQL
                     EYTWQRSLMRRYAPFSQVTLEALQYACAGLGLPLEGYQSAALLQEYRRLAVFPEVAGA
                     LARFATRKAILSNGSPDMLDPLVKQSGLAFDAVLSVDALKLFKPAPEVYQLAVDRLGV
                     VKEAIGFVSSNCWDALGAKAFGFRVWWINRLQAPVDALGLQPDAVVGSLDEILL"
     gene            3849..4382
                     /locus_tag="VFB53_00645"
     CDS             3849..4382
                     /locus_tag="VFB53_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN85268.1"
                     /translation="MKFCSNCAAPLLRRVPPGDNLPRWVCDGCGEIHYQNPKLVVGAV
                     AEYEGRLLLCRRAIEPRYGYWTLPAGFMENEETAGQAALRETLEEAGARIELLAPYAL
                     LSVPYVNQVHLFYRARLLDLEFKPGEESLEVTLVGEAQLPWKEIAFRTVGLTLRHWFA
                     DRAKGDFGFHAEDLALR"
     gene            4408..5691
                     /locus_tag="VFB53_00650"
     CDS             4408..5691
                     /locus_tag="VFB53_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN85269.1"
                     /translation="MRKTLLVPLLAYAPFAGAADAKLDPANTAWMITASVLVLFMTLP
                     GLALFYAGLVRVKNVLSVLMQCFAITCVVTLAWVVVGYSIAFGDGGAANAWFGGFGKS
                     FLAGIDVKTVKGSIPETVFAMFQLTFAIITPALVVGAYAERVRFGGMLVFSVLWLLFV
                     YCPIAHWVWGDGWLQKMGVMDFAGGLVVHLNAGMAALVCALVLGRRRGFPDTAMPPHN
                     MTMAVTGACMLWVGWFGFNAGSALAADGAAGMAMLVTHIGAATGSLAWLVCERIRYGK
                     PSVLGICTGMVAGLGTITPASGFVGPLGAIAIGGAAGVICFFATSYLKRRLNVDDSLD
                     VFPVHGVGGILGTLLTGVFVSAALGGMGYPDKVAMGGQLAVQFTGVLAVGAWSGIVTW
                     ILLKLTDVVAGLRVTADEETEGLDTALHNEKGYNL"
     gene            5696..6391
                     /locus_tag="VFB53_00655"
     CDS             5696..6391
                     /locus_tag="VFB53_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN85270.1"
                     /translation="MRRREFIQVCAAAAAAAGSPAFAADARPRFYGKVRLVDEGGAPL
                     RAKAIPAHRNLIFHYPYASTPCFLLNLGRPAKASAQLKTADDQPYEWRGGVGAQRSIV
                     AYSAICAHRLSYPTRDISFISYRAEKSARNRFANVIHCCSEHSQYDPAEGGRVLAGPA
                     PQPLAAILLEHDPASDQLTAVGTLGGEMFAEFFAKYEFRLALDNGDGVRRAMQGKAVV
                     TELENYCKQQVKC"
     gene            6385..7092
                     /gene="aat"
                     /locus_tag="VFB53_00660"
     CDS             6385..7092
                     /gene="aat"
                     /locus_tag="VFB53_00660"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZN85271.1"
                     /translation="MLTWLRPGERFPPVEQALKDPNGLLAAGGELSPARLLEAYRHGI
                     FPWYSGGEPILWWSPDPRMVLYCAELRVSRSLAKSARNKGYELRIDTAFREVLAGCAG
                     PRQGDGGTWLGEDMRKAYAALHRAGHAHSFETWRKGELVGGLYGVALGRMFYGESMFS
                     RAADASKVALLGLVAELRRRGCPLIDCQVRTPLLASLGAREIARPAFLREIAALVNYA
                     EAPGKWTRRSEPDVPAE"
     gene            7076..7825
                     /locus_tag="VFB53_00665"
     CDS             7076..7825
                     /locus_tag="VFB53_00665"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZN85272.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTD
                     LYGDLVKSGFRRSGIFTYRPHCDSCRACVPVRIPVAEYVASRSQRRAWKQHAGLAAQV
                     QPLKFRLEHYALYLRYQSRRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFREQGQLAM
                     VSIVDYLPDGLSSVYTFFDPERRHASFGTYNVLWQIEACRALGLPYLYLGYWIRESPK
                     MAYKSRFMPIEGFTGGGWRRLAEADLARMRF"
     gene            7828..8844
                     /locus_tag="VFB53_00670"
     CDS             7828..8844
                     /locus_tag="VFB53_00670"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZN85273.1"
                     /translation="MLYALARPLLFALDAERAHELTLGLADAPLRFRLLRVPRAPGAP
                     VRVMGLDFPNAVGLAAGLDKNAEYVDALAQLGFGFLEVGTVTPRPQPGNPRPRLFRLP
                     RAQALINRMGFNNVGLDAFLENLARASFRGVLGINIGKNADTPIERAADDYALCLERV
                     YPRASYVTVNVSSPNTQNLRALQEDRALGALLSRLAALRDQLAERHGRRVPLALKVAP
                     ELEAAQLQGIADAVRRHRIDAVIATNTSLSREGVGSLPHAAEAGGLSGAPIRARATAT
                     LAALASALKGEAALIGVGGILRGSDAAEKLAAGASLVQLYTGLVYRGPVLVAECVTSA
                     RASR"
     gene            8775..9044
                     /locus_tag="VFB53_00675"
                     /pseudo
     CDS             8775..9044
                     /locus_tag="VFB53_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
BASE COUNT         1297 a         3224 c         3128 g         1395 t
ORIGIN      
        1 tttgccagca ggatcgaatc tgcgaacaag gcagccagga ccaggttcct gatctccagc
       61 gacacttacc gccacgtcaa ggagcgtgtc gcggccaaca ggctcgaggg cgtcgcgttg
      121 cccggcagga cgcaccgatg caccctgtac gaggtgaccg ggatcatcgc gcctcacgcg
      181 gcgtagccag ttatatcata gtgcgatata taatcgcgcc gcccatgacc aagaccgaat
      241 acgaacggct tgcccacttc cgctaccagc tgcgccgctt cctgcgcttc agcgaggagc
      301 tgacgcgcgg caacggcatc accgccctgc aataccagct gatgctgcag gtgaagggct
      361 tccccggcag gcactgggcg acggtcggcg agctggccga gcgcctgcag gcgcagcagc
      421 atggcgtggt gtccctgatc tcgcgctgcg aggcgggcgg gctggtgaag cgccgcgcga
      481 gccgcgcgga caagcgccgg gtggaaatca ggctcacgcc gaagggggaa aagctccttg
      541 cgcgcctggc gcgcctgcac cgcctggaac tcaggtccct gcgcgacgtg gccgagctca
      601 acccgcgcta gcgaagccgg cgacccagcg ccggtcgatg cggtccttcc agcgccaggc
      661 ccaggcgcct tcggcgctga acgcgcccca gctcgcgatc gcgtagcgcg cgccgcaggt
      721 gatgagcgcg agctggcgcg gctggggaac gtaggtgcgg aatccgccgg aaaggttccc
      781 ggccagcacc gcgccgtgcc gcaccgagta aacacccgat ttcgcatgcg gcgcggtgcg
      841 caaggtggcg cagtcgccgg cggcgaacac ttccgggtgc gcgacgctgc gcagcgtcgc
      901 gtccaccagc acgaagcccg cggcgtccgt cgacagaccc gaatccttca gccacggata
      961 ggcgcgtgcg ccagaggccc agacctgcac gccctctccc ggcggcccga tcctgaactc
     1021 gacgcccgag cgctccagcg cggcgctgat gcgccgcgcg acgcgcccgt cgaacggaaa
     1081 ccggtcggta ctcagcgtca cctgggcgcc gcgatggcgc agcgccatcg cgatctccac
     1141 gccggccgtg cccgcgccga ccacggcgac gcgcggcgcg tggcgcaccg cgtcgaggaa
     1201 cgccgcaaag ggcttcaccg tcagcgcgcc cggcggcgcg gccactgcgg acccgacgtt
     1261 gagcgagagc aggtcgtagg tcaggtgatt gcctgaggca agccgcagcg tcctcgacac
     1321 ggcgtccagg ccgagaaccg cgtcgccgat gaactccgcg cccgcggcgc gcgcgagccg
     1381 cgcgaagtcg atcctgcact cgtcggggcg gtagtgcccg gccgcgacgc cggggagcat
     1441 gccggagtaa aggacggacg cctcgggcgc gacgagcacg attctgccgc cgggcctctt
     1501 cgcggcgaag gcccgcagcg cgacggcatg cgcatgaccc gcgccggcga ggacgagccg
     1561 cctcacccgc gcgcttcttc gcgcttgcgc agctccttcc tctgcacctt gccggtggtg
     1621 gtcatgggca gcgcgtcgat gaactcgatc tccttcgggt actcgtaggg cgcaagtcgc
     1681 ccgcgcacgt gcttctggat gtcttcctcc agcgccttgg aggcctgctg ccccggctgc
     1741 agcacgatga acgccttgac gatcgagccg cgcgtcgcgt ccggcttgcc gatcaccgcg
     1801 gcattggcca ccgccgggtg cttcaccagg cagctttcga tctccgccgg cccgatgcgg
     1861 tagcccgagc tcttgatgac gtcgtccgag cggccctggt accagaggta gccgtcctcg
     1921 tccattttgc cctggtcccc ggtgaggccc cagtcgccga cgaacttgtc cctggtggcc
     1981 tgcggattct tccagtactc caggaagaac acggggtccg cctcgccgtt gcagtttcgg
     2041 ttgaccgcga tctcgcccag ctcgccgcgc ggccgctcgc ggccctgctc gtcgatcacc
     2101 gcgacgcggt gccccggata cgggcggccg atcgagccgg gcttcaccgg ccaggcggcc
     2161 tggcaattac ccaccacgta gttgatttcg gtctgcccga acatctcgtt gatggtgacg
     2221 ccgagttctt ccttggccca ttcgatcacc gtgacgccga ccgactcgcc cgcgctcatg
     2281 atcgagcgca ggttcaggtc gtacttcttc cgcggctcgg gcaccgcctt catcatcagc
     2341 ttcaaggcgg tggggaagag gaacgaattt cgcacgccgt acttctgcag caggtaatag
     2401 gctttttccg cgtcgaactt gccgcggtag ccgagaatcg ggatgccgaa ggcccaggag
     2461 ggcagcaagg cgtcgaacag gccgcccgcc caggcccagt cggcgggcga ccagaacatg
     2521 tcgccgcgct ggggaaagaa gtcgtgcgag tgcacgaagc cgctgagatt gccgatcagc
     2581 agccggtgcg cctcgtaggc gcccttgggc gcgcccgtgg tgccgctggt atagatgacc
     2641 accgccggat cgtcggccgc ggtgtccacc aggctgaagc gcgagctcga tttcgccagc
     2701 agcgcctccc aggcgtgcac gccggcctcc tgcgcgccac ccacgccgat cacgtggcgc
     2761 agctgcgaga gcttgccgcg caccgcccac aggttggcga tggtggtcgg ctcgacgatg
     2821 gccacgctcg cgccggcatg ctccatgcgg tattcgagcg cgtcgggccc gaacaggtgc
     2881 gacagcggca gcgccacggc gcccatctgg aagatcgcca tgtaggcgat cgccgactcg
     2941 gggcgctgcg gcagcagcag cgcgacgcgg tcgccccgct gcacgcccag cgcggccagc
     3001 gcgttcgaca ggcggttggc ggcgagctgg atgtcccgga agctgtaggc cgcggtggcg
     3061 cccgactcgt cctcccagta cagcgcgaag cgcgagcggt ccggcgccca ctgcccgcag
     3121 caggcctgcg cgatgttgta gcgcggcggg acattccagc ggaatttcgc gtagagctcg
     3181 ccgtagcggt cggccatcgc gcgcattcta tacttcgccc atggcgcccc tcgccctggt
     3241 gttcgacgcg tatggcacgc tctacgacgt gcacgcggtg gtggcacgct gcgaggagtt
     3301 ctggccgggg cgcggcgcgg aactgtcgct gctgtggcgc acgaagcagc tcgagtacac
     3361 ctggcagcgc agcctgatgc ggcgctacgc gccgttctcg caagtgaccc tggaggcgtt
     3421 gcagtacgcc tgcgccgggc tcgggctacc gctggagggc taccaatccg ccgcgttgct
     3481 gcaggagtac cgcaggctcg ccgtgtttcc cgaggtggcc ggcgccctgg cgcgcttcgc
     3541 cacccgcaag gccatcctgt ccaacggctc gccggacatg ctcgatccgc tggtgaagca
     3601 atccggcctg gcgttcgacg cggtgctgag cgtggacgcc ctgaagctgt tcaagcccgc
     3661 gcccgaggtc taccagctcg cggtggaccg gctcggcgtg gtgaaggagg caatcggctt
     3721 cgtttcctcc aactgctggg acgcgctcgg cgccaaggcc ttcggcttcc gcgtctggtg
     3781 gatcaaccgc ctccaggcgc cggtggacgc cctgggtctg cagcccgacg cggtcgtcgg
     3841 ctcgctcgat gaaattctgc tctaactgcg ccgcgccgtt gctcaggcgc gtcccgcccg
     3901 gggacaacct gccgcgctgg gtgtgcgacg gctgcggcga gatccattac cagaacccca
     3961 agctggtggt gggcgccgtc gcggaatacg agggcaggtt gctgctgtgc cgcagggcca
     4021 tcgagccgcg ctacggctac tggacgctgc ccgccgggtt catggaaaac gaagagaccg
     4081 ccggccaggc ggcgctgcgc gagacgctgg aggaggccgg ggcgcgcatc gagctcctgg
     4141 cgccctatgc gctgctctcg gtgccctacg tcaatcaggt gcacctgttc tatcgggcgc
     4201 ggctgctgga cctcgagttc aagccgggcg aggaaagcct ggaagtgacc ctggtgggcg
     4261 aggcgcagct gccctggaag gagatcgcct tccgcaccgt gggcctgacc ctgcggcact
     4321 ggtttgccga ccgggccaag ggcgactttg gctttcacgc cgaggacctc gcgctccgct
     4381 agaatccgca ccaaagggga ggcaaccatg agaaaaactc tgctggtccc gctgctcgcg
     4441 tacgcgccgt tcgccggcgc cgccgacgcg aagctcgatc cggccaacac cgcctggatg
     4501 atcaccgcct cggtgctggt gctgttcatg acgctgcccg gcctggccct gttctacgcc
     4561 ggcctggtgc gcgtgaaaaa cgtgctctcg gtgctgatgc agtgcttcgc catcacctgc
     4621 gtggtgacgc tggcctgggt ggtggtgggc tacagcatcg ccttcggcga cggcggcgcg
     4681 gccaacgcct ggttcggggg gttcggcaag tcgttcctcg ccggcatcga cgtcaagacg
     4741 gtcaagggca gcatcccgga aaccgtgttc gccatgttcc agctcacctt cgccatcatc
     4801 accccggcgc tggtggtcgg cgcctacgcc gagcgcgtgc gcttcggcgg gatgctcgtg
     4861 ttcagcgtct tgtggctgct gttcgtctac tgcccgatcg cccactgggt gtggggcgac
     4921 ggctggctgc agaagatggg cgtgatggac ttcgccggcg gcctggtggt gcacctgaac
     4981 gcgggcatgg cggcgctggt ctgcgcgctg gtgctcggcc ggcgccgggg ttttccggat
     5041 acggcgatgc cgccgcacaa catgaccatg gcggtcaccg gggcctgcat gctgtgggtg
     5101 ggctggttcg gcttcaacgc cggcagcgcg ctcgcggccg acggcgcagc cggcatggcg
     5161 atgctggtga cgcacatcgg cgcggccacc ggctcgctcg cctggctggt gtgcgagcgc
     5221 atccgctacg gcaagccttc ggtgctgggc atctgcaccg gcatggtggc tggattgggc
     5281 accatcaccc ccgcgtcggg attcgtcggc ccgctgggtg cgatcgccat cggcggcgcg
     5341 gcgggcgtga tctgcttctt cgccaccagc tacctcaagc gccggctgaa cgtggacgac
     5401 tcgctcgacg tgttcccggt gcacggcgtg ggcggcatcc tcggcaccct gctcaccggc
     5461 gtgttcgtgt cggcggcgct gggcggcatg ggctacccgg acaaggtggc gatgggcggc
     5521 cagctggcgg tgcagttcac cggcgtgctc gccgtcggcg cctggtcggg catcgtcacc
     5581 tggatcctgc tcaagctgac cgacgtcgtg gccggactgc gcgtcacggc tgacgaggag
     5641 accgagggcc tggacacggc gctgcacaac gagaagggct acaacctcta gcggcatgcg
     5701 gcgccgggag ttcatccagg tctgcgcggc ggcggcggcc gccgcaggat ccccggcctt
     5761 cgcggccgat gcccggccgc gcttctacgg caaggtcagg ctggtggacg aaggcggcgc
     5821 gccgctgcgc gccaaggcga tccccgcgca ccgcaacctg attttccact acccctacgc
     5881 ctcgacgccc tgcttcctgc tcaaccttgg gcgccccgcc aaggcctcgg cgcagctgaa
     5941 gaccgccgac gaccagccct acgagtggcg cggcggcgtc ggcgcgcagc gatcgatcgt
     6001 cgcctactcg gcgatctgcg cgcaccggct cagctatccg acgcgcgaca tcagcttcat
     6061 cagctaccgc gcggaaaaaa gcgcgcgcaa ccgcttcgcc aacgtgatcc actgctgctc
     6121 cgagcacagc cagtacgacc ccgccgaagg cggccgcgtg ctcgccggtc cggcgccgca
     6181 gccgctggcc gcgatcctgc tcgagcacga cccggcgagc gaccagctca ccgcggtggg
     6241 cacgctcggc ggcgagatgt tcgccgagtt cttcgccaag tacgagttcc gcctcgcgct
     6301 ggacaacggc gacggggtgc gccgcgcgat gcagggcaag gccgtggtga ccgaactcga
     6361 gaactactgc aagcagcaag tgaagtgctg acatggctca ggcccggcga gcgctttccg
     6421 ccggtcgagc aagccctgaa ggatcccaac ggactgctcg ccgccggcgg cgagctgtcg
     6481 ccggcgaggc tgctggaggc ctaccgccac ggcattttcc cctggtattc gggcggcgag
     6541 ccgatcctct ggtggtcgcc cgacccgcgc atggtgctgt attgcgcgga actgagggtc
     6601 agcaggtcgc ttgccaagag cgcgcgcaac aagggctacg agctgcgcat cgacaccgcc
     6661 ttccgcgagg tgctggccgg ctgcgccggc ccgcggcagg gagacggcgg cacctggctg
     6721 ggcgaggaca tgcgcaaggc ctatgccgcc ctgcaccgcg ccgggcacgc gcattccttc
     6781 gagacctggc gcaaggggga gctggtcggc ggcctgtacg gggtggcgct ggggcgcatg
     6841 ttctacggcg aatccatgtt ctcgcgcgcc gccgacgcct ccaaggtcgc gctgctcggg
     6901 ctggtggccg agttgcggcg ccgcggctgc ccgctgatcg actgccaggt gcgcacgccg
     6961 ctgctggcgt cgctgggcgc gcgcgaaatc gcgcggccgg cctttttgcg cgaaatcgca
     7021 gcgttggtaa actacgccga ggccccaggg aaatggaccc gacgatcgga acccgatgtc
     7081 ccggctgaat gacctgccgc atgcggtact gcagttctac gtcaccgcgc cgtacccctg
     7141 cagctatctg ccggaccgca tggcgcgctc gcaggtcgcc acgccctcgc acctgatcaa
     7201 caccgacctg tacggcgacc tggtcaagtc gggcttccgc cgcagcggca tcttcaccta
     7261 ccggccgcac tgcgacagct gccgcgcctg cgtgccggtg cgcatcccgg tggccgagta
     7321 cgtcgccagc cgctcgcagc gccgcgcctg gaagcagcac gccggcctcg ccgcgcaggt
     7381 gcagccgctc aagttccgcc tcgagcacta cgcgctgtac ctgcgctacc agtcgcgccg
     7441 ccattccggc ggcggcatgg acaacgacag ccgcgaccag tattcgcatt tcctgctgca
     7501 gagccgcgtc gacacgcggc tggtggaatt ccgcgagcag ggccagctgg cgatggtgtc
     7561 gatcgtcgac tacctgcccg acggcctgtc ctcggtgtac accttcttcg acccggagcg
     7621 gcgccacgcc agcttcggca cctacaacgt gctgtggcag atcgaggcct gccgcgccct
     7681 cggcctgccc tatctctacc tcggctactg gatccgcgag agcccgaaga tggcctacaa
     7741 gtcgcgcttc atgccgatcg agggcttcac cggcggcggc tggcggcggc tcgccgaagc
     7801 ggacctcgcg cgcatgcgct tctgagcatg ctgtatgcgc tggcgcgccc cctgctcttc
     7861 gccctcgacg cggagcgcgc ccacgaactg accctggggc tggcggacgc gccgctgcgt
     7921 tttcgcctgc tgcgcgtgcc gcgcgcaccc ggcgcgccgg tgcgcgtgat gggactggac
     7981 ttccccaacg ccgtcggcct cgccgcgggc ctggacaaga acgccgagta cgtcgacgcg
     8041 ctggcgcagc tcggtttcgg cttcctcgag gtgggcaccg tcacgccgcg gccgcagccg
     8101 ggcaacccgc ggccgcgcct gttccgcctg ccgcgcgccc aggcgctgat caaccgaatg
     8161 ggcttcaaca acgtcgggct ggacgcgttc ctcgagaacc tcgcgcgcgc cagctttcgc
     8221 ggcgtgctcg gcatcaacat cggcaagaac gccgacacgc cgatcgagcg cgcggccgac
     8281 gattacgcgc tgtgcctgga gcgcgtctat ccgcgcgcca gctacgtgac ggtgaacgtg
     8341 tcctcgccga acacgcagaa cctgcgcgcg ttgcaggagg accgcgcgct cggcgccctg
     8401 ctctcgcgcc tggcggcgct gcgcgatcag ctcgccgagc gccacggccg ccgcgtgccg
     8461 ctggcgctga aggtggcgcc cgagctcgag gcggcgcagc tgcagggcat cgccgacgcg
     8521 gtgcgccgcc accgcatcga cgccgtcatc gccaccaata cctcgctgtc gcgcgagggc
     8581 gtcgggagcc tgccgcacgc cgccgaggcg ggcgggctgt cgggcgcgcc gatccgcgcg
     8641 cgcgccaccg ccacgctcgc cgcgctggcg tccgccctga agggcgaggc ggcgctgatc
     8701 ggcgtgggcg gcatcctgcg cggcagcgac gcggcggaga agctcgccgc cggggcctcg
     8761 ctggtgcagc tctacaccgg actcgtctac cgcgggcccg tgctggtggc cgaatgcgtg
     8821 acaagcgcgc gcgcgtcgcg ctgatcgacg aggcgcgctg catcggctgc gcgcgctgcc
     8881 tcgacgcctg cccggtggac gcgatcgccg gcgccggcgg gctgatgcac acggtgatcg
     8941 ccccgttgtg catcggctgc gacctgtgcc tgccgccctg ccccgttgac tgcatcgcca
     9001 tggtgcccgc cccggcgccc tggaccacgg cggacgccgc ggcg
//
LOCUS       DASRIE010000015        12011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2095434, whole genome shotgun sequence.
ACCESSION   DASRIE010000015 DASRIE010000000
VERSION     DASRIE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095434"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFB53_00680"
     CDS             complement(<1..509)
                     /locus_tag="VFB53_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN85274.1"
                     /translation="MNDPSRPSLLERLSSLLMREPGDREQLVHLLRSAYSRNLLDADA
                     LSMIEGALTVSEMQVRDIMVPRAQVDFIDINEPVEKFIPQIISTAHSRFPVIDQNRDD
                     VIGILLAKDLLRHYAAEEGVNVREMLRPAVFVPEAKRLNVLLREFRASRNHMAIVIDE
                     YGGVAGLVT"
     gene            complement(532..921)
                     /gene="ybeY"
                     /locus_tag="VFB53_00685"
     CDS             complement(532..921)
                     /gene="ybeY"
                     /locus_tag="VFB53_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZN85275.1"
                     /translation="MPRRGVPGARSFEKWLPPLGARRVTVRIVGEAEGRRLNRSFRKK
                     NHATNVLSFAYGGGHGDVVLCHPVIAREARAQGKSLAAHYAHLALHGLLHLRGYDHER
                     KREALRMERAEIRLLKRAGFRNPYAVE"
     gene            complement(941..1894)
                     /locus_tag="VFB53_00690"
     CDS             complement(941..1894)
                     /locus_tag="VFB53_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZN85276.1"
                     /translation="MPPKPVEVRLEPVDNRRLARLCGALDANLRQIESALDVSIARRG
                     ARFTVRGAAQQAARAAQALEHFYGKADAELSVDDVQLGLTELSRGAAAAAQDSATPLK
                     TRRADLHGRTPRQVQYIEQILAHDITFGVGPAGTGKTYLAVACAVDALERDKVKRIVL
                     VRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKTGKLLERGTIELAPLAY
                     MRGRTLNHSFIILDEAQNTTPEQMKMFLTRIGFGARAVVNGDVTQIDLGRGQKSGLIE
                     ARRILAGVRGIAFTQFGAQDVVRHPLVARIIDAYDKQAQEE"
     gene            complement(1884..3212)
                     /gene="miaB"
                     /locus_tag="VFB53_00695"
     CDS             complement(1884..3212)
                     /gene="miaB"
                     /locus_tag="VFB53_00695"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZN85277.1"
                     /translation="MAGKLYLRTFGCQMNEVDSASIARLLEQTEGLRPAEKPEDADLI
                     VFNTCSVREKAQEKVFADLGRVKHLKRENPALMIAVGGCVASQEGAGIVERAPFVDVV
                     FGPQTLHRLPALLARRRDTGKPQVDVSFPEIEKFDQLPAPRKEGAAAFLTIMEGCSKY
                     CSFCVVPYTRGAEVSRPFEDVVREAAALAAQGVKELTLLGQNVNAWRGDIGGAPADFA
                     ELLRYVAEIDGVERIRYTTSHPREFTQRLVDAHAELGALAPHVHLPVQSGADRVLAAM
                     KRGYTALEYRSIVRRLRQARPDVSLSSDFIVGFPGESAADFEATLRLARELEFDASFS
                     FLYSPRPGTPAAELPDPTPREEKLERLQRLQAQLESQAAAVSAAMIGSTQRVLVEGPS
                     RKNPAELAGRTGNNRIVNFPAAAEVGGAYAEVRITAALAHTLRGELVDAA"
     gene            complement(3252..3328)
                     /locus_tag="VFB53_00700"
     tRNA            complement(3252..3328)
                     /locus_tag="VFB53_00700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3292..3294),aa:Met,seq:cat)
     gene            3394..4548
                     /locus_tag="VFB53_00705"
     CDS             3394..4548
                     /locus_tag="VFB53_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HZN85278.1"
                     /translation="MKTDVAIVGAGLAGLALARALRGLAVSLVAPERAAPPPQGWDAR
                     VYAITPGNAAFLADLRVWQALPAGRVAPVRAMRVFGDDGAAVLEFDAYRAGTDALAWI
                     VEDGRLREALWAALAGRDDLQVLAPAQCARLRLDARRALLDLEDGRGIEARLVVGADG
                     ARSRVREQAGIAAADTPYAQSAVVANFACARGHGDTALQWFQGGPVLALLPLPGARVS
                     MVWSVDEAEAARLLALAPAALCREVARASGQALGELELITAPRAFPLRRLAARRTVAP
                     RVALAGDAAHVIHPLAGQGANLGLQDARALAAVLAGREPQRDPGEIGLLRRYERARAR
                     DVLAMDATVHGLYRLFGAAAGPAAGLRNAALNLADRLPVLKNLLIRQAIT"
     gene            4545..5282
                     /locus_tag="VFB53_00710"
     CDS             4545..5282
                     /locus_tag="VFB53_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZN85279.1"
                     /translation="MTRILVFLLGALLAAPGAWADEAKIRSVVEAALGGVRIEGVQPA
                     PVPGLFEVRFLSREGPQIVYTDAEANYILQGSLFDTRSKRDLTEERLSKLSAIKFESL
                     PLDLAVKVRRGNGKRVLAMFSDPYCPACRQFEQTLAQVDDITIYYFMYPVIRPELAEH
                     SKAVWCSPDRGKAWLALAARPKAAPPASNAGCDTPIERVLELGKTLRVNSTPTLFFAD
                     GERVRGGLRAADLRARLDQASRAAKVR"
     gene            complement(5279..6265)
                     /locus_tag="VFB53_00715"
     CDS             complement(5279..6265)
                     /locus_tag="VFB53_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HZN85280.1"
                     /translation="MRAFVAGCARAPLEPAMQAPCEAARSLPAGDEAAARAFFEGAFS
                     AYAIAAPDGAVEGTVTGYYEPVLEGSRQRSARYRQPVHGLPDDLVVVDLAAQYPELRG
                     MRLRGRLDGRRLLPYYSRGEIAAGNGAFAAPVIAWVADPVELFFLQIQGSGQIQLDTG
                     ERIRVGFAEQNGHPYRSLGRLLVERGELTLEQASMQGIKAWAAANPARYQEALNHNAS
                     YVFFRELPAAGGPIGALGVPLDAGYSIAVDPRYVPLGAPVFLATTYPLSPQPLERLVM
                     AQDTGGAIRGAQRADFFWGTGPEAGALAGRMRQPGRMWVLWPRGAPLPRPLP"
     gene            complement(6495..6878)
                     /gene="apaG"
                     /locus_tag="VFB53_00720"
     CDS             complement(6495..6878)
                     /gene="apaG"
                     /locus_tag="VFB53_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZN85281.1"
                     /translation="MAQEKRYDIAVSAATRYLAEQSDESAGRYAFAYTITMRNPGNVT
                     AQLISRHWIITDSQGLVQEVRGLGVVGAQPVLRPGESFEYTSGTSIATPVGTMRGSYQ
                     MVAEDGTRFEAPIPEFTLSVPRVLH"
     gene            6904..7563
                     /gene="rpe"
                     /locus_tag="VFB53_00725"
     CDS             6904..7563
                     /gene="rpe"
                     /locus_tag="VFB53_00725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZN85282.1"
                     /translation="MAFRIAPSILSADFARLGEEVRAVEAAGADLLHFDVMDNHYVPN
                     LTVGPLVCAALKPHTRLPIDVHLMVKPVDRIIPEFARAGAGIISFHPEASEHVDRTIG
                     LIKEQGCKAGLALNPATPLDCLDFTLDKLDLVLLMSVNPGFGGQQFIRAVLPKIAEVR
                     KRIARLGRHIWLEVDGGVKADNIAEIAQAGADTFVAGSAIFGAKDYAATIRDMRSRLV
                     V"
     gene            7721..9220
                     /gene="trpE"
                     /locus_tag="VFB53_00730"
     CDS             7721..9220
                     /gene="trpE"
                     /locus_tag="VFB53_00730"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZN85283.1"
                     /translation="MNELEFQKLAQQGYTRIPVLAEARADLYTPLAVYLKLAGGPYSY
                     LLESVVGGERFGRYSFIGLPCAERIEARGGTVRRLLRDARGADVCLEEAAGDPFAYVR
                     AYLKRHRVAPAPGALKFPGGLAGYFGYEMVRHVEPRALSGAKPDALGTPDMLLLVSDE
                     LAVVDNVMGKLHLVVYADPGQPDAWRTAQGRLERLRARLAAPLPESLVLAQPRAGNGP
                     ATLACSFTEADFLAAVGRCKEYVYAGDCMQVQVSQRTSREFRAPPIALYRSLRSLNPS
                     PYMFYFDFGDHHVVGASPEIMVRVAGDKITLRPIAGTRARGATPQDDARIAEELLADP
                     KERAEHVMLIDLGRNDVGRVAAVGSVKVTETMVVERYSHVMHIVSNVEGRCAPGMDAL
                     DVFKASFPAGTVTGAPKVRAMQIIDELEPVKRGVYSGAVGYLGFNGDMDVAIAIRTAV
                     LKDGRLHVQAAAGVVADSVPLSEWQETHHKARAILKAAEAAAADGAEAP"
     gene            9217..9789
                     /locus_tag="VFB53_00735"
     CDS             9217..9789
                     /locus_tag="VFB53_00735"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZN85284.1"
                     /translation="MILVLDNYDSFTYNLVQYLGELGAEVKVLRNDEATVDEVARLRP
                     EKVVISPGPCTPNEAGISLALIERLAGSVPILGVCLGHQAIGQAFGGKVVRAAQVMHG
                     KVSRVRHDGRGVFQGLPADFVATRYHSLAVERGTLPEALQVTAQAEDGEIMGLRHRAF
                     AVEGVQFHPEALLTEHGHKMLENFLKGERQ"
     gene            9786..10826
                     /gene="trpD"
                     /locus_tag="VFB53_00740"
     CDS             9786..10826
                     /gene="trpD"
                     /locus_tag="VFB53_00740"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZN85285.1"
                     /translation="MKQITIAEAIQRTVEHREVFHDEMLHIMRQIMRGELTPAQIAGF
                     ITGLRVKKETIGEIAAAAQVMREFATHVKVADARHLVDTCGTGGDAAHTFNVSTASAF
                     VAAAAGARVAKHMGRSVSSSSGSAEVLEALGANIALSPEQTGQAIDKLGLGFMFAPLH
                     HAAMKYAAPVRKELGVRTVFNILGPLTNPAGAPNQVMGVFHPDLVGIQVRALQRLGSQ
                     HVLVVYGLEGLDEISISGETLVGELANGEISEYTIHPQQFGIELYDRRAIQVNTVEES
                     RQMILAVLENQPGPALNIVLLNAGAALYAAGVAASMQAGIDLARRAIAKGDARRKLDE
                     FVAYTKSVAAKG"
     gene            10831..11634
                     /gene="trpC"
                     /locus_tag="VFB53_00745"
     CDS             10831..11634
                     /gene="trpC"
                     /locus_tag="VFB53_00745"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZN85286.1"
                     /translation="MADILQRILASKREELRAARAAVPPAEMARRARAAPPPRDFVGA
                     LRGRIAAGGAAVIAEVKRASPSQGLLRADFRPAAIAQSYARGGAACLSVLTDREYFQG
                     APEHLVQARAACALPVLRKDFMLDPYQVLEARALGADCILLIAAALAPAQMRELEEAA
                     QELGMAVLVEVHDAAELDAALALDTPLVGINNRNLRSFETRLETTLELGVRVPAPRLV
                     IAESGIGTRADVERLRNAGVRAFLVGEAFMRAKDPGNALAKLFEAGATT"
BASE COUNT         1741 a         4175 c         4333 g         1762 t
ORIGIN      
        1 atggtgacca ggcctgccac cccgccgtac tcgtcgatca cgatcgccat gtggttgcgg
       61 ctggcgcgga actcgcgcag cagcacgttc aggcgcttcg cttcgggcac gaacaccgcc
      121 gggcgcagca tctcgcgcac attgacgccc tcctcggcgg cgtagtggcg cagcaggtcc
      181 ttggcgagca ggatgccgat gacgtcgtcg cgattctggt cgatcaccgg gaagcgcgag
      241 tgcgcggtgg agatgatctg cgggatgaac ttctcgaccg gctcgttgat gtcgatgaag
      301 tcgacctggg cgcgcggcac catgatgtcg cgcacctgca tctccgagac cgtgagcgcg
      361 ccctcgatca tcgagagcgc gtcggcgtcg agcaggttgc gcgagtacgc cgagcgcagc
      421 aggtgcacca actgctcgcg gtcgcccggc tcgcgcatga gcagcgacga caggcgctcg
      481 agcaggctgg gtcgcgaggg gtcgttcatg cccggagtta tagccgcgga ttcattctac
      541 agcgtaagga ttacggaaac cggcgcgctt tagcaaccgt atctccgccc gttccatgcg
      601 cagcgcttcc cgtttccttt catggtcgta gccgcgcagg tgcagcaggc cgtgcagcgc
      661 caggtgcgcg tagtgcgccg ctaggctctt gccctgcgcg cgcgcctcgc gggcgatcac
      721 gggatggcac agcaccacgt cgccatggcc gccgccgtac gcgaaggaaa gcacgttcgt
      781 cgcgtggttc tttttcctga agctgcggtt cagccgccgg ccctcggctt cgccgacgat
      841 gcgcacggtc acccgccgcg cgcccagcgg cgggagccat ttctcgaagg agcgcgcgcc
      901 gggcacgccg cggcgcggca ctgcgtactg aaccgatacc ttattcttcc tgcgcttgct
      961 tgtcgtaggc atcgatgatg cgggccacca gcgggtggcg caccacgtcc tgcgcgccga
     1021 actgcgtgaa cgcgatgccg cgcacgccgg cgaggatcct gcgcgcctcg atgagcccgc
     1081 tcttctgccc gcggccgagg tcgatctgcg tgacgtcgcc gttcaccacc gctctcgccc
     1141 cgaagccgat gcgcgtgagg aacatcttca tctgctcggg cgtggtgttc tgcgcctcgt
     1201 ccaggatgat gaacgagtga ttcagcgtgc gcccccgcat ataggcgagc ggcgccagct
     1261 cgatggtgcc gcgctcgagg agcttgccgg tcttctcgaa tcccatcagg tcgtacagcg
     1321 cgtcgtacag cggccgcagg tagggatcca ccttctgcgc caggtcgccg ggcaggaagc
     1381 cgaggcgctc gccggcctcc accgccgggc gcaccaggac gatgcgcttg accttgtcgc
     1441 gctcgagcgc gtccaccgcg caggccaccg ccaggtaggt cttgccggtg ccggccggcc
     1501 ccacgccgaa ggtgatgtcg tgcgccagga tctgctcgat gtactgcacc tggcgcggtg
     1561 tgcgcccgtg caggtcggcg cgccgtgtct tcagcggcgt tgccgagtcc tgcgccgcgg
     1621 cggctgcgcc gcgcgacagc tcggtgagcc cgagctggac gtcgtcgacg ctcagctcgg
     1681 cgtccgcctt gccgtagaag tgctccagcg cctgcgccgc gcgcgccgcc tgctgcgccg
     1741 cgccgcgcac cgtgaagcgc gcgccgcggc gcgcgatcga cacgtcgagc gccgattcga
     1801 tctgccgcag gttggcgtcg agcgcgccgc acagccgcgc caggcgccgg ttgtccaccg
     1861 gctcgaggcg gacttcgacg ggcttaggcg gcatcaacca gttcgccgcg cagcgtgtgc
     1921 gcgagcgcgg cggtgatgcg cacctcggcg taagccccgc cgacttccgc cgcggccggg
     1981 aagttgacga tgcggttgtt gccggtgcgg ccggcaagtt ccgccgggtt cttcctcgac
     2041 gggccctcga ccagcacgcg ctgcgtgctg ccgatcatcg ccgcgctgac cgccgccgcc
     2101 tgcgactcga gctgcgcctg caggcgctgc aggcgctcga gcttttcctc gcgcggcgtc
     2161 gggtcgggca gttccgccgc cggcgtgccc gggcgcgggc tgtagaggaa ggagaacgag
     2221 gcgtcgaact ccagctcgcg cgccaggcgc agcgtggcct cgaagtccgc cgcgctctcg
     2281 cccgggaagc cgacgatgaa gtccgacgac aggctgacat ccggccgcgc ctggcgcagc
     2341 cgccgcacga tcgagcggta ctcgagcgcc gtgtagccgc gcttcatggc cgccagcacg
     2401 cggtcggcgc ccgactgcac cggcaggtgc acgtgcggcg cgagcgcgcc gagctcggcg
     2461 tgcgcgtcga cgaggcgctg ggtgaattcg cgcgggtgcg aggtggtata gcggatgcgc
     2521 tccacgccgt cgatctcggc gacgtagcgc agcagttccg cgaaatccgc cggcgcgccg
     2581 ccgatgtcgc cgcgccaggc gttcacgttc tggccgagca gggtgagttc cttcacgccc
     2641 tgcgcggcga gcgcggcggc ctcgcgcacc acgtcctcga aggggcgcga gacctccgcg
     2701 ccgcgcgtgt agggcaccac gcagaagctg cagtacttgc tgcagccttc catgatagtc
     2761 aggaacgcgg cggcaccctc cttgcgaggc gccggcagct ggtcgaactt ctcgatctcc
     2821 gggaagctca cgtccacctg cggcttgccg gtgtcgcggc gtcgcgcaag cagcgcgggc
     2881 aggcggtgca gcgtctgcgg gccgaacacc acgtccacga agggcgcgcg ctcgacgatg
     2941 ccggcgccct cctggctcgc cacgcagccg cccaccgcga tcatcagggc ggggttctcg
     3001 cgcttgaggt gcttcacgcg gcccaggtcg gcgaacacct tctcctgcgc cttttcgcgc
     3061 accgagcagg tgttgaacac gatcaggtcg gcgtcctcgg gcttctcggc cggccgcaga
     3121 ccctcggtct gctcgagaag gcgcgcgatg ctcgccgagt cgacctcgtt catctggcag
     3181 ccgaaggtgc gcaggtagag cttgccggcc atgcgctagg tcgtcagcag gcgcatcgtc
     3241 gaggaaggaa gtggtggcga atcagggact cgaaccccgg acacacggat tatgattccg
     3301 ctgctctaac cagctgagct aattcgccac gcatgtcaaa ttttaatgca gtcagcgact
     3361 taaagccatt cccgccgtcg ctacaatgcc cgcgtgaaga ctgacgtcgc catcgtcggc
     3421 gccgggctcg cgggcctcgc cctcgcccgg gccctgcgcg gcctcgcggt gtcgctggtc
     3481 gcgccggagc gtgccgcgcc gccgccgcag ggctgggacg cgcgcgtgta cgcgatcacg
     3541 ccgggcaacg ccgcgttcct cgccgacctg cgcgtgtggc aggcgctgcc cgccgggcgc
     3601 gtcgcgccgg tgcgcgccat gcgcgtgttc ggcgacgacg gcgccgccgt gctggaattc
     3661 gacgcctacc gcgccggcac ggatgcgctg gcgtggatcg tcgaggacgg ccggctgcgc
     3721 gaggcgctgt gggctgcgct cgctgggcgc gacgacttgc aggtcctcgc cccggcgcaa
     3781 tgcgcccgcc tgcggctcga cgcgcggcgc gcgctcctcg acctggagga cggccgcggg
     3841 atcgaggcgc gcctggtggt cggcgccgac ggcgcgcgct cgcgggtgcg cgagcaggcc
     3901 ggcatcgcgg ccgccgacac gccctacgcg caaagcgcgg tggtggccaa tttcgcctgc
     3961 gcgcgagggc atggcgatac cgcgttgcag tggtttcagg gcggcccggt gctggccctc
     4021 ctgccgctgc ccggcgcgcg cgtgtcgatg gtctggtcgg tggacgaggc cgaggccgcg
     4081 cggctgctcg cgctcgcgcc cgccgcgctg tgccgcgaag tggcgcgggc gagcggccag
     4141 gcgctgggcg aactggaact gatcaccgcg ccgcgcgcgt ttcccctgcg gcgcctcgcg
     4201 gcgcggcgca cggtggcgcc gcgcgtggcg ctggcgggcg acgcggcgca cgtgatccac
     4261 ccgctcgccg gccagggcgc caatctgggc ctgcaggacg cgcgcgcgtt ggccgcggtg
     4321 ctggcgggac gcgagccaca gcgcgacccc ggggaaatcg gcctgctgcg tcgctacgag
     4381 cgcgcgcggg cgcgcgacgt gctggcgatg gacgcgacgg tgcacggcct gtaccggctg
     4441 ttcggcgccg ccgccgggcc ggcggcgggg ttgcgcaacg cggcgttgaa cttggcggac
     4501 cggctcccgg tcctaaagaa cctactcata aggcaggcaa tcacgtgacg agaatcctgg
     4561 tgttcctgct gggcgcgctg ctcgcggcgc ccggcgcatg ggccgacgag gcgaagatcc
     4621 gcagcgtggt ggaggcggcg ctcggcggcg tgcgcatcga gggcgtgcag ccggcgccgg
     4681 tgcccggctt gttcgaagtg cgcttcctgt cgcgcgaagg cccgcagatc gtctacaccg
     4741 atgccgaagc caattacatc ctgcagggaa gcctgttcga cacgcgcagc aagcgcgacc
     4801 tgaccgagga gcgcctgagc aagctctcgg cgatcaagtt cgagtcgctg cccctcgacc
     4861 tggcggtgaa ggtgcggcgc ggcaacggca agcgcgtgct ggcgatgttc tccgacccgt
     4921 actgcccggc ctgccgccag ttcgagcaga cgctggcgca ggtggacgac atcacgatct
     4981 actacttcat gtacccggtg atccggcccg agctcgccga gcactccaag gcggtgtggt
     5041 gctcgccgga ccgcggcaag gcctggctgg cgctcgccgc gcgccccaag gcggcgccgc
     5101 cggcgagcaa cgcgggctgc gacacgccga tcgagcgcgt gctcgagctg ggcaagacgc
     5161 tgcgcgtgaa ttcgaccccg accctgttct tcgccgacgg cgagcgcgtg cgcggcgggc
     5221 tgcgtgcggc cgacctgcgc gccaggctcg accaggcgtc gcgcgccgcc aaggtccgct
     5281 agggcaaagg ccgcggcagc ggcgcgccgc gcggccacag cacccacatc cggccaggct
     5341 ggcgcatgcg cccggcgagc gccccggcct ccgggccggt gccccagaag aagtccgcgc
     5401 gctgcgcgcc gcggatcgcc ccgcccgtgt cctgcgccat gaccaggcgc tccagcggct
     5461 gcggcgagag cgggtaggtg gtggcgagga acaccggcgc gccgagcggc acgtagcgcg
     5521 gatccaccgc gatgctgtag ccggcgtcga gcggcacgcc gagcgcgccg atcgggccgc
     5581 cggccgccgg cagctcgcgg aagaacacgt agctggcgtt gtggttgagc gcctcctgat
     5641 agcgcgcggg gttggcggcc gcccaggcct tgatgccctg catcgaggcc tgctccagcg
     5701 tcagctcgcc gcgctcgacg agcaggcggc cgagcgagcg gtagggatgg ccgttctgct
     5761 cggcgaagcc gacacggatg cgctcgccgg tgtcgagctg gatctgcccc gagccctgga
     5821 tctgcaggaa gaaaagttcc accggatccg ccacccaggc gatcaccggc gcggcgaacg
     5881 cgccgttgcc ggcagcgatc tcgccgcgcg agtaataggg cagcaggcga cgcccgtcca
     5941 gcctgccgcg cagccgcatg ccgcgcagtt ccggatactg cgcggcgagg tccaccacca
     6001 ccaggtcatc cggcaggccg tgcaccggct ggcggtagcg cgcgctgcgc tgccggctgc
     6061 cctcgagcac cggctcgtag tagccggtca ccgtgccctc gacggcgccg tcaggcgccg
     6121 cgatggcgta ggcgctgaag gcgccctcga agaacgcgcg cgctgctgcc tcgtcgccgg
     6181 cgggcaggct gcgcgccgcc tcgcagggcg cctgcatcgc cggctcgagc ggcgcgcgcg
     6241 cgcagccggc gacgaaggcg cgcacgctgg cagcgatcgg ggcaccggcc cagcctggca
     6301 gggaggcgaa atccacggac aggtagcggg cgtgggccgc gggcttgggc ggcggcagct
     6361 gcgcgcagcc ggcagccagt agaatcgcca cccaagctat gtgggtcgtg atcctggaaa
     6421 tgctcctggc gctggcgctg ctggtgctca tcgtctggtg gacctggccg tcgaggcgca
     6481 aggacgactg agattcagtg cagcacgcgc ggcaccgaga gcgtgaactc cgggatcggc
     6541 gcctcgaagc gcgtgccgtc ctcggccacc atctggtagc tgccgcgcat ggtgcccacc
     6601 ggcgtggcga tggaggtgcc gctggtgtac tcgaaactct cgccgggcct gagcacgggc
     6661 tgcgccccca ccacgccgag gccgcgcact tcctgcacca ggccctgcga gtcggtgatg
     6721 atccagtggc ggctgatgag ctgcgcggtg acgttgccgg ggttgcgcat ggtgatggtg
     6781 taggcgaagg cgtagcgccc ggcgctctcg tcggattgtt cggccaggta gcgcgtggcg
     6841 gcgctcactg cgatgtcgta acgcttctcc tgggccatgg gcgcattgta aactcgcgcg
     6901 gccatggcat tccgcatcgc accgagcatc ctgtccgcgg acttcgcgcg cctgggcgaa
     6961 gaagtgcggg ccgtcgaggc cgccggtgcg gacctgctgc acttcgacgt catggacaac
     7021 cactatgttc ccaacctgac cgtggggccg ctggtctgcg cggcgttgaa gccgcacacg
     7081 cgcctgccga tcgacgtgca cctgatggtc aagccggtgg accgcatcat cccggaattc
     7141 gccagggcgg gcgccggcat catcagcttc catcccgagg cctccgagca cgtcgaccgc
     7201 accatcggcc tgatcaagga gcaaggctgc aaggccggcc tcgcgctcaa cccggcgacg
     7261 ccgctcgact gcctggattt caccctggac aagctcgacc tggtgctgct gatgtcggtg
     7321 aatccgggct tcggcggcca gcagttcatc cgcgcggtgc tgcccaagat cgccgaagtg
     7381 cgcaagcgca tcgcgcggct cgggcgccac atctggctgg aagtggacgg cggcgtgaag
     7441 gcggacaaca tcgccgagat cgcgcaggcg ggggccgaca ccttcgtcgc cggctcggcg
     7501 atcttcggcg ccaaggacta tgcggcgacc atccgcgaca tgcgctcgcg cctggtggta
     7561 tagtcgccct cgccgtgact tctctccccc tccgctggcg cagctggcgc ccctggcgcc
     7621 gctgatcact cgcgcgcccc gtgcggcccg gcggcgcgtc tccccaaggc cgtaaggcag
     7681 ttcccaagaa ggtccagcag caggcagcgg agagcatgtc atgaacgagc tggaattcca
     7741 gaagctcgcg cagcagggct atacccggat cccggtactg gcggaagcgc gcgccgacct
     7801 ctacacgccc ctggcggtgt atctcaagct ggccggcggc ccttacagct acctgctcga
     7861 atcggtggtg ggcggggagc gcttcgggcg ctattcgttc atcggcctgc cctgcgccga
     7921 gcgcatcgag gcgcgcggcg gcacggtgcg gcgcctgctg cgcgacgcgc gcggcgccga
     7981 cgtctgcctg gaggaggccg cgggcgaccc cttcgcctac gtgcgcgcct acctcaagcg
     8041 ccaccgcgtg gcgccggccc ccggcgcgct caagtttccc ggcggccttg ccggttactt
     8101 cggctacgag atggtgcgcc acgtcgagcc gcgcgcgctc tcgggcgcca agcccgatgc
     8161 gctgggaacg ccggacatgc tgctgctggt ctcggacgag ctggcggtgg tggacaacgt
     8221 catgggcaag ctccacctgg tcgtgtacgc cgatcccggc cagcccgacg cctggcgcac
     8281 ggcgcaaggg cgcctggaac gcctgcgcgc gcgcctggca gcgccgctcc cggagagcct
     8341 ggtcctcgcg caaccgcgcg cgggcaacgg gccggcgacg ctcgcctgct cgttcaccga
     8401 agccgatttc ctcgccgcgg tcggccgctg caaggaatac gtctacgccg gggactgcat
     8461 gcaggtgcag gtgtcgcagc gcacgtcgcg cgagttccgc gcgccgccga tcgcgctgta
     8521 ccgctcgctg cgcagcctca acccctcgcc ctacatgttc tacttcgatt tcggcgacca
     8581 ccacgtcgtc ggcgcctcgc ccgagatcat ggtgcgcgtg gcgggcgaca agatcacgct
     8641 gcgcccgatc gccggcacgc gagcgcgcgg cgccacgccg caggacgacg cgcgcatcgc
     8701 cgaggagctg ctcgccgatc cgaaggagcg cgccgagcac gtgatgctga tcgacctggg
     8761 caggaacgac gtcggccggg tggccgccgt cggcagcgtc aaggtgaccg agacgatggt
     8821 cgtcgagcgc tactcgcacg tcatgcacat cgtctccaac gtggaggggc gctgcgcgcc
     8881 gggcatggac gcgctcgacg tgttcaaggc gagctttccc gcgggcacgg tcaccggcgc
     8941 gcccaaggtg cgcgccatgc agatcatcga cgagctcgag ccggtgaagc gcggcgtgta
     9001 cagcggcgcg gtgggctacc tcggcttcaa cggcgacatg gacgtggcga tcgccatccg
     9061 caccgcggtg ctgaaggacg gacggctgca cgtgcaggcg gccgccggcg tggtggccga
     9121 ctccgtcccc ctgtcggagt ggcaggagac ccaccacaag gcgcgcgcga tcctcaaggc
     9181 ggcggaggcg gctgcggcgg acggagcgga ggcgccatga tcctggtgct cgacaattac
     9241 gattcgttca cctacaacct ggtgcaatac ctgggcgaac tgggcgccga ggtgaaggtc
     9301 ctgcgcaacg acgaggcgac cgtggacgag gtcgcgcgcc tgcggccgga gaaggtcgtc
     9361 atttcgccgg ggccctgcac gccgaacgag gccgggattt ccctggcgct gatcgagcgg
     9421 ctggcgggct cggtgccgat cctcggcgtg tgcctggggc accaggcgat cggccaggcg
     9481 ttcggcggca aggtggtgcg cgccgcgcag gtgatgcacg gcaaggtctc gcgcgtccgc
     9541 cacgacgggc gcggggtgtt ccagggcctg ccggcggact tcgtcgccac gcgctaccac
     9601 tcgctggcgg tggagcgggg caccctgccc gaggcgctgc aggtcacggc gcaggcggag
     9661 gacggtgaga tcatgggcct gcgccaccgt gcattcgcgg tggaaggcgt gcagttccat
     9721 cccgaggccc tgctcaccga gcacggccac aagatgctgg agaatttcct gaaaggggag
     9781 cggcaatgaa acagatcacg atcgccgagg cgatccagag gacggtggag caccgcgagg
     9841 tgttccacga cgagatgctg cacatcatgc gccagatcat gcgcggcgag ctgacgccgg
     9901 cgcagattgc gggcttcatc accggcctgc gggtgaagaa ggaaaccatc ggcgaaatcg
     9961 ccgccgcggc gcaggtgatg cgcgagttcg ccacgcacgt gaaggtggcc gacgcgcgcc
    10021 atctggtgga cacctgcggc accggcggcg acgcggcgca caccttcaac gtgtccacgg
    10081 cgagcgcctt cgtcgccgcg gccgcggggg cgcgcgtcgc caagcacatg gggcgctcgg
    10141 tgtcttcctc ctcgggcagc gccgaggtgc tggaagcgct gggcgccaac atcgcgctca
    10201 gccccgagca gaccggccag gcgatcgaca agctcggcct cggcttcatg ttcgcgcccc
    10261 tgcaccacgc ggcgatgaag tacgccgcgc cggtgaggaa ggagttgggc gtgcgcaccg
    10321 tgttcaacat cctcgggccc ctcaccaacc cggcgggcgc gccgaaccag gtgatgggcg
    10381 tgttccaccc ggacctggtc ggcatccagg tgcgcgcgct gcagcgcctc ggctcgcagc
    10441 acgtgctggt ggtctacgga ctggaaggcc tggacgagat ctcgatctcc ggcgagaccc
    10501 tggtcggcga gctcgcgaac ggcgagatca gcgagtacac catccatccg cagcagttcg
    10561 gcatcgagct ctacgaccgg cgcgcgatcc aggtgaacac ggtggaggag tccaggcaga
    10621 tgatcctcgc ggtgctggag aaccagcccg ggccggcgct caacatcgtg ctgctcaatg
    10681 ccggcgccgc gctctacgcg gcgggcgtgg cggcctcgat gcaggcgggc atcgacctcg
    10741 cgcgccgcgc catcgccaag ggcgatgcgc gccgcaagct cgacgagttc gtcgcctaca
    10801 cgaagagcgt ggcggcgaag ggctgagcgc gtggcggaca tcctgcagcg catcctcgcc
    10861 agcaagcgcg aggagctgcg cgcggcgcgc gccgccgtgc cgccggcgga aatggcgcgg
    10921 cgggcgcgcg ccgcgccgcc gccgcgcgac ttcgtcgggg ccctgcgcgg gcgcatcgcc
    10981 gccggcggcg cggcggtgat cgccgaggtg aagcgcgcga gtccctcgca ggggctgctg
    11041 cgcgccgact tccgcccggc cgcgatcgcg cagagctacg cgcggggagg ggcggcctgc
    11101 ctgtcggtgt tgaccgaccg cgagtacttc cagggcgcgc cggaacacct cgtgcaggcg
    11161 cgcgccgcct gcgcgctgcc ggtgctgcgc aaggatttca tgctcgatcc ctaccaggtg
    11221 ctcgaagcgc gcgccctggg cgcggattgc atcctgctga tcgcggcggc gctggcgccg
    11281 gcgcagatgc gcgagctgga ggaggcggcg caggagctgg gcatggcggt gctggtcgag
    11341 gtgcacgatg ccgcggagct cgatgcggcg ctcgcgctcg acacgccgct cgtcggcatc
    11401 aacaaccgca acctgcgcag cttcgagacg cggctcgaga ccacgctgga gctgggcgtg
    11461 cgggtgccgg cgccgcgcct ggtgatcgcc gagagcggga tcggcacccg ggcggacgtg
    11521 gaacggcttc ggaacgccgg ggttcgcgca ttcctggtcg gcgaggcctt catgcgggcc
    11581 aaggacccgg gaaatgcgct cgccaagctg tttgaagcag gtgcaactac ttgattaata
    11641 agtcagttag ttggtatttg gcgtatgttg cactttcgca acagcaagcg ccccaatagc
    11701 ggtaaaactc cacggcggcg ttgcttgcgc gccaatgcgc acgcgacaat taaagtaact
    11761 tctcacgtga ggggttggta cgtgccgcgc aagcggcccg gtatttccta caactagttg
    11821 ggagacacac caaatgcaaa agaaactctt gacggtagca gtagccggag cactggcgct
    11881 gcccgctgtc gcgcttgcac aggtgacggt gttcggcacc atcgacggca gcattcgcaa
    11941 ccagagcaag gtaaacatgg ccggcggagc caccggcgcg gtgctgacgg agaactccgg
    12001 tctgcgcacc a
//
LOCUS       DASRIE010000016        10473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1064155, whole genome shotgun sequence.
ACCESSION   DASRIE010000016 DASRIE010000000
VERSION     DASRIE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064155"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /gene="mtgA"
                     /locus_tag="VFB53_00750"
     CDS             complement(<1..605)
                     /gene="mtgA"
                     /locus_tag="VFB53_00750"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZN85287.1"
                     /translation="MRALRVAAWLLAALAAGVLLLQAWYYTHILWWAHEAPASTAFME
                     RRLEQLRARNPKAALRHQWVPYARISPLLKRAVVAAEDAKFIDHDGFDWEAIQKAIQK
                     NERQGRVVAGASTISQQLAKNLFLSGQRSWLRKGQEAIITWMMERTLSKQRILELYLN
                     VAEWGEGVFGAEAAARYHFGVAAASLGPEQAAYLAAVLPSPR"
     gene            complement(593..1393)
                     /gene="aroE"
                     /locus_tag="VFB53_00755"
     CDS             complement(593..1393)
                     /gene="aroE"
                     /locus_tag="VFB53_00755"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN85288.1"
                     /translation="MPDRYAVIGNPVSHSKSPWIHAQFARACGEDMAYEAIEAPPEGF
                     VAAVEKFRAAGGKGLNVTLPFKEQAFRYCAGTSARAAAAQAVNTLVLDREAPYGDNTD
                     GVGLVRDLTVNLQRSLRGAQVLLLGAGGAAQGVVAPLALEGPAGIVIANRTLPRAAAL
                     AARVPGATACGYDALAGRAFDLVVNATSAGLSGELPPLPRGLFRPGALAYDMVYGRET
                     EFLAMARAAGAAASDGAGMLVEQAAESFLLWRGRRPDTRAVLAELCAR"
     gene            complement(1398..2222)
                     /locus_tag="VFB53_00760"
     CDS             complement(1398..2222)
                     /locus_tag="VFB53_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZN85289.1"
                     /translation="MAASLLLHAVLLSVHFKFPNALRWQSANPALDVILVNAKSAQRP
                     GRADALAQANLEGGGDTDQERRARTPLPLIDPRADGRDLAQAQRRVKQLEAQQRKLMT
                     QARRPSASVPADAEQNPSPEQVPAPASGRDLADLSLAAMRLQAQIDRQLEAYQKRPRK
                     KFIGASAVEYRFAQYEEDWRQKVERVGTVNYPAAARGRHYGNLRMTVTLRADGNVESV
                     ELDRSSGLKVLDDAAFRIVRMASPYAAFPAQIRRDTDQLVITRTWFFGKGDKIWTE"
     gene            complement(2349..4232)
                     /locus_tag="VFB53_00765"
     CDS             complement(2349..4232)
                     /locus_tag="VFB53_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZN85290.1"
                     /translation="MHLLYEEEGEFKVGNVLAQAPASFQVESPHGRRSKVKASGVLLS
                     FERPSGAELLAQAQAYAAGLDSEFLWQCCPAGEFGFEDLAREYVGRPPTPVEAAGVLL
                     KLHSAPMYFHRRGRGRYQAASAEILKLALASAEKKRRAQEGIADWAARLARFDCPPEI
                     ARLKDELLYAPDRNKPETKAFEQACAQAGLSGARLLERCGLIPDTHAYHLQRFLHEFF
                     PRGTGFPAYEAPGPVAALPLAEVEAFSLDDQETTEIDDAFSIVRASADEWRVGIHIAA
                     PALGIAPGSPLAAVARERLSTAYMPGFKVTMLPEDVVQRFSLDAGTERPVVSLYLNVA
                     AADFAVRGRHSRLERVRIAANLRHGELEALNAAFESGGSTGHAREEELRCLWRFAQAL
                     EARRGKPAVNAAVADYLFRVHEGRVRIEPRRRGAPLDKLVSELMILANASWGELLAER
                     DVAALYRVQSSGKVRMSVHAEPHEGLGLACYAWMSSPLRRYADLVNQWQLAAALGARK
                     PPFARNSEALLAALRAFEVTYARYDEAQRAMEHYWCLRWLEQEAVREASAVVLRENLV
                     RFESLPLSVRVPSLPVLEPGARVRLELRGIDLLERGLDCVYRETLAPGQGAAPPGDEQ
                     EVR"
     gene            complement(4235..4798)
                     /locus_tag="VFB53_00770"
     CDS             complement(4235..4798)
                     /locus_tag="VFB53_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HZN85291.1"
                     /translation="MIAYLHGFNSSPQSHKAQLTARYLSERGLGAHYACPALPPLAAD
                     AIREIERLRPDESTCYVGSSLGGYYATYLVEKHGGRAVLINPAIDPHAGLRAYLGEQK
                     NLHTGEPYRLTEAHLEEWRRLWVPRITPARYLLLVETGDEVLDYRKAVERYRGAAQVV
                     VQGGDHSLQSFPQHLPRILEFAGLGRA"
     gene            complement(4795..6147)
                     /gene="mpl"
                     /locus_tag="VFB53_00775"
     CDS             complement(4795..6147)
                     /gene="mpl"
                     /locus_tag="VFB53_00775"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZN85292.1"
                     /translation="MAAHLHILGICGTFMGGLAALARQSGLRVTGCDAGVYPPMSEQL
                     AALGIELTEGYGAEQLRLAPDLWVIGNVVTRGNPLMEAILDSGARYVSGPQWLREQVL
                     PGRHLLAVAGTHGKTGTAAMLAWILEHAGRKPGFLIGGIPSNFGVSARLGGSEYFVIE
                     ADEYDTAFFDKRSKLVHYPGKTTILNNLEFDHADIFSDLAAIERQFHHFVRILPRSGK
                     LVVNGRDASLGRVLAMGCWTPVERFGVEEGWDTGRPDAEGGFEVRLQGRGLGRVRWTQ
                     AGEHSRMNALAAIAAARNAGVEPGQAIAALGQFKGVRRRLELRGSAGGVAVYDDFAHH
                     PTAIRTTVEGLRAQQPAGRLLAVLEPRSNTMKLGVMKDQLAASLAAADRVYVYAAGLG
                     WDAASALRALGDKARCIEPLEDLVATIAAEARAGDHVLVMSNGGFGGIHEKLLAALAR
                     "
     gene            6210..6845
                     /locus_tag="VFB53_00780"
     CDS             6210..6845
                     /locus_tag="VFB53_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85293.1"
                     /translation="MSRRNTRQGGMRAEIAAAAARLMAEDGIDDFALAKRKAARQLGA
                     ADTESLPANAEVEEALRAYRALYQADEHPELVLELRRAALEAMRALQQFNPYLTGPVL
                     TGNAGPYAQIELQLYPDSAKDIELFLLERNIAFETSEGRRFAGDRARAVSVFTLDWGG
                     APVRLSVLDPRDERLALKTTAAGRVAERAGIAEVGQLVRKGEGEPPLRTAR"
     gene            7023..7493
                     /gene="aroQ"
                     /locus_tag="VFB53_00785"
     CDS             7023..7493
                     /gene="aroQ"
                     /locus_tag="VFB53_00785"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZN85294.1"
                     /translation="MANSKKSPAILLLNGPNLNLLGSREPQLYGRETLPQIERRLSAR
                     AKAVGVRLECLQTNHEGVAVDRVQQAQRDGVGFIILNPAAFTYSSIALRDALTAVRIP
                     FVEVHLSNIHAREPWRAHSHFTAVAVGSILGLGGRGYDLALEFAIGRLKGGRGA"
     gene            7543..7977
                     /gene="accB"
                     /locus_tag="VFB53_00790"
     CDS             7543..7977
                     /gene="accB"
                     /locus_tag="VFB53_00790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZN85295.1"
                     /translation="MDLRKLKKLIDLVQESGIAELEITEGEEKVKIVKGGEATVTPIA
                     PAPAAPAPSAAPAPGPAEPPAQEGHLLKAPMVGTFYRSASPESKAYVEVGQNVKAGET
                     VCIIEAMKLMNEIEADATGVIKAILVENGQPVEYGQPLFIIA"
     gene            7998..9359
                     /gene="accC"
                     /locus_tag="VFB53_00795"
     CDS             7998..9359
                     /gene="accC"
                     /locus_tag="VFB53_00795"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZN85296.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIRTVVVHSEADTEAKYVKL
                     ADESVCIGPAPARESYLNMAAIISAAEVTDSEAIHPGYGFLSENADFAERVERSGFVF
                     IGPHPENIRLMGDKVSAKQAMEKAGVPCVPGSEGALPEEAKEVVQIARSVGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVNMTRSEAQAAFSNPTVYMERYLENPRHVEIQVLADS
                     HKNALYLGERDCSMQRRHQKIIEEAPAPELTEKQRVRIGERCADACRKIGYRGAGTFE
                     FLYEDGEFFFIEMNTRIQVEHPVTEMITGIDLVQEQIRVAAGEKLRLRQKDIVLRGHA
                     IECRINAEDPYKFTPSPGRITSYHPPGGPGIRVDSHVYAGYTVPPNYDSMLGKVIAYG
                     ATRDQAIRRMRIALSEMAIEGILTNLPLHRELLHDSRFLRGGTSIHYLEQKLAQENKQ
                     KGK"
     gene            9359..10183
                     /gene="prmA"
                     /locus_tag="VFB53_00800"
     CDS             9359..10183
                     /gene="prmA"
                     /locus_tag="VFB53_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZN85297.1"
                     /translation="MPWLALTVELEAAQAEAMSEALLEAGAASVSIEDPEAARSRLRA
                     LLEADADAAALLAAAAARAGCAVPPFRTEAVADQDWVRATQAQFAPLCVAGRLWIVPT
                     WHAAPQDGIVLRLDPGLAFGTGSHPSTRLALAWLARTLAPGASVLDYGCGSGILAIAA
                     AKLGAVRVDAVDVDPLALATTRDNARANGVALRALAPDELPPGDYDVVVANILSQPLI
                     VLEPLLAARTRRGGRIALSGILETQAAEVAAAYGAHFEPRAGDMEEGWALVEGRRR"
     gene            10180..>10473
                     /locus_tag="VFB53_00805"
     CDS             10180..>10473
                     /locus_tag="VFB53_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZN85298.1"
                     /translation="MTLLVTRCPACATAFRAQPGQLSARKGMVRCGKCATVFDGVANL
                     VEEGAGIPGEPSPQLGLFEAGTPGPAAPGRARALDDDEPLPEFLEEAPPRARFS"
BASE COUNT         1558 a         3742 c         3697 g         1476 t
ORIGIN      
        1 cgcgggctcg gcagcaccgc ggcgagatac gccgcctgct cgggcccgag cgaggcggcg
       61 gcgacgccga agtgatagcg cgccgcggct tcggcgccga acacgccctc gccccactcg
      121 gcgacgttga ggtacagctc gagaatgcgc tgcttggaca gcgtgcgctc catcatccac
      181 gtgatgatgg cctcctgccc ctttctcagc cacgagcgct ggccggacag gaacaggttc
      241 ttcgccagct gctggctgat ggtcgaggcc cccgccacca ccctgccctg ccgctcgttt
      301 ttctgtatcg ccttctggat cgcctcccag tcgaagccgt catggtcgat gaacttggcg
      361 tcctcggccg cgaccaccgc gcgcttgagc agcggcgaga tgcgcgcata cggcacccac
      421 tggtggcgca gggcggcttt cgggttcctg gcgcgcagct gctccagccg ccgctccatg
      481 aacgcggtgc tcgccggcgc ctcgtgcgcc caccacagga tgtgggtgta gtaccaggcc
      541 tgcagcagca ggacgcccgc ggcgagcgcg gcgagcagcc aggcggcgac cctcagcgcg
      601 cgcacagctc ggcgagcacg gcgcgcgtgt ccggccgcct gccgcgccag aggaggaacg
      661 attccgcggc ctgctccacc agcatcccgg cgccgtcgct tgccgcggcg cccgcggcgc
      721 gcgccatggc gaggaactcg gtctcgcggc cgtacaccat gtcgtaggcg agcgccccgg
      781 gccggaacag gccgcgcggc aacggcggca gctcgccgct caggcccgcc gacgtggcgt
      841 tgaccaccag gtcgaaggcg cgccccgcga gcgcgtcgta cccgcaggcg gtggcgccag
      901 gaacgcgcgc ggcgagcgcc gcggcgcgcg gcagggtgcg gttggcgatg acgatgccgg
      961 cgggcccctc gagcgccagc ggcgccacta cgccctgggc cgcgccgccc gcccccagca
     1021 gcaggacctg cgctccgcgc aggctgcgct gcaggttgac ggtgaggtcg cgcaccaggc
     1081 cgacgccgtc ggtgttgtcg ccgtagggcg cctcgcggtc gagcaccagc gtgttcaccg
     1141 cctgcgcggc ggcggcgcgc gcgctggtgc cggcgcaata gcgaaacgcc tgctccttga
     1201 agggcagcgt cacgttgagg cccttgccgc cggcggcgcg gaatttctcc accgcggcga
     1261 cgaagccctc cggcggcgcc tcgatggcct cgtacgccat gtcttcgccg caggcgcgcg
     1321 cgaactgcgc gtggatccac ggcgacttcg aatgcgagac gggattgccg attacggcat
     1381 agcggtcggg cacgggctca ttctgtccag atcttgtcgc ccttgccgaa aaaccaggtg
     1441 cgcgtgatca ccagctggtc ggtgtcgcgg cggatctgcg ccgggaaggc ggcgtagggc
     1501 gaggccatgc gcacgatgcg gaacgcggcg tcgtccagaa ccttcagccc cgaggagcgg
     1561 tcgagctcga cggactcgac gttgccgtcc gcgcgcaggg tcaccgtcat gcgcaggttg
     1621 ccgtagtgcc tgccgcgcgc cgccgccggg tagttcaccg tgccgacgcg ctccaccttc
     1681 tggcgccagt cctcctcgta ctgcgcgaag cggtactcga cggcgctcgc gccgatgaac
     1741 ttcttgcgcg ggcgcttctg gtaggcctcc agctggcggt cgatctgcgc ctgcaggcgc
     1801 atcgccgcca gcgacaggtc ggcgaggtcg cgcccggacg ctggcgccgg cacctgctcc
     1861 gggctggggt tctgttcggc atccgcgggc acgctggcgc tggggcggcg cgcctgcgtc
     1921 atgagctttc tctgttgcgc ctcgagctgc ttcacgcggc gctgcgcctg cgcgaggtcg
     1981 cgcccgtcgg cgcgcgggtc gatcaggggc agcggcgtgc gcgcgcggcg ctcctggtcg
     2041 gtgtcgccgc cgccttccag gttggcctgc gccagcgcgt cggcgcgccc cgggcgctgg
     2101 gccgacttgg cgttgaccag gatgacgtcg agcgccgggt tggccgattg ccagcgcagc
     2161 gcattgggaa acttgaagtg caccgacagc aggaccgcgt gcagcaggag cgaagctgcc
     2221 accgacgcgt agaacacgcg ggagcggtgg tcgagcccgg accagaattc ggccgcgccc
     2281 ggaaggcttt tcgctagcgc accctgcaat ggcacctcga ggcgaaattg cgccgccatt
     2341 ctagggcctt agcgcacttc ctgctcgtcg cccggcggcg ccgctccctg cccgggggcc
     2401 agggtttccc ggtagacgca atccaggccg cgctccagga ggtcgatgcc gcgcaattcc
     2461 aggcgcacgc gcgcaccggg ctccaggacg ggcagcgagg gcacgcgcac ggagagcggc
     2521 aggctctcga agcgcaccag gttttcgcgc agcaccacgg cgctcgcctc gcgcaccgcc
     2581 tcctgctcca gccagcgcag gcaccagtag tgctccatgg cgcgctgcgc ctcgtcgtag
     2641 cgcgcgtagg tgacctcgaa ggcgcgcaag gcggcgagca gcgcctcgga attgcgcgcg
     2701 aacggcggct tgcgcgcgcc gagggcggcc gccagctgcc actggttgac gaggtcggcg
     2761 tagcggcgca gcggcgagct catccacgca tagcaggcga ggcccaggcc ctcgtgcggc
     2821 tcggcgtgca cgctcatgcg caccttgccg gaggactgca cgcggtacag cgccgcgacg
     2881 tcgcgctcgg cgagcagctc gccccagctc gcgttggcga ggatcatgag ttcgctcacc
     2941 agcttgtcga gcggcgcgcc gcggcgccgc ggctcgatgc gcaccctgcc ctcgtgcacg
     3001 cggaacaggt agtccgccac ggcggcattc accgccggct tgccgcggcg cgcctccagc
     3061 gcctgcgcga agcgccacag gcagcgcagc tcctcttcgc gcgcgtggcc cgtgctgccg
     3121 ccggattcga aggcggcgtt cagcgcctcg agctcgccgt ggcgcaggtt ggcggcgatg
     3181 cgcacgcgct ccagccgcga atggcggccg cgcaccgcga agtcggccgc cgccacgttc
     3241 aggtacagcg acaccaccgg ccgctcggtc cccgcgtcga gcgagaagcg ctgcaccacg
     3301 tcctcgggca gcatcgtcac cttgaagccc ggcatgtagg cggtggacag gcgctcgcgc
     3361 gcgacggcgg ccagcggcga gccgggggcg atgcccaggg cgggcgcggc gatatggatg
     3421 cccacgcgcc attcgtccgc ggaggcgcgg acgatcgaga acgcgtcgtc gatctcggtg
     3481 gtctcctggt cgtccaggct gaacgcctcg acctcggcga gcggcagcgc cgcgacgggc
     3541 ccgggcgcct cgtacgccgg gaatcccgtg ccgcgcggga agaactcgtg caggaagcgc
     3601 tgcaggtggt aggcgtgggt atccgggatc agcccgcagc gctccagcag gcgcgccccg
     3661 ctcagcccgg cctgcgcgca ggcctgttcg aacgccttgg tctccggctt gttgcgatcc
     3721 ggcgcataca gcagttcgtc cttcaggcgc gcgatctcgg gcgggcagtc gaagcgcgcc
     3781 aggcgcgccg cccagtcggc gattccttcc tgcgcgcggc gcttcttctc cgccgaggcg
     3841 agcgccagct tcaggatttc ggcgctcgcc gcctggtagc ggccgcggcc gcggcggtgg
     3901 aaatacatcg gcgccgagtg cagcttgagc agcacgcccg cggcctccac cggcgtcggc
     3961 gggcggccga catactcgcg cgccaggtcc tcgaagccga actccccggc cgggcagcac
     4021 tgccagagga attcgctgtc gagtcccgcc gcgtacgcct gcgcctgcgc cagcaactcg
     4081 gcaccggagg gccgctcgaa ggacagcagc accccgctcg ccttgacctt cgagcgccgg
     4141 ccgtgcgggc tctcgacctg gaaggaggcc ggcgcctgcg cgagcacgtt gccgaccttg
     4201 aactcgccct cctcctcgta aagcaggtgc atcctcaggc ccggcccagc ccggcgaact
     4261 cgaggatgcg gggcagatgc tgcgggaagg actgcagcga atggtcgccc ccctggacca
     4321 cgacctgcgc cgccccgcga tagcgctcca ccgccttgcg gtaatcgagc acctcgtcgc
     4381 ccgtctccac cagcagcagg tagcgcgcgg gcgtgatgcg cggcacccac agccggcgcc
     4441 attcctcgag gtgcgcttcg gtcagccggt agggctcgcc ggtgtgcagg ttcttttgct
     4501 ctccgaggta ggcgcgcagc ccggcgtgcg ggtcgatcgc cgggttgatc agcaccgcgc
     4561 ggccgccatg cttttccacg aggtaggtcg cgtagtagcc gccgagcgaa gagcccacgt
     4621 agcaggtgct ctcatccgga cgcagccgct cgatctcccg gatcgcgtcg gcggcgagcg
     4681 gcggcagcgc cgggcaggca tagtgcgcgc cgagcccgcg ctcggacagg tagcgggcgg
     4741 tcagctgcgc cttgtgcgac tgcggcgagg aattgaagcc gtggagatag gcgatcatcg
     4801 ggcgagcgcc gccagcagct tttcatggat gccgccgaag ccgccgttgg acatcaccag
     4861 cacatggtcg cccgcgcggg cctcggcggc gatcgtcgcg accaggtcct cgagcggctc
     4921 gatgcaacgc gccttgtcgc ccagcgcgcg cagcgcgctg gccgcatccc agcccaggcc
     4981 ggcggcgtag acgtacaccc ggtcggcggc ggcgaggctc gccgccagct ggtccttcat
     5041 cacgccgagc ttcatcgtgt tggagcgggg ttccaggacc gcgagcaggc gtcccgcggg
     5101 ctgctgcgcg cgcagtcctt cgaccgtggt gcggatggcg gtcgggtggt gagcgaagtc
     5161 gtcatacacc gcgacaccgc ccgccgaccc gcgcagctcc agccgccgcc gcacgccctt
     5221 gaattgcccc agcgccgcga tcgcctggcc cggctcgacc cccgcgttgc gcgcggcggc
     5281 gatcgcggcc agcgcgttca tccggctgtg ttcccccgcc tgggtccagc gcacccggcc
     5341 caggcccctg ccctgcagcc gcacttcgaa gccgccttcg gcgtccggcc tgcccgtatc
     5401 ccagccttcc tcaacgccga agcgctccac cggtgtccag caccccatcg ccaaaactcg
     5461 acccaaggac gcatcgcggc cattcacgac cagttttcct gaacgcggca ggatcctcac
     5521 gaaatgatga aattgacgct ctatggcagc aagatcgctg aaaatgtctg catgatcgaa
     5581 ctccaaattg ttaaggattg tggtcttgcc ggggtaatgc accaatttgg agcgtttgtc
     5641 gaagaacgcc gtgtcgtact cgtcggcctc gatcacgaag tactccgacc cgcctaagcg
     5701 ggcgctgacg ccaaaattgg acggaatgcc gccgatgagg aatcccggct tgcgcccggc
     5761 gtgctcgagg atccaggcga gcatggcggc ggtacccgtc ttcccgtgcg tgccggcgac
     5821 cgcgaggagg tggcggccgg ggagcacctg ctcgcgcagc cattgcgggc ccgagacgta
     5881 gcgcgccccg ctgtcgagga tcgcctccat gagagggttg ccgcgggtga cgacgttgcc
     5941 gatcacccac aggtctgggg ccagccggag ctgctcggcg ccgtagccct cggtgagctc
     6001 gattcccagc gcggcgagct gctcgctcat cggcgggtag acgccggcgt cgcagccggt
     6061 gacgcgcagg ccggactggc gcgcgagcgc cgccagcccg cccatgaagg tgccgcagat
     6121 tcccaggatg tgcaggtgtg cggccatggc gaaagggcgg cgattctacg cgatgggccg
     6181 ggggcccggt gcgctatgat gccccggcaa tgagccgacg aaacacgcgc cagggtggca
     6241 tgcgcgcgga gatcgccgcc gccgcggcgc gcctgatggc ggaagacggg atcgacgact
     6301 tcgcgctcgc caagcgcaag gccgcgcgcc agctgggcgc cgccgatacc gagtcgctgc
     6361 ccgcgaacgc agaggtcgag gaagcgctgc gcgcctaccg agccctgtac caggcggacg
     6421 aacaccccga actggtcctc gaattgcgcc gtgccgcgct ggaggcgatg cgcgccctgc
     6481 agcagttcaa tccgtacctc accggcccgg tgctgaccgg gaacgccggc ccctacgcgc
     6541 agatcgagct gcagctctat ccggacagcg ccaaggacat cgagctgttc ctgctcgagc
     6601 gcaacatcgc gttcgaaacc tccgagggcc gccgctttgc cggcgaccgg gcgcgcgcgg
     6661 tcagcgtctt taccctggac tggggcgggg cgccggtgcg cctgtcggtc ctcgatccgc
     6721 gggacgagcg cctggcgctg aagaccaccg cggccggccg cgtcgccgag cgggctggga
     6781 tcgccgaagt cggccaattg gtccgaaaag gcgagggtga gcctccactt cggacggctc
     6841 gataaggaat ttcaggtcat aacttcgtcg atattgcacc taaaagaact taagtcctga
     6901 ctatcattgt taaaatctga catcggccgt ccgtagcggc ggccctgacg aagggcccaa
     6961 ctcccctagt tagggaggtc cagtcccaat ttcggctata ttgccgtctt cttctgggaa
     7021 aaatggctaa ttccaaaaag tcgccggcaa tactgctgct gaatgggccg aatctgaatc
     7081 tgctcggcag ccgtgaaccg cagctgtacg ggcgcgagac gctgccgcag atcgagcggc
     7141 gcctgtccgc gagggcgaag gccgtcggcg tccggctgga gtgcctgcag accaaccacg
     7201 agggcgtggc ggtcgaccgc gtgcagcagg cgcagcgcga cggcgtcggc ttcatcatcc
     7261 tcaacccggc cgctttcacc tattcgagca tcgccctgcg cgatgccctg acggcggtgc
     7321 gcatcccctt cgtcgaggtg cacctgtcca acatccacgc gcgcgagcct tggcgcgcgc
     7381 attcccactt caccgcggtg gcggtcggct cgatcctcgg gctcggcggc cgcggctacg
     7441 acctcgcgct cgaattcgcc atcgggcgcc tcaagggcgg ccgcggggct tagcccccgc
     7501 cggcgcgagg cgggcgggcc tgcccgcccc aggaggacca ccatggatct gcgaaagctg
     7561 aagaagctca tcgacctcgt gcaggagtcc ggcatcgccg agctcgagat caccgagggc
     7621 gaggagaagg tgaagatcgt caagggcggg gaggcgacgg tgacgccgat cgcgcccgcg
     7681 cctgcggcgc ctgcgccatc cgccgcgccc gcgcccgggc ctgccgagcc cccggcacag
     7741 gagggccacc tcctcaaggc gccgatggtc ggcaccttct accgctcggc ctcgcccgag
     7801 tccaaggcct acgtcgaagt cggccagaac gtgaaggccg gcgagacggt gtgcatcatc
     7861 gaggcgatga agctgatgaa cgagatcgag gccgacgcca ccggcgtgat caaggcgatc
     7921 ctggtggaga acggccagcc ggtggagtac ggccagccgc tgttcatcat cgcgtagacg
     7981 gccgcttcgc ccgcccggtg ttcgaaaaga tcctgatcgc caaccgcggg gaaatcgcgc
     8041 tgcgcatcca gcgcgcctgc cgcgaaatgg gcatccgcac ggtggtggtg cactccgagg
     8101 ccgacaccga ggccaagtac gtgaaactcg ccgacgaatc ggtgtgcatc ggcccggcgc
     8161 cggcgcgcga gagctacctc aacatggcgg cgatcatcag cgccgcggag gtgaccgact
     8221 ccgaagccat ccacccgggc tacggcttcc tgtcggagaa cgcggatttc gccgagcgcg
     8281 tcgagcgctc cggcttcgtg ttcatcggcc cgcacccgga gaacatccgc ctgatgggcg
     8341 acaaggtgag cgccaagcag gcgatggaga aggcgggggt tccctgcgtg cccggctcgg
     8401 aaggcgcgct gcccgaggag gcgaaggagg tggtgcagat cgcgcgctcg gtcggctacc
     8461 cggtgatcat caaggccgcc ggcggcgggg gcgggcgcgg catgcgcgtg gtgcacacgg
     8521 aggctgcgct gctcaacgcg gtcaacatga cgcgcagcga ggcgcaggcg gcgttctcca
     8581 accccaccgt gtacatggag cgctacctgg agaacccgcg ccacgtcgag atccaggtgc
     8641 tcgccgacag ccacaagaac gcgctgtacc tgggcgagcg cgactgctcg atgcagcgcc
     8701 gccaccagaa gatcatcgag gaggcgcccg cccccgaact caccgagaag cagcgcgtgc
     8761 gcatcggcga gcgctgcgcc gacgcctgcc gcaagatcgg ctaccgcggc gcgggcacct
     8821 tcgagttcct gtacgaggac ggcgagttct tcttcatcga gatgaacacg cgcatccagg
     8881 tcgagcaccc ggtgaccgag atgatcaccg gcatcgacct ggtgcaggag cagatccgcg
     8941 tcgccgcggg cgagaagctg cggctgcggc agaaggacat cgtgctgcgc ggccacgcca
     9001 tcgagtgccg catcaatgcc gaggatccgt acaagttcac cccgtcgccg ggacgcatca
     9061 cgtcctacca tccgcccggc gggccgggca tccgcgtcga ctcgcacgtg tacgcgggct
     9121 acaccgtgcc gccgaactac gactccatgc tgggcaaggt gatcgcctac ggcgcgacgc
     9181 gcgaccaggc gatccggcgc atgcgcatcg ccctgtcgga gatggcgatc gagggcatcc
     9241 tgaccaacct gccgctgcac cgcgagctgc tgcacgacag ccgcttcctg cgcggcggca
     9301 cctcgatcca ctacctcgag cagaagctgg cccaggagaa caagcagaag gggaagtagt
     9361 gccctggctg gcgctcaccg tggagctgga ggcggcgcaa gccgaggcca tgagcgaggc
     9421 cctgctggag gcgggcgccg cctcggtgag catcgaggat ccggaggcgg cgcgcagccg
     9481 actgcgcgcg ctgctcgagg cggacgcgga cgccgcggcg ctgctagccg ccgccgcggc
     9541 gcgcgccggt tgcgccgtgc cgccgttccg caccgaggcg gtcgccgacc aggactgggt
     9601 gcgcgccacg caggcgcagt tcgcgccact gtgcgtggcc gggcggctgt ggatcgtccc
     9661 gacctggcac gcggcgccgc aggacggcat cgtgctgcgg ctcgaccccg gcctcgcctt
     9721 cggcaccggc agccatccct ccacgcgcct cgcgctcgcc tggctcgcgc gcacgctcgc
     9781 gcccggcgcc agcgtgctcg attacggctg cggttccggt atattggcga tcgccgccgc
     9841 caagctcggc gccgtgcgcg tcgacgcggt cgacgtggac ccgctggcgc tggcgacgac
     9901 gcgcgacaac gcgcgcgcca acggcgtcgc cctgcgcgcg cttgcgccgg acgagttgcc
     9961 gcccggcgac tacgacgtgg tggtagccaa catcctgtcc cagcccctga tcgtgctcga
    10021 acccctgctc gcggcgcgca cgcgccgcgg gggccgcatc gcgctctccg gcattctgga
    10081 gacgcaggcg gcggaggtcg ccgcggccta cggcgcgcat ttcgagccgc gcgccggcga
    10141 catggaggag ggctgggcgc tggtggaagg gcggcggcga tgaccctgct ggtgacgcgc
    10201 tgcccggcct gcgccacggc gttccgcgcg cagccgggac agctctcggc gcgcaagggc
    10261 atggtccgct gtggcaagtg cgcgacggtg ttcgacggcg tcgccaacct ggtcgaggag
    10321 ggcgccggca tcccggggga gccctcgccg cagctcggcc tgttcgaagc cggcacgccg
    10381 ggcccggcgg cgccgggcag ggcgcgggcg ctggacgacg acgagccgct gcccgagttc
    10441 ctcgaggagg cgccgccgcg cgcgcgcttc agc
//
LOCUS       DASRIE010000017         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_273919, whole genome shotgun sequence.
ACCESSION   DASRIE010000017 DASRIE010000000
VERSION     DASRIE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273919"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFB53_00810"
     CDS             <1..371
                     /locus_tag="VFB53_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN85299.1"
                     /translation="DVLRAEAAKARELDELATWLRSRGATYSADSVTQPAEQLPLSFL
                     PRLAGMKPGEIAVFATPLGASVVQLVHAEDAPLTEQQAAPLIEQFLAGRRRLETAAAE
                     VKRLREVATIEYLGEFKRGN"
     gene            376..1125
                     /gene="epsE"
                     /locus_tag="VFB53_00815"
     CDS             376..1125
                     /gene="epsE"
                     /locus_tag="VFB53_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HZN85300.1"
                     /translation="MKRIAIGIFFLAMAAGAWAQAEKLGIGDAVRITVFQQPDLTTET
                     RISEKGTISMPLIGELKLAGQSQAEAAAQIAGKLKDGQFLKNPQVAVALTTVRSRQVS
                     VLGAVARPGRYPLDDTSSQLSDVIAAAGGVTAAGGDTVMVMRGGKEERVPLLGGKGYA
                     LKGGETINVERAPVFYIHGEVARSGAYRVEPNMTVMQAIAAGGGITPRGSDRRLKLRR
                     VGEDGKLVERDASLRDPVKADDVIFVKEALF"
     gene            1138..2562
                     /locus_tag="VFB53_00820"
     CDS             1138..2562
                     /locus_tag="VFB53_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZN85301.1"
                     /translation="MDLNQFLLALRARRKAFIIAFAATVFTAIAVALIVPKKYVATAT
                     LMADARDEQAMSPVRMSPRERAGYYQTQIDLITSSRIATQVARDLKLAQRPGWREAWE
                     SDTGGQGQIDDWIATQLREKVLVDTSVSNLLLVNYASDNPRFAAEVANAFATAYLNVA
                     LQLRTEPTREAAAWFDEQLKMLRTQVVQGQSRLNAYQKQKGILVEDARMDVESTRLAE
                     LSTQLMAARNATLDAQARYKQATEILESGASPESIPDVLSNAYLTGLKTDLGRIEGRI
                     EQENAVLGPNHPQVLRSAAEAQGLRDKLKSETKKVLAGLGNAVQTSRKREADLQAAIE
                     AQNQRLLALKDWRIEMAGMTREIEAAQRSYDAVLARYMQNKIDSSAKSTNVMLLAPAI
                     EPLKPVHPKLGLISGLALALGLMLATGIVYVLEMLDRRVRSRHDLESRLAVPSLGRLS
                     KWQPTGGRLLPAPMRAARALPQPW"
BASE COUNT          459 a          942 c          919 g          344 t
ORIGIN      
        1 tcgacgtgct gcgcgcggag gccgccaagg cgcgcgagct ggacgagctc gccacctggc
       61 tgcgctcgcg cggcgcgacc tacagcgccg acagcgtgac gcagccggcc gagcagctgc
      121 cgctttcctt tctgccgcgc ctggcgggca tgaagccggg cgagatcgcg gtgttcgcca
      181 cgccgctcgg cgcctcggtg gtgcagctgg tgcacgccga ggacgcgccg ctgaccgagc
      241 agcaggcggc cccgctgatc gagcagttcc tggcggggcg gcggcggctg gaaacggcgg
      301 cggcggaagt gaagcggctg cgcgaagtgg cgacgatcga atacctgggc gaattcaaga
      361 gaggcaactg acatgatgaa acgcatagca atcggcatct tctttctcgc aatggccgcc
      421 ggcgcttggg cgcaggccga gaagctgggc atcggcgacg cagtccgcat caccgtgttc
      481 cagcagcccg atctcaccac cgagacccgc atcagcgaga aaggcaccat ctcgatgccg
      541 ctgatcggcg agctcaagct cgccgggcag tcgcaggccg aggccgcggc gcagatcgcc
      601 ggcaagctga aggacggcca gttcctgaag aacccgcagg tcgcggtggc gctcaccacg
      661 gtgcgcagcc gccaggtctc ggtgctgggc gcggtggcgc gccccgggcg ctacccgctc
      721 gacgacacca gctcgcagct ctccgacgtg atcgccgccg cgggcggcgt caccgcggcg
      781 ggcggcgaca ccgtgatggt gatgcgcggc ggcaaggaag agcgcgtgcc gctgctcggc
      841 ggcaagggct acgccctgaa gggcggcgag accatcaacg tcgagcgcgc gccggtgttc
      901 tacatccacg gcgaggtggc gcgctccggc gcctaccgcg tcgagcccaa catgacggtc
      961 atgcaggcga tcgccgccgg cggcggcatc acgccgcgcg gcagcgaccg ccggctgaag
     1021 ctgcgccgcg tcggcgagga cggcaagctg gtcgaacgag acgccagcct gcgcgatccg
     1081 gtgaaggccg acgacgtcat ctttgtcaaa gaagctctgt tctagggaga tcaagcaatg
     1141 gatctgaacc agttcctcct ggccctgcgc gcacggcgca aggccttcat catcgcgttc
     1201 gccgcgaccg tgttcaccgc gatcgcggtg gcgctgatcg tgcccaagaa gtacgtcgcc
     1261 accgccacgc tgatggcgga tgcgcgcgac gagcaggcca tgagcccggt gcgcatgtcg
     1321 ccgcgcgagc gcgccggcta ttaccagacc cagatcgacc tgatcaccag cagccgcatc
     1381 gccacccagg tggcgcgcga cctcaagctc gcgcagcgcc ccggctggcg cgaggcctgg
     1441 gagagcgaca ccggcggcca gggccagatc gacgactgga tcgccacgca gctgcgcgag
     1501 aaggtgctgg tcgatacctc ggtcagcaac ctgctgctgg tcaactacgc ttccgacaac
     1561 ccgcgcttcg ccgccgaggt ggccaacgcc ttcgccaccg cctacctcaa cgtcgcgctg
     1621 cagctgcgca ccgagccgac gcgcgaggcc gcggcgtggt tcgacgagca gctgaagatg
     1681 ctgcgcacgc aggtggtgca gggacagagc cggctcaacg cctaccagaa gcagaaaggc
     1741 atcctggtcg aggacgcccg catggacgtc gagtcgacgc gcctggccga gctctcgacc
     1801 cagctgatgg ccgcgcgcaa cgcgaccctg gacgcgcaag cccgctacaa gcaggcgacc
     1861 gagatcctgg agagcggcgc ctcgcccgag tcgattcccg acgtgctgtc gaacgcctac
     1921 ctcaccggcc tgaagaccga cctcggccgc atcgaggggc gcatcgagca ggagaacgcg
     1981 gtgctcggcc cgaaccaccc gcaggtgctg cgcagcgccg cggaggccca gggcctgcgc
     2041 gacaagctca agagcgagac caagaaggtg ctggccggcc tgggcaacgc ggtgcagacc
     2101 agccgcaagc gcgaggccga cctgcaggcg gcgatcgagg cgcagaacca gcgcctgctc
     2161 gcgctcaagg actggcgcat cgagatggcg ggcatgaccc gcgagatcga ggccgcgcag
     2221 cgttcctacg acgcggtgct ggcgcgctac atgcagaaca agatcgactc gagcgcgaag
     2281 agcaccaacg tgatgctgct cgcgccggcg atcgagccgc tcaagccggt gcacccgaag
     2341 ctcggcctga tcagcggcct cgcgctcgcg ctcggcctga tgctcgccac cggtatcgtc
     2401 tacgtgctcg aaatgctcga ccgccgcgtg cgctcgcgcc acgacctcga gtcgcgcctc
     2461 gccgtgccgt cgctcggccg gctttccaag tggcagccca cgggcggccg gctgctgccg
     2521 gcgccgatgc gtgctgctcg tgcccttccc cagccctggt aggtgaccaa catgctcgtc
     2581 aagccgactg acaacgtact gccgatggaa gccgcctccg cccgtgccgg cggccgccac
     2641 ctcggcgcca ttctgatgga cgaa
//
LOCUS       DASRIE010000018         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_537462, whole genome shotgun sequence.
ACCESSION   DASRIE010000018 DASRIE010000000
VERSION     DASRIE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537462"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFB53_00825"
     CDS             <1..397
                     /locus_tag="VFB53_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN85302.1"
                     /translation="AVSRAYRELFKPTARRPKPEVIVCTFAIVAESDDEAERLAASID
                     LRRLHMALNIDSPVPTAEEAARHRYSDEERRYVLGQRARAVIGGPEKAKREMNLLVEK
                     YQADELMVLTITGDYESRRRSYELLIGAF"
     gene            complement(377..1597)
                     /locus_tag="VFB53_00830"
     CDS             complement(377..1597)
                     /locus_tag="VFB53_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN85303.1"
                     /translation="MTWAVLTYIALVCVLAGWVHGALGFGFPLVATPLAALAIDMKSA
                     ITLLAPVTLALVVISALRGGSLGELVRRFWFMPVGIALGAWLGTQLLLAAPPEPFILV
                     LALVIVFYLNLDRIGRGESAAVQRLRAPFGFAFAFLAGVFEAIANVAGPILFVYFMLL
                     GAAPSQVVQTLNMCFTVGKGSQVATLAAAGALNAATWAAVAGLTVPSVAALVAGMRVR
                     ERIDAKTYRNWLRKALWVMVVLLILQFSRSALAAEPLFAAISDHREVAALALIDTTDV
                     NARDANGDTPLHRAVETGMRRLTRALLAAGADPQARTRNGETALHLAALHPEPDFADL
                     LLAAKANPRLQNADGESPLHWAALSGHIVVAQRLLARGADARLKTRGGQTARDYASRE
                     GHAEIARLLERADQ"
     gene            complement(1609..>2013)
                     /locus_tag="VFB53_00835"
     CDS             complement(1609..>2013)
                     /locus_tag="VFB53_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZN85304.1"
                     /translation="PDDARAQQLSRQVVSLGELLRARHFVPKLQTKVFVQSHCHQKAL
                     WGAAADLDVLKGAGCEVIAPDTGCCGMSGSFGYKPEHLEASKRIAGLALLPALAKAPD
                     ALVMANGFSCREQIESLAGRPTLHLAEVLAPR"
BASE COUNT          335 a          720 c          692 g          266 t
ORIGIN      
        1 cgcggtgtcg cgcgcctacc gcgagctgtt caagccgacc gcgcgccggc cgaagcccga
       61 ggtgatcgtg tgcaccttcg ccatcgtcgc cgagagcgac gacgaggccg agcgccttgc
      121 cgcctcgatc gacctgcgcc ggctgcacat ggcgctcaac atcgactcgc cggtgccgac
      181 cgcggaagaa gcggcccgcc accgctacag cgacgaggag cggcgctacg tgctcggcca
      241 gcgcgcccgg gcggtgatcg gcgggccgga gaaggcgaag cgcgaaatga atcttctggt
      301 tgaaaaatac caggcggacg agctcatggt gctgaccatc accggcgact acgagtcgcg
      361 ccggcgctcc tacgagctac tgatcggcgc gttctagcag gcgcgcgatt tccgcgtggc
      421 cctcgcggct cgcatagtcg cgcgcggtct ggccgccgcg ggttttcagc cgagcgtcgg
      481 cgccgcgcgc gagcagccgc tgcgccacca cgatgtgccc gctgagcgcc gcccagtgca
      541 ggggcgactc gccgtcggcg ttctgcaggc gcgggttagc ctttgccgcg agcagcaaat
      601 cggcgaagtc cggctcggga tgcagcgcgg cgaggtgcag cgcggtttcg ccgttcctgg
      661 tccgcgcctg cggatcggcg ccggcggcga gcagcgcccg ggtgaggcgc cgcatgccgg
      721 tctcgaccgc ccggtgcagc ggcgtgtcgc cgttcgcgtc gcgcgcgttg acgtcggtcg
      781 tgtcgatgag ggcaagcgca gccacttcac gatgatccga aatggcggca aagagcggct
      841 cggcggcgag cgcgctgcgc gagaactgca ggatgagcag caccaccatc acccacagcg
      901 ccttgcgcag ccagttgcgg taagtctttg cgtcgatgcg ctcgcgcacg cgcatgccgg
      961 cgaccagcgc cgccaccgag ggcacggtca ggcccgccac cgcggcccag gtcgcggcat
     1021 tcagcgcgcc cgcggccgcg agcgtcgcga cctgcgagcc cttgcccacg gtgaagcaca
     1081 tgttcagcgt ctgcaccacc tgcgatggcg ccgcgccgag cagcatgaaa tagacgaaca
     1141 ggatcggccc ggcgacgttg gcgatcgcct cgaacacgcc cgccagaaaa gcgaatgcga
     1201 agccgaacgg cgcgcgcaac cgctgcacgg cggcgctttc gccccggccg atccggtcca
     1261 ggttcaggta gaacacgatc accagcgcga gcaccaggat gaacggctcg ggcggcgcgg
     1321 cgagcagcag ctgggtgccg agccacgcgc cgagcgcgat gccgaccggc atgaaccaga
     1381 agcgccgcac cagctcgccc aggctgccgc cgcgcagcgc ggaaatcacg accagcgcga
     1441 gcgtcaccgg cgcgagcagc gtgatcgccg acttcatgtc gatcgcgagc gccgcgagcg
     1501 gcgtcgccac cagcggaaag ccgaagccga gcgcgccgtg cacccacccc gcgagcacgc
     1561 acaccagcgc aatgtaggtc agcactgccc aggtcaaggt ttgccaactc atcttggcgc
     1621 cagcacctcg gcgaggtgca gcgtcggccg gccggcgagc gactcgatct gctcgcggca
     1681 cgagaagccg ttcgccatca ccagcgcgtc gggcgccttc gccagcgccg gcagcagcgc
     1741 gaggcccgcg atgcgcttcg acgcctcgag gtgctccggc ttgtagccga acgatcccga
     1801 catgccgcag cagccggtgt cgggcgcgat cacttcgcaa ccggcaccct tgagcacgtc
     1861 gagatcggcc gccgcgcccc acagcgcttt ttgatggcag tgggattgca cgaatacctt
     1921 ggtttggagt ttgggaacga aatgccttgc cctcagcaat tccccgaggc tcaccacctg
     1981 ccgggacaac tgctgcgcgc gcgcatcgtc cgg
//
LOCUS       DASRIE010000019         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2362670, whole genome shotgun sequence.
ACCESSION   DASRIE010000019 DASRIE010000000
VERSION     DASRIE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362670"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFB53_00840"
     CDS             complement(<1..813)
                     /locus_tag="VFB53_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85305.1"
                     /translation="MPAAAHGFGQRFDLPLPLWLWVGGAGATLVLTFVVMALFVRDVR
                     LPRMRLCEVPASATTFARWLALLAFLTTVAAGFFGTQDPYRNLAPTMVWVLWWVGFAF
                     VCALVLDVWALANPLAVFPEVPGVVRYPARLGAWPAVALFFAFAWAELVWRDNDVPAY
                     LAAALLAYAAITWAGMLLFGRAAWLEHGDAFSLAFGILGRFAPLERAGRELRLRAPGA
                     GLSGERAPSFSMLVFVLLMLATVTFDGFMETPLYQHLAGIAQDSRLLYELSEL"
     gene            842..1222
                     /locus_tag="VFB53_00845"
     CDS             842..1222
                     /locus_tag="VFB53_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85306.1"
                     /translation="MYFEIKDCAVVLRAARVLGHREAPGDARGELHARGRLRRHRGFE
                     VIAMQMQHDAPVAPPVQLDQVAFGNAQRAAPGGELAPDQLQVEGALGRERQGSRRECR
                     GGGERAAQRWRTSMLPLPSAPSAL"
     gene            complement(1168..>2856)
                     /locus_tag="VFB53_00850"
     CDS             complement(1168..>2856)
                     /locus_tag="VFB53_00850"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HZN85307.1"
                     /translation="LRGSFGDIQLIGLCAYASMRDPSNLALPTTGTSVIDMRVPSQPV
                     WTQTLRTPAMQRAYSALEIQNTTMVAAFKDFGPAGNNWFDIYDVSGNCLEPRHVATTT
                     TASGNHDGWLTPDTNTYYGIPFGGQTIQQNPARIDVHVLDMTDKAKPVHLVNWNRLQL
                     PAEVQARTLATRNFHDVSTNDDGTRLYLALYGGNNALGGNPPDGSGRCANGLLILDSS
                     DVALRRANPQLRYISFLSWCPEQQSADNFVDPDYGDGSTAASHATEFVIHENGRRYIL
                     STDESGAGLDGEWNQHVRQRTFSRLIDISDDANPRVVSTFKPDVNDPDNAIQVAEQQI
                     NGGMLHYIGFDDRNKMRLVFYAGANQGIRVVDFRVPESPREIAYYKAPNVATTRVGEN
                     DFTRPDPRYDQDNCLLYTGWNQGGLRILELTNPEYNACMRRAVNGGGFLAGSRTNIGF
                     DVGRDNGGLGALQGSLSLNDRAAGVRIEIAQVTYLSGVRDQCGAILPAANAVQFNATG
                     TFNGAPASFRVCVQDGGEGGKQAAPDRFFLTCTAGCSYSADGALGNGNIDVRQR"
BASE COUNT          447 a          950 c         1030 g          429 t
ORIGIN      
        1 cagctccgaa agctcgtaca gcaggcggga atcctgcgcg atgcccgcca gatgctgata
       61 gagcggcgtt tccatgaagc cgtcgaaggt gacggtggcg agcatcagga gcacgaacac
      121 gagcatcgag aacgaaggcg cgcgctcgcc ggacaggccg gcgcccggcg cgcgcagccg
      181 cagctcgcgc ccggcacgct ccagcggcgc gaagcgtccg aggatgccaa aggcgaggct
      241 gaacgcatcg ccatgctcga gccaggccgc gcggccgaac agcagcatgc cggcccaggt
      301 gatcgcggca tacgcgagca gcgcagccgc caggtatgcg ggcacatcgt tgtcgcgcca
      361 caccagctcg gcccaggcga acgcgaagaa cagcgccacc gccggccacg cgccgagccg
      421 cgccggatag cgcaccacgc ccggtacttc cggaaaaacg gcgagcggat tcgcgagcgc
      481 ccacacgtcg aggaccagcg cgcacacgaa agcgaatccc acccaccaca gcacccacac
      541 catggtgggc gcgaggtttc gataggggtc ctgcgtgccg aagaagcccg cggccacggt
      601 cgtcaggaag gcgagcagcg ccagccacct cgcaaaagtg gttgccgacg ccggcacttc
      661 gcacagccgc atccgcggca ggcgcacgtc gcgcacgaac agcgccatca ccacgaacgt
      721 gagcaccagc gtggcgccgg cgccgcccac ccagagccac agcggcagcg gcaggtcgaa
      781 gcgctggccg aacccgtgcg ccgccgctgg catcgcggcg cacgccagtg acaggctaag
      841 gatgtacttc gagataaagg actgcgcggt ggtgctccgc gccgcgcgtg tgctcggaca
      901 ccgggaagcg cccggcgatg ctcgcggtga acttcatgcg cgcgggcgcc tccggcgtca
      961 ccgtggcttc gaggtcatag ccatgcagat gcagcacgat gcgccggtcg ctcctccagt
     1021 gcaactcgac caggtcgcct ttggcaacgc gcagcgtgct gctcccggcg gcgagcttgc
     1081 ccccgaccag ctgcaggtcg aaggcgcgct cggccgcgag cgccagggta gccgccgcga
     1141 gtgccgcggc ggcggcgagc gcgcggctca gcgctggcgc acgtcgatgt tgccgttgcc
     1201 gagcgccccg tccgcgctgt agctgcagcc cgcggtgcag gtgaggaaga agcggtcggg
     1261 cgccgcctgc ttcccgcctt ctccgccatc ctgcacgcat acgcggaagc tcgcgggcgc
     1321 gccgttgaac gtgcccgtgg cgttgaactg caccgcgttg gccgcgggca ggatcgcgcc
     1381 gcactggtcg cgcacgccgc tcaggtaagt cacctgggcg atttcgatcc gcacgccggc
     1441 cgcgcgatcg ttgagcgaga gcgagccttg cagcgcgccc agcccgccgt tgtcgcgtcc
     1501 gacgtcgaag ccgatgttgg tgcggctgcc ggcaaggaag ccgccgccgt tcaccgcgcg
     1561 ccgcatgcac gcgttgtatt ccgggttggt gagctcgagg atgcgcaacc cgccctggtt
     1621 ccaccccgtg tacagcaggc agttgtcctg gtcgtagcgc gggtcgggcc gggtgaagtc
     1681 gttttcaccc acgcgcgtgg tcgccacgtt gggcgccttg tagtaggcga tctcccgcgg
     1741 gctctcgggc acgcggaagt cgaccacgcg gatgccctgg ttcgcgccgg cgtagaacac
     1801 cagccgcatc ttgttgcggt cgtcgaagcc gatgtaatgc agcatgccgc cgttgatctg
     1861 ctgctccgcg acctggatcg cgttgtccgg gtcgttgacg tccggcttga acgtgctgac
     1921 cacgcgcggg ttggcgtcgt ccgagatgtc gatcaggcgg ctgaaggtgc gctggcgcac
     1981 gtgctggttc cactcgccgt cgagcccggc gccggactcg tcggtcgaga ggatgtagcg
     2041 ccggccgttc tcatggatca cgaactcggt ggcgtgcgac gccgcggtcg agccgtcgcc
     2101 gtagtcggga tcgacgaagt tatcggcgct ctgctgctcg gggcaccagg agaggaagct
     2161 gatgtagcgc agctgcgggt tcgcgcggcg cagggcgaca tcggacgaat ccaggatcag
     2221 caggccgttg gcgcagcgcc cggagccgtc gggcgggttg ccgcccagcg cgttgttgcc
     2281 gccgtagagc gcgaggtaga gccgggtgcc atcgtcgttg gtgctcacgt cgtggaagtt
     2341 gcgcgtggcg agcgtgcgcg cctggacttc ggccggcagc tgcagccggt tccagttcac
     2401 gagatgcacc ggcttcgcct tgtcggtcat gtcgagcacg tgcacgtcga tccgcgccgg
     2461 gttctgctgg atggtctgcc cgccgaaggg aatgccgtag tacgtgttgg tgtcgggcgt
     2521 gagccagccg tcgtggttgc ccgaggccgt ggtggtggtg gcgacatggc gcggctctag
     2581 gcagttgccc gagacgtcgt agatgtcgaa ccagttgttg ccggcggggc cgaaatcctt
     2641 gaacgcggcg accatcgtgg tgttctggat ctcgagcgcc gagtaggcgc gctgcatcgc
     2701 cggcgtacgc agcgtctggg tccagaccgg ctgcgatggc acgcgcatgt cgatcaccga
     2761 ggtgccggtg gtgggcaggg cgaggttgga cgggtcgcgc atgctggcgt aggcgcacag
     2821 cccgatcagc tggatgtcgc cgaagctgcc gcgcag
//
LOCUS       DASRIE010000020         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3670053, whole genome shotgun sequence.
ACCESSION   DASRIE010000020 DASRIE010000000
VERSION     DASRIE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670053"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFB53_00855"
     CDS             complement(<1..1070)
                     /locus_tag="VFB53_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZN85308.1"
                     /translation="MNGPLAIVDVETTGSDPARDRITEIALLEVNGMALTGQWSTLIN
                     PGGTIPGAIQALTGISQEMVERAPRFEQIAAELYQRLAGRVFVAHNARFDYGFLRREF
                     DRAGLKYLAKTLCTVRLSRRLYSGESRHDLDSLIERHGLECETRHRAAPDAGVLWQFL
                     RVAAQDHGAEVLEVAARQVARQPTLPPHLERGAIDAIPEAPGVYLFYDEGAAPLYIGK
                     SRSMRSRVLQHFIATSSWTPRVRRIEWQRTAGELGALLREAALVKELDPVYNRQLRRP
                     GKLCGFAFDGRRLRLARGEEIDAETLPYVYGLWRSRPAAMQALRDAADQHRLCLQTLG
                     FDVKKQGACLRHQIGRCAGVCAG"
     gene            1124..1456
                     /locus_tag="VFB53_00860"
     CDS             1124..1456
                     /locus_tag="VFB53_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85309.1"
                     /translation="MRPRVALLFVPTLAAGLAACTPMQWVKPDASAEQIRMDEQACRQ
                     AAWREAQFQAFLYQHQFQPMVVAPGQVIWPSGAMVDPYGHQLLYENRLADFCMESKGY
                     QLVPVPKP"
     gene            complement(1446..2255)
                     /locus_tag="VFB53_00865"
     CDS             complement(1446..2255)
                     /locus_tag="VFB53_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HZN85310.1"
                     /translation="MKVYGVDFTCAPRRAKAITVAAGVLRNRTLRIEEIENLQSFAEF
                     EAFLARPGPWIGGFDFPFSLPLELVRDLGWPKPWRELVAHCAAMTRFELRAALDAYRN
                     SRQPGSKYAHRATDLPAGSSSPMKLVNPPVALMFHEGARRLAAAEIDLPHLLPRTSSR
                     IALEAYPGLLARKQLGVRASYKSDTRSEHTPARKAARRRIVRALKAGEPLGIRLDAPR
                     AIEKRALEDGSGDTLDAVICALQAAWGWQRRRSNYGLPAHAPAGEGWIVTA"
     gene            complement(2252..>2512)
                     /locus_tag="VFB53_00870"
     CDS             complement(2252..>2512)
                     /locus_tag="VFB53_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN85311.1"
                     /translation="LESWLGRSSMVAERVMEYGSYHAIVACAAAGSGIAVVPRSVLRA
                     SGMEAQLAVHALPPKIANARTLLVWRRGHRSPALEALRKELR"
BASE COUNT          361 a          882 c          885 g          384 t
ORIGIN      
        1 cccgcgcata cgcccgcgca gcgcccgatc tggtggcgca ggcaggcgcc ctgctttttc
       61 acgtcgaagc ccagcgtttg caggcacagg cggtgctggt ccgccgcgtc gcgcagcgcc
      121 tgcattgcgg caggacgcga gcgccacagg ccgtacacgt acggcagcgt ctcggcgtcg
      181 atctcctcgc cgcgcgccag tcgcaggcgg cggccgtcga aagcgaagcc gcagagcttc
      241 cccgggcggc gcagctggcg gttgtagacc ggatcgagtt ccttcaccag cgcggcctcg
      301 cgcagcagcg cgccgagctc gcccgcggtg cgctgccact cgatgcggcg cacgcgcggc
      361 gtccagctcg aggtggcgat gaagtgctgc agcacgcgcg agcgcatcga tctgctcttg
      421 ccgatgtaga gcggcgccgc gccttcgtcg tagaacaggt agaccccggg cgcctccgga
      481 atcgcgtcga tcgcgccgcg ctcgaggtgc ggcggcagcg tgggctggcg cgcgacctgg
      541 cgcgctgcga cctcgagcac ctccgcgccg tggtcttgcg cggcgacgcg cagaaactgc
      601 cacagcacgc cggcatccgg cgcggcgcgg tggcgcgtct cgcattccag cccatgccgc
      661 tcgatcaggc tgtcgaggtc gtgccgcgac tcaccggaat agagccggcg cgacaggcgc
      721 acggtgcaga gcgtcttcgc caagtacttc agcccggcgc ggtcgaactc gcggcgcagg
      781 aagccatagt cgaagcgtgc gttgtgcgcg acgaacaccc ggccggcgag gcgctggtag
      841 agctcggccg cgatctgctc gaagcgcggc gcgcgctcga ccatctcctg gctgatgccg
      901 gtgagcgcct ggatggcgcc ggggatcgtg ccgccgggat tgatcagggt ggaccattgg
      961 ccggtgagcg ccatcccatt tacctccagc agcgcgatct cggtgatgcg gtcgcgcgcc
     1021 gggtcggagc cggtggtttc gacgtcgacg atcgcgagcg ggccgttcat ggacgtttat
     1081 acagtaacgc actttccgcg cgcgcggctg tctaaaattc ggaatgcgcc cgcgcgtcgc
     1141 gcttctattc gtccccacgc tcgccgccgg tctcgccgcg tgcacgccga tgcagtgggt
     1201 gaagcccgac gcgtccgccg agcagattcg catggacgag caggcctgcc gccaggcggc
     1261 gtggcgcgag gcccagttcc aggccttcct ctatcagcac cagttccagc cgatggtggt
     1321 cgcgccgggc caggtgatct ggccgagcgg cgcgatggtc gatccctacg gccatcagct
     1381 gctgtacgaa aaccgcctgg cggatttctg catggagtcg aagggctacc agctcgttcc
     1441 ggtgcctaag ccgtaacgat ccagccctcg cccgccgggg cgtgcgccgg cagcccgtag
     1501 ttcgaacgcc tccgctgcca gccccacgcg gcctgcagcg cgcagatcac cgcgtcgagc
     1561 gtgtccccgg agccgtcttc cagcgcgcgc ttctcgatcg cgcgcggcgc gtcgaggcgg
     1621 atgccgagcg gttcccccgc cttcaacgcc cgcacgatcc tgcgccgcgc cgccttgcgt
     1681 gccggcgtgt gctcgctgcg cgtgtcgctc ttgtaggacg cgcgcacgcc gagctgcttg
     1741 cgggcaagca ggccgggata ggcctccagc gctatcctgg aagaggttct gggtagaagg
     1801 tgcggcaggt cgatctcggc ggcggcgagc cggcgtgcgc cctcgtggaa catgagcgcg
     1861 accggcggat tcaccagctt catcgggctg gacgagcccg ccggcagatc ggtcgcgcgg
     1921 tgcgcgtact tgctgccggg ctggcgtgag ttgcgatagg catccagagc ggcgcgcagc
     1981 tcgaatcgcg tcatcgccgc gcagtgcgcg accagctcgc gccagggctt cggccagccc
     2041 aggtcgcgca ccagctcgag cggcaggctg aaggggaagt cgaagccgcc gatccacggg
     2101 ccggggcgcg cgaggaaggc ttcgaactcg gcgaagctct ggaggttttc gatttcttca
     2161 atgcgcagcg tcctgttcct caaaacgcct gccgccacgg tgatcgcctt cgcccggcgc
     2221 ggagcacagg tgaagtcgac gccgtagacc ttcaccgcag ctctttgcgc agggcctcga
     2281 gcgcgggcga ccggtggcca cgccgccaga ccagcagggt gcgcgcgttg gcgatctttg
     2341 gcggcagggc gtgcaccgca agctgcgctt ccatgccgct cgcgcgcagc acggagcgcg
     2401 gcaccacggc gatgccgctg ccggccgcgg cgcaggcaac gatcgcgtga tacgagccgt
     2461 actccatgac gcgctccgcc accatgctcg agcgccccag ccaggattcg ag
//
LOCUS       DASRIE010000021         7323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_20604, whole genome shotgun sequence.
ACCESSION   DASRIE010000021 DASRIE010000000
VERSION     DASRIE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20604"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1880)
                     /locus_tag="VFB53_00875"
     CDS             complement(33..1880)
                     /locus_tag="VFB53_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZN85312.1"
                     /translation="MSWAGKILRVNLTDGTIASEPLNMTWARQYLGSRGLATKYFVEE
                     VDATVDPLSAANKIIWATGPLTGTMASTGGRYTVITKGPLTGAIACSNSGGHWGAELK
                     MAGWDMVVFEGRSPKPVYLYIQDDVVELRDASGLWGKSVWETEETLKKTLQDPLVRVS
                     SIGLAGENKVLYAAIVNDLHRAAGRSGVGAVMGSKNLKAIAVRGTKGVGNVRDPKAFM
                     AAVNAGKKVLRDNAVTGQGLPTYGTQVLMNVINEVGALPTRNHIDSQYEGAKDISAEA
                     MATPRKSDGKKHLVTNQACFGCTIACGRISKMDEGHFTVENKPQYWGASGGLEYEAAW
                     ALGANSGVNDLEALQYANMVCNEQGMDPISFGATVSAVMELYQLGVLTKEQIGIEAPF
                     GSAAALAKLAELTATGEGFGKDMGLGSKRLCEKYGHPELSMSVKGQEFPAYDGRGIQG
                     MGLAYATSNRGACHLRGYTVASEILGIPVKTDPLVSEGKPELVKAFQDVTAAFDSAGI
                     CIFTSFAWSLADVAPQVAAACGEEFTVENMTTIGERIWNMERDFNNRAGLTSKDDSLP
                     PRLLNEAAKTGPAKGLTNKLPEMLPKYYAARGWNADGTLTASTRSRLGL"
     gene            complement(1905..2381)
                     /locus_tag="VFB53_00880"
     CDS             complement(1905..2381)
                     /locus_tag="VFB53_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZN85313.1"
                     /translation="MQKTLHINADKCTGCLQCEMACSYDNYGVFATSKSRIKVFDFHH
                     TGRKVPYTCTQCDEAWCLHSCPVEAITIDTLTGAKVVDEGTCVGCKVCTISCPFGTIN
                     YVQETGKVQKCDLCGGEPACAEACPTAAITYVDADWTGLDRMRAWADKLGNQPHAA"
     gene            complement(2661..3656)
                     /locus_tag="VFB53_00885"
     CDS             complement(2661..3656)
                     /locus_tag="VFB53_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN85314.1"
                     /translation="MDSLDLQVLAQARDWFAAGRRVWLVTVIETWGSAPRPPGALLAL
                     RDDGLVVGSVSGGCVEDDLIDRVRHGERVGVPSLVTYGVTKEEAARFGLPCGGNLRLV
                     QEPLLDPGWIGEVLARTARHELVARTLELATGAVALEPAQRDESFAFDGRRLRAVYGP
                     RWRLLVIGAGQLSRVLAQMALPLDFEVTCCDPREEYHLTWDVPGTDFRKDMPDDVAAA
                     MQLDPHSAVVALTHDPKLDDMALLEALKSPAFYVGALGSRGNTAKRKERLAMFDLTAQ
                     EIDRLHGPVGLDLGGKTPAEIAVSIVAEIIAVRNGVELVQKKQGSSLPQRHAAAG"
     gene            complement(3813..4526)
                     /locus_tag="VFB53_00890"
     CDS             complement(3813..4526)
                     /locus_tag="VFB53_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85315.1"
                     /translation="MRLGDRMTPLLELRGATVRFGPKVALNAVDLAVARGERIALVGA
                     NGSGKSTLLRALHGLVPLAGGERRMAGAPRIAMVFQRPFMLRMTVRANVELGLMLQRV
                     PRAERRERAMHALERVGLASEAGRIGRVLSGGQQQRAALARAWALRPEVLLLDEPTAS
                     LDPSAKREVETLVGEFAAQGITVLISSHNLGQVKRLASRVVYLEHGRLLADVPTARFF
                     DGPLPEEAMRFLRGELPWD"
     gene            complement(4585..5307)
                     /locus_tag="VFB53_00895"
     CDS             complement(4585..5307)
                     /locus_tag="VFB53_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85316.1"
                     /translation="MNPFLDSLATAARLVADRDPQLLHIVGLSLSVSGIACLVATALG
                     LLAGAMIAVWRFPGRSGVIVVLNTLLALPSVVVGLIVYLLLSRAGPLGAWGILFTPAA
                     MVIAQSLLVTPIVAALARQTIEDAMRDHGEQLRSLGATRLECALIIAWDERLAILTLA
                     LMAFGRAVSEVGAVMMVGGNIAGVTRVMTTAIALETSKGDLPVALALGFVLLGVVLVL
                     NVVIGALRGWRIRGDREAGVLP"
     gene            complement(5353..6582)
                     /gene="senA"
                     /locus_tag="VFB53_00900"
     CDS             complement(5353..6582)
                     /gene="senA"
                     /locus_tag="VFB53_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HZN85317.1"
                     /translation="MDLESADVHSPLMRRAGRDLLSLALMDARNRTLRWLAALEDALG
                     APPWSLPPQPDLDPPLWRIGHVAWFQERWVARNVHRQRGAEADPTAPRLPSVLPQADR
                     WYDPAQSAREQRWSMALPDAAATRQYLADTMELTLELLESAEETDPALYAYRLALFHE
                     DLQAEALAETAQGLGIAIASRHALVGDPLATPPREPLFFPATRWMLGSAPGGFVFDNE
                     KWAHEVAVPEFEIDAHPVTWSQYGEFVEDGGYDEPRWWSPHGLAWLQREGRRVPRYVD
                     QLRGGVLQQRFGRLARVPGGQPAMHVSWHEADAWCRWAGRRLPAEVEWEAAAQSYSRG
                     FAWGGVREWTASTFRPYPGFVPDPWADPSRAHFGQAKSQRGASFATPARLRHTRLRRW
                     AMAEDDRGFCGFRSCAT"
     gene            6643..>7323
                     /locus_tag="VFB53_00905"
     CDS             6643..>7323
                     /locus_tag="VFB53_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85318.1"
                     /translation="MPLLIPFASTLSEASRHALRDLALPNLAKLLAHSRPVARWGGDE
                     YTLTPPHERALAEAQGWSGDDGALPWAALHAAQDGIAPGDDAWGELTPAHWHVGRDHI
                     SLADPLALGLAAAESRTVFDAVRPLFESEGWRMAWGAPTRWYAAHESLHGLPCASLDR
                     VIGRNIDAWLTDAPGRHLWPRLHSEAQMLLHGHPINERRESQGLSSVNSVWLSGCGAP
                     RPASGEAPC"
BASE COUNT         1015 a         2704 c         2584 g         1020 t
ORIGIN      
        1 aaccgccttc tctcaaaacc agcgcaacag catcaaaggc cgagcctgga tcgcgtcgac
       61 gcggtcagcg tgccgtcggc gttccagccg cgcgccgcgt agtacttggg cagcatctcg
      121 ggcagcttgt tggtcaggcc cttggccggg ccggtcttgg cggcctcgtt gagcaaccgc
      181 ggcggcagcg agtcgtcctt ggacgtcaag ccggccctgt tgttgaagtc gcgctccatg
      241 ttccagatgc gctcgccgat ggtcgtcatg ttctccacgg tgaactcctc gccgcacgcg
      301 gccgccacct gcggcgcgac gtcggcgagc gaccaggcga agctggtgaa gatgcagatg
      361 ccggccgagt cgaaggcggc cgtgacgtcc tggaaggcct tcaccagctc gggcttgccc
      421 tcggagacga gcgggtcggt cttcaccggg atgccgagga tctcggaggc gaccgtgtac
      481 ccgcgcaggt ggcacgcgcc gcggttgctg gtggcatagg ccaggcccat gccctggatg
      541 ccgcgcccgt cgtaggccgg gaactcctgg cccttgacgc tcatggagag ctcggggtgg
      601 ccgtacttct cgcacaggcg cttggagccc aggcccatgt ccttgccgaa gccctcgccg
      661 gtggcggtga gctcggccag cttcgccagc gccgcggccg agccgaacgg cgcctcgatg
      721 ccgatctgct ccttggtgag cacgccgagc tggtagagct ccatcaccgc gctcacggtg
      781 gcgccgaacg agatcgggtc catgccctgc tcgttgcaca ccatgttggc gtactgcagc
      841 gcctcgagat cgttcacgcc gctgttggcg cccagcgccc acgccgcctc gtactccagg
      901 ccgcccgagg cgccccagta ctgcggcttg ttctccaccg tgaagtggcc ttcgtccatc
      961 ttgctgatgc gcccgcacgc gatggtgcag ccgaagcacg cctggttggt caccaggtgc
     1021 ttcttgccgt cgctcttgcg cggcgtggcc atcgcctcgg cggagatgtc cttcgcgcct
     1081 tcgtactggc tgtcgatgtg gttgcgcgtg ggcagcgcgc ccacctcgtt gatgacgttc
     1141 atcagcacct gcgtgccgta ggtgggcagg ccctgccccg tcaccgcgtt gtcgcgcagc
     1201 accttcttgc cggcgttcac cgcggccatg aaggccttcg ggtcgcgcac gttgcccacg
     1261 cccttggtgc cgcgcacggc gatcgccttc aggttcttcg agcccatcac cgcgcccacg
     1321 cccgagcggc cggccgcgcg gtgcaggtcg ttcacgatgg cggcgtagag caccttgttc
     1381 tcgccggcca ggccgatgct cgacacgcgc accagcggat cctgcagcgt cttcttcagc
     1441 gtctcctcgg tctcccagac cgatttcccc cacaggcccg acgcgtcgcg cagctcgacc
     1501 acgtcgtcct ggatgtacag gtagaccggc ttcggggacc ggccctcgaa gacgaccatg
     1561 tcccagcccg ccatcttgag ctcggcgccc cagtggccgc ccgagttcga gcaggcgatg
     1621 gcgccggtga gggggccctt ggtgatgacc gtgtagcggc cgccggtgga ggccatcgtg
     1681 ccggtgagcg gcccggtggc ccagatgatc ttgttcgcgg cggacagcgg gtcgacggtg
     1741 gcgtcgactt cctccacgaa atacttggtg gcgaggccgc gcgagccgag gtactgccga
     1801 gcccacgtca tgttgagcgg ctcggaggcg atggtgccgt cggtgaggtt gacgcgaagg
     1861 atctttccag cccaggacat ttcagttctc ctcgtagcgg cggatcaggc ggcgtgcggc
     1921 tggttgccga gcttgtcggc ccaggcgcgc atgcggtcga ggccggtcca gtcggcgtcg
     1981 acgtaggtga tggcggcggt ggggcacgcc tcggcgcagg caggctcgcc gccgcacagg
     2041 tcgcacttct gcaccttgcc ggtttcctgc acgtagttga tggtgccgaa cgggcagctg
     2101 atggtgcaca ccttgcagcc cacgcacgtg ccttcgtcga ccaccttggc gccggtgagc
     2161 gtgtcgatgg tgatggcctc caccgggcac gaatgcaggc accaggcctc gtcgcactgc
     2221 gtgcaggtgt agggcacctt gcggccggtg tgatggaagt cgaacacctt gatgcgcgac
     2281 ttgctggtgg cgaagacgcc gtagttgtcg tacgaacacg ccatctcgca ctgcaggcag
     2341 cccgtgcact tgtcggcgtt gatgtgaagt gtcttctgca tggccacggc ctccttcgga
     2401 tgaaatgcgc gccgcgacgc ggggcttggg ggcgcgtcgg aggcgatgat gttctgcgcc
     2461 gggaaacgag caaaggtggg ctttaccctt aggcgcggca ggggaaaacg atcgcctgtt
     2521 ccacgctgaa acagcggacg gcgcgggccc gtcccgaccc ggatcgccga tgcatcggct
     2581 cgcgagggaa cgccgggccg ccccaggctt cctcgacccc cgcgggggcc ccggctgcgc
     2641 gcagcccggc cctggggccc tcagccggcg gcggcgtgcc gttgcggcag cgaggacccc
     2701 tgcttcttct gcaccagctc cacgccgttg cgcaccgcga tgatctccgc cacgatcgac
     2761 actgcgatct cggccggcgt cttgccgccc aggtccaggc ccaccggccc gtgcaggcgg
     2821 tcgatctcct gcgccgtgag gtcgaacatc gcgaggcgct ccttgcgctt ggcggtgttg
     2881 ccgcgcgagc cgagcgcgcc cacgtagaac gcgggggact tcaaggcctc gagcaacgcc
     2941 atgtcgtcga gcttggggtc gtgggtgagc gccaccacgg cgctgtgcgg atcgagctgc
     3001 atggccgcgg ccacgtcgtc gggcatgtcc ttgcggaagt cggtgcccgg cacgtcccag
     3061 gtgaggtggt actcctcgcg cgggtcgcag caggtgacct cgaagtccag cggcagcgcc
     3121 atctgcgcca gcacgcgcga gagctggccg gcgccgatga cgagcagccg ccagcgcggg
     3181 ccgtagaccg cgcgcaggcg ccggccgtcg aaggcgaacg attcgtcgcg ctgcgcgggc
     3241 tccagcgcga ccgcgccggt ggcgagctcc agcgtgcgcg ccaccagctc gtggcgcgcg
     3301 gtgcgcgcga gcacctcccc gatccatccc gggtcgagca gcggctcctg caccaggcgc
     3361 aggttgccgc cgcacggcag cccgaagcgg gcggcctcct ccttggtcac gccgtaggtg
     3421 acgagcgagg gcacgcccac gcgctcgccg tggcgcacgc ggtcgatcag gtcgtcctcg
     3481 acgcagccgc cggacaccga cccgaccacc agcccgtcgt cgcgcagcgc gagcagcgcg
     3541 ccgggcggcc gcggggccga gccccaggtc tcgatgaccg tcaccagcca tacgcggcgg
     3601 ccggcggcga accagtcgcg cgcctgcgcc aggacttgga gatcgaggct gtccacgggt
     3661 cgggctccgg aaggaaaagt gcggctcgac gagcctagcg cggaccggct cccttcggct
     3721 gtggcggagg gccggggtgg ggccggcgcg cgcgggggcg gctcgtccgg cccggtcacc
     3781 ctaccctgcc cggtagggcg agggaaatac ggtcaatccc atggcagctc gccgcgcagg
     3841 aaccgcatcg cctcctccgg cagcggcccg tcgaagaagc gcgccgtcgg cacgtcggcc
     3901 agcagccggc cgtgctccag gtacaccacc cggctcgcga gacgcttcac ctgccccagg
     3961 ttgtgcgagc tgatgagcac ggtgatcccc tgcgccgcga actccccgac cagcgtctcc
     4021 acctcgcgct tggcgctcgg gtccaggctc gcggtgggct cgtcgagcag cagcacctcc
     4081 ggccgcaggg cccaggcgcg cgcgagcgcc gcgcgctgct gctggccgcc cgagagcacg
     4141 cgcccgatgc gcccggcctc gctcgccagg ccgacgcgct cgagcgcatg catcgcgcgc
     4201 tcgcgccgct cggcgcgcgg cacgcgctgc agcatgaggc ccagctcgac gttggcgcgc
     4261 accgtcatgc gcagcatgaa cgggcgctgg aacaccatgg cgatgcgcgg cgcgcccgcc
     4321 atgcgccgct ccccgccggc cagcggcacc aggccgtgca gcgcgcgcag cagcgtgctc
     4381 ttgcccgacc cgttcgcgcc gacgagcgcg atgcgctcgc cgcgcgcaac cgccaggtcc
     4441 accgcgttca aggcgacctt cggaccgaag cgcacggtgg cgccgcgaag ctccagcaac
     4501 ggcgtcatcc ggtcacccag gcgcatcgcg cggtccctgc gagagggacg gcgcggcgcc
     4561 gccgcggcgg ccgtgcgcca gcgctcacgg cagcaccccc gcctcgcgat cgccccggat
     4621 gcgccagccg cgcagcgcgc cgatgaccac gttcagcacc agcaccacgc ccagcagcac
     4681 gaacccgagc gcgagcgcca ccggcaggtc gcccttgctc gtctccagcg cgatcgcggt
     4741 ggtcatcacg cgcgtcacgc cggcgatgtt gccgcccacc atcatcaccg cgcccacctc
     4801 ggagacggcg cggccgaagg ccatgagcgc gagcgtgagg atcgccagcc gctcgtccca
     4861 cgcgatgatg agcgcgcatt cgagccgcgt ggcgccgagc gagcgcagct gctcgccgtg
     4921 gtcgcgcatc gcgtcctcga tggtctggcg cgcgagcgcg gcgacgatcg gcgtgacgag
     4981 cagggactgc gcgatgacca tcgcggcggg cgtgaagagg atgccccacg cgcccagcgg
     5041 cccggcgcgc gagagcagca ggtacacgat gaggcccacc accaccgagg gcagcgcgag
     5101 cagcgtgttg agcacgacga tcacgccgct gcgccccggg aagcgccaca ccgcgatcat
     5161 cgcgcccgcg agcagtccga gcgcggtcgc gaccaggcac gcgatgccgc tcaccgacag
     5221 ggacagcccg acgatgtgca gcagctgcgg atcgcggtcg gcgaccagcc gcgcggcggt
     5281 ggccaggctg tcgaggaagg gattcatgcg ggggcgaatc ctatcgccgc ctcgcgctcg
     5341 ccagcctcgc gctcacgtgg cgcagctgcg aaagccgcag aacccccggt cgtcctccgc
     5401 catcgcccag cggcgcaggc gcgtgtggcg cagccgcgcc ggcgtcgcga acgatgcgcc
     5461 gcgctgcgac ttcgcctgcc cgaaatgggc gcgcgacggg tcggcccacg gatcgggcac
     5521 gaacccgggg tacggccgga acgtgctcgc cgtccattcg cgcacgccgc cccaggcgaa
     5581 gccgcgcgag tacgactgcg cggccgcttc ccactccacc tcggcgggca gccggcggcc
     5641 ggcccagcgg caccaggcgt cggcctcgtg ccagctcacg tgcatcgcgg gctgcccgcc
     5701 cggcacgcgc gccaggcggc cgaagcgctg ctgcagcacg ccgccgcgca gctggtcgac
     5761 gtagcgcggc acgcgccgcc cctcgcgctg cagccaggcg agcccgtgcg gcgaccacca
     5821 gcgcggctcg tcgtagccgc cgtcctcgac gaactcgccg tactgcgacc acgtgaccgg
     5881 gtgggcgtcg atctcgaatt ccggcaccgc cacctcgtgc gcccacttct cgttgtcgaa
     5941 cacgaagccg cccggcgcgg agccgagcat ccagcgcgtg gcggggaaga acagcggctc
     6001 gcgcggcggc gtcgcgaggg ggtcgcccac gagcgcgtgg cgcgaggcga tcgcgatgcc
     6061 gaggccctgc gccgtctcgg ccagcgcctc ggcctgcagg tcctcgtgga agagcgcgag
     6121 gcggtacgcg tacagcgccg gatcggtctc ttcggcgctc tccagcagct ccagcgtgag
     6181 ctccatggtg tcggcgaggt actggcgcgt cgcggccgcg tcgggcagcg ccatcgacca
     6241 gcgctgctcg cgcgcagact gtgccgggtc gtaccagcgg tcggcctgcg ggagcaccga
     6301 aggcaggcgc ggcgcggtcg ggtcggcctc ggcgccgcgc tggcggtgca cgttgcgcgc
     6361 cacccagcgc tcctggaacc acgccacgtg gccgatgcgc cacagcggcg ggtccaggtc
     6421 cggctgcggc ggcagcgacc agggcggcgc gccgagcgcg tcctccagcg ccgcgagcca
     6481 gcgcaacgtg cggttgcgcg cgtccatgag cgccagcgac agcaggtccc gtccggcgcg
     6541 ccgcatcagc ggcgaatgca catccgcgct ttcaaggtcc accactcgat cgctcccgat
     6601 gccgtgcgga ttgtggcacc cggaccgaga taatcgcgcc ccatgccgtt gctgatcccg
     6661 ttcgcgtcca cgctctccga agccagccgg catgccctgc gcgacctcgc gctgcccaac
     6721 ctcgcgaagc tgctggcgca cagccgtccc gtggcgcgct ggggcggcga cgagtacacc
     6781 ctcacaccgc cgcacgagcg cgcgctcgcc gaggcgcagg gctggagcgg cgacgacggc
     6841 gccctgccct gggccgcgct gcacgccgcg caggacggca tcgcgcccgg cgacgacgcg
     6901 tggggcgagc tcacgccggc gcactggcac gtcggccgcg accacatctc gctcgccgac
     6961 ccgctcgcgc tcgggctcgc cgccgccgag tcgcgcaccg tgttcgacgc ggtgcgcccg
     7021 ctcttcgaga gcgagggctg gcgcatggcg tggggcgcgc ccacgcgctg gtacgccgcc
     7081 cacgaatcgc tgcacgggct gccgtgcgcc tcgctcgacc gcgtgatcgg ccgcaacatc
     7141 gacgcgtggc tcaccgatgc gccgggccga cacctgtggc cgcggctgca cagcgaagca
     7201 cagatgctgc tgcacggtca cccgatcaac gaacggcgcg agtcgcaagg cctgtcgagc
     7261 gtgaactcgg tctggctcag cggctgcggc gcaccgcgtc cagccagcgg agaagcgccg
     7321 tgc
//
LOCUS       DASRIE010000022        10495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3672776, whole genome shotgun sequence.
ACCESSION   DASRIE010000022 DASRIE010000000
VERSION     DASRIE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3672776"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="VFB53_00910"
     CDS             complement(<1..1749)
                     /locus_tag="VFB53_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85319.1"
                     /translation="MPLITLLDAQLAYGDFPLLDRAAFSMEPGERIGLIGRNGTGKSS
                     LLAVIAGAAALDDGELRKSESTRVASVEQEPKLPHAPTLRASLLLRGHIPSIIDERRR
                     WQVEARLTEFLHRFGLDEELSPDAASGGERKRAALALAFALAPDLLLLDEPTNHLDIA
                     GIEQLEALLLRQQAAIVVTHDRAFLDRVCTRILELDRGMLRSYPGNYSAYESVKAADL
                     AAEATAQRKFDKFWKEEEAWIRKGIEARRTRNEGRVLRLERLREERAARRERLGSVKL
                     SLSAGQRSGKLVAELKEVSKSFAGRPIVKDLDLLIGRGDRLGLVGANGAGKTTLIKLI
                     LGTLEPDAGSVRLGTNVQPAYFDQMREALDPGKTVMETIAPGSDWVELAGGRKHVMSY
                     LGDFLFPPRRAQSPVRMLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIESLELLEA
                     ALQDYDGTLLLVSHDRAFLDNVVTQTLAAEGGGAWKEYVGGYTDWVRQRPAATTEDKA
                     SSKAAVRGKAKAKLSYKEQRELESLPKEIEALEAEQASLAAKLALPEYFRQPAEALKA
                     DAKRNEEIEALMLKKLE"
     gene            1759..2973
                     /locus_tag="VFB53_00915"
     CDS             1759..2973
                     /locus_tag="VFB53_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85320.1"
                     /translation="MRYLPLALIALSLALSYGVWYAYSVILVALLQDYGWSRSVLAGA
                     FSVFTLVHGAANPLIGSLCDRIRPMRLMAAGGAALGLALWADSTISSPLQLYLGFGVL
                     TAAAVAAAGWIPALVQVQRDFQDKLGLALGIVSSGVGVGMLLVVPLTQLLIDAYGWRT
                     AFRALGAICLLWIVPSSLYLLSRAPARRQAQQSANPARAAPRAVTLAEALRGMPFWLM
                     LAAFFFGNVCSQTLHVHQVAYLVDQGVAGIVAASVVGVVGLSSIVGKTGGGWLSDRMD
                     RERIYLAGIAIMVAAVAALAALAGDPARWAIYGYAVLLGLGYSVTASLIPAMVSDRFS
                     GPHFGAIVGMGLLGSAAGSALGPWMAGAIFDATGSYRIAFAIAAACGVAAGAAGWRAR
                     TLRLRAMAGQAA"
     gene            3120..3419
                     /locus_tag="VFB53_00920"
     CDS             3120..3419
                     /locus_tag="VFB53_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN85321.1"
                     /translation="MRRKLTPRTVGDGERNLKVQLAVEVNRILQQRGLTQTEAAQELG
                     IVQPHVSELAHYRLDRYSAERLMQFLARLGQEVEIRIHGGASRTQRPAMRRIVIR"
     gene            3422..3919
                     /locus_tag="VFB53_00925"
     CDS             3422..3919
                     /locus_tag="VFB53_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HZN85322.1"
                     /translation="MPIAISAAGATFSSCRRWRYLLWRRWDDERPAANFLMLNPSTAD
                     EHQLDPSCSRARDFAERWGYGALIVTNLFAWRATDPRAMKRAAEPVGRSNNQAILRAA
                     RQSAIVVCAWGNHGAHLGRAATVAANLRGAGLALSALRITAQGQPAHPLYLPARLAAA
                     RWREA"
     gene            complement(3916..4104)
                     /locus_tag="VFB53_00930"
     CDS             complement(3916..4104)
                     /locus_tag="VFB53_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85323.1"
                     /translation="MPHSTRYPTHAEIEAVMARARLMRSLFVAALIRRALRRLQDAYA
                     DATVPRSSATRRFYPDIG"
     gene            4271..5158
                     /locus_tag="VFB53_00935"
     CDS             4271..5158
                     /locus_tag="VFB53_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZN85324.1"
                     /translation="MPEIAPHLHWLPLAAIALAFFSTLSVLAFWRRRAARPAPHELGR
                     SPGQSLRDALSQAGLDAAEYAALGLCMLPLAAAGATAWRGLTGAPLEPDTALVLAGAA
                     AAAQLWVLSRLWVVLRRSRRLRYGYEAELVAGQELNGLGGLGYHVFHDVPLGARAPKA
                     DHVLVGPAGVFAVETEARARAGADPEREVTYDGASLQFAQRRETAPLEQAVAQAVRVR
                     SLLAAEVGEQFAVQPVVVLPGWRVRRTDISGIPVLAASRIRHYFGRLRPQAAMSEAMI
                     ERVAQCLEARCRGSAAPVA"
     gene            complement(5155..5817)
                     /locus_tag="VFB53_00940"
     CDS             complement(5155..5817)
                     /locus_tag="VFB53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN85325.1"
                     /translation="MKRRRFVLALSALPALALAAGCADQRAHTHAVYLLVDTSGTYAE
                     EVGKAQLIVNYLLGTLQPGDSLAVARVRSRSFSEKDIIAKATFDQRPSQANAQKRVLR
                     DQVEAFTGSARSSVYTDITGGLIQGAMFLNETGAGKKTILIFSDMQEELDKAAVRNFP
                     IDLKNIRVVAVNVVKLKTDNVDPRRYLGRLEDWEKRVVAAGAREWRVINDIEHLDALL
                     KS"
     gene            complement(5814..7265)
                     /locus_tag="VFB53_00945"
     CDS             complement(5814..7265)
                     /locus_tag="VFB53_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85326.1"
                     /translation="MADSPFWVIWPGNPALSALLWFVIAMPFLYAARAPVHGLLRSLG
                     ALANGPLRLAARTLAATARDLRERNRAVLFAQGREETAERIGREFERVAALVKRDLEG
                     YPALQRKLLDEITRVEEDYKKCGEVPPPPPDWVEAVSAVAKIKPVNGNELVLRVLEEI
                     NDSIKSIHEKTLAEYRRAYESRHKILDGFMPFWRSLDKTLAQADARMTNLSDRAASID
                     AQMERYEMIVKKTDRAEHALTTSALTQFFVATLVLAVAAGGALVNFKLIALPMSEMVG
                     AGDYLTATLRTSEVAALVIIFVEAAMGLFLMETLRITHLFPKIHGLPERMRKRMMWTA
                     FVLLLCLAGIEAALALMRDMLIADKQALLQSLAATQAAAADGWIGRIPTVGQMLLGFI
                     LPFALAFVAIPLESFVNSARTVGGAALVVLARALAAALRVAGNFFKHLSRVLVTLYDI
                     LIVLPLIAERWVKQARPAGKPPRVAGLHAGERP"
     gene            complement(7344..7700)
                     /locus_tag="VFB53_00950"
     CDS             complement(7344..7700)
                     /locus_tag="VFB53_00950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN85327.1"
                     /translation="MATTTASGLIIDDKVLGKGAAAKAGDGVSVHYTGWLFYGGERGK
                     KFDSSKDRGDPFEFPLGAGQVIKGWDEGVQGMQVGGTRVLTIPPALGYGARGAGGVIP
                     PNATLVFEVELLGIET"
     gene            complement(7712..9193)
                     /gene="gshA"
                     /locus_tag="VFB53_00955"
     CDS             complement(7712..9193)
                     /gene="gshA"
                     /locus_tag="VFB53_00955"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZN85328.1"
                     /translation="MRRGIEKESLRTRPDGALALTPHAAALGSALAHPHVTTDFSESQ
                     LELITGVHPSAEACLEELTRIHQFVYRAIGEELMWAGSMPCNLPADDAIPIGRYGTSN
                     VGRAKTVYRNGLAWRYGRRMQAISGIHYNWSMPGLGNAEYFALIRNFRRHGWLLFFLF
                     GASPAVCSSFVAGRAHPLRELRPGTLHLPHATSLRMGRLGYQSDAQSSLVVSCNDLES
                     YGATLQRGLTQVYPPYDKVGIREPGRPGDGPETYRQMSTTLLQIENEFYGKIRPKRRI
                     RPGERPLHALRERGVEYVEVRLMDLDPFAPAGITLPVMRFLDVFLLHCLLSPSPPDTP
                     EDIACESRNQETVAARGREPGLVLGCFPGEMGLAQWGAQLLAECEPIAAALDAALGGD
                     AYRAAHAAQGAVLRDPALAPSARVLAAMLGEHAGSYTDFTLALSRRHRAALMAQEFPR
                     AEEERFARLAAESLAKQKAIEAADTLPFEEYRRKYLAVERLGN"
     gene            9304..>10495
                     /locus_tag="VFB53_00960"
     CDS             9304..>10495
                     /locus_tag="VFB53_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZN85329.1"
                     /translation="MNFWNRKQKALEADEVGAGGKPGRRGRSTSLAVRPIPEDALILI
                     PMRNAVLFPGVISPVAVGRSSSIAAANEALRQSRKVGFVLQRDAKTDDIGPEDLHWVG
                     TAGEIVRYVPVAEGPHHMAVQGEQRFRVAEFLEGWPFLVARVVWVPEPGDAGEEVEAR
                     FLQLKTQAAEAIKLLPNVPDELAGAVQGIESAAQLADMVANLLDVEAAEKQAILEAFD
                     LKARLDKVLAHLSERVGVLRLSKEIGDKTKKEFDERQREHVLREQMRQIQKELGEGED
                     TAAELEDLGKALEAAGMPEEALKHAKKEFKRLQRMGEASGEASMLRTWLELMAELPWK
                     AEPQKAMDIAGARQVLDDDHFGLDKVKRRILEYLAVRKLNPEGKSPILCFVGPPGVGK
                     TSLGQ"
BASE COUNT         1459 a         3728 c         3718 g         1590 t
ORIGIN      
        1 ctcgagcttc ttcagcatca gggcctcgat ctcctcgttg cgcttcgcgt ccgccttgag
       61 cgcctccgcc ggctggcgga aatactccgg cagcgccagc ttcgcggcga gcgaggcctg
      121 ctcggcctcc agggcctcga tctccttcgg cagcgactcc agctcgcgct gctccttgta
      181 ggagagcttc gccttcgcct tgccgcgcac cgccgccttc gacgaggcct tgtcctcggt
      241 cgtggcggcc ggacgctgcc gcacccagtc ggtgtagccg cccacgtact ccttccacgc
      301 cccgccgccc tccgccgcga gcgtctgcgt cacgacgttg tcgaggaaag cgcggtcgtg
      361 ggagacgagg aggagcgtcc cgtcgtagtc ctgcagcgcc gcttccagca attcgagcga
      421 ctcgatgtcg aggtcgttgg tgggttcatc gagcaccagc acgttggccg gcttggcgaa
      481 gaggcgcgcg agcagcaggc gattgcgctc gccgccggag agcatgcgca ccggcgactg
      541 cgcgcgccgc ggcggaaaaa ggaagtcgcc gaggtagctc atcacgtgct tcctcccccc
      601 ggcgagctcc acccagtccg agcccggcgc gatggtctcc atcaccgtct tccccgggtc
      661 gagggcttcc cgcatctggt cgaagtaggc cggctggaca ttggtgccga ggcgcaccga
      721 gccggcatcg ggctcgagcg tgcccaggat cagcttgatg agcgtggtct tccccgcgcc
      781 gttggcgccg acgaggccga ggcggtcgcc gcggccgatg aggaggtcca ggtccttcac
      841 gatcggcctg ccggcgaagg acttcgacac ctccttcaac tccgccacca gcttccccga
      901 gcgctgcccg gcggagagcg agagcttcac cgagcccagg cgctcgcgcc gcgccgcgcg
      961 ctcctcgcgc aggcgctcga ggcgcagcac gcggccctcg ttgcgcgtgc gccgcgcctc
     1021 gatccccttg cggatccagg cttcctcctc cttccagaac ttgtcgaact tccgctgcgc
     1081 ggtggcttcc gccgccaggt cggccgcctt caccgattcg taggcggagt agttccccgg
     1141 ataggaacgc agcatgccgc ggtcgagctc gaggatccgc gtgcagacgc gatcgaggaa
     1201 ggcgcggtcg tgggtcacga cgatcgccgc ctgctgccgg aggagcagcg cctccagctg
     1261 ctcgatgccg gcgatgtcga ggtggttggt cggctcgtcg aggagcagga ggtcaggcgc
     1321 gagcgcgaag gcgagggcga gcgccgcgcg cttccgctcc ccgccggagg ccgcatcggg
     1381 agacaactcc tcgtcgagcc cgaagcggtg caggaattcg gtcaagcgcg cctccacctg
     1441 ccagcgccgc cgctcatcga tgatggaagg gatgtgcccg cgcagcagga ggctcgcgcg
     1501 aagagtgggc gcgtgcggga gcttcggctc ctgctccacg ctcgcgacgc gagtgctctc
     1561 gctcttcctc agctcgccgt cgtcgagcgc cgccgcgccc gcgatcaccg cgaggagcga
     1621 gctcttcccc gtgccgttcc tgccgatcag gccgatgcgc tcgcccggtt ccatggagaa
     1681 cgcggcgcga tcgagcagcg ggaagtcccc gtacgcgagc tgcgcgtcga ggagcgtaat
     1741 gagcggcata ctccgattat gcgttacctc ccgctcgccc tcatcgcgct cagcctcgcg
     1801 ctctcctacg gcgtgtggta cgcctacagc gtgatcctcg tcgcgctgct gcaggactac
     1861 ggctggtcgc gctcggtgct cgccggcgcg ttctccgtct tcaccctggt gcacggcgcc
     1921 gccaacccgc tcatcggtag cctctgcgac cgcatccgcc cgatgcggct gatggcggcg
     1981 ggcggcgcgg cgctcggcct cgccctgtgg gccgacagca ctatcagcag tccgctgcag
     2041 ctgtacctgg gcttcggcgt tctcaccgcc gcggcggtgg ccgccgcggg ctggatcccg
     2101 gccctggtac aggtgcagcg cgacttccag gacaaactcg gcctggccct cggcatcgtc
     2161 agctcgggcg tgggcgtggg catgctcctc gtggtgccgc tcacccagct cctcatcgac
     2221 gcctacggct ggcgcaccgc gttccgggca ctcggcgcca tctgcctgct gtggatcgtg
     2281 ccttcgtcgc tctacctgct ctcgcgcgcg ccggcgcgcc gccaggccca gcagagcgcg
     2341 aacccggccc gcgccgcgcc gcgcgcggtg acgcttgcgg aagcgctgcg cggcatgccc
     2401 ttctggctga tgctcgcggc cttcttcttc ggcaacgtct gctcgcagac cctgcacgtg
     2461 caccaggtgg cctacctcgt cgaccagggc gtggccggga tcgtcgccgc ctcggtggtg
     2521 ggcgtggtgg gcctgtcgag catcgtcggc aagacgggcg gcggctggct ctccgaccgc
     2581 atggaccgcg agcgcatcta cctcgccggc atcgccatca tggtggccgc ggtggcggcg
     2641 ctcgccgcgc tcgccggcga ccctgcgcgc tgggcgatct acggctacgc cgtgctgctg
     2701 ggcctgggct actccgtgac ggcgtccctc atcccggcga tggtgagcga ccgcttcagc
     2761 ggcccgcact tcggcgccat cgtcggcatg ggcctgctcg gcagcgcggc gggcagcgcg
     2821 ctcggcccct ggatggccgg ggcgatcttc gatgccaccg gcagctaccg catcgccttc
     2881 gccatcgccg ccgcctgcgg cgtcgccgcg ggcgccgcgg gctggcgcgc gcgcacgctg
     2941 cggttgcgcg ccatggcggg ccaggcggcc tgagccccgc gcgcgcaatt gccgcgccgc
     3001 cggttcaggc gagcgggcct gccgtttcgc ccacgagtcc gtgcgcgaga gccttgccgt
     3061 ccaccgagtc ttcgatccgc ggtttcgccg cgctataccg ttataggtat aattgcgcga
     3121 tgcgcaggaa attgaccccc cgcaccgtcg gcgacggcga gcgcaatctc aaggtccagc
     3181 ttgccgtcga agtgaaccgc atcctgcagc agcgcggcct gacgcagacg gaggcggcgc
     3241 aggaactggg gatcgtgcag ccgcacgtct ccgagctcgc gcactaccgc ctggaccgct
     3301 attcggcgga gcgcctgatg cagttcctcg cacggcttgg gcaggaagtg gagatccgca
     3361 tccatggcgg cgcgtcgcgc acgcagcggc ccgccatgcg ccgcatcgtg atccgctagc
     3421 catgcccatc gccatcagcg ccgcgggcgc gaccttctcc tcctgccgcc gctggcgcta
     3481 cctcctgtgg cggcgctggg acgacgaaag gcccgccgcc aacttcctca tgctcaaccc
     3541 cagcaccgcc gacgagcacc agctcgaccc gtcatgctcg cgcgcgcgcg acttcgccga
     3601 gcgctggggc tacggcgcgc tcatcgtcac caacctcttc gcttggcgcg ccaccgaccc
     3661 gagggcgatg aagcgcgccg ccgagccggt gggccgcagc aacaaccagg cgatcctgcg
     3721 cgcggcgcgc cagtcggcga tcgtggtctg cgcctggggc aaccacggcg cccacctcgg
     3781 gcgcgccgcg acagtggccg ccaacttgcg cggcgcgggg cttgcgctca gcgccctgcg
     3841 catcaccgcg cagggccagc ccgcgcaccc gctctacctg ccggcgcggc tcgccgccgc
     3901 gcgctggcgc gaggcctagc cgatgtccgg gtagaagcgc cgcgtggcgc tgctgcgcgg
     3961 caccgtggcg tcggcatagg catcctgcag gcgccgcagc gcccgcctga tcagcgcggc
     4021 cacgaagagc gaacgcatga ggcgcgcgcg agccatgacc gcttcgattt cggcatgcgt
     4081 gggatagcgt gtggaatgcg gcatgcgggt tctcctcttt ggtttaggcc ccagccatgc
     4141 acggggcgtg ccagcgcggc aggatgcgcc tgatatgtga cgggagatgg tttccggcgc
     4201 ggaaaatctg tcgccgatcg gcaacagtcc agcgctacat cttcgcgccg gcgaggagta
     4261 cagtctgcgc atgccggaaa tcgcgccgca cctgcactgg ctcccgctcg cggccatcgc
     4321 gctcgcgttc ttcagcacct tgtcggtgct cgctttctgg cgccgccgcg ccgcgcgccc
     4381 cgcgccgcac gagctggggc gcagccccgg ccagtcgctg cgcgacgcgc tgtcgcaggc
     4441 ggggctcgac gccgcagaat atgccgcgct cgggctgtgc atgctgccgc tggcggccgc
     4501 cggcgccacc gcgtggcgcg gcctgaccgg tgcgccgctc gagcccgaca ccgcgctcgt
     4561 cctcgccggg gcggccgccg cggcgcagct ctgggtcctg tcgcgcctgt gggtggtgct
     4621 gcgccgctcg cgccggctgc gctacggcta cgaggccgag ctggtggccg ggcaggagct
     4681 gaacggcctg ggcggcctgg gctaccacgt gttccacgac gtgccgctcg gcgcgcgcgc
     4741 gcccaaggcc gaccacgtgc tggtcggccc ggcgggcgtg ttcgcggtgg agaccgaggc
     4801 gcgcgcgcgc gcgggcgccg acccggagcg cgaagtcacc tacgacgggg cgagcctgca
     4861 gttcgcgcag cgccgcgaga ccgcgccgct cgagcaggcg gtcgcgcagg cggtgcgcgt
     4921 gaggagcctc ctggcggccg aggtgggcga gcagttcgcg gtgcagccgg tggtggtgct
     4981 gcccggctgg cgcgtgcggc gcaccgacat ctcgggcatc ccggtgctgg cggcgagccg
     5041 catccggcac tacttcgggc gcctgcggcc gcaggcggcg atgagcgagg cgatgatcga
     5101 gcgcgtcgcg cagtgcctcg aggcgcgctg ccgcggcagc gcggcgcccg tcgcctagct
     5161 cttcagcagc gcgtcgaggt gctcgatgtc gttgatcacg cgccactcgc gcgcgccggc
     5221 ggcgaccacc cgcttctccc agtcctccag ccgccccagg tagcgccgcg ggtcgacgtt
     5281 gtcggtcttc agcttcacca cgttcaccgc caccacgcgg atattcttca ggtcgatggg
     5341 gaagttgcgc accgcggcct tgtccagctc ctcctgcatg tcggagaaga tgaggatggt
     5401 cttcttgccg gcgccggtct cgttgaggaa catcgccccc tggatgaggc ccccggtgat
     5461 gtcggtgtag acgctgctcc gcgccgagcc ggtgaaggcc tcgacctggt cgcgcagcac
     5521 gcgcttttgc gcgttggcct gcgaggggcg ctggtcgaag gtggccttgg cgatgatgtc
     5581 cttctcgctg aagctgcggc tcctgacgcg cgccacggcg agcgaatccc ccggctgcag
     5641 ggtgccgagc aggtagttga cgatcagctg ggccttgccg acctcctcgg cgtaggtgcc
     5701 cgaggtgtcc accagcaggt acaccgcgtg cgtgtgggcg cgctggtcgg cgcagcccgc
     5761 ggcgagcgcc agggccggga gcgcggagag cgcgaggacg aaccggcggc gtttcatggg
     5821 cgttctcccg cgtgcaggcc ggcgacccgc ggcggcttgc ccgccgggcg cgcctgcttc
     5881 acccagcgct cggcgatcag cggcagcacg atgaggatgt cgtagagcgt caccagcacg
     5941 cgcgacaggt gcttgaagaa gttgcccgcc acgcgcagcg ccgcggcgag cgcgcgcgcg
     6001 agcaccacca gcgcggcgcc gcccacggtg cgcgccgaat tgacgaagga ctccagcgga
     6061 atcgccacga aggcgagcgc gaagggcagg atgaagccga gcagcatctg ccccacggtc
     6121 gggatgcgcc cgatccagcc gtccgcggcg gcggcctgcg tcgccgccag cgactgcagc
     6181 agcgcctgct tgtcggcgat cagcatgtcg cgcatcagcg ccagggccgc ctcgatgccc
     6241 gcgaggcaca ggagcaggac gaacgccgtc cacatcatgc gcttgcgcat gcgctcgggc
     6301 aggccgtgga tcttcgggaa gaggtgcgtg atgcgcaggg tctccatgag aaagaggccc
     6361 atcgccgcct cgacgaagat gatcaccagc gccgccactt ccgaggtgcg cagggtcgcc
     6421 gtgaggtagt ccccggcgcc caccatctcc gacatcggca gcgcgatcag cttgaagttg
     6481 accagcgcgc cgccggcggc caccgccagc accagcgtgg cgacgaagaa ctgcgtcagc
     6541 gccgaggtgg tgagggcgtg ctcggcgcgg tcggtcttct tcacgatcat ctcgtagcgc
     6601 tccatctgcg cgtcgatcga cgcggcgcgg tcgctgaggt tggtcatgcg cgcgtcggcc
     6661 tgcgccaggg tcttgtcgag cgagcgccag aacggcatga agccgtcgag gatcttgtgg
     6721 cgcgactcgt aggcgcggcg gtactcggcg agcgtcttct cgtggatcga cttgatcgag
     6781 tcgttgatct cctccagcac gcgcagcacc agctcgttgc cgttgaccgg cttgatcttg
     6841 gccaccgcgc tcaccgcctc gacccagtcg ggcgggggcg gcggcacctc gccgcacttc
     6901 ttgtagtcct cctcgacgcg cgtgatctcg tccaggagct tgcgctgcag tgcgggatag
     6961 ccctcgaggt cgcgcttcac cagcgcggcg acgcgctcga actcgcgccc gatgcgctcg
     7021 gcggtttcct cgcgcccctg cgcgaacagc accgcgcggt tgcgctcgcg caggtcgcgg
     7081 gcggtggcgg cgagggtgcg cgcggcgagg cgcagcgggc cgttggccag cgcgccgagg
     7141 gaacggagca gtccatgcac cggagcgcgt gccgcataca ggaacggcat cgcgatcacg
     7201 aaccacagca gcgcggaaag cgccgggttg cctggccaga tcacccagaa cggagaatcc
     7261 gccatagggc actcctttca acagctcaga gttgccccct acggcccata gtctagcgca
     7321 aaccgggtgc ttcgcagccg gtactaagtc tcgattccga gtagttcgac ctcgaaaacc
     7381 agtgtcgcat ttggcggtat cacgccgccc gcgccgcgcg cgccgtagcc gagcgcgggc
     7441 ggaatggtaa gcaccctcgt tccgcccacc tgcatgccct gcacgccctc gtcccagccc
     7501 ttgatgacct ggccggcgcc caaaggaaat tcaaaggggt cgccgcggtc cttgctcgaa
     7561 tcgaacttct tgccgcgctc gccgccgtag aacagccacc cggtgtagtg caccgagacg
     7621 ccgtcgccgg ccttggcggc cgcgcccttg ccgaggacct tgtcgtcgat gatcagtccg
     7681 cttgcggtgg tggtcgccat cttttctcct gtcagtttcc cagccgctcc acggccaggt
     7741 acttcctgcg gtattcctcg aagggcaggg tatccgccgc ctcgatggcc ttctgcttcg
     7801 ccagcgattc ggccgccagg cgcgcgaagc gctcttcttc cgcacgcggg aattcctgcg
     7861 ccatcagcgc cgcgcggtgg cggcgcgaca atgccagcgt gaagtcggtg taggaccccg
     7921 cgtgctcccc gagcatggcc gccagcacgc gcgccgaggg cgcgagcgcc gggtcgcgca
     7981 gcaccgctcc ttgcgcggcg tgcgccgcgc gataggcgtc gccgcccagc gccgcatcga
     8041 gcgcggcggc gatcggctcg cactcggcga gcagctgcgc gccccattgc gcgaggccca
     8101 tctcgccggg aaagcagccc agcacgaggc ccggctcgcg cccgcgcgcc gccaccgtct
     8161 cctggttgcg cgactcgcag gcgatgtcct cgggcgtgtc gggcgggctc ggcgagagca
     8221 ggcaatgcag caggaacacg tcgaggaagc gcatcaccgg cagggtgatg ccggcgggcg
     8281 cgaaggggtc gaggtccatc aggcgcactt cgacgtactc cacgccgcgc tcgcgcagcg
     8341 catgcagcgg gcgctcgccg ggccggatgc ggcgcttggg ccggatcttg ccgtagaact
     8401 cgttctcgat ctgcagcagc gtggtggaca tctgccggta ggtctccggg ccgtcgcccg
     8461 gcctgccggg ctcgcggatg ccgaccttgt cgtagggcgg gtacacctgg gtgaggccgc
     8521 gctgcagcgt ggcgccgtag gactccaggt cgttgcagct caccaccagc gaggactgcg
     8581 cgtcgctctg gtagcccagc cgccccatgc gcagcgacgt ggcatgcggc aggtgcagcg
     8641 tcccggggcg cagctcgcgc agcgggtgcg cgcggcccgc gacgaagctc gagcacaccg
     8701 cgggcgaggc gccgaaaagg aaaaacagca gccagccgtg gcggcggaaa ttgcggatca
     8761 gcgcgaagta ctcggcgttg cccaggcccg gcatcgacca gttgtaatgg atgccgctga
     8821 tcgcctgcat gcgccggccg tagcgccagg cgaggccgtt gcggtacacc gtcttcgcgc
     8881 gccccacgtt ggaggtgccg tagcggccga tgggaatggc gtcgtccgcc ggcaggttgc
     8941 agggcatgct gcccgcccac atcagctcct cgccgatggc gcggtagacg aactggtgga
     9001 tgcgcgtgag ctcctccagg caggcctcgg cgctcgggtg cacgccggtg atcagctcca
     9061 gctgcgactc gctgaaatcg gtggtgacgt gcggatgcgc gagcgccgat cccagcgcgg
     9121 cggcgtgcgg ggtgagggcg agggcgccgt cgggccgcgt gcgcagcgac tccttctcga
     9181 tgccgcggcg catgcccttg agggcagcgg ggtcaattgc gcgcaatttt tcttcgagtc
     9241 ctcccatgtg ccaaaggtag cagaccgctt gtttggccgg aaaccgccca catgtggacc
     9301 ggcatgaact tctggaaccg gaagcagaaa gcccttgagg ccgacgaggt cggcgccggc
     9361 ggcaagcccg gccggcgcgg ccgctcgact tccctcgcgg tgcggccgat ccccgaggac
     9421 gcgctgatcc tcatcccgat gcgcaacgcg gtgctgttcc ccggcgtcat ctcgccggtg
     9481 gcggtgggcc gcagctcctc catcgccgcg gcgaacgagg ccctgcgcca gtcgcgcaag
     9541 gtgggcttcg tcctgcagcg cgatgcgaag accgacgaca tcggcccgga ggacctgcac
     9601 tgggtcggca ccgcgggcga gatcgtgcgc tacgtgccgg tggccgaagg gccgcaccac
     9661 atggcggtgc agggcgagca gcgcttccgc gtcgccgagt tcctcgaggg ctggccgttc
     9721 ctggtggcgc gcgtggtgtg ggtgccagag ccgggcgacg cgggcgagga ggtggaggcg
     9781 cgcttcctgc agttgaagac gcaggccgcc gaggcgatca agctgctgcc caacgtcccc
     9841 gacgagctcg ccggcgcggt gcagggcatc gagtcggccg cgcagctggc cgacatggtg
     9901 gcgaacctgc tcgacgtgga agccgcggag aagcaggcga tcctcgaggc cttcgacctg
     9961 aaggcgcgcc tggacaaggt gctcgcgcac ctctccgagc gcgtgggcgt gctgcgcctg
    10021 tcgaaggaga tcggcgacaa gaccaagaag gaattcgacg agcgccagcg cgagcacgtg
    10081 ctgcgcgagc agatgcgcca gatccagaag gagctgggcg agggcgagga cacggccgcg
    10141 gagctcgagg acctgggcaa ggcgctcgag gccgcgggca tgcccgagga ggcgctcaag
    10201 cacgcgaaga aggagttcaa gcgcctgcag cgcatgggcg aagcctcggg cgaggcctcg
    10261 atgctgcgca cctggctgga gctgatggcc gaactcccct ggaaagccga gccgcagaag
    10321 gcgatggaca tcgccggggc gcggcaggtg ctcgacgacg accacttcgg gctggacaag
    10381 gtgaagcgcc gcatcctcga atacctggcg gtgaggaagc tcaaccccga gggcaaaagc
    10441 cccattcttt gcttcgtcgg gccgccgggg gtgggcaaga cctcgctcgg ccagt
//
LOCUS       DASRIE010000023         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2893862, whole genome shotgun sequence.
ACCESSION   DASRIE010000023 DASRIE010000000
VERSION     DASRIE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893862"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFB53_00965"
     CDS             complement(<1..225)
                     /locus_tag="VFB53_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85330.1"
                     /translation="MRAVLSSLPQVAVMGAVVATLMFLPIGVSLAMVLGLFGYSLESV
                     VTFGGAFNIVLGLVAWWLLIFGGSCIYAACA"
     gene            complement(232..888)
                     /locus_tag="VFB53_00970"
     CDS             complement(232..888)
                     /locus_tag="VFB53_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85331.1"
                     /translation="MKTLVRDPFKRAGAEDWTLERITQLSVADIKQLRENAQRLNEDT
                     VVALCSEALNSAPPRARARAGSKARPNTKARRLIPRSSAFETRGVWVEDRRASWGGVR
                     KSDGEVVLALWVDAIESANGGCSYLLWAPNVKGSRPWSDTPAGKERLEHCRRAAERGR
                     AEGLLVYGERLAGHQPEEKAYAVHGVDPETVVILRVEARGAEYWAVWGRKAVASAQPG
                     "
     gene            complement(885..1223)
                     /locus_tag="VFB53_00975"
     CDS             complement(885..1223)
                     /locus_tag="VFB53_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85332.1"
                     /translation="MRDDDGVVVFAMPAERIRVDDWGCSCPLWAPAARGARVVAAADL
                     EKLRHCRMALRQGMAEGFVLHAGEAPGEQRASLSLRVVRAGQEYWARWGCAARAGSAA
                     EQPWSAQVRI"
     gene            1412..2176
                     /locus_tag="VFB53_00980"
     CDS             1412..2176
                     /locus_tag="VFB53_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN85333.1"
                     /translation="MSVHPLKSPSTDSVRQAKVHLAAAHRLAALHELEEGIDNHFTVT
                     LPGSDERYLILPFGLHWSEARASDMIVFDESGATLEGEGTVELSAQCIHAPIHRICGA
                     RVVMHTHQTWALALNMLKDNRLLPASQTAAFFHGHVAYDDTYAGTADFPEEGERLARV
                     IGDKQVLFMKNHGVLVTGANVAQAYRRLYRLERVCRAQVLAMGTGRPLEVLSEEIVAR
                     VQAPPPNDRHSRAERERLFFEAMMRILDRVNPGYAD"
     gene            complement(2185..2364)
                     /locus_tag="VFB53_00985"
     CDS             complement(2185..2364)
                     /locus_tag="VFB53_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85334.1"
                     /translation="MDLVYFTVIAIGLYFGADWLLERMERARGKRFENRQVVFFAIIL
                     PLALATFWLVRGLSR"
     gene            complement(2337..>2889)
                     /locus_tag="VFB53_00990"
     CDS             complement(2337..>2889)
                     /locus_tag="VFB53_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HZN85335.1"
                     /translation="YRTQTWLMRIGGGLAIVPFAAAVALALLKSPRPSAATAPLKAAL
                     AWSLALFGAGGLIGLAISGSNVKIPAHYHGSIVGVTLAFMGLVYYLLPRMGFGRPQGR
                     LARLQPALYGGGQLLHIAGLLWSGGYGVQRKLAGGEQVLRSAEQVAGMGLMGLGGLLA
                     VAGGILFALVVLRALWTSSTSRS"
BASE COUNT          454 a         1077 c          963 g          395 t
ORIGIN      
        1 cgcgcacgcg gcgtagatgc aggagccgcc gaagatcagc agccaccacg cgacgagccc
       61 cagaacaatg ttgaacgcgc cgccaaacgt gacgacgctc tcgagcgaat aaccgaacag
      121 accgagcacc atggcgagcg acacgccgat cggcagaaac atcagggtcg ccaccaccgc
      181 gcccatcacg gcgacctgcg gcagcgagct cagcacggcg cgcaccgcgc gctagcccgg
      241 ctgcgccgaa gcgacggcct tcctgcccca gaccgcccag tactccgcgc cgcgcgcttc
      301 cacccggagg atcaccaccg tctccggatc gacgccgtgc accgcgtaag ccttctcttc
      361 cggctggtgc ccggcgaggc gctcgccgta gaccagcagt ccttccgcgc gcccgcgctc
      421 cgccgcgcgc cggcaatgct ccaggcgctc cttgcccgcg ggcgtgtcgg accacgggcg
      481 cgagcccttc acgttgggcg cccacagcag gtagctgcag ccgccgtttg ccgactcgat
      541 cgcgtcgacc cacagcgcga gcaccacctc gccgtcggac ttgcgcacgc cgccccagct
      601 cgcgcggcga tcctcgaccc acaccccgcg cgtctcgaag gcgctcgagc gcgggatcag
      661 ccggcgcgcc ttggtgttgg ggcgcgcctt gcttccggcg cgcgctcgcg cgcgcggcgg
      721 cgcggaattc agcgcctcgc tgcacagcgc caccaccgta tcctcgttca gccgctgcgc
      781 gttctcgcgc agctgcttga tgtccgccac gctgagctgc gtgatgcgct cgagcgtcca
      841 gtcttccgcg ccggcgcgct tgaacgggtc gcgcacgagg gtcttcaaat gcgcacctgc
      901 gcgctccagg gctgctcggc cgccgagccg gcgcgcgcgg cgcagcccca tcgcgcccag
      961 tattcctggc cggccctgac tacgcgcagc gaaaggctcg cgcgctgctc gcccggcgct
     1021 tcgccggcgt gcagcacgaa gccttcggcc attccctggc gcagcgccat gcggcagtgg
     1081 cgcagcttct cgaggtcggc ggcggcgacc acgcgcgcac cgcgcgccgc gggcgcccac
     1141 agggggcagc tgcagcccca gtcgtcgaca cggatccgct ccgccggcat cgcgaacacc
     1201 accacgccgt cgtcatcgcg cacgcgggaa tgcgcggcgt tccacatcgg gatgattcca
     1261 cctgccattt tgcttcgctc ctgtgactga attgcgaagc agggagggag agaccgcgga
     1321 gggcttctag cgcaacgctt cgtgtctctt tatacaccca attgccggat tccgctgctc
     1381 ttttctcctt cggcgctggt acagtcgggc tatgtccgtc catccgctga agtcgccgtc
     1441 gaccgattcc gttcgccagg cgaaggtcca cctcgccgcc gcgcaccgcc tcgcggcgct
     1501 gcacgagctc gaggagggca tcgacaacca tttcaccgtc acgctgccgg gcagcgacga
     1561 gcgctacctg atcctgccgt tcggcctgca ctggtccgag gcgcgcgcga gcgacatgat
     1621 cgtgttcgac gagtccggcg cgacgctcga gggcgagggc acggtcgagc tctcggcgca
     1681 gtgcatccac gcgccgatcc atcgcatctg cggcgcgcgc gtggtgatgc acacgcacca
     1741 gacctgggcg ctcgcgctca acatgctgaa ggacaaccgc ctgctgccgg cgagccagac
     1801 cgcggcgttc ttccacggcc acgtggcgta cgacgacacc tatgccggca ccgccgattt
     1861 cccggaagaa ggcgagcgcc tcgcgcgcgt gatcggcgac aagcaggtgc tcttcatgaa
     1921 gaaccacggc gtgctggtca ccggcgcgaa cgtggcgcag gcctaccgcc ggctctaccg
     1981 gctggagcgc gtctgccgcg cgcaggtgct cgcgatgggc accgggcggc cgctcgaggt
     2041 gctgtccgag gagatcgtgg cgcgggtgca ggcgccgccg ccgaacgacc ggcactcgcg
     2101 tgccgagcgc gagcgcctgt tcttcgaggc gatgatgcgc atcctcgacc gggtcaaccc
     2161 gggctacgcc gactaggccg caagtcagcg cgaaaggccg cgcaccagcc agaatgtcgc
     2221 gagcgcgagg ggcaggatga tggcgaagaa cacgacctgc cggttctcga agcgcttgcc
     2281 gcgcgcccgc tccatgcgct cgagcagcca gtcggcgccg aaatacagcc cgatcgctat
     2341 gaccgtgaag tagacgaggt ccatagcgcg cgcagcacga cgagcgcgaa caggattccg
     2401 ccggcgaccg cgagcaggcc gcccaggccc atcagtccca tgccggcgac ctgctcggcg
     2461 ctgcgcagca cctgctcgcc gccggcgagc ttgcgctgca cgccatagcc gccggaccac
     2521 agcagcccgg cgatgtgcag gagctggccg ccgccgtaga gcgccggctg gagccgcgcc
     2581 agccggccct gcggacggcc gaaccccatg cgcggcagca ggtaatacac cagccccatg
     2641 aaggcgaggg tcacgccgac gatgctgccg tggtagtgcg ccgggatctt gacgttgctg
     2701 ccgctgatcg ctagcccgat caggccgccg gcgccgaaga gcgcgagcga ccaggcgagc
     2761 gcggccttga gcggcgcggt tgcagccgaa ggcctggggc ttttcagcaa tgcgagcgcg
     2821 accgcggcgg cgaagggcac gatcgccagg ccgccgccga tgcgcatcag ccaggtctgc
     2881 gtgcggtag
//
LOCUS       DASRIE010000024        16468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1584827, whole genome shotgun sequence.
ACCESSION   DASRIE010000024 DASRIE010000000
VERSION     DASRIE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584827"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFB53_00995"
     CDS             <1..446
                     /locus_tag="VFB53_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85336.1"
                     /translation="FNLLARTPAVENVELPLVYAGIAKRERLEKAMKMLKQVGLADRA
                     EHHPAQLSGGQQQRVAIARALVTEPLLILADEPTGALDSRTSLEIMALLQNLNRQGMT
                     VVLVTHEPDVARFAHRILGFRDGKVVRDEPNQPADASEHLHLEAA"
     gene            443..1666
                     /locus_tag="VFB53_01000"
     CDS             443..1666
                     /locus_tag="VFB53_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85337.1"
                     /translation="MTLLRIALRALAVNKLRSALTMLGIVIGVGAVIVMIAVGSGAQE
                     RVEEQIRALGSNLLLVMPGSTTAGGVRMGFGSGSTLTEDDVVALNRDIPEALAAPALR
                     GTAQAVWGNSNWSTQIFGITPDYLDVRQWPLVSGRVFEPAEMAGAAKVCLIGATVARQ
                     LFGDADPLDQSVRIKRVPFTVIGVLDVKGQSMMGTDQDDLILVPIKTARSRVLGTASA
                     ARNRTVGTIWIKAADGVDTRRIEEQVRSLLRQRHRLPMGADDDFSLRNLAEVMAAQEA
                     SSRVLALLLAAVASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARTRDILGQFLV
                     EAVTLSLIGGLIGVALGIGGSVAIAQLAGWRIALSAEAIVLAVAFAFVIGVFFGFYPA
                     RKAARLNPVEALRFE"
     gene            1695..2570
                     /locus_tag="VFB53_01005"
     CDS             1695..2570
                     /locus_tag="VFB53_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN85338.1"
                     /translation="MTIAEFVYTTLLKPRLLRRAANAAIRLSLPERARVGGAMVWLNP
                     QDPVVSGALTFGVYERGEIAFFRSRFRADMTLVDVGANVGLYSALALSTPGFRGRVLA
                     IEPHSESRAYLHKTIESNAAQAPPGAALICGLAASDRAETVTLYQNPENKGDNRLYPD
                     PLLRGAETVDADTVDNICRRHGIASAQFIKIDVQGAEAKVVRGATELLAASRDCILMT
                     EFWPYGLSRCGTDGLAYLEILQDLGLRLYQLSGSRGVLAPLSSPRALVERTHGRRYAN
                     LVAVKGNLVEALRLE"
     gene            2567..3547
                     /gene="galE"
                     /locus_tag="VFB53_01010"
     CDS             2567..3547
                     /gene="galE"
                     /locus_tag="VFB53_01010"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZN85339.1"
                     /translation="MRVLVTGGAGYIGSHMVQLLLERGAEVTVLDDLSTGHADAVRGG
                     HFVRGDIADRHATGTLLRERGIEAVIHFAAAALVGESTADPPKYYARNVGGTAALLQA
                     MREAGVARMVFSSTCAIYGNPQRTPIDEAHPAQPVNPYGRSKLAVEGMLAECAAAYGL
                     RAASLRYFNAAGADPQARLGERHEPETHLIPLVLQAASGRRPEVSVYGNDWPTRDGTC
                     VRDYIHVDDLCVAHLLALEWLASEGRCEAFNLGNGEGATVLEVIAAAKRVTGRDFAVR
                     FGARRAGDPPSLVADAAKARRVLGWEPRHPAIDGIVRDAWNFERRRPPAS"
     gene            complement(3544..4287)
                     /gene="tfpZ"
                     /locus_tag="VFB53_01015"
     CDS             complement(3544..4287)
                     /gene="tfpZ"
                     /locus_tag="VFB53_01015"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HZN85340.1"
                     /translation="MSRWRAAGTHLAISAAIAAGVLALMLAYWYPPPLFGVMGGSGLA
                     LILIGVDVVMGPALTLIVFKSGKPGLKFDLAVIAACQLAALVYGCHVISLARPAFIVF
                     VKDQFQVVTVSELAPERLAEARYPQFRQPSWAGPQLVFGEWPTEAADQQRLIDAVIHL
                     EDLQHFPKHYAPYEQGRTELLSQALPLAQVHKVEPEVAKVIDAWLASSGSQADSLRYL
                     RLRGRRGWVAVLIDRVTAQPVKLLMTERF"
     gene            complement(4339..5208)
                     /locus_tag="VFB53_01020"
     CDS             complement(4339..5208)
                     /locus_tag="VFB53_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HZN85341.1"
                     /translation="MIITNLVGGLGNQMFQYAFGRALALRHGVALRLDARWFGYYRLH
                     AFALRKAFALPVEKARAAELRAVLGWRRPVRVLGVLRRPAFSGLRGARLLVDDLRQAP
                     ALTVAGAPATCYLLGYWQSESYFAAAAGEVRRDFRFPALDGANRAWAQRIGDSIAVSV
                     HVRRADYTDPATQAVHGLCTPDYYARAMAHVAERAPGAQFFVFSDDLAWARAALAIPH
                     RHHFVEGNGPEGNFHDMHLISLCRHHVIANSSFSWWGAWLCSQAGKIVVAPARWMARA
                     DPPEIVPASWARL"
     gene            complement(5218..5871)
                     /locus_tag="VFB53_01025"
     CDS             complement(5218..5871)
                     /locus_tag="VFB53_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN85342.1"
                     /translation="MDTLLERIVRRFRAHVLRDPFLLAVQRWHRDRGDDTLRLEFPLT
                     RDSLVFDVGGFRGDWAHEIRRRYGCRIDIFEPMPAFLAELRTRFAGEAGIRIFPWALS
                     DASGPVTFYECADASGALARRGGAAVRCEKKDVAEVLAALPGDIDLMKVNIEGGEYEV
                     LPALARGGIERVKRLLVQFHNFVPDAAARRREIRAALGRTHREEWCYPFVWEAWART"
     gene            complement(5871..7223)
                     /locus_tag="VFB53_01030"
     CDS             complement(5871..7223)
                     /locus_tag="VFB53_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85343.1"
                     /translation="MSVWHSPVFMTWGAWGARALSFVTLLPLTLALLPAEEVAVWFLF
                     FSMQTLAALVDFGFAPTFVRAVAYANAGAGSPGMDAVIRTMRSVYARVALAGAFLAAV
                     PGTFALWNPIAQLREPSHAWLAWAMMACATVIWLRSLKYWAFLHGSERTAVLRRAEIA
                     VGLSSLAVSCAVLLAGGGLAGLACIHLAAAVAGYALNRALARRHAPAGAWDGPASAQP
                     AVMHTVFPAAWRSGLGASITYGVVQGSGVFYAQLAAAAEVASYLLALRLMQEMGAVSS
                     VPLSARLPALARLYADGRRAEVVALAGRGMLWGNWLFLGGVAALGVAGEGLLASIGGR
                     TAFVPGEVWWLLGAAFLVERIGTLHLELYGTTNRVAVHVANGVCGLVMIAAMALAYRW
                     LGVAGLPLGMLAAYAGFYTPFSMRQSYRAFGLAARFDLAASAAPLAICLGVLLAVLAR
                     "
     gene            7287..8261
                     /locus_tag="VFB53_01035"
     CDS             7287..8261
                     /locus_tag="VFB53_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN85344.1"
                     /translation="MPEPLLTVVLPVRNAAAYLQRALDSLQTQTFGDFRVLAIDDGST
                     DGSGEILQSLRDPRFSVIRREARGLVHTLNEGLERAATPYVARMDADDCSLPERFARQ
                     VQALEADPALALVGSQARVIDAAGAPMGQLPALTEHAAIRRALAVTNCFVHGSTVFRR
                     EIALRAGAYRASAALVEDYDLWQRIAELGRVANHAEVLYEWRATPAGASRSRRAEQRA
                     AAERVADGIWQAWFGEAGPAPREAWPEIWRPGSEPLFASNLHLLFARGYLRRGRRALA
                     FSHVRAALAFRPHNMAGWAYAGAFPLPPRWFSALEGHARTLMERQRGW"
     gene            8258..9370
                     /locus_tag="VFB53_01040"
     CDS             8258..9370
                     /locus_tag="VFB53_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN85345.1"
                     /translation="MRPLPQPVCLVQPTLFPEASRVDIYEYGCALARLGVETHVVVSE
                     NHAGQLPPGLIVHQTGFAPRNTPARWWRFAAFARATVRRLNFGIVHLFNPSPATYLLG
                     WMLRREPRRPCIIYDLRTGGLGHSPVAMLVNAMARTAPAFADGLIAVTGALGNHLLGA
                     GRFREVPIGVNLESFRPRARAPRAGDEFVFVYAGTLSRNRALGNMLAAFARVAEEHPR
                     ARLVIAGDGDDRDRLEAFVAAHGLRHAVSFLGKRPHAEVPDLLAAADCGLSYVPDVPW
                     FQPQAQLKTLEHFAAGLTTVAVRTAGNRQYWDGLPAELLTADDPESYAAGMRCALTGT
                     YDPARFRRVAEANSWDRITRERLVPFYEATLAARKP"
     gene            complement(9345..10154)
                     /locus_tag="VFB53_01045"
     CDS             complement(9345..10154)
                     /locus_tag="VFB53_01045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85346.1"
                     /translation="MKDSLPNRRRIPLPAKLRLAGMALRESGIRWFVLFAAYYVASAF
                     AGRAFTAMENLRRSRGVPGLNSAKLNKAIWEAWDWSGEGREFGTGEWKDSVIRCLLEP
                     HLAAGGATLEIGPGSGRWTEALIDRSARFIGVDISEACVNLCRERFAARANASFMLGS
                     GLDLGGIESGSIDALWSFDVFVHINTAEVRGYAAEFARVLRPGGRGAIHHGAVAGAAG
                     GWRSDLTQQALLDLLGQNGLRVLHSFAEWQDGDTPQRLDFEDAFTVFERPA"
     gene            complement(10144..11061)
                     /locus_tag="VFB53_01050"
     CDS             complement(10144..11061)
                     /locus_tag="VFB53_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85347.1"
                     /translation="MASELSDAAWRALACPGCGGALVRSGAGAACGACSARYARSKSG
                     ALDLRLQQPKSVAHEFRLGVPLAVAPEEFRELPRNPSPQVDFGGRAAPYHLTPELLSH
                     FPRAKATGELMLDLGCGSALHREVCEAAGFEYLGLDHDAAGASVLGDAHALPFKDASF
                     AFVLSIAVLEHLRFPAVALREARRVMRPGAVFLGTVAFLEPFHADSYYHHTHLGLRSG
                     LEEAGFAVDYVCPAAGWSGLLPLAQMGLFPRMPAVMVKSLLLPLQLLHRVWWALGRRF
                     DPRATEALRVLSTSGAFSFVARTPGRDER"
     gene            11114..12034
                     /locus_tag="VFB53_01055"
     CDS             11114..12034
                     /locus_tag="VFB53_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN85348.1"
                     /translation="MRYEDVAVLIPCLNEERTIGKVVRDFSAALPGARILVFDNASSD
                     GTALAAQRSGAAVIHAHRRGKGNVVKQMFEQVDAALYVLVDGDDTYPAPDAQRLIAEF
                     RKGGVDMVVGARIAPDGVAAYRRFHRFGNRLISALIARLFSIEVTDVLSGYRVLSREF
                     VKSVPLASSGFEIETELTLQAAAKGLASRELPIEYRPRPHGSVSKLNTFADGLVVLKA
                     IVTIFKDYKPLLFFSGLSVALALASLAVGARAILGYLDTGLVHHLPSALLATGTAILA
                     ALSLCIGLILDTLAKYHNENIQLFRRLMKK"
     gene            12038..13192
                     /locus_tag="VFB53_01060"
     CDS             12038..13192
                     /locus_tag="VFB53_01060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN85349.1"
                     /translation="MRLLFVSHSSPAKEGGAETRTREVALRLARAGHQVTVLCGRTHV
                     DDPAELDLEGVRILTCKTLPDVLLRRFPYPHYLSLAAANLFLMLHLPAFLRRERFDLL
                     REDVAPFPPTFLLALVKLPVTRRIAVTHMLPGSLRAWIGYYGALFGPAGWLMDRLLRA
                     GALRYERVVCDSKWFADELARSPALAQKVRYVPNGVDFAAFAGGGARGRGDGQVRLLC
                     VGRLAETKGHRYAIEALARLQDRHPRLHLDIVGNGALRDELVALAQRRGVVDRVKILP
                     PVAHAEMPSLYRRYDLFVMPSLWEGQPVSLIEAMASGLPIVATDIPAIRAMLDERSAT
                     LARKADVDDLAERLDWAIRNRDTVLRRAEAARTQAREYDWAATARQEIED"
     gene            13192..14853
                     /locus_tag="VFB53_01065"
     CDS             13192..14853
                     /locus_tag="VFB53_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85350.1"
                     /translation="MNQIFYASMIGTGAAFLIIAAVRASLIGPFTASFTFLVGGIDYY
                     ACLPYAAALAVALLRPVQRAGLALADACGRHPYAAAGLTAAALAIGTHLLYHAHPLSM
                     DEYAPYFQSTIFAAGEMHGRLPAPLLDWLVPKGFQRMFFTVDRATGAIVSIYYPGMAL
                     LLTPFTALGVPWLLNPLLGGATVLVMHRLGRTLLGSERAAGLAALFTIASPAVTLNAV
                     SFYSMPAHLLANALFALLLLRPTPRRAFAAGVTGSIALVLSNPVPHLFFALPWIAWLA
                     ADRARRRLLVPLIAGYVPLCGVIGLGWTYYLRGFTTSLAQGAAEPLSLAARMFSGVLQ
                     VPTKVVLEARAVALAKLWLWAAPALLVLAGLGAWRLRNDSGPWLALAGSALLTFLGYL
                     FVPYDQGHGWGYRYFHSAWSVLPLLAVAALLPAGGVRPDGVPGDPSSTGPSAAGYAAA
                     CALAGLLSVTVLLAVQQEHFIRRHLQQVPQPQPGGQARVLIIDASRPFYRIDLVQNDP
                     FLRNPVLILVSHGRAADEAMMRERFPELSLLSSSERGTVWGIRRR"
     gene            complement(14806..16380)
                     /locus_tag="VFB53_01070"
     CDS             complement(14806..16380)
                     /locus_tag="VFB53_01070"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN85351.1"
                     /translation="MSAPRVVIIGAGPAGLTAAHELAENGVRDIVVLEASAAIGGLSK
                     TVNYRGNRIDIGGHRFFSKSDWVMDWWLRMLPLEQGAAQGFDIGYQGKLRRVAAPGTP
                     ASGERVMLVRNRLSRIYFNGRFFDYPLKVNFATARKLGLVRCLLFGISYLKAKLLPRR
                     PERSLEDFFINRFGRRLYLQFFKSYTEKVWGTPCTAISAEWGAQRVKSLSIGSALRHA
                     LRRLLGGGRGAAEQTSLIERFLYPKYGPGLMWETTAEVLRARGVRIVMQSPVTRLETG
                     GGRVRAVTARRADGSEQRYEADAFISTMPVRDLVLALEPRAPERVREIAGALEYRDFI
                     TVGLLYRKLTPKSMPDNWIYIQEPGVRVGRLQIFNNWSPYMVADADTVWIGMEYFCRE
                     GDELWSMNDAALLELGKDEMRKLRLAEPADFLDGVALRSAKAYPGYYGAYANFDEIRA
                     WLDPIPNLFLAGRNGMHRYNNQDHSMLSAKLAVEAILAGSTDKSPLWQVNIDDEYHEE
                     ASAAPDSPDRAALARG"
BASE COUNT         2476 a         5901 c         5684 g         2407 t
ORIGIN      
        1 agttcaacct gctggcgcgc acgccggcag ttgaaaatgt cgagctgcct ttggtttatg
       61 ccggaattgc caagcgcgag cggcttgaaa aagccatgaa aatgctcaag caggtcgggc
      121 tcgcggaccg ggccgagcac cacccggcgc agctctcggg cggccagcag caacgcgtgg
      181 cgatcgcgcg cgcgctggtc accgagccgc tgctgatcct cgccgacgag ccgaccggcg
      241 ccctcgactc gcgcaccagc ctcgagatca tggcgctgct gcagaacctc aaccgccagg
      301 gcatgacggt ggtgctggtg acgcacgagc ccgacgtggc gcgcttcgcg caccgcattc
      361 tcggcttccg cgacgggaaa gtggtgcgcg acgagccgaa ccagcccgcc gacgccagcg
      421 agcacctgca cctggaagcc gcatgacgct gctgcgcatc gcgctgcggg ccctcgcggt
      481 caacaagctg cgcagcgcgc tcaccatgct gggcatcgtc atcggcgtcg gcgcggtgat
      541 cgtgatgatc gcggtcggct ccggcgcgca ggagcgcgtc gaggagcaga tccgggcgct
      601 gggctcgaac ctgctgctcg tcatgccggg ctccaccacc gccggcggcg tgcgcatggg
      661 cttcggctcg ggctcgaccc tcaccgagga cgacgtcgtg gcgctcaatc gcgacattcc
      721 cgaggcgctc gccgcgcccg cgctgcgcgg caccgcgcag gcggtctggg gcaactcgaa
      781 ctggtcgacg cagatcttcg gcatcacgcc cgactacctc gacgtgcgcc agtggccgct
      841 ggtttccggg cgggtgttcg agcccgcgga aatggccggc gcggccaagg tctgcctgat
      901 cggcgcgacc gtcgcccggc agctgttcgg cgacgccgac ccgctcgacc agtcggtccg
      961 catcaagcgc gtgcccttca ccgtgatcgg cgtgctcgac gtcaagggac agagcatgat
     1021 gggcaccgac caggacgatc tgatcctggt gccgatcaag accgcgcgca gccgcgtgct
     1081 cggaaccgcg agcgccgcgc gcaaccgcac cgtaggcacc atttggatca aggcggccga
     1141 cggcgtcgac accaggcgca tcgaggagca ggtgcggtcg ctgctgcgcc agcgccaccg
     1201 cctgccgatg ggcgccgacg acgatttctc gctgcgcaac ctcgccgagg tgatggcggc
     1261 gcaggaagcc tcgagccgcg tgctcgcgct gctgctcgcc gcggtggcct cggtgtcgct
     1321 gctggtgggc ggcatcggca tcatgaacat catgctggtg tcggtgaccg agcgcacgcg
     1381 cgagatcggc ctgcgcatgg cggtcggcgc gcgcacgcgc gacatcctcg ggcaattcct
     1441 ggtggaagcg gtgacgctgt ctctgatcgg cgggctgatc ggcgtcgccc tcggcatcgg
     1501 cggctcggtg gcgatcgcgc agctcgccgg ctggcgcatc gcgctctccg ccgaggcgat
     1561 cgtgctggcg gtcgccttcg ccttcgtcat cggcgtgttc ttcggcttct atccggcgcg
     1621 caaggccgcg cgcctgaacc cggtcgaggc gctgcgcttc gaatgaggcg acttccgcgc
     1681 cggtccttgc aaacgtgacg atcgccgagt tcgtctacac cactttgctc aagccgcgcc
     1741 tgttgcgtcg cgcggcgaac gccgcgatcc ggttatcgct acccgagcgc gcccgcgtcg
     1801 gcggcgcgat ggtatggctc aatccgcagg acccggtggt ttccggcgcg ctgacgttcg
     1861 gcgtgtacga gcgcggcgag atcgccttct tccgttcgcg ctttcgcgcc gacatgaccc
     1921 tcgtcgacgt cggcgcgaac gtcggcctct acagcgcgct cgcgctatcg acaccggggt
     1981 tccgcggacg cgtgctggcg atcgaaccgc acagcgagag ccgtgcctac ctgcacaaga
     2041 cgatagagag caacgccgcg caggcgccgc ctggcgccgc gctgatctgc ggccttgcgg
     2101 cttcggaccg cgcggaaacg gtgacgcttt accagaatcc cgagaacaag ggcgataatc
     2161 ggctgtatcc ggatccattg ctgcgcggcg cggagaccgt cgacgccgat accgtggaca
     2221 atatctgccg ccgtcacggc atcgcctcgg cccagttcat caagatcgac gtgcagggcg
     2281 ccgaggccaa ggtggttcgc ggcgcgaccg agctgctcgc cgccagtcgc gactgcatcc
     2341 tgatgacgga gttctggccc tatggcctgt cacgctgcgg aacggacgga ctcgcctatc
     2401 ttgaaatcct gcaggacctc ggcttgcggc tctaccagct ttccggcagc cgcggcgtgc
     2461 tcgcgccgct cagcagtccg cgcgcgctgg tcgagcgcac gcacggccgc cgctatgcca
     2521 acctggtggc tgtgaaaggc aatctcgtcg aggcgctgcg cctcgaatga gggttctcgt
     2581 gaccggcggc gcgggctata tcggctcgca catggtgcag ctgctgctcg agcgcggcgc
     2641 cgaggtcacg gtgctcgacg acctctcgac cgggcacgcc gacgccgtgc gcggcggcca
     2701 cttcgtgcgc ggcgacatcg ccgaccggca cgcgaccgga actctgctgc gggaacgcgg
     2761 catcgaggcg gtgatccatt tcgccgccgc cgccctggtc ggcgagtcga cggccgaccc
     2821 gcccaagtat tacgcgcgca acgtcggcgg cacggccgcc ctgctgcagg cgatgcgcga
     2881 ggccggcgtc gcgcgcatgg tgttttcctc cacctgcgcg atctacggca acccgcagcg
     2941 cacgccgatc gacgaggcgc acccggcgca gccggtgaac ccctacggca gatcgaagct
     3001 cgcggtcgag ggcatgctcg ccgagtgcgc cgccgcctac ggcctgcgcg cggcgagcct
     3061 gcgctatttc aacgccgccg gcgccgatcc ccaggcgcgc ctcggcgagc gccacgagcc
     3121 ggagacgcac ctgattccgc tcgtgctgca ggcggcgtcg ggacggcggc cggaggtctc
     3181 ggtctacggt aacgactggc cgacccgcga cggcacctgc gtgcgcgact acatccatgt
     3241 cgacgatctt tgcgtggcgc acctgctggc gctggaatgg ctggcgagcg aaggccgctg
     3301 cgaggccttc aacctcggca atggcgaggg cgccacggtg ctcgaagtca tcgcggcggc
     3361 gaagcgcgtc accggccggg atttcgcggt ccggttcggc gcgcggcgcg cgggcgaccc
     3421 gccgtccctg gtcgcggatg ccgccaaggc gcggcgcgtg ctcggatggg agccaaggca
     3481 cccggcgatc gacggcatcg tgcgcgacgc ctggaacttc gagcgccggc gcccgccggc
     3541 aagctagaac ctttcggtca tcagcagctt caccggctgc gcggtgacgc ggtcgatcag
     3601 caccgcgacc caaccgcgcc gcccgcgcaa ccgcagatag cgcaggctgt ccgcctggga
     3661 cccggagctc gccagccagg catcgatcac tttcgccacc tcaggctcga ccttgtgcac
     3721 ctgcgcgagg ggcaatgcct gggagaggag ctcggtcctg ccctgctcat acggcgcgta
     3781 gtgcttggga aaatgctgca ggtcctcgag gtggatcacg gcgtcgatca gccgctgctg
     3841 gtccgccgcc tcggtcggcc actcgccgaa cacgagctgc ggcccggccc acgacggctg
     3901 gcggaattgc ggataacgcg cctcggcgag ccgctccggc gccagctcgg agaccgtgac
     3961 gacctggaac tggtccttga cgaaaacgat gaacgccggg cgcgccaggg aaatgacgtg
     4021 gcagccatag accagcgcgg cgagctggca agccgcgatc accgccagat cgaacttgag
     4081 cccgggcttg ccgctcttga acacgatcag cgtcagcgcc ggccccatga cgacgtcgac
     4141 gccgatcagg atcagcgcga gcccggagcc gcccatcacc ccgaacagcg gcggcggata
     4201 ccagtacgcc agcatcagcg cgagcacgcc ggcggcgatc gcggcgctga tcgccaggtg
     4261 cgtgcctgcg gcgcgccagc gactcatcat tcctgcgcgc cattttacaa tgtgggaccc
     4321 gggggtccgc gagcagcgct agaggcgcgc ccaggaagcc ggcacgatct ccggtgggtc
     4381 cgcgcgcgcc atccagcgcg cgggcgcgac cacgatcttg ccggcctgcg agcaaagcca
     4441 tgccccccac cagctgaagg agctgttggc aatcacgtgg tggcggcaca ggctgatcag
     4501 gtgcatgtcg tggaaatttc cctccggccc gttgccttcg acgaaatggt gccggtgcgg
     4561 gatcgcaagc gcggcgcgcg cccatgccag gtcgtccgaa aagacgaaga actgcgcgcc
     4621 cggcgcgcgc tcggcgacgt gcgccatcgc ccgcgcgtag taatcgggcg tgcacaggcc
     4681 gtggaccgcc tgcgtcgccg ggtcggtgta gtccgcgcgc cgcacgtgca cgctgaccgc
     4741 gatcgagtcg ccgatgcgct gcgcccacgc gcggttggcg ccgtcgagcg ccgggaagcg
     4801 gaaatcgcgc cgtacctcgc cggccgccgc ggcaaaatag ctctccgatt gccagtagcc
     4861 gagcaggtag caggtcgccg gcgcgccggc gacggtgagc gcaggcgcct gacgcaggtc
     4921 gtccaccagc agccgcgcgc cgcgcaggcc ggagaatgcc ggccggcgca ggaccccgag
     4981 cacccgcacc gggcggcgcc agccgagcac ggcgcgcagc tcggcggcgc gcgccttctc
     5041 gaccggcaag gcgaaggcct tgcgcagcgc gaaggcatgc aggcggtagt agccgaacca
     5101 gcgtgcgtcg agcctcagcg cgacgccgtg gcgcagggcc agcgcccggc cgaaggcgta
     5161 ctggaacatc tggttgccca ggccgccgac gaggttggtg atgatcatgg cacggcgcta
     5221 ggtgcgcgcc cacgcctccc agacgaaggg gtagcaccac tcctcgcgat gggtgcgccc
     5281 gagcgccgcg cggatttcgc gccgccgcgc cgccgcgtcc gggacgaaat tgtgaaactg
     5341 caccagcagg cgcttcaccc gctcgatgcc gccgcgcgcc agcgccggaa gcacctcgta
     5401 ttcgccgccc tcgatgttga ccttcatcag gtcgatgtcg cccggcagag ccgccagcac
     5461 ctcggcgacg tccttcttct cgcagcggac cgcggcaccc ccgcgccggg cgagcgcgcc
     5521 gctcgcgtcg gcgcattcgt agaacgtgac cgggccggat gcgtcggaca gcgcccaggg
     5581 aaagatccgg atgcccgcct cgcccgcgaa gcgcgtgcgc aactcggcca gaaaagcggg
     5641 catcggctcg aaaatatcga tgcgacagcc gtagcggcga cggatctcgt gcgcccagtc
     5701 gccgcggaac ccgccgacat cgaacaccag gctgtcgcgc gtgagcggaa actcgaggcg
     5761 cagcgtatcg tcgccccggt cgcggtgcca gcgctgcacg gcgagcagga acggatcccg
     5821 caggacgtgc gcacgaaagc gccgcacgat gcgctcgagc agcgtatcca tcagcgcgcc
     5881 agcaccgcca gcagcacgcc gagacaaatc gcgagcggcg cggcgctcgc ggcaaggtcg
     5941 aagcgggcgg ccagtccgaa cgcgcggtag ctctgccgca tgctgaaggg cgtatagaag
     6001 ccggcgtacg ccgcgagcat gccgagcggc agtccggcca cgccaagcca gcggtaggcg
     6061 agcgccatgg cggcgatcat gaccagcccg cagacgccgt tggcgacgtg cacggccacg
     6121 cggttggtcg tgccgtagag ctccaggtgc agggtaccga tgcgctccac caggaacgcg
     6181 gcgccgagca gccaccacac ctcgcctgga acgaacgcgg tgcggccgcc gatcgatgcg
     6241 agcagcccct cgcccgccac gcccagcgcc gccaccccgc cgaggaacag ccagttcccc
     6301 cagagcatgc cgcgcccggc gagcgccacc acctcggcgc gccgcccatc cgcatacagg
     6361 cgcgcaagcg ccggcaagcg cgcggaaagc gggacgctcg agactgcccc catctcctgc
     6421 atcagccgca gcgcgagcag ataggaggct acctctgccg ccgctgcgag ctgtgcgtag
     6481 aacacgccgc tgccctggac cacgccgtag gtgatcgacg cgcccaggcc gctgcgccag
     6541 gcggcgggga agaccgtgtg catgacggcg ggctgcgcgg atgccgggcc gtcccacgcc
     6601 ccggccggcg cgtggcgccg cgccagggcg cggttcagcg cgtacccggc gaccgcggcc
     6661 gcgaggtgga tgcacgcgag gccggcgaga cccccgccgg cgagcagcac ggcgcacgag
     6721 accgcgaggc tcgacaggcc gaccgcgatc tccgcgcggc gcagcaccgc ggtgcgctcg
     6781 ctgccgtgca ggaaagccca gtacttcagc gagcgcagcc agatcacggt cgcgcaagcc
     6841 atcatcgccc aggcaagcca cgcgtgcgac ggctcgcgca gctgcgcgat cgggttccag
     6901 agcgcaaagg ttcccggtac tgccgcgaga aacgcgccgg cgagagccac cctcgcgtac
     6961 accgagcgca tcgtgcggat cactgcgtcc attcccgggc tgccggcgcc ggcgttggca
     7021 tacgccaccg cgcgcacgaa ggtcggcgcg aagccgaaat ccaccaaggc ggccagggtc
     7081 tgcatgctga agaacaggaa ccagaccgcg acctcctccg caggcagcag cgccagcgtg
     7141 agcggcagca gcgtcacgaa cgacagcgcg cgcgcccccc aggcgcccca ggtcatgaat
     7201 accggggaat gccagacgct catccccagg acacgcgcag ctgccagcat cacggcatta
     7261 tgagtgatcc tgctaacatc gcgcccgtgc ctgagccgct gctcaccgtc gttctgccgg
     7321 tgcgaaacgc ggcggcgtac ctgcagcgcg cgctcgacag cctgcagacc cagaccttcg
     7381 gcgacttccg cgtgctcgcc atcgacgacg gctcgaccga tggcagcggc gagatcctgc
     7441 agagcctgcg cgacccgcgc ttcagcgtca tccggcgcga agcgcgcggc ctggtgcata
     7501 cgctgaacga aggcctggaa cgcgcagcca cgccctacgt cgcgcgcatg gacgcggacg
     7561 actgctcgct gccggaacgg ttcgcgcgcc aggtgcaggc gctcgaggcc gatccggcgc
     7621 tcgcgctggt cggcagccag gcgcgcgtga tcgacgccgc gggggcgccg atggggcagc
     7681 tccctgccct taccgagcat gccgccatcc gccgcgcgct cgccgtcacc aactgcttcg
     7741 tgcacggctc gacggtgttt cggcgcgaaa tcgcgctgcg cgccggggcc taccgggcga
     7801 gcgccgcgct ggtcgaggac tacgacctgt ggcagcgcat tgccgagctg ggacgcgtcg
     7861 ccaaccacgc cgaggtgctg tacgaatggc gcgcgacgcc cgccggcgcc agccgcagcc
     7921 ggcgcgccga gcagcgcgcc gccgccgagc gcgtcgccga cggcatctgg caggcctggt
     7981 tcggcgaggc gggaccggcg ccgcgcgagg cctggccgga aatctggcgg ccgggctccg
     8041 agccgctgtt cgccagcaac ctgcatctcc tgtttgcccg cggctacttg agacgcggca
     8101 ggcgagcgct cgccttcagc cacgtgcgcg ctgcgctcgc gtttcgcccc cacaacatgg
     8161 ccggatgggc atacgccggc gctttccctc tgccaccgcg gtggtttagc gctctcgagg
     8221 gccacgcgcg taccctgatg gagcggcagc gcgggtggtg aggccgctgc cgcagccggt
     8281 ctgcctggtc cagccgaccc tgtttcccga ggcgagccgc gtcgatatct acgagtacgg
     8341 ctgcgcgctc gcccggctcg gggtcgagac gcacgtggtg gtctcggaaa accacgcggg
     8401 ccagctgccg cccggcctga tcgtgcacca gaccggcttc gcaccgcgca acacgccggc
     8461 gcgctggtgg cgcttcgccg cgtttgcccg cgcgacggtg cgtcggctga atttcggcat
     8521 cgtgcatctc ttcaatccct ccccggcgac ttatctcctc ggctggatgc tgcggcgcga
     8581 gccgcggcgg ccgtgcatca tctacgacct gcgcaccggc gggctcgggc acagcccggt
     8641 ggccatgctg gtgaacgcga tggcgcgcac cgcgccggca tttgccgacg ggctgatcgc
     8701 cgtgaccggc gcgctgggca atcacctgct cggggcgggg cgttttcgcg aagtgccgat
     8761 cggcgtcaac ctggagagct tccggccgcg cgcgcgcgcg ccgcgggctg gcgacgagtt
     8821 cgtcttcgtc tatgccggga cgctcagccg caaccgggca ctgggcaaca tgctggccgc
     8881 ctttgctcgg gtggcggagg agcatccgcg ggcgcgcctg gtgatcgccg gggatggcga
     8941 cgatcgagat cgcctggagg cattcgtcgc cgcgcatgga ttgaggcacg cggtgtcatt
     9001 tctcggcaag cgcccgcacg ccgaggttcc ggatctgctc gccgcggccg attgcggact
     9061 gtcctacgtc cccgacgtgc cgtggttcca gccgcaggcg cagctgaaga cgctcgagca
     9121 cttcgccgcc gggctcacga cggtcgccgt gcgcactgcg ggaaaccggc aatactggga
     9181 cggcctgccc gccgagctcc tcaccgcgga cgatccggag tcgtacgccg cggggatgcg
     9241 ctgcgcgctg acgggcacgt acgacccggc gcggttccgg cgcgtggcgg aagcgaactc
     9301 ctgggaccgc atcacgcggg agcggctggt tccgttctac gaagctacgc tggccgctcg
     9361 aaaaccgtga acgcgtcctc gaaatcgagg cgctgcggcg tgtctccgtc ctgccattcc
     9421 gcgaacgagt gcagcacgcg caggccgttc tgacccagca ggtcgagcag cgcctgctgc
     9481 gtcaggtcgc tgcgccagcc gccggcggcg ccggcaaccg ccccgtggtg gatcgcgccg
     9541 cgcccgcccg gacgcagcac gcgcgcgaac tccgcggcat atccccgcac ttccgcggta
     9601 ttgatatgca cgaagacatc gaagctccag agtgcgtcga tcgagcccga ttcgatgccg
     9661 ccaaggtcca ggcccgaccc gagcatgaaa gacgcgttgg cgcgcgccgc gaagcgctcg
     9721 cggcacaggt tgacgcaggc ttctgaaata tccacgccga tgaaccgggc ggagcgatcg
     9781 atcaaggcct cggtccagcg cccggagccc ggcccgatct cgagcgtggc gccgcccgcc
     9841 gccaggtggg gttcgagcag gcagcggatc accgagtcct tccattcgcc ggtgccgaac
     9901 tcgcggccct cgccgctcca gtcccaggcc tcccagatcg ccttgttgag cttcgcgctg
     9961 ttcagcccgg gcacgccgcg gctgcgccgc agattctcca tcgcggtgaa cgcgcgcccg
    10021 gcgaacgcgc tcgcgacgta gtaggcggcg aacagcacga accagcggat gccgctctcg
    10081 cgcagcgcca ttcccgccag ccgcagcttg gcgggcagcg gaatgcggcg gcgattggga
    10141 aggctatctt tcatctcggc cgggtgttcg agcgacgaag ctgaacgcgc cgctggtgct
    10201 caacacgcgc agcgcttcgg tcgcccgggg atcgaaacgt ctgccgagcg cccaccacac
    10261 gcggtgcagg agctgcagcg gcaacagcag cgatttgacc ataaccgccg gcatgcgggg
    10321 aaacagcccc atctgcgcga gcggcagcag tcccgaccag cccgccgccg ggcagacgta
    10381 gtcgaccgcg aacccggcct cctcgaggcc gctgcgcaat cccaggtggg tgtggtggta
    10441 gtagctgtcg gcgtgaaacg gctcgaggaa cgccaccgtt cccaggaaca ccgcgccggg
    10501 tcgcatgacg cggcgcgcct cccgcagagc caccgccgga aagcgcagat gctcgagcac
    10561 cgcgatcgag agcacgaagg cgaagctggc gtccttgaac ggcagcgcgt gggcatcgcc
    10621 gagaaccgaa gctccggcgg cgtcgtgatc gaggccgagg tactcgaacc ccgctgcctc
    10681 gcacacctcg cggtgcaacg cgctgccgca gccgagatcg agcatcagct cgccagttgc
    10741 cttcgcccgc ggaaagtggc tgagcagctc cggggtcagg tgatagggcg ctgcccggcc
    10801 gccaaagtcg acctggggcg acgggttccg cggcagctcg cggaattcct ccggcgccac
    10861 ggcgagcggc acgccgagcc tgaactcgtg cgccacggac ttgggttgct gcaacctgag
    10921 gtcgagcgcg ccgcttttgc tgcgagcgta gcgcgcgctg caggcgccgc acgccgcgcc
    10981 cgcgccgctg cgcacgagcg cgccgccgca gcccgggcac gccagcgcgc gccaggcggc
    11041 gtcggacagc tcgctagcca ccggcgaaaa ctccgaattg ctgcatcggt agcgagcaag
    11101 taatctgcgc tccatgaggt atgaggacgt cgcggtgctg attccgtgcc tgaacgagga
    11161 gcgcaccatc ggcaaagtgg tgcgggactt cagcgccgcg ctgccgggcg cgaggatcct
    11221 ggtgttcgac aacgcctcga gcgacggcac tgcgctcgcc gcccagcgca gcggggccgc
    11281 cgtcatccac gcccaccggc gcggcaaggg aaacgtcgtc aagcagatgt tcgagcaagt
    11341 ggacgcggcg ctctatgtcc tggtggacgg tgacgatacc tatcccgcgc ccgacgctca
    11401 acggctgatc gccgagtttc gcaagggcgg ggtcgacatg gtggtggggg cgaggatcgc
    11461 gcccgacggc gtggcggcgt atcgccgctt ccaccggttc ggcaaccgcc tgatctcggc
    11521 gctcatcgcc cgcctgttct cgatcgaggt gaccgacgtg ctgtcgggct atcgcgtgct
    11581 ttcgcgcgag ttcgtcaaga gcgtgcccct ggcgtcgagc ggcttcgaga tcgagaccga
    11641 actcaccctg caggccgcgg ccaaggggct cgcctcgcgc gagctgccga tcgagtaccg
    11701 gccgcggccg cacggcagcg tgtcgaagct gaacaccttc gccgacggcc tggtggtgct
    11761 gaaggcgatc gtcaccatct tcaaggacta caagccgctg ctgttcttct ccggcctgag
    11821 cgtggcgctg gcgctcgcca gcctcgcggt cggcgccagg gcgattctcg gctacctcga
    11881 caccggcctg gtgcatcacc tgccgtccgc gctgctcgcg accggcaccg cgatccttgc
    11941 cgcgctcagc ctttgcatcg gactgattct cgacaccctg gcgaaatacc acaacgagaa
    12001 tatccagctc ttcagacgcc tgatgaagaa atgacgcatg agactgctct tcgtcagcca
    12061 ctcgtccccg gcgaaggaag gaggggccga gacgcgcacc cgggaggtcg ccctgcgcct
    12121 cgcgcgcgcc gggcaccagg tcaccgtcct ttgcggcagg acgcacgtcg acgacccggc
    12181 ggagctcgac ctcgagggcg tgcgcatcct cacctgcaag acgctgcctg atgtgctgct
    12241 gcgccgtttc ccgtacccgc actacctctc gctcgctgcc gcgaacctgt ttctgatgct
    12301 tcacctgccg gcgttcctgc gccgggagcg cttcgacctg ctgcgcgagg acgtggcgcc
    12361 cttcccgccg acgttcctgc tggccctggt caagctgccg gtcacccggc gcatcgcggt
    12421 cacgcacatg cttcccggaa gcctgcgcgc ctggatcggc tattacggcg ccctgttcgg
    12481 cccggccggc tggctgatgg accggctgct gcgcgcgggc gcgctgcgct acgagcgcgt
    12541 ggtatgcgat tcgaaatggt ttgccgacga actggcgcgc tccccggcgc tggcgcaaaa
    12601 ggtgcgctac gtgcccaacg gcgtggactt cgccgcgttc gcggggggcg gcgcaagggg
    12661 acgcggcgac gggcaggtcc ggctgctctg cgtcgggcgg ctggcggaaa ccaagggcca
    12721 ccgctacgcg atcgaagccc tcgcccgcct gcaagaccgc cacccgcgcc tgcacctcga
    12781 catcgtgggc aacggcgcgc tgcgcgacga gctggttgcg ctcgcgcaac gccggggcgt
    12841 ggtggatcgc gtgaaaatcc tgccccccgt ggcgcacgcg gaaatgccgt cgctctaccg
    12901 ccgctacgac ctgttcgtca tgccgtcgct ctgggaaggc cagccggtct cgctgatcga
    12961 ggccatggcg agcgggctgc cgattgtcgc caccgacatc ccggccatcc gcgcgatgct
    13021 ggacgagcgc tcggccacgc tggcgaggaa ggcagacgtg gacgatctgg ccgagcgcct
    13081 ggactgggcg atccgcaacc gcgacaccgt gctgcggcgc gccgaggcgg cgcgaacgca
    13141 ggcccgggaa tacgactggg cagcgaccgc gcgacaggaa attgaagact gatgaaccag
    13201 atcttctacg cctcgatgat cggaacgggc gccgcattcc tgataatcgc cgccgtgcgc
    13261 gcctcgctca tcggcccgtt caccgcctcg ttcactttcc tggtgggagg gatcgactac
    13321 tacgcctgcc tgccgtacgc agcggcgctg gcggtcgcgc tgttgcgacc ggtgcagcgc
    13381 gccgggttgg cgctcgccga cgcgtgcggg cgccacccct acgccgcggc ggggctgacc
    13441 gccgccgcgc tcgcgatcgg gacgcacctc ctgtaccacg cgcacccgct ctcgatggac
    13501 gagtacgcgc cctatttcca gagcaccatc ttcgcggcgg gggaaatgca cggccgtctg
    13561 cccgcgcccc ttctcgactg gctggtgccg aaggggtttc agcggatgtt cttcacggtc
    13621 gaccgcgcga ccggcgccat cgtctcgatc tattaccccg gcatggcgct gctgctgacg
    13681 ccgtttaccg cgctcggcgt gccgtggctg ctcaacccgt tgctcggtgg cgccaccgtg
    13741 ctcgtgatgc acaggctcgg ccgcacgctc ctcggcagcg agcgcgcggc cggactggcg
    13801 gcgctcttca ccatcgcctc cccggcggtc acgctgaacg cggtgtcgtt ctattcgatg
    13861 cccgcgcacc tgctggccaa cgcgctgttc gcgctgctcc tgctgcggcc gacgccgcgg
    13921 cgcgccttcg ccgccggcgt gaccggctcg atcgcgctgg tgctgagcaa tcccgtgccg
    13981 cacctgttct tcgccctgcc gtggattgcg tggcttgccg cagaccgcgc acggcgccgc
    14041 ctgctggttc cgctgatcgc cggctacgtg ccgctgtgcg gcgttatcgg gctgggctgg
    14101 acctactatc tgcgcggctt caccacctct ctcgcccagg gcgcagccga gccgctgtcg
    14161 ctcgccgcgc gcatgttcag cggcgtgctg caggtgccga cgaaagtggt tctcgaggca
    14221 agggccgtgg cgctcgccaa gctatggctc tgggccgcgc cggcgctgct cgtgctcgcg
    14281 ggcctgggcg catggcggct gcgcaacgat tccggcccgt ggctcgcgct cgcgggaagc
    14341 gcgttgctca ccttcctcgg ctaccttttc gtgccgtacg accaggggca cggatggggc
    14401 taccgctact tccattccgc gtggtcggtg ctgccgctgc tcgcggtggc ggcgctgctg
    14461 ccggccgggg gggtcagacc ggacggcgtc ccgggcgacc cgtcgtccac cggaccttcc
    14521 gccgccggct acgccgccgc ctgcgcgctc gccgggctgc tttccgtgac cgtcctgctg
    14581 gccgtgcagc aggaacactt catccgccgg cacttgcagc aggttccgca gccgcagccg
    14641 ggcggccagg cgcgggtgct gatcatcgat gcctcgcgcc cgttttatcg gatcgatctg
    14701 gtgcagaacg acccgttcct gcgcaacccg gtgctgatcc tcgtgtcgca cggccgcgcc
    14761 gccgacgaag cgatgatgcg cgagcgcttt ccggagcttt cgctcctatc ctcgagcgag
    14821 cgcggcacgg tctggggaat ccggcgccgc tgacgcttcc tcgtggtact cgtcgtcgat
    14881 gttgacctgc cagaggggtg acttgtcggt actgccggcg agaatcgctt ccacggcgag
    14941 cttcgccgac agcatcgagt ggtcctggtt gttgtaccgg tgcatgccgt tgcgcccggc
    15001 caggaacagg ttggggatcg ggtccagcca ggcgcggatc tcgtcgaagt tcgcgtaggc
    15061 gccgtagtag ccgggatagg ccttggccga gcgcagcgcc accccgtcga ggaagtcggc
    15121 cggctcggcg agccgcagct tgcgcatttc gtccttgccg agctcgagca gcgccgcgtc
    15181 gttcatgctc catagctcgt cgccctcgcg gcagaagtac tccatgccga tccagaccgt
    15241 gtcggcgtcc gccaccatgt aggggctcca gttgttgaag atctgcaggc ggcccaccct
    15301 gacgcccggt tcctggatgt agatccagtt gtccggcatc gacttcggcg tgagcttgcg
    15361 gtaaaggagg ccgacggtaa tgaagtcgcg gtactcgagc gcgccggcga tctcgcgcac
    15421 gcgctccggc gcgcgcggct cgagcgcgag gaccaggtcg cgcaccggca tggtggagat
    15481 gaaggcgtcg gcctcgtagc gctgctcgga accgtcggcg cggcgggcgg tcacggcgcg
    15541 cacgcggccg ccgccggtct ccagccgggt gaccggcgac tgcatcacga tccggacgcc
    15601 gcgcgcgcgc aggacctcgg cggtcgtctc ccacatcagg ccggggccgt acttcgggta
    15661 gaggaagcgc tcgatgagcg aggtctgctc ggcggcgccc cggccgcccc cgagcagccg
    15721 gcgcagcgcg tgccgcagcg cgctgccgat cgacaggctt ttcacgcgct gcgcgcccca
    15781 ttcggcgctg atcgcggtgc acggggtgcc ccacactttt tccgtgtacg acttgaagaa
    15841 ctgcaggtac aggcggcggc cgaagcggtt gatgaagaag tcctccaggc tgcgctccgg
    15901 gcgccgcggc aggagcttcg ccttcaggta gctgatgccg aacagcaggc agcgcaccag
    15961 gccgagcttg cgcgcggtcg cgaaattcac cttgagggga tagtcgaaga agcggccgtt
    16021 gaagtagatg cgcgacagcc ggttgcgcac cagcatgaca cgttcgccgc tcgcgggcgt
    16081 cccgggcgca gccacgcggc gcagcttgcc ctgatagccg atgtcgaacc cctgcgccgc
    16141 gccctgttcg agcggcagca tgcgcagcca ccagtccatc acccagtcgg acttggagaa
    16201 gaagcggtgc ccgccgatgt cgatacggtt gccgcggtag ttcaccgtct tcgacaggcc
    16261 gccgatcgcg gcgcttgcct ccaggaccac gatgtcgcgc acgccgttct cggcgagctc
    16321 gtgcgcggcg gtcaggccgg cggggccggc gccgatgatc accacgcgcg gcgcgctcac
    16381 gccccgctcc ccgggcgcgt gaacaggagc agcttgcgcg ccgaaaagtt gaaggcgaac
    16441 accagtcccg ccgagacccc cttcgccg
//
LOCUS       DASRIE010000025        37021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2895632, whole genome shotgun sequence.
ACCESSION   DASRIE010000025 DASRIE010000000
VERSION     DASRIE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 37021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895632"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFB53_01075"
     CDS             complement(<1..958)
                     /locus_tag="VFB53_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN85352.1"
                     /translation="MKPLLALLLSLPLAAFAADKPVDAGPYVPSPQSVVADMLKLADI
                     RSTDFVVDLGSGDGRIVLTAAKVFGASGFGVEIKDELVKLSNEAARREGVEDRVKFIK
                     ADLFKTDISKATVLTMYLLPNTVNLLKDKLLTELRPGTKVLSHDYPLTGWIPEKYVQM
                     DLEDKVAISGVTTTLIYLYMVPAKVAGRWSAALPVAAGQGQLSLRLKQQVTRVSGSGQ
                     LAGKPVELEDLKLHGDRLSFKLAGTRLAFAGQVKGDAIEGEVDWAGKKSPWSAKMPPA
                     VSRAPATLQLKQQLTRVSGSARLDGRDIPLEDVRLRGDRISFK"
     gene            complement(955..2643)
                     /gene="recJ"
                     /locus_tag="VFB53_01080"
     CDS             complement(955..2643)
                     /gene="recJ"
                     /locus_tag="VFB53_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZN85353.1"
                     /translation="MRLVERPYREEDRRALVAAGLHPVLARVYAGRRIHSAAELDDDF
                     KRLLPPDSLTHCARAAALLADSIATGKKLLIVADYDADGATACAVGLRALRAMGASVD
                     YLVPNRFELGYGLTPELVDLAAARKPGLLITVDNGIASVEGVERARALGIGTLITDHH
                     LPGAELPRAECIVNPNQPGCGFASKAIAGAGVMFYVMLALRAELRRRGAFAGGDEPNL
                     AALTDLVALGTVADVVPLDANNRILVAQGLKRLRAGQAQPGLDALLRAGGREAARASA
                     FDLGFVLGPRLNAAGRLADMGLGIECLATDDAARAANCALELDRLNRERRRIEAEMLE
                     QARAAADAVADAPGAAASFFDPAWHQGVVGILASRLRERLHRPVICFARANDGELRGS
                     GRSIPGFHLRDCLDLVAKRAPALILRFGGHAQAAGLSLREADLERFREAFAGAAAQTM
                     PAEALTRTVQTDGDLEPGQMSLALAQLLEERIWGQGFPQPLFCATFTLENQRVVGAKH
                     SKLKLLKDGRRLEAMRFNSLEPLPARFRAAFRLGVNEYNGLQSMQLNLEHVETP"
     gene            complement(2630..3301)
                     /locus_tag="VFB53_01085"
     CDS             complement(2630..3301)
                     /locus_tag="VFB53_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85354.1"
                     /translation="MRDRLVLVPVTGLARAEAEALVDTLNRHFAGLHEFRVAQPDAWV
                     LRTAPAALATLSAAEMAGRDIDPQLPGGKWPALLNEIQMALHEHPVNEARELPVNSVW
                     LWGAGELPAKAAGPWRSVTAGEPVALGLARLAGIPGRAPARSAGEWLAGLPADGRHLV
                     LLEAEHALLEERWCAPLLAALKSGRIGMLSLHAPEAGLAFEIARGDLRRFWRRARTVA
                     SHAPR"
     gene            complement(3511..4905)
                     /locus_tag="VFB53_01090"
     CDS             complement(3511..4905)
                     /locus_tag="VFB53_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZN85355.1"
                     /translation="MQLLDAPLAIVDLETTGAHPAHDRVTEIAVIEVDGGEVAGEWST
                     LVNPGTSIPPAIQALTGITNDMVAEAPSFGALAAQLHERLAGRVLVAHNARFDYGFLR
                     QEFERAGLPYRARTLCTVKLSRRLYPAQARHNLDSVMQRHGIECRARHRAMGDADAVW
                     QFLRLAAAEHGEEVVAVAARQVAKQPTLPAHLDRAKVDAVPESPGVYLFYGEGAAPLY
                     IGKSVDMRARVLQHFGDDLRSPREMQMAREVRRIEWRRCAGELGALLLEARLVKELAP
                     AFNRQLRRAAQLCAFSFDGKKLRLAEDFGLEHLAQLRGLFRTRRAALEALRGLADEHG
                     LCLQSLGFEPARKGACFRRQIARCAGLCAGKEGVQAHHARLAAALAALPALAWPWRGP
                     VGVVEEDRARDAAELHVIDRWCYLGTARSEDEVPALLEQSRRLAFDPDQYKLLARYLG
                     TKRARVRELPCTPS"
     gene            complement(4905..5855)
                     /locus_tag="VFB53_01095"
     CDS             complement(4905..5855)
                     /locus_tag="VFB53_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85356.1"
                     /translation="MRIAVALLVAVFACAAQAQYPNRPVKLIVPFPPAGATDIVGRIV
                     AQKLGEQLGQSVVVENRPGAGGSIGSDLVAKSSPDGYTLLIATSSTHSIGPVLQKLPY
                     DPLKDFAPITHVANVPNVLVVSPKLPVGSVAELIALAKSKPGQLNFASSGVGTIVHLN
                     AELFKMLAGVDLVHVPYKGTALSIPDVANGSIAMLFDSLASVQPHIKAGRVRPLAVNA
                     QKRSALMPEVPTLAEAGMPAFDRYTWFGMFAPAGTPAAIVDRVQGEVLAALKAPDLLE
                     RFAAAGAEAVGSTPEQFVERIKSDAAKWAQVIKAANVKVQ"
     gene            complement(5890..6495)
                     /locus_tag="VFB53_01100"
     CDS             complement(5890..6495)
                     /locus_tag="VFB53_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZN85357.1"
                     /translation="MLFLKGLAFGFVLAATVGPMWVLCLRRTLAYGALTGFVSGMGIA
                     AADGLYGAVAAFGLTAISGFLLAQAFWIGLAGGIFLAYLGVKALLSHPVADGETARPA
                     SLAQAFASTLVLTLANPPTILAFAAIFAGLGLAASADYAAAALVTLGVFLGSAAWWVL
                     LAAFAGKLRARLGPPLLRAVNVVAGFSILGFAAWQFAQLLR"
     gene            complement(6509..7108)
                     /locus_tag="VFB53_01105"
     CDS             complement(6509..7108)
                     /locus_tag="VFB53_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN85358.1"
                     /translation="MVTVYGMADSGNCYKVKLALETLALPYRWVEVDSTKGGTRTPEF
                     LARNPNGKVPTLQLEDGSHLPESNAILFYLADGTPLLPGDRAGRARVLQWMFFEQYSH
                     EPYVAVARFILRYLPAGTPRRAELPRLQERGHQALAVMEQHLAQAPWFAGGRYTVADI
                     ALYAYTHCAADGGFDLARYPAVAAWLARVKAQPGHVALG"
     gene            complement(7118..8044)
                     /locus_tag="VFB53_01110"
     CDS             complement(7118..8044)
                     /locus_tag="VFB53_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN85359.1"
                     /translation="MAYRILSLDGGGIRGLISTILLMRLAREKGLAGWLERAQLLAGT
                     STGALIALALARGLALEEILALYHDKAATIFDDSWLDNVKDAGTILGAQYHSDGLQRE
                     LRRLFGAARLRDLKKRVLVTAFDLDNEDPNPARRRWKPKLFHNFPGSDSDGAMPVWKA
                     GLYTTAAPTYFPTVDGYVDGGVFANNPSMCALAQSQDARIGRTAPLEEVVLLSLGTGS
                     SLIYVKGDRHDWGYAQWAQALLGVMFDGTIGIADFQCRQMLGRHYHRVAPSFAPGEAF
                     DLDSVDRIAEMVAFANQVDLAPAAKWLRRYWH"
     gene            8074..9315
                     /locus_tag="VFB53_01115"
     CDS             8074..9315
                     /locus_tag="VFB53_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZN85360.1"
                     /translation="MRYELLVGLRYTRAKRRNHFISFISLISMAGIALGVAALIVVLS
                     VMNGFQQELRQRILAVVSHVQLSGPGNRLDDWRAVAKGALEHPRVREAAPYINAQGML
                     THGQGVRGAIVRGVLPAEEERVADIGRHMRSGSLAELKPGGFGIVLGGELARALGALP
                     GDKVALIAPQGQVTPAGVIPRLKQFTVVGTFDIGMFEYDSSLALMHLADAQTLYRLGG
                     AVSGVRLKLEDLFAARTVSAQLMASLGAGVFASDWTRSHANFFRAVEIEKRVMFIILT
                     LIVAVAAFNIVSTLVMLVTDKQADIAILRTLGAAPASILQIFVVQGALIGVIGALIGV
                     AGGVLLALNVGTVVPAIESLLGFKFLAKDVYYISDLPSDVQLRDVVTIGLVSLAMSFV
                     ATLYPSWRASRVNPAEALRYE"
     gene            9308..9988
                     /locus_tag="VFB53_01120"
     CDS             9308..9988
                     /locus_tag="VFB53_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85361.1"
                     /translation="MSEAVLECRGLAKTYQGPQPVEVLRGVDFAVRARERVAIMGKSG
                     SGKSTLLHLLGGLDAPTAGAVSVQGRAFSAMGEAERGQVRNATLGFVYQFHHLLPEFT
                     AIENVAMPLLIRRMDRAQALDKARATLAEIGLAQRLQHLPGELSGGERQRAAFARALV
                     TEPACVLADEPTGNLDTHTSDEVFEAMVKLSALRGTAFVLVTHDAALAARAERVLELR
                     DGLLQQKR"
     gene            9997..11541
                     /locus_tag="VFB53_01125"
     CDS             9997..11541
                     /locus_tag="VFB53_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZN85362.1"
                     /translation="MALLQLLLFLLLCALALGWLSRRVNLPYPIALVIGGSLLGFVPG
                     LPQVEFDPAFLLVLVLPPILYQAALLTSWRDFKANLKPIGFLAIGLVIVTTLAVGAVL
                     KLLVPGIPWAVAFAFGAIVSPPDAVAATAVLSRYNIPHRVVVVLEGESLVNDASGLVL
                     YKFAVAAVLTGAFSLAEASVQFVLVSAGGIAVGMLIAWVFVQLHRHIGDPFIEVLVSL
                     MIPYGAYIAAESAHLSGVLAVVAAGLFRGRHAPKIVSAEMRILARSVWNILVFMLNSL
                     IFILIGVELRVIVGRLDGYSAGELAWTAIAVSAAAILVRFLWVYPAAHAAHAARAAPL
                     PPRGQLFLVSWCGMRGIVSLAAALALPLETFAGEPFPYRDLVVFLTFVVILVTLVGQG
                     LTLAPIIRRLGLGSEAGLRAEQLRARSDMGRAALLAIDAHAEEHGVAAPLAARVRAEF
                     AEKIAQETPAGPVEPADAGPARALRLAALRAERAELIRVWQLNHISDDVLHHLEEELD
                     YQESRL"
     gene            complement(11480..11827)
                     /locus_tag="VFB53_01130"
     CDS             complement(11480..11827)
                     /locus_tag="VFB53_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85363.1"
                     /translation="MTHAEFVDAWRGARVQVDPKGAAQLLSARLMLPFVLLPALGAAV
                     ALALVGHYFYGGALFAAALALRHAVRRSAGGYVLQRALADERFYAEALAAGLITAIPG
                     SPVLPRGGAARRR"
     gene            complement(11824..12378)
                     /locus_tag="VFB53_01135"
     CDS             complement(11824..12378)
                     /locus_tag="VFB53_01135"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZN85364.1"
                     /translation="MLPRDLFRKFLPSATSVRQNRLVGMFGSLLRHPNLWHLNRDSVA
                     GAVAIGLFAGLVPGPLQMLMALLLAVPLRRNLPVALLMTLYTNPFTIVPLYLLAYGYG
                     GLLLGGSHGEAKIEPFDMDWGHFLDSMARMLDWGLALGKPLLVGLVALGLTLAALGYV
                     AVQVGWRAYVVLAWRARQRKRQQE"
     gene            12417..14726
                     /locus_tag="VFB53_01140"
     CDS             12417..14726
                     /locus_tag="VFB53_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZN85365.1"
                     /translation="MTGAALAFTAGVLLLQQQAALPAPAWLLLLPCCAALAARRRALV
                     LPAALAIGFLWAAGCAHLRLADRLAPELEGRDLDVAGVVAGLPAASERGLRFAFDVES
                     AAARLPQRIQLAWYRNAWREDAAAAVPETLRPGERWQFTVRLRQPHGHANPHGFDYEA
                     WLTERGIGATGYVRQGAAARRLGERESLLDRIERTRAAVRDRFKSVLGETPSAGILAA
                     LAVGDQRAISSEEWRLFGRTGVTHLMSISGLHVTLVSGLAAWLVSALWRRVPRLALAL
                     PARKAGAAAAILAALAYTLLAGFAVPAQRTFYMVTVVALALWSGRIASPVRTLALALA
                     VVAALDPWAVLQPGFWLSFGAVALIFHVTAGWTQSGPRALQWLRVQWAITVGLAPAAL
                     FLFAQLSVAGPLANALAIPLVSAVITPLALAAALLPADALLLGAQGLTEWLLVALEWC
                     AQLPAAVWQQHAPPLWATLLALAGAAWLLLPRGFPWRASGLALMLPAALLPAPAPPPG
                     EAWVTVLDVGQGLAVLVRTANHALLYDAGPAYGAEADSGERLVVPALRALGVARLDAL
                     VLTHNDADHTGGAQSVLDNLEVGALRTSLPGRHPLLAMAAAPARCARGQSWDWDGVQF
                     EFLHPPPGAPARRRNDDSCVLRVAAAGAAMLLAGDIERGAEGRLLDAAALRADVLLVP
                     HHGSRSSSSEAFLDAVRPRLAVAAAGYRSRFGHPHAEVLERYAARGIGVLRTDLDGAV
                     SVRLAPGGASAAGERARRARYWHVPAPAA"
     gene            14773..14991
                     /locus_tag="VFB53_01145"
     CDS             14773..14991
                     /locus_tag="VFB53_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZN85366.1"
                     /translation="MAKGSNAVYRVTDVIGTSPNSWEEAARNAVKTAAKSLRDLRIAE
                     VTKLDVKVEDGKVTQFRTRLSLSFKYSA"
     gene            15051..15797
                     /locus_tag="VFB53_01150"
     CDS             15051..15797
                     /locus_tag="VFB53_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN85367.1"
                     /translation="MLVLLRLIRKIVGWVDFLAVCLVVYLLAWLPWPGKHPVARLFPV
                     WCRAFARALDVDIRVHQKHRRQHLPERYILIANHPSAFEDIGIPAVFDVVSVAKLQVK
                     DWFIAGRIARAAGTLFVDRDNPESRKQVIDEMVQAVSGGRNIALYPEGGCKGRRLHSE
                     FKTGAFEVSFRTGIPILPLFLHYEAQDDFEWQPPYTLPDKIRHIMTAANNRANYYVYE
                     PLDPKDYADRYAMKAAAYALYTRWNAQYLE"
     gene            15809..16129
                     /locus_tag="VFB53_01155"
     CDS             15809..16129
                     /locus_tag="VFB53_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85368.1"
                     /translation="MATAIVRVTGGVGRFEEFRERIRWLLVRDPDVDPHTEHHAPDRL
                     EYRFALVKGIPFPSFVAASGEYPELRVEVQWDKGGDQAGAKGGVAIEQGRIVEQWAGE
                     RKPG"
     gene            16126..16980
                     /locus_tag="VFB53_01160"
     CDS             16126..16980
                     /locus_tag="VFB53_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN85369.1"
                     /translation="MSVETRLRYVQCASPRGLHRIAYRESGDPQAPALVCVHGLTRCA
                     RDFDALAAAMSDRYRVICPDVAGRGDSDWLADPMLYQLPQYVADLVTLVARLDVERVH
                     WVGTSMGGLIGMALAAQKNSPVSKLVMNDAGPVIAKASLQRIGSYVGLAPAFATIEAA
                     EQYVRTVHAPFGPHSDTQWRFLTEAVVRRNADGSYRLHYDPKIGEPFRQNMPAGDLES
                     WPLWDAIRCPALVLRGAQSDLLSKATAQEMTQRGPKAKVVEFAGVGHAPTLMHADQIA
                     AVREFLLS"
     gene            complement(16982..18115)
                     /locus_tag="VFB53_01165"
     CDS             complement(16982..18115)
                     /locus_tag="VFB53_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN85370.1"
                     /translation="MDPLAAYDALTAGRTRFLPLDELCRQAAERYPGLVPGAKDLEAE
                     AALAQRDKRGLEKAQGAFLAAVLADPAAGAHLCHAMLLPRADSAKALAEFEAKGELSL
                     AGAQLARQGKAVVLTMRNPRYLNAEDETTLEGMEIAVDVALMDRKSELCVLRGDVVTH
                     TKHAGQRVFGAGINLTHLYQGKIRFLWYLIRDLGFVNKMYRGLALPGVNPEVDCREKP
                     WVAAVERFAIGGHCQILLTMDYVLAAEDAYMTLPARKEGIIPGAANLRLPRFVGARIA
                     RQAILAERRLDCASAEGRMICDEIVPAAGMDQAIARSIEHLTNSGVVSAAGNKRAFRA
                     GEEPLDTFRRYMAVYAREQAQCHFSPALIDNLERHWDAANRRG"
     gene            18205..18651
                     /locus_tag="VFB53_01170"
     CDS             18205..18651
                     /locus_tag="VFB53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN85371.1"
                     /translation="MGLYRAETYRVEDSVGYLVTRVRASIFAAVDAELADWGISAAQG
                     SILVYIGHGRGDRAADIARDYDYDTGSMTRMVARLSAKRLLRRVPDENDRRVVRLDLT
                     AAGRKLTEQMAAVAAKVLNQHLRGFSRAELEQLKALLHRMLDSARG"
     gene            18644..19765
                     /locus_tag="VFB53_01175"
     CDS             18644..19765
                     /locus_tag="VFB53_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN85372.1"
                     /translation="MASRRRALTLLILLFAGAGVAWAAWWQVVGRHYESTDNAYVAGN
                     LVEVTSQVAGTVIAIGADDTERVEAGQVLVRLDPADARMALEQAEAELARAVRDARTL
                     YATNLSLEANVRVRQAELQRAEEFVARRTALAGQGVVSREDYDNARSGAAAARAALAS
                     AREQLATNQVRTERVRVEDQPGVLGAAARVREAWLALRRTEVPAPVSGMLARRSVQVG
                     QRVAPGAPLMVVVPLEQVWVDANFKEVQLRDMRIGQPVRLSADLYGGEVQFRGKVAGL
                     AAGTGGAFALLPPQNATGNWIKVVQRLPVRIALDAEELRRHPLRIGLSMRAEVEIRDK
                     SGRQLASAPRSGTAYATEAFAASPAEAEARVRAIIAAHR"
     gene            19762..21336
                     /locus_tag="VFB53_01180"
     CDS             19762..21336
                     /locus_tag="VFB53_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZN85373.1"
                     /translation="MSAAAPAVAAPAPAPLAGAERMLGAVMLAAATFMVVLDITVANV
                     SVATIAGDLGVSATQGTWIVTSYAAAQAIAMPLTGWLTRRFGQVRLIVASVLLFTAAS
                     WLCGLAESLETLVAFRVLQGLVAGPMVPVSQSLLLATFGPGGAGMALAVWSMTATIAP
                     ITGPIIGGYITEQIAWPWIFYINVPVGLLAGGMIWLLYRERETPRQRVPVDVVGLVLL
                     TVWVAALQVMLDKGKELDWFASGEIVALACAAGLGFALFLVWELLDNPHPVVDLQLLR
                     DRDFWTATATLSVGYAVFFGAIVVLPLWLQQTMGYTQIWAGLMMMPFGVLALVLAPVI
                     ARTLHLVDARLVASLGFLVFALVSFMRAGFDMTADWMTIALPMFIQGAGNVMFFLPLT
                     ALGLRGMSADKMPGATGLQNFLRYMGGAVGASLAVTLWDDRAKLHRAHLAEHLTRGDP
                     ATEGALRALEAQGLGGAQALAHLDRALEVEARMLSTVDLFWISGVLFLALAALVWLAN
                     PPRAAAKMHAATGSHE"
     gene            21333..21974
                     /locus_tag="VFB53_01185"
     CDS             21333..21974
                     /locus_tag="VFB53_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN85374.1"
                     /translation="MKDFTPTTRTRIKRLPKRARYDRDSVYAILDAGFVCHIGYVIDG
                     HPFVTPTAYWREGDAVYWHGSSKSRMLMALQKSPKVCLTVMHLDGLVVARSGFHKSIN
                     YRSAMIFGKPYQVTEPQEKLAKMEKFVERLYPGRWPGLRAVHKQELKATTVLGMHIDE
                     AVAKVRSGGPIDDDEDYRLPIWAGVFPVRQVLGEPVDDGRLAPGAPGAPRVTL"
     gene            complement(21971..22426)
                     /locus_tag="VFB53_01190"
     CDS             complement(21971..22426)
                     /locus_tag="VFB53_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN85375.1"
                     /translation="MKTLLIVYHTGGAKTARMAEAVERGARSEEGVRVVVKRCAEAGP
                     EDLLGADGLILGTPENFGYMAGMMKDFLERVFYACEGKVEGRPWALFVGAGQDGSGAV
                     TSVERIVTGLRLKKIAEPVVVVKELKPEQLAACEELGAAMAAGIAAGIL"
     gene            complement(22423..23181)
                     /locus_tag="VFB53_01195"
     CDS             complement(22423..23181)
                     /locus_tag="VFB53_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HZN85376.1"
                     /translation="MGWLRTWRRRRLLARRRIDEALWRAVTSALPFLRGLPPQDAQRL
                     RELALLLLAEKEFSGTHGLELDDAMRLSIAVQACLPVLNLGLDRYDVWSEIVVHPGDF
                     KVKVTETDDHGVVHEFDDERAGESWRGGPVVLSWDAARNAPDMNVVIHEFAHKLDMAN
                     GEADGMPPLHAGMDRRQWQEVFRVAYQGFCDAVDRGRETWLDPYAAESPGEFFAVMSE
                     AFFASPNETRRRYPDAYEQLKRYYRQDPAMRLAT"
     gene            23217..25217
                     /locus_tag="VFB53_01200"
     CDS             23217..25217
                     /locus_tag="VFB53_01200"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZN85377.1"
                     /translation="MVSVVQLHPQNSAAALAPLAAGLAPEAQARLADALAFAEPLYAG
                     QTLSTGEPVWPHALGLGASLAAIGADPAARAAGVLFAAPKFLGAGEALAARFGAEIAG
                     LAAGVEKLYQLRVLTRTSAAQQSETLRKMLLGMVEDVRVVLIRLASRTQTLRWFARNP
                     GEERAAYARETLDIYAPLANRLGVWQLKWELEDLSFRFLEPETYQRIAKMLDERRVER
                     EQYIESAVAALARELAAGGVRAEVSGRPKHIYSIWNKMRRKGLDFGQLHDVRALRVIV
                     PEVKDCYAALGAAHNLWAPIAREFDDYISRPKDNLYQSLHTAVVGPGGKTLEVQIRTE
                     EMHRHAELGVAAHWRYKEAGRATRADSSFDDKIAWLRQLLAWRDEVADSGSWAEKTRR
                     AALDDTIYVVTPQGKVVDLPKGATPVDFAYALHTDIGHRCRGAKADGRIVPLDTPLHS
                     GQRVEITVAKAGGPSRDWLSAERGFLTTSRARAKVRHWFNLQEGGREPAAPLRPKLAP
                     PPAPLAPKKKAAAGGGILVVGMDRLMTQLAKCCKPVPPDAIRGFVTRGRGVSIHRADC
                     ASLQRLAEKDAARLIEAQWGGAPGAYGVDMVVTASDRPGLLRDIGDALARERINVTAV
                     RTQSRDELATMRFAFAIADLGQLKRAFAVVREVPGVLRVARG"
     gene            complement(25224..25604)
                     /locus_tag="VFB53_01205"
     CDS             complement(25224..25604)
                     /locus_tag="VFB53_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZN85378.1"
                     /translation="MDAKSPYKGATGMRRLLDATGYSLAGLAAAVRLESAFRQELLLA
                     AILVPLGIWLGADGVERALLAGSVLLVPVVELLNSAVEAAVDRVSLEDHELARRAKDY
                     GSAAVMLALAAAGVVWLLVLLPRL"
     gene            complement(25626..26078)
                     /locus_tag="VFB53_01210"
     CDS             complement(25626..26078)
                     /locus_tag="VFB53_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN85379.1"
                     /translation="MDLILWRHAEAAAGAPDLERGLTPRGREDAARVAAWLRERLPAE
                     FVVLASPARRAQETARALCAEPQTVPGLAPGAPLEAILDAAGWPGGGRAVVVVGHQPD
                     LGRAALRLAAGAAGGGSIAAGGLWWLRSHPRGEGAAAVVHAVLAPDLL"
     gene            26109..26185
                     /locus_tag="VFB53_01215"
     tRNA            26109..26185
                     /locus_tag="VFB53_01215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26143..26145,aa:Val,seq:gac)
     gene            26191..28092
                     /gene="thrS"
                     /locus_tag="VFB53_01220"
     CDS             26191..28092
                     /gene="thrS"
                     /locus_tag="VFB53_01220"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZN85380.1"
                     /translation="MPNIKLPDGSVKTFPGPVTVAEVAQSVGAGLARAALAGRVDGRL
                     VDTSFRIEGDAALAIVTDRDAEGLEIIRHSTAHLLAQAVKQLYPEAQVTIGPVIENGF
                     YYDFSYKRPFTPEDLAAIEKRMGEIAKQDLPVERREMPRDEAVKYFRGLGEIYKAEII
                     ASIPAKEAISLYGQGGWVDLCRGPHVPSTGKLKVFKLMRVAGAYWRGDSKNEMLQRIY
                     GTAWAKKEDQDAYLKMLEEAEKRDHRRLGTQLDLFHMEEEAPGMVFWHPKGWTLWQQV
                     EQYMRRVYRDGGYLEVRGPMVLPVSLWKITGHWENYREHMFTTESEKRDYALKPMNCP
                     GHVLIYNKGVRSYRDLPLRYGEFGSCHRNEASGALHGLMRIRGFTQDDGHIFCTQEQI
                     VPECVAYTALVQKVYAHFGFKDIVYKVATRPEKRLGTDEQWDRAEGALKLSLEEAGAR
                     YEIAAGDGVFYGPKIEYHLKDAIGRQWQCGTMQVDFQLPVRLGAEYVAADNTRQVPAM
                     LHRATIGSMERFIGILIEHYGGAFPLWLSPEQVAVLNISEHQADYAKAVAAALRQAGF
                     RVVEDLRNEKISYKIREHSLQKLPYQLVVGDKEKQAGSVAVRTRGGEDLGAMALDDFI
                     ARLRNEASA"
     gene            28128..28757
                     /gene="infC"
                     /locus_tag="VFB53_01225"
     CDS             28128..28757
                     /gene="infC"
                     /locus_tag="VFB53_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZN85381.1"
                     /translation="MSPIALERSQRINGEINAPQVRLVGEQGEQHGIVAIADALRMAE
                     EQNVDLVEIAPLAVPPVCKLMDYGKFRYREQKKAHEAKLKQKQIQVKEIKFRPGTDEG
                     DYKIKLGKLTQFLEDGDKAKVTLRFRGREMAHQEFGVRLLERVKADLEKVGLVEQFPK
                     LEGRQMVMVLAPKKKGVPPKARPKADKEPREAKEAKAKKESAAKPAAAT"
     gene            28849..29049
                     /gene="rpmI"
                     /locus_tag="VFB53_01230"
     CDS             28849..29049
                     /gene="rpmI"
                     /locus_tag="VFB53_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZN85382.1"
                     /translation="MPKVKTKKSAAKRFKIRSSGSIKRSQAYMRHILTKKSTKRKRHL
                     RGTTEVHATNKRAVRAMLPYSS"
     gene            29059..29418
                     /gene="rplT"
                     /locus_tag="VFB53_01235"
     CDS             29059..29418
                     /gene="rplT"
                     /locus_tag="VFB53_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZN85383.1"
                     /translation="MPRVKRGVTARARHKKVLKQAKGFRGRRGNVYRVAKQAVMKAGQ
                     YAYRDRRNRKRTFRALWIARINAAVRELGMSYSTFMAGLKRANIEIDRKVLADLAVAD
                     KPAFARIASQVKASLAH"
     gene            29438..30448
                     /gene="pheS"
                     /locus_tag="VFB53_01240"
     CDS             29438..30448
                     /gene="pheS"
                     /locus_tag="VFB53_01240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZN85384.1"
                     /translation="MEELESLVGSALREFGEARDAPALEDAKARYLGKSGALNAFRPA
                     GGTPEERKAAGAAYNAAKERIERALAARRAELAGAQLESRLAEEALDVTLPARGRGRG
                     GIHPIIRTWQRIEALFGSIGFAVADGPEIETDWYNFTALNNPENHPARSMQDTFYVDL
                     KDAQGLPLLLRTHTSPMQVRYARMHKPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWI
                     DEGISFADLKGVYVDFLRRFFESDDLQVRFRPSYFPFTEPSAEIDLQFASGPLQGRWL
                     ELSGAGQVHPQVVRNFGLDPERYTGFAFGSGLERLAMLRYGVDDLRLFFDGDLRFLRQ
                     FA"
     gene            30445..32802
                     /gene="pheT"
                     /locus_tag="VFB53_01245"
     CDS             30445..32802
                     /gene="pheT"
                     /locus_tag="VFB53_01245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN85385.1"
                     /translation="MNVPESWLRSFCDPSLSGRQLADKLTMAGLEVEGYAPVGAQLQG
                     DVVVAEVLSVEKHPNADKLTVCRVRAGRDTVQVVCGAPNVRPGMKAPLARVGVKAVRG
                     IESHGMLASARELGLSEDHSGILELPASAKPGSDARKLLGLDEHVMTFKLTPNRPDCL
                     SVLGIAREVSALTGAKLTAPAIRPVAAESKAKHPFGITHAEGCGRFAGRVIRGVDAAA
                     PTPGWMRQRLERAGQRSISALVDVTNYVMLELGRPLHVYDQDKLRGAIDVRWGRKGEK
                     VLLLNGEEVEVDPAVLCIADDSGPIGLAGIMGGESTKAETSTRNLFLESAFFHPEAIA
                     GRARRYNFASDASHRFERGVDFANNVDGIERATQLILEICGGEPGPTVDLVKRLPKRN
                     PVRMRASRAQKVIGIPVSEKEMGGVFKRLGLPFKAKAGRFTVAPPSFRFDLQIEEDLI
                     EEVARVHGFERIPARPPRVPAAMAALPEARRSLHALRERLAACDYQEVINFSFVEPQW
                     EADLAGEANPVRLLNPIASQSSAMRTTLLGGLLANLRYNAARKLSRIRVFEVGRVFLR
                     DPSVKDGPLEVAGLRQPMRIAAAACGPALEEQWGAVARQVDLFDLKADVEALVFPRAA
                     RFEAAAHPAFHPQRSARIRVEGREAGWIGELHPRWQQKYEINQSVIIFDVDAGVLQDI
                     ALPQASIPSRFPPVTRDIALLVNADTEVQALLDAVSEGKPAIVREVRLFDLYQGPNLP
                     AGRKSLAFRVVMQDTERTLTDAEADSALQALVELWGRRFGATPRT"
     gene            32825..33142
                     /locus_tag="VFB53_01250"
     CDS             32825..33142
                     /locus_tag="VFB53_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HZN85386.1"
                     /translation="MTLTKAELADLLFEKVGLNKREAKDMVEAFFEEIRGALERGESV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPISARRVVTFHASQKLKAMVEKARHGKPQPQ
                     QES"
     gene            33111..33503
                     /locus_tag="VFB53_01255"
     CDS             33111..33503
                     /locus_tag="VFB53_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN85387.1"
                     /translation="MESRNPSKKAELPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEF
                     TQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEEGFTISGARNRLDNALGSEERAEPAG
                     RAAAGNGTGADLSAIKRELRALLETLRA"
     gene            33556..33921
                     /locus_tag="VFB53_01260"
     CDS             33556..33921
                     /locus_tag="VFB53_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85388.1"
                     /translation="MRFTLAAEQDFLRAELFERETAAQTREFLAAAAAEALRLGLSKA
                     LISVHASRTIFRVEQYQFSAYVKALAERPSAMVALVAGSDEIHAAHQYVELLAQQHGA
                     NVRCFRHEAEAVQWLRSRI"
     gene            33931..34007
                     /locus_tag="VFB53_01265"
     tRNA            33931..34007
                     /locus_tag="VFB53_01265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33965..33967,aa:Pro,seq:ggg)
     gene            34018..34923
                     /locus_tag="VFB53_01270"
     CDS             34018..34923
                     /locus_tag="VFB53_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85389.1"
                     /translation="MLQDKVVVITGAGGGIGRDFALAFAAHGARVVVNDLGTSVAGEG
                     ASSGPAQKVVDEIKSQGGRAVASTDSVAEWESANRIVKTAIDAFGRIDCVVNNAGILR
                     DRFFFNMSVEEWKAVIDVHLNGAFYVARAAAPYFKGQNAGCYVNMTSTSGLIGNLGQA
                     NYSAAKLGIAALSKSIALDMAKYRVRSNCIAPFAWSRMIGSIPTETAEQKARVEKMKV
                     MQTAKIAPLAVYLASDAAQDVSGQIFAVRANEIFLMSQSRPVRSVHRAEGWTPQTIGA
                     QAMPALRGSFFPLDRTQDVFTWDPL"
     gene            34908..36074
                     /locus_tag="VFB53_01275"
     CDS             34908..36074
                     /locus_tag="VFB53_01275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN85390.1"
                     /translation="MGPALADAGHAELRAGVRGLCAQFDSAYWQKVDEARAYPEAFVD
                     ALTAAGWMSALIPEQYGGSGLSLTQATVIMEEISRSGGNPGCCHGQMYNMNTLLRNGS
                     EAQKRKYLPRIASGELRMQAMGVTEPSAGTDTTKIKTVAVKKGGRYVVNGQKVWTSRL
                     QHSDLMILLARTTPLAEVKKKSEGMSVFLVEKAQALGKGLSMRPIRNMVNHETNEVFL
                     DNLEIPADNLIGEEGQGFRYILDGLNAERILIAAECIGDGYWFTERAVKYAKERVVFD
                     RPIGQNQGVQFPIAECHMEVEAANLMRYKAAALYDAKQHCGAEANMAKHLAAKASWEA
                     ANVCLQTHGGFGFAAEYDIERKFRETRLYLVAPISTNLILSYVAEHVLGLPRSF"
     gene            36112..36855
                     /gene="surE"
                     /locus_tag="VFB53_01280"
     CDS             36112..36855
                     /gene="surE"
                     /locus_tag="VFB53_01280"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZN85391.1"
                     /translation="MRILVSNDDGYFSPGIALLAEQLAPLGEVTVVAPERDRSGASNS
                     LTLDRPLSVRRAPNGFLYVNGTPTDCVHLAVTGLLDELPDMVVSGINLGANMGDDTIY
                     SGTVAAAMEGFLLGIPSLAVSLASREAQHLQSAAAVARGLVERFGRAPFGQPVLLNVN
                     VPDVPPQALGAIEVTRLGRRHKAEPVEKTRTPRGETVYWIGAAGAAADAGPGTDFHAV
                     AARRVSVTPLQVDLTHHAHLGMLQDWLAR"
BASE COUNT         5445 a        13033 c        13320 g         5223 t
ORIGIN      
        1 gcttgaagct gatgcggtcg ccgcgcaggc gcacgtcctc gagcgggatg tcgcggccgt
       61 cgagccgcgc gctgcccgag acgcgcgtga gctgctgctt cagctgcagc gtcgccggcg
      121 cgcggctcac ggcgggcggc atcttcgcgc tccagggcga tttctttccg gcccaatcca
      181 cctcgccctc gatggcatcg cccttcacct gcccggcgaa ggcgagcctg gtgccggcaa
      241 gcttgaagga gagccggtcg ccgtgcagct tcaggtcctc gagctccacc ggcttgccgg
      301 cgagctgccc gctgcccgag acgcgcgtca cctgctgctt gagcctcagg ctgagctgcc
      361 cctggccggc ggccaccggc agcgccgcgc tccagcggcc ggcgaccttg gccggcacca
      421 tgtagaggta gatgagggta gtggtgacgc cgctgatcgc caccttgtcc tcgaggtcca
      481 tctgcacgta cttctcgggg atccagccgg tgagcgggta gtcgtgcgac agcaccttgg
      541 tgccgggccg cagctcggtg aggagcttgt ccttcagcag gttcaccgtg ttgggcagga
      601 ggtacatcgt gagcacggtg gccttcgaga tgtcggtctt gaacaggtcg gccttgatga
      661 acttcacccg gtcctcgacg ccttcgcgcc gcgccgcctc gttggagagc ttcaccagct
      721 cgtccttgat ctccaccccg aagccgctgg cgccgaacac cttcgccgcg gtgagcacga
      781 tgcgcccgtc gcccgagccc aggtccacca cgaaatccgt cgagcggatg tcggcgagct
      841 tcagcatgtc ggccaccacg ctctgcggcg agggcacgta ggggcccgcg tccacgggct
      901 tgtccgcggc gaacgccgcg agcggcaggg agaggaggag ggcgaggagt ggtttcatgg
      961 cgtttcgacg tgctccaggt tcagctgcat ggactgtagg ccattgtatt cgttcacgcc
     1021 gaggcggaaa gcggcgcgaa agcgcgccgg caggggctcg agggagttga agcgcatggc
     1081 ctccagccgc cggccgtcct tcaggagctt cagtttgctg tgcttcgcgc cgaccacgcg
     1141 ctggttctcc agcgtgaagg tggcgcagaa cagcggctgc ggaaagccct gcccccagat
     1201 gcgctcctcg agcagctgcg cgagcgcgag cgacatctgc cccggctcca ggtcgccgtc
     1261 ggtctgcacc gtgcgcgtga gcgcctcggc cggcatggtc tgcgcggccg cgccggcgaa
     1321 ggcctcgcgg aagcgctcga gatcggcttc gcgcaggctg agtcccgcgg cctgcgcatg
     1381 cccgccgaag cgcaggatga gggccggggc gcgcttggcg acgaggtcca ggcagtcgcg
     1441 caggtgaaac cccgggatgg accgtcccga accgcgcagt tcgccgtcgt tcgcccttgc
     1501 gaagcagatc accggccggt gcagccgctc gcgcaaacgc gaagcgagaa tcccgaccac
     1561 gccctggtgc caggcgggat cgaagaagga cgccgccgcc cccggcgcat cggccaccgc
     1621 atccgccgcc gcgcgcgcct gctccagcat ctcggcttcg atcctgcgcc gttcgcggtt
     1681 caggcgatcg agctcgagcg cgcagttcgc cgcgcgcgcc gcgtcgtcgg tcgccaggca
     1741 ctcgatcccc aggcccatgt cggcgagccg cccggcggcg ttcagccgcg gcccgagcac
     1801 gaaaccgagg tcgaaggcgc tggcgcgcgc cgcctcccgg ccgccggcgc gcaggagcgc
     1861 gtccagcccc ggctgcgcct gcccggcgcg caggcgcttc agcccctgcg cgacgagaat
     1921 gcggttgttg gcgtcgagcg gcaccacgtc ggccaccgtg ccgagcgcca ccaggtcggt
     1981 gagcgcggcg aggttgggct cgtccccgcc cgcgaacgcg ccgcgccggc gcagctccgc
     2041 gcgcagcgcg agcatgacgt agaacatcac gcccgccccg gcgatggcct tggaggcgaa
     2101 gccgcagccg ggctggttgg gattgacgat gcactcggcg cgcggcagct ccgcgcccgg
     2161 cagatggtgg tccgtgatca gcgtgccgat gccgagcgcc cgcgcgcgct ccacgccctc
     2221 gacgctggcg atgccgttgt ccacggtgat gaggaggccg ggcttcctcg ccgcggcgag
     2281 gtccaccagc tccggggtga gcccgtagcc cagctcgaag cggttcggca ccaggtagtc
     2341 cacgctcgcg cccatggcgc gcagcgcgcg caggccgacg gcgcaggcgg tggcgccgtc
     2401 ggcgtcgtag tcggcgacga tgagcagctt cttcccagtg gcgatactgt cggcgaggag
     2461 cgccgcggcg cgggcgcagt gcgtcagcga atccggcggc agcaggcgct tgaagtcgtc
     2521 gtcgagctcc gccgcggaat ggatgcgccg cccggcgtac acccgtgcca gcaccgggtg
     2581 caggcccgcc gcgacgagcg cgcggcggtc ctcttccctg taggggcgct caacgaggcg
     2641 catggctcgc caccgtgcgc gcgcgccgcc agaagcggcg caggtcgccg cgcgcgatct
     2701 cgaaggcgag gcccgcctcg ggcgcgtgca ggctcagcat gccgatgcgg ccgctcttca
     2761 gcgcggcgag cagcggcgcg caccagcgct cctccagcag cgcatgctcc gcctcgagca
     2821 gcaccaggtg gcggccgtcc gccggcaggc cggccagcca ctccccggcc gatcgcgcgg
     2881 gcgcgcggcc ggggatgccg gcgaggcgcg ccaggccgag cgcgaccggt tcgcccgcgg
     2941 tcaccgagcg ccaggggccg gcggccttcg ccggcagctc gcccgcgccc cacagccaga
     3001 cactgttcac gggaagctcc cgcgcctcgt tcaccggatg ctcgtgcagc gccatctgga
     3061 tctcgttcag cagcgcgggc cacttgccgc cgggcaattg cgggtcgatg tcgcgccccg
     3121 ccatctcggc ggcggagagc gtggcgagcg cggcgggcgc ggtgcgcagc acccaggcat
     3181 ccggctgcgc gacgcggaat tcgtgcaggc cggcgaaatg ccggttgagc gtgtccacca
     3241 gcgcctcggc ctcggcgcgc gccaggccgg tcaccgggac cagcaccagg cggtcgcgca
     3301 tcacgcgcaa gtgcaccggg tcggcgcgca gccagaatcc gctgtcgaaa gctgtcagcg
     3361 cgccggccgg cagcggatcg acgccgaagg cttcgcccag ccaggcatgc agcccctgcg
     3421 tctcgcaggc ggtggcgcgc gcgcgcgcca gcagcagctc cagcgcgggc agccgcggct
     3481 ccggcggcgc cgcgagcagc cccgggacga tcagctcggc gtgcagggca gctccctcac
     3541 gcgcgcgcgt ttcgtgccga ggtagcgggc gagcagcttg tactggtcgg ggtcgaaggc
     3601 caggcgccgc gattgctcga gcagcgccgg cacctcgtcc tcgctgcgcg cggtgccgag
     3661 gtagcaccag cggtcgatga cgtgcagctc ggccgcgtcg cgcgcgcggt cttcctccac
     3721 cacgcccacc ggcccgcgcc agggccaggc gagcgcaggc agcgcggcga gcgcggcggc
     3781 gaggcgcgca tggtgcgcct gcacgccttc cttgccggcg cacagtccag cgcagcgcgc
     3841 gatctggcgg cggaagcacg cgcccttgcg cgcgggctcg aagcccagcg attgcaggca
     3901 cagcccatgc tcgtcggcga ggccgcgcag cgcctcgagc gccgcgcgac gcgtgcggaa
     3961 caggccgcgc agctgcgcga ggtgctcgag cccgaagtcc tccgcgaggc gcagcttctt
     4021 gccgtcgaaa gaaaacgcgc agagctgcgc ggcgcggcgc agctgccggt tgaacgcggg
     4081 cgcgagctcc ttcaccagcc gcgcttccag cagcaaggcg cccagctccc cggcgcagcg
     4141 ccgccactcg atgcggcgca cctcgcgcgc catctgcatc tcgcgcgggg agcgcaggtc
     4201 atcgccgaag tgctgcagca cgcgagcgcg catgtcgacg ctcttgccga tgtacagggg
     4261 cgcggcgccc tcgccgtaga agaggtacac gccgggtgac tcgggcaccg cgtccacctt
     4321 ggcgcggtcc aggtgcgcgg gcagcgtcgg ctgcttcgcc acctggcgcg cggcgaccgc
     4381 caccacctcc tcgccgtgct cggcggcggc gaggcgcagg aactgccata ccgcgtcggc
     4441 gtcgcccatg gcgcggtggc gcgcccggca ctcgatgccg tggcgctgca tcacgctgtc
     4501 caggttgtgg cgcgcctgcg cggggtacag gcgccgcgac agcttcaccg tgcagagggt
     4561 cctcgcgcgg tacggcagcc cggcgcgctc gaactcctgc cggaggaagc cgtagtcgaa
     4621 gcgcgcgttg tgcgccacca gcacgcggcc cgcgagccgc tcgtgcagct gcgccgccag
     4681 cgcgccgaag ctcggcgcct ccgccaccat gtcgttggtg atgccggtga gcgcctggat
     4741 cgccggcggg atcgaggtgc cggggttgac cagcgtgctc cactcgccgg cgacttcgcc
     4801 gccgtccacc tcgatcaccg cgatctcggt gacgcggtcg tgggccgggt gcgcgccggt
     4861 ggtctccagg tcgacgatcg ccagcggcgc gtcgagcagc tgcatcactg cactttgacg
     4921 ttcgccgcct tgatcacctg cgcccacttc gcggcgtcgg acttgatgcg ctcgacgaac
     4981 tgctcgggcg tgctgcccac ggcttccgcg ccggcggcgg cgaagcgctc cagcaggtcg
     5041 ggcgccttga gcgcggcgag cacctcgccc tgcacccggt cgacgatcgc cgcgggcgtg
     5101 ccggccggcg cgaacatgcc gaaccaggtg tagcggtcga aggccggcat gccggcctcg
     5161 gcgagggtgg gcacctcggg catcagcgcc gagcgcttct gcgcattcac cgccagcggc
     5221 cgcacgcgcc ccgccttgat gtgcggctgc acgctcgcca ggctgtcgaa cagcatggcg
     5281 atgctgccgt tggccacgtc cgggatggag agcgccgtgc ccttgtaggg aacgtgcacc
     5341 aggtcgacgc cggcgagcat cttgaacagc tcggcgttca gatgcacgat ggtgccgacg
     5401 ccgctggagg cgaaattgag ctggccgggt ttcgacttgg cgagcgcgat gagctccgcc
     5461 acgcttccca ccggcagctt ggggctgacc accaggacgt tcggcacgtt ggcgacgtgc
     5521 gtgatcggcg cgaaatcctt cagcgggtcg tagggcagct tctgcagcac cgggccgatc
     5581 gagtgcgtgc tggaagtggc gatcagcagg gtgtagccgt cgggcgagct cttcgccacc
     5641 aggtccgagc cgatcgagcc gccggcgccg gggcggttct ccaccaccac cgactggccc
     5701 agctgctcgc cgagcttctg cgccacgatg cgcccgacga tgtcggtggc gcccgccggc
     5761 gggaaaggca cgatcagctt gacggggcgg ttcgggtact gcgcctgcgc ggcgcaggcg
     5821 aagacggcaa ccaggagcgc gacggcgatt ctcatgatca tccctgtata aatgtccagt
     5881 gcggcaactc taccgcaaaa gctgcgcgaa ctgccatgcg gcgaagccga ggatgctgaa
     5941 gccggcaacg acgttgaccg cgcgcagcag gggcgggccg aggcgcgcgc gcagcttgcc
     6001 ggcgaacgcg gcgagcagca cccaccaggc tgccgagccg aggaacaccc ccagggtcac
     6061 cagcgccgcg gcggcgtagt cggcgctcgc cgcgagcccc aggccggcga agatcgcggc
     6121 gaaggcgagg atggtgggcg ggttggcgag cgtgaggacg agcgtggagg cgaaggcctg
     6181 ggcgaggctg gcgggccttg ccgtctcgcc gtcggccacg ggatgcgaca ggagcgcctt
     6241 cacgcccaga taggcgagga aaatcccgcc ggcgaggccg atccagaagg cctgcgcgag
     6301 caggaagcct gagattgcgg tgaggccgaa ggcggccacc gcgccgtaca ggccgtcggc
     6361 cgccgcgatg cccatgcccg agacgaagcc ggtgagcgcg ccgtaggcga gggtgcggcg
     6421 caggcacagc acccacatcg ggcccaccgt ggcggccagc acgaagccga acgccaggcc
     6481 ctttagaaac agcaccggaa cagcactttc atccgagcgc gacgtgcccg ggctgcgcct
     6541 tcacccgggc gagccaggcg gcgaccgccg gatagcgcgc gaggtcgaag ccgccgtcgg
     6601 cggcgcaatg cgtgtaggcg tagagcgcga tgtcggccac cgtatagcgg ccgccggcga
     6661 accagggcgc ctgcgccagg tgctgctcca tcaccgcgag cgcctggtgg ccgcgctcct
     6721 gcagccgcgg cagctcggcg cgccgcggcg tgccggcggg gaggtagcgc aggatgaagc
     6781 gcgccaccgc cacatagggc tcgtggctgt actgctcgaa gaacatccac tgcaggacgc
     6841 gcgcgcggcc ggcgcggtcg ccgggcaaca gcggggtgcc gtcggcgagg tagaacagga
     6901 tcgcgttcga ctcgggcagg tggctgccgt cctcgagctg cagggtgggc accttgccgt
     6961 tggggttgcg cgcgaggaat tccggcgtgc gcgtgccgcc cttggtggaa tcgacctcca
     7021 cccagcggta gggcagcgcg agagtttcga gggcgagctt caccttgtag cagttgcccg
     7081 agtccgccat gccgtacacc gtgaccatgc cttcccccta gtgccagtac ctgcgcagcc
     7141 atttcgccgc cggcgcgagg tccacctggt tggcgaaagc gaccatctcg gcgatgcggt
     7201 ccacgctgtc gaggtcgaag gcctcgcccg gcgcgaagga cggcgccacg cgatggtaat
     7261 ggcgcccgag catctgccgg cactggaagt cggcgatgcc gatggtgccg tcgaacatca
     7321 cgccgagcag cgcctgcgcc cactgcgcgt agccccagtc gtgccggtcg cccttcacgt
     7381 agatgagcga gctgccggtg cccagcgaca gcagcaccac ctcctccagc ggcgcggtgc
     7441 gcccgatgcg cgcgtcctgg ctctgcgcca gcgcgcacat cgaggggttg ttggcgaaca
     7501 cgccgccgtc cacgtagccg tccacggtgg gaaagtaggt cggcgcggcg gtggtgtaca
     7561 ggcccgcctt ccacaccggc atggcgccgt cgctgtcgga gcctgggaaa ttgtggaaca
     7621 gcttcggctt ccagcgccgc cgcgcgggat tggggtcctc gttgtccagg tcgaaggcgg
     7681 tgaccagcac gcgctttttc aggtcgcgca ggcgcgccgc gccgaacagg cgccgcagct
     7741 cgcgctgcag gccgtcggaa tggtactggg cgccgaggat cgtgcccgcg tccttcacgt
     7801 tgtcgagcca ggagtcgtcg aagatcgtcg cggccttgtc gtggtacagc gcgaggatct
     7861 cctccagcgc gaggccccgg gcgagggcga gcgcgatcag cgcgccggtc gaggtgccgg
     7921 cgaggagctg ggcgcgctcc agccagcccg cgagcccctt ttcccgggcc aggcgcatga
     7981 ggaggatggt cgaaatcagc ccgcggatgc cgccgccgtc gaggctgagg atgcgatagg
     8041 ccattcaatg cattgtagta ttctcggcct cccgtgcgtt acgagctcct cgtcggcctg
     8101 cgctacaccc gcgccaagcg ccgcaaccac ttcatcagct tcatctcgct catttccatg
     8161 gcgggcatcg cgctcggcgt ggcggcgctg atcgtggtgc tgtcggtgat gaacggcttc
     8221 cagcaggagc tgcgccagcg catcctggcg gtggtgtcgc acgtgcagct ctccggcccg
     8281 ggcaaccggc tcgacgactg gcgcgcggtc gccaaggggg cgctcgagca cccgcgcgtg
     8341 cgcgaggcgg cgccctacat caacgcgcag ggcatgctca cgcacgggca gggcgtgcgc
     8401 ggcgccatcg tgcgcggcgt gctgccggcg gaggaggagc gcgtcgccga catcggccgc
     8461 cacatgcgct cgggcagcct cgccgagctg aagcccggcg gcttcggcat cgtgctcggc
     8521 ggggagctgg cgcgcgcgct cggcgcgctg cccggcgaca aggtggcgct catcgcgccg
     8581 caggggcagg tgacccctgc cggcgtgatc ccgcggctga agcagttcac cgtcgtcggc
     8641 actttcgaca tcggcatgtt cgagtacgac tcgagcctcg cgctgatgca cctcgcggac
     8701 gcgcagacgc tgtaccgcct cggcggcgcg gtgtccggcg tgcgcctcaa gctcgaagac
     8761 ctgttcgccg cgcgcaccgt gtcggcgcag ctgatggcga gcctgggggc cggcgtgttc
     8821 gcctccgact ggacgcgcag ccacgccaat ttcttccgcg cggtggagat cgagaagcgc
     8881 gtgatgttca tcatcctcac gctgatcgtg gcggtggcgg cgttcaacat cgtctccacc
     8941 ctggtgatgc tggtcaccga caagcaggcc gacatcgcca tcctgcggac gctgggcgcg
     9001 gcgccggcga gcatcctgca gatcttcgtg gtgcagggcg cgctgatcgg cgtcatcggc
     9061 gcgctgatcg gcgttgccgg cggcgtgctg ctcgccctca acgtgggcac ggtggtgccg
     9121 gcgatcgaga gcctgctcgg cttcaagttc ctcgccaagg acgtgtacta catctccgac
     9181 ctgccctcgg acgtgcagct gcgcgacgtg gtcaccatcg gcctggtgtc gctcgccatg
     9241 tccttcgtgg cgacgctgta cccgagctgg cgcgcctcgc gcgtcaatcc ggcggaggcg
     9301 ctgcgctatg agtgaggcgg tgctcgagtg caggggcctc gccaagacct accagggccc
     9361 gcagccggtc gaggtgctgc gcggcgtgga tttcgcggtg cgcgcgcgcg agcgcgtggc
     9421 gatcatgggc aagtcgggct cgggcaagtc gacgctcctg cacctgctcg gcgggctgga
     9481 tgcgcccacc gcgggcgcgg tcagcgtgca gggccgcgcg ttcagcgcga tgggcgaggc
     9541 cgagcgcggg caggtgagga acgccacgct cggcttcgtc taccagttcc accacctgct
     9601 gccggagttc accgcgatcg agaacgtggc catgccgctc ctcatccggc gcatggaccg
     9661 cgcccaggcg ctcgacaagg cgcgcgccac gcttgccgaa atcggcctcg cgcagcgcct
     9721 gcagcacctg ccgggcgagc tctcgggcgg cgagcgccag cgcgcggcct tcgcgcgcgc
     9781 gctggtcacc gagccggcct gcgtgctggc ggacgagccc accggcaacc tcgacacgca
     9841 tacctcggac gaagtgttcg aggcgatggt gaagctctcg gcgctgcgcg gcacggcctt
     9901 cgtgctggtc acgcacgacg ccgcgctcgc ggcgcgcgcc gagcgcgtgc tcgagctgcg
     9961 cgacggcttg ctgcagcaaa agcgctaggc ggccgcatgg cgctcctgca gctgctgctg
    10021 ttcctcctgc tgtgcgcgct ggcgctcggc tggctgtcgc ggcgcgtcaa cctgccctac
    10081 ccgatcgcgc tggtgatcgg cggcagcctg ctcggcttcg tgccgggcct gccgcaggtg
    10141 gagttcgacc cggccttcct cctcgtgctg gtgctgccgc cgatcctcta ccaggcggcg
    10201 ctgctcacct cctggcgcga cttcaaggcc aacctgaagc cgatcggctt cctcgccatc
    10261 ggcctggtga tcgtcaccac gctcgcggtg ggcgcggtgc tgaagctcct ggtgcccggc
    10321 atcccctggg cggtggcctt cgccttcggc gccatcgtct cgccgcccga cgcggtggcg
    10381 gcgaccgcgg tgctctcgcg ctacaacatt ccgcaccgcg tggtggtggt gctggagggc
    10441 gagagcctgg tgaacgacgc ttccggcctg gtgctgtaca agttcgcggt ggcggcggtg
    10501 ctcaccggcg cgttctcgct ggcggaggcg agcgtgcagt tcgtgctggt gtcggcgggc
    10561 ggcatcgccg tggggatgct catcgcctgg gtgttcgtgc agctgcaccg gcacatcggc
    10621 gacccgttca tcgaggtgct ggtgtcgctg atgatcccct acggcgccta catcgccgcc
    10681 gagtcggcgc acctctcggg cgtgctggcg gtggtggcgg cggggctgtt ccgcgggcgc
    10741 cacgcgccga agatcgtctc ggcggagatg cgcatcctcg cgcgctcggt gtggaacatc
    10801 ctggtgttca tgctcaacag cctgatcttc atcctcatcg gcgtcgagct gcgcgtgatc
    10861 gtcggccggc tggacggcta cagcgcgggc gagctggcgt ggactgcgat cgcggtgagc
    10921 gcggcggcga tcctggtgcg cttcctgtgg gtgtacccgg cggcccatgc cgcgcacgcc
    10981 gcgcgcgccg cgccgctgcc gccgcgcggg cagctgttcc tcgtcagctg gtgcggcatg
    11041 cgcggcatcg tgtcgctcgc cgcggcgctc gccctgccgc tggagacctt cgcgggcgag
    11101 cctttcccct accgcgacct cgtcgtgttc ctcaccttcg tcgtgatcct ggtgaccctg
    11161 gtggggcagg gactgacgct ggcgcccatc atccggcgcc tgggcctcgg ctcggaagcg
    11221 ggactgcgcg ccgagcagct gcgcgcgcgc tccgacatgg ggcgcgcggc gctgctggcg
    11281 atcgatgcgc acgccgagga gcacggcgtg gcggcgccgc tcgccgcgcg cgtgcgcgcc
    11341 gaattcgccg agaagatcgc gcaggagacg cccgccgggc cggtagagcc ggcggacgcg
    11401 gggccggcgc gcgcgctgcg cctggccgcg ctgcgcgccg agcgcgcgga actcatccgc
    11461 gtctggcagc tcaaccacat cagcgacgac gtgctgcacc acctcgagga agaactggac
    11521 taccaggaat cgcggttatg aggccggcgg ccagggcttc ggcgtagaag cgctcgtcgg
    11581 cgagggcgcg ctgcaggacg tagccgccgg cggagcggcg cacggcatgg cgcagggcaa
    11641 gggcggcggc gaacagcgcg ccgccataga agtagtggcc gacgagcgcc agcgccaccg
    11701 ccgccccgag cgccggcagc agcacgaacg gcagcatcag ccgcgcggac agcagttgcg
    11761 ccgcgccctt gggatccacc tgcacgcggg cgccgcgcca ggcgtcgacg aactccgcgt
    11821 gcgtcattcc tgctgccgct tgcgctgccg cgcgcgccag gcgagcacca cgtaggcgcg
    11881 ccagccgacc tgcaccgcga cgtagccgag cgcggcgagc gtcagtccca gcgccaccag
    11941 gccgacgagc agcggcttgc ccagcgccag tccccagtcg agcatgcgcg ccatcgagtc
    12001 gaggaagtgg ccccagtcca tgtcgaaggg ctcgatcttc gcttcgccgt gcgagccgcc
    12061 gagcaggagg ccgccgtagc cgtaggcgag gaggtacagc ggcacgatgg tgaagggatt
    12121 ggtgtagagc gtcatcagca gcgccaccgg caggttgcgc cgcagcggca ccgcgaggag
    12181 gagcgccatc agcatctgca gcggacccgg caccagcccg gcgaacaggc cgatcgccac
    12241 cgcgcccgcc accgagtcgc ggttcaggtg ccacaggttg gggtggcgca gcagcgagcc
    12301 gaacatcccg accaggcggt tctggcgcac gctggtggcg ctcggcagga acttgcggaa
    12361 caggtcgcgc ggcagcatcg ggtcatccgg cgcgagggcc ggcgcgttga ttttagatga
    12421 ccggggccgc gctagcgttc accgccgggg tgctgctcct gcagcagcag gcggcgctgc
    12481 cggcgccggc gtggctgctc ctgctgccct gctgcgcggc gctcgccgcc cggcggcgcg
    12541 cgctcgtcct gcccgctgcc cttgccatcg gcttcctgtg ggcggcgggg tgcgcgcacc
    12601 tgcggctcgc ggatcgcctg gcgccggaac tggagggccg ggacctcgac gtcgcgggcg
    12661 tcgtcgccgg cctgccggcg gcgagcgagc gcggactgcg cttcgcgttc gacgtcgagt
    12721 cggccgcggc gcgcctgccg cagcgcatcc agctcgcctg gtaccgcaac gcgtggcgcg
    12781 aggacgcggc cgcggccgtg cccgagacgc tgcgccccgg cgaacgctgg cagttcacgg
    12841 tgcggctgcg ccagccgcac gggcacgcca acccgcacgg cttcgactac gaggcctggc
    12901 tcaccgagcg cggcatcggc gccacgggct acgtgcgcca gggcgcggcg gcgcggcgcc
    12961 tcggcgagcg cgaaagcctg ctcgatcgca tcgaacgcac gcgcgcagcg gtgcgcgacc
    13021 gcttcaagtc ggtgctcggc gagacgccct ctgccggcat cctcgccgcg ctcgcggtcg
    13081 gcgaccagcg cgccatctcg agcgaggagt ggcggctgtt cggccgcacc ggggtcacgc
    13141 acctgatgag catctcgggg ctgcacgtga cgctggtctc gggcctcgcc gcctggctcg
    13201 tgtcggcgct ctggcgcagg gtgccgcgcc tcgcgcttgc cctgcccgcg cgcaaggcgg
    13261 gcgccgcggc ggccatcctg gcggcgctcg cctacacgct gctcgccggt ttcgcggtgc
    13321 ccgcgcaacg caccttctat atggtgaccg tggtggccct cgccctgtgg tccggccgca
    13381 tcgcctcgcc cgtgcgcacg ctggcgctgg cgctcgccgt ggtggcggcg ctcgacccct
    13441 gggcggtgct gcagcccggc ttctggctgt ccttcggcgc ggtggcgctg atcttccacg
    13501 tcacggcggg atggacgcaa agcgggccgc gcgcgctgca gtggctgcgc gtgcaatggg
    13561 cgatcaccgt cggcctcgcc ccggcggcgc tgttcctctt cgcgcagctg tcggtggccg
    13621 gcccgctcgc caacgcgctc gccatcccgc tggtgtccgc cgtgatcacg ccgctcgcgc
    13681 tcgccgcggc gctgctgccc gccgatgcgc tgctgctcgg ggcgcaaggg ctcaccgagt
    13741 ggctgctcgt cgccctcgag tggtgcgcgc agctgcccgc ggcggtatgg cagcagcacg
    13801 cgccgccgct ctgggccacg ctgctcgcgc tcgccggcgc ggcgtggctg ctgctgccgc
    13861 gcggctttcc ctggcgcgcc agcggcctcg cgctgatgct gccggcggcg ctgctgccgg
    13921 cgccggcgcc gccgcccggc gaggcctggg tcacggtgct cgacgtgggg cagggactgg
    13981 cggtgctggt gcgaaccgcg aaccacgcgc tgctgtacga cgccggcccg gcctacggcg
    14041 cggaagccga cagcggcgag cgcctggtgg tgccggcgct gcgcgcgctc ggcgtggcgc
    14101 gcctcgacgc gctggtgctg acgcacaacg acgccgacca cacgggcggc gcgcagtcgg
    14161 tgctcgacaa cctggaagtc ggcgcgctgc gcacgtcgct ccccgggcgc cacccgctgc
    14221 tcgcgatggc ggcggcgccg gcgcgctgcg cgcgcggcca gtcctgggac tgggacgggg
    14281 tgcagttcga gttcctgcat ccgccgccgg gcgcgcccgc gcggcgccgc aacgacgaca
    14341 gctgcgtgct gcgcgtggcg gccgccggcg cggcgatgct gctggccggg gacatcgagc
    14401 gcggcgccga aggccggctg ctggacgcgg ccgcgctgcg cgccgatgtc ttgctggtgc
    14461 cgcaccacgg cagccgcagc tcctcgagcg aagccttcct cgacgcggtg cgcccgcgcc
    14521 tggcggtcgc cgccgcgggc taccgcagcc gcttcggcca cccgcatgcc gaagtcctcg
    14581 agcgctacgc cgcccgcggc atcggcgtgc tgcgcaccga cctcgacggc gcggtcagcg
    14641 tgcggctcgc gcccggcggc gcgagcgcag cgggcgagcg cgcgcgccgc gcgcgctact
    14701 ggcacgttcc cgcccccgca gcgtaaactg gccgcgcggg attcccccgc ccacgctcaa
    14761 ggaggagctt tcatggccaa aggcagcaat gcggtctacc gcgtcaccga tgtcatcggc
    14821 accagcccga attcctggga ggaggcggcg cgcaacgcgg tgaagaccgc ggccaagtcg
    14881 ctgcgcgacc tgcgcatcgc cgaagtcacc aagctcgacg tgaaggtgga ggacggcaag
    14941 gtcacgcagt tccgcacgcg cctgtcgctg tcgttcaagt acagcgcctg agccgcggca
    15001 accgcgttcc ggacggcgaa tcacgacgct agggcgcggg agatcgacgc atgctggtgc
    15061 tgctgcgcct gattcgcaag atcgtcggct gggtggattt cctggcggtg tgcctggtcg
    15121 tctacctgct cgcctggctg ccgtggcccg gcaagcaccc ggtggcgcgg ctgtttcccg
    15181 tctggtgccg cgccttcgcg cgcgcgctcg acgtggacat ccgcgtgcac cagaagcacc
    15241 gccgccagca cctgcccgag cgctacatcc tgatcgccaa ccacccgtcg gccttcgagg
    15301 acatcggcat cccggccgtg ttcgacgtgg tgtcggtggc gaagctgcag gtgaaagact
    15361 ggttcatcgc cgggcggatc gccagggcgg ccggcaccct gttcgtcgac cgcgataacc
    15421 cggaatcccg caagcaggtc atcgacgaga tggtgcaggc ggtgagcggg gggcggaaca
    15481 tcgccctcta tccggaaggc ggctgcaagg ggcgccgcct gcacagcgag ttcaagaccg
    15541 gggcgttcga ggtttccttc cgcaccggga ttccgatcct gccgctgttc ctgcactacg
    15601 aggcgcagga cgatttcgag tggcagccgc cctacacgct gccggacaag atccggcaca
    15661 tcatgaccgc ggcgaacaac cgcgcgaact actacgtcta cgaaccgctc gacccgaagg
    15721 actacgccga ccgctacgcg atgaaggcgg cggcgtacgc gctctacacg cgctggaacg
    15781 cccagtacct cgaatagact ggaggccgat ggccaccgcg atcgttcgcg tcaccggcgg
    15841 cgtgggccgc ttcgaggagt tccgcgagcg catccgctgg ctgctggtgc gcgatcccga
    15901 cgtcgatccc cacacggagc atcacgcgcc cgaccggctc gagtaccgct tcgcgctcgt
    15961 caagggcatc ccctttccct ccttcgtcgc ggcgagcggc gaataccccg agctgcgcgt
    16021 cgaggttcag tgggacaaag gcggggacca ggccggggcg aagggcggcg tggcgatcga
    16081 gcagggccgg atcgtcgagc agtgggcggg ggagcgcaag cccgggtgag cgtcgagact
    16141 cgcctgcgct acgtgcagtg cgcctcgccc agggggctgc accgcattgc ctaccgcgag
    16201 agcggcgacc cgcaggcacc ggcgctggtg tgcgtgcacg gccttacgcg ttgcgcgcgc
    16261 gacttcgacg cactggcagc cgcgatgagc gaccgctacc gcgtcatctg cccggacgtc
    16321 gccggccgcg gcgactcgga ctggctcgcc gatccgatgc tctaccagct gccgcagtac
    16381 gtcgccgacc tggtcacgct ggtggcgcgc ctcgacgtcg agcgggtgca ctgggtcggc
    16441 acctcgatgg gcggcctcat cggcatggcg ctcgctgcgc agaagaactc gccggtgtcg
    16501 aagctggtga tgaacgacgc cgggccggtc atcgccaagg cgtcgctcca gcggatcggc
    16561 agctacgtcg gccttgcgcc ggcgttcgcg accatcgagg ccgccgagca gtacgtgcgc
    16621 accgtgcacg cgccgttcgg cccgcacagc gatacccagt ggcgcttcct caccgaggcg
    16681 gtggtgcgca ggaacgccga cggcagctac cggctgcact acgatccgaa gatcggcgag
    16741 cctttccgcc agaacatgcc ggcgggcgac ctggagagtt ggccgctgtg ggacgcgata
    16801 cgctgcccgg cgctggtgct gcgcggcgcg cagtcggatc tgctgtcgaa ggccaccgct
    16861 caagaaatga ctcagcgcgg gccgaaggcg aaggtggtgg aattcgccgg cgtcggccat
    16921 gcaccgacgc tgatgcacgc cgaccagatc gcggcggtgc gggagttcct gctgtcctga
    16981 gtcagccgcg gcggttcgcc gcgtcccagt gccgctcgag attgtcgatc agcgccgggc
    17041 tgaaatggca ctgcgcctgc tcgcgcgcgt acaccgccat gtagcgccgg aaggtgtcga
    17101 gcggctcttc accggcgcgg aacgcgcgct tgttgcccgc ggcgctcacc acgccggaat
    17161 tggtgaggtg ttcgatgctg cgcgcgatcg cctggtccat ccccgccgcc ggcacgatct
    17221 cgtcgcagat catgcgccct tcggcagacg cgcagtccaa ccgccgctcg gcgaggatcg
    17281 cctggcgcgc gatgcgcgcg ccgacgaagc gcggcagcct gaggttggcc gcgcccggga
    17341 tgatgccttc cttccgcgcc ggcagcgtca tgtaggcatc ctccgccgcg agcacgtagt
    17401 ccatggtgag caggatctgg cagtgcccgc cgatggcgaa gcgctccacc gccgccaccc
    17461 agggcttctc gcggcaatcc acctcggggt tcacgcccgg caatgcgagg ccgcggtaca
    17521 tcttgttgac gaagccgagg tcccggatca ggtaccagag gaaccggatc tttccctggt
    17581 acaggtgcgt gaggttgatc ccggcgccga acacgcgctg gcccgcgtgc ttggtgtgcg
    17641 tgaccacgtc gccgcgcagc acgcagagtt cggacttcct gtccatcaac gccacgtcca
    17701 ccgcgatctc catgccctcg agagtggtct cgtcctcggc attgaggtag cgcggattgc
    17761 gcatggtgag caccacggcc ttgccctggc gcgcgagctg cgcgccggcg agagacagct
    17821 cgcccttcgc ctcgaattcc gcgagcgcct tggccgaatc cgcgcgcggc aggagcatgg
    17881 cgtggcacag gtgcgcgccg gcggccgggt cagcgagcac cgcggcgagg aaggcgccct
    17941 gggccttctc caggccgcgc ttgtcgcgct gcgccagggc ggcctccgcc tcgaggtcct
    18001 tggcccccgg gacgaggccc gggtagcgct cggcggcctg gcggcacagc tcgtccagcg
    18061 gcaggaaacg cgtgcgcccg gcggtgaggg cgtcgtaggc ggcgagggga tccacgcgcc
    18121 cagtctaccc aaatcccgcc cgccgctgga cattcgctgc ccaggcaggt agtttgctgc
    18181 ttcggcagct aatcaggggg cggcatgggc ttgtaccgcg cggaaaccta ccgggtcgag
    18241 gacagcgtcg gctacctggt gacgcgcgtg cgcgcctcga tcttcgccgc ggtggacgcc
    18301 gagctcgccg actggggcat ctcggccgcg cagggctcga tcctggtcta catcggccac
    18361 ggccgcggcg accgcgccgc ggacatcgcg cgcgactacg actacgacac cggctcgatg
    18421 acgcgcatgg tggcgcgcct gtcggccaag cgcctgctgc gccgcgtgcc cgacgagaac
    18481 gaccggcggg tggtgcggct cgatctcacc gccgcgggcc ggaagctcac cgagcagatg
    18541 gcggcggtgg cggccaaggt gctcaaccag cacctgcgcg gcttttcccg cgccgagctc
    18601 gagcagctga aggcgctgct gcaccgcatg ctggacagcg cccgtggcta gccggcgccg
    18661 cgcgctgacg ctgctcatcc tgctgttcgc cggcgcgggc gtggcctggg cggcgtggtg
    18721 gcaggtcgtc ggccggcatt acgagagcac cgacaacgcc tacgtcgccg gcaacctggt
    18781 ggaggtgacc tcgcaggtgg cgggcaccgt gatcgccatc ggcgccgacg acaccgagcg
    18841 cgtcgaggcg gggcaggtgc tggtgcgcct ggacccggcg gacgccagga tggcgctgga
    18901 gcaggccgag gcggagctgg cgcgcgcggt gcgcgacgcg cggacgctgt acgccaccaa
    18961 cctgtcgctc gaggcgaacg tgcgcgtgcg ccaggccgag ctgcagcgcg ccgaggagtt
    19021 cgtcgcccgg cgcaccgcgc tcgcggggca gggcgtggtg tcgcgcgagg actacgacaa
    19081 cgcgcgcagc ggcgcggcgg cggcgcgcgc cgcgctcgcc tcggcgcgcg agcagctggc
    19141 caccaaccag gtgcgcaccg agcgcgtgcg cgtcgaggac cagccgggcg tgctgggcgc
    19201 ggcggcgcgc gtgcgcgagg cctggctcgc gctccggcgc accgaggtcc cggcgccggt
    19261 gagcggcatg ctggcgcggc gctcggtgca ggtcggccag cgcgtcgcgc ccggcgcgcc
    19321 gctgatggtg gtggtgccgc tggagcaggt ctgggtggac gccaatttca aggaagtgca
    19381 gctgcgcgac atgcgcatcg gccagccggt gcgcctgagc gccgacctgt acggcggcga
    19441 agtgcagttc cgcggcaagg tggcgggcct ggcggccggc accggcggcg ccttcgcgct
    19501 gctgccgccg cagaacgcca ccggcaactg gatcaaggtg gtgcagcgcc tgccggtgcg
    19561 catcgcgctc gacgcggagg agctgcgccg ccaccccttg cgcatcggcc tgtcgatgcg
    19621 cgccgaggtg gagatccgcg acaagagcgg caggcagctg gcgagcgcgc cgcgcagcgg
    19681 cacggcctac gccaccgagg ccttcgccgc ctcgcccgcc gaagccgagg cgcgcgtgcg
    19741 cgccatcatc gccgcgcacc ggtgagcgcc gcggcgcccg ccgtcgctgc ccccgccccg
    19801 gcaccgctcg ccggcgccga gcgcatgctc ggcgcggtga tgctcgcggc ggccaccttc
    19861 atggtggtgc tggacatcac cgtggccaac gtctcggtgg ccaccatcgc cggcgacctc
    19921 ggcgtgagcg ccacgcaggg cacctggatc gtcacctcct acgccgcggc gcaggccatc
    19981 gccatgccgc tcaccggctg gctgacgcgg cgcttcggcc aggtgcggct gatcgtggcc
    20041 tcggtgctgc tgttcaccgc ggcgtcctgg ctctgcggcc tggccgagag cctggagacg
    20101 ctggtggcgt tccgcgtcct gcagggcctg gtcgccggcc cgatggtgcc ggtgtcgcaa
    20161 tcgctgctgc tcgccacttt cgggcccggc ggcgccggca tggcgctcgc cgtctggtcg
    20221 atgaccgcca ccatcgcgcc gatcaccggc ccgatcatcg gcggctacat caccgagcag
    20281 atcgcctggc cgtggatctt ctacatcaac gtgccggtcg gcctgctggc cggcggcatg
    20341 atctggctgc tgtaccgcga gcgcgagacg ccgcgccagc gcgtgccggt ggacgtggtg
    20401 ggcctggtgc tgctcacggt ctgggtggcg gcgctgcagg tgatgctcga caagggcaag
    20461 gagctcgact ggttcgcctc cggggagatc gtggcgctgg cctgcgcggc gggcctgggc
    20521 ttcgccctgt tcctcgtctg ggaactgctc gacaacccgc acccggtggt cgacctgcag
    20581 ctgctgcgcg accgagattt ctggaccgcc accgcgacgc tctcggtggg ctacgcggtg
    20641 ttcttcggcg ccatcgtggt gctgccgctg tggctgcagc agaccatggg ctacacgcag
    20701 atctgggccg ggctgatgat gatgcccttc ggcgtgctgg cgctggtcct ggcgccggtc
    20761 atcgcgcgca ccctgcacct ggtggacgcg cgcctggtgg cgagcctggg gttcctggtg
    20821 ttcgcgctgg tgtcgttcat gcgcgcaggg ttcgacatga cggccgactg gatgaccatc
    20881 gcgctgccga tgttcatcca gggcgcgggc aacgtgatgt tcttcctgcc gctcaccgcg
    20941 ctcggcctgc gcggcatgtc ggcggacaag atgcccggcg ccaccgggct gcagaatttc
    21001 ctgcgctaca tgggcggcgc ggtcggcgcc tcgctcgccg tcaccctgtg ggacgaccgc
    21061 gccaagctgc accgcgcgca cctcgccgag cacctgacgc gcggcgaccc ggcgaccgag
    21121 ggcgcgctgc gcgccctcga ggcgcaggga ctgggcgggg cgcaggcgct cgcgcacctc
    21181 gatcgcgcgc tcgaggtgga ggcgcgcatg ctgagcaccg tggacctgtt ctggatctcg
    21241 ggcgtgctgt tcctcgcgct ggcggcgctg gtgtggctgg cgaacccgcc gcgcgccgct
    21301 gctaagatgc acgctgcaac cgggagccac gagtgaagga cttcaccccc accacgcgca
    21361 ccaggatcaa gcgcctgccc aagcgggcgc gctacgaccg cgacagcgtc tacgccatcc
    21421 tcgacgcggg cttcgtctgc cacatcggct acgtcatcga cgggcacccg ttcgtcacgc
    21481 cgaccgccta ctggcgcgag ggcgacgcgg tgtactggca cggctcgtcc aagagccgca
    21541 tgctgatggc gctgcagaag agcccgaagg tctgcctcac cgtgatgcac ctggacggcc
    21601 tggtggtggc gcgctcgggc ttccacaagt ccatcaacta ccgctcggcg atgatcttcg
    21661 gcaagcccta ccaggtgacc gagccgcagg agaagctggc caagatggaa aagttcgtcg
    21721 agcgcctcta tcccgggcgc tggccgggcc tgcgcgcggt gcacaagcag gagctgaagg
    21781 ccaccacggt gctcggcatg cacatcgacg aggcggtggc caaggtgcgc agcggcgggc
    21841 cgatcgacga cgacgaggac tatcggctgc cgatctgggc gggcgtgttt ccggtgcggc
    21901 aggtgctggg cgagccggtg gacgacgggc ggctggcgcc gggcgccccc ggcgcgccaa
    21961 gggtcaccct ctagaggatt cccgccgcga tccccgcggc cattgcggcg ccgagctcct
    22021 cgcaggcggc gagctgttcc ggtttcaatt ccttcaccac caccaccggc tcggcgatct
    22081 tcttcagcct caggccggtg acgatgcgct cgaccgaggt caccgcgccg ctgccgtcct
    22141 gccccgcgcc gacgaagagc gcccagggcc tgccctcgac cttgccctcg caggcgtaga
    22201 acacgcgctc gaggaagtcc ttcatcatcc ccgccatgta gccgaagttc tccggcgtgc
    22261 cgaggatcag gccgtcggcg ccgagcaggt cctccggccc ggcctcggcg cagcgcttca
    22321 ccaccacgcg cacgccttcc tccgagcgcg cgccgcgctc cacggcctcg gccatccgcg
    22381 cggtcttcgc gccgccggtg tggtagacga tgaggagggt cttcatgttg ccaggcgcat
    22441 cgccgggtcc tgccggtagt accgcttgag ctgctcgtag gcgtcgggat agcgccgccg
    22501 cgtctcattc ggcgacgcga agaacgcctc gctcatcacc gcgaagaact cccccggcga
    22561 ctcggcggca tagggatcga gccaggtctc cctgccgcgg tccaccgcgt cgcagaaccc
    22621 ctggtaggcg acgcggaata cttcctgcca ctgccgccgg tccatgcccg catgcagcgg
    22681 cggcatgccg tcggcctcgc cgttggccat gtcgagcttg tgcgcgaact cgtggatcac
    22741 cacgttcatg tccggcgcgt tgcgcgcggc gtcccaggac agcaccaccg gcccgccccg
    22801 ccaggactcg ccggcgcgct cgtcgtcgaa ctcgtgcacc acgccgtggt cgtctgtttc
    22861 cgtgaccttc accttgaagt cgccggggtg caccacgatc tcgctccaga cgtcgtagcg
    22921 gtccagcccg aggttgagca ccggcaggca ggcctgcacg gcgatggaca ggcgcatcgc
    22981 gtcgtcgagc tcgaggccgt gcgtgccgga gaattccttc tccgccagca gcagcagggc
    23041 cagctcgcgc aggcgctgcg cgtcctgcgg cggcagcccg cgcaggaacg gcagcgcgct
    23101 ggtgaccgcg cgccacagcg cctcgtcgat gcgccggcgc gcgagcaggc gccggcggcg
    23161 ccaggtcctc agccaaccca tcggatcccc ggcatggtcc tcgctacaat acctgcatgg
    23221 tctcggtcgt gcagttgcat ccgcaaaaca gcgccgccgc gctggcgcct ctcgccgcgg
    23281 gcctcgcgcc cgaggcgcag gcgcgcctcg ccgacgcgct ggcgttcgcc gagccgctgt
    23341 acgccggcca gacgctgtcc accggcgagc cggtatggcc gcacgcgctc ggcctgggcg
    23401 cgagcctcgc cgccatcggc gccgacccgg cggcgcgcgc cgccggcgtg ctgttcgccg
    23461 cgcccaagtt cctcggcgcg ggcgaggcgc tcgccgcgcg cttcggcgcc gagatcgccg
    23521 gcctggcggc gggcgtggag aagctctacc agctgcgcgt gctgacgcgc accagcgccg
    23581 cgcagcaaag cgagacattg cgcaagatgc tgctcggcat ggtggaggac gtgcgcgtgg
    23641 tgctgattcg cctggcgagc cgcacgcaga cgctgcgctg gttcgccagg aatccgggcg
    23701 aggagcgcgc cgcctacgcg cgcgagacgc tcgacatcta cgcgccgctc gcgaaccgtc
    23761 tcggcgtatg gcagctgaag tgggagctgg aagacctgtc gttccgcttc ctcgagccgg
    23821 agacctacca gcgcatcgcg aagatgctcg atgagcggcg cgtggagcgc gagcagtaca
    23881 tcgagagcgc cgtcgcggcg ctggcgcgcg agctcgcggc gggcggcgtc agggccgagg
    23941 tgagcggccg gccgaagcac atctactcca tctggaacaa gatgcgccgc aagggcctgg
    24001 acttcgggca gctgcacgac gtgcgcgccc tgcgcgtcat cgtgccggaa gtgaaggact
    24061 gctacgccgc gctcggcgcg gcgcacaacc tgtgggcgcc gatcgcgcgc gagttcgacg
    24121 actacatctc gcgcccgaag gacaacctgt accagtcgct gcacaccgcg gtcgtcggcc
    24181 ccggcggcaa gacgctggag gtgcagatcc gcaccgagga gatgcaccgc cacgccgagc
    24241 tcggcgtggc cgcgcactgg cgctacaagg aggccggcag ggcgacgcgc gccgacagca
    24301 gcttcgacga caagatcgcc tggctgcgcc agctcctcgc ctggcgcgac gaggtggccg
    24361 acagcggcag ctgggccgag aagacgcgcc gcgcggcgct cgacgacacc atctatgtgg
    24421 tgacgccgca gggcaaggtg gtggacctgc ccaagggcgc cacgccggtg gacttcgcct
    24481 acgcgctgca caccgacatc gggcaccgct gccgcggcgc caaggcggac gggcgcatcg
    24541 tgccgctcga cacgccgctg cacagcggcc agcgcgtgga gatcaccgtc gccaaggcgg
    24601 gcgggccgtc gcgcgactgg ctgagcgcgg agcgcggctt cctcaccacc agccgcgcgc
    24661 gcgccaaggt gcgccactgg ttcaacctgc aggagggcgg cagggagccc gcggccccgt
    24721 tgcggccgaa gctcgcgccg ccgccggcgc ccctcgcgcc gaagaagaag gcggcggccg
    24781 gcggcggcat cctggtcgtc ggcatggacc gcctgatgac gcagctcgcc aaatgctgca
    24841 agccggtgcc gcccgatgcg atccgcggct tcgtcacgcg cgggcgcggc gtgtcgatcc
    24901 atcgcgccga ttgcgccagc ctgcagcgcc tggcggaaaa ggacgcggcg cgcctgatcg
    24961 aggcgcagtg gggcggcgcg ccgggcgcct acggcgtgga catggtggtg acggcgagcg
    25021 accgccccgg gctgctgcgc gacatcggcg acgcactggc gcgcgagcgc atcaacgtca
    25081 ccgcggtgcg cacgcagagc cgcgacgagc tggcgaccat gcgctttgcc ttcgccattg
    25141 ccgacctcgg gcagctgaag cgcgccttcg ccgtggtgcg cgaggtgccg ggcgtgctgc
    25201 gggtggcgcg gggctgagcg cggctacagc cgcggcagca gcaccagcag ccacaccacg
    25261 cccgccgccg ccagcgcgag catgacggcg gcgctgccgt agtccttggc gcgcctggcg
    25321 agctcgtggt cctccagcga cacgcggtcc accgccgctt cgaccgcgga gttgagcagt
    25381 tcgaccaccg gcaccagcag cacgctgccg gcaagcaggg cgcgctcgac gccgtcggcg
    25441 ccgagccaga tgccgagcgg caccaggatt gccgcgagca gcagttcctg gcggaacgcg
    25501 gactccagtc gaacggccgc ggccagcccc gcgagcgagt accccgtcgc atcgagcagg
    25561 cgccgcatcc cggttgctcc cttgtacggg ctcttggcgt ccatcgcggc caatctagca
    25621 cgcgcctaca gcaggtccgg cgccagcacg gcgtgcacca ccgccgccgc gccctcgccg
    25681 cgcgggtggg acctgagcca ccacagtccg cccgccgcga tcgagccgcc gcccgccgct
    25741 ccggcggcaa ggcgcagcgc cgcgcggccc aggtccggct ggtggccgac caccaccacc
    25801 gcgcgcccgc cgccgggcca gccggcggcg tcgaggatcg cctcgagcgg ggcgcccggc
    25861 gcgaggcccg gcaccgtctg cggttcggcg cagagcgcgc gcgcggtttc ctgggcgcgg
    25921 cgcgctgggc tcgccagcac gacgaattcc gccggcaggc gctcgcgcag ccacgcggcg
    25981 acgcgcgccg cgtcctcgcg gccgcgcggc gtgagcccgc gctccaggtc gggcgcgccg
    26041 gccgcggcct cggcatggcg ccacaggatc aggtccatct tcagttaaaa tcccgcattc
    26101 gttccatcag gcgcgtagct cagctggtta gagcactacc ttgacatggt aggggtcgtt
    26161 ggttcgagtc caatcgcgcc taccaataac ttgcccaaca tcaagctccc agacggctcg
    26221 gtgaagacct tcccgggccc ggtcaccgtc gccgaagtgg cccagtccgt cggggcgggg
    26281 ctggcgcgcg cggcgctcgc cggcagggtc gacggcaggc tcgtcgacac gagcttccgc
    26341 atcgagggcg acgccgccct cgccatcgtc accgaccgcg acgccgaggg cctggagatt
    26401 atccgccatt ccaccgcgca tctgctggcg caggcggtga agcagctgta ccccgaagcg
    26461 caggtgacca tcgggccggt gatcgagaac ggcttctact acgatttttc ctacaagcgg
    26521 ccgttcacgc cggaggatct ggcggcgatc gagaagcgca tgggcgagat cgcgaagcag
    26581 gacctgccgg tcgagcggcg cgagatgccg cgcgacgaag cagtgaagta cttccgcggc
    26641 ctgggggaga tctacaaggc ggagatcatc gcctccatcc cggccaagga ggcgatctcg
    26701 ctctacggcc agggcggctg ggtcgacctg tgccgcgggc cgcacgtgcc ctccaccggc
    26761 aagctcaagg tgttcaagct gatgcgcgtg gccggcgcct actggcgcgg cgactcgaag
    26821 aacgagatgc tgcagcggat ctacggcacg gcctgggcga agaaggagga ccaggacgcc
    26881 tacctgaaga tgctggagga ggccgagaag cgcgaccacc ggcgcctggg cacgcagctc
    26941 gacctgttcc acatggagga ggaggccccg ggcatggtgt tctggcatcc caagggctgg
    27001 acgctgtggc agcaggtgga gcagtacatg cggcgcgtgt accgcgacgg cggctacctg
    27061 gaagtgcgcg ggccgatggt gctgccggtc agcctgtgga agatcaccgg gcactgggag
    27121 aactaccgcg agcacatgtt caccaccgaa tcggagaagc gcgactacgc gctgaagccg
    27181 atgaactgcc cgggccacgt gctgatctac aacaagggcg tgcgcagcta ccgcgacctg
    27241 ccgctgcgct acggcgagtt cggttcctgc caccgcaacg aggcctcggg cgcgctgcac
    27301 gggctgatgc gcatccgcgg tttcacgcag gacgacggcc acatcttctg cacgcaggag
    27361 cagatcgtgc ccgagtgcgt ggcctacacc gcgctggtgc agaaggtgta cgcgcatttc
    27421 ggcttcaagg acatcgtcta caaggtggcg acgcgcccgg agaagcgcct cggcaccgac
    27481 gagcagtggg accgcgccga gggcgcgctc aagctgtcgc tggaggaggc gggcgcgcgg
    27541 tacgagatcg cggcgggcga cggcgtcttc tacgggccga agatcgagta ccacctcaag
    27601 gacgcgatcg gccggcagtg gcagtgcggc accatgcagg tggacttcca gctgccggtg
    27661 cgcctgggcg ccgagtacgt cgccgccgac aacacccgcc aggtgccggc gatgctgcac
    27721 cgggcgacga tcggctccat ggagcgcttc atcggcatcc tgatcgagca ctacggcggc
    27781 gccttcccgc tgtggctctc gcccgagcag gtggccgtgc tgaacatctc cgagcaccag
    27841 gcggactacg ccaaggccgt ggccgcggcc ctgcgccagg ccggttttcg cgtcgtggaa
    27901 gatttgcgaa acgagaaaat ttcctataag attcgagaac atagcttgca aaagctcccc
    27961 taccagctgg tggtgggaga caaggaaaag caggctggat cggtggccgt gcgcacgcgc
    28021 ggcggtgaag atctcggcgc gatggcgctc gatgacttca tcgcacgcct ccgcaacgag
    28081 gcgagcgcgt aggcagcacg gccggttttt ttgacgagcg taaggagctg tcacccatcg
    28141 cactcgaaag atcgcagcgc atcaacgggg agatcaacgc accgcaggtg cgtctggtcg
    28201 gtgagcaggg agagcagcac ggcatcgtgg cgattgccga tgcgctgcgc atggcggaag
    28261 agcagaacgt ggacctggtg gagatcgcgc cgctcgcggt gccgccggtc tgcaagctga
    28321 tggactacgg caagttccgc taccgcgagc agaagaaggc gcacgaggcc aagctcaagc
    28381 agaagcagat ccaggtcaag gaaatcaagt tccgccccgg caccgacgag ggcgactaca
    28441 agatcaagct cggcaagctg acgcagttcc tggaagacgg cgacaaggcg aaggtgacgc
    28501 tgcgcttccg cggccgcgaa atggcgcacc aggaattcgg cgtgcggctg ctggagcggg
    28561 tgaaggcgga cctggagaag gtgggcctgg tggagcagtt ccccaagctc gaaggccgcc
    28621 agatggtgat ggttctcgcg cccaagaaga agggcgtgcc gcccaaggcg aggccgaagg
    28681 cggacaagga gcccagggaa gcgaaggaag cgaaggccaa gaaggaaagc gcagcaaaac
    28741 ccgccgcggc gacctagcgg tcgccgcgcg caacaagtga tggccgcggc ttcaagcgcc
    28801 cgcaaggcgg gccgcccggc gtcaggctaa aacagaagga gcagtcccgt gcccaaggta
    28861 aagaccaaga agtccgcggc gaagcgcttc aagatccgct cctccgggag catcaagcgc
    28921 tcgcaggcct acatgcgcca catcctcacc aagaagtcga ccaagcggaa gcgccacctg
    28981 cgcggcacca ccgaggtcca cgccaccaac aagcgggcag tgcgcgccat gctgccctac
    29041 tcctcgtagg gggccgccat gccgagagtc aaacgcgggg tcacggcgcg cgcgcgccac
    29101 aagaaggtac tgaagcaggc caagggtttc cgcggccgcc gcggcaacgt gtatcgcgtc
    29161 gccaagcagg cggtgatgaa ggcggggcag tatgcctacc gcgaccggcg caaccgcaag
    29221 cgcaccttcc gcgcgctgtg gatcgcgcgc atcaacgccg cggtgcgcga gctgggcatg
    29281 agctacagca ccttcatggc cggcctgaag cgcgccaaca tcgagatcga ccgcaaggtg
    29341 ctggcggatc tggcggtggc ggacaagccc gcgttcgcca ggatcgccag ccaggtcaag
    29401 gccagcctcg cccactagat tcctcccggg cccgcccatg gaggagctgg agagcctcgt
    29461 cggcagcgcg cttcgcgagt ttggcgaggc gcgcgacgcg cccgcgctcg aggatgcgaa
    29521 ggcgcgctac ctcggcaagt cgggcgcgct gaacgccttc cggcccgcgg gcggaacgcc
    29581 agaggagagg aaggctgcgg gcgccgcgta caacgccgcc aaggagcgca tcgagcgtgc
    29641 gctggctgcg cgccgcgccg agctcgccgg cgcgcagctc gaatcgcgcc tcgccgagga
    29701 ggcgctcgat gtgacgctgc cggcccgcgg ccgcggccgc ggcggcatcc accccatcat
    29761 ccgcacctgg cagaggatcg aggcgctgtt cggctcgatc ggcttcgcgg tcgccgacgg
    29821 cccggagatc gagaccgact ggtacaactt caccgcgctc aacaatccgg agaaccatcc
    29881 ggcgcgctcg atgcaggaca ccttctacgt cgacctgaag gacgcgcagg gactgccgct
    29941 tctgctgcgc acacacacga gtcccatgca ggtgcgctac gcgaggatgc acaagccccc
    30001 catcaaggtg atcgcgccgg gccgcaccta ccgcgtggat tccgacgcca cgcactcgcc
    30061 gatgttccac caggtcgagg gactgtggat cgacgagggc atcagcttcg ccgacctgaa
    30121 gggcgtgtac gtggacttcc tgcggcgctt cttcgaatcg gatgacctgc aagtgcgctt
    30181 ccgcccttcg tacttcccgt tcaccgagcc ctcggccgag atcgacctgc agttcgccag
    30241 cgggcccctg caggggcgct ggctggaact ctccggcgcg ggccaggtgc atccgcaggt
    30301 ggtgcgcaat ttcggactgg acccggagcg ctataccggc ttcgcgttcg gctcgggcct
    30361 ggagcgcctc gccatgctgc gctacggcgt cgacgacctg cggctgttct tcgacggcga
    30421 cctgcgcttc ctcaggcagt tcgcatgaac gtccccgaga gctggctgcg cagcttctgc
    30481 gacccgtcgc tgtccgggcg gcagctcgcc gacaagctga cgatggcagg gctggaagtc
    30541 gaaggctacg cgccggtcgg ggcgcagctg cagggcgacg tcgtggtggc cgaggtgctc
    30601 tccgtcgaga aacacccgaa tgccgacaag ctcacggtgt gccgcgtgcg cgccggccgc
    30661 gacacggtgc aggtggtctg cggcgcaccc aacgtgcgcc ccggcatgaa ggcgccgctg
    30721 gcgcgggtcg gcgtgaaagc ggtgcgcggg atcgagagcc acggcatgct ggcctcggcg
    30781 agggaactcg ggctgtcgga agaccattcc ggcatcctgg aactgcccgc ctcggcaaag
    30841 ccgggcagcg acgcgcgcaa gctgctcggc ctcgacgagc acgtgatgac gttcaagctg
    30901 acgccgaacc ggcccgactg cctgtcggtg ctcggcatcg cgcgcgaggt gtccgcgctc
    30961 accggcgcga agctcacggc gccggcgatc aggccggtcg cggcggaaag caaggcgaag
    31021 caccccttcg gcatcaccca tgccgagggc tgcgggcgct tcgccggccg cgtgatccgc
    31081 ggcgtggatg cggcggcgcc cacgccgggg tggatgcgcc agcgcctgga gcgagccggc
    31141 cagcgctcga tctcggcgct ggtggacgtc accaactacg tgatgctcga gctcggccgg
    31201 ccgctgcacg tctacgacca ggacaagctg cgcggcgcga tcgacgtgcg ctggggccgc
    31261 aagggcgaaa aggtcctgct gctgaacggc gaggaagtcg aggtggaccc cgcggtgctg
    31321 tgcatcgccg acgactccgg accgatcggc ctcgccggca tcatgggcgg ggagtccacc
    31381 aaggcggaaa cctcgacgcg caacctgttc ctcgagtcgg cgttcttcca ccccgaggcg
    31441 atcgccgggc gcgcgcgccg ctacaacttc gccagcgacg cctcgcaccg cttcgagcgc
    31501 ggcgtggatt tcgccaacaa cgtcgacggc atcgagcgcg cgacgcagct gatcctggaa
    31561 atctgcggcg gcgagcccgg cccgacggtg gacctggtga agcgcctgcc gaagaggaac
    31621 ccggtgcgca tgcgcgcctc gcgcgcgcag aaagtgatcg gcatcccggt gtcggaaaag
    31681 gagatgggcg gggtgttcaa gcgcctgggc ctgcccttca aggcgaaggc gggccgcttc
    31741 accgtcgcgc cgccttcgtt ccgcttcgac ctgcagatcg aggaggacct gatcgaggag
    31801 gtggcgcggg tgcacggctt cgagcgcatc cccgcgcggc cgccgcgcgt gccggcggcg
    31861 atggccgccc tgcccgaagc gcggcgctcg ctgcacgccc tgcgcgagcg cctggcggcc
    31921 tgcgactacc aggaagtgat caacttcagc ttcgtcgagc cgcagtggga ggcggacctg
    31981 gcgggcgagg cgaaccccgt ccggctgctc aacccgatcg cgagccagtc gtcggcgatg
    32041 cgcaccacgc tgctcggcgg actgctggcg aacctgcgct acaacgcggc gcgcaagctg
    32101 tcccgcatcc gggtgttcga ggtcggccgg gtgttcctgc gcgatccatc ggtcaaggac
    32161 ggccccctgg aagtggccgg gctgcgccaa cccatgcgca tcgccgctgc cgcctgcggc
    32221 ccggccctcg aggagcagtg gggtgccgtg gcccggcagg tcgatctctt cgacctcaag
    32281 gccgacgtcg aagccctggt attcccccgg gcggccagat ttgaagccgc agcccacccg
    32341 gcgttccatc cgcagcgttc ggcgcgcatc cgggtggagg gcagggaggc cggctggatc
    32401 ggtgagctgc atccgcgctg gcaacagaaa tatgaaataa atcaaagtgt tattatattt
    32461 gacgtcgatg ccggagtgct ccaggacatc gccttgccgc aggcctcgat cccatcccgg
    32521 tttcctccag tgactcggga catcgccttg ttagtgaacg ctgacaccga agtccaggcc
    32581 ctgctcgacg cggtttccga gggaaagcca gccatcgtgc gggaggttcg gctgttcgac
    32641 ttgtaccaag gcccgaacct tcccgctggc agaaaaagcc ttgcgttccg ggtagttatg
    32701 caagatactg aacgaaccct cacggatgcg gaggccgatt cggcgcttca ggcgctagtc
    32761 gagctgtggg gacgtcgttt tggcgcgaca cctcgcacat aaataaagaa atcagggagt
    32821 tgtgatgact ttgaccaagg ccgaactggc cgacctcctg ttcgagaagg tcggcctgaa
    32881 caagcgcgag gccaaggaca tggtggaagc cttcttcgag gagatccgcg gggccctcga
    32941 gcgcggcgag agcgtcaagc tctcgggctt cggcaatttc cagttgcgcg acaagccgca
    33001 acggccgggg cgcaacccca agaccggcga agagatcccg atcagcgccc gccgcgtggt
    33061 caccttccac gccagccaga agctgaaggc gatggtggaa aaggcgcgcc atggaaagcc
    33121 gcaaccccag caagaaagct gagctgccgc cgatcccggc gaagcgctac ttcaccatcg
    33181 gtgaggtgag cgagctgtgc ggcgtcaagc cgcacgtgct gcgctactgg gagcaggaat
    33241 tcacccagct caagccggtc aagcgccgcg gcaaccggcg ctactaccag caccacgaag
    33301 tgctcctcat ccggcgcatc cgcgagctgc tgtacgaaga gggcttcacc atcagcggcg
    33361 cgcgcaaccg gctggacaac gcgctcggca gcgaggagcg cgccgagccg gcggggcgcg
    33421 ccgccgccgg caacggcacg ggcgccgacc tctcggcgat caagcgggag ctgcgcgcgc
    33481 tgctggagac gctgcgggct tagaattcag gcacgcgggg ttgccggggg gcgccggcgg
    33541 aggcaggacg gcgacatgcg cttcacattg gccgcggagc aggatttcct gagggccgag
    33601 ctgttcgagc gggaaaccgc ggcgcagacg cgtgaattcc tcgcggccgc cgccgccgag
    33661 gcgctgcggc tgggactcag caaggcgctg atcagcgtgc acgcgtcgcg caccatcttc
    33721 cgggtcgagc agtaccagtt ctcggcctac gtcaaggcgc tcgcggaacg gccctcggcc
    33781 atggtcgcgc tggtggccgg cagcgacgaa atccacgccg cgcaccagta cgtcgagctg
    33841 ctggcccagc agcacggggc caacgtgcgc tgctttcgcc acgaggcgga agcggtccag
    33901 tggctgcgaa gccggatata atccgccgct cggggcgtag cgcagcctgg tagcgtacct
    33961 gcatggggtg caggtggtcg cgagttcgaa tctcgccgcc ccgaccaaaa ttccctcatg
    34021 ctgcaagaca aagtcgtagt gatcacgggc gcgggcggcg gaatcggccg cgacttcgcg
    34081 ctcgcgttcg ccgcgcacgg cgcgcgggtg gtggtcaacg acctgggcac ctcggtggcg
    34141 ggcgagggcg cgagcagcgg cccggcgcag aaggtggtgg acgagatcaa gtcgcagggc
    34201 ggccgggcgg tggccagcac cgacagcgtc gccgagtggg aatcggccaa ccgcatcgtc
    34261 aagaccgcca tcgacgcctt cgggcgcatc gactgcgtgg tgaacaatgc cggcatcctg
    34321 cgcgaccgct ttttcttcaa catgtcggtc gaggaatgga aggcggtgat cgacgtgcac
    34381 ctgaacggcg cgttctacgt ggcgcgcgcc gccgcgccgt acttcaaggg ccagaacgcg
    34441 ggctgctacg tcaacatgac ctccacctcg ggactgatcg gcaacctcgg ccaggccaac
    34501 tactcggcgg ccaagctcgg catcgccgcg ctgtccaagt ccatcgccct cgacatggcg
    34561 aagtaccggg tgcgctccaa ctgcatcgcg ccgttcgcct ggagccgcat gatcggctcg
    34621 atccccaccg agaccgccga gcagaaggcg cgcgtggaga agatgaaggt catgcaaacg
    34681 gcgaagatcg cgccgctcgc ggtgtacctg gcgagcgacg ccgcgcagga cgtctccggc
    34741 cagatcttcg cggtgcgcgc caatgagatc ttcctcatgt cgcagagccg gccggtgcgc
    34801 tcggtgcacc gcgccgaggg gtggacgccg cagaccatcg gcgcgcaggc gatgccggcg
    34861 ctgcgcggca gctttttccc gctcgaccgc acgcaggacg tgttcacgtg ggacccgctc
    34921 tagccgacgc cggccacgcc gagctgcgcg cgggcgtgcg cggcctgtgc gcgcagttcg
    34981 actccgccta ctggcagaag gtggacgagg cgcgcgccta ccccgaggct ttcgtcgacg
    35041 cgctcaccgc ggccggctgg atgtcggcgc tgatcccgga gcagtacggc ggctcggggc
    35101 tctcgctcac gcaggccacg gtgatcatgg aggagatctc gcgctccggc ggcaatcccg
    35161 gctgctgcca cggccagatg tacaacatga acacgctgct caggaacggc tcggaggcgc
    35221 agaagaggaa gtacctgccg aggatcgcct cgggcgagct gcgcatgcag gcgatggggg
    35281 tcaccgagcc cagcgccggt accgacacca cgaagatcaa gaccgtggcg gtgaagaagg
    35341 gcgggcgcta cgtggtcaac ggccagaagg tctggacctc gcgcctgcag cactcggacc
    35401 tgatgatcct gctggcgcgc accacgccgc tcgccgaggt gaagaagaag tccgagggca
    35461 tgtcggtgtt cctggtggaa aaggcgcagg cgctcggcaa gggcctgagc atgcggccga
    35521 tccgcaacat ggtgaaccac gagaccaacg aggtgttcct cgacaacctc gagatcccgg
    35581 ccgacaacct gatcggcgag gagggccagg gcttcaggta catcctcgac gggctgaacg
    35641 cggagcgcat cctgatcgcc gccgaatgca tcggcgacgg ctactggttc accgagcgcg
    35701 cggtgaagta cgccaaggag cgcgtggtgt tcgaccggcc gatcgggcag aaccagggcg
    35761 tgcagttccc catcgccgag tgccacatgg aggtcgaggc ggccaacctg atgcgctaca
    35821 aggccgcggc cctgtacgac gccaagcagc actgcggcgc cgaggccaac atggccaagc
    35881 acctcgccgc caaggcctcc tgggaggcgg ccaacgtctg cctgcagacg cacggcggct
    35941 tcggcttcgc cgcggagtac gacatcgagc gcaagttccg cgagacgcgc ctgtacctgg
    36001 tggcgccgat ctcgacgaac ctgatcctgt cctacgtcgc cgagcacgtg ctgggactgc
    36061 cgcgatcgtt ctagggccgg tgcgtcgccg tcggctagaa tcgcgcaggc gatgcgcatc
    36121 ctggtttcca acgacgatgg ctatttctcc cccgggatcg cgctgctggc cgagcagctc
    36181 gccccgctgg gcgaggtcac ggtggtggcg cccgagcgcg accgctccgg cgcctcgaat
    36241 tccctgacgc tcgaccggcc gctgtcggtg cggcgcgcgc cgaacggctt cctgtacgtc
    36301 aacggcacgc ccaccgactg cgtgcacctc gcggtcaccg gcctgctcga cgagctgccc
    36361 gacatggtgg tgtcgggcat caatcttggc gccaacatgg gcgacgacac gatctactcg
    36421 ggcaccgtcg ccgcggcgat ggaggggttc ctcctcggca tcccgtcgct cgccgtgtcg
    36481 ctcgccagcc gcgaggcgca gcacctgcaa tcggcggccg cggtggcgcg cgggctggtg
    36541 gagcgcttcg ggcgcgcgcc cttcggccag ccggtgctgc tcaacgtcaa cgtgcccgat
    36601 gtgccgccgc aggcgctcgg cgcgatcgag gtcacgcgcc tggggcgccg ccacaaggcc
    36661 gagccggtgg aaaagacgcg cacgccgcgc ggcgagacgg tctactggat cggcgccgcc
    36721 ggcgcggccg cggacgcggg cccgggcacg gacttccacg cggtggcggc gcgccgcgtg
    36781 tcggtgacgc cgctgcaggt ggacctgacg caccacgcgc acctcggcat gctgcaggac
    36841 tggctcgcgc ggtgagcgcg cgcagcgccg ccggcgtcgg catgacctcg cagcgcacgc
    36901 gcgcgcgcat gctcgagcgg ctgcgcggcg agggcatccg cgacgagaac gtgctcgcgg
    36961 cgatgggcag cgtcccgcgc cacctgttcg tcgacgaggc gctggcatcg cgcgcctacg
    37021 a
//
LOCUS       DASRIE010000026         7291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1586940, whole genome shotgun sequence.
ACCESSION   DASRIE010000026 DASRIE010000000
VERSION     DASRIE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586940"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFB53_01285"
     CDS             complement(<1..829)
                     /locus_tag="VFB53_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HZN85392.1"
                     /translation="MPVPADARRALAAGWLLLALGALLASGVFSILLVLSRAPYVKDV
                     FPLVDFFRVALVVHVDLSVLVWFSAFAGLLWSLHGAPRVLWLGWAALAGASAGALGMC
                     AAPFLGSGAPLMSNYIPVLDSPPFLRALALFGVSCALLVARALAVAPRVGIRPDGSAA
                     LRFGLNAAAISAAVALLAFAWSYAEVPANIERKAYFEVLFWGGGHVLQFTWTLLMFAA
                     WLWLAEATGARTPLSPRVAILLFAIALVSVFATPAIYLAYDVVSVQHYRTQTWLMRIG
                     "
     gene            complement(861..2129)
                     /locus_tag="VFB53_01290"
     CDS             complement(861..2129)
                     /locus_tag="VFB53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZN85393.1"
                     /translation="MRHLGALGFLLFWLLAASGLYLYLRFDTSAAGAYRSIGELEWWF
                     GGVLRSLHRYAADAFVAVTLLHLLRELYLGRFRGWRAFTWISGIPLVWLLYASGLIGY
                     WLVWDSRSQFSFLATMEWAGVAARAGEAPDRLFSLFVFLHIGLPLALLAGMWVHVQRL
                     GLPKTYPPRGLGFAVAGMLLTLALLRPAVSEPRWDAARAAQDVAVDWFYQFIHPLMYA
                     TSPAFLWAAVLGASALLLALPYALRKRVVPAQVSPANCNGCGRCVADCPYQAVILSGG
                     KAAVFPERCAACGICAGACPSSTPFRSIRDLVSGIELPELTTGVLREKLNDALALRTG
                     EIAFCCEKNPVPGAISLRCLGMLPPAFVEYALRHGAARVKALGCKGECAYRLGLELSE
                     ERFARRREPHLRATVRCERDNHEYRFVLDR"
     gene            complement(2126..2629)
                     /locus_tag="VFB53_01295"
     CDS             complement(2126..2629)
                     /locus_tag="VFB53_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85394.1"
                     /translation="MKALRIAAQLALYAPLMAILAYFSTEPRFSVVAPGEALVRVSLI
                     HATQRREACRERSAEELAKLAPNMRASLDCPRERAPLLVEVELDGKLVLRREAQPSGL
                     RRDGNAAVYERFALPAGRHRIVARLRDRPEGGFGYVKDETVELAAGRVLIIDFNAAKG
                     GFAFHAG"
     gene            complement(2626..4521)
                     /locus_tag="VFB53_01300"
     CDS             complement(2626..4521)
                     /locus_tag="VFB53_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZN85395.1"
                     /translation="MLGRIQAALQWGFTKVEGLFNLAFGEKLNPLYYIGPISYYLMWL
                     IVASGLYLYAFFETSVAGAQSSVEALTHGQPWAGGVLRSVHRYASDGVVLTMLLHMAR
                     HWAFDRYRGFRWFSWVSGLVVLWLVYVAGINGYMLPWDRLAKFTVIATAEWLDWLPLF
                     NGTLVRNFIFEENVNDRLFSLLSFIHIGVPLALLAILWVHTQRVPQARTSPPLPIMLT
                     LTGALIVLSIAKPATSQISAQNELGFDWFYLPVLALLYRWAPGEVWLLVGAGSALLFL
                     LPWLPPKRRGAERYMLVRPDNRIIAVRAGETLLDAGLREGIALPFDCRSGGCGECKAT
                     LRAGSVDFGAYQHGVLSAAERAAGTILPCVCTARDDVELEYRGSAAAAAARAWSATVE
                     SVQRLAPDVMQVLLKLDGGERIAFYAGQYINILLEDGEKRSFSFATAPAVNDRIELHI
                     RRIPGGRYTAHVFERMRAGERVRFEGPLGSFFLREESDKPMIFVAGSTGFAPVKSMLE
                     YAFARGVKRRMVLYWGVRRPADLYLRELPERWAREHPNFSFVPVISHPAPEDAWAGRT
                     GLVHQAILADFPDLAGFQVYACGSAAMVQAAHPAFSAQGLGPDDCFSDAFVARRAPEM
                     VQLGGTA"
     gene            complement(4531..5328)
                     /locus_tag="VFB53_01305"
     CDS             complement(4531..5328)
                     /locus_tag="VFB53_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZN85396.1"
                     /translation="MWRFSLSFLLFLGNAHAFDEKAALQDSQAALGRQIGDYRLIDSD
                     NRRVSLAELRGKPLVVHFVYTGCFQVCPATTQFLASAVREAERTLGPGTFRVATIGFN
                     LPFDDPGSMRAFQKKAGVGSPHWLFLTPEAGTVAALTREFGFRYEATAAGFDHLLQVS
                     IVDGEGRIYRQLYGDSFDSPRFVGPLLELARNAPVAQPSLETAWEKFKLLCTVYDPAA
                     GRYRLNYVVLIEILVGTSVMLGGIGFVLREWRRRRLTHIKSTLEPSP"
     gene            complement(5309..6166)
                     /gene="cyoE"
                     /locus_tag="VFB53_01310"
     CDS             complement(5309..6166)
                     /gene="cyoE"
                     /locus_tag="VFB53_01310"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN85397.1"
                     /translation="MQVRAFVNLFKLRIGVAIALAAAAGLAVEQGSAGFFQGLVLVLA
                     VLVASAAAGAFNQYVERDIDVKMARTRNRPFVTGALRHGPFWLWVIGAMTAGSVLAAA
                     LAANFLAGLYVFLGAFTYGVVYTVWLKRRTWCNIVIGGLAGSFAVLAGAAAVSPAPGP
                     EALALAAVLFLWTPPHFWSLAIACREDYAAAGVPMLPVVVGDARAARAVHAAAGLLVA
                     ASVVPFFFGLGALYLGAALAGGAYFLAKSLALMRAPGRATARAAFLASLVQLCAVLFG
                     AMADVALLS"
     gene            complement(6170..>7291)
                     /locus_tag="VFB53_01315"
     CDS             complement(6170..>7291)
                     /locus_tag="VFB53_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN85398.1"
                     /translation="GAIILIPTFLWSVGLVEYIDSLMYKTIWWAFGHSSQQVNVAAHV
                     SVWYAIAAIVFGAKPMSEKVSRTAFLLYIFFLQLASAHHLLTDPGLTSNWKIFNTSYA
                     MYLAVLASMVHGLTVPGAIEVAQRKKGYTQGLFEWLRKAPWGNPVFSSMFISLVGFGF
                     LGGISGVMMGTEQLNLIIHNTIYVPGHFHATVVIGTTLAFMGLTYFLIPVLFRRELAF
                     PTLAKYQPYVFGLAMSGVALFLMGAGTLGVPRRHWDMGFAGNAFPYEFPGAAWLMMAL
                     AGFSGLIAVVGGGMYLLITVWSVFLGKRLDRPGFLHVRARDGMASAPAAGSGAVLTGH
                     GSAGMVAPGTFVLALVFLTSFVLYYFVNWKYLSTVWPLK"
BASE COUNT         1305 a         2507 c         2609 g          870 t
ORIGIN      
        1 cgccgatgcg catcagccag gtctgcgtgc ggtagtgctg gaccgaaacc acgtcgtagg
       61 cgaggtagat cgccggcgtc gcgaacaccg agaccagggc gatcgcgaac agcaggatcg
      121 cgacgcgcgg cgaaagcggg gtgcgcgcgc cggtcgcctc ggcgagccac agccatgcgg
      181 cgaacatgag gagcgtccag gtgaattgca gcacgtggcc gccgccccag aacaggacct
      241 cgaagtaggc cttgcgctcg atgttggcgg gcacctcggc gtacgaccag gcaaacgcaa
      301 gcagcgcgac cgccgccgag atcgccgcgg cattgaggcc gaagcgcagc gcggccgagc
      361 cgtcgggccg gatgcccacg cgcggcgcca ccgcgagcgc gcgcgccacc agcagcgcgc
      421 acgagacgcc gaagagcgcg agcgcgcgca ggaacggcgg gctgtcgagc accggaatgt
      481 agttgctcat cagcggcgct ccggagccga ggaacggcgc ggcgcacatc cccagcgcgc
      541 cggcgctcgc gccggcaagc gccgcccagc cgagccagag cacgcgcggc gcgccgtgca
      601 ggctccacag cagcccggcg aacgccgaga accacaccag cacggacagg tccacgtgca
      661 ccacgagcgc gacgcggaag aagtccacca gcggaaagac gtccttcacg tacggcgcgc
      721 gcgagagcac cagcaggatc gagaacactc cggaggcgag cagcgcgccg agcgcgagca
      781 gcagccagcc ggcggcgagc gcgcggcgcg cgtccgccgg caccggcaag gcgtacgaaa
      841 aaggggtcag gacccttttc tcagcggtcc aggacgaagc gatactcatg gttgtccctt
      901 tcgcagcgca ccgtcgcgcg cagatgcggc tcgcgccggc gcgcgaagcg ctcttcgctc
      961 agctccaggc cgagccggta ggcgcattcg cccttgcagc cgagcgcctt cacccgcgcg
     1021 gcgccgtgcc gcagcgcgta ctcgacgaag gcgggcggca gcatgccgag gcagcgcagc
     1081 gagatcgcgc ccggcacggg gtttttctcg cagcagaacg cgatctcccc tgtgcgcaga
     1141 gcgagcgcgt cgttcagctt ttccctcagc acgcccgtgg tgagctccgg cagctcgatg
     1201 cccgagacga gatcgcggat gctgcggaac ggcgtcgagg acgggcaggc gccggcgcag
     1261 atcccgcacg cggcgcagcg ctccgggaat accgccgcct tgccgccgga aaggatcacc
     1321 gcctggtacg ggcagtccgc cacgcagcgg ccgcagccgt tgcagttcgc ggggctcacc
     1381 tgcgcgggga ccacgcgctt cctcaaggca tagggaagcg cgaggaggag cgccgatgct
     1441 cccagcacgg cggcccagag gaacgcaggc gaggtcgcat acatcagcgg gtggatgaac
     1501 tggtagaacc agtccaccgc cacatcctgc gccgcgcgcg ccgcgtccca gcgcggctcc
     1561 gacacggcgg ggcgcagcag cgcaagcgtc agcagcatgc ccgcgacggc gaagccgagc
     1621 ccccgcggcg ggtaggtctt cggcaggccc aggcgctgca cgtggaccca cattcccgcg
     1681 agcagcgcga gcggcaggcc gatgtgcagg aacacgaaca gcgagaacag gcgatcgggc
     1741 gcctcgccgg cgcgcgccgc gacgcccgcc cattccatcg tcgcgaggaa ggaaaactgc
     1801 gagcgcgagt cccacaccag ccagtagccg atcaggcccg aggcatagag cagccagacg
     1861 agcggtatgc cggagatcca ggtaaacgcg cgccagccgc ggaaacggcc gaggtagagc
     1921 tcccgcagga gatgcagcag cgtcaccgcc acgaaggcgt cggccgcgta ccggtgcagg
     1981 cttcgcaata cgccgccgaa ccaccactcc agctcgccga tcgagcggta ggcgccggcg
     2041 gcgctggtgt cgaagcgcag gtagaggtac aggccgctcg ccgccaggag ccagaacagc
     2101 aggaagccga gcgcgccgag gtgtctcatc ccgcatggaa cgcaaatccg cccttcgccg
     2161 cgttgaagtc gatgatcagc acgcggccgg cggcgagctc gacggtctcg tccttgacgt
     2221 agccgaagcc gccttcgggc cggtcgcgca ggcgcgcgac gatgcggtgg cgcccggcgg
     2281 ggagcgcgaa gcgctcatac accgcggcat tgccgtcgcg gcgcaggccc gagggctggg
     2341 cttcgcgccg cagcacgagc ttgccgtcga gctcgacttc caccagcagc ggcgcgcgct
     2401 cgcgcgggca gtcgagcgac gcgcgcatgt tgggcgcgag cttcgccagc tcctcggcgc
     2461 tcctctcgcg gcatgcctcc ctgcgctggg tggcgtggat cagcgaaacg cgcaccagcg
     2521 cctcgcccgg cgccaccacc gagaagcgcg gctcggtgga gaagtaggcg aggatcgcca
     2581 tgagcggcgc gtacagcgcg agctgcgccg cgatgcgcag cgccttcacg cggtgccccc
     2641 gagctgcacc atttccggcg cgcgccgcgc cacgaaggcg tccgagaagc agtcgtcggg
     2701 gccgaggccc tgcgcggaga aggccgggtg ggccgcctgc accatcgccg ccgagccgca
     2761 ggcgtacacc tggaatcccg cgaggtcggg gaagtcggcg aggatcgcct ggtgcacgag
     2821 cccggtgcgg ccggcccacg cgtcctcggg cgccgggtgc gagatcaccg gcacgaagct
     2881 gaagttggga tgctcgcgcg cccagcgctc cggcagctcg cgcaggtaga ggtcggccgg
     2941 ccgccgcacg ccccagtaga gcaccatgcg gcgcttgacg ccgcgggcga aggcgtactc
     3001 gagcatgctc ttcacgggcg cgaagccggt cgagccggcg acgaagatca tcggcttgtc
     3061 cgactcctcg cgcaggaaga acgagccgag cgggccctcg aagcgcacgc gctcgccggc
     3121 gcgcatgcgc tcgaacacgt gcgcggtgta gcgcccgccg ggaatgcgcc ggatgtgcag
     3181 ctcgatgcgg tcgttcacgg ccggcgcagt ggcgaaggag aagctgcgct tctcgccgtc
     3241 ctcgagcagg atgttgatgt actggccggc gtagaaggcg atgcgctcgc cgccgtcgag
     3301 cttgaggagc acctgcatca cgtcgggcgc caggcgctgc acgctttcca ccgtcgcgct
     3361 ccaggcccgc gccgcggccg cggcggcaga tccccgatat tcgagctcga catcgtcgcg
     3421 cgccgtgcaa acgcagggca gtatcgtgcc ggcggcgcgc tctgccgcgc tcagcacgcc
     3481 gtgctggtag gcgccgaagt cgacgctgcc cgcgcgcagc gtggccttgc attcgccgca
     3541 gccgccgctg cggcaatcga acggcagagc gatgccctcg cgcagcccgg cgtcgagcag
     3601 cgtctcgccg gcgcgcaccg cgatgatgcg gttgtcgggc cgcaccagca tgtagcgctc
     3661 ggcgccgcgg cgcttcggcg gcagccacgg cagcaggaag agcagcgcgc tgccggcgcc
     3721 caccagcagc catacctcgc cgggcgccca gcggtacagc agcgcgagca cggggaggta
     3781 gaaccagtcg aagcccagct cgttttgcgc ggaaatctga ctcgtggccg gcttggcgat
     3841 ggaaagcacg atcagcgcgc cggtgagggt cagcatgatc ggcagcggcg gcgaggttcg
     3901 cgcctgcggc acgcgctgcg tgtgcaccca gaggatcgcg agcagcgcga gcggcacgcc
     3961 gatgtggatg aacgagagga gcgagaacag gcggtcgttc acgttctcct cgaagatgaa
     4021 gttgcgcacc agcgtgccgt tgaacagcgg cagccagtcg agccattcgg cggtggcgat
     4081 caccgtgaac ttcgccaggc ggtcccacgg cagcatgtag ccgttgatgc cggccacgta
     4141 cacgagccac agcaccacga gcccgctcac ccacgagaac cagcggaagc cgcggtagcg
     4201 gtcgaacgcc cagtgccgcg ccatgtgcag cagcatggtg aggaccacgc cgtcggaggc
     4261 gtagcggtgg accgagcgca gcacgccgcc cgcccagggc tggccgtgcg tgagcgcctc
     4321 gacggagctc tgcgcgcccg cgacgctggt ctcgaagaac gcgtagaggt agagcccgct
     4381 cgcgacgatg agccacatca ggtagtacga gatcggcccg atgtagtaga gcgggttcag
     4441 cttctcgccg aaggcgaggt tgaacagccc ctcgaccttc gtgaagcccc attgcagcgc
     4501 ggcctgaatg cgtcccagca ccttgcgatg ctagggcgag ggctccagcg tggatttgat
     4561 gtgagtcaac cgcctgcggc gccattcccg cagcacgaag ccgatgccgc ccagcatgac
     4621 actggtgccg acgaggattt cgatcagcac cacgtagttc aggcggtagc ggcccgccgc
     4681 ggggtcgtac accgtgcaga ggagcttgaa tttctcccat gcggtctcga ggctcggctg
     4741 cgccaccggc gcgttccgcg cgagctcgag cagcgggccg acgaagcggg gcgagtcgaa
     4801 cgagtcgccg tagagctgcc ggtagatgcg gccttcgccg tccacgatgc tcacctgcag
     4861 caggtggtcg aagcccgccg ccgtcgcctc gtagcggaag ccgaactcgc gcgtgagcgc
     4921 cgccaccgtc cccgcttccg gcgtgaggaa cagccagtgg ggcgagccga cgccggcctt
     4981 tttctggaac gcgcgcatcg agcccgggtc gtcgaagggg aggttgaagc cgatcgtcgc
     5041 cacgcggaaa gtgcccgggc cgagcgtgcg ctcggcctcg cgcaccgcgc tcgcgaggaa
     5101 ctgcgttgtc gccgggcaga cctggaaaca gcccgtgtag acgaagtgca ccacgagcgg
     5161 cttgccgcgc agctcggcca ggctcactcg ccggttatcg gaatcgatca ggcggtagtc
     5221 gccgatttgg cggccgagcg cggcctggct gtcctgcagc gccgctttct cgtcgaaagc
     5281 gtgggcgttt cccagaaaga gaaggaagct aagagagaag cgccacatcg gccatcgcgc
     5341 cgaagagcac cgcgcagagc tgcaccagcg aggcgaggaa cgcggcgcgg gcggtggcgc
     5401 ggccgggcgc gcgcatgagc gcgagcgact ttgcgaggaa ataggcgccg ccggcgagcg
     5461 ccgccccgag gtacagcgcg cccaggccga agaagaacgg caccaccgag gcggcgacga
     5521 gcagtcctgc cgccgcgtgc accgcgcgcg cggcgcgcgc atcgccgacc accaccggca
     5581 gcatcggcac gccggccgcc gcgtaatcct cgcggcaggc gatggcgagc gaccagaagt
     5641 gcggcggcgt ccagaggaag agcaccgcag ccagcgcgag cgcttcgggc ccgggcgcgg
     5701 gcgagaccgc ggccgcgccc gcgagcaccg cgaagctgcc ggcgagcccg ccgatcacga
     5761 tgttgcacca cgtgcgccgc ttgagccata cggtgtagac cacgccgtag gtgaacgcgc
     5821 cgaggaacac gtacagcccg gcgaggaaat tcgccgcgag cgccgccgcg agcaccgagc
     5881 ccgcggtcat cgcgccgatc acccagagcc agaacggccc gtggcggagc gcgccggtga
     5941 cgaagggccg gtttcgcgtg cgcgccatct tcacgtcgat gtcgcgctcg acgtactggt
     6001 tgaaggcgcc cgccgcggcc gaggccacga gcaccgcgag gaccaggacg agcccctgga
     6061 agaatcccgc ggaaccctgc tcgaccgcaa ggcctgccgc ggcggcgagc gcgatcgcga
     6121 cgccgatccg cagcttgaag agattgacga aggctctcac ctgcatcccc tacttcagcg
     6181 gccacaccgt cgagaggtac ttccagttga cgaagtagta gaggacgaac gaggtcagga
     6241 acaccagcgc gaggacgaag gtgcccggcg cgaccatccc cgccgagccg tggcccgtga
     6301 ggaccgcgcc gctgcccgcc gcgggcgcgg acgccatgcc gtcgcgcgcg cgtacgtgca
     6361 ggaagcccgg ccggtctagg cgcttgccga ggaacaccga ccatacggtg atgagcaggt
     6421 acatgccgcc gccgacgacc gcgatcaggc cggagaagcc ggcgagcgcc atcatcagcc
     6481 acgccgcgcc cgggaactcg tacgggaagg cgttgccggc gaatcccatg tcccagtgcc
     6541 ggcgaggcac gccgagcgtg ccggcgccca tgaggaagag ggcgacgccg ctcatcgcca
     6601 gcccgaagac gtacggctgg tatttcgcca gcgtcggaaa ggcgagctcg cggcgaaaca
     6661 gcaccgggat gaggaagtag gtgaggccca tgaaggcgag cgtggtgccg atcaccaccg
     6721 tggcgtggaa gtgccccggc acgtagatcg tgttgtggat gatgaggttg agctgctcgg
     6781 tgcccatcat cacgcccgag atgccgccga ggaagccgaa gcccacgagc gagatgaaca
     6841 tcgaggagaa caccgggttg ccccacggcg ccttgcgcag ccactcgaac agcccctgcg
     6901 tgtagccctt cttcctctgc gccacttcga tcgcgcccgg cacggtgagg ccgtgcacca
     6961 tggaggcgag caccgcgagg tacatcgcgt agctggtgtt gaagatcttc cagttggaag
     7021 tgagccccgg gtcggtgagc aggtggtgcg cgctcgcgag ctggaggaag aagatgtaga
     7081 gcaggaaggc ggtgcggctc accttctcgg acatcggctt cgcgccgaac acgatcgcgg
     7141 cgatggcgta ccacaccgag acgtgggcgg cgacgttgac ctgctgcgac gagtgcccga
     7201 aggcccacca gatggtcttg tacatgagcg aatcgatgta ctcgaccagg cccaccgacc
     7261 agaggaaggt cgggatcagg atgatcgcgc c
//
LOCUS       DASRIE010000027         7742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1337513, whole genome shotgun sequence.
ACCESSION   DASRIE010000027 DASRIE010000000
VERSION     DASRIE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337513"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFB53_01320"
     CDS             <1..1022
                     /locus_tag="VFB53_01320"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HZN85399.1"
                     /translation="ERVNTAAAVPQLAEAAADVRALGAALLAQGVAAEQLTQMTSALN
                     DSITQRVIELSEPRHGLEGAWCWLALGSEGRLEQTLATDQDNALIFTVAGGGPEAARA
                     RFLAFAAEVNLALDACGFPLCKGEIMARNPKWCLTLEEWRALFGGWMRVPQPGALLNA
                     CIFFDFRALAGEARLAGELRAQVLRAAAAPEHAAFRYCMAQNALLVRPPLGLLREFAT
                     EEGGEFPGTLNLKTYGARPLVDGARLLALAGALPATSTAARLRAAAAAGLLPQAEADA
                     LLDAFHFIQSLRLRRQYVESGVPPGAENRIDPEQLNAIDRRILKAAFRQANVLQERLK
                     QDYAL"
     gene            1019..1648
                     /locus_tag="VFB53_01325"
     CDS             1019..1648
                     /locus_tag="VFB53_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZN85400.1"
                     /translation="MILRLRRWLGLERAGDELPLRAPRWVVVDCESGGLDPRRDPLLS
                     VGAVAVREGRIQHAESFGAVLRQARASDAANILIHGIGAEAQMLGRDPGAVLRDFVHF
                     AGEGPLVAFHAAFDRALLARAIPGWQPRWLDLAQLAPALFPARAAACRSLDEWLAAFS
                     IGHPARHDALADAYATAQLLLVLLAEAERQRLGRVRDLFATERGRRWLG"
     gene            1678..2673
                     /locus_tag="VFB53_01330"
     CDS             1678..2673
                     /locus_tag="VFB53_01330"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HZN85401.1"
                     /translation="MEKFKAFLITQQDKGVRCAWTDMALEDLDPGELVVRVAYSDVNY
                     KDALAATGKGKILLRPACVGGIDLAGTVVSSSAPQFAKGDAVLAVGHLLGVKHHGGYA
                     EYARIPAAWAVKLPKGMTLWEAMAFGTAGYTAGLAIVKMELNGLKPDNGPVVVDGATG
                     GVGSIALAALAKLGYSVTAMTGKEGESGWLKKLGAKEVLLRQSIDFSKIRPLDKATWA
                     GAVDNLGGETLAWMASTMKFNGTIASIGLAASPGLNTTVMPFILRGASLLGINSTDAP
                     SPELEREVWRRLASDMRPPMLKDMARTVPFADLEQVFGGFIDARVTRRVVVDMSA"
     gene            2670..4562
                     /locus_tag="VFB53_01335"
     CDS             2670..4562
                     /locus_tag="VFB53_01335"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HZN85402.1"
                     /translation="MTGYREFHRRSIGERDAFWAGEAKLIDWHKPFGRVLDYSRPPFA
                     KWFVGGETNLCHNAVDRHLAQRGGQKALQWISTEVDAARSYTYRELHAEVNRTAAMLL
                     ALGAKWGDRVIIYMPMVPEACFAILACARIGAIHSVVFGGFAAASLAARIDDARPALM
                     ITADGGSRMGKPVLYKALVDESLKLASHPPRKVLIFRRGLDAGMALTPGRDVDWAELA
                     ARHQGASVPCAWLESSEPSYILYTSGTTGKPKGVQRDVGGYAVALASSMARIFCGGAG
                     ETMFTTSDIGWVVGHSYIIYGPLLAGMTTILYEGVPVRPDAGVWWKIVQDHNVAVMFS
                     APTAIRVLKKHDVAYLKKYDVSSLKHLFLAGEPLDEPTHRWIAESLGRPVIDHYWQTE
                     TGWPLLSAHPGVEQTPIKMGSPSFPVYGYDVRLLHEGTGRPVADGEKGVVAIAPPLPP
                     GCMSTVWGDDARFVDTYFRDFKDQLVYTTFDWGIRDKDGYYFILGRTDDVINVAGHRL
                     GTREIEEAVNMHPGIAECAVVGVADPLKGQLPMAFAVVKDPAGIASAAGRMKLEGEIM
                     QTVDKQLGAIGRPGRVFFVTLLPKTRSGKVLRRSIQALAEKRDPGDLTTIEDPGALEQ
                     IRQALQ"
     gene            4559..4960
                     /locus_tag="VFB53_01340"
     CDS             4559..4960
                     /locus_tag="VFB53_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN85403.1"
                     /translation="MKPRPALTLADCEKISAAAFAEARRNSWIVAVAILDDGGHLLHF
                     ARMDGASPANARIAIAKGRTAAESRRNTGTWQERIKDRPELMQMPGVLPVQGGVPIFA
                     DGSCVGAVGVSGVQSHEDEKIAQAGIDALLK"
     gene            complement(5007..5465)
                     /locus_tag="VFB53_01345"
     CDS             complement(5007..5465)
                     /locus_tag="VFB53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN85404.1"
                     /translation="MRAAAFLAGLALAAGSATAHAQQRPDYGPEVTIAAAKKIAAATI
                     AECAANKWNVAVAVVDNHGFLVYFERMANTQTASVEIAIGKARSAATYRRTTRAFMEV
                     INKAPATATLPNIMASPGGVPIFAEGKVIGAVGVSGVTGDQDEQCAKAGL"
     gene            complement(5533..6738)
                     /locus_tag="VFB53_01350"
     CDS             complement(5533..6738)
                     /locus_tag="VFB53_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85405.1"
                     /translation="MTSLQARCCIAGGGPAGMMLGFLLARAGVDTIVLEKHADFLRDF
                     RGDTVHPSTLEVMHELGLLEEFLRRPHQKLARIEGQVGGRPIVMADFSRLPVQCRFIA
                     FMPQWDFLDFLAQHGRRLPGFRLMMQAEATGLVEDAGRVAGVRVKTVEGPVEIRAALT
                     VAADGRHSLLRERAGLEVQDFGAPMDVLWMRISKRPDDPPNTLGRINAGRVFVTLDRG
                     DYWQCAYVIPKGGNDALRRGGIESFRENVAAVAPFLRERVGELRGWDDVKLLTVQVNR
                     LRRWHRPGFLCIGDAAHAMSPVGGVGINLAIQDAVAAANALADPLSRGVVGEADLAAV
                     QRRRDFPTRMTQRMQLFLQNRVIGRVLASGATPRIALPIRLLQWFPVLQRIPARLIGM
                     GFRPEHLRR"
     gene            complement(6735..>7742)
                     /locus_tag="VFB53_01355"
     CDS             complement(6735..>7742)
                     /locus_tag="VFB53_01355"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN85406.1"
                     /translation="FNRNKKSLALDLKKPAGREVALKLIDSADAMTENFRPGALDKMG
                     LGYAALSQRNPRLVYCTMKGFLSGPYEHRPALDEVVQMMGGLAYMTGLPDRPLRVGSS
                     VNDIMGGMFAAIGILAALREREKTGKGQLVRSALYENTVFLVAQHMAQLAITGEEPPP
                     MAVKKPAWGIYDIFPTQGGDRLFVGVVTDTQWEVFCREFGAAELAADARLKTNGERVK
                     ARGWLLPKLEEIFRRHGKQELSAKLEAIGLPFAPITRPRDLFDDPHLNAGGMIETRDP
                     RGKAFKVPGLPLELGSKRLPLRRQPPAVSADARELLAGLGYASARIDALFADGAVAAP
                     Q"
BASE COUNT         1142 a         2663 c         2783 g         1154 t
ORIGIN      
        1 tcgaacgcgt gaacaccgcg gcggcggtgc cgcagctggc cgaggccgcc gccgacgtcc
       61 gcgccctggg cgccgcgctg ctggcgcagg gcgtggcggc cgagcagctg acgcagatga
      121 ccagcgccct caacgacagc atcacgcagc gggtcatcga attgtcggag cccaggcacg
      181 gcctggaagg cgcctggtgc tggttggcgt tgggcagcga agggcgcctc gagcagacgc
      241 tggccaccga ccaggacaac gccctgatct tcaccgtggc gggcggcggc ccggaggcgg
      301 cgcgcgcgcg cttcctggcg ttcgccgccg aagtgaacct cgcgctcgat gcctgcggtt
      361 tcccgctgtg caagggggag atcatggcgc gcaacccgaa gtggtgcctt acgctggagg
      421 aatggcgcgc cttgttcggc ggctggatgc gcgtcccgca gcccggggcc ctgctcaacg
      481 cctgcatctt cttcgacttt cgcgcgctgg ccggggaggc gcgcctggcg ggggagctga
      541 gagcgcaggt gctgcgcgcc gcggccgcgc cggagcacgc cgcgttccgc tactgcatgg
      601 cgcagaatgc gctgctcgtg cggccgcctc tcggcctgct gcgcgaattc gcgaccgagg
      661 aaggcggcga gttccccggc accctgaacc tcaagaccta cggcgcgcgc ccgctggtgg
      721 acggcgcgcg cctgctggcg ctcgccggcg ccctgccggc gacttccaca gcggcccggc
      781 tgcgcgccgc agctgccgcc ggcctcctgc cgcaggcgga ggccgatgcg ctgctggatg
      841 cctttcactt tatccagtcg ctgcgcctgc gccgccaata cgttgaatcc ggcgtgccgc
      901 ccggcgcgga gaaccgcatc gacccggaac agctcaacgc catcgaccgg cgcatcctga
      961 aagcggcatt ccgccaggcg aacgtgctgc aggaacggct gaagcaggac tacgcccttt
     1021 gatcctgcgc ctgcggcgct ggctggggct ggagcgcgcc ggggacgagc tgccgctgcg
     1081 cgcgccgcgc tgggtggtgg tggactgcga atcgggcggc ctcgacccgc gtcgcgaccc
     1141 cctcctgtcc gtcggcgccg tcgcggtgcg ggagggacgc atccagcatg ccgagagttt
     1201 cggcgccgtg ctgcgccagg cccgggcgag cgacgccgcg aacatcctga tccacggcat
     1261 cggcgccgag gcgcagatgc tgggacgcga cccgggcgcg gttctgcgcg atttcgtgca
     1321 cttcgccggc gaagggcccc tggtggcctt ccatgccgcg ttcgatcgcg cgctcctggc
     1381 gcgcgccatc ccggggtggc agccgcgttg gctggacctg gcgcagctcg cgccggcgct
     1441 gttcccggcg cgcgcggcag cgtgcaggtc gctggacgaa tggctcgcgg ccttctccat
     1501 cgggcatccg gcgcggcacg acgcgctggc cgacgcttat gccaccgcgc agctgctgct
     1561 ggtgctgctg gccgaagccg agcgccagcg gctggggcgg gtgcgggacc tgtttgccac
     1621 cgagcgcggc cggcgctggc ttggctagaa tgacctcccc ccgacccgga aatccgcatg
     1681 gaaaaattca aggccttcct catcacccag caggacaagg gagtccgctg cgcgtggacc
     1741 gacatggcgc tggaggacct cgacccgggc gagctggtgg tgcgcgtcgc ctactcggac
     1801 gtgaactaca aggacgcgct ggccgccacc ggcaagggga agatcctgct gcggcctgcg
     1861 tgcgtcggcg gcatcgacct tgccggcacc gtcgtttcct ccagcgcgcc gcagttcgcc
     1921 aagggcgacg cggtgctggc ggtgggccac ctgctcggcg tgaagcacca cggcggctat
     1981 gccgaatacg cgcgcatccc ggccgcttgg gcggtgaagc tgcccaaggg catgacgctg
     2041 tgggaggcga tggcgttcgg caccgccggc tacacggcgg ggctcgcgat cgtgaagatg
     2101 gagctgaacg gcctgaagcc cgataacgga ccggtggtcg tcgacggcgc gaccggcggc
     2161 gtgggctcga tcgcgctcgc ggcgctggcg aagctcggct actcggtgac ggcgatgacc
     2221 ggcaaggagg gcgagtccgg ctggctgaag aagctcggcg cgaaggaagt cctgctgcgc
     2281 cagagcatcg atttcagcaa gatccggccg ctggacaagg cgacctgggc gggcgcggtg
     2341 gacaacctcg gcggcgagac cctggcctgg atggcgagca ccatgaagtt caacggcacc
     2401 atcgcctcga tcggcctggc ggcaagcccc ggcctcaaca ccaccgtgat gcccttcatc
     2461 ctgcgcggcg cgagcctgct cggcatcaat tccaccgatg cgccgtcgcc cgagctcgag
     2521 cgcgaagtct ggaggcggct cgcctcggac atgcgcccgc cgatgctcaa ggacatggcg
     2581 cgcacggtgc cgttcgcgga cctggagcag gtgttcggcg gcttcatcga cgccagggtg
     2641 acgcgacgcg tcgtcgtgga catgagcgca tgacgggcta ccgggagttc caccggcgct
     2701 cgatcgggga gcgcgacgcc ttctgggccg gggaagcgaa actcatcgac tggcacaagc
     2761 ccttcggccg ggtgctggat tattcgcgcc cgccgttcgc caagtggttc gtcggcggcg
     2821 agaccaacct gtgccacaac gctgtcgacc ggcacctcgc gcagcgcggc gggcagaagg
     2881 cgctgcagtg gatctccacg gaagtggacg cggcgcgcag ctacacctac cgggaactgc
     2941 acgcggaggt gaatcgcacg gcggccatgc tgctggcgct gggagcgaag tggggcgatc
     3001 gcgtgatcat ctacatgccg atggtgcccg aggcctgctt cgccatcctc gcctgcgcgc
     3061 gcatcggcgc catccactcg gtggtgttcg gcggcttcgc cgcggccagc ttggcggcgc
     3121 gcatcgacga cgccaggccc gcgctgatga tcactgccga cggcggcagc cgcatgggca
     3181 agcctgtgct ttacaaggcg ctggtggacg agtcgctcaa gctcgccagc cacccgccgc
     3241 gcaaggtcct catcttccgg cgcggcctgg acgcgggcat ggcgctgacg cccgggcgcg
     3301 acgtcgactg ggcggagctc gccgcgcggc accagggcgc aagcgtgcct tgcgcgtggc
     3361 tcgagtcgag cgagccgtcc tacatcctct acacctccgg caccaccggc aagcccaagg
     3421 gcgtgcagcg cgacgtcggc ggctacgcgg tggcgctcgc ctcctcgatg gcgcgcatct
     3481 tctgcggcgg cgcgggcgag accatgttca ccacctcgga tatcggctgg gtggtgggcc
     3541 attcctacat catctacggg ccgctgctcg ccggcatgac caccatcctg tacgagggcg
     3601 tgccggtgcg ccccgacgcg ggcgtctggt ggaagatcgt gcaggaccac aacgtggcgg
     3661 tgatgttctc cgcgcccact gcgatccgcg tgctgaagaa gcacgacgtc gcgtacctga
     3721 agaagtacga cgtcagctcg ctcaagcacc tgttcctcgc cggcgagccg ctggacgagc
     3781 ccacgcaccg ctggatcgcg gagtctctcg gccgcccggt gatcgaccac tactggcaga
     3841 ccgagaccgg ctggccgctg ctgtcggcgc acccgggggt ggagcagacg ccgatcaaga
     3901 tgggctcgcc gagcttcccg gtctacggct acgatgtacg gctgctgcac gagggcaccg
     3961 gccggccggt ggcggacggg gagaagggcg tggtggccat cgcgccgccg ctgccgccgg
     4021 gctgcatgag cacggtgtgg ggcgacgacg cgcgcttcgt cgacacctac ttccgggact
     4081 tcaaggacca gctcgtctac accaccttcg actggggcat ccgggacaag gacgggtact
     4141 acttcatcct cggcaggacc gatgacgtga tcaacgtcgc cggccaccgc ctggggacgc
     4201 gcgaaatcga ggaagcggtg aacatgcatc cgggcatcgc ggaatgcgcg gtggtcggcg
     4261 tggccgaccc gttgaagggc cagctgccca tggcattcgc ggtggtcaag gatccggcgg
     4321 gcatcgccag cgcggcaggc cgcatgaagc tggagggcga aatcatgcag acggtggaca
     4381 agcagctggg cgccatcggc cgcccggggc gggtgttctt cgtcacgctg ctgcccaaga
     4441 cgcgctcggg caaggtgctg cggcgctcga tccaggcgct cgccgagaag cgggacccgg
     4501 gagacctgac gaccatcgag gatccgggcg cgctcgagca gatccggcag gcgctgcaat
     4561 gaaaccgcgg ccggcgctga ccctcgccga ctgcgagaag atctccgcag cggcgttcgc
     4621 cgaggcgagg cgcaacagct ggatcgtcgc cgtcgccatc ctcgacgacg gcgggcacct
     4681 gctgcatttc gcgcgcatgg acggggccag ccccgccaat gcgcgcatcg ccatcgccaa
     4741 gggacgcacc gccgcggagt cgcggcgcaa caccggcact tggcaggagc gcatcaagga
     4801 ccgcccggaa ttgatgcaga tgccgggcgt gctgccggtg cagggcggcg tgccgatctt
     4861 cgccgacggc agctgcgtgg gcgcggtcgg cgtgtcgggc gtgcagtccc acgaggacga
     4921 gaaaatcgcc caggcgggga tcgacgcgct gctgaaatga aaacggggcc gcgcggcccc
     4981 gtccggaaat caatccgcgg aaatgcctac agccccgcct tggcgcactg ctcgtcctgg
     5041 tcgccggtga cgccgctcac gccgaccgcg ccgatgacct tgccttcggc aaagatcggc
     5101 acgccgccgg ggctggccat gatgttcggc agggtggcgg tggccggggc cttgttgatc
     5161 acctccatga acgcgcgcgt ggtgcgccgg taggtggccg ccgagcgcgc cttgccgatg
     5221 gcgatttcca cgctggcggt ctgggtgttc gccatgcgct cgaagtagac gaggaagcca
     5281 tggttgtcga ccaccgcgac ggcgacgttc cacttgttcg cggcgcactc ggcgatggtg
     5341 gccgcggcga tcttcttcgc ggcggcgatg gtgacctcgg ggccgtagtc gggccgctgc
     5401 tgcgcgtggg cagtggctga ccctgcggcg agcgcgagtc ccgccaggaa tgctgctgct
     5461 ctcattgtcc tctctccttg ggttggtggt gatgcacccc gcaaacaggg tggccggtac
     5521 tctattccag ttctagcgcc gcaggtgctc gggacgaaat cccatgccga tgagccgcgc
     5581 cgggatgcgc tgcagcacgg gaaaccactg cagcaaccgg atcggcagcg cgatgcgcgg
     5641 cgtggcgccg ctcgccagga cgcggccgat gaccctattc tgcaggaaca gctgcatgcg
     5701 ctgggtcatg cgggtcggga aatccctgcg gcgctgcacc gccgccaggt cggcctcgcc
     5761 caccacgccg cgcgacagcg ggtcggcgag tgcattggcc gccgccaccg cgtcctggat
     5821 cgcgaggttg atgccgacgc cgccgaccgg cgacatggcg tgcgccgcgt cgccgatgca
     5881 caggaacccc gggcggtgcc agcggcgcag ccggttcacc tgcacggtaa ggagcttcac
     5941 gtcgtcccag ccgcgcagct cgcccacgcg ctcgcgcagg aaaggcgcca cggcggcgac
     6001 gttctcgcgg aaggactcga tgccgccgcg ccgcagggcg tcgttgccgc ccttcgggat
     6061 cacgtaggcg cattgccagt agtcgccgcg gtccagggtc acgaacacgc gcccggcgtt
     6121 gatgcggccg agggtgttcg gcgggtcgtc ggggcgcttg gagatgcgca tccacagcac
     6181 atccatcggc gcgccgaagt cctgcacctc gagcccggcc cgctcgcgca gcagcgaatg
     6241 gcggccgtcg gctgccaccg tgagcgctgc ccggatctcg acgggtcctt cgacggtttt
     6301 gacccgtacg ccggcgacgc gccccgcgtc ctcgaccagg ccggtcgcct cggcctgcat
     6361 catcaggcgg aatcccggca gcctgcgccc gtgctgcgcg aggaaatcga ggaaatccca
     6421 ttgcggcatg aaggcgatga acctgcactg caccggcagg cgggagaagt cggccatcac
     6481 gatcgggcgg ccgccgacct ggccctcgat gcgcgcgagc ttctggtgcg gccgccgcag
     6541 gaattcctcc agcaagccca gctcgtgcat cacttcgagc gtggaagggt gcacggtgtc
     6601 gccgcggaag tcgcgcagga agtcggcgtg cttctccagc acgatggtgt cgacgccggc
     6661 gcgcgccagg aggaatccca gcatcatgcc tgcggggccg ccgccggcga tgcagcagcg
     6721 cgcctgcagg ctggtcactg cggtgcggcg accgcgccgt cggcgaacag cgcgtcgatg
     6781 cgcgcgctcg cgtagcccag cccggccagc agctcgcgcg cgtcggccga gaccgccggc
     6841 ggctggcggc gcaaggggag ccgcttgctg ccgagctcca gcggcagccc gggcaccttg
     6901 aacgccttgc cgcgggggtc cctggtctcg atcatgccgc cggcgttgag gtgcggatcg
     6961 tcgaacaggt cgcgcgggcg cgtgatcggg gcgaagggca ggccgatggc ctccagtttc
     7021 gccgacagtt cctgtttgcc gtggcgcctg aagatctctt ccagcttcgg cagcagccag
     7081 ccgcgcgcct tgacgcgctc gccgttggtc ttcaggcggg cgtcggccgc cagctcggcg
     7141 gcgccgaact cgcggcagaa cacctcccac tgcgtgtcgg tgaccacccc gacgaacagg
     7201 cggtcgccgc cctgggtcgg aaagatgtcg tagatgcccc aggcaggctt cttcaccgcc
     7261 atcggcggcg gctcctcgcc ggtaatggcg agctgcgcca tgtgctgcgc caccaggaac
     7321 accgtgtttt cgtagagcgc gctgcgcacc agctgtccct tgccggtttt ttcgcgctcg
     7381 cgcagcgcag caaggatgcc gatggcagcg aacatgccgc ccatgatgtc gttcaccgag
     7441 gagccgacgc gcaacgggcg gtcgggcagg ccggtcatgt aggcaaggcc gcccatcatt
     7501 tgcaccactt cgtcgagcgc cgggcggtgc tcgtagggcc ccgacaggaa tcccttcatc
     7561 gtgcaataga ccaggcgcgg gttgcgctgc gacagcgctg cgtagcccag ccccatcttg
     7621 tccagggcgc cggggcggaa gttctccgtc atcgcgtcgg cgctgtcgat cagcttgagg
     7681 gcgacttccc ggcccgcggg cttcttcagg tcgagggcga ggctcttctt gttgcggttg
     7741 aa
//
LOCUS       DASRIE010000028         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3419975, whole genome shotgun sequence.
ACCESSION   DASRIE010000028 DASRIE010000000
VERSION     DASRIE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419975"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFB53_01360"
     CDS             <1..430
                     /locus_tag="VFB53_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85407.1"
                     /translation="WDEVIDINLKAVFRLSRAVMRGMMKARWGRIINITSVVGAAGNA
                     GQSNYAAAKAGVVGMTKSLARELGGRGITVNSVAPGFIDTDMTRALTEAQRTALLGQI
                     PLGRLGAPEDVAAAVAYLASPAAAYVTGCVLHVNGGMYMA"
     gene            496..735
                     /gene="acpP"
                     /locus_tag="VFB53_01365"
     CDS             496..735
                     /gene="acpP"
                     /locus_tag="VFB53_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN85408.1"
                     /translation="MENVEQRVRKIVAEQLGVNEGEIKNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDYVKSHAKS"
     gene            749..1981
                     /gene="fabF"
                     /locus_tag="VFB53_01370"
     CDS             749..1981
                     /gene="fabF"
                     /locus_tag="VFB53_01370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZN85409.1"
                     /translation="MSRRRVAVTGLGIVCPVGNTVEEAWRNLVAGRPGIGPITRFDAA
                     PFSCRIAGEVKGFDVSAYLPAKDARHMDAFIHYGLAAAIQAWKDCGLQVTEQNAERIG
                     VNIGSGIGGLPRIEDTHGELTQRGPRRISPFFVPASIINMISGHMSILHGMKGPNLAV
                     VTACTTGLHAIGLSARTIEYGDADVMVAGGAEATISPLGVGGFAAARALSTRNDDPPT
                     ASRPWDRDRDGFVLGEGAGALVLEEYEHARARGAKIYCELTGFGMSADAFHMTAPDQD
                     GPRRCMQAALANARIAPDRVQYLNAHGTSTPLGDKNETEAIKRTFGGHAHRLVVNSTK
                     SMTGHLLGGAGGIESVFTALAVQRQVSPPTINIFNQDPECDLDYCANTARDMKIDCAV
                     KNNFGFGGTNGTLVFARV"
     gene            1978..2421
                     /locus_tag="VFB53_01375"
     CDS             1978..2421
                     /locus_tag="VFB53_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HZN85410.1"
                     /translation="MSRGPLLRLELRPSRALAVVLGLVHAAGAACVLVVAGAGTAGPA
                     LAALLLGLGAATIRDRALLRGGGAVRAIELGGEGAAALELADGRRLAGRVHGRRHVSR
                     LWVTLPLRGSPHRSVLVAADMLAPAAFRQLRLWALWGRVARGGRA"
     gene            2515..3114
                     /gene="rpoE"
                     /locus_tag="VFB53_01380"
     CDS             2515..3114
                     /gene="rpoE"
                     /locus_tag="VFB53_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZN85411.1"
                     /translation="MSERDIDRQLVERAQRGDKHAFELLVEKYQRKLGRLLARFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLMALGRRAPTSTEVEA
                     EDAEGYEEGEQLRDINTPESMLLSKEIAQTVNATIEQLPEELRTAIQLREIEGMSYED
                     IARIMDCPIGTVRSRIFRAREAIAERLRPLLDSPKDQRW"
     gene            3119..3655
                     /locus_tag="VFB53_01385"
     CDS             3119..3655
                     /locus_tag="VFB53_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HZN85412.1"
                     /translation="MKSKISALMDGELDAGELHEPLSALHQDPGARDAWRTYHLISDA
                     LQGRALVRTDCLSRVAARLAQEPVPVAAQPAAVRPPERARWFVPSALAASLAAVALVG
                     WVAFAPPAGNAPAPLARAPAPVVAQAERAQEPARVPLTSASRDYLLAHQAFSPRNGLL
                     GMAPYVRSVSSDAGAKKP"
     gene            3652..4611
                     /locus_tag="VFB53_01390"
     CDS             3652..4611
                     /locus_tag="VFB53_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HZN85413.1"
                     /translation="MSRALGALLLCAASGLAQAQAAPEALEWLRKIHQATQKLSYTGV
                     FVYQQGGRTETSRITRRADAGGGAEKLEVLDGTPREILRTQDEIRCYLPESQVVKVDQ
                     RRDQRGFPSLLPEQVNALAEHYAIDTGGVQRIAGYDCQVLQLTPRDNLRYGYQLWADL
                     RTGMLLKARAFDAKGETVEQFTFTQLDIGSVPRGRLRPSRAARGWRVEQAGVAPADLS
                     RSGWSMSADLPGFRKIVEIRRMLRDAQPVGQVVYSDGLAAVSVFIEPLAGRSEAARLG
                     LATMGALNVYTREVADHLVTVVGEAPAGSVQRIGNTVEYHRPQ"
     gene            4650..6071
                     /locus_tag="VFB53_01395"
     CDS             4650..6071
                     /locus_tag="VFB53_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZN85414.1"
                     /translation="MHLIRLGAALALAACTTLAAAQGRELPDFTRLVDEQGAAVVNIS
                     TTQAPRRGANVPQLPNLEDEEMLEFFRRFIPRQPGPGARPESRSLGSGFLISADGYIL
                     TNAHVIDAADEINVKLTDKREYKAKVIGADKRTDVALIKIDAANLPAVRLGDPAKLKV
                     GEWVVAIGSPFGFENTVTAGIVSAKGRSLPQENFVPFIQTDVAINPGNSGGPLFNMKG
                     EVVGVNSQIYSRTGGFMGLSFAIPIDVALDIQKQLRDKGRVSRGRIGVVIQEVTKDLA
                     VSFGLDRPRGALVNAVEKGSPADRGGIEATDIITRFDGKLVESSSDLPRLVGATRPGT
                     SVPVEVWRKAAARTLSLTVGELQEDKVAARSAPRERKPAELAANRLGIAVSELTAEQK
                     RELKLGHGLMVTDVRPDARAEVRRGDILLKMVHNGQHTDLKSNEQLNRLLAGMDKNAV
                     ITLQVRRGESTAFVTVTGLADKG"
     gene            6075..6299
                     /locus_tag="VFB53_01400"
     CDS             6075..6299
                     /locus_tag="VFB53_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZN85415.1"
                     /translation="MLRLYGRKYCHLCDDMARALRALGVAFAEFDVDADPELEARYGE
                     LVPLLADAAGVEICHYHLDEAALRARVAVR"
     gene            6340..>7298
                     /locus_tag="VFB53_01405"
     CDS             6340..>7298
                     /locus_tag="VFB53_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZN85416.1"
                     /translation="MTPQSRIRNFSIIAHIDHGKSTLADRIIQLCGGLAEREMAEQVL
                     DSMDLERERGITIKAQTAALQYQARDGERYLLNLIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQTVANCYTAIEQGVEVVPVLNKIDLPSADPEKAIQEIEDVIGIRAQG
                     ALRASAKTGDGVPEILEAVIARIPPPKGDPDARLQALVIDSWFDNYVGVVMLVRMMQG
                     RLRPKDKVLLMSTGAAHLCEQVGVFTPKSQPREELVAGEVGFVVAGVKELREAKIGDT
                     ITRGDRPAAAPLPGFKEIKPQVFAGLYPVESNQYEALRDALEKL"
BASE COUNT         1168 a         2472 c         2661 g          997 t
ORIGIN      
        1 atgggacgag gtgatcgaca tcaacctgaa agccgtgttc cggctgtcgc gcgcggtgat
       61 gcgcggcatg atgaaggcgc gctggggccg catcatcaac atcacgtcgg tggtcggcgc
      121 ggccggcaac gccggccagt ccaactatgc cgccgccaag gcgggggtgg tcggcatgac
      181 caagtcgctg gcgcgcgagc tgggcggccg cggcatcacc gtcaacagcg tggcgccggg
      241 gttcatcgat accgacatga cgcgcgcgct gaccgaggcg cagcgcaccg cgctactcgg
      301 ccagattccg ctgggacggc tgggagcgcc ggaggatgtc gcggcagcag tggcctatct
      361 ggcgtcgccg gcggccgcct acgtcaccgg atgcgtgcta cacgtgaacg gcggcatgta
      421 catggcctga ggccgcgcgc gccgatttgg taaaatccgg gccgccgctt ggccccaaca
      481 gggaaggagc agaacatgga gaacgtcgaa caacgcgtca ggaagatcgt cgccgagcaa
      541 ctcggggtga acgagggcga gatcaagaac gagtcctcct tcgtcgacga cctgggggcc
      601 gactcgctcg acacggtgga gctggtgatg gcgctggagg aggaattcga gaccgagatt
      661 cccgacgagg aagccgagaa gatcaccacc gtccagcagg cgatcgacta cgtcaagtcg
      721 cacgcgaagt cgtagctcca agcgctaagt gtcgcgccgc agagtcgccg tcaccgggct
      781 gggcatcgtc tgcccggtcg gcaataccgt ggaggaagcg tggcgcaacc tggtcgcggg
      841 ccggcccggg atcggcccga tcacgcgctt tgacgcggcg ccgttcagct gccgcatcgc
      901 cggcgaggtg aagggcttcg acgtctccgc ctacctgccg gcgaaggacg cgcgccacat
      961 ggacgccttc atccactacg gactggcggc agccatccag gcgtggaagg actgcggcct
     1021 gcaggtcacc gagcaaaacg ccgagcgcat cggcgtgaac atcggctcgg gcatcggcgg
     1081 cctgccgcgc atcgaggaca cgcacggcga actgacgcag cgcggcccgc gccgcatctc
     1141 gccgttcttc gtgccggctt cgatcatcaa catgatttcc ggccacatgt cgatcctgca
     1201 cggcatgaag ggaccgaacc tcgccgtggt gaccgcctgc accaccggat tgcacgcgat
     1261 cggcctgtcc gcgcgcacga tcgagtacgg cgacgccgac gtgatggtgg ccggcggcgc
     1321 cgaggcgacc atctcgcccc tgggcgtcgg cggcttcgcc gcggcgcgcg ccctgtcgac
     1381 gcgcaacgac gacccgccca ccgccagccg cccctgggac cgcgaccgcg acggcttcgt
     1441 cctgggggag ggggccggcg cgctggtgct ggaggaatac gagcacgccc gggcgcgggg
     1501 cgcgaagatc tattgcgagc tgaccggctt cggcatgagc gcggacgctt ttcacatgac
     1561 ggcgccggac caggacgggc cccggcgctg catgcaggcg gcattggcca atgcgcgcat
     1621 cgcgccggac cgcgtgcagt acctgaacgc gcacggcacc tcgacccccc tgggcgacaa
     1681 gaacgagacc gaggccatca agcgcacgtt cggcggacat gcgcaccggc tggtggtgaa
     1741 ctccaccaag tcgatgaccg gccacctgct gggcggcgcc gggggcatcg aatcggtgtt
     1801 caccgccctg gcggtgcagc ggcaggtgtc gccgcccacc atcaacatct tcaaccagga
     1861 cccggagtgc gacctggact actgcgccaa cacggcgcgg gacatgaaga tcgactgcgc
     1921 cgtcaagaac aacttcggct tcggcggcac caacggcact ctggtgttcg cccgcgtttg
     1981 agccgcgggc cgctcctgcg cctcgaatta cgcccttcgc gcgcgctggc cgtcgtcctg
     2041 ggcctggtgc atgccgccgg cgcggcctgc gtgctggtcg tcgcgggagc gggcaccgcc
     2101 ggcccggcgc tggcggcgct gctcctcggg ctgggcgcgg cgacgatccg cgaccgtgcc
     2161 ctgctgcgtg gcggcggcgc ggtgcgcgcc atcgagctgg gcggggaggg cgcggcggcg
     2221 ctggaattgg cggatggcag gcgcctggcc ggccgcgtgc acggccgccg gcacgtgtcc
     2281 cgcctctggg tgacgctgcc cctgcgcggc tcgccgcacc gcagcgtact ggtggcggcg
     2341 gacatgctgg cgcccgccgc tttccgccag ctgcgcctgt gggcgctctg gggcagggtg
     2401 gcgcgcggcg gccgggcatg agccgaacta tttccggggc cgcgtgtctg tggcacaggg
     2461 gtgttgcggt ccggtgcggc gcgagtatgc tgcgcgggtc cggcgggtgg tcgagtgtct
     2521 gagcgggata tcgaccgtca gctggtggag cgggcgcagc ggggcgacaa gcacgcgttc
     2581 gagctgctgg tggagaagta ccagcgcaag ctggggcggc tgctggcgag attcatccgc
     2641 gaccccgcgg aagtggaaga cgtgacgcag gaagcgttca tcaaggcgta ccgggcgctg
     2701 ccggcgtttc gcggcgacag cgcgttctac acgtggctgt accgcatcgg catcaacacc
     2761 gccaagaatt acttgatggc gctggggcgg cgggcgccga cctccaccga ggtggaggcg
     2821 gaagacgccg aagggtacga ggaaggcgag cagttgcgcg acatcaacac gcccgagagc
     2881 atgttgctgt ccaaggagat tgcgcagacc gtgaatgcga ccatcgaaca gctgcccgaa
     2941 gagctgcgca cggcgatcca gctgcgcgag atcgagggca tgagctacga ggacatcgcg
     3001 cgcatcatgg attgcccgat cggcacggtg cgctcgcgca ttttcagggc gcgcgaggcg
     3061 atcgcggagc gcctgcggcc gctgctcgat tcgcccaagg accagaggtg gtagcaggat
     3121 gaaatccaag atctcggcgt tgatggacgg cgaactcgac gccggcgagc tgcacgagcc
     3181 gctctccgcg ctgcaccagg atccgggcgc gcgcgatgcc tggcgcacct accacctgat
     3241 cagcgatgcg ctgcagggcc gcgccctggt gcgcaccgat tgcctgtcgc gcgtcgccgc
     3301 ccggctggcg caggagcccg tgccggtggc ggcgcagccc gcggcggtgc gcccgccgga
     3361 gcgggcgcgc tggttcgtgc cctcggcgct ggcggccagc ctcgccgcgg tggccctggt
     3421 cggctgggtg gcgttcgcgc ccccggcggg caacgcgccg gcgccgctcg ccagggcccc
     3481 ggcgccggtg gtcgcgcagg ccgagcgagc gcaggagccg gcgcgcgtgc cgctcaccag
     3541 cgcgtcgcgc gactacctgc tggcccacca ggccttctcg ccgcgcaacg ggctgctcgg
     3601 catggcgccc tacgtgcgct cggtttccag cgacgcgggg gcgaagaagc cgtgagccgc
     3661 gccctgggcg cgttgctgct gtgcgccgcg tcagggctgg cccaggcgca ggccgcgccc
     3721 gaggcgctgg agtggctgcg caagatccac caggcgacgc agaaactgtc ctacacgggc
     3781 gtgttcgtct accagcaggg cggccgcacc gagacctcgc gcatcacgcg ccgggccgat
     3841 gccggcggcg gcgcggagaa gctggaggtg ctcgacggca cgccgcgcga gatcctgcgc
     3901 acgcaggacg agatccgctg ctacctgccg gagagccagg tggtgaaggt cgatcagcgc
     3961 cgcgaccagc gcggctttcc ttcgctgctg cccgagcagg tgaacgccct cgccgagcac
     4021 tatgcgatcg acacgggcgg ggtgcagcgc atcgccggct atgactgcca ggtgctgcag
     4081 ttgacgccgc gcgacaacct gcgctacggc taccagctgt gggccgatct gcgcaccggc
     4141 atgctgctca aggcgcgggc cttcgacgcc aagggcgaga ccgtggagca attcaccttc
     4201 acgcagctgg acatcggcag cgtgccgcgc gggcggctgc ggccgagccg tgcagcgcgc
     4261 ggctggcgcg tcgagcaggc cggcgtggcg ccggcggacc tgtcgcgctc cggctggtcg
     4321 atgagcgcgg acctgcccgg attccgcaag atcgtcgaaa tccgccgcat gctgcgcgac
     4381 gcgcagccgg tcggccaggt cgtgtattcc gacggcctgg cggccgtgtc ggtgttcatc
     4441 gagccgctcg ccggacgcag cgaagccgcg cgcctgggcc tggccaccat gggcgcgctg
     4501 aacgtctaca cgcgggaagt cgccgaccac ctggtgaccg tggtgggcga ggccccggcc
     4561 ggcagcgtgc agcgcatcgg caatacggtc gagtaccacc gcccgcagta ggcatccacg
     4621 ccgtcccggc acacacgaga ggattctcca tgcacctgat cagactgggc gccgcgctgg
     4681 cgctcgccgc ctgcaccact ctggccgccg cccaggggcg cgagctgccc gatttcacgc
     4741 gcctggtgga cgagcagggc gccgcggtgg tgaacatcag caccacccag gcgccgcggc
     4801 gcggcgccaa cgtgccgcag ctgccgaacc tggaggacga ggagatgctg gagttcttcc
     4861 gccgcttcat cccgcgccag cccgggcccg gcgcgcgccc cgagagccgc tcgctcggct
     4921 cgggcttcct catcagcgcc gacggctaca tcctgaccaa tgcgcacgtc atcgacgccg
     4981 ccgacgagat caacgtcaag ctcaccgaca agcgcgagta caaggcgaag gtgatcgggg
     5041 cggacaagcg caccgacgtg gcgctgatca agatcgacgc cgccaacctg ccggcggtgc
     5101 gcctgggcga cccggccaag ctcaaggtgg gcgagtgggt ggtggcgatc ggctcgccct
     5161 tcggcttcga gaacacggtg accgccggca tcgtcagcgc caagggccgc tcgctgccgc
     5221 aggagaactt cgtgcccttc atccagacgg acgtggccat caacccgggc aattccggcg
     5281 ggccgctgtt caacatgaag ggcgaggtgg tcggggtcaa ctcgcagatc tacagccgca
     5341 ccggcggctt catgggcctg tcgttcgcca tcccgatcga cgtggcgctc gatatccaga
     5401 agcagctgcg cgacaagggc cgcgtgtcgc gcggccgcat cggcgtggtg atccaggagg
     5461 tgaccaagga cctggcggtg tccttcgggc tggaccggcc gcgcggcgcg ctggtgaatg
     5521 cggtggagaa gggcagcccg gcggaccgcg gcggcatcga ggccaccgac atcatcacgc
     5581 gcttcgacgg caagctggtc gagtcctcga gcgacctgcc gcgcctggtc ggcgccacgc
     5641 gcccgggcac cagcgtgccg gtggaggtgt ggcgcaaggc ggcggcgcgc acgctgtcgc
     5701 tcacggtggg cgagctgcag gaagacaagg tggcggcgcg cagcgcgccg cgcgagcgca
     5761 aaccggccga gctcgccgcc aaccgcctcg gcatcgcggt gagcgagctg accgccgagc
     5821 agaaacgcga gctgaagctg gggcacgggc tgatggtgac cgacgtgcgt cccgacgcgc
     5881 gcgccgaagt gcgccgcggc gacattctgc tcaagatggt gcacaacgga cagcacacgg
     5941 acctcaagtc gaacgagcag ctcaaccggc tgctcgccgg catggacaag aacgcagtga
     6001 tcacgctgca ggtgcgccgc ggcgagagca ccgccttcgt cacggtcacc ggcctggcgg
     6061 acaagggcta ggcgattctc cggctctacg ggcgcaagta ctgccacctg tgcgatgaca
     6121 tggcgcgcgc actgcgcgcc ctcggcgtgg cgttcgcgga attcgacgtc gacgccgacc
     6181 ccgagctgga ggcgcgctac ggcgagctgg tgccgctgct ggccgatgcc gcgggcgtgg
     6241 aaatctgcca ttaccacctc gacgaggcgg cgctgcgggc ccgggttgcg gtaagatagc
     6301 ggccgcttcg cacaagggtg ccgccggcac cttttttttg tgaccccgca gagccgcatc
     6361 cgcaacttct ccatcatcgc gcacatcgac cacggcaagt ccacgctggc cgaccgcatc
     6421 atccagctgt gcggcggcct ggcggaacgc gagatggccg agcaggtgct cgattcgatg
     6481 gatctggagc gcgagcgcgg catcaccatc aaggcgcaga cggcggcgct gcagtaccag
     6541 gcgcgcgacg gcgagcgcta cctgctcaac ctgatcgaca cgccggggca cgtcgatttc
     6601 tcctacgagg tgagccgctc gctcgccgcc tgcgaaggcg cgctgctggt ggtggacgcg
     6661 gcgcagggcg tcgaggcgca aaccgtggcc aactgctaca ccgcgatcga gcagggcgtc
     6721 gaggtggtgc cggtgctgaa caagatcgac ctgccatcgg ccgacccgga aaaggcgatc
     6781 caggaaatcg aggacgtcat cggcatccgc gcgcagggcg ccctgcgcgc cagcgccaag
     6841 accggcgacg gcgtgccgga gatcctcgag gcggtgatcg cgcgcattcc gccgcccaaa
     6901 ggggatcccg acgcgcgcct gcaggcgctg gtgatcgact cctggttcga caactatgtc
     6961 ggcgtggtga tgctggtgcg catgatgcag ggcaggctgc gccccaagga caaggtgctc
     7021 ctgatgagca ccggcgccgc ccacctgtgc gaacaggtcg gggtgttcac gcccaagtcg
     7081 cagccgcggg aggaactggt cgcgggggag gtcgggttcg tggtcgccgg cgtgaaggag
     7141 ctgcgcgaag cgaagatcgg cgacacgatc acccgcggcg acaggccggc cgcggcgccg
     7201 ctgccgggat tcaaggagat caagccgcag gtgttcgccg ggctctaccc ggtggaatcc
     7261 aaccagtatg aggcgctgcg cgacgcgctg gagaaact
//
LOCUS       DASRIE010000029         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1082118, whole genome shotgun sequence.
ACCESSION   DASRIE010000029 DASRIE010000000
VERSION     DASRIE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082118"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFB53_01410"
     CDS             <1..406
                     /locus_tag="VFB53_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN85417.1"
                     /translation="GATAMMPDLMSGQVPIGIVSALAGVSQAKSGKIRALAVTSAQRL
                     PNAPEIPALSETIPGFEAAPNVFLVAPAGFASERLASAVRQVVTSPEVQDSFAKQGAT
                     PMPVEPRELAARIAAEVRRWAAVVKDAGIKAE"
     gene            403..1326
                     /locus_tag="VFB53_01415"
     CDS             403..1326
                     /locus_tag="VFB53_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85418.1"
                     /translation="MNLKQLHVGFYGAVMGLAGLGLTARAAAPLFPGTFRAPAYFTEV
                     WVALGAVALAMLLPLYLLKLVRHPGAVREDFTNPLYLGFCGALPVGMSLVAGGVGAYL
                     PPLGHALWWTSFALLLAFQAWALLRWLSGGVELAKVNAGWLIILVGGIVLPGPGIGLG
                     HEEASRFVFGVSTAGAALLMPLLFYRALAGPPLAEALRPSWFILLVPPSLIYANGIAL
                     YPEARFLELLYPFALVLGVALFAYARRLARWSFGAPWWAFTFPLDALAYAAVRYAQDH
                     PFAPWRALAALALALATLFVALVLVRTLLRR"
     gene            complement(1316..>2369)
                     /locus_tag="VFB53_01420"
     CDS             complement(1316..>2369)
                     /locus_tag="VFB53_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85419.1"
                     /translation="ELRNPLGALSNASWLLQDARSDPEAEKRAREVVARQVEHLRRLT
                     DDLLDAGRAMLGKIVLHRQPLDLAAATAGVLNTLQAAGQGPVHRISRQLQPVWVAADI
                     TRVEQIVSNLVGNALKYTPVGGTITVRVARDGHEAVLTVADDGPGMPPDLVGRVFEPF
                     VQGERDLDRAHGGLGIGLTLVRRLAELHGGSASAASAGPGSGSVFQVRLPSIPAPAAV
                     VEKKAVQTSEEQRDILIIEDNADAAATLRSLLELAGHRVRVARDGQEGLDALSERLPD
                     LALIDLGLPRVDGYEVARRARAMVNGRRAPLLVAVTGYGLPEDRRRSAAAGFDEHLVK
                     PVDLGALRALLVRVSA"
BASE COUNT          271 a          864 c          846 g          388 t
ORIGIN      
        1 gggcgcgacc gcgatgatgc ccgacctgat gagcggccag gtgccgatcg ggatcgtgag
       61 cgcgctggca ggggtatcgc aggcgaagag cggcaagatc cgggcgctcg cggtcaccag
      121 cgcgcagcgc ctgccgaacg caccggagat tccggcgctg tccgagacca tccccgggtt
      181 cgaggcagct cccaatgtgt tcctggtcgc gccggcgggc tttgccagcg aacggctggc
      241 gagcgccgtg cgccaggtcg tgacctcgcc cgaggtgcag gacagctttg caaagcaggg
      301 cgccaccccg atgccggtcg agccgcgcga gctcgctgcg cgcatcgcgg ccgaggtccg
      361 gcggtgggcc gcggtggtga aggacgcggg catcaaggcg gagtgaacct aaagcagctt
      421 cacgtcgggt tctacggcgc ggtgatgggg ctcgccggcc tgggcctcac cgcgcgcgcc
      481 gccgcgccgc tctttcccgg caccttccgc gccccggcct atttcaccga agtgtgggtt
      541 gctttgggcg ccgtcgctct ggcgatgctg ctgcctcttt acctgctcaa gctcgtgagg
      601 catccgggag cggtgcgcga ggacttcacc aatccgctgt acctcggctt ctgcggggcg
      661 ctgccagtgg gcatgtcgct ggtcgcgggc ggcgtcggtg cgtatctccc cccgctgggg
      721 cacgcgctgt ggtggacgag cttcgccctg ctgctcgcgt tccaggcctg ggcgctgctg
      781 cgctggctct cgggcggcgt cgagctcgcc aaggtgaacg cgggctggct gatcatcctg
      841 gtgggcggca tcgtgctgcc cgggccgggg atcgggctcg gccacgagga ggcctcgcgc
      901 ttcgtgttcg gcgtctcgac cgcgggcgcg gcgctgctca tgccgctgct cttctatcgc
      961 gcgctcgccg gcccgccgct cgccgaggcg ctgcgcccga gctggttcat cctgctggtg
     1021 ccgccttcgc tgatctacgc caacggcatc gcgctctatc ccgaggcgcg cttcctggag
     1081 ctgctctatc cgttcgccct ggtgctcggc gtcgcgctgt tcgcctatgc gcgccggctg
     1141 gcgcgctggt ccttcggggc gccgtggtgg gccttcacct ttccgctcga cgcgctcgcc
     1201 tacgccgcgg tgcgctacgc gcaggaccat cccttcgcgc cgtggcgcgc gctcgcggcg
     1261 ctcgcgctcg cgctcgccac gctgttcgtg gcgctggtcc tggtgcgcac gctgctcagg
     1321 cgctgacccg aacgagcagg gcgcgcagcg cgcccaggtc caccggcttc accaggtgct
     1381 cgtcgaagcc ggcggccgcg ctgcggcgcc ggtcttccgg cagcccgtag ccggtgaccg
     1441 cgaccagcaa cggagcgcgt cgtccgttga ccatcgcccg cgcgcggcgc gcgacttcgt
     1501 agccgtcgac ccgcggcagg ccgagatcga tcagcgcgag gtccggcagc cgctcggaca
     1561 gggcgtcgag gccttcctgc ccgtcgcgcg ccacgcgcac gcggtggccg gcgagctcga
     1621 gcaggctgcg cagcgtggct gcggcatcgg cgttgtcctc gatgatcagg atgtcgcgct
     1681 gctcttcgga ggtctgcacg gccttttttt cgaccaccgc ggccggcgcc ggaatcgaag
     1741 gcaggcgcac ctggaacacg cttccgctcc ccggtcccgc gctcgccgcg gaggccgagc
     1801 cgccatgcag ctcggccagg cggcgcacca gcgtcagccc gatgccgagg ccgccgtgcg
     1861 cgcggtcgag gtcgcgctcg ccctgcacga agggctcgaa cacgcggccc accagatccg
     1921 gtggcatgcc ggggccgtcg tcggcgacgg tcagcaccgc ctcgtgcccg tcgcgcgcca
     1981 cgcgcacggt aatcgtgccc cctaccggcg tgtatttcag tgcgttgccg accaggttgc
     2041 tcacgatctg ctcgacccgc gtgatgtccg ctgccaccca gaccggctgg agctgccgac
     2101 tgatcctgtg gacgggacct tgccccgccg cctgaagcgt gttcaacacg ccggcagtcg
     2161 ccgccgcgag gtcgagcggc tggcggtgca gcacgatctt gcccagcatg gcgcgtccgg
     2221 catcgagcag gtcgtcggtc agccgccgca agtgctcgac ctggcgtgcg acgacttcgc
     2281 gcgcgcgctt ctctgcctca ggatcggagc gcgcgtcctg cagcagccag ctggcgttcg
     2341 acagcgcgcc cagcggattg cgcagctcg
//
LOCUS       DASRIE010000030        11151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2642923, whole genome shotgun sequence.
ACCESSION   DASRIE010000030 DASRIE010000000
VERSION     DASRIE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642923"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFB53_01425"
     CDS             <1..729
                     /locus_tag="VFB53_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZN85420.1"
                     /translation="FPFHLDFVRAAGLLLALSGALSAAPGLLRSGLASMRLAMPLALV
                     GSITSVAGAMLGLALPTQTVQTALGAAILAIALLLWRARKSEHPEVPAPDALAAALRL
                     HGVFHDPAAARDVHWRVHRTPSGLAAFAGIGLLAGLFGLGAGWANVPVLNLLMGAPLK
                     LAVGTSGFVLSVVDTAAAWVYVNRGAVLPILVAPSVIGVMLGAKIGGRLLRVTRAAVV
                     RRVVIVLLLAAGARALLKGLGLWH"
     gene            740..1138
                     /locus_tag="VFB53_01430"
     CDS             740..1138
                     /locus_tag="VFB53_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85421.1"
                     /translation="MTPPEQLLYARWLDWATRAGLALLVASFAAYALGLAAPFVPFEE
                     LARAWSLPVDRYRAAVGAPAGWGWLALLRHGDYMNFAGIALLALASLACTLRVLPALL
                     VRGERALALIGLLQAAVLALAAAGVFGGAH"
     gene            1210..2220
                     /locus_tag="VFB53_01435"
     CDS             1210..2220
                     /locus_tag="VFB53_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN85422.1"
                     /translation="MKAVHSIAAGAAAALFSGALCAQSTLDAVKSKGYVQCGVNTGLA
                     GFSQPDSRGAWKGLDVDTCRAVAAAVFGDHAKVRYTPLTAQQRFTALQSGEVDILSRN
                     TTWTITRDTSLGLNFAAVTYYDGQGFMVPKKLNVKSAKQLNGATICVQPGTTTELNLA
                     DYFRANRMSFKPVVIEKLEEVTNAYFAGRCDVFTTDVSGLVSTRGSRAANPADHLILP
                     EVISKEPLGPVVRHGDDRWFDVVKWAVFAMLEAEELGLSSKNIDQNASSTNPAVQRFV
                     GATGDIGKMLALDNRWAYNIVKQVGNYGESFEANLTPLGFDRGINNLWTKGGLMYAPP
                     VR"
     gene            2230..3369
                     /locus_tag="VFB53_01440"
     CDS             2230..3369
                     /locus_tag="VFB53_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168893.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN85423.1"
                     /translation="MRARAAAVQLALLAAVAFVLWYLASNTAANLEARKIASGFAFLG
                     REAGFEIGEAPFLGYGAADSYARAIVVGLINTFRVAVIGIALATLLGMVLGLARLSRN
                     WLLAKFAAGYIEVVRNVPLLVQLFFLYAVITENLPGPREALSPLAGVYFSNRGIAFPL
                     PAPHAAHAWVLAALLAGAVLAWGLARWARARQARTGQPFPVLGAGAALVLGLPLAVFL
                     AAGAPLALARPEMQGFNFAGGAILTPEFATLLFGLTLYTAAFIAEIVRAGVQAVDRGQ
                     SEAAYSLGLPRSRTTRLVILPQALRVIVPPLTSQYLNLTKNSSLAVAIGYPDLVSIAN
                     TTMNQTGQAIEGIAIIMAVYLTISLSISALMNWYNARIALKGARR"
     gene            3366..4421
                     /locus_tag="VFB53_01445"
     CDS             3366..4421
                     /locus_tag="VFB53_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZN85424.1"
                     /translation="MRWLRENLFGSWASALATLAILWLAWTLLPPFVEWAFLKAVWIA
                     PDSRPCRADGAGACWALIAEKHRFILFGTYPYEQHWRPGAAILLLFALYAASALRRFW
                     RPALALAWIAGLALIGVLMWGGVAGLPYVENERWGGLVLTLLLATFGLAFAFPISILL
                     ALGRRSQLPAVRALSVAWIELVRGVPLISLLFMASVMLPLFLPEGVSIDKLLRALIAM
                     IVFAAAYLAEVVRGGLQAIPHGQLEAADALGLGYWRKTLLVVLPQALRIAIPPLVNTF
                     IGLFKDTSLVVIIGLFDLLSSIKVALGEPAWSGFGVEAYLFASLVYFVFCYAMSRYSQ
                     NLERELERSRTHVLKGG"
     gene            4423..5166
                     /locus_tag="VFB53_01450"
     CDS             4423..5166
                     /locus_tag="VFB53_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZN85425.1"
                     /translation="MAGKPMIEMRGVGKWFGAFQVLKDVELGVAQGEKIVLCGPSGSG
                     KSTLVRLVNGLEPYQAGKVVVDGVELGNDPRAIEAVRLEVGMVFQSFNLFPHLTALEN
                     LTLAPIWVRRMPKKDAEAQALQLLERVRIGEQAHKYPAQLSGGQQQRVAIARALAMKP
                     KIMLFDEPTSALDPEMIKEVLDVMVELAREGMTMVVVTHEMGFARTVADRVCFMDHGE
                     IVETAAPREFFEAPKTERARVFLSQILGH"
     gene            5217..5804
                     /locus_tag="VFB53_01455"
     CDS             5217..5804
                     /locus_tag="VFB53_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZN85426.1"
                     /translation="MGAIFQSLGRTVLAGVVLVVIMLGVAGTFGRMGEHGYWLFFMRW
                     LHVLSAVMWIGLLWYFNFVQIPSMPKIPDEQKPAIGKVIAPAALFWFRWAALATVVTG
                     LLLAGMHKGYLVNALSLQAPHQAIGVGMWLGLVMAFNVWFIIWPNQKKALGMVQVTPE
                     EKAAAARMAMLTSRANTMLSIPMLYCMVAQQNAGL"
     gene            complement(5805..6239)
                     /locus_tag="VFB53_01460"
     CDS             complement(5805..6239)
                     /locus_tag="VFB53_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZN85427.1"
                     /translation="MRTLTLALLLGAAPAQAQQPPLPTVELGAGMHLIRAELAATDAA
                     RAAGLMHRRSLAPNGGMLFVFDAPGAYCMWMKNTPLPLSVAFIDAAGAIVNIADMQPH
                     TEQSHCAAGPVRYALEMRQGWFAERGIRAGERLRGLEKLKPR"
     gene            complement(6294..6482)
                     /locus_tag="VFB53_01465"
     CDS             complement(6294..6482)
                     /locus_tag="VFB53_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85428.1"
                     /translation="MNKFLTVVAAAMFAAVSASAFAASHAGAPMKDGAKAEKKADKKA
                     APKKAPAKKAEKKDEKKK"
     gene            6638..7792
                     /locus_tag="VFB53_01470"
     CDS             6638..7792
                     /locus_tag="VFB53_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85429.1"
                     /translation="MSRLLLSVLLCLAAAPATAAPRLPAEDREVLERLPLRRSDPAAA
                     ELRALREAARAAPADPAAVVPLARRYFALAQSEGDPRYVGYAEAALAPWRAAADAPAE
                     VLVQRALLRQYRHDFDGALADLALALARDPLSAEARAWRAAIFMVRADYAAAARECAA
                     LDESLLAAGCSAYVAAATGGTRAAYQGLRAALERAPRADPGERLWVLTRLAEMAARLQ
                     DPAAAERHFRAALALGRDDNFLLAAYADFLLERGRPAEAAALLRDWARADTLLLRLAL
                     AERALGLPGAARHAQELGERFAAAALRGDRLHLAEEARYLLELRGDARAAVAAAAENW
                     KSQREPRDALVLLRAARAAGDRAAAAPALDWLARSRFEDERLRRLAEALR"
     gene            7789..8907
                     /locus_tag="VFB53_01475"
     CDS             7789..8907
                     /locus_tag="VFB53_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZN85430.1"
                     /translation="MRRALLLALALACAPALAHKPSDSYLALAVEDGALRGQWDIALR
                     DLEHAIGLDADGDGAITWGELRARHAELAAYALARLALAADGRPCATRAREHLVDAHS
                     DGAYAVLRFAADCGAAPQRRLEVGYSLFADLDPTHRGLVRVQHGGATASAVLGPERAR
                     LEVALGETSRRAQFLDYAREGVWHIWIGIDHVLFLLSLLLPAVLVYGARAWAPAERFA
                     PVFWDVFKIVTAFTVAHSITLSVAALGLVALPSRLVESVIALSVVLAALNNLWPVVLA
                     RRWAVAFGFGLVHGFGFASVLADLGLPRDALLVSLVGFNLGVEAGQLAIVALFLPAAF
                     AARRHWAYRRLVLAGGSAAIALVALAWMAERIFDLKLL"
     gene            8943..10199
                     /gene="icd"
                     /locus_tag="VFB53_01480"
     CDS             8943..10199
                     /gene="icd"
                     /locus_tag="VFB53_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZN85431.1"
                     /translation="MAYQHIKVPSAGEKIRVNKDYSLAVPDQPIIPFIEGDGTGVDIT
                     PVMIKVVDAAVKKAYGAKKKIVWMEMYAGEKSCRVYGENVWLPEETLAAAKEYVVSIK
                     GPLTTPVGGGIRSINVALRQELDLYVCLRPIRYFKGVPSPLKEPEKTDMVIFRENAED
                     IYAGIEWAAKTPEAQKVVRFLIDEMKVKKIRFPDTSAIGIKPVSIQGSERLIRKAFQY
                     AVDNDRRSVTLVHKGNIQKFTEGGFRDWGYALAQKEFGAKPVDGGPWCSFRNPKSGRE
                     IAVKDVITDAFLQQILLRPAEYDVIATLNLNGDYVSDALAAQVGGIGIAPGANMSDSI
                     AMFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHLGWVAAADAIVKSMEKAIADKVVT
                     YDFARLMQGARQVSCSGFGQAMIERM"
     gene            complement(10264..10467)
                     /locus_tag="VFB53_01485"
     CDS             complement(10264..10467)
                     /locus_tag="VFB53_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN85432.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMAGFKTLKE
                     GMKVSFDVVQGPKGKQASNINKA"
     gene            10609..10914
                     /gene="clpS"
                     /locus_tag="VFB53_01490"
     CDS             10609..10914
                     /gene="clpS"
                     /locus_tag="VFB53_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZN85433.1"
                     /translation="MAQSRDDTLLEARRSKLRPPPMFKVMLLNDDFTPMEFVVVVLQK
                     FFGMTRERATQVMLKVHKEGIGVCGVFPQDIASTKVQQVAAYARRHQHPLQCVMEET"
     gene            10916..>11151
                     /locus_tag="VFB53_01495"
     CDS             10916..>11151
                     /locus_tag="VFB53_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZN85434.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACGANLDELRKSLGQHVAEQTPRIAAEREVDTQP"
BASE COUNT         1502 a         3937 c         3936 g         1776 t
ORIGIN      
        1 tttcccttcc acctggattt cgtgcgcgcc gccggactcc tgctcgcgct ctcgggagcg
       61 ctgtcggccg ccccgggcct gctgcgcagc ggcctggcga gcatgcgcct cgccatgccg
      121 ctcgccctgg tcggctcgat cacctcggtc gcgggcgcga tgctggggct cgcgctgccc
      181 acccagacgg tgcagaccgc gctcggcgcc gccatcctcg ccatcgcgct gctgctgtgg
      241 cgcgccagga agtccgagca cccggaggtg ccggcgcccg acgcgctggc cgcggcattg
      301 cggctgcacg gcgtgttcca cgatccggcg gctgcccgcg acgtgcactg gcgcgtgcat
      361 cgcaccccgt cgggcctcgc cgcgttcgcc ggcatcgggc tgctcgccgg cctgttcggc
      421 ctgggggcgg gctgggccaa cgtccctgtg ctcaacctgc tgatgggagc gccgctcaag
      481 ctcgcggtcg gcacctccgg cttcgtcctg tcggtggtcg acaccgcggc ggcctgggtc
      541 tacgtcaacc gcggcgcggt gctgccgatc ctggtggcgc cttccgtcat cggcgtgatg
      601 ctgggcgcga agatcggcgg ccgcctgctg cgcgtgacgc gcgccgcagt ggtgcgccgc
      661 gtggtgatcg tgctgctgct cgccgccggc gcgcgcgcgc tgctcaaggg attggggcta
      721 tggcattgag gcgctcgccg tgacgccgcc ggagcagctg ctgtacgcgc gctggctcga
      781 ctgggccacg cgggcaggcc tcgccctgct ggtcgccagc ttcgccgcct acgcgctcgg
      841 gctggccgcg cccttcgtgc ctttcgagga actggcgcgc gcctggtcgc tgccggtgga
      901 ccggtaccgc gccgccgtgg gcgccccggc cggctggggc tggctggcgc tcctgcgcca
      961 cggcgactac atgaatttcg ccggcatcgc gctcctcgcc ctggcctcgc tcgcctgcac
     1021 gctccgggtg ctgccggcgc tcctcgtccg gggcgagcgc gcactcgccc tgatcggcct
     1081 cctgcaggcc gccgtgctgg cgctggccgc ggccggcgtg ttcggcggcg cgcactgaaa
     1141 gcgcgccacc gttgcgcgaa gcgcggcgcc cggcatagac tgcctgccac acaacggagg
     1201 agggtttcca tgaaagcagt ccacagcatc gccgccggcg ccgccgccgc cctgttcagc
     1261 ggcgcgctct gcgcgcaatc gacgctcgac gcggtgaaat ccaagggcta cgtgcagtgc
     1321 ggcgtcaaca ccggcctcgc cggcttctcg cagccggatt cgcgcggcgc ctggaagggc
     1381 ctggacgtcg atacctgccg cgcggtggcc gccgccgtgt tcggcgacca cgcgaaggtg
     1441 cgctacacgc cgctcaccgc gcagcagcgc ttcaccgcgc tgcagtcggg cgaagtcgac
     1501 atcctgtcgc gcaataccac ctggaccatc acccgcgaca ccagcctcgg cctgaacttc
     1561 gccgccgtca cctactacga cggccagggc ttcatggtgc cgaagaagct caacgtgaaa
     1621 agcgccaagc agctgaacgg cgccaccatc tgcgtgcagc ccggcaccac caccgagctc
     1681 aacctcgccg actacttccg cgccaaccgc atgagcttca agccggtggt gatcgagaag
     1741 ctggaggagg tcaccaacgc ctacttcgcc gggcgctgcg acgtgttcac caccgacgtg
     1801 tccggcctgg tgtcgacgcg cggctcgcgc gccgccaacc cggcggacca cctgatcctg
     1861 cccgaggtga tctccaagga gccgctcggg ccggtggtgc gccacggcga cgaccgctgg
     1921 ttcgacgtcg tcaagtgggc cgtgttcgcc atgctcgagg ccgaggaact gggcctcagc
     1981 tcgaagaaca tcgaccagaa cgcctcgagc accaaccccg cggtgcagcg cttcgtcggc
     2041 gccaccggcg acatcggcaa gatgctggcg ctggacaacc gctgggccta caacatcgtc
     2101 aagcaggtcg gcaactacgg cgagtccttc gaggccaacc tgacgccgct cggcttcgac
     2161 cgcggcatca acaacctgtg gaccaagggc ggcctgatgt acgcgccgcc cgtgcgctaa
     2221 agcgccccgg tgcgcgcgcg cgccgccgcc gtccagctcg cactgctggc ggcggtggcg
     2281 ttcgtcctgt ggtacctcgc cagcaacacc gcggcgaacc tggaggcgcg caagatcgcg
     2341 agcggcttcg cctttctcgg gcgcgaggcc gggttcgaga tcggcgaagc gccgttcctc
     2401 ggctacggcg cggccgacag ctacgcgcgc gcgatcgtcg tcgggctgat caacaccttc
     2461 cgcgtcgccg tgatcggcat cgcgctcgcc acgctgctcg gcatggtgct cggcctcgcc
     2521 cgcctgtcgc gcaactggct gctggcgaag ttcgccgcgg gctacatcga ggtggtgcgc
     2581 aacgtgccgc tgctggtgca gctgttcttc ctctacgcgg tgatcactga gaacctgccc
     2641 gggccgcgcg aggcgctcag ccccctggcg ggggtgtact tcagcaaccg cggcatcgcc
     2701 tttcccctgc cggcgccgca cgcggcgcac gcctgggtgc tcgccgcgct gctggcgggc
     2761 gccgtgctcg cctgggggct ggcgcgctgg gcgcgcgcgc ggcaggcgcg cacggggcag
     2821 cccttcccgg tgctcggcgc cggcgcggcg ctggtgctgg ggctgccgct cgccgtgttc
     2881 ctcgccgccg gcgcgccgct cgcgctggcg cgtcccgaga tgcagggctt caacttcgcc
     2941 ggcggcgcca tcctcacgcc cgagttcgcc acgctgctct tcggcctcac gctgtacacc
     3001 gccgcgttca tcgccgagat cgtgcgcgcc ggcgtgcagg cggtggaccg cggccagtcc
     3061 gaggccgcct actcgctcgg cctgccgcgc agccgcacca cgcggctggt gatcctgccg
     3121 caggcgctgc gcgtcatcgt gccgccgctc accagccagt acctcaacct caccaagaac
     3181 agctcgctgg cggtggccat cggctatccc gacctggtgt cgatcgccaa caccaccatg
     3241 aaccagaccg gacaggcgat cgagggcatc gccatcatca tggcggtgta cctgacgatc
     3301 agcctgtcga tctcggcgct gatgaactgg tataacgcgc gcatcgcgct gaaaggggcg
     3361 cggcggtgag gtggctgcgc gagaacctgt tcggcagctg ggcgagcgcg ctcgccacgc
     3421 tcgccatcct gtggctcgcc tggacgctgc tgccgccgtt cgtcgagtgg gcgttcctca
     3481 aggcggtgtg gatcgccccg gattcgcgcc cctgccgcgc cgacggcgcg ggcgcctgct
     3541 gggcgctgat cgcggagaag caccgcttca tcctgttcgg cacctacccc tacgagcagc
     3601 actggcggcc cggcgcggcg atcctgctgc tgttcgcgct gtacgcggcg agcgcgctgc
     3661 gccgcttctg gcgcccggcg ctcgcgctcg cctggatcgc gggcctcgcg ctcatcggcg
     3721 tgctgatgtg gggcggggtc gccggcctgc cctacgtgga gaacgagcgc tggggcggcc
     3781 tggtcctcac gctgctgctc gccaccttcg gcctggcgtt cgccttcccc atctccatcc
     3841 tgctcgcgct cggccgccgc tcgcagctgc ccgcggtgcg cgcgctgtcg gtcgcctgga
     3901 tcgagctggt gcgcggcgtg ccgctgatct cgctcctgtt catggcctcg gtgatgctgc
     3961 cgctgttcct gcccgagggc gtcagcatcg acaagctgct gcgcgcgctc atcgccatga
     4021 tcgtgttcgc cgccgcctac ctggccgagg tggtgcgcgg cggcctgcag gcgatcccgc
     4081 acgggcagct cgaggccgcg gacgcgctcg gcctgggcta ctggcgcaag acgctgctgg
     4141 tggtgctgcc gcaggcgctg cgcatcgcca tcccgccgct ggtgaacacc ttcatcggcc
     4201 tgttcaagga tacctcgctg gtggtgatca tcggcctgtt cgacctgctc agctcgatca
     4261 aggtggcgct cggcgagccg gcctggagcg ggttcggcgt cgaggcgtac ctgttcgcgt
     4321 cgctggtcta tttcgtgttc tgctacgcca tgtcgcgcta cagccagaac ctggagcgcg
     4381 agctggagcg cagccgcacg cacgtgctga aggggggatg agatggccgg caagccgatg
     4441 atcgagatgc gcggcgtggg caagtggttc ggcgccttcc aggtgctgaa ggacgtcgag
     4501 ctgggcgtgg cgcagggcga gaagatcgtg ctgtgcgggc cttcgggctc gggcaagtcc
     4561 accctggtgc gcctggtgaa cggcctggag ccctaccagg cgggcaaggt ggtggtcgac
     4621 ggcgtcgagc tgggcaacga cccgcgcgcc atcgaggcgg tgcgcctcga ggtcggcatg
     4681 gtgttccaga gcttcaacct gttcccgcac ctcaccgcgc tggaaaacct gacgctcgcg
     4741 ccgatctggg tgcggcgcat gccgaagaag gacgccgagg cgcaggcgct gcagctgctg
     4801 gagcgcgtgc gcatcggcga gcaggcgcac aagtacccgg cgcagctctc cggcggccag
     4861 cagcagcgcg tggcgatcgc gcgcgcgctg gcgatgaagc ccaagatcat gctgttcgac
     4921 gagccgacct cggcgctcga cccggaaatg atcaaggaag tgctcgacgt gatggtcgag
     4981 ctggcgcgcg agggcatgac catggtggtg gtgacccacg agatgggctt cgcccgcacg
     5041 gtggccgacc gcgtgtgctt catggaccac ggcgagatcg tcgagaccgc ggcgccgcgc
     5101 gagttcttcg aggcgccgaa gaccgagcgc gcgcgggtgt tcctcagcca gatcctcggg
     5161 cactagctgt gctagacttt tttcgtcctc cataagagtc aaccaaagga gacatcatgg
     5221 gcgccatctt ccagtccctc ggccgcaccg tcctcgccgg ggtcgttctc gtggtcatca
     5281 tgctcggcgt ggccggcacc ttcggccgga tgggcgagca cggctactgg ctgttcttca
     5341 tgcgctggct gcacgtgctg tcggcggtga tgtggatcgg cctgctgtgg tatttcaact
     5401 tcgtgcagat cccctcgatg cccaagatcc cggacgagca gaagccggcg atcggcaagg
     5461 tgatcgcgcc cgccgcgctg ttctggttcc gctgggcggc gctcgccacc gtggtgaccg
     5521 ggctgctgct cgccggcatg cacaagggct acctggtgaa tgcgctcagc ctgcaggcgc
     5581 cgcaccaggc gatcggcgtc ggcatgtggc tgggcctggt gatggccttc aacgtgtggt
     5641 tcatcatctg gccgaaccag aagaaggcgc tcggcatggt gcaggtgacg cccgaggaaa
     5701 aggcggcggc ggcgcgcatg gcgatgctca cttcgcgcgc caacaccatg ctgtcgatcc
     5761 ccatgctgta ctgcatggtg gcgcagcaga acgccggact gtgatcagcg cggcttgagc
     5821 ttttccagcc cgcgcaggcg ctcgccggcg cggatgccgc gctcggcgaa ccagccctgg
     5881 cgcatctcca gcgcgtagcg caccgggcca gccgcgcagt gcgactgctc ggtgtgcggc
     5941 tgcatgtcgg cgatgttgac gatggcgccc gcggcgtcga tgaacgcgac cgacaggggc
     6001 agcggcgtgt tcttcatcca catgcagtac gcgcccggcg cgtcgaacac gaacagcatg
     6061 ccgccgttgg gggcgagcga ccggcggtgc atcagcccgg ccgcacgcgc ggcgtccgtg
     6121 gcggcgagct cggcgcggat caggtgcatg cccgcgccca gctccaccgt cggcaggggc
     6181 ggctgctgcg cctgcgccgg ggcggcgccc agcagcagcg cgagggtcag ggtgcgcatg
     6241 ggcgaaaaaa aagggcaggg taacttccct gccctgtttc cggaagtgcc ggattacttc
     6301 ttcttctcgt ccttcttctc ggccttcttg gcaggcgctt ttttcggcgc cgctttcttg
     6361 tccgccttct tctcggcttt cgccccgtcc ttcatcggcg cgcccgcatg cgatgcggcg
     6421 aaggcggagg cgctgacggc cgcgaacatc gctgcggcga ctacggtgag gaacttgttc
     6481 attctttcat ctcctagtag gtgtccagac aggtagcgca gcgggaacgc cacgctccag
     6541 taaccgttaa cgcggcattt ccgcaccggt tgacaggctc ccgccagcta tcatttaagc
     6601 aaatgatatt attgggtttt ccctagaccc tgccgccttg tcccggctcc tgctgagcgt
     6661 cctgttgtgc cttgcggcag cgccggcgac ggccgcgccc cgcctgccgg cggaggaccg
     6721 ggaagtgctc gagcgcctgc ccctgcgccg atcggacccc gcggccgccg agctgcgcgc
     6781 gctgcgcgag gcggcgcgcg ccgcgcccgc ggatcccgcg gcggtggttc ccctggcgcg
     6841 ccgctatttc gcgctcgccc agtccgaggg cgacccgcgc tacgtgggct atgccgaggc
     6901 cgcgcttgcg ccctggcgcg ccgcggcgga cgccccggcg gaagtcctgg tgcagcgcgc
     6961 gctcctgcgc cagtaccggc acgatttcga cggcgcgctc gccgacctcg cgctggcgct
     7021 cgcgcgcgac ccgctgtccg ccgaggcgcg cgcctggcgc gcggcgatct tcatggtgcg
     7081 cgccgactac gccgcggcgg cgcgcgagtg cgccgcgctg gacgaatcgc tgcttgccgc
     7141 cggctgcagc gcctacgtcg ccgccgccac gggcggcacg cgcgccgcct accaggggct
     7201 gcgcgccgcg ctcgagcgcg cgccgcgggc ggacccgggc gagcgcctct gggtgctgac
     7261 gcgcctggcg gagatggcgg cgcgcctgca ggacccggcg gcggccgagc gccacttccg
     7321 cgccgcgctg gcgctcggcc gcgacgacaa tttcctcctc gcggcatacg ccgacttcct
     7381 gctcgagcgg ggccggcccg cggaggccgc cgccctgctc agggactggg cgcgcgccga
     7441 caccctgctg ctgcgcctcg cgctggcgga acgggcgctc ggcctgcccg gggccgcgcg
     7501 ccatgcgcag gaactgggcg agcgcttcgc ggccgccgcg ctgcgcggcg accggctgca
     7561 cctcgcggag gaggcgcgct acctgctcga gctgcgcggc gacgcgcgcg cggcggtggc
     7621 cgccgccgcc gagaactgga agtcgcagcg cgagccgcgc gacgcgctgg tcctgctgcg
     7681 ggccgcccgc gccgccgggg accgggcggc ggcggccccc gcgctcgact ggctggcgcg
     7741 ctcgcgcttc gaggatgaac gcctgcgccg cctggcggag gcgctgcggt gaggcgcgcc
     7801 ctgctgctcg ccctggcgct cgcctgcgcg ccggcgctgg cgcacaagcc gagcgacagc
     7861 tacctggcgc tcgccgtcga ggacggcgcg ctgcgcggcc agtgggacat cgccctgcgc
     7921 gacctggagc acgccatcgg cctggacgcc gacggcgacg gcgccatcac ctggggcgag
     7981 ctgcgcgcgc gccacgccga gctcgccgcc tacgcgctgg cgcgcctcgc gctcgccgcc
     8041 gacggccgtc cctgcgcgac ccgggcgcgc gagcacctgg tggatgcgca cagcgacggc
     8101 gcctatgccg tgctgcgctt cgcggcggat tgcggcgccg cgccgcagcg gcgcctggag
     8161 gtcggctaca gcctgttcgc cgacctcgat cccacgcacc gcggcctggt gcgcgtgcag
     8221 cacggcggcg cgacggcgag cgcggtgctc ggcccggagc gcgcgcgcct ggaggtggcg
     8281 ctgggcgaga cttcgcgccg cgcccagttc ctcgactacg cgcgcgaggg cgtctggcac
     8341 atctggatcg gcatcgacca cgtgctgttc ctgctgtcgc tgctgctgcc ggcggtgctg
     8401 gtctacggcg cgcgcgcctg ggcgccggcc gagcgcttcg ccccggtgtt ctgggacgta
     8461 ttcaagatcg tcaccgcgtt caccgtcgcg cactcgatca cgctgtcggt ggcggcgctc
     8521 ggcctggtcg cgctgccgtc gcggctggtc gaatcggtga tcgccctgtc ggtggtcctg
     8581 gcggcgctca acaacctctg gccggtggtg ctggccaggc gctgggcggt ggcgttcggc
     8641 ttcgggctgg tgcacggctt cggcttcgcc agcgtgctcg ccgacctcgg cctgccgcgc
     8701 gatgcgctgc tcgtctcgct ggtcggcttc aacctcggcg tcgaagccgg ccagctggcg
     8761 atcgtcgccc tgttcctgcc ggcggccttc gccgcgcgcc ggcactgggc ctaccggcgc
     8821 ctggtgctcg ccggcggctc ggccgccatc gccctcgtcg ccctggcgtg gatggcggaa
     8881 cggattttcg acctcaagct cttataatcc gcacttttcg gcgcgaagcc gcaggagtat
     8941 ccatggcgta ccagcacatc aaggtcccct cggcgggcga gaagatccgg gtcaacaagg
     9001 actactcgct cgcggtgccg gaccagccca tcattccctt catcgagggc gacggcaccg
     9061 gggtggacat cacgccggtg atgatcaagg tggtggacgc ggcggtgaag aaggcctacg
     9121 gcgcgaagaa gaagatcgtg tggatggaga tgtacgcggg cgagaagtcc tgcagggtct
     9181 acggcgagaa cgtgtggctg cccgaggaga cgctcgccgc cgcgaaggag tacgtggtgt
     9241 cgatcaaggg accgctcacc acgccggtcg gcggcggcat ccgctcgatc aacgtggcgc
     9301 tgcgccagga actggacctg tacgtctgcc tgcggccgat ccgctacttc aagggcgtgc
     9361 cctcgccgct gaaggagccg gagaagaccg acatggtgat cttccgcgag aacgcggaag
     9421 acatctacgc cggcatcgag tgggcggcga agacccccga ggcacagaag gtggtcaggt
     9481 tcctcatcga cgagatgaag gtgaagaaga tccgcttccc cgacacctcg gcgatcggca
     9541 tcaagccggt ctccatccag ggctcggagc gcctgatccg caaggccttc cagtacgccg
     9601 tcgacaacga ccgcaggtcg gtgaccctgg tgcacaaggg caacatccag aagttcaccg
     9661 agggagggtt ccgcgactgg ggctatgcgc tggcgcagaa ggagttcggc gccaagccgg
     9721 tcgacggcgg gccctggtgc agcttcagga acccgaagag cgggcgcgag atcgcggtga
     9781 aggacgtgat caccgacgct ttcctgcagc agatcctgct gcgcccggcg gaatacgacg
     9841 tgatcgccac gctgaacctg aacggcgact acgtgtcgga tgcgctcgcc gcgcaggtcg
     9901 gcggcatcgg catcgcgccg ggggccaaca tgagcgattc gatcgcgatg ttcgaggcca
     9961 cccacggcac cgcgcccaag tatgccggca aggactacgt caacccgggc tcggagatcc
    10021 tgtcggcgga gatgatgctg cgccacctgg gctgggtggc ggctgccgac gcgatcgtga
    10081 agtcgatgga aaaggcgatc gccgacaagg tggtgaccta cgacttcgcg cgcctgatgc
    10141 agggcgccag gcaggtctcc tgctccggct tcggccaggc gatgatcgag cgcatgtagc
    10201 gcgccccgcg ggcgaagaaa aagccgcccg cgggcggctt tttttgcgtc gcggcggagg
    10261 cgttcaggcc ttgttgatgt tcgaggcctg cttgcccttg ggtccctgca ccacgtcgaa
    10321 gctgaccttc atgccctcct tgagggtctt gaagcccgcc atctggatcg cggaaaagtg
    10381 cgcgaacagg tcctcgctgc cgtcgtccgg cgtgatgaag ccgtagccct tcgcgtcgtt
    10441 gaaccacttc acggtgccgg tcgccatcgc cgctgccctc gggtgcacaa aaggcggctt
    10501 gtacgcgcct tcgattagcc agtcaagacc ccccgggagc cgcgttgcac ggcgggtata
    10561 cattgattag gatagctgcg acgaggatga cagcgacaac cacgcggcat ggcgcagtcc
    10621 agggacgaca ctctgctcga ggcacggcgg agcaagctgc ggccgccgcc gatgttcaag
    10681 gtgatgctgc tgaacgacga tttcacgccg atggagttcg tggtggtggt gctgcagaag
    10741 ttcttcggca tgacgcgcga gcgcgcgacg caggtgatgc tgaaggtgca caaggagggc
    10801 atcggcgtgt gcggggtgtt tccgcaggac atcgcctcca ccaaggtgca gcaggtcgcg
    10861 gcgtacgcgc ggcggcacca gcatccgctg cagtgcgtga tggaggaaac gtaggatgat
    10921 cgcccaggaa ctcgaagtca gcctgcacat ggcgttcgtc gaggcgcgcc agaagcggca
    10981 cgagttcatc accgtcgagc acctgctgct cgcgctgctc gacaacccga ccgcggccga
    11041 ggtgctgcgc gcctgcggcg ccaacctgga cgaattgcgc aagagcctgg gccagcacgt
    11101 cgccgagcag acgccgcgca tcgccgccga gcgcgaggtg gacacgcagc c
//
LOCUS       DASRIE010000031         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1590614, whole genome shotgun sequence.
ACCESSION   DASRIE010000031 DASRIE010000000
VERSION     DASRIE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590614"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFB53_01500"
     CDS             <1..485
                     /locus_tag="VFB53_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN85435.1"
                     /translation="AVMDFSMPGRSGFDLLGDLRREFPARPVIVLSIHAEQLHAARVL
                     KAGGAGYLNKSSAPQELAKAVRKVAAGGRYVSAALAEMLASDLSSDAGGPPHEQLSDR
                     EYRVMWLLASGRQINQIARELLLRPSTVSTYRTRVFRKLHLRNNAELVQYAVRHQLVE
                     "
     gene            494..1603
                     /locus_tag="VFB53_01505"
     CDS             494..1603
                     /locus_tag="VFB53_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN85436.1"
                     /translation="MDAPAKANILLVDDERRNLVALRELLAGPGRSLLLAGSGEEALR
                     LALQHDFAVVLLDVRMPRIDGFETARLLRARERSRLTPVIFLTAAADEFESMFRGYEV
                     GAVDYLVKPLVPEVLRSKVAVFVELHAKSAALAREIAERRGAEQRLVESEQRLRALAA
                     HLVSVREEERASIAREIHDELGQVLTGLKMDVTWLAARLEAKPLLQKTASMCELIDAT
                     IRAVRRISAGLRPEVLDDMGLLAGIGWQAKEFQKRTGIRCRVKLPAEGAGFGSELSIN
                     AFRIFQEILTNVARHAKATRVEVALSAAPDRLVLEVADNGVGIATGKVNGKGSFGLLG
                     MRERALRLGGELSIDSARRQGTRITVSLPCGEAGA"
     gene            1600..2229
                     /locus_tag="VFB53_01510"
     CDS             1600..2229
                     /locus_tag="VFB53_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN85437.1"
                     /translation="MKVLIADDHPVVRRGLRQMLDAESDLSVVVEAKDGHEVIDQSAR
                     VPWDVAVLDYNMPGKGGIELVKELRRRYPDRPVLVLSMYPEDRYALRLLKAGAAGYLN
                     KESAADELVGALRKVAGGGRYVSAALGEKLAQAMAGDGDAPAYEKLSDREYQIMWLIA
                     SGKTVGEVARQLFLSPNTVSTYRSRIFAKLGVKNNSELMRYAIRHQLVE"
     gene            2246..>3043
                     /locus_tag="VFB53_01515"
     CDS             2246..>3043
                     /locus_tag="VFB53_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZN85438.1"
                     /translation="MQTIDNGLQAVLDRGELLRALRAYRRGDFAVRMPLTLPGIDGDI
                     AQAFNEVVEMNAALAAEMARVSDQVGKEGRVGQRVGLHGASGAWAGCVDSVNTLIGDV
                     AYPVTEVARVIGSVAKGDLSQTMVLEVDGRALRGDVLRIATVVNTMVGQLNAFASEVT
                     RVAREVGTDGKLGGQAQVPGVAGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVAKGDL
                     SKKITVDVKGEILELKSTINTMVDQLGAFASEVTRVAREVGTDGKLGG"
BASE COUNT          465 a         1011 c         1163 g          404 t
ORIGIN      
        1 tcgcggtgat ggatttctcc atgcccgggc gcagcggctt cgacctgctg ggcgacctgc
       61 ggcgcgagtt tcccgcgcgc ccggtgatcg tgctcagcat ccacgccgag cagctgcacg
      121 cggcgcgcgt gctgaaggcg ggcggcgcgg gctacctgaa caagtcgagc gcgccgcagg
      181 agctggcgaa ggcggtgcgc aaggtggccg ccggcggccg ctacgtgagc gcggcgctcg
      241 ccgagatgct ggcctccgac ctgtccagcg acgcgggcgg gccgccgcac gagcagcttt
      301 ccgaccgcga ataccgcgtc atgtggctgc tcgcctccgg gcggcagatc aaccagatcg
      361 cgcgcgagct gctgctgcgc cccagcaccg tgagcaccta ccgcacgcgc gtgttccgca
      421 agctgcactt gcgcaacaac gccgagctgg tgcagtacgc ggtgcgccac cagctcgtcg
      481 agtagcgcgc cgcatggacg cgcccgccaa ggcgaacatc ctgctggtcg acgacgagcg
      541 gcgcaatctc gtcgccctgc gcgagctgct ggccgggccg ggccgcagcc tgctgctcgc
      601 cggctcgggc gaggaggcgc tgcgcctcgc gctgcagcac gatttcgccg tggtgctgct
      661 cgacgtgcgc atgccgcgca tcgacggctt cgagaccgcg cggctgctgc gcgcgcgcga
      721 gcgctcgcgc ctcacgccgg tgatcttcct caccgccgcc gccgacgagt tcgagtcgat
      781 gttccgcggc tacgaggtgg gggcggtgga ctacctggtg aagccgctgg tgcccgaggt
      841 gctgcgctcc aaggtcgcgg tgttcgtcga gctgcacgcc aagagcgccg cgctggcgcg
      901 cgaaatcgcc gagcgccgcg gcgccgagca gcgcctggtc gagtcggagc agcgcctgcg
      961 cgcgctggcg gcgcacctgg tgtcggtgcg cgaggaggag cgcgccagca tcgcgcgcga
     1021 gatccacgac gagctcggcc aggtgctcac cgggctgaag atggacgtca cctggctggc
     1081 ggcgcggctg gaggcgaagc cgctgctgca gaagaccgcg tcgatgtgcg agctgatcga
     1141 cgccaccatc cgcgccgtgc ggcgcatctc cgccgggctg cggcccgagg tgctcgacga
     1201 catgggcctg ctggcgggca tcggctggca ggcgaaggaa ttccagaagc gcaccggcat
     1261 ccgctgccgg gtcaagctgc ccgccgaggg cgcgggcttc ggctccgagc tctcgatcaa
     1321 cgccttccgc atcttccagg agatcctcac caacgtcgcg cgccacgcca aggcgacgcg
     1381 cgtcgaggtg gcgctgagcg cggcgcccga ccggctggtg ctggaagtgg ccgacaacgg
     1441 cgtgggcatc gccaccggca aagtgaacgg caagggctcg ttcggcctgc tcggcatgcg
     1501 cgagcgcgcg ctgcgcctgg gcggcgagct gagcatcgac agcgcgcgcc ggcagggcac
     1561 ccgcatcacg gtgtcgctgc cgtgcggcga ggcgggcgca tgaaggtcct gatcgcggac
     1621 gaccatccgg tggtgcggcg cggcctgcgc cagatgctcg acgccgagag cgacctgagc
     1681 gtggtggtgg aggcgaagga cgggcacgag gtgatcgacc agtcggcgcg cgtgccctgg
     1741 gacgtggcgg tgctcgacta caacatgccg ggcaagggcg gcatcgaact ggtgaaggag
     1801 ctgcgccgcc gctatcccga ccggccggtg ctggtgctca gcatgtaccc ggaggaccgc
     1861 tacgcgctgc gcctgctgaa ggccggcgcg gcgggctacc tcaacaagga aagcgccgcc
     1921 gacgagctgg tcggcgcgct gcgcaaggtg gcgggcggcg ggcgctacgt cagcgcggcg
     1981 ctcggcgaga agctggcgca ggcgatggcc ggggacggcg acgcgccggc ctacgagaag
     2041 ctctcggacc gcgagtacca gatcatgtgg ctgatcgcca gcggcaagac ggtgggcgag
     2101 gtcgcgcggc agctgttcct cagcccgaac acggtcagca cctaccgctc gcgcatcttc
     2161 gcgaagctgg gggtgaagaa caattccgag ctcatgcgct atgccatccg ccaccagctg
     2221 gtggaatgaa atgggggaga tttcgatgca gaccatcgac aacggcttgc aggcggtcct
     2281 cgaccggggc gaactgctgc gcgcgctgcg cgcctaccgg cgcggcgact tcgcggtgcg
     2341 catgcccctc acgctgccgg gcatcgacgg cgacatcgcg caggcgttca acgaggtggt
     2401 ggagatgaac gcggcgctcg ccgccgagat ggcgcgcgtc agcgaccagg tcggcaagga
     2461 gggccgcgtc gggcagcgcg tcggcctgca cggggcgagc ggcgcctggg cgggctgcgt
     2521 cgactcggtc aacacgctga tcggggacgt cgcctacccg gtgaccgagg tggcgcgggt
     2581 catcggctcg gtggccaagg gcgacctgtc gcagaccatg gtgctcgagg tggacggccg
     2641 cgcgctgcgc ggcgatgtgc tgcgcatcgc cacggtggtc aacaccatgg tcggccagct
     2701 caacgccttc gcctcggagg tgacgcgggt ggcgcgcgag gtgggcaccg acgggaaact
     2761 gggcggccag gcgcaggtgc ccggcgtggc gggcgtatgg aaggacctca ccgacaacgt
     2821 gaactcgatg gcggccaacc tcaccggcca ggtgcgcaac atcgccgagg tgaccacggc
     2881 ggtggccaag ggcgacctgt cgaagaagat cacggtggac gtgaagggcg agatcctgga
     2941 gctgaagagc accatcaaca cgatggtgga ccagctgggg gccttcgcct cggaggtgac
     3001 gcgggtggcg cgcgaggtgg gcaccgacgg gaaactgggc ggc
//
LOCUS       DASRIE010000032         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2903316, whole genome shotgun sequence.
ACCESSION   DASRIE010000032 DASRIE010000000
VERSION     DASRIE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2903316"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFB53_01520"
     CDS             <1..903
                     /locus_tag="VFB53_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZN85439.1"
                     /translation="GNPDVDAIHSYTKLKHRGEGESWWSVLVHRYRMIAALRRAGFDY
                     VLLGKSTFDRHGLALARQVRPRHIVGIPPDAGQRAAGITDPVAPAPFAQGHEVEAIMK
                     LGAPLGVDTAPGPARVFASPARVAEWRGRFPALAQPGAKPWIALHISARHPRRLWAVE
                     RFIELARTLARDGAGIVLAWAPGGSDDPRHPGDDERAARIATGVGPGVPLIPAKTTTL
                     ADLIAVLSLSRGFIGADGGAMHIAAGLGLPVVALFEDLPDKKRHWHPWHVPYEIVSSG
                     PDDVAGVTVDQVAQAWARVSSRPK"
     gene            900..2147
                     /locus_tag="VFB53_01525"
     CDS             900..2147
                     /locus_tag="VFB53_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN85440.1"
                     /translation="MKPETLLAWVAALFLAACLFSHTVALRLLLLAAGIVLASITIAR
                     DRHGIKALPPIWLPFLLWGLWAAASIAWSVEPDRSEKEWRNEVFYTGAALWICFVGAQ
                     ARNAGRIVLPVLGITITAAIAIALWTYSRGWTEYLVGWHSGPGDHSSALLTLLPCLAM
                     AAWYGSRAGWARPVRVLMPLLAVLFAASAYFTLNRTLWLGFAVQLMLLGGLLLMRSDR
                     FQRARLTRQRILSACVALIAVISLAFFSIQATREATGTGKALQADPRLLLWPEVVEQI
                     AERPLVGYGFGRGLLREPLREDFGTLDSHLWHTHNIVLEALVQGGLSGLVLLALLLGM
                     LLREGWRYARSADERTMACGIALIGVVAGMVVRNMTDTLFVRQNSLFFWGMAGVLLGL
                     PLSGSATSTPAPSAAAARAPRPG"
     gene            complement(2080..>3228)
                     /locus_tag="VFB53_01530"
     CDS             complement(2080..>3228)
                     /locus_tag="VFB53_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZN85441.1"
                     /translation="RSLDVPVKTLPTLSDLVSGDADLARQLRPVEVEDVLGREPVEVD
                     FASIAEYLKGEVVLVTGAGGSIGSELCRQIARYAPSRLVLFDASEFALYSIEQEFRDR
                     FPRVAVAAVIGDAKDARRVGEVFARYQPQAVFHAAAYKHVPLMEDENAFEAVLNNVAS
                     TITVARAAQQAGATKFVLVSTDKAVNPVNVMGASKRLAEMVCQALEGNGTQSVVVRFG
                     NVLGSTGSVVPRFHSQIAAGGPVTVTHPDMERYFMSIPEAAQLVLQAGMMGKGGEIYV
                     LDMGEPVKIVELARQMIRLSGFDEDDIRIEFTGLRPGEKLYEEPLADAEKTLRTPHPK
                     LRVAQARSPDDGFLEALLAWLERPGEVSPDAVRAQLRRWVPEYSSPTR"
BASE COUNT          432 a         1213 c         1081 g          502 t
ORIGIN      
        1 ggaaatcccg acgtcgacgc gatccacagc tacaccaagc taaagcatcg tggggagggc
       61 gagtcctggt ggtcggtgct ggtccaccgc taccggatga tcgccgcgct gcgccgcgcg
      121 ggcttcgact acgtgctgct cggcaagtcc accttcgacc gccacggcct ggcgctcgcg
      181 cgccaggtgc gcccccggca catcgtcggc attccccccg acgcggggca gcgcgccgcc
      241 ggcatcaccg acccggtggc gccggcgccg ttcgcccagg ggcacgaagt cgaagccatc
      301 atgaagctcg gcgcgccgct cggcgtggac accgcgccgg gaccggcgcg cgtgttcgcc
      361 tcgcccgcgc gcgtggccga atggcgcggc cgcttcccgg cgctcgccca gcccggcgcc
      421 aagccctgga tcgcgctcca catcagcgcg cgccacccgc gccgcctctg ggcggtcgag
      481 cgcttcatcg agctcgcgcg caccctggcg cgcgacggcg ccggcatcgt gctcgcctgg
      541 gcgcccggcg gcagcgacga cccgcgccac cccggcgacg acgagcgcgc ggcgcggatc
      601 gcgaccggcg tcggccccgg cgtgccgctc atcccggcga agaccacgac cctcgccgac
      661 ctgatcgcgg tgctctcgct cagccgcggc ttcatcggcg ccgacggcgg cgcgatgcac
      721 atcgccgccg gcctcggcct gccggtggtc gcgctgttcg aggacctgcc cgacaagaag
      781 cgccactggc acccctggca cgtgccctat gaaatcgtgt cctcgggacc cgacgacgtc
      841 gccggcgtca cggtcgacca ggtggcgcag gcgtgggccc gcgtatcatc gcgcccgaag
      901 tgaaaccaga gaccttgctc gcgtgggtcg ccgcgctgtt cctcgccgcg tgcctgttca
      961 gccatacggt ggcgcttcgc ctcctcttgc tcgcggccgg catcgtgctc gccagcatca
     1021 ccatcgcgcg cgaccggcat ggcatcaaag cgctgccgcc gatctggctg ccgttcctgc
     1081 tctggggcct ctgggccgcg gcttcgatcg cgtggtcggt cgagcccgac cgcagcgaga
     1141 aggagtggcg caacgaggtg ttctacaccg gcgcagcgct gtggatctgc ttcgtgggcg
     1201 cgcaggcgcg caacgccggg agaatcgtgc tgccggtgct cggcatcacc atcaccgccg
     1261 ccatcgcgat cgcgctctgg acctactccc gcggctggac cgagtacctg gtgggctggc
     1321 acagcggccc cggcgatcat tccagcgcgc tgctcacctt gcttccctgc ctcgcgatgg
     1381 cggcgtggta cggcagccgc gccggctggg cacggccagt ccgtgtgctc atgccgctgc
     1441 tcgccgtgct cttcgccgcg agcgcctatt tcacgctgaa ccgcaccctg tggctcggct
     1501 tcgccgtcca actgatgctg ctgggcggcc tgctcctcat gcgctccgat cgctttcagc
     1561 gcgccaggct cacccggcag cgcatcctca gcgcctgcgt cgcgctgatc gcggtcattt
     1621 cgctcgcctt cttcagcatc caggccacgc gcgaggccac cggcacgggc aaggcgctgc
     1681 aggccgatcc ccggctgctg ctgtggcctg aagtcgtgga gcagatcgcc gagcggccgc
     1741 tggtcggcta cggcttcggc cgcgggctgc tgcgggaacc gctgcgcgag gatttcggga
     1801 cgctcgacag ccacctctgg cacacgcaca acatcgtgct cgaagccctg gtccagggcg
     1861 gcctgtccgg cctggtactg ctcgcgctcc tcctcgggat gctgctgcgc gaaggctggc
     1921 gctacgcgcg ctcggcggac gagcgcacca tggcctgtgg catcgcgctc atcggggtcg
     1981 tggcggggat ggtggtgcgc aacatgaccg acaccttgtt cgtgcgccag aactcgcttt
     2041 tcttctgggg aatggccggc gttctgctcg gcctgccgct cagcgggtcg gcgacgagta
     2101 ctccggcacc cagcgccgca gctgcgcgcg caccgcgtcc gggctgacct cgcccggcct
     2161 ttccagccag gcgagcagcg cctccaggaa gccgtcgtcg ggcgagcgcg cctgcgcaac
     2221 gcgcagcttc gggtgcggcg tgcgcagcgt cttttccgcg tcggcgagcg gctcctcgta
     2281 cagcttctcg cccgggcgca gcccggtgaa ctcgatgcgg atgtcgtcct cgtcgaagcc
     2341 cgagagccgg atcatctggc gcgcgagctc gacgatcttc accggctcgc ccatgtcgag
     2401 cacgtagatc tcgccgccct tgcccatcat ccccgcctgc agcacgagct gcgccgcctc
     2461 cgggatggac atgaagtagc gctccatgtc cgggtgcgtc acggtcaccg gcccgccggc
     2521 ggcgatctgg ctatggaagc gcggcaccac gctgccggtg gagccgagca cgttgccgaa
     2581 acgcaccacg acggattggg tgccgtttcc ctccagcgcc tggcagacca tctccgcgag
     2641 gcgtttcgaa gcgcccatca cgttgaccgg gttcaccgcc ttgtcggtgg acaccagcac
     2701 gaacttcgtt gccccggcct gctgcgccgc gcgcgccacg gtgatggtgc tcgcgacgtt
     2761 gttcagcacc gcctcgaatg cgttttcgtc ctccatgagc ggcacgtgct tgtaggccgc
     2821 cgcatggaac acggcctgcg gctgatagcg cgcgaacacc tcgcccacgc gccgcgcatc
     2881 cttggcatcg ccgatgaccg cggccaccgc gacgcgcggg aagcggtcgc ggaattcctg
     2941 ctcgatcgag tacagcgcga actccgacgc gtcgaacagc accagccgcg aaggcgcata
     3001 gcgcgcgatc tgccggcaga gctccgagcc gatcgagccg ccggcgcccg tcacgagcac
     3061 gacctcgccc ttcaggtact cggctatcga cgcgaagtcg acctcgaccg gctcgcggcc
     3121 gagaacgtcc tcgacctcga ccggccgcag ctggcgggca aggtccgcgt cgcccgagac
     3181 gaggtcggag agcgtcggca gggtcttgac cggaacgtcg agcgaccg
//
LOCUS       DASRIE010000033         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_34042, whole genome shotgun sequence.
ACCESSION   DASRIE010000033 DASRIE010000000
VERSION     DASRIE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34042"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /gene="metX"
                     /locus_tag="VFB53_01535"
     CDS             <1..137
                     /gene="metX"
                     /locus_tag="VFB53_01535"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464867.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZN85442.1"
                     /translation="RALLDNRRIVSYLELDAPGGHDAFLLEDPRYHGALRSYFANIEL
                     "
     gene            134..718
                     /gene="metW"
                     /locus_tag="VFB53_01540"
     CDS             134..718
                     /gene="metW"
                     /locus_tag="VFB53_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZN85443.1"
                     /translation="MRPDQEAIIRWVPEGARVLDLGCGDGELLRSLWEQRKAPGYGVE
                     IEDQAVLACVENDVNVLQVDLESGLSLFADGSFDCVILSETLQTIHRTEFLLREMLRV
                     GREGIVSFPNFGHWSARLQIALGRMPVSETLPYQWYETPNVHHCTISDFEDLCRRVGI
                     RIRERLVLHEARPVTWMPNLLGSLAVYRCTNGRP"
     gene            718..1239
                     /locus_tag="VFB53_01545"
     CDS             718..1239
                     /locus_tag="VFB53_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85444.1"
                     /translation="MPQVLFVCTGNVFRSLTAEYALRAALNGRAGIVVGSAGTEDYAH
                     TVMPEIREYLLGKGLDVSAHRRRTLTQAMVESAHLVVAMSVDHREFIRQRFGRSAPLF
                     LECCGGAPEALLDLHEAVRDYRTNRAAALAYLHETMDRIVELTPRLAQRLALLAGGAD
                     RDVGEAGADHQPE"
     gene            complement(1171..>2238)
                     /locus_tag="VFB53_01550"
     CDS             complement(1171..>2238)
                     /locus_tag="VFB53_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85445.1"
                     /translation="VRLPLIGRFGQRRSWLWLTGALVMAAVAFLGAADPAGALYLVAL
                     AAIAVGIAGATFDIIIDAYRIELLEPRQLGAGSGMSQYGWRIGAAAAGALALVLAHRI
                     GWTAAYMACAAFALPAMIVGLVMGEPARHREPARPHGVAEAVVAYFSPLTEFLRRQGA
                     LVVLLFVLIHKIGDTLANLTLRLLFQDLGFSNDEVAFYDVGIGFAALLVGVFVGGILY
                     TRLGMKRSVLVSLVLMAVSNLSFAGLAAAGHTNIGMAAAVGFENFASGIGGVTVVAYL
                     SALCNLRFTATQYALLSALASIAGRFLTGTSAGSLINATGYVNFYLLTTLIALPGVLL
                     FWLMIRSGLADVSIGSAGKES"
BASE COUNT          366 a          776 c          798 g          298 t
ORIGIN      
        1 tacgcgccct gctcgacaac cggcgcatcg tctcgtatct cgagctcgac gcgcccggcg
       61 gccacgacgc gttcctgctg gaagacccgc gctaccacgg cgcgctgcgc tcctacttcg
      121 ccaacatcga gctatgaggc cggaccagga ggccatcatc cgctgggtac ccgagggcgc
      181 gcgcgtgctc gacctgggct gcggcgacgg cgagctgctg cgctcgctct gggagcagcg
      241 caaggcgccg ggctacggcg tggagatcga ggaccaggcg gtgctcgcgt gcgtcgagaa
      301 tgacgtcaac gtgctgcagg tggacctgga aagcgggctc tcgctgttcg ccgacggctc
      361 cttcgactgc gtgatcctgt ccgagacgct gcagaccatc caccgcaccg agttcctgct
      421 gcgcgagatg ctgcgcgtgg gccgcgaagg catcgtcagc tttccgaact tcggccactg
      481 gagcgcgcgc ctgcagatcg cgctcgggcg catgccggtc tcggagacgc tcccctacca
      541 gtggtacgag accccgaacg tgcaccactg caccatcagc gacttcgagg atctctgccg
      601 gcgcgtcggc atccgcatcc gcgagcgcct ggtgctgcac gaggcgagac ccgtcacctg
      661 gatgcccaac ctgctcggca gcctcgcggt ctatcgctgc accaacggcc gcccctgatg
      721 ccgcaggtgc tgttcgtgtg caccgggaac gtcttccgca gcctgaccgc ggagtacgcg
      781 ctgcgcgcgg cgctgaacgg ccgcgccggc atcgtggtgg ggtcggcggg aaccgaggac
      841 tacgcgcaca cggtgatgcc ggagatacgg gaatacctgc tggggaaggg gctcgacgtc
      901 agcgcccacc ggcgccgcac gctcacccaa gcgatggtcg agagcgcgca cctcgtggtc
      961 gcgatgagcg tcgaccaccg cgagttcatc cgccagcgct tcggccgcag cgcgccgctc
     1021 ttcctggaat gctgcggcgg cgcgccggag gcgctgctcg acctgcacga ggcggtgcgc
     1081 gattaccgca ccaaccgggc cgccgccctc gcctacctcc acgagacgat ggatcgcatc
     1141 gtcgagctca cgccgcggct cgcgcagcgg ttagctctcc ttgccggcgg agccgatcga
     1201 gacgtcggcg aggccggagc ggatcatcag ccagaatagg agcacgcccg gcagcgcgat
     1261 cagcgtggtg agcaggtaga agttgacgta gccggtggcg ttgatgagcg agccggcgct
     1321 ggtgccggtg aggaagcgcc cggcgatgct tgcgagcgcc gagagcagcg cgtactgggt
     1381 cgcggtgaag cgcaggttgc agagcgccga gaggtacgcg accaccgtga cgccgccgat
     1441 gccgctcgcg aagttctcga agccgaccgc cgcggccatg ccgatgttgg tatggccggc
     1501 ggcggcgagg ccggcgaagc tcaggttcga aaccgccatc agcaccaggc tcaccagcac
     1561 cgagcgcttc atgcccaggc gcgtgtagag gatgccgccg acgaacacgc ccaccagcag
     1621 ggcggcgaag ccgatgccga cgtcgtagaa cgccacctcg tcgttgctga agccgaggtc
     1681 ctggaacagc agccgcagcg tgaggttggc gagcgtgtcg ccgatcttgt ggatcagcac
     1741 gaagagcagc accaccagcg cgccctgccg ccgcaggaac tcggtgagcg ggctgaagta
     1801 ggccacgacc gcttcggcca cgccgtgcgg cctggcgggc tcgcggtgcc gcgcgggctc
     1861 gcccatcacc aggccgacga tcatcgccgg cagcgcgaac gccgcgcacg ccatgtaggc
     1921 cgcggtccag ccgatgcggt gcgcgagcac gagcgcgagc gcgcccgcgg cggcggcgcc
     1981 gatgcgccag ccatattgcg acatgccgga gccggcgccg agctggcgcg gctcgagcag
     2041 ctcgatgcga taggcgtcga tgatgatgtc gaaggtcgcg cccgcaatgc cgaccgcgat
     2101 ggcggcgagc gcgaccaggt agagcgcgcc ggccgggtcg gcggcgccga ggaacgcgac
     2161 cgcggccatg accagcgcgc cggtaagcca cagccagctg cggcgctggc cgaagcgacc
     2221 gatgagcggc aggcgcac
//
LOCUS       DASRIE010000034         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_34218, whole genome shotgun sequence.
ACCESSION   DASRIE010000034 DASRIE010000000
VERSION     DASRIE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34218"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFB53_01555"
     CDS             <1..608
                     /locus_tag="VFB53_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HZN85446.1"
                     /translation="ELRRARQLRPFVDAADHLLDLHSMHEPCRPIMVCAKSEKSVALS
                     RRIGMPADLLLDTGHPAGLRMIERGAFNDPASPRSAVLIECGQHWEKSSVQVAIDTAL
                     RFLKATGAVEGAWADARLKLAPPPEQRVIRVTEPVVAQTMQFRFARPWKGLEVLPKKG
                     TLVASDGDREWRTPYDDCVLVMPSLAHLKPGTTLVRLGRYA"
     gene            complement(615..1208)
                     /gene="msrA"
                     /locus_tag="VFB53_01560"
     CDS             complement(615..1208)
                     /gene="msrA"
                     /locus_tag="VFB53_01560"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZN85447.1"
                     /translation="MTRLMLVLALLLLPAAAQAQSLAKATFAAGCFWCTEEVFDKLPG
                     VTATVSGYMGGRTKNPTYEQVSAGATGHTEVLQVTYDPARLSYEKLLEAFWVNHDPTT
                     INSQFCDHGSQYRPGIFTHTPEQQRLAEASKAKWEKDKPFKDKILTPIAAATEFWPAE
                     DYHQDYYLKNPVRYRFYSTGCGRYSRLDELWGKYRAK"
     gene            complement(1262..2098)
                     /locus_tag="VFB53_01565"
     CDS             complement(1262..2098)
                     /locus_tag="VFB53_01565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN85448.1"
                     /translation="MRTRFLAVAAAAALALPAFGQLPDKPAAKPAAKPAAKPAASGPV
                     ATVNGTAIPRTRLEYMLRQQVARGAPDNEQTRAAVREDLINRELVLQEAARAGTAKKQ
                     EVQLQLDMARQEVMVGAYLNDYMQKHAITDGDVQKEYDRAREQTGATEYRARHILLET
                     EEEANRVLADLKKGGKFEELARKSSKDEGTRERGGDLDWNVPAAFDKAFSDAMVKLDK
                     GQVTPQPVRSRFGFHVIQLEDQRSVNFPPLAQVRPQIQQRLARQKVETLVKGLRAKAK
                     VE"
     gene            complement(2102..2371)
                     /locus_tag="VFB53_01570"
     CDS             complement(2102..2371)
                     /locus_tag="VFB53_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZN85449.1"
                     /translation="MNVAQDIRRRLAALQPLELELVDESDAHRGHAGHREGGGTHWRL
                     TIVSPAFAGQSTVARHRLVYRALGELMQHPIHALAISARAPEEKG"
     gene            complement(2368..2898)
                     /locus_tag="VFB53_01575"
     CDS             complement(2368..2898)
                     /locus_tag="VFB53_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HZN85450.1"
                     /translation="MKFLFDLFPVILFFAAFQLWDIYVATAVAIAASFAQIGWLVLRR
                     RKIDPMLWASLCIIVVFGGLTLALRDKTFIQWKPTVLYWLFGAVLAGGALSGRNLIRA
                     MMSQQMRLPDPVWARLNWSWVAFFAFMGAANLYVAYNFSESAWVSFKLFGGMGLMLAF
                     VLAQAAFLARYVEEKP"
     gene            complement(2934..3617)
                     /locus_tag="VFB53_01580"
     CDS             complement(2934..3617)
                     /locus_tag="VFB53_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85451.1"
                     /translation="MPAWIAALSPDTVMIAIAGVGGVIALSLLIEAVRRLRDWQIRTG
                     IVFLVIAAAVAGLGTALALLAASLHTYSRLTAEQEAARAVFRELAPKRYELILVLRNE
                     PARRFEIRGDEWQIDARVLKWKGLATTLGLDTVYRFERVAGRYADTGQEKTAPRTVHA
                     LSGDAGFDLWSLLKEQQRWLPLADALYGSAAYAPMGDGVQYAVNVSATGLLLRPLNEA
                     AKKALGGWK"
     gene            complement(3621..4073)
                     /locus_tag="VFB53_01585"
     CDS             complement(3621..4073)
                     /locus_tag="VFB53_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZN85452.1"
                     /translation="MLLKGSCHCGAVRFSVQSETPHPYMSCYCSICRKTAGGGGYAIN
                     IMGRAESLEVKGRSRSYRATRGGGERHFCPRCGSALWVWDRRWAEWVYPFASAIDSKL
                     PAPRERQHIMLDSKASWVPVPKGRAEKRFREYPRESILDWHRKRGLLA"
     gene            complement(4093..4449)
                     /locus_tag="VFB53_01590"
     CDS             complement(4093..4449)
                     /locus_tag="VFB53_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85453.1"
                     /translation="MGPLRGSAALALLAAAFAAQACAVPGDGTPLRHALLKVKYLAET
                     EAWERAAMQAAPVQYVLSLDAPLRHRGRCYWPVEARSGGALWQRFYATAGGERLLVQG
                     TQGKLHTLDEWRKASR"
     gene            complement(4419..5003)
                     /locus_tag="VFB53_01595"
     CDS             complement(4419..5003)
                     /locus_tag="VFB53_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HZN85454.1"
                     /translation="MSNLARLRSFIHDMTRLADRHGEDEQRMLDEGEKLLRALIAQDD
                     WLPEEFARPSAEGYRQYLLHCDPMERFSVVSFVWLPGQRTPIHDHTVWGLVGVMRGEE
                     ICEEYSKQLKATGRHPVLRGQVDRVSPRIGDIHVVSNESRDVAVSIHVYGANIGAVRR
                     HTFDPATGSPRDFVSGYANATIPNLWDRSGEARR"
     gene            5022..>5851
                     /locus_tag="VFB53_01600"
     CDS             5022..>5851
                     /locus_tag="VFB53_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN85455.1"
                     /translation="MSAILSPRLAIPLLMLLASTFAANHIAARVAFDHGASVAAAVTV
                     RSGVTALAIVALLRLQGVSLALPRATLARGLGIGLLVAVQSYCLYSSVAVIPVALALL
                     AFNTFPLLYVLLSWAAGGENPGRRALVAMPLALVGLVLALDIVGSLHAIAGRWAEIGL
                     GVGWALGSATSFACVLFLTTRHLQAVDGRVRTLLTMGTTAVVVGLAGAATGSLALPAD
                     ATGWFGLALLTVLYGSAITAIFTVVPRLKSPSNTAALNFEPIAALILGWAILGQAVAP
                     R"
BASE COUNT          796 a         2073 c         2045 g          937 t
ORIGIN      
        1 ccgaactgcg ccgcgcgcgc cagctgcgcc ccttcgtcga cgcggccgac cacctgctcg
       61 acctgcactc catgcacgag ccctgccgcc cgatcatggt gtgcgccaag agcgagaaga
      121 gcgtcgcgct gtcgcggcgc atcggcatgc cggccgacct gctgctcgac accgggcatc
      181 cggcgggcct gcgcatgatc gagcgcggcg ccttcaacga cccggcgagc ccgcgcagcg
      241 ccgtgctcat cgagtgcggc cagcactggg agaagagctc ggtgcaggtg gcgatcgaca
      301 ccgcgctgcg cttcctcaag gccaccggcg cggtggaagg cgcctgggcc gacgcgcggc
      361 tcaagctcgc gccgccgccc gagcagcggg tcatccgggt gaccgagccc gtggtggcgc
      421 agaccatgca gttccgtttt gcgcgcccct ggaaggggct cgaggtcctg ccgaagaagg
      481 gcacgctggt ggcgagcgac ggcgaccggg aatggcgcac gccctacgac gattgcgtgc
      541 tggtgatgcc ttcgctggcg cacctcaagc cggggaccac cctggtgcgg ctcggccgct
      601 atgcctagcg cgcgctactt ggcgcggtac ttcccccata gctcgtccag gcgcgagtag
      661 cgcccgcagc cggtggaata gaaccggtag cgcaccgggt tcttcaggta gtagtcctgg
      721 tggtagtcct ccgccggcca gaactcggtg gccgccgcga tcggcgtgag gatcttgtcc
      781 ttgaagggct tgtccttctc ccacttcgcc ttcgaggcct cggcgaggcg ctgctgctcc
      841 ggcgtatggg tgaagatgcc cggccggtac tgggagccgt ggtcgcagaa ctggctgttg
      901 atggtcgtcg ggtcgtggtt gacccagaac gcctccagca gcttttcgta gctcagcctg
      961 gccgggtcgt aggtgacctg cagcacctcg gtgtgtccgg tggcgccggc ggacacctgc
     1021 tcgtaggtcg gattcttcgt cctgccgccc atgtagcccg acacggtcgc cgtgaccccc
     1081 ggcagcttgt cgaagacctc ctcggtgcac cagaagcagc ccgccgcgaa cgtggccttg
     1141 gcgagggatt gcgcctgcgc cgccgccggc agcagcagca aggcgaggac cagcatcagt
     1201 cgtgtcatgg ctcgtgtgcc tcctgcggtg ttggtcgcgc cgggccggcg ttccttaccg
     1261 cctactcgac tttcgccttg gcgcgcaggc ccttcaccag ggtttccacc ttctggcgcg
     1321 ccaggcgctg ctggatctgc ggccgcacct gcgccagcgg cgggaaattc accgaccgct
     1381 ggtcctccag ctggatgacg tggaagccga agcgcgagcg caccggctgg ggcgtgacct
     1441 ggcccttgtc cagcttgacc atggcgtcgg agaacgcctt gtcgaaggcc gccggcacgt
     1501 tccagtcgag atcgccgccg cgctcgcgcg tgccctcgtc cttggaggat ttgcgcgcca
     1561 gctcctcgaa cttgccgccc ttcttcaggt cggccagcac ccggttcgcc tcttcctcgg
     1621 tctcgagcag gatgtggcgc gcgcggtact cggtggcgcc ggtctgctcg cgcgcccggt
     1681 cgtactcctt ctgcacgtcg ccgtcggtga tcgcgtgctt ctgcatgtag tcgttgaggt
     1741 aggcgccgac catcacctcc tggcgcgcca tgtcgagctg cagctgcacc tcctgcttct
     1801 tcgcggtgcc ggcgcgcgcg gcctcctgca ggaccagttc gcggttgatc aggtcctcgc
     1861 gcaccgcggc gcgcgtctgc tcgttgtccg gcgcgccgcg cgcgacctgc tggcgcagca
     1921 tgtactcgag gcgcgtgcgc gggatggcgg tgccgttgac cgtggcgacc gggcccgagg
     1981 cggcaggttt ggccgccggc ttggcggcgg gcttcgcggc cggcttgtcc ggcagctgcc
     2041 cgaaggcggg cagggcgagc gcggcggccg ccgcaacggc gaggaaacgt gttctcatgg
     2101 atcagccttt ctcttctggc gcgcgcgccg aaatggcgag cgcgtggatc gggtgctgca
     2161 tcagctcgcc gagcgcccgg tacaccaggc ggtggcgcgc gacggtcgac tggccggcga
     2221 aggccggcga gacgatggtg agccgccagt gcgtgccgcc gccttcgcgg tggccggcgt
     2281 gcccgcggtg agcgtcgctt tcgtccacca gctccagctc cagcggctgc agcgcggcaa
     2341 ggcgccggcg gatatcctgc gcgacgttca tggcttctcc tcgacgtagc gcgcgaggaa
     2401 agccgcctgc gccagcacga aggcgagcat cagccccatg ccgccgaaca gcttgaagct
     2461 cacccaggcg ctttccgaga aattgtaggc cacgtacaga ttggccgcgc ccatgaaggc
     2521 gaagaaggcc acccagctcc aattgagccg cgcccatacc gggtcgggca ggcgcatctg
     2581 ctgggacatc atggcgcgga tcaggttccg tcccgagagc gcgccgccgg cgagcaccgc
     2641 gccgaacagc cagtacagca ccgtcggttt ccactggatg aaagtcttgt cgcgcagggc
     2701 gagcgtcagc ccgccgaata ccacgatgat gcaaaggctc gcccacagca tcggatcgat
     2761 cttccggcgg cgcagaacca gccagccgat ctgcgcgaag ctcgccgcga tggccactgc
     2821 ggtggcgaca tagatgtccc acagctggaa cgcggcgaag aacaggatca ccgggaagag
     2881 gtcgaacagg aacttcatgg cagcgcggcg attctaccgc agcgcggcgg cgcctacttc
     2941 cagccgccga gcgccttctt cgccgcctcg ttgagcgggc gcagcagcag gccggtggcg
     3001 gacacgttca ccgcgtactg cacgccgtct cccatcggcg cgtaggcggc gctgccgtac
     3061 agggcgtcgg cgagcggcag ccagcgctgc tgctccttga gcagcgacca aagatcgaag
     3121 ccggcgtcgc cggacagcgc gtgcaccgtg cgcggcgcgg tcttttcctg cccggtgtcg
     3181 gcgtagcgcc ccgcgacgcg ctcgaagcgg tataccgtgt ccaggccgag cgtggtcgcc
     3241 aggcccttcc acttcagcac gcgcgcgtcg atctgccact cgtcgccgcg gatctcgaag
     3301 cgccgcgcgg gctcgttcct gaggaccagg atcagctcgt agcgcttcgg ggcgagctcg
     3361 cggaacacgg cgcgcgccgc ttcctgctcc gcggtcagcc gggagtaggt gtgcaggctg
     3421 gcggcgagca gcgccagcgc ggtgcccagg ccggccaccg ccgcggcgat gacgaggaac
     3481 acgatgccgg tgcggatctg ccagtcgcgc aaccggcgca cggcttcgat cagcagcgac
     3541 agcgcgatga cgccgcccac gccggcgatt gcgatcatca ccgtgtcggg gctcagcgcg
     3601 gcgatccagg cgggcatggc tcaggcgagc aggccgcgct tgcggtgcca gtcgaggatg
     3661 gactcgcggg gatactcgcg gaagcgcttc tcggcgcgcc ccttgggcac cggcacccag
     3721 ctcgccttcg aatcgagcat gatgtgctgg cgctcgcgcg gtgcgggaag cttcgagtcg
     3781 atggccgagg cgaagggata gacccattcg gcccagcgcc ggtcccagac ccacagcgcg
     3841 ctgccgcacc gcgggcagaa atggcgctcg ccgccgccgc gcgtcgcccg gtaggacctg
     3901 ctcctgccct ttacctcgag cgactccgcg cggcccatga tgttgatggc gtagccgccg
     3961 ccgccggcgg tcttgcggca gatcgagcag tagcaggaca tgtaggggtg gggcgtctcc
     4021 gactgcacgg agaagcgcac ggcgccgcaa tggcaggagc ccttgagcag catgcgtcga
     4081 atataccgga ctctagcgcg aggctttgcg ccattcgtcc agggtgtgca gtttgccctg
     4141 cgtgccctgc accagcagcc gctcgccccc ggcggtggca tagaagcgct gccagagcgc
     4201 gccgccgctg cgcgcctcga ccggccagta gcagcggccc cgatggcgca gcggcgcgtc
     4261 cagcgacagg acgtactgca cgggggcggc ctgcatcgcc gcgcgctccc aggcctcggt
     4321 ctcggcgagg tatttgacct tgagcagcgc gtgccgcaac ggcgtgccgt cgccgggcac
     4381 cgcgcaggcc tgcgcggcaa aggccgcggc cagcagggct agcgccgcgc ttccccggag
     4441 cggtcccaca ggttcgggat ggtggcgttg gcatagccgg agacgaagtc gcgcggcgat
     4501 ccggtcgccg gatcgaaggt gtggcgccgc accgcgccga tgttcgcgcc gtagacgtgg
     4561 atgctgaccg ccacgtcccg gctctcgttg gacaccacgt gaatgtcgcc gatgcgcggc
     4621 gagacgcggt ccacctgccc gcgcagcacc ggatgccgcc ccgtggcttt cagctgcttc
     4681 gaatattcct cgcagatctc ctcgccgcgc atcacgccca ccaggcccca cacggtgtgg
     4741 tcgtggatcg gcgtcctctg gcccggcagc cacacgaagc tcaccaccga gaagcgctcc
     4801 atcgggtcgc agtgcaggag gtactgccgg tagccctcgg cggagggccg cgcgaactcc
     4861 tcgggcagcc agtcgtcctg cgcgatcagc gcgcgcagaa gtttctcgcc ctcgtcgagc
     4921 atgcgctgct cgtcctcgcc gtggcggtcg gcgaggcgcg tcatgtcgtg gatgaaactg
     4981 cgcaggcgcg cgaggttgct catgccttaa gcttcgggcc catgagtgcg attctatcgc
     5041 cccgcctggc cattccgctg ctgatgctgc tcgccagcac cttcgcggcc aaccatatcg
     5101 ccgcccgcgt ggccttcgac cacggggcca gcgtcgccgc ggcggtgacg gtgcgctcgg
     5161 gtgtcaccgc gctcgccatc gtcgcgctgc tgcgcctgca gggcgtgtcg ctggcgctgc
     5221 cgcgcgcgac cctggcgcgc gggctgggca tcggcctgct ggtggcggtg cagagctact
     5281 gcctgtattc gtccgtcgcc gtcatcccgg tggcgctggc gctgctcgcc ttcaacacct
     5341 tcccgctgct ctacgtcctg ctcagctggg cggccggcgg cgagaatccc gggcgccgcg
     5401 cgctggtggc gatgccgctc gcgctcgtcg gcctggtgct ggcgctggac atcgtcggct
     5461 cgctgcacgc catcgccggg cgctgggccg agatcggcct cggcgtgggc tgggcgctgg
     5521 gctcggcgac ctccttcgcc tgcgtgctgt tcctcaccac caggcacctg caggccgtgg
     5581 acgggcgggt gcgcacgctg ctcaccatgg gcaccaccgc tgtggtggtg ggcctcgccg
     5641 gcgccgccac cggctcgctc gcgctgccgg cggacgccac cggctggttc ggcctcgccc
     5701 tgctgacggt gctctacggc agcgccatca ccgcgatctt caccgtggtg ccgcggctga
     5761 agtcgcccag caacaccgcg gcgctcaatt tcgagcccat tgccgcgctg atcctgggct
     5821 gggcgatcct cggccaggcg gtggccccgc g
//
LOCUS       DASRIE010000035        13803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1089422, whole genome shotgun sequence.
ACCESSION   DASRIE010000035 DASRIE010000000
VERSION     DASRIE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089422"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1394
                     /gene="serS"
                     /locus_tag="VFB53_01605"
     CDS             117..1394
                     /gene="serS"
                     /locus_tag="VFB53_01605"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZN85456.1"
                     /translation="MIDIQLLRNSLASVVERLKTRGFVLEPAAFLALETRRKDIQTRT
                     ESLQARRNTLSKEVGIAKAKKDPAGADALMKQAAAAGDELKALEAENQLVQDQLRNFV
                     ALIPNLPHESVPVGASSEQNVEVRRRGTPRKFDFPAKDHVDVGTPLGLDFEAGAKLAG
                     ARFAVMKGQLARLHRALAQFMLDVHTTEHGYTEAYTPYMVSGACADGVSSLAKFKGDL
                     FKLEDRDLYLIPTAEYSVTNFVRDEILPLESLPLKYVCHSPCFRSEAGAAGKDTRGMI
                     RNHQFDKVELVQIAHPSQSYALHEELTAHAEAILQQLGLPYRVVTLCTGDTGFSAAKT
                     YDLEVWLPGQNAYREISSCSNFEAFQARRMQARFRNAQGKPEPVHTLNGSGLAVGRTL
                     VAILENFQNADGSVSIPAALRSRMGGAEKLARP"
     gene            1412..1501
                     /locus_tag="VFB53_01610"
     tRNA            1412..1501
                     /locus_tag="VFB53_01610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1446..1448,aa:Ser,seq:cga)
     gene            1526..2302
                     /locus_tag="VFB53_01615"
     CDS             1526..2302
                     /locus_tag="VFB53_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85457.1"
                     /translation="MIGVYRDIEGSMRLAGHVLRRFWVLKLPPVAEELVRQIYFTGAS
                     ATTTIVIRGALIGTLIIAYVIEVLNADAALAVKLLLLVVLREIGPLIAAVLVIQRSGT
                     AVATELALMKISGEVASLRHLRIDPQDYLVVPRVAGIALANTALTVYVQFIAVGGGMV
                     LAALVIDASLEELAANFFLLASPLDVVYSVAKSLLFGAAIGVIACYYGLNPPGNSVNA
                     VPKAAISAVTQATLAVMLLNVVFAYLVYGSALFGLFTADQ"
     gene            2299..2925
                     /locus_tag="VFB53_01620"
     CDS             2299..2925
                     /locus_tag="VFB53_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85458.1"
                     /translation="MSAAAEVALGYEPQAGSGLPGERIWIEAGGSHVVPAVGAALKPV
                     LLAAVIELRPRKDARVVVLGAETGSLPEKARAALRAQVAFLPAGGGLLSNLNAWENIT
                     LPIGYHEPKRARGLASQVYALLEQFGADARALLAKLPESMTPDERMLAGYVRMRLGRP
                     RLVLGEDPGGAPGASERRRAAAFAAAYLADCPEGTFVQLQEDASDGSG"
     gene            2951..3922
                     /locus_tag="VFB53_01625"
     CDS             2951..3922
                     /locus_tag="VFB53_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN85459.1"
                     /translation="MQEQQALPRRFPGIRRRISLFLVTALVGLVGAVLLVAYKQGAFV
                     RQTSIFFYAEDVIGINKGMTVRLFGLPVGSVKHLEITTRGVQVELSIVSEYVPRLTKG
                     TNARLTREGYIGAASLLLLPGGEAKDARAPLAAGDVIGYLPNRGVAELVDDIKNQLAP
                     VVGELRGMMAELNRPEGDFRKSLQGASVVLQQLPETNRELRQLLRDTDRAVTAVGGRA
                     EAALVATERAGTRLERELPELSRQAGATLQAVAEAATEIRSTTRKNGEALHQALVQTP
                     ALVRDGAQLVRDGQDVVGAAKNSWLIRDYVEPAAMRTLPLDSFEAQR"
     gene            3922..4791
                     /locus_tag="VFB53_01630"
     CDS             3922..4791
                     /locus_tag="VFB53_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85460.1"
                     /translation="MRLTAAAACALLAACAARTPAPPAPTQATAVQPTAAMEANRRAE
                     ASLRRGELDGAAFHYREALRLSLAVEDAEGIAANAVNLSIVHQRQGKTEEARASLAPL
                     LERATLRHAPVHRAQAALRRAVLDLDERRHASAAEWAARATDWCGTPCALAGPIRNVQ
                     GQLALAAGRLEEAAAAAANALAESRAAGQAAEIANAHRLRGMTALAAGDGAAALAALG
                     EALAIDRELGIPRRIFLDLVGLGGASKLQGDRQAARGYYARAQAVSEADRDAAGSAEA
                     RALLDELDLASRK"
     gene            4788..7103
                     /locus_tag="VFB53_01635"
     CDS             4788..7103
                     /locus_tag="VFB53_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN85461.1"
                     /translation="MNAPAVQFDPAAARFGSARTQKRLEDDRLLVGKGLYSDDRRFEG
                     EAALVLVRSPYAHAKIRSIDLAAVRAAPGVLAAWTMAELAADGVQHIPTPPLFKRADG
                     QPMQAPLRTPLAGERVLYVGHPLVAVVAQTRAQAQDAAELAAIDFEELPCVADARAAV
                     KPGAPQLAPGAPGNVAAETRFGKPDEARAAFARAAHVTEIELHNQRVIAMALEPRCAI
                     GVHENGRTTVYTQSQQPTGARDGLAAALGAKPADLRIVNGDIGGGFGMKTGATPEEAL
                     VAYAAKKLGRPVRWRGDRSEEFLAAHMGRDMHFKAALALDRDGRILALRLHSLGNMGA
                     VPVNSSAMIPLMMVAKVATSVYRVPAVDYHFQGVLTNTMATGAYRGAGRPEGNFVIER
                     LVEKAAREMRIDPVEMRRRNLLQPGDFPVATHAGEVYDTGNFPGILDGLVELADWKGF
                     PARAAESKRRGRLRGRGLATYLEWTGALPTETVDIEVDAAGRVTVFSGTQAMGQGLET
                     SYVQLVTEVLQIPAARVTIVQGDTDRANGVGSVGSRSAFVGGSAVVAAGHTLVRRGRE
                     LAADALEAAAQDIEFRDGRFTIAGTDKSLDLAGLAARQAAKILRVSATQTPSTPSWPN
                     GAQACEVEIDPETGEVALARLTSMDDIGRIINRMIVEGQIHGGMAQGVGQALYEQAVY
                     DESGQLLTGSLMDYCVPRADQFPPLAAAFDESVPCTTNLLGVKGCGEIGTIGAVPAVV
                     HAVLDALAEFGVVHLEMPLTPEKVWRAMRRA"
     gene            7109..7807
                     /locus_tag="VFB53_01640"
     CDS             7109..7807
                     /locus_tag="VFB53_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZN85462.1"
                     /translation="MRGGADRAFALALLASILLHALLLGALPSWRAPPEPPAALPLTA
                     RLAEAPAAPASAPPAPVSVEEDARPAPPAAARAPLRRPAPSPARAALPVQPALSERAL
                     APPAPLAPAAPGPVARIDPLPAAPPPAESDALATYRANVNAAATRFKRYPRSAIDNNW
                     EGEVVVLMSIGADGRIASLRVRTSSGYEVLDRQALDMFRNAKPFVPLPRELRGQEFEV
                     ELRAIYSLKDQGSG"
     gene            complement(7792..8823)
                     /locus_tag="VFB53_01645"
     CDS             complement(7792..8823)
                     /locus_tag="VFB53_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85463.1"
                     /translation="MNQLEVQGLHHGYNGAEVVRGLDFALPRGAIGCLLGPSGCGKTT
                     VLRCIAGFEAIRAGSIRLNGVLVSGPGSMLPPERRHVGMVFQDYALFPHLSVERNIAF
                     ALRALPAAARAARVRELADLTGLAAVLASYPHELSGGQQQRVALARALAPRPELLLLD
                     EPFSNLDVDLRERLSLEVRDIIKASGATAVLVTHDQHEAFAVADEIGVMHEGRIVQWD
                     SAYNLYHRPASRFVADFVGQGVFLPARRLGARQVEIELGVIEGEIPGGAAEILLRPDD
                     VVHDDASALKAEVLHKAFRGAEILYTLRLASGRKVLALVPSHHNHALGERIGIRLDVD
                     HVVAFQPEP"
     gene            8876..10510
                     /locus_tag="VFB53_01650"
     CDS             8876..10510
                     /locus_tag="VFB53_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZN85464.1"
                     /translation="MLFYQTVRRGGSGLSLAIAALLALPILWVASSVLQPAGDAWRHI
                     AATLLPLYAANTALLLALVACGVISIGVLSAWLVASTRFPGVRLLEWALFLPLAMPAY
                     VMAYAYTDWLQFTGPVQEALRSLTGWQAREYWFPEIRSLGGAAAVLSLALYPYVYLIA
                     RAAFRELPRSAIEAGRLAGHSAGRNFLRVALPLARPAIAAGTALALMETLADFGTVSY
                     FALEVFTTGIFKAWLSMGDSLAAAQLSSCLLAFVLLVLALERATRGRAAYHGAAAKPL
                     PPQPLRGARAALACAACALPVLLGFLLPAAILLQLALGDPQARWGARLAGLVGNSFVI
                     SGVTAVAAVALAVTMAYAARLSKSRLAAAANRFAGLGYAMPGAVIAIGVLVPLGRLDN
                     WLAGMIEEATGERAGLLLTGTIAALVYACVVRFLAVALQTTEAGLARITPSMEDAARS
                     LGAGPASALARVHAPLLAPSLATAALLVFVDVLKELPATFALRPFNFDTLAVEAYHLA
                     KDERLAEAAVPSLVIVLVGLVPLLALSRRYFQATRS"
     gene            complement(10486..11508)
                     /locus_tag="VFB53_01655"
     CDS             complement(10486..11508)
                     /locus_tag="VFB53_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN85465.1"
                     /translation="MSVLKSLILIPALALSGPALAQDRVLNLYSSRHYQTDEALYSGF
                     TKLTGIRVNRIEAGEDALLERIRNEGARSPADVLVTVDAGRLWRAEQMGMFQPVKSAQ
                     LESRIPASFREPNGLWFGFSLRARVIAYNKSRVGAGELKDYQDLADAKWKGRLCMRSS
                     SNIYNLSLMGALIDHLGEAQAEAWAKAVRANLAREPRGGDTDQLKSVAAGECDVTIAN
                     QYYYARLARSQKADERAVAQKLGIVFPNQAGWGAHVNISGAGVMKHAPNREAAVRFLE
                     YLASDDAQRYFADGNNEWPVVASVQVANPVLQAFGAFKVDPLNVAVLGRNQPGSQKIY
                     DRVAWK"
     gene            complement(11584..12000)
                     /locus_tag="VFB53_01660"
     CDS             complement(11584..12000)
                     /locus_tag="VFB53_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZN85466.1"
                     /translation="MPRAETAPVGERRAEALLQTLLLLGLDYRYGGTSPETGFDCSGL
                     VAHVFREAYGIQLPRNARAQSEYGAKVSLAELRAGDLVFYNTLNQPFSHVGIYLGDGR
                     FVHAPKTGAQVRVEPLKGSYWMKRFDGARRIQPAAL"
     gene            12098..>13803
                     /locus_tag="VFB53_01665"
     CDS             12098..>13803
                     /locus_tag="VFB53_01665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZN85467.1"
                     /translation="MRATTYAAEALMQLFADGGPLAGQIPGYRLRPQQLELAAAILQA
                     IETDAPLVAEAGTGTGKTLAYLVPALLAGGKVIVSTGTKTLQDQLYDRDLPAVRAALA
                     SGAVTALLKGRANYVCLHRLERAGAEARLASRDEAAQLARIERFAALSASGDRAELPD
                     VPDDAPVWLHATSTRENCLGQECPRYQDCFVMKARRGAMAADVVVVNHHLFFADVVLR
                     DEGVAELLPSCNTLIFDEAHQLPDTARLFFGESVSTGQLVELSRDARLELRAAGGAGP
                     EFDPLAGRLEKAARDLRLCFGSDGARLAWAQAAARPEFGGALGALQAALTAFRAALAA
                     QAERAEGLASCARRAQEAHAVLERIAGAEDTALVRWVEVFGHALQLHLTPLSSAELFR
                     RQLEEQPRAWIFTSATLSVGEDFSHFQEELGLGAAAARSWPSPFDYARQALLYVPRGM
                     PADPNDPAFTDSVVEAALPVLEASGGRAFLLFTTLRALRRAHELLRERVKFPLLVQGT
                     GSRSELLARFRELGNAVLLGSASFREGVDVRGEALSLVVIDKLPFAPPDDPVLAARID
                     AIR"
BASE COUNT         1936 a         4925 c         4971 g         1971 t
ORIGIN      
        1 gagggcatgc cggcggtcga gtggtacaag ccgaccgagc gcgggctgga gggccgcatt
       61 cgggagaagc ttgcagagct caagcggctg aacgccgccg cgcgcgggaa aaagacatga
      121 tcgacattca actcctgcgc aattctcttg catccgttgt ggaacgactg aagacccgcg
      181 gctttgtcct ggagcccgcg gcatttcttg cgcttgagac tcgtcggaag gacatccaga
      241 ccaggacgga gtctcttcag gcacggcgga atacgctgtc caaggaagtc ggcatcgcaa
      301 aagcgaagaa agatccggca ggggcggacg cattgatgaa gcaggcagcg gctgccggcg
      361 atgagctcaa ggcgcttgaa gcggagaatc aactggtaca ggatcagctg cgcaatttcg
      421 tcgcgctgat acccaatctt ccccacgaga gcgttccagt cggcgcctcc tccgagcaga
      481 acgtcgaggt gcgccgccgc ggaaccccgc gcaagttcga cttcccggcg aaagaccacg
      541 tcgacgtcgg cacgccgctg ggcctcgact tcgaggccgg cgccaagctc gccggcgcgc
      601 gcttcgcggt gatgaagggc cagctcgcgc gcctgcaccg ggccctcgcg cagttcatgc
      661 tcgatgtgca caccaccgag catggctata ccgaggccta cacgccgtac atggtgagcg
      721 gcgcctgcgc cgacggcgtc tccagcctcg ccaagttcaa gggcgacctg ttcaagctcg
      781 aggaccgcga cctgtacctg atcccgaccg cggagtactc ggtcaccaac ttcgtgcgcg
      841 acgagatcct gccgctggaa tcgctgccgc tgaaatacgt ctgccattcg ccctgtttcc
      901 gctcggaggc gggcgccgcg ggcaaggaca cgcgcggcat gatccgcaac caccagttcg
      961 acaaggtcga gctggtgcag atcgcgcacc cgtcgcagtc ctacgcgctg cacgaggagc
     1021 tcacggcgca cgccgaggcg atcctgcagc agctcgggct gccctaccgc gtggtcacgc
     1081 tgtgcaccgg ggacacggga ttctccgccg ccaagaccta cgacctcgaa gtctggctgc
     1141 cggggcagaa cgcctaccgc gagatcagct cctgctcgaa cttcgaggcc ttccaggcgc
     1201 ggcgcatgca ggcgcgcttc aggaacgcgc agggcaagcc cgagccggtg cacacgctga
     1261 acggctcggg actggcggtg ggccgcacgc tggtggcgat cctggagaat ttccagaacg
     1321 ccgacggctc ggtgagcatc cccgcggcgc tgcgctcccg catgggcggg gcggagaagc
     1381 tcgcccggcc ctgatagaat cccgctgccg cggagagatg gcagagtggt ctaatgcggc
     1441 agactcgaaa tctgttgtac ggttatccgt accgggggtt cgaatccctc tctctccgcc
     1501 atcccttgcc cgagcccagg cgcccatgat cggcgtatac cgcgatatcg aaggctcgat
     1561 gcgcctggcg gggcacgtgc tgcgccgctt ctgggtgctg aagctgccgc cggtggccga
     1621 ggagctggtg cgccagatct acttcaccgg ggcgagcgcc accactacca tcgtgatccg
     1681 cggcgcgctg atcggcacgc tgatcatcgc ctacgtgatc gaggtgctca acgccgacgc
     1741 cgcgctggcg gtgaagctgc tgctgctggt ggtgctgcgc gagatcgggc cgctgatcgc
     1801 cgcggtgctg gtgatccagc gcagcggcac ggcggtggcc accgagctcg cgctgatgaa
     1861 gatctccggc gaggtggcga gcctgcgcca cctgcgcatc gacccgcagg actacctggt
     1921 ggtgccgcgc gtggcgggca tcgcgctcgc caacaccgcg ctcaccgtct acgtgcagtt
     1981 catcgcggtc ggcggcggca tggtgctcgc cgcgctggtg atcgacgcct cgctcgagga
     2041 gctggcggcc aacttcttcc tgctggccag cccgctcgac gtggtgtact ccgtcgccaa
     2101 gagcctgctg ttcggcgcgg ccatcggcgt catcgcctgc tattacggcc tcaacccgcc
     2161 gggcaattcg gtgaacgccg tacccaaggc ggcgatcagc gcggtgaccc aggcgacgct
     2221 ggcggtgatg ctgctcaacg tggtgttcgc ctacctggtg tacggctcgg cgctgttcgg
     2281 cctgttcacc gcggaccaat gagcgcggcc gcggaggtgg cgctgggcta cgagccgcag
     2341 gcgggatccg gccttcccgg ggagcgcatc tggatcgagg cgggcggcag ccatgtggtg
     2401 cccgcggtcg gcgccgcgct gaagccggtg ctgctcgccg cggtgatcga gctgcggccg
     2461 cgcaaggacg cgcgcgtggt ggtgctcggc gccgagaccg ggtcgctgcc ggagaaggcg
     2521 cgcgccgccc tgcgcgccca ggtcgccttc ctgccggcgg gcggcgggct gctcagcaac
     2581 ctcaatgcct gggagaacat cacgctgccg atcggctacc acgagccgaa gcgggcgcgc
     2641 ggcctcgcct cgcaggtcta cgccctgctg gagcagttcg gggctgacgc gcgcgcgctg
     2701 ctcgccaagc tgcccgaaag catgacgccg gacgaaagaa tgctcgccgg ctatgtcagg
     2761 atgcggctgg ggcggccgcg gctggtgctg ggcgaggatc cgggcggcgc ccccggcgcc
     2821 tccgagcggc ggcgggcggc ggctttcgcc gccgcctacc tggccgactg cccggaaggc
     2881 actttcgtcc agctgcagga agacgcatcg gacggctcgg gataatcgcg cggcgaaaag
     2941 ggaggtcgca atgcaggaac aacaggcact accccggcga tttccgggca tccggcggcg
     3001 catcagccta ttcctggtca ccgcgctcgt cggcctcgtc ggcgccgtgt tgctggtggc
     3061 ctacaagcag ggcgcgttcg tgcgccagac gtcgatcttt ttctacgccg aggacgtgat
     3121 cggcatcaac aagggcatga cggtgcgcct gttcgggctg ccggtgggca gcgtgaagca
     3181 cctggagatc accacccgcg gggtgcaggt cgagctgtcg atcgtcagcg agtacgtccc
     3241 gcgcctcacc aagggcacca atgccaggct gacccgcgaa ggctacatcg gcgcggccag
     3301 cctgctgctg ctgcccggcg gcgaggcgaa ggatgcgcgc gcaccgctgg cggcgggcga
     3361 cgtcatcggc tatctcccca atcgcggcgt cgccgaactg gtcgacgaca tcaagaacca
     3421 gctggcgccg gtggtggggg aactgcgcgg catgatggcg gaactgaacc gtcccgaggg
     3481 cgacttccgc aagtcgctgc agggcgcgag cgtggtcctg cagcagctgc ccgagaccaa
     3541 ccgcgagctg cgccagctgc tgcgcgacac cgaccgtgcc gtcaccgccg tcggcggccg
     3601 cgcggaggcg gcgctcgtcg ccaccgagcg cgcgggtacg cgcctggagc gcgagctgcc
     3661 cgagttgtcg cgccaggctg gcgcgaccct ccaggcggtc gccgaggcgg cgaccgagat
     3721 ccgcagcacc acgcgcaaga acggcgaggc gctgcaccag gcgctggtgc agaccccggc
     3781 gctggtgcgc gacggcgccc agctggtgcg cgacggccag gacgtggtcg gcgcggcgaa
     3841 gaacagctgg ctgatccgcg actacgtcga gccggccgcc atgcgcacgc tgccgctgga
     3901 cagcttcgaa gcgcagcgtt gatgcgcctc accgccgccg cggcctgcgc gctgctcgcc
     3961 gcctgcgccg cacgcacgcc cgcgccgccc gcgccgacgc aggccactgc cgtgcagccg
     4021 acggcggcga tggaggccaa ccgccgcgcc gaggcgagcc tgcggcgcgg cgagctggac
     4081 ggcgcggcgt tccactaccg cgaggcgctg cgcctgtcgc tcgcggtcga ggacgcggag
     4141 ggcatcgccg ccaatgccgt caacctgtcc atcgtgcatc agcgccaggg caagaccgag
     4201 gaggcgcgcg cgagcctcgc gcccctgctc gagcgcgcca cgctgcgcca tgcgccggtg
     4261 caccgtgcgc aggccgcgct gcgccgcgcg gtgctcgacc tggacgagcg gcgccatgcc
     4321 agcgccgccg agtgggcagc gcgcgccacg gactggtgcg gcaccccctg cgcgctcgcc
     4381 gggccgatcc gcaacgtgca gggccagctg gcgctggccg ccgggcggct cgaggaagcc
     4441 gcggcggctg ccgcgaacgc cctggcggag agccgcgccg ccggccaggc ggcggagatc
     4501 gccaacgcgc accggctgcg gggcatgacg gcgctcgccg ccggcgacgg cgcggccgcg
     4561 ctcgccgcgc tgggcgaggc cctcgccatc gaccgcgaac tcggcattcc gcgccgcatc
     4621 ttcctcgacc tggtcggcct gggcggcgcg agcaagctgc agggcgacag gcaggccgcg
     4681 cgcggctact atgcgcgcgc ccaggccgtc agcgaagccg accgcgatgc cgccgggagc
     4741 gcagaggcgc gcgccctgct cgatgagctg gacctggcaa gcaggaaatg aacgcacccg
     4801 ccgtccagtt cgaccccgca gccgcgcgct tcggctccgc gcgcacgcaa aagcgcctcg
     4861 aggacgaccg cctgctcgtc ggcaagggcc tgtacagcga cgaccggcgc ttcgagggcg
     4921 aagcggccct ggtgctggtg cgctcgccct acgcgcacgc gaagatcagg tcgatcgacc
     4981 tcgccgcggt gcgcgccgcg ccgggcgtgc tcgccgcctg gaccatggcg gagctcgccg
     5041 ccgatggcgt gcagcacatt cccacgccgc ccctgttcaa gcgcgccgac gggcagccga
     5101 tgcaggcgcc gctgcgcacg ccgctcgccg gcgagcgcgt cctctacgtg ggccacccgc
     5161 tggtcgccgt cgtcgcgcag acgcgcgcgc aggcgcagga cgcggccgag ctcgccgcga
     5221 tcgatttcga ggagctgccc tgcgtggccg atgcgcgcgc cgccgtgaag ccgggcgcgc
     5281 cgcagctcgc gccgggcgcg ccgggcaatg tcgccgccga gacgcgcttc ggcaagcccg
     5341 acgaggcgcg ggcggctttt gcccgcgcgg cgcacgtcac cgaaatcgag ctgcacaacc
     5401 agcgcgtcat cgccatggcg ctggagccgc gctgcgcgat cggcgtgcac gagaacgggc
     5461 gcaccaccgt ctacacccag tcccagcagc ccaccggcgc gcgcgacggg ctcgccgccg
     5521 cgctcggcgc gaagccggcg gacctgcgca tcgtgaacgg cgacatcggc ggcggcttcg
     5581 gcatgaagac cggcgccacg cccgaggagg cgctggtcgc ctacgccgcg aagaagctcg
     5641 gccgcccggt gcgctggcgc ggcgaccgga gcgaggagtt cctcgccgcg cacatgggcc
     5701 gcgacatgca tttcaaggcg gcgctcgcgc tcgacaggga cggccgcatc ctcgcgctgc
     5761 gcctgcactc gctgggcaac atgggcgcgg tgccggtgaa ttcctcggcc atgattccgt
     5821 tgatgatggt ggccaaggtg gcgacctcgg tgtaccgcgt gccggcggtg gactaccact
     5881 tccagggcgt gctcaccaac accatggcca ccggcgccta ccgcggcgcg gggcggccgg
     5941 aaggcaattt cgtcatcgag cggctggtgg agaaggccgc gcgcgaaatg cgcatcgacc
     6001 cggtcgagat gcgccggcgc aatctgctgc agccggggga tttcccggtg gccacgcacg
     6061 ccggcgaggt ctacgacacg ggcaacttcc ccgggatcct ggacggcctg gtggagctcg
     6121 ccgactggaa gggcttcccc gcgcgcgcgg cggaatcgaa gcggaggggc aggctgcgcg
     6181 gccgcgggct ggcgacctac ctggagtgga ccggcgcgct gccgacggag accgtcgaca
     6241 tcgaagtcga cgccgcgggc cgggtcaccg tgttctccgg cacccaggcc atggggcagg
     6301 ggctggagac gagctacgtg cagctcgtca ccgaggtgct gcagatcccg gcggcgcgcg
     6361 tgacgatcgt gcagggcgac accgaccgcg ccaacggcgt gggcagcgtg ggctcgcgct
     6421 cggccttcgt cggcggctcc gcggtggtcg ccgcgggcca cacgctcgtc cggcgcggca
     6481 gggaactcgc cgccgacgcg ctcgaggcgg cggcgcagga catcgagttc cgcgacgggc
     6541 gcttcaccat cgccggcacc gacaagtcgc tcgatctcgc ggggctggcg gcgcggcagg
     6601 cggcgaaaat cctgcgcgtg tcggcgacgc agacgccgtc cacgccttca tggccgaacg
     6661 gcgcgcaggc ctgcgaggtg gagatcgacc cggagaccgg cgaagtggcg ctcgcgcgcc
     6721 tgacgagcat ggacgacatc gggcgcatca tcaaccgcat gatcgtcgag gggcagatcc
     6781 acggcggcat ggcgcagggc gtgggccagg cgctgtacga gcaggcggtg tacgacgaga
     6841 gcggacagct gctcaccggc tcgctgatgg actactgcgt gccgcgcgcc gaccagttcc
     6901 cgcccctcgc cgcggcgttc gacgagagcg tgccctgcac caccaacctg ctcggcgtga
     6961 agggctgcgg cgagatcggc accatcggcg cggtgcccgc ggtggtgcac gcggtgctgg
     7021 atgcgctggc cgaattcggc gtcgtgcacc tggagatgcc gctgaccccc gagaaggtat
     7081 ggcgggcgat gcggcgcgcg tagcggacat gcgcggcggc gcggaccgcg cgtttgcgct
     7141 cgccctcctc gcgtcgatcc tgctgcacgc gctgctgctg ggcgcgctgc cgtcctggcg
     7201 cgcgccgccg gagccgccgg cggccctgcc gctgaccgcg cgcctcgccg aggcccctgc
     7261 ggcgccggcg tccgcgccgc ccgcacccgt gtcggtggag gaggacgcgc gcccggcgcc
     7321 gcccgccgcc gctcgcgcgc cgctacggcg ccccgcgccg agccccgctc gggcagcgct
     7381 tcccgtgcaa ccggcattga gcgagcgcgc gctggcgccg ccggcgccgc tcgcgcccgc
     7441 cgcacccggg cccgtggcgc gcatcgaccc gctgccggcc gcgccgccgc cggccgagag
     7501 cgatgccctg gcgacctacc gcgcgaacgt caacgccgcg gccacgcgct tcaagcgcta
     7561 cccgcgctcg gccatcgaca acaactggga aggcgaggtg gtggtgctga tgagcatcgg
     7621 cgccgacggc cgcatcgcct cgctgcgcgt gcgcaccagc tcgggctacg aagtcctcga
     7681 ccggcaggcg ctcgacatgt tcaggaacgc caagcccttc gtgccgctgc cgcgcgagct
     7741 gcgcgggcag gagttcgagg tcgagttgcg cgcgatctac agcctcaagg atcagggctc
     7801 gggctgaaac gccaccacgt ggtcgacgtc gaggcggatg ccgatgcgct cgcccagcgc
     7861 gtggttgtgg tggctgggca ccagcgccag gaccttgcgc ccgctcgcca ggcgcagcgt
     7921 gtagaggatc tccgcgccgc ggaacgcctt gtgcagcacc tcggccttga gcgccgaggc
     7981 gtcgtcatgc accacgtcgt cgggccgcag caggatctcc gcggcgccgc cggggatctc
     8041 gccttcgatc acgccgagct cgatctcgac ctggcgcgcg ccgagcctgc gcgccggcag
     8101 gaacaccccc tggccgacga agtcggcgac gaagcggctc gccgggcggt ggtacaggtt
     8161 gtaggcgctg tcccactgca cgatgcggcc ctcgtgcatc acgccgatct cgtcggccac
     8221 cgcgaatgcc tcgtgctggt cgtgcgtcac cagcaccgcg gtggcgccgc tcgccttgat
     8281 gatgtcgcgc acttccagcg acaggcgctc gcgcaggtcc acgtcgaggt tggagaacgg
     8341 ctcgtccagc agcagcagtt cggggcgcgg cgcgagcgcg cgcgccagcg ccacgcgctg
     8401 ctgctggccg ccggagagct cgtgcgggta gctggcgagg accgcggcga ggccggtcag
     8461 atcggccagc tcgcgcacgc gcgccgcgcg cgcggcggcc ggcagcgccc gcagcgcgaa
     8521 ggcgatgttg cgctcgaccg agaggtgcgg gaacagcgcg tagtcctgga acaccatgcc
     8581 gacgtgcctg cgctccggcg gcagcatgct gcccgggccg gaaaccagca cgccgttcag
     8641 ccggatgctg ccggcgcgga tcgcctcgaa gccggcgatg cagcgcagga ccgtcgtctt
     8701 gccgcagccg ctgggaccga gcaggcagcc gatggcgccg cgcggcagcg cgaagtccag
     8761 cccgcggacc acttcggcgc cgttgtagcc gtgatgcaac ccctggacct cgagctggtt
     8821 cacagggcga ttataagctg aaccgtaaca ataatcgttc ttgttagaat ggcgagtgct
     8881 tttctatcaa acagttaggc gagggggcag cggcctgtcg ctggcgatcg ccgctctgct
     8941 ggccctcccg atcctgtggg tggcgagcag cgtgctgcag cccgcgggtg atgcctggcg
     9001 ccatatcgcg gcgaccttgc tgccgctgta cgcggccaac accgccttgc tgctcgcgct
     9061 ggtcgcctgc ggcgtgatct cgatcggcgt gctgtcggcc tggctggtgg cgagcacccg
     9121 cttccccggc gtgcggctgc tggaatgggc gctcttcctg ccgctggcga tgcccgccta
     9181 cgtcatggcc tacgcctaca ccgactggct gcagtttacc ggcccggtgc aggaggccct
     9241 gcgctcgctc accggctggc aggcgcgcga gtactggttt cccgagatcc gctcgctggg
     9301 gggcgccgcc gcggtgctct cgctcgcgct ctatccctac gtgtacctca tcgcgcgcgc
     9361 cgccttccgc gagctgccgc gcagcgccat cgaagcgggg cgcctcgccg gccattccgc
     9421 ggggcgcaac ttcctgcgcg tcgcgctgcc gctggcgcgc ccggcgatcg ccgccggcac
     9481 cgcgctcgcc ctgatggaga cgctcgccga tttcggcacc gtgtcctact tcgcgctcga
     9541 ggtgttcacc accggcatct tcaaggcctg gctgtcgatg ggcgacagcc tcgccgcggc
     9601 gcagctctcg agctgcctgc tcgcgttcgt gctgctggtg ctggcgctgg agcgcgccac
     9661 gcgcggccgc gccgcctacc acggcgccgc cgccaagccc ctgccgccgc agccgctgcg
     9721 cggcgcgcgc gcggcgcttg cctgcgcggc ctgcgccctg ccggtgctcc tcggattcct
     9781 gctgccggcg gcgatcctgc tgcagctcgc gctcggcgac ccgcaggcgc gctggggtgc
     9841 gcgcctcgcg gggctggtcg gcaacagctt cgtcatctcc ggcgtcaccg cggtcgccgc
     9901 cgtggcgctg gcggtgacca tggcctacgc ggcgcgcctg tcgaagagcc gccttgccgc
     9961 ggcggcgaac cgcttcgccg ggctcggcta cgccatgccc ggcgcggtga tcgccatcgg
    10021 cgtgctggtg ccgctggggc gcctggacaa ctggctcgcc ggaatgatcg aggaggccac
    10081 cggcgagcgc gcgggcctgc tcctcaccgg gaccatcgcc gcgctggtgt acgcctgcgt
    10141 ggtgcgcttc ctcgccgtgg cgctgcagac caccgaggcg ggcctcgcgc gcatcacgcc
    10201 gagcatggag gacgccgcgc gcagcctggg cgcggggccg gcgtccgcgc tcgcccgcgt
    10261 gcacgccccc ctgctcgcgc ccagcctcgc caccgccgcc ctgctggtgt tcgtcgacgt
    10321 attgaaggaa ctgcccgcca ccttcgcgct gcgccccttc aatttcgaca cgctggcggt
    10381 cgaggcctat cacctggcga aggacgagcg gctcgccgag gccgccgtac cctcgctggt
    10441 gatcgtgctg gtcggcctcg tgccgctgct ggcgctgtcg cgtcgctact tccaggccac
    10501 ccggtcgtag attttctgcg agccgggctg gttgcggccg agcaccgcga cgttgagcgg
    10561 gtcgaccttg aaggcgccga aggcctgcag caccgggttc gccacctgca cgctcgccac
    10621 caccggccac tcgttgttgc cgtcggcgaa gtagcgctgc gcgtcgtcgc tcgccaggta
    10681 ctcgaggaag cgcaccgccg cctcccggtt gggcgcgtgc ttcatcacgc ccgcgccgga
    10741 gatgttgacg tgcgcgcccc agcccgcctg gttggggaac acgatgccga gcttctgcgc
    10801 caccgctcgc tcgtcggcct tctgcgagcg cgcgaggcgc gcgtagtaat actggttggc
    10861 gatggtcacg tcgcattcgc ccgcggccac gctcttcagc tggtcggtgt cgccgcccct
    10921 gggttcgcgc gccaggttgg cgcgcaccgc cttcgcccag gcctcggcct gcgcctcgcc
    10981 caggtggtcg atcagcgcgc ccatcagcga caggttgtag atgttgctgg aggagcgcat
    11041 gcacaggcgg cccttccatt tcgcgtcggc aaggtcctgg taatccttga gctcgcccgc
    11101 gccgacccgg gacttgttgt aggcgatcac gcgcgcgcgc agcgaaaagc cgaaccacag
    11161 gccgttcggc tcgcggaagc tcgccgggat gcgggactcg agttgcgccg acttcaccgg
    11221 ctggaacatc cccatctgct cggcgcgcca caggcgcccg gcgtccacgg tcaccaggac
    11281 gtcggccggg ctgcgcgcgc cctcgttgcg gatgcgctcg agcagcgcgt cttcgcccgc
    11341 ctcgatgcgg ttcacccgga tgccggtgag cttggtgaag ccggaataga gcgcctcgtc
    11401 ggtctggtaa tgccgcgagg agtagaggtt gagtaccctg tcctgggcga gcgcagggcc
    11461 ggagagggca agggcaggga tgaggattag gcttttgaga acagacattt gggccacatt
    11521 tgaaaaagag actcaaatgg taatgactct cattgagaga gtcaactcaa atgagaactg
    11581 tttctaaagt gccgcgggct ggatgcggcg ggcgccgtcg aagcgcttca tccagtagct
    11641 gcccttgagg ggctcgacgc gcacctgcgc gccggtcttg ggcgcatgca cgaagcgccc
    11701 gtcgccgaga taaatgccga catgcgagaa cggctggttg agcgtgttgt agaacaccag
    11761 gtctccggcg cgcagttccg cgaggctgac cttggcgccg tactcgcttt gcgcgcgcgc
    11821 gttgcgcggc agctggatgc cgtaggcttc gcggaacaca tgcgccacca gaccgctgca
    11881 atcgaagccc gtttcgggcg aagtcccgcc gtagcgatag tccaggccga gcaggagcag
    11941 ggtctgcagc agggcctcgg cgcgccgctc gccgaccggg gctgtctccg ccctgggcat
    12001 ttcgggcggc aaggtggagc agcccaccgg aatcaccaaa gccagtaaat acaggtattt
    12061 agctgccata tctcatgcaa tctagtccaa tgttcgaatg agagcaacca cttacgccgc
    12121 cgaagcgctg atgcagctgt tcgccgacgg cggcccgctg gcggggcaga ttccaggcta
    12181 ccgcctgcga ccgcagcagc tcgagctggc cgcggcgatc ctgcaggcga tcgagaccga
    12241 cgcgcccctg gtggccgaag ccggcaccgg gaccgggaag accctcgcct acctggtgcc
    12301 ggcgctgctg gcgggcggca aggtcatcgt ctccaccggc accaagaccc tgcaggacca
    12361 gctctacgac cgcgacctgc cggcggtgcg cgcggcgctc gcctccgggg cggtgaccgc
    12421 gctgctcaag gggcgcgcca actacgtctg cctgcaccgc ctggagcggg ccggcgccga
    12481 ggcgcgcctg gcgagccgcg acgaggcggc gcagctcgca cgcatcgagc gtttcgccgc
    12541 gctgagcgcg agcggcgacc gcgccgagct gcccgacgtg cccgatgacg cgccggtctg
    12601 gctgcacgcc acctcgacgc gcgagaactg cctggggcag gaatgcccgc gctaccagga
    12661 ctgcttcgtg atgaaggcgc gccgcggcgc gatggccgcc gacgtggtgg tggtgaacca
    12721 ccacctgttc ttcgccgacg tggtgctgcg cgacgaaggc gtggccgagc tgctgccgtc
    12781 ctgcaatacg ctgatcttcg acgaggcgca ccagctgccg gacaccgcgc ggctgttctt
    12841 cggcgaaagc gtctccaccg ggcagctggt cgaattgtcg cgcgacgcgc gcctggagct
    12901 gcgcgcggcg ggcggggcgg ggccggagtt cgacccgctc gccggccggc tggagaaggc
    12961 ggcgcgcgac ctgcggcttt gcttcggcag cgacggggcg cggctcgcat gggcgcaggc
    13021 ggcggccagg ccggagttcg gcggcgcgct cggcgcgctg caggcggccc tgacggcatt
    13081 ccgcgcggcg ctggccgcgc aggccgaacg cgccgagggc cttgcctcct gcgcgcgccg
    13141 cgcgcaggag gcgcatgccg tcctcgagcg catcgccggc gccgaggaca cggcgctggt
    13201 gcgctgggtg gaagtgttcg gccatgcgct gcagctgcac ctgacgccct tgtcgagcgc
    13261 ggagctgttc cgccgccagc tcgaggagca gccgcgcgcc tggatcttca cctcggcgac
    13321 gctctcggtg ggagaggatt tctcgcactt ccaggaggag ctcggcctgg gggcggcggc
    13381 ggcgcgcagc tggccgagcc ccttcgacta cgcccgccag gcgctgctct acgtgccgcg
    13441 cggcatgccc gccgacccga acgacccggc gttcaccgac agcgtggtcg aggcggcgct
    13501 gccggtgctg gaggcgagcg gcggccgggc gttcctgctg ttcaccaccc tgcgcgccct
    13561 gcgccgcgcg cacgagctgc tgcgcgagcg cgtgaagttt ccgctgctgg tgcagggcac
    13621 cggctcgcgc agcgagctgc tcgccaggtt ccgcgagctg ggcaacgcgg tgctgctggg
    13681 cagcgcgtcg ttccgcgagg gcgtcgacgt gcgcggcgag gcgctgtcgc tggtggtgat
    13741 cgacaagctg ccgttcgcgc cgcccgacga tccggtgctc gcggcgcgca tcgacgcgat
    13801 ccg
//
LOCUS       DASRIE010000036         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_811533, whole genome shotgun sequence.
ACCESSION   DASRIE010000036 DASRIE010000000
VERSION     DASRIE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811533"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFB53_01670"
     CDS             <1..987
                     /locus_tag="VFB53_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HZN85468.1"
                     /translation="LHFTFTQPETHGFAGFLFAVLTGFDKPFNQAPSLHIALLVILWV
                     LYARHVPRWALWLLHPWLVGVSVLTTYQHHFIDIPTGALLGFFCLWLWPDRGESPVAA
                     FQLTGDRRRMVLAARYLAGAIATAALALWIGGAGWWLFWPAISLLLVAANYAALGAGG
                     FQKGPDGDMSLAARVLLAPYLLGAWINSRAWTRRESAPVIVADGVALGRLPSRRGAAD
                     FASVIDLSAELPRPEQHVAWRAFPTLDLVVPQPQLLRAAAQAIEEGRARGPVLVCCAL
                     GYSRSASALATWLSAYGHAGTVATAVARIRRARPQVVLGDATVAAIGRAAAP"
     gene            1026..1439
                     /locus_tag="VFB53_01675"
     CDS             1026..1439
                     /locus_tag="VFB53_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85469.1"
                     /translation="MALLDQGRGIDRLSRLLTAAALAALVGIAVWGVHHPVPVSLLAL
                     SVLAGVVEVYLAIRVGFDAALFRHLADAAETGTRDLANLDAALTAIGLVPASKAGRPL
                     EARVAGACRLFYQQAGSVALQVALFLLGAVTAALT"
     gene            1451..2092
                     /locus_tag="VFB53_01680"
     CDS             1451..2092
                     /locus_tag="VFB53_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN85470.1"
                     /translation="MARAIESLAAMAVIGFARLVTGVRGNWRDCAPEARPRVYFANHN
                     SHGDFVLIWTVLPPALRRVTRPVAGADYWTVRPLRRFFGDRVFRAVLIDRNPETRQGD
                     PIALMANALDAGSSLILFPEGTRNMSEEPLLPFKSGLYHLANARRQVELVPVWIENLN
                     RVMPKGEFVPIPLLCTVTFGAPLNVRDGEDKDAFLGRARTALLDLAPEREGSR"
     gene            2089..>2672
                     /locus_tag="VFB53_01685"
     CDS             2089..>2672
                     /locus_tag="VFB53_01685"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN85471.1"
                     /translation="MTATEQTLALFGGIAAILVVASAIGFVLQRRYAAAGPNAVIENL
                     NSRIKSWWVMVIAIGVAFAFGKAGVIALFGFASFTALREFVTLLSTRRSDHLALVIAF
                     FVILPAQYYFIWIEWYGLYSIFIPVYAFLLLPIIAAARGDTKAFMPRVAEVQWALMIC
                     VFCVSHVPALLTLHIPGYEGRQLLLIAFLVLVVQS"
BASE COUNT          312 a          964 c          915 g          481 t
ORIGIN      
        1 ttgcatttca ccttcaccca acctgagacc cacggtttcg cgggctttct gttcgcggtg
       61 ctcaccggct tcgacaagcc gttcaaccag gcgccgtcgt tgcacatcgc actgctggtg
      121 atcctctggg tgctctacgc ccgccacgtg ccgcgctggg cgctctggct gctgcatccc
      181 tggttggtcg gcgtctccgt gctcaccacc taccagcacc atttcatcga catcccgacc
      241 ggcgcgctgc tcggcttctt ctgtctctgg ctgtggccgg accgcggaga gagcccggtc
      301 gcggccttcc agctcaccgg cgatcgccgc cgcatggtgt tggcggcccg ctatttggcc
      361 ggcgcgatcg ccaccgccgc gctggcgttg tggatcggcg gcgccggctg gtggctgttc
      421 tggcccgcga tctcgctgct gttggtggcg gcaaactacg cggcgttggg cgccggcgga
      481 ttccagaagg gcccggacgg ggacatgagc ctggcggcgc gcgtcctgct ggcgccgtat
      541 ctcttgggcg cctggatcaa ttcacgcgcc tggacccgcc gggagtcggc gccggtcatc
      601 gtggcggacg gcgtcgcgct cgggcgcctg ccgtcccgcc gcggcgcggc tgatttcgcc
      661 tccgtgatcg acctgagcgc ggaactgccg cgcccggagc agcacgtcgc gtggcgcgcg
      721 ttcccgaccc tggacctggt ggtgccgcag ccgcagctgt tgcgcgcggc cgcgcaggcg
      781 atcgaagagg gccgtgcgcg cggtccggtg ctggtctgct gcgcgctcgg ctactcgcgc
      841 agcgcctcgg cgctggcgac ctggctcagc gcctatggac atgccggcac cgtcgcgacc
      901 gcggtcgcgc gcatccggcg ggcgcgcccg caggtggtgc tcggcgacgc caccgtggcg
      961 gcgatcgggc gtgccgcggc gccatgatgg tcgcagcccc agcgggtcag ctgctggtga
     1021 gcgcagtggc cctgctcgac cagggtcgcg gtatcgatcg gctctcgcgc ctgttgaccg
     1081 cggccgcgct ggccgcgctc gtcggcatcg cggtctgggg cgtgcatcat ccggtgccgg
     1141 tgtcgctgct ggcgttgtcc gtgctcgccg gcgtcgtcga ggtctatctg gcgattcgcg
     1201 tcggcttcga cgccgccctg ttccgccacc tggcggatgc ggccgagacc ggcacgcgcg
     1261 atctggcgaa cctcgacgcc gcgctgacgg cgatcgggct cgtcccggcg agcaaggccg
     1321 gccggccgct cgaagcgcgc gtcgccggcg cttgcaggct gttctaccaa caggcaggct
     1381 cggtcgcgct gcaggttgcg ctgttcctgc tcggcgcggt caccgcggca ctgacctgag
     1441 gacgcccgca atggcgcgcg cgatcgaatc cctggcggcc atggcggtga tcggcttcgc
     1501 ccggctggtc accggggtgc gcggcaattg gcgcgactgc gcgcccgagg cgcgtccgcg
     1561 cgtctatttc gccaatcaca acagccacgg cgacttcgtg ctgatctgga cggtgctgcc
     1621 gccggccctg cggcgggtga cccggccggt ggccggcgcc gactattgga ccgtgcgccc
     1681 gctgcgccgg ttcttcggcg accgcgtatt ccgcgcggtg ctgatcgacc gcaatccgga
     1741 aacccggcag ggggacccga ttgccttgat ggcaaacgcg ctcgacgccg gctcctcgct
     1801 gatcctgttc ccggaaggca cgcgcaacat gagcgaggag ccgctgctgc cgttcaagtc
     1861 cggcctctac cacctcgcca acgcacggcg gcaggtcgag ctcgtcccgg tgtggatcga
     1921 gaacctcaac cgcgtcatgc ccaagggcga gttcgtgccg atcccgctgc tttgcaccgt
     1981 caccttcggc gcgccgctca acgtgcgcga cggcgaggac aaggacgcgt tccttggccg
     2041 cgcgcgcacc gcgctcctcg acctcgcgcc cgagcgcgag ggcagccgat gaccgcgacc
     2101 gagcagacgc tcgcgctgtt cggcggcatc gccgcaatcc tggtcgtcgc ctcggcgatc
     2161 ggattcgtgc tgcagcgccg ctacgccgcc gccggaccca acgcggtcat cgagaacctc
     2221 aacagccgca tcaaatcgtg gtgggtgatg gtgatcgcga tcggcgtcgc cttcgcgttc
     2281 ggcaaggccg gcgtcatcgc gctgttcggc ttcgcctcgt tcaccgcgct gcgcgaattc
     2341 gtcaccctgc tgtcaacccg ccgcagcgac catctggcgc tggtgatcgc cttcttcgtc
     2401 atcctgccgg cgcagtacta cttcatctgg atcgaatggt acgggctcta ctcgatcttc
     2461 atcccggtct acgccttcct gctgctgccg atcatcgcgg cggcgcgcgg cgacaccaag
     2521 gccttcatgc cgcgggtcgc cgaggtgcaa tgggcgctga tgatctgcgt gttctgcgtc
     2581 tcgcacgtgc cggcgctgct cacgctccac atccccggct acgagggccg gcaattgctg
     2641 ctgatcgcct tcctggtgct ggtcgtgcag tc
//
LOCUS       DASRIE010000037         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2379414, whole genome shotgun sequence.
ACCESSION   DASRIE010000037 DASRIE010000000
VERSION     DASRIE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379414"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..281
                     /locus_tag="VFB53_01690"
     CDS             138..281
                     /locus_tag="VFB53_01690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85472.1"
                     /translation="MSTRTRSPAFPDQEEMRAIMERAGFADVRYRNLMFGVACIHMAT
                     KPV"
     gene            391..3270
                     /locus_tag="VFB53_01695"
     CDS             391..3270
                     /locus_tag="VFB53_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN85473.1"
                     /translation="MTGPQQFMRLRFDRFEVDEADARLTRSGTPVALAPKPFAVLCAL
                     ARSPRMLVTKNALLDSVWGHRFVSESVLKSTVSELRAVLGDDPRRPRFIETVSRRGYR
                     FIAALDNPPPAPGEAEPATALAQMVGRARELERLRAAWRVAACGRHQTVWIAGEAGVG
                     KTTLIEDFAAEVGASHCARGQCVEPYGAAEPFMPVLEALTELCRRDAALVELMRAVAP
                     TWLVQLPWLSSVAEREALRRELNGSGQARMLREMGELVDRYTENRPLLLVTEDLHWAD
                     QATVQLIDYLARRRGGARLLWLGSFRLTEIIAADHALRAVRHELRLHGLAEEIVLDSF
                     SEQEVAEYLARRIPALAADENFVRELHRLTDGLPLFVADVVSDLAGRDDCGVAGASSA
                     LRRVRSTALPETLAGIIERYVRQLTPDQRALLDAASVWGVQFRLGTLARVLERDVASL
                     AETCAELARRQRWLSELPLPADVALADAGYAFRHELYRKALYQRIGAPTRAELHRKLA
                     AALERERAEGGQVAVAELAFHFELGHAPLPALRCYAEAAESALLRFSPSETLRLTERA
                     MALLPAADESHERAALEVTLATLSGTAAVQLLGTGSIRAKAAYERATRLLERVPQHPL
                     RALCLHGLALVLCLRGELGEAHALAQRAEALAAQTGDGAVLLCACLAHGLLEHLRGRP
                     RSAREWLERGAAAAESFDETAPAAVFTADPGVLVLCLLAIELLHLGLADQGRSRLQQA
                     HARARALREPGPQVAVLWLASLFHVLAGEPGRVAEASARMLEMIEKYALPQGRAAELW
                     FRGWTEAQLGDPRAGHRLIREGCDRAARLGIQGWASETLGYAAEALARAGDWASARRE
                     LDAAMQCAEATGEGKYRTQLLVLEGRIADALGEPDRARESLRQALAEARAREAPWLEM
                     IALSALCERAGASAEHRAALRRVLDGMTEGLDLPPVAKARALTEA"
     gene            3346..>3745
                     /locus_tag="VFB53_01700"
     CDS             3346..>3745
                     /locus_tag="VFB53_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85474.1"
                     /translation="MLGALVVALAVWILHSFLPALLVACVTAVATWPLYRRFAALLQP
                     RMSRGAISLAFTVMVLALVLAPLIFAGSALLAKASALLLGMVAADKAGIGVPLWLESV
                     PLAGSWLAEHWRSEFAHPGAIREWVEQRADP"
BASE COUNT          493 a         1324 c         1361 g          567 t
ORIGIN      
        1 cggccactac gcggtattca aggccgtgaa cgccgcttgg gcggcgcacg gcgccgaagc
       61 gtacttctgg aactgatcag gcacgccgaa agaaggatcg cgatcagccg cgctcgacac
      121 gcgcggcctt cgtcccgatc agcacccgca cgcgatcgcc ggccttcccg gaccaggagg
      181 agatgagggc catcatggag cgcgccgggt tcgcggacgt gcgctaccgc aacctcatgt
      241 tcggcgtcgc ctgcatccac atggcgacca agccggtctg aggccggcac ctcggccgcc
      301 aagcgaactg cgtttgatct agcgcaaggg cccgcgggcg tcgcttgcgt accttgcggg
      361 tgccagcctt ttgcaggacc cggagggccc atgacaggac cgcagcaatt catgcggctg
      421 cgcttcgacc gcttcgaggt cgacgaggcg gacgcgcggc tgacgaggtc gggcacgccg
      481 gtagcgcttg cgcccaagcc gttcgccgtg ttgtgcgcgc ttgcccgcag cccgcgaatg
      541 ctggtcacca agaatgccct gctcgacagc gtctgggggc atcgcttcgt gtccgaatcg
      601 gtgctcaagt ccaccgtcag cgagctgcgc gcggtgctgg gcgacgatcc caggcgcccg
      661 cgcttcatcg agacggtttc gaggcgcggc tatcgcttca tcgccgcgct cgacaacccc
      721 ccgccggcgc cgggcgaagc cgagcccgcc acggccctcg cgcagatggt cggccgcgcg
      781 cgcgagctcg agcggctgcg cgcggcatgg cgggtggcgg cctgcggccg gcaccagacg
      841 gtgtggatcg ccggcgaagc cggcgtcggg aagacaacgc tgatcgagga cttcgcggcc
      901 gaggtgggcg cgagccattg cgcccgcgga caatgcgtcg agccgtacgg cgcggccgag
      961 cccttcatgc ccgtgctcga ggcccttacg gagctgtgcc gccgcgatgc cgcgctggtc
     1021 gagctgatgc gcgcggtggc gccgacctgg ctggtgcagc tgccctggct gagcagcgtc
     1081 gcggaacgcg aggccttgcg gcgcgagctg aacggctcgg gccaggcgcg catgctgcgc
     1141 gaaatgggcg agctggtcga ccgctacacg gaaaatcgcc cgctgctgct ggtcaccgag
     1201 gatctgcact gggccgatca ggccaccgtg cagctcatcg actacctggc gcgccggcgc
     1261 ggcggcgcgc ggctgctgtg gctcgggagt ttccgcctca cggaaatcat cgccgccgac
     1321 cacgccctgc gggccgtgcg ccacgagctt cgcctgcacg ggctggccga ggagatcgtg
     1381 ctcgacagct tctccgaaca ggaagtcgcc gagtatctcg cgcggcgcat tccggcgctc
     1441 gcggccgacg aaaatttcgt gcgcgagctg catcgtctta ccgacgggct gccgctgttc
     1501 gtagccgatg tggtaagcga tctcgccggc cgggacgact gcggcgtcgc cggcgcgtcc
     1561 tccgccttgc gccgtgtgcg ttcgacggcg cttccggaga cgctcgccgg catcattgag
     1621 cgttacgtcc ggcagctcac cccggatcag cgcgcgctgc tcgatgcggc gagcgtatgg
     1681 ggcgtccagt tccggctcgg cacgctggcg cgggtgctcg aacgcgacgt cgcgtcgctt
     1741 gccgaaacct gcgcggagct tgcgcgtcgg cagcgctggc tgagcgagct gccgttgccc
     1801 gcggacgtgg cgctcgccga cgcaggctac gctttccggc acgagctgta tcgcaaggcg
     1861 ctgtaccagc gcatcggcgc gcccacccgt gcggagcttc accgcaagct cgcggccgcg
     1921 ctggagcgcg agcgggccga gggcggccaa gtcgcggtgg cggagctcgc gttccatttc
     1981 gagctcggcc acgctcccct cccggcgctg cgctgctacg ccgaagccgc ggagtccgcg
     2041 ctgctgcgtt tcagcccttc ggaaacgctg cgcctgacgg aacgcgcgat ggcgcttctc
     2101 ccggcggccg acgagagcca cgagcgcgcc gcgctcgagg tgacgctcgc cacgttatcc
     2161 ggcacggcag cggtgcagct tctcggcacg ggctcgatcc gcgcgaaggc cgcctacgag
     2221 cgcgcaacgc gcctgctgga gcgcgtgccg cagcatccac tgcgcgcttt gtgcctgcac
     2281 gggctggcgc tggtgctttg cctgaggggc gagctgggcg aggcgcacgc cctcgcgcag
     2341 cgcgccgaag cgctcgccgc ccagaccggg gatggagcgg tgctgctctg cgcctgcctc
     2401 gcccatgggc tgctcgagca cctgcgcggt cggccgcgca gcgcacgcga atggctggag
     2461 cgcggggccg ccgccgccga aagcttcgac gaaaccgcgc cggccgcggt gttcaccgcc
     2521 gacccgggcg tcctcgtgct gtgcctgctc gcgatcgagc tgctgcacct tggactcgcc
     2581 gaccaggggc gctcgcgcct gcagcaggcg cacgcccgcg cgcgggcttt gcgcgagccc
     2641 ggtccgcagg tggcggttct ctggctcgct tcgctgttcc atgtgctcgc gggtgaaccc
     2701 gggcgcgtgg cagaagcgtc cgcgcgcatg ctggagatga tcgagaagta cgcgcttccg
     2761 cagggccgtg ctgccgagct gtggttccgc ggctggaccg aggcgcagct tggcgacccg
     2821 cgcgccggcc atcgcctgat ccgcgagggc tgcgaccggg ccgcccggct cggcatacag
     2881 ggctgggcga gcgagacgct gggctacgcc gccgaagcgc tcgcgcgcgc cggcgactgg
     2941 gcgtcggccc ggcgggaact cgacgcggcg atgcagtgcg ccgaggccac cggcgaaggc
     3001 aagtaccgga cgcagttgct cgtgctcgag ggccggatcg cggatgcgct cggcgagccc
     3061 gatcgggcgc gcgaatcgct gcggcaggcg cttgccgaag cgcgcgcccg ggaggcaccc
     3121 tggctcgaga tgatcgcctt gtcggcgctt tgcgagcgcg ccggcgccag cgccgagcat
     3181 cgagcggcgc tgcgccgcgt gctcgacggg atgaccgagg ggctcgatct gccgccggtc
     3241 gcgaaagcgc gcgccctgac cgaagcttga ccgttcgctg acgacttcgc gcggcgggcg
     3301 catcatcatc gccgcacgat gcaagtccgc ctcaccacgc cagggatgct gggcgcgctg
     3361 gtcgtcgcgc tggcggtatg gatcctgcac agcttcctgc cggcgctgct cgtcgcctgc
     3421 gtgaccgcgg tcgcaacctg gccgctctac cgccggtttg cggcactatt gcagccgcgc
     3481 atgtcgcggg gcgcgatttc tctcgccttc accgtcatgg tgttggcgct ggtgctggcg
     3541 ccactcatct ttgccggctc ggcgctgctc gccaaagcga gcgcgttgct cctcggcatg
     3601 gtcgccgccg acaaggccgg aatcggcgtt cccctgtggc tggagagcgt accgctcgcc
     3661 ggatcgtggc tcgccgagca ttggaggagc gagtttgcgc accccggagc gatccgcgaa
     3721 tgggtggagc agcgcgccga cccgt
//
LOCUS       DASRIE010000038         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1854766, whole genome shotgun sequence.
ACCESSION   DASRIE010000038 DASRIE010000000
VERSION     DASRIE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854766"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..723)
                     /locus_tag="VFB53_01705"
     CDS             complement(52..723)
                     /locus_tag="VFB53_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HZN85475.1"
                     /translation="MLLLIPGVLKQDQVDTVLGELGQSAYEDGRASAGLLAREVKNNL
                     QAKRDAEVVRKCGPMLLEALRRNGTFYSAALPHRVHGPMFNRYDVGMTYGLHVDNAIM
                     GGDQVAVRSDVSATLFLSAPNQYDGGELVIQENTGEKRIKLPQGSMVVYSSTSAHRVE
                     PVTRGSRLAAIFWVQSMVRDEPKREILFDLNQILAAVRDRLDSNEQMALTAVYHNLLR
                     TWAET"
     gene            764..1894
                     /locus_tag="VFB53_01710"
     CDS             764..1894
                     /locus_tag="VFB53_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85476.1"
                     /translation="MIALESKVLFAAALLCAASAQTWAQGVTPRTVALGQSAPLSGPA
                     RAHGEAIRNGALAYLRTLNDAGGLHGRRIELATLDDAGDPERALANSQRLVDDLRVFA
                     LFAYPAANLSRELLALVQKNRVPLFAPVSGAQIARQPARFVFTVRASHADEVERAVAH
                     YAQLGLKRFALVRRDDAEGTEIAEALRTALRRRGLAAAAVAALQPGKAAAAAQAALVA
                     DPEVVLIAAPAAPAAELLRSLKRARSPAQPLVLSQADPAILARSLGREGAGVALSQVV
                     PPLERISLPVVAEYRAAFEAETGGKDYSPASLEAYIAAKVFAEAVRRAGPALTREALL
                     LALEAMSAYDAGGYLVGYSRSNHQGSARTELLAIGRDGALLH"
     gene            complement(1891..>2858)
                     /gene="cysS"
                     /locus_tag="VFB53_01715"
     CDS             complement(1891..>2858)
                     /gene="cysS"
                     /locus_tag="VFB53_01715"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZN85477.1"
                     /translation="YRSAAGDVNYAVRKFPGYGRLSGKSLDELRAGERVEVDRSKQDP
                     LDFVLWKGAKPGEPTWPSPWGEGRPGWHIECSAMSCALLGEHFDIHGGGQDLQFPHHE
                     NEIAQSEGDSGRKFVNYWMHNGFVRVDEEKMSKSLGNFFLIRDILKRFDAEVVRFFIL
                     RAHYRSPLNHSDQNLDDAKQGLTRLYTALKGFDVDPGPIDWADRRAKRFRDAMDDDFN
                     TPEAVAVLFDLANEINKDRSEARARQLKSLAGILGLLQCDAQAFLQSAPAGALSDAQI
                     GERIAARLAARKSKDYAAADGIRRELEAAGVILEDGPAGTAWRRA"
BASE COUNT          398 a         1017 c          975 g          468 t
ORIGIN      
        1 agcagcgttt ctaaagttgt ggcagcagta tgcgagccgc cactagaacg ctcaggtctc
       61 cgcccaggtg cgcagcaggt tgtggtaaac cgccgtcagc gccatctgct cattgctgtc
      121 gagccggtcc cgcaccgctg ccaggatctg gttgaggtcg aacaggatct cccttttggg
      181 ctcgtcgcgc accatgctct gcacccagaa aatcgccgcc agccggctgc cgcgcgtcac
      241 cggttcgacc cggtgagcgc tggttgagga gtacaccacc atgctgccct gggggagctt
      301 gatgcgcttc tcgccggtgt tctcctggat caccagttcg ccgccgtcgt attggtttgg
      361 tgcgctgagg aacagggtcg ccgacacgtc ggagcgcacc gccacctgat cgccgcccat
      421 gatcgcgttg tcgacgtgca ggccgtaggt catgccgacg tcgtagcggt tgaacatcgg
      481 cccgtgcacc cggtgcggca gcgccgccga gtagaacgta ccgttgcgac gcagtgcctc
      541 gaggagcatc ggcccgcact tgcgcaccac ttcggcgtcg cgcttagcct gcaggttgtt
      601 cttcacttcg cgcgccagca ggccggcgct ggcgcggccg tcttcgtagg cgctctggcc
      661 gagctcgccc agcaccgtgt cgacctggtc ctgctttagc actccgggaa tcagcagcaa
      721 catgggcgcg cgtcggaccc gccaatcgtg gaaaattgcg gcgatgatag cactggagtc
      781 caaggtcctt ttcgctgccg ccctcctctg cgccgcaagc gcccagacct gggcgcaggg
      841 cgtcaccccg cgcaccgtcg cgctcggcca gtccgcgccg ctctcggggc ccgcgcgcgc
      901 gcacggcgag gcgatccgca acggcgcgct cgcctacctg cgcacgctca acgacgccgg
      961 cggcctgcac ggccgccgca tcgagctggc gacgctcgac gacgcgggcg atccggagcg
     1021 cgccctggcc aactcgcagc ggctggtgga cgacctgcgc gtattcgccc tgttcgccta
     1081 ccccgccgcc aacctgtcgc gcgagctgct cgccctggtg cagaaaaacc gcgtgccgct
     1141 gttcgccccg gtcagcggcg cgcagatcgc gcgccagcct gcgcgcttcg tattcaccgt
     1201 acgcgcgagc cacgccgacg aggtggagcg cgccgtggcg cattatgcgc agctcggcct
     1261 caagcgcttc gccctggtgc ggcgcgacga cgccgagggc accgagatcg ccgaggcgct
     1321 gcgcacggcg ttgcgccggc gcggcctggc ggcggccgcg gtcgcggcgc tgcagcccgg
     1381 caaggcagcc gccgcggcgc aggcggcgct ggtcgccgac ccggaggtgg tcctcatcgc
     1441 ggcgcccgcg gcgccggccg ccgagctgct gcgcagcctc aagcgcgcgc gcagcccggc
     1501 gcagcccctg gtgctgtcgc aggcggaccc tgcgatcctg gcgcgcagtc tcggccgcga
     1561 gggcgcgggc gtcgcgctgt cgcaggtggt gccgccgctc gagcgcattt ctctgccggt
     1621 ggtggcggaa taccgcgcgg cgttcgaagc cgaaaccggg ggcaaggact actcccccgc
     1681 ctccctcgag gcctacatcg cggcgaaggt gttcgccgag gcggtgcgcc gcgccggacc
     1741 ggcgctcacg cgcgaggcgc tgctgctggc gctcgaagcg atgtcagcct acgacgcggg
     1801 aggctacctg gtcgggtact cgcgcagcaa ccatcagggc agcgcgcgta ccgaactgct
     1861 ggccatcggc cgcgacggcg ccctgctgca ctaggcgcgg cgccaggcag tgccggcggg
     1921 cccgtcttcg agtatcacgc ccgccgcttc cagttccctg cggatgccat cggccgcggc
     1981 atagtccttc gacttgcgcg ccgcgaggcg cgccgcaatg cgctcgccga tctgcgcgtc
     2041 ggatagcgcg cccgccggcg ccgactgcag gaacgcctgc gcgtcgcact gcagcagccc
     2101 gaggattcct gcgagggact tcagctggcg cgcacgcgct tcggagcggt ctttgttgat
     2161 ctcgttcgca aggtcgaata gaacggcgac agcctcgggc gtattgaagt cgtcatccat
     2221 tgcgtcgcgg aaccgcttgg cgcggcggtc ggcccagtcg attggtccgg gatcgacgtc
     2281 gaatcccttg agcgcggtgt aaagccgcgt caggccttgc tttgcgtcgt ccaggttctg
     2341 gtccgagtga ttcagcggac tgcggtagtg cgcgcgcagg atgaagaagc gcaccacctc
     2401 ggcgtcgaaa cgcttgagga tgtcccggat caggaagaag ttgcccagcg acttggacat
     2461 cttctcctcg tcgacgcgca cgaagccgtt gtgcatccag taattgacga acttgcgccc
     2521 gctgtcgcct tcggactggg cgatctcgtt ctcgtggtgg gggaactgca gatcctggcc
     2581 gccgccgtgg atgtcgaagt gctcgccgag cagcgcgcag ctcatcgccg agcactcgat
     2641 gtgccagccg gggcgcccct cgccccaggg cgacggccat gtcggctcgc ctggcttggc
     2701 gcccttccac agcacgaagt cgagcgggtc ctgcttgctg cggtcgacct cgacgcgctc
     2761 cccggcgcgc agctcgtcca gcgatttgcc ggacagtctt ccgtagccgg ggaacttgcg
     2821 caccgcgtaa ttcacgtcgc ccgccgcgga gcggtacg
//
LOCUS       DASRIE010000039         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2379764, whole genome shotgun sequence.
ACCESSION   DASRIE010000039 DASRIE010000000
VERSION     DASRIE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379764"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFB53_01720"
     CDS             complement(<1..899)
                     /locus_tag="VFB53_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN85478.1"
                     /translation="MNPSSTAPATVAPAVPADASHGADPQRVLADRVEQLYSQMPLGI
                     VVTLAIGGIATYELWDARTRDLVLFWWGLVLLVTAARAALYFGYRSSTGKLAEAAQWL
                     RWLGISALANGVNWGFAGAVFFPSHTDEQQVFLAFLLAGIASAGIPTYAASWPMFALY
                     AGAIVLPFAYVLATFGNRLFAEIALLVPVFYLVNVSIAYRLNQVFYSGYRLRHAYGRL
                     TEDYTALNQRLEQQLVELDEARRQVEASGRKLALFAERAPIAVLELDGEGRIQRINQT
                     DENLFGWTSNELVEKNTSVKFLVR"
     gene            complement(969..1604)
                     /gene="lexA"
                     /locus_tag="VFB53_01725"
     CDS             complement(969..1604)
                     /gene="lexA"
                     /locus_tag="VFB53_01725"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZN85479.1"
                     /translation="MSLPEPLTDRQSEILKLIRDLTESSGFPPTRAEIAEKMGFRSVN
                     AAEQHLRALERKGALDILQGASRGLRVRDKAPARVGRLMELPVVGRVAAGSPMLAEEH
                     LQGRFQVDTNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHRTPEARTGQVVVARLAD
                     EVTVKRFRRRGHSVQLLPENPAFEPIEIDLRRDPLTIEGVAVGVIRNGKGL"
     gene            1696..2553
                     /locus_tag="VFB53_01730"
     CDS             1696..2553
                     /locus_tag="VFB53_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HZN85480.1"
                     /translation="MAAILVHGGAGRWAAEDRTLAVAGVARAAAVGWAVLAGGGSALD
                     AAVEAVVALEDDPLFNAGTGSVLNADGEVEMDASVMAGDTLAAGGVACIRRVRNPVLV
                     ARRVLQATDCVLLAGAAATAFAREQGFADHDPGLPRALRKAAGVGTVGAVALDAAGRL
                     AAATSTGGREGKRAGRVGDAPIPGAGNFACAQAAVSATGWGEQMLRYVTARDVAGRIE
                     RGAAPAEALRLALDDMARRFGEPIGAIALDARGNLGAVHGTEAMPHAWRRSGEPGVTA
                     QFAAGRIPA"
BASE COUNT          359 a          900 c          992 g          374 t
ORIGIN      
        1 cgcaccagga acttcaccga ggtgttcttc tccaccagct cgttggaggt ccagccgaac
       61 aggttctcgt cggtctggtt gatcctctgg atgcggccct cgccgtccaa ctcgagcacg
      121 gcgatcggcg cgcgctcggc gaacagcgcg agcttcctgc ccgaggcctc gacctggcgc
      181 cgcgcctcgt cgagttccac cagctgctgc tcgagccgct ggttgagcgc ggtgtagtcc
      241 tcggtcagcc ggccgtaggc gtggcgcagg cggtagccgg agtagaacac ctggttcagc
      301 cggtaggcga tgctgacgtt gaccaggtag aacacgggca ccagcagcgc gatttccgcg
      361 aacaggcggt tgccgaaggt ggccagcacg taggcgaagg gcaggacgat cgcgccggcg
      421 tacagcgcga acatcggcca ggaggcggcg taggtcggaa tccccgccga ggcgatgccg
      481 gccagcagga aagccaggaa tacctgctgc tcgtcggtgt gcgacgggaa gaacaccgcg
      541 ccggcgaagc cccagttcac gccgttggcg agggcgctga tgccgagcca gcgcagccac
      601 tgcgcggcct cggccagctt gccggtgctg gagcggtagc cgaaatacag ggcggcgcgc
      661 gccgcggtca ccagcagcac caggccccac cagaacagca ccaggtcgcg ggtacgcgcg
      721 tcccacagct cgtaggtggc gatgccgccg atggccaggg tcaccacgat gccgagcggc
      781 atctggctgt agagctgctc gacgcggtcg gcgaggacgc gctgggggtc ggcgccgtgg
      841 ctcgcgtccg cgggaactgc gggcgcgacg gttgcgggcg ctgtgctgct gggattcatg
      901 ccggggcggg ggggtccgcc ccgattatat cggtacgtcc ggcgcgcgcc gggccgattg
      961 cgcgagggct acagcccctt gccgttgcgg atcacgccga ctgcgacccc ctcgatggtc
     1021 agcggatcgc ggcgcaggtc gatctcgatg ggctcgaacg cgggattctc cggcagcagc
     1081 tgcacgctgt ggccgcgccg gcggaagcgc ttgaccgtga cctcgtcggc caggcgcgcc
     1141 accaccacct ggccggtgcg cgcctcgggc gtgcggtgca ccgccagcag gtcgccttcc
     1201 aggatcccgg cgtcgcgcat cgacatgccg cgcacccgca gcaggtaatc ggcgcgcggc
     1261 gtgaacaggt tcgtatccac ctggaagcgc ccctgcaggt gctcctcggc cagcatcggg
     1321 ctgccggccg caacgcggcc cacgaccggc agctccatca ggcggccgac ccgcgccggt
     1381 gccttgtcgc gcacgcgcag gccgcgcgag gcgccctgca gaatgtcgag ggcgcctttt
     1441 ctttccagcg cgcgcaagtg ctgctcggcg gcgttcaccg agcggaagcc catcttctcg
     1501 gcgatttccg cgcgggttgg cggaaagccg gaagactcgg tgaggtcgcg gatcagcttc
     1561 aggatctcgc tctggcggtc ggtcagaggt tcgggcaggg acatgcgcgg tctccttggg
     1621 tcctggacct actgtcggtc cttacagtgc tggcattata tccagtatca gcgggcgcgc
     1681 aacgtagaat ggcccatggc tgcaatcctg gtgcacgggg gtgcggggcg ctgggccgcg
     1741 gaggaccgga ccctcgccgt ggcgggcgtg gcgcgcgccg cggcggtcgg ctgggcggtg
     1801 ctggcgggcg gcggcagcgc gctcgacgcc gccgtcgagg cggtggtggc cctcgaggac
     1861 gacccgctgt tcaacgccgg caccggctcg gtgctgaacg ccgacggcga agtcgagatg
     1921 gacgccagcg tgatggcggg cgacacgctg gccgccggcg gcgtggcctg catccgccgc
     1981 gtgcgcaatc cggtgctggt cgcgcggcgc gtgctgcagg ccaccgattg cgtgctgctg
     2041 gcgggggcgg cggccactgc gttcgcgcgc gaacagggat tcgccgacca tgacccgggc
     2101 ctgccgcgcg ccttgcgcaa ggcggcgggc gtcggcacgg tgggcgcggt ggcgctggac
     2161 gcggcgggcc ggctcgccgc ggcgacctcg accggcggcc gcgagggcaa gcgcgcgggg
     2221 cgcgtcggcg atgcgccgat tccgggcgcc ggcaactttg cctgcgcgca ggcggcggtg
     2281 tcggccaccg gctggggcga gcagatgctg cgctacgtca ccgcccggga cgtcgccggg
     2341 cgcatcgagc gcggcgcggc gccggccgag gccttgcgcc tggcgctcga cgacatggcg
     2401 cggcgcttcg gcgagccgat cggcgccatc gcgctcgacg cgcgcggcaa cctgggcgcg
     2461 gtgcacggta ccgaggccat gccgcacgcc tggcgccgca gcggcgaacc gggcgtcacc
     2521 gcgcagttcg ccgccggtag aatccccgca tgatcgtccg ctcgctgctc gacaccgacc
     2581 tgtacaagtt caccatgatg caggtggtgc tgcaccattt cccgg
//
LOCUS       DASRIE010000040         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3162631, whole genome shotgun sequence.
ACCESSION   DASRIE010000040 DASRIE010000000
VERSION     DASRIE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162631"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..770)
                     /locus_tag="VFB53_01735"
     CDS             complement(6..770)
                     /locus_tag="VFB53_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85481.1"
                     /translation="MLRVDGVGVRFGGIVALDGVSFDVEAGRIVGLIGPNGAGKTTLF
                     NCLSRLYECGTGDILFNGVSLLQSPKHGIAALGIGRTFQNLALFRTMTVLDNIMVGHH
                     CRTQSGFLFNALRLPLVAHEEHATLEKAKSLIRFLALDAVAGARVADLPFGTQKRVEL
                     ARALAAEPKLLLLDEPASGLNHEEVGELGVLIREIRDRLGLTVLLVEHHMSLVMSLSD
                     HIVVLNFGRKIAEGTPAQIQNHPDVIEAYLGAGANV"
     gene            895..1665
                     /locus_tag="VFB53_01740"
     CDS             895..1665
                     /locus_tag="VFB53_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN85482.1"
                     /translation="MSDLAEKFGRIHSIPAYQLVAEAIENRILAGGIRPGEPIGTEAE
                     LVKQFGVNRSTVREGIRLLEHDGIIQRQSNRRLAVSLPHYERLASRTTRAMVLHEVTF
                     RELHEAAMALQLATIEGAAARATPALVAALEDNVERTARALGDADAVAGLDAEFHALI
                     GRASANRVLQLAREPSDLLVYPTTRMILAKVKEGPARLLEAHRMLLDAIRRGDAAAGR
                     LWARRHLNDWRKGFERAGNDIDQPIDRLYLREALTSSS"
     gene            complement(1649..2629)
                     /locus_tag="VFB53_01745"
     CDS             complement(1649..2629)
                     /locus_tag="VFB53_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85483.1"
                     /translation="MRKLLAAALAAALAAPAAAQTKLDFILNWVPGGDHAPYYYAKAQ
                     GWYAREGIDLNLEPGKGSMLAVQRVGAGANPIGLADMGNVLIAKGKGADTVGVFNVYA
                     NSPQGLYWLKSSGIKGIKDFAGKKIGNPAADGARPMWPALAKANGVDPKSVTWVNVDA
                     NAKLSALKSKAIDVTTSFYNIHHIFQRELGTDMGFVAWRDVGLNPYGNSVVVNAAFLQ
                     KNRPLVGKFVRVTQRAFAACVQDPKPCVQALVDANGALKFDNEMTNWSLVEVLMSDKY
                     SKSVALGIHDDARMQADYALVRDYVGIDKPFDVKTSYTNEFLDRTVRMTK"
     gene            2729..3781
                     /locus_tag="VFB53_01750"
     CDS             2729..3781
                     /locus_tag="VFB53_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN85484.1"
                     /translation="MPLHRSSLPLETVALLRRGTVIPAHPLALDARRRLDARRQRALT
                     RYYLDAGAGGLAVGVHATQFAIREQGLYEPVLKLAADTARDWAKRPLVMIAGLSGATA
                     QAQQEARIALAHGYHAGMLSLAPLRGATLDELVEHCAAVAAEIPLVGFYLQTAVGGIA
                     LPMEFWRRFALIDNVVAIKAAPFDRYRTLDVVRGVVAAQAEERVALYTGNDDHIVLDL
                     AVPFAVRRGGREVRVRIKGGLLGHWSVWTKSAVELLERIQRAPRLDEDLLALDARVTD
                     CNSAFFDAANGFAGCIAGCHEVLRRQGLLEGIWCLDPKETLSPGQAEEIERVCREHAD
                     LADDAFVRANLGLWLS"
     gene            3795..>4028
                     /locus_tag="VFB53_01755"
     CDS             3795..>4028
                     /locus_tag="VFB53_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85485.1"
                     /translation="MNETLQRYALAVLAHAALLLAWYLFVKLGNVPTFVMPSPGATLD
                     ALLQPNYDWWANMLVTGTEIFAGYLLALVAGVGL"
BASE COUNT          582 a         1393 c         1411 g          642 t
ORIGIN      
        1 gcaggttaga cattcgcccc ggctccgagg taggcctcga tgacgtccgg atggttctgg
       61 atctgcgccg gcgtgccctc ggcgatcttg cggccgaaat ttagcaccac gatgtgatcc
      121 gacaggctca tcaccaggct catgtggtgc tcgaccagca gcaccgtgag gcccaggcgg
      181 tcgcggatct cgcggatcag cacgcccagt tcgcccacct cctcgtggtt caggccgctc
      241 gccggctcgt cgagcagcag gagcttgggc tcggccgcca gggcgcgcgc cagctccacg
      301 cgcttttgcg tgccgaacgg caggtcggcg acgcgcgcgc cggcgacggc gtccagcgcg
      361 aggaagcgga tcagcgactt cgccttctcc agcgtggcat gctcctcgtg cgccaccagc
      421 ggcaggcgca gcgcgttgaa caggaagccg ctctgcgtgc ggcagtggtg cccgaccatg
      481 atgttgtcca gcacggtcat ggtgcggaac agggcgaggt tctggaaggt gcgcccgatg
      541 ccgagcgcgg cgatgccgtg cttgggcgac tgcagcagcg acacgccgtt gaacaggatg
      601 tcgccggtgc cgcattcgta gaggcgcgac aggcagttga acagcgtggt cttgcccgcg
      661 ccattgggcc cgatcaggcc gacgatgcgc ccggcctcga cgtcgaagga aacgccgtcc
      721 agcgcgacga tgccgccgaa gcgcaccccg acgccgtcca cgcgcagcaa cgcccctgtc
      781 gcaaccggtt cgatcgctcc tcctccaaag gaatcgtccc acagcgccgc ccgggcgcta
      841 ttgcttattg tgtcggattg tcggacgata ttttgcccgg agtcaagaaa agagatgagc
      901 gacctcgccg aaaagttcgg gcggatccat tccatcccgg cctatcagct ggtggcggaa
      961 gccatcgaga accgcatcct cgcgggcggc atccgcccgg gcgagccgat cggcaccgag
     1021 gccgagctgg tcaagcagtt cggcgtcaac cgctccacgg tgcgcgaagg catccggctc
     1081 ctcgagcacg acggcatcat ccagcgccag tccaaccggc gcctggcggt cagcctgccg
     1141 cactacgagc ggctggcgag ccgcaccacg cgcgccatgg tgctgcacga ggtgaccttc
     1201 cgcgaactcc acgaggcggc gatggcgctg cagctcgcca ccatcgaggg cgccgcggcg
     1261 cgcgccacgc ccgccctggt ggcggcgctc gaggacaacg tcgagcgcac cgcgcgcgcg
     1321 ctgggcgacg cggacgcggt cgccggcctg gatgcggagt tccacgcgct catcggcagg
     1381 gcctcggcga accgcgtgct gcagctggcg cgcgagcctt ccgacctgct ggtctacccg
     1441 acgacccgca tgatcctcgc caaggtgaag gagggaccgg cgcggctgct ggaagcgcac
     1501 cgcatgctgc tcgatgcgat ccgccgcggc gacgccgcgg ccggccggct gtgggcgcgg
     1561 cgccacctga acgactggcg caagggtttc gagcgcgccg gcaacgacat cgaccagccc
     1621 atcgaccggc tctacctgag ggaagccctc acttcgtcat cctgaccgtg cggtcgagga
     1681 attcgttggt gtaggaggtc ttgacgtcga agggcttgtc gatgccgacg tagtcgcgca
     1741 ccagcgcgta gtcggcctgc atgcgggcgt cgtcgtggat gccgagcgcg acgctcttgg
     1801 agtacttgtc gctcatcagc acttccacca ggctccagtt ggtcatctcg ttgtcgaact
     1861 tgagcgcgcc gttggcgtcg accagcgcct gcacgcaggg cttggggtcc tgcacgcagg
     1921 cggcgaaggc gcgctgcgtg acgcgcacga acttgcccac caggggccgg ttcttctgca
     1981 ggaaggcggc gttcaccacc accgagttgc cgtaggggtt gaggccgacg tcgcgccagg
     2041 cgacgaagcc catgtcggtg cccagctcgc gctggaagat gtggtggatg ttgtagaacg
     2101 aggtggtgac gtcgatggcc ttcgacttga gcgccgagag cttggcgttg gcgtccacgt
     2161 tcacccaggt gaccgacttc gggtcgacgc cgttcgcctt ggccagcgcc ggccacatcg
     2221 gccgcgcgcc gtcggccgcc gggttgccga tcttcttgcc ggcgaagtcc ttgatgccct
     2281 tgatgcccga gctcttcagc cagtacaggc cctgcggcga gttggcgtag acgttgaaca
     2341 cgcccaccgt gtccgcgccc ttgcccttgg cgatgagcac gttgcccatg tcggcgaggc
     2401 cgatcgggtt ggcgccggcg ccgaccctct gcaccgcgag catcgagccc ttgcccggct
     2461 ccaggttgag atcgatgcct tccctggcgt accagccctg cgccttggcg tagtagtagg
     2521 gcgcgtggtc gccgccgggg acccagttga ggatgaaatc gagcttggtc tgggccgccg
     2581 cgggcgcggc gagcgcggcg gccagcgccg cggcgagcaa tttgcgcatg acttcctcct
     2641 gtggaacctg gtctgcggcg atggtatcgg cgcggtttcg cagcgtcaat ggctggacgc
     2701 ggttaaccgc ccggttacaa tttcccgcat gccgctgcac cgctcgtcgc tgccgctgga
     2761 gaccgttgcg ctgctgcgcc ggggcacggt gatcccggcg catccgctcg cgctcgatgc
     2821 gcgccgccgc ctcgatgcgc gccgccagcg cgcgctgacg cgctactacc tcgatgcggg
     2881 cgcgggcggg ctggcggtgg gcgtgcacgc cacgcagttc gccatccgcg agcagggcct
     2941 gtacgagccg gtgctcaagc tcgccgccga caccgcgcgc gactgggcca agcgcccgct
     3001 ggtgatgatc gccggcctgt cgggcgccac cgcgcaggcg cagcaggaag cgcgcatcgc
     3061 gctggcgcac ggctaccacg ccggcatgct gtcgctcgcg ccgctgcggg gcgcgacgct
     3121 ggacgagctg gtggagcact gcgcggcggt cgccgccgag atcccgctgg tcggcttcta
     3181 cctgcagacc gcggtcggcg gcatcgcgct gccgatggag ttctggcgcc gcttcgcgct
     3241 catcgacaac gtggtggcga tcaaggccgc gcccttcgac cgctaccgca cgctcgacgt
     3301 ggtgcgcggc gtggtggcgg cgcaggccga ggagcgcgtc gcgctgtaca ccggcaacga
     3361 cgaccacatc gtgctcgacc tggcggtgcc cttcgccgtg cggcgcggcg gccgggaggt
     3421 gcgcgtgcgc atcaagggcg ggctgctcgg gcactggagc gtgtggacca agagcgccgt
     3481 cgagctgctc gagcgcatcc agcgcgcgcc gcgcctcgac gaggacctgc tcgcgctcga
     3541 tgcgcgcgtc accgactgca acagcgcctt cttcgacgcg gccaacggtt ttgccggctg
     3601 catcgccggc tgccacgagg tgctcaggcg ccagggcctg ctggagggca tctggtgcct
     3661 ggacccgaag gaaaccctgt ctcccggcca ggccgaggag atcgagcgcg tgtgccgaga
     3721 gcacgccgac cttgccgacg acgccttcgt gcgcgctaat ctcgggctgt ggctgtcctg
     3781 accgcgaggc tccgatgaac gagaccctgc aacgctacgc gctggcggtg ctggcgcacg
     3841 ccgccctgct gctcgcctgg tacctgttcg tcaagctggg caacgtgccg acattcgtca
     3901 tgccctcgcc cggcgcgacg ctggacgcgc tgctccagcc gaactacgac tggtgggcca
     3961 acatgctggt caccggcacc gagatcttcg cgggctacct gctcgcgctg gtggcgggcg
     4021 tggggctg
//
LOCUS       DASRIE010000041         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1096289, whole genome shotgun sequence.
ACCESSION   DASRIE010000041 DASRIE010000000
VERSION     DASRIE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096289"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFB53_01760"
     CDS             <1..327
                     /locus_tag="VFB53_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HZN85486.1"
                     /translation="DLSAAAPVAGITGLAVLPAGPLPPNPLELLSRGAFAALLSRAQA
                     EYDVILIDTPAVTQYADASAITFRSGDALMISRKDHTRLADTEQAMRDLSDASARVVG
                     TLMNSY"
     gene            441..1883
                     /locus_tag="VFB53_01765"
     CDS             441..1883
                     /locus_tag="VFB53_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN85487.1"
                     /translation="MATLTLPQARAQAPARHLWLIAAFVLMVGAIAGGYALATAELQA
                     LYIGLAGAAAVAVLLDYRIGAILLVLLLPVSATSLFPRGLLGVTGLNPVNMLLGATLI
                     AYLAKGRLEHAGAFIPRPLLWLYIVPIVAAGLLGIRHVDDIHPGFYDALVINFTTWPG
                     YLRDNMVKPLLTVLIAVLVGAAVAKARKPEPYVTALMVGVVLLALVMFSFVIASGVRI
                     GWLASPRARAFFDQIGTHANELGRTFVTTYALLLFAWWETKHEKAKVAMFAALGIITI
                     GIVLTFSRNAFLGFFLVNGLFLLWRFQAKKLGLAMLGFAAAAAFAPNAVYRRLTHNLD
                     TGNADAVSAGRIEGIWGPLIPDMMESPIWGQGLDSILWSNAVSSGHALFVGHPHNAYL
                     QAVLDMGVIGLALLVAYYFTVWRGFRALGSNAYLSPTMRGFFQGACAALLAFALAGVT
                     GGSLRPQPENVPLWMAIGIMYGLLARRPAS"
BASE COUNT          282 a          717 c          699 g          349 t
ORIGIN      
        1 gacctgtcgg ccgcggcgcc ggtggccggg attaccggcc tcgcggtgct gcccgccggg
       61 ccgctgccgc ccaatccgct cgagctgctg tcgcgcgggg cctttgccgc gctgctgtcg
      121 cgcgcgcagg ccgagtacga cgtgatcctg atcgacacgc cggcggtcac gcagtacgcc
      181 gacgcctcgg cgatcacctt ccgttcgggc gacgcgctga tgatctcgcg caaggaccac
      241 acgcggctcg cggacaccga gcaggcgatg cgcgacctga gcgacgccag cgcccgcgtg
      301 gtgggcacgc tgatgaactc gtactaggaa gcgatgaaga aaccgaagct cctgctggcg
      361 gtgctcgcgc tcctgatcgg ggcggcgagc gctgccggca cgaagtttcc cgcaactgaa
      421 catcaagggg atctgctgac atggcgacgc tgacgttgcc gcaggctcgt gcgcaggcac
      481 cggcccgcca cctctggctg atcgcggcgt tcgtgctgat ggtcggtgcg atcgccggcg
      541 gctacgccct cgcgacggcg gagctccagg cgctctacat cggcctggcg ggcgccgcgg
      601 cggtcgcggt gctgctcgat taccgcatcg gcgcgatcct gctggtgctg ctgctgccgg
      661 tgtccgccac ctcgctgttt ccgcgcggcc tgctgggggt caccggcctg aacccggtca
      721 acatgctgct cggcgcgacg ctgatcgcct acctggcgaa ggggcgcctc gagcacgccg
      781 gcgcgttcat cccgcggccg ctgctgtggt tgtatatcgt gccgatcgtg gccgccggcc
      841 tgctcggcat ccgccacgtc gacgatatcc accccggctt ctacgacgcg ctggtgatca
      901 acttcaccac ctggcccggc tacctgcgcg acaacatggt gaagccgctg ctgacggtgc
      961 tgatcgcggt cctggtgggc gcggcggtgg cgaaggcgcg caagccggag ccctacgtga
     1021 cggcgctgat ggtcggcgtg gtgctgctcg cgctggtgat gttcagcttc gtcatcgcga
     1081 gcggcgtgcg catcggctgg ctcgcgtcgc cgcgcgcgcg cgcgttcttc gaccagatcg
     1141 gcacgcacgc caacgagctc ggccgcacct tcgtcaccac gtacgccctg ctgctgttcg
     1201 cctggtggga gaccaagcac gagaaggcca aggtcgcgat gttcgccgcg ctcggcatca
     1261 tcaccatcgg catcgtgctg acgttctcgc gcaacgcgtt cctcggcttc ttcctggtga
     1321 acggcctgtt cctgctgtgg cgcttccagg ccaagaagct cggcctggcg atgctcggct
     1381 tcgcggcggc cgcggcgttc gcgccgaacg cggtctaccg gcggctgacc cacaacctcg
     1441 ataccggcaa cgcggacgcg gtgtccgccg gccgcatcga aggcatctgg ggaccgctga
     1501 tccccgacat gatggaaagc ccgatctggg gccagggcct cgactcgatc ctgtggtcga
     1561 acgcggtcag cagcggccac gcgctgttcg tcggccaccc gcacaacgcc tacctgcagg
     1621 cggtgctcga catgggcgtg atcggcctcg cgctgctggt cgcgtactac ttcaccgtgt
     1681 ggcggggctt ccgcgcgctc ggcagcaatg cctacctttc tccgacgatg cgcggcttct
     1741 tccagggcgc gtgcgcggcg ctgctcgcgt tcgcgctcgc cggcgtcacc ggcggcagcc
     1801 tgcggccgca gccggagaac gtgccgctat ggatggcaat cggcatcatg tacgggctgc
     1861 tcgcccggag gccggccagc tagcaatggg cttcgccgag aaggttcctg cgggcttcaa
     1921 accgacagcc cgcgccgctg tgttgcctgc aacaaaacgg ttgcaggaga aaaagcacat
     1981 ctgcttcgta gcgccgcacg cctggcccgt cctgtcgcgc gacaagcaca tcaaggtgat
     2041 gggcggg
//
LOCUS       DASRIE010000042        14364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_814790, whole genome shotgun sequence.
ACCESSION   DASRIE010000042 DASRIE010000000
VERSION     DASRIE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814790"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1010)
                     /gene="lipA"
                     /locus_tag="VFB53_01770"
     CDS             complement(69..1010)
                     /gene="lipA"
                     /locus_tag="VFB53_01770"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZN85488.1"
                     /translation="MREAGVKEKGREKTARIPIKIVPRSPLAKPSWIRVKAASPSTRF
                     YEIKQILREQKLHTVCEEASCPNIGECFGKGTATFMILGDICTRRCPFCDVGHGRPLP
                     VDAEEPGHLAGTIAKLKLKYVVITSVDRDDLKDGGAQHFVDCIRAVREKSPATTIEVL
                     VPDFRGRLDRALGILLAAPPDVMNHNLETVPRLYRRARPGGDYLHSLRLLQEFKKSVP
                     SVPTKSGLMVGLGETDEEILQVMRDLRAHQVDMLTIGQYLQPSPHHLPVERYVHPETF
                     AMFEGEAGAMEFRHAAIGAMVRSSYHADKQAEEAGVA"
     gene            complement(1003..1629)
                     /gene="lipB"
                     /locus_tag="VFB53_01775"
     CDS             complement(1003..1629)
                     /gene="lipB"
                     /locus_tag="VFB53_01775"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZN85489.1"
                     /translation="MDVALAPRIKSLGLTDYVAVLARMREFTAHRDTETPDELWVVEH
                     PPVYTLGQGADAKFGPKTANGIPVVQAERGGEITYHGPGQAVIYTMVDIARRGIKVKT
                     YVQMLEQCVINFLISYEIPAERKAGAPGVYVGGAKIASLGIRVTRGRAWHGLALNVDM
                     DLAPFAAIDPCGYPGLSVVQARDLGVMGTAKEVGIRLARLIAHRLEHA"
     gene            complement(1629..1901)
                     /locus_tag="VFB53_01780"
     CDS             complement(1629..1901)
                     /locus_tag="VFB53_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HZN85490.1"
                     /translation="MPIANEESLVSYPTDFPIKVMGRKQARFAHTVVSIVRKHAPDFD
                     AATMELRPSRQGTYLSVTCTIRAQSREQLDALYQDLCDHPAVVMVL"
     gene            complement(1901..2761)
                     /locus_tag="VFB53_01785"
     CDS             complement(1901..2761)
                     /locus_tag="VFB53_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HZN85491.1"
                     /translation="MVFLNGKFLPIEQAMVPVLDRGFIFGDGVYELIPVYSRAPFRMD
                     EHLARLERSLAAVRIRNPYPREQWREIILKLVAQQPWQDQAVYFQVTRGVAKRDHAFP
                     AGVEPTVFVMCNPLVNPPKELVEKGAAAVSAVDNRWLRCDIKSISLVGNCLLRQASAD
                     AGAAETILFREGKLTEASASNVFVAKNGLLLSPPKSNLILPGITYDVVVEIAQATKIP
                     LEFRDVSEAEVRAADEIWLTSSSKEILAVATLDGKPVGDGKPGPLFRKAYAGYQEFKA
                     KVMRKEAVSA"
     gene            complement(2772..3899)
                     /locus_tag="VFB53_01790"
     CDS             complement(2772..3899)
                     /locus_tag="VFB53_01790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZN85492.1"
                     /translation="MRAGPVAGVLLLACTAATAAPPVAPQLAARAWVLADLSSGQVLA
                     AERAGERFAIASLTKLMSAYVVFGALRDKRLEPAAQVQVSQRAWRAGGSRMFIEPGRA
                     VSVEELIRGVVVQSGNDAALALAESLAGSEDAFVALMNREAERLGMRQTSFANATGAP
                     APGHASTAGDLALLAGALIRDFPADYARYYAQKEFSYNGITQQNRNRLLWLDPSVDGV
                     KTGHTEEAGYCLIASSARGGRRLISVLLGASTEGARARESQKLLNWGYQNFDGVKLYA
                     PGEAVKSIAVWKGAAALVPAGFAQGLVVTVPKGEAALLKAELATLPPVLAPVAKGQRV
                     GTLRVALGGAPLGEYPVTALAAVPAAGFFGRAWDTLRLWFE"
     gene            complement(3912..4448)
                     /locus_tag="VFB53_01795"
     CDS             complement(3912..4448)
                     /locus_tag="VFB53_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZN85493.1"
                     /translation="MRRIALALLLAAGCSSTPDKRPGGYYKDDGPGDSAKLASIPDAV
                     PRHEPLHRYANRPYEALGKRYVPLTRVGPFEQRGAASWYGKRFHGKPTSSGEAYDMYK
                     MSAAHPILPIPSYARVTNLANGSKVVVRINDRGPFHDGRVIDLSYAAAHRLGYIAAGS
                     ARVLVEQVVPGGAPLALR"
     gene            complement(4445..5704)
                     /locus_tag="VFB53_01800"
     CDS             complement(4445..5704)
                     /locus_tag="VFB53_01800"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZN85494.1"
                     /translation="MKLLVKGGRVVDPASGRDAVGDVFIADGKIAAAPGTPDRTLDAK
                     GLVVAPGLVDLAARLREPGYEHKATLESEMDAAVAGGVTSLACPPDTDPPLDEPGLVD
                     MLRRRAKALSRARVYPVGALTVKLAGRQLTEMAELAEAGCAAFSQGNAAVEDTQVLWR
                     ALQYAATFGLPVWLRPEDAWLARGGVAHDGEVATRLGLAGIPAFAETIALAAIFELVR
                     ATGARVHLCRISSAAGVALLRRAKHDGLPITCDVGIHHVHLSDMDLGYFDAQCRLEPP
                     LRSQRDREALAAGLADGTIDCAVSDHAPVDEDAKHLPFAEAEPGATGLELLLPLTLKW
                     GMERKLTLAQTLARISSAPARILGAASGRLEPGAPGDLVLFDPQASWRVTPEALKSQG
                     KNSPFLGMELAGRVRATVVAGQVVYEA"
     gene            complement(5701..6654)
                     /locus_tag="VFB53_01805"
     CDS             complement(5701..6654)
                     /locus_tag="VFB53_01805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZN85495.1"
                     /translation="MPGNPQLDANGELQHLLSIEGLPRELILKILDTAESFAGVTERD
                     IKKVPLLRGKSVFNLFFEGSTRTRTTFEIAAKRLSADVINLNVAVSSTAKGESLLDTV
                     ANLSAMQADIFVVRHGSSGAAHLVARHVKPHEHVINAGDGRHAHPTQGLLDLYTIRHY
                     KKDFSGLTVAIVGDVLHSRVARSLIHGLTTLGAPEVRVIGPQTLIPAGAAALGVRVFH
                     SMREGLRGADVVVMLRLQNERMTGALLPSAQEFNKHYGLTAEKLALAKPDAIVMHPGP
                     MNRGVEIDSAVADGPRSVILPQVTFGIAVRMAVMSMVAGGG"
     gene            complement(6644..7144)
                     /gene="pyrR"
                     /locus_tag="VFB53_01810"
     CDS             complement(6644..7144)
                     /gene="pyrR"
                     /locus_tag="VFB53_01810"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZN85496.1"
                     /translation="MSKLPDAEKLCTQLARELKARVTPASAMVGIHTGGAWVAERLHA
                     ALGIKEPLGLLDPAFYRDDYAKQGLARDPKRSKIPFAVEGRDLLLVDDVLYTGRTVRA
                     AMNELFDYGRPASIVLVVLAERDGRQLPVCAQLAGAKVEVAAGMRLRLRRDGAGRLSL
                     ELERAG"
     gene            complement(7128..7520)
                     /gene="ruvX"
                     /locus_tag="VFB53_01815"
     CDS             complement(7128..7520)
                     /gene="ruvX"
                     /locus_tag="VFB53_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZN85497.1"
                     /translation="MPEAGTVLAFDFGIKRIGVAVGEAELGTAHPLPGIAAPGFAAIG
                     KLVKEWRPAALVVGLPVAEGGEHPLARRVQRFARQLEGRFRLPVAQVDERYTSVAAES
                     LLRGRKRKPVDSVAAQLILEQYFHEQAA"
     gene            complement(7513..8034)
                     /locus_tag="VFB53_01820"
     CDS             complement(7513..8034)
                     /locus_tag="VFB53_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZN85498.1"
                     /translation="MPSMADPYFAKSLTYVCEHNAQGALGVVINRPIDMTLQALFERL
                     SLTLKHKDLSDAPIYFGGPVQTDRGFVLHEPAGSWQSTLKVRDAIGLTTSKDILEAVG
                     RGEGPQKILVTLGYAGWSAGQLEHELGQNAWLTVEAKDAIIFDLPSEERLPAAMELLG
                     VDYARLADSAGHA"
     gene            complement(8115..9908)
                     /locus_tag="VFB53_01825"
     CDS             complement(8115..9908)
                     /locus_tag="VFB53_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HZN85499.1"
                     /translation="MDLKTLGAAWRGVAELPLRRQLALLAAATAALLAAALWLAWAEY
                     REAHFGAGHVAAAGELRMLSQRLAKASLRSLRGEPEAFAQIQASRDGFAAGLALLARE
                     DRAPQALRALERDWERTERNAATLLRDQKLLTALATAAAGMERGNPRLLERAERVQNL
                     KLARGAAAAEVAAASRLVMLTQRMARGAQQLLAADGVEPEVEFLLGKDVNEFRGTLER
                     LRAGARDEETAAALAALGETFAGYQDAVVAVLGTRARLGGAKAAALAVYDDAERLLQS
                     VDGLVAATRDAPASRAYEAGLAAIVLALAGALALAALAFAHDERRARDSATRRLGVLR
                     RYHERDLEAVEKLRRELEGLATGDLTARASVGSEITQPAAAALNDALDALRALVARVA
                     VAAERVGGAAGSAQAAADGLLDSARLQSERIKGASARALGMARAVTDLSARSNQSAEM
                     ARAALAAATQGRQAVQAALSVVEEIGRRVRLAAQSVDRVRGASPEMAKTAQLLSQIAA
                     QAEALGGSASGRTLAELGELLRELADRIEQVSKTAQSQGAAASAIAGGVREMLSGAEQ
                     AASAATGAAASVEEVAGLLRELQEALEKLRY"
     gene            complement(9925..10110)
                     /locus_tag="VFB53_01830"
     CDS             complement(9925..10110)
                     /locus_tag="VFB53_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZN85500.1"
                     /translation="MTENQVQFKTWMCLICGFVYDEAAGMPDEGLAPGTRWEDVPINW
                     TCPECGARKEDFEMVEI"
     gene            10186..10932
                     /locus_tag="VFB53_01835"
     CDS             10186..10932
                     /locus_tag="VFB53_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HZN85501.1"
                     /translation="MPSAPPNVLAFAASDPTGGAGLQADILTIASLGCHPLSVLTALS
                     VQDTSGVRRLQAVDAALLAEQAQCLLAELPVAAFKVGVVASAANARAIGAILAAHPRV
                     PVVLDPVLASGRGDALAGAGVPEALCEQLMPRTTVLTPNTIEAAALGGVAACLARGCR
                     YVLLTGTHADTPDVVNTLHDAGGVVREDRWPRLAASYHGSGCTLAAALAARLALGDAV
                     PQAARAAQDATWRALERGFKPGRGQHLPWR"
     gene            10923..11528
                     /gene="thiE"
                     /locus_tag="VFB53_01840"
     CDS             10923..11528
                     /gene="thiE"
                     /locus_tag="VFB53_01840"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZN85502.1"
                     /translation="MALTLRGLYAITPERLDRVAEAIGSGALCALQYRSKSADAARRR
                     DEALALARLCRRHGVPLIVNDDLELALQVGADGAHLGREDGGLAAARARLGARILGVS
                     CYDSLAAARAAVAAGADYVAFGSVFASPTKPGAVRAPLELFSHALGVPKVAIGGITAA
                     NAPQLAAAGADAVAVISDLFDAPDVAARAREYAKVFAHGIA"
     gene            11515..12792
                     /gene="hemL"
                     /locus_tag="VFB53_01845"
     CDS             11515..12792
                     /gene="hemL"
                     /locus_tag="VFB53_01845"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZN85503.1"
                     /translation="MASRNDELHARAQARIPAGVNSPVRAFRAVGGTPPFLERASGPH
                     VWDADGKRYIDYLGSWGPMLVGHTHPAVVEAVQSAASRALSFGAPTAAEVEMAELLCR
                     LVPSMDLVRLVSSGTEATMTALRLARGHTGRSLIVKFEGCYHGHADALLVKAGSGALT
                     FGNPSSAGVPAETAAHTLVLDYNDTAGLRSLFDRRGSEIAGVIVEPVAGNMNLVLPAP
                     GFLAALREQCTRHGAVLILDEVMTGFRVALGGAQARFGIRPDLTTLGKVIGGGLPVAA
                     VGGRREIMEKIAPLGPVYQAGTLSGNPVAVAAGLATLRLVDSPGFQERVEATTRTLVE
                     GLMAQAQRAGVTFCAQSIGSMFGVYFRAAPPQSFAEVMQCDRERYNRFFHAMLARGVY
                     LAPSAYEAGFVSGAHGAREIEETLAAARAAFAA"
     gene            complement(12789..13952)
                     /locus_tag="VFB53_01850"
     CDS             complement(12789..13952)
                     /locus_tag="VFB53_01850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN85504.1"
                     /translation="MAQKQRFGWSMAASVLAVMVLVFAALVSSWLGLDAEARGRATLE
                     QAAQLERDVADARVLAATVAALRAELALDALRGEAMDPRNRRVAAYQDALEALRAALA
                     RLGGATLLERERVLLAAAQQAIERFVRIATEGAGGPRSGPRPAQLRAASAQRDAARLL
                     ADQAIVQIRALEQQVAGRAAEAAEDADESARRARVLLIVFGGSSLLLALVLARTLSES
                     IARRAELLGQLAELARVDSLTGVANRRAWDDELARGLERARRTGKPCTVALIDLDHFK
                     RFNDTHGHQRGDALLRTAAQAFAARLRNDDLIARYGGEEFAVLLHGCGLDSALQLFER
                     LQGMLPAGQTFSAGVADSDGKEESLAVVARADAALYRAKGQGRNRTLGAPRPA"
     gene            complement(13992..>14364)
                     /locus_tag="VFB53_01855"
     CDS             complement(13992..>14364)
                     /locus_tag="VFB53_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZN85505.1"
                     /translation="RVVAGASTISQQLAKNLFLSDERSLWRKGEETILTWMLEAVMTK
                     RRILELYLNYAEWGEGVFGAEAAARHHFGASAATLRDEQAVWLAAILPSPRRYEGGRT
                     TDYLARRMDVIRARMGSAQIP"
BASE COUNT         1876 a         5327 c         5065 g         2096 t
ORIGIN      
        1 cacgcggatc ttgcgcgcga acgaactggc gggggaggcg taaatcttca tgtcagagag
       61 ttgcagccct acgccacccc cgcctcctcg gcctgcttgt ccgcgtggta cgagctccgc
      121 accatcgccc cgatcgccgc gtgcctgaac tccatcgccc cggcctcgcc ctcgaacatg
      181 gcgaaggtct ccggatgcac gtagcgctcc accggtagat ggtgcggcga aggctgcagg
      241 tactgcccga tggtgagcat gtccacctgg tgcgcgcgca ggtcgcgcat cacctggagt
      301 atctcctcgt ccgtctcccc cagccctacc atcagtcctg acttggtggg gaccgaaggc
      361 accgacttct tgaactcctg aaggagtctc agggagtgca ggtagtcccc gcccggccgc
      421 gcccgccggt agagtcgcgg cacggtctcc aggttgtggt tcatcacgtc cggcggcgcc
      481 gccaggagaa tccccagcgc ccgatccagc cgcccgcgga aatccggcac cagcacttcg
      541 atggtcgttg ccggcgactt ttcccgcacc gcgcggatgc aatccacgaa atgctgcgcg
      601 ccgccatcct tcagatcatc ccggtccacg ctggtgatga cgacgtactt cagtttcagc
      661 ttcgcgatgg tgccggcaag atgccccggc tcctccgcat ccaccggcag cggccgccca
      721 tgcccgacgt cgcagaaggg gcagcgccgc gtgcagatgt cgccgaggat catgaaggtg
      781 gcggtgccct tgccgaagca ctcgccgatg ttcgggcagg acgcctcctc gcacaccgtg
      841 tggagcttct gctcgcgcag gatctgcttg atctcgtaaa accgggtgga aggactcgcg
      901 gccttcaccc ggatccacga gggcttcgcc agcggcgagc gcggcacgat cttgatcggg
      961 atgcgggcgg tcttctcccg gcccttctcc ttcacccccg cttcacgcat gttccagccg
     1021 atgcgcgatc aggcgcgcga ggcgaatgcc cacttccttt gccgtcccca tcacgccgag
     1081 atcgcgcgcc tgcaccacgc tcaggcccgg atagccgcaa gggtcgatgg cagcgaacgg
     1141 cgccaagtcc atgtccacgt tcagcgcgag gccgtgccac gcccgcccgc gcgtgacgcg
     1201 gataccgagc gaggcgatct tcgccccgcc cacgtacacc cccggcgcgc cggccttcct
     1261 ctccgccggg atctcgtagc tgatgaggaa attgatgacg cactgctcga gcatctgcac
     1321 gtaggtcttc accttgatgc cgcgccgcgc gatgtccacc atggtgtaga tcaccgcctg
     1381 ccccggcccg tggtaggtga tctccccgcc gcgctcggcc tgcaccaccg ggatgccgtt
     1441 cgccgtcttc ggcccgaact tcgcgtccgc gccctgcccc agcgtgtaca ccggcgggtg
     1501 ctcgaccacc cacagctcgt ccggcgtctc ggtgtcgcgg tgcgcggtga actcgcgcat
     1561 gcgcgcgagc accgccacgt aatcggtcag cccgaggctc ttgatgcgcg gcgcgagcgc
     1621 gacgtccatc acagcaccat caccaccgcg gggtggtcgc acaggtcctg gtacagcgcg
     1681 tccaactgct cgcgcgactg ggcgcggatg gtgcaggtca cgctgaggta ggtcccctgg
     1741 cggctggggc gcagttccat ggtggcggcg tcgaagtccg gggcgtgctt gcgcacgatg
     1801 gagaccacgg tgtgcgcgaa gcgcgcctgt ttccgcccca tcaccttgat cggaaaatcg
     1861 gtggggtagg aaaccagcga ctcttcgttc gcaatcggca tcaggccgag accgcctcct
     1921 tgcgcatgac cttcgccttg aactcctggt agcccgcgta ggccttgcgg aacagcggcc
     1981 ccggcttgcc atcgccgacc ggcttgccgt cgagcgtcgc cacggcgagg atttccttgg
     2041 aggaggaagt gagccagatc tcgtccgcgg cgcgcacttc ggcctcgctc acgtcgcgga
     2101 actcgagcgg gatcttcgtc gcctgcgcga tctccaccac cacgtcgtag gtgatgcccg
     2161 gcaggatgag gttggatttg ggcggcgaca ggagaagccc gttcttcgcc acgaacacgt
     2221 tggaggcgga ggcttcggtc agcttgccct cgcggaacag gatcgtctcc gccgcgcccg
     2281 cgtccgccga cgcctggcgc aggaggcagt tgccgacgag cgagatcgac ttgatgtcgc
     2341 agcgcagcca gcggttgtcc acggcgctca ccgcggcggc gcccttttcc accagttcct
     2401 tcggcgggtt gaccagcggg ttgcacatga cgaacaccgt gggctccacg ccagccggga
     2461 aggcgtggtc gcgcttcgcc acgccgcgcg tcacctggaa atacacggcc tggtcctgcc
     2521 agggttgctg cgccacgagc ttcaggatga tctcgcgcca ctgttcgcgc gggtacgggt
     2581 tcctgatgcg caccgcggcg aggctgcgct ccaggcgcgc caggtgctcg tccatgcgga
     2641 acggcgcgcg cgagtacacc gggatcagct cgtagacccc gtcgccgaag atgaagccgc
     2701 ggtcgagcac cggcaccatc gcctgctcga tcgggaggaa cttgccgttc aggaaaacca
     2761 tgcggccctc gctactcgaa ccacaaacgc agtgtatccc aggcgcggcc gaagaaaccc
     2821 gccgcgggca ccgctgcgag cgccgtcacc gggtattcgc cgagcggcgc gccgcccagc
     2881 gcgacgcgca gcgtgcccac gcgctgcccc ttggcgaccg gcgccagcac cggcggcagc
     2941 gtcgccagtt ccgccttgag cagcgcggcc tcgcccttgg gcaccgtcac caccagcccc
     3001 tgcgcgaacc cggcgggcac cagggcggcg gcgcctttcc acaccgcgat cgacttcacc
     3061 gcctcgcccg gcgcatagag cttcacgccg tcgaaattct ggtagcccca gttgagcagc
     3121 ttctgcgact ctctcgcgcg cgcgccttcc gtgctcgcgc cgagcagcac cgagatcaag
     3181 cgccgcccgc cgcgcgccga ggatgcgatc aggcagtagc ccgcctcctc ggtgtggccg
     3241 gtcttcaccc cgtcgacgct cgggtcgagc cacagcagcc ggttgcggtt ctgctgcgtg
     3301 atgccgttgt agctgaattc cttctgcgcg tagtagcgcg cgtagtcggc gggaaaatcg
     3361 cggatcagcg cgccggcgag cagcgccagg tcgcccgccg tgctggcgtg ccccggcgcc
     3421 ggggcgccag tggcgttggc gaagctcgtc tgccgcatgc cgaggcgttc cgcctcgcgg
     3481 ttcatcagtg cgacgaacgc gtcttccgag ccggcgagcg actcggcgag cgcgagcgcc
     3541 gcgtcgttgc ccgactgcac caccacgccg cggatcagct cctccacgct caccgcgcgg
     3601 cccggctcga tgaacatgcg cgagccgccg gcgcgccagg cgcgctgcga cacctgcacc
     3661 tgcgccgccg gttccaggcg cttgtcgcgc agcgcgccga acaccacgta ggcgctcatc
     3721 agcttggtga gcgaggcgat ggcgaagcgc tcgccggccc tttccgcggc cagcacctgg
     3781 ccgctgctga ggtcggcgag cacccaggcc cgggcggcaa gctgcggcgc gacgggcggc
     3841 gccgccgtcg cagccgtgca ggcgaggagc agcaccccgg cgaccggacc ggcgcgcatg
     3901 cgagcgccgc cctagcgcag cgcgagcggc gcgccgcccg gcaccacctg ctcgaccagc
     3961 acgcgcgcgc tgcccgcggc aatgtagccg aggcggtgcg cggcggcgta cgacaggtcg
     4021 atgacgcggc cgtcgtggaa cgggccgcgg tcgttgatgc gcaccaccac cttgctgccg
     4081 ttggcgagat tggtgacgcg cgcatagctc gggatcggca ggatcgggtg cgcggcgctc
     4141 atcttgtaca tgtcgtaggc ctcgccgctg gaggtcggct tgccgtggaa gcgtttgccg
     4201 taccagctcg ccgcgccgcg ctgctcgaat ggcccgaccc gcgtcagggg cacgtagcgc
     4261 ttgcccagcg cctcgtaggg gcggttggca tagcggtgca gcggctcatg gcgcgggacg
     4321 gcgtccggaa tcgaggcaag ctttgcgctg tctccgggcc cgtcgtcctt gtagtagccg
     4381 ccggggcgct tgtcgggcgt cgaactgcag cccgcggcca agagcagcgc cagcgcgatg
     4441 cgcctcatgc ctcgtagacc acctggcccg cgacgacggt ggcgcgcacg cggccggcga
     4501 gctccatgcc gaggaagggg gagttcttgc cctggctctt cagggcttcc ggcgtcacgc
     4561 gccagcttgc ctgggggtcg aacagcacca ggtcgcccgg cgcgcccggc tccaggcgcc
     4621 ccgaggccgc gccgaggatg cgcgccggcg cgctgctgat gcgcgcgagc gtctgcgcga
     4681 gcgtgagttt gcgttccatc ccccacttga gggtcagcgg cagcagaagt tccagcccgg
     4741 tcgcgccggg ctcggcttcg gcgaagggca ggtgcttggc gtcctcgtcc accggcgcgt
     4801 ggtcggacac cgcgcaatcg atggtgccgt cggccaggcc cgcggcgagc gcttcgcggt
     4861 cgcgctggct cctgagcggc ggctccagcc ggcactgcgc gtcgaagtag cccaggtcca
     4921 tgtccgagag gtgtacgtgg tggatgccca cgtcgcaggt gatcggcagg ccgtcgtgct
     4981 tggcgcggcg cagcagcgcc acgccagcgg cgctcgagat gcggcacagg tgcacgcgcg
     5041 cgccggtggc gcgcaccagc tcgaagatgg cggcgagggc gatggtctcg gcgaacgccg
     5101 ggatgccggc aaggccgagg cgagtggcca cttcgccatc gtgcgcgacg ccgcccctgg
     5161 cgagccaggc gtcctcgggg cgcagccaca ccggcaggcc gaatgtggcc gcgtattgca
     5221 gcgcgcgcca caggacctgc gtgtcctcga cggcggcatt gccctgcgag aacgcggcgc
     5281 agcccgcctc ggcgagctcc gccatctcgg tgagctgccg cccggcgagc ttcaccgtga
     5341 gcgcgcccac cgggtagacg cgggcgcgcg acagggcttt cgcccgccgc cgcagcatgt
     5401 ccaccaggcc cggctcgtcg agcggcggat cggtgtccgg cgggcaggcg aggctcgtca
     5461 cgccgcccgc caccgcggcg tccatctccg attcgagcgt cgccttgtgc tcgtagcccg
     5521 gctcgcgcag gcgcgcggcg agatccacca gccccggggc gaccaccagt cccttcgcat
     5581 ccagcgtgcg gtccggcgtg cccggcgcgg cggcgatctt gccgtcggcg atgaacacgt
     5641 cgcccaccgc gtcgcgcccg cttgcgggat ccaccaccct gccgcccttg acgaggagct
     5701 tcatccgccc cctgcgacca tgctcatcac cgccatgcgc accgcgatgc cgaaggtgac
     5761 ttgcggcagg atcaccgagc gcggcccgtc ggccaccgcc gagtcgattt ccacgccgcg
     5821 gttcatcggc cccgggtgca tgacgatggc gtcgggcttg gccagcgcga gcttctcggc
     5881 ggtgagcccg tagtgcttgt tgaattcctg cgcgctgggc aagagcgcgc ccgtcatgcg
     5941 ctcgttctgc agccgcagca tcaccaccac gtcggcgccg cgcaggcctt cgcgcatgga
     6001 gtggaacacg cgcacgccga gcgccgcggc gccggcgggg atcagggtct gcggcccgat
     6061 gacgcgcact tccggcgcgc cgagcgtggt gaggccgtgg atcagggacc gcgcgacgcg
     6121 cgaatgcagc acgtcgccca cgatcgccac cgtgaggccg ctgaaatcct tcttgtagtg
     6181 ccggatggtg tagaggtcca ggagtccctg cgtcgggtgc gcgtggcgcc cgtcgccggc
     6241 gttgatcacg tgctcgtgcg gcttcacgtg gcgcgcgacc aggtgcgccg cccccgatga
     6301 gccgtggcgc accacgaaga tgtcggcctg catcgccgag aggttggcca ccgtgtcgag
     6361 cagcgattcg cccttggcgg tcgagctcac cgcgacgttg aggttgatga cgtcggccga
     6421 caggcgcttg gcggcgatct cgaaggtggt gcgcgtgcgc gtcgagcctt cgaagaacag
     6481 gttgaacacg cttttccccc gcagcagcgg caccttcttg atgtcgcgct cggtgacgcc
     6541 ggcgaaggat tccgccgtgt cgaggatctt caggatcagc tcgcgcggca ggccctcgat
     6601 cgagagcaga tgctgcagct cgccgttggc gtcgagctgg ggattacccg gcacgctcga
     6661 gctccaggga aagccggccc gcgccgtcgc gcctcagtcg caggcgcatg ccggcggcga
     6721 cctcgacctt ggcgccggcg agctgcgcgc acaccggcag ctgccggccg tcgcgctcgg
     6781 cgagcaccac caggacgatc gacgccgggc ggccgtagtc gaacagctcg ttcatcgccg
     6841 cgcgcaccgt gcgcccggtg tagagcacgt cgtccaccag caggaggtcg cgcccttcga
     6901 ccgcgaacgg gatcttcgag cgcttgggat ccctcgccag gccctgcttg gcgtagtcgt
     6961 cgcggtagaa ggcggggtcg agcaggccga gcggctcctt gatcccgagc gccgcgtgca
     7021 ggcgctcggc gacccaggcg ccgccggtgt ggatgccgac catggcgctc gccggcgtca
     7081 cgcgcgcctt cagctcgcgc gcgagctgcg tgcacagctt ctccgcgtca ggcagcttgc
     7141 tcatggaaat actgctcgag gatcagctgc gcggcgaccg agtccacggg ctttcttttg
     7201 cggccgcgca ggaggctctc ggccgccacc gaggtgtagc gctcgtcgac ctgcgccacc
     7261 ggcaggcgga agcgcccctc gagctggcgc gcgaagcgct gcacgcgccg ggcgagcggg
     7321 tgctcgccgc cctcggccac cggcaggccg accaccagcg ccgcggggcg ccattccttc
     7381 accagtttgc cgatggccgc gaagccgggc gcggcgatgc cgggcagcgg atgcgcggtg
     7441 ccgagctccg cttcccctac cgcaacgcca atgcgcttga tgccgaaatc gaaggcgagg
     7501 acggtgccgg cctcaggcat gtcccgccga atccgccagg cgcgcgtagt ccacgccgag
     7561 cagttccatc gccgccggca ggcgctcctc cgagggcagg tcgaagatga tcgcgtcctt
     7621 cgcctcgacc gtgagccagg cgttctgccc cagctcgtgc tcgagctgcc ccgccgacca
     7681 gccggcgtag cccagcgtca ccagaatctt ctgcgggccc tcgccgcggc ccaccgcttc
     7741 gaggatgtcc ttggaggtgg tgaggccgat cgcgtcgcgc accttcagcg tggactgcca
     7801 gctgcccgcc ggctcgtgca gcacgaagcc gcggtcggtc tgcaccggac cgccgaagta
     7861 gatcggcgcg tcggacaggt ccttgtgctt cagggtgagc gacaggcgct cgaacagcgc
     7921 ctgcagggtc atgtcgatgg ggcggttgat cacgacgccg agcgcgccct gggcgttgtg
     7981 ctcgcagaca taggtcaggg acttcgcaaa gtacggatct gccatgctgg gcatggcgat
     8041 caggaagtgg tgcgtcaggt tgatcgagga cacgcgcagg gggcatttta ccgcatgccc
     8101 cccgggcgtg cctctcaata gcgcagcttc tccagcgcct cctgcagctc gcgcagcagc
     8161 cccgccacct cctccaccga ggccgcggcg ccggtcgcgg cgctcgccgc ctgctcggcc
     8221 ccggagagca tttcccgcac cccgccggcg atggcgctcg ccgccgcgcc ctgcgattgc
     8281 gcggtcttcg acacctgttc gatccggtcg gccagctcgc gcagcagctc gcccagctcg
     8341 gccagggtgc gccccgaggc gctgccgccc agcgcttcgg cctgcgccgc gatctggctg
     8401 agcagctgcg cggtcttcgc catctcgggc gaggcgccgc gcacccggtc cacgctctgc
     8461 gcggcgaggc gcacgcggcg gccgatctcc tccaccacgg agagcgccgc ctgcaccgcc
     8521 tggcggccct gggtggcggc ggcgagcgcg gcgcgcgcca tctccgccga ctggttggag
     8581 cgcgccgaca ggtcggtcac ggcgcgcgcc atgccgagcg cgcgcgcgct cgcgcccttg
     8641 atgcgctcgg attgcaggcg cgccgaatcc agcagcccgt cggcggcggc ctgcgccgag
     8701 ccggccgcgc cgccgacgcg ttcggcggcc actgcgaccc gcgccaccag cgcgcgcagc
     8761 gcgtccagcg cgtcgttcag cgccgcggcc gcgggctggg tgatctcgct gcccacgctg
     8821 gcgcgcgcgg tgaggtcgcc cgtggcgagg ccttcgagtt cgcggcgcag cttctccacc
     8881 gcctccaggt cgcgctcgtg gtagcggcgc agcaccccga ggcgccgcgt cgcgctgtcg
     8941 cgcgcgcgcc gctcgtcgtg cgcgaaggcg agcgcggcga gggcgagcgc gccggcgagc
     9001 gccagcacga tcgccgccag cccagcctcg taggcgcggc tggcgggcgc gtcacgcgtg
     9061 gccgctacca ggccgtcgac cgactgcagc aggcgctcgg catcgtcgta tacggccagc
     9121 gccgcggcct tggcgccgcc gaggcgcgcg cgcgttccga gcaccgccac caccgcgtcc
     9181 tggtagccgg caaaggtttc gcccagggcg gcgagcgccg ccgccgtctc ctcgtcgcgc
     9241 gcgccggcgc gcaggcgttc cagcgtgccg cggaattcgt tcacgtcctt gccgaggagg
     9301 aactcgacct cgggctcgac gccgtcggcg gcgagcagct gctgcgcgcc gcgcgccatg
     9361 cgctgggtga gcatcaccag gcggctcgcc gcggcgactt ccgccgcggc cgcgccgcgc
     9421 gcgagcttca ggttctgcac gcgttcggcg cgctccagga ggcgcggatt gccgcgctcc
     9481 atcccggcgg cggcggtggc gagtgcggtg agcagcttct ggtcgcgcag cagcgtggcg
     9541 gcgttcctct cggtgcgctc ccagtcgcgc tcgagcgcgc gcagcgcctg cggcgcgcgg
     9601 tcctcgcgcg ccaggagcgc caggccggcg gcgaagccgt cgcggctcgc ctggatctgc
     9661 gcgaaggcct cgggctcgcc gcgcaggctg cgcagcgagg ccttcgccag ccgctgcgac
     9721 agcatgcgca attcgcccgc cgccgcgacg tggccggcgc cgaaatgcgc ttcgcggtat
     9781 tcggcccatg cgagccacag cgcggcggcg agcaaggcgg cggtcgccgc ggcgagcagg
     9841 gccagctggc ggcgcagcgg cagctccgcc acgccgcgcc aggccgcgcc gagcgtcttc
     9901 aggtccaagc gcggcggcgc gcgctcagat ctcgaccatc tcgaaatctt ccttgcgcgc
     9961 gccgcattcc gggcaggtcc agttgatggg cacgtcttcc cagcgcgtgc ccggcgcgag
    10021 cccctcgtcg ggcatcccgg cggcctcgtc gtagacgaag ccgcagatca ggcacatcca
    10081 ggtcttgaac tgcacttggt tctcggtcat caggtgcgag ggggcgctgg gttaaaatcg
    10141 tgcggcatct tagcgtattc cgcggcgcgg ccgcgccgcg cccccatgcc ttccgccccg
    10201 ccgaacgtcc tcgctttcgc cgcctccgat cccaccgggg gcgcgggcct gcaggccgac
    10261 atcctgacca ttgcgagcct gggctgccat ccgctctcgg tgctcaccgc gctcagcgtg
    10321 caggacacga gcggcgtgcg ccgcctgcag gcggtggacg cggcgctgct cgccgagcag
    10381 gcgcaatgcc tgctcgccga gctgccggtg gcggcattca aggtcggcgt cgtcgccagc
    10441 gcggcgaacg cgcgcgcgat cggcgcgatc ctcgcggcgc atccccgcgt tccggtggtg
    10501 ctcgacccgg tgctcgcctc gggtcgcggc gatgcgctgg ccggggcggg cgtgccggag
    10561 gccctgtgcg agcagctgat gccgcgcacc acggtgctca cgccgaacac tatcgaagcg
    10621 gcggcgctcg gcggcgtcgc ggcctgcctg gcgcgcggct gccgctatgt gctgctcacc
    10681 ggcacgcacg ccgatacgcc cgacgtggtg aacacgctgc acgatgcagg tggcgtggtg
    10741 cgcgaggacc gctggccgcg cctcgccgcg agctaccacg gctcgggctg cacgctcgcc
    10801 gcggcgctcg cggcgcgcct ggcgctcggc gacgcggtgc cgcaggcggc gcgcgcggcg
    10861 caggacgcca cctggcgcgc gctggagcgc ggcttcaagc ccggccgcgg gcagcacctg
    10921 ccatggcgtt gacgctgcgc gggctgtacg ccatcacgcc cgagcggctc gatcgcgtcg
    10981 ccgaggcgat cggctcgggc gcgctgtgcg cgctgcagta ccgcagcaag tccgccgacg
    11041 ccgcgcgccg gcgcgacgag gccctggcgc tggcgcggct ttgccgccgc cacggcgtgc
    11101 ccctgatcgt caacgacgac ctggagctcg cgctgcaggt gggcgccgac ggcgcgcacc
    11161 tcgggcggga ggacggcggg ctggccgcgg cgcgcgcgcg cctgggcgct cgcatcctcg
    11221 gcgtgtcctg ctacgactcg ctcgcggcgg cgcgcgccgc cgtcgccgcc ggcgccgatt
    11281 acgtcgcctt cggctcggtg ttcgcctcgc ccaccaagcc cggcgcggtg cgcgccccgc
    11341 tcgagctgtt ctcccacgcg ctcggcgtgc cgaaggtcgc aatcggcggc atcaccgcgg
    11401 caaacgcccc gcagctcgcc gccgcggggg cggatgcggt ggccgtgatc tcggacctgt
    11461 tcgatgcacc ggatgtggct gcgcgcgcgc gggaatacgc taaggtgttc gcccatggca
    11521 tcgcgtaacg acgagctcca cgcgcgcgcg caggcgcgca tccccgccgg cgtgaattcg
    11581 ccggtgcgcg ccttccgcgc cgtcggcggc acgccgccgt tcctcgagcg cgcctcgggc
    11641 ccgcacgtgt gggatgccga cggcaagcgc tacatcgact acctcggctc ctggggaccg
    11701 atgctggtcg gccacacgca cccggcggtg gtcgaggcgg tgcagtcggc cgcctcgcgc
    11761 gccctgtcct tcggcgcgcc caccgcggcc gaggtggaaa tggcggaact cctgtgccgc
    11821 ctggtgccct cgatggacct cgtgcgcctg gtctcctcgg gcaccgaggc gacgatgacg
    11881 gcgttgcgcc tggcgcgcgg ccacaccggg cggtcgctga tcgtcaagtt cgagggctgc
    11941 taccacggcc atgccgatgc gctgctggtg aaggcgggct cgggcgcgct caccttcggc
    12001 aacccgagct cggccggcgt gcccgccgag accgccgcgc acaccctggt gctggattac
    12061 aacgacacgg cgggcctgcg ctcgctgttc gatcggcgcg gcagcgagat cgccggcgtg
    12121 atcgtcgagc cggtggccgg caacatgaac ctcgtcctgc cggcgccggg attcctcgcg
    12181 gccttgcgcg agcaatgcac ccgccacggg gcggtcctga tcctcgacga agtgatgacc
    12241 ggttttcgcg tcgcgctcgg cggcgcgcaa gcgcgcttcg gcatccgccc cgacctcacc
    12301 acgctcggca aggtcatcgg cggcggcctg cccgtggcgg cggtcggggg ccggcgcgag
    12361 atcatggaga aaatcgcccc gctcggcccg gtgtaccagg cgggcacgct ctcgggcaac
    12421 ccggtggcgg tggccgcggg cctcgcgacg ctcaggctgg tggattcccc cggtttccag
    12481 gagcgggtcg aggccaccac gcggacgctg gtcgagggcc tgatggcgca agcccagcgc
    12541 gccggcgtga ccttctgcgc ccagtccatc ggcagcatgt tcggcgtgta cttccgcgct
    12601 gcgccaccgc aatccttcgc cgaagtgatg cagtgcgacc gcgagcgcta caaccgcttc
    12661 ttccacgcca tgctcgcgcg cggcgtgtac ctcgccccct cggcgtacga ggcgggcttc
    12721 gtctccggcg cgcatggcgc gcgcgagatc gaggagacgc tcgccgcggc gcgcgccgcc
    12781 ttcgccgcct aggcgggccg cggcgcgccg agcgtgcggt tgcgcccctg ccccttggcg
    12841 cgatacagcg ccgcatcggc gcgcgccacc accgccaggc tttcctcctt gccgtcgctg
    12901 tcggcgacgc cggcggagaa ggtctgcccc gccggcagca tgccctgcag ccgctcgaac
    12961 agctgcagcg cgctgtccag gccgcagccg tgcagcagca cggcgaattc ctcgccgccg
    13021 tagcgcgcga tcaggtcgtc gttgcgcagg cgcgccgcga acgcctgcgc cgcggtgcgc
    13081 agcagcgcgt cgccgcgctg gtggccgtgc gtgtcgttga agcgcttgaa gtggtccagg
    13141 tcgatgagcg ccacggtaca gggcttgccg gtgcgccgcg cgcgctccag gccgcgcgcc
    13201 agctcgtcgt cccaggcgcg gcggttggcc acgccggtga ggctgtcgac gcgcgccagc
    13261 tccgccagct gcccgagcag ctcggcgcgc cgcgcgatcg actccgagag cgtgcgcgcg
    13321 aggaccagcg ccagcaggag cgagctgccg ccgaacacga tgagcagcac gcgcgcgcgc
    13381 cgcgccgact cgtcggcgtc ctccgcggct tcggcggcgc gccccgccac ctgctgctcg
    13441 agggcgcgga tctgcacgat ggcctggtcg gcgagcagcc gggccgcatc gcgctgcgcg
    13501 ctggccgccc gcagctgggc ggggcgcggg ccgctgcgcg ggccgccggc gccctcggtg
    13561 gcgatgcgca cgaagcgctc gatcgcctgc tgcgcggcgg cgagcagcac gcgctcgcgt
    13621 tccagcagcg tcgcgccgcc gagccgcgcg agcgcggcgc gcagcgcttc gagcgcatcc
    13681 tggtaggcgg caacgcgccg gttgcgcgga tccatggctt cgccgcgcag cgcatcgagt
    13741 gccagttcgg ctctcagcgc cgccaccgtg gcggcgagca cgcgcgcgtc ggccacgtcg
    13801 cgctccagtt gcgccgcctg ctcgagcgtg gcgcgcccgc gtgcctcggc atccagcccg
    13861 agccaggagg acaccagcgc cgcgaacacc agcaccatca cggcgagcac cgatgccgcc
    13921 atgctccagc cgaagcgctg cttttgcgcc atgcccccgt tccgccttat ggacgcgcgg
    13981 acaaaagtct atcaggggat ctgcgccgac cccatgcgcg cgcggatcac gtccatgcgg
    14041 cgcgcgagat agtccgtggt gcgcccgccc tcgtagcgcc gcgggctggg caggatcgca
    14101 gccagccaca ccgcctgctc gtcgcggagg gtcgcggcgc tcgcgccgaa atggtggcgc
    14161 gcggcagctt cggcgccgaa cacgccttcg ccccattcgg cgtaattcag gtagagctcc
    14221 aggatgcggc gcttggtcat caccgcctcg agcatccagg tgaggatcgt ctcctcgccc
    14281 ttgcgccaca gcgagcgctc gtcggagagg aacaggttct tcgccagctg ctggctgatg
    14341 gtcgaggccc ccgccaccac cctg
//
LOCUS       DASRIE010000043        10891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_47159, whole genome shotgun sequence.
ACCESSION   DASRIE010000043 DASRIE010000000
VERSION     DASRIE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47159"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFB53_01860"
     CDS             complement(<1..388)
                     /locus_tag="VFB53_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZN85506.1"
                     /translation="MRSSLAALALAAGAALAQDAALEKRVMGLAHELRCLVCQNQTIA
                     DSSAPLAVDLRNQIREQLARGASEREVREFMVARYGDFVLYKPPLKATTLALWFGPFL
                     LLAAGALLLWRRLARRRAPAPPLSEAE"
     gene            complement(378..905)
                     /locus_tag="VFB53_01865"
     CDS             complement(378..905)
                     /locus_tag="VFB53_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HZN85507.1"
                     /translation="MRLAWVLAAFAALVGLLAAGLTRNPREVPSPLIGKPAPAFQLPQ
                     LHAPEKRFAQKDMLGKAWVLNVWASWCPPCLVEHPVVTALAKSGLAPVVGLNYKDRRE
                     DALPWLKRNGDPYQLSVADADGRIAMDYGVYGVPETYVIDRRGTIRYKHIGPLTPEVV
                     RDKVEPLLRELARAL"
     gene            complement(902..2833)
                     /locus_tag="VFB53_01870"
     CDS             complement(902..2833)
                     /locus_tag="VFB53_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZN85508.1"
                     /translation="MIPELGQLALSLALVVALVQGILPLAGAARGDAAWMGVARPAAH
                     AQALLVAFAFGCLVHSFVTSDFSVANVAMNSNSALPLHYRFAAAWGSHEGSLLLWCLM
                     LGVWMSAVGLFSRHLPEEMAARVLGVLGLASAGFLAFLLFTSNPFERLFPAPADGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAYAFAVAALLSGRLDAAWARWSRPWTTVAWCFL
                     TLGIALGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGAFRSWTV
                     LLAIVAFGLSLLGTFLVRSGVLTSVHAFAVDPKRGIFILGLFAATIGGALALYAWRTA
                     RIGLGGSFEPVSRESFLLANNVLLGAAAGSVLLGTLYPLLLDAFGGGKISVGPQYFEA
                     VFAPLMAPALFLMGVGPLARWKRASLPELAVLLRWALAASLAVGLLLPLAFGQWKALV
                     GLSLALALWIAATGLVTLRKGLRQTRSHYGMVLAHLGVAVFVAGVTLVKGYESEQDAN
                     LKPGDSVALGPYEFRLESVRPVQGPNYAAAQARISVTRGGRPVAVLAPERRTYAVQDQ
                     TMTEAAIDTGLARDLYVSLGDPLPGGAWLVKVQVKPFIDWIWGGCLLMALGGLLAASD
                     RRYRVSVPAAQRETAEAAA"
     gene            complement(2830..3264)
                     /gene="ccmE"
                     /locus_tag="VFB53_01875"
     CDS             complement(2830..3264)
                     /gene="ccmE"
                     /locus_tag="VFB53_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZN85509.1"
                     /translation="MKPRHKRLAAIIGGLAALGAATALVLSAFEQNLVFFFTPTQVAA
                     KEAPLGRTFRIGGMVEKGSVKRDGVEVRFLVTDTAKTIPVVYRGALPDLFREGKGVVA
                     QGQLGADGVFRAREVLAKHDENYMPPEAAHAVDKAQKTIRRP"
     gene            complement(3248..3367)
                     /gene="ccmD"
                     /locus_tag="VFB53_01880"
     CDS             complement(3248..3367)
                     /gene="ccmD"
                     /locus_tag="VFB53_01880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZN85510.1"
                     /translation="MSEFLAMGGYAWYVWGSYGVTFLCLLIEIVVLLKREAKT"
     gene            complement(3364..4059)
                     /gene="ccmC"
                     /locus_tag="VFB53_01885"
     CDS             complement(3364..4059)
                     /gene="ccmC"
                     /locus_tag="VFB53_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HZN85511.1"
                     /translation="MRWFWYASPQTFFPLAGTIARWCGIAAALLAAVGLYIGFFVAPT
                     DAQQGEAYRIIFIHVPAAWMSMLIYVVMASWAALGLALNTRLSGMMASALAPTGALFT
                     FIALWTGALWGRPTWGTYWVWGDARLMSELILLFLYFGFMALRASIDDPRRADRAAAL
                     LAIVGVVNIPIIYYSVQWWNTLHQGASVSLTAAPKMAATMLTGMLVMALAFWMYSIAA
                     AMLRVRSIIRERA"
     gene            complement(4056..4724)
                     /gene="ccmB"
                     /locus_tag="VFB53_01890"
     CDS             complement(4056..4724)
                     /gene="ccmB"
                     /locus_tag="VFB53_01890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZN85512.1"
                     /translation="MIAAARCILRRDLLLALRRRGDVATALLFFVIVASLFPLGVGAE
                     PNLLRAVAPGVIWVSALLSSMLSLPRLFADDHADGTLEQMLLGAAPLGVIVAAKLLAH
                     WLVAGLPLVAIAPLLALQYDLPPPLFGTLALSLLLGTPVLSLIGAIGAALTLGLRGGG
                     VLLALLVLPLYVPVLIMGAGSVEMAGAGLGGQAQLMLLGAMLIVAAAFAPWAIAAALR
                     ISSE"
     gene            complement(4721..5332)
                     /gene="ccmA"
                     /locus_tag="VFB53_01895"
     CDS             complement(4721..5332)
                     /gene="ccmA"
                     /locus_tag="VFB53_01895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HZN85513.1"
                     /translation="MLEAVGLRSARQGRQLFKDLDFALRGGELLRVAGENGSGKTTLL
                     KILAGLLAPDAGEVRWRGTPIRGLREEYSRELVYLGHAPAVKGDLTAAENLGIACTIA
                     GADAQAQHVEGALASYGVPGDVPLRRLSQGQRRRAALARLLLAAAAPLWLLDEPFAAL
                     DAAATRLTEELIAGHVARGNAVVYTTHQEAGIGAAVQRVVALG"
     gene            complement(5346..5636)
                     /locus_tag="VFB53_01900"
     CDS             complement(5346..5636)
                     /locus_tag="VFB53_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85514.1"
                     /translation="MARIAEERLEAVRKYLHQVFPDWALAERWDSDHEAHSFLLKKPR
                     EPVHLLKVSRALLDDCGPRKLAGLLEAKQVASALREAQTHRLLLTERGLDPI"
     gene            complement(5698..6069)
                     /locus_tag="VFB53_01905"
     CDS             complement(5698..6069)
                     /locus_tag="VFB53_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HZN85515.1"
                     /translation="MSAEKNVLGGELMACSFSPLTGYFRDGSCATHGADGVAHLVCVK
                     VTQEFLDFSVAKGNDLVTPRPEMRFRGLQAGDRWCLHVLRWAEAWRAGAAPQVVLEAT
                     HESVLEVVPLSALKQRALDLN"
     gene            complement(6066..7430)
                     /gene="thrC"
                     /locus_tag="VFB53_01910"
     CDS             complement(6066..7430)
                     /gene="thrC"
                     /locus_tag="VFB53_01910"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZN85516.1"
                     /translation="MRYLSTRGGAEPQRFSDILLEGLAGDGGLYVPEAYPRADLAAWR
                     GLRYPDLAAAVLRLFMDDVPGLDALARRTYTREAFGSGDIAPLKTLEPGLHLLCLSNG
                     PTLAFKDMALQLLGNLFEQVLGARGASLNVLGATSGDTGSAAEHALRGKRGVQVFMLS
                     PHGRMSAFQAAQMYALQDANIHNLAVQGSFDDCQALVKQVNADAAFKARHRIGAVNSI
                     NWARVAAQVVYYFWGYFRATRSDAEQVDVAVPSGNFGNIHAGYVARAMGLPVGRLVLA
                     TNENDVLDEFFRSGRYRVRRAAQATSSPSMDISRASNFERYVFDLVGRDGARVRALWA
                     ELDKSGEFRLPPGPGFASGQSTHADRLATIRMAHAKFGVVIDPHTADGVKVGLAHRRP
                     GVPLVCLETALPAKFSSTIVEALGREPERPARFAGLEALPQRCELIDADAVALKRYLE
                     EHAA"
     gene            complement(7435..7620)
                     /locus_tag="VFB53_01915"
     CDS             complement(7435..7620)
                     /locus_tag="VFB53_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85517.1"
                     /translation="MRPWAALALACCLAPAWAQSPAPAQKQARKPVPVKAQKAHRKAT
                     PEQIRRFNQLQQKSEKR"
     gene            complement(7617..8927)
                     /locus_tag="VFB53_01920"
     CDS             complement(7617..8927)
                     /locus_tag="VFB53_01920"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZN85518.1"
                     /translation="MKPIAVGLLGIGTVGSGTWQVLSRNAGEIRRRAGRAIRIARVAD
                     KDQKKARKLVGGRARVSGNAWEVVRAPDIDIVVELIGGTGIAKDLVLEAIAHGKHVVT
                     ANKALLAKHGNRIFAAAQKKGVMVAFEASVGGGIPIIKALREGLAANRIEWIAGIING
                     TSNFILSEMRDRGSPFATVLAEAQAKGYAEADPTFDIEGIDAAHKLTILSALAFGIPM
                     QFPKAYTEGISGLTQADIGYAEELGYRIKLLGITRRARRGIELRVHPTLVPARRLIAN
                     VEGVMNAILVKGDAVGATLYYGAGAGSLPTASAVVADLVDVTRLITADPEHRVPHLAF
                     QHDQMSDEPILPIGEVETSYYLRMRVLDRPGVLARITRILADSRISIDAMVQKEPGEG
                     EQRVDIVMLTHRAIEKNVDAAMARIERLPTVLGKVTRIRLEELL"
     gene            complement(8924..10120)
                     /gene="alaC"
                     /locus_tag="VFB53_01925"
     CDS             complement(8924..10120)
                     /gene="alaC"
                     /locus_tag="VFB53_01925"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HZN85519.1"
                     /translation="MREFSRIKRLPPYVFNITNELKMAARRRGEDIIDLSMGNPDGPT
                     PQHIVDKLVEASRRPDTHGYSVSRGIPRLRRAICDWYQRRYAVTLDMDKEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTPGVDFFAELERAIG
                     ELIPKPKMLILGFPSNPTAMCVELDFFERVIALAKQHDILVVHDLAYADITFDGWKAP
                     SILQVPGARDVAVEFFTMSKSYNMAGWRIGFMVGNAELVHALARIKSYHDYGSFTPVQ
                     VASIVALEGPQDCVGEIRQNYQRRRDVMVKGLHDIGWMVEAPKASMYIWAKIPDFYAK
                     MGSIEFTKRLMQEAKIAVSPGIGFGEYGDGHVRIALIENEHRLRQALRGIREMFKKDG
                     LLRGAA"
     gene            10136..10504
                     /locus_tag="VFB53_01930"
     CDS             10136..10504
                     /locus_tag="VFB53_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HZN85520.1"
                     /translation="MKLHASALTRLHRFTGYGAGYVMVNGTRHESSLLVHPERIEPWP
                     VAGFAALAEAHFARIAELGPEVVLLGTGDRLRFPHPGLVAPLARARIGFEAMDLQAAC
                     RTYNILMAEERNVIAALLFA"
     gene            10501..10839
                     /locus_tag="VFB53_01935"
     CDS             10501..10839
                     /locus_tag="VFB53_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZN85521.1"
                     /translation="MSLVSFAYVALAVVLGTGAQLLLKAGTNATPVGLALALDPRVLG
                     GVACYGVSLVAWILALSRTPVSVAYPMVSIGFALNALLAWWLLGEALNAQRIAGIAVI
                     MLGVWLVARS"
BASE COUNT         1681 a         3919 c         3779 g         1512 t
ORIGIN      
        1 gctcggcctc gctgagcggc ggcgccggcg cgcgccggcg cgccaggcgc cgccacagca
       61 gcagcgcgcc cgcggcgagg agcaggaaag ggccgaacca gagcgccagg gtcgtcgcct
      121 tgagcggcgg cttgtacagc acgaagtcgc cgtagcgcgc caccatgaac tcgcgcacct
      181 cgcgctcgct cgcgccccgg gcgagctgct cgcggatctg gttgcgcagg tccaccgcca
      241 gcggcgcgct ggagtcggcg atggtctggt tctggcacac caggcagcgc agctcgtggg
      301 cgaggcccat gacgcgcttc tccagcgccg catcctgcgc cagcgccgct cccgctgcga
      361 gcgccagcgc ggcgaggcta gagcgcacgc gcgagctccc gcagcagcgg ctcgaccttg
      421 tcgcgcacca cctcgggcgt gagcggcccg atgtgcttgt agcggatcgt gccgcgccgg
      481 tcgatgacgt aggtttccgg cacgccgtac acgccgtagt ccatggcgat gcgcccgtcg
      541 gcgtcggcca cggagagctg gtagggatcg ccgttgcgct tcagccaggg cagcgcatcc
      601 tcgcgccggt ccttgtagtt cagccccacc accggcgcga ggcccgattt cgccagcgcc
      661 gtgaccaccg gatgctccac caggcacggc gggcaccagg aggcccacac gttgagcacc
      721 caggccttgc ccagcatgtc cttctgcgcg aaccgcttct cgggcgcgtg cagctgcggc
      781 agctggaacg ccggcgccgg cttgccgatg agcggcgacg gcacttcgcg cgggttgcgc
      841 gtcaggcccg cggcgagcag ccccaccagg gcggcgaagg ccgccagcac ccacgccaat
      901 ctcacgccgc ggcctccgcc gtctcgcgtt gcgcggcggg caccgacacg cggtacctgc
      961 ggtcggacgc ggccagcagc ccgcccagcg ccatcagcag gcacccgccc cagatccagt
     1021 cgatgaacgg cttcacctgc accttcacca gccaggcgcc ccccgggagc gggtcgccga
     1081 gcgacacgta caggtcgcgc gcgaggccgg tgtcgattgc cgcctcggtc atggtctggt
     1141 cctgcaccgc gtaggtgcgc cgctccgggg cgagcaccgc caccggcctg ccgccgcgcg
     1201 tgacgctgat ccgggcctgc gccgcggcgt aattcggccc ctgcaccggc cgcaccgatt
     1261 ccagccggaa ctcgtagggg ccgagcgcca cgctgtcccc cggcttgagg ttcgcgtcct
     1321 gctcgctctc gtagcccttg accagcgtca cgcccgccac gaacaccgcc acgcccaggt
     1381 gcgcgagcac catgccgtag tgggaacgcg tctggcgcag gcccttgcgc agcgtgacga
     1441 ggccggtggc ggcaatccac agggcgagcg cgaggctcaa gcccaccagc gctttccact
     1501 ggccgaaggc gaggggcagg agcagcccca ccgcaaggct cgcggccagc gcccagcgca
     1561 gcagcaccgc gagctcgggc aggctggcgc gcttccagcg cgcgagcggc cccacgccca
     1621 tcaggaacag cgccggcgcc atgagcggcg cgaacaccgc ttcgaaatac tgcggcccga
     1681 ccgagatctt gccgccgccg aaggcgtcca agagcagcgg atagagcgtg ccgagcagca
     1741 ccgagcccgc cgccgcgccg agcagcacgt tgttggcaag gaggaaggat tcgcgcgaca
     1801 ccggctcgaa gctcccgccc aggccgatgc gcgccgtgcg ccaggcgtag agcgcgagcg
     1861 cgccgccaat ggtggcggcg aacaggccga ggatgaagat gccgcgcttc gggtccaccg
     1921 cgaaggcgtg caccgaggtg aggacgcccg agcgcacgag gaaggtaccg agcagcgaca
     1981 ggccgaaggc gacgatggcg agcagcaccg tccagctgcg gaacgcgccg cgcttctcgg
     2041 tgacggcgag cgagtggatc agcgccgtgc cgaccagcca gggcatgaag gaggcgttct
     2101 ccaccgggtc ccagaaccac cagccgcccc agcccagctc gtaataggcc caggcgctgc
     2161 cgagcgcgat gcccagggtg aggaagcacc aggccacggt ggtccagggc cgcgaccagc
     2221 gcgcccaggc cgcgtccagc ctgcccgaga gcagcgccgc cacggcgaag gcgtaggcca
     2281 ccgagaagcc gacgtagccc atgtagagca tcggcgggtg gatcaccatc cccgggtcct
     2341 gcagcaacgg attcaggtcg cgcccgtccg ccggcgccgg gaacaggcgc tcgaaggggt
     2401 tggaagtgaa cagcaggaag gcgaggaagc ccgcgctcgc gaggcccagc acgccgagca
     2461 cgcgcgccgc catctcctcg ggcaggtgcc ggctgaacag gcccaccgcc gacatccaca
     2521 cgccgagcat caggcaccac agcaggagcg agccctcgtg gctgccccag gcggcggcga
     2581 agcggtagtg caggggcagc gcgctgttgg agttcatcgc cacgttggcg accgagaagt
     2641 cgctggtgac gaaagaatgc accaggcagc cgaaggcgaa ggcgacgagc agcgcctgcg
     2701 cgtgcgcggc gggccgcgcc acgcccatcc aggcggcgtc gccgcgcgcc gcgccggcga
     2761 gcggcaggat gccctgcacc agggcgacga cgagggcgag cgacagcgcg agctggccga
     2821 gctccgggat catggccgcc ggatcgtctt ctgcgccttg tccaccgcgt gggcggcttc
     2881 cggcggcatg tagttctcgt cgtgcttggc gagcacctcg cgcgcgcgga acacgccgtc
     2941 cgcgcccagc tggccctgcg ccaccacgcc cttgccctcg cggaacaggt ccggcagcgc
     3001 gccgcggtag accaccggga tggtcttcgc ggtgtcggtg acgaggaagc gcacctcgac
     3061 gccgtcgcgc ttcacgctgc ccttctccac catgccgccg atgcggaagg tgcgcccgag
     3121 cggcgcctcc ttggcggcga cctgggtggg cgtgaagaaa aagacgaggt tctgctcgaa
     3181 ggccgagagc accagcgcgg tggcggcgcc gagcgctgcc agcccgccga tgatggcggc
     3241 gaggcgctta tgtcttggct tcacgcttga gcagcaccac gatttcgatc agcaggcaaa
     3301 ggaaagtgac cccgtaggag ccccacacgt accacgcata gcctcccatg gcgagaaact
     3361 cgctcatgca cgctcccgga tgatcgagcg cacccgcagc atcgccgcgg cgatgctgta
     3421 catccagaac gccagtgcca tcaccagcat tccagtcagc atcgtggcgg ccatcttcgg
     3481 cgccgcggta aggctgaccg aggcgccctg gtgcagcgtg ttccaccact gcaccgagta
     3541 gtagatgatc gggatgttca ccacgccgac gatcgccagc agcgcggcgg cgcggtcggc
     3601 gcggcgcgga tcgtcgatcg aggcgcgcag cgccatgaag ccgaagtaga ggaacagcag
     3661 gatcagctcc gacatcaggc gcgcatcgcc ccacacccag taggtgcccc aggtcggccg
     3721 gccccacagc gcgccggtcc acagggcaat gaaggtgaac agcgccccgg tcggcgccag
     3781 cgccgaggcc atcatgccgg agagccgcgt gttcagcgcc agtccgagcg ccgcccagga
     3841 cgccatcacg acgtagatca acatggacat ccatgccgcc ggcacgtgga tgaagatgat
     3901 gcggtaggcc tcgccctgct gcgcgtcggt cggcgcgacg aagaagccga tgtaaagacc
     3961 gacggctgca agcagcgcgg cagcaatgcc gcaccagcgc gcaatcgttc ccgcgagcgg
     4021 gaagaacgtc tgcggcgagg cgtaccaaaa ccatctcatt cggacgaaat cctcagggct
     4081 gcggcgatcg cccagggggc aaaggccgcg gcgacgatca gcatggcgcc gagcagcatc
     4141 aactgcgcct ggccgccaag gccggcgccg gccatctcca cgcttcccgc gcccatgatc
     4201 agcaccggca catagagcgg cagcaccagc agcgcgagca gcacgccgcc gccgcgcagg
     4261 cccagcgtga gcgcggcgcc gatggcgccg atcaggctca gcaccggcgt gccaagcagc
     4321 aatgataagg caagcgtgcc gaagagcggc ggcggcaggt cgtactggag cgcgagcaac
     4381 ggcgcgatcg ccaccagcgg caggcccgcc accagccagt gcgcgagcag cttggcggcg
     4441 acgatcaccc cgagcggcgc cgcgccgagc agcatctgct ccagggtccc gtcggcgtga
     4501 tcgtcggcga acagccgcgg cagcgacagc atcgaggaga gcagcgccga cacccagatc
     4561 acgcccggcg ccaccgcgcg gagcaaattg ggctccgcgc cgacacccag gggaaacagg
     4621 ctcgccacga tgacgaagaa cagcagcgcc gtggccacgt cgccgcgccg gcgcagcgcc
     4681 agcagcaggt cgcggcgcag gatgcagcgg gccgcggcga tcatccgagc gcgaccacgc
     4741 gctgcaccgc ggcgccgatg cccgcctcct ggtgcgtggt gtagaccacg gcattgccgc
     4801 gcgcgacgtg gcccgcgatc agctcctcgg tgagccgcgt cgccgccgcg tcgagcgcgg
     4861 cgaagggctc gtcgagcagc cacagcggcg ccgctgcggc gagcagcagg cgcgccagcg
     4921 ccgcgcggcg gcgctgtccc tgcgagagcc ggcgcagcgg cacatcccca ggaaccccat
     4981 agctcgccag ggcaccttcc acatgctgcg cctgcgcatc cgcgccggcg atcgtgcagg
     5041 caatccccag gttctcggcg gccgtgagat cgcccttcac cgccggggcg tggccgaggt
     5101 acacgagctc ccgggagtat tcctcgcgca gcccgcggat cggcgtgccg cgccagcgca
     5161 cttcgccggc gtcgggcgcg agcagtccgg cgaggatctt caggagggtg gtcttgccgc
     5221 tgccgttctc cccggcgacg cgcagcagct cgcctccccg cagggcgaag tccaagtcct
     5281 tgaatagctg gcgcccctgg cgggcgctcc gcaggccgac tgcttcgagc acccgctatt
     5341 ttcgcctaga tcgggtcgag cccccgctcg gtgagcagca ggcggtgggt ctgcgcctca
     5401 cgcagcgcgc tcgccacctg cttcgcctcc agcaggccgg cgagcttgcg cggcccgcaa
     5461 tcgtccagca gggcgcggct caccttgagc aggtgcaccg gctcgcgcgg cttcttcagc
     5521 aggaaggaat gcgcctcgtg gtcgctgtcc cagcgctcgg cgagcgccca gtccggaaac
     5581 acctggtgca ggtatttccg tacggcctcc agacgctcct ccgcgatcct cgccatggcc
     5641 ccccacgcga cagacaccga cgcgggcact gtactcccat tccgtgaacg gcggaatcta
     5701 gttcaggtcg agcgcgcgct gcttgagcgc cgagagcggc accacctcca gcacgctttc
     5761 gtgggtggct tccagcacca cctgcggcgc ggcgccggcg cgccaggcct ccgcccatct
     5821 caaaacgtga aggcaccagc ggtcgcccgc ctgcaggccg cgaaagcgca tctccgggcg
     5881 cggcgtcacc aggtcgttgc ccttcgccac gctgaaatcg aggaactcct gcgtcacctt
     5941 gacgcacacc aggtgcgcca cgccgtcggc gccgtgcgtg gcgcagctgc cgtcgcggaa
     6001 atagccggtg agcggcgaga agctgcaggc catcagctcg ccgccgagga cgttcttctc
     6061 cgcgctcacg cggcgtgctc ctcgaggtag cgcttgagcg ccacggcatc cgcgtcgatc
     6121 agctcgcagc gctgcggcag ggcctccagc cccgcgaagc gcgccggccg ctccggttcc
     6181 cgcccgagcg cctcgacgat ggtggaggag aacttggccg gcagcgcggt ctccaggcag
     6241 accaggggca cccccggccg ccggtgcgcg aggccgactt tcacgccgtc ggcggtgtgc
     6301 gggtcgatga cgacgccgaa cttcgcatgc gccatgcgga tggtggccag gcggtcggcg
     6361 tgcgtcgact ggcccgaggc gaagccgggc ccgggcggga ggcgaaattc tccgctcttg
     6421 tccagctccg cccacagcgc gcgcacgcgc gcgccatcgc ggccgacgag gtcgaacacg
     6481 tagcgctcga agttggaggc cctggagatg tccatcgacg ggctggaggt ggcctgcgcc
     6541 gcgcggcgca cgcggtagcg gccgctgcgg aagaattcat ccagcacgtc gttctcgttg
     6601 gtggcgagga ccaggcgccc caccggcagg cccatggcgc gcgccacgta gccggcgtgg
     6661 atgttgccga aattgcccga gggcacggcg acgtccacct gctccgcgtc cgagcgcgtg
     6721 gcgcggaagt agccccagaa gtagtacacc acctgcgccg cgacgcgcgc ccagttgatg
     6781 gagttcaccg cgccgatgcg gtggcgcgcc ttgaacgcgg catcggcgtt cacctgcttc
     6841 accagcgcct ggcagtcgtc gaagctgccc tgcaccgcca ggttgtggat gttggcgtcc
     6901 tgcagcgcgt acatctgcgc cgcctggaac gcgctcatgc gcccgtgcgg cgagagcatg
     6961 aacacctgca cgccgcgctt gccgcgcagg gcgtgctcgg ccgccgagcc ggtgtcgccg
     7021 gaagtcgcgc cgaggacgtt gaggctcgcc ccgcgcgcgc cgagcacctg ctcgaagaga
     7081 ttccccagca gctgcagcgc catgtccttg aaggcgagcg tggggccgtt cgacaggcaa
     7141 agcaggtgca ggcccggctc gagcgtcttc agcggcgcga tgtccccgct gccgaaggcc
     7201 tcgcgcgtgt aggtcctgcg cgcgagcgca tcgagcccgg gcacgtcgtc catgaacagg
     7261 cgcaggaccg cggcggcgag atcgggatag cgcaggccgc gccaggcggc gaggtccgcg
     7321 cgcgggtagg cctcgggcac gtacagcccg ccgtcgcccg ccaggccctc gagcaggatg
     7381 tcggagaagc gctgcggctc ggcgccgccg cgggtgctga ggtatcgcaa gcggctagcg
     7441 cttttcgctc ttttgctgca gctggttgaa cctgcggatc tgctcgggcg tcgccttgcg
     7501 gtgcgccttc tgcgccttca ccggcacggg cttgcgcgcc tgcttctgcg ccggcgccgg
     7561 cgattgcgcc caggccggcg caaggcagca ggcgagcgcc agcgccgccc agggcctcac
     7621 agcagctcct ccaggcggat gcgcgtcacc ttgccgagca ccgtcggcag gcgctcgatg
     7681 cgcgccatgg cggcatcgac gttcttctcg atcgcgcggt gggtgagcat gacgatgtcg
     7741 acgcgctgct cgccctcgcc gggctccttc tgcaccatcg cgtcgatcga gatcctcgag
     7801 tcggccagga tgcgcgtgat gcgcgccagc accccgggcc ggtcgagcac gcgcatgcgc
     7861 aggtagtacg aggtctcgac ctcgccgatc ggcaggatcg gctcgtccga catctggtcg
     7921 tgctggaacg caaggtgcgg cacgcggtgc tccgggtccg ccgtgatcag gcgcgtcacg
     7981 tccaccaggt cggccaccac tgcgctcgcg gtcggcaggc tgcccgcgcc cgcgccgtag
     8041 tacagggtcg cgccgaccgc gtcgcccttc accaggatgg cgttcatcac gccctccacg
     8101 ttggcgatca ggcgccgcgc cggcacgagc gtcgggtgca cgcgcagctc gatgccccgc
     8161 cgcgcgcggc gcgtgatgcc gagcagcttg atgcggtagc cgagctcctc ggcgtagccg
     8221 atgtcggcct gcgtgagccc ggagatgccc tcggtgtagg ccttcgggaa ctgcatcggg
     8281 atgccgaagg cgagcgccga gaggatggtg agcttgtgcg cggcatcgat gccttcgatg
     8341 tcgaaagtcg ggtcggcctc ggcgtagccc ttggcctgcg cctcggccag caccgtggcg
     8401 aatggcgaac ccctgtcgcg catctccgac aggatgaagt tcgaagtgcc gttgatgatg
     8461 ccggcgatcc actcgatgcg gttggccgcc aggccctcgc gcagcgcctt gatgatcggg
     8521 atgccgccgc cgacggaggc ctcgaaggcc accatcacgc cctttttctg cgccgcggcg
     8581 aagatgcggt tgccgtgctt ggcgagcagc gccttgttgg cggtgaccac gtgcttgccg
     8641 tgcgcgatcg cctccagcac caggtccttc gcgatgccgg tgccgccgat cagctccacc
     8701 acgatgtcga tgtccggcgc gcgcaccact tcccaggcgt tgccggagac cctggcgcgg
     8761 ccgccgacca gcttgcgcgc cttcttctgg tccttgtcgg cgacgcgcgc gatgcggatc
     8821 gcgcggccgg cgcggcggcg gatctcgccc gcattgcggc tgaggacctg ccaggtgccg
     8881 ctgccgaccg tgccgatgcc gagcagcccc acggcaatcg gtttcatgcc gctccgcgca
     8941 gcaggccgtc cttcttgaac atctcgcgga tgccgcgcag cgcctggcgc aggcggtgtt
     9001 cgttctcgat cagggcgatg cgcacgtggc cgtcgccgta ctcgccgaag ccgatgcccg
     9061 gcgagaccgc gatcttcgct tcctgcatca ggcgcttggt gaattcgatg gagcccatct
     9121 tcgcgtagaa gtcgggaatc ttcgcccaga tgtacatcga ggccttcggg gcctccacca
     9181 tccagccgat gtcgtgcagc cccttcacca tcacgtcgcg gcggcgctgg tagttctgcc
     9241 ggatctcccc gacgcagtcc tgcggcccct ccagcgcgac gatcgaggcg acctgcaccg
     9301 gcgtgaagct gccgtagtcg tggtagctct tgatgcgcgc cagcgcgtgc accagctccg
     9361 cgttgcccac catgaagccg atgcgccagc cggccatgtt gtagctcttg gacatggtga
     9421 agaactccac cgccacgtcg cgcgcgcccg gcacctgcag gatcgagggc gccttccagc
     9481 cgtcgaaggt gatgtcggcg taggcgaggt cgtgcaccac gaggatgtcg tgctgcttcg
     9541 ccagcgcgat cacgcgctcg aagaaatcca gctccacgca catcgccgtc gggttggacg
     9601 ggaagccgag gatcagcatc ttcggcttcg ggatcagctc gccgatggcg cgctccagct
     9661 cggcgaagaa atccaccccc ggcgtcatgc gcaccgagcg gatgtcggcg ccggcgatga
     9721 tcgcgccgta gatgtggatc gggtaggagg gattgggcac cagcaccgtg tcgccgcgat
     9781 cgagcgtcgc cagcatcagg tgcgcgaggc cttccttgga gccgatggtg acgatggctt
     9841 ccttgtccat gtccagcgtc accgcgtagc gccgctggta ccagtcgcag atggcgcggc
     9901 gcaggcgcgg aatgcccctg gagaccgagt agccgtgggt atcggggcgc cgcgaggcct
     9961 cgaccagctt gtcgacgatg tgctgcggcg tgggcccgtc gggattgccc atcgacaggt
    10021 cgatgatgtc ctcgccgcgg cggcgcgccg ccatcttcaa ctcgttggtg atgttgaaga
    10081 cgtagggcgg aagacgcttg atccttgaaa actctcgcat tgtagaattc tagcagtgaa
    10141 actccacgct tccgccctca cccggctgca ccgcttcacc ggctacggcg cgggctacgt
    10201 gatggtgaac gggacgcgcc acgagtcgag cctgctggtg catcccgagc gcatcgagcc
    10261 ctggccggtg gcgggcttcg ccgcgctggc cgaggcgcac ttcgcgcgca tcgccgagct
    10321 cgggcccgag gtggtcctgc tcggcaccgg cgaccggctg cgctttcccc accccgggct
    10381 ggtcgcgccg ctcgcgcgcg cgcgcatcgg cttcgaggcg atggacctgc aggccgcctg
    10441 ccgcacctac aacatcctga tggccgagga gcgcaacgtg atcgcggcat tgctgttcgc
    10501 atgagcctcg tcagcttcgc gtacgtcgcg ctcgccgtgg tgctcggcac cggcgcgcag
    10561 ctgctgctca aggccggcac caacgccacg ccggtcgggc tcgccctggc gctcgacccg
    10621 cgcgtcctcg gcggcgtcgc ctgctatggc gtgagcctgg tggcctggat cctggcgctg
    10681 tccaggaccc cggtcagcgt cgcctacccg atggtgtcga tcggcttcgc gctgaacgcc
    10741 ctgctcgcct ggtggctgct gggcgaggcg ctgaacgcgc agcgcatcgc cggcatcgca
    10801 gtgatcatgc tgggggtgtg gctggtggcg cgatcgtaga tggccctgct tcccggcttc
    10861 aagaccaggc gcatccgcac ttccggcgcc a
//
LOCUS       DASRIE010000044         9304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2918412, whole genome shotgun sequence.
ACCESSION   DASRIE010000044 DASRIE010000000
VERSION     DASRIE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918412"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFB53_01940"
     CDS             <1..1185
                     /locus_tag="VFB53_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85522.1"
                     /translation="VVKQIEPHDSVERYEALGVECLHGEAMIASPWTVEIQMQGGGKK
                     TLSTRSIVIAAGGRPFVPPIPGIEDTGYYTSDTLWDLRVLPRRLVVLGGGPIGCELAQ
                     AFQRLGAQVTQVEMLPRLLVREDPEIAELVAQRFREEGVDLRLAHKAKEFRAEGGRKV
                     LICEHEGRDLAIEFDALLVAVGRVANTAGYGLEALGIPTGPGRTVQTNEYLQTAYPNI
                     YACGDVAGPYQFTHTAAHQAWYAAVNALFGGLKKFRADYAVIPWATFTDPEVARVGLN
                     ETEAKERGIACEVTRYGLDELDRAIADEEAHGVVKVLTVPGKDRILGVTIAGEHAGDL
                     IAEFVAAMRHGIGLNKILGTIHIYPTLAEANKYAAGVWKKAHAPQRLLRHVERFHAWL
                     RG"
     gene            1170..1838
                     /locus_tag="VFB53_01945"
     CDS             1170..1838
                     /locus_tag="VFB53_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN85523.1"
                     /translation="MAARLSVVVPVLDEAAGIEACLAALAPLRARGHEVIVVDGGSAD
                     ATPRLAAPLADRVLAAPRGRAAQMNAGAAAATGEALLFLHADTVLPPEADRAVLAALE
                     SRAWGRFDVRIASRDPRLALVGWCMNWRSRLSGIATGDQAIFVRRAAFPGFPPIALME
                     DVAFSRAMKRVSAPACLRQRVSTSARRWERRGVLATVLLMWRLRLAYWLGADPEALAR
                     RYAR"
     gene            1840..2385
                     /locus_tag="VFB53_01950"
     CDS             1840..2385
                     /locus_tag="VFB53_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZN85524.1"
                     /translation="MIVFAREPRAGRVKTRLIPLLGEQGAARLHARLVARALATARAA
                     GIGEVELLWARRQRGADLGERMQHALARGLRRAPRAILIGADCPVLRARDLRRAARWL
                     AGGADAVFAPAEDGGYALIGLRRVSPRLFAGVAWGGAQVMAQTRERLAALGWRWRELP
                     AVWDVDRPEDYRRLQRAGLLQ"
     gene            complement(2359..2955)
                     /locus_tag="VFB53_01955"
     CDS             complement(2359..2955)
                     /locus_tag="VFB53_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZN85525.1"
                     /translation="MRTLTWLAALLIASCGDAPKLAPLAPDAVVLAFGDSLTYGTGAA
                     EADSYPAQLEKLIGRRVVRAGVPGEVSARALERLPGELEEHRPALLLLCIGGNDFLRN
                     LGKAQVAENVRRMVAMARAQGAQVLLIGTPEKGLLVTPPAFYAEIAEQAGMPYEGKVI
                     GEILRNSELKSDPIHPNAKGYRLIAERVAALLQKAGAL"
     gene            3009..3677
                     /locus_tag="VFB53_01960"
     CDS             3009..3677
                     /locus_tag="VFB53_01960"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN85526.1"
                     /translation="MTIRNPRDFWAGVMFVAFGLAFVLIAAGTPAFLDPVLGALKVPG
                     YQIGSAVRMGPAYFPIVLGGLLATLGAVILARGFVSGRSGEEVRLTLPFNLLDLVIGL
                     GTFALLLAYGDRVGLSLDGAMLAAAVVVSLLAVRWRPSAKPLVLILAGCLAFGYTLKP
                     LGLVGASLLLIFISALGGHEFKWREVAILFVVLVVFSVLVFVKGLTLPFPVCPGFIDN
                     CPIR"
     gene            3687..5237
                     /locus_tag="VFB53_01965"
     CDS             3687..5237
                     /locus_tag="VFB53_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN85527.1"
                     /translation="MEGILGNLGIGFVEVFKLQAVTIAGVSMTLPFNILMCLIGVMLG
                     TLIGVLPGVGPVATIAMLLPVTFNLNPIAALIMLAGIYYGAQYGGSTSAILVNLPGES
                     ASVVTCLDGYQMARRGRAGPALATAAIGSFVAGCVATLVVALFAPPLAEVALKFGPSE
                     YFSLMVLGLVAATVLAHGSLVKAIAMVLFGLLLGLIGTDVNSGVLRFAFGVSELADGI
                     GFTTVAMGMFGLAEIIANLEQKGEREVFTGKVTHLMPTREDFKRIWAPILRGTALGSM
                     LGILPGGGALLSSFGAYTLEKKISKNSHEFGKGAIEGVAAPESANNAGAQTSFIPLLT
                     LGIPGNAVMALMLGAMMIQGIAPGPQVMTERPQLFWGMIASMWVGNLMLVVLNLPLIG
                     MWIKLLTVPYRILYPSILLFMCVGVYSLSNNPFDVLLMALFGLLGYICVKLECEPAPM
                     ILGFILGPLMEENLRRAMLLSRGDPLVFFQKPISAGFLVVSIILLVIVMLPNIRKKRE
                     EVFVEEAT"
     gene            complement(5242..5628)
                     /locus_tag="VFB53_01970"
     CDS             complement(5242..5628)
                     /locus_tag="VFB53_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZN85528.1"
                     /translation="MAEIKIGNVEALGTPLGQYSHLARVKNAQETLYIAGMLAPGEDF
                     DAQCSGVFRQIETALRSAGAGWGNVAQFTTYLVHSQDIPKFMAWRLREFPRMFPDGKY
                     PPNTLLMIDRLVGEQFLIEVQTIAAL"
     gene            complement(5642..6058)
                     /locus_tag="VFB53_01975"
     CDS             complement(5642..6058)
                     /locus_tag="VFB53_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN85529.1"
                     /translation="MSAKHAAAPRHRLLIVDDEESTRLLIARLLAAGTKCEAQLAGTC
                     EQALRLAGDYAYDAILLDLLMPGIGGYEVLREIRRASANTATPILIVSVLSDQETKDR
                     CKAAGADGFLVKPVERRALLEAVKALLAARGKPKKT"
     gene            complement(6055..6777)
                     /locus_tag="VFB53_01980"
     CDS             complement(6055..6777)
                     /locus_tag="VFB53_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZN85530.1"
                     /translation="MRSRAPAVKALVFDVFGTVVDWRSSIIREGRLLGRRKKLKVDWE
                     AFADAWRAGYRPAMARVRSGELPWTKIDALHRMILDELLVRFAIRGLSEEEIDHLNRA
                     WHRLMPWPDAKAGLAKLKRRHVIATLSNGNLALLTNMAKHAGLPWDCILSAEVVRHYK
                     PDPETYLGAAELLEVKPAELMMVAAHKDDLHAARACGLKTAFVPRPLEHGPARKLDVA
                     REPAFDLHARDFNDLARQLAGR"
     gene            complement(6788..7531)
                     /locus_tag="VFB53_01985"
     CDS             complement(6788..7531)
                     /locus_tag="VFB53_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZN85531.1"
                     /translation="MNWRGKPQLQERLAAEYALGTLRGLARQRFERWMRADAALARAA
                     AEWQARLAPLAAGVRPVAPPERLWRQIQARLGAAAPGSLWENLAFWRGLGLAASGAAA
                     VLLAAVALVSPQAPVPAPAPVVLKVPSTEMAEVYLAVLSDPKTNKPLLLVTAGRHADQ
                     MQVRTLDPSIRVSGKDLELWALPAGGVPTSLGLVGETERSMVKLAAVASRSLANVPTL
                     AISLEPKGGSPTGRATGPVLAVGPCIKFW"
     gene            complement(7528..8076)
                     /locus_tag="VFB53_01990"
     CDS             complement(7528..8076)
                     /locus_tag="VFB53_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN85532.1"
                     /translation="MAQDDLAGLLAACARRDRAAFERLYRATAPKLFGVALRILRRED
                     WAEDVLQECYLRIWDHAPAYRAGLAAPMTWMTSIVRNRCLDWLRRPRLEVIDEEGELI
                     EAAPSADPGPLEELESSSDARALKRCLDALEAKQRQAIALAYYDGLSHAELASQLRAP
                     LGTVKTWVRRGLLRLKACLETA"
     gene            complement(8079..8633)
                     /locus_tag="VFB53_01995"
     CDS             complement(8079..8633)
                     /locus_tag="VFB53_01995"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZN85533.1"
                     /translation="MGNRLSKITTRTGDSGDTGLGDGARVGKDSLRIQLLGDVDELNS
                     CLGLLLAEKPPRDVHIALLRVQQDLFDLGGEVCIPGHSMITAAQVARLEALTQRHNAK
                     LKPLKEFILPGGTRAAAAAHLARTVCRRAERSLVALGRREAVGERARQYLNRLSDLLF
                     VLGRALNRAAKRGDVLWQHERKKR"
     gene            8681..9091
                     /locus_tag="VFB53_02000"
     CDS             8681..9091
                     /locus_tag="VFB53_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85534.1"
                     /translation="MASVRKASYYSMKVPQRPGSGAQLLAALKAAKVNLLAFTGFPEG
                     GGAQVDFVPQDNAKFTKVARKAKLRISSRKTVFLAQGADKVGALTQLLARLAKAKINL
                     VALSAVTAGSRRFGMLFWVKPKDVAKASRVLKAR"
     gene            complement(9104..>9304)
                     /locus_tag="VFB53_02005"
     CDS             complement(9104..>9304)
                     /locus_tag="VFB53_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN85535.1"
                     /translation="VRASSLDWTFAYPTLLTNGPRTGKYRAGERLELSGMPKISRADV
                     ADFVLKQLQDRSWSRKRPVISY"
BASE COUNT         1225 a         3265 c         3360 g         1454 t
ORIGIN      
        1 gtcgttaaac agatagagcc gcacgactcc gttgagcgct acgaggcgct gggcgtggaa
       61 tgcctccacg gggaagcgat gatcgcttcc ccgtggaccg tggaaattca aatgcaaggc
      121 ggtgggaaga aaacattgag cacgaggtcg atcgtcattg cggccggcgg ccgtcccttc
      181 gtgccgccga ttcccggcat cgaggacacc ggctactaca cctccgacac gctgtgggac
      241 ctgcgcgtgc tgccgcggcg gctggtggtg ctgggcggcg gtccgatcgg ctgcgagctg
      301 gcgcaggcct tccagcgcct gggcgcgcag gtgacgcagg tggagatgct gccgcggctg
      361 ctggtgcgcg aggacccgga gatcgcggag ctggtggcgc agcgcttccg cgaggagggc
      421 gtcgacctgc gcctcgcgca caaggcgaaa gagttccgcg cagagggcgg gcgcaaggtg
      481 ctgatctgcg agcacgaggg gcgcgacctg gcgatcgagt tcgacgcgct gctcgtcgcg
      541 gtgggccgcg tggcgaacac cgcgggctac gggctggagg cgctgggcat cccgacgggc
      601 ccggggcgca ccgtgcagac caacgaatac ctgcagacgg cgtatcccaa catctacgcc
      661 tgcggcgacg tcgccgggcc ctaccagttc acgcacaccg ccgcgcacca ggcctggtac
      721 gcggcggtca acgcgctgtt cggcggcctg aagaaattcc gcgccgacta cgcggtgatt
      781 ccctgggcca ccttcaccga cccggaagtg gcgcgcgtcg gcctcaatga gaccgaggcg
      841 aaggagcggg gcatcgcctg cgaagtgacg cgctacgggc tggacgaact ggaccgcgcc
      901 atcgccgacg aggaggcgca cggggtggtg aaggtgctca ccgtgccggg gaaggaccgc
      961 atcctcgggg tcacgatcgc cggggagcac gccggcgacc tgatcgccga attcgtcgcc
     1021 gcgatgcgcc acggcatcgg cctgaacaag atcctcggca ccatccacat ctatccgacg
     1081 ctcgccgagg ccaacaagta cgccgccggg gtgtggaaga aggcgcacgc gccgcagcgc
     1141 ctgctgcgcc acgtggagcg attccacgcg tggctgcgcg gctgagcgtc gtcgtcccgg
     1201 tcctcgacga ggcggcgggg atcgaagcct gcctcgccgc cctcgcgccg ctgcgcgcgc
     1261 gcggccacga ggtgatcgtg gtcgatggcg gcagcgccga tgccacgccg cggctcgcgg
     1321 cgccgctcgc cgaccgggtg ctcgccgcgc cgcgcgggcg cgccgcgcag atgaacgccg
     1381 gcgcggccgc cgcgacgggc gaggcgctgc tgttcctgca tgccgatacg gtcctgccgc
     1441 cggaggcgga ccgcgcggtc ctggcggcgc tcgagtcgcg cgcctggggc cgcttcgacg
     1501 tgcgcatcgc ctcgcgcgac ccgcgcctcg cgctggtcgg ctggtgcatg aactggcgct
     1561 cgcgcctgag cggcatcgcc accggcgacc aggcgatctt cgtgcggcgc gccgcgttcc
     1621 cgggatttcc gccgatcgcg ctgatggagg acgtcgcctt cagccgggcg atgaagcgcg
     1681 tctcggcgcc cgcctgcctg cgccagcgcg tgtccacctc ggcgcggcgc tgggagcgcc
     1741 gcggcgtgct cgccacggtg ctcctcatgt ggcgcctgcg cctcgcctac tggctgggcg
     1801 ccgacccgga agcgctggcc cgccgctatg cgcgctaggg tgatcgtgtt cgcccgcgag
     1861 ccgcgcgccg gccgggtgaa gacgcgcctc atcccgctgc tcggcgagca gggcgccgcg
     1921 cggctgcacg cgcggctggt cgcgcgcgcg ctcgccacgg cgcgcgccgc cggcatcggc
     1981 gaggtggagc tcctctgggc gcgccgccag cgcggcgcgg acctcggcga gcgcatgcag
     2041 catgcgctcg cgcgggggct gcggcgcgcg ccgcgcgcga tcctcatcgg cgcggactgc
     2101 ccggtgctgc gcgcgcgcga cctgcgccgc gccgcgcgct ggctcgccgg cggcgccgac
     2161 gcggtgttcg cgccggccga ggacggcggc tacgcgctca tcgggctgcg gcgcgtttcg
     2221 ccgcgcctgt tcgcgggcgt cgcctggggc ggggcgcagg tgatggcgca gacgcgcgag
     2281 cgcctggcgg cgctcggctg gcgctggcgc gagctgcccg cggtctggga cgtggaccgg
     2341 cccgaggact accgccggct acagcgcgcc ggccttctgc agtagcgccg ccacccgctc
     2401 ggcgatcagc cggtagccct tggcgttggg gtggatgggg tcggacttga gctccgagtt
     2461 gcgcaggatc tcgccgatca ccttgccctc gtatggcatg ccggcctgct ccgcgatctc
     2521 ggcgtagaag gcgggcggcg tcaccagcag ccctttctcc ggcgtgccga tcagcagcac
     2581 ctgggcgcct tgcgccctgg ccatcgccac catgcgccgc acgttctcgg ccacctgcgc
     2641 cttgccgagg ttgcgcagga aatcgttgcc gccgatgcac aggaggagca gggcgggccg
     2701 gtgctcctcc agctccccgg gcaggcgctc gagcgcgcgc gcgctcacct cgccgggcac
     2761 gccggcgcgc accaccctgc gcccgatcag cttttccagc tgcgccgggt agctgtcggc
     2821 ctcggcggcg ccggtgccgt aagtgaggct gtcgccgaag gcgagcacca ccgcgtcggg
     2881 ggcaagcggc gcgagcttcg gcgcgtcgcc gcaggaagcg atcagcagcg ccgcgagcca
     2941 ggtcagagtg cgcatcggcc tgcatgctac actcggcgcg actggacccg gaggagggcg
     3001 gcggcgcgat gacgatcagg aaccccaggg acttctgggc aggagtcatg ttcgtcgctt
     3061 tcggcctggc gttcgtcctc atcgccgccg gcacgccggc cttcctcgac ccggtcctcg
     3121 gcgcattgaa ggttcccggc taccagatcg gcagcgcggt gcgcatgggg ccggcgtact
     3181 tccccatcgt gctcggcggc ctgctggcga cgctcggcgc ggtgatcctc gcgcgcggct
     3241 tcgtctccgg gcgcagcggc gaggaagtgc gcctcaccct gccgttcaac ctgctcgacc
     3301 tggtgatcgg gctggggacc ttcgcgctgc tgctggccta cggcgaccgc gtcggcctgt
     3361 ccctcgacgg cgccatgctg gcggcggcgg tcgtcgtgtc gctgctcgcg gtgcgctggc
     3421 gcccgagcgc caagccgctg gtgctgatcc tcgccggctg cctcgccttc ggctacaccc
     3481 tgaagccgct cggcctggtc ggcgcctcgc tcctcctgat cttcatcagc gccctgggcg
     3541 gccacgagtt caagtggcgc gaggtggcga tcctgttcgt ggtgctggtc gtgttctcgg
     3601 tgctggtgtt cgtcaagggc ctgacgctgc ccttcccggt gtgccccggc ttcatcgaca
     3661 actgcccgat ccgctagccg ccgaccatgg aaggcatcct cggcaacctc ggcatcggct
     3721 tcgtcgaagt cttcaagctg caggcggtga ccatcgccgg cgtcagcatg acgctgccgt
     3781 tcaacatcct gatgtgcctg atcggcgtga tgctcggcac cctgatcggc gtgctgcccg
     3841 gcgtcggacc ggtggcgacc atcgccatgc tgctgccggt gaccttcaac ctgaacccca
     3901 tcgcggccct catcatgctg gccgggatct actacggcgc gcagtacggc ggctcgacca
     3961 gcgcgatcct cgtcaacctg ccgggagagt cggcttcggt ggtgacctgc ctggacggct
     4021 accagatggc gcgccgcggg cgcgcggggc ccgcgctcgc caccgccgcc atcggctcgt
     4081 tcgtcgctgg ctgcgtggcc accctggtgg tggcgctgtt cgcgccgccg ctcgccgagg
     4141 tggcgctcaa gttcgggccc tcggagtact tctccctgat ggtgctgggc ctggtggcgg
     4201 ccaccgttct ggcgcacggc tcgctcgtca aggcgatcgc catggtgctg ttcggcctgc
     4261 tgctcgggct gatcggcacc gacgtgaact cgggcgtgct gcgcttcgcc ttcggcgtct
     4321 cggagctcgc cgacggcatc ggcttcacca cggtggcgat gggcatgttc ggcctcgccg
     4381 agatcatcgc caacctggag cagaagggcg agcgcgaggt gtttaccggg aaagtgaccc
     4441 acctgatgcc gaccagggag gatttcaagc gtatctgggc gccgatcctg cgcggcacgg
     4501 cgctcggctc gatgctcggc atcctgcccg ggggcggcgc gctgctgtcc tcgttcggcg
     4561 cctacacgct ggagaagaag atctcgaaga actcgcacga gttcggcaag ggcgcgatcg
     4621 agggcgtcgc cgcgcccgag tcggcgaaca acgccggcgc gcagacctcc ttcatcccgc
     4681 tgctcacgct cggcatcccg ggcaacgcgg tgatggcgct gatgctgggc gcgatgatga
     4741 tccagggcat cgcgccgggc ccgcaggtga tgaccgagag gccgcagctg ttctggggca
     4801 tgatcgcctc gatgtgggtg ggcaacctga tgctggtggt gctcaacctg ccgctcatcg
     4861 gcatgtggat caagctgctc accgtgccct accgcatcct gtacccgtcg atcctgctgt
     4921 tcatgtgcgt gggcgtgtac agcctgtcca acaacccctt cgacgtgctg ctgatggcgc
     4981 tgttcggcct gctgggctac atctgcgtga agctcgagtg cgagccggcg ccgatgatcc
     5041 tcggcttcat cctcgggccg ctgatggagg agaacctgcg ccgcgccatg ctgctgtcgc
     5101 gcggcgaccc gctggtgttc ttccagaagc cgatcagcgc cggcttcctg gtggtgtcga
     5161 tcatcctgct ggtgatcgtg atgctgccca acatccgcaa gaagcgcgaa gaggtgttcg
     5221 tcgaggaggc gacctagcgg cctacagcgc cgcgatggtc tgcacctcga tcaggaactg
     5281 ctcgcccacc aggcgatcga tcatcagcag cgtgttcggc gggtacttgc cgtcggggaa
     5341 catccgcggg aactcgcgca gccgccaggc catgaacttc gggatgtcct gcgagtgcac
     5401 caggtaggtg gtgaactgcg cgacgttgcc ccagccggcg cccgccgacc tgagcgcggt
     5461 ctcgatctgc cggaacaccc cgctgcactg cgcgtcgaaa tcctcccccg gcgccagcat
     5521 ccccgcgatg tagagcgtct cctgcgcgtt cttcacccgc gccaggtgcg agtactgccc
     5581 gagcggcgtg cccagggcct ccacgttgcc gatcttgatt tccgccatgg ctcccgcctc
     5641 ctcaggtctt cttcggtttg ccgcgcgcgg cgagcagtgc cttcactgcc tccagcagcg
     5701 cgcggcgctc gaccggcttc accaggaagc cgtccgcgcc cgccgccttg cagcggtcct
     5761 tggtctcctg gtccgagagc accgagacga tgaggatcgg cgtcgccgta ttggccgagg
     5821 cgcggcggat ctcgcgcagc acctcgtagc cgccgattcc cggcatcagg aggtccagca
     5881 ggatcgcgtc gtaggcgtag tcgcccgcca ggcgcagcgc ctgctcgcag gtgccggcga
     5941 gctgcgcttc gcacttcgtg ccggcggcga gcagccgcgc gatcagcaag cgcgtggatt
     6001 cctcgtcgtc gacgatcagc aggcggtggc gcggcgcggc cgcatgcttc gcgctcatcg
     6061 gcccgccagc tgccgggcga ggtcgttgaa atcgcgcgcg tgcaggtcga aggccggctc
     6121 gcgcgcgacg tcgagcttcc tcgccgggcc gtgttccagc ggccgcggca cgaacgcggt
     6181 cttcaggccg caggcgcgcg ccgcgtgcag gtcgtccttg tgcgccgcca ccatcatcag
     6241 ctcggcgggc ttcacctcca gcagctcggc ggcgccgagg taggtttccg ggtcgggctt
     6301 gtagtggcgc accacctcgg cagacaggat gcagtcccag ggcaggccgg cgtgtttcgc
     6361 catgttggtg agcagcgcca ggttgccgtt ggacagggtg gcgatgacgt gccggcgctt
     6421 gagcttcgcc agccccgcct tggcgtccgg ccagggcatc agccggtgcc aggcgcggtt
     6481 gaggtggtcg atctcctctt ccgagagccc gcggatggcg aaccgcacga ggagctcgtc
     6541 gaggatcatg cggtgcaggg cgtcgatctt ggtccagggc agttcgcccg agcgcacgcg
     6601 cgccatcgcc gggcggtagc ccgcgcgcca ggcgtcggcg aaggcctccc agtccacctt
     6661 gagttttttc ctgcggccga ggagtcttcc ctcgcggatg atggagctgc gccaatccac
     6721 caccgtgccg aagacgtcga acacgagggc tttgacggcg ggggcgcggg atctcattcc
     6781 cgcattatta ccagaacttg atgcagggac ccacggccag caccggcccg gtggcgcgcc
     6841 cggtgggcga gccgcccttg ggctccaggc tgatggcgag ggtgggcaca ttggcgagcg
     6901 agcggctggc caccgcggcg agcttgacca tcgagcgctc ggtctcgccc accagcccga
     6961 gcgaggtcgg cacgccgccc gcgggcagcg cccagagctc caggtccttg cccgagacgc
     7021 ggatcgacgg gtcgagcgtg cgcacctgca tctggtcggc gtgccggccg gcggtcacga
     7081 gcagcagcgg cttgttggtc tttggatcgg acaggaccgc gaggtaaacc tcggccattt
     7141 ccgtcgaagg caccttcagc accaccggcg cgggggcggg caccggcgcc tgcggcgaga
     7201 cgagcgccac cgcggcgagc agcacggcgg cggcgccgct cgccgcgagg cccaggccgc
     7261 gccagaaggc gaggttctcc cacaggctac ccggcgccgc ggcgcccagg cgcgcctgga
     7321 tctggcgcca gagccgctcg ggcggcgcga ccggccgcac gccggcggcg agcggggcga
     7381 gccgcgcctg ccattcggcc gctgcgcgcg cgagcgcggc gtccgcgcgc atccagcgct
     7441 cgaagcgctg ccgcgcgagg ccgcgcaggg tgccgagcgc gtactcggcg gcgaggcgct
     7501 cctgcagctg cggcttgccg cgccagttca tgccgtctcc aggcaggcct tcaggcgcag
     7561 caggccccgg cgcacccagg tcttcaccgt gccgaggggc gcgcgcagct ggctcgccag
     7621 ctccgcgtgc gacagcccgt cgtagtaggc gagcgcgatg gcctggcgct gcttggcttc
     7681 cagcgcgtcc aggcagcgct tgagggcgcg cgcgtccgag ctcgattcga gctcctcgag
     7741 cgggccggga tcggcgctcg gcgcggcctc gatcagctcg ccttcctcgt cgatcacctc
     7801 gagcctgggc cggcgcagcc agtcgaggca gcggttgcgc acgatgctcg tcatccaggt
     7861 catcggcgcg gcgagccctg cgcggtacgc cggcgcgtgg tcccagatgc gcaggtagca
     7921 ttcctgcagc acgtcttcgg cccagtcctc acgcctcaat atacgcagcg cgacgccgaa
     7981 cagtttcggc gcggtggcgc ggtacaggcg ctcgaaggcg gcgcggtcgc gccgcgcgca
     8041 agccgccagc aggcccgcca ggtcgtcctg cgccatgctc agcgtttctt gcgctcgtgc
     8101 tgccagagca cgtcgccgcg cttcgctgcg cggttcagcg ccctgccgag gacgaagagg
     8161 aggtcggaga ggcggttcag gtactggcgc gcgcgctcgc ccacggcttc cctgcgtccc
     8221 agggccacca ggctcctctc ggcccggcgg cacacggtgc gcgcgaggtg cgcggcggcc
     8281 gcggcgcgcg tgccgcccgg caggatgaat tccttcaggg gcttgagctt cgcgttgtgt
     8341 cgctgagtca gcgcctccag gcgcgcgacc tgcgccgcgg tgatcatgct gtggcccggg
     8401 atgcagacct cgccgccgag gtcgaacagg tcctgctgca cgcgcagcag ggcgatgtgg
     8461 acgtccctgg gcggcttttc cgcgagcagc aggccgaggc aggagttgag ctcgtccacg
     8521 tcgcccagca gctggatgcg caggctgtcc ttgcccacgc gcgcgccgtc gcccaggccg
     8581 gtgtcgcccg agtcgccggt gcgggtggtg atcttcgata gcctgttgcc catgaggagc
     8641 gcagaattat agggctgcat cacgattcca aggaggaacc atggccagcg tgcgcaaagc
     8701 gtcctactac tcgatgaagg tcccccagcg cccgggctcc ggcgcccagc tgctcgccgc
     8761 gctgaaggcg gcgaaggtca acctgctcgc gttcaccggc ttcccggaag gcggcggcgc
     8821 gcaggtggac ttcgtgccgc aggacaacgc gaagttcacc aaggtggcgc gcaaggcgaa
     8881 gctgaggatc tcttcgcgca agaccgtgtt cctcgcgcag ggcgcggaca aggtcggcgc
     8941 gctgacccag ctgctcgccc gcctggcgaa ggcgaagatc aacctggtgg cgctgagcgc
     9001 ggtgacggcg ggcagccgcc gcttcggcat gctgttctgg gtaaagccga aggacgtggc
     9061 gaaggcttcg cgggtgctga aggcgcgcta gcccgggcga gagtcagtag ctgatcaccg
     9121 ggcgcttcct cgaccagctg cggtcctgca gctgcttgag gacgaagtcg gcgacgtcgg
     9181 cgcgcgagat cttcggcatg ccggagagtt ccaggcgctc gccggcgcgg tatttcccgg
     9241 tgcgcggccc gttggtgagc agcgtcgggt aggcgaaggt ccagtccagg ctgctcgcgc
     9301 gcac
//
LOCUS       DASRIE010000045         5954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1610278, whole genome shotgun sequence.
ACCESSION   DASRIE010000045 DASRIE010000000
VERSION     DASRIE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610278"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFB53_02010"
     CDS             complement(<1..619)
                     /locus_tag="VFB53_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN85536.1"
                     /translation="MSKENARPVILAVDDAEDLLALIGKALSTEYEVKLAADGGDALT
                     LAAADPRPDLILLDVDMPGASGFEICQLLKEDPATAHIPVIFLTGKTEAGDQVEGLDL
                     GAVDYLTKPINGRLLMARVRIHLALANRQAELERQVAERTAQLDDARSQLIRRLARAM
                     EFHESAAVGNRVMRLGHYAKLISQAAGAPPEAAEMMMKAAPLHDIG"
     gene            804..1007
                     /locus_tag="VFB53_02015"
     CDS             804..1007
                     /locus_tag="VFB53_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN85537.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGKDLFAHFSAIQGSGFKSLKD
                     GQKVSFDVTTGPKGDQATNIKPM"
     gene            1133..1939
                     /locus_tag="VFB53_02020"
     CDS             1133..1939
                     /locus_tag="VFB53_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85538.1"
                     /translation="MSAFQGRTAFVTGGASGIGRGLCEALAARGAELTVADIDFEGAQ
                     AVAGGILRAGGKARALRLDVADGEAVTRALESAGRLDYVFNNAGLAIVGEVRDSDLAS
                     WRRIVDVNLMGVVAGSLAAYRIMARQGSGHIVNTASLAGLAGMPVGAAYAMTKAGVVM
                     FSQMLRAEGEALGVKVSAACPGFIRTPIFDNAQYLRVDKARMLKFIPLPIIALAPAVE
                     GILAGVGRNDALIVLPFYARLIWRLLRWFPFLAGPVNRKAMADFRRLRIP"
     gene            1950..2333
                     /locus_tag="VFB53_02025"
     CDS             1950..2333
                     /locus_tag="VFB53_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85539.1"
                     /translation="MTTFRKVTLYTAGDGLAKFREEDLPLPEGKPQAMLSALFPSSGY
                     QMRHSPVGFRSDWHCSPLPQWVFILQGEMEIHLRDGSSRRFRPGGHFFSTDTVPAGAA
                     FDPKLHGHWSRQAGPDALVTLFLRA"
     gene            2345..3967
                     /locus_tag="VFB53_02030"
     CDS             2345..3967
                     /locus_tag="VFB53_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85540.1"
                     /translation="MALSLRARLLWLVGVALLPVALFALVSLIALGQQQRAQAQQALV
                     ERARAMASAVDQELQGSVAALEVLALSRSLDAGDLRRFHADARAAAGARATWDGILLT
                     DLEGKWLLSTRIPYGAPMPGRGTVVEPGSFAALVAGGRPVVGSIARGPRGRFLFPVRV
                     AVARGGQVRYVLTALVDPADMLGILERQKVPPQSVVTIFDGSQNIVARSRGQSEFAGT
                     PLSATLRALMGDAGEGWGLTRTREGEEVYTAFSRAGPGAWGVALGVEMRALERPISRS
                     YAVSGAGLLLSLAAGGLAAVLLARRISATLAERAALLSEANAGLRASNEQLEAFSYSV
                     SHDLRAPLRAVDANASLVLEAHGAALPAEAQERLRKVSASAQYLARLIDALLAFSQLA
                     RRPVERQRVEPAALVEECVRVLRGERTAQVLVGELPACAADPQLLRQVFHHLIENAFK
                     YSAVVAAPRVEIGWDDGEGAYTVRDNGAGFDMQFAGKLFGLFERLHSPGEYDGTGLGL
                     AIVQRIVQRHGGRVWAEAAPGKGATFYFTLGA"
     gene            complement(3951..5318)
                     /locus_tag="VFB53_02035"
     CDS             complement(3951..5318)
                     /locus_tag="VFB53_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN85541.1"
                     /translation="MDNALRIVPPGIVPPAKPLGRMAFIASFVRNPLTVVPQAAYEDD
                     LVPFARGRAPIMWVTSPALIRTILLDERDKFQKLSQIRLLSPLLGKGILTSEGADWKW
                     QRQASAPMFRPQELAGFVPSFVRAAEEVLARWRARPGEQAVDRDMTEATFQVISATLL
                     PSADARMPQAIERSTGLFQRSGAWGQLYAVANLPKWLPQPGRLSMARAVAALRSAVLS
                     LIGERRAGGAPKDDLLHRLMQARDPETGQSMNDEQLVDNLLTFYLAGHETTAKALTWT
                     LYLVARSPAWARALKDEVERVTGGAELRAEHVAQLSLTQQVLKESMRLFPPVPLMTRQ
                     AVADTMLGGHAVRAGSSVVMPIYAIHRHARRWEDPDAFEPGRFAPEREAAIPRFQYMP
                     FGAGPRICIGMGFAMLEAAAMLATFVRHAEFAPVPGREPAPVARVTLIPGGGMPLEVR
                     LRPRG"
     gene            complement(5549..>5954)
                     /locus_tag="VFB53_02040"
     CDS             complement(5549..>5954)
                     /locus_tag="VFB53_02040"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZN85542.1"
                     /translation="PRVLDEAPRGTRLILHFAGGEFLGPRLKGQVLPGGGDWVLLRRD
                     GVAELDIRLTLRTDDGAMIYARCDGIFHSTPEIRERIRNGEDVDPAEYYFRTAPRFET
                     GVQNYLWLNRLLAVGMGRRTATGMVTEIFAIR"
BASE COUNT          822 a         2102 c         2122 g          908 t
ORIGIN      
        1 tgccgatatc gtgcaggggc gcggccttca tcatcatctc ggccgcctcg ggcggcgcgc
       61 cggccgcctg cgagatcagc ttggcgtagt ggccgaggcg catgacgcgg ttgccgaccg
      121 cggcgctctc gtggaactcc atggcgcgcg caaggcgccg gatgagctgc gagcgcgcgt
      181 cgtccagctg cgcggtgcgc tccgcgacct ggcgctcgag ctcggcctgg cggttggcga
      241 gcgcgaggtg gatgcgcacg cgcgccatca gcaggcgccc gttgatcggc ttggtgaggt
      301 agtccaccgc cccgaggtcc aggccctcca cctggtcgcc ggcctcggtc ttgccggtga
      361 ggaagatcac cgggatgtgc gcggttgccg ggtcttcctt gagcagctgg cagatctcga
      421 agcccgaggc gcccggcatg tccacgtcga gcaggatcag gtcgggcctg gggtcggcgg
      481 cggccagggt cagggcgtcg ccgccgtcgg cggccagctt cacctcgtac tcggtgctca
      541 gcgccttgcc gatgagggcg agcaggtcct cggcgtcgtc caccgcgagg atcaccggcc
      601 gggcattctc cttgctcacc ggggcagtct accgccgagt tcgcaggggc gcttgacaag
      661 ggaaacggtc gtgtataatc gccgccgctg gctccctcaa gcagtcccgg cggtcctcgt
      721 aagatcccgc gttatctact tgaagttccg gcagtgggcg aaagcccgtc acttcaatta
      781 attatttgag aggcaagacg atcatggcaa ctggaacagt caagtggttc aacgatgcga
      841 agggcttcgg cttcatcacc cccgacggcg gcggcaagga cctgttcgcg catttctccg
      901 cgatccaggg ctccggcttc aagagcctga aggacggcca gaaggtctcc ttcgacgtca
      961 ccaccgggcc gaagggcgac caggcgacga acatcaagcc gatgtaactg tcggttcgaa
     1021 gcagcagacg aaaggcccgc gcgagcgggc ctttttcatt ggggtctgac cccatttttc
     1081 gcattttgaa aaatggggtc agaccccttt ttttgcccta tgctccctcg ccatgagtgc
     1141 cttccagggc agaaccgcgt tcgtcaccgg cggcgcctcc ggcatcggcc gcgggctgtg
     1201 cgaggcgctc gccgcgcgcg gcgccgagct caccgtcgcg gacatcgact tcgagggcgc
     1261 gcaggccgtc gccgggggca tcctgcgcgc gggcggcaag gcgcgcgccc tgcgcctcga
     1321 cgtcgcggac ggcgaggccg tgacgcgcgc gctggaatcc gcagggcgcc tggactacgt
     1381 gttcaacaac gcgggcctcg ccatcgtggg cgaggtgcgc gactccgacc tcgcctcctg
     1441 gcggcgcatc gtggacgtca acctgatggg cgtggtggcc gggtcgctcg ccgcctaccg
     1501 gatcatggcg cgccagggca gcggccacat cgtcaacacc gcctcgctcg ccggcctcgc
     1561 cggcatgccg gtgggtgccg cctacgccat gaccaaggcg ggcgtggtga tgttctcgca
     1621 gatgctgcgc gccgagggcg aggcgctcgg cgtcaaggtg agcgccgcct gccccggctt
     1681 catccgcacg ccgatcttcg acaacgcgca gtacctgcgc gtggacaagg cgcgcatgct
     1741 caagttcatc ccgctgccga tcatcgcgct cgcgcccgcc gtcgaaggca tcctcgccgg
     1801 cgtcgggcgc aacgacgcgc tgatcgtgct gccgttctat gcgcgcctga tatggcgcct
     1861 gctgcgctgg ttcccgttcc tcgccggtcc ggtcaaccgc aaggcgatgg ccgatttccg
     1921 gcgcctgcgc attccctgag ggagcgacga tgaccacgtt caggaaagtc acgctgtaca
     1981 cggccggcga cgggctggcg aaattccgcg aggaggacct gcccctgccc gagggcaagc
     2041 cgcaggcgat gctctcggcg ctgttcccct cgtccggcta ccagatgcgc cacagcccgg
     2101 tcggctttcg cagcgactgg cactgctcgc cgctgccgca gtgggtgttc atcctgcagg
     2161 gggagatgga aatccacctg cgcgacggga gttcgcggcg cttccggccc ggcgggcact
     2221 tcttctccac cgacaccgtg cccgcgggcg cggccttcga cccgaagctg cacgggcatt
     2281 ggagccggca ggccgggccg gacgccctgg tgacgctgtt cctcagggcc tgaggggcgc
     2341 gcaggtggct ctgtccctgc gcgccaggct gctctggctg gtcggcgtcg cgctcctgcc
     2401 ggtggcgttg ttcgccctcg tcagcctgat cgcactcggc cagcagcagc gcgcgcaggc
     2461 gcagcaggcg ctggtggagc gcgcgcgcgc catggcgagc gcggtggacc aggagctgca
     2521 gggctcggtc gccgcgctcg aggtgctggc gctgtcgcgc agcctggacg cgggcgacct
     2581 gcggcgcttc cacgccgatg ccagggccgc cgctggcgcg cgcgccacct gggacggcat
     2641 cctgctcacc gacctcgagg gcaagtggct gctgagcacg cgcatcccct acggcgcgcc
     2701 gatgcccggc cgcggaacgg tggtcgagcc gggatccttc gccgccctcg tcgccggcgg
     2761 gcgcccggtg gtcggcagca tcgccagggg gccgcgcggc cgcttcctgt ttccggtgcg
     2821 cgtcgcggtg gcgcgcggcg ggcaggtgcg ctacgtgctc accgcgctgg tggatcccgc
     2881 ggacatgctc ggcatcctcg agcggcagaa ggtgccgccg caatcggtgg tcaccatctt
     2941 cgacggcagc cagaacatcg tcgcgcgctc gcgcgggcag agcgagttcg ccggcacgcc
     3001 gctgtccgct acgctgcgcg cgctgatggg cgacgcggga gagggctggg gcctgacgcg
     3061 cacgcgcgaa ggcgaagagg tctacaccgc cttcagccgc gccgggcccg gcgcctgggg
     3121 cgtggccctg ggggtggaaa tgcgcgcgct cgaacggccg atctcgcgtt cctacgccgt
     3181 gtccggcgcc ggcctgctgc tgtcgctcgc ggccggcggt ctggccgccg tcctgctcgc
     3241 gcggcgcatc tccgccacgc tcgccgagcg cgccgcgctg ctgagcgagg ccaacgccgg
     3301 gctgcgggcc tccaacgagc agctcgaggc cttctcctac tcggtgtcgc acgacctgcg
     3361 cgcgccgctg cgcgcggtgg acgccaacgc cagcctggtg ctcgaggcgc acggcgccgc
     3421 gctgccggcc gaggcgcagg agcgcctgcg caaggtgagc gccagcgcgc agtacctggc
     3481 gcgcctcatc gacgccctgc tcgccttctc gcagctggcg cgccggccgg tggagcgcca
     3541 gcgcgtcgag ccggccgcgc tggtcgagga atgcgtgcgc gtgctgcgcg gcgagcgcac
     3601 ggcgcaggtg ctggtcggcg agctgccggc ctgcgcggcc gacccgcagc tgctgcgcca
     3661 ggtgttccac cacctcatcg agaatgcctt caagtacagc gccgtggtcg ccgcgccgcg
     3721 cgtggaaatc ggctgggacg acggcgaagg cgcctacacc gtgcgcgaca acggcgcggg
     3781 cttcgacatg cagttcgccg gcaagctgtt cggcctgttc gagcgcctgc attcgccggg
     3841 cgagtacgac ggcaccggcc tggggctggc gatcgtgcag cgcatcgtgc agcgccacgg
     3901 cgggcgggtg tgggccgagg ccgcgcccgg caagggcgcg acgttctact tcaccctcgg
     3961 ggcctgagcc gcacctcgag cggcatgccg ccgccgggga tcagcgtgac gcgcgccacc
     4021 ggcgcaggct cgcgtccggg gaccggcgcg aattccgcgt gccgcacgaa ggtggcgagc
     4081 atcgctgctg cctccagcat ggcgaagccc atgccgatgc agatgcgcgg cccggcgccg
     4141 aagggcatgt actggaagcg cgggatcgcc gcctcgcgct ccggcgcgaa gcgcccgggc
     4201 tcgaaggcgt ccgggtcttc ccagcgcctg gcgtggcggt ggatggcata gatcggcatc
     4261 accaccgagc tcccggcgcg cactgcatgg ccgcccagca tggtgtcggc caccgcctgg
     4321 cgcgtcatca gcggcaccgg cggaaagagg cgcatcgact ccttcagcac ctgctgcgtg
     4381 agcgaaagct gcgcgacgtg ctcggcgcgc agctcggcgc cgccggtgac gcgctcgacc
     4441 tcgtccttga gcgcgcgcgc ccaggccggc gagcgcgcga cgaggtacag ggtccaggtg
     4501 agcgccttcg ccgtggtctc gtggccggcg aggtagaagg tgagcaggtt gtccaccagc
     4561 tgctcgtcgt tcatcgactg cccggtctcc ggatcgcgcg cctgcatcag ccggtgcagc
     4621 aggtcgtcct tcggcgcccc gcccgcgcgg cgctcgccga tcagcgacag caccgccgag
     4681 cgcagcgccg ccacggcgcg cgccatcgag aggcggcccg gctgcggcag ccacttcggc
     4741 aggttggcga cggcgtacag ctggccccag gcccccgagc gctgaaacag gcccgtggag
     4801 cgctcgatcg cctgcggcat gcgcgcatcg gccgagggca gcagcgtcgc cgagatcacc
     4861 tggaaggtcg cctcggtcat gtcgcggtcc accgcctgct ccccggggcg ggcgcgccag
     4921 cgcgcgagca cctcctcggc cgcgcgcacg aagctcggca cgaagccggc gagctcctgc
     4981 gggcggaaca tcggcgccga ggcctggcgc tgccatttcc agtccgcgcc ttcgctcgtg
     5041 aggatcccct tgccgagcag cggcgacagc agccggatct gcgagagctt ctggaacttg
     5101 tcgcgctcgt cgagcaggat cgtgcggatc agcgcgggcg aggtcaccca catgatcggc
     5161 gcgcgcccgc gcgcgaacgg caccaggtcg tcctcgtacg cggcttgcgg caccacggta
     5221 agcggattgc gcacgaacga ggcgatgaac gccatgcgac cgagcggctt ggccggcggg
     5281 acgattcccg gaggaacgat tctcagcgcg ttgtccatgc ggccctaaat gaaacgcccc
     5341 gcaccgctcg cgcggccggg gcgtttggtg ctgaggcttg ccggttcagt tgctggcgaa
     5401 gctcctcatc ctgaacgttg atccatagga gcaccgcaca ccgggtaagc cttgggtatc
     5461 tctcactgga tcacctcctt tcgtggttgc cagccgctac tgtagcaaat cagccggcac
     5521 gcgcggaagg tgctcgctgg ccggggcact accggatggc gaagatctcg gtcaccatgc
     5581 cggtggcggt gcgccggccc atccccaccg cgagcagccg gttgagccag agatagttct
     5641 gcacgccggt ttcgaacctg ggcgcagtgc ggaagtagta ttcggcaggg tcgacgtcct
     5701 cgccgttgcg gatccgctcc cggatctcgg gcgtgctatg gaaaatccca tcgcaacggg
     5761 cgtagatcat cgcgccgtcg tcggtgcgca gcgtcagccg gatgtccagt tcggcgaccc
     5821 cgtcgcgtcg cagcagcacc cagtcaccgc cgccaggcag cacctgtcct ttcagcctgg
     5881 ggcccaagaa ctcaccgcca gcgaaatgca ggatgagccg cgtgcctcgc ggtgcttcgt
     5941 cgagcaccct gggc
//
LOCUS       DASRIE010000046         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1612244, whole genome shotgun sequence.
ACCESSION   DASRIE010000046 DASRIE010000000
VERSION     DASRIE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612244"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFB53_02045"
     CDS             <1..329
                     /locus_tag="VFB53_02045"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZN85543.1"
                     /translation="HTSTAAIASLVRLSSFEKRNPLPESNAPVAVIGDARIMLHKEVD
                     PALERERLAKESARLEGEIVKAKAQLAKPSFVERAPAPVVEQHRSRLAGLESTLAKIR
                     QQLEKL"
     gene            complement(326..946)
                     /locus_tag="VFB53_02050"
     CDS             complement(326..946)
                     /locus_tag="VFB53_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85544.1"
                     /translation="MRRRELVLGTAGLLLVRAALAQDRVPQGVSRVRGGATVNGQPAK
                     QGMVVRAGDKVATGADAEIVFVLDRDAMLLRRNGSLEVAKSGFRLVTGAILSVFAPKQ
                     RRELRTPTATIGIRGTAVYLEAEASRTYVCTCYGEAVLEPVDEPAARETVRTRHHEQP
                     RYIMARGAPRMVAPAPVLNHSDAELEMLEALVGRQPPFAGQPGPRY"
     gene            complement(922..1404)
                     /locus_tag="VFB53_02055"
     CDS             complement(922..1404)
                     /locus_tag="VFB53_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85545.1"
                     /translation="MKARWIRIGAIGQQEFDVAHAAIARAQSRIAAPILVWGEEAARY
                     PFALIAPLKFAPGRRERWLPWGLAAAVATYRQFGVPAYLEGDLFLHGRDIAQSRAAAL
                     GECAVIASSFLMRFPGSCVATPSSELEQAFRLRLEAQHGWEFDHSWPTELECAAASSY
                     "
     gene            complement(1401..1889)
                     /locus_tag="VFB53_02060"
     CDS             complement(1401..1889)
                     /locus_tag="VFB53_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN85546.1"
                     /translation="MKRAGVFLALACPFLANAADATFTVRILTPETALKAAQAALKSC
                     RDRGFQTAVAVVDRMGVVQVLLRDRFAGPHTPDMATAKAYTAASFRTGTTELAEASQP
                     GRPASGIRHRPGVAAVGGGLMIEAGGSLLGAIGVSGAPGGREDDACAAAGIAAIREDI
                     EL"
     gene            complement(1886..2113)
                     /locus_tag="VFB53_02065"
     CDS             complement(1886..2113)
                     /locus_tag="VFB53_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZN85547.1"
                     /translation="MNFDKELDARGLNCPLPILRTKKALTDLRPGQVLKVLATDPGAV
                     KDFQTFSRQTGHELLSHAEANREFTFFMRKK"
     gene            2130..3122
                     /locus_tag="VFB53_02070"
     CDS             2130..3122
                     /locus_tag="VFB53_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZN85548.1"
                     /translation="MDEADPRAVPSKKESTAAPVPAWLLTIAQLPVEDPAARMRVMRT
                     LESLGAAVMREGVYLLPDTPGNRQSLDALTDHIAKIHGSAHVLQVNALALAQQEGFAR
                     LFDRSARYENLIKTVESLRVGFGHSDPSAISRVVHKQRREFEAIAALDFFPSGARERA
                     QEALAAADAEVRKLLFPSQSAAALAPGEALLGRTWVTRKPLWADRLACAWLIRRFVDP
                     EATLTWLEKTQPLPADAIGFAFEGAHFANSETRVSYEEMLAKLDLAKNTSLAKIGSIV
                     RFLEVRGEPVAEAAGVQTLLQGAQRRSTSEAELIAEAEKTFDLLYEAYYEPGKR"
     gene            complement(3141..>3483)
                     /locus_tag="VFB53_02075"
     CDS             complement(3141..>3483)
                     /locus_tag="VFB53_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HZN85549.1"
                     /translation="SRPLLYAHIDALQESGRNQDALALLTDSLRLYGRDTRLYAMQSK
                     NYAALGKRSLQHQSQAEVYALQGSLPAAIEQLQLARSAGDGDFYQLSVVDARLKELRA
                     QHAAELRDAKR"
BASE COUNT          534 a         1229 c         1223 g          497 t
ORIGIN      
        1 tgcatacctc gacagcagcc atcgcctcgc tggtgcgtct gagcagcttc gagaagcgca
       61 acccgctgcc cgaatccaac gcgccggtag cggtcatcgg cgatgcccgc atcatgctgc
      121 acaaggaagt cgatcccgcc ctcgagcgcg agcgcctggc caaggaatcg gcgcgcctcg
      181 agggcgagat cgtgaaagcg aaggcgcagc tcgccaaacc ctcgttcgtc gagcgtgctc
      241 cggcaccggt cgtggagcag caccgctccc gcctggccgg cctcgaatcc acgctcgcca
      301 agattcggca gcagctcgaa aagctctagt agcgcggacc cggctggccg gcaaacggcg
      361 gctggcgtcc gaccagcgct tcgagcatct cgagctcggc atcgctgtgg ttgagcaccg
      421 gcgccggcgc caccatccgc ggcgcgccgc gcgccatgat gtaacgcggc tgctcgtggt
      481 gccgggtgcg cacggtctcg cgcgcggcgg gttcgtcgac cggctcgagc accgcctcgc
      541 cgtagcaggt gcagacgtag gtgcggctcg cctcggcctc gaggtagacc gcggtgccgc
      601 ggatgccgat ggtcgcggtc ggcgtgcgca gctcgcggcg ctgcttcggc gcgaacaccg
      661 acaggatcgc gccggtgacg aggcgaaagc cgctcttcgc cacctcgagg ctgccgttcc
      721 gtcgcagtag catcgcgtcg cggtccagca cgaagacgat ctccgcgtcc gcgccggtgg
      781 cgaccttgtc gccggcgcgc accaccatgc cctgcttcgc cggctggccg ttgacggtgg
      841 ccccgccgcg cacgcggctc acgccctgcg ggacgcgatc ctgcgcgagc gccgcgcgga
      901 ccagaagcag cccggccgtg cctagtacga gctcgcggcg gcgcattcga gctcggtcgg
      961 ccaggagtgg tcgaattccc agccgtgctg cgcctccagg cgcaggcgga acgcctgctc
     1021 gagctcgctc gacggcgtcg ccacgcagct gcccgggaag cgcatcagga agctcgacgc
     1081 gatgaccgcg cactccccca gcgcagccgc ccggctctgc gcgatatcac ggccgtggag
     1141 gaagaggtcg ccctccaggt acgccggcac gccgaactgc cggtaggtgg cgacggcggc
     1201 ggcaagaccc caggggagcc agcgctcgcg gcgcccgggc gcgaacttga gcggcgcgat
     1261 cagcgcgaac ggatagcgcg ctgcttcctc tccccaaacc agaatcggcg ctgcgatgcg
     1321 cgactgggcg cgggcgatcg ccgcgtgcgc gacatcgaac tcctgctgcc cgatcgcgcc
     1381 gatcctgatc cagcgagcct tcatagctcg atatcttcgc ggatcgcggc gattcccgcc
     1441 gcggcgcagg catcgtcctc gcgcccgccg ggcgcgccgg agacgccgat cgcgccgagc
     1501 agcgagccgc ccgcctcgat catcagcccg ccgccgaccg cggcgacccc ggggcgatgg
     1561 cggatgccgc tcgccggacg cccgggctgc gaggcctcgg caagctcggt ggtaccggtg
     1621 cgaaagctgg ccgcggtgta cgccttggcg gtcgccatgt cgggcgtgtg cggcccggcg
     1681 aagcggtcgc gcagcagcac ctgcaccacg cccatgcgat cgaccaccgc caccgcggtc
     1741 tggaagccgc ggtcgcggca gctcttcagc gccgcctgcg cggccttcaa cgccgtctcg
     1801 ggggtaagga tcctgacggt aaaggtggca tccgcggcat ttgccagaaa aggacaggca
     1861 agcgccagga acacgcccgc gcgcttcact tcttcctcat gaagaaggtg aactcccggt
     1921 tcgcctcggc gtgcgaaagc agctcatggc cggtctggcg cgagaaggtc tggaagtcct
     1981 tcacggcgcc ggggtcggtg gcgagcacct tcagcacctg ccccgggcgc agatcggtca
     2041 gcgccttctt ggtgcgcagg atcggcagcg ggcagttgag tccgcgcgcg tcgagttcct
     2101 tgtcgaagtt catgaagtta ttatgccgca tggatgaagc agacccgcgc gccgttcctt
     2161 caaagaagga gtcgaccgcc gcgccggtgc ccgcctggct gctgacgatc gcgcagctgc
     2221 cggtggagga cccggctgca cgcatgcgcg tcatgcgcac gctggaatca cttggcgccg
     2281 cggtgatgcg cgagggcgta tacctgctgc cggacacgcc gggcaaccgc cagtcgctcg
     2341 acgcgctcac cgatcacatc gccaagatcc acggcagcgc gcacgtgctg caggtcaacg
     2401 cgctcgcact ggcgcagcag gagggtttcg cacgcctgtt cgaccgctcg gcccgctacg
     2461 agaacctgat caagaccgtc gagagcctgc gcgtgggctt cggccattcg gatccaagcg
     2521 cgatctcgcg cgtggtgcac aagcagcgcc gcgagttcga ggcgatcgcg gccctcgatt
     2581 tttttccgag cggggcgcgg gagcgcgcgc aggaggcgct ggccgcggcc gacgccgagg
     2641 tgcgcaagct tctgttcccg tcgcagtcgg cggcggcgct cgcgccgggg gaagcgctcc
     2701 tcggacgcac ctgggtgacg cgcaagcccc tgtgggccga ccggctggca tgcgcgtggc
     2761 tgatccgccg cttcgtcgat cccgaggcga cgctgacctg gctggagaag acgcagccgc
     2821 tgccggccga cgccatcggc ttcgccttcg agggcgcgca cttcgccaac agcgagacgc
     2881 gcgtcagcta cgaggagatg ctcgccaagc tcgacctcgc caagaacacc tcgctcgcca
     2941 agatcggcag catcgtgcgc ttcctcgagg tgcgcggcga gccggtcgcc gaagcggccg
     3001 gcgtgcagac cctgctgcag ggcgcacagc ggcgctcgac cagcgaggcc gaactcatcg
     3061 ccgaggcgga gaaaaccttc gacctgctgt acgaagccta ctacgagccc gggaagcgct
     3121 agcgaggcaa ggagacgcgc ctagcgcttc gcgtcgcgga gttcggcggc gtgctgagcg
     3181 cgcagctcct tgaggcgcgc gtcgaccacc gagagctggt agaagtcgcc gtcgccggcg
     3241 ctgcgcgcga gctgcagctg ctcgatcgcg gcgggcaggc tgccctgcag cgcgtagacc
     3301 tcggcctgcg actggtgctg cagggagcgc ttgccgagcg cggcgtagtt tttcgactgc
     3361 atcgcgtata agcgcgtgtc gcgcccgtag aggcgcagcg agtccgtgag gagcgccagc
     3421 gcatcctggt tgcggcccga ttcctggagc gcgtcgatgt gcgcgtagag cagcggccgg
     3481 gag
//
LOCUS       DASRIE010000047         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3170412, whole genome shotgun sequence.
ACCESSION   DASRIE010000047 DASRIE010000000
VERSION     DASRIE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170412"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFB53_02080"
     CDS             complement(<1..606)
                     /locus_tag="VFB53_02080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN85550.1"
                     /translation="MDFFDQQDSARRASRWLVLWYLVAVLFVVACFDAAAVLIHLAVT
                     WGQWLPPRAHAVVAGTVAGIILVVTARRMWQLSEGGPAVAAILGAHYLERSRVTPAEA
                     KLLNVVEEMAIASGISVPPVYLLHHEDAINALVAGHTPNEAVIVATRGAVQKLSRDEL
                     QGVMGHEFSHILNGDMALNVRLVGLLAGIACFGEWGEALVYE"
     gene            complement(614..1207)
                     /locus_tag="VFB53_02085"
     CDS             complement(614..1207)
                     /locus_tag="VFB53_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN85551.1"
                     /translation="MGIVGWVILAVVVALAGWAIAVFNRLVALRNRFKNAFAQIDVQL
                     KRRYDLIPNLVEAAKGYLAHERQTLEAVIAARGAAVNAAQQAAAAPGDPAAMVGLAQA
                     EGALGGALGRLMAVFENYPDLKANQNILQVQEELASTENKVAFARQGYNDAVMVYNTK
                     RESFPDNVVAGLTGFTEAMLLEATESAEERKAPKVKF"
     gene            1257..2552
                     /locus_tag="VFB53_02090"
     CDS             1257..2552
                     /locus_tag="VFB53_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85552.1"
                     /translation="MAPFLCRALRAERHELPALAWSFAYFFTLLAGYYVLRPVRDEMG
                     VQAGTGALPWLFSATFVAMLLLVPLYGWLCARLPRARLLPAVYAFFALNLAGFWAALR
                     AGVPVAALAPVFFVWLSVYNLFVVSVFWSYMADLFDTRQAARLFGAIAAGGSAGAIAG
                     PALTALAAAALGTPGLLLVSAALLCASMACIAVLARWSRAHPRAGEPPPEAPLGGSAL
                     AGVRAVLASPFLLGICGYLLCYAMLFTTLYFQQTAIFGAEIPDPRERLRLFATLDLTA
                     NSLALLLQLFVFAPLGALLGVAWMLALMPLLAIAGFAWLGAAPGLGALVTLVVSTRIV
                     EFALSKPVRESLYTAVPREARYKAKSFIDTVVYRGGDAASGWMFGALQGAGLGLAAIA
                     FAMLPLALAWLGLAFFLGSRMRRWMRASDARSAPSPALP"
     gene            2501..3403
                     /locus_tag="VFB53_02095"
     CDS             2501..3403
                     /locus_tag="VFB53_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN85553.1"
                     /translation="MDAGKRRTLGALAGLALMGRAAARESLHARTIPSSGETLPVIGI
                     GTWQSFDVGGDAAARATLAEVLRLFFAGGGRVVDSSPMYGSSEAVAGDACAALGICEP
                     LFMASKVWTSGKAEGIRQMQRSVERMRAGRMDLMQVHNLLDVETHTGTLREWKAAKRV
                     RYIGITHYTASAYAEVERWLKTKQYDFLQINYSIAESESDKRLLPLAKDLGVAVIANR
                     PFAEGAMFRRVRGKPLPPWAAELGIASWAQYFLKWIVSHPAVTCAIPGTGRPEHMRDN
                     LAGGIGKLPDQAQRARMAQAYAAL"
     gene            complement(3400..>4857)
                     /locus_tag="VFB53_02100"
     CDS             complement(3400..>4857)
                     /locus_tag="VFB53_02100"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN85554.1"
                     /translation="SRRVPMNTAFWSATKLLQALRAKKLGALELFDLYAARVREHNRA
                     LNAICVMDLDAGRKSARAADRAKGKRGPLHGLPMTVKESYDVAGWPTTWGLPEHKGNI
                     AQADALAVERLKRAGAIVFGKTNVPVLLADWQSFNPVYGTTNNPWDLLRTPGGSSGGS
                     AAALAAGLTGLEMGSDIGASIRNPAHYCGVYGHKPTWGVCPTRGHALPGVVMPVDITA
                     IGPLGRSADDLALALEVTAGPDAIDAAGWRLALEKPKLASFRKLRVAVLPSHPTAEVD
                     ASVSGALQRLADALAKKGARVSDKARPDFDPDEAHRVYIALLRATTAGRQLPEHVALW
                     EELSQKRAKTDMSYEAQAARGNVMRMKEWPAWSNRRHQMRLLWAEFFKEWDVLLCPTA
                     ATPAFPQNQKGERWERMIDVNGKLQPSTTQMFWAGYSGMCFLPSTIAPVELAGGKLPV
                     GVQIVAPQYADYRSIAVARLIEREYYGFTPPPGYD"
BASE COUNT          624 a         1747 c         1702 g          784 t
ORIGIN      
        1 ctcgtagacc agcgcctcgc cccactcgcc gaagcaggcg atgccggcga gcagccccac
       61 caggcgcacg ttgagcgcca tgtcgccgtt caggatgtgg ctgaactcgt gccccatcac
      121 gccctgcagc tcgtcgcgcg agagcttctg caccgcgccg cgcgtcgcta cgatcaccgc
      181 ttcgttcggc gtgtggccgg cgaccagcgc gttgatcgcg tcctcatggt gcagcaggta
      241 caccggcggc accgagatgc ccgaggcgat cgccatctcc tccaccacgt tgagcagctt
      301 cgcctcggcc ggcgtcacgc ggctgcgctc gaggtagtgc gcaccgagga tcgccgccac
      361 cgccggaccg ccttcggaga gttgccacat gcgccgcgcg gtcaccacca ggatgatgcc
      421 cgccaccgtg ccggcgacga cggcgtgggc gcgcggcggc agccactgtc cccaggtgac
      481 cgccaggtgg atcaggaccg ccgcggcgtc gaagcaggcg acgacgaaca gcaccgccac
      541 gaggtaccac agcaccagcc agcgcgaggc gcggcgcgcg ctgtcctgct ggtcgaaaaa
      601 atccaaggga actctagaac ttcaccttcg gagccttgcg ctcctcggcg gactcggtgg
      661 cttcgagcag catcgcctcc gtgaacccgg tcagcccggc gaccacgttg tcggggaagg
      721 attcgcgctt ggtgttgtag accatcaccg cgtcgttgta gccctggcgc gcgaacgcca
      781 ccttgttctc ggtgctggcg agctcctcct gcacctgcag gatgttctgg ttggccttga
      841 gatcggggta gttctcgaac accgccatca ggcgcccgag cgcgccgccg agcgcgccct
      901 cggcctgcgc cagcccgacc atcgccgccg gatcgcccgg cgccgcagcc gcctgctgcg
      961 ccgcgttcac cgccgcgccg cgcgccgcga tcaccgcctc cagcgtctgg cgctcgtgcg
     1021 cgaggtagcc cttggccgcc tccaccaggt tgggaatcag gtcgtagcgg cgcttcagct
     1081 gcacgtcgat ctgcgcgaag gcgttcttga agcggttgcg cagcgccacc agccggttga
     1141 acaccgcgat cgcccagccg gcaagcgcca ccacgaccgc aaggatgacc cagccaacga
     1201 tacccatcgc gcctcccctt tggcgtgatc cgaattcgcc tcggctacta tagcgcatgg
     1261 ccccgttcct gtgccgcgcg ctgcgcgccg agcggcacga gctcccggcg ctcgcctggt
     1321 cgtttgccta cttcttcacg ctgctcgcgg gctactacgt cctgcggccg gtgcgcgacg
     1381 agatgggcgt gcaggccggc accggcgcgc tgccctggct gttcagcgcg accttcgtgg
     1441 cgatgctgct gctggtgccg ctgtacggct ggctgtgcgc gcgcctgccg cgcgcgcggc
     1501 tgctgcccgc ggtgtacgcg ttcttcgcgc tcaacctcgc cggcttctgg gccgcgctgc
     1561 gcgcgggcgt gccggtcgcc gccctcgccc cggtgttctt cgtctggctg agcgtctaca
     1621 acctgttcgt ggtgtcggtg ttctggagct acatggccga cctgttcgac acgcggcagg
     1681 cggcgcgcct gttcggcgcg atcgccgccg gcggcagcgc cggcgccatc gccggccccg
     1741 cgctcaccgc gctcgccgcg gcggcgctcg gcacgccggg gctgctgctg gtgtcggcgg
     1801 cgctgctgtg cgcgtccatg gcctgcatcg ccgtgctggc gcgctggtcg cgcgcgcatc
     1861 cgcgcgccgg ggaaccgccg ccggaggcgc cgctgggggg atcggcgctc gccggggtgc
     1921 gcgccgtgct tgcctcgccg ttcctgctcg gcatctgcgg ctacctgctg tgctacgcca
     1981 tgctcttcac cacgctgtat ttccagcaga ccgcgatttt cggcgccgag atcccggacc
     2041 cgcgcgagcg cctgcggctg ttcgcgacgc tcgacctgac cgccaattcc ctcgcgctgc
     2101 tgctgcagct gttcgtcttc gcgccgctgg gcgccctgct cggcgtcgcc tggatgctgg
     2161 cgctgatgcc gctcctggcg atcgccggct tcgcctggct gggcgccgcg ccggggctcg
     2221 gcgcgctcgt gacgctggtc gtttccaccc gcatcgtcga gttcgccctg tccaagccgg
     2281 tgcgcgaatc gctgtacacg gcggtgccgc gcgaggcgcg ctacaaggcc aagagcttca
     2341 tcgacacggt ggtgtaccgc ggcggcgacg cggcctcggg ctggatgttc ggcgcgctgc
     2401 agggcgcggg cctcgggctc gccgcgatcg ccttcgccat gctcccgctc gcgctcgcct
     2461 ggctgggact ggcgtttttc ctcgggtcta gaatgcggcg atggatgcgg gcaagcgacg
     2521 cacgctcggc gccctcgccg gccttgcctt gatgggccgg gccgccgcca gggagtccct
     2581 gcacgcgcgg accatcccgt cgagcggcga gacgctgccg gtgatcggca tcggcacctg
     2641 gcagagcttc gacgtgggcg gcgacgccgc ggcgcgcgcg acgctggcgg aagtgctgcg
     2701 cctgttcttc gccggcggcg ggcgcgtggt ggattcttcg ccgatgtacg gctcgagcga
     2761 ggcggtggcg ggcgatgcct gcgcggcgct gggcatctgc gagccgctgt tcatggcgtc
     2821 caaggtatgg accagcggca aggcggaagg catccgccag atgcagcgct ccgtcgagcg
     2881 catgcgcgcc ggacgcatgg acctgatgca ggtgcacaac ctgctcgacg tggagacgca
     2941 caccggcacc ttgcgcgaat ggaaggcggc gaagcgcgtg cgctacatcg gcatcacgca
     3001 ctacaccgcg tcggcctatg ccgaggtgga gcgctggctg aagaccaagc agtacgattt
     3061 cctgcagatc aactactcga tcgcggagag cgaatccgat aagaggctgt tgccgctcgc
     3121 gaaggatctg ggggtggcgg tgatcgccaa ccgcccattc gccgaagggg cgatgttccg
     3181 ccgcgtgcgc ggcaagccgc tgccgccctg ggctgcggag ctcggcatcg ccagctgggc
     3241 gcagtacttc ctcaagtgga tcgtgtcgca cccggcggtg acctgcgcca ttccaggcac
     3301 cggcaggccc gagcacatgc gggacaacct tgccgggggg atcggcaagc tgccggatca
     3361 agcgcagcgc gcgcgcatgg cgcaggccta cgccgcgctc tagtcgtatc ccggcggcgg
     3421 cgtgaagccg tagtactccc gctcgatgag ccgcgccacc gcgatgctgc ggtagtcggc
     3481 gtactgcggc gccacgatct gcacgcccac cggcagcttg ccgccggcca gctcgaccgg
     3541 cgcgatggtg gacggcaaga agcacatgcc cgagtagccg gcccagaaca tctgcgtggt
     3601 ggagggctgc agcttgccgt tcacgtcgat catgcgctcc cagcgctcgc ctttctggtt
     3661 ctgcgggaaa gcgggcgtcg ccgcggtcgg gcaaagcagc acgtcccact ccttgaagaa
     3721 ctcggcccac aggaggcgca tctggtggcg gcggttggac caggcgggcc actccttcat
     3781 gcgcatgacg ttgccgcggg cggcctgcgc ctcgtagctc atgtccgtct tcgcgcgctt
     3841 ctgcgacagc tcctcccaca gcgcgacgtg ctccggcagc tgccggccgg cggtggtggc
     3901 gcgcagcagc gcgatgtaga cgcggtgcgc ctcgtccggg tcgaaatccg gccgcgcctt
     3961 gtcggagacc ctggcgccct tcttcgccag ggcgtcggcg aggcgctgga gggcgcccga
     4021 cacgctcgcg tccacttccg cggtcgggtg cgagggcagc accgccacgc gcagcttgcg
     4081 gaacgacgcc agtttcggtt tctccagcgc gagccgccag cccgcggcat cgatcgcgtc
     4141 cggcccggcg gtgacctcga gcgccagggc gaggtcgtcg gcgctcctgc cgagcggacc
     4201 gatggcggtg atgtcgacgg gcatcaccac ccccggcagc gcgtgccccc gggtcgggca
     4261 aacgccccag gtcggcttgt ggccgtagac gccgcagtag tgcgccggat tgcggatcga
     4321 ggcgccgatg tcgctgccca tctccaggcc ggtgaggccc gcggcgagcg ccgccgccga
     4381 gccgcccgag gagccgcccg gcgtgcgcag caagtcccag ggattgttgg tggtgccgta
     4441 gaccggattg aagctctgcc agtcggcgag cagcaccggc acgttggtct tgccgaacac
     4501 gatggcgccg gcgcgcttga ggcgctccac cgccagcgcg tccgcctgcg cgatgttgcc
     4561 cttgtgctcc ggcagccccc aggtggtcgg ccagccggcc acgtcgtagg actccttcac
     4621 cgtcatcggc aggccgtgca gcgggccgcg cttgcccttc gcgcggtcgg cggcgcgcgc
     4681 cgatttgcgg ccggcgtcga ggtccatcac gcagatcgcg ttcagcgcgc ggttgtgctc
     4741 gcgcacgcgt gcggcgtaga ggtcgaagag ctcgagggcg ccgagttttt tcgcgcgcag
     4801 cgcctgcagg agcttggtgg cggaccagaa tgcggtattc atcgggaccc tccggct
//
LOCUS       DASRIE010000048        11110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2390692, whole genome shotgun sequence.
ACCESSION   DASRIE010000048 DASRIE010000000
VERSION     DASRIE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390692"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFB53_02105"
     CDS             <1..756
                     /locus_tag="VFB53_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN85555.1"
                     /translation="RAQRGFTLTELAVVFAIVALLIGGAMMTLSAQVEQRSNDETVRR
                     LNTAAEAVLAYAIVNRRLPCPATANTTGDEALASGTSATGGPCTTNFGGFLPARTLGF
                     QPVDSAGYGIDVWGNRIRYAVAAGITGCTGSSSTPHFTSLANLKANGVSCRPNDLDIC
                     APVDITTTCTIANRVVSTETVAFIVLSTGKNGAVTTAYGTHESANTDGNATFISRPPT
                     SSDAVTIGTFDDLMVWVPAGVLYAKLISAGVLP"
     gene            complement(720..2462)
                     /locus_tag="VFB53_02110"
     CDS             complement(720..2462)
                     /locus_tag="VFB53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85556.1"
                     /translation="MQTVTASPTLLTRLANGAWSLSQHYWRVVVVAMLALLHVAVFRG
                     VADPWARALLLAHLGLLLLWQPFLRAEQRVSLIQGVILALVASAVMLHLDWWFLAFWV
                     VVLAGLVGGKVYQQHARWQRRCYLVVLAYLLALLAVAILPEIAPHREIDAETRAYAEY
                     LLPLAFALIALFPAEPEPADTPHIIDFFYSVFLMLLLVVVILGSFTFMSVRRTPYLEA
                     LTTTIFLTAGAVLLIALAWNPRSGGGLGVFFARYLFSIGLPVEKWLHFLAELSQVEAR
                     PERFLAEAAAALARLPSVAGLRWRAGEAGGEQGSPTPHTVEYASRELALTIFSRYRLA
                     PALHWHLHLLGQLLGEFYLAKLREEKLHQASYVQAVHETGARMTHDIKNLLQSLSVLC
                     SVATRDDGNSAEAQALLRRQLPLMERRLAETLAKFQRPLAGEIYVAANLWWDALMRQY
                     RGEGVEFEPAVVPTTAQLPRSLFDSVADNLIRNALAKRTADSAVRVTVRLDGGDRVAL
                     RVRDSGAAIPAELSAQLLRAPMNSQSGLGIGLYQAARQAETLGYRLELETNRDGEVCF
                     ALAQGSTPADISLA"
     gene            2503..3264
                     /locus_tag="VFB53_02115"
     CDS             2503..3264
                     /locus_tag="VFB53_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85557.1"
                     /translation="MPSATLARSILLEARRGGLPWLAGASLVVALALAVFLSQVALTE
                     RVALQVALVAAVLRACAVFLIAAQAASSTLREIHDKGLELMLSLPLSRATHYLGRLAG
                     FVVCGAGIALLFSLALLFWAAPPRVALWGVSLACEVALVAAAALFFAMTLANLVAAMT
                     ATAGLYLLARAMPAIQAITASPLADESWAGWLARQTVDAIALLLPRLDTATRTEWLLY
                     DAPSILGYASALAGLLIYALLLAAAGLADFYRRNV"
     gene            3273..3959
                     /locus_tag="VFB53_02120"
     CDS             3273..3959
                     /locus_tag="VFB53_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85558.1"
                     /translation="MRPAAAVPGWLWLLLGTGLAAQLGWQAALGPVRSPDDALPPPPS
                     AQALRLAAFGESEGGARLGMLYLQPFSLRDLDYTRLQGWLGALLELDPRSQYPLFAAA
                     RIYAEHPDPARSRAMLEFVYRQFLLDPNRRWPWLAHGALLAKHRLKDLPLARRYAAAI
                     DRHTTASDVPLWARQMEIFILEDMNELDAARVMLGGLLASGRIQDPAEARFLRERLEE
                     LEVHLRGKTR"
     gene            4098..4733
                     /locus_tag="VFB53_02125"
     CDS             4098..4733
                     /locus_tag="VFB53_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN85559.1"
                     /translation="MKAKGFTLVEIAIVLVIIGLLLGGILKGQEMITQAKIKNIIADF
                     SGVSAAYHGYQDRYRAIPGDDPNAATRWTTPTVAKAGNGNGVVAGKYNSSTADEESRT
                     WWDHLRRAGFVSGSGQQQPFNAVTGMLGVQTGNNAGSTAMGGISGLMLCSANLPDKIA
                     IAVDTQMDDGTNNAGGVRGKLQSQPNPDLDDAPGSGYVETGTNTYVLCRSM"
     gene            4783..6336
                     /locus_tag="VFB53_02130"
     CDS             4783..6336
                     /locus_tag="VFB53_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HZN85560.1"
                     /translation="MRISLAFAALLGALPFLFAEHRAPLPSFYDEWLGAALGTLAVTA
                     FLLGKPSGESALPDLTVWLVLFAAWLGLQALLRPPAYAQLPLAGIAYVLLAAQLAWLG
                     RALADRHGAERVADVLAWAILAGAGVNAAIGIVQFYGVPRVLGEFIATSAGSRVVGHV
                     GQANLLADYVALGQASLLYLVARERIRGGWWWGVAALLVLASACAQSRSAILFSLWIG
                     AAAFCLRDQGPQWRRLARQAGVLAAATLAAMVAIPRLHGTAERLLDAQLILAEPRTAL
                     WALAWRLFAESPWLGVGWGEFAGAAFSAGLPHAGVWTSPHNLFLQLAAEAGLIGAALI
                     LVAALRWSRGAWRDLRSAATLPLWWVAAVAGTIGLHALLEYPLWYAHVLGLAAFVAGI
                     ASRTRFTVPAAPLRGGLAAMSAVAFALLAWTLVDYQRFDRACVIAGGRTLAAPAEVSA
                     AVDELRAVARGPLGAQVAPWLYQSLPPGEVSVDMGERVLRRWPSPLVVARHAAALASA
                     RRGVPPPSR"
     gene            complement(6299..8263)
                     /locus_tag="VFB53_02135"
     CDS             complement(6299..8263)
                     /locus_tag="VFB53_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HZN85561.1"
                     /translation="MDHSAITQSALLPVLVLLASSVLAVAVCRSLRLPPMIGYLVAGL
                     ALGPHALGVVSDRAETHRLAELGVVFLMFSIGLEFSLAKLKSMRRLVFGLGLTQVGAT
                     IGLAVVTALALGTSWQAGLAVGGIVAMSSTAIVSKLLAERGELDSAHGRQVISVLLFQ
                     DLAVVPLLVLIPVLGQPADAIGAAVAAALAKAAAALAIVILAGPRPVRAWLRVVARRR
                     STELFVLNVLLITLAAGFLTAAAGLSLVLGAFLAGMLISETEYRFRVEQDIRPFRDVL
                     LGLFFITVGMMLDLRLVIAQFWLVGLFLLLLIVVKGALIAALSRAFGASGGTPLRVGL
                     ALAQGGEFGFVLLPLASIAGIVPQELLQPMLAAMIVSMFATPFVIAATDRIVLQLSHS
                     EWMLRSLEVHRIAVQSLEAEGHVIVVGYGRNGQRLARLLDAEGVRYVALDLDVERVRE
                     AAAAGDTVVFADGRRREALVAAGIARAAAVVLTFADAATAVRMLAHIHDLKPSLPVIA
                     RARDEADIEPLTAAGASEVVPEAFESGVMLASHALVWVGVPLSRVMRRVRQVRGARYG
                     LLRGLFVGRDEIDSSTRLHSVTLEPGAAATGRTLAELDLGVQVQAVRRRGVKAKLGAA
                     AAGALQAGDVVVLLGSSEAIATAAERLAER"
     gene            8281..9306
                     /locus_tag="VFB53_02140"
     CDS             8281..9306
                     /locus_tag="VFB53_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZN85562.1"
                     /translation="MIKAAQKTPPVASAHGDATIRLARQVLEIEAEAVRALIERVDQS
                     FVDAVALISQRKGRVVVSGMGKSGHIARKIASTLASTGTPAYFVHPAEASHGDLGMIE
                     NGDVFIAISYSGGSEELMQILPQVKRRGAKLIALTGNRDSALAREADVHLDAGVAQEA
                     CPHNLAPTASTTAALALGDALAVALLDRRGFSAADFARTHPRGGLPNQAMAQQALLHV
                     SDVMRSGEKIPKVASGTSLRQAVKEMTRGRIGMTAVVDRAGLVKGVFTDGDLRRTIEQ
                     GTQLDSLRVDEVMTARPRTVRAEMMAAEAVHVMEEHKVNQLLVVDERGALLGALNMHD
                     LFRAKVI"
     gene            9369..9854
                     /locus_tag="VFB53_02145"
     CDS             9369..9854
                     /locus_tag="VFB53_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HZN85563.1"
                     /translation="MVFDVDGVLTDGTLLYGPAGEEMKTFHALDGHGMKMLMESGVAC
                     ALLSGRRSAAVARRAQELGIEHVLQGIDDKLPACEELLKKTGLKMQQAGYMGDELVDL
                     PVLTRCGFACAPREAPAAVRGRVHYVAAAAAGRGAVREVCELVLRAQRKFDAILGKYL
                     A"
     gene            9851..10426
                     /gene="lptC"
                     /locus_tag="VFB53_02150"
     CDS             9851..10426
                     /gene="lptC"
                     /locus_tag="VFB53_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZN85564.1"
                     /translation="MRFSAARLFPLGLMLALALLTFYLERTVREDETPSALRRHDPDY
                     VVTNFTTTTYDRNGAVETVMSAATMVHYPDDDTTDLVAPRLLHVKPQQGRFSVRAERG
                     QLSRDGDEIFLYDNVLLVREATAERPEARMTTEFLHVLRDRSLARTDRPVRIVEGTRL
                     LTGRGMEYNSASRELILRGEVQARFDSVEVE"
     gene            10450..10947
                     /gene="lptA"
                     /locus_tag="VFB53_02155"
     CDS             10450..10947
                     /gene="lptA"
                     /locus_tag="VFB53_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZN85565.1"
                     /translation="MLISFSVLAEKADREKPTQIEANRMSADDVRRLNVFEGNVVVTK
                     GTMVLRADRLVVRQDAEGFQFATATGKPARFRQRQDPKPGEKEGIWIDGEALRIEFDD
                     RNQKIELYESARVNRGGDEVAGDYIFVDQRSDFYQVSAGKGPAGQGRVKAVIQPKVPS
                     AEAGK"
     gene            10949..>11110
                     /locus_tag="VFB53_02160"
     CDS             10949..>11110
                     /locus_tag="VFB53_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85566.1"
                     /translation="MKKQSSLRSQRLSKRYKSRTVVQDVSLDVSSGEVVGLLGPNGAG
                     KTTCFYMIVG"
BASE COUNT         1740 a         3948 c         3819 g         1603 t
ORIGIN      
        1 cgcgcgcagc gcggcttcac gctgacggag ctcgccgtgg tgttcgccat cgttgcgctg
       61 ctcatcggcg gcgccatgat gacgctttct gcacaggtcg agcagcgcag caacgacgag
      121 accgtgcggc gcctgaacac cgcggcggaa gcggtgctcg cctacgccat cgtcaaccgg
      181 cgactaccct gccctgcgac ggcgaatacg acaggggatg aagcactggc gagcggcacc
      241 tccgcgaccg gcggtccctg caccaccaac ttcggcggct tcctgccagc gcgcacgctc
      301 ggcttccagc ccgtcgactc tgccgggtat ggcatcgacg tatggggcaa ccgcatccgc
      361 tatgcggtcg ccgctgggat caccggttgc accggctcgt cgtcgacgcc gcacttcact
      421 tcgcttgcca atctgaaggc caatggcgtg agttgccgcc ccaacgatct cgacatctgc
      481 gccccggtcg acataaccac cacctgcact attgcgaacc gcgtggtcag cacggagacc
      541 gtggcgttca tcgtcctttc gacggggaag aacggtgccg tgacgaccgc ctatggcacg
      601 cacgagagcg ccaacaccga cggcaacgcc actttcatca gccggccccc gaccagctcg
      661 gacgctgtga cgatcggcac gttcgacgac ctgatggtct gggtgccggc cggagtcctc
      721 tacgccaagc tgatatctgc cggcgtgctg ccctgagcta gggcaaagca gacttccccg
      781 tcgcggttgg tctcgagctc gaggcgatag ccgagcgttt ccgcctgccg cgccgcctga
      841 tacaggccga tgccgaggcc gctttgcgag ttcatcggcg cgcgcagcag ctgcgcgctg
      901 agctccgcgg gaatcgccgc gccactgtca cggacgcgca gcgcgacgcg atcgccgccg
      961 tcgagccgca ccgtgacgcg caccgcgctg tcggcggtgc gcttggcgag cgcgttgcgg
     1021 atcaggttgt cggcgacgct gtcgaacagc gagcgtggca gctgcgccgt tgtgggcact
     1081 accgcaggct cgaattcgac gccctcaccg cggtactggc gcatgagggc gtcccaccac
     1141 aggttcgcag cgacgtagat ttcgcccgcc agggggcgct ggaacttcgc cagggtctcg
     1201 gcgaggcgcc gctccatcag cggcagctgc cggcgcagga gcgcttgcgc ctcggccgag
     1261 ttgccgtcgt cgcgggtcgc gaccgagcac agcaccgaga gcgactgcag caggttcttg
     1321 atgtcgtgcg tcatgcgcgc cccggtctcg tgcactgcct gcacgtagct cgcctggtgc
     1381 agcttctcct cgcgcagctt ggcgaggtag aactcgccga gcagctgccc gagaaggtgc
     1441 aggtgccaat gcagcgccgg cgcgagccgg tagcgcgaga agatggtgag ggcgagctcg
     1501 cgcgatgcgt attccaccgt gtgcggcgtg ggactgccct gttccccgcc ggcctcgccg
     1561 gcgcgccagc gcagcccggc caccgaaggc aggcgcgcca gcgccgccgc cgcctcggcg
     1621 aggaagcgct cgggacgcgc ctcgacctgc gacagctcgg cgaggaagtg cagccacttc
     1681 tccaccggca ggccgatgga gaacagatac cgggcgaaga acaccccgag cccgccgccg
     1741 ctgcgcgggt tccacgcgag ggcgatcagc agcacggcgc ccgcggtgag aaaaatcgta
     1801 gtggtgagcg cttccaggta gggcgtgcgc cgtacgctca tgaaggtgaa gctgccgagg
     1861 atcaccacca ccagcagcag catcaggaac acgctgtaga agaagtcgat gatgtgcggc
     1921 gtgtccgcgg gctcgggctc ggcgggaaac agcgcgatca gagcgaacgc gaggggcagg
     1981 aggtattcgg cgtacgcgcg ggtctcggca tcgatctcgc ggtgcggcgc gatctcggga
     2041 aggatcgcca ccgccagcag cgccagcagg tatgcgagca ccacgagata gcagcgccgc
     2101 tgccagcgcg cgtgctgctg atagaccttg ccgccgacca gtccggcgag caccaccacc
     2161 cagaaggcga ggaaccacca gtcgaggtgc agcatcactg ccgaggcgac cagcgccagg
     2221 atcacgccct ggatcagcga gacgcgctgc tcggcgcgca ggaacggctg ccacagcagg
     2281 agcaggccga ggtgcgcgag cagcaaagcg cgcgcccaag ggtcggcgac gccgcggaac
     2341 accgccacgt gcagcagcgc gagcatcgcc accaccacca cgcgccagta gtgctggctc
     2401 aggctccacg caccgttggc gaggcgcgtg agcagcgtcg gcgatgcggt caccgtttgc
     2461 atgaagaatt ccccgcggct agtctagtcc acaacgacaa ccatgccctc ggccacgctc
     2521 gcccgctcga tcctgctcga agcccgccgc ggcggcctgc cctggctggc cggcgcgagc
     2581 ctcgtcgtcg ccctcgcgct cgccgttttc ctctcccagg tcgcgctcac cgagcgcgtc
     2641 gcgctgcagg tggccttggt cgccgccgtg ctgcgcgcgt gcgccgtgtt cctgatcgcg
     2701 gcgcaggccg cctcgagcac gctgcgcgag atccacgaca aggggctcga gctgatgctg
     2761 tcgctgccgc tgtcgcgcgc cacgcactac ctcggccggc tcgccggctt cgtggtctgc
     2821 ggtgcaggca tcgccctgct gttcagcctc gccctgctct tctgggcagc gccgccgcgc
     2881 gtggccttgt ggggcgtttc gcttgcctgc gaggtcgcgc tggtcgccgc cgccgccctg
     2941 ttcttcgcga tgacgctcgc caacctcgtc gccgcgatga ccgcgaccgc cggcctgtac
     3001 ctcctcgcgc gcgccatgcc ggcgatccag gcgatcaccg cgtcgccgct cgccgacgaa
     3061 tcctgggcgg gatggctggc gcggcagacg gtcgacgcga tcgcccttct cctgccgcgc
     3121 ctcgacaccg cgacgcgcac cgaatggctg ctgtacgacg cgccctcgat cctgggctac
     3181 gcgtcggcgc tcgccggtct gctgatctac gcgctgctcc tcgccgccgc gggcctcgcc
     3241 gacttctacc ggaggaatgt ctgagcgcgg acgtgcggcc ggcggcggcg gtgccgggat
     3301 ggctctggtt gctgctgggc acgggcctcg ccgcgcagct tggctggcag gcggcgctcg
     3361 gcccggtgcg ctcgccggac gatgcgctgc cgccgccgcc ctcggcgcag gcgctgcggc
     3421 ttgccgcctt cggcgagagc gagggcggcg cgcgcctcgg catgctttac ctgcagccct
     3481 tcagcctgcg cgacctcgac tacacgcggc tgcaaggctg gctcggcgcg ctgctcgagc
     3541 tcgatccgcg cagccagtat ccgctgttcg ccgcggcgcg catctacgcc gagcatccgg
     3601 atcccgcgcg cagccgcgcg atgctcgaat tcgtgtaccg gcagttcctt ctcgacccga
     3661 accggcgctg gccctggctc gcgcacggcg cgctcctcgc caagcaccgt ctcaaggatc
     3721 tgccgctcgc gcgccgctac gcagccgcga tcgaccggca caccaccgcg tccgacgtgc
     3781 cgctgtgggc gcggcagatg gagatcttca tcctcgagga catgaacgag ctcgacgcgg
     3841 cgcgcgtcat gctcggcggg ctgctcgcga gcggccggat ccaggacccg gcggaagcgc
     3901 gttttctgcg cgagcggctc gaggagctcg aggtccacct tcggggcaag acccgctgag
     3961 ttttcaacag gactgtcgag gattcgacgc ttttacttcc tgaatgtaat gcaacacatt
     4021 gatttataat taactgttct atggcacgcc acttgcacct agacgtctac tcggatggct
     4081 aaaggaaggc cactaatatg aaagcgaagg gcttcacgct ggttgaaatc gcaatcgtgc
     4141 tggtgatcat cggattgctg ctcggcggca tcctgaaagg gcaggaaatg atcacccagg
     4201 cgaagatcaa gaacatcatc gccgacttct cgggggtctc cgccgcctac cacggctacc
     4261 aggaccgcta ccgcgcgatc ccgggcgatg atcccaacgc agccacccgc tggacgaccc
     4321 cgacggtagc gaaagcgggc aacggcaacg gcgttgtcgc cggcaagtac aacagcagca
     4381 ccgccgacga ggagtcgcgc acctggtggg atcacctgcg ccgcgcgggc ttcgtttcgg
     4441 gcagcggcca gcagcagccg ttcaacgcgg tcaccggcat gctcggcgtg cagaccggca
     4501 acaacgccgg cagcactgcg atgggcggca tctccggcct gatgctgtgc tcggccaacc
     4561 tgcccgacaa gatcgccatc gcggtcgaca cgcagatgga cgacggcacg aacaacgcgg
     4621 gtggagtgcg cggcaagttg cagagccagc ccaatcccga cctcgacgat gcgccgggat
     4681 ccgggtacgt ggaaaccggc accaacacct acgtgctttg ccgctccatg taagcgcgca
     4741 gccgcaagga atcgagccgg cctagcgccg gctttttttt cggtgcgcat ctcgctcgcg
     4801 ttcgccgccc tgctgggggc cctgcctttt cttttcgcgg agcatcgcgc gccgctgccg
     4861 agcttctacg acgaatggct cggcgcagcc ctcggcacgc tcgccgtcac ggcctttctc
     4921 ctcgggaagc cttccggcga atcggcgctg cccgatctca ccgtctggct cgtgctgttc
     4981 gccgcctggc tcggcctgca ggccctgctg cgcccgcccg cctatgcgca gcttcccctc
     5041 gccggcatcg cctacgtgct gctcgcggcg cagctcgcct ggctcgggcg cgcgctcgcc
     5101 gatcgccacg gcgcggagcg cgtggccgat gtcctcgctt gggcgatcct cgccggcgcg
     5161 ggcgtcaatg ccgcgatcgg catcgtgcag ttctacggcg tgccgcgcgt gctcggcgaa
     5221 ttcatcgcca ccagcgccgg gtcgcgggtc gtcggacatg tcggccaggc gaacctgctc
     5281 gccgactacg tcgcgctcgg ccaggcatcg ctgctctatc tcgtggcccg cgagcgcatt
     5341 cgcggcggct ggtggtgggg cgtggcagcg ctgctcgtgc tggccagcgc ctgcgcccag
     5401 tcgcgcagcg cgatcctgtt ttcgctctgg atcggcgcgg cggcattctg cctgcgcgac
     5461 cagggcccgc aatggcggcg gctcgcccgc caggcggggg tgctggccgc ggcgacgttg
     5521 gccgcgatgg tggcaatccc tcgtctccac gggaccgccg agcgccttct ggatgcgcaa
     5581 ctcattcttg ccgagccgcg gaccgcgctc tgggcgctcg cgtggcgcct gttcgcggag
     5641 tcgccgtggc tcggcgtggg atggggagag ttcgccggcg ccgcgttcag cgcgggcctg
     5701 ccgcacgcgg gggtgtggac ctccccgcac aatctcttcc tgcagctcgc cgccgaagcg
     5761 ggcctgatcg gcgccgcgct gatcctggtc gcggcgctgc gctggtcgcg cggcgcctgg
     5821 cgggatctgc gcagcgcggc gacgctgccg ctgtggtggg tcgccgcggt cgcgggcacg
     5881 atcggcctgc acgcgctcct cgaatatccg ctctggtacg cgcatgtcct cggcctcgca
     5941 gcgttcgtcg ccggcatcgc ttcgcgaacc cggttcaccg ttccggccgc gccccttcgc
     6001 ggcggcctcg ccgccatgag cgcagtcgcg ttcgcgctgc tcgcctggac cctcgtcgac
     6061 taccagcgct tcgatcgcgc ctgcgtcatt gccggcgggc gcacgctcgc ggcaccggca
     6121 gaagtctcag ccgcagtgga cgagctgcgc gccgtggcgc gcgggccgct cggagcgcag
     6181 gtcgcaccgt ggctctacca gtcgttgccg cccggggagg tttccgtcga catgggcgag
     6241 cgggtcctgc gccgctggcc aagcccgctg gtcgtcgccc ggcatgccgc agcgctcgct
     6301 agcgctcggc gaggcgttcc gccgccgtcg cgatagcctc ggaggagccg agcaggacca
     6361 cgacgtcgcc cgcctgcagc gcgcccgccg ccgccgcgcc gagctttgcc ttcaccccgc
     6421 ggcgacgcac cgcctgtacc tggaccccga ggtcgagctc ggccagcgtc cggccggtcg
     6481 ccgccgcacc cggctcgagc gtcacggagt gcaggcgggt gctggaatcg atctcgtcgc
     6541 gcccgacgaa caggccgcgc agcaggccgt agcgcgcacc gcgcacctgc ctcacgcggc
     6601 gcatgacgcg gctgagcggg acgccgaccc agaccagcgc gtgcgaggcg agcatcacgc
     6661 ccgactcgaa cgcttcgggc accacctcgg aagccccggc agccgtgagc ggctcgatgt
     6721 cggcctcgtc gcgcgcccgc gcgatcaccg gcagcgacgg cttgagatcg tggatgtgcg
     6781 cgagcatgcg caccgcggtc gccgcatcgg cgaaggtgag aaccacggcg gccgcgcgcg
     6841 cgattcccgc cgccaccagc gcctcgcgcc gccgcccgtc ggcgaacacc accgtgtcgc
     6901 cggcggcggc ggcctcgcgc acacgctcca catcgaggtc gagcgcgaca tagcgcaccc
     6961 cctcggcgtc gagcaggcgc gcgaggcgct gtccgttgcg gccgtagccg accacgatca
     7021 catggccctc ggcctcgagc gactgcaccg cgatgcggtg cacctcgagc gagcgcagca
     7081 tccactccga gtgcgagagc tgcagcacga tgcggtcggt cgccgcgatc acgaacggcg
     7141 tcgcgaacat cgagacgatc atcgccgcga gcatcggctg cagcagctcc tggggcacga
     7201 tccccgcgat gctggcgagc ggcagcagca cgaagccgaa ctcgccgccc tgggcgagcg
     7261 cgagcccgac gcgcagcggc gtgccgcccg acgcgccgaa tgcccgcgac agcgcagcga
     7321 tcagcgcgcc cttgacgacg atcagaagaa gcagaaacag ccccaccagc cagaactgcg
     7381 cgatcaccag gcgcaggtcg agcatcatgc cgacggtgat gaagaacagc cccagcagca
     7441 cgtcgcggaa cggccggatg tcctgctcga cccggaagcg gtactcggtc tcagagatca
     7501 gcatgccggc gaggaacgcg ccgagcacca gcgacagccc ggcggcggcg gtgagaaagc
     7561 ccgccgccag cgtgatcagc agcacgttca gcacgaacag ctcggtcgag cgccggcgcg
     7621 ccaccacgcg caaccacgcg cgcaccggcc gcggcccggc caggatcacg atggcgagtg
     7681 cggcggccgc cttggcgagc gcggcggcca ccgccgcgcc gatcgcgtcg gccggctggc
     7741 cgaggaccgg gatgagcacg agcagcggca ccacggcgag atcctggaac aggagcacgg
     7801 agatcacctg gcggccgtgc gcgctgtcga gctcgccgcg ctcggcgagc agcttcgaca
     7861 cgatcgcggt ggaggacatc gcgacgatgc cgccgacggc gagaccggcc tgccacgacg
     7921 tgccgagcgc gagcgcagtg acgacggcga gaccgatggt cgcgccgacc tgcgtcagac
     7981 ccaggccgaa caccaggcgg cgcatcgact tcagcttggc caggctgaac tcgagcccga
     8041 tcgagaacat caggaacacc accccgagct cggcgaggcg gtgcgtctcc gcgcgatccg
     8101 agaccacgcc cagggcatgc ggcccgagcg ccaggccggc gacgaggtag ccgatcatcg
     8161 gcggcaggcg caggctgcgg cacacggcga ccgcgagcac gctcgatgcg agcagcacga
     8221 gcaccggcag cagggccgat tgggtaattg cgctatggtc catgggctat actcccccgc
     8281 atgataaagg cagcgcagaa aacgccgccg gtcgcgtccg cgcatggcga tgcgacgatc
     8341 agactcgcta ggcaggtcct cgaaatcgaa gccgaagcgg tgcgcgcgct gatcgagcgc
     8401 gtcgaccaga gcttcgtcga cgcggtcgcc ctgatcagcc agcgcaaggg gcgcgtggtg
     8461 gtgagcggca tgggaaaatc cggccacatc gcgcgcaaga tcgcctccac cctcgccagc
     8521 accgggaccc ccgcctattt cgtccatccg gccgaggcga gccatggcga cctcggcatg
     8581 atcgaaaacg gcgacgtgtt catcgccatc tcgtactccg ggggcagcga agagctgatg
     8641 cagatcctgc cgcaggtcaa gcggcgcggc gcgaagctca tcgccctgac cggcaaccgc
     8701 gattcagcgc tcgcgcgcga ggcggacgtg cacctcgacg ccggcgtcgc ccaggaagcc
     8761 tgcccgcaca atctcgcgcc gacggcaagc accacggccg ccctcgctct cggagacgcg
     8821 ctcgccgtcg cattgctcga ccggcgcggg ttctccgctg ccgatttcgc gcgtacgcat
     8881 ccgcgcggcg gcctgccgaa ccaggcgatg gcccagcagg cgctcctgca tgtcagcgat
     8941 gtcatgcgca gcggggaaaa gattccgaag gtggcgagcg gcacctcgct gcggcaggcc
     9001 gtgaaggaga tgacgcgtgg ccgcatcggg atgacggcag tcgtggatcg cgcgggcctg
     9061 gtcaagggtg tgtttaccga cggcgacctg cgccggacca tcgaacaagg gacccagctc
     9121 gacagcctgc gggtggacga ggtcatgacc gcccgcccgc ggacggtgcg cgccgaaatg
     9181 atggccgccg aggcggtgca cgtcatggag gagcacaagg tcaatcagtt gctcgtggtc
     9241 gacgagcgcg gggcgttgct gggcgcgctc aacatgcacg acctgttccg cgccaaggtg
     9301 atttgaaaga ggctgcgcga acgcggctcg gccggcagtc gctcgcgcgg gcgcgcggcg
     9361 tgcggctgat ggtgttcgat gtcgacggcg tgctgaccga cggcaccctg ctgtacggac
     9421 ccgccggaga ggagatgaag acctttcacg cgctggacgg ccacgggatg aagatgctga
     9481 tggaaagcgg ggtggcctgc gccctgctct ccggacggcg ctcggcggcc gtggcgcggc
     9541 gcgcccagga gctcggcatc gagcacgtgc tgcaggggat cgacgacaag cttcccgcct
     9601 gcgaggaatt gctgaagaaa acaggcctca agatgcagca agccggctac atgggcgacg
     9661 agctcgtcga cctgccggtc ctgacgcgct gcggcttcgc ctgcgcgccg cgcgaggcgc
     9721 ccgcggccgt gcgcggccgc gtgcattacg tcgccgccgc cgcggccggc cgcggcgcag
     9781 tgcgcgaggt gtgcgagctg gtgctgcgcg cacagcgcaa gttcgacgcg atcctgggga
     9841 aatacctcgc gtgagattct cggccgcccg actgttcccg ctgggcctga tgctggcgct
     9901 cgcgctgctc accttctatc tcgagcgcac ggtgcgcgag gacgagacgc cgtcggcgct
     9961 gcgccgccat gatccggatt acgtggtgac caacttcacc accaccacct acgaccgcaa
    10021 cggcgccgtc gagacggtga tgtcggcggc gacgatggtg cactacccgg acgacgacac
    10081 caccgatctg gtcgcgccgc gcctgctgca cgtcaagccg cagcaggggc gcttctcggt
    10141 gcgcgccgag cgcggccagc tgtcgcgcga cggcgacgag atcttccttt acgacaacgt
    10201 gctgctggtg cgcgaggcga ccgccgagcg gcccgaggcg cgcatgacca ccgaattcct
    10261 gcacgtgctg cgcgaccgtt cgctggcgcg caccgaccgc ccggtcagga tcgtcgaggg
    10321 cacgcgcctg cttaccggcc gcggcatgga gtacaacagt gcgtcgcgcg agctcatcct
    10381 gcgcggcgag gtccaggcgc gcttcgactc ggtcgaggtc gaatgaagcc ggcatcgctc
    10441 gcgctcctca tgttgatttc tttttccgtg cttgcggaga aagccgatcg cgaaaaaccg
    10501 acccagatcg aggcgaatcg catgagcgcc gacgacgtga ggcggctgaa cgtgttcgag
    10561 ggcaacgtgg tcgtcaccaa gggcaccatg gtgctgcgcg ccgaccgcct ggtggtgcgc
    10621 caggacgcgg aggggttcca gttcgcgacc gccaccggca agccggcgcg cttccgccag
    10681 cgccaggatc ccaagcccgg cgagaaggaa ggcatctgga tcgacggcga ggcgctgcgc
    10741 atcgagttcg acgaccgcaa ccagaagatc gagctgtacg agagcgcgcg cgtcaaccgc
    10801 ggcggcgacg aggtcgccgg cgactacatc ttcgtcgatc agcgctccga cttctatcag
    10861 gtcagcgcgg gcaagggacc cgccggacag ggccgggtca aggcggtgat ccagcccaag
    10921 gtcccgagcg ccgaagcggg aaaatagctt gaaaaagcaa agctcccttc gctcgcagcg
    10981 gctgagcaag cgctacaagt cgcgcaccgt ggtgcaggac gtgtcgctcg acgtgtcgag
    11041 cggcgaggtg gtcgggctgc tcgggccgaa cggcgcgggc aagaccacct gcttctacat
    11101 gatcgtcggg
//
LOCUS       DASRIE010000049         7208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3171069, whole genome shotgun sequence.
ACCESSION   DASRIE010000049 DASRIE010000000
VERSION     DASRIE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3171069"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="rho"
                     /locus_tag="VFB53_02165"
     CDS             <1..375
                     /gene="rho"
                     /locus_tag="VFB53_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN85567.1"
                     /translation="KPKRFFGAARNVEEGGSLTIIATALIDTGSRMDDVIYEEFKGTG
                     NMEIHLDRRMHEKRIFPSINVNRSGTRREELLIAKELLQKIWVLRKLLFPMDELEAAE
                     FLIDKLKATKNNGEFFDSMRRG"
     gene            435..695
                     /locus_tag="VFB53_02170"
     CDS             435..695
                     /locus_tag="VFB53_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HZN85568.1"
                     /translation="MKPTGHPDYQPVIFLDTSSNHSFLSRSAIDTKGREKMKWSDGLE
                     YPVVKVEVSSESHSYYTGKQKIVDTAGRVEKFKRRYTKAAKK"
     gene            724..2313
                     /locus_tag="VFB53_02175"
     CDS             724..2313
                     /locus_tag="VFB53_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85569.1"
                     /translation="MIPRLALPPAPAVLALLALAFVLPGLAGHDPWKAFDALGIEIAH
                     QMHLTGDWLVPRVAGEPWLDDPPLYHWLALAFGKLLGWALPFHAAARLASGLCVLAAL
                     WLLYRTAGAAALLLLVGSVGLMVHAHEALPELAALAASCAAYAVLARAHRRPLAMGAA
                     FGAALGLAFLALGPLVPATLAAAVLAAHAACPAWRTRRAAPFLLAALAAGTLIAASWP
                     ALLAQRSPELLGAWWSLATHTRGGALENLRYFAVTGSWFAWPAWPLAAWALWTRRRAW
                     REPRLFVPAAAFALGFAALAVAGPAQDVSLIALLAPLALLGAQGVAALRRGAAAALDW
                     FGAMTFTFFGALIWLGYLALQTGWPPKIQHNFLKTAPGFESQFAPLAFLFALALLLGW
                     LYVAFYTSPSPMRGVTRWAAGITLLWGSFAMLWLPWADYQKSYRAVALQLKTKVPPIA
                     GCIAAQNLGSPQRAALSYHAGIRTQALDRAQPAACRLLLVQGSPRDERDGPNASWTKL
                     ADVGRPGDKGERYRLYRLEAP"
     gene            2310..3704
                     /locus_tag="VFB53_02180"
     CDS             2310..3704
                     /locus_tag="VFB53_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZN85570.1"
                     /translation="MTLNTTPPTGCPAWAKLAVHAEGWREARLADLFAGDAQRAAQFV
                     ARAPGLALDYSRQRCGALTVRLLAQLAAERGLEGWRDALFAGAPINVTEDRAARHAEL
                     RAGGAAGAEAKALARGMQGVRRIVHLGTGGSELGPRLLYDALREQARIEVRFAANADP
                     LDLARALEGAEPASTLVLAVSKTFTTAETLANAKAAKDWLGGKGRLYAVTANEAAARA
                     FGAQQVLPLQDWVGGRFSVWSAASFSALAALGEGAFDEFLAGGRDIDRHFREAPLERN
                     LPVLLALVGVWNVNFLGAATHCVLPYAHALRLLPAWLQQLEMESNGKRVDRAGNALEY
                     ATAPVLWGAEGTPSQHSFHQLLHQGTQVVPADFIVAGVSAQMEANAEAQSRALAFGTG
                     DAALPPHRRTPGNRPSSILRLDRLDARHLGSLLAAYEHKVFVQGVLWNINSFDQWGVE
                     LGKRLADDILRKGS"
     gene            3708..3914
                     /locus_tag="VFB53_02185"
     CDS             3708..3914
                     /locus_tag="VFB53_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HZN85571.1"
                     /translation="MDQDTFNLSIRKFLKMVGVNSQREIEQAVLQALAAARLKGEETI
                     AARVTLEIPSLGVRVPFEGEIKLK"
     gene            3919..5304
                     /locus_tag="VFB53_02190"
     CDS             3919..5304
                     /locus_tag="VFB53_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN85572.1"
                     /translation="MNKAREFDQSVLTELQALLGERVTVSAGIREHHGKDESYLPYAP
                     PDAVAFPESTEEVRDIVNICRRHHVPMIPYGVGTSLEGHILAIHGGVCIDMSRMNRVL
                     AVHQEDLDAVVQAGVTRKQLNEYVKHTGLFFPIDPGADATLGGMSATRASGTNAVRYG
                     TMRENVLSLKVVLADGRIVQTARRAKKSSAGYDLTRLFVGSEGTLGVITEVTVKLHPV
                     QAAMSAAVCAFASVKGAADTVIQTLQSGIPIARSELLCATTMAALNRHNKTSHREMPT
                     LLLEFHGTEAAVVEQAEMVQEIAKDNGGLGFQWATRPEERSKLWEARHNAYFACLQLK
                     AGSRAISTDVCVPISRLAECIVETTKDIEKASMPIPLFGHVGDGNFHLVILIDPASQS
                     DMDEAKKINLRVVQRALAMEGTCTGEHGIGIGKQTYLAEELGEAVDLMKDLKRAFDPE
                     NLFNPGKVVRL"
     gene            5301..5759
                     /locus_tag="VFB53_02195"
     CDS             5301..5759
                     /locus_tag="VFB53_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN85573.1"
                     /translation="MSAEERLKKLGIELGAVSAPVANYVNAVRTGNLLYLSGKGPRPV
                     NGVRPKGKLGREFTVEQGAAHARSVGLDLLAVMRAELGSLDRVKRVVKLLGMVNAVPE
                     FEDHPRVINGCSDLLVEVLGEAGRHARSAVGMGSLPMGIPVEIEVIVEVG"
     gene            complement(5756..6301)
                     /gene="orn"
                     /locus_tag="VFB53_02200"
     CDS             complement(5756..6301)
                     /gene="orn"
                     /locus_tag="VFB53_02200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZN85574.1"
                     /translation="MPADQNNLIWIDMEMTGLLPDSDRIIEVAVLVTDAQLNLVAEGP
                     VLVLRQPDEVLAAMDSWNRGVHGKSGLIDKVKASTLDEAAVEARLLEFLAPLVPAGAS
                     PMCGNSICQDRRFLARSMPRLEAYFHYRNLDVSTLKELVKRWKPELVKGLKKEGRHEA
                     LADILESIEELKYYRRTAMTI"
     gene            6320..>7208
                     /locus_tag="VFB53_02205"
     CDS             6320..>7208
                     /locus_tag="VFB53_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZN85575.1"
                     /translation="MSNAFTWLFLAALAGATLTRLWLARRQAAHVRAHRDAVPAEFAE
                     VMPLSAHQKAADYTVAKLRLGAADLLVGTAALLALTLGGALRWLAEQWAAVFAPGGLA
                     HGTALVASVVLLLAVIELPASVYRTFAIEARFGFNRMTPALFAADLARHALLGAALGL
                     PLLVLVLWLMERMGPQWWLAVWCAWAGFNLLILFLYPTFIAPLFNKFTPLADPALAVR
                     IDALLARCGFRAKGLYVMDGSKRSAHGNAYFTGFGAAKRIVFFDTLLARLAPAEVEAV
                     LAHELGHFRRHHVWKRIALM"
BASE COUNT         1016 a         2627 c         2504 g         1061 t
ORIGIN      
        1 aagcccaagc gcttcttcgg cgcggcgcgc aacgtcgagg aaggcggctc gctcaccatc
       61 atcgccaccg cgctgatcga caccggcagc cgcatggacg acgtgatcta cgaggaattc
      121 aagggcaccg gcaacatgga gatccacctc gaccggcgca tgcacgagaa gcgcatcttc
      181 ccctcgatca acgtcaaccg ctcgggcacg cgccgcgagg agctgctgat cgcgaaggag
      241 ctgctgcaga agatctgggt gctcaggaaa ctcctcttcc ccatggacga gctggaggcc
      301 gccgaattcc tcatcgacaa gctgaaggcc accaagaaca acggcgagtt cttcgactcg
      361 atgcgccgcg gctaggcttg aattaagtca ttgttttctg tatagttcgc gccttttcca
      421 gagccgccca cgccatgaaa cccacaggcc accccgacta ccagccggtg atcttcctcg
      481 acaccagctc caaccacagc ttcctgtcgc gctccgcgat cgacaccaag ggccgcgaga
      541 agatgaaatg gtccgacggc ctcgagtacc cggtggtcaa ggtcgaggtg tcctcggagt
      601 cccactccta ctacaccggc aagcagaaga tcgtcgacac cgccggccgc gtggagaagt
      661 tcaagcgccg ctacaccaag gccgcgaaga agtaagccgg ccgggcgcgc ctagaatgcg
      721 cgcatgattc cccgcctcgc cctgccgccc gcgcccgcgg tgctggcgct gctggcgctg
      781 gctttcgtgc tgccgggcct cgccgggcac gacccctgga aggcgttcga cgcgctcggc
      841 atcgagatcg cccaccagat gcacctcacg ggcgactggc tggtgccgcg cgtcgccggc
      901 gagccctggc tcgacgaccc gcccctgtac cactggctcg cgctcgcctt cggcaagctg
      961 ctcggctggg cgctgccctt ccatgccgcc gcgcgcctgg cgagcgggct gtgcgtgctc
     1021 gccgcgctgt ggctgctgta ccgcacggcg ggcgcggcgg cgctgctgct cctcgtcggc
     1081 tcggtcggcc tgatggtgca cgcgcacgag gcgctgccgg agctcgcggc gctcgccgcg
     1141 agctgcgcgg cctacgcggt cctggcgcgc gcgcaccggc ggcccctcgc catgggcgcg
     1201 gccttcggcg cggcgctcgg cctcgccttc ctcgcgctgg ggccgctcgt cccggcgacg
     1261 ctcgcggcgg cggtgctcgc cgcgcatgcc gcctgcccgg cctggcgcac gcggcgcgcg
     1321 gcgccgttcc tgctcgcggc gctcgccgcc ggcacgctca tcgccgcgag ctggcccgcg
     1381 ctgctcgcgc agcgctcccc ggagctcctc ggcgcctggt ggtcgctcgc cacgcacacg
     1441 cgcggcggcg cgctggagaa cctgcgctac ttcgccgtca ccggcagctg gttcgcctgg
     1501 cccgcctggc cgctcgccgc ctgggcgctg tggacgcggc gccgtgcctg gcgcgagccg
     1561 cgcctgttcg tgccggcggc cgccttcgcc ctcggcttcg ccgcgctcgc cgtcgccggc
     1621 ccggcgcagg acgtgagcct gatcgccctg ctcgcgccgc tcgccctcct cggcgcgcag
     1681 ggcgtggccg cgctgcgccg cggcgccgcg gcggcgctcg actggttcgg cgccatgacc
     1741 ttcaccttct tcggcgcgct catctggctg ggctacctcg cgctgcagac cgggtggccg
     1801 cccaagatcc agcacaactt cctcaagacc gcgccgggct tcgagtcgca gttcgcgccg
     1861 ctggcgttcc tgttcgcgct ggcgctgctg ctcggctggc tgtacgtcgc cttctacacc
     1921 tcgccctcgc cgatgcgcgg cgtgacgcgc tgggccgccg gcatcacgct cctgtggggc
     1981 agcttcgcca tgctgtggct gccctgggcg gactaccaga agagctaccg cgcggtggcg
     2041 ctgcagctga agaccaaggt gccgccgatc gccggctgca tcgccgcgca gaacctgggc
     2101 agcccgcagc gcgccgcgct cagctaccac gccggcatcc gcacccaggc gctggaccgc
     2161 gcgcagccgg ccgcctgccg gctgctgctg gtgcagggca gcccgcgcga cgagcgcgac
     2221 ggaccgaacg cctcctggac gaagctcgcc gacgtcgggc ggcccggcga caagggcgag
     2281 cgctaccgcc tgtaccggct cgaggcgccg tgaccctgaa cacgacgccg cccaccggct
     2341 gccccgcctg ggccaagctc gccgtgcacg ccgagggctg gcgcgaagcg cgcctggcgg
     2401 acctgttcgc cggcgacgcg cagcgcgccg cgcagttcgt cgcgcgcgcc ccgggcctcg
     2461 cgctcgacta ctcgcgccag cgctgcggcg cgctcaccgt gcgcttgctc gcccagctcg
     2521 ccgccgagcg cggcctcgag ggctggcgcg atgcgctgtt cgcgggcgcg ccgatcaacg
     2581 tcaccgagga tcgcgcggcg cgccatgccg agctgcgcgc gggcggcgcg gcgggcgccg
     2641 aggcgaaggc gctcgcgcga gggatgcagg gcgtgcgccg catcgtgcac ctgggcaccg
     2701 gcggctcgga gctcgggccg cggctcctct acgacgcgct gcgcgagcag gcgaggatcg
     2761 aggtgcgctt cgccgcgaac gccgacccgc tcgacctcgc gcgcgcgctc gaaggcgccg
     2821 agcccgcgag caccctggtg ctcgccgtgt ccaagacctt caccaccgcc gagacgctcg
     2881 ccaacgcgaa ggcggcgaag gactggctcg gcggcaaggg gcggctctac gcggtgaccg
     2941 cgaacgaagc cgccgcgcgc gccttcggcg cgcagcaggt gctgccgctg caggactggg
     3001 tcggcgggcg cttctcggtc tggtcggcgg cgagcttcag cgcgctcgcc gcgctcggcg
     3061 aaggcgcgtt cgacgagttc ctcgccggcg gccgcgacat cgaccggcat ttccgcgaag
     3121 cgccgctcga gcgcaacctc cccgtgctgc tcgcgctcgt tggcgtgtgg aacgtgaatt
     3181 tcctcggcgc ggcgacgcac tgcgtgctgc cctacgcgca tgccctgcgc ctgctgccgg
     3241 cctggctgca gcagctggag atggaatcca acggcaagcg cgtcgaccgc gccggaaacg
     3301 cgctcgagta cgccaccgcg ccggtgctgt ggggcgccga gggcacgccg agccagcatt
     3361 ccttccacca gttgctgcac cagggcacgc aggtcgtgcc ggcggacttc atcgtcgcgg
     3421 gggtgagcgc gcagatggag gccaatgccg aggcgcagtc gcgcgcgctc gccttcggca
     3481 ccggcgacgc cgcgctgccg ccgcaccggc gcacccccgg caaccggccc tcgagcatcc
     3541 tgcggctgga ccggctcgac gcgcgccacc tggggagcct cctggccgcc tacgagcaca
     3601 aggtgttcgt gcagggcgta ctctggaaca tcaacagctt cgaccagtgg ggcgtcgagc
     3661 tgggcaagcg gctggccgac gacattcttc gcaaagggag ttagggcatg gaccaggaca
     3721 ccttcaacct cagcatccgc aagttcctca agatggtggg cgtcaactcg cagcgcgaga
     3781 tcgagcaggc ggtgctgcag gcgctcgccg cggcccgcct caagggcgag gagaccatcg
     3841 ccgcgcgggt caccctggag atcccctcgc tcggggtgcg cgtgccgttc gagggcgaaa
     3901 taaagctaaa atgatccggt gaacaaggcg cgcgaattcg accagagcgt ccttaccgag
     3961 ctgcaggcgc tgctcggcga gcgggtgacg gtgtccgcgg gcatccgcga gcaccacggc
     4021 aaggacgagt cctacctgcc ctacgcgccg cccgacgcgg tggccttccc cgagtccacc
     4081 gaggaagtgc gcgacatcgt caatatctgc cgccgccacc acgtgccgat gatcccctac
     4141 ggcgtgggca cctcgctgga gggccacatc ctcgccatcc acggcggcgt gtgcatcgac
     4201 atgagccgca tgaacagggt gctggcggtg caccaggagg acctcgacgc ggtggtgcag
     4261 gccggcgtga cgcgcaagca attgaacgag tacgtgaagc acacgggcct gtttttcccc
     4321 atcgacccgg gcgccgacgc gaccctcggc ggcatgagcg ccacgcgcgc ctcgggcacc
     4381 aacgccgtgc gctacggcac catgcgcgag aacgtgctgt cgctgaaggt ggtgctggcc
     4441 gacggccgca tcgtccagac cgcgcgccgc gcgaagaaat cctccgcggg ctacgacctg
     4501 acgcgcctgt tcgtcggctc ggagggcacc ctgggcgtga tcaccgaggt gacggtgaag
     4561 ctgcacccgg tgcaggcggc gatgtcggcc gcggtgtgcg ccttcgccag cgtgaagggc
     4621 gcggcggaca ccgtgatcca gacgctgcag tcgggcatcc cgatcgcgcg cagcgaactg
     4681 ctgtgcgcca ccaccatggc ggcgctcaac cggcacaaca agaccagcca ccgcgagatg
     4741 ccgacgctgc tgctcgagtt ccacggcacg gaagccgcgg tggtcgagca ggccgagatg
     4801 gtgcaggaga tcgcgaagga caacggcggc ctcggcttcc agtgggccac ccggcccgag
     4861 gagcgcagca agctctggga agcgcgccac aacgcctact tcgcctgcct gcagctgaag
     4921 gccggctcgc gcgccatctc gaccgacgtc tgcgtgccga tctcgcgcct cgccgagtgc
     4981 atcgtcgaga cgaccaagga catcgaaaag gcctcgatgc cgatcccgct cttcggccac
     5041 gtcggcgacg gcaacttcca cctggtgatc ctcatcgacc cggcgagcca gtccgacatg
     5101 gacgaggcga agaagatcaa cctgcgcgtg gtgcagcgcg ccctcgccat ggaaggcacc
     5161 tgcaccggcg agcacggcat cggcatcggc aagcagacct acctcgccga ggagctgggc
     5221 gaggcggtgg acctgatgaa ggatctcaag cgcgccttcg acccggagaa cctgttcaac
     5281 ccgggcaagg tcgtccggct ttgagcgccg aagagcgcct gaaaaagctg ggcatcgagc
     5341 tcggcgccgt gtcggcgccg gtcgccaact acgtcaacgc ggtgaggacc gggaacctgc
     5401 tctacctctc gggcaagggt ccgcgaccgg tgaacggggt gcgtcccaag ggcaagctcg
     5461 gccgcgagtt caccgtcgag caaggagctg cgcacgcgcg ctcggtgggc ctcgacctcc
     5521 tcgcggtgat gcgcgcggaa ctcggcagcc tggatcgcgt gaagcgcgtg gtgaagctcc
     5581 tcggcatggt caacgccgtg cccgagttcg aggaccatcc cagggtcatc aacggctgct
     5641 cggacctgct ggtggaggtg ctgggcgagg ccggcaggca cgcccgctcg gcggtcggca
     5701 tgggctcgct cccgatgggg atcccggtcg agatcgaagt gatcgtcgag gtcggctaga
     5761 tcgtcatcgc cgtgcggcgg tagtacttca gctcctcgat cgattcgagg atgtcggcga
     5821 gggcttcgtg cctgccctcc ttcttcaggc ccttgaccag ctccggcttc cagcgcttga
     5881 cgagctcctt cagcgtggag acgtcgaggt tgcggtagtg gaagtaggcc tccagccgcg
     5941 gcatcgagcg cgcgaggaag cgccggtcct ggcagatcga gttgccgcac atcggcgagg
     6001 cgccggcggg caccagcggc gcgaggaact ccagcagccg cgcttccacc gccgcctcgt
     6061 cgagcgtgga ggccttgacc ttgtcgatca gcccggactt gccgtgcacg ccgcggttcc
     6121 acgagtccat cgccgccagc acctcgtcgg gctggcgcag caccagcacc gggccctcgg
     6181 cgacgaggtt gagctgcgcg tcggtgacca gcacggccac ctcgatgatg cggtcggagt
     6241 cggggaggag ccccgtcatc tccatgtcga tccagatcag gttgttctgg tccgcaggca
     6301 tgtcgtattc tctcacggcg tgtcgaatgc ctttacctgg ctgttcctcg cggcgctcgc
     6361 cggcgcgacg ctgacgcggc tgtggctcgc ccggcgccag gcggcgcacg tgcgcgcgca
     6421 ccgcgacgcg gtgccggcgg aattcgccga ggtgatgccg ctgtcggcgc accagaaggc
     6481 ggccgactac acggtggcca agctgcgcct gggcgcggcc gacctgctgg tgggcaccgc
     6541 ggcgctgctc gcgctcacgc tgggcggcgc gctgcgctgg ctggcggagc agtgggcggc
     6601 ggtgttcgcg ccgggcggcc tggcgcacgg caccgccctg gtggcgagcg tggtgctgct
     6661 gctcgcggtg atcgagctgc ccgcctcggt ctaccgcacc ttcgccatcg aggcgcgctt
     6721 cggcttcaac cgcatgaccc cggccctgtt cgccgcggac ctggcgcgcc acgcgctgct
     6781 cggcgccgcg ctcggcctgc cgctgctggt gctggtgctg tggctgatgg agcgcatggg
     6841 gccgcagtgg tggctcgccg tctggtgcgc ctgggccggc ttcaacctgc tgatcctgtt
     6901 cctctacccc acgttcatcg cgccgctgtt caacaagttc acgccgctcg ccgaccccgc
     6961 gctcgccgtg cgcatcgacg cgctgctcgc gcgctgcggc ttccgcgcca agggcctgta
     7021 cgtgatggac ggctcgaagc gctcggcgca cggcaacgcc tatttcaccg gcttcggcgc
     7081 cgccaagcgc atcgtgttct tcgacaccct gctcgcgcgc ctggcgccgg cggaggtgga
     7141 ggcggtgctg gcgcacgagc tgggccactt ccggcgccac cacgtgtgga agcgcatcgc
     7201 gctgatgt
//
LOCUS       DASRIE010000050         9278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_576748, whole genome shotgun sequence.
ACCESSION   DASRIE010000050 DASRIE010000000
VERSION     DASRIE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576748"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFB53_02210"
     CDS             <1..550
                     /locus_tag="VFB53_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN85576.1"
                     /translation="SAGAKTRKAVTIKSHHNVGGLPKSLKLKLLEPLRELFKDEVREL
                     GVALGLPREMVFRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEELRAAGWYDTTA
                     QAFAVFLPVKSVGVMGDGRTYEYVVALRAVQTTDFMTAAWAPLPHELLAKVSSRIINE
                     VRGINRVAYDISSKPPATIEWE"
     gene            complement(561..740)
                     /locus_tag="VFB53_02215"
     CDS             complement(561..740)
                     /locus_tag="VFB53_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85577.1"
                     /translation="MKLNRSIATAILLGCVMAGLPACEKKGPVEKAGKAVDDAAKKTG
                     DAIREAGDKIKDAAK"
     gene            complement(815..1645)
                     /locus_tag="VFB53_02220"
     CDS             complement(815..1645)
                     /locus_tag="VFB53_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZN85578.1"
                     /translation="MRLGDQRASANVEDRRGMRLGGGLGIGGIAIAVVAYFLGFDPAA
                     VLNMAGQVAPQSESRVAPKGAPADAGGQFVAKVLGSAEDVWSGIFQQSKSQYRPPTLV
                     LYEGQVRSACGMGQSATGPFYCPADEKLYIDLAFFRDLQTRFGAPGDFAQAYVIAHEV
                     GHHVQQLTGKFQKMQDARASGSRAQANQASVRMELQADCYAGVWGHHAGTLKQLEPGD
                     IAEALNAASAIGDDRLQKQTQGQVVPESFTHGSSAQRVHWFKRGMASGRPQDCDTLDD
                     "
     gene            1746..2171
                     /gene="tadA"
                     /locus_tag="VFB53_02225"
     CDS             1746..2171
                     /gene="tadA"
                     /locus_tag="VFB53_02225"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZN85579.1"
                     /translation="MTDEDFMRLALELAARTEAEVPVGALVVLGGKAVGEGWNRPLAT
                     HDPTSHAEIEAIRAAGKATGNYRLPGATLYVTLEPCTMCVGAIFNARIARVVYGATEP
                     KTGAADLFMSRHNHHAKVQGGVLAGECAALLTKFFAGRR"
     gene            2168..2632
                     /locus_tag="VFB53_02230"
     CDS             2168..2632
                     /locus_tag="VFB53_02230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN85580.1"
                     /translation="MNILIDVAAQELRLSGGQRYAVSTAKNGLGEQNGSFCTPRGRHI
                     VRAKIGAGQPLRSVFVRRRPTGEVWTPELHARYPGRDWMLTRILWLSGCEVGRNRLGD
                     VDTMRRMIYIHGAPDSAEMGRPGSHGCVRMRSEDVAELFELVEPYTPVEIRG"
     gene            complement(2605..3564)
                     /gene="mtnA"
                     /locus_tag="VFB53_02235"
     CDS             complement(2605..3564)
                     /gene="mtnA"
                     /locus_tag="VFB53_02235"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZN85581.1"
                     /translation="MTVEALRWNGDSLDLLDQRKLPERIEYLSCRDAAAVAAAIRDMV
                     VRGAPAIGCAAAFGIALEPKESSYGVLAQSRPTAVNLAWALERMRRAADKRAEAEAIF
                     AEDLAVNRAIGAHGAALVPQGARVLTYCNTGALATAGHGTALGVIRSAKDKGVSVVAC
                     ETRPYLQGARLTAWECLQEGIPCTLITDNMAGHLMSRKEVDLVIVGADRIAANGDTAN
                     KIGTYMLAVLARRHALPFYVAAPLSSFDPGITDGSAIPIEERPADEVTGYRGTRWAPN
                     GVAVRNPAFDVTPADLITAIICEKGVVRAPNRERLQPLISTGV"
     gene            complement(3561..5531)
                     /locus_tag="VFB53_02240"
     CDS             complement(3561..5531)
                     /locus_tag="VFB53_02240"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HZN85582.1"
                     /translation="MKSLWNDAEAAQFSGPLGPRVYTSRLLGRDRSLVLHGGGNTSVK
                     LKEKDLFGEEQEILYVKGSGWDLESIEAPGFTPLPLAYLRRLTALQKLSDPQMVSELA
                     THTLRAGAPAPSVETLLHALLPHKYVDHTHADAVLSVSNSPDGEKRIRDIYGDRVVVV
                     PYIMAGFDLAAYCAREFPKQASRDTVGMVLLSHGVFSFAADARASYELMIELVSMAEK
                     YLEAKKAWSVGLPPASAAGFKREAAAQLRHDVSRAAGFPMLCLCNDSPRFLGFARHPD
                     VARISQRGPATPDHVIRTKPLPMLGRDVARFSAAYREYFDRNAKLAKEAKTILDAAPR
                     MALDPELGLAAFGRTAKDTGIVEDLYAQTIDVILRAEALGGWRALEDKHTFDIEYWDL
                     EQAKLKKAGSPPAYAGEVALVTGAASGIGKACAEAFLKRGAAVVGLDRNPAIAGLFQR
                     RDFLGIACDLTDAKTVQAAMDAAVKRFGGIDMLVLNAGIFPTSQAVKDIDAGMWQNAM
                     AVNVEANLLVMQACHALLKLAPRGGRVVVIGSKNVPAPGMGAGAYSASKAALNQLVRV
                     AALEWARDGIRINTIHPNAVYDTALWTEEVLASRAKAYNLTVEQYKKNNLLNTEVSSK
                     DVAEMAAEMCGPLFAKTTAAQVPVDGGTEKLV"
     gene            5555..5977
                     /locus_tag="VFB53_02245"
     CDS             5555..5977
                     /locus_tag="VFB53_02245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN85583.1"
                     /translation="MLRIELMDWAAARAEASRIRTAVFVEEQRVPPEIEMDEHDAACL
                     HALAYAQGRAVGTGRLLPDGHIGRMAVLKESRSLGVGGAILERLVAEARRRGMPQVVL
                     SAQTHARGFYRRHGFREVGAVFEEAGIPHQEMRRALNS"
     gene            complement(5947..6606)
                     /locus_tag="VFB53_02250"
     CDS             complement(5947..6606)
                     /locus_tag="VFB53_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZN85584.1"
                     /translation="MCLILVAWQVHARLPLVVAANRDEFHGRAAARASFWDDKPALLG
                     GRDLQARGTWMGVSRSGRFAAVTNYRGGTEPGAAQSRGALVTRYLEGAGVAELARDKA
                     GYSGFNLLAADGAELWWLSNRDGEPRHLAPGWYGLGNLLLDSPEVAPHLQRFRDCPVA
                     LEPLFGVLEQAKIVDERYGTRCSSLLLMGDEIRFAERGFGSDGGEQSTLRYEFKARRI
                     S"
     gene            complement(6597..6854)
                     /locus_tag="VFB53_02255"
     CDS             complement(6597..6854)
                     /locus_tag="VFB53_02255"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HZN85585.1"
                     /translation="MTSYYVYYRVDAHRVAALREAVQRMFGVIEAATGVRGRWMRRRN
                     EPSTFMEVYEDVADEQPFEALLEREAAVLGLERHVERFQCA"
     gene            complement(6851..7771)
                     /locus_tag="VFB53_02260"
     CDS             complement(6851..7771)
                     /locus_tag="VFB53_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZN85586.1"
                     /translation="MDTKVSHAAAELKRLGMLSCTGDEARAFLHAQLTSDVAGLAADR
                     ARHAGWCSAKGRLQASFLVLPHAQGFLLQVARDIAPAVARRLGMYILRAKAKLADASD
                     AWVQMGLWGAGAAQRLAALGLPVPSGEFAVAHAGDAVVVRVAPLRFLLALPAAQRTRV
                     LDALGDAGDEAWALEEIRAGRALIAQATQDLFVPQMVNFERLGGIDFKKGCYPGQEIV
                     ARTQYRGVLKRRMARARVAAAAAPGDELYAAELPGQASGTVVNAAPAPEGGSEMLAVV
                     QISALEASGALRLGAADGPPLELLPLPDAA"
     gene            7809..8810
                     /gene="mltG"
                     /locus_tag="VFB53_02265"
     CDS             7809..8810
                     /gene="mltG"
                     /locus_tag="VFB53_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZN85587.1"
                     /translation="MTLVRHFFALALLALLAAAGYAAWYVNAPLRFATPSVEVEIPPG
                     TGFRAASGRLQQAGIELGPRRFEALARTLGQDQEIKAGYYGLAAPMTPLQLLDKLARG
                     EVLKAEVQVLEGWTFRELRAALDASGVLRHDSAGLGNAALLKRIGAGEAHPEGLFFPD
                     TYVVARGSSDLALLRHAYRTMQQHLAREWPKRAADTPYRSAYEALVMASIVEKETGRA
                     EEREQVAAVLVNRLRIRMALQADPTVIYGLGEEFDGNLKKVHLRTDGPYNTYVRRGLP
                     PTPIALPGLASLRAALQPARSDALYYVSRGDGTSQFSRTLVEHNRAVSKYQLKPRRR"
     gene            8807..>9278
                     /gene="tmk"
                     /locus_tag="VFB53_02270"
     CDS             8807..>9278
                     /gene="tmk"
                     /locus_tag="VFB53_02270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZN85588.1"
                     /translation="MNRGRFITLEGVDGAGKSTHVAWIAERLRARGHAVLVTREPGGT
                     PLAERLRELVLAQPMDPIAETMLLFAARTDHVGREIAPALAAGTWVLCDRFTDATFAY
                     QGGGKGVAAELIAHLAQVSHNGLLPDLTLVFDCPYEVSRERLRGTGREPDRFERE"
BASE COUNT         1366 a         3246 c         3229 g         1437 t
ORIGIN      
        1 gtccgcgggc gcgaagacca ggaaggccgt caccatcaag tcgcaccaca atgtcggcgg
       61 cctgccgaag tccctcaagc tcaagctgct cgaacctctg cgcgagcttt tcaaggatga
      121 agtgcgcgag ctgggcgtgg cgctcggcct gccgcgcgag atggtgttcc gccatccgtt
      181 tccggggccc ggcctcggcg tgcgcatcct cggcgaggtg aagaaggaat acgccgacct
      241 gctgcgccgc gccgacgcca ttttcatcga ggagctgcgc gccgccggct ggtacgacac
      301 gaccgcccag gctttcgccg tgttcctgcc ggtgaagtcg gtgggggtga tgggcgacgg
      361 ccgcacctac gagtacgtgg tcgccctgcg cgccgtgcag accacggact tcatgactgc
      421 cgcctgggcg ccgctgccgc acgagctgct ggcgaaggtc tccagccgca tcatcaacga
      481 ggtgcgcggc atcaaccgcg ttgcctacga catttcctcc aagccgcccg ccaccatcga
      541 gtgggagtga gcgccggcgg ttacttggcg gcgtccttga ttttgtcgcc ggcttcccgt
      601 atcgcgtcgc cggtcttctt cgcagcgtcg tcgaccgcct ttcctgcctt ttccaccgga
      661 cctttctttt cacaggcggg caatccggcc atcacgcagc ccagcaggat tgcagttgcg
      721 atactgcggt tcagtttcat gttttcctct cgctcgacaa tcctgcagcg tatcgcgcgc
      781 accttcccgg cgatgttcgg tagcgcacgt tgcgtcaatc gtccaaggta tcgcagtcct
      841 gcggccggcc cgaggccatg ccacgcttga accagtgcac gcgctgggcg gatgatccgt
      901 gggtaaacga ttcgggcacc acctggccct gggtctgctt ctgcaggcga tcgtcgccga
      961 tcgcgctcgc cgcattcagc gcctcggcga tgtcgccggg ttcgagttgt ttcagggtgc
     1021 cggcgtggtg tccccacacg cccgcgtagc agtcggcctg cagttccatg cgcacggagg
     1081 cctgattggc ctgggcccgg gagccgcttg ctcgtgcgtc ctgcattttc tggaatttgc
     1141 cggtgagctg ttgcacgtgg tggcccacct cgtgggcgat gacgtaggcc tgcgcaaaat
     1201 cgccaggagc gccgaagcgc gtctggaggt cgcggaagaa tgcgaggtcg atgtagagct
     1261 tctcgtcggc ggggcaatag aaggggccgg tggccgattg ccccatgccg caagccgagc
     1321 gcacctgccc ctcgtagagg accagcgtag ggggccggta ctggctcttc gactgctgga
     1381 agatcccgct ccagacgtcc tcggcgctgc cgaggacctt cgcgacgaac tggcctcctg
     1441 cgtccgcggg tgcgcccttg ggcgctacgc gcgactcgct ctggggcgcg acctgcccgg
     1501 ccatgttcaa cacggccgcg gggtcgaagc cgaggaagta ggcgacgacg gcgatcgcta
     1561 tcccaccgat gcccagcccg ccgccaaggc gcatgcctcg ccggtcctcg acgttggcgc
     1621 tggcccgctg atcgcctagc cgcatatgca gaagagctgg aaagggaatg gcagcttatg
     1681 ctgcctggcc cggacgctct gtgcggcagt gcgcacagcg cggcctgcta gtatcttgga
     1741 cgggaatgac cgacgaagat ttcatgcgcc tcgcgctgga actcgccgcg cgcaccgagg
     1801 ctgaagtgcc ggtgggggcg ctggtggttc tcgggggaaa ggccgtggga gagggctgga
     1861 accggcccct tgccacgcac gacccgacct cgcacgccga gatcgaagcc atccgcgcgg
     1921 caggcaaggc caccggcaat taccgcctgc ccggagcgac gctctacgta acgctcgagc
     1981 cctgcaccat gtgcgtgggc gcgatcttca atgcgcgcat cgcgcgcgtc gtgtacggcg
     2041 cgaccgagcc caagaccggc gccgcggatc tgttcatgtc gcgccataac caccatgcca
     2101 aggtgcaagg cggcgtgctg gccggggagt gcgctgcgct cctgacaaaa ttcttcgcgg
     2161 gccgccggtg aacatcctca tcgacgttgc agcccaggaa ttgcgcctct ccggcgggca
     2221 gcgctacgcc gtctccacgg cgaaaaacgg cctgggagag cagaacggca gcttctgcac
     2281 gccgcgcggg cgccacatcg tgcgcgcgaa gatcggggcc gggcagcccc tgcgctcggt
     2341 gttcgtgcgc cggcgtccga ccggggaggt ctggacgccc gagctgcacg ccaggtaccc
     2401 gggcagggac tggatgctga cgaggatcct ctggctctcg ggctgcgaag tcggcaggaa
     2461 ccgcctgggc gacgtggaca ccatgcgccg catgatctac atccacggcg cgcccgacag
     2521 cgcggaaatg ggcaggcccg gctcgcacgg ctgcgtgcgc atgcgaagcg aggacgtcgc
     2581 cgagctgttc gagctggtgg agccctacac tccggtcgag atcaggggct gaaggcgctc
     2641 ccggttggga gcccgtacca cgcctttttc gcagatgatc gccgtgatca gatcggctgg
     2701 cgtgacgtcg aacgccggat tgcgcaccgc cacgccgttc ggcgcccagc gcgtgccgcg
     2761 gtagccggtg acttcgtcgg cagggcgctc ctcgatcggg atcgccgagc cgtcggtaat
     2821 ccccggatcg aagctcgaga gcggggcggc gacatagaag ggcaaggcgt ggcgccgcgc
     2881 cagcaccgcc agcatgtagg tcccgatctt gtttgccgta tctccgttgg ccgcgatccg
     2941 atccgcgccg acgatcacca ggtcgacttc cttccggctc atcaggtgac cggccatgtt
     3001 gtccgtgatc agcgtgcagg gaatgccttc ctgcaggcat tcccaggccg tcaggcgcgc
     3061 cccctgcaga taggggcgcg tctcgcaggc gaccaccgaa acgcctttgt ccttggcgga
     3121 gcgaatgacg cccagggcgg tgccgtggcc ggccgtggcg agcgccccgg tgttgcagta
     3181 ggtcaggaca cgggcgcctt gcgggaccag tgcagcaccg tgcgcgccga tggcccggtt
     3241 gacggcgagg tcctcggcga agatggcttc ggcctcggcg cgcttgtcgg cggcgcggcg
     3301 catgcgctcc agggcccagg ccaggttgac cgcggtcggc cggctctgcg ccaggacgcc
     3361 gtaggaggat tccttcggct cgagcgcaat gccgaaggcc gccgcgcagc cgatggcggg
     3421 cgcgccgcgc accaccatgt cgcggatcgc cgcggccact gcggcggcat cgcggcagct
     3481 gaggtactcg atgcgctccg gcagcttgcg ctggtcgagc aggtcgagcg aatcgccgtt
     3541 ccagcgcagg gcctcgaccg tcacaccagc ttctcggtgc cgccgtcgac cggcacctgc
     3601 gcggcggtgg tcttggcgaa caatggcccg cacatctcgg cagccatttc agccacgtcc
     3661 ttcgaggaca cctcggtgtt caggagattg ttcttcttgt actgctcgac cgtcaggttg
     3721 taggccttgg cgcgcgaggc gagcacctcc tcggtccaca gcgccgtatc gtagacggcg
     3781 ttggggtgga tggtgttgat gcggatgcca tccctggccc attcgagcgc cgcgacccgc
     3841 acgagctgat tcagcgccgc tttcgacgcc gagtaggcgc cggcgcccat gccgggtgcc
     3901 ggcacgttct tcgagccgat caccaccacg cgcccgccgc gcggggcgag cttgagcaac
     3961 gcgtggcagg cctgcatcac cagcaggttg gcctcgacgt tcaccgccat cgcgttctgc
     4021 cacatgccgg cgtcgatatc cttcaccgcc tgggaggtgg gaaagatccc cgcattcagc
     4081 accagcatgt ctatgccgcc gaagcgcttg accgcggcgt ccatcgccgc ctggacggtc
     4141 ttcgcgtcgg tgaggtcgca ggcgatgccg aggaaatccc ggcgctggaa cagcccggca
     4201 atcgccggat tgcggtccag ccccaccacg gcggcgccgc gcttgaggaa cgcctcggcg
     4261 caggccttgc cgatgcccga ggcggcgccg gtcaccagcg cgacttcccc ggcgtaggcg
     4321 ggcgggctgc cggccttctt cagcttggcc tgctccagat cccagtactc gatgtcgaag
     4381 gtgtgcttgt cctccagcgc gcgccagccg cccagcgcct ccgcacgcag gatcacgtcg
     4441 atggtctgcg cgtacagatc ctcgacgatg ccggtgtcct tcgcggtgcg cccgaacgcc
     4501 gccagtccca gttccggatc cagcgccatg cgcggcgcgg cgtcgaggat ggtcttcgcc
     4561 tccttcgcca gcttcgcgtt cctgtcgaag tactcccggt acgcagcgct gaagcgcgcc
     4621 acgtcgcgcc cgagcatcgg cagcggcttg gtgcggatca cgtggtccgg cgtcgccggg
     4681 ccgcgctgcg agatgcgcgc cacgtcggga tggcgcgcga aaccgaggaa gcgcggcgag
     4741 tcgttgcaca ggcagagcat cgggaagccc gccgcgcgcg acacgtcgtg gcgcagctgc
     4801 gccgccgctt cgcgcttgaa cccggcggcg gaggccggcg gcaggccgac gctccaggcc
     4861 ttcttcgcct cgaggtactt ctcggccatc gacaccagtt cgatcatcag ctcgtaggac
     4921 gcgcgcgcgt cggcggcgaa cgagaacacg ccgtgcgaca gcaggaccat gccgacggtg
     4981 tccctggagg cctgcttcgg gaattcgcgc gcgcagtagg ccgccaggtc gaagcccgcc
     5041 atgatgtagg gcaccaccac cacgcggtcg ccgtagatgt cgcggatgcg cttctcgccg
     5101 tcgggcgagt tcgataccga gagcaccgcg tcggcgtgcg tgtggtcgac gtacttgtgc
     5161 ggcagcagcg cgtgcagcag cgtttcgacc gacggcgccg gggcgccggc gcgcagcgta
     5221 tgggtcgcca gctcgctcac catctgcgga tcggagagct tctgcagcgc ggtcaggcgg
     5281 cgcaggtagg caaggggcag gggcgtgaac ccgggcgcct cgatgctctc cagatcccag
     5341 ccgctgccct tcacgtagag gatctcctgc tcctcgccga acagatcctt ctccttcagt
     5401 ttcaccgagg tgttgccgcc gccgtgcagc accagcgacc ggtcgcgccc gagcaggcgc
     5461 gaggtgtaca cgcgcgggcc gagcgggccg gaaaactgcg ccgcctcggc gtcgttccac
     5521 aggcttttca tgcggcgaat tatacttttg cgccatgctg cgaatcgaac tgatggactg
     5581 ggccgctgcg cgcgccgagg cgagccgcat ccgcaccgcg gtattcgtcg aggagcagcg
     5641 cgtgccgccg gagatcgaga tggacgagca cgacgcggca tgtctccacg cgctcgccta
     5701 cgcgcagggc agggcggtgg gcacggggcg cctgctgccc gacgggcaca tcgggcgcat
     5761 ggccgtgctc aaggagagcc gctcgctggg cgtgggcggt gcgatactcg agcgcctggt
     5821 ggccgaagcg cgccgccggg gcatgcccca ggtggtgctg tcagcgcaaa cgcacgcgcg
     5881 cggcttctac cggcggcacg gctttcgcga ggtcggcgcg gtgttcgagg aagcgggcat
     5941 cccgcatcag gagatgcggc gcgccttgaa ctcgtagcgc agggtggact gctcgccgcc
     6001 gtcgctgccg aagccgcgct cggcaaagcg gatctcatcg cccatcagca gcaggctgga
     6061 gcagcgggta ccgtagcgct cgtcgacgat cttcgcctgc tccagcacgc cgaacagggg
     6121 ctcgagcgcg accgggcagt cccggaagcg ctgcaggtgc ggcgccacct ccggggagtc
     6181 gagcagcagg tttcccagtc cgtaccatcc gggcgccagg tgccgcggtt ccccgtcgcg
     6241 gttcgaaagc caccacagct ccgcgccgtc ggcggcgagc aggttgaagc cgctgtagcc
     6301 ggccttgtcc cgcgccagct cggcaacgcc ggcgccctcc agatagcgcg tcaccagggc
     6361 gccgcgcgat tgcgcggccc cgggctcggt gccgccgcgg tagttggtga ccgccgcgaa
     6421 acggccgctg cgcgaaacgc ccatccacgt cccgcgcgcc tgcaggtccc ggccgccgag
     6481 cagcgcgggc ttgtcgtccc agaaggacgc gcgcgcggcg gcacggccgt ggaactcgtc
     6541 ccggttcgcg gcgaccacca gcggcaaccg cgcgtgcacc tgccaggcga cgaggatcag
     6601 gcacattgga agcgttcgac gtggcgttcc aggcccagta ccgcggcttc ccgctcgagc
     6661 agggcctcga acggctgctc gtcggcgacg tcctcgtaga cctccatgaa ggtcgacggc
     6721 tcgttgcgcc ggcgcatcca gcgcccgcgc acgccggtgg ccgcctcgat gacgccgaac
     6781 atccgctgca ccgcctcgcg cagcgcggcg acccggtgcg cgtcgacccg gtagtagacg
     6841 tagtagctgg tcacgccgcg tcgggcaggg ggagcagctc gagcggcggc ccgtcggcgg
     6901 cgccgaggcg cagggcgccg gaggcctcca acgcgctgat ctgcaccacg gcgagcattt
     6961 cgctgccgcc ctccggcgcg ggcgcggcgt tcaccaccgt gccgcttgcc tggcccggga
     7021 gttccgcggc gtacagctcg tcgccgggcg cggctgccgc ggcgacccgc gcgcgggcca
     7081 tgcggcgctt gagtacgccg cggtactgcg tgcgcgccac gatttcctgc cccgggtagc
     7141 agcccttctt gaaatcgatc ccgccgaggc gctcgaaatt caccatctgc ggcacgaaca
     7201 ggtcctgcgt cgcctgcgcg atcagcgccc gcccggcgcg gatctcttcc agcgcccagg
     7261 cctcgtcacc ggcgtcgccc agcgcatcga gcacgcgcgt gcgctgcgcc gccggcagcg
     7321 ccagcagaaa gcgcaggggt gccacgcgga ccaccacggc atcgcccgca tgcgccaccg
     7381 caaattcgcc cgacggcacc ggcaggccca gggccgccag gcgctgcgcc gcgcccgcgc
     7441 cccagaggcc catctgcacc caggcgtcgc tcgcatcggc cagcttcgcc ttggcgcgca
     7501 ggatgtacat ccccaggcgc ctggcgacgg cgggcgcgat atcgcgcgcc acctgcagga
     7561 ggaatccctg cgcatggggc agcacgagga agctggcctg cagcctgccc ttggcggaac
     7621 accagccggc gtggcgtgcg cggtccgcgg ccaggccggc gacgtcgctg gtgagctgcg
     7681 cgtgcaggaa cgcgcgcgcc tcgtcgcccg tgcacgacag catgccgagc cgcttcagtt
     7741 ccgccgccgc gtgggaaacc ttggtgtcca tcgtagaatg ccggtggaga ttttattccc
     7801 tttccgcgtt gaccctcgtt cggcacttct tcgcgctggc actgctcgcg ctgctcgccg
     7861 cagcgggcta tgccgcctgg tatgtgaacg cgcccctgcg tttcgccacg ccgtcggtgg
     7921 aggtcgaaat cccgcccggc accggttttc gcgcggccag cggccgcctg cagcaggcgg
     7981 gcatcgagct gggtccgcgg cgcttcgagg ccctggcgcg cacgctggga caggaccagg
     8041 agatcaaggc cggctactac gggctggcgg cgccgatgac gccgctgcag ctgctcgaca
     8101 agctggcccg tggcgaggtg ctgaaggcgg aggtccaggt gctcgaaggc tggacattcc
     8161 gcgagctgcg cgccgcgctc gatgcgagcg gcgtgctgcg ccacgactcg gcagggctgg
     8221 ggaatgcggc tctgctcaag cgcatcggcg cgggcgaggc gcatcccgag ggcctgtttt
     8281 ttcccgacac ctacgtcgtg gcccgcggct ccagcgacct cgcgctgctg cgccacgcct
     8341 accgtacgat gcagcagcac ctggcgaggg aatggccaaa gcgcgccgcc gacacgcctt
     8401 accgcagcgc ctatgaggcg ctggtgatgg cctcgatcgt cgagaaggaa acgggcaggg
     8461 cggaggagcg cgagcaggtt gccgcggtgc tggtcaaccg cctgcgcatc cgcatggcgc
     8521 tgcaggccga tcccaccgtg atctacggcc tgggcgagga attcgacggc aatctgaaga
     8581 aggtgcacct gcgcaccgac ggcccctaca acacctatgt gcggcgcgga ttgccgccga
     8641 cgccgatcgc gctgcccggc ctggcgtcgc tgcgcgccgc gctgcagccg gcgcgcagcg
     8701 acgcgctgta ttacgtatcg cggggcgacg gcaccagcca gttctcgcgc acgctggtgg
     8761 agcacaatcg cgcggtgtcc aaataccagc tgaaaccgcg gcgccgatga accggggacg
     8821 cttcatcacg ctggaaggcg tggacggcgc cggcaagtcc acgcacgtgg cctggatcgc
     8881 cgagcggctg cgcgcgcgcg gccacgccgt gctggtcacg cgcgagcccg gcggcacgcc
     8941 gctcgccgag cgcctgcgcg agctggtcct cgcgcagccc atggacccga tcgccgagac
     9001 catgctgctg ttcgcggcgc gcaccgacca cgtggggcgc gagatcgcgc ccgcgctggc
     9061 ggcggggacc tgggtgctgt gcgaccggtt caccgatgcc accttcgcct accagggcgg
     9121 aggcaaaggg gtggccgccg agctgatcgc ccacctggcg caggtgtcgc acaatggcct
     9181 gctgccggat ctgaccctgg tattcgattg cccgtacgaa gtgagccgcg agcgcctgcg
     9241 cggcacgggc cgcgagcccg accgcttcga acgggaag
//
LOCUS       DASRIE010000051         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2928492, whole genome shotgun sequence.
ACCESSION   DASRIE010000051 DASRIE010000000
VERSION     DASRIE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928492"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1292
                     /gene="glgC"
                     /locus_tag="VFB53_02275"
     CDS             90..1292
                     /gene="glgC"
                     /locus_tag="VFB53_02275"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZN85589.1"
                     /translation="MVLAGGRGSRLKELTDWRCKPAVPFGGKYRIIDFTLSNCVNSGV
                     RRIGVATQYKSHSLIRHLQRGWSFLDGRIGEQVDILPAQQQVAESWYRGTADAVFQNL
                     NVLRRDGCSYVLVLSGDHVYKMDYGRLLEDHAAREADATVVCVEVPLAEARSFGVLQV
                     DAAGRVVGFEEKPAEPRPMSGAAGRALASMGVYVFNAAFLYEQLVRDADDPRSSHDFG
                     KDLIPHIISRYRVHAHRFAESCVETQSGHPYWRDVGTLDAYWEANLELAKITPQLNLY
                     DRDWPVWTFQEQLPPAKFVFDDEGRRGMAVDSMVSGGCIVSGATVRRSVLFSNVRVEE
                     GALLEDCVVLPDVVVGPGVRLRGAVVDRHCRLNAELLEDPRRFRFTEQGLMLVTPEML
                     GQQVHQLR"
     gene            1469..1654
                     /locus_tag="VFB53_02280"
     CDS             1469..1654
                     /locus_tag="VFB53_02280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN85590.1"
                     /translation="MRRGAASSSRSDGNAIRASVSVGASEYPGDGEEADRLRKRADEA
                     MYAAKHAGGNGYRFYGR"
     gene            complement(1642..1800)
                     /locus_tag="VFB53_02285"
     CDS             complement(1642..1800)
                     /locus_tag="VFB53_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85591.1"
                     /translation="MKPWQHDQLRRALEQRRAGLLEELGRDAEELRAIQAARCVECQA
                     RHEKTYRP"
     gene            1884..>2443
                     /locus_tag="VFB53_02290"
     CDS             1884..>2443
                     /locus_tag="VFB53_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85592.1"
                     /translation="MASPFEPHLLAAYLGAVHGAPVVDVRVTPLGGGRQGDKGYGYGV
                     PLRVDYTLGGAPRRAVIETVRAGPFGHEHMADRAQSLLWAHDAFQRLPRHVTSLDVGA
                     VRSSGALVPLGNAEELFMLAEFIDGREYADDLLRLRAGGELSTGDLDRADALCDYLVE
                     IHRVRGPDPGLYVRRVRELIGHGECV"
BASE COUNT          337 a          854 c          870 g          382 t
ORIGIN      
        1 ccatgggcgt agccgaaaca ctgtcccgta cggtcaaggc ggagctcggc cgcaggcatt
       61 tcggccgact tgcgcgcagc acctatgcga tggtgctggc gggcgggcgg ggcagccgcc
      121 tgaaggagct caccgactgg cgctgcaagc cggcggtgcc gttcggcggc aagtaccgca
      181 tcatcgattt cactctctcc aactgcgtca actcgggcgt gcgccgcatc ggcgtcgcca
      241 cccagtacaa gtcgcacagc ctgatccgcc acctgcagcg cggctggagc ttcctcgatg
      301 ggcgcatcgg cgagcaggtc gatatcctgc ccgcgcagca gcaggttgcc gagagctggt
      361 accgcggcac cgccgacgcg gtgttccaga acctcaatgt cctgcgccgc gacggctgca
      421 gctacgtgct ggtgctctcc ggcgatcacg tctacaagat ggactacggc cggctgctcg
      481 aggaccatgc ggcgcgcgag gccgacgcca ccgtggtgtg cgtggaggtg ccgctcgccg
      541 aggcgcgcag cttcggcgtg ctccaggtcg acgccgccgg gcgcgtggtc ggcttcgagg
      601 agaagccggc cgagccgcgg ccgatgtccg gcgccgccgg gcgcgcgctc gccagcatgg
      661 gcgtatacgt gttcaacgcg gccttcctct acgagcagct ggttcgcgac gcggacgacc
      721 cgcgctcgag ccacgatttc ggcaaggacc tcatcccgca catcatctcc cgctaccggg
      781 tgcacgcgca ccgcttcgcc gagagctgcg tcgagacgca gagcggccac ccctactggc
      841 gcgacgtcgg cacgctcgac gcgtactggg aagcgaacct cgagctcgcc aagatcacgc
      901 cgcagctcaa cctctacgac cgcgactggc cggtgtggac cttccaggag cagctgccgc
      961 cggccaagtt cgtgttcgac gacgaggggc ggcgcggcat ggcggtcgac tcgatggtct
     1021 cgggcggctg catcgtgagc ggtgccaccg tgcgccgctc ggtgctgttc tcaaacgtgc
     1081 gcgtggaaga gggcgcgctg ctcgaggact gcgtggtgct gcccgacgtg gtggtcgggc
     1141 cgggggtgcg gctgcgcggt gcggttgtcg atcgccactg ccggctgaat gcggagcttc
     1201 tcgaggaccc gcggcgcttc cgcttcaccg agcaggggct gatgctggtc acacccgaga
     1261 tgctcggcca gcaagtgcac cagctgcgct gagcaacatc cgctttttca tcggtcctct
     1321 tcagttcgtg gcgatgcgcg ccgcgcgcga ccgcacactc ggaacgagtg gccgccctgg
     1381 tgcggggcgc agggctagcg cgtgccgccg ctgaccgatc tgcccaaccg gggcctgttc
     1441 cgcgatcgcc tcggccaggc gctgcgccat gcggcgcggc gcggcgagca gttcacgctc
     1501 ggacggcaac gcgatccgtg cctcggtcag cgtcggcgcg agcgagtacc cgggtgacgg
     1561 ggaggaagcc gaccgcctgc gcaaacgcgc cgacgaggcg atgtacgccg ccaagcacgc
     1621 gggcggcaac ggctatcgct tctacggccg gtaggtcttc tcgtgccgcg cctggcactc
     1681 gacacagcgc gcggcctgga tcgcacgcag ctcctcggcg tcgcgaccga gctcctcgag
     1741 caggcctgcg cggcgctgct cgagcgcgcg gcgcaactgg tcgtgctgcc aaggtttcat
     1801 gcacacaggt tatcgccgcc ccggtcgcgg tttgaccgag atcaaatcgc gccgggcgcc
     1861 gcccggcgta gcgtaggcgt ctcatggcct ctcctttcga gccgcacctg ctggcggcgt
     1921 acctgggcgc ggtgcatggc gcgccggtcg tcgacgtgcg cgtgacgccg ctcggcggcg
     1981 ggcgtcaggg cgacaagggg tacggctacg gcgtcccgtt gcgcgtcgac tacacgctag
     2041 gcggtgcgcc gcggcgcgcc gtgatcgaga ccgtgcgcgc cggcccgttc ggccacgagc
     2101 acatggcgga ccgcgcgcag tcgctgctct gggcgcacga cgcgttccag cggctgccgc
     2161 gccacgtcac ctcgctcgac gtcggcgcgg tgcgctcgag cggcgcgctg gttccgctcg
     2221 gcaacgccga agagctgttc atgctcgccg agttcatcga tgggcgggaa tacgccgacg
     2281 atctgcttcg cctgcgcgcg ggcggcgagc tttccacggg tgacctggac cgggcggacg
     2341 cgctttgcga ctacctggtc gagatccatc gcgtgcgcgg ccccgatccc ggcctctacg
     2401 tgcgccgcgt gcgcgagctc atcggccatg gcgaatgcgt gtt
//
LOCUS       DASRIE010000052        26993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_827535, whole genome shotgun sequence.
ACCESSION   DASRIE010000052 DASRIE010000000
VERSION     DASRIE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 26993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827535"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFB53_02295"
     CDS             <1..554
                     /locus_tag="VFB53_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZN85593.1"
                     /translation="DPKLPVEQEAASGTLITFEEDPHMEKAIVSGVAAQRDEAKVTVH
                     GVPDKPGIAYAILGPIADAGINVDVIVQNVSHDGMTDLSFTLNRGDLKRAMKMLNDKV
                     KPAIKCREITGDDHMAKVSVVGLGMRSHAGIAAKMFRTLAEEGINIQMISTSEIKISV
                     VVDEKYAELAVRVLHKAFELDQG"
     gene            569..661
                     /locus_tag="VFB53_02300"
     tRNA            569..661
                     /locus_tag="VFB53_02300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Ser,seq:gct)
     gene            complement(651..1271)
                     /locus_tag="VFB53_02305"
     CDS             complement(651..1271)
                     /locus_tag="VFB53_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259447005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1224..1226),aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HZN85594.1"
                     /translation="MATEPLTLEIFSDFVUPWCYLSTVRIERLRKEHGVRVKWIHFPL
                     HPETPAAGRSLEDLFAGRGYDIPKMQAQMRARMEAEGLPYGTRTMTYNSRLAQELAAW
                     ADTQPGGEAIHHAMFRAYFVEARNIGDAEVLVDIAGSVGLPPDQAREVIAKRTHQAAV
                     DADWEKSRRYGVTGVPTFVVGNQGVVGAQPYEVLEELVRQGKRLAE"
     gene            1354..2148
                     /locus_tag="VFB53_02310"
     CDS             1354..2148
                     /locus_tag="VFB53_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN85595.1"
                     /translation="MLKTYATLALKDEGPHVLVVTLNRPEVLNAISTQMGRDWLDLFN
                     GLAADPGDTRCIVLTGAGERAFCAGADLKERDGMAVETWRTQHAQFEQAFVALLECPV
                     PMIAAVNGHAYGGGLETALCCDFIYAAKSAKLALSELRLGIMPGGCGTQNLPRAVGER
                     RAKELILTARAFTAEEGAAWGLVNRVCEPGRTLADALETAQQIAANAPLAVRQAKKSI
                     HYGLQMDLLSGYRFEIEAYNRLVDTEDRREGVRAFNEKRKPSFKGR"
     gene            complement(2145..2882)
                     /locus_tag="VFB53_02315"
     CDS             complement(2145..2882)
                     /locus_tag="VFB53_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZN85596.1"
                     /translation="MFTNREGQRVPQVSFRTRSNGEWKTVTTDEIFKSRTVVVFSLPG
                     AFTPTCSSTHLPRYNELAPAFRANGVDEIVCISVNDGFVMSEWAKEQEAANVRLVPDG
                     NGEFTEKMGMLVDKAELGFGKRSWRYSMLVQDGVVRKMFIEPQKAGDPFEVSDADTML
                     AWINPKAKKPDQVAILTREGCSFCAKAKALLTERGYDYAEIALDHKLRTRVVGAIAGA
                     QTVPQVFINGELIGGWEALERWTQKAA"
     gene            3011..3547
                     /locus_tag="VFB53_02320"
     CDS             3011..3547
                     /locus_tag="VFB53_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN85597.1"
                     /translation="MAKPVEVNQFKLGMRSLAHAVNIVTAAHGGHRCGMTATAVCSAT
                     AEPPTVLVCLNRASATYAGVTKSKSFCVNVLRAEDSDLSNLFSGAQSGEGRFKSREWT
                     RLATGSPVLLDALVSFDCRVIKKLAHGSHTVFLGQVEQVLFGKKGRPLLYAEGQYSKL
                     ASLAHGEPLPEGLDYWGF"
     gene            3576..3652
                     /locus_tag="VFB53_02325"
     tRNA            3576..3652
                     /locus_tag="VFB53_02325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3610..3612,aa:Arg,seq:acg)
     gene            3734..4546
                     /locus_tag="VFB53_02330"
     CDS             3734..4546
                     /locus_tag="VFB53_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZN85598.1"
                     /translation="MRKLRSAPPGLVALGALLALAALWLAVNGLVQVVRKPSELFFPV
                     SGVLHKSPTETWRHYEPMFRAHATAVVTADLLAALAQVESAGNPVARTNWRWRLTEDP
                     FDVYRPASSAVGMFQITDGTFRQARRYCIHHHAVVEDGPWHDWDSCWFNSFYFRVVPS
                     HAAEMTAAFLDRSVARVLARQKVAKATLRQKQELAIVMHLCGAGAGEVYARGGLRLAA
                     NQRCGGQDVHAYVERVRAMQAQFARLAERAAAPLHLASGTGTGYPSRFKEAA"
     gene            4543..5514
                     /locus_tag="VFB53_02335"
     CDS             4543..5514
                     /locus_tag="VFB53_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85599.1"
                     /translation="MTSLARGLVAAFAALWLASAAAQQAYPTKTIRFIVTFPPGGSSD
                     LIARAIAPVLSDRLRQSVIVENRPGAGGNIGMEMLAKSAPDGYTMGLGAAGALAANVS
                     LYPKLPYDPRKDFAPVSNVAFVPFFIIAHPSLPVNNLEELIALARAKPGELLLGYGGN
                     GTAMHLSGELFKLMAKVQMVNVPYKGSGPAAQDTLGGQLPLAVVDVASALAQVKAGRL
                     KALAVTSAKRIGAAPQVPTVAEAGLPGYDATGWFGVVMPAGTPATVIGRMNAELVAAL
                     KRADIRDRVIAAGAEPSPSTPAEFGELIRTEIDKWAEVVKLSGARVD"
     gene            5517..7205
                     /gene="ilvD"
                     /locus_tag="VFB53_02340"
     CDS             5517..7205
                     /gene="ilvD"
                     /locus_tag="VFB53_02340"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZN85600.1"
                     /translation="MARRKIPILPSRVTVEGLDRAPHRAFLRSLGVHGADLGKPFVGV
                     ASTDGRVTPCNALLGEFAREACAGVRAAGGVPFDFASISVADSMSMNHAGMRYSLVSR
                     ELIADGVEAVVRGHAYDALVAFAGCDKTLPGMMMAMVRLNLPAVFVYGGAALPGRWRG
                     RDVTVLDTYEGVGAVMAGDLPQAQLDELEAVCLPTLGSCPGQFTANTMAMVSETLGLA
                     QPGTATLPAVAEGRSAAAREAGAAVMRLLREGGPLPRELVTRKSIENACAAVAATGGS
                     TNAALHLPAIAHEAGIRFDLDDFARIARRTPLIADMKPGGRYLAKDLHAAGGVYSVLK
                     ELLRRGALHGDCLTLSGRTLEQALEGQGGADGEVVHREPLSPDGGLVVLKGNLCPDGG
                     LIKVAGLKSLVFDGRARVFDSEEACAAQVRARKLRPGDVLVIRYEGPRGGPGMREMLG
                     VTALIYGQGLGEKVALVTDGRFSGATRGMMVGYVCPEAAAGGPIALVRNGDRITIDAR
                     KGRATLQVSKAELAVRRRAWRAPRQAKLSGALEKYARLVGPANRGAVTHSGGGI"
     gene            7214..8089
                     /locus_tag="VFB53_02345"
     CDS             7214..8089
                     /locus_tag="VFB53_02345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85601.1"
                     /translation="MHLGFIGTGLMGRPMVLRLLAAGYPVSVWNRSAAKLEPLLSEGA
                     LAQSSPAAVARAADIVMMCVTDQGAAGEVLFGPDGVAAGGAAGKLVVDFSSIAPASAR
                     EFAGRLERECGMGMVDAPVSGGTRGAEQGTLAIMAGGRPEHIERVRPVIAHLAQRFTR
                     MGEAGAGQVTKLCNQVIVGSLLPVIAEAVRLAEAAGVDANSLPGALQGGFADSLPLQV
                     FGARMAARNFDPPLGSVAIMLKDLENAATVAKQTGVPLPMAHCAAELYRLLVAQGRAE
                     HEPSALVELLEGKRA"
     gene            8181..8468
                     /locus_tag="VFB53_02350"
     CDS             8181..8468
                     /locus_tag="VFB53_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZN85602.1"
                     /translation="MRSVTKADLVEAAARAGNLSKSSAGEAVNAVFDAIVSGVAKGNR
                     VTVVGFGTFLPRKRKARAGRSPLNGKEIRISSKTVPAFAAGQGFKDRVDNR"
     gene            8518..9096
                     /locus_tag="VFB53_02355"
     CDS             8518..9096
                     /locus_tag="VFB53_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZN85603.1"
                     /translation="MDHLLILTLDSHGVPHRWVSWQQACFYYAKNLVAWTLGERTFTF
                     RGGISRLTGERSSITAHSIIAIRGKALAAKGFNQVPPLNNRELFRRDRHTCAYCGHEY
                     GYFRLTRDHITPLSRGGRDTWMNVVTACRHCNGHKRNRLPEESGLELMYAPYVPNKAE
                     YLILTNRRILADQMEFLRQHVAAHSRLVLAPA"
     gene            9125..10096
                     /locus_tag="VFB53_02360"
     CDS             9125..10096
                     /locus_tag="VFB53_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HZN85604.1"
                     /translation="MQKDAAGRLAVTDDFLRAFYLRPEVHPVEESCDAELRLHAGLMA
                     APRRAVGAAEIDAVADEDARHNYRVLLAFRDRLLAAGTVEGCYMGLFKGAIDVPPLFI
                     EQLAHVIVRNLLQDCDDPLRLRAAELFFREQKATLQDGHVLLADLETVATHAAGNRYG
                     NLGKLIVEAQGTVGTVDLDVLDRANAALYWERESRHDTVISLTYGRPALDALCRVLEA
                     WVAHFHGLQVSVKPLRHIEEGRWAWHIGLDAESTEILNALWAGGQVDPGRLRNIVALF
                     GLQFSDPAAMRQDIAGRTVYLALSANADQEVRMKPQNLLTNLPLHEA"
     gene            10086..10703
                     /locus_tag="VFB53_02365"
     CDS             10086..10703
                     /locus_tag="VFB53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZN85605.1"
                     /translation="MKPEYWDRAKRALARRDPVLAAIMRRHPRVHLVARGEPFQTLAR
                     AIVGQQISVKAAQSVWNRVLEVAPAMTPAAVLAVRRPRLRSCGLSDRKTEYIADLARH
                     FAEGTVHPHRWPQMDDEAVIAELVAVRGIGRWTAEMFLMFNLLRPDVYPLGDLGLLNA
                     LRRSYFNGRKAPLSRLRKLGQAWQPWRSVATWYLWRSLDPEPVEY"
     gene            10755..11843
                     /locus_tag="VFB53_02370"
     CDS             10755..11843
                     /locus_tag="VFB53_02370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN85606.1"
                     /translation="MPIRFFPEDRDQALRVRRYLIASGTTLLVVLVYCVCYWAGLMAW
                     PVLAAGIAFAAVRAVLFYALFRTGANRRFRDPSLTTEQIVFATLNTALVMFFAEQARP
                     ALLPIYVVPFLFGVFRLRTREFLGLGLVVLLIFAAMQYVSAHLGFARVDAARDMVDFV
                     VVAVVVPWFAVFGGYVNGLRTELGGANRKLKAALERIEKIAIRDELTGVYNRHYLAEL
                     LQQEKARFDRGGAVFSVCMLDIDHFKAVNDAHGHGAGDAVLRQFAETVQSAMRRADVF
                     GRYGGEEFLLVMPQTALDGAAASAERIRAAVESCACAALPAGRRVTVTIGVATIAAGE
                     DNAALLARADECLYRGKAAGRNRVVAVG"
     gene            11854..12822
                     /locus_tag="VFB53_02375"
     CDS             11854..12822
                     /locus_tag="VFB53_02375"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZN85607.1"
                     /translation="MKTTFLDFEQPIAELEQRIEELRFVQDDSAVDISEEIQRLTKKS
                     QALTKEIYAKLTPWQVAQVARHPQRPYTLDYVGMLFAGYEELHGDRTFADDASIVGGL
                     ARFDGLPCMVIGHQKGRDTKEKIARNFGMPKPEGYRKALRLMKLAEKFSLPVFTFVDT
                     PGAYPGIDAEERGQSEAIGRNLYAMAALRTPIIASVIGEGGSGGALAIAVGDVTLMLQ
                     YATYSVISPEGCASILWKSADKAPEAAETLGITANRLKTLGLVDKVVSEPLGGAHRDP
                     QAAGQALKKALAEALRQLREKKPKELVEERLERLMSYGKYKEAAER"
     gene            12791..13957
                     /gene="tilS"
                     /locus_tag="VFB53_02380"
     CDS             12791..13957
                     /gene="tilS"
                     /locus_tag="VFB53_02380"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZN85608.1"
                     /translation="MASTRKPPSVDAAGLALRGRRVAVGLSGGVDSVVLLHLLRDAAP
                     QLGFVLSAVHVHHGLSPNAGAWARFCRKLCRAWGVPLTVRRVRVRRAGQGLEAAAREA
                     RRAVFAGLPAEVVALAHQLDDQAETVLMNLLRGTGLRGASAMPAAGRLGRKILWRPLL
                     AVPRRALLAHARAQRLQWVEDESNGDEALTRNFLRRRVGPLLEARFPRWRESLARAAR
                     HFASAEASAERALRAFLGAQGLRSPSAAKLVEMLRQLTGRGARTAIAHDGAVLRAYRG
                     RVSVERPGAPAEEVRLRPARGRGIDAARLERGRVSLRLRAGGERLQLDPRRPRRTLKN
                     LFQEAGVAPWQRERLPLLYCDDELVWVPQLGIAAGWHAPRGARGLVPEWRPAAA"
     gene            14039..14464
                     /gene="ndk"
                     /locus_tag="VFB53_02385"
     CDS             14039..14464
                     /gene="ndk"
                     /locus_tag="VFB53_02385"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZN85609.1"
                     /translation="MAVQRTLSIIKPDAVKKNVIGKIIARFESAGLRIAAARMMHLSR
                     AEAEGFYAVHRERPFFKDLVDFMISGPVLVQVLEGRDAIAVNRELMGATDPQKAAKGT
                     IRADFADSIDANAVHGSDGPETARSEIAYFFPACEVFAL"
     gene            14466..15542
                     /gene="rlmN"
                     /locus_tag="VFB53_02390"
     CDS             14466..15542
                     /gene="rlmN"
                     /locus_tag="VFB53_02390"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZN85610.1"
                     /translation="MKTNLLGLDAAGLAAYFQTLGEKPFRARQVMQWVHQRREGDFAR
                     MTELAKAMRAKLAQDATVAAPQIVGDHVAEDGTRKWLLKVDGANAVDAVFIPETSRGT
                     LCVSSQAGCTLDCAFCATGKQGFSRNLTTAEIVGQLWLANRLLGGERPVTNVVLMGMG
                     EPLLNLDQVIPALRLMLDDNAYGLSRRRVTVSTSGVVPGMDRLRDECPVALAVSLHAP
                     NDALRDRLVPVNRKYPIRELLQACRRYLERAPRDFITFEYVMLDGVNDSDEQARELAA
                     LARRVSCKFNLIPFNPFPATEFRRSPPERIRRFAEILQRAEVTVTTRKTRGDAIDAAC
                     GQLAGEVTDRTRRSKVLRVAELRS"
     gene            15539..16318
                     /gene="pilW"
                     /locus_tag="VFB53_02395"
     CDS             15539..16318
                     /gene="pilW"
                     /locus_tag="VFB53_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZN85611.1"
                     /translation="MSRALWLVLALCAAGCATQAPEGPASGAGAIVGDAGTPRNRARV
                     HTELATLYYARGNMGVALDEARVAVAADASYATAHGVLGLVYMELRENDLARQSFERA
                     LSHAAEDPDINHNYGWFLCQTGKEGEAQPYFRRALANPLYATPARSYAAAGTCALRIG
                     RLAEADDNLGRALTIDPSQPTALLQSALLRYRQGRFDEARRLMARQAQVAEATPESLW
                     LALRIERRRGARADELSYANRLRRMFPDSPQYQALRRGEYD"
     gene            16311..17147
                     /locus_tag="VFB53_02400"
     CDS             16311..17147
                     /locus_tag="VFB53_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZN85612.1"
                     /translation="MTSAQAGAAAPASDGREPVGSVLARAREAAGLSLEDAARQLRFA
                     PRQLEALERDALDRLPGGAAVRGMVRSYARLLRIDAEPLVAQLAADARAQDAERLAQR
                     FRQPVPFSDGSRRSNLAYIALSLTLLAVVAAVAFEWRQERGTERLTFVSAAQQPAEPV
                     RVAAASAATPPAPAPTAPPEAARPVRAPVAAAEDGDGEAGRGRIVLQFERESWVEIRS
                     GSGKLLVSQLHAPGADRVVSGVPPFAVVIGNAQHVRLSYNDKPVDLMPHVKVEVARFT
                     LE"
     gene            17150..18406
                     /gene="ispG"
                     /locus_tag="VFB53_02405"
                     /gene_synonym="gcpE"
     CDS             17150..18406
                     /gene="ispG"
                     /locus_tag="VFB53_02405"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZN85613.1"
                     /translation="MGARRPSSRVMVGPVAIGGGAPVVVQSMTNTDTADAEATARQVA
                     ELWRAGSELVRITVNSPEAAAQVAGIRARLDAMGCDVPLIGDFHFNGHKLLTQFPDCA
                     WALAKYRINPGNVGKGAKRDEQFATMIEAALRHGKPVRIGVNWGSLDQALLARLMDEN
                     ASRAEPLDAAEVMREAVILSALESARQAEAWGLPAERIVISCKMSGVQDLIGVYRELA
                     RRCGYALHLGLTEAGMGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGGDRTREVVV
                     AQEILQTLGIRSFTPMVAACPGCGRTTSTLFQELAARIQSYLRAQMPVWRERYPGVED
                     MRVAVMGCVVNGPGESKHADIGISLPGTNELPVAPVYVDGAKTVTLKGGRIAEEFQAL
                     VEEYVRKNYAPGAARARPRHIPVKAA"
     gene            18440..19684
                     /gene="hisS"
                     /locus_tag="VFB53_02410"
     CDS             18440..19684
                     /gene="hisS"
                     /locus_tag="VFB53_02410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZN85614.1"
                     /translation="MNDVLPGDAELWLWFEGRVAGWFGRYGYRQIRTPLLEHTGLFRR
                     AIGEVTDIVEKEMYTFTDALNDESLTLRPEATASTVRAAIEHNLLYEGPRRLWYQGPM
                     YRHERPQKGRYREFHQFGAEALGYAGPDVDAELIVMCAALWRELGLQGIRLELNTIGS
                     AAERARYRADLVRHFEAHAPALDADAQRRLHSNPLRILDSKNPRVQEVAAQAPELVKY
                     LGDASLGHFEGLRRLLAASGVDYKVNPRLVRGLDYYNLTVFEWITDRLGAQGTVCGGG
                     RYDGLCAQLGGKPAPGVGFAAGVERLIALLRDAGRAAAAPPPHAYLVHHGEGTLPYAF
                     TLAEELRAAGLAVVLHAGAGNIKAQMKKADASGALLALIVGEDELKAAAVTVKPLRDA
                     RPQERAPRDALAGRLQEILKGS"
     gene            19688..20326
                     /locus_tag="VFB53_02415"
     CDS             19688..20326
                     /locus_tag="VFB53_02415"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN85615.1"
                     /translation="MALQNLDLEEQEKLEELKAWWRQYGNLILMAITAVAIAVAGWGA
                     WRWYQRNEAQQASRLYESLAAAARAGDAKALRDAGGALTEGYPRTLHASMGALASARF
                     HFDRGELKSARAQLQWVIERSRSEDLRDLARLRLAALLLDEKEYDAALKLLEAKPLEA
                     YAAQYAALQGDVLAAKKQPAEARAAYQRALDKAGEGGAFRDSVQMRLDALGG"
     gene            20329..21453
                     /gene="bamB"
                     /locus_tag="VFB53_02420"
     CDS             20329..21453
                     /gene="bamB"
                     /locus_tag="VFB53_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HZN85616.1"
                     /translation="MSRHAALVLLLPALAGCFGSAPAGPQPAELPALEPAREPRQLWS
                     AAVGAAGAFAFQPALAGDSIYAASRNGAVARLEAAGGRERWRIALDAKLSGGVGADAR
                     TVVVASDRGEVFALEAADGKLRWRARVSSEVLAAPRVHGDLVLVRSADSRVYAMGAED
                     GKRRWAYQRAPSALMVRSPAGMVPQADTLYVGFAGGRLVALALSNGGLRWEAAVAQPR
                     GATELERVADVVGDPVVIGREVCAAAFQGRVACFDAQNGSQLWARELSSVTGVSADAR
                     TAYVSDDRGAVHALDRSNGRSLWKQDRLAHRQLSLPVPLGEQIAVGDLQGYVHLLSRD
                     TGAFVGRAGTDGSPVRAAPLPLPEGFLVQTQRGGLFALAP"
     gene            21467..22843
                     /gene="der"
                     /locus_tag="VFB53_02425"
     CDS             21467..22843
                     /gene="der"
                     /locus_tag="VFB53_02425"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZN85617.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRRRDAIVVDVPGVTRDRHYGE
                     GRLGERPFIVIDTGGLELRAREGIYVEMARQAEQAMAEADAIVFLTDGREGLHDGDAE
                     IARRLRKLKARVWLVVNKTEGMASDAAVAEFHALGLGQPQPISSAHGDGVGDLIDGVL
                     AEFPRADEEAQEARDKHPRVAIVGRPNVGKSTLVNALVGEERVIAFGEPGTTRDAIEV
                     PFERAGRHYTLIDTAGLRKRGKQGEATEKFSIVKTLQAIEQSNVAVLVLDAAEGVSEQ
                     DAHVAGYILERGRALVVAVNKWDATQREARDRIKQELAWKLVFLGFAETHYLSAKSGK
                     GLGELMRSVDGAYAAAMAKLSTPKLTRALIAAVERQPPPRKGLRRPKLRYAHQGGQNP
                     PRIIIHGNSLDALPDSYRRYLEGVFRDGFALVGTPMRVEFRTGRNPYAGRGPAPQHRK
                     LSEKRGGR"
     gene            complement(22865..25867)
                     /locus_tag="VFB53_02430"
     CDS             complement(22865..25867)
                     /locus_tag="VFB53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85618.1"
                     /translation="MPRFVPSRTAFYLLFTVSGFAGLIYESIWTHYLKLFLGHAAYAQ
                     SLVLVVFMGGMALGAAVCGRVSTRLRNPLAGYALVEAVVGLCALGLHEVFIAVTDWSY
                     ASLLPGLGAEGLVLGAKLAVSCLLILPQSVLLGATFPLMSAGLVRAHPAASGESVAML
                     YFTNSLGAAAGVLASGFLLIEWFGLPGTLRTAGAINLALAAAVWLLARPMRAPAIAPA
                     AGGAAHPLRLLLAVAFLTGLASFIYEIGWIRMLSLVLGSSTHSFELMLSTFILGLALG
                     GLAVRRRVDASDAPERLLGWVQVVMGLLALATLLVYDRSFELMEYLMQGLARNDAGYF
                     LFNLSGQAIAALVMLPATFCAGMTLPLITGALLRRGAGEGAIGQVYAANTLGAIAGVL
                     IAVHVGLPQFGLKGALLLGSLVDAGLGLYLLYRYAPVRSAFAPAALACAALFLPVAAW
                     FDLDVNKMTAGVFRHGNLDSSRDAKILYARDGKTATVHLVQYGSATSIRTNGKSDGSV
                     NLTGEAEGDRGTDEITMVLTGALPLALKPEAKNAAVIGIGTGLTTHTLLQGLLLERVE
                     TVEIESAMAEAARGFMPRNASAYADPRSAIHIDDAKTYFSSRSRRYDIVISEPSNPWV
                     SGVSSLFTREFYRRIRGHLVPGGVLVQWFQLYEIDTSLLASVMRALGEEFPHYAVYAP
                     TDHDLLIVAGETAPPAPQERIFDQPGLAKALRTVHLHAIGDLDARYLGNRATLEPLFA
                     SYGMPANSDYYPVLDLNAARHRFTEKSATDVVALLNLGVPVLEMLEPRYARRPVSPMH
                     KGAYAFERVENARQAAYARDFLLRDLPPEPVGIPTQLQKDLEVVKLRLFDCRNPRELD
                     VWLHSLLRVARLVNPLLPAAEAQSLWGAAPGAACVRTLRGFQRQWLALFRAVGARDGA
                     AMAELGAALLSATPELHADAREYLLMAGMTGAIASQQRELARRLWEEQQPQLSRLLAQ
                     PVFRLLRCHAGAGDPAACAAAFRSYAED"
     gene            25921..>26993
                     /locus_tag="VFB53_02435"
     CDS             25921..>26993
                     /locus_tag="VFB53_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85619.1"
                     /translation="MTPALRLSAYFCAYFLYAGALVPYFALYLAARGYGAGQIAVVLA
                     MPQLARVAAPAFWGWLADRSGAARGIVVFSAAVLLAGFGLMHWVEGYAGVVAIMLGMS
                     LLAAGALPLVEALTLGSLEGRTEGYGPIRVWGSVGFIVGVLATGAWLDAHPPQSLLGV
                     IVLLAALSLAVSFLLPAGRAAAAGGPAEANLAAVLLRPEVLAFFAACFCMNLSHGALY
                     AFYSLYLDAEGYSKTAIGVLWTLGVVAEIALFLVLPRLLRRHSLRAVLGASCACAALR
                     FVLIGWGVHSPLLLALAQLLHAATFGAYHASSVAIVHRLFTGRLAARGQTLHSSVSYG
                     LGGAAGTLLAGWSWVALGPALSFT"
BASE COUNT         3991 a         9183 c         9849 g         3970 t
ORIGIN      
        1 ccgaccccaa gctgccggtc gagcaggagg ccgcctccgg cacgctgatt actttcgagg
       61 aagacccgca catggaaaaa gcgatcgtct ccggcgtcgc cgcgcagcgc gacgaggcca
      121 aagtcaccgt ccacggcgtg cccgacaagc ccggcatcgc ctacgcgatc ctcgggccga
      181 tcgccgacgc cggcatcaac gtcgacgtca tcgtgcagaa cgtcagccac gacggcatga
      241 ccgacctgtc cttcacgctg aaccgcggcg acctgaagcg cgccatgaaa atgctcaacg
      301 acaaggtcaa gccggccatc aagtgccgcg agatcacggg cgacgaccac atggcgaagg
      361 tttcagtggt gggcctgggc atgcgctcgc acgcgggtat cgcggcgaag atgttccgca
      421 cgctggccga ggagggcatc aacatccaga tgatctcgac ctcggagatc aagatctcgg
      481 tggtggtgga cgagaagtac gccgagctcg ccgtgcgcgt gctgcacaag gctttcgagc
      541 tggatcaagg gtaaaatccc cgccagccgg agtgatgccc gagtggccga agggggcgcc
      601 ctgctaaggc gttatacggg caaaacccgt atcgagggtt cgaatccctc tcactccgcc
      661 agtctcttcc cctgcctgac cagctcctcc agcacctcgt acggctgcgc gccgacgacg
      721 ccctggttgc cgacgacgaa ggttggaacg ccagtcacgc cgtagcggcg cgacttctcc
      781 caatccgcat cgaccgccgc ctggtgcgtg cgcttcgcga tgacctcacg agcctggtcc
      841 ggcggcaagc cgacggaccc cgcgatgtcg accagcactt cggcgtcgcc gatattgcgc
      901 gcttcgacga agtacgcgcg gaacatcgcg tggtggatcg cctctccccc gggctgcgtg
      961 tccgcccagg cggcgagttc ctgtgccaga cgactgttgt aggtcatcgt gcgcgtgccg
     1021 tagggcaggc cttcggcttc catgcgcgcg cgcatctgcg cctgcatctt cgggatgtcg
     1081 tagccgcggc cggcgaacag gtcctcgagg ctgcggcctg cggcgggcgt ctcgggatgc
     1141 agcgggaagt ggatccactt cacgcgcacg ccgtgttcct tcctgagtcg ctcaatacgc
     1201 acggtactga gatagcacca gggtcagacg aagtcggaaa agatttccag ggtcagcggt
     1261 tcggtggcca tgggcgccat tatgagggat cgggctgcgt ggcgcgccaa agcccggcta
     1321 cactcggcgc acagcgacca aggagcccgc ccgatgctca agacttacgc cacgctggcc
     1381 ctgaaggacg aaggcccgca cgttctcgtc gtcaccctca accgccccga ggtgctgaac
     1441 gccatcagca cgcagatggg gcgagactgg ctcgacctgt tcaacggcct ggccgccgat
     1501 cccggcgaca cgcgctgcat cgtgctcacc ggggccggcg agcgcgcctt ctgcgccggc
     1561 gcggacttga aggagcgcga cggcatggcg gtggagacct ggcgcacgca gcacgcgcag
     1621 ttcgagcagg ctttcgtcgc cctgctggag tgcccggtgc cgatgatcgc cgcggtgaac
     1681 ggccatgcct acggcggcgg cctggagacc gcgctgtgct gcgacttcat ctatgccgcg
     1741 aaaagcgcga agctcgccct gtcggaactg cgcctcggca tcatgccggg cggctgcggc
     1801 acgcagaacc tgccgcgcgc tgtgggcgag cgccgcgcca aggaactgat cctgaccgcc
     1861 cgcgccttca cggcggagga gggcgccgcg tggggactgg tcaacagggt ctgcgagccc
     1921 ggccggacgc tggccgacgc gctggagacc gcgcagcaga tcgcggcaaa cgcgccgctc
     1981 gcggtgcgcc aggcgaagaa gtccatccat tacggcttgc agatggacct cctctccggc
     2041 taccgcttcg agatcgaggc ctacaaccgc ctggtcgaca ccgaggaccg cagggaaggc
     2101 gtgcgcgcct tcaacgagaa gcgcaagccc tccttcaaag gccgttaagc ggccttttgc
     2161 gtccagcgct cgagcgcctc ccagccgccg atcagctcgc cgttgatgaa cacctgtggc
     2221 acggtctgcg cgccggcgat ggcgcccacc acccgggtgc gcagcttgtg gtcgagcgca
     2281 atctcggcgt agtcgtagcc gcgctcggtg agcaaggcct tggccttggc gcagaagctg
     2341 cagccctcgc gcgtcaggat cgccacctgg tcgggcttct tcgccttcgg gttgatccag
     2401 gcgagcatgg tgtcggcgtc cgacacctcg aacgggtcgc cggctttctg cggctcgatg
     2461 aacatcttcc gcacgacgcc gtcctgcacc agcatcgagt agcgccagga gcgcttgccg
     2521 aagccgagct cggccttgtc caccagcatg cccatcttct cggtgaactc gccgttgccg
     2581 tcggggacca ggcgcacgtt ggcggcttcc tgttcctttg cccactcgct catcacgaag
     2641 ccgtcgttca ccgaaatgca gacgatctcg tccacgccgt tggcgcggaa cgccggcgcg
     2701 agctcgttat agcgcggcag gtgggtggag gagcaggtcg gcgtgaacgc gcccggcagc
     2761 gaaaacacca ccaccgtgcg gctcttgaaa atctcgtccg tggtgaccgt cttccattcg
     2821 ccgttgctgc gcgtgcggaa cgagacctgc ggtaccctct gtccttcgcg attcgtaaac
     2881 atctatcgac tcctgaattg tgattgagga aagcttggac tctagcgatc gccagccgtc
     2941 cgcgccaatc gtttgtcggc atgcgcctca tagccgcggg ctaacgcgtg gctgatctag
     3001 aatcgtcggc gtggcgaagc cggtggaggt gaaccagttc aagctcggca tgcgcagcct
     3061 ggcgcacgcg gtgaacatcg tcaccgccgc gcacggcggg caccgctgcg gcatgaccgc
     3121 caccgcggtg tgctcggcca ccgcggagcc gcccaccgtg ctggtctgcc tcaacagggc
     3181 ctccgcgacc tacgccggcg tgacgaagag caagtcgttc tgcgtcaacg tgctgcgcgc
     3241 cgaggactcg gacctgtcaa atttgttcag cggcgcccag tcgggcgagg ggcgattcaa
     3301 gtcgcgcgaa tggaccaggc tggcgaccgg ctcgccggtg ctgctcgatg ccctggtgtc
     3361 cttcgactgc cgcgtgatca agaagctggc gcacggctcg cacaccgtgt tcctcggcca
     3421 ggtggagcag gtgctgttcg gcaagaaggg ccggccgctg ctctacgccg aagggcagta
     3481 ctcgaagctc gcctcgctgg cgcacggcga gccgctgccc gagggcctgg actactgggg
     3541 attctagaga cgcttccggt ataatcctcc tcctcgcgcc cgtagctcag ttggatagag
     3601 tacttggcta cgaaccaagg ggtcgtgggt tcaaatcctg ccgggcgcgc caatcaatcg
     3661 gggggcttag cgtgagctag gccccttcgt tttcatgccg cgccatcgca tccgacgcat
     3721 cgtccgctcg gccttgcgca agctgcgcag cgcgcccccc ggcctggtgg ccctcggcgc
     3781 gctgctcgcc ctcgctgccc tctggctcgc ggtcaacggc ctggtgcagg tggtgcgcaa
     3841 gccctccgag ctgttcttcc cggtgagcgg ggtgctgcac aagtcgccga ccgagacctg
     3901 gcggcactac gaaccgatgt tccgcgcgca tgccaccgcc gtcgtcacag ccgacctgct
     3961 cgccgcgctg gcgcaggtgg agagcgccgg caatccggtg gcgcgcacca actggcgctg
     4021 gcggttgacc gaagatccct tcgacgtgta ccggccggcc tcgagcgcgg tcggcatgtt
     4081 ccagatcacc gacggcacct tccgccaggc gcggcgctat tgcatccatc accacgcggt
     4141 ggtcgaggac ggcccctggc acgactggga ttcctgctgg ttcaacagct tttacttccg
     4201 cgtggtcccc agccatgccg cggagatgac cgcggcgttc ctcgaccgca gcgtggcgcg
     4261 cgtcctcgcg cgccagaagg tcgccaaggc gacgctgcgc cagaagcagg agctggccat
     4321 cgtgatgcac ctgtgcggcg ccggggcggg agaggtgtac gcgcggggcg gcctgcggct
     4381 cgccgcgaac cagcgctgcg gcgggcagga cgtgcacgcc tacgtcgagc gcgtgcgcgc
     4441 catgcaggct cagttcgcgc gcctggcgga gcgcgccgca gcgcccctgc atctggcgtc
     4501 gggcacgggc acgggttacc cttcgcgctt caaggaggca gcatgacatc cctcgcccgc
     4561 ggcctggtgg ccgcgtttgc agcgctctgg ctcgcctcgg cggccgcgca acaggcctac
     4621 ccgacgaaga cgatccgctt catcgtcacc ttcccgccgg gcggcagctc ggacctgatc
     4681 gcgcgcgcca tcgcgccggt gctctcggac cggctgcgcc agtccgtgat tgtcgagaac
     4741 cgcccgggcg ccggcggcaa catcggcatg gagatgctcg ccaagtcggc gcccgacggc
     4801 tacaccatgg gcctgggagc cgccggcgcg ctcgccgcga acgtcagcct gtatccgaag
     4861 ctgccctacg atccgcgcaa ggacttcgcg ccggtctcca acgtggcttt cgtgccgttc
     4921 ttcatcatcg cccatccctc gctgccggtg aacaacctcg aggagctgat cgcgctggcg
     4981 cgcgccaagc ccggcgagct cctgctgggc tatggcggca acggcacggc catgcacctc
     5041 tcgggcgagc tgttcaagct gatggcgaag gtgcagatgg tgaacgtgcc ctacaagggc
     5101 agcggcccgg cggcgcagga cacgctgggc gggcagttgc cgcttgccgt ggtcgacgtc
     5161 gcctcggccc tggcgcaggt gaaggccggg cggctcaagg cgctcgccgt caccagcgcc
     5221 aagcgcatcg gcgcagcgcc ccaggtgccc acggtggcgg aggctggcct gccgggctac
     5281 gacgccaccg gctggttcgg cgtggtgatg cccgcgggca cgccggccac ggtcatcggc
     5341 cgcatgaatg ccgagctggt cgcggcgcta aagcgcgcgg atatccgcga ccgcgtgatc
     5401 gccgccggcg cggagccttc gccgagcacc ccggccgagt tcggcgaact gatccgcacc
     5461 gagatcgaca agtgggcgga ggtggtcaag ctctcgggcg cgcgcgtcga ctgagcgtgg
     5521 cgcggcgcaa gattccgatc ctgccttcga gggtcacggt cgaaggcctg gatcgcgcgc
     5581 cgcaccgcgc cttcctgcgc agcctgggcg tgcacggcgc ggacctgggc aagcccttcg
     5641 tcggcgttgc ctccaccgat ggccgcgtca cgccctgcaa tgcgctgctc ggcgagttcg
     5701 cgcgcgaggc ctgcgcgggc gtgcgcgctg cgggcggggt gccgttcgac ttcgcctcga
     5761 tctcggtggc cgattcgatg tccatgaacc acgccggcat gcgctactcg ctggtgtcgc
     5821 gcgaattgat cgccgacggc gtggaggcgg tggtgcgcgg ccatgcctac gacgcgctgg
     5881 tcgccttcgc cggctgcgac aagaccctgc ccgggatgat gatggccatg gtccggctca
     5941 accttccggc ggtctttgta tatggtggcg ccgcgcttcc cggccgctgg cggggcaggg
     6001 acgtgacggt gctcgacacc tacgagggcg tgggcgcggt gatggcgggc gacctgccgc
     6061 aggcgcagct cgacgagctg gaagcggttt gcctgccgac actgggatcc tgcccggggc
     6121 agttcaccgc caataccatg gccatggtgt cggagaccct cggactcgcc cagccgggca
     6181 cggccacgct gcctgcggtc gcggaagggc gcagcgcagc cgcgcgcgaa gccggcgcgg
     6241 cagtgatgcg cctgctgcgc gagggtggtc cgctacccag ggaactggtg acccgcaaga
     6301 gcatcgagaa cgcctgcgcc gccgtggccg ccaccggggg ctcgaccaac gccgcgctgc
     6361 acctgccggc gatcgcgcac gaggccggca tccgcttcga tctcgacgat ttcgcccgca
     6421 tcgccaggcg aaccccgctg atcgcggaca tgaagccggg ggggcggtac ctcgccaagg
     6481 acctccacgc cgcggggggc gtctacagcg tgctgaagga gctgctgcgg cgcggtgcct
     6541 tgcacggcga ttgcctgacg ctctccggca ggactctgga gcaggcgctc gaaggacagg
     6601 gcggcgccga cggcgaggtc gtgcaccgcg aaccgctgtc gcccgacggc ggcctggtgg
     6661 tgctcaaggg aaacctgtgc cccgacggcg gattgatcaa ggtcgcggga ctcaagtccc
     6721 tggtgttcga tggccgggcg cgcgtgttcg acagcgaaga ggcctgcgcc gcgcaggtgc
     6781 gcgcgcgcaa gctgcgtccg ggcgacgtgc tggtgattcg ttacgaagga ccgcgcggcg
     6841 gtcccgggat gcgcgaaatg ctcggcgtca ccgcgctgat ctacggccag ggactgggcg
     6901 agaaggtggc gctggtgacc gacgggcgct tttccggcgc gacgcgcggc atgatggtcg
     6961 gctacgtctg ccccgaggcg gcggccggcg ggccgatcgc gctggtgcgc aatggcgatc
     7021 gcatcaccat cgatgcccgc aaggggcggg cgacgctgca ggtgtccaag gccgaactgg
     7081 cggtgcggcg ccgcgcctgg agagcgccgc ggcaggcgaa gctgtcgggc gccctggaga
     7141 agtacgcccg gctcgtcggc cccgcgaatc gcggggcggt gacccattcc ggaggtggaa
     7201 tttgagtgct gcagtgcatc tggggttcat cggtaccgga ctgatgggcc gcccgatggt
     7261 cctgcgcctg ctggccgcgg gctatccggt gtcggtctgg aaccggagcg cggccaagct
     7321 cgagccgctg ctgtcggaag gcgcgctggc gcagtcttcg ccggctgccg tggcacgtgc
     7381 cgcggacatc gtgatgatgt gcgtgaccga ccagggggcc gccggggagg tgctgttcgg
     7441 tcccgacggg gtcgccgcag gcggcgccgc cggcaagctg gtggtggatt tctccagcat
     7501 cgcgccggca tcggcgcgcg agtttgccgg gcgattggag cgcgaatgcg gcatgggcat
     7561 ggtggatgca ccggtttcgg gcggcacgcg cggagcggaa cagggcacgc tcgccatcat
     7621 ggcgggcggc aggcccgaac acatcgagcg cgtgcggccg gtgatcgccc atctcgcgca
     7681 gcgcttcacg cgcatgggcg aagccggcgc cggacaggtg accaagttgt gcaaccaggt
     7741 catcgtcggc agcctgctgc cggtgatcgc ggaggccgtg cggctggccg aggcggcggg
     7801 cgtggacgcc aactcgctgc cgggtgcatt gcaaggcggc tttgcggatt cgcttccgct
     7861 tcaggtattt ggcgcgcgca tggcggcgcg caacttcgat ccgccgctgg gatcggtggc
     7921 gatcatgctc aaggaccttg agaacgcggc gaccgtcgcg aaacagacgg gcgtgccgtt
     7981 gccgatggcg cattgcgctg cggaacttta tcgcctgctc gttgcgcagg gacgcgccga
     8041 gcatgaaccg agcgcgctgg tcgaactgct cgaaggtaag cgcgcgtaaa atttttcacg
     8101 gatgccgcgc gcgcattgtg ttcgcgctgc gatgtcatta atattcacgc tcgttcgcgc
     8161 aagggggaga gtgcgtggcc atgcgatccg tcaccaaagc cgatctggtc gaagcggccg
     8221 cgcgcgccgg caacctgagc aagtcgagcg ccggcgaagc ggtgaacgcg gtgttcgatg
     8281 caatcgtgtc gggcgtggcg aagggcaatc gcgtcaccgt ggtcggcttc ggcactttcc
     8341 tgccgcgcaa gagaaaggcg cgcgccgggc gcagtccgct gaacggcaag gagatccgca
     8401 tctcgtccaa gacggtgccg gcattcgccg ccgggcaggg cttcaaggac cgggtggaca
     8461 accgctaagc cggggccgca ttgcaaagcg cagggagcct tggcagaatc gctgcccatg
     8521 gaccacctgc tgattctcac tctcgacagc catggcgtgc cgcaccgctg ggtcagctgg
     8581 cagcaggcct gcttctacta cgccaagaac ctggtcgcct ggacgctggg cgagcgcacc
     8641 ttcacgttcc gcggcggcat ttcgcgcctg accggcgagc gctccagcat caccgcgcat
     8701 tcgatcatcg ccatccgcgg caaggcgctc gcggccaagg gtttcaacca ggtaccgccg
     8761 ctcaacaacc gggaattgtt ccggcgcgac cgccacacct gcgcctactg cggccatgag
     8821 tacggctatt tccggctgac gcgcgaccac atcacgccgc tgtcgcgcgg cgggcgcgac
     8881 acctggatga acgtggtgac cgcgtgccgg cactgcaacg gccacaagcg caaccggttg
     8941 cccgaggagt cgggcctgga gctgatgtac gcgccctacg tgccgaacaa ggcggagtac
     9001 ctgatcctca ccaaccgccg tatcctcgcc gaccagatgg agttcctgcg gcagcacgtg
     9061 gcggcgcaca gccgcctggt cctggcccct gcctgatttc tggcgcaaca gcggttacca
     9121 cctgctgcag aaggatgcgg cggggcgcct cgccgtcacc gacgatttcc tgcgcgcctt
     9181 ctacctgcgc cccgaagtgc acccggtgga ggagtcctgc gacgccgagc tgcgcctgca
     9241 tgccgggctg atggcggcgc cgcggcgcgc ggtcggcgcc gcggagatcg acgcggtcgc
     9301 ggacgaggat gcgcgccaca actaccgcgt gctgctcgcg tttcgcgacc ggctgctcgc
     9361 ggcgggcacg gtcgaaggct gctacatggg cctgttcaag ggcgcgatcg acgtgccgcc
     9421 gctattcatc gagcagctgg cgcacgtcat cgtgcgcaac ctgctgcagg attgcgacga
     9481 cccgctgcgg ctgcgcgccg ccgaactgtt cttccgcgag cagaaggcga ccctgcagga
     9541 cgggcacgtg ctgctcgccg acctggagac cgtggcgacg cacgcggcgg ggaaccgtta
     9601 cggcaacctg ggcaagctga tcgtggaggc gcagggcacg gtcggcacgg tggacctgga
     9661 cgtgctcgac cgcgcgaacg ccgcgctgta ctgggagcgc gagtcgcgcc acgacaccgt
     9721 gatcagtctc acctacggca ggccggcgct ggacgccctg tgccgcgtgc tcgaggcctg
     9781 ggtggcgcat ttccacggcc tgcaggtgag cgtgaagccg ctgcgccaca tcgaggaagg
     9841 acgctgggcc tggcacatcg gcctggatgc cgagtcgacg gagatcctga acgcgctgtg
     9901 ggcgggcggc caggtggatc cgggacgcct gcgcaacatc gtcgcgctgt tcggcctgca
     9961 attctccgac ccggcggcga tgcgccagga catcgccggg cgcaccgtgt acctggcgct
    10021 gtcggcgaac gccgatcagg aagtgcgcat gaagccgcag aacctgctca ccaatctgcc
    10081 gctgcatgaa gcctgagtac tgggaccggg cgaagagggc gcttgcgcgc cgcgacccgg
    10141 tgctcgccgc catcatgcgg cgccaccccc gggtgcacct ggtggcgcgc ggcgagccgt
    10201 tccagaccct ggcgcgcgcc atcgtcggcc agcagatctc ggtgaaggcg gcgcagtcgg
    10261 tgtggaaccg cgtgctcgag gtggcgccgg cgatgacccc cgccgcagtg ctcgcggtgc
    10321 ggcggccgcg cctgcgctcc tgcgggctgt cggaccgcaa gaccgaatac atcgccgacc
    10381 tcgcgcgcca cttcgccgaa gggacggtgc acccgcaccg ctggccgcag atggacgacg
    10441 aggcggtgat cgccgagctg gtggcggtgc gcggcatcgg ccgctggacc gcggagatgt
    10501 tcctgatgtt caacctgctg cgccccgacg tgtacccgct gggcgacctc gggctgctga
    10561 acgccctgcg caggagctac ttcaacgggc gcaaggcgcc gctgtcgcgc ctgcgcaagc
    10621 tgggccaggc ctggcagccc tggcgctcgg tggccacctg gtacctgtgg cggtcgctcg
    10681 acccggagcc cgtagaatac tgaccactgg tggccgcgac cccgctggct gccggaatcg
    10741 aggcgcccgc ggcgatgccc atccgattct ttcccgagga ccgcgaccag gcgctgaggg
    10801 tccggcgcta cctgatcgcc tcggggacca cgctgctggt ggtcctggtc tactgcgtgt
    10861 gctactgggc cggactcatg gcctggccgg tgctggcggc cggcatcgcg ttcgcggcag
    10921 tgcgcgccgt gctcttctat gccctgttcc gcaccggcgc caatcgccgg ttccgggacc
    10981 ccagcctcac gacggagcag atcgtcttcg ccacgttgaa cacggcgctg gtcatgttct
    11041 tcgccgagca ggcgcggccg gcgctgctgc cgatctacgt ggtgccgttc ctgttcggcg
    11101 tattccggct gcggaccagg gagttcctcg ggctcgggct ggtggtgctg ctgatcttcg
    11161 cggcgatgca gtatgtgtcc gcgcacctcg gtttcgcgcg ggtcgacgcc gcgcgcgaca
    11221 tggtcgactt cgtggtggtg gcggtggtgg tgccgtggtt cgcggtgttc ggcggctacg
    11281 tgaacgggct gcgcacggaa ctgggcggcg ccaaccgcaa gctcaaggcg gcgctggagc
    11341 gcatcgagaa gatcgcgatt cgcgacgagc tgaccggggt gtacaaccgc cactacctcg
    11401 ccgaacttct gcagcaggag aaagcccgct tcgaccgcgg cggcgcggtg ttctccgtct
    11461 gcatgctcga catcgaccat ttcaaggcgg tcaacgacgc gcacggccat ggcgccggcg
    11521 acgccgtgct gaggcagttc gccgagaccg tgcagtccgc gatgcgccgc gcggacgtct
    11581 tcgggcgcta cggcggcgag gaattcctgc tggtgatgcc gcagaccgcg ctcgacggcg
    11641 ccgccgcgtc ggccgagcgc atccgggccg cggtcgagag ctgcgcctgc gcggccctgc
    11701 cggcggggcg gcgcgtcacg gtcaccatcg gcgtggccac catcgcggcg ggcgaggaca
    11761 acgcggcgct gctggcccgt gccgacgagt gcctgtatcg gggcaaggcg gcgggccgca
    11821 atcgcgtcgt cgcggtgggc tagaatccgc cccgtgaaaa ccaccttcct cgacttcgag
    11881 cagccgatcg ccgagctcga gcagcgcatc gaggaattgc gcttcgtgca ggacgattcg
    11941 gcggtggaca tctcggagga gatccagcgg ctcaccaaga agagccaggc gctgaccaag
    12001 gagatctacg ccaagctcac gccctggcag gtggcgcagg ttgcgcggca cccgcagcgg
    12061 ccctataccc tggactacgt cggcatgctg ttcgccggct acgaggaact gcacggcgac
    12121 cgcacgttcg ccgacgacgc ctcgatcgtc ggcgggctgg cgcgtttcga cggcctgccc
    12181 tgcatggtga tcgggcacca gaagggccgc gacacgaagg agaagatcgc acgcaacttc
    12241 ggcatgccca agccggaggg ctaccgcaag gcgctgcgcc tgatgaagct ggccgagaag
    12301 ttttccctgc cggtgttcac tttcgtggac acgcccggcg cctatccggg catcgacgcc
    12361 gaggagcgcg ggcagtcgga ggccatcggc cgcaacctgt atgcgatggc ggcgctgcgc
    12421 accccgatca tcgcctcggt gatcggggag ggcggctcgg gcggcgcgct ggcgatcgcg
    12481 gtcggcgacg tgacgctgat gctgcagtac gcgacctatt cggtgatctc gcccgagggc
    12541 tgcgcctcga tcctgtggaa gagcgccgac aaggcgccgg aggcggccga gacgctcggc
    12601 atcaccgcca accggctcaa aaccctgggc ctggtggaca aggtggtcag cgaaccgctc
    12661 ggcggcgcgc accgcgaccc gcaggccgcg gggcaggcgc tgaaaaaggc gctggccgag
    12721 gcgctgcggc agctgcgcga gaaaaagccc aaagagctcg tcgaggaacg cctcgagcgt
    12781 ctcatgagct atggcaagta caaggaagcc gccgagcgtt gacgcggccg gcctggcgct
    12841 gcgcggcagg cgggtcgccg tcgggctgtc gggcggcgtc gactccgtcg tcctgctgca
    12901 cctgttgcgc gacgctgcgc cgcagctcgg attcgttctg agcgcggtgc acgtgcacca
    12961 cggcctgagt ccgaacgccg gggcctgggc ccgcttctgc aggaagctgt gccgggcatg
    13021 gggggtgccg ctcacggtgc gccgcgtgcg ggtgcggcgg gcaggccaag gcctggaggc
    13081 ggcggcgcgc gaagcgcgcc gcgccgtgtt tgccggcttg ccggcggagg tggtggccct
    13141 ggcgcaccag ctcgacgacc aggccgagac ggtgctgatg aacctgctgc gcggcaccgg
    13201 cctgcgcggc gcgagcgcca tgcccgccgc ggggcgcctt ggaagaaaga tcctgtggcg
    13261 cccgctgctg gcggtgccgc ggcgcgcgct cctcgcgcat gcccgcgcgc agcggctgca
    13321 gtgggtcgaa gacgagtcca acggggacga ggcgctgacg cgcaacttcc tgcgccggcg
    13381 cgtcggcccg ctgctcgagg cgcgctttcc gcgctggcgc gagagcctgg cgcgtgccgc
    13441 gcggcatttt gcgtccgccg aggcgtcggc cgagcgcgcc ttgcgcgcct tcctcggcgc
    13501 gcagggcctg cgctcgccca gcgcagccaa gctcgtcgag atgctgcggc agttgaccgg
    13561 gcggggcgcg cgcaccgcca tcgcgcacga tggcgcggtg ctgcgcgcgt accgtggccg
    13621 cgtgtcggtg gagcggcccg gcgcgccggc ggaggaggtg cgcctgcgcc cggcgcgcgg
    13681 gcgcggcatc gacgcggcgc ggctcgagcg cgggcgcgtc agcttgcggc tgcgcgcggg
    13741 cggcgagcgg ctgcagctcg atccgcggcg tccgcggcgc acgctgaaga acctgtttca
    13801 ggaggcgggc gtcgcgcctt ggcagcgcga gcggctgccg ctgctgtatt gcgatgacga
    13861 gctggtctgg gtgccgcagc tgggcatcgc cgccggctgg cacgccccgc gcggagcgcg
    13921 cggcctggtg cccgagtggc gcccggctgc agcgtaagcc attgttttta cgcgagtcgc
    13981 gggctgtcca tgctagaatc gcggccagac tttttcggca atccacggag attggggaat
    14041 ggcagtccag cgcactttgt ccatcatcaa gccggatgcg gtgaagaaga acgtcatcgg
    14101 caagatcatc gcgcgcttcg aaagcgcggg gctgcgcatc gccgccgcgc gcatgatgca
    14161 cttgtcgcgt gcggaagcgg agggcttcta cgccgtgcac cgcgagcggc cgttcttcaa
    14221 ggacctggtc gacttcatga tctccggccc ggtgctggtg caggtgctgg agggccgcga
    14281 cgcgatcgcg gtgaatcgcg agctgatggg cgccaccgat ccgcaaaagg ccgccaaggg
    14341 cacgatccgc gcggatttcg ccgactcgat cgacgccaac gcggtgcacg gctcggacgg
    14401 accggaaacg gcgcgcagcg agatcgcgta cttctttccg gcctgcgagg tcttcgcgct
    14461 ctgacatgaa gaccaacctt ctcggcctgg acgccgccgg cctcgccgcg tatttccaga
    14521 cgctgggcga gaagccgttt cgcgcgcgcc aggtaatgca gtgggtgcac cagcgccgcg
    14581 aaggcgattt cgcgcgcatg accgaactgg cgaaagcgat gcgcgccaag ctggcgcagg
    14641 atgcgacggt cgcggcgccg cagatcgtcg gcgaccatgt ggccgaggac ggcacgcgca
    14701 agtggctgct caaggtggac ggcgccaatg cggtggacgc cgtattcatt ccggagactt
    14761 cgcgcggcac gctgtgcgtg tcgagccagg cgggttgcac gctggattgc gccttctgcg
    14821 ccaccggcaa gcagggcttc agccgcaatc tgaccacggc ggaaatcgtg ggccagctgt
    14881 ggctcgccaa ccgcctgctc gggggcgagc ggccggtgac caacgtggtg ctgatgggca
    14941 tgggcgagcc gctgctcaat ctggaccagg tgatcccggc gctgcgcctg atgctcgacg
    15001 acaacgccta tggcctgtcg cggcggcgcg tgaccgtgtc cacttccggc gtggtgccgg
    15061 gcatggaccg cctgcgcgac gagtgcccgg tggcgctggc ggtgtcgctg cacgctccga
    15121 acgacgcctt gcgcgaccgc ctggtgccgg tcaaccgcaa gtacccgatc cgcgaactgc
    15181 tgcaggcctg ccgccgctac ctggaaaggg cgccgcgcga cttcatcacc ttcgagtacg
    15241 tgatgctgga cggcgtgaac gacagcgacg agcaggcgcg cgagctggcg gcgctcgccc
    15301 ggcgcgtcag ctgcaagttc aatctgatcc ccttcaatcc gtttccggcg accgagttcc
    15361 gccgttcgcc gccggagcgc atccggcgct tcgccgaaat cctgcagcgc gcggaggtca
    15421 cggtgaccac gcgcaagacc cgcggcgacg ccatcgatgc ggcctgcggc cagctggcgg
    15481 gcgaggtcac cgaccgcacg cggcgctcga aggtgctgcg cgtcgcggag ctgcgctcgt
    15541 gagccgcgcg ctgtggctgg tgctggcgct gtgcgcggcg ggctgcgcga cgcaggcgcc
    15601 cgaggggccc gcctccggcg ccggcgcgat cgtgggggat gccggcacgc cgcgcaaccg
    15661 cgcgcgcgtg cacaccgaac tcgccacctt gtactacgcc cgcggcaaca tgggcgtggc
    15721 gctggacgag gcgcgcgtcg ccgtggctgc cgacgccagc tacgccaccg cgcacggcgt
    15781 gctcgggctg gtgtacatgg agttgcggga aaacgacctc gcgcggcaga gcttcgagcg
    15841 ggccttgagc cacgcagcgg aagatcccga catcaaccac aactacggct ggttcctctg
    15901 ccagaccggc aaggagggcg aggcgcagcc ctatttccgg cgcgcgctcg ccaacccgct
    15961 gtacgcgacc ccggcgcgca gctacgcggc ggcgggcacc tgtgccttgc gcatcggccg
    16021 gctcgccgaa gccgacgaca acctggggcg cgcactgacc atcgacccga gccagccgac
    16081 ggcgctgctg cagtcggcgc tgctgcgcta ccgccagggg cgcttcgacg aggcgcgccg
    16141 cctgatggcg cgccaggcgc aggtggccga ggcgacgccg gagtcgctgt ggctggcgct
    16201 gcgcatcgag cgccgccgcg gcgcgcgcgc cgacgagctg tcctacgcca accggctgcg
    16261 gcgcatgttc ccggattcgc cgcagtacca ggccttgcgg cgcggcgagt atgactagcg
    16321 cgcaagccgg cgccgccgca ccggcgtccg acggcaggga accggtcggc agcgtgctgg
    16381 cgcgcgcgcg cgaggcggcc ggcctgagcc tggaagacgc ggcgcgccag ctgcgcttcg
    16441 cgccgcgcca gctcgaagcg ctcgagcgcg acgcgctgga ccggctgccc ggcggcgcgg
    16501 cggtgcgcgg catggtgcgc agctacgcac gcctgttgcg catcgatgcg gagccgctgg
    16561 tggcgcagct cgccgccgac gccagggcgc aggacgcgga acgcctggcg cagcgcttcc
    16621 gccagccggt gccgttctcc gacggcagcc ggcgctccaa cctggcctac atcgcgttgt
    16681 ctctgacgct gctggccgtg gtcgcggcgg tggccttcga atggcgtcag gagcgcggca
    16741 ccgagcgcct gacattcgtg tccgctgcgc agcagcccgc agagccggtg cgggtggcgg
    16801 cggcgagcgc tgcgacgccc cctgcgccgg cgccgacggc gccgccggag gcggcccggc
    16861 cggtgcgcgc gccggtggcg gctgccgagg acggcgacgg agaagcgggc cgcggccgca
    16921 tcgtgctgca gttcgagcgg gagtcatggg tggagattcg cagcggcagc ggcaagctgc
    16981 tggtgtcgca gctgcacgcc cccggcgccg atcgggtggt gtccggcgtg ccgccgttcg
    17041 cggtagtgat cggcaatgcg cagcacgtgc gcctctccta caacgacaag cccgtggacc
    17101 tgatgccgca cgtcaaggtc gaagtcgcgc gcttcacgct ggaatagcga tgggcgcgcg
    17161 ccggccgtcg agccgcgtca tggtggggcc ggtggccatc ggcgggggcg cgccggtggt
    17221 ggtgcagtcg atgaccaaca ccgacaccgc ggatgccgag gccaccgcgc gccaggtcgc
    17281 cgagctgtgg cgtgccggtt cggagctggt gcgcatcacg gtgaattcgc ccgaagcggc
    17341 ggcgcaggtc gccggcatcc gcgcgcgcct cgacgccatg ggctgcgacg tgccgctgat
    17401 cggcgatttc cacttcaacg gccacaagct gctgacgcag ttcccggatt gcgcctgggc
    17461 gctggcgaaa taccgcatca accccggcaa cgtcggcaag ggggcgaagc gcgacgagca
    17521 gttcgcgacc atgatcgagg ccgccctgcg ccacggcaag ccggtgcgca tcggcgtcaa
    17581 ctggggcagc ctcgaccagg cgctgctggc gcgcctgatg gacgagaacg cgagccgcgc
    17641 cgagccgctg gacgccgccg aggtgatgcg cgaggcggtg atcctctctg cgctggaatc
    17701 ggcgcgccag gccgaggcct ggggcctgcc ggccgaacgc atcgtcatct cctgcaagat
    17761 gagcggcgtg caggacttga tcggcgtgta ccgcgaattg gcgcgccgct gcggctatgc
    17821 gctgcatctc ggcctcaccg aggcgggcat gggttccaag ggcatcgtcg cctccagcgc
    17881 ggccctggcg gtgcttctcc aggaaggcat cggcgacacg atccgcattt cgctcacgcc
    17941 cgagccgggc ggcgaccgca cgcgcgaagt ggtggtggcg caggagatcc tgcagaccct
    18001 cggcatccgc tcgttcacgc cgatggtcgc cgcctgcccg ggctgcggtc gcaccaccag
    18061 cacgctgttc caggaactcg ccgcccgcat ccagtcctac ctgcgcgcgc agatgccggt
    18121 gtggcgcgaa cgctaccccg gcgtggagga catgcgggtc gcggtgatgg gctgcgtggt
    18181 caacggaccc ggcgaatcca agcatgccga tatcggcatc agcctgccgg ggaccaacga
    18241 gctgccggtg gcgccggtgt acgtggacgg cgcgaagacg gtgacgctca agggcgggcg
    18301 catcgccgag gaattccagg cgctggtcga ggagtacgtc aggaagaact acgcccccgg
    18361 cgccgcgcgc gcgcgcccca ggcacatccc cgtcaaggcg gcctgagccg tgagcgagcc
    18421 gatccgggcc gtgcgcggca tgaacgacgt cttgcccggc gacgccgagc tctggctgtg
    18481 gttcgaaggc cgggtggcgg ggtggttcgg gcgctacggt taccggcaga tccgcacccc
    18541 gctgctcgag cacacgggcc tgttccgccg cgccatcggc gaggtgaccg atatcgtcga
    18601 gaaggagatg tacaccttca ccgacgcgct caacgacgag agcctgacgc tgcgccctga
    18661 ggcgacggcc tcgacggtgc gcgcggcgat cgagcacaac ctgctgtacg aggggcccag
    18721 gcgcctgtgg taccaggggc cgatgtaccg ccacgagcgc ccgcaaaagg gccgctaccg
    18781 ggagttccac cagttcggcg ccgaagcgct cggctacgcc gggcccgacg tggacgccga
    18841 gctgatcgtg atgtgcgccg cgttgtggcg cgagcttggg ttgcagggca ttcgcctcga
    18901 gctcaacacc atcggctccg ccgcggagcg cgcgcgctac cgcgccgacc tggtgcgcca
    18961 cttcgaggcc catgcaccgg cgctcgacgc cgacgcgcag cggcgcctgc acagcaatcc
    19021 gctgcgcatc ctggactcga agaatccgcg agtgcaggaa gtggcggcgc aggcccccga
    19081 gctggtgaaa tacctgggcg atgcgtcgct cgggcatttc gagggcctgc ggcgcctgct
    19141 ggcggcgagc ggcgtggact acaaggtcaa tccgcggctg gtgcgcggcc tggattacta
    19201 caacctcacc gtgttcgagt ggatcaccga ccgcctcggg gcgcagggca ccgtgtgcgg
    19261 cggtgggcgc tacgacgggc tgtgcgcgca actcggcggc aagccggcgc cgggagttgg
    19321 cttcgccgcc ggcgtcgagc ggctgatcgc gctgttgcgc gacgcgggcc gcgcggcggc
    19381 ggcgcccccg ccgcatgcct atctggtgca ccatggggag ggcacgctgc cgtacgcgtt
    19441 cacgctggcc gaggagttgc gcgcggcggg gctcgccgtg gtgctgcatg ccggcgccgg
    19501 caatatcaag gcgcagatga agaaggccga tgcgagcggc gcgctgctgg cgctgatcgt
    19561 cggcgaagac gagctcaagg cggcggcggt gacggtgaag ccgttgcgcg atgcgcgccc
    19621 gcaggagcgc gccccccgcg atgcgctggc gggccgtctg caggaaatac tcaagggatc
    19681 ctaggacatg gcattgcaga acctcgatct ggaagaacag gagaagctcg aagagctcaa
    19741 ggcctggtgg cggcagtacg gcaacctgat cctgatggcg atcaccgccg tggcgatcgc
    19801 ggtcgcaggc tggggcgcct ggcgctggta ccagcgcaac gaggcgcagc aggcgagccg
    19861 gctctacgaa tcgctggcgg cggccgcccg cgccggcgat gccaaggcgc tgcgcgacgc
    19921 gggcggcgcc ctgaccgagg ggtatccgcg caccctgcac gcctcgatgg gggccctggc
    19981 gtcggcgcgc ttccacttcg accgcggcga gctgaagagc gccagggcgc agttgcaatg
    20041 ggtgatcgag cgctcccggt ccgaggacct gcgcgacctg gcgcggctgc ggctggcggc
    20101 gctgctgctc gacgagaagg aatacgacgc ggcgctcaag ctgctcgagg ccaagccgct
    20161 ggaggcctat gccgcccagt acgcggcatt gcagggggac gtgctggcgg cgaagaagca
    20221 gcccgcggag gcgcgtgccg cctaccagcg tgcgctggac aaggccggcg aaggcggcgc
    20281 tttccgcgac agcgtgcaga tgcgcctgga cgcgctcggc ggctgagcat gtcgcggcac
    20341 gcggcgctgg tcctgctgtt gccggcgctc gccggctgct tcggctcggc gccggccgga
    20401 ccgcagcccg ccgagctgcc cgcgctggag ccggcgcgcg agccgcgcca gctctggtcg
    20461 gccgcggtcg gcgccgcggg cgcgttcgcc ttccagccgg ccctcgccgg cgacagcatc
    20521 tacgccgcct cgcgcaacgg cgccgtcgcg cggctcgagg cggcaggcgg ccgggagcgc
    20581 tggcgcatcg cgctggatgc gaagctctcg ggcggcgtgg gtgcggacgc gcgcaccgtg
    20641 gtggtggcga gcgaccgggg cgaagtattc gcgctggagg ccgccgacgg caagctgcgc
    20701 tggcgagccc gcgtatcgag cgaagtcctg gcggcgccgc gcgtgcacgg cgacctggtg
    20761 ctggtacgca gcgccgacag ccgcgtgtac gcgatgggcg ccgaggacgg caagcggcgc
    20821 tgggcctatc agcgcgcgcc gtcggcactg atggtgcgca gcccggcggg catggtgccg
    20881 caggcggaca cgctgtacgt cggcttcgcg ggcggcaggc tggtggcgct ggcgctgtcc
    20941 aatggcggcc tgcgctggga ggcggctgtg gcgcagccca ggggcgccac cgaactcgaa
    21001 cgcgtggccg acgtggtggg cgacccggtg gtgatcggcc gcgaggtctg cgccgcggcg
    21061 ttccagggca gggtcgcgtg cttcgacgcg caaaacggca gccagctctg ggcgcgcgag
    21121 ctgtccagcg tgacgggcgt cagcgcggac gcgcgcaccg cctacgtcag cgacgaccgc
    21181 ggtgcggtgc acgcactgga ccgctccaac ggccgcagcc tgtggaaaca ggatcgcctc
    21241 gcgcaccgcc agctttcgct gcccgtgccg ctcggcgagc agatcgccgt cggcgacctg
    21301 cagggctacg tgcacctgct gtcgcgcgac accggcgcgt tcgtcgggcg cgccggcacc
    21361 gacggcagtc ccgtgcgcgc cgcgcccctg cctctgcccg aagggttcct ggtgcagacg
    21421 cagcgcggcg gcctgttcgc gctggcaccc taggcccggg cgccgcgtga agccggtcat
    21481 cgccctcgtc gggcgcccca acgtcggcaa atcgaccctg ttcaaccggc tcacgcgccg
    21541 ccgcgacgcg atcgtcgtcg acgttcccgg cgtgacgcgc gaccgccact atggagaagg
    21601 tcggctgggc gagcggccgt tcatcgtcat cgacaccggc ggactggaac tgcgcgcgcg
    21661 cgaaggaatc tacgtcgaga tggcgcgcca ggccgagcag gcgatggccg aagccgacgc
    21721 catcgttttt ctcaccgacg gccgcgaggg gctgcacgac ggcgacgctg aaatcgcgcg
    21781 gcggctgcgc aagctcaagg cgcgcgtctg gctggtggtg aacaagaccg agggcatggc
    21841 gagcgacgcg gcggtggcgg aattccacgc gctcggcctg ggccagccgc agccgatctc
    21901 gtctgcgcac ggcgacggcg tcggcgacct gatcgacggc gtgctggcgg aattcccgcg
    21961 tgccgacgag gaggcgcagg aggcgcgcga caagcacccg cgcgtggcga tcgtcgggcg
    22021 gccgaatgtc ggcaagtcca cgctggtgaa tgcgctggtg ggggaggagc gcgtgatcgc
    22081 cttcggcgag cccggcacca cgcgcgacgc gatcgaggtg ccgttcgagc gcgccggccg
    22141 gcactacacg ctgatcgata ccgcaggcct gcgcaagcgc ggcaagcagg gcgaggcgac
    22201 cgaaaaattc tccatcgtga agacgctgca ggcgatcgag cagtccaacg tcgccgtgct
    22261 ggtgctcgac gcggccgaag gcgtgtccga gcaggacgcg cacgtggccg gctacatcct
    22321 ggagcgcggc cgcgcgctgg tggtcgcggt gaacaagtgg gacgcgacgc aaagggaggc
    22381 gcgcgatcgc atcaagcagg agctcgcctg gaagctggtg ttcctcgggt tcgccgagac
    22441 gcactacctg tcggcgaaga gcggcaaggg cctgggagag ctgatgcgct cggtggacgg
    22501 cgcctacgcc gcggcgatgg ccaagctgtc gacgccgaag ctcacgcgcg cgctgatcgc
    22561 cgcggtcgag cggcagccgc cgccgcgcaa ggggttgcgg cgcccgaagc tgcgctacgc
    22621 gcaccagggc gggcagaacc cgccgcgcat catcatccac ggcaattcgc tcgacgcgct
    22681 gcccgacagc taccggcgct acctggaagg ggtattccgc gacggcttcg ccctggtcgg
    22741 cacgccgatg cgggtggaat tccgcaccgg ccgcaatccc tacgcggggc gcggcccggc
    22801 gccgcagcac cgcaagctgt cggaaaagcg cggcgggcgc tgacgcgcgc cggcccggcg
    22861 cgcgtcagtc ttccgcgtag ctccggaatg ccgcggcgca tgccgccggg tccccggcgc
    22921 cggcatggca gcgcagcagg cggaacacgg gctgtgcgag gaggcgcgac agctggggct
    22981 gctgttcctc ccagagccgg cgcgcgagtt cgcgctgctg cgatgcgatg gcgccggtca
    23041 tgcccgccat gagcaggtat tcgcgcgcat ccgcatgcag ttccggcgtg gcggagagga
    23101 gcgccgcgcc gagctccgcc atcgcggcgc catcgcgcgc accgaccgcg cggaacagtg
    23161 cgagccactg gcgctggaag ccgcgcaagg tgcgcacgca ggccgcgccc ggggcggcgc
    23221 cccagagcga ttgcgcctcc gcggccggca gcagggggtt caccagccgc gcgacgcgca
    23281 gcaggctgtg cagccagacg tcgagctcgc gcggattgcg gcaatcgaac agccgcagct
    23341 tcaccacctc gaggtccttc tgcagctggg tcggaatgcc cacgggttcc ggcggcaggt
    23401 cgcgcagcag gaaatcgcgc gcgtaggcgg cctggcgcgc attctctacg cgctcgaagg
    23461 cgtacgcgcc cttgtgcatc gggctgacgg gccgtcgcgc atagcgcggc tcgagcatct
    23521 ccagcaccgg cacgcccagg ttgaggagcg ccacgacgtc ggtggcgctc ttttccgtga
    23581 agcggtgcct ggcggcgttg aggtcgagca ccgggtagta gtcggaattg gcgggcatgc
    23641 cgtagctggc gaacaagggc tcgagcgtcg cccggttgcc caggtagcgg gcgtccaggt
    23701 cgccgatggc gtgcaggtgg acggtgcgca gcgccttggc cagcccgggc tggtcgaaga
    23761 tgcgttcctg gggcgcggga ggcgcggtct cgcccgcgac gatcagcagg tcgtgatcgg
    23821 tgggggcgta cacggcgtag tgcggaaact cctcgccgag tgcgcgcatc accgaagcga
    23881 gcagcgacgt gtcgatctcg tagagctgga accactgcac cagcactccg ccggggacca
    23941 gatggccgcg gatgcgccgg tagaactcgc gcgtgaacag gctcgagacg ccgctcaccc
    24001 aggggttgga cggctcggag atgacgatgt cgtaacgccg gctgcgcgac gaaaagtagg
    24061 ttttcgcgtc gtcgatgtga atcgcgctgc gcgggtcggc ataggccgac gcgttgcgcg
    24121 gcatgaagcc gcgcgccgcc tccgccatcg ccgattcgat ctcgaccgtc tccacccgct
    24181 ccagcagcag gccctgcagc agggtatggg tggtcagtcc ggtgccgatg ccgatcaccg
    24241 cggcgttctt cgcctcgggt ttgagggcga ggggcagggc gccggtcagc accatggtga
    24301 tctcgtcggt gccgcggtcg ccttccgcct ccccggtgag attgaccgag ccgtcggatt
    24361 tgccgttggt gcggatgctg gtcgccgagc cgtactgcac caggtgcacg gtcgcggtct
    24421 tgccgtcgcg ggcgtagagg atcttcgcgt cgcgcgagct gtccaggttg ccgtgccgga
    24481 acacgcccgc ggtcatcttg ttgacgtcca gatcgaacca cgccgccacg ggcaggaaca
    24541 gcgcggcgca ggcgagggcg gccggcgcga aggcggagcg gaccggggcg tagcggtaca
    24601 gcagatacag cccgagcccc gcgtccacca ggctgccgag gaggagggcg cccttcaggc
    24661 cgaactgcgg caatccgaca tgcaccgcga tcagtacgcc ggctatggcg ccgagcgtgt
    24721 tggcggcata gacttggccg atggcgcctt cgccggcgcc ccggcgcagc agcgcgcccg
    24781 tgatgagcgg gagcgtcatg ccggcgcaga aggtggccgg cagcatcacc agagcggcga
    24841 tcgcctgccc ggagaggttg aacaggaaat agcccgcgtc gttgcgcgcg agcccctgca
    24901 tgaggtattc catcagctcg aagctgcggt cgtagaccag cagcgtcgcc agcgccagca
    24961 gccccatcac cacctgtacc cagccgagca accgctccgg cgcgtcgctc gcgtcgacgc
    25021 gccgccgcac ggcaagcccg ccgagcgcca ggcccaggat gaaggtcgac agcatcagct
    25081 cgaacgaatg cgtggacgag ccgagcacca gcgagagcat gcggatccag ccgatctcgt
    25141 agatgaacga agccagcccg gtgaggaatg cgaccgcgag cagcagccgc agcggatgcg
    25201 ctgcgccgcc ggccgccggg gcgatcgcgg gcgcgcgcat gggccgggcc agcagccaca
    25261 cggcggccgc caacgcgagg ttgatcgcgc ctgctgtgcg cagggtgccg ggaaggccga
    25321 accactcgat cagcaggaag ccgctggcga gcacgcccgc cgcggccccc aggctgttgg
    25381 tgaagtacag catcgccacc gactcgcccg aagcggcagg atgcgcgcgc accaggccgg
    25441 cgctcatcag ggggaaggtg gcgccgagga ggacggattg gggaaggatc agcaggcagg
    25501 agacggcgag cttggcgccg agcaccaggc cttcggctcc cagccccggc agcaggctgg
    25561 cgtaggacca gtcggtgacg gcgatgaaca cttcgtgcag cccgagcgcg cacagcccga
    25621 ccaccgcctc gaccaaggca tagccggcca gggggttgcg cagccgcgtg gacacgcggc
    25681 cgcagaccgc ggcgcccaga gccatgccgc ccatgaacac caccagcacc agcgattgcg
    25741 cataggccgc gtgcccgagg aacagcttca ggtaatgcgt ccagatggat tcgtagatga
    25801 gcccggcgaa tcccgagacg gtgaacagca ggtagaaagc ggtgcgcgag ggcacgaagc
    25861 gcggcatggc gcgcgtttat agcacggtgt tccggcggcg tctgggtaag atgcgccgca
    25921 atgaccccgg cgctgcgcct gtccgcgtat ttctgcgcct acttcctgta cgccggcgcg
    25981 ctggtgccgt atttcgcgct gtacctggcg gcgcgcggct acggcgccgg ccagatcgcg
    26041 gtggtgctgg cgatgccgca gctggcgcgc gtggcggcac cggcgttctg gggctggctg
    26101 gccgatcgca gcggcgcggc gcgcggcatc gtggtgtttt ccgccgccgt gctgctggcc
    26161 ggcttcggcc tgatgcactg ggtcgagggc tacgcgggcg tggtggcgat catgctgggg
    26221 atgagcctgc tcgcggccgg ggcgctgccc ctggtcgagg cactcaccct cgggtcgctg
    26281 gaagggcgca ccgaaggata cggcccgatc cgggtgtggg gctcggtcgg cttcatcgtc
    26341 ggcgtgctcg cgacgggcgc ctggctggac gcgcacccgc cgcaatccct gctgggtgtc
    26401 atcgtgctgc tcgccgcgct ctccctcgcc gtgtcctttc tcctgcctgc gggccgtgcg
    26461 gcggccgccg gcgggccggc cgaagcgaac ctggcggcgg tgctgctgcg gcccgaggtg
    26521 ctggcgttct ttgccgcgtg cttctgcatg aacctctcgc acggcgcgct gtacgccttc
    26581 tattcgctct acctggatgc cgaggggtac agcaagaccg ccatcggtgt gttgtggacg
    26641 ctcggcgtgg tggcggaaat cgcgctgttc ctggtgctgc cgcgcctgct gcggcgccat
    26701 agcctgcgcg cggtgctggg tgcgagctgc gcctgcgctg cgttgcgctt cgtgctcatc
    26761 ggctggggcg tgcattcgcc gctgctactg gcgctcgcgc agctgctgca tgcggcgacc
    26821 ttcggcgcct accacgcctc gtcggtggcg atcgtgcacc gcctgttcac gggcaggctg
    26881 gccgcgcgcg ggcagacgct gcattcgagc gtgtcctacg gcctgggcgg cgcggccggc
    26941 acgctgctcg cgggatggag ctgggtcgcg ctcgggcccg cgctcagttt cac
//
LOCUS       DASRIE010000053        24933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1360175, whole genome shotgun sequence.
ACCESSION   DASRIE010000053 DASRIE010000000
VERSION     DASRIE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360175"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFB53_02440"
     CDS             complement(<1..875)
                     /locus_tag="VFB53_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZN85620.1"
                     /translation="METQRQRGALSQIQGRFETEARDPFDDGWAREDEALQPLETSVT
                     IERARTIISKNDSPDVPFDQSINPYRGCEHGCIYCYARPSHAYLELSPGLDFETRLFA
                     KTNAAELLKDELAKPGYVVSPISLGANTDCYQPVERKHRITRQIVEVLSACEHPLTIV
                     TKSALVERDLDILAPMARKNLVRVYVSIGTRDRELARKLEPRAASPQRRLDVVRNLNG
                     SGVPCGVMVAALVPALNDKTMEHVLEAAAQAGAAEAAYVIMRLPNELKDLFREWLAEH
                     YPQRAEHVMSVIRDMR"
     gene            908..2083
                     /locus_tag="VFB53_02445"
     CDS             908..2083
                     /locus_tag="VFB53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85621.1"
                     /translation="MNSTRRNVGLLAACQALLLSNNATLIAINGLAGFALAPNAALAT
                     LPVTCWVVGGAIATMPASLHMKRVGRQRGLTLGMAWGIAGALLCAAAVWQQGFWLLCF
                     GTLVFGVFNAYGQYYRFVAADVAGPGFKSTAISLVLAGGLVGGILGPGLSRATVDLLE
                     PRYSGAYLGLIAFSALSMLLLRFIRVPAPDARVQAAAGRPLAEIAAQPKFIVAVLSGA
                     ISYGVMNFLMTSTPIAMGICGHPYGDAAFVISSHVIAMFAPSFVTGSLIKRFGVLQVM
                     FTGALLNFAAIGVALSGIAVAQFWWSLVLLGVGWNFLYVGGTALLTETYRPEEMAKAQ
                     GANEQAIFVMMAISSFTSGLTVTAAGWERVNLYALPMVAAVALAILWYGITQRSRAA"
     gene            2080..3189
                     /locus_tag="VFB53_02450"
     CDS             2080..3189
                     /locus_tag="VFB53_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HZN85622.1"
                     /translation="MNAPAQVGQRLEEVDTPALILELDAFEANLRTVADLARGRVRVR
                     AHAKTSKCPEVGRRQMALGAVGVCCQKVSEAEAMVAGGIPDVLVSNEVVGAAKLARLA
                     RLGARAKIGVCVDDAGNVRDLDAAARAAGATLDVYVELEVGMGRCGVEPGAPALALAR
                     AVAAAPNLRFAGLQAYNGRAQHVRSNAERRALIEKAAAAVRATRALLEANGIACPLVT
                     GAGSGTFMFEIEAGAWDEIQPGSYAFMDADYAKNEWAAPLPRFAHALFVLTTVMSRPR
                     PDRAIVDAGLKASSVDSGMPAVWQRPGLAYALASDEHGFVEVAPGAPAPALGEKLLLV
                     PGHCDPTINLHDWYVCVRGGKVEALWPITARGALA"
     gene            3186..3620
                     /locus_tag="VFB53_02455"
     CDS             3186..3620
                     /locus_tag="VFB53_02455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN85623.1"
                     /translation="MKKRYLNPKELFDPRFFTHTVAVEGEFTLVYVAGQVSYDRDGVV
                     VGKGDMRAQAEQVFKSLTHNLKAAGAAWSDVVKLNGYMVRMSPEAVNQYREVRSRFLD
                     PKALPASTLIGVERLVHDDLLLEVEVVAAVAARPAAKSAAKR"
     gene            3622..4806
                     /locus_tag="VFB53_02460"
     CDS             3622..4806
                     /locus_tag="VFB53_02460"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85624.1"
                     /translation="MSRDRSGWVVVACAFTLMFVGFGVAYSFAAFFKAFQAEFAASRA
                     HVSLVFSLCALLWFSIGVPGGLLADRFGPRGVCLAGALSIAAGLAAAAHAHSLTVLYA
                     TYSIGIGVGIGLAYVPSVAAVQHWFTQHRVLAGGIAVSGIGAGNLLVPPLAAWWIELW
                     GWRGAYLGLMWLTLLAAVPAALLLGGRAATRAAAAGATLPGMSLAEAMRSRLFWLMYA
                     VAFLVCVGFFVPMVHLAPYAQDLGLSEAQGVSLVSLLGLGSLLGRFLMGGIAERLGHA
                     RSLVAIGAGLGLLFLVWWVARSYWLLAAFAVSFGTLYGGYVALAPTLSMELFGPRSLS
                     AILGALYTAAGLATLIGPTFAGASFDATGSYDASILGCAALSFAGAAIAFGLRAHFRP
                     AA"
     gene            4803..5570
                     /locus_tag="VFB53_02465"
     CDS             4803..5570
                     /locus_tag="VFB53_02465"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN85625.1"
                     /translation="MSRRPALAVSEERGVRTLHIGGEAIQSSMRLSDPYALELDYTRC
                     MMAFLLFHPRPARALKIGLGGGSLPKYFWRRLPSLRTRVVELDERVVAMARERFCLPP
                     DDARLRVDVGDGAEALAPECCDLLIVDAFADEHPPAALVSQAFFDAAWLALESPGALV
                     LNLMDDDPKLDERLQRVERAFGGAVLAFHALVDPNVIVIALKGAPPRLAWDLLRRRAR
                     ALEQRYGLPFTRMLARFRRMNRCDAEALIIAVQGDAA"
     gene            5567..6196
                     /locus_tag="VFB53_02470"
     CDS             5567..6196
                     /locus_tag="VFB53_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZN85626.1"
                     /translation="MKLESDSFIDQGAIPGRCAFAVPDAAAHVRLSDNRNPHFAWKNL
                     PAGTLSLALVCHDRDVPSRGDDVNQEGRQVPADLPRVDFFHWLLVDLKPEAGPVREGE
                     FSNGVTAGGKSGPAGARGTRHGVNDYTAWFAGDKDMAGDYYGYDGPCPPWNDTIVHHY
                     VFTLYALAVESCPVAGAFRGPELLKAIEAHVLGSAALTGLYSLNPAVKT"
     gene            6241..6942
                     /locus_tag="VFB53_02475"
     CDS             6241..6942
                     /locus_tag="VFB53_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN85627.1"
                     /translation="MAFAGALVFGLTGFGSALVTIPLATHFVPLQFALALFALADLAS
                     ALRVGLENPRSAVRAEWTRLVPMILVGTALGVTLLVNMPRAAGMLALGAFVIGYALYS
                     LLRRESPRIVAPGWAWVAGLAGGVTSTLFGAGGPPYAIYLSQRGLSKEQFRATMGFAT
                     MTSISLRVAAFLLTGLLLDPRVWLVAIAVVPAAQLGIVAAQRMFLRISREQLLRAVAL
                     VLLATGGTLVARAIG"
     gene            complement(6944..8149)
                     /locus_tag="VFB53_02480"
     CDS             complement(6944..8149)
                     /locus_tag="VFB53_02480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85628.1"
                     /translation="MLEARIEKMVGEFRAGASLPAAIQLWNGKRYDLGERPTVTLRVP
                     DAASLRYFVNADLAKLAEAYVEGHLEVDGPIADAMRVAEGLALKWGPARKARLPWLRS
                     AHSKERDAEAIHYHYDVSNDFYALWLDRRMVYSCAYFRSGAETIDEAQERKLDHICRK
                     LRLQPGDRFLDIGCGWGALVMRAAERYGASATGITLSQNQHDLANERIRAAGLQERCR
                     VLLQDYRDVPGEGVFDKIASIGMFEHVGLKNLPVYFGAIRRLLADGGIVLNHGITSVD
                     PDSRAVGMGGGEFIQKYVFPHGELPHLSLVLREMGAAGLEVMDAETLRLHYAKTLHRW
                     SDRLEARLEEAKAYAGEKRLRIWRTYLAGCAHAFERGWVSVQQVLAVRSSDARRNPLP
                     LTRDYMYGG"
     gene            complement(8161..9501)
                     /gene="gor"
                     /locus_tag="VFB53_02485"
     CDS             complement(8161..9501)
                     /gene="gor"
                     /locus_tag="VFB53_02485"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HZN85629.1"
                     /translation="MKFDFDLFTIGGGSGGVRASRFAASYGARVAVAEDGRFGGTCVN
                     VGCIPKKLFAYAAHFREDFEVARGFGWAVGEPRFDWATLLANKDREIARLNGVYEGAL
                     ARAGAQILRARARVIDAHTVEAGGARHTARHILVATGAWPQVPQIPGRELAITSNEAF
                     GLARLPRRALVVGGGYIAVEFASIFQGLGVETTLAYRGARLLRGFDAELGERLAEEMR
                     KKGVAIRLQANPAKLEPGIRVAYEDGAAEETDLVMFATGRKPNTAGLGLEGAGVRLGG
                     DGAVVVDAYSRSSVESIHAIGDVTNRLNLTPVATAEGMALAKTLFRGEPTATDHRNVP
                     TAVFSNPNLATVGLSEEQARARHGKVDIYKTSFRALKHVMGAHEERTFMKLVVDAATQ
                     RVLGAHMIGPDAGEIIQGIAIAVKLGATKSQFDDTIGIHPTAAEEFVTMRDKTT"
     gene            complement(9520..9786)
                     /locus_tag="VFB53_02490"
     CDS             complement(9520..9786)
                     /locus_tag="VFB53_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85630.1"
                     /translation="MSTHDKTLKLVTNLDRAGIEERLGQVRAAAQAASLADLAQMFAG
                     IEGAPRAQLEQKVRNAVKWLADKPEHKNLVAQLELVEINLPNLK"
     gene            complement(9783..11459)
                     /locus_tag="VFB53_02495"
     CDS             complement(9783..11459)
                     /locus_tag="VFB53_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN85631.1"
                     /translation="MAEKPVARPAATVLLLRDGSLGPEAFLLQRTKGAAFLAGAHVFP
                     GGALDKADEDPRVLRRVAGLSDAEASARLGMAGGGLAYWVAAVRECFEEAGILLAEGE
                     NGPVDAARAAQLAPYRPLLHGGKLGFAEFLEKERLLLRAGDLAYFGHWITAPGRARRF
                     DTRFFLALAPAGQAGSHDGAELVASQWLRPQEAIEREARGEMELVFATRNTLADLTRF
                     ARAKDALAHAREAEVETNRACWALGADGAAALYRRRDPQYFEIHWSDPDETGQTSVAL
                     EPGRPKRLDQYVTRLIAPNPGMMTGPGTNTYLVGEQELAVIDPGPAIDAHIAAVLAAG
                     AGRIRWVLCTHTHMDHSPAARAIKAATGAVLIGRPAPEQANQDRSFVPERVLAHGDRL
                     MMGGLTLRALHTPGHASNHLCYLLENTRMLFTGDHVMQGSTVVINPPDGDMRAYLASL
                     EMLLAEDILILAPGHGYLIGEPHREARRLIRHRLARETKVLNALSRLAAPTLEELVAT
                     VYDDVQPRLHPVAARSLSAHLGKLAAEGRVDAAAGRYTLVQSPDIGRSAR"
     gene            complement(11462..12337)
                     /locus_tag="VFB53_02500"
     CDS             complement(11462..12337)
                     /locus_tag="VFB53_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85632.1"
                     /translation="MFKDGLLKGKNILVTGGGTGLGREIAAQYLALGADLWIAGRRDG
                     VLQATARELMAQHGGKVRAHAVDIRDHAAVDAMAQRIWDESGPLSGLVNNAAGNFISP
                     TKDLTPNGFNAIANTVFHGSFYVTHAVGKRWIEGGHKGSVISILVTWVWTGSPYVVPS
                     AMSKAGLHVMTKSLAVEWGKHGIRLNAIAPGPFPTEGATRRLRPGASFGESTKVNPMR
                     RVGEMSELRNLATFLMAEGCEWLTGETIAIDGGGYLATGAYFTELDKMSDADWAKARE
                     LIKAQNDKDRAGRAA"
     gene            complement(12371..12538)
                     /locus_tag="VFB53_02505"
     CDS             complement(12371..12538)
                     /locus_tag="VFB53_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85633.1"
                     /translation="MELTLERYLQDEGLREELERRAHCERAEAMHRFFEQSTRALNLQ
                     RTPALRTDACG"
     gene            complement(12556..12783)
                     /locus_tag="VFB53_02510"
     CDS             complement(12556..12783)
                     /locus_tag="VFB53_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HZN85634.1"
                     /translation="MRIELQPGALRLARGQTLRVTGAQGSTICVSEGSVWITEENQPK
                     DVVLEAGGCYELRRPGLALVHAFGDASVALA"
     gene            12884..13783
                     /gene="gcvA"
                     /locus_tag="VFB53_02515"
     CDS             12884..13783
                     /gene="gcvA"
                     /locus_tag="VFB53_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HZN85635.1"
                     /translation="MRLPSLDLLRGFEAAARLLSFTRAGEELHLTQSAVSRQMQELEG
                     QLGVKLFERRHRALSLTDAGQQFYPAAAQVLATMRAATDRLRAQSGRGTLSVTTTHSL
                     AALWLIPRLAGFTKAHPGVDVRISADTRVQDLERDGLDVAIRHGPPQLAGEGATRLFG
                     ERVFPVCSPKLLKDAKKPLKVPADLRHHTLLQYDDPDARHPWLHWRTWLEVERLADLK
                     PAGRLSFSGYEQINAAAIAGHGVALGRSPLVKGSLASGELVAPFKRTADPARGYFAVV
                     SRNSAERPEVRDFVAWLLEEAKR"
     gene            complement(13773..14750)
                     /locus_tag="VFB53_02520"
     CDS             complement(13773..14750)
                     /locus_tag="VFB53_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZN85636.1"
                     /translation="MRAVEIEKPGGPEVLRPAERAKPDPRPHEILVKVAAAGVNRPDV
                     LQRMGLYAVPPDASDLPGLEIAGEVVACGSAVTMWKTGDAVCALTHGGGYAEYCVAPE
                     VQALPVPRGLSMVEAASLPETFFTVWSNVFDRGRLAPGETLLVQGGTSGIGVAAIQMA
                     SALGNRVFATAGSDEKCAACVKLGAAEAFNYKSQDFVAGVKAATGGKGVDVILDMAAG
                     DFVPRELKALADDGRLVFIAFLRGAKTELDVNELMRRRLTMTGSTLRPRPVAFKGAIA
                     RSLRERIWPLIEAGKIKPVIFRTFPLAQAGEAHKLMESSQHIGKLVLTV"
     gene            14803..16707
                     /locus_tag="VFB53_02525"
     CDS             14803..16707
                     /locus_tag="VFB53_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HZN85637.1"
                     /translation="MARALITACLFILGSAAAAHEAAPLPGADGRVALGAHVEALEDP
                     GGALRLPDVRGAQHAARFARLDDGGVNFGYTRSAWWLRFALPGGAPAGEELLLEVAYP
                     SLDRVEFHLPEALPGGEARYWARVAGDQFPWAAREIPHRNYLFRFAAPAAAGAHYYYL
                     RVESRSVLTVPLTLWRPEAFARHDRDTQLVLGLFYGLGAAMLLYNLMLFFALRDRMYL
                     YYVLYVAAFGLFLFGFNGLAYQYLWPQSPWGANNLVAATLALALALGAQFARAFLDSR
                     RIAPLADRAMLAIVVAGAVLAGCAATGWGLDYGGVLRVVTVLGLATAAAATGIALAAL
                     REGYRPARYFLLAWAALLAFIALGALRNFALVPTNFLTVYGMHIGFALDVLLLSFALG
                     DRIRMLRRENVAAHAEALESQQLLLEATRESERALETRIAVRTAELNRANERLRAEAA
                     DREQLMEQLQKQEEHLRFMAQHDPLTGLPNRTSMQQRLALAMELAKRNRKKVAVMMVD
                     LDDFKRINDTRGHPAGDQALVQLAARLRTSVRGSDTVARYGGDEFVILAGELDRAADA
                     AMVAEKVADMVSVPLPVQGGLEKVSCSIGISVFPDDAEEGEALIALADKAMYGSKAAQ
                     GRRYAFFGAA"
     gene            complement(16704..17660)
                     /locus_tag="VFB53_02530"
     CDS             complement(16704..17660)
                     /locus_tag="VFB53_02530"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HZN85638.1"
                     /translation="MEVTFDDIAAAHERIRPYARRTPVLSSATIDALTGAEVHFKCEN
                     FQRMGAFKFRGGYNALAQLSPEQKRKGVVAFSSGNHAQAVALSGKLLGVPAVIVMPTD
                     APPVKLAATRGYGAEVVLYDAGNEDRGKIAEQLAAERGLTLIPPFDHPHILAGQGTAA
                     KELIEDTGPLDYLLVPCGGGGLLSGCAIAAQHLSPRCRVIGVEPAAGDDGLRSFRAGR
                     LESVPLPMTIADGARTMSLGKLTFSIIRANVSDFLTVTDEELLRWMFYLWERMKIVVE
                     PTGALAAAALLQGKIDAKGKKVGVVISGGNVDLAWAARQLAR"
     gene            complement(17651..18517)
                     /locus_tag="VFB53_02535"
     CDS             complement(17651..18517)
                     /locus_tag="VFB53_02535"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HZN85639.1"
                     /translation="MRTPDARFADLPGFAHAPRYWEWRGLRAHYVEAGRGERTWLCLH
                     GEPTWSYLYRRMIPPFAAGGARVVAPDFLGFGRSDKPAEESFYTFDMHRTFLLDFIER
                     LDLTRIALVVQDWGGLLGLTLPMEMPERFERVLLMNTTLGTGDAPLSEGFLAWRAYVS
                     RTPDLPCDKLMQRSCRHLSEAEAAAYEAPYPDSASKAGARAFPNLVPDRPDAPGAALS
                     RQARDWWMHAWKGESFMAIGARDPVLGPPVMHALRRVIRDCPEPYVHAEGGHFLQEWG
                     DDVAREVLQRWK"
     gene            18541..19350
                     /locus_tag="VFB53_02540"
     CDS             18541..19350
                     /locus_tag="VFB53_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85640.1"
                     /translation="MDYSKYQDLNCRLLEPGILEIVMGRDDGRLSTAGHRMHAELAEI
                     WRDVDRDPAVRVAILRGAGKGFSAGGDFSLIDDIIRDFDTRARVWREARDLVYNLINC
                     SKIVVSAMHGPAVGAGLVAGLLADISIAAKNARIIDGHTRLGVAAGDHAAIVWPLLCG
                     MARAKYYLLTCEQLSGEEAERIGLVSLCCEEAQLQAKALEVARKLAAGAQTALRWTKA
                     SLNNWLRMAGPAFDNSLALEMLGFSGPEAKEGAASHREKRKPEFPPAPKGF"
     gene            complement(19347..19619)
                     /locus_tag="VFB53_02545"
     CDS             complement(19347..19619)
                     /locus_tag="VFB53_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85641.1"
                     /translation="MEISAQQLAELLIGIARAQTAVIQGIDSAMAGIKTQSIVPMLQN
                     AAHLRDHPEPSLVDLPVRVLLTTQGRVPPDPAAVAKDLERLLAPKA"
     gene            19670..20170
                     /locus_tag="VFB53_02550"
     CDS             19670..20170
                     /locus_tag="VFB53_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZN85642.1"
                     /translation="MLRRSLAAVLLLVLGAAAGLALHLATARGDTMFAGKRPGYLGVK
                     DGRLARPKSTPNCVASQADAADGEHYVAPIRFKGSALEAIAAARKAVESMARATVVRH
                     EGNYLYAEFKSRLMGFVDDVEFTYDEKAGVLHLRSASRLGRRDFGVNRARAEALRARI
                     EGRINT"
     gene            20182..21171
                     /locus_tag="VFB53_02555"
     CDS             20182..21171
                     /locus_tag="VFB53_02555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZN85643.1"
                     /translation="MAAFPRMLLLVLALGAAPAHAQDKPKDDADELLAAVVQVKAKIL
                     PDARSLATLGPQRQGSGVLVREGYVLTIGYLVIEAEAIEVSGAGGKTVPATLAAYDHA
                     SGFGLLRLAAPLAARPLPLGDASALAEREPAMIAAHGGAEGLNLVYVVSRRPFSGSWE
                     YQLDAAIYTYPAVQNWSGAALIGPKGDLLGIGSLIVPDAGGPGTQSPGNLFVPIDLLK
                     PILEDLIAKGRSAGPQRPWLGIYAEELRGRLFVQRVAPEGPAERAGLKAGDLVLGVGG
                     EAVGSLAEFYRKLWARGAAGVEVPLRVLQGLQLKELSVRSIDRLEYYRRKPSY"
     gene            complement(21165..22070)
                     /locus_tag="VFB53_02560"
     CDS             complement(21165..22070)
                     /locus_tag="VFB53_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85644.1"
                     /translation="MRRNDHDVSGRIRTTEPAAVGAEVARLFQDLYPQASPRPIERAF
                     ADTVRLYRGAHPRFHACDTPYHDLQHVLDVTLAQARLMDGYERSRSGPPPLPAPLFTV
                     GVICALLHDVGYLRRRADRRHGNGAEYTLRRASRGAGFVAGYLRGAGLARYAASAAPL
                     LHYTGYERSAESIRVDHPVLRRIGQMLGSADILAQMSDRCYLEKCRDRLYPEFVAGGV
                     ARALYRSALELVRRTPAFFHNAAQRLDLQLARAHEYAARHFGGQNLYLEEIHKNVRHA
                     RLVADSGRDELLRRAPPAAPALAAQ"
     gene            22203..22424
                     /locus_tag="VFB53_02565"
     CDS             22203..22424
                     /locus_tag="VFB53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZN85645.1"
                     /translation="MSNLPLTEEERAQIEARIHALEVEHSDLDDVIAHLVEVPVQDQL
                     QLRRLKKRKLLLKDLIARLRDRLIPDIIA"
     gene            complement(22414..22818)
                     /locus_tag="VFB53_02570"
     CDS             complement(22414..22818)
                     /locus_tag="VFB53_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN85646.1"
                     /translation="MASRFAVSHRARSRFVRRGLRNYFEYRDLGIKRATRGKVVAHVI
                     RARPGKAPHGQWHRHLCEVQFVYVLKGWAVFEYEGVGKVRMKAGSCFYQPPGIRHREI
                     SHSRDLELIEIVAPADFRTLDAPPPRRARVKR"
     gene            22864..23934
                     /locus_tag="VFB53_02575"
     CDS             22864..23934
                     /locus_tag="VFB53_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN85647.1"
                     /translation="MFDQFIGVKPVEERHRFDVGRLARYLRGKIDDWQGELKVEQFRG
                     GQSNPTYRVTAGAKRYALRRKPPGKLLPSAHAVDREYKVIKALHGTGFPVARPYLLCE
                     DEAVVGTAFYVMDCVEGRVLWDQALPGMTPAARGAIWDELNRVIALLHGIDWKAVGLE
                     DFGKPGDYIARQVSRWTKQYQASELEKIEEMDRLIAWLPQHIPAGDETAVVHGDFRLD
                     NTVFHPSEPRILAVLDWELSTLGHPLADFAYHCMSWHIAPGKFRGIAGLDLAALGIPG
                     EREYVERYCRRTGRAGIDPSTWDYYMAYNLFRIAGITQGIAKRVVDGTAASEYAREAG
                     SRTRPLAELGWAQVEKILQRAA"
     gene            23945..>24933
                     /locus_tag="VFB53_02580"
     CDS             23945..>24933
                     /locus_tag="VFB53_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN85648.1"
                     /translation="MDFEYSPRVKEMQQRLKAFMAEHIYPSEPAYYAHTRSDRRWEPV
                     PVIEKLKPLARKAGLWNLFLPHSKRAPEGLSNLEYAPLCEIMGRTSWSPEVFNCSAPD
                     TGNMETIERYGTEEQKKQWLEPLLRGEIRSAFLMTEPAVASSDATNIQMQIRRDGNDY
                     VINGRKWWSSGAGDSRCKVYIVMGKTNPDAASRHSQQSMILVPAGAKGITILHHLNVF
                     GYDDAPHGHMEIEFKDVRVPASNILLGEGRGFEIAQGRLGPGRIHHCMRLIGQAERAL
                     EKMCKRATERVAFGRTVAEQGVTRERIAEARILIDSTRLMVLHAAWRMDTVGN"
BASE COUNT         3377 a         8739 c         9010 g         3807 t
ORIGIN      
        1 cgcatgtcgc gtatcacgct catgacgtgc tccgcgcgtt gcgggtagtg ctcggcgagc
       61 cattccctga agaggtcctt cagctcgttg ggcaggcgca tgatgacata ggccgcttcg
      121 gccgccccgg cctgcgccgc cgcctcgagc acgtgctcca tggtcttgtc gttgagcgcg
      181 ggaacgagcg ccgccaccat cacgccgcag ggcaccccgc tgccgttcag gttgcgcacg
      241 acgtccagcc tgcgctgcgg gctggccgcc cgcggctcga gctttcgcgc gagctcgcgg
      301 tcgcgggtgc cgatggagac gtacacccgc acgaggtttt tccgcgccat cggcgccagg
      361 atgtccaggt cgcgctccac cagcgccgac ttggtgacga tggtgagcgg gtgctcgcag
      421 gccgagagca cttccacgat ctggcgggtg atgcggtgct tgcgctcgac gggctggtag
      481 cagtcggtat tggcgccgag cgagatgggc gataccacgt agccgggctt cgccaattcg
      541 tccttcagga gctccgccgc attcgtcttg gcgaacagcc tggtctcgaa atccagcccg
      601 ggggagagtt ccaggtaggc gtggctcggg cgcgcgtagc agtagatgca gccgtgctcg
      661 cagccgcgat aggggttgat cgactggtcg aaggggacgt ccggggaatc gtttttcgag
      721 atgatcgtgc gcgcccgctc gatggtgacg ctggtctcga gcggctgcag cgcttcgtct
      781 tcccgcgccc agccgtcgtc gaacgggtcg cgcgcctcgg tctcgaagcg tccctggatc
      841 tgcgaaagcg cgccgcgctg gcgttgcgtt tccatctaga atggcaatcc ctcccgatac
      901 tgtatacatg aacagtaccc gccgcaatgt cggactgctg gccgcctgcc aggcgctgct
      961 gctgagcaac aacgcgaccc tgatcgccat caacggcctc gccggattcg cgctcgcgcc
     1021 gaacgccgcg ctcgccacgc tgccggtgac ctgctgggtg gtgggcgggg cgatcgccac
     1081 catgcctgcg tcgctgcaca tgaagcgcgt cggccgccag cgcggcctga cgctcggcat
     1141 ggcctggggc atcgccggcg cgctgttgtg cgcggcggcg gtgtggcagc agggcttctg
     1201 gctgctgtgc ttcggcacgc tcgtgttcgg cgtgttcaac gcctacggcc agtactaccg
     1261 cttcgtggcg gccgacgtcg cggggcccgg cttcaagagc acggcgattt cactggtgct
     1321 cgccggcggg ctggtgggcg gcatcctcgg accgggcctg agccgcgcca ccgtggacct
     1381 gctcgagccg cgctacagcg gggcctacct cggcctcatc gcgttctcgg cgctcagcat
     1441 gctgctcctg cgcttcatcc gcgtgccggc gccggatgcc agggtgcagg ccgcggcggg
     1501 ccggccgctg gccgagatcg cggcgcagcc caagttcatc gtggccgtgc tctcgggcgc
     1561 catcagctac ggcgtgatga atttcctcat gacctcgacg cccatcgcca tgggcatctg
     1621 cggccacccc tatggcgacg cggccttcgt catttcctcg cacgtgatcg ccatgttcgc
     1681 gccttcgttc gtgactggca gcctgatcaa gcgcttcggc gtgctgcagg tgatgttcac
     1741 gggggcgctg ctcaacttcg ccgccatcgg cgtggcgctc tcgggcatcg cggtggcgca
     1801 gttctggtgg tcgctggtgc tgctcggcgt gggctggaac ttcctctatg tcggcggcac
     1861 cgcgctgctc accgagacct accggccgga ggagatggcg aaggcgcagg gcgcgaacga
     1921 acaggcgatc ttcgtcatga tggcgatctc ctcgttcacc tcggggctga ccgtcaccgc
     1981 ggcgggctgg gagcgcgtga atctctatgc gctgccgatg gtggcagcgg tggcgctcgc
     2041 catcctctgg tacggcatca cgcagcgttc ccgcgccgca tgaacgcccc cgcgcaggtc
     2101 ggccagcgcc tggaggaggt cgacaccccg gcgctgatcc tcgagctgga cgccttcgag
     2161 gccaacctgc ggacggtggc ggacctcgcg cgcggccgcg tgcgggtgcg cgcgcacgcc
     2221 aagaccagca aatgtcccga ggtcggcagg cgccagatgg cgctcggcgc ggtgggcgtg
     2281 tgctgccaga aggtgtcgga ggccgaggcg atggtcgccg gcggcatccc ggacgtcctg
     2341 gtgtcgaacg aggtggtggg cgcggccaag ctcgcgcgcc tggcgcgcct cggcgcgcgc
     2401 gcgaagatcg gcgtgtgcgt ggacgacgcg ggcaacgtgc gggacctgga cgccgcggcg
     2461 cgcgccgccg gcgcgacgct ggacgtctac gtggagctgg aagtcggcat ggggcgctgc
     2521 ggcgtcgagc cgggcgcgcc ggcgctcgcg ctggcgcgcg cggtggcggc cgcgcccaac
     2581 ctgcgcttcg ccggcctgca ggcctacaac ggccgagcgc agcacgtgcg ctcgaacgcc
     2641 gagcggcgcg cgctgatcga gaaggccgcc gcggcggtgc gcgccacgcg cgcgctgctg
     2701 gaggcgaacg gcatcgcctg tcccctcgtc accggcgccg ggagcggcac cttcatgttc
     2761 gagatcgagg cgggcgcctg ggacgagatc cagccggggt cgtatgcctt catggacgcg
     2821 gactacgcga agaacgaatg ggcggcgccg ctgccgcgct tcgcgcacgc gctgttcgtg
     2881 ctcaccacgg tgatgagccg cccgcggccc gaccgcgcca tcgtcgatgc gggcctcaag
     2941 gcctcgagcg tcgattcggg catgcccgcg gtgtggcagc gcccggggct cgcctacgcg
     3001 ctcgcctcgg acgagcacgg cttcgtcgag gtggcgccgg gcgcgccggc gccggccctc
     3061 ggcgagaagc tgctgctggt gccggggcac tgcgatccga cgatcaacct gcatgactgg
     3121 tatgtttgcg tcaggggcgg caaggtcgag gccctgtggc ccatcaccgc gaggggggcg
     3181 ctggcatgaa aaagcgatat ctcaacccga aggaactttt cgatccgcga tttttcaccc
     3241 acacggtggc ggtggagggc gaattcaccc tcgtgtacgt cgccggccag gtgagctacg
     3301 accgcgacgg cgtggtggtc ggcaagggcg acatgcgcgc gcaggccgag caggtgttca
     3361 agagcctgac gcacaacctg aaggccgccg gcgccgcctg gagcgacgtg gtcaagctga
     3421 acggctacat ggtgcgcatg agcccggagg cggtgaacca gtaccgcgag gtgcgctcgc
     3481 gcttcctcga ccccaaggcg ctgccggcga gcaccctgat cggcgtcgag cgcctggtgc
     3541 acgacgacct gctgctcgag gtcgaggtgg tcgccgcggt cgccgcgcgg cccgcagcga
     3601 aatccgccgc caagcgctga cttgagccgc gaccgttccg gctgggtggt ggtggcctgc
     3661 gccttcaccc tgatgttcgt cggcttcggc gtcgcgtatt cctttgccgc cttcttcaag
     3721 gccttccagg ccgagttcgc cgcctcgcgc gcccacgtgt cgctggtgtt ctcgctgtgc
     3781 gcgctgctgt ggttctccat cggcgtgccc ggcgggctgc tcgccgaccg cttcgggccg
     3841 cgcggcgtgt gcctggcggg cgcgctgtcg atcgccgcgg gactggccgc ggccgcgcac
     3901 gcgcactcgc tcaccgtcct gtacgccacg tacagcatcg gcatcggcgt gggcatcggc
     3961 ctggcttacg tgccctcggt cgccgcggtc cagcactggt tcacgcagca tcgcgtgctc
     4021 gccggcggca ttgcggtctc gggcatcggc gcgggaaacc tgctggtgcc gccactcgcc
     4081 gcctggtgga tcgagctgtg gggctggcgc ggcgcctacc tcgggctgat gtggctcacg
     4141 ctgctcgcgg cggtaccggc ggccctgctg ctgggcgggc gcgccgcgac gcgcgccgcc
     4201 gcggccgggg ccacgttgcc cggcatgtcg ctggccgagg cgatgcgcag ccgcctgttc
     4261 tggctgatgt acgcggtggc gttcctcgtc tgcgtgggat tcttcgtgcc gatggtgcac
     4321 ctcgcgccct acgcgcagga cctcggcctc agcgaggcgc agggcgtgag cctcgtcagc
     4381 ctgctcggcc tggggagcct cctcgggcgc ttcctgatgg gcggcatcgc cgagcggctg
     4441 gggcacgcgc gctcgctggt ggcgatcggc gcgggactcg gcctgctgtt cctcgtctgg
     4501 tgggtggcgc ggagctactg gctgctcgcc gcgttcgcgg tgtccttcgg cacgctgtac
     4561 ggcggctacg tggcgctcgc gccgacgctc agcatggagc tcttcgggcc gcgcagcctc
     4621 tcggcgatcc tcggcgcgct gtacaccgcc gcgggactcg ccaccttgat cgggccgact
     4681 ttcgccggcg cgagcttcga cgccaccggc agctacgacg cctcgatcct cggctgcgcg
     4741 gcgctgtcct tcgccggcgc ggcgatcgcc ttcggcctgc gcgcgcattt ccgccccgcg
     4801 gcatgagccg ccggccggcg ctcgcggtga gcgaggagcg cggcgtgcgc acgctgcaca
     4861 tcggcggcga ggcgatccag agctcgatgc ggctgtcgga cccctatgcg ctggagctcg
     4921 actacacgcg ctgcatgatg gccttcctgc tgttccaccc gcgccccgcg cgcgcgctga
     4981 agatcggcct gggcggcggt tcgctgccga agtacttctg gcggcgcttg ccctcgctgc
     5041 gcacgcgggt cgtggagctg gacgagcgcg tggtggcgat ggcgcgcgag cgcttctgcc
     5101 tgccgcccga cgacgcgcgc ctgcgggtgg acgtcggcga cggcgccgag gcgctcgcgc
     5161 cggagtgctg cgacctgctg atcgtggatg ccttcgccga cgagcacccg ccggcggcgc
     5221 tggtgagcca ggcgttcttc gacgcggcct ggctcgcgct cgagtcgccc ggcgcgctgg
     5281 tgctgaacct gatggacgac gacccgaagc tcgacgagcg cctgcagcgc gtggagcgcg
     5341 ccttcggcgg cgcggtgctc gcgttccacg cgctcgtcga cccgaacgtg atcgtcatcg
     5401 cgctcaaggg cgcgccgccg cgcctcgcct gggacctgct caggcgtcgc gcgcgggccc
     5461 tcgagcagcg ctacgggctg ccgttcacgc gcatgctggc gcgcttccgg cgcatgaacc
     5521 gctgcgacgc cgaggcactt ataatcgccg tgcaggggga cgcggcatga aactcgagag
     5581 cgacagcttc atcgaccagg gcgcgattcc ggggcgctgc gccttcgcgg tgcccgacgc
     5641 ggcggcgcac gtcaggctct ccgacaaccg caacccgcac ttcgcctgga agaacctgcc
     5701 cgcgggcacg ctgtcgctgg cgctggtgtg ccacgaccgc gacgtgccct ccaggggcga
     5761 cgacgtcaac caggagggcc gccaggtgcc ggcggacctg ccgcgcgtgg acttcttcca
     5821 ctggctgctg gtggacctca agcccgaggc cgggccggtg cgcgagggcg agttctccaa
     5881 cggcgtcacc gcgggcggca agtccggccc ggcgggcgcg cgcggcacgc gccatggcgt
     5941 caacgactac acggcctggt tcgccggcga caaggacatg gcgggcgact actacggcta
     6001 cgacgggccc tgcccgccgt ggaacgacac catcgtgcac cactacgtgt tcacgctgta
     6061 cgcgctggcg gtcgagtcct gcccggtggc aggcgcgttc cgcggccccg agctgctcaa
     6121 ggcgatcgag gctcacgtgc tgggcagcgc ggcgctcacc ggcctgtatt cgctcaaccc
     6181 ggcggtgaag acctgagcga gctgctcggc gggcagtggg agctgctcgc cctcatcccg
     6241 atcgcgttcg ccggcgccct ggtgttcggg ctgaccggct tcggctcggc gctggtcacc
     6301 atcccgctcg ccacccactt cgtgccgctg cagttcgcgc tcgccctgtt cgcgctcgcc
     6361 gacctggcga gcgcgctgcg cgtggggctg gagaacccga ggagcgcggt gcgcgcggag
     6421 tggacgcggc tggtgccgat gatcctggtg ggcaccgcgc tcggcgtgac gctgctggtc
     6481 aacatgccgc gcgcggccgg gatgctcgcc ctgggcgcgt tcgtgatcgg ctacgcgctc
     6541 tatagcctgc tgcggcgtga gtcgccgcgg atcgtggcgc cgggctgggc ctgggtggcc
     6601 ggccttgcag gcggtgtcac cagcacgctg ttcggcgccg gcggcccgcc ttacgcgatc
     6661 tacctgtcgc agcgcggcct cagcaaggag cagttccgcg ccaccatggg attcgccacc
     6721 atgaccagca tctcgctgcg cgtggccgcg ttcctcctca ccggcctgtt gctcgacccc
     6781 agggtctggc tggtggcgat cgcggtggtg ccggcggcgc agctgggcat cgtcgccgcg
     6841 cagcgcatgt tcctcaggat ctcgcgcgag cagctgctgc gggcggtggc gctggtgctg
     6901 ctggcgacgg gggggacgct ggtcgcgcgc gcgatcggct gattcagccg ccgtacatgt
     6961 agtcgcgcgt caagggcagg gggttgcgcc tggcgtcgct gctgcgcacc gccagcacct
     7021 gctgcaccga cacccagccg cgctcgaagg cgtgcgcgca cccggcgagg taggtgcgcc
     7081 agatgcgcag gcgcttctcg cccgcgtagg ccttcgcctc ttccagcctg gcctccagcc
     7141 ggtcgctcca ccggtgcagc gtcttggcgt agtgcaggcg cagcgtctcc gcgtccatca
     7201 cttccagccc ggccgcgccc atctcgcgca gcaccagcga caggtgcggc agctcgccgt
     7261 gcggaaacac gtacttctgg atgaattcgc ccccgcccat gcccaccgcg cggctgtccg
     7321 ggtccaccga ggtgatgccg tggttgagca cgatgccgcc gtcggcgagc aggcgccgga
     7381 tggcgccgaa gtacaccggc aggttcttca gccccacgtg ctcgaacatg ccgatggagg
     7441 cgatcttgtc gaacacgccc tcgccgggca cgtcgcggta gtcctgcagc aggacccggc
     7501 agcgctcctg cagcccggcg gcgcggatgc gctcgttggc gaggtcgtgc tggttctgcg
     7561 acagggtgat gccggtcgcc gaggcgccgt agcgctcggc ggcgcgcatc accagcgccc
     7621 cccagccgca gccgatgtcg aggaagcggt cgccgggctg caggcgcaat ttgcggcaga
     7681 tgtggtcgag cttgcgctcc tgcgcctcgt cgatggtctc ggcgccgctc ctgaaatagg
     7741 cgcaggaata gaccatgcgc cggtcgagcc acagcgcgta gaaatcgttg gacacgtcgt
     7801 agtggtagtg gatcgcctcg gcgtcgcgct ccttggagtg cgcgctgcgc agccagggca
     7861 ggcgcgcctt gcgcgccggg ccccatttca gcgccaggcc ctcggccacg cgcatggcgt
     7921 cggcgatcgg cccgtcgact tcgaggtggc cttcgacgta ggcctcggcg agcttggcga
     7981 ggtcggcgtt gacgaagtag cgcagggagg cggcgtccgg gacgcgcagc gtgacggtcg
     8041 gccgctcgcc gaggtcgtag cgcttgccgt tccacaactg gatggcggcg ggcaggctcg
     8101 ccccggcgcg gaactcgccg accatcttct cgatgcgcgc ttcgagcatc agaccacccg
     8161 ctaggtcgtc ttgtccctca tggtaacgaa ttcctccgcc gcggtgggat ggatgccgat
     8221 ggtgtcgtca aactgcgact tggtggcgcc gagcttcacc gcgatggcga tgccctggat
     8281 gatttccccg gcgtccggcc cgatcatgtg cgcgccgagc acgcgctgcg tcgcagcatc
     8341 caccaccagc ttcatgaacg tgcgctcctc gtgcgcgccc atcacgtgct tcaaggcgcg
     8401 gaacgaggtc ttgtagatgt ccaccttgcc gtgccgcgcg cgcgcctgct cctccgacag
     8461 gcccacggtc gcgaggttgg gattggagaa caccgccgtg ggcacgttcc ggtgatccgt
     8521 cgccgtgggc tcgccgcgga acagcgtctt cgccagcgcc atgccctcgg cggtggccac
     8581 cggcgtgagg ttgagccggt tggtcacgtc gccgatggcg tggatcgact ccaccgagct
     8641 cctcgagtac gcatccacca ccaccgcgcc gtcgccgccg agccgcacgc ccgcgccttc
     8701 caggccgagg cccgcggtgt tgggcttcct gccggtggcg aacatgacga gatcggtttc
     8761 ctcggccgcg ccgtcctcgt aggcgacccg gatgcccggc tcgagcttcg cgggattggc
     8821 ctgcaggcgg atggcgacgc ccttcttgcg catttcctcc gcgaggcgct cgcccagctc
     8881 ggcgtcgaag ccgcgcagca ggcgcgcgcc gcggtaggcg agcgtggtct ccacgcccag
     8941 tccctggaag atcgaggcga actccaccgc gatgtagccg ccgcccacca ccagcgcgcg
     9001 ccgcggcagg cgcgcgaggc cgaaggcctc gttggaggtg atcgccagct cgcgtcccgg
     9061 gatctgcggc acctgcggcc aggcgccggt ggcgacgagg atgtgcctcg ccgtatgccg
     9121 cgcgccgccg gcctcgaccg tgtgcgcatc gatgaccctg gcgcgcgcgc gcaggatctg
     9181 cgctcccgcc ctggcgagcg cgccctcgta cacgccgttc aggcgcgcga tctcgcgatc
     9241 cttgttggcg agcagcgtcg cccagtcgaa gcgcggctcg ccgacggccc agccgaagcc
     9301 cctcgccacc tcgaaatcct cgcggaagtg cgcggcgtag gcgaagagct ttttcgggat
     9361 gcagccgacg ttgacgcagg tgccgccgaa gcggccgtcc tcggcgacgg cgacgcgcgc
     9421 gccgtaggac gcggcgaagc ggctggcgcg cacgccgccc gagccgccgc cgatggtgaa
     9481 gaggtcgaag tcgaacttca gctggtgagc gcgcgccgct tatttcaggt tggggaggtt
     9541 gatctccacc agctcgagct gggcgacgag gttcttgtgc tcgggcttgt cggcgagcca
     9601 cttgaccgcg ttgcgcacct tctgctcgag ctgcgcgcgc ggcgcgccct cgatgccggc
     9661 gaacatctgc gccaggtcgg cgaggctggc ggcctgcgcg gcggcgcgca cctggccgag
     9721 ccgctcctcg atgccggcgc gatcgaggtt ggtcaccagc ttgagggtct tgtcgtgcgt
     9781 gctcatcgcg cgctccttcc gatgtcaggc gattgtacca gcgtgtagcg gcccgccgca
     9841 gcgtccacgc gcccctcggc ggcgagcttg ccgaggtgcg cgctgagcga gcgcgccgcc
     9901 accgggtgca ggcgcggctg cacgtcgtcg tacacggtgg ccaccagctc ctccagcgtc
     9961 ggcgccgcca gccgcgacag cgcgttcagc accttggtct cgcgcgccag gcggtggcgg
    10021 atcagccggc gcgcctcgcg gtgcggctcg ccgatgaggt agccgtggcc cggcgcgagg
    10081 atcaggatgt cctcggcgag cagcatctcg agcgaggcga ggtaggcgcg catgtcgccg
    10141 tcgggcgggt tgatcaccac cgtggagccc tgcatcacgt ggtcgccggt gaacagcatg
    10201 cgcgtgttct ccagcaggta gcacaggtgg ttggaggcgt gtcccggggt gtgcagcgcg
    10261 cgcagcgtga ggccgcccat catcaggcgg tcgccgtgcg ccagcacgcg ctcggggacg
    10321 aagctgcggt cctggttcgc ctgctccggc gccggccggc cgatcagcac cgcgccggtg
    10381 gcggccttga tcgcgcgcgc cgcgggcgaa tggtccatgt gcgtatgcgt gcacagcacc
    10441 cagcggatgc ggcccgcgcc ggcggcgagc accgcggcga tgtgcgcgtc gatggcgggg
    10501 cccgggtcga tgaccgcgag ctcctgctcg ccgaccaggt aggtgttggt gccgggcccg
    10561 gtcatcatgc cggggttggg cgcgatgagg cgcgtgacgt actggtcgag gcgcttcggt
    10621 ctgcccggct cgagcgcgac gctggtctgc ccggtctcgt ccggatcgct ccagtggatc
    10681 tcgaagtact gcgggtcgcg gcgccggtag agcgccgccg cgccgtccgc gccgagcgcc
    10741 cagcaggcgc ggttggtctc gacctccgcc tcgcgcgcgt gggcgagcgc gtccttggcg
    10801 cgcgcgaaac gcgtgaggtc cgccagcgtg ttgcgcgtgg cgaacaccag ttccatctcg
    10861 ccgcgcgcct cgcgctcgat cgcctcctgc ggccgcagcc actggctggc gacgagctcg
    10921 gcgccgtcgt gcgagcccgc ctggccggcc ggcgccagcg ccaggaaaaa gcgcgtgtcg
    10981 aagcgccgcg cccggcccgg cgcggtgatc cagtggccga agtaggcgag gtcgcccgcg
    11041 cgcagcagca ggcgctcctt ttccaggaac tcggcgaagc cgagcttgcc gccgtgcagc
    11101 agcggccggt agggcgccag ttgcgcggcg cgcgccgcgt ccaccgggcc gttctccccc
    11161 tcggcgagca ggatgccggc ctcctcgaag cattcgcgca ccgccgccac ccagtaggcg
    11221 aggccgccgc cggccatgcc gaggcgcgcg ctcgcctcgg cgtccgacag gcccgccacg
    11281 cggcgcagca cgcgcgggtc ctcgtcggcc ttgtcgagcg cgccgcccgg gaacacgtgc
    11341 gccccggcga ggaatgccgc gcccttcgtg cgctgcagga ggaaggcttc gggccccagg
    11401 ctgccgtcgc gcagcaggag cacggtggcc gcgggacgcg cgacgggttt ctcggccatc
    11461 ctcaggcggc gcggccggcg cggtccttgt cgttctgcgc cttgatcagc tcgcgcgcct
    11521 tcgcccagtc ggcgtccgac atcttgtcga gctcggtgaa ataggcgccg gtggcgaggt
    11581 agccgccgcc gtcgatggcg atggtctcgc cggtgagcca ctcgcagccc tcggccatca
    11641 ggaaagtggc gagattgcgc agctccgaca tctctcccac gcggcgcatc gggttcacct
    11701 tggtcgactc gccgaagctt gcgccgggac gcaagcgcct ggtggcgccc tcggtgggaa
    11761 aggggcccgg cgcgatggcg ttgagccgga tgccgtgctt gccccactcg acggcgagcg
    11821 acttggtcat cacgtgcagc ccggccttgg acatcgccga gggcacgaca tagggcgagc
    11881 cggtccacac ccaggtgacg aggatcgaga tcaccgagcc cttgtgcccg ccctcgatcc
    11941 agcgcttgcc caccgcgtgc gtgacgtaga agctgccgtg gaacacggtg ttggcaatgg
    12001 cgttgaagcc gttcggcgtg aggtccttgg tcgggctgat gaagttgccc gcggcgttgt
    12061 tcaccaggcc gctgagcggg ccggattcgt cccagatgcg ctgcgccatg gcgtccacgg
    12121 cggcatggtc gcggatgtcg acggcgtgcg cgcgcacctt gccgccgtgc tgcgccatca
    12181 gctcccgcgc cgtggcctgc agcacgccgt cgcgccggcc ggcgatccac agatcggcgc
    12241 cgagcgcgag gtactgggcg gcgatctccc ggccgaggcc ggtgccgccg cccgtgacga
    12301 ggatgttctt gcccttgagc aagccgtcct tgaacatgcg cttctccttg cgggaagcgc
    12361 gtattctagg tcagccgcag gcgtcggtgc gaagcgccgg ggtgcgctgc aggttcaggg
    12421 cccgtgtcga ctgctcgaaa aagcggtgca tggcttcggc gcgctcgcag tgcgcgcgcc
    12481 gctcgagctc ctcgcgcagc ccttcgtcct gcaggtagcg ttccagggtc agttccatgt
    12541 gcgttctcct cggggtcagg cgagcgccac gctggcgtcg ccgaaggcat gcaccagggc
    12601 gaggcccggg cggcgcagct cgtagcagcc gcccgcttcg agcaccacgt ccttgggctg
    12661 gttctcttcg gtgatccaca cgctgccctc gctgacgcag atggtgctgc cctgggcgcc
    12721 ggtcacccgc agggtctgcc cgcgggcgag gcgcagggcg ccgggttgca gttcgatcct
    12781 catggtcggt ctccgttcgg ttgcgtttga catgcgcatg gtgcattctt gtcggcggcg
    12841 cctcaaacgg taaaacctca ggacatgcat gcgtaacgag aatgtgagac tgccctccct
    12901 ggacctgctg cgcggcttcg aggcggcggc gcgcctgctg tcgttcacgc gcgccgggga
    12961 ggagctgcac ctcacccagt cggcggtcag ccggcagatg caggagctcg aaggccagct
    13021 gggcgtgaag ctcttcgagc gccgccaccg cgccctgtca ttgaccgacg cgggccagca
    13081 gttctacccg gcggcggcgc aggtgctcgc caccatgcgt gccgccaccg accggctgcg
    13141 ggcgcagtcc gggcgcggca ccctgtcggt caccaccacg cattcgctcg cggcgctctg
    13201 gctcattccg cgcctggcgg gcttcaccaa ggcgcatccc ggcgtggacg tgcgcatctc
    13261 ggccgacacg cgcgtgcagg acctggagcg cgacggcctc gacgtggcga tccgccatgg
    13321 cccgccacag ctcgcgggcg agggcgcgac gcgcctgttc ggcgagcgcg tgttcccggt
    13381 gtgcagcccg aagttgctga aggacgcgaa gaagccgctc aaggtgcccg cggacctgcg
    13441 ccaccacacc ctgctgcagt acgacgaccc cgatgcgcgc catccctggc tgcactggcg
    13501 cacctggctg gaagtggagc gcctggccga cctgaagccg gcgggacggc tgagcttctc
    13561 gggctacgag cagatcaacg cggcggcgat cgcggggcac ggcgtggcgc tcggcaggag
    13621 ccccctggtg aagggctcgc tcgcctccgg ggagttggtg gcgccgttca agcgcactgc
    13681 cgacccggca cgcggctact tcgccgtcgt gtccaggaat tcggcggagc ggccggaagt
    13741 gcgggacttc gtcgcctggc tgctggaaga ggctaaacgg taagcaccag cttgccgatg
    13801 tgttgcgagg actccatcag cttgtgggct tcgccggcct gcgcaagcgg gaaggtcctg
    13861 aagatcaccg gcttgatctt ccccgcctcg atcagcggcc agatcctttc ccgcaggctg
    13921 cgcgcgatcg cgcccttgaa cgccaccggg cgcgggcgca gcgtcgagcc ggtcatggtg
    13981 agccgccggc gcatcagctc gttcacgtcg agctcggtct tcgcgccgcg caggaaggcg
    14041 atgaacacca ggcgcccgtc gtcggccagg gccttgagct cccgcggcac gaagtcgccc
    14101 gccgccatgt cgaggatcac gtcgacgccc ttgccgccgg tggcggcctt caccccggcg
    14161 acgaagtcct gggatttata gttaaaggct tcagcggcgc cgagcttcac gcaggcggcg
    14221 cacttctcgt cggaacccgc ggtggcgaag acgcgattgc ccagggcgct cgccatctgg
    14281 attgcggcga cgccgatgcc ggaggtgccg ccctgcacga ggagggtttc tcccggcgcc
    14341 agccgccccc ggtcgaagac gttcgaccag acggtgaaga aggtctccgg caacgaggcc
    14401 gcctcgacca tcgacaggcc cctgggcacg ggcaacgcct gcacttccgg ggccacgcag
    14461 tattcggcat agccgccgcc gtgggtgagc gcgcacaccg catctcccgt cttccacatc
    14521 gtcaccgcgc tgccgcaggc gacgacttcg ccggcgatct ccagccccgg caggtcggag
    14581 gcgtccggcg gcaccgcgta caggcccatg cgctgcagca cgtcggggcg gttcacgccc
    14641 gccgcggcga ccttgacgag gatctcgtgg ggcctgggat cgggctttgc ccgctcggcg
    14701 ggccgcagga cttcggggcc gcccggcttc tcgatttcga cggcacgcat ggggaggcgc
    14761 atcttaccgc aggcgccgcg caggactatg atgcggcacg tcatggcgcg cgccctgatc
    14821 acggcatgcc tgtttatcct cgggtccgcg gcggcggcgc acgaggcggc accgctgccc
    14881 ggcgccgacg ggcgcgtggc gctcggcgcc catgtcgagg cgctggagga tcccggcggc
    14941 gcgctgcgcc tgccggacgt gcgcggcgcg cagcacgccg cgcgcttcgc gcgcctggac
    15001 gacggcgggg tgaatttcgg ctacacgcgc tcggcctggt ggctgcgctt cgccctgccg
    15061 ggcggcgctc cggcaggcga ggagctgctg ctcgaagtcg cctaccccag cctggaccgc
    15121 gtcgagttcc acctgccgga ggcgctgccc gggggcgagg cgcgctactg ggcgcgcgtc
    15181 gcgggcgacc agtttccctg ggcggcgcgc gaaatcccgc accgcaacta cctgttccgc
    15241 ttcgccgcac ccgcggccgc gggcgcgcac tattactacc tgcgcgtcga gtcgcgcagc
    15301 gtgctgaccg tgccgctcac gctgtggcgc cccgaggcct tcgcgcggca cgaccgcgac
    15361 acgcagctgg tgctcggcct gttctacggc ctgggcgccg ccatgctgct gtacaacctg
    15421 atgctgttct tcgcgctgcg cgaccgcatg tacctgtatt acgtgctcta cgtcgcggcg
    15481 ttcggcctgt tcctgttcgg cttcaacggc ctcgcctacc agtatctgtg gccgcaaagc
    15541 ccctggggcg ccaacaacct ggtggccgcc acgctggcgc tcgccctcgc gctgggcgcg
    15601 cagttcgcgc gcgccttcct cgactcgcgc cgcatcgcgc cgctcgccga ccgcgcgatg
    15661 ctggcgatcg tggtggcggg cgcggtgctc gccggctgcg ccgccaccgg ctgggggctc
    15721 gactacggcg gcgtgctgcg cgtggtgacg gtgctcggcc tggccacggc ggccgcggcc
    15781 acggggatcg cgctggccgc gctgcgcgag ggctaccggc cggcgcgcta cttcctcctc
    15841 gcctgggcgg cgctgctggc gttcatcgcg ctgggggcgc tgcgcaactt cgcgctggtg
    15901 ccgaccaatt tcctcactgt ctacggcatg cacatcggct tcgcgctcga cgtgctgctg
    15961 ctgtccttcg cgctcggcga ccgcatccgc atgctgcgcc gcgagaacgt ggcggcgcat
    16021 gccgaggcgc tggagtcgca gcagctgctg ctcgaggcga cgcgcgaaag cgagcgcgcg
    16081 ctcgagacgc gcatcgcggt gcgcaccgcg gagctgaacc gcgccaacga gcgcctgcgc
    16141 gccgaggccg ccgatcgcga gcagttgatg gaacagctgc agaagcagga ggagcacctg
    16201 cgcttcatgg cgcagcacga cccgctgacc gggctgccca accgcacctc gatgcagcag
    16261 cgcctggcgc tcgccatgga actcgccaag cgcaaccgca agaaggtggc ggtgatgatg
    16321 gtggacctgg acgacttcaa gcgcatcaac gacacgcgcg gccacccggc cggcgaccag
    16381 gcgctggtgc agctcgccgc gcgcctgcgc accagcgtgc gcggctcgga caccgtggcg
    16441 cgctatggcg gcgacgagtt cgtgatcctg gcgggggagc tcgaccgcgc cgcggacgcg
    16501 gccatggtgg cggagaaggt ggccgacatg gtgagcgtgc cgctccccgt gcagggcggc
    16561 ctggagaagg tctcctgctc catcggcatc agcgtgttcc cggacgacgc cgaggagggc
    16621 gaggcgctga tcgcgctcgc cgacaaggcg atgtacggct cgaaggcggc gcagggccgg
    16681 cgctacgcgt tcttcggcgc ggcctagcgc gcgagctggc gcgccgccca ggcgagatcc
    16741 acgttgccgc ccgagatcac cacgccgacc tttttcccct tggcatcgat cttcccctgc
    16801 agcagcgcgg ccgccgcgag cgcgccggtg ggctcgacca cgatcttcat ccgctcccag
    16861 agatagaaca tccagcgcag cagttcctcg tcggtcacgg tgaggaagtc gctcacgttc
    16921 gcgcggatga tggagaaggt gagcttgccg agggacatgg tgcgcgcccc gtccgcgatg
    16981 gtcatcggca gcggcacgct ttccagccgg ccggcgcgga acgagcgcag gccgtcgtcg
    17041 ccggcggccg gctcgacgcc gatcacgcgg cacctcgggg aaaggtgctg cgccgcgatg
    17101 gcgcagcccg acagcagccc gccgccgccg cagggcacca gcaggtagtc cagcggcccc
    17161 gtgtcctcga tcagttcctt cgccgccgtg ccctgtccgg cgaggatgtg cgggtggtcg
    17221 aacggcggga tcagcgtgag gccgcgctcc gcggcgagct gctcggcgat cttcccccgg
    17281 tcctcgttgc ccgcgtcgta caggaccacc tcggcgccgt agccgcgcgt cgcggcgagc
    17341 ttcaccggcg gcgcgtcggt cggcatgacg atgaccgcgg gcacgcccag cagcttgccg
    17401 gagagcgcca ccgcctgcgc atggttgccc gaggagaagg cgaccacgcc cttgcgcttc
    17461 tgctccggcg acagctgcgc gagcgcgttg tagccgccgc ggaacttgaa ggcgcccatg
    17521 cgctggaagt tctcgcactt gaaatgcacc tcggcgccgg tgagggcgtc gatggtcgcc
    17581 gaggagagga ccggcgtgcg ccgcgcataa gggcggatcc gctcgtgggc ggctgcaatg
    17641 tcgtcgaaag tcacttccat cgttgcagca cctctctggc gacatcgtcg ccccactcct
    17701 gcaggaaatg cccgccctcg gcatggacgt agggctcggg gcaatcgcgg atgacccggc
    17761 gcaaggcgtg catcaccggc gggccgagca ccgggtccct ggcgccgatc gccatgaagg
    17821 attcgccctt ccacgcatgc atccaccagt cgcgcgcctg gcgcgagagc gcggcgccgg
    17881 gcgcatccgg ccggtcgggc accaggttcg ggaaggcgcg cgcgcccgcc ttcgacgccg
    17941 agtcggggta gggcgcctcg tacgcggcgg cttccgcctc gctgaggtgc ctgcaggagc
    18001 gttgcatcag cttgtcgcag ggaaggtcgg gtgtccggct cacgtaagcg cgccaggcga
    18061 ggaagccctc cgacaacggc gcatcgcccg tgccgagcgt ggtgttcatc agcagcactc
    18121 gctcgaagcg ctcgggcatt tccatcggca gggtgagtcc cagcaagccg ccccaatcct
    18181 gcaccaccag ggcgatgcgc gtcaggtcca gccgctcgat gaaatcgagc aggaatgtgc
    18241 ggtgcatgtc gaaggtatag aaagattcct ctgccggctt gtccgagcga ccgaagccga
    18301 ggaagtccgg cgccaccacg cgcgcgccgc ccgccgcgaa gggcggaatc atgcggcggt
    18361 acaggtagct ccaggtcggc tcgccgtgca ggcacagcca ggttcgctcg ccccggcccg
    18421 cttcgacgta gtgggcgcgc aggccgcgcc actcccagta gcgcggcgca tgcgcaaagc
    18481 cgggcaggtc cgcgaaccgc gcgtcggggg tgcgcatgac ccgattgtag aatgtcccgc
    18541 atggactatt ccaagtacca ggacctgaat tgccggctgc tcgagcccgg catcctcgag
    18601 atcgtgatgg gcagggacga cggcaggctg tccaccgcgg gacaccgcat gcacgccgag
    18661 ctggcggaaa tctggcgcga cgtggaccgc gaccccgcgg tgcgcgtcgc catcctgcgc
    18721 ggcgcgggca agggcttttc cgcgggcggg gacttcagcc tgatcgacga catcatccgc
    18781 gacttcgaca cccgcgcgcg cgtctggcgc gaggcgcgcg acctggtcta caacctgatc
    18841 aactgctcca agatcgtggt ctcggcgatg cacggcccgg cggtgggcgc cggcctggtc
    18901 gcggggctgc tcgccgacat ctccatcgcg gcgaagaacg cgcgcatcat cgacggccac
    18961 acgcgcctgg gcgtggcggc gggcgaccac gcggcgatcg tgtggcccct cctgtgcggc
    19021 atggcgaggg ccaagtacta cctgctcacc tgcgagcagc tgtcgggcga ggaggcggag
    19081 aggatcggcc tggtgtcgct gtgctgcgag gaggcgcagc tgcaggcgaa ggcgctcgag
    19141 gtggcgcgca agctcgccgc cggcgcgcag accgcgctgc gctggaccaa ggccagcctg
    19201 aacaactggc tgcgcatggc gggcccggcg ttcgacaact ccctggcgct ggagatgctc
    19261 ggcttttccg gccccgaggc gaaggaaggc gccgcctcgc accgcgagaa gaggaagccg
    19321 gagtttccgc cggcgccgaa gggcttctag gccttcggcg cgagcaggcg ctcgaggtcc
    19381 ttcgccaccg ccgccgggtc gggcggcacg cgcccctgcg tggtgagcag cacgcgcacc
    19441 ggcaggtcca ccagcgaggg ctccggatgg tcgcgcaggt gcgccgcatt ctgcagcatc
    19501 ggcacgatgc tctgcgtctt gatgcccgcc atcgcgctgt cgatgccctg gatgaccgcg
    19561 gtctgcgcgc gggcgatgcc gatgagcagt tctgcgagct gttgcgcgga gatttccatg
    19621 ccgttctccc ccttggcgga tgtcgcgccg atggtagcat ttgccctcga tgctgcgccg
    19681 ctcgctcgcc gcggtactgc tgttggttct gggcgcggcc gcgggcctcg cgctgcacct
    19741 cgccaccgcc aggggagaca ccatgttcgc cggcaagcgc ccgggttacc ttggcgtgaa
    19801 ggacgggcgc ctcgcgcgcc cgaagagcac gcccaactgc gtcgccagcc aggccgatgc
    19861 cgcggatggg gagcactatg tcgcgccgat ccgtttcaag ggcagcgcgc tggaggccat
    19921 cgccgcggcg cgcaaggcgg tggaaagcat ggcccgcgcc accgtcgtgc gccacgaggg
    19981 caactacctg tacgccgaat tcaagtcgag gctgatgggg ttcgtggacg acgtcgagtt
    20041 cacctacgac gagaaggcag gggtgctgca cctgcgctcg gcctcgcgcc tcgggcggcg
    20101 cgacttcggg gtcaaccgcg cgcgcgcgga ggcattgcgc gcgcgcatcg aaggccgcat
    20161 caatacctga gggaggtgcc gatggccgcc tttccgcgca tgttgctcct cgtgctggcg
    20221 ctcggcgcgg cgccggccca cgcccaggac aagccgaagg acgacgccga cgagctgctc
    20281 gcggcggtgg tgcaggtgaa ggcgaagatc ctgcccgacg cgcgcagcct cgccacgctg
    20341 gggccgcagc gccagggcag cggcgtgctg gtgcgcgagg gctacgtgct caccatcggc
    20401 tacctggtga tcgaggccga ggcgatcgag gtctccggcg ccggcggcaa gaccgtgccg
    20461 gcgacgctcg ccgcctacga ccacgccagc ggcttcggcc tgctgcgcct cgcggcgccg
    20521 ctcgccgcca ggccgctgcc gctgggcgac gcgagcgcgc tcgccgagcg cgaaccggcg
    20581 atgatcgccg cccacggcgg ggccgaaggc ctcaacctgg tgtacgtcgt atcgcgccgg
    20641 ccgttctccg gcagctggga gtaccagctc gacgccgcga tctacaccta cccggcggtg
    20701 cagaactggt cgggggccgc gctgatcggc cccaagggcg acctgctcgg catcggctcg
    20761 ctcatcgtgc ccgacgcggg cggcccgggc acgcaatccc ccggcaacct gttcgtgccg
    20821 atcgacctgc tcaagccgat cctggaagac ctgatcgcca agggccgcag cgccgggccg
    20881 cagcggccct ggctgggcat ctatgccgag gagctgcgcg gccggctgtt cgtgcaacgc
    20941 gtggcgcccg agggcccggc ggagcgcgcc gggctgaagg cgggcgacct ggtgctcggc
    21001 gtgggcggcg aggcggtcgg ctcgctcgcc gagttctacc gcaagctgtg ggcgcggggc
    21061 gccgccggcg tggaggtgcc cctgcgcgtg ctgcaggggc tgcagctgaa ggagctgagc
    21121 gtgcgctcga tcgaccgcct ggagtactac cggcgcaagc cgagctactg agcggcgagg
    21181 gccggcgccg cgggcggcgc gcgccgcagc agctcgtcgc gtcccgagtc cgccaccagg
    21241 cgcgcgtggc gcacgttctt gtggatctcc tccaggtaca ggttctggcc gccgaaatgg
    21301 cgcgcggcgt attcgtgcgc gcgcgccagc tgcaggtcga ggcgctgggc ggcgttgtgg
    21361 aagaaggccg gcgtcctgcg caccagctcc agcgccgagc ggtaaagcgc gcgcgccacg
    21421 ccgcccgcga cgaactccgg gtacaggcgg tcgcggcatt tctccaggta gcaccggtcc
    21481 gacatctgcg ccaggatatc cgcgctgccg agcatctgcc cgatgcggcg cagcaccgga
    21541 tggtcgacgc ggatcgattc ggccgagcgc tcgtagcccg tgtagtgcag cagcggcgcc
    21601 gcgctcgcgg cgtagcgcgc cagtccggcg ccgcgcaggt agccggcgac gaagccggcg
    21661 ccgcgcgatg cgcgcctgag cgtgtactcc gcgccgttgc cgtgccggcg gtcggcgcgc
    21721 cggcgcaggt agcccacgtc gtgcagcagc gcgcagatca cgccgaccgt gaacagcggg
    21781 gccggcagcg gcggcgggcc gctgcgcgag cgctcgtagc cgtccatcag ccgcgcctgc
    21841 gccagcgtca cgtcgagcac gtgctgcagg tcgtggtagg gcgtgtcgca ggcatggaaa
    21901 cgcgggtgcg cgccgcggta caggcgcacc gtgtcggcga aggcgcgctc gatcggccgc
    21961 ggcgaggcct gcggatacag gtcctggaac aggcgcgcga cctcggcgcc gaccgcggcg
    22021 ggttcggtgg tgcggatcct gccggaaacg tcgtggtcgt tgcggcgcat gcaggcgccc
    22081 cctatgcgct cgctggaaga ggcgctagga tgcgcagcag agggacagcg cagcggtgaa
    22141 ctttgtcacg accggcgggg agcatccgca agaatttgtg cggccccggc gcgaggcgcg
    22201 cggtgagtaa cctgccgctc accgaggagg agcgcgcgca gatcgaggcg cgcatccacg
    22261 ccctggaggt cgagcacagc gacctcgacg acgtcatcgc ccacctggtg gaagtcccgg
    22321 tgcaggacca gctgcagctg cgccggctga agaagaggaa gctcctgctg aaggacctga
    22381 tcgcgcgcct gcgcgaccgc ctgatccccg acatcatcgc ttgacgcgcg cacgccgcgg
    22441 cggcggcgcg tctagcgttc tgaaatccgc cggcgcgacg atctcgatca gctcgaggtc
    22501 gcgcgagtgg ctgatctcgc ggtggcggat gcccggcggc tggtagaagc agctgccggc
    22561 cttcatgcgc accttgccca cgccttcgta ctcgaacacc gcccagccct tgagcacgta
    22621 cacgaactgc acctcgcaca ggtgccggtg ccactggccg tgcggcgcct tgccggggcg
    22681 ggcgcggatc acgtgcgcca ccaccttgcc gcgcgtggcg cgcttgatgc cgaggtcgcg
    22741 gtactcgaaa tagttgcgca ggccgcggcg cacgaacctc gacctggcgc ggtggctcac
    22801 tgcgaatcgg ctggccatgg ctctccccgg gcggtggcga ttcgggctat ccttgcgcca
    22861 cccatgttcg accaattcat cggcgtcaag ccggtcgagg aacggcatcg cttcgacgtc
    22921 ggccgcctgg cgcggtacct gcgcggcaag atagacgatt ggcagggcga actcaaggtc
    22981 gagcagttca ggggcgggca gtccaacccg acctaccgcg tcaccgcggg cgcgaaacgc
    23041 tacgcgctgc gccgcaagcc cccgggcaag ctcctgccct cggcgcacgc cgtggaccgc
    23101 gagtacaagg tgatcaaggc gctgcacggc acgggcttcc cggtggcgag gccgtacctg
    23161 ctgtgcgagg acgaggccgt ggtcggcacg gcgttctacg tgatggactg cgtcgaaggc
    23221 cgcgtgctgt gggaccaggc gctgcccggc atgaccccgg ccgcgcgcgg cgcgatctgg
    23281 gacgagctga accgcgtcat cgcgctgctg cacggcatcg actggaaggc cgtcgggctg
    23341 gaggatttcg gcaagccggg cgactacatc gcgcgccagg tgtcgcgctg gaccaagcag
    23401 taccaggcct cggagctgga gaagatcgag gagatggacc gcctcatcgc gtggctgccg
    23461 cagcacatcc cggcgggcga cgagaccgcg gtggtgcacg gcgattttcg gctggacaac
    23521 accgtgttcc acccgagcga gccgcgcatc ctcgcggtgc tcgactggga gctctccacg
    23581 ctcgggcacc cgctggcgga cttcgcctac cactgcatga gctggcacat cgcgccgggg
    23641 aagttccgcg gcatcgctgg cctggacctc gccgcgctcg gcatcccggg cgagcgcgag
    23701 tacgtcgagc gctactgcag gcgcaccggg cgggcgggca tcgacccctc gacctgggac
    23761 tactacatgg cctacaacct gttccgcatc gccggcatca cgcagggcat cgccaagcgc
    23821 gtggtggacg gcaccgcggc gagcgagtac gcgcgcgagg ccggctcgcg cacgcggccg
    23881 ctcgccgagc tgggatgggc gcaggtggag aagattctgc aaagggcagc ctaggagagg
    23941 gaacatggat ttcgaatatt cgccgcgggt gaaggaaatg cagcagcgcc tcaaggcgtt
    24001 catggccgag cacatctatc cgagcgagcc ggcctactac gcgcacacgc gcagcgacag
    24061 gcgctgggag ccggtgccgg tgatcgagaa gttgaagcca ctggcgcgca aggccgggct
    24121 gtggaacctg ttcctgccgc attccaagcg cgcgccggag ggcctgtcga acctcgagta
    24181 cgcgccgctg tgcgagatca tgggccgcac cagctggtcg cccgaggtgt tcaactgctc
    24241 ggcgcccgac acgggcaaca tggaaaccat cgagcgctac ggcaccgagg agcagaagaa
    24301 gcagtggctc gagccgctgc tgcgcggcga gatccgctcg gcgttcctga tgaccgagcc
    24361 ggcggtggcg agctcggacg ccaccaacat ccagatgcag atcaggcgcg acggcaacga
    24421 ctacgtcatc aacgggcgca agtggtggtc ctccggcgcg ggcgattcgc gctgcaaggt
    24481 gtacatcgtg atgggcaaga ccaacccgga tgcagcgtcg cgccacagcc agcagtcgat
    24541 gatcctggtg ccggccggcg ccaagggcat caccatcctg caccacctga acgtcttcgg
    24601 ctacgacgac gcgccgcacg gccacatgga gatcgagttc aaggacgtgc gcgtgcccgc
    24661 gtcgaacatc ctgctcggcg agggccgcgg cttcgagatc gcgcaggggc gcctggggcc
    24721 ggggcgcatc caccactgca tgcgcctcat cggccaggcc gagcgcgccc tcgagaagat
    24781 gtgcaagcgc gccaccgagc gcgtcgcctt cggccgcacg gtggccgagc agggcgtgac
    24841 gcgcgagcgc atcgcggaag cccgcatcct catcgacagc acgcgcctga tggtgctgca
    24901 cgcggcctgg cgcatggaca cggtcggcaa caa
//
LOCUS       DASRIE010000054         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2930001, whole genome shotgun sequence.
ACCESSION   DASRIE010000054 DASRIE010000000
VERSION     DASRIE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2930001"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /gene="phaC"
                     /locus_tag="VFB53_02585"
     CDS             complement(<1..1660)
                     /gene="phaC"
                     /locus_tag="VFB53_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZN85649.1"
                     /translation="MSTPPSPENFVASIAQSGNDMAKNWLRMAASAPGQPLPADYLAR
                     LGADGARLGALQAEYLDRQLRLWTGLSSGKTGAEARPEAGDRRFAAKEWRDNPYYDYL
                     KQSYLLAAAFLEQLVEAAALEGQAKERLRFTVRQWVDAMSPANFAATNPEVLRLAQET
                     RGESLARGLANLLADAGKGHISQTDESAFEVGRNLAVTPGQVIFENELMQLIQYAPAT
                     AEVARRPLVMVPPCINKFYILDLQPENSFVRYAVGEGHTVFMVSWRNVGPGQGRFGWD
                     DYLEMGVLSALRTAREVAKSDRVNALGFCVGGTLLGAALAVLAARGEALVESATYLAT
                     MLDFSDTGQIGLFVDEASVAQREAALGAGGILPARELSTVFSSLRANDLIWPYVVNNY
                     LKGGTPAAFDLLYWNADGTNLPGPMYCWYLRNTYLENRLRQPGALRCLGVPLDLSKVD
                     RPSFIVATREDHIVPWRSAYRSRGLLGGEKRFVLGASGHVAGIINPAAKNRRSYWDGE
                     GAPQDAEAWLAQARETQGSWWPQWAAWLERHGGGARKAPKSAGNA"
     gene            complement(1715..2476)
                     /locus_tag="VFB53_02590"
     CDS             complement(1715..2476)
                     /locus_tag="VFB53_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZN85650.1"
                     /translation="MTLSWIVLASLAGGLLSVGAAAIALVLRASWVPMLVSFAIGTLL
                     GAAFLEVVPHAFESGDAHQAAGAILGGILGFFLLEKLLLWRHSHDHGEGALPHHHHEH
                     SRAGLMIVIGDTVHNFVDGILIAAAFLQSPELGLITAVAIVAHEIPQEVGDFLVLLNS
                     GYSRSRAFAMNLLSSCGTLVGGVLGYFTLAGVAHWEPTLLGIVAASMIYVAVADLIPG
                     LHKRPELRATAQQALLIAAGVGAIALVRMVAGHGH"
     gene            complement(2473..3114)
                     /gene="pgeF"
                     /locus_tag="VFB53_02595"
     CDS             complement(2473..3114)
                     /gene="pgeF"
                     /locus_tag="VFB53_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZN85651.1"
                     /translation="MIVPDWPAPARVRALVSTRGEADPALPAPPAWLRQVHGTAVVDA
                     AQAAMEPEADASVARVPGVVCAVKSADCLPVLLADESGGVVGAAHAGWRGLAAGVLEA
                     TVDAMQVPPRTLLAWLGPAIGPKVYEVGEEVRAAFLARDARAESAFVPNRPGHWLLDL
                     YAVARQRLAARGVTRVHGGGYCTFSDAARFPSYRRERTRERIAAFVWLESRSR"
     gene            complement(3111..4061)
                     /locus_tag="VFB53_02600"
     CDS             complement(3111..4061)
                     /locus_tag="VFB53_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN85652.1"
                     /translation="MGRDYSDDGNADWDASFVAPMRAEVPRSFGGLRLDQALARVFPQ
                     YSRNRLQAWLAAGHIRIDGARGESRQAVRGGERVELAPPAAPAVAPRAQKMRLAIVHE
                     DADLIVLDKPAGLVVHPGAGVPDGTLMNGLLAHCPGLAGVPRAGIVHRLDKDTTGLLV
                     VAKTVEAQASLARQLESRSMRRVYLAVVQGDPPSSGVIDAPLGRDTRARTKMAVTQRG
                     KDAVTRYRVLERYGHAALVECRLESGRTHQIRVHFQHIRHPLVGEGTYRRGTRHGVAF
                     GRQALHAVELSLVHPRSGKTMTWQAEPPADFQKLLKSLRR"
     gene            4060..>4374
                     /gene="bamD"
                     /locus_tag="VFB53_02605"
     CDS             4060..>4374
                     /gene="bamD"
                     /locus_tag="VFB53_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZN85653.1"
                     /translation="MRTSVLALAAAAVLSLAGCGSTAEPPDETAGWSAQRLYGEAQDA
                     MAQRDWAKVIQYLQKLEARYPYGRYAQQAQLEIAYAYWKDNERASAIAATDRFIKLHP
                     NHD"
BASE COUNT          622 a         1615 c         1550 g          587 t
ORIGIN      
        1 gcgcattgcc ggcgctcttc ggcgccttgc gcgcgccgcc gccgtgccgc tccagccagg
       61 ccgcccattg cggccaccag ctgccctgcg tttcccgcgc ctgcgcgagc caggcctccg
      121 cgtcctgcgg tgcgccttcg ccgtcccagt agctgcggcg gttcttcgcc gccgggttga
      181 tgatgcccgc gacatgcccg ctcgcgccca gcacgaagcg cttctcgccg ccgagcaggc
      241 cgcgcgagcg gtaggcggag cgccagggca cgatgtggtc ctcgcgcgtc gccacgatga
      301 agctggggcg gtccaccttg gagagatcga gcggcacgcc gaggcagcgg agcgcccccg
      361 gctggcgcag cctgttctcg agataggtgt tgcgcaggta ccagcagtac atcgggcccg
      421 gcaggttggt gccgtcggcg ttccagtaca gcaggtcgaa ggccgccggc gtgccgccct
      481 tgaggtagtt gttcaccacg tagggccaga tcaggtcgtt ggcgcgcagg ctcgagaaca
      541 cggtggacag ctcgcgcgcg ggcaggatgc ccccggcccc gagcgccgcc tcgcgctgcg
      601 cgacgctcgc ctcgtcgacg aacaggccga tctggccggt gtcggagaaa tcgagcatcg
      661 tcgcgaggta ggtggcgctc tccaccaggg cttcgcccct cgccgcgagc acggcgagcg
      721 ccgcgccgag cagcgtgccg ccgacgcaga acccgagcgc gttgacgcgg tcgctcttcg
      781 cgacctcgcg cgccgtgcgc agcgcgctca gcacgcccat ttccaggtag tcgtcccagc
      841 cgaagcggcc ctgccccggc ccgacgttgc gccaggaaac catgaacacc gtatgcccct
      901 cgcccaccgc gtagcgcacg aaggaatttt ccggctgcag gtcgaggatg tagaacttgt
      961 tgatgcaggg cggcaccatc accaggggac gcctggccac ttccgcggtg gcgggcgcgt
     1021 actggatgag ctgcatcagc tcgttctcga agatcacctg gcccggcgtg acggcgaggt
     1081 tgcggcccac ctcgaacgcg ctctcgtcag tctggctgat gtgccccttg ccggcgtcgg
     1141 cgagcaggtt ggcgaggccg cgcgccaggc tctcgccgcg cgtttcctgc gccagacgca
     1201 gcacctcggg gttggtggcg gcgaaattcg ccgggctcat cgcgtccacc cattggcgca
     1261 ccgtgaagcg caggcgctct ttcgcctggc cctcgagcgc ggccgcctcc accagctgct
     1321 ccaggaaagc ggcggccagg aggtaggact gcttcaggta gtcgtagtac gggttatcgc
     1381 gccactcctt ggcggcgaag cgccggtcgc ccgcttccgg gcgcgcctcc gccccggtct
     1441 tgccggacga cagcccggtc cacagccgca gctgcctgtc gaggtattcg gcctgcagcg
     1501 cgccgagccg cgcgccgtcg gcgccgaggc gcgcgaggta gtccgcgggc agcggctggc
     1561 cgggggcgct ggccgccatg cgcagccagt tcttcgccat gtcgttgccg gactgggcga
     1621 tcgacgcgac gaagttctcc ggggacggtg gggtgctcaa ggcggcgtat tttgcgccgc
     1681 acaaactggc cggtcaaaca ccccggcggg tttttcagtg gccgtggccg gccaccatcc
     1741 gcaccagcgc aatcgcgccg acccccgccg cgatgagcag ggcctgctgc gccgtggcgc
     1801 gcagctcggg ccgcttgtgc aggcccggaa tcaggtcggc caccgcgacg tagatcatgc
     1861 tcgcggcgac gatgcccagc agggtcggtt cccagtgcgc gacgcccgcc agcgtgaagt
     1921 agcccagcac gccgcccacc agcgtgccgc aggacgagag gaggttcatg gcgaaggcgc
     1981 gcgagcgcga gtagcccgag ttgagcagca ccaggaagtc gccgacttcc tgcgggatct
     2041 cgtgcgcgac gatggccacg gcggtgatca ggcccagttc cgggctctgc aggaaggcgg
     2101 cggcgatgag gatgccgtcg acgaaattgt gcaccgtgtc gccgatcacg atcatcaggc
     2161 cggcgcgcga gtgctcgtgg tggtggtgcg gcaacgcgcc ctcgccgtgg tcgtgcgagt
     2221 ggcgccacag caggagcttt tccagcagga aaaagccgag gatgccgccg aggatcgcgc
     2281 ccgccgcctg gtgcgcgtcg cccgactcga aggcgtgcgg caccacctcg aggaaggccg
     2341 cgccgagcag cgtgccgatg gcgaaggaca ccagcatcgg cacccaggag gcgcgcagca
     2401 ccagcgcgat cgcggcggcg cccaccgaca gcagcccgcc ggcgaggctg gcgagaacaa
     2461 tccagctcag agtcatcggg agcgggattc taaccagacg aaggccgcga tgcgctcgcg
     2521 cgtgcgttcg cgccggtacg agggaaaacg cgccgcgtcg gaaaaggtgc agtagccccc
     2581 gccgtggacg cgcgtcacgc cgcgcgcggc gaggcgctgg cgcgccaccg cgtagaggtc
     2641 gagcagccag tgcccggggc ggttcggcac gaaggcgctc tcggcgcgcg cgtcgcgcgc
     2701 gagaaacgcc gcacgcacct cctcgcccac ctcgtacacc ttggggccga tcgccggccc
     2761 gagccaggcg agcagcgtgc gcggcggaac ctgcatcgcg tcgacggtcg cctcgagcac
     2821 gcccgcggcg aggccgcgcc agccggcgtg cgccgcgccg accacgccgc ccgactcgtc
     2881 ggcgagcagc accggcaggc agtcggcgga cttcaccgcg cagaccacgc cgggcacgcg
     2941 cgccaccgac gcatcggcct cgggctccat cgcagcctgc gccgcgtcga ccaccgcggt
     3001 gccgtgcacc tggcgcagcc atgccggcgg cgccggcagc gccgggtcgg cttcgccgcg
     3061 cgtgctcacc agcgcgcgca cgcgcgccgg cgccggccag tcgggcacga tcatcggcgc
     3121 aggctcttca gcagcttctg gaaatcggcc ggcggctccg cctgccaggt catggtcttg
     3181 ccggagcgcg gatgcaccag cgacagctcc accgcgtgca gcgcctggcg gccgaaggcg
     3241 acgccatggc gcgtgccgcg ccggtaggtg ccttcgccca ccagcgggtg gcggatgtgc
     3301 tggaaatgca cccggatctg gtgcgtgcgg ccgctttcca ggcggcattc gaccagcgcc
     3361 gcatggccgt agcgctcgag cacgcggtag cgcgtgaccg cgtccttgcc gcgctgcgtg
     3421 accgccatct tggtgcgcgc gcgcgtgtcg cggcccagcg gcgcgtcgat caccccgctc
     3481 gagggcgggt cgccctgcac caccgccagg tacacgcggc gcatgctgcg gctctccagc
     3541 tggcgcgcga ggctggcctg cgcttcgacc gtcttcgcca ccaccagcag gcccgtggtg
     3601 tccttgtcca ggcggtgcac gatccccgcg cgcggcacgc cggcgagccc cgggcagtgc
     3661 gcgagcagcc cgttcatcag cgtgccgtcg ggcacgcccg cgcccgggtg caccaccagc
     3721 ccggcgggct tgtcgaggac gatcaggtcg gcgtcctcgt gcacgatggc gagacgcatc
     3781 ttctgcgcgc gcggcgcgac tgccggcgcc gccggcggcg ccagctcgac gcgctcgccg
     3841 ccgcgcaccg cctgccgcga ctcgccgcgc gccccgtcga tgcggatgtg gcccgccgcc
     3901 agccaggcct gcaggcggtt gcgcgaatac tgcgggaaca cgcgcgccag cgcctggtcc
     3961 aagcgcagtc cgccgaacga tcgcggcact tcggcgcgca tcggcgcgac gaaggacgca
     4021 tcccagtcgg cattcccgtc gtcgctataa tcgcggccca tgcgaactag tgtacttgct
     4081 ctcgccgccg ccgccgtgct gtccttggcc ggctgcggct cgaccgcgga gccgcccgac
     4141 gagaccgccg gctggtcggc gcagcgcctg tacggcgagg cgcaggacgc catggcgcag
     4201 cgcgactggg cgaaggtgat ccagtacctg cagaagctcg aggcgcgcta cccctacggc
     4261 cgctacgcgc agcaggcgca gctcgagatc gcctacgcct actggaagga caacgagcgc
     4321 gcctcggcca tcgccgccac cgaccgcttc atcaagctgc atcccaacca cgac
//
LOCUS       DASRIE010000055         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_829366, whole genome shotgun sequence.
ACCESSION   DASRIE010000055 DASRIE010000000
VERSION     DASRIE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829366"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFB53_02610"
     CDS             <1..754
                     /locus_tag="VFB53_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN85654.1"
                     /translation="IILVTGPTGSGKTTTLYSILNHINSDGVNIMTLEDPVEYPMTLI
                     RQTSVAEAAKLDFANGIRALMRQDPDIILVGEVRDHETAEMAFRAAMTGHQVYTTLHT
                     NSAVGAFPRLLDIGIVPDILAGNIIGILAQRLVRKLCRTCRQSYDPDVKTRRLLGYSP
                     TEPAALFRAVGCERCEYQGYSGRQSILEILKIDPGTDELIARRATAREILTAARAGGF
                     KTLADDGVRLVRAGVTALDELMRVVDLTDRMA"
     gene            765..1970
                     /locus_tag="VFB53_02615"
     CDS             765..1970
                     /locus_tag="VFB53_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN85655.1"
                     /translation="MALYTYKAIDLQGKRVLGRLEALNLFDLEQRLARMELDLVAGAP
                     ASDMSRLLGGGRVSRQDLINFCFHLEQLATAGVPIMEGLVDLRDSVENPRFREVVSGL
                     IEAIQGGHNLSQALADYPSVFSKVFVSLVRSGEQTGKLADVLKALAESLKWEDELAAQ
                     TKKMMMYPAFVGSIVLVVTFFLMIYLVPQMTGFIRNMGQEIPLQTVILMAVSAFFVDY
                     WWAIAALPFVIWGGLKFGIATNPAIAYAVDNYKISMPLLGPILRKIYLSRFASSFALM
                     YGSGITVLDAIRSCEEIVGNLPLERALRRAGQQIAEGKNLTAAFQDLGVFPPLVIRML
                     RIGEGTGKLDTALLNVSYFYNREVREQISRVQAMIEPALTLVLGALLGWVMLSVLGPV
                     YDSISKMKF"
     gene            1975..3519
                     /locus_tag="VFB53_02620"
     CDS             1975..3519
                     /locus_tag="VFB53_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85656.1"
                     /translation="MAARYLLYFTGEDHYLYAAAAGRLELEAKFAGDDLGVGAFREHL
                     RGRRGALFAVVADLAGEDFHEEQIPSLRGADRDAVVQRRLAQRYRDTRLAAALSLGAA
                     TTAQRRNERLLLASFTNTQQFTPWLDALEEAGVKLAGVYSVPLLAPALAARLGARGER
                     ALLVSANSAGLRQCFVENGRLRFARLERTVDMVPQALAAFARSETLRLVQYLGTLRAL
                     PREGAPVPALVIAPTGQRAVFEQALVSDARVTFQTVDAAEAARAVGLRQAPEGTAAEA
                     LYLHLAAHKAPREQFASRSDRRSYSIWQLQRGIAAAGAAALVACALFGGARWLQIWEV
                     RALAAAQGEEARRAASQYERITATFPVTQTSTENLKVAVLEFQRIAERFPPPDGALVH
                     VSKVLAQFPQVEIDAVTWNSGERRESRSAAPGAAAPAVKPGDGALQLELSGRVNATQR
                     TDYRSITAQVQRLAQAMASSGYELVRTQLPFDITSEGVLTGDIAGGGDSGEAPRFTIV
                     IARRLP"
     gene            3516..4124
                     /locus_tag="VFB53_02625"
     CDS             3516..4124
                     /locus_tag="VFB53_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85657.1"
                     /translation="MTFTREELRKLTIPALVALALLGAGIAFVWSGNAALADANRSLA
                     GAQAERQKATERLARISEEEREVSQKLDIYRRLQALNILGEERRLEWADAIARIRKQR
                     ELLDLSYRVDKQRLLLSAPGNPGNVDLYASNMRVQLALLHEGDLLRFLADLRESGNAY
                     YGVRRCALTRTGQAATGASIVPRLRAECDIELVTMIDRAAKR"
     gene            4175..4546
                     /locus_tag="VFB53_02630"
     CDS             4175..4546
                     /locus_tag="VFB53_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85658.1"
                     /translation="MAQEPLGRLFFTPEQRDALDARRKARVPDRPTAPVAASPTTRFD
                     GYVQRSGGRSTVFVNGEPVTEGSSDAPSVDTGRKDGRVPINVGEGGGRRALKPGEVLD
                     RGTGDVRDVLGEGEIRVRRSP"
     gene            4543..>5281
                     /locus_tag="VFB53_02635"
     CDS             4543..>5281
                     /locus_tag="VFB53_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85659.1"
                     /translation="MRRLSGRQRRQHGAALILFLTILVLGVAWFAVGALGKAPVATAE
                     REVITGRALQAAKQALLAYAAQYAARSTTADPGQMPCPESLTLANPGEASTSCSATLL
                     VVGRLPWKTLGIDPLRDGAGEPLWYMMRGFRDPPINFGTAGQLTYNGATVVAMIIAPG
                     VPLSTTGTPPAGCTAQNQLVATRNAATLNPANFIECGVATGSIASPGDSAWTNDRAIA
                     ITAAEWADAIAPAIADRLQRQVAPTLEE"
BASE COUNT          803 a         1936 c         1778 g          764 t
ORIGIN      
        1 catcatcctg gtgaccgggc ccaccggcag cggcaagacc accacgctgt actcgatcct
       61 gaatcacatc aactccgacg gcgtcaacat catgacgctg gaagatccgg tcgagtaccc
      121 gatgacgctc atccgtcaaa cgtccgtggc ggaggcggcg aagctcgact tcgccaacgg
      181 catccgcgcg ctgatgcgcc aggaccccga catcatcctg gtcggcgagg tgcgcgacca
      241 cgagaccgcc gaaatggcgt tccgcgccgc catgaccggc caccaggtgt acaccaccct
      301 gcataccaac tcggcggtcg gcgcgtttcc gcgcctgctc gacatcggca tcgtgcccga
      361 catcctcgcc ggcaacatca tcggcatcct cgcccagcgc ctggtgcgca agctctgccg
      421 cacctgccgc cagtcgtatg acccggacgt caagacgcgg cgcctgctgg gctattcgcc
      481 caccgagccg gcggcgctgt tccgcgccgt cggctgcgag cgctgcgagt accagggcta
      541 cagcgggcgc cagagcatcc tcgagatcct gaagatcgac ccgggcaccg acgagctgat
      601 cgcgcggcgc gcgaccgcgc gcgagatcct gaccgcagcg cgcgccggcg gcttcaagac
      661 gctggccgac gacggcgtgc ggctggtgcg cgcgggagtg accgcgctgg acgagctgat
      721 gcgcgtagtc gatctcaccg accgcatggc ctgagacgca ggcgatggcg ctttacacct
      781 acaaggccat cgatctccag ggcaagcgcg tcctcggccg cctggaggcg ctcaacctgt
      841 tcgacctcga gcagcgcctc gcgcgcatgg agctcgacct cgtcgccggg gcgccggcga
      901 gcgacatgtc gcgcctgctg ggcggcggcc gggtgtcgcg ccaggacctg atcaacttct
      961 gcttccacct cgagcagctc gccaccgcgg gggtgccgat catggaaggc ctggtcgacc
     1021 tgcgcgacag cgtggagaac ccgcgctttc gcgaagtcgt ctccggcctg atcgaggcga
     1081 tccagggcgg ccacaacctg tcgcaggcgc tcgccgacta cccgagcgtg ttcagcaagg
     1141 tgttcgtgag cctggtgcgc tcgggcgagc agaccggcaa gctcgccgac gtgctgaagg
     1201 cgctggccga gtcgctgaag tgggaggacg agctcgccgc gcagaccaag aagatgatga
     1261 tgtacccggc cttcgtcggc agcatcgtgc tggtggtgac gttcttcctg atgatctacc
     1321 tcgtgccgca gatgaccggc ttcatccgca acatgggaca ggagatcccg ctgcagacgg
     1381 tgatcctgat ggcggtgtcg gcgttcttcg tcgactactg gtgggcgatc gctgccctgc
     1441 cgttcgtcat ctggggcggg ctgaagttcg gcatcgccac caacccggcg atcgcgtacg
     1501 ccgtcgacaa ctacaagatc tcgatgccgc tgctcggccc gatcctgcgc aagatctacc
     1561 tgtcgcgctt cgcctcgagc ttcgccctga tgtacggctc cggcatcacg gtgctcgacg
     1621 ccatccgcag ctgcgaggag atcgtcggca acctgccgct cgagcgcgcg ctgcgccggg
     1681 cggggcagca gatcgccgag ggcaagaacc tgaccgccgc gttccaggac ctgggggtgt
     1741 tcccgccgct ggtgatccgc atgctgcgca tcggcgaggg caccggcaag ctggataccg
     1801 cgctgctcaa cgtcagctac ttctacaacc gcgaagtgcg cgagcagatc tcccgggtgc
     1861 aggcgatgat cgagccggcg ctgacgctgg tgctcggcgc gctcctcggc tgggtgatgc
     1921 tctcggtgct cggcccggtg tacgactcga tctcgaagat gaaattctga cggcatggcg
     1981 gcgcgctacc tcctgtactt caccggcgag gatcactacc tctacgccgc cgccgccggc
     2041 aggctcgagc tggaagcgaa attcgcgggc gacgacctcg gcgtcggcgc gttccgcgaa
     2101 cacctgcgcg gccggcgcgg cgcgctgttc gcggtagtcg cggacctcgc cggcgaggac
     2161 ttccacgaag agcagatccc gtcgctgcgc ggcgccgatc gcgacgcggt ggtgcagcgc
     2221 cggctcgccc agcgctaccg cgacacgcgc ctcgccgcgg cgctttcgct cggcgcggcg
     2281 accacggcgc agcggcgcaa cgagcgcctg ctcctcgcct cgttcaccaa tacgcagcag
     2341 ttcacgccct ggctcgacgc gctcgaggag gccggggtca agctcgccgg cgtctattcg
     2401 gtgccgctgc tcgcgccggc gctcgccgcc cggctcggtg cgcgcggcga gcgcgccctc
     2461 ctggtcagcg ccaatagcgc cggactgcgc caatgcttcg tcgagaacgg caggctgcgc
     2521 ttcgcccgcc tcgagcgcac cgtcgacatg gtgccgcagg cgctggcggc gttcgcgcgc
     2581 tccgaaacgc tgcggctggt gcaatacctc ggcacgctgc gcgcgctgcc acgcgaaggc
     2641 gcgccggttc cggcgctggt catagcgccg acgggacagc gcgcggtctt cgagcaggcg
     2701 ctggtttcgg acgcgcgcgt caccttccag accgtggacg ccgcggaggc ggcgcgcgcc
     2761 gtcgggctgc gccaggcgcc cgagggcacc gcggcggaag cgctctacct gcacctcgcg
     2821 gcgcacaagg cgccgcgcga gcagttcgcc agccgttcgg accgccgcag ctactccatc
     2881 tggcaactgc agcgcggcat cgccgccgcg ggcgcggcgg cgctcgtcgc ctgcgcgctt
     2941 ttcggcggcg cgcgctggct gcaaatctgg gaagtgcgcg ctctcgccgc cgcgcaaggc
     3001 gaggaggcgc gacgcgccgc cagccagtac gagcgcatca cggcgacgtt cccggtgacg
     3061 cagaccagca ccgagaacct caaggtcgcg gtgctggaat tccagcgcat cgccgagcgc
     3121 ttcccgccgc cggacggcgc gctggtgcac gtgtcgaagg tgctcgcgca gttcccgcag
     3181 gtggagatcg acgccgtcac ctggaactcg ggcgagcgcc gcgagtcgcg atccgcagcg
     3241 ccgggcgcgg ccgcgccggc ggtcaagccc ggcgacggcg cgctgcagct cgagctctcc
     3301 ggacgcgtca acgccacgca acgcaccgac taccgcagca tcaccgcgca ggtccagcgc
     3361 ctcgcccagg cgatggcgag ctcgggctac gagctggtgc gcacgcagct gccgttcgac
     3421 atcacctcgg agggcgtgct gaccggcgat atcgccggcg gcggcgactc cggcgaggcg
     3481 ccgcgcttta ccatcgtcat cgcgcggcgg ctgccatgac gttcacacgc gaggagctgc
     3541 gcaagctcac cattcctgcg ctggtggcgc tcgctctcct cggcgccggc atcgccttcg
     3601 tctggagcgg aaacgcggcg ctcgccgacg cgaaccgcag cctggcgggc gcacaggccg
     3661 agcgccagaa ggcgaccgag cgcctggcgc gcatctcgga agaggagcgc gaggtaagcc
     3721 agaagctcga catctaccgc cgcctccagg ccctgaacat cctcggcgag gagcgccggc
     3781 tcgaatgggc cgacgccatt gcgcgcatcc gcaagcaacg cgagctgctc gacctcagct
     3841 atcgcgtcga caagcagcgc ctgcttcttt ccgccccggg caatcccggc aacgtcgacc
     3901 tgtacgcgag caacatgcgc gtgcagctcg ccctgctgca cgaaggcgac ctgctgcgct
     3961 tcctcgccga cctgcgcgaa tccgggaacg cctactacgg cgtgcggcgc tgcgcgctca
     4021 cccgtaccgg ccaggcggcc acaggcgcct cgatcgtccc gcggctgcgc gccgagtgcg
     4081 atatcgagct cgtcaccatg atcgatcggg cggccaagcg atgacccgcg ttgcgctctt
     4141 cctcctcctg ctttgcgttc acgtgaatcc ttgcgtggcg caagaaccgc tgggtcgcct
     4201 gtttttcacg cccgagcagc gcgacgcgct cgacgcgcgg cgcaaggcgc gcgttcccga
     4261 cagaccgacg gcaccggtcg ccgcctcgcc gaccactcgc ttcgacggct acgtgcagcg
     4321 ctccggcggc cgctcgaccg tgttcgtcaa cggcgagccg gtgaccgaag gctcgagcga
     4381 cgcgccgagc gtggatacgg ggcgcaagga cggacgcgtc ccgatcaacg tgggcgaggg
     4441 cggcggacgt cgcgcgctca agcccggcga ggtcctcgac cgcggcaccg gcgacgtgcg
     4501 cgacgtcctc ggcgaaggcg agatccgcgt ccgccgctcg ccgtgagaag gctctccggc
     4561 cgccagcgcc ggcagcacgg cgcggcgctc atcctgttcc tcacgatcct ggtgctcggc
     4621 gtcgcctggt tcgccgtcgg cgccctcggc aaggcgccgg tcgccaccgc cgaacgcgaa
     4681 gtgatcaccg ggcgtgcgct gcaggccgcc aagcaggcgc tgctcgccta cgccgcgcag
     4741 tacgccgccc gctccacgac cgccgatccg ggacagatgc cctgcccgga atcgctcacg
     4801 ctggcgaatc cgggcgaggc ttccaccagc tgcagcgcca cgctgctcgt ggtcggacgc
     4861 ctgccctgga agacgctcgg catcgacccg ctccgcgacg gcgccggcga gcccctctgg
     4921 tacatgatgc ggggcttccg cgacccgccg atcaacttcg gcaccgccgg acagctcacc
     4981 tacaacggcg ctacggtcgt cgcgatgatc atcgcccccg gtgtgccgct cagcacgacg
     5041 ggcacgccgc cagcggggtg caccgcgcag aaccagttgg tcgcgacgcg caatgccgcg
     5101 acgctcaacc cagccaattt catcgaatgc ggcgtcgcga ctggcagcat cgcgagcccc
     5161 ggcgattccg cgtggacgaa cgatcgcgcg atcgccatta ccgccgccga gtgggccgac
     5221 gcaatcgctc ccgcgatcgc cgataggctg caacgccagg ttgcgccaac gttggaggag
     5281 t
//
LOCUS       DASRIE010000056         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1621626, whole genome shotgun sequence.
ACCESSION   DASRIE010000056 DASRIE010000000
VERSION     DASRIE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621626"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..2240)
                     /locus_tag="VFB53_02640"
     CDS             complement(306..2240)
                     /locus_tag="VFB53_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HZN85660.1"
                     /translation="MKKTLGGIALCGLLLVPSAQAQTDVAGRRYTLGADSELVLRVPR
                     AWQERLGRADAKTPVTISYRPAGGAPFEVLLTPRLARGVKASLEELRPGVASMAEAAS
                     RQAAEPMLVLNELLGPEVRGYYFKATDRKAKPGEFKYLAQGLLAVGEARIAFTILTND
                     GQQAVIDDAFNLLRSARVAAGGATATAALPPGSAPRPPRPEAGKLQPLLAGGNFAELD
                     RELSAYQDAYRAGVINDEEASKAFMALVTTDADLRGAYDKWVATLPRSYVARVARGYY
                     LTRLGYQARGSQYASKTSQSQFDEMRALFKLAMADLEASLQLDAKPVLGYGTMIWITQ
                     SAGGGVPAARFLDQAIAVDRRVYTARASYLASLQPQWGGSVEQMQVALDGWKGSLEPG
                     QLERLRLMIEDSKWRVALEPAALLVNAKKYPEAIALYDRALAQQPVARAHAMRGYSQA
                     QLGEHAKAIADFDRALELDPEGACCSGTRANRARSYLTIGEVQKGINDLVIAAEAEDT
                     WASRELAMIYASGKYGFKRDYVAARRWCELSAKQGDGWAMYCLGGIYHAGLGMPKDLP
                     RAAKWFESAAKRGVPDAQTDLAYMLWTGQGIAQDRDRAVAWWRTAAKLGNKRAQQQLD
                     QHVTGWDHFTKVALPEWLGR"
BASE COUNT          318 a          817 c          746 g          387 t
ORIGIN      
        1 gccgcggccc gacaactcgc tgcgcaatgc gtccgccgac accgcgacgc cttccccgct
       61 cagcgtcgcg ccgttttccg ccagtgctgt gtatgcgaac agcggcaagg cgctcctccc
      121 cgagcggaca tgacgcgtaa cttctggttt tgattatatg tcgcgaccac tgctgaaatt
      181 tttcttgtgg ccgcgcggaa ttttttacac gtgtgcccta agcgccgcgc gaatcgcgat
      241 gacaagagtt tcgtgctcct tctgtccgat tcgcgaagga ttccagtaga cgaatcaaga
      301 gcctgctagc gccccagcca ctccgggagc gcgaccttgg tgaagtggtc ccagccggtg
      361 acgtgctggt cgagctgctg ttgcgcgcgc ttgttgccca gcttcgccgc ggtgcgccac
      421 caggcaacag cgcggtcgcg gtcctgcgcg atgccctgtc ccgtccacag catgtaggca
      481 aggtcggtct gcgcgtccgg gacccctcgc ttcgccgcgc tttcgaacca cttggcggcg
      541 cgcggcaggt ccttcggcat gccaaggccg gcatggtaga tgccgccgag gcagtacatc
      601 gcccagccgt cgccctgctt cgccgaaagc tcgcaccagc gccgcgcggc gacgtagtcg
      661 cgcttgaacc cgtacttgcc ggacgcgtag atcatcgcca gctcgcgcga ggcccaggtg
      721 tcttccgcct ccgcggcgat caccaggtcg ttgatgccct tctgcacttc gccgatggtg
      781 agatagctgc gcgcgcgatt cgcccgcgtg ccgctgcagc acgcgccctc cggatcgagc
      841 tccagggcgc gatcgaaatc ggcgatcgcc ttcgcgtgct cgccgagctg cgcctggctg
      901 tagccgcgca tcgcatgggc gcgcgcgacc ggctgctggg cgagcgcgcg gtcgtagagc
      961 gcgatcgcct ccggatactt cttggcgttc accagcagcg ccgccggctc gagcgccacc
     1021 cgccacttcg agtcctcgat catcagccgc agccgctcga gctgcccggg ttccaggctg
     1081 cccttccagc cgtcgagcgc gacctgcatc tgttccacgc tgccgcccca ttgcggttgc
     1141 aggctcgcga ggtaggaggc gcgcgcggta tacacgcggc gatcgacggc gatcgcctga
     1201 tcgagaaagc gcgccgccgg cacaccgccg ccggccgact gcgtgatcca gatcatcgtg
     1261 ccgtagccga ggaccggctt ggcgtccagc tgcagcgagg cctcgagatc ggccatcgcc
     1321 agcttgaaca gcgcgcgcat ctcgtcgaac tggctctggc tcgtcttgct ggcgtactgc
     1381 gagccgcgcg cctggtagcc caggcgtgtg aggtagtagc cgcgcgcgac gcgcgccacg
     1441 taagagcgcg gcagggtcgc cacccatttg tcataggcgc cgcgcagatc cgcgtccgtg
     1501 gtgaccagcg ccatgaacgc cttcgacgct tcctcgtcgt tgatcacgcc cgcgcggtag
     1561 gcgtcctggt atgcggacag ctcgcgatcg agctcggcga agttgccgcc cgccagcagc
     1621 ggctgcagct tcccggcttc cggccgcggc ggccgtggcg ccgagcccgg cgggagggcc
     1681 gccgtggccg tggcgccgcc ggccgctacg cgcgcactgc gcaagaggtt gaacgcatcg
     1741 tcgatcaccg cctgctggcc gtcgttcgtc aggatggtga acgcgatgcg cgcctcgccc
     1801 acggcgagca ggccctgcgc gagatatttg aactccccgg gcttggcctt gcggtcggtg
     1861 gccttgaagt agtagccgcg cacctcggga ccgagcaatt cgttgagcac cagcatcggc
     1921 tcggccgcct gccggctcgc cgcctccgcc atcgaggcca cgcccgggcg cagctcctcg
     1981 agcgaggcct tgacgccgcg cgcgagcctc ggcgtgagca gcacttcgaa cggcgcgccg
     2041 cccgcggggc gataggagat cgtgacgggg gttttcgcat cggcccggcc gaggcgctcc
     2101 tgccacgccc gtggcactcg tagcacgagc tcgctgtcgg cgccgagcgt gtagcgccgt
     2161 cccgcgacat cggtctgcgc ctgcgccgac ggcacgagca gcaatccgca cagcgcgatg
     2221 ccgccgagtg tctttttcat ggttccccct cgcgcgacta taccgcgc
//
LOCUS       DASRIE010000057         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1622011, whole genome shotgun sequence.
ACCESSION   DASRIE010000057 DASRIE010000000
VERSION     DASRIE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622011"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1297
                     /locus_tag="VFB53_02645"
     CDS             242..1297
                     /locus_tag="VFB53_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85661.1"
                     /translation="MLEQVQETLRGTPRRWLVTGAAGFIGSHLVERLLELDQNVVGLD
                     NFSTGKRANLTQVKKALGAARWRRFRLIEGDTRSRTTCREACSGAEVVLHQAALGSVP
                     LSLENPLATHDSNVTGFANILQAAREAGAQRLVYASSSAVYGDHPGVPSVEEATGQPL
                     SPYAATKQVDEAYAAVYARCFGLASVGLRYFNVFGARQDPQGAYLAVIPAWIAAMIRG
                     EPVYINGDGKTTRDFCHVDNVVRANLLAACTARAGALNQVYNIAAGEATSLNLLFELV
                     RAQLAPRFPRLAELRPQYREFRPGDIPFSVGSYAKAARLLDYQPQVRVDEGLARTVDW
                     YLEQLAPAGRRMAAHAA"
     gene            1320..2897
                     /locus_tag="VFB53_02650"
     CDS             1320..2897
                     /locus_tag="VFB53_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN85662.1"
                     /translation="MIRTLQQLLAALFAATLYAPQAAAQLCLPFLPCPDSTPPTVSIT
                     APANGATVSGVITLRANASDNQGVAGVQFRLDGNNAGAEDTGAPYEITWDTRSAPNGS
                     HVIGAVARDAAGNSASAAPVTVTVANGPPPPPPPPSATQRFEENHASVSFGAGWSASQ
                     GGWFAWSGGAALESALPGARATFNFNGTSATWIGYRSGRSGIARVFLDGVQLADVDLF
                     ARTDEPRAPILTVKDLAPGAHSLVIEATGLRNAEAIQSLVQVDAFDLPAPAVSHLQET
                     DPGMTYGGAWSQESSGIAWSAGAAKVSESTGARATLAFRGTEIAWRGYRGPDAGIARV
                     YLDGAFAAEVDLYAPSHFVQDAVYAMHGLQDGMHSIAVEVSGLRNPSASAGRVVVDAF
                     DVTAPGLRTQETDAAVSYTGTWTQGNRNRTWSEGTAAVSNTPGSRAIFSFSGTEVRWV
                     GFRAERTGIARVYLDGVFVAEVDTYAAGAEGYQATAYTIAGLAPGAHTLEIEATGRSH
                     PAATNNYVAVDAFDVVP"
     gene            2903..>3132
                     /locus_tag="VFB53_02655"
     CDS             2903..>3132
                     /locus_tag="VFB53_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85663.1"
                     /translation="MYASFAYRRSPVRLQESLIAARAALRARLREGRSFRALSAQVDD
                     SQWWSAAELRAYRERSLDNLLAFAALEVPYYR"
BASE COUNT          458 a         1171 c         1097 g          406 t
ORIGIN      
        1 cggtcgatgg gggatcgcgc gcgcggcgtg gtggagacgc tgtttacgga gcgcgtgatg
       61 gtggaccggt acgagaagct gctgacggag ctgaagtagc cgcccaacta caacgcgtcg
      121 gacccgcgcc acgcgcacca ggcagctccg gcagtgccag tccacccggc tcattgcacg
      181 ccgcaaatcg ctgaacgttg tccgtcccac aatgcctccc tcgaaatcgg aggccgaagg
      241 gatgttggaa caggtgcagg aaacgctgcg cggcaccccg cgccgctggc tggtgaccgg
      301 cgcggccggc ttcatcggct cgcacctggt cgagcgcctc ctcgagctcg accagaacgt
      361 ggtcggcctg gacaacttct ccaccggcaa gcgcgccaac ctcacgcagg tgaaaaaggc
      421 gctcggcgcg gcgcgctggc gccgcttccg cctgatcgag ggcgacaccc gctcgcgcac
      481 cacctgccgc gaggcctgca gcggcgccga ggtggtgctg caccaggcgg cgctgggcag
      541 cgtgccgctg tcgctggaga acccgctcgc cacgcacgac agcaacgtca ccggcttcgc
      601 caacatcctg caggcggcgc gcgaggcggg cgcgcagcgc ctcgtctacg ccagctcgag
      661 cgcggtgtac ggcgaccacc cgggggtgcc gagcgtggag gaggcgaccg ggcagccgct
      721 gtcgccctat gcggcgacca agcaggtgga cgaggcctac gccgcggtgt acgcgcgctg
      781 cttcggcctg gcgagcgtcg gcctgcgcta cttcaacgtg ttcggcgcgc gccaggaccc
      841 gcagggcgcc tacctcgccg tgatcccggc ctggatcgcc gccatgatcc gcggcgagcc
      901 ggtgtacatc aacggcgacg gcaagaccac gcgcgacttc tgccacgtgg acaacgtggt
      961 gcgcgccaac ctgctcgccg cctgcaccgc gcgcgccggc gcgctcaacc aggtctacaa
     1021 catcgccgcg ggcgaggcga cctcgctcaa cctgctgttc gaactcgtgc gcgcgcagct
     1081 cgcgccgcgc tttccgcgcc tcgccgagct gcgcccgcag taccgcgagt tccgccccgg
     1141 cgacattccg ttctcggtgg gcagctatgc caaggcggcg cgcctgctcg actaccagcc
     1201 gcaggtgcgc gtcgacgagg gcctggcgcg caccgtcgac tggtacctgg agcagctcgc
     1261 gcccgcggga cgccgcatgg ccgcgcacgc cgcatgaatt caaatcggaa ggaaaaccca
     1321 tgatccgcac cctgcaacag ctgcttgcgg cgctcttcgc cgccacgctc tacgcgccgc
     1381 aagccgcggc gcaactgtgc ctgccgttcc tgccctgccc ggacagcacg ccgcccacgg
     1441 tgagcatcac cgcgccggcg aacggcgcca cggtttcggg cgtgatcacg ttgcgcgcga
     1501 acgcttccga caaccagggc gtggccgggg tgcagttccg cctggacggc aacaatgcgg
     1561 gtgccgagga cacaggcgcg ccctacgaga tcacctggga cacccgcagc gcgcccaacg
     1621 gctcgcacgt gatcggcgct gtggcgcgcg acgccgccgg caacagcgcg agcgccgcgc
     1681 ccgtcaccgt caccgtggcc aacggcccgc cgcccccgcc gccgccgccc tcggcgacgc
     1741 agcgcttcga ggaaaaccac gcctcggtga gcttcggcgc gggctggagc gcctcgcagg
     1801 gcggctggtt cgcctggagc ggcggcgcgg cgctggagag cgccctgccg ggcgcgcgcg
     1861 ccacgttcaa cttcaacggc acttcggcaa cctggatcgg ctaccgcagc gggcgcagcg
     1921 gcatcgcgcg cgtgttcctc gacggcgtgc agctcgccga cgtggacctc ttcgcgcgca
     1981 ccgacgagcc gcgcgcgccg atcctcacgg tgaaggacct cgcccccggc gcgcactcgc
     2041 tggtgatcga ggccacgggc ctgcgcaacg ccgaagccat acagagcctg gtgcaggtgg
     2101 acgccttcga cctgccggcg cccgccgtgt cgcacctgca ggagaccgac cccgggatga
     2161 cctacggcgg cgcctggtcg caggagtcct cgggaatcgc ctggagcgcg ggggccgcga
     2221 aggtcagcga atccacgggc gcgcgggcca cgctcgcctt ccgcggcacc gagatcgcct
     2281 ggcgcggcta ccgcgggccg gacgccggca tcgcgcgcgt gtacctcgac ggcgcgttcg
     2341 ccgccgaggt cgatctgtac gcgccctcgc atttcgtgca ggacgcggtc tatgcgatgc
     2401 acggcttgca ggacggcatg cacagcatcg ccgtcgaggt gagcggcctg cgcaatccgt
     2461 ccgccagcgc cggtcgcgtg gtggtggacg ccttcgacgt gaccgcgccg gggctgcgca
     2521 cgcaggagac cgacgccgcg gtgagctaca ccggcacctg gacgcaaggc aaccgcaacc
     2581 gcacctggag cgagggcacg gcggcggtgt ccaacacccc cgggtcgcgc gccattttca
     2641 gcttcagcgg caccgaggtg cgctgggtcg gcttccgcgc ggagcgcacc ggcatcgcca
     2701 gggtgtacct ggacggcgtg ttcgtcgccg aggtggacac ctatgccgcc ggggcggagg
     2761 gctaccaggc caccgcctac acgatcgccg ggctggcgcc gggcgcgcac accctggaga
     2821 tcgaggcgac cggccgcagc cacccggccg ccaccaacaa ctacgtggcg gtggacgcct
     2881 tcgacgtggt gccctaggcc cgatgtacgc ctctttcgcc taccgccgca gcccggtgcg
     2941 cctgcaggag tcgctgatcg cggcgcgcgc ggcgctgcgc gcgcgcctgc gcgaggggcg
     3001 ctcgttccgc gccctgtcgg cgcaggtcga cgacagccag tggtggtcgg ccgcggagct
     3061 gcgcgcctac cgggagcgca gcctggacaa cctgctcgcc ttcgccgcgc tcgaggtgcc
     3121 gtactaccgc ga
//
LOCUS       DASRIE010000058         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2672999, whole genome shotgun sequence.
ACCESSION   DASRIE010000058 DASRIE010000000
VERSION     DASRIE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672999"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFB53_02660"
     CDS             complement(<1..756)
                     /locus_tag="VFB53_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZN85664.1"
                     /translation="MTGVLEIRGAGAPRLPLVLDSPHSGSRFPQDFGAVVSELELREA
                     EDCYVDELWGAAPALGAPLLAAHFPRTYIDPNRHAGDVDPELIEGPWPGALVPSGKAR
                     IGKSLVWRTLEDGRPIYGRRLQPEEVRRRIERYHVPYHAALRGLLDAAHARFGAVWHI
                     NCHSMRAVAGRQSDDGEGRPRADIVLGDRDGTTCEAAFTEFARATLAGMGYQVKVNDP
                     YKGVELVRAYSDPRARRHSLQIEINKRLYMDEAT"
     gene            complement(753..1400)
                     /locus_tag="VFB53_02665"
     CDS             complement(753..1400)
                     /locus_tag="VFB53_02665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85665.1"
                     /translation="MDQLAEYYAKRASEYERIYARPERQHDLAALKERVRKMLAGRRV
                     LELACGTGYWTEVFAPVAREVLAVDVNREVLDIARAKSYPAGRVEFVQGDCFAPPDFG
                     RRHDALFAGFWWSHVPLQRLDAFLASAVSSVAPRALVAFIDNRYVEGSSTPVARRDAD
                     GNGYQLRALDDGSRHEVLKNFPAESELIKRTVKHGWGANVELFEHYWLLSYWANA"
     gene            complement(1404..2363)
                     /locus_tag="VFB53_02670"
     CDS             complement(1404..2363)
                     /locus_tag="VFB53_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN85666.1"
                     /translation="MLALREVSKVFEKRLDAVERLARRLGADVQEQTVHAVDRVSFEV
                     REGEVVGLVGESGCGKSTLGRLACGLHEPSAGQVLYRGAPLDGTRRRRIQMIFQDPFA
                     SLNPRLRVGEIVGEAPRVHGLVKAGDTAAYVAGLLQRVGLDGTLARRYPHQFSGGQRA
                     RIGIARALAVQPEFLVCDEAVAALDVSIQAQVLNLFLDLRRDLGLTYLFISHDLGVVR
                     HLAERVVVMYLGRVVEIAPAAELFAAPNHPYTQALLAEVPTLERRRRSFMPIKGEIPS
                     PLAPPPGCHFHPRCPHAMARCKEENPVLREIAPGRLSACHLNG"
     gene            complement(2357..3298)
                     /locus_tag="VFB53_02675"
     CDS             complement(2357..3298)
                     /locus_tag="VFB53_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85667.1"
                     /translation="MLEVEGLVTEFATREGVLRAVNGVSFALQPGEVVGLVGESGSGK
                     SVTGYSILGLVDPPGRVAAGSVRFRGEELVGMPEARLRALRGRHIAMVFQDPMMTLNP
                     VLSVETQMVETVLAHEPGAGREAARRRALESLDRVGIAAPERRLAQYPHQFSGGMRQR
                     VAIAIALLHRPGLVIADEPTTALDVTVQGQILFEMQKLVRESGTALLWITHDLAVVAG
                     LAERILVMYAGRIVEEGSVEQVLESPRHPYTRGLLDSVPAAARPGERLRQIPGMTPSL
                     ARLGAGCAFRERCSRADAACLAMPELAGGVRCHHPLC"
     gene            complement(3291..4154)
                     /locus_tag="VFB53_02680"
     CDS             complement(3291..4154)
                     /locus_tag="VFB53_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85668.1"
                     /translation="MSEQTPLRRFVAEFAASRVALAGLALLSLIVLIALAAPWLAPQD
                     PYDLAKLDLLDSRQPPGGKYLLGTDGQGRDMLSAIFYGLRVSLYVGVASGVIALTLGA
                     LVGVFAAYVGGRFESLLMRVVDIQLGFPAILVALILIAVLGKGVDKIIFALVCVQWAY
                     YARTARSAALVERGKEYIEAARGLGLSHARIVFRHLLPNALPPLIVVGTVQVAHAIAL
                     EATLSFLGLGLPSTEPSLGLLIANGYEYLLSGRYWISMYPGIALLLAILSINLVGDQL
                     RDVLNPRLARA"
     gene            complement(4151..5125)
                     /locus_tag="VFB53_02685"
     CDS             complement(4151..5125)
                     /locus_tag="VFB53_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85669.1"
                     /translation="MTVYILRRLSQTLLVLAVTSALVFGGLYLVGDPVEILVNPNADQ
                     IEKDRTAKALGLDKPVLEQYWVFVRGAVTGNLGTSFVFGRPAMEVILERMPATLEIAF
                     LAMLMSVGLGIPLGLYAGLRPHGFGARAIMAGSILGFSLPTFWVGLLLIMVFAVQLGW
                     LPSTGRGPTVEFLGLQLSILTWEGLRYALLPALNLALFKLSLIIRLTRAQVREQWLLD
                     YIRFARAKGLPRSRVIGVHLLKNILIPLVTVIGLELGSVIAFAVVTETVFAWPGMGKL
                     VIDSIFQLDRPVVVAYLLVTVFMFILINLAVDLLYSALDPRVRLQEAK"
     gene            complement(5133..>5638)
                     /locus_tag="VFB53_02690"
     CDS             complement(5133..>5638)
                     /locus_tag="VFB53_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85670.1"
                     /translation="NGFGLTLHTPNNRYINDEKIAQAVAQFLTRGGIPTKVEAMPSAV
                     FFSRGSKLEFSFLLAGWGAETGENSSPLRSLLGTFDSAKGIGGANRGRFSDAGVDALI
                     ETALTTIDDTKRSLILAAATEKAVGELQGLIPVHYEVSTWATRKGITYKARADQYTLA
                     HEVRLGN"
BASE COUNT          883 a         2027 c         1979 g          749 t
ORIGIN      
        1 cgtggcctcg tccatgtaca ggcgcttgtt gatctcgatc tgcaggctgt ggcgccgcgc
       61 gcgcgggtcc gaataggcgc gcaccagctc gacgcccttg taggggtcgt tgaccttcac
      121 ctggtagccc atgccggcga gcgtggcgcg cgcgaactcg gtgaacgccg cctcgcaggt
      181 ggtgccgtcg cggtcgccga gcacgatgtc ggcgcgcggc cgaccttccc cgtcgtcgct
      241 ctggcggccc gccaccgcgc gcatggagtg gcagttgatg tgccacaccg cgccgaagcg
      301 cgcgtgcgcg gcgtcgagca gcccgcgcag cgccgcatga taggggacgt ggtagcgctc
      361 gatgcgccgg cgcacttcct cgggctgcag ccggcggccg tagatcgggc ggccgtcttc
      421 cagcgtgcgc cagaccaggc tcttgccgat cctcgccttg ccgctcggca ccagcgctcc
      481 cggccagggg ccctcgatca gctccgggtc cacgtcgccg gcgtggcggt tgggatcgat
      541 gtaggtgcgc ggaaaatgcg ccgccagcag cggcgcgccg agcgccggcg cggcgcccca
      601 gagctcgtcg acgtagcagt cctcggcctc gcgcagctcc agctcgctca ccaccgcacc
      661 gaagtcctgc gggaagcggc tccccgagtg cggcgaatcc agcaccagcg gcagccgcgg
      721 cgcgcccgcg ccgcggatct cgagcacccc cgtcatgcgt tggcccagta gctgagcagc
      781 cagtagtgct cgaagagctc cacgttggcg ccccagccgt gcttcaccgt gcgcttgatc
      841 agctcgctct cggcgggaaa gttcttcagc acctcgtggc ggctgccgtc gtcgagcgcg
      901 cgcagctggt agccgttgcc gtcggcgtcg cggcgcgcga cgggggtgct cgagccctcg
      961 acgtagcggt tgtcgatgaa ggcgaccagc gcgcgcggcg ccacgctcga caccgcggag
     1021 gcgagaaagg cgtccaggcg ctgcagcggc acgtgcgacc accagaaccc ggcgaacagc
     1081 gcgtcgtgcc ggcggccgaa atccggaggc gcaaagcagt cgccctgcac gaactcgacg
     1141 cgccccgccg gataggactt ggcgcgcgcg atgtcgagga cctcccggtt cacgtccacg
     1201 gcgagcactt ccctggccac cggcgcgaat acctccgtcc agtagccggt gccgcaggcg
     1261 agctcgagca cgcgccgccc tgcgagcatc ttgcgcaccc gctccttcag cgcggcgagg
     1321 tcgtgctggc gctccggcct ggcatagatg cgttcgtatt ccgatgcacg cttggcgtaa
     1381 tactcggcga gctggtccat gggctatccg ttcaggtggc aggcggagag gcgtccgggc
     1441 gcgatttcgc gcaacaccgg attctcctcc ttgcagcgcg ccattgcatg cgggcagcgc
     1501 gggtggaagt ggcaccccgg gggcggcgcc agcggcgagg ggatctcccc cttgatcggc
     1561 atgaagctgc ggcgccggcg ctccagcgtc ggcacctcgg cgagcagcgc ctgcgtgtag
     1621 ggatggttcg gcgcggcgaa cagctcggcc gccggcgcga tctcgaccac gcgccccagg
     1681 tacatcacca ccacccgctc cgcgaggtgg cgcaccaccc ccaggtcgtg gctgatgaac
     1741 agatacgtca accccaggtc gcggcgcagg tcgaggaaca ggttcaatac ctgcgcctgg
     1801 atcgacacgt ccagggcggc cacggcctcg tcgcacacca ggaactccgg ctgcaccgcg
     1861 agcgcgcgcg cgatgccgat gcgcgcgcgc tgcccgccgg agaactggtg cgggtagcgg
     1921 cgcgccagcg tgccgtcgag gcccacgcgc tgcagcaggc ccgcgacgta ggccgcggtg
     1981 tccccggcct tcaccagccc gtgcacgcgc ggcgcctcgc ccacgatctc gccgacgcgc
     2041 aggcgcgggt tgagcgaagc gaaggggtcc tggaagatca tctggatgcg ccggcgccgc
     2101 gtgccgtcca gcggcgcccc gcggtacagc acctgcccgg cgctcggctc gtgcaggccg
     2161 caggcgaggc gccccagcgt ggacttgccg cagcccgatt cgcccaccag ccccaccacc
     2221 tccccctcgc gcacctcgaa gctcacccgg tcgaccgcgt gcaccgtttg ctcctgcacg
     2281 tccgcgccga gcctgcgcgc cagccgctcc accgcgtcca ggcgcttctc gaagaccttg
     2341 ctgacctcgc gcagcgctag cacagcgggt ggtggcagcg tacgccgccg gcgagttcgg
     2401 gcatggcgag gcaggcggca tcggcgcggc tgcagcgctc gcggaaggcg cagccggcgc
     2461 ccaggcgcgc cagcgacggc gtcatgccgg gaatctggcg caggcgctcg ccgggccgcg
     2521 cggcggccgg caccgagtcg agcaggccgc gcgtgtacgg gtggcgcggc gactccagca
     2581 cctgctcgac gctgccctcc tccacgatgc gcccggcgta catgacgagg atgcgctcgg
     2641 cgagtcccgc caccaccgcg aggtcgtgcg tgatccacag cagcgcggtg cccgattcgc
     2701 gcaccagctt ctgcatctcg aacaggatct gcccctgcac ggtgacgtcg agggcggtgg
     2761 tcggctcgtc ggcgatcacc agcccggggc ggtgcagcag cgcgatggcg atcgccacgc
     2821 gctggcgcat gccgcccgag aactggtgcg gatactgcgc caggcgccgc tcgggcgcgg
     2881 cgatgcccac gcgatcgagc gactcgaggg cgcgccggcg cgccgcctcg cggccggcac
     2941 cgggctcgtg cgccagcacc gtctccacca tctgcgtctc gaccgagagc accggattga
     3001 gcgtcatcat cgggtcctgg aacaccatgg cgatgtgccg gccgcgcagc gcgcgcagcc
     3061 gcgcctcggg cattcccacc agctcctcgc cgcgaaagcg cacgctgcct gcggcgacgc
     3121 gccccggcgg gtccaccagg ccgaggatgg agtagccggt gaccgatttg cccgagccgg
     3181 actcgcccac caggcccacc acctcccccg gctgcagcgc gaagctcacg ccgttcaccg
     3241 cgcgcagcac cccctcgcgc gtggcgaact cggtcaccag gccctcgacc tcaagcacgc
     3301 gccagcctcg ggttgagcac gtcgcgcagc tggtcgccga ccaggttgat ggagaggatc
     3361 gcgagcagca gcgcgatgcc cgggtacatg ctgatccaat agcggcccga gagcaggtac
     3421 tcgtagccgt tggcgatgag caggccgagc gagggctcgg tgctcggcag ccccaggccg
     3481 aggaaggaca gcgtcgcctc gagcgcgatg gcgtgcgcca cctgcacggt gccgaccacg
     3541 atcagcggcg gcagcgcgtt gggcagcagg tggcggaaca cgatgcgcgc gtgcgacagg
     3601 cccaggccgc gcgccgcctc gatgtactcc ttgccgcgct ccaccagcgc cgccgagcgc
     3661 gcggtgcgcg cgtagtaggc ccattgcacg cacaccagcg cgaagatgat cttgtccacg
     3721 cccttgccca gcaccgcgat caggatcagc gccaccagga tggcgggaaa gccgagctgg
     3781 atgtccacca cgcgcatcag caggctctcg aagcggccgc ccacgtaggc ggcgaaaacg
     3841 ccgaccagcg cgcccagcgt cagcgcgatg accccgctcg ccacgccgac gtagagcgac
     3901 acgcgcagcc cgtagaagat ggccgagagc atgtcgcggc cctgcccgtc ggtgccgagc
     3961 aggtacttgc cgcccggcgg ctggcgcgag tccagcagat cgagcttcgc gaggtcgtag
     4021 ggatcctgcg gcgccagcca cggcgccgcg agggcgatca ggacgatcag cgagagcagc
     4081 gcgaggccgg cgagcgccac gcgcgaggcg gcgaattcgg cgacgaagcg ccgcaggggc
     4141 gtctgctcgc tcacttcgcc tcctgcaggc gcacgcgcgg gtcgagcgcc gagtacagca
     4201 ggtccaccgc caggttgatg aggatgaaca tgaacaccgt caccagcagg taggccacca
     4261 ccaccgggcg gtcgagctgg aagatcgaat cgatcaccag cttgcccatt cccggccagg
     4321 cgaacaccgt ctcggtgacc acggcgaagg cgatcaccga gccgagctcc aggccgatca
     4381 ccgtgaccag cgggatgagg atgttcttca gcaggtgcac gccgatcacg cgcgagcgcg
     4441 gcaatccctt ggcgcgcgcg aagcggatgt agtcgagcag ccactgctcg cgcacctgcg
     4501 cgcgcgtcag gcggatgatc agcgagagct tgaacagcgc caggttgagc gccggcagga
     4561 gcgcgtagcg caggccctcc caggtcagga tcgacagctg caggccgagg aattcgacgg
     4621 tgggcccgcg cccggtcgag ggcagccagc ccagctgcac cgcgaacacc atgatcagca
     4681 gcagcccgac ccagaaggtg ggcagcgaaa agccgaggat cgagccggcc atgatcgccc
     4741 tggcgccgaa gccgtgcggg cgcaggcccg cgtacagccc gagcgggatg cccaggccca
     4801 ccgacatcag catggcgagg aaggcgatct ccagcgtcgc cggcatgcgc tccaggatca
     4861 cctccatggc agggcggccg aaaacgaagg acgtgccgag gttcccggtc acggcgccgc
     4921 gcacgaagac ccagtactgc tcgagcaccg gcttgtccag gccaagcgcc ttggcggtac
     4981 ggtccttctc gatctggtcc gcgttcgggt tgacgaggat ctcgaccggg tcgccgacca
     5041 agtacagacc gccgaagacg agcgcggagg taacggccag gacgagcagc gtctgagaga
     5101 gccgccgcag gatgtagacc gtcaccgcca gcctagttgc cgagccggac ctcgtgcgcg
     5161 agggtgtact ggtcggccct cgccttgtag gtgatgcctt tgcgcgtggc ccaggtgctc
     5221 acctcgtagt ggaccgggat caggccttgc agctcgccta ctgccttctc cgtcgccgcc
     5281 gccaggatga gcgagcgctt ggtgtcgtcg atggtggtga gcgcggtctc gatcagggcg
     5341 tccacgccgg cgtcggaaaa gcggccgcgg ttcgcgccgc cgatgccctt cgcggagtcg
     5401 aacgtgccga gcagcgagcg cagcggcgag ctgttctcgc cggtctccgc gccccatccg
     5461 gcgagcagga aggagaactc gagcttggag ccgcgcgaga agaacactgc cgagggcatc
     5521 gcctcgacct tggtcgggat gccgccgcgc gtgaggaact gcgcgaccgc ctgggcgatc
     5581 ttctcgtcgt tgatgtagcg gttgttcggc gtatgcagcg tgaggccgaa gccgttcg
//
LOCUS       DASRIE010000059         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_321786, whole genome shotgun sequence.
ACCESSION   DASRIE010000059 DASRIE010000000
VERSION     DASRIE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321786"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFB53_02695"
     CDS             complement(<1..237)
                     /locus_tag="VFB53_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85671.1"
                     /translation="MAQGAFKSLARVVANERGLFIGALILGGVALEVGVPLDFVLFGL
                     TLAGVALFHRHVLWVALIGAAVIALYKIGFTGFKT"
     gene            complement(289..1410)
                     /locus_tag="VFB53_02700"
     CDS             complement(289..1410)
                     /locus_tag="VFB53_02700"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZN85672.1"
                     /translation="MDDLHFTPSPELSIGLELELQLVQSHDLDLARDAAELLARLAKQ
                     PLPGAVKPEITESMIELNTSVHARHATLAAELAMMRDVVVREAGMLNLRVCGGGAHPF
                     HSWSERRIFPTERFKHVLERYGYLAKQFTVFGQHIHVGCPHGDDAVTLVHLMTRYVPH
                     FIALSAASPFYQGEDTQFQSSRLTAVNAFPLAGHMPFVADWAEFLQYFGKMRGFGVVE
                     SMKDFYWDIRPKPEYGTIEIRVCDTPLSVARAAQLAAYAQALAKWLLEERPRPAARAV
                     YLVNSFNRFEAARYGLRGQFIDPYEEKKKPLAEDILDAIAGVMPQAVALECADALVAL
                     AADVRALYSDADWLRGQRGPGGALSDVVRKMAERWAAAP"
     gene            complement(1423..2634)
                     /locus_tag="VFB53_02705"
     CDS             complement(1423..2634)
                     /locus_tag="VFB53_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN85673.1"
                     /translation="MELGFLPDLPAALDAPALFGALLLAGLLVGEGVRRWLGLPRITG
                     YVLAGVLLGPRASGLLGPGALDEARVLIDLSLGLIVFELGHRLDFQWLRRNRWLYAAA
                     IAESVVCFAAIHVALLAFGFAPLLSASAAAIGTATSPAVVLLVAHELRAAGQVTERML
                     LFTAVNCVFAYVTLTLLLPFLHLEHEMDWPEALLHPLYILGGSALAGFVACLLMLWVA
                     RWVGKREDRQFILLVALVVLAVGLARALHLSVVVTLITAGLLARNLDRHHVLLPLRFG
                     YGGQLFFVVLFVLTGASLQLGALPAAFAAAGSFILARFLGKGLAVLSFAPLSGMRPGA
                     AGWLTVSLLPMSGLAVVMVLDTTAMYPQFGAELAAVVLSAAAVLELFGPIATQFALQR
                     VGEADPAATEA"
     gene            complement(2635..>3041)
                     /locus_tag="VFB53_02710"
     CDS             complement(2635..>3041)
                     /locus_tag="VFB53_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZN85674.1"
                     /translation="KYLRDKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDE
                     INADFPDTDVAMVIGANDIVNPAAQDDPTSPIAGMPVLEVWKAKTSIVMKRSMASGYA
                     GVDNPLFYKENNRMLFGDAKKMLDEVLAALKG"
BASE COUNT          495 a         1102 c         1058 g          386 t
ORIGIN      
        1 ggtcttgaag ccggtgaagc cgatcttgta cagggcgatg accgcggcgc cgatgagcgc
       61 cacccacagc acgtggcggt ggaacagcgc gacccccgcg agcgtcaggc cgaacagcac
      121 gaagtccagc ggcaccccga cctcgagcgc cacgccgccc aggatcagcg ccccgatgaa
      181 caggccgcgc tcgtttgcca cgacgcgcgc cagcgacttg aatgccccct gcgccatgcc
      241 ctcccccttg tcggtcgcgg cccgattata ggggcggcgc cggccccctc agggcgccgc
      301 ggcccaccgt tccgccatct tgcgcaccac gtcgctcagc gccccgccgg ggccgcgctg
      361 cccgcgcagc cagtcggcgt ccgaatacag cgcgcgcacg tcggccgcca gcgccaccag
      421 ggcgtcggcg cactccagcg ccaccgcctg cggcatcacg ccggcgatgg cgtcgaggat
      481 gtcctcggcg agcggcttct ttttctcctc gtacgggtcg atgaactggc cgcgcaggcc
      541 gtagcgcgcc gcctcgaagc ggttgaagct gttcaccagg tacaccgcgc gcgccgccgg
      601 gcgcggccgc tcctccagca gccacttggc gagcgcctgc gcgtaggcgg cgagctgcgc
      661 cgcgcgcgcc acgctgagcg gcgtgtcgca gacgcggatc tcgatggtgc cgtactccgg
      721 cttggggcgg atgtcccagt agaagtcctt catgctctcc accacgccga agccgcgcat
      781 cttgccgaag tactgcagga actcggccca gtcggcgacg aagggcatgt ggccggcgag
      841 cgggaaggcg ttgaccgcgg tgaggcgcga ggactggaac tgcgtgtcct cgccctggta
      901 gaagggcgag gccgccgaca gcgcgatgaa gtgcggcacg tagcgcgtca tcaggtgcac
      961 cagggtcacc gcgtcgtcgc cgtgcgggca gccgacgtgg atgtgctggc cgaacacggt
     1021 gaactgcttg gccaggtagc cgtagcgctc gagcacgtgc ttgaagcgct cggtggggaa
     1081 gatgcgccgc tcgctccagc tgtggaacgg gtgcgcgccg ccgccgcaca cgcgcaggtt
     1141 gagcatgccg gcctcgcgca ccaccacgtc gcgcatcatc gccagttcgg cggccagcgt
     1201 ggcatggcgc gcgtgcaccg aggtgttcag ttcgatcatg ctctcggtga tctcgggctt
     1261 caccgcgccg ggcagcggct gtttcgccag gcgcgccagc agctcggcgg cgtcgcgcgc
     1321 caggtccagg tcgtgcgatt gcaccagctg cagctccagc tccagcccga tcgacagttc
     1381 cggcgacggc gtgaagtgca ggtcgtccac ggcgccgccg cgctaggcct cggtggccgc
     1441 ggggtcggct tcgccgacgc gctgcagcgc gaactgcgtg gcgatggggc cgaacagctc
     1501 cagcaccgcc gccgccgaca gcaccaccgc cgccagctcg gcgccgaact gcggatacat
     1561 ggcggtggtg tcgagcacca tcaccacggc gaggccggac atcggcagca gcgacaccgt
     1621 gagccagccg gcggcgcccg ggcgcatgcc cgacagcggg gcgaaggaca gcaccgccag
     1681 gcccttgccc aggaagcgcg ccaggatgaa cgaccccgcc gccgcgaacg ccgcgggcag
     1741 cgcgccgagc tgcaggctcg ccccggtgag cacgaacagc acgacgaaga acagctgccc
     1801 gccgtagccg aagcgcagcg gcagcagcac atggtgccgg tcgaggttgc gcgccagcag
     1861 gcccgcggtg atcagcgtca ccaccaccga caggtgcagg gcgcgcgcca ggcccaccgc
     1921 cagcaccacc agcgcgacca gcaggatgaa ctgccggtcc tcgcgcttgc cgacccagcg
     1981 cgcgacccac agcatcagca ggcaggcgac gaagcccgcg agcgccgagc cgcccaggat
     2041 gtacagcgga tgcagcagcg cctcgggcca gtccatctcg tgctccaggt gcaggaacgg
     2101 cagcagcagc gtcagcgtga cgtaggcgaa cacgcagttc accgcggtga acagcagcat
     2161 gcgctcggtc acctggccgg cggcgcgcag ctcgtgcgcc accagcagca cgacggcggg
     2221 cgaggtggcg gtgccgatgg cggcggcgct cgccgagagc agcggcgcga agccgaaggc
     2281 gagcagcgcg acgtggatgg cggcgaagca cacgacgctc tcggcgatgg cggccgcgta
     2341 cagccagcgg ttgcgccgca gccactggaa atccaggcgg tggcccagct cgaacacgat
     2401 caggccgagc gacaggtcga tcagcacgcg cgcctcgtcc agcgcgccgg gcccgagcag
     2461 gcccgaggcc cgcggcccga gcagcacccc ggcgagcacg tagccggtga tgcgcggcag
     2521 gccgagccag cggcgcacgc cctcgcccac cagcagcccg gcgagcagca gcgcgccgaa
     2581 cagcgccggg gcgtcgagcg cggcgggcag gtccggaagg aagcccagct ccatctaccc
     2641 cttcagcgcg gccagcacct cgtcgagcat cttcttcgcg tcgccgaaca gcatgcggtt
     2701 gttctccttg tagaagagcg ggttgtccac gccggcgtag ccggaggcca tgctgcgctt
     2761 catcacgatc gaggtcttcg ccttccacac ttccagcacc ggcatgccgg cgatcgggct
     2821 ggtcggatca tcctgcgcgg ccggattcac gatgtcgttt gcgccgatca ccatcgctac
     2881 gtcggtgtcg ggaaaatcgg cgttgatctc gtccatctcc agcacgatat cgtaggggac
     2941 cttcgcctcg gcgagcagca cgttcatgtg gcccggcatg cgtccggcga ccggatggat
     3001 gccgaagcgc acgttcacgc ccttgtcgcg cagatacttc g
//
LOCUS       DASRIE010000060         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_832499, whole genome shotgun sequence.
ACCESSION   DASRIE010000060 DASRIE010000000
VERSION     DASRIE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832499"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1003
                     /locus_tag="VFB53_02715"
     CDS             125..1003
                     /locus_tag="VFB53_02715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85675.1"
                     /translation="MTDSKILGFIGTGVMGEPMCRNLAAKSGRRVIAWDPSPAPLERL
                     RAAGVAIAVSPAELAAQASILFLCLPGGAQLKALCEDQLLAAARGGLTLVDLGTSGVG
                     LTRALAQRFAARGADYADAPIARTRAAAESGTLSISVGAAPQVFGRIAPFLRCLGSDI
                     THCGGVGGGQLVKILNNMVLFQTVHALAEALALGRRAGMDPALLFDALSKGSADSFAL
                     RNHGAKALLPGAYPERAFSVEYAAKDNGYALDLGRETGVRLRGAELLQEVLAEARAGG
                     YGAAYWPVIAKVVDRA"
     gene            complement(1011..1736)
                     /locus_tag="VFB53_02720"
     CDS             complement(1011..1736)
                     /locus_tag="VFB53_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZN85676.1"
                     /translation="MRPLLFALALAHAAVPVAQAAHPLITEDTGTQGAGGWQLELFGE
                     EGKARGTGATLERQDAVVSYGIAGTADLQLGLPWMRHAGGRGPGDLSLDLKWRFLARG
                     PLSLGLKPGVTLPTGDEAKGLGAGRAAWGALLILSYDAGPYAIHAHAGYKRNRNDLGE
                     RESLAHASGAVAVQAAPNVKVVLDYARNTSADTAATTSERYLVFGAIWSVSKAFDLDL
                     GFKVGSGSAALDQALLLGATARW"
     gene            1793..2089
                     /locus_tag="VFB53_02725"
     CDS             1793..2089
                     /locus_tag="VFB53_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN85677.1"
                     /translation="MPYAILTTDKPDSGALRAKVRDEHLKYLDANAYRLLAAGAVISD
                     DGTGGHGGILLVDTDLRQEAEEFIAGDPFTKAGLFAQVKVTRWRKAYFDKKRLI"
     gene            complement(2086..2592)
                     /locus_tag="VFB53_02730"
     CDS             complement(2086..2592)
                     /locus_tag="VFB53_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HZN85678.1"
                     /translation="MEATTPQTSRRRFFKLAGITTAAAGLGAVALGALAHGRRHGGGP
                     VDPAKMEQHLDRMLRHMYVEIDATDAQKQKLDPIVKQAAKDLAPLREQVRAARQEGVK
                     LLSADTIDRGAIERFRVQQIGATDAASRRFTQALADVAEVLTPEQRRKLAAHAGRRRH
                     GRGGMAFG"
     gene            complement(2679..3791)
                     /locus_tag="VFB53_02735"
     CDS             complement(2679..3791)
                     /locus_tag="VFB53_02735"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN85679.1"
                     /translation="MTRAACILLALAAAPLAAQQLHRSEEHAFRVVELVRGLEQPWSL
                     AFLPDGRQLVTEKVGRLRLVAGGKLDPQPVAGLPQVTVHGQGGLMDVALHPGFAENGL
                     VYLSYAARGEDGVGTEVARGKLVGHRLEAVQVLFRQDPKGGRGQHFGSRLVFDRQGFL
                     YITLGDRGEMARAQKPDDHAGSVIRLHDDGRVPADNPYAGRPGWKPEKYTLGNRNQQG
                     AALHPVTGQLWTHEHGPQGGDEVNLIRAGANYGWPVITYGVNYGIGTKIGEGTAKAGM
                     EQPLHKWVPSIAPSGMAFYAGDKFPKWRGDLFVGALRAELLVRLRFDGTRFLHEERLL
                     RGELGRIRDVRAGPDGLIYLLTDESRAVLARLEPAG"
     gene            3964..4146
                     /locus_tag="VFB53_02740"
     CDS             3964..4146
                     /locus_tag="VFB53_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85680.1"
                     /translation="MEIRPGFALAAFVAALLAVGFSAAYASQPAPGTAYDQQQQAPKD
                     CKMKPEDPRCKDEPKG"
     gene            complement(4148..5353)
                     /locus_tag="VFB53_02745"
     CDS             complement(4148..5353)
                     /locus_tag="VFB53_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN85681.1"
                     /translation="MQNSAAARPLDGIRVLEMGQLLAGPFAGCVLGYFGAEVIKIEPP
                     RGDPIRGWREVRDGTSLWWASLARNKKSVTLDLQSARGRELARALAAKSDVLIENFRP
                     GTMEKWGLGPEELKRLRPELIYARVSGYGQTGPYAARPGFASVCEGFAGFRYLNGVPG
                     EAPVRPNISMGDTLAGLHAVLGILLALAARLRGKAAAGQVVDVAIYESVFNMLEGVVP
                     EYDGAGVVREPSGSTITGIVPTNTYRCADGKYVVIGGNGDSIYQRLMRAIGRPEMAED
                     ARYASNALRVQNERAIDAAISAWTGARDAAEVLAALRDAEVPNGPIYSVADMMADPHF
                     NARGMFETVSVGGKPLKIPAIPPLLGETPGRTDAPGPALGAHTDEVLETVLGLPAAAR
                     DELRAAGVV"
     gene            5430..6392
                     /locus_tag="VFB53_02750"
     CDS             5430..6392
                     /locus_tag="VFB53_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HZN85682.1"
                     /translation="MTSRRIALGAALAAAVVLATPSFAQERTLRILVGFPPGGTTDIL
                     ARLLAERLRAPLGQNVIVENKPGAVASIAAAEVKNSPADGSTLLMSVVGAMTVAPHAR
                     KSNIFDTLKDFAPVSLVTNAHLAIAVGPGSPAKDLKGFLAWAKGNPGKAFYATSGAGT
                     LPHMLGLLLAQEAGVQLTHVPYKGTSAYINELMGGQVPAAFDAMGDLSEQHKAGRVRI
                     LASAGTARSRAMPEIPTMREQGYNIEGAAWFGLFAPAATPRGTVERLSQEVARVLQGA
                     DIGAKIASLNMEPVGSTPEEFQRVVRADWAKWGAVVRASGFTID"
     gene            6423..>6789
                     /locus_tag="VFB53_02755"
     CDS             6423..>6789
                     /locus_tag="VFB53_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85683.1"
                     /translation="MKREAPPADAIVIRGARQNNLKNLTLSLPANELIVVTGVSGSGK
                     SSLVFDTLYAEGQRRYVETFSPYARQFLERMDKPQVDAIEGIPPAIAIDQTNPVRTSR
                     STVGTMTELNDHLKLLFARG"
BASE COUNT          986 a         2406 c         2398 g          999 t
ORIGIN      
        1 ccctgcccgg tcagcagccg caggtcctcc agccgccgca acgggtggcc gatccactgg
       61 ctggaggaat gtgcgacgtc gttcacgcag cgggcgattc tatactgcgg gcaaggagag
      121 tttcatgacc gattcgaaga tcctcggttt catcggcacc ggcgtgatgg gcgagcccat
      181 gtgccgcaac ctcgcggcga agagcgggcg ccgcgtcatt gcctgggacc cgtcccctgc
      241 gccgctcgag cgcctgcgcg cggcgggcgt cgcgatagcg gtctcgcccg ccgaacttgc
      301 cgcgcaggcg tcgatcctgt tcctctgcct gcccggcggc gcgcagctga aggcactgtg
      361 cgaagaccag ttgctcgccg cggcgcgcgg cggcctgacc ctggtggacc tcggcacttc
      421 cggcgtgggg ctaacgcgcg cgctggcgca gcgattcgct gcgcgcggcg cggactacgc
      481 cgatgcgccc atcgcgcgca cgcgtgcagc cgccgaatcc ggcacgctca gcatcagcgt
      541 cggcgctgcg ccgcaggtgt tcgggcgcat cgcgccgttc ctgcgctgcc tgggctccga
      601 catcacccat tgcggcggcg tgggcggcgg ccagctggtg aagatcctca acaacatggt
      661 gttgttccag acggtccacg cgctcgccga ggcgctcgcg ctcgggcggc gagccggcat
      721 ggatcccgcg ctcctgttcg acgcgctgtc caaggggtcg gccgacagct tcgcgctgcg
      781 caaccatgga gcgaaggcgc tgctgccggg cgcatacccg gagcgcgctt tctcggtcga
      841 atacgcggcg aaggacaacg gctacgcgct cgacctgggc cgcgagacgg gcgtgcggct
      901 gcgcggggcg gaactgctgc aggaggtgct ggcggaagcg cgcgccggag gctacggcgc
      961 ggcctactgg ccggtgatcg cgaaagtcgt ggaccgcgcc tagcgccgcg ctaccagcgc
     1021 gcggtggcgc cgagcagcag cgcctggtcg agggcggcgc tgccgctgcc gaccttgaaa
     1081 ccgaggtcga ggtcgaaggc cttgctcacc gaccagatgg cgccgaatac gaggtagcgc
     1141 tcggatgtcg tggcggccgt gtcggcgctg gtgttgcgcg cgtaatcgag caccactttc
     1201 acgttgggcg cagcctgcac ggcgacggcg cccgaggcgt gcgcgagcga ttcgcgctcg
     1261 cccaggtcgt tgcggttgcg cttgtagccc gcgtgggcgt ggatggcgta gggccccgcg
     1321 tcgtagctca ggatgagcag cgcgccccag gcggcgcggc cggcgccgag gcccttcgct
     1381 tcgtcgccgg tgggcagcgt gacgccgggc ttgaggccga ggctgagcgg cccgcgcgcc
     1441 aggaaccgcc atttcaggtc cagggacagg tcgcccgggc cgcgcccgcc cgcgtgccgc
     1501 atccagggca ggccgagctg caggtcggcg gtcccggcga tgccgtagct cacgaccgcg
     1561 tcctggcgct cgagcgtcgc gcccgtgccg cgcgccttgc cttcttcgcc gaacagctcg
     1621 agctgccagc cgcccgcacc ctgcgtgccg gtgtcttcgg taatgagcgg atgcgccgcc
     1681 tgcgcaacgg ggacggccgc gtgggcgagg gctagcgcga aaagaagcgg ccgcatgatt
     1741 ttgtgcggcg cgagtttaaa ctatgtgatc ccaaccgaaa cggagaattc gcttgcctta
     1801 cgccatcctg acgaccgaca agccggactc cggcgcgctg cgcgccaagg tgcgcgacga
     1861 gcacctgaag tacctcgacg ccaacgccta ccggctgctc gccgcgggcg cggtgatcag
     1921 cgacgacggc accggcgggc acggcggcat cctgctcgtg gataccgacc tgcgccagga
     1981 ggccgaggag ttcatcgccg gcgacccctt caccaaggcg ggtctgttcg cccaggtgaa
     2041 ggtgacgcgc tggcgcaagg cctacttcga caagaagcgg ctgatctagc cgaacgccat
     2101 cccgccgcgg ccgtgccggc ggcgcccggc gtgcgccgcg agcttgcggc gctgctcggg
     2161 cgtgagcacc tcggcgacgt cggcgagcgc ctgcgtgaag cgcctggacg ccgcgtcggt
     2221 ggcgccgatc tgctgtacgc ggaagcgctc gatggcaccg cggtcgatgg tgtcggccga
     2281 cagcagcttg acgccttcct gccgcgccgc gcgcacctgc tcgcgcagcg gcgcgaggtc
     2341 cttcgccgcc tgcttcacga tcgggtcgag cttctgcttc tgcgcgtcgg tggcgtcgat
     2401 ctcgacgtac atgtgccgca gcatgcggtc gaggtgctgc tccatcttcg ccgggtcgac
     2461 cggcccgccg ccgtggcgcc ggccgtgcgc cagcgcgccg agcgcgaccg cgccgaggcc
     2521 ggccgcggcc gtggtgatgc cggcgagctt gaagaagcgg cgccgggacg tttggggagt
     2581 cgttgcttcc atgttcgatc ctttcagagt gggaaacgcg gcgccagtgt gcggcgcagg
     2641 cgtaaggcgc tgatgaccac tcgtaagcag atgtaacctc agcccgccgg ctcgaggcgc
     2701 gcgagcacgg cgcgcgattc gtcggtcagc aggtagatca ggccgtcggg cccggcgcgc
     2761 acgtcgcgga tgcggcccag ctcgccgcgc agcaggcgct cctcgtgcag gaaccgcgtg
     2821 ccgtcgaagc gcaggcgcac cagcagctcg gcgcgcagcg cgccgacgaa cagatcgccg
     2881 cgccacttcg ggaatttgtc cccggcgtag aacgccatgc ccgaaggcgc gatcgagggc
     2941 acccatttgt gcagcggctg ttccatgccg gccttcgcgg tgccctcgcc gatcttcgtg
     3001 ccgatgccgt agttgacccc gtaggtgatc acgggccagc cgtagttcgc cccggcgcgg
     3061 atcaggttca cctcgtcgcc gccttgcggt ccgtgctcgt gggtccagag ctgcccggtg
     3121 accgggtgca gcgccgcgcc ctgctggttg cggttgccca gcgtgtactt ctctggcttc
     3181 cagcccggcc ggccggcgta ggggttgtcc gcgggcacgc ggccgtcgtc gtgcaggcgg
     3241 atcaccgaac cggcgtggtc gtccggcttc tgcgcgcgcg ccatctcgcc gcggtcgccg
     3301 agggtgatat agaggaaacc ctgccggtcg aacaccaggc gcgagccgaa gtgctggccg
     3361 cgtccgccct tcgggtcctg ccggaacagg acctgcacgg cttccaggcg atggccgacc
     3421 agcttgccgc gcgccacttc ggtgcccacg ccgtcctcgc cgcgggccgc ataggacagg
     3481 tagacgaggc cgttttccgc gaacccgggg tgcagcgcga cgtccatcag gccgccctgc
     3541 ccgtgcaccg tgacctgcgg caggccggcg accggctgcg ggtcgagctt gccgccggcg
     3601 accaggcgca gccgcccgac cttctcggtg accagctgtc gtccgtcggg aaggaaggcg
     3661 agcgaccagg gttgctccag gccgcgcacc agctcgacga cgcgaaacgc gtgctcctcg
     3721 gagcggtgca gctgctgcgc cgccagcggc gcagcggcca gggcaaggag gatgcaggct
     3781 gctcgggtca tggcgaaacg ctatcacggc ccggctgttg ggcgcgcccg acaggcgggc
     3841 ttgtgcgatt gcctccgaag tcggcgctaa ccgcctgatc ccccttgcaa tcgggctgtt
     3901 ggcacgaact tcgcaggaca tcgggtgttg gaccgttccg tcaaccgatg aaaggagtcg
     3961 atcatggaaa tcagacccgg attcgcactg gcggcgttcg tcgcagcctt gctcgccgtc
     4021 ggcttcagcg cggcctacgc ctcgcagccg gcacccggca cggcctacga ccagcagcag
     4081 caggcaccga aggactgcaa gatgaagcct gaggatccgc gctgcaagga cgagccgaag
     4141 ggttgagtca gaccaccccc gcagcgcgca gctcatcgcg cgcggcggcg ggcaggccaa
     4201 gcacggtttc cagcacctcg tcggtgtgcg cgccgagcgc cggacccggc gcgtcggtgc
     4261 gccccggcgt ttcgcccagc agcggcggga tcgccggaat cttcagcggc ttgccgccca
     4321 cgctcacggt ctcgaacatg ccgcgcgcgt tgaaatgcgg gtcggccatc atgtcggcga
     4381 cgctgtagat cgggccgttg ggcacctcgg cgtcgcgcag cgcagccagc acctcggcgg
     4441 catcgcgcgc cccggtccag gcggatatcg ccgcatcgat cgcgcgctcg ttctgcaccc
     4501 gcagcgcgtt gctggcgtag cgcgcgtcct cggccatctc cggacggccg atggcgcgca
     4561 tcaggcgctg gtagatggaa tcgccgttgc cgccgatcac cacgtacttg ccgtcggcgc
     4621 agcggtaggt gttggtcggc acgatgccgg tgatggtcga gcccgagggt tcgcgcacta
     4681 cgcccgcgcc gtcgtattcg ggcaccacgc cctccagcat gttgaacacc gattcgtaga
     4741 tggcgacgtc caccacctgg cctgcggccg ccttgccgcg cagccgcgcg gcgagcgcga
     4801 gcaggatgcc gagcaccgcg tgcagcccgg cgagcgtatc gcccatcgag atgttggggc
     4861 gcaccggcgc ctcgccgggc acgccgttca ggtagcggaa gcccgcgaag ccctcgcaca
     4921 ccgaggcgaa gcccggccgc gcggcgtagg gcccggtctg gccgtagccc gagacgcggg
     4981 catagatcag ctccgggcgc aggcgcttga gctcctccgg gccgaggccc cacttttcca
     5041 tggtgccggg gcggaaattc tcgatcagca cgtcggactt ggccgccagc gcgcgcgcca
     5101 gctcgcgccc gcgcgcgctc tgcaggtcga gcgtcaccga cttcttgttg cgcgcgagac
     5161 tcgcccacca gagcgaagtg ccgtcgcgca cctcgcgcca gccgcggatc gggtcgccgc
     5221 gcggcggctc gatcttgatc acctcggcgc cgaaatagcc cagcacgcag ccggcgaatg
     5281 gacccgccag cagctggccc atctccagga cgcggattcc atccagcggt cgtgcggcgg
     5341 cgctgttctg catggtgcga ctataaaccg ggtttcgtcg ctagaatcgc gccttctgcg
     5401 ccaagcagag accccaggag gagatcccca tgacctcacg cagaatcgcc ctcggcgccg
     5461 cacttgcggc cgcggtggtg ctggccacgc cgtcgttcgc gcaggagcgc acgctgcgca
     5521 tcctggtcgg cttcccgcca gggggcacca cggacatcct ggcgaggctc ctggcggagc
     5581 gcctgcgcgc gccgctcggc cagaacgtga tcgtcgagaa caagcccggc gccgtggcct
     5641 cgatcgccgc cgcggaagtg aagaattcgc ccgccgacgg ctcgaccctg ctgatgagcg
     5701 tggtcggcgc gatgaccgtg gcgccgcacg cgcgaaagtc gaatatcttc gacacgctga
     5761 aggactttgc gccggtgtcg ctggtcacca acgcgcacct cgccatcgcg gtgggcccgg
     5821 gctcgcccgc gaaggacctg aagggcttcc tcgcctgggc gaagggcaac cccggcaagg
     5881 cgttctacgc cacctcgggc gccggcaccc tgccgcacat gctcgggctg ctgctggcgc
     5941 aggaagccgg cgtgcaactc acccatgtgc cctacaaggg gacttcggcc tacatcaacg
     6001 agctgatggg tggccaggtg cccgcggcat tcgacgcgat gggagatctc tcggagcagc
     6061 acaaggccgg acgcgtccgc atccttgcca gcgccggcac ggcgcgctcc agggcgatgc
     6121 ccgagatccc gaccatgcgc gagcagggct acaacatcga aggcgccgcc tggttcggcc
     6181 tgttcgcccc ggccgccacg ccgcgcggca ccgtcgagcg cctctcgcag gaggtcgcgc
     6241 gcgtgctgca gggcgccgac atcggcgcca agatcgccag cctcaacatg gagcccgtcg
     6301 gcagcacccc ggaggaattc cagcgcgtgg tgcgcgccga ctgggccaag tggggcgcgg
     6361 tggtgcgcgc ttccggattc accatcgact agcgatggcg caggtccagc catgatccgg
     6421 gcgtgaagcg ggaggcaccg ccggccgacg ccatcgtcat acgcggcgcg cgccagaaca
     6481 acctcaagaa cctgacgctc tcgctgccgg cgaacgagct gatcgtcgtc accggcgtgt
     6541 cgggctcggg gaagtcctcg ctggtgttcg acaccctgta cgccgagggc cagcggcgct
     6601 acgtcgagac cttcagtccc tacgcgcgcc agttcctcga gcgcatggac aagccgcagg
     6661 tggacgccat cgaaggcatc ccgccggcga tcgccatcga ccagaccaac ccggtgcgca
     6721 cgtcgcgctc cacggtcggc acgatgaccg agctgaacga ccacctgaag ctgctgttcg
     6781 cgcgcgggg
//
LOCUS       DASRIE010000061         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1370022, whole genome shotgun sequence.
ACCESSION   DASRIE010000061 DASRIE010000000
VERSION     DASRIE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370022"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFB53_02760"
     CDS             <1..876
                     /locus_tag="VFB53_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN85684.1"
                     /translation="AMSLSLDPIIAATDFSDDAGHAVRRAARLAARHGARLELLHVVD
                     RVALDAVRVWTRNPVDLAERLVDDARRLLGECAAALGSVVAPRVAVGEVQEEILSGCA
                     GAGLLALGAHGVNPLRDAIVGTTAERLLGRCRTPVLVVRQPPRTDYRTALVAVDLLPG
                     SEHALEAAARIAPDADLSALHAYEVPFEGMLQRSGVDQADIDRHRADAFRRAFEDIGR
                     LSAAASGDAERFLPIVERGDAARLCLERAQAIGADLIVIGKRKRPALEALALGSVTRH
                     VLANAKADVLVLPLL"
     gene            complement(856..1149)
                     /gene="mnhG"
                     /locus_tag="VFB53_02765"
     CDS             complement(856..1149)
                     /gene="mnhG"
                     /locus_tag="VFB53_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HZN85685.1"
                     /translation="MTTVAAVLVLAGALLAFLGSLGLLRMKTFFERVHPPTMGTTLGT
                     ALVLAGSMLYFSALESRPVLHEILIAVFMTVTTPVTYLLLGRAALRRSEEGED"
     gene            complement(1146..1427)
                     /locus_tag="VFB53_02770"
     CDS             complement(1146..1427)
                     /locus_tag="VFB53_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HZN85686.1"
                     /translation="MNPELFVAVLAAAQLLLALGIAFAFCRLLRGPRAHDRVLALDTL
                     YVNAMLLILSFGMVWASRDFFEVALVIGALGFVSTAAAAKFLLRGEVIE"
     gene            complement(1424..1894)
                     /locus_tag="VFB53_02775"
     CDS             complement(1424..1894)
                     /locus_tag="VFB53_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HZN85687.1"
                     /translation="MRLFLALLALWLILNESLAPGQVLVGVLLALGGSAVFARLQPPT
                     GRVRSRPGAALQLLGLVLADIVRSNLAVARIVLGLGAARRKAGFLALPLELRHPAGLA
                     VVACIITATPGTSWVRYDSAANVVTIHVLDLLDADDWIRLFKQRYERRLLEIFG"
     gene            complement(1891..>2504)
                     /locus_tag="VFB53_02780"
     CDS             complement(1891..>2504)
                     /locus_tag="VFB53_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899926.1"
                     /note="subunit D of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; contains an oxidoreductase domain; catalyzes the
                     transfer of electrons from NADH to ubiquinone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN85688.1"
                     /translation="AISRGDGAVTAAALFYLVASTLGIAAFFLLIELVQRAREPGADV
                     LAVTAEAFGLGDEDVEPEERPGTVIPAAMAVLGLSFLCCALLVASLPPLPGFLAKFAL
                     LAAVLGPQPVGAPAWALIALLTIAGLAGIIAMGRAGVRIFWAAEERSTPRVRVLEIAP
                     VAGLLALCFVLTLGAGPVMRYLQDAALALHAPQTYIESVLSRP"
BASE COUNT          400 a          897 c          916 g          291 t
ORIGIN      
        1 gccatgagcc tgagcctcga cccgatcatt gcggcgaccg atttctcgga cgatgccggc
       61 cacgcggtgc gtcgggccgc gcggcttgcc gcccggcacg gtgccaggct cgagctgctg
      121 cacgtggtgg accgcgtggc gctggatgcg gtgcgcgtct ggacgcgcaa cccggtggat
      181 ctcgccgaac ggctggtcga cgatgcccgc cgcctgctcg gcgaatgcgc ggccgcgctc
      241 ggcagcgtgg tcgcgccgcg cgtcgcggtg ggcgaggtgc aggaggagat cctttcgggc
      301 tgcgccggag ccggcctgct ggcgctgggc gcgcacgggg tcaatccgtt gcgcgacgcc
      361 atcgtcggca ccaccgccga gcggctgctg ggcaggtgcc ggactccggt gctggtggtg
      421 cgccagccgc cgagaacgga ctaccgcacg gccctggtcg cggtcgatct gctgccgggg
      481 tcggagcacg cgctcgaggc ggccgcgcgc atcgcgccgg acgccgacct gagcgcgctc
      541 cacgcctacg aggttccctt cgagggcatg ctgcagcgct ccggcgtcga tcaggccgac
      601 atcgaccgcc atcgcgccga cgcctttcgc cgcgccttcg aggatatcgg ccggctgagc
      661 gcggcggcga gcggcgatgc cgagcgcttc ctgccgatcg tcgagcgcgg cgatgccgct
      721 cggctgtgcc tggagcgcgc gcaggcgatc ggcgccgacc tgatcgtcat cggcaagcgc
      781 aagcgccccg cgctggaggc gctcgcgctc ggcagcgtca cgcgccacgt cctggcgaac
      841 gcgaaggccg acgtgctagt cctccccctc ctctgaacgt cgcagcgcgg ccctgccgag
      901 cagcaggtag gtgaccggcg tggtgacggt catgaacacg gcgatcagga tttcatgcag
      961 caccgggcgc gactcgagcg cgctgaagta aagcatcgag ccggccagga ccagcgccgt
     1021 gccgagcgtg gtgcccatgg tcggcggatg cacgcgctcg aagaaggtct tcatccgcag
     1081 caggccgagc gagccgagga aggcgagcag cgcgccggcg agcaccagta cggcggcgac
     1141 ggtagtcatt cgattacctc gccgcgcagc aggaacttgg ccgccgcggc ggtcgatacg
     1201 aagccgagcg cgccgatcac cagcgcgacc tcgaagaagt cgcggctcgc ccagaccatg
     1261 ccgaagctga ggatgagcag catcgcattc acgtagagcg tatcgagcgc gagcacgcgg
     1321 tcgtgcgcgc gcggcccgcg cagcaggcgg cagaaggcga acgcgatgcc gagcgcgagc
     1381 agcagctgcg cggccgcgag caccgcgacg aacagctcgg ggttcatccg aagatctcca
     1441 gcagccggcg ctcgtagcgc tgcttgaaca ggcgaatcca gtcatccgcg tcgagcaggt
     1501 cgagcacatg gatcgtgacc acgttggcgg cgctgtcgta gcgcacccag ctcgtgcccg
     1561 gggtggcggt gatgatgcag gccaccacgg ccagccccgc gggatgccgc agctcgagcg
     1621 gcaacgcgag gaagccggcc ttgcgccgcg ccgcgcccaa cccgaggacg atgcgcgcca
     1681 cggcgaggtt ggagcgcacg atgtccgcga gcaccaggcc gaggagctgc agcgccgcgc
     1741 cgggacggct gcgcacccgg ccggtgggcg gttgcaggcg cgcgaacacc gcgctgccgc
     1801 cgagcgcgag caggacgccg accagcacct gccccggggc gagcgactcg ttgaggatca
     1861 gccaaagcgc gagcagggcg aggaacagcc tcatgggcgc gaaagaacgc tttcgatgta
     1921 ggtctgcggg gcgtgcagcg cgagcgccgc gtcctgcaaa tatcgcatca ccgggcccgc
     1981 gccgagcgtg agcacgaagc acaacgccag cagcccggcc accggcgcga tctccagcac
     2041 ccgcacgcgc ggcgtgctgc gctcctccgc ggcccagaaa atgcgcacgc ccgcccgtcc
     2101 catggcgatg atcccggcca ggccggcgat cgtaagcagg gcgatcagcg cccacgcggg
     2161 cgcgccgacc ggctgcggcc cgagcaccgc ggccagcagc gcgaacttcg ccaggaagcc
     2221 cggcagcggt ggcaggctgg cgacgagcag cgcgcagcaa agaaagctca ggccgagcac
     2281 cgccatggcg gccggaatca ccgtgccggg ccgctcctcc ggctccacgt cctcgtcgcc
     2341 gaggccgaag gcctccgcgg tcaccgccag cacgtcggcg ccgggctcgc gcgcgcgctg
     2401 caccagctcg atcagcagaa agaacgccgc gatcccgagc gtcgaggcca ccaggtagaa
     2461 cagcgcggcg gcggtcaccg cgccgtcgcc gcggctgatc gccg
//
LOCUS       DASRIE010000062         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2684365, whole genome shotgun sequence.
ACCESSION   DASRIE010000062 DASRIE010000000
VERSION     DASRIE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684365"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFB53_02785"
     CDS             complement(<1..388)
                     /locus_tag="VFB53_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN85689.1"
                     /translation="MPPSEFMVRFWGVRGSIACSGPRTVRYGGNTSSIEVRCGERLLL
                     FDGGTGLRYLGNTLAGPIDADLFLTHTHFDHVCGLPFFRPFFDPKNRFRLWAGHLADG
                     MTLRRVLGEFMMAPLFPVPPQVFRARM"
     gene            430..3063
                     /locus_tag="VFB53_02790"
     CDS             430..3063
                     /locus_tag="VFB53_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN85690.1"
                     /translation="MDRSLFGFIIRYSKRDQLMIVPLVAATMVVYFLTLDLPKTIINK
                     AIQGGAFPQPDATARFLGFDLPRIEYLFALSIVFLGLIVLNGWLKFQINTMKGWMGER
                     MLRRLRYALYDFILRFPLARFRRVKGAEMASMIKDEVEPLGGFIGEALITPLFLGGQA
                     LTAMFFILYQHWALGLIALAVVSVQAGVIPKLRKRLLVLGRERQLTARALAGRIAETV
                     DGAAEIHANGTSNYERAEISARLGRIFRIRFELYQRKFMVKFLNNFLSQVTPFLFYTL
                     GGYLVIVGRLDIGALVAVIAAYKDLPGPVKELIDWDQQRMDVEGKYQGVVEQFTGEEV
                     APPELQALVEVPPLPREGRLKVSNLSLVDESGQRLVDNVSFEAGLDQHVAILGKQGSG
                     AGELAQLLARLMPPSAGSVELCGLDLTRAPQALTGRAVGYVGASAYLFPARVRDNVVY
                     ALKHRPIQEKIYEKEAVAAREFQLREARRTGNSELDIDAEWIDYAAAGCAGPDELDAR
                     IVDLLRVVDLEETIFELGLRSPADVSRNQDVPGKVLAARGSMRERLESLGIKDWVERW
                     DPAHYNRNATLAENLLFGTPIGRVFDVENLAANAYVRQVLRETGLHELLVRTGHKVAE
                     TMVELFSGLPPGHEFFAQYSFINQDDLPQFEAILKRVTDVGLKGLDEAEGRALLALPF
                     KLIAARHRLGLIDEGFEGRVIEARRHFAAGLPAGLKGAVEFFDPARYNSAATLQDNIL
                     FGKIVTGQAGAGSRITTLVRELLDELGLRPLVVKIGLDFQVGVGGARLAPADRQKIAL
                     VRALLKQPLLLVLDQAVAVLDPTSQQRVIEGVLAERKGRGLAWVLSRPEYAQRFAIVL
                     SMDHGRLAASGSPVDLKRKTG"
     gene            3060..3518
                     /locus_tag="VFB53_02795"
     CDS             3060..3518
                     /locus_tag="VFB53_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN85691.1"
                     /translation="MSLEQEVELIRQFPIFARIQPAMQKLLCFSAERLTYAPGQVMFK
                     AGDMGDAAYIVIEGSVEISVPTPAGPIVVNTLGKNDIIGEIAIFGDVPRTATATAQTK
                     LETLKISKELFTKIIRENPDAALELIKVLAGRLANTTQQLSRTLSGTTRP"
     gene            complement(3483..>4802)
                     /locus_tag="VFB53_02800"
     CDS             complement(3483..>4802)
                     /locus_tag="VFB53_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN85692.1"
                     /translation="ERVVGVLWANPWTTFKQTALLLIVWAHVCVGLHYWLRLRAWYRD
                     LEPFVFALALLVPTLALAGFASAGFYLWPSIESVGGIHKYNLDLAGMSRQDRALLAAW
                     SNGLEYGFWIALAATLLARWLRTRIGATYQMRHASGRVITAPVGRSLLEAIRDEGLPH
                     ASICGGRARCTTCRVRVGDGLAHLPPPGRLEAQALARIEAPPNVRLACQCRPMRDVTV
                     TPLLPPNAGPRQAGGDRGAQGRERAIVAMFVDLRDSTRLAESRLPYDTVFLMNQFFAE
                     MHQALRATGGYYAQFRGDGLLALYGLESSLAEACRAALRGAAEMQDRIERLNASLAAD
                     LEAPLRIGIGIHAGVAIVGTLGPPDAPIYSAIGDMVNTAARLEDMTKAYTCTLVVSAE
                     ALRQAGVEPGGAPLHQVRVRGRDERIDVYAVGDPRALRARGAAESAG"
BASE COUNT          763 a         1659 c         1689 g          691 t
ORIGIN      
        1 ccatgcgcgc gcggaacacc tgcggcggca ccgggaacag cggcgccatc atgaactcgc
       61 ccagcacgcg gcgcagcgtc atgccgtcgg cgaggtgccc ggcccacagg cgaaagcggt
      121 tctttgggtc gaagaacggg cggaagaagg gcaggccgca gacgtggtcg aaatgggtat
      181 gcgtgagaaa caggtcggcg tcgatcggtc ccgccagcgt attgccgagg tagcgcaggc
      241 cggtgccgcc atcgaacagc agcaggcgct cgccgcagcg cacctcgatc gaggaggtgt
      301 tgccgccata gcgcaccgtg cgcggccccg agcaggcgat ggaaccgcgc acgccccaga
      361 agcgcaccat gaattcggac ggcggcatcc ttgaactata gcgataagat gtgccctagg
      421 ggagacgcga tggaccgcag tctgttcggc ttcatcatcc gctacagcaa gcgcgatcag
      481 ctgatgatcg tgccgctggt ggccgcgacc atggtggtgt acttcctcac cctcgatctg
      541 ccgaagacca tcatcaacaa ggcgatccag ggcggcgcct tcccgcagcc cgacgccacc
      601 gcgcgcttcc tcggcttcga cctgccgcgc atcgagtacc tgttcgcgct cagcatcgtg
      661 ttcctcgggc tgatcgtgct caacggctgg ctcaagttcc agatcaacac catgaagggc
      721 tggatgggcg agcgcatgct gcgccgcctg cgctacgcgc tgtacgactt catcctgcgc
      781 ttcccgctgg cgcgcttccg gcgcgtcaag ggcgcggaga tggcgagcat gatcaaggac
      841 gaggtcgagc cgctcggcgg cttcatcggc gaggcgctga tcacgccgct gttcctcggc
      901 ggccaggcgc tcacggcgat gttcttcatc ctctaccagc actgggcgct cggcctgatc
      961 gcgctcgcgg tggtctcggt ccaggccggg gtgatcccga agctgcgcaa gcgcctgctg
     1021 gtgctcggcc gcgagcgcca gctgaccgcc cgggcgctcg ccgggcgcat cgccgagacc
     1081 gtcgacggcg ccgccgagat ccacgccaac ggcacctcga actacgagcg cgccgagatc
     1141 tcggcacgcc tcggcaggat ctttcgcatc cgcttcgagc tgtaccagcg caagttcatg
     1201 gtgaagttcc tgaacaactt cctgtcgcag gtgacgccgt tcctgttcta taccctgggc
     1261 ggctacctgg tgatcgtcgg caggctcgac atcggcgcgc tcgtggcagt catcgccgcc
     1321 tacaaggacc tgcccggccc ggtgaaggag ctgatcgact gggaccagca gcgcatggac
     1381 gtcgagggca agtaccaggg cgtggtcgag cagttcaccg gcgaggaagt ggcgccgccg
     1441 gagctgcagg cgctggtcga ggtgccgccg ctgccgcgcg agggcaggct caaggtgtcc
     1501 aacctctcgc tggtcgacga atcgggccag cgcctggtgg acaacgtgtc cttcgaggcc
     1561 gggctcgacc agcacgtggc gatcctcggc aagcagggct cgggcgccgg cgagctggcg
     1621 cagctgctgg cgcggctgat gccgccgagc gccggctcgg tcgagctgtg cggcctcgac
     1681 ctgacgcgcg cgccgcaggc gcttaccggg cgcgccgtcg gctacgtcgg cgcgtcggcc
     1741 tatctgttcc cggcacgggt gcgcgacaac gtggtctacg ccttgaagca tcgccccatc
     1801 caggaaaaga tctacgaaaa ggaagcggtc gccgcgcgcg agttccagct gcgcgaagcg
     1861 cggcgcaccg gcaacagcga gctcgacatc gacgccgagt ggatcgacta cgcggccgcg
     1921 ggctgcgccg ggcccgacga gctcgatgcg cgcatcgtcg atctgctgcg cgtggtcgac
     1981 ctggaggaga ccattttcga gctcggcctg cgcagccctg ccgacgtctc ccgcaaccag
     2041 gatgttcccg ggaaggtact cgcggcccgc ggcagcatgc gcgagcggct ggagtcgctc
     2101 ggcatcaagg actgggtcga gcgctgggac ccggcgcact acaaccgcaa cgccacgctc
     2161 gccgagaacc tgctgttcgg cacgccgatc gggcgcgtct tcgacgtcga gaacctggcc
     2221 gccaacgcct acgtgcgcca ggtgctgcgc gagaccgggc tgcacgagct gctggtgcgc
     2281 accgggcaca aggtcgccga gaccatggtc gagctgttca gcgggctgcc gccgggtcac
     2341 gagttcttcg cccagtacag cttcatcaac caggacgacc tgccgcagtt cgaggcgatc
     2401 ctgaagcgcg tcaccgacgt cggactgaag ggcctcgacg aggccgaggg ccgggcgctg
     2461 ctcgcgctgc cgttcaagct gatcgccgcg cgccaccgcc tgggcctgat cgacgagggc
     2521 ttcgaggggc gcgtgatcga ggcgcggcgc catttcgccg ccggcctgcc cgccgggctc
     2581 aagggcgcgg tggagttctt cgatccggcg cgctataact cggcggcgac gctgcaggac
     2641 aacatcctgt tcggcaagat cgtcaccggg caggcgggcg cggggtcgcg cattactacc
     2701 ctggtgcgcg agctgctcga cgagctcggc ctgcgcccgc tggtagtgaa gatcggcctg
     2761 gacttccagg tcggcgtcgg cggcgcgcgc ctcgctccgg ccgaccggca gaaaatcgcg
     2821 ctggtgcgcg cgctgctcaa gcagccgctg ctgctggtgc tcgaccaggc ggtagcggtg
     2881 ctcgacccga cctcgcagca gcgggtgatc gagggggtgc tcgccgagcg caagggccgc
     2941 ggccttgcct gggtgctgtc gcgtcccgaa tacgcccagc gcttcgcaat cgtgctgtcg
     3001 atggaccacg ggcgcctcgc cgcgagcggc agcccggtgg atctgaaaag gaaaaccgga
     3061 tgagcctgga acaggaagtc gagctgatca ggcagttccc gatcttcgcc aggatccagc
     3121 cggcgatgca gaagctgctg tgcttcagcg ccgagcgcct cacctacgcc cccggccagg
     3181 tgatgttcaa ggccggcgac atgggcgacg ccgcctacat cgtgatcgag ggaagcgtcg
     3241 agatcagcgt gccgacgccc gccggcccga tcgtcgtcaa caccctgggc aagaacgaca
     3301 tcatcggcga gatcgcgatc ttcggcgacg tgccgcgcac cgccaccgcc accgcgcaga
     3361 ccaagctcga gacgctgaag atctccaagg agttgttcac caagatcatc cgcgagaacc
     3421 cggacgccgc gctggagctg atcaaggtgc tcgccgggcg gctcgccaac accacgcagc
     3481 agctatcccg cactctcagc ggcaccacgc gcccgtaggg cgcgcggatc gccgacggcg
     3541 tagacgtcga tgcgctcgtc gcgcccgcgc acccgcacct ggtgcagggg tgcgcccccg
     3601 ggctcgacgc ccgcctgccg cagcgcctcg gccgacacca ccagcgtgca ggtgtacgcc
     3661 ttggtcatgt cctcgagccg cgccgcggtg ttcaccatgt cgccgatcgc cgagtagatc
     3721 ggcgcgtcgg gcggtcccag ggtgccgacg atcgcgaccc cggcgtggat gccgatgccg
     3781 atgcgcagcg gcgcctcgag atcggcggcc aggctggcgt tgagccgctc gatgcggtcc
     3841 tgcatctcgg ccgcgccccg gagcgcggcg cggcacgctt cggccaggga tgattcgaga
     3901 ccatagaggg cgagcaggcc gtcgccgcgg aactgggcgt aatagccgcc ggtcgcgcgc
     3961 agcgcctggt gcatctcggc gaagaactgg ttcatcagga acaccgtgtc gtagggcagg
     4021 cggctctcgg cgaggcgggt cgagtcgcgc aggtcgacga acatcgccac gatcgcgcgc
     4081 tcgcgcccct gcgcgccgcg atcgccgccg gcctggcgcg gcccggcgtt cggcggcagc
     4141 agcggcgtca ccgtgacgtc gcgcatcggg cggcactggc aggcgaggcg cacgttcggc
     4201 ggcgcctcga tgcgcgcgag cgcctgcgcc tccaggcgcc cgggcggcgg caggtgcgcg
     4261 aggccgtcgc cgacgcgcac gcggcaggtg gtgcagcgcg cgcgcccgcc gcagatcgag
     4321 gcgtgcggca gtccctcgtc gcggatcgcc tcgaggaggc tgcgcccgac cggcgcggtg
     4381 atcacccgcc ctgaggcgtg gcgcatctgg taggtggcgc cgatgcgcgt gcgcagccag
     4441 cgcgcgagca gcgtcgcagc gagcgctatc cagaacccgt actcgagccc gttgctccag
     4501 gcggcgagca gcgcgcgatc ctgccgactc atgccggcca ggtcgaggtt gtacttatgg
     4561 atgccgccga cgctctcgat gctcggccag agatagaagc cggcgctggc gaagcccgcc
     4621 agggcgagcg tgggtaccag cagcgcgagc gcgaagacga acggctccag gtcgcggtac
     4681 caggcgcgca gccgcagcca gtagtgcagg ccgacgcaga cgtgcgccca cacgatgagg
     4741 agcagcgcgg tctgcttgaa cgtggtccac gggttcgccc acagcacgcc gacgacgcgc
     4801 tc
//
LOCUS       DASRIE010000063        13803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3729893, whole genome shotgun sequence.
ACCESSION   DASRIE010000063 DASRIE010000000
VERSION     DASRIE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729893"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFB53_02805"
     CDS             <1..813
                     /locus_tag="VFB53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZN85693.1"
                     /translation="DAAKPATFEVEAVNERGEMVPTAVAGEHPLTLYLDKRELVTLMT
                     LGHAPEALAIGYLRNQRLVGALEDITAVQVDWETGSCAVTTRKAVKDLNRKLGKRTVT
                     SGCGQGTMFGSLMEEIGEVRLRQDVTLADEQLFALLDRVRKHETIYKQAGAVHGCALA
                     TRAGEILMFVEDVGRHNAVDAIAGRMWLEAIDGSDKLFYTTGRLTSEMVIKCAQMAIP
                     FLVSRSGLTQMGYEIAQQTGITMLGRASGRHYLAFTGRERLLRSAPQPTAIA"
     gene            823..1482
                     /locus_tag="VFB53_02810"
     CDS             823..1482
                     /locus_tag="VFB53_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN85694.1"
                     /translation="MIELYFAPGACSFVPHVGLEAIKDATGENFEPKLVKLHKGENKT
                     PEYLAMNPNGQVPVLVVDGKPLNQIVAICDFLDRRFPPAGLLPAEPWARAQALSQLAW
                     FNNTVHTSFTRWFRTGYFAESEAGQADVKKLAVADLRRHLERIQEWSKAAAPFWFGAR
                     PSFHDAYAFTLLRWGGYAGIDPESLPAYKAYLERVVQAPAVAAALARERIALDTYKKA
                     A"
     gene            complement(1479..2675)
                     /locus_tag="VFB53_02815"
     CDS             complement(1479..2675)
                     /locus_tag="VFB53_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85695.1"
                     /translation="MTQNRWLVPFLNLGHLLDHLVMLVFPTAVVALGREWGRPYSELL
                     PLALGGFIAFGAFAIPAGWLADHWSRYRTLAIFYFGIGVSLFLTGLSQSPWHIAAGLT
                     LTGAFAAIYHPVGIAMLVANPQNMGRTLGWNGLWGNLGLAAAALFTGALVDFAGWRAA
                     FFVPGLICVAAGAGFLLLVKDTGKVRKSSKTIGLHVEGRTMARIFAILLIATACGGVI
                     FNSTTVAMPKVFDERLQALTQTNFGIGVLVAVVYTVAAFAQVAMGALIDRFELKRLLV
                     GVALAQIPLLALAANTQGWAMLGAAVLMMLAVFGQIPLNDAIVGRYCADEYRARVLSV
                     RYVVSLGVAAVAVPMIAVLHRTQGGFANVFLVLAALASAMLLASLFFPSRGVLAASKQ
                     RAAQPV"
     gene            2708..3271
                     /gene="mobA"
                     /locus_tag="VFB53_02820"
     CDS             2708..3271
                     /gene="mobA"
                     /locus_tag="VFB53_02820"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HZN85696.1"
                     /translation="MDSVSGIVLAGGQGRRMGGVDKGLVPLRGKPMVAWVLERLAPQV
                     DGILVNANQNAEAYAAFGHPVVPDAIGGFAGPLAGLHAGLSAASTPLVVTVPCDSPFL
                     PLDLVARLRKALGDNDLAVARTGDQPHPVFSLVRRAVRAHLESYLAGGGRKIDAWYAT
                     LKVVEVPFDDEAGAFRNINTREELKDA"
     gene            3268..4527
                     /gene="glp"
                     /locus_tag="VFB53_02825"
     CDS             3268..4527
                     /gene="glp"
                     /locus_tag="VFB53_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZN85697.1"
                     /translation="MSRIVQIVSAIEGYDPDALHVEKARAAIRACLAPVTQSERVPVR
                     AALGRVLAEDIVPAIDVPGHDNTAMDGYAVRFADLGAGETVLEEIGAALAGRSFGGKL
                     AAGQCVRVTTGAVMPAGADTVVVQEVVKVNGARVTIPPGQKKSQNVRYAGEDLQKGKP
                     VLAPGKALGTAELGLVASLGIGEIAVRRRLRVAFFSTGDELASVGGTLREGEVYDSNR
                     YTLHGMLARLGVEMHDLGVVRDDPALLEAAFRKAADAADAIVTTGGVSVGEADFVKPM
                     MAKLGEALFWRIAMRPGRPMAFGRIGKAFLFGLPGNPVAVMVTFTQFVREALLQLGGR
                     TGELSVPLLKVPAAEPLRKVAGRTEYQRGILFRDGGDWKVKTTGQQGSGVLRSMSEAN
                     CLIVLEHERGTVAAGEPVQVQLFEGLL"
     gene            4524..4985
                     /locus_tag="VFB53_02830"
     CDS             4524..4985
                     /locus_tag="VFB53_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HZN85698.1"
                     /translation="MNPIHPSLAPLAVIAKKDPHLLHEHLRAALSDPAWLRPEHRVGD
                     PHAYMRHLLYVDPDGDFVITAITWLPGQQSPMHGHHVWCAFGVAEGELTEERFDEQGV
                     LQRTVRYAAGDVAPSSPIHRVANRGRAPLVSLHLYGVSAERLTTGINRIYA"
     gene            complement(4976..5800)
                     /locus_tag="VFB53_02835"
     CDS             complement(4976..5800)
                     /locus_tag="VFB53_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN85699.1"
                     /translation="MRLGVDLGGSKIEAAVLDAQGKVALRRRVPTPRGAYEDALRLLQ
                     TQIAELEREAQEPCTVGIGLPGTLSRRTGLVANAYNTPVNGRPLKRDLEAALGRELRF
                     ANDANCFALSEARDGAARGARVVFGAVLGTGAGGGLVVDGRALEGHHGLAGEWGHNPL
                     PWMRPEEYPGPVCSCGRAGCIEQFVSGTALRRDGASAALHADRLARALAQVVNLLDPD
                     AIVLGGGVSNIASLYDQLPPLIARYAYTDAFSTPVLRAAHGDASGVRGAAMLWDQA"
     gene            complement(5800..7590)
                     /locus_tag="VFB53_02840"
     CDS             complement(5800..7590)
                     /locus_tag="VFB53_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZN85700.1"
                     /translation="MSQTLDLAIVGNANIAALIDAKGEVVWACLPRMDGDPVACSLLR
                     ERKGADDFGFFAFELADFSHSEQAYLPNTPILVTRLYDRHGGAVEITDFVPRFRHHAR
                     IFSPMTLVRELRPLAGSPRVRVLLRPASQYGAKRPALTYGSNHVRYVAENYVMRVTTD
                     CSITALVDELAFVLLRPLAFILGPDETLQGGVAETARRFHEETADYWREWVRDLQIPF
                     EWQDEIIRAALTLKLSAVDDTGALVAAVTTSIPEAKASGRNWDYRYCWLRDAYFTINA
                     LNRLNATRTMERYLGYIINVAAGAEGARLQPVYRINGAAQLEEREIATLAGYRGMGPV
                     RVGNQAYLQVQNDVYGSAVLAATHAFFDQRLMRSGNEALFRHLEVLGERAVAVWNEPD
                     AGLWELRGAGRVHTFSSVMCWAACDRLAKIAARLGLAPRAAYWQAHAQRIHGGICERA
                     WSARRNSFAATFGGEGLDASLLLLAELRFLPADDPRYRATLAAVERELKKGDFVFRYT
                     EPDDFGAPENAFLVCSFWFVDALAAVGRRDEARALFQDLLGCRNRHGLLAEHVDPATR
                     ELWGNFPQTYSMVGLVNSATRLSIGWDEAF"
     gene            complement(7587..8336)
                     /gene="otsB"
                     /locus_tag="VFB53_02845"
     CDS             complement(7587..8336)
                     /gene="otsB"
                     /locus_tag="VFB53_02845"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZN85701.1"
                     /translation="MSAHGQRALPPVTDWALFLDVDGTLLEHAARPDAVRVAAPVHQL
                     LEALRLATRGALALISGRAIDDIDALFAPLRLPVAGQHGAERRDAAGRLHRHQGAAEP
                     VRRAAARLGAFAARHAGLVFEDKGHNLALHYRQAPAAAQAAQAALRAAALELGGDYEV
                     LEGKMVVELKPAGRDKGRAIAEFMAEAPFAGRTPVFIGDDVTDEFGFGVVNRLDGHSV
                     KVGPGASAARHRLADAAAVREQLAQWAAQRA"
     gene            complement(8333..10576)
                     /locus_tag="VFB53_02850"
     CDS             complement(8333..10576)
                     /locus_tag="VFB53_02850"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZN85702.1"
                     /translation="MRLSLRFIVPLGLALAAIAYAVVPLVDRLTLQWFERDLEMRATT
                     IANAAQERLAALVREPDARPKVMSYFEHIIQDERIFALGFCERDGKLPFATGTFPGSQ
                     LCASTGREAYTGRLQHTQGPLHVAAIPILADREVLGDLVIVHDMSFVTRRSDDTKKYV
                     VWLFAAIAAVVALITVVIAEISWRGWVAGMKRIISGEALLGTREGLRVTAPELRPIAR
                     DLQALVRDLEAERHVRDETQMTWGPEALRGVLREDLKGDEVLIVSNREPYIHVRRKDN
                     VIEIQRPASGLVTALEPVMRACSGTWIAHGAGSADRDTVDQGDYVMVPPDKPAYRIRR
                     VWLSKAEEQGYYYGFANEGLWPLCHIAHTRPVFRADDWEHYKAVNQRFAKVVVDEART
                     EDPIILVQDYHFALLPRMVRERLPRATIIAFWHIPWPNPEAFGILPWREEVLEGLLGS
                     SIVGFHTQFHCNNFFDTVDRFLETRVDRETFTISYGGAPTEVRRYPISIEWPPTALAA
                     QAPIAEARRALRARLGLAAEVALGVGVDRLDYTKGILERFMAVERLLELEPRWVGKFA
                     FVQAAAPSRSSIDEYRNLEARVRELAARVNRRFGSAGYEPIVLRIEHHDAAQVYELYR
                     AADFCCVSSLHDGMNLVAKEFVAARDDQQGVLILSQFTGAARELVEALTVNPYDIEQS
                     AAAMHLALTMPPAEQRARMRSMRTLVQDFNVYRWAGRMLLDAARMRHRRRVLSRARAA
                     RREAGTG"
     gene            complement(10670..11641)
                     /locus_tag="VFB53_02855"
     CDS             complement(10670..11641)
                     /locus_tag="VFB53_02855"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZN85703.1"
                     /translation="MKRLAVLTSGGDAPGMNAALRAVTRAALARGWETYGVRNGYAGL
                     LADTLEPLAARDVGGIVQRGGTFLGSARCPEFIETPGRERALRNLAARRIDALVVIGG
                     NGSQTGTAALAREGFAAVGVPSTIDNDLYGTDISIGADTAINVTLEAIDRLRTTASSH
                     QRAFVVETMGRDCGYIALLAGIAGGAEVIAIPEREVEPAEIAERLRAAYRRGKTHALV
                     VMAEGARHGVQALLKHYGEEKDTIGFELRLTRLGHVVRGGVPTAADRVLATRLGAAAV
                     ESLAAGRSGVLMGQQRGEISATALAEIGGRTRPADAALLDLARTMAI"
     gene            complement(11643..12737)
                     /locus_tag="VFB53_02860"
     CDS             complement(11643..12737)
                     /locus_tag="VFB53_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZN85704.1"
                     /translation="MLESLSIIALLVALGALAAVVWLALRLQGGAERKHREMLTDLHD
                     GLVKQGDRLGAQLGELREAVAAKLDARLDQISLRVNERLDEGFRKTNDTFVNVMQRLA
                     TIDEAQKKIETLTGSVVSLQELLGDKRSRGAFGEVQLEALVRNVLPNQAFEMQYTLSN
                     GARVDCVLRLPAPTGLVCVDSKFPLENYKLMLDAKAGEADRAIAQKAFRLDVRKHVDA
                     IASKYIIDNETSDGAVMFVPAEAVFAEIHAYHPEVVDYAIGKRVWIVSPTTLMAVLNT
                     ARAVLKDVETRKQIHVIKEALGRLAVEFGRFDDRLKKLADHIRLAHEDAERIQVTGGK
                     IAQQFQRIESAELEERPPAEIVRLEEGRKS"
     gene            complement(12765..>13803)
                     /locus_tag="VFB53_02865"
     CDS             complement(12765..>13803)
                     /locus_tag="VFB53_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85705.1"
                     /translation="IQSALHEIGLMLARAPGVEPAVLGSLRILCDKLDWETARYWRAD
                     EAGARLAAVAVYPAGAEAASSVLGADQLAALPVRAWRERRAVWGAADGHAFAMPVTAG
                     ELLLGVIELRGSAPGEPEHELLEMATSLGALLGEYIFRTRGDERTRESEARMRGLSRR
                     LLDAQEAERRHVGTELRDMVARPLAAALADSGLARLKAPLAAVRELVAQLRPAALEDY
                     GLLAALRTHAVRFERRTGIALAVIGAEDGAEIEPRLETALFQIARDALDNVARHSGAR
                     NAAVELSVGGGVVLLSIRDDGAGFDTQAARPSDALGLTLMRERAAAIGGQLRVESAPG
                     SGTRVSVRLRG"
BASE COUNT         2051 a         4972 c         4838 g         1942 t
ORIGIN      
        1 gacgcggcga agccggcgac cttcgaggtc gaggcggtca acgagcgcgg cgagatggtg
       61 ccgaccgcgg tggccggcga gcacccgctc acgctgtacc tcgacaagcg ggaactggtc
      121 acgctgatga cgctcggcca cgcgcccgag gcgctcgcca tcggctacct gcgcaaccag
      181 cgcctcgtcg gcgcgctgga ggacatcacg gcggtgcagg ttgactggga gaccgggtcc
      241 tgcgccgtca ccacgcgcaa ggcggtcaag gacctgaaca ggaagctcgg caagcgcacg
      301 gtcaccagcg gttgcggcca gggcaccatg ttcggcagcc tgatggagga gatcggcgag
      361 gtgcggctgc gccaggacgt cacgctcgcc gacgagcagc tgttcgcgct cctcgacagg
      421 gtgcgcaagc acgagaccat ctacaagcag gcgggcgcgg tgcacggctg cgcgctcgcc
      481 acgcgcgcgg gcgagatcct gatgttcgtc gaggacgtcg gccggcacaa cgcggtggac
      541 gcgatcgccg gccgcatgtg gctggaggcg atcgacggct cggacaagct gttctacacc
      601 accgggcgcc tgacctccga gatggtgatc aagtgcgcgc agatggcgat cccgttcctc
      661 gtgtcgcgct cgggcctcac gcagatgggc tacgagatcg cgcagcagac cggcatcacc
      721 atgctggggc gcgccagcgg ccggcactac ctcgccttca ccgggcgcga gcgcctgctg
      781 cgcagcgcgc cgcaacctac cgccatcgcc taggagaagg aaatgatcga gttgtatttc
      841 gcccccggcg cctgttcctt cgtgccgcac gtcggcctgg aggcgatcaa ggacgcgacc
      901 ggggagaact tcgagccgaa actcgtcaag ctgcacaagg gcgagaacaa gaccccggaa
      961 tacctcgcca tgaaccccaa cggccaggtg ccggtgctgg tggtggacgg aaagccgctc
     1021 aaccagatcg tcgccatctg cgatttcctc gaccggcgct tcccgccggc gggcctgctg
     1081 cccgccgaac cctgggcgcg cgcccaggcg ctgtcgcagc tcgcgtggtt caacaacacc
     1141 gtgcatacca gcttcacgcg ctggttccgc accggctatt tcgccgagag cgaagccggc
     1201 caggccgatg tgaagaagct cgcggtggcg gacctgcgcc ggcacctgga gcgcatccag
     1261 gaatggtcca aagcggcggc gcccttctgg ttcggcgcgc gcccgagctt ccacgacgcc
     1321 tacgcgttca cgctgctgcg ctggggcggc tacgccggga tcgacccgga atcgctgccc
     1381 gcctacaagg cgtacctcga gcgcgtggtg caggcgcctg cggtggccgc cgcgctcgcg
     1441 cgcgagcgca tcgccctcga cacctacaag aaagccgcct agaccggctg ggcggcgcgc
     1501 tgcttgctcg cggcgagcac gccgcgcgag gggaagaaca ggctcgccag cagcatcgcc
     1561 gaggcgagcg ccgccagcac caggaacacg ttggcgaagc cgccctgcgt gcggtgcagg
     1621 accgcgatca tcggcacggc caccgcggcg acgccgagcg acacgacgta gcgcaccgac
     1681 agcacgcgcg cgcggtactc gtcggcgcag tagcggccga cgatggcgtc gttcagcggg
     1741 atctgcccga acaccgccag catcatcagc accgccgcgc cgagcatcgc ccagccctgc
     1801 gtgttggcgg cgagcgccag cagcgggatc tgcgccagcg ccacgccgac cagcaggcgc
     1861 ttgagctcga agcggtcgat cagcgcgccc atcgccacct gcgcgaaggc cgccaccgtg
     1921 tagacgacgg ccaccagcac gccgatgccg aaattggtct gcgtcagcgc ctgcagccgc
     1981 tcgtcgaaca ccttgggcat cgccaccgtg gtggagttga agatcacgcc gccgcaggcg
     2041 gtggcgatga gcaggatggc gaagatgcgc gccatggtgc gcccctccac gtgcaggccg
     2101 atggtcttgg aggatttcct caccttgccg gtatccttca ccagcaggag gaagccggcg
     2161 cccgcggcga cgcagatcag ccccggcacg aagaaggcgg cgcgccagcc ggcaaaatcc
     2221 accagcgcgc cggtgaacag cgccgccgcg gcgaggccca ggttgcccca caggccgttc
     2281 cagccgagcg tgcgccccat gttctgcgga ttggccacca gcatggcgat gccgaccggg
     2341 tggtagatcg ccgcgaaggc gccggtcagc gtaagcccgg cggcgatgtg ccagggcgac
     2401 tgggacaagc cggtcaggaa cagcgacacg ccgatgccga aatagaagat cgccagggtg
     2461 cggtagcggc tccagtggtc ggcgagccag ccggcgggga tggcaaacgc accgaaggcg
     2521 atgaagccgc ccagcgccag cggcagcagc tccgagtacg ggcggcccca ctcccggccc
     2581 agcgccacca ccgcggtggg gaacaccagc atcaccaggt gatcgagcag gtgccccagg
     2641 ttcaggaatg ggacgagcca gcggttctgg gtcatatctg ccgagcttac ctcgtaaaat
     2701 ccggggcatg gatagtgttt caggaatcgt gctggcgggc ggccaggggc ggcggatggg
     2761 cggtgtggac aaggggctgg tgccgctgcg cggcaagccg atggtcgcct gggtgctcga
     2821 gcgcctcgcg ccgcaggtgg acgggatcct ggtcaacgcc aaccagaatg cagaagccta
     2881 tgccgcgttt ggccatcccg tggtgccgga cgccatcggc ggcttcgccg gccccttggc
     2941 cggcctgcac gcgggcctgt ctgcggcatc gacgccgctg gtggtcaccg tcccctgcga
     3001 ctccccgttc ctcccgctcg acctggtggc gcggctgcgc aaggcgctcg gcgacaacga
     3061 cctcgcggtg gcgaggaccg gcgaccagcc gcacccggtg ttttccctgg tgcggcgcgc
     3121 ggtgcgcgcg cacctggaga gctacctcgc cggcggcggg cgcaagatcg acgcctggta
     3181 cgccacgctc aaggtggtcg aagtgccctt cgacgacgag gccggcgcgt tccgcaacat
     3241 caacacgcgc gaagaattga aggacgcatg agccgcatcg tccagatcgt cagcgccatc
     3301 gagggctacg acccggacgc gctgcacgta gagaaggcgc gcgcggcgat ccgcgcctgc
     3361 ctcgccccgg tgacgcaatc cgagcgcgtg ccggtgcgcg cggcgctcgg gcgcgtgctc
     3421 gccgaggaca tcgtgccggc gatcgacgtg ccgggccacg acaacacggc gatggacggc
     3481 tacgcggtgc gcttcgccga cctcggcgcc ggggaaaccg tgctcgagga gatcggtgcc
     3541 gcactggccg gaagatcctt cggcggcaag ctcgcggcgg gccagtgcgt gcgcgtcacc
     3601 accggcgcgg tgatgcccgc gggggccgac acggtggtgg tgcaggaagt ggtgaaggtg
     3661 aacggcgccc gggtgacgat tccgcctggc cagaaaaagt cgcagaacgt gcgctacgcg
     3721 ggcgaggacc tgcagaaagg caagccggtg ctcgcgcccg gcaaggcgct cggcaccgcg
     3781 gagctcggcc tcgtcgcctc gctcggcatc ggcgagatcg cggtgcggcg caggctgcgc
     3841 gtcgcattct tctccaccgg cgacgagctg gcctcggtcg gcggcacgct cagggaagga
     3901 gaagtctacg actccaaccg ctacacgctg cacgggatgc tcgcgcgcct cggggtggaa
     3961 atgcacgacc tcggcgtggt gcgcgacgat cccgcgctgc tggaggcggc gttccgcaag
     4021 gcggccgacg cggccgacgc gatcgtcacc accggcggcg tgtcggtggg cgaggccgat
     4081 ttcgtcaagc cgatgatggc caagctcggc gaggcgctgt tctggcgcat cgccatgcgt
     4141 ccggggcggc cgatggcctt cggcaggatc ggcaaggcgt tcctcttcgg cctgccgggc
     4201 aatccggtgg cggtgatggt gaccttcacg cagttcgtgc gcgaggctct gctccagctc
     4261 ggcggccgca cgggagagct gtcggtgccg ctgctcaagg tgccggcggc ggagccgctg
     4321 cgcaaggttg cgggccgcac cgagtaccag cgcggcatcc tgttccgcga cggcggcgac
     4381 tggaaggtga agaccaccgg ccagcagggc tcgggcgtgc tgcgctcgat gtcggaggcc
     4441 aactgcctca tcgtgctgga gcacgagcgc ggcacggtgg cggcgggcga gccggtgcag
     4501 gtgcagctgt tcgagggtct gctgtgaacc ccatccaccc ttcgctcgcg ccgcttgccg
     4561 tcatcgccaa gaaggatccg catctgctgc acgagcatct gcgcgctgcg ctcagcgacc
     4621 cggcctggct gcgtcccgag caccgcgtcg gcgatccgca cgcctacatg cgccacctgc
     4681 tgtacgtgga ccccgacggg gatttcgtca tcaccgcgat cacctggctg ccgggccagc
     4741 agagcccgat gcacggccac cacgtgtggt gcgccttcgg cgtcgccgag ggcgagctga
     4801 ccgaggagcg cttcgatgag cagggcgtat tgcagcggac ggtgcgctac gccgccggcg
     4861 acgtggcgcc gagctcgccg atccaccgcg tcgccaaccg cggccgcgcg ccgctggtgt
     4921 cgctgcacct gtacggcgtg agcgccgagc ggctcaccac cggcatcaac cgcatctacg
     4981 cctgatccca gagcatcgcc gcgccgcgga cgccgctcgc gtcgccgtgc gcagcgcgca
     5041 gcaccggggt ggagaatgcg tccgtgtagg catagcgcgc gatgagcggc ggcagctggt
     5101 cgtacagcga tgcgatgttg gacacgccgc cgccgagcac gatggcgtcc ggatccagca
     5161 gattgaccac ctgcgccagt gcgcgcgcca ggcggtcggc gtgcagcgcc gcgctcgccc
     5221 cgtcgcgccg cagcgcggtg cccgacacga actgctcgat gcagccggcg cggccgcagc
     5281 tgcagaccgg tccgggatac tcctcgggcc gcatccacgg cagcgggttg tggccccact
     5341 ctccggccag cccgtgatgc ccctccagcg cgcgcccgtc gaccaccagc ccgccgcccg
     5401 cgccggtgcc gagcaccgcg ccgaacacca cgcgcgcgcc gcgcgcagcg ccgtcgcgtg
     5461 cctccgacag tgcgaagcaa ttggcgtcgt tggcaaagcg cagctcgcgc cccagcgccg
     5521 cctcgaggtc gcgcttgagc ggccggccgt tcaccggcgt gttgtaggcg ttcgcgacca
     5581 gtcccgtgcg ccgcgacagc gtgcccggca ggccgatgcc gaccgtgcaa ggctcctgcg
     5641 cctcgcgctc cagttcggcg atctgcgtct gcagcagccg cagcgcgtcc tcataggcgc
     5701 cgcgcggcgt cggcaccctg cggcgcaagg cgaccttgcc ctgggcatcc aggaccgcgg
     5761 cctcgatctt gctgccgccg aggtccaccc cgaggcgcat cagaacgcct cgtcccagcc
     5821 gatcgacagc cgcgtcgccg aattcaccag ccccaccatg ctgtaggtct gcgggaaatt
     5881 cccccacaac tcgcgcgtcg ccggatcgac gtgctcggcg agcaggccgt gccggttgcg
     5941 gcagccgagc agatcctgga acagcgcgcg cgcctcgtcg cggcggccga cggcggcgag
     6001 cgcgtcgacg aaccagaaac tgcacaccag gaaggcgttc tccggcgcgc cgaagtcgtc
     6061 cggctcggtg tagcggaaca cgaaatcgcc cttcttcagc tcgcgctcca ccgccgccag
     6121 cgtggcgcgg tagcgtggat cgtcggcggg caggaaacgc agctcggcga gcagcagcag
     6181 gctcgcatcc agcccttcgc cgccgaaggt ggcggcgaag ctgttgcgcc tggcgctcca
     6241 cgcccgttcg cagatgccac catggatgcg ctgcgcgtgc gcctgccagt aggcggcgcg
     6301 tggcgcgagg ccgagccgtg ccgcgatctt ggccaggcgg tcgcaggccg cccagcacat
     6361 gacgctggag aaagtgtgca cgcggccggc gccgcgcagt tcccacagtc cggcgtcagg
     6421 ctcgttccac acggccaccg cgcgctcgcc gagcacctcg aggtggcgga acagcgcctc
     6481 gttgccgctg cgcatcaggc gctggtcgaa gaaggcgtgg gtggccgcca gcaccgccga
     6541 gccgtagacg tcattctgca cctgcaggta ggcctggttg cccacgcgca ccggccccat
     6601 gccgcggtag cccgccagcg tggcgatctc gcgctcctcg agctgcgccg caccgttgat
     6661 gcggtacacc ggctgcaggc gcgcgccctc cgcgcccgcc gccacgttga tgatgtagcc
     6721 caggtagcgc tccatggtgc gcgtggcatt gagccggttg agcgcgttga tggtgaaata
     6781 ggcgtcgcgc agccagcagt agcggtagtc ccagttgcgg ccgctcgcct tcgcctccgg
     6841 aatcgaggtg gtgaccgccg ccaccagcgc cccggtgtcg tccaccgccg agagcttgag
     6901 ggtgagcgcc gcgcggatga tctcgtcctg ccattcgaag gggatctgca ggtcgcgcac
     6961 ccactcgcgc cagtagtcgg cggtctcctc gtggaagcgg cgcgcggtct ccgccacccc
     7021 gccctgcagc gtctcgtccg gcccgaggat gaaagcgagc ggccgcagca gcacgaaggc
     7081 cagttcgtcc accagcgcgg tgatcgagca gtcggtggtc acccgcatga cgtagttctc
     7141 ggccacgtag cgcacgtggt tgctgccgta cgtgagcgcc gggcgcttgg cgccgtattg
     7201 cgatgccggg cgcagcagca cgcgcacgcg cgggctgccg gcgagcgggc gcaactcgcg
     7261 caccagggtc atcggcgaga agatgcgcgc gtggtggcgg aagcgcggca cgaaatccgt
     7321 gatttccacc gcgccgccgt gccggtcgta caggcgcgtc accaggatcg gcgtattggg
     7381 caggtacgcc tgctcgctgt gggagaaatc cgccagttcg aaggcgaaga agccgaagtc
     7441 gtcggcgccc ttgcgctcgc gcagcagcga gcaggccacc gggtcgccgt ccatgcgcgg
     7501 caggcaggcc cacacgacct cgcccttcgc gtcgatcagc gcggcgatgt tggcattgcc
     7561 gacgatcgcc agatccaggg tctggctcat gcgcgctgcg cggcccactg cgcaagctgc
     7621 tcgcgcaccg ccgccgcgtc ggcgaggcgg tggcgggccg ccgaggcgcc cggccccacc
     7681 ttcacggagt ggccgtccag gcggttcacc acgccgaagc cgaactcgtc ggtgacgtcg
     7741 tcgccgatga acaccggcgt gcgcccggcg aaaggcgctt cggccatgaa ttcggcgatc
     7801 gccctgccct tgtcgcggcc cgccggcttg agttcgacca ccatcttgcc ctccagtacc
     7861 tcgtagtccc cgcccagctc cagcgccgcc gcgcgcagcg ccgcctgcgc ggcctgcgcg
     7921 gctgccggcg cctgccggta atgcagcgcg agattgtgac ccttgtcctc gaacaccaac
     7981 ccggcatgcc gcgcggcgaa cgcgcccagt cgtgccgcgg cacggcgcac cggctcggcg
     8041 gcgccctggt gccggtgcag gcgcccggcc gcgtcgcgcc gctctgcgcc gtgctgcccc
     8101 gccacgggca gccgcagcgg cgcgaacagg gcatcgatgt cgtcgatcgc gcggccgctg
     8161 atcagcgcca gcgcgccgcg cgtggcgagc cgcagcgcct cgagcagctg gtgcacgggc
     8221 gcggccacgc gcacggcgtc cggacgcgcg gcgtgctcca gcagggtgcc gtcgacgtcc
     8281 aggaacagcg cccagtcggt gaccggcggc agagcgcgct ggccgtgcgc gctcatccgg
     8341 tcccggcctc gcgccgcgcg gcgcgcgcgc gcgacagcac gcgccgccgg tggcgcatgc
     8401 gcgcggcgtc gagcagcatg cgtccggccc agcggtagac gttgaaatcc tgcaccagcg
     8461 tgcgcatgct gcgcatgcgc gcgcgctgct cggccggcgg catggtgagg gcgaggtgca
     8521 tggccgcggc gctctgctcg atgtcgtagg ggttcacggt gagcgcctcc accagctcgc
     8581 gcgccgcgcc cgtgaactgc gacaggatca gcacgccctg ctggtcgtcg cgcgccgcga
     8641 cgaactcctt ggccaccagg ttcatgccgt cgtgcaggct ggacacgcag cagaaatcgg
     8701 cggcgcggta cagctcgtac acctgcgccg cgtcgtggtg ctcgatgcgc agcacgatcg
     8761 gctcgtagcc ggccgaaccg aagcgccggt tgacgcgcgc ggcgagctcg cgcacgcgcg
     8821 cctccaggtt gcggtactcg tcgatcgagg agcgcgaggg cgccgccgcc tgcacgaagg
     8881 cgaacttgcc gacccagcgc ggctcgagtt ccagcagccg ctccaccgcc atgaaccgct
     8941 cgaggatgcc cttggtgtag tccaggcggt ccacgcccac gccgagcgcc acctccgcgg
     9001 ccaggccgag gcgcgcgcgc aaggcgcggc gcgcctctgc aatgggggcc tgggcggcga
     9061 gcgccgtggg cggccactcg atcgagatcg ggtagcggcg cacttccgtc ggcgcgccgc
     9121 cgtaggagat ggtgaaggtc tcgcggtcca cgcgcgtctc gagaaagcgg tccacggtgt
     9181 cgaagaagtt gttgcagtgg aactgcgtgt ggaagccgac gatcgaggag ccgagcaggc
     9241 cctccagcac ttcctcgcgc cagggcagga tgccgaaggc ctccgggttg ggccagggga
     9301 tgtgccagaa ggcgatgatg gtggcgcgcg gcaggcgctc gcgcaccatg cgcggcagca
     9361 gcgcgaaatg gtagtcctgc accaggatga tcgggtcctc ggtgcgcgct tcgtccacca
     9421 ccaccttggc gaagcgctgg ttgaccgcct tgtagtgctc ccagtcgtcg gcgcggaaca
     9481 ccggccgcgt gtgcgcgatg tggcacaggg gccacaggcc ctcgttggcg aagccgtagt
     9541 agtagccctg ctcctccgcc ttcgacagcc agacgcggcg gatgcggtag gcgggcttgt
     9601 cgggcggcac catcacgtag tcgccctggt ccaccgtgtc ccggtcggcg gagcccgcgc
     9661 cgtgcgcgat ccaggtgccc gagcaggcgc gcattaccgg ctcgagcgcc gtcaccaggc
     9721 cgctcgccgg ccgctggatc tcgatcacgt tgtccttgcg gcgcacgtgg atgtagggtt
     9781 cgcggttgga cacgatcagc acctcgtcgc ccttgaggtc ctcgcgcagc accccgcgca
     9841 gggcctccgg cccccaggtc atctgcgtct cgtcgcggac gtggcgctcg gcctccaggt
     9901 cgcgcaccag ggcctgcagg tcgcgcgcga tcggccgcaa ttccggcgcc gtcaccctga
     9961 gcccctcgcg cgtgccgagc agcgcctcgc cggagatgat gcgtttcatg ccggccaccc
    10021 agccgcgcca gctgatctcg gcgatcacca ccgtgatcag cgccaccacg gcggcgatgg
    10081 cggcgaacag ccagaccacg tacttcttcg tgtcgtcgct gcgccgcgtg acgaagctca
    10141 tgtcgtgcac gatcaccagg tcgccgagca cttcacggtc cgcgaggatc gggatcgccg
    10201 ccacgtgcag cggaccctgc gtgtgctgca ggcgcccggt gtacgcctcg cgccctgtcg
    10261 aagcgcacaa ctggctgccc gggaaggtgc cggtggcgaa gggcagcttg ccgtcgcgct
    10321 cgcagaagcc gagggcgaag atgcgctcgt cctggatgat gtgctcgaag tagctcatca
    10381 ccttgggccg cgcgtccggc tcgcgcacca gcgccgcgag ccgttcctgc gccgcgttgg
    10441 cgatggtggt ggcccgcatc tccaggtctc gctcgaacca ctgcagtgtc aggcggtcca
    10501 ccagcggcac cacggcgtag gcgatcgcgg cgagcgcaag gccgagaggg acgatgaacc
    10561 gcagggacaa ccgcatgggg acccccgtcc ggcgagaacc gccggctgat gggaacagtc
    10621 ggatcttaca gatctgtcag tgcgctgtaa accattttaa atcatgcaat tagattgcca
    10681 tcgtgcgcgc aagatcgagc agggcggcat cggccgggcg tgtgcgcccg ccgatctcgg
    10741 cgagcgcagt ggcgctgatc tcgccacgct gctggcccat cagcacgccg ctgcggccgg
    10801 cggcgaggct ctccacggcc gccgcgccca gccgcgtggc gaggacgcgg tcggccgcgg
    10861 tcggcacccc gccgcgcacc acatgcccca agcgcgtcag gcgcaactcg aagccgatgg
    10921 tgtccttctc ctcgccgtag tgcttgagca gcgcctgcac cccgtgcctg gcgccctcag
    10981 ccatcaccac cagcgcgtgc gtcttgccgc ggcggtacgc ggcgcgcagc cgctcggcga
    11041 tctccgccgg ctcgacctcg cgctcgggga tggcgatcac ctcggcgccg cctgcgatgc
    11101 ccgccagcag cgcgatgtag ccgcaatcgc ggcccatggt ttcgaccacg aaggcgcgct
    11161 ggtgcgacga ggcagtggtg cgcaggcggt cgatcgcctc cagcgtcacg ttgatggcgg
    11221 tgtcggcgcc gatgctgatg tcggtgccgt acaggtcgtt gtcgatggtg gaaggcacgc
    11281 cgacggcggc aaagccttcg cgcgccagcg cggccgtgcc ggtctgcgag ccattgccgc
    11341 cgatcaccac cagggcgtcg atgcggcgcg cggcgaggtt gcgcagcgcc ctttcgcgcc
    11401 ccggcgtctc gatgaactcc gggcagcgcg cgctgccgag gaaggtgccg ccgcgctgca
    11461 cgatgccgcc gacatcgcgc gcggcgagcg gctcgagcgt gtcggcgagc aggccggcgt
    11521 agccgttgcg cacgccgtag gtctcccagc cgcgggccag cgccgcgcgc gtgactgcgc
    11581 gcagcgcggc gttcatgccg ggtgcgtcgc cgccgctggt gaggaccgcg aggcgcttca
    11641 tgtcaggact tgcgcccctc ctccaaccgc acgatctcgg ccggcggccg ctcctcgagt
    11701 tcagcggact cgatgcgctg gaactgctgc gcgatcttgc cgccggtcac ctggatgcgc
    11761 tcggcgtctt cgtgcgccag gcgaatatgg tcggcgagct tcttcaggcg gtcgtcgaac
    11821 ctgccgaatt cgaccgccag gcgcccgagc gcttccttga taacgtggat ctgcttgcgc
    11881 gtctcgacgt ccttcagcac tgcgcgcgcg gtgttcagca ccgccatcag cgtggtcggc
    11941 gacacgatcc acacgcgctt accgatcgcg tagtccacca cctcgggatg gtaggcgtga
    12001 atctccgcga acaccgcttc tgccggcacg aacatcaccg cgccgtccga ggtctcgttg
    12061 tcgatgatgt atttcgacgc aatcgcgtcg acgtgcttcc tcacgtccag ccggaacgcc
    12121 ttctgcgcaa tcgcccggtc ggcctcgccg gccttcgcat cgagcatcag cttgtagttc
    12181 tcgaggggaa acttcgagtc cacgcacacc aacccggtag gcgcgggcag ccgcagcacg
    12241 cagtccacgc gcgcgccatt ggacagcgtg tactgcattt cgaaggcctg gttcggcagc
    12301 acgttgcgca ccagcgcctc cagctgcact tcgccgaagg cgccgcgtga acgcttgtcg
    12361 ccgagcagtt cctgcaggct cacgacgctg ccggtcagcg tctcgatctt cttttgtgcc
    12421 tcgtcgatgg tcgccagacg ctgcatcacg ttgacgaagg tgtcgttggt cttcctgaag
    12481 ccctcgtcga gccgctcatt gacgcgcagc gaaatctggt ccaggcgcgc gtcgagcttg
    12541 gccgccaccg cctcccgcag ctcgccaagc tgggcgccca gccggtcgcc ctgcttcacc
    12601 aggccgtcgt gcaggtcggt gagcatctcg cggtgcttgc gctccgcgcc gccctgcagc
    12661 cgcagggcca gccagacgac cgcggccagc gcgcccagcg ccaccagcag cgcgatgatg
    12721 gacagcgatt cgagcatgcg cgcattctag cgcgcggcgc tgtgctagcc gcgcaggcgc
    12781 acgctcacgc gggtgccgct gcccggcgcg gactcgacgc gcaactgccc gccgatggcc
    12841 gcggcgcgtt cccgcatcag cgtgagcccc agggcatcgc tcgggcgcgc ggcctgggtg
    12901 tcgaagcccg cgccgtcgtc gcggatcgac agcagcacta cgccgccgcc gaccgatagc
    12961 tccaccgccg cgttgcgcgc gccggaatgc cgcgccacgt tgtccagcgc gtcgcgcgcg
    13021 atctggaaca gcgccgtctc caggcgcggc tcgatctcgg cgccatcctc ggcgccgatc
    13081 acggcgagcg caatgccggt gcggcgttcg aagcgcaccg cgtgggtgcg cagcgcagcc
    13141 agcagtccgt agtcctcgag cgccgccggg cgcaactgcg caaccagctc gcgcaccgcc
    13201 gcgaggggcg ccttcagccg cgccaggccg gaatccgcca gcgccgccgc cagggggcgc
    13261 gccaccatgt cgcgcaattc cgtccccacg tgtcgccgct cggcttcctg cgcgtcgagc
    13321 agccgccgcg acaagccgcg catgcgcgct tcggactcgc gggtgcgctc gtcgccacgc
    13381 gtgcggaata tgtactcgcc gagcagcgcg ccgagggatg tcgccatctc cagcagctcg
    13441 tgctccggct cgcccggcgc gctgccgcgc agctcgatca cgccgagcag caattccccc
    13501 gcggtgaccg gcatggcgaa ggcatgtccg tccgcggcgc cccacacggc acggcgctcg
    13561 cgccaggcgc gcacgggcag cgccgcgagc tggtccgcgc cgagcacgct ggacgccgcc
    13621 tcggcgcccg ccggatagac cgcaacggcg gcaagccgtg cgcccgcctc gtcggcgcgc
    13681 cagtaccggg cggtctccca gtcgagcttg tcgcacagga tgcgcaggct gccgagcacc
    13741 gccggttcca cgcccggggc ccgcgccagc atcagcccga tctcgtgcag ggcggactgg
    13801 atc
//
LOCUS       DASRIE010000064        10250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3730654, whole genome shotgun sequence.
ACCESSION   DASRIE010000064 DASRIE010000000
VERSION     DASRIE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730654"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFB53_02870"
     CDS             complement(<1..378)
                     /locus_tag="VFB53_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZN85706.1"
                     /translation="MLDKRAQLLLKTLVERYIAEGQPVGSRTLSRHSGLDLSPATIRN
                     VMSDLEELGLISSPHTSAGRVPTPAGYRFFVDTLLVMKPLGSGELHQLEGQLHPDSPQ
                     RAIQSASQLLSQLTQFAGVVMTPR"
     gene            414..1304
                     /locus_tag="VFB53_02875"
     CDS             414..1304
                     /locus_tag="VFB53_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZN85707.1"
                     /translation="MPLPAFRRVALIGKHTAEIAESLRSLRGLLRERGCEVRVERDTA
                     ARIGEKEGAASYEEIGAFADLAVVLGGDGTMLAAARNLARYKVPLAGINQGRVGFMTD
                     IALKDLRSSVGAILDGRHSIEERALLDAEVRRGGKSLLRTLALNEAVVGKGSQGRLIE
                     VEVHIDGEYVYALRADGLIVATPTGSTAYALSAQGPILQPGVAAFALVPLNPHVLSAR
                     PVSVSDRSTIEIALLRAVDARAHFDGFALTDMAEGDRLVLRRSADAMRFVHPPGYSYF
                     AMLREKLSWSGSAEKAPEPD"
     gene            1336..2994
                     /gene="recN"
                     /locus_tag="VFB53_02880"
     CDS             1336..2994
                     /gene="recN"
                     /locus_tag="VFB53_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZN85708.1"
                     /translation="MLRVLSVRDYVIAERVELEPGAAFGVLTGETGAGKSILVDALEL
                     LVGGRAESALVREGAERAELSAEFEVGAGSALAAWIAAAGLEGDAGSLILRRSMDAAG
                     RSRCFVNGHAATLAQLREAGEFLVDIHGQHAHQSLLRPAAQRELLDVHGGAPELARDC
                     AQAYRDWKRLQALAAEAEQQYAQREAERAELQERVSVLDRLAPLAGEWERLAAEHRRL
                     AHGSSLLAGAQSSLETLSEAEGASLPQLSAVTARLSELSAHDAQLREIVEMLESAEAQ
                     AGEAVRALRNYASRVELDPEALREAEARIEALHAAGRRFRVRPEELVALAETQRARLK
                     DLDLAASPEALQREVAAAEARTLAAAKKLSAKRKSTAQALSKAVTEGMQQLAMKGGRF
                     SVALRALEAPTAAGMEDVEFEVSSHPSLPLRPLAKVASGGELSRVSLALQLVTAKASP
                     VATLVFDEVDAGIGGAVAETVGRALKKLGKDRQVLCVTHLPQVAAQGDEQWTVTRSAT
                     RGKVGVGAARLDRAGRIEELARMLGGAQITDTTRRHAAEMLGAG"
     gene            complement(2991..3428)
                     /gene="fur"
                     /locus_tag="VFB53_02885"
     CDS             complement(2991..3428)
                     /gene="fur"
                     /locus_tag="VFB53_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZN85709.1"
                     /translation="MDSAQSLKDSGLKATLPRRKVLELFEQSKVRHLSAEDVYRSLIA
                     DGIDIGLATVYRVLTQFEQAGLLSRQHFEAGKAVFELNQGGHHDHLVCLQCGRVEEFY
                     DAEIEKRQSEVARKGGFALRGHSLALYGDCTKQDCPNRKSLQT"
     gene            3448..3798
                     /locus_tag="VFB53_02890"
     CDS             3448..3798
                     /locus_tag="VFB53_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZN85710.1"
                     /translation="MARAPFTLLPLACACALAGCGILEPYRIDIQQGNFVSQDMVAQL
                     KPGMTKEQVRFVLGTPLVTDIFHAERWDYVYTLERRGAPRQKRHLAVFFQDGKLARLD
                     GDVAAAEPAKAGER"
     gene            3795..4568
                     /gene="dapB"
                     /locus_tag="VFB53_02895"
     CDS             3795..4568
                     /gene="dapB"
                     /locus_tag="VFB53_02895"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZN85711.1"
                     /translation="MKLAIAGAGGRMGQALIDAVLADQGLDLVAAFDAAGSPALGRAA
                     GKVKVQADLGGLSAADVLVDFTRPAGTLLHLEACLKFGKAMVIGTTGFSEAQQQRIAA
                     GAKRIPIVMSPNFAIGVNVVFRLAQTAAQALGDDYDVEIVEAHHRHKVDAPSGTALKL
                     GELVALALGRKLGEVATHGRAGDTGERPAKAIGFHAIRGGDIVGEHTVIFAGAGERVE
                     VAVRSQSRLTYAAGALRAARWLEGRGPGLHDMFDVLGLK"
     gene            4652..5791
                     /gene="carA"
                     /locus_tag="VFB53_02900"
     CDS             4652..5791
                     /gene="carA"
                     /locus_tag="VFB53_02900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZN85712.1"
                     /translation="MPVLDSAVLVLADGTVFRGRSVGAAGLSAGEVVFNTAMTGYQEI
                     LTDPSYCGQIVTLTCPHIGNTGANAEDDEAGKVYAAGLVVRDLPASHSSWRAQQSLPQ
                     LLADRKVVAIADIDTRKLTRLLREKGAQNGCLMAGTVDLGKALAAARAFPGLAGMDLA
                     KVVTTAKPYEWRDGKWSLGEGYRRIEGAPYHVVAYDYGIKRNILRLLAERGAHVTVVP
                     AQTPAREALRLQPDGVFLSNGPGDPEPCDYAIEAIGEILDATRVPVFGICLGHQLMGL
                     ASGAKTLKMKFGHHGANHPVQDLETGQVVITSQNHGFAVDPKSLPSTLKPTHVSLFDG
                     SLQGLARTDRPAFCFQGHPEASPGPHDIGYLFDRFAALMKHAKAL"
     gene            5775..9026
                     /gene="carB"
                     /locus_tag="VFB53_02905"
     CDS             5775..9026
                     /gene="carB"
                     /locus_tag="VFB53_02905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZN85713.1"
                     /translation="MPKRSDLHSILIIGAGPIVIGQACEFDYSGAQACKALRDEGYRV
                     VLVNSNPATIMTDPEMADATYIEPITWQTVARIIEKERPDALLPTMGGQTALNCALDL
                     AREGVLEKFGVEMIGASREAIDKAEDREKFKTAMTRIGLASAKSSLAHSLEEALQVQA
                     SLGFPVVIRPSFTLGGTGGGIAYNREEFVAICERGLEASPTNELLIEESVIGWKEFEM
                     EVVCDRKDNCIIVCSIENVDAMGVHTGDSITVAPAQTLTDKEFQIMRNASIAVLREIG
                     VDTGGSNVQFAINPQDGRMLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEITGGATPASFEPSIDYVVTKVPRFAFEKFPQANDRLTTQMKSVGEVMAIGRSFQ
                     ESLQKALRGLEVGVDGLNQKTRDRETLERELGEPGPERIWYVGDAFENGFSLEEVHRL
                     THIDPWFLAQIKEIVDLEMRLDDQQLAEVDAGTLRMLKRKGFSDRRLAYLFNTGESAV
                     REKRLKLGIRPVFKRVDTCAAEFATRTAYLYSTYEEECEAEPSARRKVMVLGGGPNRI
                     GQGIEFDYCCVHAALALREEGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     DKEKPWGVIVQYGGQTPLKLARDLAAAGVPIIGTSADSIDVAEDRERFQQLLHKLKLR
                     QPPNRTARSVAEAVKHAEAIGYPVVVRPSYVLGGRAMEIVHHQADLERYMKSAVKVSN
                     DSPVLLDRYLSDAIEVDVDCIADGTEVLIGGVMEHVEQAGVHSGDSACCLPPHSLSAE
                     LVQELRRQTEVMARALQVCGLMNVQFAIQQSEQGSTVYVLEVNPRASRTVPFVSKATG
                     RPLAKIAALCMAGKTLAAQKVQGDITPPYYSVKEAVFPFAKFPGVDTILGPEMKSTGE
                     VMGVGATFGEAFVKSQLAASIRLPTSGRAFISVRDGDKRAAVGVARDLAALGFTVLAT
                     RGTAAVLAEHGLAVTPVNKVAEGRPHIVDMIKNGEVTFIANTVEDTRTAISDSRAIRV
                     TALAQRVTYYTTIAGAKAACAGMQHMTQLEPYRLQDLHAKLGLPTPVRAIGSH"
     gene            9026..9499
                     /gene="greA"
                     /locus_tag="VFB53_02910"
     CDS             9026..9499
                     /gene="greA"
                     /locus_tag="VFB53_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZN85714.1"
                     /translation="MAKTPMTLRGAELLRVELHRLKTVERPANSQAIAEARAHGDLSE
                     NAEYDAARNQQGFIEGRISELEAKLGNAQIIDPKLLDADGRCVFGATVDLEEKGGKAI
                     TYQIVGEDEADIKAQRISISSPIARALIGKEAGDEVDVHTPGGVKRYEILDVRYE"
     gene            complement(9503..9922)
                     /locus_tag="VFB53_02915"
     CDS             complement(9503..9922)
                     /locus_tag="VFB53_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HZN85715.1"
                     /translation="MDHITGAQKKALKARAHALHPILQLGEKGLTDAVVAEIERALGA
                     HELIKVRAAPLNRDEREVALASICERTGAHAVQHIGKILVVYRQKPPAEPKAAPRRRT
                     QAASGWDERKDARRKPARRRKSAGPLRPQRRRAGERR"
     gene            9947..>10250
                     /locus_tag="VFB53_02920"
     CDS             9947..>10250
                     /locus_tag="VFB53_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZN85716.1"
                     /translation="MAKSNKAWLRRHVTDSYVRRAREAGYRSRAAYKLLEFVAKDRLL
                     KPGMRVLDLGAAPGGWSQVAAEIVQPNGKVVAVDLLELRPIQGVTICKGDFREADLD"
BASE COUNT         1600 a         3504 c         3697 g         1449 t
ORIGIN      
        1 gcgaggggtc atcacgacgc cggcgaactg ggtgagctgc gacagcagct gggaggccga
       61 ctggatggcg cgctgcgggc tgtccgggtg cagctgcccc tcgagctggt gcagctcgcc
      121 cgagccgagc ggcttcatca ccagcagcgt gtcgacgaag aagcggtagc ccgccggggt
      181 cggcacccgc ccggcggagg tgtgcgggct ggagatcagg ccgagctcct ccaggtccga
      241 catcacattg cggatggtgg cgggggacag gtccagcccg gaatggcgcg acagggtgcg
      301 cgagccgacc ggctggccct cggcgatata gcgctcgacg agcgtcttca gcaatagctg
      361 cgcacgtttg tcgagcatgc ggtgcatttt acacatgcgg ttagaatgcc gcgatgccct
      421 tgcccgcgtt ccgccgcgtc gcgctgatcg gcaagcacac ggcggaaatc gccgagtcgc
      481 tgcgctcgct gcgcggcctg ctgcgcgagc gcggctgcga ggtgcgcgtg gagcgcgaca
      541 ccgcggcgcg catcggcgaa aaggagggcg ccgccagcta cgaggagatc ggcgccttcg
      601 ccgacctggc cgtcgtgctg ggcggcgacg gcaccatgct ggccgcggcg cgcaacctgg
      661 cgcgctacaa ggtgccgctc gccggcatca accagggccg cgtgggcttc atgaccgaca
      721 tcgcgctgaa ggacctgcgc agctcggtgg gcgcgatcct cgacggccgg cacagcatcg
      781 aggagcgcgc gctgctggac gctgaggtgc ggcgcggcgg caagtccctg ctgcgcaccc
      841 tggcgctcaa cgaggccgtg gtgggcaagg gctcgcaggg gcggctgatc gaggtcgagg
      901 tgcacatcga cggcgaatac gtctatgcat tgcgcgccga cggcctgatc gtcgccacgc
      961 ccaccggctc caccgcctat gcgctgtcgg cgcagggccc gatcctgcag cccggcgtgg
     1021 cggccttcgc gctggtgccg ctcaacccgc acgtgctctc ggcgcgcccg gtgagcgtca
     1081 gcgaccgcag caccatcgag atcgcgctgc tgcgcgcggt ggacgcgcgc gcgcacttcg
     1141 acggcttcgc cctcaccgac atggccgagg gcgaccgcct ggtgctcagg cgctcggcgg
     1201 acgcgatgcg cttcgtgcat ccgccgggct acagctattt cgccatgctg cgcgagaagc
     1261 tgagctggag cggctcggcc gagaaggccc ccgagccgga ctgagcggcc aacctgcgcg
     1321 tccgctgagc agcccatgct acgagtccta tcggtgcgcg actacgtcat cgccgagcgc
     1381 gtcgagctgg agccgggcgc cgcgttcggc gtgctcaccg gcgagaccgg cgccggcaag
     1441 tcgatcctgg tggacgcgct ggagctgctg gtcggcgggc gcgccgagtc ggcgctggtg
     1501 cgcgagggcg ccgagcgcgc cgagctctcg gcggagttcg aggtcggcgc cggctcggcg
     1561 ctcgccgcct ggatcgccgc ggccggcctg gagggcgatg ccggcagcct gatcctgcgc
     1621 cggagcatgg acgccgcggg ccgctcgcgc tgcttcgtca acgggcatgc ggcgacgctg
     1681 gcgcagctgc gcgaggcggg cgaattcctg gtggacatcc acggccagca cgcgcaccag
     1741 tcgctgctgc ggccggcggc gcagcgcgaa ctgctcgacg tccatggcgg tgcgccggaa
     1801 ctggcgcgcg actgcgccca ggcctaccgc gactggaagc gcctgcaggc gctcgccgcc
     1861 gaggcagagc agcagtacgc gcaacgcgaa gctgagcgcg cggaactcca ggaacgggtg
     1921 tcggtgctgg atcgccttgc gcccttggcg ggagagtggg agcggctggc ggccgagcac
     1981 cggcgcctcg cccacggctc gagcctcctc gccggcgccc agtcttcgct cgagaccctg
     2041 tcggaggccg aaggcgccag cctgccgcaa ctctcggcgg tcaccgcgcg cctgagcgag
     2101 ttgtccgcgc acgacgcgca gctgagggag atcgtcgaga tgctggagtc cgccgaggcg
     2161 caggcgggcg aggcggtgcg cgcgctgcgc aactacgcgt cgagggtgga gctcgacccg
     2221 gaagcgctgc gcgaggccga agcgaggatc gaggcgctgc acgccgccgg acgccgtttt
     2281 cgcgtgcggc cggaggaact cgtcgccctg gccgaaacgc agcgcgcccg gctgaaggat
     2341 ctcgacctgg cggcgtcgcc cgaggcgctg cagcgggaag tggcggccgc cgaggcgcgc
     2401 accctggcag cggcgaaaaa gctgtccgcg aagaggaaga gcaccgccca ggccctgtcc
     2461 aaggcggtca ccgagggcat gcagcagctg gccatgaagg gcgggcgctt ctcggtggcg
     2521 ctgcgcgcgc tcgaggcgcc gaccgcggcc ggcatggagg acgtcgaatt cgaggtgtcc
     2581 tcgcacccga gcctgcccct gcggcccctc gccaaggtgg cctcgggcgg cgagctctcg
     2641 cgcgtcagcc tggcgctgca gctggtcacc gccaaggcct cgccggtggc cacgctggtg
     2701 ttcgacgagg tcgacgccgg catcgggggc gccgtggcgg aaaccgtggg ccgggcgctg
     2761 aagaaactgg gcaaggaccg ccaggtgctg tgcgtgacgc atctgccgca ggtggcggcg
     2821 cagggcgacg agcaatggac ggtgacgcga tcggccacgc gcggcaaggt cggcgtcggt
     2881 gcggcgcgcc tggaccgggc aggacgcatc gaggaactgg cgcgcatgct gggcggcgcg
     2941 cagatcaccg acaccacgcg ccgccacgcg gcggaaatgc tcggcgccgg ctaggtctgc
     3001 agggacttgc ggttcgggca gtcctgcttg gtgcagtcgc cgtacagggc gagcgaatgg
     3061 ccgcgcagcg cgaagccgcc cttgcgcgcc acctcgctct ggcgcttctc gatctcggcg
     3121 tcgtagaact cctcgacgcg cccgcactgc aggcacacca ggtgatcgtg gtgcccgccc
     3181 tggttgagct cgaacaccgc cttgccggcc tcgaagtgct ggcgcgacag gaggccggcc
     3241 tgctcgaact gcgtcagcac gcggtacacg gtggcgaggc cgatgtcgat gccgtcggcg
     3301 atcaggctgc ggtagacgtc ctccgccgac aggtggcgca ccttgctctg ctcgaacagc
     3361 tccagcacct tgcgccgggg cagggtggcc ttgaggccgg aatccttcag gctctgcgcg
     3421 gagtccatcc gctatcatta taggctcatg gcccgcgcgc ctttcacgct gctcccgctc
     3481 gcctgcgcct gcgcgctggc gggctgcggc atcctggagc cctaccgcat cgacatccag
     3541 cagggcaact tcgtgtcgca ggacatggtg gcgcagctga agcccggcat gaccaaggag
     3601 caggtgcgct tcgtgctcgg cacgcccctg gtcaccgaca tcttccacgc cgagcgctgg
     3661 gactacgtct atacgctgga gcggcgcggc gcgccgcgcc agaagcgcca cctcgcggtg
     3721 ttcttccagg acggcaagct ggcgcgcctg gacggcgacg tggcggcggc cgagccggcc
     3781 aaggcggggg agcggtgaaa ctcgccatcg ccggcgcggg cgggcgcatg gggcaggcgc
     3841 tcatcgacgc ggtgctcgcc gaccaggggc tcgacctggt ggcggcgttc gacgccgccg
     3901 gcagcccggc gctcgggcgc gcggcaggga aagtgaaggt gcaggccgat ctcggcggcc
     3961 tgtcggcggc cgacgttctc gtcgatttca cccgccccgc cggcaccctc ctgcatctcg
     4021 aggcctgcct gaagttcggc aaggccatgg tgatcggcac cacgggattc agcgaggcgc
     4081 agcagcagcg cattgccgcc ggcgcgaagc gcatcccgat cgtcatgtcg cccaatttcg
     4141 ccatcggcgt gaacgtggtg ttccgcctgg cgcagaccgc ggcgcaggcg ctgggcgacg
     4201 attacgacgt cgagatcgtc gaggcgcacc atcgccacaa ggtggatgcg ccctccggca
     4261 ccgcgctgaa gctgggagaa ctggtggcgc tcgccctggg gcgaaagctc ggcgaggtcg
     4321 cgacccacgg ccgcgccggg gacaccggcg agcgcccggc caaggcgatc ggtttccacg
     4381 ccatccgcgg cggcgacatc gtcggcgagc acacggtgat cttcgccggc gcgggcgagc
     4441 gcgtggaggt ggcggtgcgc tcgcaaagcc gcctgaccta cgcggcgggc gcattgcgcg
     4501 ccgcgcgctg gctcgagggc cgcgggccgg ggctgcacga catgttcgac gtgcttggcc
     4561 tgaagtagcc ggcggggtat aattcgccgc tcagacgagc gggaggggcg caagcctctt
     4621 ccgctttttc acatctaccc gggagttagc cgtgcctgtg ctcgattccg ccgttctcgt
     4681 cctcgccgac gggacggtgt ttcgggggcg cagcgtgggc gccgcggggc tgtcggccgg
     4741 cgaagtggtg ttcaacaccg ccatgaccgg ctaccaggaa atcctcaccg acccgtccta
     4801 ctgcgggcag atcgtgacgc tgacctgccc gcacatcggc aacaccgggg ccaacgccga
     4861 ggacgacgag gccggcaagg tctacgccgc gggactcgtg gtgcgcgacc tgccggcgtc
     4921 gcactccagc tggcgcgcgc agcaaagcct cccgcagttg ctggcggacc gcaaggtggt
     4981 ggcgatcgcc gacatcgaca cgcgcaagct cacgcgcctg ctgcgcgaaa agggcgcgca
     5041 gaacggctgc ctgatggccg ggaccgtgga cctgggcaag gcgctggcgg cggcgcgcgc
     5101 cttccccggc ctcgccggca tggatctcgc caaggtggtg accaccgcga agccctacga
     5161 gtggcgcgac ggcaagtggt cgctgggcga gggctatcgg cgcatcgagg gcgcgccgta
     5221 ccacgtggtg gcctacgact acggcatcaa gcgcaacatc cttcggctgc tcgccgagcg
     5281 cggcgcgcac gtcaccgtgg tgccggcgca gacgcccgcg cgcgaggcat tgcgcctgca
     5341 gccggacggg gtgttcctgt ccaacggtcc cggcgacccg gagccctgcg actacgccat
     5401 cgaggcgatc ggcgagatcc tcgacgccac gcgcgtgccg gtgttcggca tctgcctcgg
     5461 ccaccagctg atgggcctcg cctccggggc aaagacgctg aagatgaagt tcggccacca
     5521 cggcgccaac cacccggtgc aggatctgga gaccgggcag gtagtgatta ccagccagaa
     5581 ccacggcttc gcggtggacc cgaagtccct gccgtccacc ctcaagccca cccacgtgtc
     5641 gctgttcgac ggctctttgc aggggctggc gcgcaccgac cggccggcgt tctgcttcca
     5701 ggggcacccc gaggcgagcc ccgggccgca cgacatcggg tacctgttcg accgcttcgc
     5761 ggcgctgatg aaacatgcca aagcgctctg acctgcattc gatcctgatc atcggcgccg
     5821 gcccgatcgt catcggccag gcctgcgagt tcgactattc cggcgcgcag gcctgcaagg
     5881 cgctgcgcga cgagggctac cgcgtggtgc tggtgaattc caacccggcc accatcatga
     5941 ccgacccgga gatggccgac gccacctaca tcgagccgat cacctggcag acggtggcgc
     6001 gcatcatcga gaaggagcgg cccgacgcgc tgctgcccac catgggcggg cagacggcgc
     6061 tcaactgcgc gctcgacctg gcccgcgagg gggtgctgga gaaattcggc gtcgagatga
     6121 tcggcgcctc gcgcgaggcg atcgacaagg ccgaggaccg cgagaagttc aagacggcga
     6181 tgacccgcat cggcctcgcc tcggcgaagt cgtcgctggc gcactcgctg gaggaggcgc
     6241 tgcaggtgca ggcgagcctc ggcttcccgg tggtgatccg gccctccttc accctcggcg
     6301 gcacgggcgg cggcatcgcc tacaaccgcg aggagttcgt cgccatctgc gagcgcggcc
     6361 tggaggcctc gcccaccaac gagctgctga tcgaggaatc ggtgatcggc tggaaagagt
     6421 tcgagatgga ggtggtgtgc gaccgcaagg acaactgcat catcgtgtgc tcgatcgaga
     6481 acgtcgacgc gatgggcgtg cacacgggcg actcgatcac cgtggcgccg gcgcagaccc
     6541 tgaccgacaa ggaattccag atcatgcgca acgcctcgat cgcggtgctg cgcgagatcg
     6601 gcgtggacac cggcggctcc aacgtgcaat tcgccatcaa tccgcaggac gggcgcatgc
     6661 tggtgatcga gatgaatccg cgcgtcagcc gctcgtccgc gcttgcctcc aaggccaccg
     6721 gctttcccat cgccaaggtg gccgccaagc ttgccgtcgg ctacacgctg gacgagctga
     6781 ggaacgagat caccggcggc gccacgccgg cttccttcga gccgagcatc gactacgtgg
     6841 tcaccaaggt gccgcgcttc gccttcgaga agttcccgca ggccaacgac cgcctcacca
     6901 cgcagatgaa gtcggtgggc gaggtgatgg ccatcggccg cagcttccag gagtcgctgc
     6961 agaaggccct gcgcgggctc gaagtgggcg tcgacggcct caaccagaag acgcgcgacc
     7021 gcgagacgct ggagcgcgag ctgggcgagc cgggcccgga gcgcatctgg tacgtgggcg
     7081 acgccttcga gaacggcttc tcgctggaag aggtgcatcg cctcacgcac atcgatccct
     7141 ggttcctcgc ccagatcaag gaaatcgtgg acctggagat gcgcctggac gaccagcagc
     7201 tcgccgaggt ggacgccggc accctgcgca tgctcaagcg caagggcttt tccgaccggc
     7261 gcctcgccta cctgttcaac accggcgagt ccgcagtgcg cgagaagcgt ctcaagctgg
     7321 gcatccggcc ggtgttcaag cgcgtggaca cctgcgcggc cgagttcgcc acccgcaccg
     7381 cgtacctgta ttccacctac gaggaggagt gcgaggccga gccgagcgcg cgcaggaagg
     7441 tcatggtgct gggcggcggc ccgaaccgca tcggccaggg catcgagttc gattactgct
     7501 gcgtgcacgc ggcgctggcg ctgcgcgagg agggtttcga gaccatcatg gtcaactgca
     7561 acccggagac cgtctccacc gactacgaca cctccgaccg gctgtatttc gagcccctga
     7621 ccctggaaga cgtgctggag atcgtggaca aggaaaagcc ctggggcgtg atcgtgcagt
     7681 acggcgggca gacgccgctc aagctggcgc gcgacctggc ggccgccggc gtgccgatca
     7741 tcggcaccag cgccgactcg atcgacgtgg ccgaggaccg cgagcgcttc cagcagctgc
     7801 tgcacaagct caagctgcgc cagccgccca accgcaccgc gcgctcggtg gccgaggcgg
     7861 tcaagcacgc cgaggcgatc ggctacccgg tggtggtgcg gccgagctac gtcctgggcg
     7921 ggcgcgcgat ggagatcgtg caccaccagg cggacctcga gcgctacatg aagagcgcgg
     7981 tgaaggtatc gaacgactcg ccggtgctgc tcgaccgcta cctgtcggac gccatcgagg
     8041 tggacgtgga ctgcatcgcc gacggcaccg aagtgctgat cggcggcgtg atggagcacg
     8101 tcgagcaggc gggcgtgcat tcgggcgact cggcctgctg cctgccgccg cattcgctgt
     8161 cggccgagct ggtgcaggag ctgcgccgcc agaccgaggt gatggcgcgc gcgctgcagg
     8221 tctgcggcct gatgaacgtg cagttcgcca tccagcagtc ggagcagggc agcaccgtct
     8281 acgtgctgga agtgaacccg cgcgcctcgc gcaccgtgcc gttcgtttcc aaggccaccg
     8341 gccggccgct ggccaagatc gcggcgttgt gcatggccgg caagacgctc gccgcgcaga
     8401 aggtgcaggg cgacatcacg ccgccgtact actcggtgaa ggaggcggtg ttccccttcg
     8461 ccaagttccc cggcgtcgac accatcctcg gtccggagat gaagtccacc ggcgaggtga
     8521 tgggggtggg cgcgaccttc ggcgaagcct tcgtcaagtc gcaactggcg gcgagcatcc
     8581 ggctgcccac gtccgggcgc gccttcatca gcgtgcgcga cggggacaag cgcgccgcgg
     8641 taggagtggc gcgggacctc gctgcgctgg gcttcacggt gctcgccacg cgcggcaccg
     8701 ccgcggtgct ggccgagcac ggcctggcgg tgaccccggt caacaaggtg gccgaggggc
     8761 gcccgcacat cgtcgacatg atcaagaacg gcgaagtgac cttcatcgcc aatacggtgg
     8821 aggacacgcg caccgcgatc tccgactcgc gcgccatccg cgtcaccgcg ctggcgcagc
     8881 gcgtgaccta ttacacgacc atcgccggcg ccaaggccgc ctgcgccggc atgcagcaca
     8941 tgacgcagct ggagccctac cgcctgcagg acctgcatgc gaaactcggg cttcccaccc
     9001 cggtccgcgc gataggctca cactaatggc caagacaccc atgaccttgc gcggcgccga
     9061 gctgctgcgc gtcgaactgc accgcctgaa gaccgtcgag cggccggcca attcgcaggc
     9121 catcgccgag gcccgggcgc acggcgacct gtcggagaac gccgagtacg acgcggcgcg
     9181 caaccagcag ggcttcatcg aggggcgcat ctcggagctc gaggccaagc tcggcaacgc
     9241 ccagatcatc gacccgaagc tgctggatgc ggacgggcgc tgcgtgttcg gcgcgacggt
     9301 ggacctggag gaaaaaggcg gcaaggcgat cacctaccag atcgtcggcg aggacgaggc
     9361 cgacatcaag gcgcagcgca tctcgatcag ttcgcccatc gcgcgcgcgc tgatcggcaa
     9421 ggaagccggc gacgaggtcg acgtgcacac cccgggcggg gtgaagcgct acgagatcct
     9481 cgacgtgcgc tacgagtagg gcctagcgtc gctcgccggc ccgcctgcgc tgcgggcgca
     9541 ggggtcccgc ggatttcctg cgccgcgcag gcttcctgcg ggcgtccttg cgctcgtccc
     9601 agccggacgc ggcctgggtg cggcgccggg gcgccgcctt cggctccgcc ggcggctttt
     9661 gccggtacac caccaggatc ttgccgatgt gctgcacggc gtgggcgccg gtgcgctcgc
     9721 agatgctcgc cagcgccacc tcgcgctcgt cgcggttgag cggggcggcg cggaccttga
     9781 tcagctcgtg cgcgccgaga gcgcgctcga tctccgcgac caccgcgtcg gtgaggccct
     9841 tctcgcccag ttgcaggatc ggatgcagcg catgcgcgcg ggccttgagc gcctttttct
     9901 gtgcgccggt gatgtggtcc atggcgcgag tataagatcc gcagcgatgg ccaagtccaa
     9961 caaagcctgg ctgcgccgcc acgtcaccga cagctacgtg cgccgggcaa gggaagccgg
    10021 ctaccgctcg cgcgccgcgt acaagctgct cgagtttgtc gccaaggaca ggctcctgaa
    10081 gcccggcatg cgggtcctcg accttggcgc cgcgcccggc ggctggtcgc aggtggcggc
    10141 cgagatcgtg caacccaatg ggaaagtggt ggcggtggac ctgctggaat tgaggccgat
    10201 ccagggagtg acgatctgca agggagattt tcgcgaggcg gacctggatg
//
LOCUS       DASRIE010000065        16222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1638653, whole genome shotgun sequence.
ACCESSION   DASRIE010000065 DASRIE010000000
VERSION     DASRIE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638653"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..2157)
                     /locus_tag="VFB53_02925"
     CDS             complement(16..2157)
                     /locus_tag="VFB53_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZN85717.1"
                     /translation="MNNLRRVQFALSIAVLTLLAAPFAEARVTRIVVDSTAAINNRPT
                     YELLTGRAWGTLDPLDPKNAIITDLEFAPRNADGKVEYVASFRIRKPKDMGQASGVLW
                     HDVPNRGGDVNFPGDSFAAGDAQLLSGWQGDDSGDTAIPANADCLPPCAPAFANHWIK
                     VPVARNPDGSSIAGRVMGRILNASGVDSQPMIVHSNPVPYEPTTLDTTQATLVSRVHE
                     TIEGVVTGEETVPSTEWAWASCSAANPFPGTPDPTQICVNGGFVPNRVYQVVFTTQDP
                     PVLGIGFAAFRDLGSFFKFETQDDFGTANPLASRINRVIARGSSQSGNFLRALLHLGF
                     NQDEAGRQVQDGNWPIIAGRRVALNFRWAMPDGVLKLYEPGSEGPQWWQRYPDRKRGL
                     PPRGILDRCNESGTCPKIVEHFGAAEVWALKLGPEWVGTDPKSDIPLPENVRRYYIPS
                     TRHGGGGGGFNSSLPGSLPAVPNCPSDGYGQGTFADNPVPHAQTVNAIRFHFRNWVMN
                     EVLPPASRYPTLRGAKGERFLVEPTKQAMGFPTIPGIPASAPTGLINPVLDYDWGPEF
                     DPSDASGVPTNMPPPIKQVIPMLVPRVDADGNELGGVPVVLRDAPLGTYMGWNITAAG
                     FHAGKICNYAGGMVPFARTRAEREANNDPRLSLEERYGTHAGYVSAVTAAANKAAEAG
                     FLLAADRQALIDLAAASNVCGGAGGTCNPAP"
     gene            2360..2890
                     /locus_tag="VFB53_02930"
     CDS             2360..2890
                     /locus_tag="VFB53_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZN85718.1"
                     /translation="MRRIAIAAALSALFAAPAAFAADKVRATLTGGEEVPVVSTAAAG
                     EFAGVISDGGDAIDYELSFNGLQGAVQQAHIHLAQPNVNGAIVIWLCQTPTTLAPIAV
                     RDITPECPQSGSVRGLITQANVLAIATQQVATGDIGQVVAAIRAGFAYVNVHTTPSPG
                     GEIRGQIRADSRTAAN"
     gene            3004..3930
                     /locus_tag="VFB53_02935"
     CDS             3004..3930
                     /locus_tag="VFB53_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN85719.1"
                     /translation="MNVTSRQLKAFLLTARHQSFSRAAEQLFITQSGMSMLIRELEAQ
                     LGFRLFERTTRRVTLTPSGAQFLPTAEKSLLGLEAAATSIGRSATAARRRLAIGAMPL
                     IAGKLLPAAIAEYARRDPGLQMELRDGDRKQLIAGVDSGELDMAIGCFLQPVPGLQRK
                     SLYRFTLMLVQPPAEAALLARAPRWDDLADRRLLGGPSESPLQQMIEHQLQRLRRHEA
                     PVMRLNYFETQISMVEAGAGSAVLPSFCLPACRGRKVATAELTDPVVPVDLMQIVRRG
                     RELPCAAEFGAFLASHIAGWMEPWTPGFERAA"
     gene            complement(3932..6298)
                     /locus_tag="VFB53_02940"
     CDS             complement(3932..6298)
                     /locus_tag="VFB53_02940"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HZN85720.1"
                     /translation="MRPLNHTLLFAVALALAAPAAAAPHGQLGLQTLSNRADLISGGD
                     ALVEVLLPADVAPASVRVSVDGRDVTGAFAVRANGRYMGLVTGLALGPNTLLAQVVTP
                     AGSGRPPGREAAHLTVTNHARGGPVFAGAQMQPWICARMVSEPVTVTVPGTSLSAVAM
                     TRVSGLASNPVDEQCNAATRYTYYYQPKAREGTACTFTNTGANRCFEPYPAIEDPATR
                     PLDADIADFTNDRGVTVKSLVRVERGAMNRGIYELVSFYDPLVASAPWAPQPGWNGKL
                     LWRFGAASAYSRFQTPPGTSVWNHNALVRGYMVASASLTDHNTNANDTLGAEAMMMAK
                     EHIVDTYGEIRYTIGDGASGGAIYQHQLAAAYPGLLNGLQPGISYPDTFTTGIEVTEC
                     GLLQDRYYGTRSLVELPATLRAAINGHLTTNFCAVWISSFLARGNPTLAGNCGGGFPT
                     ALTYDPVLRPDGVRCTGADHSRSILGTFPDTDGNTKSPSPFDNEGVQYGLKALQSGVL
                     SPEEFVRLNEGVGSYTMDQGWIPPARKVASNATLRTVYTSGIVSDGRQLAKAAIIDLR
                     SSQNPNGDIHMNWRSWSLRERLDKANGHHDNQVIFAAVGFGAALTLKAFTTLDAWLAG
                     VEADTSGLPVEEKIVRNKPAGTVDICLTTNGATDEQVAANVGLGTPECPVVFQSSPRQ
                     VAGGPLSEDVFKCQLKPLNFSDADYNGAVFTPEQQARLVGVFTAGVCDWSRPGVEQAP
                     ANPWTTFADGPGGRPLGDAPQSIGGCPGFAAGVGNAAGDGCPGGSAGR"
     gene            6549..6965
                     /gene="gspG"
                     /locus_tag="VFB53_02945"
     CDS             6549..6965
                     /gene="gspG"
                     /locus_tag="VFB53_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HZN85721.1"
                     /translation="MPGVVKARGFTLLELLVVVAIIGLLVGYVAPRYFSQVGKSEVTA
                     VRAQIDGFEKALDSYRLDNGRYPTTEQGLNALLTKPADLPKWNGPYLRKAVPLDPWGN
                     PYRYKSPAEKGDYEILSYGKDGQPGGTGDGADISNH"
     gene            6965..8149
                     /locus_tag="VFB53_02950"
     CDS             6965..8149
                     /locus_tag="VFB53_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN85722.1"
                     /translation="MRFEVKAVRDAGAIETFECEAMDALEAARQAAGRGLTVLKVRRT
                     HLFGGSGKGARFPLLLFSQELAALLDAGVPLLEALETLAEKERSSSARGVLAQLTGAL
                     REGRPLSAALAQLPAVFPLLYVATVRASERTGDLAPSILRFVEYQSRLEAVKKKVVNA
                     AIYPTLLLGVGSLVCLFLLLYVVPRFSQIYQERGDELPLFSRLLLAWGNFADANGALI
                     LALAAALAAGVVAAARSGALRARLLALLWAWPAAAERLRTYQLVRFYRTAGMLLSGGI
                     PVVGAFEMAAGVLDARLQGALAEAARAVRQGKPIAAALEAHGLTTPVATRMLAVGERS
                     GNMGAMLERVALFHEEELTRWIEWFSRLFEPLLMTAIGLVIGVIVVLMYMPIFELAGS
                     IR"
     gene            8146..9834
                     /locus_tag="VFB53_02955"
     CDS             8146..9834
                     /locus_tag="VFB53_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN85723.1"
                     /translation="MSAAPQTLFPRFPELLAKARRDAGAAGRSLLETLEEASGFAPEA
                     FVQALGAQLHYRVLAMRELDAYAPDFSLVPYAESAQRGCVALRDAAGALVFAFADPFD
                     AELAAWADARAGAPFEWRLAHRGDLVAFLARHEDSLSALDAVTQGAARADGASDEGED
                     LSLRAIGEDDSEVVRLVRSTLRDALKLGASDIHLESVAGGLVIKFRIDGMLAQVKAVH
                     DAEQAEQAISRIKVLSELDITERRVPQDGRFRARDHGRDVDFRVSIMPSIHGEDAVLR
                     VLDKEALKARVQVLNLENLGFHGEVLKTLRRLSNEPYGMLLVTGPTGSGKTTSLYAAI
                     SEINRGSDKIVTIEDPVEYQLAGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIR
                     DPETANIAVQSALTGHLVFTTVHANNVYDVIGRFMHMNVDLYNFVSALNGVLAQRLLR
                     LLCEECAEPARPAAELLRDSGIAPEDAGGYAFRAGRGCRACRGTGFSGRTAIPELLVL
                     NDELRELIVAREPIRRVKDAARRNGTRSLREAALDMVRAGRTTLEEINRVTFVA"
     gene            9815..10543
                     /locus_tag="VFB53_02960"
     CDS             9815..10543
                     /locus_tag="VFB53_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85724.1"
                     /translation="MSLSWPRRLEVALSPAGAAWRANGARGDCQGAPAAAFAAALAAA
                     PAAAKRVHVTLGGSLVHYLALPWPEALADADEAEGAAYAAHHFRRVFGEAADSWTCRF
                     DAEEGPVRLACAAARALLEELQDAARAAHRRIESVQPFLVGAFNSWRGEFANSGPGAL
                     FVTLEPGRWCAARLAGGEWRALQSGRLEEDVASALVALVARESALAGEDLPVRVYAPA
                     FPALGAQLEGLGDRIKLLHARAAH"
     gene            10524..11060
                     /locus_tag="VFB53_02965"
     CDS             10524..11060
                     /locus_tag="VFB53_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZN85725.1"
                     /translation="MRALRIDFARSAAPTPWAGWGLLAASLAVAALLGAQFRDASTEL
                     ARLEDRGERLRARAARVAAPGANPQSERALRDELQRARAVTQQLTLPWGRLFQAVEGA
                     AGKQVALIALQPESGQNLVRITAEARDLPEAMEFVRRLQATRQLGGVHLASHQVRTED
                     ARRPVRFVVLAALPGEAS"
     gene            11057..11587
                     /locus_tag="VFB53_02970"
     CDS             11057..11587
                     /locus_tag="VFB53_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85726.1"
                     /translation="MKRLRRALRRLGAPGIAGIGVLAACAAMFFSAQLPLEARLAAAR
                     AALAAEEERAARGGAPLTPEAGLAAYYKFFATGVGHERQLERLFALARQSQLQLLQGA
                     YRYQREPGERLARYEVALPVRGSYAQIRRFLAAALNEIPVASLDRIAFERKRAADSQV
                     EANIRFTLFLDAQESR"
     gene            11584..13545
                     /locus_tag="VFB53_02975"
     CDS             11584..13545
                     /locus_tag="VFB53_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN85727.1"
                     /translation="MNRKLLAALALPAAVLLAACAGSRLPEARQSFAEGRLEEGLALL
                     KKDAEARPGEPRPRMDYLRERERIVGQMLADADNARIAGLAIVAEASYKRVLGIEPGN
                     PRAEAGLELLRREVGYSGVLLKAETALRAGDLDGAAALLRKVLGEDPGHRGARALERR
                     VEEQRVRDAMAPPALATAAARKTVSLEFRDVALRSVLEVITRAAGVNFVLDKDVRPDM
                     RATIFVREQSVAEVVEMLLVTNQLAKKVLNENTLLIYPNTPQKQRDYQDLVVKSFYLS
                     NADPKQTVNMIRAIVKTRDLFVDEKVGLVVMRDTPEAVRLAERLIAAQDLADSEVVLA
                     VEVLEVARTKLLDLGLRWPDQVAWSLVGGAPAGAAAGTQGTPGQFTLPEWLNRSSEMV
                     RINVTNPFFILNLRQQDGLTNLLANPRIRVKNREKAKVHIGDRVPVITSTGTSTGFVG
                     QSVSYLDVGLKLEVEPQIYLDDEVGIKVMLEVSNIVREVTAGSATSGSTLTYQIGTRN
                     ANTVLRLRDGETQALAGLITDADRRTIDKVPGLGDFPVLGRLFSSHSEDSGRSEIVLL
                     VTPRVVRSIQRPEARHTEFHSGTEGTVGARGGAPATMGTPPPPPPPPPRLTPPQATPL
                     QPPAPAAPPQPGSPQIFPPGTPIKPPADG"
     gene            13551..14021
                     /locus_tag="VFB53_02980"
     CDS             13551..14021
                     /locus_tag="VFB53_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN85728.1"
                     /translation="MRGFTLIEMVIALAILGVLASVALPLTELAVTRAKETELRRSLR
                     DIRTAIDAYKQAVDEGRLPRAPGESGYPKKLEVLAEGAKDLKNPRGEMIYFLRRIPRD
                     PFAEEAGANQGAADTWGKRSYASPPQEPQEGKDVYDVYSRSARTGLNGLRYRDW"
     gene            14024..14407
                     /locus_tag="VFB53_02985"
     CDS             14024..14407
                     /locus_tag="VFB53_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN85729.1"
                     /translation="MRVWTTTRGFTLVELLVVLAIIGVLLGIALPRYFGSEDRAKEAV
                     LKENLYRMREAIQHYYADKGKYPEQLEALAAEKYLRAVPLDPLTERADTWVVVGPPEP
                     GKGGVYDVKSGAPGAGADGRPFSEL"
     gene            14408..14884
                     /locus_tag="VFB53_02990"
     CDS             14408..14884
                     /locus_tag="VFB53_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN85730.1"
                     /translation="MRERGFSYLGVLFVVALMGILLAAAGEAWHTQAMREREAELLFV
                     GDQYRKAITRYHAAGGVYPRELADLLKDSRRPDTQRYLRKLYADPVTGKNEWGLVKSA
                     DGGIAGVHSLSEAKPFKETGFRQEYAGFEGRKKYSEWQFALVPPAAAKGEKGGQVR"
     gene            14868..16145
                     /gene="fahA"
                     /locus_tag="VFB53_02995"
     CDS             14868..16145
                     /gene="fahA"
                     /locus_tag="VFB53_02995"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HZN85731.1"
                     /translation="MVRSARRAAKSWVESANDPKCDFPLQNLPYGVFRRRGSKESPRG
                     GVAIGDQVLDLAAVGINTGPNLNRLAAAGRNVWRELRRVLTLGLSDPAQAKRLSRFLV
                     PMKKAEMFLPVAIGDYSDFYAGIHHALAIGRMFRPDNPLLPNYKWVPIGYHGRASSVV
                     VSGTPVVRPHGQTKAAEAAAPSFGPAQRLDYEAELGFVIGPGSRLGKPVGIRNALEHV
                     FGVVLLNDWSARDVQAWEYQPLGPFLSKSFATTISPWIVTFEALEPFRCAAFERPSGD
                     PRPLPYLFDTNDQEKGGLEIGLEMYLSSETMRKAKAPPLRLSRGNARDAYWTAAQMLA
                     HHSSNGCNLQPGDLLGSGTLSGATPESAGSLMELTQGGKVPLQFPGGESRIFIEDGDE
                     VTMRGRCERQGAAPIGWGECTGAVRAARTKRQH"
BASE COUNT         2364 a         5740 c         5739 g         2379 t
ORIGIN      
        1 ctcatgattt tctcctcaag gcgccggatt gcacgtcccg ccggcgccgc cgcatacgtt
       61 gctcgccgcg gccaggtcga tcagcgcctg gcggtcggcg gcgagaagga agccggcctc
      121 ggcggccttg tttgccgcgg ccgtcaccgc agacacgtag cccgcgtgcg tgccgtagcg
      181 ctcctcgagc gacaggcgcg ggtcgttgtt cgcttcgcgc tcggccctgg tgcgcgcgaa
      241 cgggaccatg ccgccggcgt aattgcagat cttgcccgcg tggaacccgg ccgccgtgat
      301 gttccagccc atgtaggtgc cgagcggcgc gtcgcgcagc acgaccggaa cgccgcccag
      361 ctcgttgccg tccgcgtcca cccgcggcac gagcatcggg atcacctgct tgatgggcgg
      421 cggcatgttc gtcggcacgc ccgaggcgtc gctgggatcg aactcgggac cccagtcgta
      481 atccagaacg ggattgatca ggccggtcgg cgccgaggcg ggaatgcccg ggatggtggg
      541 gaatcccatc gcctgcttcg tcggctcgac caggaagcgc tcgcctttgg cgccgcgcag
      601 cgtcgggtag cggctggcag gcgggagcac ctcgttcatg acccagttgc ggaaatggaa
      661 ccggatcgca ttcaccgtct gcgcgtgggg gacagggttg tccgcgaacg tcccctggcc
      721 gtagccgtcg ctgggacaat tgggcaccgc ggggagactg cccggcaggc tgctgttgaa
      781 tccgccgcct ccgccgccgt gcctggtgct gggaatgtaa tagcggcgca cgttctcggg
      841 cagcgggatg tcgctcttcg ggtccgtccc gacccattcc gggccgagct tcagcgccca
      901 gacttccgcc gcgccgaagt gctcgacgat cttcgggcag gtgccgctct cgttgcagcg
      961 gtcgaggatg ccgcgcggcg gcagcccgcg cttgcgatcg gggtaccgtt gccaccactg
     1021 cggcccttcg ctgccgggct cgtagagctt caacacgccg tcgggcatcg cccagcggaa
     1081 attgagcgcc acgcgccggc cggcgatgat cggccagttg ccgtcctgca cctggcgccc
     1141 ggcctcgtcc tggttgaacc caaggtgcag caacgcgcgc aggaagttgc ccgactgcga
     1201 gctgccgcgg gcaatgacac ggttgatcct ggacgcgagc ggattcgccg tgccgaagtc
     1261 gtcctgcgtt tcaaatttga agaacgaacc caggtcgcgg aacgcggcga agccgatgcc
     1321 gaggacggga ggatcctgtg tcgtgaagac cacctgatag acacggttgg gcacgaagcc
     1381 gccgttgacg cagatctgcg tcgggtcggg agtgccgggg aaggggttgg ctgcgctgca
     1441 gctcgcccag gcccattcgg tgctcgggac cgtctcctcg ccggtgacca cgccctcgat
     1501 cgtctcgtgc acgcggctga ccagcgtcgc ctgcgtcgta tccagcgtcg tcggctcgta
     1561 cggcaccgga ttggagtgca cgatcatcgg ctgcgaatcg acgccgctgg cgttgaggat
     1621 ccgccccatc acgcgcccgg cgatcgacga cccgtcggga ttcctcgcca ccggcacctt
     1681 tatccagtgg ttggcgaacg ccggcgcgca gggcggcagg caatccgcgt tcgccggaat
     1741 cgcagtgtct cctgaatcgt cgccctgcca accgctcagc aactgcgcgt cgcccgcggc
     1801 gaacgaatcg ccgggaaagt tgacgtcgcc gccgcgattc ggaacgtcgt gccacagcac
     1861 gccgctcgcc tggcccatgt ccttcggctt gcggatgcgg aagctcgcga cgtactcgac
     1921 cttgccgtcg gcgttgcgcg gcgcgaactc caggtcggtg atgatcgcgt tcttcgggtc
     1981 gagcggatcg agcgtgcccc aggcgcgccc ggtgagcagc tcgtaggtcg gtcggttgtt
     2041 gattgcggca gtgctgtcga ccacgatgcg cgtgacgcgg gcctcggcaa agggcgcggc
     2101 aaggagcgta agtaccgcga tggagagagc gaactgcaca cgacgcaggt tgttcatggc
     2161 gcgcctcctg ttttccccaa tggaagcaca gcgcaccatg cgcatacatc ggacattctt
     2221 cttgcattca tcacgcgcgc ttctttgtcg cgaccccccg acagcgatct tcgagtgcga
     2281 acgggaatag ccgcaaaact gtcgctgttc cgcgccagaa gaggatcgct tttccaccac
     2341 gcgcgaaagg agaacgctca tgcgcagaat cgccatcgcc gccgcactgt ccgcgctatt
     2401 cgccgcccct gcggcattcg ccgccgacaa agtgcgcgcc accctcacgg gcggcgaaga
     2461 ggtgcccgtt gtgtccaccg ccgccgccgg cgagttcgcc ggagtgatca gcgacggcgg
     2521 ggacgcaatc gactacgagc tgagcttcaa cggcctgcag ggtgcggtgc aacaggcgca
     2581 catccatctc gcccaaccga atgtgaatgg agccatcgtg atctggctct gccagacgcc
     2641 gaccaccctg gcgccgatcg cggtccgcga tatcactccc gaatgcccgc aatcgggcag
     2701 cgtgcgcgga ctcatcacgc aggcgaacgt cctcgccatt gcgacgcagc aagtcgccac
     2761 gggcgacatc ggccaggtgg tcgccgcgat tcgcgcgggt tttgcgtatg tgaacgttca
     2821 cacgacgccc tcgcccggcg gcgagattcg cggacagatc cgcgccgact cgcgcaccgc
     2881 agcaaactag gcgggaatct tgctccgcag catttttgat gttccagcaa tcgcactata
     2941 ggaaaaagga ctatggacaa gcgccctgac acttcctaaa gtaatttttc atcacgagcg
     3001 ctgatgaacg tcacctcgcg ccagctgaaa gccttcctcc tgacggcacg ccaccagagt
     3061 ttttcgcgcg ccgccgagca gttgttcatc acgcaatccg gcatgagcat gctgatccgc
     3121 gagctcgagg cccagctcgg cttccgcctg ttcgagcgca ccacgcgccg cgtgacgctc
     3181 acgccttcgg gcgcgcagtt cctgcccacc gccgagaaaa gccttctcgg cctggaagcg
     3241 gcggcgacca gcatcgggcg ctcggccacc gccgcgcggc ggcggctcgc catcggcgcc
     3301 atgccgctca tcgccggcaa gctcctgccc gcggcgatcg ccgagtacgc caggcgcgat
     3361 ccggggttgc agatggagtt gcgcgacggc gaccgcaagc agctcatcgc gggggtggac
     3421 tcgggagagc tggacatggc gatcggctgc ttcctgcagc cggtgcccgg cttgcagcgc
     3481 aagtcgctgt accgcttcac gctgatgctg gtgcagccgc ccgccgaggc ggcgctgctc
     3541 gcgcgcgcgc cgcgctggga cgatctcgcc gaccggcggc ttctcggcgg gccgtccgag
     3601 agcccgttgc agcaaatgat cgagcaccag ctgcagcgcc tgcgccggca cgaggccccg
     3661 gtgatgcggc tcaactactt cgagacgcag atctccatgg tggaagccgg cgcgggcagc
     3721 gccgtgctgc cgagcttctg cctccccgcc tgccgcggcc gcaaggtggc gacggcagag
     3781 ctcaccgacc cggtggtgcc ggtcgacctg atgcagatcg tgcgccgcgg gcgcgagctg
     3841 ccgtgcgccg cggagttcgg cgccttcctc gccagccaca tcgccggctg gatggagccc
     3901 tggacgcccg gattcgagcg cgcggcctga gctagcgccc ggcgctcccg cccgggcagc
     3961 cgtcgccagc cgcgttgccc acgcccgcgg caaagccggg gcagccgccg atcgactgcg
     4021 gcgcatcgcc cagcggccgc ccgcccgggc cgtcggcaaa ggtggtccag gggttcgccg
     4081 gcgcctgctc cacgccgggc cgcgaccagt cgcacacgcc cgcggtgaag acgccgacca
     4141 ggcgcgcctg ctgctccggc gtgaacaccg cgccgttgta gtccgcgtcg ctgaagttga
     4201 gcggcttcag ctggcacttg aacacgtcct cggacaaagg cccgcccgcc acctggcgcg
     4261 gcgagctctg gaacaccacc gggcattccg gggtgcccag cccgacgttg gcggcgacct
     4321 gctcgtcggt ggcgccattg gtggtgaggc agatgtccac cgtgcccgcg ggcttgttgc
     4381 gcacgatctt ctcctcgacc ggcaggccgc tcgtgtcggc ttcgaccccg gccagccacg
     4441 cgtcgagcgt ggtgaaagcc ttcagcgtca gcgccgcgcc aaagcccacc gcggcgaaga
     4501 tcacctggtt gtcgtggtgc ccgttcgcct tgtccaggcg ctcgcgcaat gaccaggagc
     4561 gccagttcat gtggatgtcg ccgttcgggt tctggctgct acgcaggtcg atgatcgccg
     4621 ccttggcgag ctgccggccg tcgctcacga tgccgctcgt gtagaccgtg cgcagcgtag
     4681 cgttggacgc gaccttgcgc gccggcggga tccagccctg gtccatggtg tagctgccga
     4741 cgccctcgtt caggcgcacg aactcctcgg ggctgaggac gcccgattgc agcgccttca
     4801 ggccgtattg cacgccctcg ttgtcgaagg gcgaaggcga cttggtgttg ccgtccgtgt
     4861 ccgggaaggt gccgaggatg ctgcgcgaat ggtcggcgcc cgtgcagcgc acgccgtcgg
     4921 gccgcagcac cgggtcgtag gtgagcgcgg tcgggaagcc gccgccgcag ttgcccgcga
     4981 gcgtcgggtt gccgcgcgcc aggaaggacg agatccacac cgcgcagaaa ttggtggtga
     5041 ggtggccgtt gatcgcggcg cgcaacgtgg ccggcagctc gacgagcgag cgcgtgccgt
     5101 aatagcggtc ctgcagcagc ccgcattcgg tgacttcaat cccggtggtg aaggtgtcgg
     5161 ggtagctgat gcccggctgc aggccgttga ggagccccgg gtaggccgcg gcgagctggt
     5221 gctggtagat cgcgccgccc gaggcgccgt cgccgatggt gtagcggatc tcgccgtagg
     5281 tgtcgacgat gtgctccttg gccatcatca tggcctcggc gcccagcgtg tcgttggcgt
     5341 tggtgttgtg gtcggtgagc gatgccgaag ccaccatgta gccccgcacg agcgcgttgt
     5401 ggttccacac cgaggtgccg ggaggcgtct ggaagcggct gtaggcgctc gccgcgccga
     5461 agcgccacag gagcttgccg ttccagccgg gctgcggcgc ccagggcgcc gaggcgacca
     5521 ggggatcgta gaagctgacc agctcgtaga tgccgcggtt catcgccccg cgctcgacgc
     5581 gcaccaggct ctttaccgtc acgccgcggt cgttggtgaa gtcggcgatg tcggcgtcca
     5641 gcggccgcgt ggccgggtcc tcgatcgccg ggtagggctc gaagcagcgg ttggcgccgg
     5701 tgttggtgaa ggtgcaggcc gtgccttcgc gcgccttcgg ctggtagtaa taggtgtagc
     5761 gcgtcgctgc gttgcactgc tcgtccaccg ggttggaggc gaggccgctc acccgcgtca
     5821 tcgcgacggc cgagagcgaa gtccccggca ccgtgacggt caccggctcg gagaccatgc
     5881 gcgcgcagat ccagggctgc atctgcgcgc cggcgaacac cggcccgccg cgcgcatggt
     5941 tggtgacggt gaggtgcgcc gcctcgcgtc ccggcggtcg gccggaaccg gccggagtga
     6001 cgacctgcgc gagcagcgtg ttcggcccga gcgcgaggcc ggtcaccaag cccatgtagc
     6061 ggccgttcgc gcgcaccgcg aaagcgcccg tcacgtcgcg cccgtccacg ctcacccgca
     6121 cgctcgccgg ggcgacgtcc gcgggcagca gcacctcgac cagggcgtcg ccgccggaga
     6181 tgaggtcggc gcggttggag agcgtctgca ggccgagctg gccgtgcggc gcggcggcgg
     6241 ccggcgcggc caaagcgagg gcaaccgcga acagcagcgt atggttcagt gggcgcatgg
     6301 tcgtcttccc ctccggtgga cggggcattt ttccattgcg gatcaggacg attagcgaaa
     6361 tccactcatc aattcatcag caatccggat gtatcgggcg ccggtttcct tcgtgtccaa
     6421 ggttgcaggc tgatgacgcc gcacccccgg tttcacgggc tgcccggacc atgcgcgcct
     6481 ctcgccggcg aggaggtttt catcacccaa tccggcgtaa tatcaacgcc caaggagcac
     6541 tttccgagat gcccggcgta gtcaaggcgc gcggcttcac gctgctggag ctgctggtgg
     6601 tggtggcgat catcgggctg ctggtgggct acgtcgcgcc ccgctacttt tcccaggtgg
     6661 gcaagtcgga ggtgaccgcg gtgcgcgcgc agatcgacgg cttcgagaag gcgctggaca
     6721 gctaccgcct cgacaacggc cgctacccca ccaccgagca gggcctgaac gcgctgctga
     6781 ccaagcccgc cgacctgccg aagtggaacg ggccctacct caggaaggcg gtgccgctcg
     6841 atccctgggg caacccctac cgctacaagt cgccggcgga aaaaggcgac tacgagatcc
     6901 tgtcctacgg caaggacggc cagcccggcg gcacgggcga cggcgccgac atcagcaacc
     6961 actgatgcgc ttcgaggtga aagccgtgcg cgacgccggc gccatcgaga ctttcgagtg
     7021 cgaggcgatg gacgcgctcg aagccgcgcg ccaggccgcc ggccgcggcc tcaccgtgct
     7081 gaaggtgcgg cgcacgcatc tcttcggcgg ctccggcaag ggcgcgcgct tcccgctgct
     7141 cctctttagc caggagctcg ccgcgctgct cgatgcgggg gtgccgctcc tcgaagccct
     7201 ggagacgctc gccgagaagg agcgctcctc gtccgcgcgc ggcgtgctcg cgcagctgac
     7261 cggcgccctg cgcgagggcc gcccgctctc ggcggcgctc gcgcagctgc cggcggtgtt
     7321 cccgctgctc tacgtcgcca ccgtgcgcgc gagcgagcgc accggcgacc tcgcgccctc
     7381 gatcctgcgc ttcgtcgagt accagtcgcg cctggaagcg gtgaagaaga aggtggtgaa
     7441 cgccgccatc taccccacgc tcctcctcgg cgtggggagc ctggtgtgcc tgttcctcct
     7501 gctctacgtg gtgccgcgct tcagccagat ctaccaggaa cgcggcgacg agctgccgct
     7561 cttctcgcgg ctcctcctcg cctggggcaa tttcgccgac gccaacggcg cgctgatcct
     7621 cgccctcgcc gccgcgctcg ccgccggcgt cgtcgccgcg gcgcgcagcg gcgcgctgcg
     7681 cgcgcgcctc ctcgcgctcc tctgggcctg gccggcggcg gccgagcgcc tgcgcaccta
     7741 ccagctggtg cgcttctacc gcaccgcggg gatgctgctc tccggcggca tcccggtggt
     7801 cggcgccttc gagatggccg ccggggtgct cgatgcgcgc ttgcagggcg cgctcgccga
     7861 ggcggcgcgc gcggtgcgcc agggcaagcc gatcgccgcg gcgctcgaag cgcacggcct
     7921 caccacgccg gtggccacgc gcatgctggc ggtgggcgag cgctcgggca acatgggcgc
     7981 gatgctcgag cgcgtggcgc tcttccacga ggaggagctc acgcgctgga tcgagtggtt
     8041 ctcgcgcctg ttcgagccgc tgctgatgac cgccatcggc ctggtgatcg gcgtgatcgt
     8101 ggtcctcatg tacatgccga tcttcgaatt ggcggggagc atccggtgag cgccgcgccg
     8161 cagaccctgt tcccgcgctt tcccgagctg ctcgccaagg cgcggcgcga cgcgggcgcc
     8221 gccgggcgga gcctcctgga gacgctggag gaggcgagcg gcttcgcccc ggaagccttc
     8281 gtgcaggcgc tcggcgcgca gctgcactac cgcgtgctcg ccatgcggga gctcgacgcg
     8341 tacgcgcccg acttttcgct ggtgccttac gcggagtccg cgcagcgcgg ctgcgtggcg
     8401 ctgcgcgacg ccgcgggcgc gctggtgttc gccttcgccg accccttcga cgccgagctc
     8461 gccgcctggg ccgacgcgcg cgccggcgcc cccttcgagt ggcgcctcgc gcaccgcggc
     8521 gacctggtgg cgttcctcgc gcgccacgag gattcgctct ccgcgctgga tgcggtgacg
     8581 cagggcgccg cgcgcgccga cggcgcgagc gacgagggcg aggacctgtc gctgcgcgcc
     8641 atcggcgagg acgacagcga ggtcgtgcgc ctggtgcggt cgaccctgcg cgacgccctc
     8701 aagctcggcg cgagcgacat ccacctggag agcgtggcgg gcggcctggt gatcaagttc
     8761 cgcatcgacg gcatgctcgc gcaggtgaag gcggtgcacg acgccgagca ggccgagcag
     8821 gcgatctcgc gcatcaaggt gctctcggaa ctcgacatca ccgagcggcg cgtgccgcag
     8881 gacgggcgct tccgcgcgcg cgaccacggc cgcgacgtcg atttccgcgt ctccatcatg
     8941 ccgagcatcc acggcgagga cgcggtgctg cgcgtgctgg acaaggaggc gctgaaggcg
     9001 cgcgtgcagg tgctcaacct ggagaacctc ggcttccacg gcgaggtgct caagacgctg
     9061 cggcggctct cgaacgagcc ctacggcatg ctgctcgtca ccgggccgac gggcagcggc
     9121 aagacgacat ccctctacgc cgcgatctcg gaaatcaacc gcggctcgga caagatcgtc
     9181 accatcgagg atccggtgga gtaccagctc gccggcgtgc tgcagatccc ggtgaacgag
     9241 aaaaagggcc tcaccttcgc gcgcggcctg cgctcgatcc tgcgccacga ccccgacaag
     9301 atcatggtgg gcgagatccg tgacccggag accgccaaca tcgcggtgca atcggcgctc
     9361 accgggcacc tcgtgttcac caccgtgcac gccaacaacg tctacgacgt gatcgggcgc
     9421 ttcatgcaca tgaacgtgga cctgtacaac ttcgtctcgg cgctgaacgg cgtgctggcg
     9481 cagcgcctgc tgcgcctgct gtgcgaggaa tgcgccgagc cggcgcgccc ggcggcggaa
     9541 ctgctgcgcg actcaggcat cgcgcccgag gacgccggcg gctacgcctt ccgcgccggg
     9601 cgcggctgcc gcgcctgccg cggcaccggt ttcagcggcc gcaccgcgat tccggagctc
     9661 ctcgtgctga acgacgagct gcgcgagctg atcgtggcgc gcgagccgat ccggcgcgtg
     9721 aaggacgccg cgcgccgcaa cggcacgcgc agcctgcgcg aggcggcgct cgacatggtg
     9781 cgcgcggggc gcaccacgct ggaggagatc aaccgtgtca ctttcgtggc ctaggcggct
     9841 cgaggtcgcg ctgtccccgg cgggcgccgc ctggcgcgcg aacggcgcga gaggcgattg
     9901 ccagggcgcg cccgcggcgg ccttcgccgc ggcgctcgcc gccgcgcccg cggcggcgaa
     9961 gcgcgtccac gtgacgctcg gcggcagcct ggtgcattac ctagccctcc cctggcccga
    10021 ggcgctcgcc gacgccgacg aagcggaagg cgcggcctac gccgcgcacc acttccgccg
    10081 cgtgttcggc gaggcggccg actcgtggac ctgccgcttc gatgccgaag aaggccccgt
    10141 gcgcctcgcc tgcgccgcgg cccgcgccct gctggaagaa ctgcaggacg cggcgcgcgc
    10201 ggcgcaccgg cgcatcgaat cggtgcagcc cttcctcgtg ggcgccttca actcctggcg
    10261 cggcgagttc gcaaactcgg gtccgggcgc gctcttcgtg accctcgagc ccgggcgctg
    10321 gtgcgcggcc cggctcgcag gcggcgagtg gcgcgcgctg caaagcggcc gcctggagga
    10381 ggacgtcgcc tccgccctcg tcgccctggt ggcgcgcgag agcgcgctcg cgggcgagga
    10441 cctgccggtg cgcgtctacg cgcccgcctt cccggcgctc ggtgcgcagc tcgaggggct
    10501 gggcgaccga atcaagcttc tgcatgcgcg cgctgcgcat tgatttcgcg cgctccgccg
    10561 cgccgacgcc ctgggcgggc tggggcctgc tcgcggcctc gctcgccgtc gccgccctgc
    10621 tcggcgcgca gttccgcgac gcgagcaccg agctggcgcg cctggaggac cgcggcgagc
    10681 ggctgcgcgc gcgcgccgcg cgcgtggcgg cgccgggcgc caatccgcag tccgagcgcg
    10741 cgctgcgcga cgagctgcag cgcgcgcgcg ccgtgacgca gcagctcacc ctgccctggg
    10801 ggcgattgtt ccaggcggtg gagggcgccg ccggcaagca ggtggcgctc atcgcgctgc
    10861 agcccgagag cggccagaac ctggtgcgca tcaccgccga ggcgcgcgac ctgcccgagg
    10921 cgatggagtt cgtgcgccgc ctgcaggcca cgcggcagct cggcggcgtg cacctcgcca
    10981 gccaccaggt gcgcaccgag gacgcgcgcc gcccggtgcg cttcgtggtg ctcgccgcgc
    11041 tccccggaga ggcctcgtga agcggctgcg gcgcgcgctg cggcgcctcg gcgcgccggg
    11101 catcgccggc atcggcgtgc tggcggcctg cgccgcgatg ttcttctcgg cgcagctgcc
    11161 gctcgaggcg cgcctcgccg cggcgcgcgc cgcgctcgcc gcggaggagg agcgcgccgc
    11221 gcgcggcggc gcgccgctca ccccggaggc gggcctcgcc gcctactaca agttcttcgc
    11281 caccggcgtg ggccacgagc ggcagctcga gcgcctgttc gcgctcgcgc ggcaaagcca
    11341 gctgcagctg ctgcagggcg cctaccgcta ccagcgcgag cccggcgagc ggctcgcgcg
    11401 ctacgaggtc gcgctgccgg tgcgcggcag ctacgcgcag atccggcgct tcctcgccgc
    11461 cgcgctgaac gagatcccgg tggcctcgct cgaccgcatc gccttcgaac gcaagcgcgc
    11521 cgccgacagc caggtggagg ccaacatccg ctttaccctt ttcctcgacg cgcaggagtc
    11581 ccgatgaacc gcaagctcct cgcagcgctc gccctccccg ccgcagtcct cctcgccgcc
    11641 tgcgccggca gccgcctgcc cgaggcgcgc cagtccttcg ccgaggggcg cctggaggaa
    11701 ggcctcgccc tgctgaagaa ggacgccgag gcgcggcccg gcgagccgcg gccgcgcatg
    11761 gactacctgc gcgagcgcga gcgcatcgtc ggccagatgc tcgccgacgc cgacaacgcg
    11821 cgcatcgccg gcctcgccat cgtcgccgag gcctcctaca agcgcgtgct cggcatcgag
    11881 ccgggcaacc cgcgcgccga ggccgggctg gagctcttgc ggcgcgaggt gggctacagc
    11941 ggggtgctgc tgaaggccga gaccgccctg cgcgccggcg acctggacgg cgccgcggcg
    12001 ctgctgcgca aggtgctcgg cgaggacccg gggcaccgcg gcgcgcgcgc cctggagcgg
    12061 cgcgtcgagg agcagcgcgt gcgcgacgcg atggcgccgc ccgcgctcgc caccgccgcg
    12121 gcgcgaaaga cggtgtcgct cgaattccgc gacgtggcgc tgcgctcggt gctggaggtg
    12181 atcacgcgcg ccgccggcgt caacttcgtg ctcgacaagg acgtgcgccc cgacatgcgc
    12241 gccaccatct tcgtgcgcga gcagagcgtc gccgaggtgg tggagatgct gctggtgacc
    12301 aaccagctgg cgaagaaggt gctgaacgag aacaccctgc tcatctaccc caacacgccg
    12361 cagaagcagc gcgactacca ggacctggtg gtgaagagtt tctacctgtc gaatgccgac
    12421 cccaagcaga cggtgaacat gatccgcgcc atcgtcaaga cgcgcgatct gttcgtcgac
    12481 gagaaggtcg gcctggtggt gatgcgcgac acgcccgagg cggtgcgcct cgccgagcgg
    12541 ctgatcgcgg cgcaggacct cgccgactcc gaggtggtgc tggcggtgga ggtgctggag
    12601 gtggcgcgca ccaagctcct cgacctcggc ctgcgctggc ccgaccaggt ggcctggagc
    12661 ctggtgggcg gcgcgcccgc gggcgcggcc gccggcaccc agggcacgcc ggggcaattc
    12721 accctcccgg agtggctcaa ccgctcctcg gagatggtgc gcatcaacgt caccaacccg
    12781 ttcttcatcc tcaacctgcg gcagcaggac ggcctcacca acctgctcgc caacccgcgc
    12841 atccgcgtga agaaccgcga gaaggcgaag gtgcacatcg gcgaccgggt gccggtgatc
    12901 acctccaccg gcacctcgac cggcttcgtc gggcagtcgg tgtcctacct cgacgtgggc
    12961 ctgaagctgg aagtggagcc gcagatctac ctcgacgacg aggtcggcat caaggtgatg
    13021 ctggaagtga gcaacatcgt gcgcgaggtc accgccggca gcgccacctc gggcagcacg
    13081 ctcacctacc agatcggcac ccgcaacgcc aacaccgtgc tgcgcctgcg cgacggcgag
    13141 acgcaggcgc tcgccggcct gatcaccgac gccgaccggc gcaccatcga caaggtgccg
    13201 ggcctcggcg atttcccggt gctggggcgg ctgttcagca gccacagcga ggacagcggc
    13261 cgcagcgaga tcgtgctcct cgtgacgccg cgcgtggtgc gctcgatcca gcggcccgag
    13321 gcgcgccaca ccgaattcca ttccggcacc gagggcacgg tgggcgcgcg cggcggcgcc
    13381 ccggcgacga tgggcacgcc gccgcccccg cctcccccgc cgccgcgcct gaccccgccc
    13441 caggcgacgc cgctccagcc gccggctccc gccgcgccgc cgcagccggg ctcgccgcag
    13501 atcttcccgc cgggcacgcc gatcaagccg ccggccgacg gctgatgcgc atgcgcggct
    13561 tcacgctgat cgagatggtg atcgcgctcg cgatcctcgg cgtgctcgcc tcggtggcgc
    13621 tgccgctcac cgagctggcg gtgacgcgcg cgaaggaaac ggagctgcgc cgcagcctgc
    13681 gcgacatccg caccgcgatc gacgcctaca agcaggcggt ggacgagggg cgattgccgc
    13741 gcgcgcccgg cgagtcgggc tacccgaaga agctggaagt cctcgccgag ggcgccaagg
    13801 acctgaagaa cccgaggggc gagatgatct acttcctgcg gcgcatcccg cgcgacccct
    13861 tcgccgagga ggccggcgcg aaccagggcg ccgccgacac ctggggcaag aggagctacg
    13921 ccagtccccc gcaggagccg caggagggca aggacgtgta cgacgtgtac agccgctcgg
    13981 cgcgcacggg cctgaacggc ctgcgctacc gcgactggta gccatgcgag tctggacgac
    14041 aacccgcggc ttcacgctcg tggaactcct ggtggtgctc gccatcatcg gcgtgctgct
    14101 cggcatcgcg ctgccgcgct acttcggcag cgaggaccgc gccaaggagg cggtgctcaa
    14161 ggaaaacctg taccgcatgc gcgaggcgat ccagcactac tacgccgaca aggggaagta
    14221 ccccgaacag ctcgaggcgc tcgccgcgga gaagtacctg cgcgcggtgc cgctcgaccc
    14281 gctcaccgag cgcgccgaca cctgggtggt ggtgggcccg cccgagccgg gcaagggcgg
    14341 cgtgtacgac gtgaagagcg gggcccctgg cgcgggcgcc gacggcaggc cgttctcgga
    14401 gctctagatg cgcgagcgcg gcttttccta cctcggcgtg ctcttcgtgg tggcgctgat
    14461 gggcatcctg ctcgccgcgg cgggcgaggc ctggcacacg caggcgatgc gcgagcgcga
    14521 ggccgagctc ctcttcgtcg gcgaccagta ccgcaaggcg atcacgcgct accatgccgc
    14581 cgggggcgtg tacccgcggg aactcgcgga cctcctgaag gactcgcgcc ggccggacac
    14641 gcagcgctac ctgcgcaagc tctacgcgga cccggtgacc gggaagaacg agtggggcct
    14701 cgtgaagtcg gccgacggcg gcatcgccgg ggtgcacagc ctgtcggagg cgaagccctt
    14761 caaggagacc ggcttccgcc aggagtacgc cgggttcgag ggccggaaga agtactcgga
    14821 gtggcagttc gcgctggtgc cgcccgcggc ggccaagggg gagaaaggtg gtcaggtccg
    14881 ctagacgcgc ggcaaaaagc tgggtcgaat cggcgaacga cccgaagtgc gatttcccgt
    14941 tgcagaacct tccctacggc gtgttccgcc gcaggggctc gaaggaatcc ccgcgcggcg
    15001 gggtggcgat cggcgaccag gtgctcgacc tcgccgcggt ggggatcaat accgggccga
    15061 acctgaatcg cctcgccgcc gccgggcgca acgtctggcg cgagctgcgg cgcgtgctca
    15121 cgctcgggct ctcggacccc gcgcaggcga agcgcctctc caggttcctc gtgccgatga
    15181 aaaaggccga gatgttcctc ccggtcgcga tcggcgacta cagcgacttc tacgccggca
    15241 tccatcacgc gctcgccatc gggcgcatgt tccgccccga caatcccctg ctgcccaatt
    15301 acaagtgggt gccgatcggc tatcacggcc gcgcctcgtc ggtggtggtg tcgggcacgc
    15361 cggtggttcg cccgcacggg cagacgaagg cggccgaggc cgcggcgccg agcttcgggc
    15421 ccgcgcagcg cctcgactac gaggcggagc tgggtttcgt catcgggccg gggagccggc
    15481 tggggaaacc ggtgggcatc cgcaacgcgc tcgagcacgt gttcggcgtg gtgctgctga
    15541 acgactggtc ggcgcgcgac gtgcaggcgt gggagtatca gccgctcggg ccgtttctct
    15601 cgaagagctt cgccaccacg atctcgccgt ggatcgtgac cttcgaggcg ctcgaacctt
    15661 tccgctgcgc ggcgttcgag cggccgtccg gggatccgcg gccgttgccg tatttgtttg
    15721 atacaaacga tcaggagaag ggcggcctcg agatcggtct cgagatgtac ctgagcagcg
    15781 agacgatgcg aaaagcgaag gcgccgccgc tgcgtctttc gcgaggcaat gcgcgcgacg
    15841 cgtactggac cgctgcgcag atgctggcgc accatagctc gaacggctgc aatctgcagc
    15901 ccggcgacct gctgggctcc ggtacgctct cgggcgcgac cccggaaagc gccgggtcgc
    15961 tcatggagct tacccagggt ggcaaggtgc cgctgcaatt ccctggcggg gaatcccgaa
    16021 tctttatcga ggacggcgac gaggtgacga tgcgcggccg atgcgagagg cagggcgcgg
    16081 cgcccattgg atggggcgag tgcaccggcg ccgtgcgcgc ggcgcgtaca aagcggcaac
    16141 actaggtttc caaaagcaag caggctggat accgcacgca cctatcgtgc ggggtcgaaa
    16201 ggagggtgtg tgacacccca tc
//
LOCUS       DASRIE010000066        13648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2944463, whole genome shotgun sequence.
ACCESSION   DASRIE010000066 DASRIE010000000
VERSION     DASRIE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944463"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VFB53_03000"
     CDS             <1..103
                     /locus_tag="VFB53_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN85732.1"
                     /translation="EILRHKGALEIELMRTLKAALDPQGLMNPGKVL"
     gene            complement(100..915)
                     /locus_tag="VFB53_03005"
     CDS             complement(100..915)
                     /locus_tag="VFB53_03005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85733.1"
                     /translation="MVARSGAQGHRRLAAALGGLALALQAQAQEAPVHAPFITTPPEV
                     VARMLELARTGPQDFVIDLGSGDGRIVIHAARAHGARGLGLELDAALVALARRRAREA
                     GVGQLAQFEAGDALRADLSRASVVTIYLLPALLDQLRPRLLAQLRPGARVVTHAFGMT
                     GWMPDRSERVRLGARHEGQGDESRIFLFVIPADARGTWRAANGWELRIRQNFQQLEVE
                     ASRAGEAVPVSEARLEGAEISVTGPQVAFRGRVAGASLSGELNGATLAFERVR"
     gene            complement(864..1355)
                     /locus_tag="VFB53_03010"
     CDS             complement(864..1355)
                     /locus_tag="VFB53_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN85734.1"
                     /translation="MRRTVAALLWCCCVAAFAQLPEWSGGPTPALRLPDLEGRLHALE
                     DYRGKVVLINFWATWCEPCREEMPSIERLRASMAGEPFAVLAVNLAEPESRVRGFLNL
                     TPLGFPVLLDPDRTVARAWKARYLPATFIVGPDGRVRYSYFGELDWSRDPVRKAIADL
                     LPR"
     gene            complement(1352..1936)
                     /locus_tag="VFB53_03015"
     CDS             complement(1352..1936)
                     /locus_tag="VFB53_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN85735.1"
                     /translation="MTLHRRDVVQALLAERGGLLVVAGLGSTAWDCTAAGDHDLTFPL
                     WGAMGCAAMLGLGLALAQPQRRVLVVTGDGEMLMGLGSLATIGVQRPANLSVVVIDNE
                     RYGETGMQETHTAHGVDLAAIARAAGFASGVLLRDAAGLGALRRTIHEAPGPNFAQVK
                     VSAEKVPLVLPPREGALLKNRFRAALLGAGAALQ"
     gene            complement(1933..2478)
                     /locus_tag="VFB53_03020"
     CDS             complement(1933..2478)
                     /locus_tag="VFB53_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN85736.1"
                     /translation="MNAPLPAAVDWPLATHQVLARHGVGLVGYVPDAGHKRLIELCQA
                     DARMRTVVMTTEEEGVGLAAGAWLGGVKCALLMQSSGVGNIINALGMTRECRFPLLLL
                     ATMRGENGEFNPWQVPMGQATPGVLRAMGVVVHQAESADAVPGQVDASARIAFGSYCA
                     AAVLISQVVIGIKSFQEQSGR"
     gene            complement(2490..3563)
                     /locus_tag="VFB53_03025"
     CDS             complement(2490..3563)
                     /locus_tag="VFB53_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZN85737.1"
                     /translation="MAHPTRRPPIVGRYTSQEVALAARNSGMPLEALRHEVTPLGLHY
                     CLIHFDIPQLEAQSWRLAVAGLVERPLSLSLEDLRRMPARTRRVTLECAGNGRAQLSP
                     RYPSIPWIEEGVSTADWTGVPLAEVLRAAGLRAAAKEVVFHGADRGIDRATEHDFARS
                     LAPADALQEDVLLAYAMNGAPLPPQHGFPLRLVVPRWYGMASVKWLVRIEAIDHAYDG
                     VQQAHSYHFRSAAGERGEPCRLMRVNSLLVPPGIPDFYTRERVLGAGRVAIFGRAWAG
                     EAPVARVEFGVDGAWRDAALEAPAAPHAWCAWRAQWDAAPGAHELACRATDAAGKRQP
                     LEPPWDTTGFGNNAVQRVAVRVV"
     gene            3610..4440
                     /locus_tag="VFB53_03030"
     CDS             3610..4440
                     /locus_tag="VFB53_03030"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZN85738.1"
                     /translation="MANRTWLEVALNGPWGRERQPGIPVRVTDIVAEGVACAREGAAI
                     VHVHAYDEASGRQRDDADIYARIIEGIRARVDCIVYPTIPFGGEHTAESRFAAVEGLA
                     RRGLLECAVVDPGSVNIDGFVYANPDEHIRHGLGLAARHRFTPGYAIYEPGFLRLGAR
                     LAREAGAPLPIYRFMFSQGFDFGFPPAEYALEAYLRLLADAAPGSPWMIAGLAVDVLP
                     LAATAAMRGGHVRVGLEDAPFGSPRGNLQWVQAAAGAIRSAGGELASVAEVRRALNPH
                     "
     gene            4440..5024
                     /locus_tag="VFB53_03035"
     CDS             4440..5024
                     /locus_tag="VFB53_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZN85739.1"
                     /translation="MDLAQIRAALPRGDLWVFGYGSLMWSPGFPYREHSVGLLRGYHR
                     ALCILSTRYRGTDRRPGLVMGLCRGGSCWGMAFRVPAASVGRALARLWRREMRRKVYA
                     PKLVPVRIARGRRIHALAFIADPAHPQYQREMDLRSRAQLVAQGVGERGRCTDYIRNT
                     LEHMYALGVTDPHLARVLETAEAMRSRADRKRAR"
     gene            complement(4955..6106)
                     /locus_tag="VFB53_03040"
     CDS             complement(4955..6106)
                     /locus_tag="VFB53_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HZN85740.1"
                     /translation="MAFDFDAPVDRAGTWSSRWERYAGRDVIPLWVADTDFRAPDCVL
                     DALGARLRHGVLGYTAAPQELREAIALRMQRLYGWRIETDWIVFLPGVVPGLHLAARH
                     LLPAGGHALVPTPIYHHFKRAVELAPRAHADVPLVLSAGRWVFDEDRLAAAVRPESRL
                     LYLCNPQNPGGTIFTRAELERLAAFAVRHNLVICSDEIHADLLLDAAKPHLPIASLAP
                     EVSRRTVTLASPNKSFNFPGAGCAWAIIEDAALRHAFSVDHHATVHDASAFGYLAALA
                     AYQEGGPWLAAQLEYLRGNRDLVEQAVAGLPGLAMAHVEATYLAWIDAGGLGVKDAQA
                     HFLGHGLALSPGEQFGAPGYVRLNFGTQRARLRSALERMASAVSSTRAR"
     gene            complement(6172..7677)
                     /locus_tag="VFB53_03045"
     CDS             complement(6172..7677)
                     /locus_tag="VFB53_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN85741.1"
                     /translation="MSGLVLVGMCMLVGMIFAIFIGFPISFTLLFLALTFGYFGLGHV
                     VFDLAYFQTIGMMKEELLAAVPLFIFMGFITEQAGLMERLFSAFRMLLAPVRGALFLV
                     VIVTSTVFAMATGIVGAAVTVLGIMASPIMIKTGYDARLAAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYAAAFGPGFLLAGLYLFYLAGRSFINPKLGPPVPKEERIYSV
                     YQILREVLVGVVPLLGLIAATLGTILAGLATPTEASGIGSLGALILAACYRKLTYAGL
                     KRAVLSATATSSMVLLLAVTSNIFGAVFARMGSANWITETLLAMPIPPVAMLVMVVVL
                     IFLLGWPFEWPAVILVFLPIFYPVMDGLKEVLAKSLDIPVDMLMVWFGTVVAVTLQTA
                     YLSPPVAMSAYYLRQVVKEWSLATIYKGMMEFMILQCIAIALVIIFPSIAIWFPQVLQ
                     AEARAVVTEEVEGGASLDDYKQPGMASDKESEEEEGAGGSLEEDEAVKPKK"
     gene            complement(7674..8225)
                     /locus_tag="VFB53_03050"
     CDS             complement(7674..8225)
                     /locus_tag="VFB53_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZN85742.1"
                     /translation="MADAVPKHIRLVRIIDKFTDTTGTWVAWLNLPLVGAISYEVVAR
                     YAFHSPTIWSFDVTYMLYGTIFMLGCAYALHKGAHIRTDFFFEKWSVKTKGIIDSIAY
                     LVFFFPSLLVFLVVSADEGWYAFSIGEVSEQTPWRPLLWPYKWVIPLAVLLLLIQGVS
                     ETIKSVWAARTGIELEHKEKIEI"
     gene            complement(8323..9441)
                     /locus_tag="VFB53_03055"
     CDS             complement(8323..9441)
                     /locus_tag="VFB53_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZN85743.1"
                     /translation="MRQYVRSAVALAAVAAFSAGAQAQTSLKMQATWPASLTLYENFL
                     HFTDRVNKMSGGTLKIEAMPAGQVVPAFEVLDATHKKVLDGAHAWAGYWTGKNKTSIL
                     FTGGPGGTFGMDYIDAMGWLHHGGGLELYQDFYQKELKLNVVVIPILPAGPQAFGWFK
                     RPIKNLADMKGMKCRQTGLAAEVWQRMGMTTVNMPGGEIIPSAQRGVIDCAEWVGGIE
                     DLRLGFQNVWKYHYTPGMHENVTIGELLINGDIWKSLNPAQQTMLQVAANDTFLIWWA
                     KWQRQNADALKELQEKHGVRILRTPGDILVAFLKEWDKLAGEESAKNPFFKKVHESQR
                     SYASLVVPAKRFMFPPYSFAANYYFPEKPAAAAKGKKQ"
     gene            complement(9574..10704)
                     /locus_tag="VFB53_03060"
     CDS             complement(9574..10704)
                     /locus_tag="VFB53_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZN85744.1"
                     /translation="MRTTFRKTLALSVITAALGFAVPAHAQVKTLKMQSTWPASLTLQ
                     DNFRMFADRMDKLTSGQVKIEAMAAGQIVPAFEILDATHKKVIDGGHGVAYYWVGKHK
                     AATLFSATPAGPFGMDHFDFLGWMNEGGGMDLYWEFYRDVLKLNVVGWPIGGSSPQAF
                     GWFKRPIKNLADFKGMKCRQTGIVAEIYQKMGMTTINMPGGEIIPSAQRGVIDCAEWV
                     GGVEDLRLGLPQVWKYHYTPGMHESNSMLELIMNGDVWKGLSAQQQEAVRAATFDTFV
                     RWGIRWQKQNADAIDEMRTKHGTQILRTPPEILVEFMKTWDRMAAEESAKDPFFKKVL
                     DSQRAYAAKVVPAKRFMFPPYSFAANYYFPETKAKPASKAKK"
     gene            complement(10773..11549)
                     /locus_tag="VFB53_03065"
     CDS             complement(10773..11549)
                     /locus_tag="VFB53_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZN85745.1"
                     /translation="MSGYEVYAVKYAHHARTAKENFLGGDPHDGPMPMDYFVWLIRGQ
                     GREIVVDTGFNVAIATKRGRRVTLPVEEGLRRMGVDAAQVKDVAITHLHYDHVGNFDL
                     FPAATFHLQDLEMQYATGRCMAVEAMRHAYELEDVVGMVRRVYAGRVRFHDGEGEIAP
                     GVTLHHVGGHTMGLQIVRVATRRGWVVLASDASHYYANMEQSRPFPIVYNVGDMVEGW
                     HRAQRLAESPAHVIPGHDPLVLARYPAPSKALEGIVARLD"
     gene            complement(11546..12760)
                     /gene="tyrS"
                     /locus_tag="VFB53_03070"
     CDS             complement(11546..12760)
                     /gene="tyrS"
                     /locus_tag="VFB53_03070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZN85746.1"
                     /translation="MRQTAKPPVAEALALIKRGAEELIVEEELARKLARGTPLRVKLG
                     LDPTAPDLHLGHTVVINKLRHFQDLGHQVQFLIGDFTGMIGDPTGKNQTRPPLSREQI
                     LENAKTYRTQAFKILDAERTQILFNSEWSDKLGAEGMIRLAARYTVARILERDDFSRR
                     YKAGQPIAVHELLYPLMQGYDSVAMKSDVELGGTDQKFNLLVGRELQKDYGQEPQCIL
                     TTPLLEGLDGVNKMSKSLGNYVGIAEPPQQIFGKLMSISDELMWRYIELLSFEPMAAV
                     RKWRAEVAAGANPRDVKVRFAREIVERFHSRAAAERAEEEFAQRFRHGQAPEDMPEFT
                     LRSPAGGLAVTQALKQAGLAPSVSEAARNIEQGGVKLDGERLDDKALKLAAGTTVVAQ
                     VGKRKFARIRVV"
     gene            12819..>13648
                     /locus_tag="VFB53_03075"
     CDS             12819..>13648
                     /locus_tag="VFB53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HZN85747.1"
                     /translation="MSQYAPLARSLALLALALSGVVAAFATMAPSAAPGPPPRSAVTE
                     PVALRAGEALLQAPATYLREERFQRGDTLAGLLARLGVAPADAQKLIRVPALRQLRPG
                     HAVSVEIGADGELLRLSYLSGRDRITELRRAGDGFHARELAAQLQAQVLMRAGVIESS
                     LFGAADAAGIPDAVAIQVADIFAGDVDFHRELRRGDRFAVVYEQFALDGVPVRAGRVL
                     AAEFVNRGRTLRAVLYAPERGGSGYYAPDGSNLRKAFLRTPLEYSRISSGFGMRRHPI
                     "
BASE COUNT         2091 a         4862 c         4595 g         2100 t
ORIGIN      
        1 ggagatcctg cgccacaagg gcgccctcga gatcgagctg atgcgcacgc taaaggcggc
       61 gctcgatccg cagggcctga tgaacccggg gaaggtgctc tagcgcacgc gctcgaaggc
      121 cagcgtcgcg ccgttcagct cgccggagag cgaagcgccc gcaacgcggc cgcgaaaggc
      181 gacttgcggc ccggtgacgc tgatctcggc tccctcgagg cgcgcctcgc tcaccgggac
      241 ggcctcgccg gcgcggctgg cctcgacctc cagctgctgg aaattctgcc ggatacgcag
      301 ctcccagcca ttcgccgcgc gccaggtccc gcgcgcgtcc gccgggatca cgaagaggaa
      361 aatccggctt tcgtcgccct ggccctcgtg gcgcgcgccg aggcgcacgc gctcgctgcg
      421 gtccggcatc cagcccgtca tgccgaaggc gtgcgtcacc acgcgcgcgc ccggtcgcag
      481 ctgcgccaac aggcgcgggc gcagctggtc gaggagcgcg ggcagcaggt agatggtgac
      541 gacgctcgcg cgcgacaggt ccgcgcgcag cgcgtcgccc gcctcgaact gcgcgagctg
      601 cccgacgccg gcctcgcgcg cgcgcctgcg cgccagcgcc accagcgccg cgtcgagctc
      661 tagtcccaag ccgcgcgccc cgtgggcgcg agcggcatgg atcacgatgc gcccgtcgcc
      721 cgaacccagg tcgatgacga aatcctgcgg cccggtgcgc gccagctcga gcatgcgcgc
      781 caccacctcc ggcggcgtgg tgatgaacgg cgcgtggacc ggcgcctcct gcgcctgggc
      841 ctgcagcgcc agtgccagcc cgcctagcgc ggcagcaagt cggcgatggc cttgcgcacc
      901 ggatcgcgcg accaatcgag ctccccgaaa tagctgtagc gcacgcggcc atcggggccg
      961 acgatgaagg tcgccggcag gtagcgcgct ttccaggccc gggccaccgt ccgatcgggg
     1021 tcgagcagca ccgggaagcc gagcggcgtc aggttcagga agccgcgcac ccgcgactcc
     1081 ggctcggcga ggttcaccgc cagcactgcg aacggctcgc ccgccatcga ggcgcgcagc
     1141 cgctcgatgg agggcatctc ctcgcggcag ggttcgcacc aggtggccca gaagttgatc
     1201 agcacaacct tgccgcggta gtcctccagc gcgtgcagcc tgccctcgag gtccggcagg
     1261 cgcagcgccg gcgtcgggcc gccgctccac tcgggcagct gcgcgaacgc cgcaacgcag
     1321 cagcaccaca gcagcgccgc gacagtacgc ctcattgcag ggcagcgccc gctccgagca
     1381 gggcggcgcg gaagcggttc ttcagcagcg cgccctcgcg cggcggcagc accagcggaa
     1441 ccttttccgc cgacaccttc acctgcgcga aattcggtcc cggggcttcg tggatcgtcc
     1501 ggcgcagcgc ccccaggccc gcggcatcgc gcagcaggac cccggaagcg aatccggcag
     1561 cgcgcgcaat cgccgccagg tcgacgccgt gcgcggtatg ggtttcctgc atgccggtct
     1621 cgccgtagcg ctcgttgtcg atcaccacca cgctgaggtt cgcgggccgc tgcacgccga
     1681 tggtggcgag cgaccccagg cccatcagca tttcgccgtc gcccgtcacc acgagcacgc
     1741 ggcgctgcgg ctgcgcgagc gccaggccca gccccagcat cgcggcgcag cccatcgcgc
     1801 cccacagcgg gaaggtcagg tcgtggtcgc ccgccgcggt gcagtcccag gcggtcgagc
     1861 ccaggccggc gaccaccagc aggccgccgc gctccgccag cagcgcctgc accacgtcgc
     1921 gccggtgcag cgtcatcggc ccgactgctc ctggaagctc ttgatgccga tcaccacctg
     1981 ggagatcagc acggccgcgg cgcaatagct gccgaaggcg atgcgcgccg aggcgtccac
     2041 ctggccgggc accgcgtcgg cggattccgc ctggtgcacc accacgccca tcgcgcgcag
     2101 cacgcccgga gtcgcctgcc ccatcggcac ctgccaggga ttgaattcgc cattctcgcc
     2161 gcgcatggtg gcgagcagca gcagcgggaa gcggcattcg cgcgtcatgc cgagcgcatt
     2221 gatgatgttg ccgaccccgc tggactgcat cagcagcgcg catttcacgc cgccgagcca
     2281 ggcgccggcg gccagaccca cgccctcctc ttcggtggtc atcactaccg tgcgcatgcg
     2341 tgcatccgcc tggcacaact cgatcaggcg cttgtggccc gcgtccggca cgtagcccac
     2401 caggccgacg ccgtggcgcg cgagcacctg gtgcgtggcg aggggccagt cgacggcagc
     2461 gggcaggggg gcgttcaagg gtggcgattt taaaccacgc gcaccgcgac ccgttgcacc
     2521 gcgttgttgc cgaagccggt ggtgtcccag ggcggctcga gcggctgccg cttgccggcg
     2581 gcgtcggtcg cgcggcaggc gagctcgtgc gcgcccggcg ccgcgtccca ctgcgcgcgc
     2641 caggcgcacc aggcatgcgg cgcagccggc gcttcgagcg cggcgtcgcg ccaggcgccg
     2701 tccacgccga actccacccg ggcgacgggg gcctcgcccg cccaggcccg cccgaaaatc
     2761 gcgacgcggc cggcgccgag cacgcgctcg cgcgtataga agtcgggaat gcccggcggc
     2821 acgaggagcg aattcacgcg catcagccgg cagggttctc cgcgctcgcc cgccgcggac
     2881 ctgaaatggt aggagtgcgc ctgctgcacg ccgtcgtagg catggtcgat ggcctcgatg
     2941 cgcaccagcc atttcacgct cgccatgccg taccagcgcg gcaccaccag gcgcaggggg
     3001 aagccgtgct gcggcggcag cggcgcgccg ttcatggcgt aggcgaggag cacgtcctcc
     3061 tgcagcgcat cggccggcgc gaggctgcgc gcgaaatcgt gctcggtggc ccgatcgatg
     3121 ccccggtccg cgccatggaa caccacttcc ttcgccgctg cgcgcaggcc cgccgcgcgc
     3181 aggacttccg ccagcggcac cccggtccag tccgcggtgg acacgccctc ttcgatccat
     3241 gggatcgagg ggtagcgcgg cgacaactgg gcgcggccgt tgccggcgca ttcgagcgtc
     3301 acgcgccggg tgcgtgccgg catgcgccgc agatcctcca gggacagcga caacggccgc
     3361 tccaccaggc cggccactgc caggcgccag gattgcgctt ccagctgcgg gatgtcgaag
     3421 tggatcaggc aatagtgcag gccgagcggc gtcacctcgt ggcgcagagc ctccagcggc
     3481 atgccgctgt tgcgcgccgc gagcgcgacc tcctgcgagg tatagcggcc gacgatcggc
     3541 gggcggcgcg taggatgggc catggacggg ttgcctgcgg ccgattctat aatgcgctgc
     3601 gaaccattca tggcgaaccg gacctggctc gaagtcgcac tgaacggccc ctggggccgc
     3661 gagcggcaac cgggcatccc cgtgcgcgtc acggacatcg tcgccgaggg cgtggcctgc
     3721 gcgcgcgagg gcgcggccat cgtccacgtg cacgcctacg acgaggcaag cggacgccag
     3781 cgcgacgatg cggacatcta tgcgcgcatc atcgaaggca tccgcgcgcg ggtggattgc
     3841 atcgtctatc cgaccatccc attcggcggc gagcacaccg cagaatcgcg cttcgccgcc
     3901 gtggagggcc tcgcgcgccg cggcctgctc gaatgcgcgg tggtggaccc gggctcggtg
     3961 aatatcgacg gcttcgtcta cgccaacccg gacgagcaca tccgccacgg gctcggcctg
     4021 gcggcgcgcc atcgtttcac gccgggctac gccatctacg agcccggctt cctgcgcctg
     4081 ggcgcgcggc tggcgcggga agcgggcgcg ccgctgccga tctaccgctt catgttctcg
     4141 cagggcttcg acttcggctt cccgcccgcc gagtacgcgc tcgaggccta cctgcgcctg
     4201 ctggccgacg cggcgcccgg atcgccctgg atgatcgccg gcctggcggt ggacgtgctg
     4261 ccccttgccg cgaccgcggc gatgcgcggc gggcacgtgc gcgtcggcct ggaggatgcg
     4321 cccttcggct cgccgcgcgg caacctgcaa tgggtccagg ccgccgccgg cgcgatccgc
     4381 tccgccggcg gcgagctcgc ctcggtcgcc gaggtgcgcc gcgcgttgaa cccgcactga
     4441 tggacctggc gcagattcgc gccgcgctgc cgcgcggcga cctttgggtg ttcggctacg
     4501 gctcgctgat gtggtcgccg ggattcccct accgcgagca ctccgtcgga ctgctgcgcg
     4561 gctaccaccg cgcgctttgc atcctctcca cccgctatcg cggcaccgat cgccgcccgg
     4621 ggctggtgat gggcctgtgc cgcggcggct cctgctgggg catggcgttt cgcgtcccgg
     4681 cagccagcgt cggccgggcg ctcgcccggc tttggcgccg cgagatgcgg cgcaaagtgt
     4741 atgcgcccaa gctcgtgccg gtgcgcatcg cgcgcggcag gcgcatccac gcgctggcgt
     4801 tcatcgccga ccccgcgcac ccgcaatacc agcgcgaaat ggacctgcgc agccgcgccc
     4861 agctggtggc gcagggcgtc ggcgagcggg gccgctgcac cgactacatc cgcaatacgc
     4921 tcgagcacat gtatgcgctc ggcgtcaccg accctcacct cgcccgggtg ctcgagactg
     4981 ccgaggccat gcgctccagg gctgatcgca agcgcgcgcg ctgagtgccg aagttcaacc
     5041 gcacgtagcc cggcgcgccg aactgctccc cgggggagag cgccaggcca tgcccgagga
     5101 agtgcgcctg cgcatccttc acgcccaggc cgccggcgtc gatccaggca aggtaggtcg
     5161 cctccacgtg cgccatcgcc aggcccggca ggcccgccac cgcctgctcc accaggtcgc
     5221 ggttgccgcg caggtactcc agctgcgcgg ccagccaggg tccgccctcc tggtacgccg
     5281 ccagggcggc gaggtagccg aacgcggacg cgtcgtgcac cgtggcgtga tgatcgacgc
     5341 tgaatgcgtg gcgcagcgcg gcatcctcga tgatggccca ggcgcagccc gcgcccggga
     5401 aattgaagct cttgttgggc gaggcgagcg tcaccgtgcg ccgcgacacc tcgggcgcga
     5461 ggctcgcgat cggcaggtgc ggcttggcgg cgtccagcag aaggtcggcg tggatctcgt
     5521 cggagcagat gacgaggttg tggcgcacgg caaaggcagc cagccgctcg agctcggccc
     5581 gggtgaagat cgtgccgccc ggattctgcg gattgcacag gtagagcagc cgcgactccg
     5641 gccgcacggc ggccgccagc cgatcctcgt cgaataccca cctgcccgcg ctcagcacca
     5701 gcgggacgtc ggcatgggcg cgcggcgcga gctccacggc acgcttgaag tggtggtaga
     5761 tgggcgtcgg caccagcgca tggccgcccg cgggcaacag gtggcgcgcg gccaggtgca
     5821 ggcccggcac cacgcccggg aggaacacga tccagtccgt ctcgatgcgc cagccgtaca
     5881 gccgctgcat gcgcaatgcg atggcctcgc gcagctcctg cggggccgcc gtgtatccca
     5941 gcacgccgtg gcgcaggcgc gcgccaagcg cgtcgaggac gcagtccggc gcgcggaagt
     6001 cggtgtcggc gacccacagc gggatcacgt cgcgcccggc gtagcgctcc cagcgcgagg
     6061 accaggtgcc cgcgcggtcc accggcgcgt cgaaatcgaa ggccacgcgt cggttgtagc
     6121 aaaaaaaaac gccgggcgcg aggcccggcg tcccaatcgc ggcgcgtacg gctatttctt
     6181 cggcttgacc gcttcgtctt cttccaggct cccccccgcg ccctcctcct cctcggattc
     6241 cttgtcgctt gccatgcccg gctgcttgta atcgtccagc gaggcgccgc cctcgacctc
     6301 ctcggtcacc actgcgcgcg cctcggcctg cagcacctgc gggaaccaga tcgcgatcga
     6361 cgggaaaatg atcaccaggg cgatcgcgat gcactgcaga atcatgaact ccatcattcc
     6421 cttgtagatg gtcgccagcg accactcctt caccacctgc ctgaggtagt aggccgacat
     6481 ggcgaccggc ggcgacaggt aagcggtctg caaggtcacc gccactaccg tgccgaacca
     6541 caccatcagc atgtccaccg ggatgtccag gctcttggcg agcacttcct tcaggccgtc
     6601 catcaccggg tagaagatcg gcaggaacac caggatcacg gcgggccact cgaagggcca
     6661 gccgaggagg aagatcagca ccaccaccat caccagcatc gccaccggcg ggatcggcat
     6721 ggcgagcaga gtctcggtga tccagttggc cgagcccatg cgcgcgaaca cggcgccgaa
     6781 gatgttcgag gtcacggcga gcagcagcac catgctggag gtcgcggtgg cgctgagcac
     6841 cgcgcgcttc aggcccgcgt aggtgagttt cctgtagcag gcggcgagga tcagcgcgcc
     6901 gagcgagccg atgcccgagg cctcggtcgg ggtcgccagg ccggcgagga tcgtgccgag
     6961 cgtcgcggcg atcaggccga gcagcggcac cacgccgacc agcacctcgc gcaggatctg
     7021 gtagacgctg tagatgcgtt cttcctttgg caccggcggg ccgagcttcg gattgatgaa
     7081 ggagcggccg gccaggtaga agaggtacag gccggcgagc aggaaacccg gcccgaacgc
     7141 cgccgcgtac aggtccgcga ccgacacgcc gagcaccggg cccatgacga tcagcatcac
     7201 cgaaggcggg atcaggatgc ccagcgtgcc ccccgcggtg atcgcgccgg cagcgagcct
     7261 ggcgtcgtag ccggtcttga tcatgatcgg cgaagccatg atgccgagca ccgtcaccgc
     7321 ggcgccgacg atgccggtgg ccatcgcgaa tacggtcgag gtgacgatca ccaccaggaa
     7381 cagcgcgccg cgcaccggcg cgagcagcat gcggaaggcc gagaacagcc gctccatcag
     7441 gcccgcctgc tcggtgatga agcccatgaa gatgaacagc ggcaccgccg ccagcagctc
     7501 ctccttcatc atgccgatgg tctggaaata ggccaggtcg aagaccacgt gcccgaggcc
     7561 gaagtagccg aaggtgagcg cgaggaacag cagggtgaag gagatcggga agccgatgaa
     7621 gatggcgaag atcatcccca ccagcatgca catgccgacc agtaccaggc cgctcatatc
     7681 tcgatcttct ctttgtgctc cagctcgatg ccggtgcgcg cggcccacac gctcttgatg
     7741 gtttccgaca cgccctggat cagcagcagc agcaccgcca gcgggatcac ccacttgtac
     7801 ggccacagca gcggccgcca cggcgtctgc tccgacacct cgccaatgct gaaggcgtac
     7861 cagccttcgt ccgcgctgac gacgaggaac accagcagcg acgggaagaa gaacaccagg
     7921 taggcgatgg agtcgatgat gcccttggtc ttcaccgacc acttctcgaa gaagaagtcg
     7981 gtgcggatgt gcgcgccctt gtgcagcgcg taggcgcagc ccagcatgaa gatcgtgccg
     8041 tacagcatgt aggtgacgtc gaacgaccag atggtcggcg agtggaacgc gtagcgcgcg
     8101 accacctcgt aactgatcgc gccgaccagc ggcaggttca gccacgccac ccaggtgccg
     8161 gtggtgtcgg tgaacttgtc gatgatccgc accaggcgga tgtgcttcgg tactgcgtca
     8221 gccatgcatg cgcccttgag cttgtcagcg cgccgtcagc gacgcgcgcc tgcagacgta
     8281 aaacgggggc ggatgtccgc ccccgtgtcc ggtcgtgatg ccttactgct tcttgccctt
     8341 ggccgctgcc gccggcttct cggggaagta atagttcgcc gcgaacgagt acggcgggaa
     8401 catgaagcgc ttggccggca ccaccagcga ggcgtaggag cgctgcgact catgcacctt
     8461 cttgaagaac gggttcttgg cgctctcctc gccggcgagc ttgtcccatt ccttcaggaa
     8521 cgcgaccagg atgtcgcccg gggtgcgcag gatgcgcacg ccgtgcttct cctgcagctc
     8581 tttcagggcg tccgcgttct gccgctgcca cttggcccac cagatgagga aggtgtcgtt
     8641 ggccgcgacc tggagcatcg tctgctgcgc ggggttgagc gacttccaga tgtcgccgtt
     8701 gatcagcagc tcgccgatgg tgacgttctc gtgcattccg ggcgtgtagt ggtatttcca
     8761 cacgttctgg aagcccaggc gcagatcctc gatgccgccg acccactccg cgcagtcgat
     8821 cacgccgcgc tgcgccgacg ggatgatctc gccgcccggc atgttcaccg tggtcattcc
     8881 catgcgctgc cagacctctg ccgcgaggcc cgtctggcgg cacttcatgc ccttcatgtc
     8941 ggcgaggttc ttgatcggcc gcttgaacca gccgaaggcc tgcgggcctg ccggcaggat
     9001 cgggatcacc accacgttga gcttcagttc cttctggtag aagtcctggt agagttccag
     9061 gccgccgccg tggtgcagcc agcccatggc gtcgatgtaa tccatgccga aggtgccgcc
     9121 ggggccgccg gtgaacagga tcgaggtctt gttcttgccg gtccagtacc ccgcccaggc
     9181 gtgcgccccg tccagcacct tcttgtgcgt ggcgtcgagc acctcgaacg ccggcaccac
     9241 ctgcccggcc ggcatggcct cgatcttcag agtgccgccc gacatcttgt tcacgcggtc
     9301 ggtgaagtga aggaagttct cgtacagcgt caagctggcg ggccaggtgg cctgcatctt
     9361 gagactggtt tgggcctgcg ccccagcgct gaacgctgcc actgccgcga gcgccacggc
     9421 cgaacggacg tattgcctca tgactcccct cctccaagtt gatttcgcaa ggcgaaaagc
     9481 agggtacacg ttcgaatttg cccggtcaac cggataagcg tcaaaaaaag ggcggccgcg
     9541 gccgcccccc ttgccggcgc tgtgcgcgcc gcgctacttc ttcgccttcg acgcgggttt
     9601 cgccttcgtc tcggggaaat agtagttcgc cgcgaacgaa tacggcggga acatgaagcg
     9661 cttggccggc accaccttgg cggcgtaggc gcgctgcgag tcgagcacct tcttgaagaa
     9721 cgggtccttg gccgattcct cggccgccat cctgtcccag gtcttcatga attcgaccag
     9781 gatctcgggc ggcgtgcgca ggatctgcgt gccgtgcttg gtgcgcatct cgtcgatcgc
     9841 gtcggcgttc tgcttctgcc accggatgcc ccagcgcacg aaggtgtcga aggtcgcggc
     9901 gcgcacagcc tcctgctgct gggcgctcaa gcccttccag acgtcgccgt tcatgatcag
     9961 ctcgagcatc gaattgcttt cgtgcatgcc cggcgtgtag tggtatttcc agacctgcgg
    10021 caggccgagg cgcaggtctt cgacgccgcc cacccactcc gcgcagtcga tgacgccgcg
    10081 ctgcgccgac gggatgatct cgccgcccgg catgttgatc gtggtcatgc ccattttctg
    10141 gtagatctcg gccacgatgc cggtctggcg gcatttcatg cccttgaagt cggccaggtt
    10201 cttgatcggg cgcttgaacc agccgaatgc ctgcgggctc gaaccgccga tcggccagcc
    10261 gacgacgttc agcttcagga cgtcccggta gaactcccag tacaggtcca tgccgccgcc
    10321 ctcgttcatc cagccgagga agtcgaagtg atccatgccg aaggggccgg ccggcgttgc
    10381 ggagaacagc gtcgcggcct tgtgcttgcc gacccagtaa taggcgacgc cgtgcccgcc
    10441 gtcgatcacc ttcttgtgcg tggcatcgag gatctcgaac gccggcacga tctgtcccgc
    10501 ggccatcgcc tcgatcttca cctggcccga ggtcagtttg tccatgcggt cggcaaacat
    10561 cctgaagttg tcctgcagcg tcaggctggc cggccacgtc gactgcatct tcagggtctt
    10621 gacctgggcg tgcgccggaa ctgcaaatcc cagcgccgcc gtgatcacgc tcagcgcgag
    10681 cgtcttgcgg aatgtggttc gcattggatg ccccctcctc atcatgcggt tgtgctgcgg
    10741 gaatccgatg gtaggagctt gaattgggag agtcaatcca gcctggcgac gatcccctcc
    10801 agcgccttcg acggggccgg atagcgcgcc agcaccaggg ggtcgtggcc cgggatcacg
    10861 tgcgccggcg actcggccag gcgctgcgcc ctgtgccagc cctccaccat gtcgccgacg
    10921 ttgtagacga tgggaaacgg ccggctctgc tccatgttgg cgtagtagtg gctggcgtcc
    10981 gaagcgagca ccacccagcc gcggcgcgtc gccacgcgca cgatctgcag ccccatggtg
    11041 tggccgccga cgtgatgcag cgtcacgccg ggcgcgatct caccctcgcc gtcgtgaaag
    11101 cgcacgcgcc ccgcgtacac gcgccgcacc atgccgacca cgtcctccag ctcgtaggcg
    11161 tggcgcatcg cttccaccgc catgcagcgg ccggtggcgt actgcatctc caggtcctgc
    11221 aggtggaagg tggcggcggg aaacaggtcg aagttgccga cgtgatcgta atgcaggtgc
    11281 gtgatggcga cgtccttcac ctgcgccgcg tcgacgccca tccggcgcag gccctcttcc
    11341 accggcagcg tcacgcgccg gccgcgcttg gttgcaatcg cgacgttgaa tcccgtgtcg
    11401 accacgatct cgcgcccctg gccgcggatc agccacacga agtagtccat cggcatcggc
    11461 ccgtcgtgcg ggtcgccgcc gagaaagttc tccttcgccg tgcgcgcatg gtgcgcgtac
    11521 ttgacggcgt atacctcgta gccgctcata cgaccctgat gcgggcgaac ttcctcttgc
    11581 ccacctgcgc caccacagtg gtgccggcgg cgagcttcag cgccttgtcg tcgagccgct
    11641 cgccgtccag cttcacgccg ccctgctcga tgttgcgcgc tgcttccgac acgctcggcg
    11701 ccagccccgc ctgcttcagc gcctgcgtca ccgccaggcc gccggcgggc gagcgcagcg
    11761 tgaactccgg catgtcctcc ggtgcctggc cgtggcggaa gcgctgcgcg aattcctcct
    11821 cggcgcgctc ggccgccgct cgcgagtgga agcgctcgac gatctccctg gcgaagcgca
    11881 ccttgacgtc gcgggggttg gcgcccgctg cgacctcggc gcgccacttg cgcaccgccg
    11941 ccatcggctc gaaggacagc agctcgatgt agcgccacat cagctcgtcg gagatcgaca
    12001 tcagcttgcc gaagatctgc tgcggcggct cggcgatgcc gacgtagttg cccagcgact
    12061 tcgacatctt gttgacgccg tccagtccct cgagcagcgg cgtggtgagg atgcactgcg
    12121 gctcctggcc gtaatccttc tgcagctcgc gtcccaccag caggttgaat ttctggtcgg
    12181 tgccgccgag ctcgacgtcg gacttcatcg ccaccgagtc gtagccctgc atcaggggat
    12241 agagcagctc gtgcaccgcg atcggctggc cggccttgta gcgccgggag aagtcgtcgc
    12301 gctcgaggat gcgcgccacc gtgtagcgcg cggcgagccg gatcatgccc tcggcgccca
    12361 gcttgtccga ccattccgag ttgaacagga tctgcgtgcg ctcggcatcg aggatcttga
    12421 acgcctgcgt gcggtaggtc ttcgcgttct ccagaatctg ctcgcgcgac agcggcggcc
    12481 gcgtctggtt cttgccggtg ggatcgccga tcatgccggt gaagtcgccg atcaggaact
    12541 gcacctggtg ccccagatcc tggaagtggc gcagcttgtt gatcaccacc gtatggccca
    12601 ggtgcaggtc cggcgccgtg gggtcgagcc ccagcttcac gcgcaacggc gtgccgcgcg
    12661 cgagcttgcg cgccagttcc tcctcgacga tcagctcctc ggcgccgcgc ttgatgagcg
    12721 ccagcgcctc ggcgaccggg ggtttcgcgg tttgccgcac cggggatctt tgctagactg
    12781 acgcaggcat ctgaatatca aagaattcta gccgatccat gagccaatac gcgccgctgg
    12841 cgcgcagcct ggccctcctc gccctcgcgc tgtcgggcgt ggtggcggcg ttcgccacca
    12901 tggcgccttc cgccgcgccc ggcccgccgc cgcgcagcgc ggtgaccgaa ccggtcgcgc
    12961 tgcgcgccgg agaagcgctg ctccaggcgc cggcgaccta cctgcgcgag gagcggttcc
    13021 agcgcggcga tacgctcgcc gggctgctcg cgcgcctcgg cgtcgccccg gccgacgcgc
    13081 agaagctgat ccgcgtgccc gccctgcgcc agctgaggcc cggccatgcg gtgagcgtcg
    13141 agatcggcgc cgacggcgaa ctgctgcggc tgagctacct gtcgggccgg gaccggatca
    13201 cggaactgcg gcgcgcaggc gacggtttcc acgcccgcga gctcgccgcg caactgcagg
    13261 cacaggtgct catgcgcgcg ggggtgatcg agagttccct gttcggcgcc gcggatgccg
    13321 cgggcatccc cgatgccgtg gccatccagg ttgcggacat cttcgccggc gacgtcgact
    13381 tccaccgcga actgcgccgc ggcgaccgtt tcgcggtggt ctacgagcag ttcgcgctgg
    13441 acggcgtgcc ggtgcgggcg ggccgcgtgc tggcggccga gttcgtcaac cggggccgca
    13501 cgctgcgcgc cgtgctgtac gcgccggaac gcggcggcag cggctactac gcgcccgacg
    13561 gcagcaacct gcgcaaggca ttcctgcgca cgccgctgga atactcgcgc atcagttcgg
    13621 gcttcggcat gcgccgccac ccgatcca
//
LOCUS       DASRIE010000067        19287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1884681, whole genome shotgun sequence.
ACCESSION   DASRIE010000067 DASRIE010000000
VERSION     DASRIE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884681"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1582)
                     /locus_tag="VFB53_03080"
     CDS             complement(44..1582)
                     /locus_tag="VFB53_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85748.1"
                     /translation="MKYLVYFTAGDHQVYSWARGRLQLEARFPGDDTGVAGFREYLAG
                     RPGALFYALVDLAGEDFHEEQVPYLRGGDREQVVQRRLAQRYRDTRLAAALSLGFVKG
                     ERRNERLLLTSFTNTQQLAPWLDALTESGARLAGVYSVPLLAPALAARLGARGGRAFV
                     VSANSAGLRQCYVEEGKLRFARLERTVDMVPAAFAAFVRSETGRLAQYLSTLRVLPRE
                     GPPVQVLVIAPPGQRAAFEQALVSDARLSFLTVDQDEAARRVGLAGAPAETGAERLYL
                     HLAVRRAPKEQFARREDRRSFHLWQLQRGLVAAGALGFAACALVALAGWYEAAGMRDD
                     TQRLRAEAQAAQQEYQRITSAFPVTQTTTDNLRATVVEFGSIAKRSPAPEGAMLYVSR
                     AMGAFPQIELDSLQWRVERPGERRGERTAKPAAASASGELAQVLELSGRVGAMRRSEY
                     RAITAEVQRFVDALRADPAYRVVRVELPFDITSEGTLSGDIGAAAETGEAPRFVIVLA
                     RSLQ"
     gene            complement(1585..2790)
                     /locus_tag="VFB53_03085"
     CDS             complement(1585..2790)
                     /locus_tag="VFB53_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN85749.1"
                     /translation="MPLYTYKAIDALGKNVVGRTEAVNPFDLEQRLLRMGLDLVYGAP
                     SSQASRLIGGARIKRQDLINFCFHLEQLANAGVPVVEGLVDLRDSVENPRFREVVSGM
                     LESIEGGRNLSQALAEYPEVFGKVFVSLVRSGEQTGRLGDVLKSLTESLKWEDELAAQ
                     TRKLLIYPAFVGGIVLLVTFFLMIYLVPQMTGFIRNMGQEIPLQTRILIHVSDFFVNY
                     WWAVLAAPFIAWFGVKYAAQSNPAVAYTLDRYKIGMPLVGPILKKIVLSRFAASFAMM
                     YASGITVLDAIRSSEELVGNRPVEYALRTAGQQIAEGKNLTVAFQDAGLFPPLVIRML
                     RIGENTGALDTALLNVSYFYNREVKEAIGRLQAMIEPAMTLVLGLILGWVMLSVLGPV
                     YDTISKMKF"
     gene            complement(2823..4532)
                     /locus_tag="VFB53_03090"
     CDS             complement(2823..4532)
                     /locus_tag="VFB53_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN85750.1"
                     /translation="MNSPTNPQAKRHIGQILIDQGILTEDQLRIALLEQTKSKAPVGR
                     LLVQLGFVSEATLRDALSEKLGLQSVDLGHIIVDPAALKMLPRDMARRYRMFPVALDR
                     QSKRFIVALADTNNIVAVDQLRAHLKGDFEVELRLAGDSEVERAIEHYYGHEFSIDGI
                     LREIETGEVDYATIAEGDEYSQPVVRLISALLADAVERAASDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRLKVMAKMNIAETRAPQDGRISATMAGRVVDFRVASLP
                     TTHGENLVLRILDRQKGIVPLDKLGLEDSTLELLKRMIARPEGIILVTGPTGSGKTTT
                     LYSIMNHINSDAINIMTLEDPVEYPMTLIRQTSVSEAAKLDFGNGVRALMRQDPDAIL
                     VGEIRDKDTAEMAFRAAMTGHQVYSTLHTNSAVGAIPRLLDIGIVPDVMSGNIIGVIA
                     QRLVRRLCRTCRQSYDPDVRERRMLGISPTEPAALFRGVGCDQCDYQGYRGRNSILEI
                     LKIDGGLDELIARRATAREILTAARSKGFKTLADDGVRMVRAGLTSLDELMRVVDLTD
                     RMA"
     gene            complement(4582..5871)
                     /locus_tag="VFB53_03095"
     CDS             complement(4582..5871)
                     /locus_tag="VFB53_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN85751.1"
                     /translation="MSLLLEALKKAEKAKEEAQRRAREGEGAEGQPDGEAGAGQRAVV
                     TRAELPQISAPLEILSEDIRAEPARAAAPELSLQAPPAPPPPAAARSDPQAAQRAAAR
                     KVFEAKVREPNPRMPFFLTMGALGAFAVGTVIYFWIQLRPPAPLVNANPVKPSGEVQV
                     AAAAAPGAAQAQPAAPAQPAIQGLPSGVPPARPAEPPAPRARSATPAIPRDPPPPRIR
                     QAASAPQRAPEVPLLATRREMLQVHPRVEAGYAAYQAGRMDAARGEYQQALREEPGNR
                     DALLGLAAVEMQGQRYQAADALYQRVLQADPRDAHAHAGMLALRSQQLDPVAAESRLK
                     TLLADDPRADALHFALGNQYAQQGRWAEAQQAYAQAAAGDPGNPDYAYNLAVSLEHLR
                     QGVPALAQYRRALEQAQKRTASFDAAAARARVQALAR"
     gene            complement(5873..7582)
                     /locus_tag="VFB53_03100"
     CDS             complement(5873..7582)
                     /locus_tag="VFB53_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HZN85752.1"
                     /translation="MNWKTAVSVSLLALALAGCAQPPAKPSDTHLGVEIPPPPAAGSI
                     PPPLQVAPVLPKPQAAARPETYSVVVNNVRVQDLLFALARDARINVDIHPDIAGTVTL
                     NAVDQTLPQLLERIARQVDMRYELDGPNLLVMRDAPVLRTYKVDIVSAARNVKMQSQT
                     STQFGTAGAAGSAAGGSAGQGTGSTATVDVVSNSQLWDTLVQNLRDILQEGDRAQSAA
                     AAPGAKPAAAAAGSGTGGGSVIGNRESGILFVRASARQHEKIQEFLDTVLTNVRRQVL
                     VEATVAEVQLNNEYQRGIDWQRLRTGATATGVAGFGTGQSGVEFRQNSTGTPAAISTT
                     AFVLGGALVSQNLNVAIKLLESFGDVRVLSSPKISVLNNQAALLRVTRDIVYFTITPS
                     TTPVNVTGGGNPNIPPAFTTTPNVAAEGFMMSVLPQINAADTVVLNVRPTIRRRVADA
                     TDPNPALSAATPNLIPIFETREFDSMLRIQSGQTAVLGGLMQDSVENIEDTIPGINRI
                     PLIGWLFSQQRKVSRKTELVIFLRATVIRDASVEGDYSRFRGLLPTENFLNVPNRAAP
                     AAQ"
     gene            complement(7694..9079)
                     /locus_tag="VFB53_03105"
     CDS             complement(7694..9079)
                     /locus_tag="VFB53_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN85753.1"
                     /translation="MALYLEHFGLQEPPFRITPHTDFFFDGADRGATLEALLYAIQHE
                     EGIVKVAGEVGSGKTMLCRVLIERLPKNVETIYLANPSFTRDEILYAVAEELKIEFSR
                     ERVTIALRALQDRLIESYAQGRRTVILIDEAHAMPEETLEQVRLLSNLETHRHKLLQI
                     VLFGQPELDKVLALPSLRQLKDRITHSFRMRPLSQAEAATYLSFRMRAAGYRGPDVFD
                     AAAVAAIARASSGLTRRINILADKALLAAFGAGAHGVSDRHARAAIADSEFTPRWRAP
                     RPALATAAAAAAGVLIGAVGYWLFDPGPAPAAARPAAAPAPVARAAEPPPPPAAPVAA
                     ALLGREQALRLSGYAAGRNPLLQASIAAARERLDTEADARHSIELYLTDNSDPARVER
                     FLMRARLLVSLKDLYIVPLESAGQYRIWALYGVYPDQAAAEQAAKRLPPKYLEAFRPV
                     TRSFAELRRAL"
     gene            complement(9083..10507)
                     /locus_tag="VFB53_03110"
     CDS             complement(9083..10507)
                     /locus_tag="VFB53_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN85754.1"
                     /translation="MATIPQEGRDGRPALLIVDDDPLIADTLAFALGADFEVHACESR
                     AHAIALLRQLPQPPQLALVDLGLPPRPHAPDEGFQLIADLLAHSPAMRIFVLSGQSDA
                     ANARHARALGAAEFVAKPCDPAVLKATLQRALAFRAAEPAGVPADGGLVGDSAALARL
                     RAQIAQSADAPFPVLIEGASGSGKERVAQSLHRLSQRAARPYLALNCAAIAPTLVEPT
                     LFGYVKGAFTGATANKPGYFEDAQDGTLFLDEIGELPLEMQAKLLRVLENGEYQRVGE
                     TQHRVARARVVAATNRDLRQEVRQGRFRSDLYHRLSVLTLAVPALRDMGRDRLLLLEH
                     FRAQYAAQAGVAPFSFDASAERAWLAYPFPGNVRELRNIVIRLITRHAGRTLSVAELE
                     PEFDLESRVAAAPPARDLVAEAQKEIERGGFSLDDTLQAWERAYIEAALRLTRGNMSQ
                     AAKLLGMNRTTLYSRMNTTEEKER"
     gene            10590..11399
                     /locus_tag="VFB53_03115"
     CDS             10590..11399
                     /locus_tag="VFB53_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85755.1"
                     /translation="MADPAIAVIEDLSFAYDRRPVLHGIALSIPRGKVVAVMGQSGCG
                     KTTLLRIIAGALLPTGGRARVLGQVTGELDRDGMYALRRRMGMLFQFGALFTDMTVFD
                     NVAFQMREHTDLPETAVRDLVLLKLEAVGLRGAAQLMPGELSGGMARRVALARAIALD
                     PELILYDEPFTGLDPIALGVVARLVRQLNDSLGATSIIVTHDVAETLPLVDYVYFMAE
                     GRVIAQGTPEAVRRSGVAFVEQFVSGAIDGPVPFHYPAPGYAADLGLAQAA"
     gene            11403..12188
                     /gene="mlaE"
                     /locus_tag="VFB53_03120"
     CDS             11403..12188
                     /gene="mlaE"
                     /locus_tag="VFB53_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HZN85756.1"
                     /translation="MRAALTNLGAAVTAGVWRLGYAARFFGYLLWHSGTAFRRFRLTL
                     REIYFAGVLSLIIILVSGLFVGMVLALQGYETLQRYGSSEALGILVALSLVRELGPVV
                     AGLLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPVARVVAPRFWGGVISMPVLAAL
                     FSAMGIFGGYLVGVVLIGVDEGAFWSQMQAAVDVRNDILNGVIKSVVFGVAVTLIAVF
                     EGYDATPTAEGVSGATTRTVVTSSLAILALDFVLTALMFTGGN"
     gene            12191..12703
                     /gene="mlaD"
                     /locus_tag="VFB53_03125"
     CDS             12191..12703
                     /gene="mlaD"
                     /locus_tag="VFB53_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZN85757.1"
                     /translation="MNRSAIDLWVGVFVALGLGALLFLALKVGNLASFSTAETYGVEA
                     RFENIGGLKVRAPIKSAGVVVGRVAEIRFDNESYEAVALLTLDARYKFPRDTSAKILT
                     SGLLGEQYIGLEAGGDSAMIEAGGRLKITQSAVVLENLISQFLFSKAAEGGEAKGGEA
                     KGNAPKGGKK"
     gene            12700..13401
                     /locus_tag="VFB53_03130"
     CDS             12700..13401
                     /locus_tag="VFB53_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZN85758.1"
                     /translation="MNLRALVVAAALAAGAGCASTSDDPRDPLEGMNRAVYVFNDQVD
                     QIVLAPAARLYKNITPDPLREMVRNFFNNLDDLFIGANNLLQGKLEEAVNDWGRFAFN
                     TVFGLLGVWDVASDMGLEKHNEDFGQTLARWGVGNGAYLVLPLLGPSTLRDTAALPVD
                     WAGDPVAVHRPVDESNAATAFRVVSRRADLLGASQTLEEGALDKYQFHREAFLQRRRS
                     LIYDGSPPRERPPAD"
     gene            13413..14036
                     /locus_tag="VFB53_03135"
     CDS             13413..14036
                     /locus_tag="VFB53_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85759.1"
                     /translation="MKRILAVALLTVGCAAQAQGQMPPDELVKGVMLDIQAIIVKDKD
                     LQAGDRGKTIRLIEDKALPHFNHVGMTALAVGANWRKASPEQKQRLAAEFRTLLVRTY
                     ASSLASFGNQKFDFRPLRMKPSDTDVTVQVRVLQPGAQPVVVEYDLEKTPNGWKCYDV
                     RVAGISLVVNYRTEFANLVREKGIDGLISALAAKNKAVGAQKAGAAK"
     gene            14033..14323
                     /locus_tag="VFB53_03140"
     CDS             14033..14323
                     /locus_tag="VFB53_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN85760.1"
                     /translation="MIRRDGERLIVSGPVTLANVAQLVEEAKAQVAGARAVDLGEVTD
                     LDSSLLAVLIDWQREAKAQGRTLIYARLPEDLRTLAQLYGVAELLPQDKAPS"
     gene            14323..14997
                     /locus_tag="VFB53_03145"
                     /pseudo
     CDS             14323..14997
                     /locus_tag="VFB53_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            15054..15809
                     /locus_tag="VFB53_03150"
     CDS             15054..15809
                     /locus_tag="VFB53_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85761.1"
                     /translation="MTGFNTLLYKELLRFWKVAFQTVAGPVLSALLYLVIFSQALGAH
                     VRVFDGVPYTAFLVPGLVMMSMLQNAFANSSSSLIQSKVTGNLVFVLVTPISHLEFFG
                     AYVLASVARALVVGAGVLAVTFWFVELRLAAPLWALAFALLGSALLAVLGLLAGLWAE
                     KFDQIAAFQNFLVLPLTMLSGVFYSIHSLPPAWQQASHLNPFFYMIDGFRYGFFGAAD
                     FAPAASLAVVAVSFALLSALTLQLLASGYKLRT"
     gene            15812..16051
                     /locus_tag="VFB53_03155"
     CDS             15812..16051
                     /locus_tag="VFB53_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HZN85762.1"
                     /translation="MVTTDSIEQGIRAGLACERVEVVGDGQHFQALVVSQAFAGKSRI
                     QRHQLVYAALGERMREEIHALSMKTLTPEELASGG"
     gene            16044..17297
                     /gene="murA"
                     /locus_tag="VFB53_03160"
     CDS             16044..17297
                     /gene="murA"
                     /locus_tag="VFB53_03160"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZN85763.1"
                     /translation="MDKLLITGGRPLAGEARISGAKNAALPILCAALLTEKPLALDNV
                     PRLMDVRTMGRLLALMGVEVGPQADGRIELRSREIKDPTAGYELVKTMRASVLVLGPL
                     LARCGQAKVSLPGGCAIGARPVDQHVKGLQAMGAEIVVEHGYMNASVKRLRGAHIVMD
                     MVTVTGTENLMMAAALAEGTTLLENAACEPEVVDLAACLKAMGARIEGAGTASIRVEG
                     VRSLSGASHRVMPDRIETGTFLAAVAAAGGKVRLTGAAPDSLGATLEKLREAGAKISA
                     GADSVDIEMSGRPSAVSLTTQPYPGFPTDMQAQLMAMDCIAQGAARIEETIFENRFMH
                     ALELQRLGADIAISGNTATVTGVEKLQGAAVMATDLRASASLVVAGLVAQGETLIDRI
                     YHLDRGYEALEKKLSGLGARITRVK"
     gene            17477..>19287
                     /locus_tag="VFB53_03165"
     CDS             17477..>19287
                     /locus_tag="VFB53_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HZN85764.1"
                     /translation="MRFAILLLSLVAAASSAPAHAFDRSQCKQYGGSSSCWTPVIGPW
                     KYGVCGEWGTGAAGAPFEAAFCEARGGSWVSGACQGLPPVEYRRPTSEGDMRPLALET
                     FSNRYEPLCEGPSGQGFSSWGAVYGSVNCGNGNGATFNSMGYEVGNSATPFAASGKLL
                     LSGVCNANDTVIGFKGSRGRSVGCTSVKGGEDFISTSGSTPALCNLGFRQPIDPKQSC
                     APTCGSDGSGKSTKSVNPQVGNPIDPITGVKQQIELDYAGSGPHPLRFERVYNSRVYT
                     RDGKQWRHNYSAHIENQEFGTVPVVVAHRANGQAFLFKLTGGDYLPDTDIDDRVIRLV
                     DGGGALTGWQYYDASSDANELYDAEGKLLYLINRAGLAHTLEYSTAATPPAVAPAPGY
                     LIRVIDSFGRELNFTHDLFGRLATMLDPASQTYLYESTTLNHFASVTYPDGKSRTYLY
                     NEAGFVGANTYDGRLTGLIDENEVRFGSYFYDFFAMATRSTQPNGVNNTVVNAGSWPS
                     SVNVTDARGTLRTYGYASVNGAYRNTFIRQPAVSGVGTVQTTMTYTGSGNMATRVDFN
                     GNRTNYTSYDTARNLELLRTEGLTAAGASTPQTRTIST"
BASE COUNT         2824 a         6555 c         6937 g         2971 t
ORIGIN      
        1 agcagcggca gcgcgagcag tttcagttcc gcggcggaga agttcattgc aggctccgcg
       61 ccagcacgat gacgaagcgc ggcgcctcgc cggtctcggc ggcggcgccg atgtcgcccg
      121 acagggtgcc ctcggaggtg atgtcgaagg gcagctccac gcgcaccacg cgataggccg
      181 gatcggcgcg cagggcgtcg acgaagcgct gcacttcggc ggtgatggcg cggtactccg
      241 agcggcgcat ggccccgacg cgccccgaga gctcgagcac ctgcgccagc tcgccgctgg
      301 cagatgcagc ggcaggcttg gccgtgcgct cgccccggcg ctcgcccggg cgctccacgc
      361 gccactgcag gctgtcgagc tcgatctgcg ggaaggcgcc catcgcccgc gacacgtaga
      421 gcatcgcgcc ttcgggcgcg gggctgcgct tggcgatgct gccgaactcg accaccgtgg
      481 cgcgcaggtt gtcggtggtg gtctgcgtca ccgggaaagc cgaagtgatg cgctggtact
      541 cctgctgcgc cgcctgcgcc tcggcgcgca gccgctgcgt atcgtcgcgc atgccggccg
      601 cctcgtacca gccggcgagc gcgaccagcg cgcaggcggc gaagcccagc gcgcccgcgg
      661 cgaccaggcc gcgctgcagc tgccacaggt ggaagctgcg ccggtcctcg cggcgcgcaa
      721 actgctcctt cggcgcgcgc cgcaccgcga ggtgcaggta cagccgctcc gccccggtct
      781 cggcgggcgc gcccgccagc ccgacgcggc gcgccgcctc gtcctggtcc acggtgagga
      841 acgacagccg cgcgtcggac accagcgcct gctcgaaggc cgcgcgctgc ccgggcggcg
      901 cgatcaccag cacctgcacc ggcgggccct cgcgcggcag cacgcgcagg gtggagaggt
      961 actgcgcgag gcgcccggtc tccgagcgca cgaaggcggc gaaggccgcc ggcaccatgt
     1021 ccaccgtgcg ttccaggcgc gcgaagcgca gcttgccctc ctcgacgtag cactgccgca
     1081 ggccggcgct gttggcgctc accacgaacg cgcggccgcc gcgcgcgccg agccgcgcgg
     1141 cgagcgcggg cgcgagcagc ggcacggaat acaccccggc cagcctggcg cccgactcgg
     1201 tgagcgcgtc gagccagggc gcgagctgct gggtgttggt gaacgaggtg agcaacagcc
     1261 gctcgttgcg ccgctcgccc ttgacgaagc cgagggacag ggcggcggcg aggcgcgtgt
     1321 cgcggtagcg ctgcgccagg cgccgctgca ccacctgctc gcggtcgccg ccgcgcaggt
     1381 agggcacctg ctcctcgtgg aagtcctcgc ccgcgaggtc caccagggcg tagaacagcg
     1441 cgccggggcg gccggcgagg tactcgcgga acccggccac cccggtgtcg tcgccgggga
     1501 acctggcttc cagctgcagc cgcccgcgcg cccaggagta gacctggtgg tcgccggcgg
     1561 tgaaatagac caggtacttc atcgtcagaa tttcatcttg ctgatggtgt cgtacaccgg
     1621 gccgagcacc gagagcatca cccagccgag gatcaggccc agcaccaggg tcatcgccgg
     1681 ctcgatcatc gcctgcaggc ggccgatggc ttccttcacc tcgcggttgt agaaatagct
     1741 cacgttgagc agcgccgtgt cgagggcgcc ggtattctcg ccgatgcgca gcatgcggat
     1801 caccagcggc gggaacaatc ccgcatcctg gaacgccacg gtcaggttct tgccttcggc
     1861 gatctgctgg cccgcggtgc gcagggcgta ctccaccggg cggttgccga ccagttcctc
     1921 gctcgaacgg atggcgtcga gcacggtgat gcccgaggcg tacatcatgg cgaagctggc
     1981 ggcaaagcgc gacagcacga tcttcttcag gatcgggccc accagcggca tgccgatctt
     2041 gtagcggtcc agcgtgtagg ccaccgcggg attgctctgc gccgcgtact tcacgccgaa
     2101 ccaggcgatg aagggcgcgg cgagcaccgc ccaccagtag ttgacgaaga aatccgacac
     2161 atggatcagg atgcgcgtct gcagcgggat ttcctgcccc atgttgcgga tgaagccggt
     2221 catctgcggc accaggtaga tcatcaggaa aaacgtcacc agcaggacga tgccgccgac
     2281 gaatgccggg tagatgagca gcttcctggt ctgcgcggcg agctcgtctt cccacttcag
     2341 cgactcggtg aggctcttca gcacgtcgcc caggcgcccg gtctgctcgc ccgagcgcac
     2401 caggctgacg aacaccttgc cgaacacctc cggatactcg gcgagcgcct gcgacaggtt
     2461 cctgccgccc tcgatcgatt cgagcatgcc cgacaccact tcgcggaagc gcgggttctc
     2521 gacgctgtcg cgcaggtcga ccagcccctc gaccaccggc acgccggcat tggcgagctg
     2581 ctcgaggtgg aagcagaagt tgatcaggtc ctggcgcttg atgcgcgcgc cgccgatcag
     2641 gcgcgaggcc tggctgctgg gcgcgccgta caccaggtcg aggcccatgc gcagcaggcg
     2701 ctgctccagg tcgaacgggt tgaccgcctc ggtgcgtccg acgacgttct tgcccagcgc
     2761 gtcgatcgcc ttgtaggtgt agagcggcat gcaggcgccg taggctgggc ctgtggcccc
     2821 gcctacgcca tgcggtccgt caggtcgacg acgcgcatca gctcgtcgag gctggtgaga
     2881 ccggcgcgca ccatgcgcac gccgtcgtcg gcgagcgtct tgaacccctt ggagcgcgcc
     2941 gcggtgagga tctcgcgcgc cgtggcgcgg cgcgcgatca gctcgtccag gccgccgtcg
     3001 atcttcagga tctcgaggat cgagttgcgc ccgcggtagc cctggtagtc gcactggtcg
     3061 cagcccaccc cgcggaacag cgccgccggc tcggtcggcg agatgccaag catgcggcgc
     3121 tcgcgcacgt ccgggtcgta ggactggcgg caggtgcggc acaggcgccg caccaggcgc
     3181 tgcgcgatca cgccgatgat gttgcccgac atgacgtcgg gcacgatgcc gatgtcgagc
     3241 aggcgcggga tcgcgcccac cgccgaattg gtgtgcagcg tcgagtacac ctggtggccg
     3301 gtcatcgccg cgcggaacgc catctcggcg gtgtccttgt cgcggatctc gcccaccagg
     3361 atggcgtcgg ggtcctggcg catcagggcg cgcacgccgt tgccgaagtc gagcttggcg
     3421 gcctccgaca ccgaggtctg gcggatcagg gtcatcgggt actcgaccgg atcctccagc
     3481 gtcatgatgt tgatggcgtc ggaattgatg tggttcatga tcgagtacag cgtggtggtc
     3541 ttgccgctgc ccgtgggacc ggtcaccagg atgatgccct cggggcgcgc gatcatgcgc
     3601 ttcaggagct ccagcgtgct gtcttcgagc cccagcttgt cgagcggcac gatgcccttc
     3661 tgccgatcca gaatccgcag caccaggttc tcgccgtgcg tggtgggcag gctggcgacg
     3721 cggaaatcca ccacgcgccc ggccatggtg gccgagatgc ggccatcctg cggcgcgcgc
     3781 gtctcggcga tgttcatctt ggccatcacc ttgaggcgca ccgccatcgc cggccagtag
     3841 gtcttgtgca gggaccggat ctggcgcagc acgccgtcga tgcggtagcg gatgcgcagg
     3901 aaattctgct cgggctcgaa gtggatgtcc gaggcggcgc gctccaccgc gtcggccagc
     3961 agagccgaga tcaggcgcac caccggctgg gagtactcgt cgccctcggc gatggtggcg
     4021 tagtcgacct cgccggtctc gatctcgcgc aggatgccgt cgatggagaa ttcgtggccg
     4081 tagtagtgct cgatcgcgcg ctcgacctcg gaatcgcccg ccaggcgcag ctcgacctcg
     4141 aagtcgccct tcaggtgcgc gcgcagctgg tccacggcga cgatgttgtt ggtgtcggcg
     4201 agggcgacga tgaagcgctt ggattgccgg tcgagcgcca ccgggaacat gcggtagcgt
     4261 cgcgccatgt cgcgcggcag catcttcagc gccgcgggat cgacgatgat gtgtcccagg
     4321 tcgaccgact gcagtccgag cttctcggac agggcgtcgc gcagggtcgc ttccgacacg
     4381 aagccgagct gcaccaggag cctgcccacc ggggccttcg acttggtctg ctccagcagc
     4441 gcgatgcgca gctggtcttc ggtcaggatg ccctggtcga tcaggatctg gccgatgtgg
     4501 cgcttggcct gcggattggt gggcgagttc atccagcctg catgatacag aaagcccgaa
     4561 aaaaccgggc tttaggaact gtcagcgggc gagcgcttgc acccgcgccc gggccgcggc
     4621 ggcgtcgaag ctggcggtgc gcttttgcgc ctgctccagg gcgcgccggt actgcgccag
     4681 cgccggcacg ccctggcgca gatgctccag gctgacggcg aggttgtagg catagtcggg
     4741 gttgccgggg tctccggcgg cggcctgggc gtaggcctgc tgcgcctcgg cccagcggcc
     4801 ctgctgcgcg tactgattgc ccagggcgaa atgcagcgcg tcggcgcgcg gatcgtcggc
     4861 cagcagcgtc ttcaggcggc tttcggccgc caccgggtcg agttgctggc tgcgcagcgc
     4921 gagcatgccg gcgtgcgcgt gcgcatcgcg cggatcggcc tgcagcacgc gctggtacag
     4981 cgcgtcggcc gcctggtagc gctgtccctg catctccacc gccgcgaggc cgagcagggc
     5041 gtcgcggttg cccggctcct cgcgcagcgc ctgctggtac tcgccgcgcg ccgcgtccat
     5101 gcgacccgcc tggtaggccg cgtagccggc ttcgacgcgc gggtggacct gcagcatttc
     5161 gcgccgcgtc gcgagcagcg ggacttccgg cgcgcgctgc ggcgcgctcg ccgcctgccg
     5221 gatgcgcggc gggggcggat cgcgggggat cgcgggggtg gcagagcgtg cgcgcggcgc
     5281 cggcggctcg gcagggcgcg cgggcgggac cccgctcggc aatccctgga tcgccggctg
     5341 cgcgggcgcg gccggctgcg cctgcgcggc gcccggcgcg gccgccgccg caacctgaac
     5401 ttcgccggac ggcttgaccg ggttggcgtt caccagcggg gccggcgggc gcaactggat
     5461 ccagaaatag atgacggttc cgacggcgaa ggcgccgagc gcgcccatgg tgaggaagaa
     5521 cggcatgcgc gggttgggct cgcgcacctt ggcttcgaac accttgcgcg ctgctgcgcg
     5581 ctgcgcggcc tgcgggtcgc tgcgcgcagc ggcgggcggc ggcggcgcgg gcggcgcctg
     5641 cagggaaagt tccggcgccg cggcccgcgc gggctcggcg cggatgtcct cggacaggat
     5701 ctcgagcggc gcgctgatct gcggcagctc ggcgcgcgtc accacggccc tctgcccggc
     5761 gccggcctcg ccatcgggct gcccctcggc gccttcgccc tcgcgcgcgc ggcgctgggc
     5821 ctcttccttg gctttctcgg ccttcttcag cgcctcgagc agcaggctca tgttactgcg
     5881 cggccggggc ggcgcggttc gggacgttga ggaaattctc ggtcggcagc agcccgcgga
     5941 accggctgta gtcgccctcg acgctcgcgt cgcggattac ggtggcgcgc aggaagatca
     6001 ccagctcggt cttgcggctg actttcctct gctgcgagaa cagccagccg atgagcggga
     6061 tgcggttgat gcccgggatc gtgtcctcga tgttctccac cgagtcctgc atcagcccgc
     6121 cgagcaccgc cgtctggccg ctctggatgc gcagcatcga gtcgaattcg cgcgtctcga
     6181 aaatcgggat caggttgggg gtcgccgccg acagcgcggg gttcgggtcg gtcgcgtcgg
     6241 cgacccgccg gcggatcgtc ggccgcacgt tcagcaccac ggtatcggcg gcattgatct
     6301 gcggcagcac gctcatcatg aagccctcgg cggccacgtt cggggtggtg gtgaatgccg
     6361 gcgggatgtt cgggttgccg ccgccggtga cgttgaccgg cgtggtggaa ggcgtgatgg
     6421 tgaagtagac gatgtcgcgc gtgacgcgca gcagcgccgc ctgattgttg agcacgctga
     6481 tcttcgggct ggacagcacg cgcacgtcgc cgaaggattc cagcagcttg atggcgacgt
     6541 tgaggttctg gctgaccaat gcgccgccca gcacgaacgc ggtggtactg atcgctgccg
     6601 gcgtgccggt ggaattctgc cggaactcga cgcccgactg accggtgccg aagccggcga
     6661 cgccggtggc cgtggcgccg gtgcgcaggc gctgccagtc gatgccgcgc tggtactcgt
     6721 tgttgagctg cacctcggcc accgtggcct cgaccagcac ttgccggcgc acgttggtga
     6781 gcaccgtgtc gaggaactcc tggatcttct cgtgctggcg cgcggaggcg cgcacgaaca
     6841 ggatcccgct ttcgcggttg ccgatcaccg agccgccccc ggtgccggag cccgctgccg
     6901 ccgccgcggg cttcgccccc ggcgccgccg ccgcggactg cgcccggtcc ccctcctgca
     6961 ggatgtccct caggttctgc accagcgtgt cccacagctg gctgttggag acgacgtcga
     7021 ccgtggcggt cgagccggtg ccttgtcccg ccgacccgcc ggccgcgctg ccggcggccc
     7081 cggcagtgcc gaactgcgtc gaggtctgcg actgcatctt gacgttgcgc gccgcgctga
     7141 cgatgtccac cttgtaggtg cgcagcacgg gcgcatcgcg catcaccagc aggttcgggc
     7201 cgtcgagctc gtagcgcatg tcgacctgcc tggcgatgcg ctcgagcaat tgcggcagcg
     7261 tctggtcgac ggcgttcagc gtgaccgtgc cggcgatgtc cggatggatg tccacgttga
     7321 tgcgcgcatc gcgcgccagc gcgaacagca ggtcctgcac gcgcacgttg ttcaccacca
     7381 cgctgtaggt ttcgggccgc gccgcggcct ggggcttggg cagcaccggg gcgacctgca
     7441 gcgggggcgg aatgctgccg gcagccggcg gcggcgggat ttcgacgccc aggtgcgtgt
     7501 cggagggttt cgcgggaggt tgcgcgcaac cggccagcgc gagcgcaagc aaggacaccg
     7561 aaacggcggt cttccaattc atcaggtccc ggcctctccc ttggtgcgga agcatagcat
     7621 gggcatgctc taagaaaggc agctaagcgt ctgtcgcggg agagttttca ggattttgca
     7681 aacgccggct ttctcacagc gcgcgccgca gctccgcgaa gctgcgggtc acgggccgga
     7741 aggcctccag gtatttcggc ggcaggcgct tcgccgcctg ctcggccgcc gcctggtccg
     7801 ggtagacgcc gtacagcgcc cagatgcggt actgaccggc gctctccagc ggcacgatgt
     7861 acaggtcctt gagggaaacc agcaggcgcg cgcgcatcag gaagcgctcg acgcgcgccg
     7921 ggtcgctgtt gtccgtgagg tacagctcga tgctgtggcg cgcatcggcc tcggtatcga
     7981 gccgctcgcg cgccgccgcg atgctcgcct gcagcagcgg attgcggccg gcggcgtagc
     8041 ccgagagccg cagcgcctgc tcgcgcccga gcagcgccgc cgccacgggc gcggcagggg
     8101 ggggcggcgg ttccgcggcc cgggcgacgg gggccggcgc ggcagccggg cgcgccgcgg
     8161 cgggcgccgg acccgggtcg aacagccagt agccgaccgc gccgatcagc acgccggcag
     8221 cggctgccgc ggcagtggcg agcgccggcc gcggcgcgcg ccagcgcggg gtgaactccg
     8281 aatcggcgat ggcggcgcgc gcgtgccggt cgctcacccc gtgcgcgccc gcgccgaaag
     8341 cggcgagcag cgccttgtcg gccaggatat tgatgcggcg cgtgaggccg ctggaggccc
     8401 gcgcgatcgc cgccaccgcc gccgcgtcga acacgtccgg cccgcgatag ccggcggcgc
     8461 gcatgcggaa cgacaggtag gtcgcggcct cggcctgcga caggggccgc atgcggaagc
     8521 tgtgcgtgat gcggtccttc agctggcgca gcgagggcag cgcgagcacc ttgtcgagct
     8581 cgggctggcc gaacagcacg atctgcagca gcttgtgccg gtgggtctcc aggttggaca
     8641 gcagccgcac ctgctcgagg gtctcctcgg gcatggcgtg cgcctcgtcg atcaggatca
     8701 cggtgcgccg cccctgcgca taggactcga tcaggcggtc ctgcagggcg cgcagcgcga
     8761 tggtgacgcg ctcgcgcgag aactcgatct tcagctcctc ggcgactgcg tacaggatct
     8821 cgtcgcgcgt gaacgagggg ttggcgaggt agatggtctc gacgttcttc ggcaggcgct
     8881 cgatcagcac ccggcacagc atggtcttgc cgctgccgac ctcgcccgcc accttgacga
     8941 tgccctcctc gtgctggatg gcgtacagca gcgcctcgag cgtcgcgccg cggtcggcgc
     9001 cgtcgaagaa gaagtcggtg tgcggcgtga tgcggaacgg cggttcctgc aggccgaaat
     9061 gctccaggta cagggccacc gctcagcgct ccttttcttc cgtcgtgttc atgcggctat
     9121 agagcgtggt gcggttcatg ccgaggagct tcgccgcctg gctcatgttg ccgcgcgtca
     9181 gccgcagcgc cgcctcgatg taggcgcgct cccaggcctg cagggtgtcg tcgaggctga
     9241 agccgccgcg ctcgatctcc ttttgcgcct cggcgaccag gtcgcgcgcc ggcggcgccg
     9301 cggcgacgcg cgattcgagg tcgaactcgg gctccagttc cgccaccgag agcgtgcgcc
     9361 ccgcgtgccg ggtgatgagc cggatgacga tgttgcgcag ctcgcgcacg ttgccgggga
     9421 acgggtaggc gagccaggcg cgctcggccg atgcgtcgaa gctgaacggc gcgaccccgg
     9481 cctgcgccgc gtactgggcg cggaaatgct ccagcagcag cagccggtcg cgccccatgt
     9541 cgcgcagcgc gggcaccgcc agcgtgagca ccgacagccg gtggtacagg tcggagcgga
     9601 agcgcccctg gcgcacttcc tggcgcaggt cgcggttggt cgccgccacc acgcgcgcgc
     9661 gcgccacgcg gtgctgcgtc tcgccgacgc gctggtattc gccgttctcc agcacgcgca
     9721 ggagcttcgc ctgcatctcc agcggcagct cgccgatctc gtccaggaac agcgtgccgt
     9781 cctgcgcgtc ctcgaagtag ccgggcttgt tggcggtggc gccggtgaag gcgcccttca
     9841 cgtagccgaa cagggtcggc tcgaccagcg tcggcgcgat cgccgcgcag ttgagcgcga
     9901 ggtagggccg cgcggcgcgc tgcgacagcc ggtgcaggct ctgcgccacg cgctccttgc
     9961 cgctgcccga ggcgccttcg atcagcaccg ggaacggcgc gtcggccgac tgcgcgatct
    10021 gcgcgcgcag ccgcgccagc gcggcgctgt cgccgaccag tccgccgtcg gccggcacgc
    10081 cggcgggctc ggccgcccgg aacgccagcg cgcgctgcag ggtggccttg agcaccgccg
    10141 ggtcgcaggg cttggcgacg aactccgcgg cgcccagcgc gcgggcgtgg cgcgcattgg
    10201 cggcgtcgct ctgccccgac agcacgaaga tgcgcatcgc cggcgagtgc gcgagcaggt
    10261 cggcgatcag ctggaagcct tcgtcgggcg cgtgcggcct gggcggcagg ccgaggtcca
    10321 ccagcgcgag ctgcggcggc tgcggcagct ggcgcagcag cgcgatggcg tgcgcgcgcg
    10381 actcgcaggc atggacctcg aagtcggcgc ccagcgcgaa ggcgagcgtg tcggcgatca
    10441 agggatcgtc gtccacgatc agcagtgcag gcctgccgtc gcgcccttcc tgtggaatag
    10501 ttgccatggg gcccctgaca ctatagggga aagcgcggca gccctgcgcg tcgagtaaaa
    10561 tcacgccctt gccgccgtcc cctccccccg tggcagaccc cgccatcgcc gtcatcgagg
    10621 acctgtcgtt cgcctatgac cggcgtccgg tgctgcacgg catcgccctc agcatcccgc
    10681 gcggcaaggt ggtggcggtg atgggccagt ccggatgcgg caagaccacg ctgctgcgca
    10741 tcatcgccgg agcgctcctg cccaccggcg gccgcgcacg ggtgctcggc caggtgaccg
    10801 gcgagctgga ccgcgacggg atgtacgcgt tgcgccggcg catgggcatg ctgttccagt
    10861 tcggcgcgct gttcaccgac atgacggtgt tcgacaacgt cgcgttccag atgcgcgagc
    10921 acaccgacct gcccgagacg gcggtgcgcg acctggtgct gctcaagctc gaggcggtcg
    10981 gcctgcgcgg cgccgcgcag ctgatgccgg gggagctctc cggcggcatg gcacggcgcg
    11041 tggcgctggc gcgcgccatt gcgctcgacc cggagctgat cctgtacgac gagccgttca
    11101 cgggcctcga cccgatcgcg ctcggcgtgg tggcgcgcct ggtgcgccag ctgaacgatt
    11161 cgctcggcgc gacgtccatc atcgtgaccc atgacgtcgc cgagaccctg ccgctggtgg
    11221 attacgtgta tttcatggcc gagggccggg tcatcgccca gggcacgccg gaggcggtgc
    11281 gccgctccgg ggtggccttc gtcgagcagt tcgtcagcgg cgccatcgac ggcccggtgc
    11341 ccttccacta tccggcgccg ggctacgctg ccgacctcgg gctggcgcag gcggcctgag
    11401 ggatgcgcgc cgcgctgacc aatctgggcg ccgcggtcac cgcgggcgtc tggcgcctgg
    11461 gctacgccgc gcgcttcttc ggctacctgc tgtggcactc cggcacggcg ttccggcgct
    11521 ttcgcctgac gctgcgcgag atctacttcg ccggcgtgct ctcgctcatc atcatcctgg
    11581 tgagcgggct gttcgtcggc atggtgctcg cgctgcaggg ctacgagacg ctgcagcgct
    11641 acggctcctc cgaggcgctc ggcatcctgg tcgcgctgtc gctggtgcgc gagctcggcc
    11701 cggtggtggc gggcctgctg ttcgccagcc gggcggggag cgccatcacc gccgagatcg
    11761 gcctgatgaa ggccaccgag cagctctcgg cgatggagat gatggcggtg gacccggtgg
    11821 cgcgcgtggt ggcgccgcgc ttctggggcg gcgtgatctc gatgccggtg ctggcggccc
    11881 tcttttccgc catgggcatc ttcggggggt atctcgtcgg cgtggtgctg atcggcgtgg
    11941 acgagggcgc cttctggtcg cagatgcagg cggcggtgga cgtgcgcaac gacatcctca
    12001 acggcgtcat caagagcgtg gtgttcggcg tcgccgtcac gctgatcgcg gtgttcgagg
    12061 gctacgacgc gacgccgacc gcggagggcg tgtcgggggc gacgacgcgc accgtggtca
    12121 cctcctcgct cgccatcctg gcgctggatt tcgtgctcac cgccctcatg tttaccggag
    12181 gtaactgacg atgaaccgtt ccgccatcga cctgtgggtc ggcgtattcg tggccctcgg
    12241 cctgggcgcg ctgctgttcc tcgcgctcaa ggtcggcaac ctggcgtcgt tctccacggc
    12301 cgagacctac ggcgtggagg cgcgcttcga gaacatcggc ggcctcaagg tgcgcgcccc
    12361 gatcaagagc gccggcgtgg tggtggggcg cgtggccgag atccgcttcg acaacgagag
    12421 ctacgaggcc gtggcgctgc tcacgctcga tgcgcgctac aagttcccgc gcgacacctc
    12481 ggccaagatc ctcacctccg gcctgctcgg cgagcagtac atcggcctgg aggcgggcgg
    12541 cgacagcgcc atgatcgagg ctggcgggcg cctcaagatc acgcagtcgg cggtggtgct
    12601 ggagaacctg atcagccagt tcctgttcag caaggccgcc gagggcggcg aggccaaggg
    12661 cggcgaagcc aagggcaacg cgcccaaggg cggcaagaaa tgaatctgcg tgcgctggtg
    12721 gtcgcggcgg cgctggcggc gggcgcgggc tgcgccagca ccagcgacga cccgcgcgat
    12781 ccgctcgagg ggatgaatcg cgcggtgtac gtgttcaacg accaggtcga ccagatcgtg
    12841 ctcgcgcccg cggcgcgact gtacaagaac atcacgccgg acccgctgcg cgagatggtg
    12901 cgcaatttct tcaacaacct cgacgacctg ttcatcggcg cgaacaattt gttgcaaggc
    12961 aagctcgagg aggcggtcaa cgactggggc cgcttcgcgt tcaatacggt gttcggcctc
    13021 ctgggagtct gggacgtggc gagcgacatg ggactggaga agcacaacga ggatttcggc
    13081 cagacgctgg cgcgctgggg cgtgggcaac ggcgcctacc tggtgctgcc gctcctcggg
    13141 ccgagcacgc tgcgcgacac cgcggcgctg ccggtggact gggcgggcga cccggtggcc
    13201 gtgcaccgcc cggtcgacga gagcaacgcc gccaccgcgt tccgcgtcgt cagccgccgc
    13261 gccgacctgc tgggggcgag ccagacgctc gaggaaggcg cgctcgacaa gtaccagttc
    13321 caccgcgagg cgttcctgca gcgccgccgc agcctgatct acgatggcag cccgccgcgc
    13381 gagcggccgc ccgccgactg aggagaaacg ccatgaaacg aatcctggca gttgccctgc
    13441 tgaccgtcgg ctgcgccgcg caggcgcaag gccagatgcc gcccgacgag ctggtgaagg
    13501 gcgtgatgct cgacatccag gcgatcatcg tcaaggacaa ggacctgcag gcgggcgatc
    13561 gcggcaagac catccgcctg atcgaggaca aggccctgcc gcacttcaac cacgtcggca
    13621 tgacggcgct ggcggtgggc gccaactggc gcaaggcgag cccggagcag aagcagcgcc
    13681 tggcggcgga attccgcacg ctgctggtgc gcacctacgc cagctcgctc gcctccttcg
    13741 gcaaccagaa gttcgatttc cggccgctgc gcatgaaacc gtccgacacc gacgtcacgg
    13801 tgcaggtgcg cgtgctgcag cccggggcgc agccggtggt ggtcgagtac gacctggaga
    13861 agacgccgaa cggctggaaa tgctacgacg tgcgcgtggc cggcatcagc ctggtggtca
    13921 actaccgcac cgagttcgcc aacctggtgc gcgaaaaggg catcgacggc ctgatcagcg
    13981 cacttgcggc gaagaacaag gcggtcggcg cgcagaaagc gggcgccgcg aaatgatccg
    14041 ccgcgacggc gagcgcctga tcgtcagcgg cccggtgacc ctggcgaacg tcgcgcagct
    14101 ggtggaggag gcgaaggcgc aggtcgcggg cgcgcgcgcc gtcgacctgg gcgaagtgac
    14161 ggacctggac tcgtcgctcc ttgccgtgct catcgactgg cagcgcgagg cgaaggcgca
    14221 gggccgcacg ctcatctacg cgcgcctgcc ggaggacctg cgcaccctgg cgcagctcta
    14281 cggcgtggcc gagctgctgc cgcaggacaa agcgccaagc tgatccccgc gatcgaagtc
    14341 cacgaggtcg aaaagcgcta cggcgcgctg cacgcgctcg ccggggtttc gctcgcggtg
    14401 cgccagggcg agttcttcgg gctgctcggc ccgaacggcg ccggcaagac caccctgatc
    14461 agcgtcattg ccggcctcgc ccgcgccagc gcgggccggg tggcggtgat gggcgcggac
    14521 gtggtcgccg actaccggcg cgcacggcgc atgctgggcg tggtgccgca ggagctggtg
    14581 ttcgacccgt tcttcacggt gcgcgagacg ctgcgcctgc agtcgggcta tttcggactg
    14641 cgcggcaacg acgcctggat cgacgaggtg atgcaccacc tcgacctcac cgccaaggcg
    14701 gacgccaaca tgcgcgcgct gtcgggcggc atgaaacggc gggtgctggt ggcgcaggcg
    14761 ctggtgcaca agccgccggt gatcgtgctg gacgagccta ccgccggcgt ggacgtggag
    14821 ctgcgccagg ggctgtggca gttcgtgacg cgcttgaacc gcgacggcca caccatcgtg
    14881 ctcaccacgc actacctgga ggaagccgag gagcattgcg agcgcatcgc catgctcaag
    14941 gcggggcgca tcgtggcgct cgattcgacg cggaacctgc tcgccagctt cgccgggccg
    15001 cacccggcgg acctggagga cgtgttcctg cgcatcatgc ggaagcactg agcgtgaccg
    15061 gcttcaacac gctgctctac aaggagctgc tgcggttctg gaaggtggcc ttccagaccg
    15121 tcgccggccc ggtgctctcc gcgctcctgt acctggtgat cttctcccag gcgctgggcg
    15181 cgcacgtgcg ggtgttcgac ggcgtgccct acacggcgtt cctggtgccc ggcctggtga
    15241 tgatgagcat gctgcagaac gccttcgcca acagctcctc gagcctcatc cagtccaagg
    15301 tgacgggcaa cctggtgttc gtgctggtga cgccgatctc gcacctcgag ttcttcggcg
    15361 cctacgtgct cgcttcggtg gcgcgcgccc tggtggtcgg cgccggcgtg ctcgcggtga
    15421 ccttctggtt cgtcgagctg cgcctcgcgg cgcccctgtg ggcgctcgcc tttgcgctgc
    15481 tgggcagcgc gctgctcgcg gtgctggggc tgctggccgg cctgtgggcg gagaagttcg
    15541 accagatcgc cgcgttccag aacttcctcg tcctgccgct caccatgctc tcgggcgtgt
    15601 tctactccat ccacagcctg ccgccggcct ggcagcaggc ctcgcacctc aaccccttct
    15661 tctacatgat cgacggtttc cgctacggct tcttcggcgc cgccgatttc gcgccggcgg
    15721 cgagcctcgc cgtggtcgcg gtaagcttcg cgctcctgtc ggcgctgacg ctgcagctgc
    15781 tcgcgtccgg ctacaagctc aggacctgac catggtgacc accgacagca tcgagcaggg
    15841 catccgcgca ggactcgcct gcgagcgcgt ggaggtggtc ggcgacggcc agcacttcca
    15901 ggcgctggtg gtgtcgcagg ccttcgccgg caagagccgc atccagcgcc accagctggt
    15961 gtacgccgct ctcggcgagc gcatgcgcga ggaaatccac gccctgtcga tgaagacgct
    16021 gacccccgag gagctggcct ccggtggata agctgctgat caccggcggg cggccgctcg
    16081 cgggcgaggc gcgcatctcg ggcgcgaaga acgccgcgct gccgatcctg tgcgctgcgc
    16141 tgctcacgga aaagccgctc gcgctggaca acgtgccgcg gctgatggac gtgcgcacca
    16201 tgggcaggct cctcgcgctg atgggcgtgg aagtggggcc gcaggcggat ggccgcatcg
    16261 aactgcgctc gcgcgagatc aaggacccga cggcgggcta cgagctggtg aagaccatgc
    16321 gcgcctcggt gctggtgctg gggccgctgc tcgcgcgctg cggccaggcg aaggtgtcgc
    16381 tgcccggggg ctgcgccatc ggcgcgcggc cggtggacca gcacgtgaag ggcctgcagg
    16441 cgatgggcgc ggagatcgtg gtggagcacg gctacatgaa tgccagcgtg aagcggctgc
    16501 gcggcgcgca catcgtcatg gacatggtca ccgtgaccgg caccgagaac ctgatgatgg
    16561 cggcggcgct cgccgagggc acgacgctgc tggagaacgc ggcctgcgag ccggaggtgg
    16621 tggacctcgc cgcttgcctc aaggcgatgg gcgcgaggat cgaaggcgcc gggacggcaa
    16681 gcatccgcgt cgagggcgtg cgctcgctct cgggcgccag ccaccgcgtg atgccggacc
    16741 gcatcgagac ggggaccttt ctcgctgcgg tcgccgcggc cggggggaag gtcaggctga
    16801 cgggcgctgc cccggattcg ctgggcgcga ccctggaaaa gctgcgcgag gcgggcgcga
    16861 agatttccgc cggcgcggac agcgtcgaca ttgagatgag cgggcgtcct tcggcggtga
    16921 gcctcaccac gcagccctat cccgggtttc ccaccgacat gcaggcgcag ctgatggcga
    16981 tggattgcat cgcgcagggc gcggcgcgca tcgaggaaac catcttcgag aatcgcttca
    17041 tgcatgcgct cgagctgcag cgcctgggcg ccgacatcgc gatcagcggc aacaccgcga
    17101 cggtgaccgg ggtggagaag ctgcaggggg ccgccgtgat ggcgaccgac ctgcgcgcct
    17161 cggcgagcct ggtggtggcg ggcctggtgg cgcagggcga gacgctgatc gaccgcatct
    17221 accatctgga ccgcgggtac gaagcgctgg agaagaagct ctcagggctg ggtgcgagga
    17281 ttacgcgggt gaagtaagtc cagcatttct cgcggattgc gcgcttccgc tcggacagcg
    17341 cgcaaatctg ctttcgagat atctcgaatt ttggatgagc cggccggacc acggcgttag
    17401 cgtttttctc tgaccccgtg tagcgaattc cttgtcacca tgatcgcgaa atttcacggt
    17461 ggcatgggag gctccgatga gattcgcgat cctgctgctt tccctggttg cggcggcgtc
    17521 ctcggcgcct gctcacgcct tcgaccgctc gcaatgcaag cagtacggcg gctcgagcag
    17581 ttgctggacg cccgtcatcg gcccctggaa gtacggtgtg tgcggcgaat gggggaccgg
    17641 ggccgccggg gcgccgttcg aggccgcgtt ctgcgaggcg cgaggcggca gctgggtctc
    17701 aggcgcctgc caggggctgc cgccggtcga ataccggcgc ccgacctccg agggcgacat
    17761 gcggcccctt gccttggaga ccttcagcaa taggtacgaa ccgctgtgcg aagggcccag
    17821 cgggcagggc ttttcctcct ggggcgccgt gtacggcagc gtgaactgcg gcaacggcaa
    17881 cggcgcgacg ttcaattcga tgggctacga agtcggcaat tcggcgacgc cgtttgcggc
    17941 atcgggcaag ttgctcctgt cgggggtgtg caacgcgaac gacacggtga ttggctttaa
    18001 aggctcgcgc gggcgctcgg tgggctgcac ttccgtgaag ggaggagaag acttcatttc
    18061 gacgagcggg agcaccccgg cgttgtgcaa cctgggcttc cgccagccaa tcgaccccaa
    18121 gcagagctgc gcgccaacct gcgggtcgga tggatcgggg aagtccacaa aatcggtcaa
    18181 cccccaggtg ggcaacccga tcgacccgat caccggggtg aagcagcaaa tcgagctgga
    18241 ctacgcgggc agcggcccgc acccgcttcg cttcgagcgc gtctacaaca gccgcgtcta
    18301 cacgcgcgac ggcaagcaat ggcggcacaa ctactcggcg cacatcgaga accaggagtt
    18361 cggcacggtg ccggtggtgg tcgcgcaccg cgcgaacggg caggcattcc tcttcaagtt
    18421 gaccggcggc gactacctcc ccgacacgga catcgacgac cgggtgatcc gcctcgttga
    18481 cggaggcggc gcgctcaccg gctggcagta ctacgatgct tcgagcgacg cgaacgagct
    18541 ctacgacgcc gaaggcaagc tcctctacct catcaaccgc gcgggcctgg cccacacgct
    18601 cgaatacagc accgcggcaa cgcccccggc agttgcgccc gcacccgggt acctcatccg
    18661 ggtgatcgac tccttcggca gggagctcaa cttcacccac gacctcttcg ggcggctcgc
    18721 gacgatgctg gaccccgcca gtcagacgta tctctacgag agcaccacgc tgaatcattt
    18781 cgcgagcgtc acctaccccg acggcaagag caggacctat ctctacaacg aagcggggtt
    18841 cgttggagcg aacacctacg acgggcgcct gacgggcttg atcgacgaga acgaggtgcg
    18901 cttcgggtcc tatttctacg atttcttcgc catggccacg aggagcaccc agcccaatgg
    18961 ggtgaacaac accgtggtga acgccgggag ctggccctcc agcgtgaacg tcaccgacgc
    19021 ccgcggcacg ctcaggacct acggctacgc gtcggtgaac ggcgcctacc gcaacacctt
    19081 catccgccag ccggcggtct ccggggtcgg taccgtgcag accacgatga cctacacggg
    19141 cagcggcaac atggccacgc gggtggattt caacggtaac cgcaccaact acacctccta
    19201 cgacacggcg aggaatctgg agctcttgcg caccgagggc ctgactgcag caggtgcgag
    19261 cacgccccag accaggacca tctcgac
//
LOCUS       DASRIE010000068         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_334512, whole genome shotgun sequence.
ACCESSION   DASRIE010000068 DASRIE010000000
VERSION     DASRIE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334512"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFB53_03170"
     CDS             complement(<1..190)
                     /locus_tag="VFB53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85765.1"
                     /translation="MNPPRALRAFRHRSFRIFFFGQGISQFGTWLQLIATSWLVYRLT
                     GSTFWLGLAGFALQVPFLV"
     gene            complement(187..2967)
                     /locus_tag="VFB53_03175"
     CDS             complement(187..2967)
                     /locus_tag="VFB53_03175"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZN85766.1"
                     /translation="MALDKSFDPRAVEGRWYERWESAGWFAHRGDAKPAYAIQLPPPN
                     VTGTLHMGHAFQQTLMDALIRYHRMRGFNTNWVAGTDHAGIATQIVVERQLEAEGKTR
                     HDLGREKFVERVWQWKQQSGSTITRQMRRLGASANWEYADTEGQKAGYFTMDAKMSRA
                     VVEVFVRLYEEGLIYRGKRLVNWDPVLGTAVSDLEVDAEETNGTMWRIVYPFVDGPQD
                     STDREGRPVKLRGMTIATTRPETMLADGAVAVHPEDPRYRHLVGKRVILPLCDRTIPV
                     VADTMVDREFGTGCVKITGAHDFNDYGAALRHKLPMIVIFTLDAKINENGPRQFQGMD
                     RFAARKAVVAQLEREKFLAGADPHRMMVPKSGRTGAVVEPMLTDQWFVKMDGLARDGL
                     EVVARGEVKFFPEHWAKTYNEWLANIQDWCISRQIWWGHQIPAWYDDQGNVHVARSEK
                     EAAAKAGGRKLQRDPDVLDTWFSSQLVPFSALGWPEKTPDLERFLPSDVLVTGNDIIF
                     FWVARMIMATRHFTGKVPFRHVYINAIVRDAEGEKMSKSRGNTLDPLDLIDGITLEDL
                     LQKSTQGLLKGEHRERIEKYVRAHYPAGIPAFGADAVRFTFASLASFAITLNFDLGRC
                     EGYRNFCNKLWNAARFVLMNAEGKDCGQDDRLPVELSAWDRWIVSSLQRAEAEAAQGF
                     DEYRFDNAAGAIYRFIWDEYCDWYLEVAKEQLAKGSAAQQRATRRTLVRVLEAALRLS
                     HPIIPFITEELWQSVAPLAGKAGETIMLAPYPKSQPEKIDEAAEREIALAKEVVNAGR
                     NLRSEAKVPPKDRIPFYLVGQPSASIVAAATALLRASELRIVAELPRSDSPVAVAGPH
                     RLMPHIEVDPVAERARIAKEVARLQGEIAKARTQLSNSAFVDRAPAAVVAQMRARLAA
                     FETTLEKLSAKP"
     gene            complement(2970..3269)
                     /locus_tag="VFB53_03180"
     CDS             complement(2970..3269)
                     /locus_tag="VFB53_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85767.1"
                     /translation="MSDGDPLVQRVDALLKRHAETAGAADDVPVLTEIVGAEASAAEA
                     LALELERAVLARLENALDGTLATLREALRVAVADAVARELEARGFAARPPEPPGG"
     gene            complement(3266..3700)
                     /locus_tag="VFB53_03185"
     CDS             complement(3266..3700)
                     /locus_tag="VFB53_03185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZN85768.1"
                     /translation="MTGIDFYFNAGDRLQVACRLAGKALTQGKRTLIYAPDAETAQRV
                     DRLLWTWPATGFVPHCALHDPLAARTPVLIGDEPAAAAGCDVLLNLGAATPPAFERFE
                     RLLEVVALDDEGRRAARERYRFYRDRGYKIADHDLAGAGAGA"
     gene            complement(3697..5199)
                     /locus_tag="VFB53_03190"
     CDS             complement(3697..5199)
                     /locus_tag="VFB53_03190"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZN85769.1"
                     /translation="MEFSIRTLAPELAKCGCLVLGVTQAGAGAPALTRAAQAADRAAR
                     GRLREVLAQGDLSGKAGSTLLLHKVAGLAAERVLLVGLGESKAFGETAFRDAVRGAAA
                     ALKALGARDATVYLADAKVGRRPAAWNLRHVVLGLRDAFYRFDQLKTEKQAPAPALAS
                     VTLAQQSGPVTQQAQAALREAAATGDGVDLARTLGNLPPNICTPAYLAGEAGKLARQY
                     KLGLEVLERRDMERLGMGALLAVTRGSHQPPKLIVLRYAGGAKKDRPVVLVGKGITFD
                     TGGISLKPAGEMDEMKFDMSGAGSVLGTLRALAGMKAPVNVVGVIPSCENMPGGAAAR
                     PGDVVKTMSGQTVEILNTDAEGRLILCDALAYAARFQPDAVVDIATLTGACVIALGHV
                     ATGLFANDEKLAGQLSAAGDDAWDRVWRMPLWEDYQEQLRSNFADMANIGGRPAGSVT
                     AACFLSRFARKLRWAHLDIAGTAWKSGRDKGSTGRPVPLLVRFLLDRAGK"
     gene            5236..>5511
                     /locus_tag="VFB53_03195"
     CDS             5236..>5511
                     /locus_tag="VFB53_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZN85770.1"
                     /translation="MIFLRALLREFGQLATAVFLSLFLIALTTRLVRLLGQAASGKIP
                     SDAVVAFLGFFALNALPVLLSLTLFVTVLLTLTRSYRDSEMVIWFGAG"
BASE COUNT          760 a         1938 c         1910 g          903 t
ORIGIN      
        1 gcacgaggaa gggaacctgc agcgcaaaac cggccaggcc gagccagaag gtcgagccgg
       61 tgagccggta gaccagccag ctggtggcga tgagctgcag ccaggtgccg aactgggaga
      121 tgccctggcc gaagaagaag atgcggaagc tgcggtggcg gaatgcgcgc agcgcgcgcg
      181 gcgggttcac ggcttggcgg agagtttttc cagcgtggtt tcgaacgccg caaggcgcgc
      241 ccgcatctgc gccaccactg cagccggtgc gcgatcgacg aatgcggagt tgctgagctg
      301 ggtgcgcgcc ttggcgatct ctccctgcag gcgcgcgacc tccttggcga tgcgcgcgcg
      361 ctcggcgacg gggtcgactt cgatatgggg catcaagcgg tgcggtccgg ccacggcgac
      421 cggggaatcg gatctcggca gttcggcgac gatgcgcagt tccgaggcgc gcagcagggc
      481 ggtggcggcc gcgacaatgg acgcgctcgg ctgtccgacc aagtagaacg gaatccgatc
      541 tttcggcggc accttggcct cgctgcgcag gttgcgcccg gcgttgacca cttccttggc
      601 cagcgcgatc tcgcgctcgg cggcttcgtc gatcttctcg ggctggctct tcgggtaggg
      661 cgccagcatg atcgtttcgc ccgccttgcc ggcgagcggc gcgacgctct gccatagctc
      721 ctcggtgatg aacggaatga tcgggtgcga caggcgcagc gcggcttcca gcacccgcac
      781 cagcgtgcgg cgcgtggcgc gctgttgtgc tgcgctgccc ttcgccagct gttccttggc
      841 gacctccagg taccagtcgc aatactcgtc ccagatgaag cggtagatcg cccccgcggc
      901 attgtcgaag cggtactcgt cgaagccctg cgccgcctcc gcctcggcgc gctgcaggct
      961 gctgacgatc cagcggtccc aggcggaaag ctccaccggc agcctgtcgt cctgcccgca
     1021 gtccttgccc tcggcattca tcagcacgaa gcgcgccgcg ttccacagct tgttgcagaa
     1081 attgcggtag ccctcgcagc gcccgaggtc gaaattcagc gtgatggcga agctggcgag
     1141 cgaggcgaag gtgaagcgca ccgcgtcggc gccgaaggcg ggaatgcccg cggggtagtg
     1201 cgcgcgcacg tacttctcga tgcgctcgcg gtgctcgccc ttcagcagcc cctgcgtgga
     1261 cttctgcagc aggtcctcga gcgtgatgcc gtcgatcagg tccagcggat cgagcgtgtt
     1321 gcccctggac ttggacatct tctcgccttc ggcgtcgcgc acgatggcgt tgatgtacac
     1381 gtggcggaag ggcaccttgc cggtgaaatg gcgcgtcgcc atgatcatgc gcgccaccca
     1441 gaagaagatg atgtcgttgc cggtgacgag gacgtcggag ggcaggaacc tttcgaggtc
     1501 gggtgtcttc tccggccatc cgagcgcgct gaacggcacc agctgggaag agaaccacgt
     1561 gtcgaggacg tccgggtcgc gctgcaattt cctgccgcct gcctttgcgg cggcttcctt
     1621 ctcgctgcgg gcgacatgga cgttgccctg gtcgtcatac cacgccggga tctggtgtcc
     1681 ccaccagatc tgccgcgaga tgcaccagtc ctggatgttg gcgagccact cgttgtaggt
     1741 cttcgcccag tgctcgggaa agaacttcac ctcgccgcgc gccaccacct cgagcccgtc
     1801 cctggccagg ccgtccatct tcacgaacca ctggtcggtg agcatcggct ccaccaccgc
     1861 gccggtgcgg ccggacttgg gcaccatcat ccggtgcggg tcggcgccgg cgaggaactt
     1921 ctcgcgctcg agctgcgcca ccaccgcctt gcgggcggcg aagcggtcca tgccctggaa
     1981 ctgcctcggc ccgttctcgt tgatcttcgc gtcgagcgtg aagatgacga tcatcggcag
     2041 cttgtggcgc agcgcggcgc catagtcgtt gaagtcgtgc gcgccggtga tcttcacgca
     2101 gccggtgccg aactcgcggt cgaccatcgt gtccgcgacc accgggatgg tgcgatcgca
     2161 cagcggcagg atcacccgct tgccgaccag atgccggtag cgcgggtcct ccgggtgcac
     2221 cgccaccgcg ccgtcggcca gcatggtctc cgggcgggtg gtcgcgatcg tcatgccgcg
     2281 cagcttgacc ggcctgcctt ccctgtcggt cgaatcctgc gggccgtcga cgaagggata
     2341 gacgatgcgc cacatcgtgc cgttcgtttc ctcggcgtcg acctcgaggt cggaaacggc
     2401 ggtgccgagc accgggtccc agttgaccag ccgcttgccg cgatagatca gcccctcctc
     2461 atagaggcgc acgaagacct cgacgacggc gcgcgacatc ttcgcgtcca tcgtgaagta
     2521 gccggccttc tgcccctccg tatcggcgta ctcccagttc gcggatgcgc cgaggcggcg
     2581 catctggcgc gtgatcgtgg agcccgactg ctgcttccac tgccagacgc gctcgacgaa
     2641 cttctcccgt ccgaggtcgt ggcgcgtctt gccctcggcc tcgagctggc gctcgaccac
     2701 gatctgcgtg gcgatgccgg cgtggtcggt gcccgccacc cagttggtgt tgaagccgcg
     2761 catgcggtgg tagcggatga gcgcgtccat cagcgtctgc tggaaggcgt gccccatgtg
     2821 cagcgtgccg gtcacgttcg gcggcggcag ctggatggca taagcgggct tggcgtcgcc
     2881 gcggtgggcg aaccagcccg ccgattccca gcgctcgtac cagcggcctt cgaccgcgcg
     2941 cggatcgaag ctcttgtcca gcgccatgct cagccgcccg gcggctcggg cggcctggcg
     3001 gcgaagccgc gcgcctcgag ctcgcgggcc acggcgtcgg cgaccgccac gcgcagcgcc
     3061 tcgcgcagcg tcgccagcgt gccgtcgagc gcgttttcca gccgcgcgag gaccgcgcgc
     3121 tccagttcga gggccagcgc ctcggcggcg gaggcctcgg cgcccacgat ctcggtgagc
     3181 acgggcacgt cgtcggccgc gccggcggtc tccgcgtggc gcttgagcag cgcgtcgacc
     3241 cgctgcacca ggggatcgcc gtcgctcatg cgccggcacc ggcaccggcc aggtcgtggt
     3301 cggcgatctt gtagccgcgg tcgcggtaga agcggtagcg ctcgcgcgcc gcgcggcggc
     3361 cctcgtcgtc cagcgccacc acttccagca ggcgctcgaa tcgctcgaag gcgggcggcg
     3421 tcgcggcgcc gagattgagc agcacgtcgc agcccgcggc ggcggcgggt tcgtcgccga
     3481 tcagcaccgg ggtccgcgcg gcgagcggat cgtgcagggc gcaatgcggc acgaacccgg
     3541 tcgccggcca ggtccacagc aggcggtcca cgcgctgcgc ggtctcggcg tccggcgcgt
     3601 agatcagcgt gcgcttgccc tgcgtcaggg ccttgcccgc caggcggcag gcgacctgca
     3661 gccggtcccc ggcgttgaag tagaagtcga tgccggtcat ttgcccgcgc ggtccagcag
     3721 gaacctcacc agcagcggca ccggccggcc ggtggagccc ttgtcgcggc cgcttttcca
     3781 ggccgtgccc gcgatgtcca ggtgggccca ccgcagcttg cgcgcaaagc gcgacaggaa
     3841 gcaggccgcg gtgacgctgc cggccgggcg gccgccgata ttcgccatgt cggcgaaatt
     3901 ggagcgcagc tgctcctggt agtcttccca cagcggcatg cgccagacgc ggtcccaggc
     3961 gtcgtcgccc gccgcgctca actgcccggc gagtttttcg tcgttggcga acaggccggt
     4021 ggccacgtgg ccgagggcga tcacgcaggc gcccgtcaac gtggcgatgt cgaccaccgc
     4081 gtccggctgg aaacgcgcgg cataggcgag cgcgtcgcac aggatcaggc ggccctcggc
     4141 gtcggtattg aggatctcga ccgtctgccc cgacatcgtc ttcaccacgt cgccgggccg
     4201 cgccgccgcg ccgccgggca tgttctcgca gctggggatc acgccgacca cgttgaccgg
     4261 cgccttcatg cccgcgagcg cgcgcagggt gcccagcacg ctgccggcgc cggacatgtc
     4321 gaatttcatc tcgtccatct cgcccgccgg cttgagcgag ataccgccgg tatcgaaggt
     4381 gatgcccttg ccgaccagca ccacgggccg gtccttcttc gcgcccccgg cgtagcgcag
     4441 cacgatcagc ttgggcggct ggtgcgagcc gcgcgtgacc gcgagcaggg cgcccatgcc
     4501 gaggcgctcc atgtcgcgcc gctcgagcac ctcgaggccg agcttgtact ggcgcgcgag
     4561 tttgccggcc tcgccggcca ggtaggcggg cgtgcagatg ttgggcggca gattgcccag
     4621 cgtgcgcgcc agatcgacgc cgtcgcccgt cgccgccgcc tcgcgcagcg cggcctgcgc
     4681 ctgctgcgtc acgggcccgc tctgctgcgc gagcgtgacc gacgccagcg caggcgccgg
     4741 cgcctgcttc tcggtcttca gctgatcgaa gcggtagaac gcgtcgcgca gtcccagcac
     4801 gacgtggcgc aggttccagg cggcggggcg gcggcccacc ttggcgtcgg cgaggtacac
     4861 ggtcgcatcc cgcgccccga gcgccttgag cgcggcggcg gcaccgcgca ccgcgtccct
     4921 gaacgccgtt tcgccgaatg ccttcgactc gcccaggccg accagcagga cgcgttccgc
     4981 cgccaggccc gccaccttgt gcagcagcag cgtggagccc gccttgccgc tcaggtcgcc
     5041 ctgcgccagt acttcgcgca ggcgcccgcg cgccgcgcga tcggcggctt gcgccgcgcg
     5101 cgtcaatgcc ggcgcgccgg cgcccgcctg ggtgactccg agcaccaggc aaccgcactt
     5161 ggcaagctct ggcgccagcg tccgtatgct aaattccacc ggattctccc ttgaaatgag
     5221 cggtttaagt atcgcatgat ctttctccgc gcgctgctgc gcgagttcgg gcaactggcg
     5281 accgcggtgt tcctgtcgct gttcctgatc gcgctcacca cgcgcctggt ccggctgctc
     5341 ggccaggcgg cgagcggcaa gataccttcc gatgcggtgg tggcgttcct cggcttcttc
     5401 gcgctcaatg cgctgccggt gctgctgtcg ctgacgctgt tcgtcaccgt cctgctcacc
     5461 ctgacgcgca gctaccgcga ctcggagatg gtgatctggt tcggcgccgg c
//
LOCUS       DASRIE010000069         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_845553, whole genome shotgun sequence.
ACCESSION   DASRIE010000069 DASRIE010000000
VERSION     DASRIE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845553"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFB53_03200"
     CDS             <1..1006
                     /locus_tag="VFB53_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN85771.1"
                     /translation="RTCLELRKGGFVPDVICAHPGWGEALYLKEVFPEAKLLNFYEFY
                     YGARGSDLGFDPEFPTSFDDRFKVPTRNATHLLSLASGDWGVSPTHWQREQFPPVWRA
                     MISVVHDGIETRLVKPDPRAVLKLEKQRLELSRKDEVITYVSRNLEPYRGFHIFMRAL
                     PEILKRRPRATVLIIGGDEVSYGRKPTKGKTWREVMMAEVGGALDMSRVHFTGKVPYG
                     QFLQLLQISSVHVYLTVPFVLSWSLLEAMAAGCLIVASGTQPVREAMDDGVNGLLVDF
                     LSPAQVAARVEEALVNQDRLQPLRERARQTVVERYDLLSVCLPQQLALIEWLAARKAS
                     "
     gene            1026..1736
                     /locus_tag="VFB53_03205"
     CDS             1026..1736
                     /locus_tag="VFB53_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZN85772.1"
                     /translation="MLDFLGIGAQKAGTTWLYEALAQHPQVRFPAGKEVHFWDERRDQ
                     GLEWYQALFAGVAPGTRAGEVTPAYAILPPAVLGEIRALNPALRAIYVLRNPIERAWS
                     AALMALARAGMALDEAPDRWFVDQFRDRGSLQRGDYETCLRNWRAAFDPAQILVLRYE
                     MLREEPLALLEACCRHLGVEPAFYRGRAASLRARVHAGPQAPLRPSLLPVLREIYRPR
                     IASLAAYLGHDLGAWLAT"
     gene            1721..2575
                     /locus_tag="VFB53_03210"
     CDS             1721..2575
                     /locus_tag="VFB53_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85773.1"
                     /translation="MARNVRRSTPASPRRSGAPPAAAWRFDARGRDAQARADIAVIVT
                     TVLRPSLEAALASVFRQDFAGHLQLLVGVDRATGSVQPLLDTLAARPARISALVLYPG
                     YSTSARHGGLHKASDGGALRTVLSYLANAPLLAYLDDDNEWLPNHLSSLAAAIAGFDW
                     AYSLRWFVDQRTGHDLCVDTWDSAGPGKGERGGTLGGFSDVNCLMIDKTRAEAVLPLW
                     SIPLAKWRATADRQVFRRLCMDHPAAWSGLATVRYAIRPSFYLWPKIRRHLAAMPRAP
                     SLLPLDGA"
     gene            complement(2572..>3488)
                     /locus_tag="VFB53_03215"
     CDS             complement(2572..>3488)
                     /locus_tag="VFB53_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HZN85774.1"
                     /translation="ALLELGSGFNAEFTGRENVVMNAAILGLEGDEIRRRMPEIEAFA
                     GIGEFIDQPVKTYSSGMLMRLAFAVSVSVEPEILIVDEALSVGDIVFQFRCLERLSRL
                     VGSGTTLLFVSHDIGTVRAFCSRAVYLEAGRVKAIGSTEDITEAYISDMHEARRRDRG
                     AAFAVPARAQGGPGVLSARFADAAGAAYSHGDAIEIEVEVEAAGLACPALSALIQDSR
                     LVAVAGTVCTLQRGDGAGRAKVRFAARALFLPGHYFVTLRLEERPGDGSFILIDKQIG
                     ILSFEVVHGAQRTFLGLVDIGMSARQLP"
BASE COUNT          488 a         1273 c         1226 g          501 t
ORIGIN      
        1 gcgcacctgc ctcgagctgc gcaagggcgg cttcgtgccg gacgtgatct gcgcgcaccc
       61 cggctggggc gaagcgctct atctcaagga agtgttcccc gaagcgaagc tgctcaactt
      121 ctacgagttc tactacggcg cgcgcggctc ggacctgggc ttcgacccgg aattccccac
      181 ctccttcgac gaccgcttca aggtgccgac gcgcaacgcc acgcacctcc tcagcctcgc
      241 ctcgggggac tggggcgtga gcccgaccca ctggcagcgc gagcagtttc cgccggtgtg
      301 gcgcgcgatg atctcggtgg tgcacgacgg catcgagacc cggctggtga agcccgatcc
      361 gcgcgcggtg ctcaagctcg agaagcagcg gctcgagctc tcgcgcaaag acgaagtgat
      421 cacttacgtt tcccgcaatc tcgagccgta ccgcggcttc cacatcttca tgcgcgccct
      481 gcccgagatc ctgaagcggc ggccgcgcgc cacggtgctg atcatcggcg gcgacgaggt
      541 gagctacggg cgcaagccga ccaagggcaa gacctggcgc gaggtgatga tggccgaggt
      601 cggcggcgcg ctcgacatgt cgcgcgtgca cttcaccggc aaagtgccct acggccagtt
      661 cctccagctg ctgcagatct cgtcggtgca cgtgtacctg acggtgccgt tcgtgctgtc
      721 gtggtcgctg ctcgaggcga tggcggcggg ctgcctgatc gtcgcctcgg gcacgcagcc
      781 ggtgcgcgag gcgatggacg acggcgtcaa tggcctgctg gtcgacttcc tgtcgccggc
      841 gcaggtcgcg gcccgggtcg aggaggcgct ggtgaaccag gaccggctgc agccgctgcg
      901 cgagcgcgcg cggcaaacgg tggtcgagcg ctacgacctc ctgtcggtgt gcctgccgca
      961 gcagctcgcg ctgatcgagt ggctcgcggc gcgcaaggcg agttgaaacc ggtccggttc
     1021 ttcgcatgct cgacttcctc ggcatcggcg cgcagaaagc cggcaccacg tggctctacg
     1081 aggcgctcgc ccagcacccg caggtgcgct tcccggcggg caaggaggtg catttctggg
     1141 atgagcggcg cgaccagggg ctcgagtggt accaggcgct gttcgccggc gtggcgcccg
     1201 gcacgcgcgc cggtgaggtc acgccggcct acgcgatcct gccgccggcg gtgctcggcg
     1261 agatccgcgc gctgaacccc gcgctgcgcg cgatctacgt gctgcgcaac ccgatcgagc
     1321 gcgcgtggtc cgcggcgctg atggcgctgg cgcgcgccgg gatggcgctc gacgaggccc
     1381 ccgaccggtg gttcgtcgat cagttccgcg accgcggctc gctgcagcgc ggcgactacg
     1441 agacctgcct gcgcaactgg cgcgcggcat tcgacccggc gcagatcctg gtgctgcgct
     1501 acgagatgct gcgcgaggaa ccgctcgcgt tgctcgaggc gtgctgccgg cacctcgggg
     1561 tcgagccggc gttctaccgc ggccgcgccg cgtcgctgcg cgcacgggtc cacgccgggc
     1621 cgcaggcgcc gctgcggccg agcctgctgc cggtgctgcg cgagatctac cggccgcgca
     1681 tcgcgagcct cgccgcgtac ctcggccacg acctcggcgc atggctcgca acgtaagaag
     1741 aagcacgccg gcgagtccgc ggcggtcggg ggcgccccct gcggccgcct ggcgcttcga
     1801 cgcgcgcggg cgcgatgcgc aggcgcgcgc cgacatcgcg gtgatcgtga ccacggtgct
     1861 gcggccaagc ctcgaggccg cgctggcctc ggtgttccgc caggatttcg ccggccacct
     1921 gcagctgctg gtcggcgtcg accgggccac cggcagcgtg cagccgctgc tcgacacgct
     1981 ggccgcgcgc ccggcgagga tcagcgccct ggtgctctat ccgggctact ccacctcggc
     2041 gcgccacggc gggctgcaca aggcctccga cggcggcgcg ctgcgcaccg tgctcagcta
     2101 cctcgccaac gcgccgctgc tcgcctacct cgacgacgac aacgagtggc tgccgaacca
     2161 cctgagcagc ctcgccgccg cgatcgcggg cttcgactgg gcgtactcgc tgcgctggtt
     2221 cgtggaccag cgcaccggcc acgacctgtg cgtcgacacc tgggactcgg ccggccccgg
     2281 gaagggggag cgcggcggca cgctcggcgg gttctcggac gtgaattgcc tgatgatcga
     2341 caagacgcgc gccgaggccg tgctgccgct gtggtcgatc ccgctggcga aatggcgggc
     2401 gaccgcggac cggcaggtgt tccgccgcct gtgcatggac catccggcgg catggagcgg
     2461 gctcgccacg gtgcgctacg ccatccggcc gagcttctac ctgtggccga agatccgccg
     2521 gcacctcgcc gccatgccgc gcgcgcccag cctgctgccg ctcgacggcg cctaggggag
     2581 ctggcgcgcg ctcatcccga tgtcgaccag cccgaggaag gtgcgctgcg cgccgtgcac
     2641 cacctcgaag gacaggatcc cgatctgctt gtcgatcagg atgaacgagc cgtcgcccgg
     2701 gcgctcttcc agccgcaggg tgacgaaata atgcccgggc aggaatagcg cgcgcgccgc
     2761 gaagcgcacc ttggcgcggc cggcgccgtc gccacgctgc agcgtgcaca ccgtgccggc
     2821 gacggcaacc aggcgcgagt cctggatcag cgcgctcagc gccgggcacg ccagcccggc
     2881 cgcctcgacc tcgacctcga tctcgatcgc gtcgccgtgc gaataggcgg cgcccgcggc
     2941 gtcggcgaag cgcgcactca gcacgcccgg cccgccctgc gcgcgcgccg gcacggcaaa
     3001 cgccgcgccg cggtcgcgcc ggcgcgcttc gtgcatgtcg ctgatgtacg cctcggtgat
     3061 gtcctccgtc gagccgatcg ccttgacgcg cccggcctcg aggtacacgg cgcggctgca
     3121 gaaggcccgc accgtgccga tgtcgtgcga cacgaacagc agcgtcgtcc ccgagccgac
     3181 caggcggctc aggcgctcga ggcagcggaa ctggaacacg atatcgccca ccgacagcgc
     3241 ctcgtcgacg atcaggatct cgggctcgac gctcaccgac accgcgaacg cgagccgcat
     3301 cagcatgccg ctcgagtagg tcttcaccgg ctggtcgatg aactcgccga tgccggcgaa
     3361 cgcctcgatc tcgggcatgc gccgccggat ctcgtcgccc tcgagtccga ggatcgcggc
     3421 gttcatcacc acgttctcgc gcccggtgaa ctcggcgttg aagccgctgc cgagctcgag
     3481 cagcgccg
//
LOCUS       DASRIE010000070        36553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3470419, whole genome shotgun sequence.
ACCESSION   DASRIE010000070 DASRIE010000000
VERSION     DASRIE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 36553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3470419"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFB53_03220"
     CDS             <1..680
                     /locus_tag="VFB53_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZN85775.1"
                     /translation="FIDAPFLFRDLAHWNKVLDQDLLKPIADEIAQKADVMLIGYAGG
                     GTRNIFINKPMGSLAEIKGLKVRVQGAPIWSKTFQAVGMAPTVIAYNEVYNAIQNGVI
                     AAGENEAAGVEQMKFYEVAPHLSMTQHAITIRPLCFSGKTFKKLDKALQAAVLKAGKE
                     AGAYGRQIESSEDEAKLAKMEKEGKLKRVPFAGRDQMKKLVDPVMAEYAKEIGADQIL
                     AKINAIK"
     gene            685..1212
                     /locus_tag="VFB53_03225"
     CDS             685..1212
                     /locus_tag="VFB53_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZN85776.1"
                     /translation="MTEANAAAPERRRFFMLRKLTHWYGTLLSWLLVASVAVLVFPVS
                     LQIFSRYTALIPSYIWTEEMARFFFIWSIMLGAMVGIREGTHFVVDLWPPMNARAQAA
                     VRLLSSVFVLAFAAVFLWWGIDFTRFAFNRISELAELPLWVIHVAWPLAGATWLLFQG
                     EHAWHDLQLLRRGKA"
     gene            1209..2528
                     /locus_tag="VFB53_03230"
     CDS             1209..2528
                     /locus_tag="VFB53_03230"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZN85777.1"
                     /translation="MTGATLLDPATASLIMFGAFFLLLALRVPVAFALGLACLPVFLI
                     EDRLTPQNLVQETFNAYNSFILLAVPFFLLTANLMNVGGITTRLVRLSRDLVGHFPGG
                     LAQVNVVLSIFFAGISGSSTADAASQSKIFIEAQRKEGYHDSFSVAITAVSAVLAVII
                     PPSILMIVWGGLLTVSIGGLFLAGIIPGLLIGLVQMATVHVYAKLRNYPTYPRATLVE
                     TIKAIGVAIPALMTPVIIIGGKIFGWFTATESACIAVLYAAALTMLVYREMDWKGLHG
                     ALLETGRLTGVALFCVGTASAFGWLLAYYQIPKALLAGVAEWGMGPMTTGFFIAGVFL
                     VVGCFLDAIPAIVIVGTVLEPLARSVGLDPIHFAMIGIVSLAFGLVTPPYGLCLMIAA
                     HVAGMRVVDTLKDVFIMLVPMLAVLALVIMWPELVLFLPKLFPPGTL"
     gene            2549..3334
                     /locus_tag="VFB53_03235"
     CDS             2549..3334
                     /locus_tag="VFB53_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN85778.1"
                     /translation="MSLNTATTREQWGLAQAIEGCARHGIRGIAPWRDQLAAMGVKEA
                     VRAIKSNGLTVTGLCRGGFFTAPDWKDDNRRAIEEAHALGAQCLVLVVGGLIPGSRDL
                     PGSREKVKEGIAAILPEARKAGVPLAIEPLHPMQAADRACVNTLEQALDICDALGDGV
                     GVAADVYHLWWDPKLESQIARAGKQRLLAYHICDWLVPTRDLLNDRGMMGDGVIDLPG
                     IRGMVERAGYAGFHEVEIFSKLDWWRRDPDEVLATCKDRHRRT"
     gene            3337..4386
                     /locus_tag="VFB53_03240"
     CDS             3337..4386
                     /locus_tag="VFB53_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN85779.1"
                     /translation="MRGAVFLGNRKIEIRNFPDPVPGPGEVVIQMKASGMCGSDLKFY
                     RPPPGEAQKALGLGGNAEPFIGGHEPCGVIAARGAGVSEREAPIGQRVMNHHYAGCGV
                     CGHCRVGWSQLCRAGIVVYGVTGNGGHAEYLKAPARTMVPLPEELSFAEGAAVSCGTG
                     TAYGALRRMHLAGGSTLAVFGQGPVGISATLLGARMGARVIAVEANAERLALARTYGA
                     DALVDSSKVDAEQALRDLTRGEGVDLALDCTGVAAARLAAVRSAKTWGTVCFVGEGGE
                     VTLDVSRDMLRKQLTLIGSWTFSAMGMGECARFVADNRIPLEKIFTHRWKLDQVQEAY
                     RVFDAQSSGKGVLEF"
     gene            complement(4383..5330)
                     /locus_tag="VFB53_03245"
     CDS             complement(4383..5330)
                     /locus_tag="VFB53_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN85780.1"
                     /translation="MSATWQPSPRYPDPSIVAMDDSFNRYRLPLAKVERLATGVTRWA
                     EGPVWFGDQKCLVWSDVPGDCMWRWDEATGAVGAWRRPSGYANGNTRDRQGRLVTCEH
                     GGRRVSRTEPDGRIVALAERYEGKRLNSPNDVVCKSDGSLWFTDPSFGILGWYEGAKA
                     EAELPTNVYRIDPSGALSVVAEGINQPNGLAFSPDESLLYVVESRSVPRKILAYDVKG
                     GKSLANRRVLIDAGPKGTPDGFRVDVDGNLWCGWGMGEAGLDGVHVFNPEGRPIGRID
                     LPERCANVCFGGAHRNRLFMAASTSIYALYVNTQGLPYF"
     gene            complement(5333..5800)
                     /locus_tag="VFB53_03250"
     CDS             complement(5333..5800)
                     /locus_tag="VFB53_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZN85781.1"
                     /translation="MGGWQTFGGVLALLAAGAAAAQGIHRWTDERGRVHYGNEPPRDA
                     RAMQVPERVSTYAGPVDVRRVPGVVKAPAAAAAQPLVMYATSWCPVCAKARAYFRKHG
                     IAYEEHDVEKSPEAKAEFTRLGGKGVPLIVHGRAAMSGFSENGFEALLARSGR"
     gene            5857..6837
                     /locus_tag="VFB53_03255"
     CDS             5857..6837
                     /locus_tag="VFB53_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85782.1"
                     /translation="MARILLTHSPETLERYYGARALAGLQGLGEVRLHRGGAPLEGEA
                     LIAAAAGCELIVSYRQSSAPAALFERLPALAAFLRCAVDIRNIDVAAASRAGVLVTQA
                     SAGFVPAVSELVTGFLVDLSRGISRAAAEYRAGRVPAASTGRELRGSTLGIIGYGAIG
                     RELARVARALGMRVLVHDPYVKGIEQTPRDDLLAQSDYVVPLAVATPETENLMDDAAF
                     AKMKRGAFFINVSRGNLVDEAALARALDSGRIAGCALDVGRAPDQMPTPALAARADVI
                     ATPHTAGLTTPAIEHQSLETVAQAGEILQGRAPKGAVNAEHWTRKSLLTP"
     gene            6834..7664
                     /locus_tag="VFB53_03260"
     CDS             6834..7664
                     /locus_tag="VFB53_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN85783.1"
                     /translation="MKTILITGAAGDVGTHLRRELAGKYRIRASDLRPLKKVAQETFM
                     RADIAKFADALKITRGVDAIVHLGGYSVEGPWEGILSANIVGCYNVFEAARRNGVKRI
                     LFPTSNHATGFFRRDQTIDHRVYIKPDSRYGVSKVFGEALGSLYADKYGMQVFCMRIG
                     NVNPLPIDKRRLAIWFSPRDLAQLVTIGIEHPEIRFEIVYGVSGNQRSWYDNANAFRL
                     GYRPQDDSEVYAKEVLAREKPGGHPVAEAHQGGVFCVAEEVPNPAPLPRLRKRAARTS
                     "
     gene            complement(7636..8529)
                     /locus_tag="VFB53_03265"
     CDS             complement(7636..8529)
                     /locus_tag="VFB53_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN85784.1"
                     /translation="MHHLRSPALLLMLAMLCWAGNWVVGRAIHEQVPPFTLNFWRWAG
                     SLLLILPVAWPALRRQWPLLLRHWKWVLPMAAIASAIFQSMVYLGLQSTTALNGALII
                     ALVPVLVVAIAAAILGERIGARQGLGILVSLAGAVVVIVRGELSVLRALAFNPGDLWI
                     LAAVPIWALYTVLLKRWPAGLERMSFLAAMALVGVAVQSPFFAWEWAAGRHMVVTPAS
                     LAAIAYTAVFASFLAFVFYNAALQRTTATVAGPFHHLHPAFTALLGIAFLGERIGWHH
                     AAGVGCIVLGLYLTTSARRAS"
     gene            complement(8532..9191)
                     /locus_tag="VFB53_03270"
     CDS             complement(8532..9191)
                     /locus_tag="VFB53_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85785.1"
                     /translation="MFIGHAAVAFAAKPLAPRTSLALLLAAAFWVDMVWPLFLLLGIE
                     RVKIDPGNTAFTPLDFLHYPWTHSLAAALGWSVLFGLACLRSGRRVALILGLLVFSHW
                     VLDAVTHRPDLPLWPGSEAMIGLGLWNSVPATIAVECAMFAAGVWLYARSAPSQDRVG
                     SWAFWGLIAFLAVAYLGNVFGPPPPGETAIAIAGLAGGILFTAWAWWADRHRGAKTPK
                     M"
     gene            complement(9195..9605)
                     /locus_tag="VFB53_03275"
     CDS             complement(9195..9605)
                     /locus_tag="VFB53_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HZN85786.1"
                     /translation="MLAVLTGCASVPMGSDAEDLAAKGFMPRQGKANIYVYRHETMGA
                     AVKMPVSLNGRIAGLTASKTYFLFEVDPGSHRLASHTENVAELTVNASAGRNYYVWQE
                     VKMGLMQARSNLQLMDETAGRAGVMECKRAQASF"
     gene            complement(9716..11782)
                     /gene="fusA"
                     /locus_tag="VFB53_03280"
     CDS             complement(9716..11782)
                     /gene="fusA"
                     /locus_tag="VFB53_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN85787.1"
                     /translation="MARHLIKHLRTLALVGQAGSGKTSLVEALLARAGAIAAAGSVER
                     GTTVCDYTPQEKQLQHSLKLAVASFAVNGGEQPTFVHLLDTPGYPDFLGHALPALAAV
                     ETAAIVINAQNGIEMMTGRFMQWAAKRGLDRLVVVNKIDAENVDLEGLLGRIQQAFGK
                     ECLPLNLPAAKAAKVSDCFFAPAGEADFSSVAEAHQRLVDQVVEVDEQLMASYLEKGE
                     VSPEELHEPLERAMREGHLVPVVFCSAKSGAGVQELLEVIVKLMPNPSEGNPPAYLSV
                     PVDGGAPAEIQALPDPGKHALAHVFKIEIDPYIGRLAVFRVHQGRITPGTQLYLGEGR
                     KPVKAGHLYLLRGKSQTEVAEAVPGDICAIAKVDEIQFDQILHDSPGDAHIRARPLEL
                     PVPVFGLAVQPKKRGDEQKVSDVLHKLVAEDPCFRIEHNAQANETVMRGLGEMHLRTT
                     LDKIANQYKVELDTRPPSIPYRETIASRAEGHSRHKKQTGGAGQFGEVFLKVEPLPRG
                     KGFEFVDMVKGGAIPNQFIPAVEKGVRGVLGTGFLAGYPIQDLRVIVYDGKSHPVDSK
                     EVAFVSAGRKAFLDALAKARAIVLEPIVNVSIVAPEHKMGDITGELSGHRGHIRGSDA
                     PRPGTVQIAAQAPLAELEQFPARLKSLTAGHGSYTLEFSHYEPAPPQLQQKLVAAHRP
                     AAHADD"
     gene            complement(11830..12903)
                     /locus_tag="VFB53_03285"
     CDS             complement(11830..12903)
                     /locus_tag="VFB53_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZN85788.1"
                     /translation="MNARIAGVLLVLLAALGGGALLLQQQRAAERPAGAGALGQPLLK
                     NLKAADIAAIVLREPGASLSLRNQAGRWTIAERGGFPADLDKVRALVLKALELKVGQS
                     EPIGGQDRARLKLDGSGTALEFLGADGKPLARLAVGSKFFKREPANPALAIGDGRFVQ
                     LPEDPQTAIVVSDPLTLASTRSADWIERTGFAAEKVMAMETALPGGEKWRIERARDDA
                     GWQLSPSRAGEKLDAMRANSASYSLNSVALADVAAPGLKSADTGLDRPFATIVARTFD
                     GLTYTVRLGKPAGENYYAAVAVAGEPKATGADAAERQKTLAARLPAERALAAHTLLIA
                     KSKFDDILKKRAELLEQKGAGRK"
     gene            complement(12900..14768)
                     /locus_tag="VFB53_03290"
     CDS             complement(12900..14768)
                     /locus_tag="VFB53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HZN85789.1"
                     /translation="MKKHEALLYSAVGLAALFLLLVAFNYLAAGAALRADLTEGGLYT
                     LSDGTKKLLRGLQAPVKVKLYLTAGEAMPVQLRSFARRVEDLAREFKSVAGDKLVIEK
                     YDPRPDSEEEDAAQLDGIEPQQLASGEQFYLGLAVSQLDRKQSIPVVAPQRERLLEYD
                     LVRAIARAGIAERPKIGVMSGLPVLGERFNPMTRQSSEPWVLASELQRDFDVVQVNMT
                     TDAIDKDLGVLLLVHPRDISELAEFALDQFVLRGGRLIAFVDPYAYFDQQQPPMPGMP
                     AFASSSTLPRLFKAWGVEMDPAKVVSDVVFASGGGQRYTPTVLSLNRTAFSRDDVVAS
                     QIETLLYAFGGAFAVKPAEGLAASELVRSSPNSMLVDSASATVAGDAATRSFQPGGKP
                     LLLALRLAGKFKTAFPAGRPVDKNAKPKPGAADAAPLKVSARDGAVVLVADADMLQDG
                     AAVDVQEVFGRRIVVPSNGNLAFAQGLVEQLASDDSLLSLKSRAAAFRPLTVVREMEA
                     EAQAQYFGKIKELEDELQKTTENMQKLQKSPGAGAKAAQILSPEQQAELESFRKKLAE
                     SRKALKELRKNLRQDSERLVFLTKLVNIALMPLAVALAGLLVALLRRRRQARLAAA"
     gene            complement(14765..15499)
                     /locus_tag="VFB53_03295"
     CDS             complement(14765..15499)
                     /locus_tag="VFB53_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85790.1"
                     /translation="MNPWHAIRTIAKRELASYFISPVAYVFLVIFLLLTGFLTFTAGA
                     FFERGEASLGAFFSWHPWVYLMLVPAAGMRLWAEERRAGTLELLLTMPVTAWQAILGK
                     FLASWAFLAIALALTFPVTVTVNVLGDPDNGAIAAGYLGSLLLAGAYLAVTCLTSALT
                     RNQVIAFILAVVVCLFLILAGFNPVTDLLVRWASPAFVDTVAAFSVITHFEGFQKGVI
                     DSRDLLFFLSVIGFALFATGVALRNR"
     gene            complement(15496..16212)
                     /locus_tag="VFB53_03300"
     CDS             complement(15496..16212)
                     /locus_tag="VFB53_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN85791.1"
                     /translation="MIRIENLVKAFGAKRAVDGVSFAVERGEVLGFLGPNGAGKSTTM
                     RMITGYFPPTSGRVTVGGHDVVEAPLAAKRLIGYLPEAAPSYPDMTVQGFLAFAAEMR
                     GLRGAARRHAVGRAVERCFLASVLHQTIDTLSKGYKHRACLAQALIHDPEVLILDEPT
                     DGLDPNQKHEIRNLIRELGRTKAIIFSTHILEEVDAVCSRAIIIDRGRIVASGTPDEL
                     GRPLDEVFRRITLPDTVKKS"
     gene            16312..17253
                     /locus_tag="VFB53_03305"
     CDS             16312..17253
                     /locus_tag="VFB53_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN85792.1"
                     /translation="MLKLAKFLVLMTFAAAAAAQPSLKMMIPANPGGGWDQTGRNLAA
                     AMQSAKLVSGVQFENKGGAAGAIGLAQFVNAAKGDPNAVMIGGMVMVGGTYLQNSPVT
                     LAQVTPIARLTSEWEVIVVPAASPHKTMADLVAAFKADPGKVSWGGGSAGGTDHILAG
                     LIAQAVGVEAKRVNYVPFKGGGEAVAAIIGGHVTAGVSGIGEFAEHVKSGKMRALAVS
                     APSKTDGIPSLKEQGIDVVLGNWRSIFAAPGITPAQRDALVKLVRDATNTPAWKGTLE
                     KMGWSGEFLAGEEFKAFLEADQKRIAGIIDSLGIKKK"
     gene            17261..17755
                     /locus_tag="VFB53_03310"
     CDS             17261..17755
                     /locus_tag="VFB53_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN85793.1"
                     /translation="MRRSRAEIALSIGVLGLGAGAAWVTARLPGEGGYAGIGPNFIPA
                     VVSGGLLLMGAWLLWEALAGGWRNLAAPEADAEHPFNAAAFGWVSAGLFAHMALIGWA
                     GFVLAGAALFAGVARGFGSPRPARDAALGFALSLAVFLFFVKLLNVNLPAGWLQPLLG
                     GAGI"
     gene            17757..19241
                     /locus_tag="VFB53_03315"
     CDS             17757..19241
                     /locus_tag="VFB53_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN85794.1"
                     /translation="METFNALLHGFSVALTPMNLMWGFIGVTVGTAVGVLPGVGPALT
                     VAMLLPLTVKLDPTGALIMFAGIYYGAMFGGSTTTILLNTPGESASIATALEGNRMAK
                     RGRAGPALATAAIGSFVAGSIATVLLTLLAPLVVELALKFGPAEYFALMVFAFTTVAA
                     VLGSSTARGLASLFLGLLFGLVGIDGQTGAARFAFGVPELLDGIDVVVLAVGLFAVGE
                     ALYMAAYTSRLPGTLEKLSGSLMMSRDDWKRSWPAWLRATCIGFPFGTIPAGGAEIPT
                     FLSYSVERKLSKHPEEFGHGAIEGVAGPEAANNASATGVLVPLLTLGIPTSATAAILL
                     SAFQNYGIQPGPLLFQSQADLVWGLIASLYVGNVLLLVLNLPLVGIWVKVLAIPRPML
                     YGGILVFATLGAYSLHQSWVDLATLYVFGLLGFAMRRFDIPVAPAVIGLILGPLAETQ
                     FRRALSISQGDATVFLTQPISASFLAVTAALVIVPWLVRRYGRR"
     gene            complement(19226..19660)
                     /locus_tag="VFB53_03320"
     CDS             complement(19226..19660)
                     /locus_tag="VFB53_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZN85795.1"
                     /translation="MRNAIAVLGLALLAGCGSLGDPKANVAEDKVVYHVNDSAQQAMA
                     ALRNIGNHLEVNPKAKIVVVTHAQGVDFLFKDARDRNGNPYQVAVEGLKERGVQFDVC
                     EITLRNRKLGRGQFIEEARFVPSGVAEITRLQQREGYAYLRP"
     gene            complement(19674..20234)
                     /locus_tag="VFB53_03325"
     CDS             complement(19674..20234)
                     /locus_tag="VFB53_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN85796.1"
                     /translation="MRPELWLCAALLAGAAQAEEKLISGPGSTLTENKCRICHELKHI
                     QRAQLSPGEWADNLKNMKERGTPMDEAEMAVIQRYLSTYYNRAQAAPPPSPDTLAGGG
                     DDPVQKLLNAHACVGCHAPDKRVVGPSFREVAAKYAGDAGAAARLAAKVRAGGVGNWG
                     QVPMPPNPGIPDADLQAMVGWVLGNK"
     gene            complement(20224..21438)
                     /locus_tag="VFB53_03330"
     CDS             complement(20224..21438)
                     /locus_tag="VFB53_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZN85797.1"
                     /translation="MRDHQGSLTRRGFLRAAAGAGALATTAGELLAQAAPATAPGTMA
                     NEDVKAGKAPTMEWHSERPLTGSVPAHEHDFEVTPSQRMFVRNNHTTPLIDINGHRLA
                     LKGLVDKELSFSVFELAKEFKTVSIHGMLECAGSGRSGYQPRASGTPWLPTGGMGCPK
                     WGGVRLRDLLQAAGLKPGAAHVAGQGGDPGAVATAAPVIRSVPMKKAMDEDTLVAWAM
                     NDAPLPFVHGYPLRLVVPGWVGSASTKWLHTLTVLDAPFKGTFMDSSYRIPRFPVEPG
                     TKMPPDAVSTEAWPVKSMITFPAPNARFKPGEAIAVRGKAWVGEGSVDRVEVSVDEGK
                     TWRPAQLAKGSGRHAWRSFTYEHKAEAPGYVTFLARAWDDRGNAQPALPAWNPLGYFW
                     NAWHRVGVVVAA"
     gene            21600..22064
                     /gene="soxY"
                     /locus_tag="VFB53_03335"
     CDS             21600..22064
                     /gene="soxY"
                     /locus_tag="VFB53_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZN85798.1"
                     /translation="MQGTRRNLLRLGWSMGLLAAFGAALVRPGRALAAWNKGAFEAKT
                     VAEGMQALGISGAVESKDLVIRAPDIAENGAVVPIDITSGIPGTTAISVFIEKNPFPY
                     AGSFDFSQGAVPFVHLRAKIGESTPVRVVAAAGGRYYSAVKEVKVTIGGCGG"
     gene            22078..22398
                     /gene="soxZ"
                     /locus_tag="VFB53_03340"
     CDS             22078..22398
                     /gene="soxZ"
                     /locus_tag="VFB53_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HZN85799.1"
                     /translation="MAEPMKIRATLQGDVADVRVLMAHPMETGQRKDAAGKIVPLHFI
                     QSIGATLNGKPVFSADISQAISRNPVFAFKVKGARAGDTIAITWTDNKGETRTDQAVV
                     RAAP"
     gene            22412..23215
                     /gene="soxA"
                     /locus_tag="VFB53_03345"
     CDS             22412..23215
                     /gene="soxA"
                     /locus_tag="VFB53_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HZN85800.1"
                     /translation="MRRALVLALAAAPWLHAAAQAEDPVAAEFDKFRAVLEQDNPAEL
                     FEVKGEELWTKPRGPKNASLAACDLGLGPGVVKGAYAQLPRYFADVDRVMDAEARIVH
                     CMITLQGYSYAELAKQPFSSLTRTPDPVSIATYVAAQSAGMKIAAPQAHPKERAAYAA
                     GQEMFFYRAGPYDFSCASCHGVDDKRIRTQPLPNFTRPAQAVRAVQGWPGYRMTGGVM
                     HTLQWRMNDCFRQQRFPEPGYASEMVSALLTYLTVNANGHEYRGPGIKR"
     gene            23226..23828
                     /gene="soxX"
                     /locus_tag="VFB53_03350"
     CDS             23226..23828
                     /gene="soxX"
                     /locus_tag="VFB53_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HZN85801.1"
                     /translation="MRPAAIAVLAAAALAAACAGLQSDPRADARALMQRDFHAKGSAG
                     MDRLKEDPVQSACNAHRDNPPQALAEKLQQEQMAAIKWPADGKLLGDWKSGEKIAQDG
                     RGMAWTDKGGVGGSCYNCHRIGPATTSFGTIGPSLHHFGKLRGYGAEMQRYAYGRIYN
                     AKAYNLCTQMPRFGHAGALSEQQMKDLTALLMDPASPVNQ"
     gene            23866..24501
                     /locus_tag="VFB53_03355"
     CDS             23866..24501
                     /locus_tag="VFB53_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN85802.1"
                     /translation="MRYAWLAAVAVPLALAGAPAAQASDRGPYVAVMMTGSEPSGPKP
                     TASTDLDGFSGVGLSAAVGYRWLPLRVELEYQGNTAYWIGSLFSGSGDDRIQMKTLML
                     NAIVEAQLNGWLGVFFGAGFGQADVRADFVTCLQLQGCLAPAESHASGSASARQTQLG
                     VTVGPFHQHQVVIGFRWLKSGRLGLADTAGRPFEEDRAKAKMSFVGWRGSF"
     gene            complement(24472..26130)
                     /gene="soxB"
                     /locus_tag="VFB53_03360"
     CDS             complement(24472..26130)
                     /gene="soxB"
                     /locus_tag="VFB53_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HZN85803.1"
                     /translation="MPHNNSRREFLRWLSFAAAAGASLRPGLGAAQAADELYQPPPYG
                     KVSLLHLTDVHAQLLPLYFREPAGAAFLERQGIAAGSAAAYAHSRVDFERAARRYGKV
                     GGYAHLATLVKRLRASRPHALLLDGGDSWQGSATALWTGGADMIGAQKLLGVDLMTAH
                     WEFTYGAKRVREAVDRELAPIEFLAQNVKTADFEDPVFKPYSLRSLNGVALAVIGQAF
                     PYTPIANPRHLVAEWTFGIQEPRLQQLVDEVRAKGAQAVVLLSHNGLQVDLKLAARVR
                     GIDAILGGHTHDGIAKPVRVGRTLVTNAGSHGKFLGLLDLEVQGGAVTGYRYRLLPVF
                     ANLLPADAAMASYIDKVRAPYRAQLEEKLAATEGLLYRRGTYNGSWDELLLEALLKEK
                     DAEIAFSPGFRWGGTLLPGDAITFEDLMDQTAITYPYATLNELTGAQIKAILEDVADN
                     TFNPDPYLQQGGDMVRVGGLSYACRPRAGVGARISDLRLGGRPLEAGRRYKVAGWAPV
                     AEGSSGEPVWELAARYLRARRTIAPLKPRAPRLLASEAPAPADK"
     gene            26129..28858
                     /locus_tag="VFB53_03365"
                     /pseudo
     CDS             26129..28858
                     /locus_tag="VFB53_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            28858..29478
                     /locus_tag="VFB53_03370"
     CDS             28858..29478
                     /locus_tag="VFB53_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZN85804.1"
                     /translation="MTTKQLALVIDLNVCVGCHACVTSCKSWNNAGAAGPLTDQNPYG
                     ADPSGTFFNRVQTFEAGEFPATETVHFPKSCLHCEDPPCVPVCPTGASYKRAEDGIVL
                     VDYDKCIGCKYCAWACPYGAREIDEQQQVMKKCTLCVDRIYDEKLPAADRKPACVLAC
                     PTSARIFGDVHDPESEASRAIRERGGYALMPEWETRPANHYLPRRK"
     gene            29475..30344
                     /locus_tag="VFB53_03375"
     CDS             29475..30344
                     /locus_tag="VFB53_03375"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZN85805.1"
                     /translation="MRPAWSVLLFTTLAGAGQGLFLALFAAQPQGRLMQAGLATSLAL
                     LAAGLIASFFHLGHPERAWRAAAMWRTSWLSREVIVLPAFMALVAAYGWTQSALLGAL
                     AVAACLALFACTGMIYACLPFLQEWRSPLTLVNFTLLGCASGLTLAAALAAALEPALA
                     RGYVLAALAVGAAAYLGRLASLFRNARLRPKSTLASATGIPQRPIVQMSQGAMGGSFN
                     TREFFHGRPRAVVRAARWTLLALVFPVPAALLLAGQAAPAFLLQFAGLLAERWYFFAE
                     AKHPQNLYYQAIA"
     gene            30357..30584
                     /locus_tag="VFB53_03380"
     CDS             30357..30584
                     /locus_tag="VFB53_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZN85806.1"
                     /translation="MHHDVELDARGLNCPLPILRAKKALNGMQSGQVLKVVATDPGAV
                     KDFQTFAKQTGNELLQHAEADREFTLYMKRK"
     gene            30581..31648
                     /locus_tag="VFB53_03385"
     CDS             30581..31648
                     /locus_tag="VFB53_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZN85807.1"
                     /translation="MNAAWVTGGAFALAFVFGAVGNRTNFCTMGAVSDWVNMGDLNRM
                     RMWLLAIAVALAGSSALHLAGIIDLSKSIYPGPNFTWLSYLVGGFLFGVGMTLGSGCG
                     SKTLIRIGAGNLKSLIVYVFLGIGAYMTLKGLFGAFRVGVLEKAAVTLPAGQDLPALL
                     GLHRAVPAALIGGALLAFVYRSREFRAGFDYTLGGVVTGLVVAGGWVVSGVLGHVAEH
                     PETLQEAFIATNTGRMESFSFVSPMAYTLEYLMLWTDKSKIVTYGIASGLGVIAGSAA
                     YALATRSFRWEGFRDAEDTANHMVGGMLMGFGGITALGCTIGQAVTGISTLALGSFMT
                     FAAIVAGSALTMKYQYWRAGA"
     gene            31650..32135
                     /locus_tag="VFB53_03390"
     CDS             31650..32135
                     /locus_tag="VFB53_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN85808.1"
                     /translation="MKRVLALWLGCATLAAAADATHTVKLLTPETALKAALAAQKKCR
                     DGGHQATVAVVDRMGVTQVLLRDRFAGPHTVDTARAKAWTAASFRTDTTGLAEASQPG
                     RPASGIRNLPGVAAVGGGLMIQAGGSLLGAIGVSGAPGGREDDACAAAGIAAIRDELE
                     L"
     gene            32209..33240
                     /locus_tag="VFB53_03395"
     CDS             32209..33240
                     /locus_tag="VFB53_03395"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZN85809.1"
                     /translation="MDQALLSKTAAAMVARGKGVLAADESSATCEKRFKSVGADCTEE
                     SRRFYRGLLFTAPGVEQFLSGVILFDETLRQKTDDGVPFPEYMAKKGILPGIKVDKGA
                     MDLALCPGEKVTEGLDGLRARLAEYFKLGARFAKWRAVITIGDEGSTLPSAACIEANA
                     HALARYAALCQEASIVPMIEPEVLLDGGHTVERCEEVTEATLRATYAAMAAQSVAVEQ
                     LILKTSMVVSGKDCARQAGVDEVAERTLRVLRRTVPAAQPGIVFLSGGQSDEAATAHL
                     NAMASAPGLPWPLTFSYSRALQNPALKAWRGQAANAAAAQKAFCHRARMNGLAAQGKW
                     QAALEKQAA"
     gene            33240..34142
                     /locus_tag="VFB53_03400"
     CDS             33240..34142
                     /locus_tag="VFB53_03400"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZN85810.1"
                     /translation="MKIGSDPIFESSLKSLPRIGKGKVRDIYAVGEDKMLIVASDRLS
                     AFDVVLPDPIPQKGRVLNEMANFWFGKLGHIVPNQLTGIDPEEVVEGEDEKAQVRGRS
                     VVVKKLRPLPIEAVVRGYLIGSGWKDYQRTGKVCGIELPKGLQQAQKLPQPIFTPATK
                     AEEGHDENISFDEVEQLIGEKLANRVREISLRLYKEASEYAATRGIIIADTKFEFGTD
                     DKGGLVLIDEVLTADSSRFWPADSYQVGISPPSFDKQFVRDYLETMQWNKAPPAPPLP
                     REVIAKTSEKYQEALERLTGRKLA"
     gene            34139..34618
                     /gene="purE"
                     /locus_tag="VFB53_03405"
     CDS             34139..34618
                     /gene="purE"
                     /locus_tag="VFB53_03405"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZN85811.1"
                     /translation="MIGIVMGSTSDWETMKAAAKMLKEFGVAFEAKAMSAHRSPHAVV
                     KWTEGAEKRGLRAIIAAAGGAAHLAGVVAAHTTLPVLGVPMPSKHLNGLDSLLSTVQM
                     PKGVPVATFAIGEAGAANAALFAVALLALSDKALAKKLWKFRRYQTAAVLKAAPLKL"
     gene            34615..35550
                     /locus_tag="VFB53_03410"
     CDS             34615..35550
                     /locus_tag="VFB53_03410"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZN85812.1"
                     /translation="MRPEVRRAAEILERGGLVAFPTETVYGLGADASSREAVARLYAA
                     KRRPADHPVIVHFADAQGAFAWASAVPPAAMKLAAQFWPGPLTLILKRSAQAGDFVTG
                     GQDTIGLRVPSHPVAQELLRAFGGGIAAPSANRYGKVSPTTAAHVREDLGEDVALVLD
                     GGPSEVGIESAIVDLSGGRAVLLRPGGISRQQLAQALGETVEEKRADSPRHSGGHERH
                     YAPRTPARLVPTHTLDQEIAKLGSKVAVLAFSRPDERVDYWLRMPREPAGYAQKLYAA
                     LRELDGAQCESILIEAPPETAEWAGVRDRLLRATS"
     gene            35547..35852
                     /locus_tag="VFB53_03415"
     CDS             35547..35852
                     /locus_tag="VFB53_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85813.1"
                     /translation="MRILLLVSLLISGCSHTSVQGGWGGAGPSGGNVSVGVSGSAVGW
                     ALIGIGLIAAGEYAEQRRLDGLPPMNEPPRELDKGRRVNEQDCSQPIKDWSANLKCR"
     gene            35843..>36553
                     /locus_tag="VFB53_03420"
     CDS             35843..>36553
                     /locus_tag="VFB53_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN85814.1"
                     /translation="MPVGGFPEQRYLVGARELRERLGDVTLLDLRPAEDFAVGRIEGA
                     RHLDLYGVSLNDSSEAPLEAFLAIFRVLFGARGVTLDKPVVVYDHESGERASRAVWLL
                     TVLGHPDARLLDGGTQGWVAAGGKLARVDAAPPPVDPTKAPPTAPPFKGTMNMDLVAS
                     RFDVQRAIGNLEYVIVDVRRGSEYRGTEKRARRAGTIPGAVHVFWRDHLDAKGALRPA
                     EEVRALYASRGVTPDKNVI"
BASE COUNT         5401 a        12687 c        13013 g         5452 t
ORIGIN      
        1 ccttcatcga cgcgccgttc ctgttccgcg acctcgcgca ctggaacaag gtgctcgacc
       61 aggatctgct caagccgatc gccgacgaga tcgcgcagaa ggccgacgtg atgctgatcg
      121 ggtacgccgg cggcggcacg cgcaacatct tcatcaacaa gccgatgggc tcgctggccg
      181 agatcaaggg cctcaaggtg cgcgtgcagg gcgcgccgat ctggtccaag accttccagg
      241 ccgtgggcat ggcgccgacg gtcatcgcct acaacgaggt gtacaacgcg atccagaacg
      301 gcgtcatcgc cgcgggcgag aacgaggccg ccggcgtgga gcagatgaag ttctacgagg
      361 tggcgccgca cctgtcgatg acgcagcacg ccatcaccat ccggccgctg tgcttctccg
      421 gcaagacctt caagaagctc gacaaggccc tgcaggcggc ggtgctgaag gccggcaagg
      481 aggcgggcgc ctatggccgg cagatcgagt cgagcgagga cgaggcgaag ctcgccaaga
      541 tggagaagga gggcaagctc aagcgcgtgc ccttcgccgg gcgcgaccag atgaagaagc
      601 tggtggaccc ggtgatggcg gagtacgcca aggagatcgg cgccgaccag atcctggcga
      661 agatcaacgc catcaagtaa cgcgttgacc gaggcgaacg cggcggcgcc ggagcgccgc
      721 cgctttttta tgctgcgcaa gctcacgcac tggtacggca cgctgctgtc ctggctgctg
      781 gtggcgtcgg tggcggtctt ggtgttcccg gtgtcgctgc agatcttctc gcgctacacc
      841 gcgctgatcc cgagctacat ctggaccgag gagatggcgc ggttcttctt catctggtcg
      901 atcatgctcg gcgcgatggt cggcatccgc gagggcacgc acttcgtggt ggacctgtgg
      961 ccgccgatga acgcgcgcgc gcaggcggcg gtgcgcctgc tgtccagcgt gttcgtgctg
     1021 gccttcgccg cggtgttcct gtggtggggc atcgacttca cgcgcttcgc cttcaaccgc
     1081 atctccgagc tcgccgagct gccgctgtgg gtgatccacg tcgcctggcc gctcgccggc
     1141 gcgacctggc tgctgttcca gggcgagcac gcgtggcacg acctgcagct gctgcggcgg
     1201 ggcaaggcat gaccggcgcc acgctgctcg atcccgcgac cgccagcctg atcatgttcg
     1261 gcgctttctt cctgctgctc gcgctgcgcg tgccggtggc cttcgcgctg ggcctcgcct
     1321 gcctgccggt gttcctcatc gaggaccgcc tcacgccgca gaacctggtg caggagacct
     1381 tcaacgccta caactccttc atcctgctgg cggtgccgtt cttcctgctc accgccaacc
     1441 tgatgaacgt gggcggcatc accacgcgcc tggtgcgcct gtcgcgcgac ctggtggggc
     1501 atttccccgg ggggctcgcg caggtcaacg tggtgctgtc gatcttcttc gccggcatct
     1561 cgggctcctc caccgcggac gcggccagcc agtcgaagat cttcatcgag gcgcagcgca
     1621 aggagggcta ccacgacagc ttctcggtgg cgatcaccgc ggtgtcggcg gtgctggcgg
     1681 tgatcatccc gccctcgatc ctgatgatcg tgtggggcgg gctgctcacg gtgtcgatcg
     1741 gcggcctgtt cctcgccggc atcatccccg ggctgctgat cgggctggtg cagatggcca
     1801 ccgtgcatgt gtacgccaag ctgcgcaact acccgaccta tccgcgcgcc accctggtgg
     1861 agaccatcaa ggcgatcggc gtcgcgattc ccgcgctgat gacgccggtg atcatcatcg
     1921 gcggcaagat cttcggctgg ttcaccgcca ccgagtcggc ctgcatcgcg gtgctgtacg
     1981 cggcggcgct caccatgctc gtctaccggg aaatggactg gaagggcctg cacggcgcgc
     2041 tgctggaaac cgggcggctc accggcgtgg ccctgttctg cgtcggcacc gcgtccgcct
     2101 tcggctggct gctcgcctac taccagattc ccaaggcgct gctggcgggc gtggcggagt
     2161 ggggcatggg gccgatgacc accgggttct tcatcgccgg cgtgttcctg gtggtgggct
     2221 gcttcctcga cgcgatcccg gcgatcgtga tcgtcggcac ggtgctcgag ccgctcgcca
     2281 ggagcgtggg cctggacccg atccatttcg ccatgatcgg catcgtctcg ctcgccttcg
     2341 gcctggtgac cccgccctac gggctgtgcc tgatgatcgc ggcgcacgtc gccggcatgc
     2401 gggtggtgga tacgctgaag gacgtgttca tcatgctggt cccgatgctg gcggtgctgg
     2461 cgctggtgat catgtggccg gagctggtgc tgttcctgcc gaagctgttc ccgcccggca
     2521 cgctgtgagc gcgcccgatc ccgcgaagct gtccttgaac accgcgacga cgcgggagca
     2581 atggggcctc gcgcaggcca tcgagggctg cgcgcgccac ggcatccgcg gcatcgcgcc
     2641 ttggcgcgac cagctcgcgg cgatgggggt gaaggaggcg gtgcgggcga tcaagtcgaa
     2701 cggcctgacc gtcacgggat tgtgccgcgg cggcttcttc accgcgccgg actggaagga
     2761 cgacaaccgc cgcgccatcg aggaggcgca tgcgctgggc gcgcagtgcc tggtgctggt
     2821 ggtgggcggg ttgattccgg gatccaggga cctgccgggc tcccgcgaaa aggtgaagga
     2881 agggatcgcg gcgatcctgc cggaggcgag gaaggcgggc gtgccgctgg cgatcgaacc
     2941 cttgcacccg atgcaggcgg cggatcgagc ctgcgtcaat acgctggagc aggctttgga
     3001 catctgcgat gcgctcggcg atggcgtcgg cgtcgccgcc gacgtctatc atctgtggtg
     3061 ggatccgaaa ttggagagcc agatcgcgcg cgcgggaaag cagcgcctcc tcgcgtatca
     3121 catctgcgac tggctggtgc ccaccaggga cctcctgaac gaccgcggca tgatgggcga
     3181 cggcgtcatc gacctgccgg ggatccgcgg catggtcgag cgcgccggct acgcgggctt
     3241 ccacgaagtg gagatcttct cgaaactcga ctggtggcgg cgcgatccgg acgaagtgct
     3301 ggccacctgc aaggaccgtc acaggagaac ctgaccatgc gcggcgcagt gttcctcggc
     3361 aaccgcaaga tcgagatccg caactttccc gaccccgtgc ccggaccggg cgaagtcgtg
     3421 atccagatga aggcctccgg catgtgcggc agcgacctga agttctaccg gccgccgccg
     3481 ggagaggcgc aaaaggcgct ggggctgggc ggcaacgccg agcccttcat cggcggccac
     3541 gagccctgcg gcgtcatcgc cgcgcgcggc gcgggcgttt ccgagcgcga agcgcccatc
     3601 ggccagcgcg tcatgaacca ccactacgcg ggctgcggcg tctgcggcca ctgccgcgtg
     3661 ggctggtcgc agctgtgccg cgccggcatc gtcgtgtacg gggtcacggg caacggcggg
     3721 cacgcggagt acctgaaggc gcctgcgcgc accatggtgc cgctgcccga ggaactctcc
     3781 ttcgccgagg gcgcggcggt gtcctgcggc accggcaccg cctatggcgc cctgcgccgg
     3841 atgcaccttg cgggcggctc taccctcgcg gtgttcgggc agggcccggt cgggatcagc
     3901 gcgacgctgc tcggcgcgag gatgggcgcg cgcgtcatcg cggtcgaagc gaacgccgag
     3961 cgcctcgcgc tcgccaggac gtacggcgcc gacgcactgg tcgattcctc gaaggtcgac
     4021 gcggaacagg cgctgaggga tctgacgcgc ggcgagggcg tggacctcgc gctcgactgc
     4081 accggcgtcg cggcggcgcg cctggcggcg gtgcgctccg cgaagacctg ggggacggtg
     4141 tgcttcgtcg gcgaaggggg cgaggtgacc ctcgacgtgt cgcgcgacat gctgaggaag
     4201 cagctcacgc tcatcggctc ctggaccttc agcgccatgg gcatgggcga atgcgcccgc
     4261 ttcgtcgccg acaaccggat cccgctggag aagatcttca cccaccgctg gaagctcgac
     4321 caggtgcagg aagcctaccg cgtgttcgac gcgcagtcct ccggcaaggg cgtcctggag
     4381 ttctagaagt agggcaggcc ctgcgtgttc acgtacagcg cgtagatcga ggtgctcgcc
     4441 gccatgaaca ggcggttgcg gtgcgcgccg ccgaagcaga cgttggcgca gcgctcgggc
     4501 aggtcgatcc tcccgatcgg cctgccctcc ggattgaaga cgtgcacgcc gtccagtccc
     4561 gcctcgccca tgccccagcc gcaccagaga ttgccgtcga cgtccacgcg gaagccgtcc
     4621 ggcgttccct tgggcccggc gtcgatcagc acgcggcggt tggcgagcga cttcccgccc
     4681 ttcacgtcgt aggcgaggat cttgcgcggc accgagcgcg actcgacgac gtagagcagc
     4741 gactcgtcgg gcgagaaggc gagaccgttg ggctggttga tgccttccgc cacgacgctc
     4801 agcgctcccg acgggtcgat gcggtagacg ttggtcggca gctccgcctc ggccttcgcg
     4861 ccctcgtacc agccgaggat gccgaagctc gggtcggtga accacagcga gccgtcggac
     4921 ttgcacacca cgtcgttcgg cgaattgagc cgcttgccct cgtagcgctc ggcgagcgcg
     4981 acgatgcgcc cgtcgggctc ggtgcgcgac acgcgccggc cgccgtgctc gcaggtcacc
     5041 aggcgcccct ggcgatcgcg cgtgttgccg ttggcgtagc ccgaaggcct gcgccaggcg
     5101 cccaccgcgc ccgtcgcctc gtcccagcgc cacatgcagt cgccgggtac gtcgctccac
     5161 acgaggcact tctggtcgcc gaaccacacc ggcccctcgg cccagcgcgt gacgccggtg
     5221 gcgaggcgct cgaccttggc gagcggcagg cggtagcggt tgaaggaatc gtccatcgcc
     5281 acgatggacg ggtcggggta gcgcgggctg ggctgccagg tcgcgctcac cgctaacgcc
     5341 ccgagcgcgc gagcagcgct tcgaagccgt tttcgctgaa accgctcatc gccgccctgc
     5401 cgtggacgat gagcggaacg cccttgccgc cgaggcgcgt gaattccgcc ttggcctcgg
     5461 gagatttctc cacgtcgtgc tcctcgtagg cgatgccgtg cttcctgaag taggcgcgcg
     5521 ccttggcgca gaccgggcac caggaggtcg cgtacatcac cagcggctgc gcggccgcgg
     5581 cggccggcgc cttcaccact cccggcacgc ggcgcacgtc caccgggccg gcgtaggtgc
     5641 tgacgcgctc gggcacctgc atcgcgcgcg catcgcgcgg cggctcgttg ccgtagtgca
     5701 cccggccccg ttcgtcggtc cagcgatgga tgccctgcgc ggccgcggcg cccgcggcga
     5761 gcagcgcaag caccccccca aaggtttgcc agcctcccat gcgcgccctt agtagcatag
     5821 gtccggggac gcgggcaatg gctatactcg accacggtgg ctcgcatcct gctgacccat
     5881 tcgcccgaga ccctggagcg ctactacggc gcgcgcgcgc tcgccgggct gcaggggttg
     5941 ggcgaggtgc ggctgcaccg gggcggcgcg ccgctcgagg gcgaagcgct catcgcggcg
     6001 gccgccggct gcgagctcat cgtctcctac cgccagtcga gcgcaccggc ggcgctgttc
     6061 gagcggctgc ccgcgctggc cgccttcctg cgctgcgcgg tggacatccg caacatcgac
     6121 gtcgccgccg cgagccgggc gggcgtgctg gtgacgcagg cgagcgccgg cttcgtgccg
     6181 gcggtgagcg agctggtgac cgggttcctg gtggacctct cgcgcggcat ctcgcgcgcg
     6241 gcggccgagt accgtgccgg gcgcgtgccg gcggcgagca ccggccgcga gctgcgcggc
     6301 agcacgctgg gcatcatcgg ctacggcgcc atcggcaggg aactcgcgcg cgtggccagg
     6361 gcgctcggca tgcgcgtgct ggtgcacgat ccctacgtga aaggaatcga gcagacgccg
     6421 cgggatgacc tgctcgcgca gtcggactac gtcgtgccgc tggcggtggc gacgccggag
     6481 accgagaacc tgatggacga cgcggccttc gcgaagatga agcgcggcgc attcttcatc
     6541 aacgtgtcgc gcggcaacct ggtggacgag gcggcgctcg cgcgcgcgct ggactccggg
     6601 cgcatcgcgg gctgcgcgct cgacgtgggg cgcgcgcccg accagatgcc gacgccggcg
     6661 ctcgccgcgc gcgccgatgt catcgccacg ccgcacaccg cgggtctcac cacgcccgcc
     6721 atcgagcacc aatcgctgga gacggtggcg caggcaggcg aaatattgca ggggcgcgca
     6781 cccaagggcg cggtcaacgc cgaacactgg acaaggaagt cactgctcac accatgaaaa
     6841 ccatcctcat caccggcgcc gcgggcgacg tcggcaccca cctgcgccgc gaactggcgg
     6901 gcaagtaccg catccgcgcc tccgacctca ggccgctgaa gaaggtggcg caggagacct
     6961 tcatgcgcgc ggacatcgcg aaattcgccg acgcgctgaa gatcaccagg ggggtggacg
     7021 cgatcgtgca cctgggcggc tactcggtgg aggggccctg ggaggggatc ctcagcgcca
     7081 acatcgtcgg ctgctacaac gtgttcgagg cggcgcgcag gaacggcgtg aagcgcatcc
     7141 tgttccccac ctccaaccac gccaccggct ttttccggcg cgaccagacc atcgaccacc
     7201 gcgtgtacat caagcccgac agccgctacg gcgtctcgaa ggtgttcggc gaggcgctcg
     7261 gcagcctgta cgccgacaag tacggcatgc aggtgttctg catgaggatc ggcaacgtca
     7321 acccgctgcc gatcgacaag cggcgcctcg ccatctggtt ctcgccgcgc gacctcgcgc
     7381 agctggtgac catcggcatc gagcacccgg agatccgctt cgagatcgtc tacggcgttt
     7441 ccggcaacca gcgcagctgg tacgacaacg cgaacgcgtt ccgcctcggc tatcggccgc
     7501 aggacgactc ggaggtctac gcaaaggagg tcctcgcgcg cgagaagccc ggcggccatc
     7561 cggtggcgga agcgcaccag ggcggggtgt tctgcgtcgc ggaggaggtg cccaatcccg
     7621 cgccgctgcc gcggctcagg aagcgcgccg cgcggacgtc gtgaggtaaa ggccgagcac
     7681 gatgcagccc acgccggcgg cgtggtgcca gccgatgcgc tcgccgagga acgcgatgcc
     7741 gagcagggcg gtgaaggcgg gatgcaggtg gtggaagggc ccggcgaccg tcgcggtcgt
     7801 gcgctgcagc gccgcgttgt agaacacgaa ggcgaggaac gaggcgaaca ccgcggtgta
     7861 ggcgatcgcc gcgagcgacg cgggagtcac gaccatgtgc cggcccgcgg cccactccca
     7921 ggcgaagaac ggcgactgca ccgccacgcc gaccagcgcc atggcggcga ggaagctcat
     7981 gcgctccagc cccgccggcc agcgcttgag cagcaccgtg tagagcgccc agatcggcac
     8041 cgcggcgagg atccacaggt cgccgggatt gaaggcgagc gcgcgcagca ccgacagctc
     8101 gccgcgcacg atgaccacca ccgcgccggc cagcgaaacc aggatgccca gcccctggcg
     8161 ggcgccgatg cgctcgccga ggatcgccgc ggcaatggcc acgaccagta ccggcaccag
     8221 cgcgatgatc agcgcgccgt tcagcgcggt ggtggactgc agcccgaggt acaccatgct
     8281 ctggaagatc gccgaggcga tcgccgccat cggcagcacc cacttccagt ggcgcaggag
     8341 gagcggccat tgccggcgca gcgccggcca ggcgaccggc aggatcagca ggagcgagcc
     8401 ggcccagcgc cagaaattga gcgtgaaggg cgggacctgc tcgtggatcg cgcggcccac
     8461 cacccagttg cccgcccagc agagcatggc gagcatgagc aggagggcgg gcgagcggag
     8521 gtggtgcacc cctacatctt cggtgttttc gcgccgcggt gccggtcggc ccaccaggcc
     8581 caggcggtga acaggatgcc gcccgcgagg ccggcgatcg cgatcgccgt ctcgccgggc
     8641 ggcggcggcc cgaacacgtt gcccaggtag gcgacggcga ggaaggcgat cagtccccag
     8701 aacgcccagg agccgacgcg gtcctgcgac ggcgcgctgc gcgcgtagag ccacacgccg
     8761 gcggcgaaca tcgcgcattc cacggcgatg gtcgccggca cggagttcca caggcccaag
     8821 ccgatcatcg cctcgctgcc cggccacagc ggcaggtcgg gccggtgcgt gaccgcatcc
     8881 agcacccagt ggctgaacac cagcagcccg aggatcaagg cgacgcgccg gccgctgcgc
     8941 aggcacgcca ggccgaacag gacgctccag cccagcgcgg cggccaggct gtgcgtccag
     9001 gggtaatgca ggaaatcaag cggcgtgaag gcggtgttgc ccgggtcgat cttcacccgt
     9061 tcgatgccca gcagcaggaa cagcggccac accatgtcca cccagaacgc cgccgcgagc
     9121 agcagcgcga gcgaggtgcg cggcgccaga ggcttggcgg caaacgcgac ggcggcgtgg
     9181 ccgatgaaca ccgctcagaa gctcgcttgc gcgcgcttgc actccatcac gccggcgcgc
     9241 ccggcggtct cgtccatcaa ctgcaggttc gagcgcgcct gcatcaggcc catcttcact
     9301 tcctgccaga cgtagtagtt gcgcccggcg ctggcgttca cggtgagctc ggcgacgttc
     9361 tccgtgtgcg acgccagccg gtggctgccg ggatcgacct cgaacaggaa gtaggtcttc
     9421 gacgccgtca gcccggcgat gcgcccgttg agcgacaccg gcatcttcac cgccgcgccc
     9481 atggtctcgt ggcggtagac gtagatgttc gccttcccct ggcgcggcat gaagcccttg
     9541 gcggcgaggt cctcggcgtc cgagcccatc ggcacgctgg cgcagcccgt caggaccgcc
     9601 agcaacgcaa cgcaaaacat accagcaatt cttttcacgc tgtcccccat cgatgaaccg
     9661 gggtcagagc ccggtttccg gattggcgaa atatcgggcc ctgaccccga tgtcctcagt
     9721 cgtccgcatg cgccgcgggg cggtgcgccg ccaccagctt ttgctgcagc tgcggcggcg
     9781 ccggctcgta gtggctgaac tcgagggtgt aggagccgtg cccggcggtg agcgacttga
     9841 ggcgcgccgg gaactgctcc agctcggcga gcggcgcctg cgccgcgatc tgcacggtgc
     9901 ccgggcgcgg cgcgtccgag ccgcggatgt ggccgcggtg gccggagagc tcgccggtga
     9961 tgtcgcccat cttgtgctca ggcgcgacga tgctcacgtt gacgatcggc tcgagcacga
    10021 tggcgcgcgc cttggcgagc gcgtcgagga aggccttgcg cccggcggag acgaaggcca
    10081 cttccttgga gtccaccggg tggctcttgc cgtcgtagac gatcacgcgc aggtcctgga
    10141 tcgggtagcc ggcgagaaag cccgtgccga gcacgccgcg cacgcctttt tccaccgccg
    10201 ggatgaactg gttggggatc gcgccgccct tcaccatgtc gacgaactcg aagcccttgc
    10261 cgcgcggcag cggctcgacc ttgaggaaca cctcgccgaa ctgccccgcg ccgccggtct
    10321 gcttcttgtg gcggctgtgg ccctcggcgc gcgaggcgat ggtctcgcga tacggaatcg
    10381 agggcgggcg cgtgtcgagc tcgaccttgt actggttggc gatcttgtcc agcgtggtcc
    10441 tgaggtgcat ctcgcccagg ccgcgcatca ccgtttcgtt ggcctgcgca ttgtgctcga
    10501 tgcgaaagca cggatcctcg gcgaccagct tgtgcagcac gtccgacacc ttctgctcgt
    10561 cgccgcgctt cttcggctgc accgccagcc cgaacaccgg caccggcagc tcgagcggcc
    10621 gcgcgcggat gtgcgcgtcc ccgggcgagt cgtgcaggat ctggtcgaac tggatctcgt
    10681 ccaccttggc gatggcgcag atgtcgccgg gcaccgcctc ggcgacctcg gtctgggact
    10741 tgccgcgcag caggtacagg tgccccgcct tgaccggctt cctgccctcg ccgaggtaca
    10801 gctgcgtgcc gggcgtgatg cgtccctggt gcacgcggaa caccgccagg cggccgatgt
    10861 agggatcgat ctcgatcttg aacacgtggg cgagggcgtg cttgccggga tcgggcagcg
    10921 cctggatctc ggccggcgcg ccgccgtcca ccggcacgct gaggtaggcc ggcgggttgc
    10981 cttccgaggg attgggcatc agcttgacga tcacctcgag cagctcctgc acgccggcgc
    11041 cgctcttcgc cgagcagaac accaccggca ccaggtggcc ttcgcgcatc gcgcgctcga
    11101 gcggctcgtg cagctcctcg ggcgacacct cgcctttctc caggtagctc gccatcagct
    11161 gctcgtcgac ttccaccacc tggtccacca ggcgctggtg cgcctcggcc accgaggaga
    11221 aatccgcctc gccggcgggc gcgaaaaagc agtcggacac cttcgccgcc ttcgccgcgg
    11281 gcaggttgag cggcaggcac tccttgccga acgcctgctg gatgcgtccg agcagcccct
    11341 ccaggtcgac gttctcggcg tcgatcttgt tcaccaccac cagccggtcc aggccgcgct
    11401 tcgccgccca ctgcatgaag cgcccggtca tcatctcgat gccgttctgc gcgttgatca
    11461 cgatggcggc agtctccacc gcagcgagcg cgggcagcgc gtggccgagg aaatccggat
    11521 agccgggggt gtcgagcagg tgcacgaacg tcggctgctc gccgccgttc accgcgaagc
    11581 tcgccaccgc gagcttgagc gagtgctgca gctgcttttc ctgcggcgtg tagtcgcaga
    11641 ccgtggtgcc gcgctcgacg ctgccggccg cggcgatcgc cccggcgcgc gcgagcagcg
    11701 cctcgaccag gctggtcttg ccgctgcccg cctggccgac gagcgcgagc gttctcaggt
    11761 gcttgatcag gtgcctggcc accgccccgc ctcctgggac ctatgctact gcggcgggcc
    11821 gggcgcgcgc tacttcctgc ccgcgccctt ctgctccagc agctcggcgc gctttttcag
    11881 gatgtcgtcg aatttcgact tggcgatcaa cagcgtatgc gcggcgaggg cgcgctcggc
    11941 cggcaggcgc gcggcgagcg tcttctgccg ctcggcggcg tcggccccgg tcgccttcgg
    12001 ctcgccggcc accgccacgg cggcgtagta gttctcgccc gcgggcttgc cgagccgcac
    12061 ggtgtaggtc aggccgtcga aggtgcgcgc gacgatggtg gcgaagggcc ggtcgaggcc
    12121 ggtgtcggcg ctcttcaggc cgggcgcggc gacatccgcg agcgccacgc tgttgagcga
    12181 gtaggaagcc gaattggcgc gcatcgcgtc gagcttttcg ccggcgcgcg aggggctgag
    12241 ctgccagccc gcatcgtcgc gcgcccgctc gatgcgccat ttctcgccgc ccggcagcgc
    12301 ggtttccatg gccatcacct tctcggcggc aaagccggtg cgctcgatcc agtcggcgct
    12361 cctggtgctg gcgagcgtca gcgggtccga caccacgatc gcggtctgcg ggtcctcggg
    12421 caactgcacg aagcgcccgt cgccgatggc gagcgccgga ttcgccggct cccgcttgaa
    12481 aaacttgctg ccgacggcca gacgcgcgag cggcttgccg tcggcgccga ggaattcgag
    12541 cgccgtgccc gagccgtcga gcttcaggcg cgcgcggtcc tgcccgccga tcggttcgct
    12601 ctggcccacc ttgagctcga gcgccttcag caccagcgcg cgcaccttgt cgaggtcggc
    12661 gggaaagccg ccgcgctcgg cgatggtcca gcggccggcc tggttccgca ggctgaggct
    12721 cgcgccgggc tcgcgcagga cgatggcggc gatgtcggcc gccttgaggt tcttcagcag
    12781 cggctggccg agggcgccgg cgcccgcggg gcgctcggcc gcgcgctgct gctgcagcag
    12841 cagcgcgccg ccgcccaggg cggcgagcag caccagcagc acccccgcga tgcgcgcgtt
    12901 catgcggcgg cgagcctcgc ctgccggcgg cggcgcagca gcgcgaccag caggccggcg
    12961 agcgcgaccg cgagcggcat cagcgcgatg ttgacgagct tggtgaggaa caccaggcgc
    13021 tcgctgtcct ggcgcaggtt cttgcgcagc tccttgagcg ccttgcgcga ttcggcgagc
    13081 ttcttgcgga agctctccag ctccgcctgc tgctcgggcg agaggatctg cgccgccttg
    13141 gcgccggcgc cgggggactt ctgcagcttc tgcatgttct cggtggtctt ctgcagctcg
    13201 tcctcgagct ccttgatctt gccgaagtac tgcgcctgcg cctcggcctc catctcgcgc
    13261 accacggtga gcgggcggaa ggcggcggcg cggctcttca gcgacagcag gctgtcgtcc
    13321 gacgccagct gctcgaccag gccctgcgcg aaggcgaggt tgccgttgga gggcacgacg
    13381 atgcgccggc cgaacacctc ctgcacgtcc accgccgcgc cgtcctgcag catgtccgcg
    13441 tcggcgacca gcacgacggc gccgtcgcgc gccgagacct tcaagggcgc cgcgtccgcc
    13501 gcccctggtt tgggcttggc gttcttgtcc acgggcctgc ccgcggggaa ggcggtcttg
    13561 aatttccccg cgaggcgcag cgcgagcagc aggggcttgc cgccgggctg gaagctcctg
    13621 gtcgcggcgt cccccgccac cgtggcgctc gcgctgtcca ccagcatcga gttgggggag
    13681 ctgcgcacca gctcgctcgc cgcgaggccc tcggcgggct tcaccgcgaa ggcgccgccg
    13741 aaggcgtaga gcagggtctc gatctggctg gcgacgacgt cgtcgcgcga gaaggcggtg
    13801 cggttgagcg agagcaccgt gggcgtatag cgctgcccgc cgcccgaggc gaacaccacg
    13861 tcggacacca ccttggcggg gtccatctcc acgccccagg ccttgaacag gcgcggcagc
    13921 gtcgagctcg aggcgaaggc cggcatgccc ggcatcggcg gctgctgctg gtcgaagtag
    13981 gcgtaggggt cgacgaaggc gatgagcctg ccgccgcgca gcacgaactg gtcgagcgcg
    14041 aactcggcga gctcggagat gtcgcgcggg tggacgagca gcagcacgcc gaggtccttg
    14101 tcgatcgcgt cggtggtcat gttgacctgc accacgtcga agtcgcgctg cagctcgctc
    14161 gccagcaccc agggctcgga actctggcgc gtcatcgggt tgaagcgctc gccgagcacc
    14221 ggcaggccgc tcatcacgcc gatcttcggg cgctcggcga tgccggcgcg cgcgatggcg
    14281 cgcaccaggt cgtactccag caagcgctcg cgctgcggcg ccaccaccgg gatcgactgc
    14341 ttgcggtcga gctggctcac cgccaggccg aggtagaact gctcgcccga ggcgagctgc
    14401 tgcggctcga tgccgtcgag ctgcgccgcg tcctcctcct cggagtcggg ccgcgggtcg
    14461 tacttctcga tcaccagctt gtcgccggcg acggacttga actcgcgcgc caggtcctcg
    14521 acgcgccgcg cgaagctgcg cagctgcacc ggcatcgcct cgcccgcggt gaggtacagc
    14581 ttcaccttga ccggcgcctg caggccgcgc agcagcttct tcgtgccgtc ggacagcgtg
    14641 tagaggccgc cctcggtgag gtcggcgcgc agcgccgcgc ccgcggcgag gtagttgaag
    14701 gcgaccagca gcaggaacag ggccgccagt ccgaccgccg agtacagcag cgcttcgtgc
    14761 tttttcatcg gttcctcagc gcgacgccgg tggcgaacag cgcgaagccg atcaccgaca
    14821 ggaagaacag caggtcccgc gagtcgatca cgcccttctg gaagccctcg aaatgcgtga
    14881 tcaccgagaa ggcggccacc gtgtcgacga aggcggggct cgcccagcgc accagcaggt
    14941 cggtcaccgg gttgaagccg gcgaggatca ggaacaggca caccaccacc gcgaggatga
    15001 aggcgatcac ctggttgcgc gtcagcgcgg aggtgaggca ggtgaccgcg aggtaggcgc
    15061 cggcgaggag caggctcccc aggtagcccg cggcgatggc gccgttgtcc gggtcgccga
    15121 gcacgttcac ggtgaccgtc accgggaagg tgagcgccag cgcgatggcg aggaaggccc
    15181 aggaggcgag gaacttgccg aggatcgcct gccacgccgt gaccggcatg gtgagcagca
    15241 attcgagggt gccggcgcgg cgctcctccg cccacaggcg catgccggcg gcgggcacca
    15301 gcatcaggta gacccacggg tgccagctga agaaggcccc caggctcgcc tcgccgcgct
    15361 cgaagaaggc gccggcggtg aaggtgagga agcccgtcag cagcaggaag atcaccagga
    15421 atacgtaggc caccggggag atgaagtagg acgcgagctc ccgcttggcg atggtgcgga
    15481 tggcgtgcca ggggttcatg atttcttcac cgtgtccggc agcgtgatgc ggcggaacac
    15541 ctcgtcgagc ggccggccga gctcgtcggg cgtgccgctc gccacgatgc ggccgcggtc
    15601 gatgatgatg gcgcggctgc acacggcgtc gacttcctcg aggatgtggg tggagaagat
    15661 gatcgccttc gtcctgccga gctcgcggat caggttcctg atctcgtgct tctggttcgg
    15721 gtcgaggccg tcggtgggct cgtccaggat cagcacctcg ggatcgtgga tcagcgcctg
    15781 cgcgaggcag gcgcggtgct tgtagccctt ggacagggtg tcgatggtct ggtgcagcac
    15841 cgacgcgagg aagcagcgct ccaccgcgcg gcccactgca tgcctgcgcg ccgcgccgcg
    15901 caggccgcgc atctcggcgg cgaaggcgag gaaaccctgc acggtcatgt cggggtagga
    15961 gggcgcggcc tccggcaggt agccgatgag gcgcttggcc gcgagcggcg cctccaccac
    16021 gtcgtgcccg cccaccgtca cgcggccgct ggtgggcggg aaatagccgg tgatcatgcg
    16081 catggtggtg gacttgccgg cgccgttcgg gccgaggaag ccgagcactt cgccgcgctc
    16141 caccgcgaag gacacgccgt ccacggcgcg cttggcgccg aaggccttta ccaggttttc
    16201 gatgcggatc atcgggtcaa cggcgcttgc ggcggggttt caagggggcg cattatcgca
    16261 aagaatcgaa cgtgcactag aattcgttcg accatccccc ggaggcagac catgctcaag
    16321 ctcgcaaagt tcctcgtact gatgacgttc gcggccgcgg ccgcggccca gcccagcctg
    16381 aagatgatga ttccggccaa ccccgggggc ggctgggacc agaccgggcg caacctcgcc
    16441 gcggccatgc agagcgccaa gctggtgtcg ggggtgcagt tcgagaacaa gggcggcgcc
    16501 gcgggcgcca tcgggctggc gcagttcgtc aacgccgcca aaggcgaccc gaacgcggtg
    16561 atgatcggcg gcatggtgat ggtgggcggc acctacctgc agaactcccc ggtgacgctc
    16621 gcgcaggtga cgccgatcgc gcgcctcacc agcgaatggg aggtgatcgt ggtgccggcc
    16681 gcctcgccgc acaagaccat ggccgacctg gtggcggcgt tcaaggccga cccgggcaag
    16741 gtgtcctggg gcggcggttc cgcgggcggc accgaccaca tcctggcggg cctcatcgcc
    16801 caggcggtgg gcgtcgaggc gaagcgcgtc aactacgtgc cgttcaaggg cggcggcgag
    16861 gcggtggcgg cgatcatcgg cgggcatgtc accgccggcg tctcgggcat cggcgagttc
    16921 gccgagcacg tgaagagcgg caagatgcgc gccctcgcgg tgtccgcgcc ctcgaagacc
    16981 gacggcatcc cgagcctgaa ggaacagggc atcgacgtgg tgctcggcaa ctggcgcagc
    17041 atcttcgccg cgcccggcat cacgccggcg cagcgcgacg cgctggtgaa gctggtgcgc
    17101 gacgccacca acacgcccgc ctggaagggc acgctggaga agatgggctg gtcgggcgaa
    17161 ttcctcgccg gcgaggagtt caaggccttc ctcgaggccg accagaagcg catcgccggc
    17221 atcatcgact cgctcggcat caagaagaag tagcgcgcgc gtgcgccgct cgcgcgccga
    17281 gatcgcgctg tcgatcggcg tgctcgggct gggcgcgggc gcggcctggg tcaccgcgcg
    17341 gctgccggga gagggcggct acgccggcat cgggccgaac ttcatccccg cggtggtgtc
    17401 gggcgggctg ctgctgatgg gggcgtggct cctgtgggag gcgctcgccg gcggctggcg
    17461 caacctggcg gcgcccgagg cggacgccga gcacccgttc aacgccgccg ccttcggctg
    17521 ggtgagcgcg ggcctcttcg cgcacatggc gctcatcggc tgggcggggt tcgtgctcgc
    17581 cggcgcagcc ttgttcgccg gcgtggcgcg cggcttcggc agcccgcgcc cggcgcgcga
    17641 cgcggcgctg ggcttcgcgc tgtcgctggc ggtgttcctg ttcttcgtga agctgctcaa
    17701 cgtcaacctg ccggcgggct ggctgcagcc gctgctgggc ggcgccggaa tctagcgtgg
    17761 aaaccttcaa cgcgctgctg cacggcttct ccgtcgcgct gacgccgatg aacctcatgt
    17821 ggggcttcat cggcgtgacc gtcggcacgg cggtgggcgt gctgccgggc gtcggcccgg
    17881 cgctcaccgt ggcgatgctg ctgccgctca cggtgaagct cgaccccacc ggcgcgctga
    17941 tcatgttcgc cggcatctac tacggcgcga tgttcggcgg ctcgaccacc accatcctgc
    18001 tcaacacgcc gggggaatcg gcctccatcg ccaccgcgct cgagggcaac cggatggcca
    18061 agcgcggccg cgccgggccg gcgctcgcca ccgccgccat cggctccttc gtcgccggca
    18121 gcatcgccac ggtgctgctc acgctgctcg cgccgctggt ggtggagctg gcgctgaaat
    18181 tcgggccggc ggagtacttc gcgctgatgg tattcgcctt caccacggtg gccgcggtgc
    18241 tcggcagctc gaccgcgcgc ggcctggcga gcctgttcct ggggctcctc ttcggcctgg
    18301 tgggaatcga cggccagacc ggcgccgcac gcttcgcctt cggcgtgccc gagctgctcg
    18361 acggcatcga cgtggtggtg ctggcagtgg gcctgttcgc ggtgggcgag gcgctctaca
    18421 tggcggccta caccagccgc ctgccgggta cgctggagaa gctctccggc tcgctgatga
    18481 tgtccaggga cgactggaag cgctcctggc cggcgtggct gcgcgcgacc tgcatcggct
    18541 ttcccttcgg caccattccg gcgggcggcg cagagatccc caccttcctc tcctactcgg
    18601 tggagaggaa gctcagcaag cacccggagg agttcggcca cggcgcgatc gagggcgtgg
    18661 ccgggccgga agccgccaac aacgcctcgg ccaccggggt gctcgtgccg ctgctcacgc
    18721 tcggcatccc gacctcggcc accgcggcga tcctgttgag cgccttccag aactacggca
    18781 tccagcccgg gccgctgctg ttccagtcgc aagccgacct ggtctggggc ctgatcgcga
    18841 gcctgtacgt gggcaacgtg ctgctgctgg tgctcaacct gccgctggtg ggcatctggg
    18901 tgaaggtgct cgccatcccg cggccgatgc tctacggcgg gatcctggtt ttcgccacgc
    18961 tgggcgccta cagcctgcac cagtcctggg tggacctcgc gacgctctac gtcttcggcc
    19021 tgctcggctt cgccatgcgc cgcttcgaca tcccggtggc gccggcggtc atcggactca
    19081 tcctcgggcc gctcgccgag acgcaattcc gccgggccct ttccatcagc cagggcgacg
    19141 ccacggtgtt cctcacgcag ccgatctcgg cgtcgttcct cgccgtgacc gcggccctgg
    19201 tgatcgtgcc ctggctggtc aggcgctacg ggcgcaggta ggcgtagccc tcgcgctgct
    19261 gcaggcgcgt gatctcggcg acgccggaag gcacgaagcg cgcctcctcg atgaactgcc
    19321 cgcggccgag cttgcggttg cgcagcgtga tctcgcagac gtcgaactgc acgccgcgct
    19381 ccttcaggcc ttccaccgcc acctggtagg ggttgccgtt cctgtcgcgc gcgtccttga
    19441 acaggaagtc cacgccctgc gcgtgcgtca ccaccacgat cttcgccttc gggttcactt
    19501 ccaggtggtt gccgatgttg cgcagggcag ccatcgcctg ctgcgcgctg tcgttgacgt
    19561 ggtagacgac cttgtcttcg gcgacgttcg ccttcgggtc gcccaagctg ccgcagcccg
    19621 cgaggagcgc gaggccgagg accgctatcg cattgcgcat gtgcaggtct cctttacttg
    19681 ttgccgagga cccagcctac catcgcctgc aggtcggcat ccggaatgcc ggggttgggc
    19741 ggcatcggca cctgccccca gtttcccacg ccgccggcgc ggactttcgc cgccaggcgc
    19801 gcagccgcgc cggcatcgcc cgcgtacttc gccgcgacct cccggaacga gggtccgacc
    19861 acccgcttgt cgggcgcatg gcagccgacg caggcgtgcg cgttgaggag cttctgcacc
    19921 gggtcgtcgc cgcccccggc gagcgtgtcg ggcgagggcg gcggcgcggc ctgggcgcgg
    19981 ttgtagtagg tcgacaggta gcgctggatc acggccatct ccgcctcgtc catcggcgtg
    20041 ccgcgctcct tcatgttctt caggttgtcg gcccactcgc cgggcgagag ctgcgcgcgc
    20101 tggatgtgct tcagctcgtg gcagatcctg cacttgttct cggtgagcgt ggagcccggg
    20161 cccgagatca gcttctcctc ggcctgcgcg gcgccggcga gcagcgccgc gcacagccac
    20221 agctcaggcc gcaaccacga ccccgacgcg gtgccaggcg ttccagaaat aaccgagcgg
    20281 gttccaggcc ggcagcgcgg gctgcgcgtt gccgcggtcg tcccaggcgc gcgcgaggaa
    20341 ggtcacgtag cccggggcct cggccttgtg ctcgtaggtg aagctgcgcc aggcgtggcg
    20401 gccgctcccc ttcgcgagct gggcggggcg ccaggtcttg ccttcgtcga cggacacctc
    20461 gacgcggtcc accgaaccct cgccgaccca ggccttgccg cgcacggcga tcgcctcgcc
    20521 gggcttgaac cgcgcgttcg gcgcggggaa ggtgatcatc gacttgaccg gccaggcctc
    20581 cgtggatacc gcatccggcg gcatcttggt gccgggctcg acggggaacc tggggatgcg
    20641 gtagctcgaa tccatgaacg tgcccttgaa gggcgcatcg agcaccgtga gcgtgtgcag
    20701 ccacttggtc gaggccgagc ccacccagcc cgggaccacc aggcgcagcg gatagccgtg
    20761 cacgaagggc agcggcgcgt cgttcatcgc ccaggcgacg agcgtgtcct cgtccatggc
    20821 cttcttcatc gggaccgagc ggatcaccgg ggcggcggtg gccaccgccc ccggatcgcc
    20881 gccctggccg gcgacatgcg ccgcgcccgg cttcaggcct gccgcctgca gcaggtcgcg
    20941 caggcgcacg ccgccccact tcgggcagcc catgccgccg gtcggcagcc agggcgtgcc
    21001 gctcgcccgc ggctggtacc cggagcgccc ggagccggcg cactccagca tgccgtggat
    21061 cgagactgtc ttgaactcct tggccaactc gaacaccgag aaagagagct ccttgtcgac
    21121 caggcccttg agcgccagcc ggtggccgtt gatgtcgatc agcggcgtgg tgtggttgtt
    21181 gcgcacgaac atgcgctggc tcggcgtgac ctcgaagtcg tgctcgtgcg cggggacgct
    21241 gccggtgagc gggcgctccg agtgccattc catcgtgggc gccttcccgg ccttgacgtc
    21301 ctcgttggcc atggtgcccg gggccgtcgc cggcgccgcc tgcgccagca gttcgcccgc
    21361 cgtcgtcgcc agcgctccgg cgcccgccgc cgcgcgcagg aagccgcggc gggtcaacga
    21421 accttggtga tctcgcatgg gggcggtctc ctctgtttgc ctagacaggg aaagctaggc
    21481 caaatcgcgc ggcgaatcaa tatatggata tgcagcgtgc cgcgatgcga acgcgcttgt
    21541 gcggcgcagt gaaaattgag tacgctaccc ggtcggaaac ccgcaggagg catcgccaca
    21601 tgcaaggcac gcgaagaaac ctactccgcc tgggctggtc gatggggctg ctcgccgcct
    21661 tcggcgcggc gctcgtcagg cccggccgcg cgctcgccgc ctggaacaag ggcgctttcg
    21721 aggcgaagac ggtggccgag ggcatgcagg cgctcggcat cagcggcgcc gtggaatcca
    21781 aggacctcgt gatccgcgcg cccgacatcg ccgagaacgg cgcggtggtg ccgatcgaca
    21841 tcaccagcgg catcccgggc accaccgcga tctcggtgtt catcgagaag aaccccttcc
    21901 cctacgcggg cagtttcgac ttctcgcagg gcgcggtgcc gttcgtgcac ctgcgcgcga
    21961 agatcggcga gtccacgccg gtgcgcgtgg tggcggcggc gggcggcagg tactacagcg
    22021 cggtcaagga agtgaaggtc accatcggcg gctgcggcgg ctgaggcggg cgaggacatg
    22081 gccgagccga tgaagatccg cgccaccctg cagggcgacg tcgccgacgt gcgcgtgctg
    22141 atggcgcacc cgatggagac cggccagcgc aaggacgccg ccggcaagat cgtgccgctg
    22201 catttcatcc agagcatcgg agcgacgctg aacggcaagc ccgtgttcag cgccgacatc
    22261 agccaggcga tctcgcgcaa cccggtgttc gccttcaagg tgaagggcgc cagggccggc
    22321 gacacgatcg ccatcacctg gaccgacaac aagggcgaga cgcgcaccga ccaggcggtc
    22381 gtgcgcgccg cgccctgacc ggaggagcgg catgagacgc gcattggtgc tggcgctggc
    22441 cgcggcaccg tggctgcacg cggcggcgca ggcggaggat cccgtcgccg ccgagttcga
    22501 caagttccgc gcggtgctgg agcaggacaa ccccgcggag ctgttcgagg tgaagggcga
    22561 ggagctgtgg acgaagccgc gcgggccgaa gaacgcctcg ctcgccgcct gcgacctcgg
    22621 cctcgggccc ggcgtggtga aaggcgccta cgcgcagctg ccgcgctact tcgccgacgt
    22681 cgatcgcgtg atggatgccg aggcgcgcat cgtgcactgc atgatcacgc tgcagggcta
    22741 cagctacgcg gagctggcca agcagccgtt cagcagcctc acgcgcacgc cggacccggt
    22801 gtcgatcgcc acctacgtgg cggcgcagtc ggccgggatg aagatcgccg cgccgcaagc
    22861 gcacccgaag gagcgcgccg cctacgccgc cggccaggag atgttcttct accgcgcggg
    22921 tccctatgat ttctcctgcg ccagctgcca cggcgtggac gacaagcgca tccgcacgca
    22981 gccgctgccc aatttcacca ggccggcgca ggcggtgcgc gcggtgcagg gctggccggg
    23041 ctaccgcatg accggcgggg tgatgcacac gctgcagtgg cgcatgaacg actgcttccg
    23101 ccagcagcgc ttccccgagc ccggctacgc ttccgagatg gtgagcgcgc tgctcaccta
    23161 tctgaccgtc aacgccaacg ggcatgagta ccgcggcccg ggcatcaagc gctagaggga
    23221 acgcgatgcg acccgccgcc atcgcagtgc tcgccgccgc ggcgctcgcc gccgcctgcg
    23281 ccgggctgca gtccgacccg cgtgccgacg cgcgcgcgct gatgcagcgc gacttccacg
    23341 ccaagggcag cgccggcatg gaccggttga aggaggaccc cgtgcagtcg gcctgcaacg
    23401 cgcaccgcga caacccgccg caggcgctgg ccgaaaagct gcagcaggag cagatggcgg
    23461 ccatcaagtg gcccgcggac ggcaagctgc tcggcgactg gaagtccggc gagaaaatcg
    23521 cgcaggacgg tcgcggcatg gcctggaccg acaagggcgg cgtcggcggc agctgctaca
    23581 actgccaccg catcgggccg gccaccactt ccttcggcac catcgggccg agcctgcacc
    23641 acttcggcaa gctgcgcggc tacggcgcgg agatgcagcg ctacgcctac ggcaggatct
    23701 acaatgccaa ggcctacaac ctgtgcacgc agatgccgcg cttcggccac gccggcgcgc
    23761 tcagcgagca gcagatgaag gacctcaccg cgctgctgat ggacccggcc tcgccggtca
    23821 accagtaaac gggcggcgcg cgcagtggcg tgaagggagg cgccgatgcg ttacgcctgg
    23881 cttgcggctg tagcggttcc cctggcactg gccggcgcgc cggccgcgca ggccagcgac
    23941 cgcggaccct acgtcgccgt gatgatgacc ggcagcgagc cgagcggccc gaagccgact
    24001 gcgagcacgg acctggacgg cttcagcggc gtcgggctct ccgccgccgt cggctaccgg
    24061 tggcttcccc tgcgcgtgga gctcgagtac cagggcaaca ccgcctactg gatcggttcg
    24121 ttgttcagcg gcagcgggga tgaccgcatc cagatgaaga cgctcatgct caacgccatc
    24181 gtcgaggcgc agctcaacgg gtggctgggc gtatttttcg gggcgggatt cgggcaggcg
    24241 gacgtcaggg ccgattttgt cacctgcctg cagctgcagg gctgtctcgc gcccgccgag
    24301 tcgcacgcgt cgggatccgc cagcgcccgc cagacccagc ttggcgtgac ggtggggccg
    24361 ttccaccagc atcaggtcgt gatcgggttt cgctggctca agtcggggcg cctgggcctc
    24421 gccgataccg ccgggcggcc gttcgaggag gatcgcgcca aggcgaagat gtcatttgtc
    24481 ggctggcgcg ggagcttctg acgcaagcag ccgcggcgcg cggggcttga gcggcgcgat
    24541 cgtgcgccgc gcccgcaggt agcgcgccgc cagctcccat accggctcgc cgctcgaacc
    24601 ttccgcgacc ggcgcccagc ccgccacctt gtagcgcctg cccgcctcca gcggccggcc
    24661 gccgaggcgc agatccgaga tgcgcgcgcc gacgcccgcg cgcggccggc acgcgtagct
    24721 caggccgccg acgcgcacca tgtcgccgcc ctgctgcagg tagggatccg ggttgaaggt
    24781 gttgtccgcg acgtcctcga ggatcgcctt gatctgcgcg ccggtgagtt cattcagggt
    24841 ggcgtaggga taggtgatgg cggtctggtc catcaggtct tcgaacgtga tcgcgtcccc
    24901 ggggagcaag gtgccgcccc agcgaaagcc cggcgagaag gcgatctcgg cgtccttctc
    24961 cttgagcagc gcttcgagca gcagctcgtc ccagctgccg ttgtaggtgc cgcggcgata
    25021 cagcagcccc tcggtggcgg cgagcttctc ctccagctgc gcgcggtagg gcgcgcgcac
    25081 cttgtcgatg taggacgcca tcgccgcgtc cgccgggagg aggttcgcga acaccggcag
    25141 cagccggtag cggtagcccg tcaccgcgcc gccttgcacc tcgaggtcga gcaggccgag
    25201 gaacttgccg tgcgaaccgg cgttcgtcac cagcgtgcgt cccacgcgca ccggcttcgc
    25261 gatgccgtcg tgcgtatggc cgccgaggat ggcgtcgatg ccgcgcacgc gcgcggcgag
    25321 cttcagatcc acctgcaggc cgttgtgcga caggagcacc accgcctgcg cgcccttggc
    25381 gcgcacttcg tcgaccagct gctgcaggcg cggttcctgg atgccgaagg tccactcggc
    25441 gaccaggtgg cgcgggttgg cgatcggcgt gtaggggaag gcctggccga tcaccgccag
    25501 cgccacgccg ttcagcgagc gcaggctgta cggcttgaac accgggtcct cgaaatcggc
    25561 ggtcttcacg ttctgcgcga ggaactcgat cggcgccagc tcgcggtcca ccgcctcgcg
    25621 cacgcgcttc gcgccgtagg tgaactccca gtgcgcggtc atcaggtcca cgccgagcag
    25681 cttctgcgcg ccgatcatgt cggcgccccc ggtccacagc gcggtggccg agccctgcca
    25741 ggaatcgccg ccgtccagca gcagcgcgtg cgggcgcgag gcgcgcaggc gcttcaccag
    25801 ggtggcgagg tgcgcgtagc cgccgacctt gccgtagcgg cgcgcggcgc gctcgaaatc
    25861 cacgcggctg tgggcatagg ccgcggcgct gccggcggcg atgccctggc gctcgaggaa
    25921 ggccgcgccg gccggctcgc ggaagtacag cggcaggagc tgcgcgtgca cgtcggtgag
    25981 gtgcaggagc gaaaccttgc cgtagggagg cggctggtag agctcgtcgg ccgcctgcgc
    26041 cgcgcccagg cccgggcgca gcgaggcccc ggcagctgcg gcgaacgaga gccaccggag
    26101 gaactcgcgc ctgctattgt tatggggcat ggcggcgccg agcataacaa acgtcaggac
    26161 caccacctgc tacatgtgcg cttgccgctg cggcatccgc gtgcacctgc gcgacgggaa
    26221 gatccgccac atcgacggca acccggcgca cccgctgaac aagggcgtga tctgcgccaa
    26281 gggcgccgcc ggcgccatga aacaggactc gccggcgcgc ctcacgcggc cgctgcggcg
    26341 caaggcgggc gcggcgcgcg gcgccggcga gttcgagccg atctcctggg acgaggccta
    26401 cgcgctgctc gaagagcgcc tcgggcgcat ccgcgccacc gacccgaaga agttcgcttt
    26461 cttcaccggc cgcgaccaga tgcaggcgct caccggcctc ttcgcgcgcc agttcggcac
    26521 gccgaactac gcggcgcacg gcgggttctg ctcggtgaac atggccgcgg gcatgatcta
    26581 caccatcggc ggctcgttct gggagttcgg cgggccggac ctcgagcgcg cgaagctgtt
    26641 cgtgatgctc ggcaccgccg aggaccacca ttccaatccg ctgaagatcg cgatcgcgaa
    26701 attcaagcgc gacggcggcc gcttcatctc ggtcaatccg gtgcgcacgg gctactccgc
    26761 gatcgccgac gagtggatcc cgatccggcc cggcaccgac ggcgcgctgc tgatggcgct
    26821 gtgccacgag ctggtgcgca ccgggcgcac cgacgcggaa ttcctcgccc agtacaccaa
    26881 cgccgggtac ctcgtgcgcg aggacgacgg ccgcatcgcc tgcgcgggcg gcagcccgca
    26941 ggacaacccg ttccgccgcc acgaccagct gtggtgggac aaggcgagcg gcgcgccggt
    27001 ggcgaaccac gccgcgggcg ccgagcccgc gctggacggc agctaccacc tcgccgacgg
    27061 cacgccggtg cggccggcct tcgcgctgtt gcgcgagcgt ctcgccggct gcacgccaga
    27121 gtgggccgcg gcgatcaccg gcatcgacgc ggcgaagatc cgcgcgctcg ccgaggagat
    27181 gggccgcacc gcgctcgagc aaagcttcga gctgccgatc gcctggaccg acagctgggg
    27241 gcgcaggcac gggtcggtgc gcggcaaccc ggtcgccttc cacgccatgc gcgggctcgc
    27301 cgcgcattcc aacggctttc acgccgtgcg cgcgctctcc atcctgatga cgctgctcgg
    27361 caccatcgac cggccgggcg gattccgcca caaggcgccg ttcccgcgca gcacgccccc
    27421 ggtgtacgcg cggctgccgc aggccggcgg cgcggtgcag cccgggctgc cgctggcggg
    27481 cgcggcgctc gccttccccg ccgggcccga cgagctgcag gtggacgacg cgggccggcc
    27541 gctgcgcatc gacaaggctt tttcctggga gcacccgctg gcggtgcacg gcatgatgca
    27601 caacgtcatc gccaacgcct ggcgcggcga tccctacccg atcgacacgc tgctgatctt
    27661 catggcgaac atggcgtgga actcctcgat gaacacgccc gaggcgcgcc gcatgctgaa
    27721 cgacaaggac gcctcgggcg agtacaagat tccgttcctc gtggtctgcg acgccttcca
    27781 gtccgagacg acggcgtacg cggacctggt gctgcccgac accacctatc tcgagcgcca
    27841 cgacgtcatg tcgctgctgg accggccgat ctcggagttc gacggcccgg tcgactcggt
    27901 gcgcgtgccg gtcgtgccgc cgacgggcga gtgccggccg ttccaggacg tgctggtcga
    27961 gctcgccggg cgcctcaagc tgccggcgtt caccaacgcc gacggcacgc gcaagttccg
    28021 cgactacgcg gatttcatcg tcaacttcga gaccgagccg ggctcggggc agggcttcct
    28081 catcggctgg cgcggcaagg acggggacca ggcgctcctc ggcgcgccca acccgcgcca
    28141 gtgggagcgc tacgccgaga acaactgcgt gttccactac gccatgccgc cggagcacca
    28201 gtacatgcgc aactggaacc gcggctacaa cgaatgggcg gtgaagatga aactgcgccg
    28261 ccacccggac ccggtggtga tcgcgatcta cgccgaggtg ctgcagggct ttcgcctcgc
    28321 cgcgcagggt cgcgggcccg cgaagcgccg cccgcccgag gcctggcgcg cgcgcgtgga
    28381 gaagtatttc gacccgctgc ctttttacta tgcgccgctc gaggcgcagg ccgtcgacgc
    28441 cgccgcctac ccgctcgcgg cgatcacgca gcggccgatg gccatgtacc actcctggga
    28501 ttcgcagaac gcctggctgc ggcagatcca cgcgcacaac tacctgttcg tcaatccgcg
    28561 caccgcgcgc gcggcgggcg tcgccgacgg cggctggatg aagctcgcgt cgccctgggg
    28621 cgaggtgcgc tgcatggcgc gccactcgga agcggtggag ccgggcacgg tgtggacgtg
    28681 gaacgccatc ggcaagtcgc cgggcgcctg gggcctcgcg cccggcgcca acgagttcac
    28741 gcgcggcttc atcctgaacc acgtcatcgc ggagtccctg ccggacggca cgtcgaattc
    28801 tgacccggtg accgggcagg cgggctggta cgacgtgcgc gtgaggattt cgcccgcatg
    28861 accaccaaac aactcgcgct ggtcatcgac ctcaacgtct gcgtgggctg ccacgcctgc
    28921 gtgaccagct gcaagtcctg gaacaacgcc ggcgcggccg ggccgctcac cgaccagaat
    28981 ccctacggcg cggacccgag cggcaccttc ttcaaccgcg tgcagacctt cgaggcgggg
    29041 gagttccccg ccaccgagac ggtgcacttc ccgaaatcct gcctgcactg cgaggacccg
    29101 ccctgcgtcc cggtgtgccc cacgggcgcc tcgtacaagc gcgccgagga cggcatcgtg
    29161 ctggtggact acgacaagtg catcgggtgc aagtactgcg cctgggcctg cccctacggc
    29221 gcgcgcgaaa tcgacgagca gcagcaggtg atgaagaagt gcacgctgtg cgtggaccgc
    29281 atctacgacg agaagctgcc cgcggcggac aggaagcccg cctgcgtgct cgcctgcccg
    29341 accagcgcgc gcatcttcgg cgacgtgcac gacccggaat cggaagcctc gcgcgccatc
    29401 cgcgagcgcg gcggctacgc gctgatgccg gaatgggaga ccaggccggc gaaccactac
    29461 ctgccgcgca ggaaatgagg cccgcctggt cggtgctcct gttcaccacg ctcgccgggg
    29521 cggggcaggg attgttcctc gcgctgttcg ccgcgcagcc gcagggccgg ctgatgcagg
    29581 cgggccttgc gacctcgctc gcgctcctcg ccgcgggcct catcgcctcg ttcttccacc
    29641 tcgggcaccc ggagcgcgcc tggcgcgcgg ctgccatgtg gcgcacttcc tggctgtcgc
    29701 gcgaagtgat cgtgctgccc gcgttcatgg ccctggtggc cgcctatggc tggacgcaga
    29761 gcgcgctcct cggcgcgctg gcggtcgcgg cctgcctcgc gctgttcgcc tgcaccggga
    29821 tgatctacgc ctgcctgccg ttcctgcagg agtggcgctc gccgctcacg ctggtcaatt
    29881 tcacgctgct cggctgcgcc tcggggctca ccctcgccgc ggcgctcgcc gccgcgctcg
    29941 agccggcgct cgcgcgcggc tacgtgctcg ccgcgctcgc cgtcggcgcc gcggcctacc
    30001 tcggcaggct cgcctcgctc tttcgcaatg cgcggctgcg gccgaagtcc acgctcgcca
    30061 gcgccaccgg catcccgcag cggccgatcg tgcagatgtc gcagggcgcg atgggcggct
    30121 cgttcaacac gcgcgagttc ttccacggcc ggccgcgcgc cgtggtgcgc gcggcgcgct
    30181 ggaccctgct cgcgctggtg ttcccggtgc cggcggcgct gctgctcgcc gggcaggcgg
    30241 cgccggcgtt cctgctgcag ttcgcggggc tgctcgccga gcgctggtac ttcttcgccg
    30301 aggcgaagca cccgcagaat ctttactacc aggccatagc ctgagaacat ccgcgcatgc
    30361 accacgacgt cgagcttgac gcccgcggcc tgaactgccc gctgccgatc ctgcgcgcga
    30421 agaaggcgct gaacggcatg caatcggggc aggtgctcaa ggtggtggcc accgaccccg
    30481 gcgcggtgaa ggatttccag accttcgcca agcagaccgg caacgagctg ctgcagcacg
    30541 cggaggccga ccgggagttc acgctctaca tgaagcgcaa gtgaacgcgg cctgggtgac
    30601 gggcggcgcc ttcgcgctcg ctttcgtgtt cggcgcggtg ggcaaccgca ccaacttctg
    30661 caccatgggc gcggtgtccg actgggtcaa catgggcgac ctgaaccgca tgcgcatgtg
    30721 gctgctcgcc atcgcggtgg cgctcgccgg ctcgagcgcg ctgcacctcg ccggcatcat
    30781 cgatctttcc aagtcgatct atcccggtcc caacttcacc tggctctcct acctcgtcgg
    30841 cggattcctc ttcggcgtcg gcatgacgct gggctcgggc tgcggctcga aaacgctgat
    30901 ccgcatcggc gcgggaaacc tgaagtcgct catcgtctac gtgttcctcg gcatcggcgc
    30961 ctacatgacg ctgaagggcc tgttcggcgc cttccgcgtc ggcgtgctgg agaaagccgc
    31021 ggtgacgctc cccgccgggc aggacctgcc cgcgctcctc ggcctgcacc gggcggtgcc
    31081 cgcggcgctg atcggcggcg ccctgctcgc cttcgtctac cgcagccggg agttccgcgc
    31141 cggcttcgac tacacgctgg gcggcgtggt gacgggtctt gtggtggcgg gcggctgggt
    31201 ggtgagcggc gtgctggggc acgtggcgga gcacccggag acgctgcagg aggcgttcat
    31261 cgccaccaat accggccgca tggagtcgtt ctccttcgtc tcgccgatgg cctacacgct
    31321 cgaatacctg atgctgtgga ccgacaagtc gaagatcgtc acctacggca tcgcctcggg
    31381 cctcggcgtg atcgcgggct cggccgccta cgcgctcgcc acgcgcagct tccgctggga
    31441 gggattccgc gacgccgagg acaccgcgaa ccacatggtc ggcgggatgc tgatgggctt
    31501 cggcggcatc acggcgctgg gctgcaccat cggccaggcg gtcaccggga tctcgacgct
    31561 cgcgctgggc tcgttcatga ccttcgccgc catcgtcgcc ggctcggcgc tcaccatgaa
    31621 ataccagtac tggcgcgccg gggcttgagt tgaaacgcgt tcttgccctc tggctgggct
    31681 gcgccacgct cgccgcggcc gccgacgcga cgcacacggt gaaactgctc acgccggaga
    31741 cggcgctcaa ggcggccctc gccgcgcaga agaaatgccg cgacggcggc caccaggcga
    31801 cggtggcggt ggtggaccgc atgggcgtga cgcaggtgct gctgcgcgac cggtttgccg
    31861 ggccgcacac cgtggacacg gcgcgcgcca aggcctggac cgcggcgagc tttcgcaccg
    31921 acaccaccgg gctcgccgag gcgagccagc ccggcaggcc ggcgagcggc atccgcaacc
    31981 tgccgggcgt ggcggcggtg ggcggcgggc tgatgatcca ggcgggcggg tcgctgctcg
    32041 gggcgatcgg cgtctcgggc gcgccgggcg gcagggagga cgacgcctgc gccgcggcgg
    32101 ggatcgcggc gatccgcgac gagctcgaat tgtgaaaggc gggcgtcggc cgcgctaaaa
    32161 tccggctccg gttccgtttc tttctccaat cctcacgaga ccagcccgat ggaccaggcc
    32221 ctgctttcca agaccgccgc cgcgatggtg gcgcgcggca aaggcgtgct cgccgccgac
    32281 gagtcgagcg ccacctgcga gaagcgcttc aagtcggtgg gcgccgattg caccgaggag
    32341 agccgccgct tctaccgcgg cctgctgttc accgcgccgg gcgtcgagca gttcctctcc
    32401 ggcgtgatcc tgttcgacga gacgctgcgc cagaagacgg acgacggcgt gccgttcccg
    32461 gagtacatgg cgaagaaagg catcctgccc ggcatcaagg tggacaaggg cgccatggac
    32521 ctggcgctgt gccccggcga aaaggtgacc gagggcctcg acggcctgcg cgcgcggctg
    32581 gcggaatact tcaagctcgg cgcgcgcttc gccaagtggc gcgcggtgat caccatcggc
    32641 gacgaaggtt ccacgctccc ctccgctgcc tgcatcgagg ccaacgcgca tgccctggcg
    32701 cgctacgcgg cgctgtgcca ggaggcctcc atcgtgccga tgatcgagcc cgaggtgctg
    32761 ctcgacggcg gccacacggt ggagcgctgc gaggaagtga ccgaggccac gctgcgcgcc
    32821 acctacgcgg cgatggctgc gcagtcggtc gccgtggaac agctgatcct gaagaccagc
    32881 atggtggtct cgggcaagga ctgcgcgcgc caggcaggcg tggacgaagt cgccgagcgc
    32941 acgctgcgcg ttctgcggcg caccgtgccc gcggcgcagc cgggcatcgt gttcctctcc
    33001 ggcggccagt cggacgaggc ggccaccgcg cacttgaacg cgatggcctc ggcgccgggc
    33061 ctgccctggc cgctgacctt ctcctactcg cgcgcgctgc agaacccggc gctcaaggcc
    33121 tggcgcggcc aggcggccaa cgccgccgcg gcgcagaagg ccttctgcca ccgcgcccgc
    33181 atgaacggcc ttgcggccca gggcaagtgg caggccgcgc tggaaaagca ggccgcgtga
    33241 tgaaaatagg gtcagacccc attttcgaat ccagcctgaa aagcctgccg cgcatcggca
    33301 agggcaaggt gcgcgacatc tacgccgtgg gcgaggacaa gatgctgatc gtggcgagcg
    33361 accggctctc ggccttcgac gtggtgctgc ccgacccgat cccgcaaaaa gggcgcgtgc
    33421 tcaacgaaat ggcgaacttc tggttcggca agctcggcca catcgtcccg aaccagctca
    33481 ccggcatcga cccggaagag gtggtcgagg gggaggacga gaaggcgcag gtgcgcggcc
    33541 gctcggtggt ggtgaagaag ctgcggcccc tgccgatcga ggcggtggtg cgcggctacc
    33601 tcatcggctc tggctggaag gactaccaga ggaccggcaa ggtctgcggc atcgagctgc
    33661 cgaagggcct gcagcaggcg cagaagctgc cgcagcccat cttcaccccg gccaccaagg
    33721 cggaagaggg ccacgacgag aacatctcct tcgacgaagt ggagcagctc atcggcgaga
    33781 agctcgccaa ccgggtgcgc gaaatctcgc tgcgcctgta caaggaggcc tcggagtacg
    33841 cggcgacccg gggcatcatc atcgccgaca ccaagttcga gttcggcacc gacgacaagg
    33901 gcggcctggt cctcatcgac gaggtgctga ctgcggactc ctcgcgcttc tggccggcgg
    33961 attcctacca ggtgggcatc agcccgccgt cgttcgacaa gcagttcgtg cgcgactacc
    34021 tggagaccat gcagtggaac aaggcgccgc cggcgccgcc gctgccgcgc gaggtgatcg
    34081 ccaagacctc ggaaaaatac caggaggcgc tcgagcgcct caccgggagg aagctcgcat
    34141 gatcggcatc gtgatggggt cgacctccga ctgggagacg atgaaggctg cggcgaagat
    34201 gctcaaggaa ttcggcgtcg ccttcgaggc gaaggccatg tccgcgcacc gcagcccgca
    34261 cgcggtggtg aaatggaccg agggcgcgga aaagcgcggg ctgcgggcga tcatcgccgc
    34321 ggcgggcggc gcggcccacc tggcaggggt ggtggcggcg cacaccacgc tgccggtgct
    34381 cggcgtgccg atgccctcca agcacctcaa cgggctggac tcgctgctct ccacggtgca
    34441 gatgcccaag ggcgtgccgg tggccacctt cgccatcggc gaggcgggcg cggcgaacgc
    34501 ggcgctgttc gcggtggcgc tgctcgcgct gtcggacaag gcgctggcga agaagctgtg
    34561 gaagttccgc cgctaccaga ccgcggcagt cctgaaagct gcgccgctca agctgtgagg
    34621 cccgaagtcc ggcgcgccgc ggagatcctc gagcgcggcg ggctggtcgc gtttcccacc
    34681 gagaccgtgt acgggctcgg cgcggatgcc tcgagcaggg aagcggtggc gcggctgtat
    34741 gccgccaagc gccggccggc ggaccatccg gtgatcgtgc atttcgccga tgcgcaaggc
    34801 gccttcgcct gggcgagcgc agtaccgccg gcggcgatga agctcgcggc gcagttctgg
    34861 cccgggccgc tcactctcat cctgaagagg tccgcgcagg cgggcgattt cgtcaccggc
    34921 ggccaggaca cgatcggcct gcgcgtgccg tcgcatccgg tggcgcagga gctgctgcgc
    34981 gccttcggcg gcggcatcgc cgcgccctcg gcgaaccgct acggcaaggt ttcgcccact
    35041 accgcggcgc atgtgcgcga ggacctggga gaagacgtgg cgctggtgct cgacggcggg
    35101 ccgagcgagg tggggatcga gtccgccatc gtggatctct cgggcggccg ggcggtgctg
    35161 ctgcgcccgg gcgggatctc gagacagcag ctggcgcagg cgctcggcga aaccgtcgag
    35221 gaaaagcgcg ccgactcgcc gcgccattcg ggcgggcacg agcgccatta cgcgccgcgg
    35281 acgccggcgc gcctggtgcc cacgcacacg ctcgaccagg agattgcgaa gctgggcagc
    35341 aaggtggcgg tgctcgcctt ctcgcggccc gacgagcggg tggactactg gctgcgcatg
    35401 ccgcgggagc ctgcgggcta tgcgcagaag ctctacgccg cgctgcgcga gttggacggg
    35461 gcgcaatgcg agagtatcct catcgaggcg ccgcccgaga ccgcggaatg ggccggggtg
    35521 cgcgaccggc tgctgcgcgc gacttcatga gaatccttct ccttgtttca ttgctgatct
    35581 ccggctgctc gcacaccagc gtgcagggcg gctggggcgg cgcgggcccg tcgggcggca
    35641 acgtgtcggt gggcgtgagc ggcagcgcgg tgggctgggc gctgatcggc atcggcctca
    35701 tcgccgcggg ggagtatgcg gagcagcggc gcctggacgg cctgccgccg atgaacgagc
    35761 cgccgcggga gctggacaag ggccggcgcg tgaacgagca ggattgctcg cagccgatca
    35821 aggactggtc ggccaacctg aagtgccggt aggcggtttt cccgagcagc gctatctcgt
    35881 cggcgcgcgc gagctgcgcg agcgcctggg cgatgtgacg ctgctggacc tgcgtcctgc
    35941 agaggatttc gccgtggggc gcatcgaggg cgcccggcat ctcgatttgt acggcgtcag
    36001 cctcaatgat tcgtccgaag cgccgctcga agcgttcctc gccatcttcc gcgtgctgtt
    36061 cggggcgcgc ggcgtgactc tggacaagcc ggtggtggtg tacgaccacg agagcggcga
    36121 gcgcgcgtcg cgcgcggtgt ggctgctgac ggtgctgggg catccggatg cgcggctgct
    36181 cgatggcggg acgcaaggct gggtggcggc gggcgggaag cttgcgcgcg tggatgctgc
    36241 gccgccgccg gtggatccaa ccaaggcgcc gccgaccgcg ccgccgttca agggcaccat
    36301 gaacatggat ctggtcgcga gccggttcga cgtgcagcgc gcgatcggga atcttgagta
    36361 cgtgatcgtg gatgtgaggc gcgggtccga gtaccggggc acggagaaac gcgcgcggcg
    36421 cgcgggaacc attccgggcg cggtgcacgt gttctggcgc gatcacctgg atgcaaaggg
    36481 cgcgctgcgt ccggcggagg aggtacgcgc gctgtatgcg tcacgcggcg tgacgccgga
    36541 caagaacgtg atc
//
LOCUS       DASRIE010000071         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2152280, whole genome shotgun sequence.
ACCESSION   DASRIE010000071 DASRIE010000000
VERSION     DASRIE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152280"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFB53_03425"
     CDS             complement(<1..353)
                     /locus_tag="VFB53_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85815.1"
                     /translation="MSRYDAVVVGAGHNGLTCACYLARGGLKVKVLERRPRVGGAAVT
                     EEFHPGFRNSAAAYTVSLLQAKVIRDLGLAGHGLRIVERPLANFLPLEGGYLKVGGGL
                     EATQRAFAKFSARDA"
     gene            complement(358..2175)
                     /gene="glmS"
                     /locus_tag="VFB53_03430"
     CDS             complement(358..2175)
                     /gene="glmS"
                     /locus_tag="VFB53_03430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZN85816.1"
                     /translation="MCGIVGAAARRDIVPVLIEGLRRLEYRGYDSCGVAVLGAGTLER
                     VRSVARVEHLAGQVRRQKLRAETGISHTRWATHGAPVTRNAHPMVSRGEIALVHNGII
                     ENYEELRAELKAQGYAFDSQTDTEVVTHLVHSCYEGDLFRAVRRAVKRLAGAYAIAVV
                     ARSQPHRVVGARAGSPLAIGVGKGEMFLASDALALAGTTDRIAYLDEGDVAEIDLEGF
                     RVRDAADREARREVRTVKSAAGAVELGPYRHYMQKEIFEQPSAVADTLEGVQGAAPAL
                     FGKQAAGTLRGVKQVLVLACGTSYYSGLTAKFWIESLAGLPAQVEIASEYRYRDSVPD
                     PATLVVAISQSGETADTLAALRHARKLGQKRTLAICNVATSAMVRETRLKYLTRAGTE
                     IGVASTKAFTTQLTALFLLALVLAKLRRRLPARDEARHLKRLRHLPKAIAAALALEPQ
                     VIAWSERFAKKEHALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVT
                     EDMPVVTVAPNDALLEKLKSNMQEVRARGGELYVLADADTRIAAQEGMHVIRLPEHYG
                     PLSPILHVVPLQLLAYHTALARGTDVDNPRNLAKSVTVE"
     gene            complement(2179..3534)
                     /gene="glmU"
                     /locus_tag="VFB53_03435"
     CDS             complement(2179..3534)
                     /gene="glmU"
                     /locus_tag="VFB53_03435"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZN85817.1"
                     /translation="MGLAVIVLAAGQGKRMHSALPKVLHALAGRPLLAHVLDAARALQ
                     PSQILVVHGHGGADVRAAFAGARIEWVEQSQQLGTGHALAQALPKVARGAFVLVLNGD
                     VPLARAATLERLVEAAKGGLAICTSELSDPSGYGRVLRDAKKRVRRIVEHKDASAKEL
                     RIREWYAGFLAAPAERLSRWMAGVKPANAQGEYYLTDVIALAAKDGVNVATVKAADPW
                     EVAGVNSRQELAMLERACQNAQARKLLEAGVGLADPSRVDVRGELSCGRDVHIDVNCV
                     FEGSVTLGDRVRIGPNCVLRNVGVDADTQVLAFSLLEDAEVGKRCRVGPYARLRPGAR
                     LAEEVHVGNFVEVKASRIGAGSKANHLAYIGDAEVGARVNIGAGTITCNYDGSAKHRT
                     VIEDDCFIGSDATLVAPVTIARGSYIGAGSTISKSTPPGQLTVARARQVSLANWKPKK
                     K"
     gene            3616..4320
                     /locus_tag="VFB53_03440"
     CDS             3616..4320
                     /locus_tag="VFB53_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZN85818.1"
                     /translation="MAIALHSEYLEISFRLVAALAAGGIIGLERSFRGRAAGFRTHAL
                     VCIASSLLMLVTVYEHQWFVAQSGSRVVIDPTRMAQGIMTGIGFLGAGVIVKEGLTVR
                     GLTTAASIWITSAIGILTGIGFYFAAGLGVVLTLGTLSVFRWIEGKMPTELYANFTVR
                     FARDAVMPEALLRDLVRQHGFSVANLNYRLIGETGQFEYRMVMRTLKTDNVRRLSETL
                     NADRAVAEYRISPASD"
BASE COUNT          611 a         1573 c         1472 g          686 t
ORIGIN      
        1 tgcgcgtcgc gggcggagaa tttcgcaaac gcgcgctggg tcgcctccag cccgcccccc
       61 accttcaggt agccgccctc gagcggcagg aaattcgcca gcggccgctc gacgatgcgc
      121 aggccgtgcc cggcgagccc caggtcgcgg atcaccttcg cctgcagcag gctcaccgta
      181 taggcggcgg ccgaattgcg gaagcccggg tggaattcct cggtgaccgc ggcaccgccg
      241 acgcgcggcc ggcgctccag caccttgacc ttgagcccgc cgcgcgcgag atagcaggcg
      301 caggtaaggc cgttgtgccc ggcgccgacg accaccgcgt cgtagcggct cacggcgtta
      361 ttcgacggtg accgacttcg ccaggttgcg cgggttgtcc acgtcggtgc cgcgcgcgag
      421 cgccgtgtgg taggcgagca gctgcagcgg caccacgtgc aggatcggcg acagcggccc
      481 gtagtgctcg ggcaggcgga tgacgtgcat gccttcctgc gcggcgatgc gcgtgtcggc
      541 gtccgcgagc acatacagct cgccgccgcg cgcacgcact tcctgcatgt tcgacttgag
      601 cttttcgagc agcgcgtcgt tcggcgccac cgtcaccacc ggcatgtcct cggtcaccag
      661 cgcgagcggg ccgtgcttga gctcgcccgc cgggtaggcc tcggcgtgga tgtaggaaat
      721 ctccttgagt ttcagcgccc cttccagcgc gatcgggtaa tgcaggccgc ggccgaggaa
      781 cagggcgtgc tccttcttgg cgaagcgctc cgaccaggcg atgacctgcg gctccagcgc
      841 gagcgccgcg gcgatcgcct tgggcaggtg gcgcaggcgc ttcaggtgcc gcgcttcgtc
      901 gcgcgccggc agccgccggc gcagcttggc cagcaccagt gcgagcagga acagcgcggt
      961 gagctgcgtg gtgaaggcct tggtggaggc gacgccgatc tcggtgcccg cgcgggtgag
     1021 gtacttcagg cgcgtctcgc gcaccatggc gctggtggcg acgttgcaga tggcgagggt
     1081 gcgcttctgg cccagcttgc gcgcgtggcg cagcgcggca agcgtgtcgg cggtttcgcc
     1141 cgactgcgag atcgcgacca ccagcgtggc cgggtccggg acgctgtcgc ggtagcggta
     1201 ctcgcttgcg atctccacct gcgccggcag gccggccagc gattcgatcc agaacttggc
     1261 ggtgagcccc gagtagtagc tggtgccgca agcgaggacc agaacctgct tcacgccgcg
     1321 cagcgtgccc gccgcctgct tgccgaacag cgccggggcc gcgccctgca cgccttccag
     1381 cgtatcggcc accgcgctgg gctgctcgaa gatctccttc tgcatgtagt gccggtaggg
     1441 tcccagctcg acggcgcccg ccgcggactt gactgtgcgc acctcgcgcc tggcttcccg
     1501 gtccgccgca tcgcgcacgc ggaaaccctc gaggtcgatc tccgcgacgt cgccctcgtc
     1561 caggtaggcg atgcggtcgg tggtgccggc gagcgccagc gcatccgagg caaggaacat
     1621 ctcccccttg cccacgccga tggcgagcgg gctgcccgcg cgcgcgccga ccacccggtg
     1681 cggctggctc ctcgccacca cggcgatggc ataggcgccc gccaggcgct tcacggcgcg
     1741 gcgcaccgcg cggaacaggt cgccctcgta gcagctgtgc acgaggtgcg tcaccacctc
     1801 ggtatcggtc tggctgtcga acgcgtagcc ctgcgccttc agctcggcgc gcagctcctc
     1861 gtagttctcg atgatgccgt tgtgcaccag cgcgatctcg ccgcgcgaga ccatcgggtg
     1921 ggcattgcgc gtcaccggcg ccccgtgcgt ggcccagcgc gtgtgcgaaa tgccggtctc
     1981 cgcgcgcagc ttctgcctgc gcacctgccc ggcgaggtgt tccacgcgcg ccacgctgcg
     2041 cacgcgctcg agcgtgcccg cgcccagcac cgccacgccg caggagtcgt agccgcggta
     2101 ctccagccgc cgcaggccct cgatcagcac cggcacgata tcgcgccgcg cggcggcgcc
     2161 gacgattccg cacatggcct atttcttctt cggcttccag ttggcgaggg acacctgcct
     2221 ggcgcgcgcc accgtgagct gcccgggggg cgtgctcttc gagatggtgg agccggcgcc
     2281 gatgtaggag cccctggcga tcgtcaccgg cgccaccagc gtcgcatccg agccgatgaa
     2341 gcagtcgtcc tcgatcacgg tgcggtgctt ggccgacccg tcgtagttgc aggtgatggt
     2401 gccggcgccg atgttgacgc gcgcgcccac ctcggcgtcg ccgatatacg ccaggtggtt
     2461 cgccttggac ccggcgccga tgcggctcgc cttcacctcg acgaaattgc cgacgtgcac
     2521 ctcctcggcg aggcgcgcgc ccgggcgcag gcgcgcatag ggtcccacgc ggcagcgctt
     2581 gccgacctcg gcgtcctcga gcagcgagaa cgccagcacc tgcgtatccg catcgacccc
     2641 gacattgcgc aggacgcagt tcggcccgat gcgcacccgg tcccccagcg tcacgctgcc
     2701 ctcgaacacg caattcacgt cgatgtgcac gtcgcgcccg caggagagct cgccgcgcac
     2761 gtcgacgcgc gacgggtcgg cgagaccgac gccggcctcg agcagctttc ttgcctgcgc
     2821 gttctggcag gcgcgttcca gcatcgcgag ctcctgccgc gaattgacgc ccgccacttc
     2881 ccagggatcc gccgccttca ccgtggcgac gttgacgccg tccttcgctg ccagcgcaat
     2941 cacgtcggtg aggtagtact ccccctgggc gttcgcgggt ttcaccccgg ccatccatcg
     3001 cgacagccgt tccgccggcg cggcgagaaa acccgcgtac cactcgcgga tgcgcagctc
     3061 cttcgcgctc gcgtccttgt gctcgacgat gcgccgcacg cgcttcttcg cgtcgcgcag
     3121 cacgcggccg tagcccgacg ggtcgctcag ctcggaagtg cagatggcaa gcccgccctt
     3181 cgccgcctcc accagccgct ccagcgtcgc cgcgcgcgcc agcggcacgt cgccgttcag
     3241 caccagcacg aaggcgccgc gcgccacctt cggcagcgcc tgcgccaggg cgtgtccggt
     3301 gccgagctgc tgcgactgtt ccacccactc gatgcgcgcg cccgcgaatg ccgcgcgcac
     3361 gtcggcgccg ccatgcccgt gcacgacgag gatctgcgag ggctgcagcg cgcgcgccgc
     3421 gtcgagcacg tgcgccagca acggacgccc ggcgagggca tgcagcacct tgggcagcgc
     3481 cgaatgcatg cgctttccct gcccggcggc gagcacgatc acggcgaggc ccatggcggc
     3541 ggattctagc atccgcatta tggcgaactc cttgaaagat catggaaaag ccacgtacta
     3601 tgacagtccg acgccatggc gatagctctc cacagcgaat acctcgagat cagtttccgg
     3661 ctggtcgccg cgctggccgc cggcgggatc atcgggctgg aacgcagctt ccgcgggcgc
     3721 gccgcgggct ttcgcacgca cgcgctcgtc tgcatcgcct cgagcctgct gatgctggtc
     3781 acggtgtacg agcaccagtg gttcgtcgcg cagagcggct cgcgggtggt gatcgatccc
     3841 acgcgaatgg cgcagggcat catgacgggc atcggcttcc tcggcgccgg cgtgatcgtc
     3901 aaggagggtc tgacggtgcg cgggctgacc accgcggcgt cgatctggat cacctccgcc
     3961 atcggcatcc tcaccgggat cggtttttac ttcgccgcgg ggctgggcgt ggtgctgacg
     4021 ctcggcacgc tgtcggtgtt ccgctggatc gagggcaaga tgcccaccga gctctacgcc
     4081 aacttcacgg tgcgcttcgc ccgcgacgcg gtgatgccgg aggccctgct gcgcgatctg
     4141 gtcaggcagc acggcttttc ggtggccaac ctgaattacc gcctgatcgg cgaaaccggg
     4201 cagttcgaat accgcatggt gatgcgcacc ttgaagaccg acaacgtgcg caggctttcg
     4261 gagacgctca acgccgaccg ggcggtcgcc gaataccgca tttcccccgc cagcgactga
     4321 gtcagcgcgg caggtcgctc tt
//
LOCUS       DASRIE010000072        11287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2696192, whole genome shotgun sequence.
ACCESSION   DASRIE010000072 DASRIE010000000
VERSION     DASRIE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696192"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFB53_03445"
     CDS             complement(<1..766)
                     /locus_tag="VFB53_03445"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZN85819.1"
                     /translation="MSRAADARLLLERIATAYAPAALSSSLGAEDMVLTDLIAREGLP
                     IEIFTLDTGRLHAETRDMLGRVQSHYGIALRVMQPEAQAVVEYVTTHGRNAFYGSVEL
                     RKRCCEIRKVEPLRRALAGKRAWITGLRRDQSAGRAGLRLAEFDAALHLAKFNPLAEW
                     SGNDVWEYVRAHKVPVNPLHERGYPSIGCEPCTRAVAPGEDERAGRWWWEEEGKKECG
                     LHVAGPAAAGASAVAFKNDLRFFGGEPHAAKKPETME"
     gene            complement(763..1155)
                     /locus_tag="VFB53_03450"
     CDS             complement(763..1155)
                     /locus_tag="VFB53_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZN85820.1"
                     /translation="MATLIRRGKIAHPSPPEAGEVLRLEPADDPAGVADRLGAAARVE
                     VNFPRFNDGRGYSIARLLRERHGYKGELRAVGDVQRDQIFYLSRCGFDAFLLREGEDA
                     EAALAALRDFSEAYQASVERPQPLFRRR"
     gene            complement(1106..2812)
                     /locus_tag="VFB53_03455"
     CDS             complement(1106..2812)
                     /locus_tag="VFB53_03455"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZN85821.1"
                     /translation="MYLYDEIDQKIVDERVAQFRDQTRRFLEGKLKEDDFRPLRLQNG
                     LYVQRYAPMLRVAVPYGILSSRQLRMLAHIARKYDRGYGHFSTRQNIQFNWPKLEQVP
                     DILADLASVQMHAIQTSGNCVRNITADHFGGVARDEIVDSFVWCEVLRQWSTFHPEFA
                     YLPRKFKIAVNGAEADRAATFLHDIGLHAVRDAAGAVGFRVIVGGGMGRTPLIGHAIR
                     EFLPWQHLLSYVEAILRVYNRYGRRDNMHKARIKILVKERGPEKFRDEVEAEWAQLKD
                     GPSTLTEAELRRVAGRFTRPRYQPLEDLRLAQEGPFGAWLKRNVFPHKQPGYAAVTLS
                     LKRTGVPPGDATDAQMDAVADLAERYSFGELRVTHEQNLVLADVRQDQLEVLWREARE
                     LGLATPNIGLLTNIIACPGGDFCSLANAKSIPVAEAIQRLFDDLDYLHDIGELDLNIS
                     GCINSCGHHHVGHIGVLGVDKQGEEFYQITIGGNQGKNGGLTPGGQTPAAVGKVIGPA
                     FAQHEVPWVVERLIEVYLEKRESDAERFVDCAHRLGIEPFKQHVYGHPDSPRKDRAPV
                     PA"
     gene            complement(2817..3587)
                     /locus_tag="VFB53_03460"
     CDS             complement(2817..3587)
                     /locus_tag="VFB53_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN85822.1"
                     /translation="MEPSFILLGLAVGVLVGATGVGAGSLVTPALALAGVPPAVAVGT
                     DLAYAAVAKSAGALAHRAQRTLDLRVAGLLALGSVPAAALTVLALAATGAHHESGFVT
                     GALALALVLTALALLGGRARVAALAARYEPRIAPLRSPLTVAAGALLGVLVTVSSVGA
                     GALAAMFLVVLHPGMPAVRIAGTDIAHAVPLTLVAGLGHFWLGTVNLALAATLVVGAV
                     PGIVAGSLLAGRLPDALVRRLLALVLLLAGARLAAASF"
     gene            3661..4605
                     /gene="cysB"
                     /locus_tag="VFB53_03465"
     CDS             3661..4605
                     /gene="cysB"
                     /locus_tag="VFB53_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HZN85823.1"
                     /translation="MKIQQLRYLSEVARRDLSVSAAAEALFTSQPGISKQIKALEDEL
                     GIEIFARHGKRLTALTEPGRAVLAIAERILAEAANLKRAGEEFANEKLGSLTIATTHT
                     QARYALPKAVAAFKRRYPDVQLVLHQGTPTQICEMVIRGEAEFAVATEQISEFQELVS
                     LPCFQWNRCVVVPSGHPLARAKPLTLEAVAKYPIVTYDFAFANRSLVEKAFEQRGLKP
                     KVVLTALDADVIKTYVELGLGVGIMAAMAFHPKRDAGLKALDASHLFESSTTRLGIKR
                     GAYLRRYAYEFIELFVPHLPRSIVEPMVMGAEGSRYEL"
     gene            complement(4600..6087)
                     /gene="ubiD"
                     /locus_tag="VFB53_03470"
     CDS             complement(4600..6087)
                     /gene="ubiD"
                     /locus_tag="VFB53_03470"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HZN85824.1"
                     /translation="MHYADLREFLGKLEALGGLKRVVVPVSPRLEMTEICDRVLRAGG
                     PALLFERPAGHAMPVLGNLFGTVERIALAMGIDPGTDPAPALRDLGRLLAALREPDPP
                     QGLREVVQKLPQLAKLAGKVFDMAPRVLSSAPCQETVWEGKDVDLARLPVQTCWPGDA
                     GPLITWGLTVTRGPLKSRQNLGIYRQQLIGPDRVILRWLAHRGGALDYRDHQIARPGE
                     PFPVAVALGADPATTLAAVTPVPDTLGEYQFAGLLRGARTELAKCLGSELQVPARAEI
                     VLEGVIHPGDTALEGPHGDHTGYYNEAEQFPVLTVERVTLRRDPIYHSTYTGKPPDEP
                     AMLGVALNEIFVPLLTKQFPEIADFYLPPEGCSYRLAVASLKKQYPGHAKRVMFGIWS
                     YLRQFMYTKFIVVVDEDIDIRDWKEVVWAISTRVDPARDSLIAENTPIDYLDFASPVA
                     GLGSKMGIDATSKWPGETARKWGTPIRMDDSVKKKVDAMWKDLGL"
     gene            complement(6089..6616)
                     /locus_tag="VFB53_03475"
     CDS             complement(6089..6616)
                     /locus_tag="VFB53_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85825.1"
                     /translation="MPPESPIAAFDFASGPLRGTHLALYATRLLHHGQGYMESIPLPA
                     IAAVSVSYVRHEQRIGGGIALALLGVALFAAGGPLGAFAAGAAAEVTGAQSVAEVLRG
                     VLRALEAFAAVLPVAGVAGVAGGAALVVFGWIGTTTLSLLLPAAERAYEVRGQNRLLV
                     DFADLLAERVAQRPH"
     gene            complement(6628..7500)
                     /gene="ubiA"
                     /locus_tag="VFB53_03480"
     CDS             complement(6628..7500)
                     /gene="ubiA"
                     /locus_tag="VFB53_03480"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZN85826.1"
                     /translation="MDLSFVGKRLDAYERLLRLDKPIGTLLLLWPTLWALWLAARGTP
                     RVEVLAVFCVGTVLMRSAGCAVNDFFDAAFDRQVERTRDRPLAAGEIRPAEAMALAGL
                     LAAAAFSLVFLLNLLALYLSFVALAIAVTYPLAKRFFALPQLYLGIAFGFGIPMAYAA
                     VRNWLPPECWVLLAANVCYAFAYDTEYAMVDREDDAKLGLRTSALTLGRYDVAGVMLA
                     YGGMLALLAAVGVMTKLAWPYYAGLALAAGLMGYHFLLIRGRSREGCFRAFRHNNWVG
                     AAVFGGIVAATALR"
     gene            complement(7510..9513)
                     /gene="recG"
                     /locus_tag="VFB53_03485"
     CDS             complement(7510..9513)
                     /gene="recG"
                     /locus_tag="VFB53_03485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZN85827.1"
                     /translation="MTRAIPPATAKKLERLGIRSRAELLYHLPLRYEDETALTPLGRA
                     LPGCAVLVEAPVRRVEVAYRGTRQLLVHCDGIALRFMNFYPNQKKSFEDAIANRKLVR
                     AYGEVRPARGGAEMIHPRTRLVAPGEPLPATLTPVYPTTAGLGQATLAKLVQAALAEE
                     PIEDNLSAQLRARYALAPLEASVRLLHAPAPGADLAGAWRRVKFDELLAQQLQLRLAR
                     RKRRERSAPALPSNGPLLRRFLGKLPFRLTRAQQRVMAELLQDLAQPHPMQRLLQGDV
                     GSGKTIVAAIGCLAAVDAGAQAAVMSPTEILAEQHHRKFSDWLAPLGVRIAWLHGGLP
                     APQRKAARRAIAAGEAQIAIGTQALFQEGVEFDRLALAIVDEQHRFGVRQRLELRKKS
                     ERAMAHQLMMSATPIPRTLAMTRDADLDVSVIDELPPGRKPVTTHTYSAARRDAVLAR
                     IRAACAEGQQAYWVCPVIDESKEELQAALATFEDLRRELAALRVGLLHGRMPPAEKAA
                     AMGAFAAGATQLLVCTTVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRIGRGALAS
                     ECVLLFQEPLSEHARRRLKVIRETADGFAIAEEDLRLRGPGEFLGERQSGEVPLRFAD
                     LAADEALILQAREAAEALLDAGGDFARRHIDRWLGARAEYQKV"
     gene            complement(9510..9887)
                     /locus_tag="VFB53_03490"
     CDS             complement(9510..9887)
                     /locus_tag="VFB53_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN85828.1"
                     /translation="MKSAIHTPDAPAAIGPYSQAVRAGDTLYLSGQIGLDPKTMQLAE
                     GVEAQIERVFANLAAVARAAGLGLERTVRMTVYLTDLAHFARVNDSMARHFSEPYPAR
                     AAVGVASLPRGALVEIDAILHAG"
     gene            9933..10265
                     /locus_tag="VFB53_03495"
     CDS             9933..10265
                     /locus_tag="VFB53_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN85829.1"
                     /translation="MPSPLIASLEKLVGTPRDGALLRYSLGTEYLKAGDAGRGIASLR
                     EAVARDPQYSAAWKALGRALAEAGRDEEALAAFASGIEAARARGDKQAGKEMAVFARR
                     LEKKPGAR"
     gene            complement(10267..>11287)
                     /locus_tag="VFB53_03500"
     CDS             complement(10267..>11287)
                     /locus_tag="VFB53_03500"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZN85830.1"
                     /translation="ADMARPDDAALVGQKMLESLAVPFDLGGNEAYVTGSIGIAVYPG
                     DGGDAETLLKNADMAMYRAKESARNSYCFFTAEMNQRSVAKVQLNADLRHALERDEFA
                     LHYQPKLDLATGELRGLEALLRWNHPSRGLVSPAEFIPALEDSGLIVPVGEWVVEQAC
                     RQLRLWTAAGLAPLPVAVNLSPKQFRRRDLDALIRNALVRSGISAEFLELEITESCLM
                     DNPEDAVRAMHNLRAAGLRISIDDFGTGYSSLSYLTRLPLSALKIDRSFVRDSGTSAE
                     SASIVRAIIDMAQNLHLTVIAEGVETEEQAAFLRREGCHQAQGFLYSRPLPAAEIAAR
                     LAPAG"
BASE COUNT         1620 a         4029 c         4091 g         1547 t
ORIGIN      
        1 cttccatggt ctccggtttt ttggcggcat ggggttcgcc gccaaaaaac cggagatcgt
       61 ttttaaaggc aactgcactg gcaccggccg cggcaggccc cgccacatgc aacccgcact
      121 ctttcttccc ttcctcctcc caccaccagc gtcccgcgcg ctcgtcctcg cccggcgcca
      181 cggcgcgcgt gcagggctcg cagccgatgg acgggtagcc gcgctcgtgc agcgggttga
      241 ccggcacctt gtgggcgcgg acgtactccc acacgtcgtt gccgctccac tcggcgagcg
      301 ggttgaactt ggccaggtgc agcgcggcgt cgaactcggc gaggcgcagg cccgcgcgcc
      361 cggcggactg gtcgcggcgc aggccggtga tccaggcgcg cttgccggca agcgcgcgcc
      421 tcagcggctc caccttgcgg atctcgcagc aacgcttcct caactcgacg ctgccgtaga
      481 aggcgttgcg cccgtgcgtg gtcacgtact ccaccaccgc ctgcgcttcg ggctgcatca
      541 cccgcagcgc gatgccgtag tgcgactgca cgcgcccgag catgtcgcgc gtctcggcgt
      601 gcagccgccc ggtgtccagg gtgaagatct cgatcggcag gccctcgcgc gcgatgagat
      661 cggtgagcac catgtcctcg gcgccgaggc tggacgagag cgccgccggc gcatacgccg
      721 tggcgatgcg ctccaggagc aggcgggcgt cggcggcgcg gctcatcggc ggcgaaacag
      781 gggctgcggc cgctcgaccg aggcctggta ggcctcggag aagtcccgca gggcggcgag
      841 cgcggcctcg gcgtcctcgc cctcgcgcag gaggaaggcg tcgaagccgc agcgcgagag
      901 gtagaagatc tggtcgcgct gcacgtcgcc caccgcgcgc agctcgccct tgtagccgtg
      961 gcgctcgcgc agcaggcgcg cgatggagta gccgcgcccg tcgttgaacc tggggaagtt
     1021 cacctcgacg cgcgcggccg cgcccaggcg atcggccacc ccggccgggt cgtcggccgg
     1081 ctccaggcgc agcacctcgc ccgcctcagg cggggacggg tgcgcgatct ttccgcggcg
     1141 aatcagggtg gccatagacg tgctgcttga acggttcgat gccgaggcgg tgcgcgcaat
     1201 ccacgaagcg ctcggcgtcg ctttcgcgtt tctccaggta cacctcgatc aggcgctcca
     1261 ccacccaggg cacctcgtgc tgggcgaacg ccgggccgat caccttgccg acggcggctg
     1321 gggtctgacc ccctggggtc agacccccgt ttttgccctg attcccgccg atggtgatct
     1381 ggtagaactc ctcgccctgc ttgtccacgc ccagcacgcc gatgtggccg acgtggtggt
     1441 ggccgcagga attgatgcag ccggagatgt tgagatcgag ctcgccgatg tcgtgcaggt
     1501 aatccaggtc gtcgaacagg cgctggatcg cctcggcgac ggggatcgac ttggcgttgg
     1561 cgagcgagca gaaatccccg cccgggcagg cgatgatgtt ggtcagcagg ccgatgttcg
     1621 gcgtggcgag gcccagctcg cgcgcctcgc gccagagcac ctcgagctgg tcctggcgca
     1681 cgtcggcgag caccaggttc tgctcgtgcg tgacgcgcag ctcgccgaag ctgtagcgct
     1741 cggcgaggtc ggcgacggcg tccatctgcg cgtcggtggc gtccccgggc ggcacgccgg
     1801 tgcgcttgag cgacagcgtc accgcggcgt agcccggctg cttgtgcggg aacacgttgc
     1861 gcttcagcca ggcgccgaag ggcccctcct gcgcgaggcg caggtcttcc agcggctggt
     1921 agcgcgggcg cgtgaagcgg cccgccacgc ggcgcagctc ggcctcggtg agcgtcgagg
     1981 ggccgtcctt cagctgcgcc cactcggcct cgacctcgtc gcggaacttc tccggcccgc
     2041 gctccttcac caggatcttg atgcgcgcct tgtgcatgtt gtcgcgccgc ccgtagcggt
     2101 tgtacacgcg caggatcgcc tcgacgtagg acagcaggtg ctgccagggg aggaactcgc
     2161 ggatcgcgtg gccgatgagc ggcgtgcggc ccatgccgcc gccgacgatg acgcggaacc
     2221 ccaccgcgcc cgccgcgtcg cgcaccgcgt gcagcccgat gtcgtgcagg aaggtggcgg
     2281 cgcggtcggc ctcggcgccg ttgacggcga tcttgaactt gcgcggcagg taggcgaact
     2341 cgggatggaa ggtcgaccac tggcgcagca cctcgcacca cacgaacgag tcgacgatct
     2401 cgtcgcgcgc cacgccgccg aaatggtccg ccgtgatgtt gcgcacgcag ttgcccgagg
     2461 tctggatggc gtgcatctgc acgctcgcca ggtcggcgag gatgtcgggc acctgctcga
     2521 gcttgggcca gttgaactgg atgttctggc gcgtggagaa gtggccgtag ccgcggtcgt
     2581 acttcctcgc gatgtgcgcg agcatgcgca gctgccgcga ggacaggatg ccgtagggga
     2641 cggccacgcg cagcatcggc gcgtagcgct gcacgtacag gccgttctgc agccgcaagg
     2701 ggcggaaatc gtcctccttc agcttgccct cgaggaagcg ccgcgtctgg tcgcggaact
     2761 gcgcgacgcg ctcgtcgacg attttctggt cgatctcgtc gtagaggtac atggcgctag
     2821 aagctcgccg cggcgaggcg cgcgccggcg agcagaagga cgagggcgag caggcgccgc
     2881 accagcgcgt cgggcaggcg ccccgcgagc aggctgccgg cgacgatgcc gggcaccgcg
     2941 ccgacgacga gggtcgccgc gagcgcgagg ttcaccgtgc cgagccagaa gtggccgagg
     3001 cccgcgacca gcgtgagcgg caccgcgtgc gcgatgtcgg tgccggcgat gcgcaccgcc
     3061 ggcatgcccg gatgcagcac caccaggaac atggcggcga gcgcccccgc gccgaccgac
     3121 gagacggtga ccagcacgcc gaggagcgcg ccggccgcca cggtgagcgg cgagcgcagc
     3181 ggcgcgatgc gcggctcgta gcgcgcggcg agcgcggcga cgcgcgcgcg tccgccgagc
     3241 agcgcgagcg cggtgaggac cagcgccagg gcgagcgcgc cggtgacgaa gccgctttcg
     3301 tgatgcgccc cggtcgccgc gagcgcgagg acggtgagcg ccgccgccgg gacgctgccc
     3361 agcgccagca ggcccgcgac gcgcaggtcc agggtgcgct gcgcgcggtg cgcgagcgca
     3421 ccggccgact tggccaccgc ggcataggcg aggtcggtgc ccaccgccac ggccggcggc
     3481 acgcccgcca gcgcgagcgc cggggtgacg agcgacccgg cgccgacgcc ggtcgcgccc
     3541 accagcacgc ccaccgcgag gccgagcagg atgaaagacg gctccatggc ccggaattct
     3601 gggcccccgc cttatgcttt caaagactga tgtgttagat tcttatgctt tattgatctt
     3661 atgaaaatcc agcagctccg ctacctgtcc gaagtcgcgc gccgcgacct gagcgtctcc
     3721 gccgcggccg aggccctgtt cacctcccag ccaggaatct ccaagcagat caaggccctg
     3781 gaggatgagc tggggatcga gatcttcgcc cgccacggca agcgcctcac cgcgctcacc
     3841 gagcccgggc gcgcggtcct ggccatcgcc gagcgcatcc tcgcggaagc cgccaacctg
     3901 aagcgcgcgg gcgaggagtt cgccaacgag aagctgggct cgctcaccat cgccaccacc
     3961 cacacccagg cgcgctacgc gctgcccaag gcggtggccg cattcaagcg ccgctacccg
     4021 gacgtgcagc tggtgctgca ccagggcacc ccgacgcaga tctgcgagat ggtgatccgc
     4081 ggcgaggcgg agttcgcggt ggccaccgag cagatctcgg agttccagga gctggtctcg
     4141 ctgccctgct tccagtggaa ccgctgcgtg gtggtgccct cgggccaccc gctcgcgagg
     4201 gcaaagccgc tcaccctgga ggcggtggcg aagtacccga tcgtcaccta cgacttcgcc
     4261 ttcgccaacc gctcgctggt ggaaaaagcg ttcgagcagc gcggactgaa gccaaaggta
     4321 gtgctcactg cgctcgacgc ggacgtgatc aagacctacg tcgagctggg cctgggggta
     4381 gggatcatgg cggcgatggc cttccacccg aagcgcgacg ccggcctgaa ggcgctcgac
     4441 gcgagccacc tcttcgaatc gagcaccacg cgcctcggca tcaagcgcgg ggcgtacctg
     4501 cggcgctatg cctacgagtt catcgagctc ttcgtccccc acctgccgcg ctcgatcgtc
     4561 gagccgatgg tgatgggcgc cgaaggctcg cgctacgagc tatagcccca ggtccttcca
     4621 catcgcgtcg accttctttt tcacgctgtc gtccatgcgg atcggggtgc cccatttgcg
     4681 cgcggtctcg ccgggccact tgctggtggc gtcgatgccc atcttggagc cgaggcccgc
     4741 caccgggctg gcgaagtcga ggtagtcgat cggcgtgttc tccgcgatga gcgagtcgcg
     4801 cgcgggatcg acgcgcgtcg agatcgccca caccacttcc ttccagtcgc ggatgtcgat
     4861 gtcctcgtcc accaccacga tgaacttggt gtacatgaac tgccgcaggt agctccagat
     4921 gccgaacatc acgcgcttgg cgtgccccgg atactgtttc ttgaggctcg ccaccgcgag
     4981 ccggtacgag cagccctcgg gcggcaggta gaagtccgcg atctccggga actgcttggt
     5041 gagcagcggc acgaagatct cgttcagcgc cacgcccagc atcgccggct cgtcgggcgg
     5101 cttgccggtg taggtggagt ggtagatggg gtcgcggcgc agggtcacgc gctccaccgt
     5161 cagcaccggg aactgctcgg cctcgttgta gtagccggtg tggtcgccgt gcggcccctc
     5221 gagcgccgtg tccccggggt ggatcacgcc ttcgagcacg atctcggcgc gcgccggcac
     5281 ctgcagctcg ctccccaggc acttggcgag ctcggtgcgc gcgccgcgca gcagcccggc
     5341 gaactggtat tcgcccagcg tatcgggcac cggggtcacc gcggcgagcg tggtcgccgg
     5401 gtcggcgccg agcgccaccg cgaccgggaa cggctcaccc ggcctcgcga tctggtggtc
     5461 gcggtaatcg agcgcgccgc cgcggtgcgc gagccagcgc aggatcacgc ggtccggccc
     5521 gatcagctgc tggcggtaga tgccgaggtt ctggcgcgac ttgagcggcc cgcgcgtcac
     5581 ggtgagcccc caggtgatga gcggccccgc gtcgcccggc cagcaggtct gcaccggcaa
     5641 tcgcgcgagg tccacgtcct tgccctccca caccgtctcc tggcagggcg ccgaggacag
     5701 caccctgggc gccatgtcga acaccttgcc ggcgagcttc gcgagctgcg gcagcttctg
     5761 caccacctcg cgcaggccct gcggcgggtc gggctcgcgc agcgcggcga gcagccggcc
     5821 gaggtcgcgc agcgcgggcg cgggatccgt cccgggatcg atgcccatcg ccagcgcgat
     5881 gcgctccacc gtgccgaaga ggttgcccag cacgggcatg gcgtggcccg cggggcgctc
     5941 gaacagcagc gccgggccgc ccgcgcgcag cacgcggtcg cagatctcgg tcatctccag
     6001 gcgcggcgac accggcacga cgacgcgctt caggcccccc agcgcctcga gcttgccgag
     6061 gaattcgcgc aggtcggcgt agtgcacgtc agtgcgggcg ctgcgccacg cgctcggcga
     6121 gcaggtcggc gaaatccacc agcaggcggt tctgcccgcg cacctcgtag gcgcgctccg
     6181 ccgcgggcag caggagcgac agcgtggtgg tgccgatcca gccgaacacg acgagcgccg
     6241 cgccgccggc gacgccggcc acccccgcca ccggcagcac cgccgcgaag gcctccagcg
     6301 cgcgcagcac cccgcgcagc acctcggcga ccgactgcgc gcccgtcacc tcggccgccg
     6361 cgcccgcggc gaacgcgccc agcggcccgc cggcggcgaa cagcgccacg ccgagcagcg
     6421 cgagcgcgat gccgccgccg atgcgctgct cgtggcgcac gtagctcacg ctgaccgcgg
     6481 cgatggcggg caggggaatg ctctccatgt agccctggcc gtggtgcagc agccgcgtgg
     6541 cgtacagggc gaggtgcgtg ccgcgcagcg gcccggaggc gaagtcgaag gcggcgatgg
     6601 gggattcggg cggcatggcg gctgcggtta cctgagggcg gtggcggcga cgatgccgcc
     6661 gaacaccgcg gcgcccaccc agttgttgtg gcggaaggcc cggaagcagc cctcgcgcga
     6721 gcgcccgcgg atcaggagga aatgatagcc catgaggccg gcggcgaggg cgagcccggc
     6781 gtaatagggc caggcgagct tcgtcatcac gcccaccgcg gcgagcagcg ccagcatgcc
     6841 gccataggcg agcatcaccc cggcgacgtc gtagcgcccc agcgtcagcg ccgaggtgcg
     6901 caggccgagc ttcgcgtcgt cctcgcggtc caccatcgcg tactcggtgt cgtaggcgaa
     6961 ggcgtagcac acgttcgccg ccagcagcac ccagcattcc ggcggcagcc agttgcgcac
     7021 cgcggcgtag gccatcggga tgccgaagcc gaaggcgatg ccgaggtaga gctgcggcag
     7081 cgcgaaaaag cgcttggcga gcgggtaggt gacggcgatg gcgagcgcga cgaacgacag
     7141 gtacagcgcg agcaggttga gcaggaacac caggctgaag gccgcggcgg cgaggaggcc
     7201 cgcgagcgcc atcgcctcgg ccggccggat ctcccccgcg gcgagcggcc ggtcgcgcgt
     7261 gcgctcgacc tggcggtcga aggcggcgtc gaagaagtcg ttcaccgcgc agcctgccga
     7321 gcgcatcagc accgtgccga cgcagaacac ggcgagcacc tccacgcgcg gcgtgccgcg
     7381 cgcggcgagc cacagcgccc acagcgtcgg ccagagcaac agcagcgtgc cgatcggctt
     7441 gtccagccgc agcagccgct cgtaggcgtc gagccgcttg ccgacgaagc tcaagtccat
     7501 cgcgcgagtc tagaccttct ggtactcggc gcgcgcgccc agccagcgat cgatgtggcg
     7561 gcgcgcaaaa tccccgccgg cgtcgagcag cgcctccgcc gcctcgcgcg cctgcaggat
     7621 cagcgcctcg tccgcggcga gatcggcgaa gcgcagcggc acctcgccgc tctggcgctc
     7681 gccgaggaat tcgcccggcc cgcgcaggcg caggtcttcc tccgcgatgg cgaagccgtc
     7741 ggccgtctcg cggatcacct tgaggcgccg gcgcgcatgc tccgagagcg gctcctggaa
     7801 cagcagcacg cactcgctgg cgagcgcgcc ccgcccgatg cggccgcgca gctggtgcag
     7861 ctgcgagagg ccgaaccgct cggcgtgctc gatcaccatc agcgaggcgt tgggcacgtc
     7921 cacgccgacc tcgatcaccg tggtgcagac gaggagctgc gtggcgccgg cggcgaacgc
     7981 gcccatcgcc gcggccttct ccgccggcgg catgcgcccg tgcagcaggc cgacgcgcag
     8041 cgcggcgagc tcgcggcgca gatcctcgaa ggtcgccagc gcggcctgca gttcctcctt
     8101 cgattcgtcg atcaccgggc acacccagta ggcctgctgg ccttccgcgc aggccgcgcg
     8161 gatgcgcgcc agcaccgcgt cgcgccgcgc cgccgaatag gtgtgcgtgg tcaccggctt
     8221 cctgcccggg ggcagctcgt cgatcaccga gacgtcgagg tcggcgtcgc gcgtcatcgc
     8281 cagcgtgcgc gggatcgggg tggcgctcat catcagctgg tgcgccatgg cgcgctcgct
     8341 ctttttcctc agctccaggc gctggcgcac gccgaagcgg tgctgctcgt cgacgatggc
     8401 gagcgcgagg cggtcgaact ccacgccctc ctggaacagc gcctgggtgc cgatggcgat
     8461 ctgcgcctct cccgcggcga tcgcccggcg cgcggccttc ctctgcggcg ccggcagccc
     8521 gccgtgcagc caggcgatgc gcacgcccag gggcgcgagc cagtcggaga acttgcggtg
     8581 atgctgctcg gcgaggattt ccgtgggcga catcaccgcg gcctgcgcgc ccgcgtccac
     8641 cgccgcgagg cagccgatcg cggcgacgat ggtcttgccg ctgcccacgt cgccctgcag
     8701 caggcgctgc atggggtgcg gctgcgccag gtcctgcagc agctcggcca tcacgcgctg
     8761 ctgcgcgcgc gtgaggcgga acggcagctt gccgaggaag cggcgcagca gcggcccgtt
     8821 cgaaggcagc gccggcgcgc tgcgctcgcg ccgcttgcgg cgcgccaggc gcagctgcag
     8881 ctgctgcgcc agcagctcgt cgaacttcac ccgccgccag gccccggcga ggtccgcgcc
     8941 gggcgcgggc gcgtgcagca ggcgcacgct cgcctcgagc ggcgcgagcg cgtagcgcgc
     9001 gcgcagctgc gccgaaaggt tgtcctcgat cggctcctcc gcgagcgccg cctgcacgag
     9061 cttcgcgagc gtcgcctggc cgaggccggc ggtggtcggg tacacggggg tgagcgtggc
     9121 gggcagcggc tcgcccggcg ccaccaggcg ggtgcgcggg tggatcatct ccgcgccgcc
     9181 gcgcgccggg cggacttcgc cgtaggcgcg caccagcttc cggttcgcga tcgcgtcctc
     9241 gaagctcttc ttctggttcg gatagaagtt catgaagcgc agcgcgatgc cgtcgcaatg
     9301 caccagcagc tggcgcgtgc cgcggtatgc cacctcgacg cgccgcaccg gcgcctcgac
     9361 cagcaccgcg caccccggca gcgcgcgccc cagcggcgtg agcgcggtct cgtcctcgta
     9421 gcgcaggggc aggtggtaca gaagctccgc gcgcgagcgg atgccgagcc gctcgagctt
     9481 cttcgccgtc gcgggaggga tcgcgcgcgt caaccggcgt gcaggatggc gtcgatctcg
     9541 accagcgcgc cgcgcggcag gctggccacg ccgaccgcgg cgcgcgccgg gtagggctcg
     9601 ctgaagtgcc gcgccatgct gtcgttgacc ctggcgaagt gcgccaggtc ggtcaggtac
     9661 accgtcatgc gcacggtccg ctccaggccg aggccggcgg cgcgcgccac cgccgcgagg
     9721 ttggcgaaca cgcgctcgat ctgcgcctcg acgccctcgg cgagctgcat ggtcttcggg
     9781 tcgaggccga tctggcccga caggtacagg gtgtcgccgg cgcgcaccgc ctgcgagtaa
     9841 gggccgatcg ccgccggcgc gtcgggggtg tggatcgcgg atttcatggg gagcccgggt
     9901 ctagaatgga cgcggggaat gttagcccat cgatgccctc gccgctgatc gccagcctgg
     9961 agaagctcgt cggcacgccg cgcgacggcg cgctgctgcg ctattcgctc ggcaccgagt
    10021 acctgaaggc gggcgacgcc gggcgcggca tcgccagcct gcgcgaggcg gtggcgcgcg
    10081 acccgcagta ttccgcggcc tggaaggcgc tggggcgcgc gctggccgag gcggggcgcg
    10141 acgaggaggc gctcgccgcc ttcgccagcg gcatcgaggc cgcgcgcgcg aggggagaca
    10201 agcaggccgg caaggagatg gcggtgttcg cgcgccggct ggaaaaaaag cccggcgcgc
    10261 gctgactcag ccggcgggcg cgagccgcgc ggcgatctcc gccgccggca gcggccggct
    10321 gtagaggaag ccctgcgcct ggtggcagcc ctcgcggcgc aggaaggcgg cctgttcctc
    10381 ggtctccacg ccctcggcga tcaccgtgag gtgcaggttc tgcgccatgt cgatgatggc
    10441 gcgcacgatc gaggccgact cggcgctggt gcccgagtcg cgcacgaagc tgcggtcgat
    10501 cttcagcgcc gacagcggca gccgcgtgag gtaggacagg ctcgagtagc cggtgccgaa
    10561 gtcgtcgatc gagatccgca ggcccgcggc gcgcaggttg tgcatcgcgc gcaccgcgtc
    10621 ctcggggttg tccatcaggc agctctcggt gatctccagc tcgaggaact ccgccgagat
    10681 gcccgagcgc accagcgcgt tgcggatcag cgcgtcgagg tcgcggcggc ggaactgctt
    10741 gggcgacagg ttcaccgcca ccggcagcgg cgcgaggccg gccgcggtcc acaggcgcag
    10801 ctggcggcag gcctgctcca ccacccactc gcccaccggc acgatgaggc ccgagtcctc
    10861 cagcgccggg atgaactccg ccggcgacac caggccgcgc gaggggtggt tccagcgcag
    10921 cagcgcctcc agcccgcgca gctcgcccgt ggcgaggtcc agcttgggct ggtagtgcag
    10981 cgcgaactcg tcgcgctcca gggcgtggcg caggtcggcg ttcagctgca ccttggccac
    11041 cgagcgctgg ttcatctcgg cggtgaagaa gcagtagctg ttgcgcgccg actccttggc
    11101 gcggtacatc gccatgtcgg cgttcttcag cagcgtctcg gcgtcgccgc cgtcgcccgg
    11161 gtacaccgcg atgccgatcg agccggtgac gtaggcttcg ttgccgccga ggtcgaaggg
    11221 cacggcgagc gactcgagca tcttctgccc gaccagcgcg gcgtcgtccg ggcgcgccat
    11281 gtcggcg
//
LOCUS       DASRIE010000073         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_602486, whole genome shotgun sequence.
ACCESSION   DASRIE010000073 DASRIE010000000
VERSION     DASRIE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602486"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFB53_03505"
     CDS             complement(<1..426)
                     /locus_tag="VFB53_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85831.1"
                     /translation="MSAPRVVIIGAGPAGLTAAHELAENGVRDIVVLEASGAIGGLSK
                     TVNYRGNRIDIGGHRFFSKSDWVMDWWLRMLPLEAGAEQGLDIGYQGKQRRVAPSSGA
                     AATQQRVMLVRNRLSRIYFNGRFFDYPLKVNFATARKLGL"
     gene            complement(423..854)
                     /locus_tag="VFB53_03510"
     CDS             complement(423..854)
                     /locus_tag="VFB53_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZN85832.1"
                     /translation="MRARAFSLAELTALAREGARYAIASAIALALDFALYAGLIRLAD
                     VHYLVAAPAGFMLGLVTIYLLSAHWAFAARRYDDARVEFAVFAAIGGAGLLLNQAVIY
                     AGVSGAELSPEAAKLVSAALVFTFNFSARKLLLFTRPGGGA"
     gene            complement(826..>2534)
                     /locus_tag="VFB53_03515"
     CDS             complement(826..>2534)
                     /locus_tag="VFB53_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85833.1"
                     /translation="DDVYFRSGSQDRYTLFTPILAWLGAHLGLGRAHLVLTVAPLALF
                     LAASYALVRALLPAGLRGFSMLLVAASAGTYGSHRLFRMAEPFVTPRPLAEAMTLASL
                     ALLFTGRRWWALALLGACALLHPLVAAAGLLVWWIHALLTGANRRILLALALAPLAAG
                     LAGVAPFDLLFATFDVEWFDKIYGSRQLFILQWIWVDWASLLFDLTVIVLAERVSDGP
                     LRGVFRAARWTAVVAVLATFAGADAFHNVLLTNLQPWRALWIVHWLALAGLTLVVART
                     WNESEGGRLIAALLLFAFVTRGTQTSAAATLLAAALFAARAQLVVTPGLIPGLIAALG
                     AGALASWVNVDFVLFRSPLFPVGSVEDPWMRVLTRTFPVLVLAAAVVWFGLRRSAGWS
                     AAAATAALAVSLAAWDQRSPFARYVDSTEPGQHPFSRIVAPGEQIWWHGSSLSDGRSV
                     VLPWIMMKRPSFQSAFQHVGQHFNREVTFELERRDQLTLPFQFQAGICEILNLIDPRE
                     HCQPDFEALQELCREAPELHYLALETDYPGKWITAWSPPAAIAGKVPRFYLYACKGIL
                     AR"
BASE COUNT          452 a          890 c          898 g          294 t
ORIGIN      
        1 caggccgagc ttgcgcgcgg tcgcgaaatt caccttaagg ggatagtcga agaagcggcc
       61 gttgaagtag atgcgcgaaa gccggttgcg caccagcatg acccgctgtt gcgtggccgc
      121 ggcgccggaa gacggcgcca cgcgccgctg cttgccctgg tagccgatgt ccaacccctg
      181 ctccgcgccc gcttcgagcg gcagcatgcg cagccaccag tccatcaccc agtcggactt
      241 ggagaaaaag cggtgaccgc cgatgtcgat gcggttaccg cggtaattca ccgtcttcga
      301 cagtccgccg atcgcgccgc tcgcctccag caccacgatg tcgcgcacgc cgttctcggc
      361 gagctcgtgc gccgcggtca ggccggcggg gccggcgccg atgatcacca cgcgcggcgc
      421 gctcacgccc cgcccccggg gcgcgtgaag aggagcagct tccgcgccga gaaattgaag
      481 gtgaagacga gcgccgccga gaccagcttc gccgcctcgg gcgaaagctc ggcgccgctc
      541 acgcccgcgt agatcaccgc ctggttgagt agcagcccgg cgccgccgat cgcggcgaac
      601 accgcgaatt cgacacgcgc gtcgtcgtag cgccgtgccg cgaacgccca gtgcgcgctc
      661 agcaggtaga tcgtgaccag gccgagcatg aagccggccg gcgccgccac caggtaatgc
      721 acgtcggcga gccggatcag gcccgcgtaa agcgcgaagt cgagcgccag cgcgatcgcc
      781 gaggcgatcg cgtagcgcgc gccctcgcgc gcgagcgcgg tcagctcagc gagcgagaat
      841 gcccttgcac gcatagaggt agaagcgcgg caccttcccg gcgatcgccg ccggcgggct
      901 ccacgccgta atccattttc ccgggtagtc ggtttccagc gctagataat ggagttccgg
      961 cgcctcccgg catagctcct gcagggcctc gaagtccggc tggcagtgtt cccgcggatc
     1021 gatcaggttc aggatctcgc agatccccgc ctggaactgg aacggcaggg tcagctggtc
     1081 gcggcgctcg agctcgaacg tgacctcccg gttgaaatgc tggccgacat gctggaacgc
     1141 ggactggaag ctcgggcgct tcatcatgat ccagggcagc accacgcttc tgccgtcgga
     1201 caggctcgac ccgtgccacc agatctgctc ccccggcgcc acgatgcgcg agaagggatg
     1261 ctggccgggc tcggtggaat ccacgtacct cgcgaatggc gagcgctggt cccaggcggc
     1321 gagcgacacc gcgagggctg cggtcgcggc cgcggcgctc cagcccgcgc tgcggcgcag
     1381 gccgaaccac accaccgcgg cggcgagtac cagcaccggg aaggtgcgcg tcagcacccg
     1441 catccacgga tcctccaccg agcccactgg aaacagcggg ctgcggaaca ggacgaagtc
     1501 gacattgacc cagctcgcca gcgccccggc gccgagcgcg gcgatcaggc ccgggatcag
     1561 ccccggggtg accacgagct gcgcgcgggc ggcgaacaat gccgccgcga gcagcgtcgc
     1621 cgccgccgaa gtctgcgtgc cgcgggtgac gaaggcaaac agcaggagcg ccgcgatcag
     1681 gcggcccccc tcgctctcgt tccaggtgcg tgcgaccacg agcgtgagcc ccgccagcgc
     1741 gagccagtgc acgatccaca gcgcgcgcca gggctgcagg ttggtgagga gcacgttgtg
     1801 gaaggcgtcg gcgccggcaa acgtggcgag caccgcgacc accgcggtcc agcgcgctgc
     1861 gcggaagacg ccgcgcagcg gaccgtcgct gacgcgctcg gcaaggacga tcaccgtgag
     1921 atcgaacagc aagctcgccc agtccaccca gatccactgc aggatgaaga gttgccgact
     1981 accgtagatc ttgtcgaacc actcgacgtc gaacgtcgcg aacagcaggt cgaacggcgc
     2041 cacgccggcg aggccggcgg cgaggggcgc cagtgccaag gcgaggagga tgcgccggtt
     2101 cgcgccggtg agcagcgcgt ggatccacca gacgagcagg ccggccgccg cgacgagcgg
     2161 atgcagcagc gcgcaggcgc cgagcagcgc gagcgcccac cagcgccggc cggtgaacag
     2221 cagggccagg gacgccagcg tcatcgcctc ggcgagcggc cgcggcgtca cgaacggctc
     2281 ggccatgcgg aacaagcggt gcgagccgta ggtgccggcg ctcgccgcga ccagcagcat
     2341 cgagaagccg cgcagcccgg cgggcagcag cgcgcgcacc agggcgtacg aggcggcaag
     2401 gaacagggcg agcggcgcca ccgtcagcac gaggtgggcg cggcccaggc cgaggtgcgc
     2461 gccgagccac gccaggatcg gcgtgaacag cgtgtagcgg tcctgcgagc ccgagcggaa
     2521 gtagacgtcg tcgc
//
LOCUS       DASRIE010000074         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_74319, whole genome shotgun sequence.
ACCESSION   DASRIE010000074 DASRIE010000000
VERSION     DASRIE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74319"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFB53_03520"
     CDS             complement(<1..488)
                     /locus_tag="VFB53_03520"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HZN85834.1"
                     /translation="MKILFVHQNFPGQYKHLAAHFARNPKHQVVSIGERKDERQVSLK
                     GMRHLWYDKPKGANPTTHLYLQGIEGAVRRGQAVVRTCLELRKGGFVPDVICAHPGWG
                     EALYLKEVFPEAKLLNFYEFYYGARGSDLGFDPEFPTSFDDRFKVPTRNATHLLSLAS
                     GD"
     gene            complement(485..1327)
                     /locus_tag="VFB53_03525"
     CDS             complement(485..1327)
                     /locus_tag="VFB53_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85835.1"
                     /translation="MRILFTIPHYYDPAGGGKYGSLKADSAPRRNALAGAIFSLHANL
                     GRQQAFLGAPTVACNAAATADLEVVVCTTGGRHLLDEMRLPPDSFRHHATQAQPMYLG
                     FECHEVLRQGLGRFDWFCYLEDDIALLDPLFFHKLEWFNRLAGDEAVLQPNRNEIAIG
                     QRWHKLFVDGELSRPEWSTRWQDVNDRPPIEAEAFGVRVRFVRAANPHSGCFFLNARQ
                     MERWTQQPGFLAREPSFVGPLESAASFGILRHFRTYKPAPENAGFLEVRHLDNRYLGA
                     KKKP"
     gene            complement(1334..2320)
                     /locus_tag="VFB53_03530"
     CDS             complement(1334..2320)
                     /locus_tag="VFB53_03530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN85836.1"
                     /translation="MNKIGIAAWLVLFAGAAASQQLQLPLQCTPGADCWISNHVDLDP
                     GPQARDYACGTFTYNDHNGTDIALRDAAAMDQGVKVLAAAGGVVRATRDGVEDVSVRA
                     RGRDAIAGIECGNAVRIEHGGGLQTQYCHLRRGSVAVKRGERVRAGQPLGLVGLSGLT
                     EYPHLHFTVRRGGAVLDPFRGEGAGPACGPGERPLWDAATLDALGYAPPAIYNYGVAA
                     RIPPESEVRSGAYRARELARDAPALMVWAEAFGTTGGETLRVRVEAPDGTTRFEQKIV
                     LDRPLARVYRAVGRKRGAEPWASGTWWVTIAAEKTAPAGRAPPPIRFSFEVR"
     gene            2343..>2741
                     /locus_tag="VFB53_03535"
     CDS             2343..>2741
                     /locus_tag="VFB53_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN85837.1"
                     /translation="MRPPQRSDNVTQALQTAREHLRAGRHAEAEACLAPLVKVNPKHA
                     AALQLLGVIALARRDFAAAADFLEKSLAADPRQVQAWNNYGAACQALGRLEQAIGAFR
                     RALELQPGHAGALNNLGVLHARRNELTEAEA"
BASE COUNT          383 a          965 c          995 g          398 t
ORIGIN      
        1 cagtcccccg aggcgaggct gaggaggtgc gtggcgttgc gcgtcggcac cttgaagcgg
       61 tcgtcgaagg aggtggggaa ttccgggtcg aagcccaggt ccgagccgcg cgcgccgtag
      121 tagaactcgt agaagttaag cagcttcgct tcggggaaca cttccttgag atagagcgcc
      181 tcgccccagc cggggtgcgc gcagatcaca tccggcacga agccgccctt gcgcagctcg
      241 aggcaggtgc gcaccaccgc ctggccgcgc cgcaccgcgc cctcgatgcc ctgcaggtag
      301 aggtgcgtag tcgggttcgc gcccttcggc ttgtcgtacc agaggtggcg catgcccttg
      361 aggctgacct ggcgctcgtc cttgcgctcg ccgatcgaca ccacctggtg cttcgggttg
      421 cgcgcgaagt gcgccgccag gtgcttgtac tggccgggga agttctggtg gacgaacagg
      481 atcttcatgg cttcttcttg gcgccgaggt agcggttgtc gaggtgccgc acctcgagga
      541 agccggcgtt ctccggcgcc ggcttgtagg tgcggaagtg gcgcaggatg ccgaagctcg
      601 ccgcgctctc gagcgggccg acgaagctcg gctcgcgcgc gaggaagccc ggctgctgcg
      661 tccagcgttc catctggcgc gcgttcagga agaagcagcc cgagtgcggg ttggcggcgc
      721 gcacgaagcg cacgcgcacg ccgaacgcct cggcctcgat cggcgggcgg tcgttcacgt
      781 cctgccagcg cgtgctccac tcggggcgcg agagctcgcc gtcgacgaac agcttgtgcc
      841 agcgctggcc gatggcgatc tcgttgcggt tgggctgcag caccgcctcg tcgccggcga
      901 ggcggttgaa ccactcgagc ttgtggaaga acagcgggtc gagcagcgcg atgtcgtcct
      961 cgaggtagca gaaccagtcg aagcgcccga gtccctggcg cagcacctcg tggcactcga
     1021 agccgaggta catcggctgc gcctgcgtcg cgtggtggcg gaagctgtcc ggcggcaggc
     1081 gcatctcgtc gagcaggtgg cgcccgccgg tggtgcacac caccacctcg aggtcggcgg
     1141 tggcggcggc gttgcacgcc acggtcggcg cgccgaggaa cgcctgctgc cggccgaggt
     1201 tggcgtgcag gctgaagatc gcgccggcga gcgcgttgcg ccgcggcgcc gagtcggcct
     1261 tgagcgagcc gtatttgccg ccgcccgccg ggtcgtagta gtgcggaatg gtgaacagga
     1321 tgcgcatgac gggctagcga acctcgaagc tgaagcggat cggcggcggc gcgcggccgg
     1381 ccggtgcggt tttttcggcg gcaatggtaa cccaccaggt gccgctcgcc caaggctcgg
     1441 cgccgcgctt gcgcccgacg gcgcgataca cccgggccag gggccggtcc aaaacaattt
     1501 tctgttcaaa tcgggtcgtg ccgtccggcg cctcgacccg cacgcgcagc gtctcgccgc
     1561 cggtggtgcc aaacgcctcg gcccacacca tcagggccgg cgcgtcgcgc gcgagttcgc
     1621 gcgcgcggta ggcgccgctg cgcacctcgc tctccggtgg aatgcgcgcc gccacgccat
     1681 agttgtagat cgcaggcggc gcgtagccga gcgcgtcgag cgtcgccgcg tcccacagcg
     1741 ggcgctcgcc cgggccgcac gccgggccgg cgccttcgcc gcggaacgga tcgagcaccg
     1801 cgccgccgcg ccgcaccgtg aaatgcaggt gcggatattc ggtcaggccg gaaaggccga
     1861 ccagcccgag cggctggccg gcgcgcacgc gctcgccgcg cttcaccgcc acgctgccgc
     1921 ggcgcaggtg gcaatactgg gtctgcagac cgccgccgtg ctcgatgcgc acggcgttgc
     1981 cgcactcgat gcccgcgatc gcgtcgcggc cgcgcgcgcg cacgctcacg tcctcgacgc
     2041 cgtcgcgcgt cgcgcgcacc acgccgccgg ccgcggccag caccttcacg ccctggtcca
     2101 tcgccgcggc gtcgcgcagc gcgatgtcgg tgccgttgtg gtcgttgtag gtgaaggtgc
     2161 cgcaggcgta gtcgcgcgcc tgcggcccgg ggtcgaggtc gacgtggttc gagatccagc
     2221 agtcggcgcc cggcgtgcat tggagcggta gttgcaactg ctgggacgcg gccgcgccgg
     2281 cgaaaagcac aagccatgca gcaataccga tcttgttcac ggcgccgttg tagcatgccg
     2341 ccgtgcgccc gccccagcgt tccgacaacg ttacgcaagc cctgcagacc gcgcgcgagc
     2401 acctgcgcgc cggccgccac gccgaggccg aggcgtgcct ggcgccgctg gtcaaggtga
     2461 acccgaaaca tgccgccgcg ctgcagctcc tcggcgtgat cgcgctcgcg cgccgcgact
     2521 tcgccgccgc cgcggatttt ctcgagaagt cgctcgccgc cgaccccagg caggtgcagg
     2581 cctggaacaa ctacggcgcg gcctgccagg cgctcggccg cctcgagcag gcgatcggcg
     2641 ccttccggcg cgcgctcgag ctgcagcccg gccacgccgg cgcgctgaac aacctgggcg
     2701 tgctgcacgc gcggcgcaac gagctcaccg aggccgaggc c
//
LOCUS       DASRIE010000075         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1646305, whole genome shotgun sequence.
ACCESSION   DASRIE010000075 DASRIE010000000
VERSION     DASRIE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646305"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFB53_03540"
     CDS             <1..254
                     /locus_tag="VFB53_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN85838.1"
                     /translation="IEAQAAGGRLSALQRAFHEHLGAQCGYCTPGMVMAAEALLRSTP
                     RPTEAQIREALAGNLCRCTGYVKIIESVQAAAAALEDGR"
     gene            251..>2052
                     /locus_tag="VFB53_03545"
     CDS             251..>2052
                     /locus_tag="VFB53_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN85839.1"
                     /translation="MKNANPAARTVAPPTHSIGVRTPLVDGVEKVTGRARYTADLPLG
                     PVLTGAIGRSPLAHARIVAIDTAAALALPGVRAVITGEDFHAPYGVIPISQNEWPLAR
                     GKVRYRGEPLFAVAADDPQTALAALAAVRVELQELPAYFTAAAAREPGAGLLHEDKPG
                     NLERMVEQEFGDMAAGFAAADLVLERRFECAEIAHGQLELDASVAQWDPEAQRLTMHS
                     VTQVPYYLHLMLAQTLGLAESQIRVVKPFVGGGFGHRVEALNFEMVAAALARAAGGTV
                     KLELTREEVFLTHRGRPHSEIALRVGLRKNGEITALDAEVVQRGGAYGGYGLVTILYA
                     GALLHALYRVGAVRYRGWRVYTNLPPCGAMRGHGAVNVRFAVESLLDEAAAALALDPF
                     ALRRANLITPPWRTPNDLQVNSCGLPECLDAVEQASGWRERRGRLPAGVGLGMACSHY
                     VSGSAKPVHWTGEPHAVVNLKLDFDGSITVLTGAADIGQGSTTLLAQVVAEVLLLPMA
                     RIRVLCTDSALTPKDNGSYSSRVSFMVGNAALRAAEALRRVLLEAAAQRLAQPLEALE
                     WLGECCAVSGSEQRLSFDDCVQAALVDEGTLTVKG"
BASE COUNT          275 a          742 c          753 g          282 t
ORIGIN      
        1 ccatcgaggc gcaggccgcc ggcggtcggc tgagcgcgct gcagcgcgcc ttccacgaac
       61 acctgggcgc gcagtgcggc tactgcacgc cgggcatggt gatggccgcg gaggcgctgc
      121 tgcgcagcac gccacgcccc accgaggcgc agatccgcga ggcgctggcc ggcaacctgt
      181 gccgctgcac gggctacgtg aagatcatcg agtcggtgca ggccgcggcc gcggcgctgg
      241 aggacgggcg atgaagaacg ccaacccggc cgcgcggacg gttgcgccgc ccacgcactc
      301 gatcggcgtt cgcacccccc tggtcgacgg cgtcgagaag gtcacgggcc gcgcccgcta
      361 caccgcggac ctgcccctgg ggcccgtgct caccggcgcg atcggccgca gcccgctggc
      421 gcacgcgcgc atcgtcgcga tcgacaccgc cgccgcactc gcgctgccgg gtgtgcgcgc
      481 cgtgatcacc ggggaggact tccacgcgcc ctatggcgtg atcccgatct cgcagaacga
      541 gtggccgctg gcgcgcggca aggtgcgcta ccgcggtgag ccgctgttcg ccgtggccgc
      601 cgacgatccc cagaccgcgc tcgctgccct ggccgcggtc cgggtggagc tgcaggagct
      661 gcccgcgtac ttcaccgcgg ccgcggcgcg cgagcccggt gccggcctgc tgcacgagga
      721 caagccgggc aacctggagc gcatggtgga gcaggagttc ggcgacatgg cggccggctt
      781 cgccgcggcc gacctggtgc tggaacgccg cttcgaatgc gcggagatcg cgcacggcca
      841 gctggagctg gacgcctcgg tcgcgcagtg ggaccccgaa gcgcagcgcc tcaccatgca
      901 ttcggtgacg caggttccct actacctgca cctgatgctg gcgcagacgc tcggcctggc
      961 ggaatcgcag atccgggtgg tgaagccctt cgtgggcggc ggcttcggcc accgggtgga
     1021 ggcgctcaac ttcgagatgg tcgccgcggc gctggcccgc gcggccggcg gcacggtgaa
     1081 gctggaactc acgcgcgagg aggtcttcct cacgcaccgc gggcgcccgc attcggagat
     1141 cgccctgcgg gtcggcctgc gtaagaacgg cgagatcacc gcgctggacg cggaagtcgt
     1201 gcagcgcggc ggcgcctatg gcggctacgg cctggtgacc atcctttacg cgggggcgct
     1261 gctgcacgcg ctgtatcgcg tgggcgcggt gcgctaccgc ggctggcgcg tctacaccaa
     1321 cctgccgccc tgcggcgcca tgcgcgggca cggcgcggtc aacgtccgct tcgccgtgga
     1381 atcgctgctc gacgaagcgg ccgcagcgct ggcactcgat ccgttcgcgc tgcgccgcgc
     1441 caacctgatc accccgccct ggcgcacgcc caacgacctg caggtcaatt cctgcggcct
     1501 gcccgagtgc ctggatgcgg tggagcaggc ttcgggctgg cgcgagcgcc gcggccggct
     1561 gccggctggc gtcggcctcg gcatggcctg ctcgcactac gtcagcggct cggccaagcc
     1621 cgtgcactgg acgggcgagc cgcacgcggt ggtcaacctc aagctggatt tcgacggcag
     1681 catcacggtg ctcaccggcg ccgccgacat cggacaaggc tcgacgacgc tgctcgcgca
     1741 ggtggtcgcc gaggtgctgc tgctgcccat ggcgcgcatc cgcgtgctgt gtaccgacag
     1801 cgcgctcacg cccaaggaca acggctccta ttcctcccgg gtgtccttca tggtgggcaa
     1861 cgcggcgctg cgcgccgccg aagccctgcg ccgcgtgctg ctggaggccg cggcgcagcg
     1921 cctcgcgcaa ccgctggagg cgctggaatg gctgggggaa tgctgcgccg tctccggcag
     1981 cgagcagcgc ctgtcgttcg acgattgcgt gcaggccgca ctggtcgacg aggggacgct
     2041 cacggtcaag gg
//
LOCUS       DASRIE010000076        20959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_603391, whole genome shotgun sequence.
ACCESSION   DASRIE010000076 DASRIE010000000
VERSION     DASRIE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603391"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFB53_03550"
     CDS             <1..1219
                     /locus_tag="VFB53_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85840.1"
                     /translation="AHQAQLHGALQRAYDDLHQTQQVVLQQERLRALGQMASGIAHDI
                     NNAVSPVALYTEALLEKEPGLSADARAKLQTIQRAIDDVAETVARMREFYRPQDALSR
                     QLPVNLNTLVRQVLDLTRARWRDMPQQAGIAIEVVTLLDDDLPPVLAAEGEIREALTN
                     LVFNAVDAMPTGGTLTLQTLQRHDPGGVTTVQVEVIDTGGGMDEETRRRCLEPFFTTK
                     GDRGTGLGLAMVYGTVQRHGGDIEIKSAPGQGTTVRLLFPAAGQPVPRESAWGQLETG
                     HAAVSLSLLLIDDDPLLRRSLTDILESEGHAVTAASGGQQGIDAFTAAAARGTPFDVV
                     ITDLGMPNVDGRQVAYAVKSVRPGTPVMMLTGWGRRMREDGELPPHVDHLLSKPPRVA
                     ELRSALARFAPAP"
     gene            1234..2343
                     /locus_tag="VFB53_03555"
     CDS             1234..2343
                     /locus_tag="VFB53_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN85841.1"
                     /translation="MDARPRLLVVDDEILQLDALSQVLREEGYEVRACTQPHEALQAL
                     REGGFDVLLCDLNMPGMDGIALTREALAIDADLVPVLMTGQGSIPTAIEAMKVGALDY
                     VLKPFRMAAMRPVLVRAAEMRKLRVGNRRLKEDLERRNVQLVAANRELDAFAARIAHD
                     LRGPVMGMLGFARILHERGAAGLTQEQRGFLQRVVSAGERAERMIRDLLNFARLGEST
                     MRREPVSLDDVVLAARKAVQGDEEGRHVDWHIGRLPTVHGDEALLELVFVNLLSNALK
                     YTRPRAHASVEIGSCAAPEGGHVVWVRDNGVGFDPSHANRLFTPFQRLHSAQQFEGQG
                     IGLANVKRIVERHGGAVAAEGAPGAGATITVTLPA"
     regulatory      2404..2489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2615..4777
                     /locus_tag="VFB53_03560"
     CDS             2615..4777
                     /locus_tag="VFB53_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN85842.1"
                     /translation="MRQPSTIPFVILIESRIVRALLGMLAAALVFAGLCLPGDVRAAA
                     ADKIAADLARAIQGASAPGPRWVREVAGVRHVKAIVLASSSDAQLADLRAHVLAVGGS
                     VYARYVSVPGLSVMLPAHRVAEIAARPDVLGISPNRPTRRTASALELATGAAGVRGAA
                     AQGAADGLDGSGIGIAVLDSGIASAHYNFRDAQLRHSRVAGAMDFVSAGSAMAAGARD
                     WIAGVDVSASRRGVALPAGADPYGHGSHVAAVAAGRGDHRALDSTGIAPNASLYDVRV
                     LDSAGTGELGDVLAAIDWVIQQARVANIRVLNVSLAADSTESFLTDPFCRAVRSATAA
                     GITVVVAAGNYGQGADGAERYGTISSPGHEPSAITVGAANTRETARRDDDVVNRFSSR
                     GPTRGAWVDAAGVRRIDNLLKPDLVAPGNRIVGVLAAGAHGSERFLSALAERHPELSL
                     PYGGTAQPRLQQLMNLSGTSIAAPAVAGTVALMLQKNPGLTPPLVKAILQYTAQPLPG
                     ASLLQQGTGLLNADGAVRLAGAIRTDVSAALAAGTLKPGDPLLAPGKALPAPTSTLEG
                     MAVSWSRMIVAGGTHVLGGPALFTQFQPIYDPRLLWVRDAAWRLDRGAEVPVRERRLV
                     TGGVVRALPSASSLRERTGAFVPVPALSGWLATGGGIVMSEGIVMSEGIVMSEGIVMS
                     EGIVMSEGIVMSEGIVMSEGIVMSEGIVMSEDASLGEP"
     gene            4774..6444
                     /locus_tag="VFB53_03565"
     CDS             4774..6444
                     /locus_tag="VFB53_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN85843.1"
                     /translation="MNATSAAGVAAPPRAAGTGWLPLHQRLMPDYNRPAALYWWAVVL
                     LGLAVLLHSLAHLAGVPPAAWVHVAAGTLLAMLAGLVPVRIPRSTNSFTAGEIFIFLL
                     LLLDGAQAAALASACEALVGSWRSSKRWTSRIASPAMAALAMFTAGSALHAALDALRR
                     HGMAADGVLIAAAVCFAIAYFVLNTVMVTMVGRLKRNEPLRLRAFVDSFGWLGVAYAG
                     NASVATFLYLSFRSSGIAVVVAAVPIIVILLVTGHFYFRREEAEEAVRKAREQQAAQR
                     QMLENARQAGMAEIATNVLHNVGNVLTSVNVSAELICNRVRASEAAGLPRAVRLLDEH
                     AHDLGEFLTRDAKGRLLPEYLRHLAQALMEERGGLLQELESLTLSVKHIKEIVATQQS
                     YAGASSVVEAIQVRDLLEDALRINTVGAGDRAPAVVREFADVPQAMLDKHRVLLILVN
                     LIRNATQAMAGMVHVPHVLRLSLGVTEKALLIVVEDSGEGIAPENLTRIFAHGFTTKK
                     DGHGFGLHSCALAAREMGGALTAHSDGPGRGARFTLELPFNIPPETTA"
     gene            6441..7853
                     /locus_tag="VFB53_03570"
     CDS             6441..7853
                     /locus_tag="VFB53_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85844.1"
                     /translation="MNVANRRILLVDDMPTIHEDFRKILAPPTTASAALLAAKAALFG
                     DDTPAPAVNFELDSAFQGQEGLAKVEASLQAGLPYAMAFVDMRMPPGWDGAETVARLW
                     QADPKLQIVICTAYSDSPWEEVLERLDARDRLLILKKPFDNIEVRQLANALTAKWALT
                     QQAALHIDRLEDAVQERTRKISDANEALRAEIAERKHLEGQLVQSEKLASIGQLAAGV
                     AHEINNPIGYIFSNFNTLDSYLGDLFEVLGAYEAAEPSMAPPSVAAGLATLRAQRQLD
                     FLKEDVPGLMRESKEGIARVRAIVQDLKDFSRVDSAQEWQRASLHQGIDSTLNVVASE
                     VRYKADVVKEYGQVPEIECLPSQINQVVMNLVVNAAHAMGPERGRITIRTGAEGDQAW
                     FEVADTGCGIPPENLSRIFDPFFTTKPIGKGTGLGLSLSYGIVQKHRGRIDVCSERGR
                     GTTFRVVLPVRHAAEEAALP"
     gene            7850..9202
                     /locus_tag="VFB53_03575"
     CDS             7850..9202
                     /locus_tag="VFB53_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN85845.1"
                     /translation="MNGVSIIEAAAGRRILLVDDEPNVLSALRRSLRGRGFDIATAGN
                     GEQALAELRREPVDAIVCDMRMPGMSGAEVLKASIALAPDAVRVLLTGYADVESTVRA
                     VNEGEIFRYLAKPWDDAFLLQVLDDGLARKALERERDALLALTEQQNRELRTFNARLE
                     AQVAQRTQELQASMAELRKATERLKDDFAGTVRLLSGLVQGRAGLTARCPSAVARHVR
                     AFAPRLDLAGEALHDLTFAALLQDLGKVALPDALVRQPFEALDTPARQLLLNHPQIGR
                     ISMASLASLRGAGEILGSVQENFDGSGVPGKASGEDIPLGARALRVASDFEQYLAGAL
                     TLEPLSPEQAFRRLRQSRGTRYDPRAVDAFLAYMEQPVTGPARKVLMSSANVRVGMRL
                     AQDLVTGAGSLLLARGYVLDEGVIAQLRRLEEFSGEFLWIAVSSDDGVEVRVGEAVAP
                     "
     gene            9199..11652
                     /locus_tag="VFB53_03580"
     CDS             9199..11652
                     /locus_tag="VFB53_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN85846.1"
                     /translation="MTAAECGDPVRAPVVLQPLLDALDHGADTLVVTCAELRVRYANR
                     AARRLLGEAVAPGAPFTLGARATVAGFAHLTLADLQGDARAIELRVSSGDWLGEPALF
                     HALRDVTQRRAAEQGLVLGRQAMEASANGIMIVDAGDPQQPIIYVNPAFERITGYRRG
                     EAIGRNGRFLQGHERDQPSLRALREALAAGTDVSVVLRNYRKDGTPFWNELSVSPVRD
                     AAGRVTHFVGIQNDVTERLRQEAELVRRSTHDELTDLPNRSVLLERLRQALRERGRAH
                     GALALLLVDLDRFKAINDALGHDAGDEVLFETAARLRGCLGDRDTLARLNGDQFVALL
                     PAREGDAAVNDVAQAMLRAVAEPYQVAGQTVFCTCSVGIAFAGDAQEEPAQLLQCADL
                     AVHAAKRGGRNAAHAYSADMVQRASDRMAMRNALHDAIQRGELHLHYQPQLDVRSQRI
                     CGVEALVRWRHPTLGPLAPADFIPLAEETGQIVALGEWVMARACEQHRQWLDRGLIDG
                     PVAVNVSVVQFRRAGFVDRVAEILARTGLPPGRLELELTESVMMERGECSFEALHRLR
                     ALGVRLSIDDFGTGFSSLGYLKRLPIDKIKIDRSFVRDITSDGDDAAITLGIIAMAHH
                     LRMRVIAEGVETPAQLAYLRRHLCDEAQGFHVSAPLPPAALEAFLAAFAPPAVETAAG
                     DDAAPPTLLLVDDEPNILSALTRTLRRDGYRIFTAGGADQAFQVLAQQEVQVILSDQR
                     MPAMCGTDFLSEVKRLYPDTVRMVLSGYTDLQSVTEAINRGAIYRFLTKPWEDEPLRA
                     HIREAFAHHQRERLGAALA"
     gene            11812..12921
                     /locus_tag="VFB53_03585"
     CDS             11812..12921
                     /locus_tag="VFB53_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN85847.1"
                     /translation="MSQWPGRLRVLGIDDEADDLELIRLALQGGGVDADVIPVWDEDG
                     LRRALEPAPDLVLCDYSMPGFSAERAMAVLAERAPHLPLVVVTRAIGEEAVVGLFRAG
                     AKDYVGKDKLGLLPGVVRRVLSERSMALERERAAQALASANTRLRLLSSHLVDAQERE
                     RTRIARELHDGLGQLLTGVVIQLQAAGNSQVPEQAERCRRWALELAEQGIRQVKTMSF
                     QLRPPQFELLGFAAAVQATLDRQLEATCVQGGVRLRGVDPHVAGAAQVVALRVLQEAL
                     TNVLRHAGARRVLVRVRFMDANRFVLTLGDDGCGFDVKAALGEGLSERNLGLHGMLER
                     AELMGGRLRFRSSPGRGTVVRLVSSADEAATPRWT"
     gene            12912..13568
                     /locus_tag="VFB53_03590"
     CDS             12912..13568
                     /locus_tag="VFB53_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN85848.1"
                     /translation="MDMSGAQEIRTVLADDHELVRAGLTLLLERVPRVRVVGQASSGE
                     ELLAVARRERPALVVTDISMPGMNGLAAIGELRALPDPPRILVVSMHDSPDFIRRAMT
                     LGASGYVMKEGSAVELEQAVRRVMGGHTYFSPQVSQKMMQRPDPAPEEVLTERQLEIL
                     VLIASGHASKEAAFKLGLSPKTVDVHRARIMERLGIDDVAGLTLYCVRHGLVDPRRAE
                     "
     gene            complement(13665..14309)
                     /locus_tag="VFB53_03595"
     CDS             complement(13665..14309)
                     /locus_tag="VFB53_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85849.1"
                     /translation="MRRIVASISAVLMLAAVTAVAQEKAKDQPKSGHEHMAKAGGARS
                     DAAVIAKATSAAPPDIGRNAAVMGTGADGKMKELRAGTNGWMCMLDLVGDSMCLDKEW
                     QAWGDAWMNKKDPPKPKSVGVAYMLKGDKGASNTDPFATKPTADNQWVVSGPHIMILP
                     TDVGQLEAYPTDWTKGGPWVMWKGTPYAHIMVPTTSMPKSASKAPAEKKSSDKK"
     gene            complement(14448..15161)
                     /locus_tag="VFB53_03600"
     CDS             complement(14448..15161)
                     /locus_tag="VFB53_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN85850.1"
                     /translation="MNSKLSNQDIYDRIYSAISERRLLPGTKLSEERLAQAFRASRTR
                     VREVLLRLGQELIVESHPNRGAFVASPTTEDMRHVFAVRRALERAIAAELSERCGGQP
                     IAPLRAHLESEARARGEGDRAALARLTGDFHVRLAEATGNRIFTENVRRLVALTGLII
                     AQYDAQASSACPENEHADIVKAIEAGDARRAERLMLQHLDHVESGIEAPAAGAGEVNF
                     ERIFGTVEPPAPKKRARRA"
     gene            complement(15187..15990)
                     /locus_tag="VFB53_03605"
     CDS             complement(15187..15990)
                     /locus_tag="VFB53_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85851.1"
                     /translation="MTELLEKDARPPPAAREAAAERRAREPAHLELAGVSLRYGEVRA
                     LEQVSLVVPRGAIVALVGANGAGKSSTLRAITGLRRVATGEVRLEGRVVNSPGRQEPA
                     HALVRQGVAMVPEGRHVFPYMSIKDNLLMGAYTRHDKAGIAADLDKVCERFPRLKERF
                     HQQGSSLSGGEQQMVAIGRALMARPKLLLLDEPSLGIAPQVIRLIGRSLLEINRNDGI
                     TVVLVEQNSRMALALSSYAYVLETGRVALEGPSKDLTTNEHVKRLYLGG"
     gene            complement(15987..16814)
                     /locus_tag="VFB53_03610"
     CDS             complement(15987..16814)
                     /locus_tag="VFB53_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85852.1"
                     /translation="MKPRDVPLEATAGLAQAVLEARGITKRFGGLVANEHVDLVLTGP
                     AGQVVSVIGPNGAGKSTFFKMLSGFLKPSSGSVLLHGQDITGLPPHRIARLGLVRTFQ
                     ETTIFPDLTALEHVSLAHQLHREANDLQVVLGTRGARADEDRLAASARAILALMELSP
                     AADVAARHLPQGLLRLLGIAMGLAARPRVLLLDEPYAGLNPEETDRAVAITRKIAASG
                     VSVLLVEHDMRAVMAISDRIHVLHFGRKIAEGTPAEIRDDPKVIEAYLGQEDEELGL"
     gene            complement(16811..17743)
                     /locus_tag="VFB53_03615"
     CDS             complement(16811..17743)
                     /locus_tag="VFB53_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN85853.1"
                     /translation="MNPLKKRDLAIALCFLAALAALPLAIGPSRYLLSVLMNCAGLSV
                     VAFGVWLTFTIGRINICQAGFALIGGYTTAIVLSRHGMSFWLALPLSALASAALGAII
                     GSFILKLRGIYFSMLTICLTEAIRLAFLNGGEFTQGSRGITGLPQPFAGESPLPMYYL
                     AIALLGVALLVTWRVHYSRVGRIFRAMRLNEDLAESFGTDVWRYRVTAFAIACALGGL
                     GGSYFAVFTQSVYPQSFTVEHSIYYMLYCFLGGLEFVSGAMIGAFVLTLLFEVLSQFQ
                     QYQTLVYGVLMIGVILLLPNGLMAIRARALEAKA"
     gene            complement(17793..18668)
                     /locus_tag="VFB53_03620"
     CDS             complement(17793..18668)
                     /locus_tag="VFB53_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN85854.1"
                     /translation="MIQVLINGAVLGCVYALVALGLTMVFSIMRVVNFAHGQMYMLGA
                     FVVYYLYGEYGLPFYVALPAAAAALALVGVAFNRFFFQPVLASATREENSMLMAVGTA
                     LLLENLALTVFGEKQRGVPPVVDGAFVFGDVAEGGAILTHPNAMVAALAALSILGLLA
                     FVQYTRPGRALRAVAQDKEAARLQGVDVKATTTLGFAIGAGLAGLAGGLLVTVYGVNS
                     GVGVNISTKAFIMVMIGGAGVTSGAIVGGVLLGFAEAIGYDLLPGSITYLLIFVAMIV
                     FLVFKPNGLFGKPWG"
     gene            complement(18694..19887)
                     /locus_tag="VFB53_03625"
     CDS             complement(18694..19887)
                     /locus_tag="VFB53_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85855.1"
                     /translation="MNHRIRLHLTLLAAATPLALFGAPSRAQEVLRIGVNGVMSGEAA
                     SWGLVNKYCAETTADMVNAKGGADIGGKRYRIEIVALDDKNDPKISVTNAEKLTSQGI
                     KYIIGPNIDTTALAVKPVVERAKALNFPYAFAKELYKAPAGNSVLGMVASYQVGPIMY
                     EYLKTKKGVKSIAFLARNESDARNQQAEGVEAAKKLGLNVVAASDSYEPGTKDFMPVM
                     TKVIKTKPDAIVLSGVAPADAPLLNKAARDLGFKGTISTETAQDSKILAQVAGAKANG
                     FMSVGGASTPEIQSDYMKEFVSVYKKRVGEWNDEAGTKVYALELILAVLKKNPKALDD
                     VEVFKKEMATFSMPNPFVKGGATRLEFAGQADFGRRSQVGVPLVINEFQDKEFKTLFV
                     GTIRN"
     gene            20072..20806
                     /locus_tag="VFB53_03630"
     CDS             20072..20806
                     /locus_tag="VFB53_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZN85856.1"
                     /translation="MRIQVINPNTTRSMTERIGAAARFVAGPGVTIEAVNPSQGPASI
                     EGHYDEALACVGLLHEVRQGVVAGVDAHVIACFGDPGLDAAREIARGPVIGIAEAAMR
                     SACYLATGFSVVTTLKRTQVIAEHLVDKYGVRPYCKRVRACEIPVLELEDPQSDAYRT
                     ILAECRRALAEDDSAAIVLGCAGMADLCERLQAELGVPVVDGVAAATVTAQALVRARL
                     ATSKRGDYARPLPKPYAGALAAFAIE"
     gene            20810..>20959
                     /locus_tag="VFB53_03635"
     CDS             20810..>20959
                     /locus_tag="VFB53_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HZN85857.1"
                     /translation="MSYPRDLRGHGRHPPHARWPGNARIALQFVLNYEEGGENSVLHG
                     DAGSEQ"
BASE COUNT         3015 a         7381 c         7543 g         3020 t
ORIGIN      
        1 cgcccaccag gcgcagctgc acggcgcgct gcagcgtgcc tacgacgact tgcaccagac
       61 gcagcaggtg gtgctgcagc aggagcggct gcgcgcgctc ggccagatgg ccagcggcat
      121 cgcgcacgac atcaacaacg ccgtctcgcc cgtcgccctg tacaccgagg cgctgctgga
      181 gaaggagccg ggcctgagcg ccgacgcgcg cgccaagctg cagaccatcc agcgcgccat
      241 cgatgacgtc gccgagacgg tggcgcgcat gcgcgagttc taccggccgc aggacgcgct
      301 gtcgcggcag ctgccggtga acctcaacac cctggtgcgc caggtcttgg acctgacgcg
      361 ggcgcgctgg cgcgacatgc cgcagcaggc cggcatcgcg atcgaggtgg tgaccctgct
      421 cgacgacgac ttgccgccgg tgctcgcggc cgaaggcgag atccgcgagg cgctcaccaa
      481 cctggtgttc aacgccgtcg atgcgatgcc gaccggcggg acgctcacgc tgcagacgct
      541 gcagcggcac gacccgggcg gcgtcaccac ggtgcaggtg gaggtgatcg acaccggggg
      601 cgggatggac gaggagacgc ggcgccgctg cctggagccc ttcttcacca ccaagggcga
      661 ccgcggcacg gggctcgggc tggcgatggt ctacggcacg gtgcagcgcc atggcggcga
      721 catcgagatc aagagcgcgc cggggcaggg cacgacggtg cgcctcctgt tcccggcggc
      781 cggccagccc gtgccgcgcg agtccgcctg gggccagctc gagaccggtc acgccgcggt
      841 gtcgctcagc ctgctgctga tcgacgacga tccgctgctg cggcgctcgc tcaccgacat
      901 cctggagtcc gagggccacg cggtgaccgc cgcgtccggc gggcagcagg gcatcgatgc
      961 gttcaccgcg gcggccgcgc gcggcacgcc gttcgacgtc gtgatcaccg acctcggcat
     1021 gccgaacgtc gacggccggc aggtggcgta cgccgtcaag tccgtgcggc cgggaacccc
     1081 ggtgatgatg ctcaccggct ggggacggcg catgcgcgag gacggggagc tgccgcccca
     1141 cgtcgaccac ctgctgagca agccgccgcg cgtggccgaa ctgcgcagcg cgctcgcgcg
     1201 cttcgccccc gcgccatgaa gggagaacaa gaaatggatg cacggccccg cctgctggtc
     1261 gtggacgacg agatcctcca gctcgacgcg ctctcgcagg tgctgcgcga ggaaggctac
     1321 gaggtgaggg cctgcacgca gccgcacgag gcgctgcagg cgctgcgcga gggcggcttc
     1381 gacgtcctgc tgtgcgacct gaacatgccg ggcatggacg gcatcgcgct gacgcgcgaa
     1441 gcgctggcca tcgatgccga cctggtgccg gtgctgatga ccggccaggg ctcgatcccg
     1501 acggccatcg aggcgatgaa ggtgggcgcg ctcgactacg tgctcaagcc gttccggatg
     1561 gctgccatgc ggcccgtgct cgtgcgcgcc gccgagatgc gcaagctgcg cgtgggcaac
     1621 cgccggctga aggaagacct cgagcggcgc aacgtgcagc tcgtggcggc caaccgggag
     1681 ctggacgcgt tcgccgcccg catcgcccac gacctgcgcg gcccggtcat ggggatgctg
     1741 ggctttgcgc gcatcctgca cgagcgcggc gccgccggcc tgacgcagga gcagcgcggc
     1801 ttcctgcagc gcgtcgtgag cgcgggcgag cgcgccgaac gcatgatccg cgatctgctg
     1861 aacttcgcgc ggctgggcga gagcacgatg cgccgggagc cggtctcgct ggacgacgtc
     1921 gtcctggcgg cgcgaaaggc ggtgcagggc gacgaggagg ggcgccacgt cgattggcac
     1981 atcgggcggc tgcccaccgt gcacggcgac gaggcgttgc tcgagctggt gttcgtcaac
     2041 ctgctgtcca atgcactgaa gtacacgcgg ccgcgggcgc acgcatccgt cgagatcggc
     2101 agctgcgccg ccccggaggg cggccacgtg gtgtgggtgc gggacaacgg cgtcggcttc
     2161 gaccccagcc atgccaaccg gctcttcacg ccgttccagc ggctgcacag cgcccagcag
     2221 ttcgaaggcc aggggatcgg cctggccaac gtcaagcgca tcgtcgaacg gcacggcggc
     2281 gcggtcgcgg cggagggcgc gccgggcgcg ggtgcgacga tcacggtcac gctgcctgca
     2341 tgagacgccg taggcaattt tcttaaccgc ccggcgccgg tgcccgatac attcggttcc
     2401 acctgatgac aaaggcacac ccgcgcgaaa gccggggtcg caaagcttcc ggtcttcggc
     2461 ctcacggcca tgacagcggg gttgccagca gacgcacggt cttcgaggcc gctcgtcccc
     2521 cgtcggtgcc caccggcgac ggcaacgctt ccccgcggac ggtgtcgcga cttccctgcg
     2581 cgcgcgtgtt cctccacgcg cagacggagc ttccatgcga cagccatcca ccatcccctt
     2641 cgtgatcctc atcgagtcgc gcatcgtgcg cgcgctgctc ggcatgctcg ccgccgccct
     2701 ggtgttcgcc gggctgtgcc tgccgggcga cgtgcgcgcc gcggccgccg acaagatcgc
     2761 cgccgatctc gcgcgcgcca tccagggcgc gagcgcaccc ggcccgcgct gggtgcgcga
     2821 ggtcgccggc gtgcgccacg tcaaggcgat cgtgctggcc agttcgtccg acgcccagtt
     2881 ggcggatctg cgggcccacg tgctggccgt cggcggctcg gtgtacgcgc gctacgtgtc
     2941 cgtgccgggc ctgtcggtga tgctgccggc gcaccgggtg gccgagatcg ccgcgcgccc
     3001 cgacgtgctc ggcatctcgc ccaaccggcc cacccggcgc acggccagcg cgctggaact
     3061 ggccaccggc gcggccggcg tgcgcggcgc cgcggcccag ggcgcggcgg acggcctcga
     3121 cggcagcggg atcggcatcg cggtgctcga ttcgggcatc gcctcggcgc actacaactt
     3181 tcgcgacgcg cagctgcggc attcgcgggt cgccggcgcg atggacttcg tgagcgcggg
     3241 cagcgccatg gccgccggcg cgcgcgactg gatcgcgggg gtcgacgtgt cggcctcgcg
     3301 ccgcggcgtc gcgcttccgg cgggcgccga tccctacggg cacggcagcc acgtggcggc
     3361 cgtcgccgcc ggccgcggcg accaccgggc gctggattcg accggcatcg cgcccaacgc
     3421 ctccctgtac gacgtgcgcg tgctggacag cgcgggcacc ggcgagctcg gcgacgtgct
     3481 ggccgcgatc gactgggtga tccagcaggc gcgggtcgcc aacatccgcg tgctgaacgt
     3541 gagcctggcc gccgactcga cggagtcgtt cctcaccgac ccgttctgcc gcgcggtgcg
     3601 cagcgcgacg gcggccggca tcacggtggt ggtggcggcc ggcaactacg gccagggcgc
     3661 ggacggcgcc gagcgctacg gcacgatcag ttcgccgggc cacgagccct cggcgatcac
     3721 cgtgggcgcg gccaacaccc gggagaccgc gcgccgcgac gacgacgtcg tgaaccgctt
     3781 cagctcgcgc ggcccgacgc gcggcgcctg ggtcgacgcg gcgggcgtgc gtcgcatcga
     3841 caacctgctg aagcccgacc tggtggctcc cggcaaccgc atcgtcggcg tgctggccgc
     3901 cggggcgcac ggatcggagc gcttcctgag cgcgctggcg gaacgccatc ccgagctgtc
     3961 gctgccgtac ggcggaacgg cacagccgcg gctgcagcag ctcatgaacc tgagcggcac
     4021 gtcgatcgcc gcgccggcgg tggccggcac ggtggcgctg atgctgcaga agaacccggg
     4081 cctcaccccg ccgctggtga aggcgatcct gcagtacacc gcgcagccgc tgccgggcgc
     4141 cagcctgctg cagcagggca cgggcctgct caacgccgac ggggccgtgc ggctggccgg
     4201 cgcgatccgc accgacgtgt cggccgccct cgccgccggc acgctgaagc cgggcgaccc
     4261 gctgctggcc cccggcaagg cgctgccggc gccgacgtcg accctcgaag gcatggcggt
     4321 gagctggagc cgcatgatcg tcgcgggcgg cacccacgtg ctcggcggac cggcgctgtt
     4381 cacgcagttc cagccgatct acgacccgcg cctgctgtgg gtgcgcgacg cggcgtggcg
     4441 cctcgaccgt ggcgccgagg tgccggtgcg cgagcgtcgc ctggtcaccg gcggcgtggt
     4501 gcgggcgctg cccagcgcga gctcgctgcg cgagcgcacg ggcgccttcg tgccggtgcc
     4561 ggccctctcc ggctggctcg ccaccggcgg cggcatcgtg atgagcgagg gcatcgtcat
     4621 gagcgagggc atcgtgatga gcgaaggcat cgtcatgagc gagggcatcg tgatgagcga
     4681 aggcatcgtc atgagcgaag gcatcgtgat gagcgagggc atcgtgatga gcgagggcat
     4741 cgtcatgagc gaggacgcct cgctgggcga gccgtgaacg cgacgagtgc cgccggcgtc
     4801 gcggcgccgc cgcgcgccgc cggcaccggg tggctgccgc tgcaccagcg gctcatgccc
     4861 gactacaacc ggccggcggc cctctactgg tgggccgtcg tgctgctcgg gctggcggtg
     4921 ctgctgcact cgctggcgca cctggcgggc gtgccgccgg cggcgtgggt gcacgtcgcc
     4981 gccggcacgc tgctcgcgat gctggccggg ctcgtcccgg tgcgcatccc gcgctcgacc
     5041 aactccttca ccgccggcga gatcttcatc ttcctgctgc tgctgctcga cggcgcgcag
     5101 gccgccgcgc tcgcctccgc ctgcgaggcg ctggtcggct cgtggcgctc gtccaagcgc
     5161 tggaccagtc gcatcgcgag cccggcgatg gcggcgctgg ccatgttcac cgccggctcc
     5221 gcgctgcacg cggcgctgga cgcgctgcgc cgccacggca tggcggccga cggcgttctg
     5281 atcgccgccg cggtgtgctt cgcgatcgcg tacttcgtgc tgaacaccgt gatggtgacg
     5341 atggtgggcc gcctgaagcg caacgagccg ctgcggctgc gcgccttcgt cgacagcttc
     5401 ggctggctgg gcgtggccta tgcgggcaac gcctcggtgg ccaccttcct ctacctcagc
     5461 ttccgcagtt ccggcatcgc ggtggtcgtc gcggccgtgc ccatcatcgt catcctgctg
     5521 gtcaccggcc acttctactt ccgccgcgag gaggccgagg aggcggtgcg caaggcgcgc
     5581 gagcagcagg cggcgcagcg ccagatgctc gagaacgccc ggcaggccgg gatggcggag
     5641 atcgcgacca acgtgctgca caacgtcggc aacgtgctca ccagcgtgaa cgtgtcggcc
     5701 gagctgatct gcaaccgcgt gcgcgcctcg gaggcggccg gcctgccgcg cgccgtgcgg
     5761 ctgctcgacg agcacgcgca cgacctcggc gagttcctca cgcgcgacgc caaggggcgc
     5821 ctgctgcccg agtacctcag gcacctggcg caggcgctga tggaggagcg cggcggcctg
     5881 ctgcaggagc tcgagtcgct cacgctgagc gtcaagcaca tcaaggagat cgtggcgacg
     5941 cagcagtcgt acgccggcgc gtccagcgtg gtggaggcga tccaggtgcg cgacctgctg
     6001 gaggacgcgc tgcgcatcaa caccgtcggc gccggcgacc gcgcgcccgc ggtcgtgcgc
     6061 gagttcgccg acgtgccgca ggcgatgctc gacaagcacc gcgtcctgct gatcctcgtg
     6121 aacctgatcc gcaacgccac gcaggcgatg gccggcatgg tgcacgtccc gcacgtgctg
     6181 cgcctgtccc tcggcgtgac cgagaaggcg ctgctgatcg tggtggagga cagcggcgag
     6241 ggcatcgcgc cggagaacct cacgcgcatc ttcgcccacg gcttcaccac caagaaggac
     6301 ggccacggct tcggactgca cagctgcgcg cttgccgcgc gcgagatggg cggcgcgctc
     6361 accgcccaca gcgacggccc cggccgcggc gcgcgcttca ccctcgagct tcccttcaac
     6421 atccctcccg agaccaccgc atgaacgtcg cgaaccgacg catcctgctc gtcgacgaca
     6481 tgccgacgat ccacgaggac ttccgcaaga tcctcgcccc gccgacgacg gcgagcgccg
     6541 cgctgctcgc cgccaaggcg gcgctgttcg gcgacgacac cccggcgccg gccgtcaact
     6601 tcgagctcga ctcggccttc caggggcagg aggggctggc gaaggtggag gcgtcgctgc
     6661 aggcgggcct gccgtacgcc atggccttcg tcgacatgcg catgccgccc ggctgggacg
     6721 gcgccgagac cgtggcgcgc ctgtggcagg ccgatcccaa gctgcagatc gtcatctgca
     6781 cggcctactc cgactcgccg tgggaggagg tgctggagcg cctcgacgcg cgcgaccggc
     6841 tgctcatcct gaagaagccg ttcgacaaca tcgaggtgcg ccagctcgcc aacgcgctca
     6901 ccgcgaaatg ggcgctgacg cagcaggccg ccctgcacat cgaccgcctg gaggacgcgg
     6961 tgcaggagcg cacccgcaag atcagcgacg ccaacgaggc gctgcgcgcc gagatcgccg
     7021 agcgcaagca cctcgaaggc cagctcgtgc agtcggagaa gctcgcctcg atcgggcagc
     7081 tcgccgccgg agtggcgcac gagatcaaca atcccatcgg ctacatcttc tccaacttca
     7141 acacgctgga ttcgtacctc ggcgacctgt tcgaggtgct cggggcctac gaggcggccg
     7201 agcccagcat ggcgccgccg agcgtggcgg cgggcctggc gacgctgcgc gcgcagcgcc
     7261 agctcgactt cctgaaggag gacgtgcccg ggctgatgcg cgagtccaag gaaggcatcg
     7321 cgcgcgtgcg cgccatcgtg caggacctga aggacttctc gcgcgtcgac tccgcgcagg
     7381 agtggcagcg cgcgagcctg caccagggca tcgattcgac cctcaacgtc gtcgcgagcg
     7441 aggtccgcta caaggccgac gtggtgaagg agtacggaca ggtccccgag atcgagtgcc
     7501 tgccctcgca gatcaaccag gtggtgatga acctggtggt caacgcggcg cacgcgatgg
     7561 ggccggagcg cgggcgcatc accatccgca cgggcgccga gggcgaccag gcgtggttcg
     7621 aggtggcgga caccggctgc ggcatcccgc cggagaacct gtcgcgcatc ttcgatccgt
     7681 tcttcaccac caagccgatc ggcaagggca cggggctcgg cctgtcgctg tcgtacggca
     7741 tcgtgcagaa gcaccgcggc cgcatcgacg tgtgcagcga gcgggggcgc ggcacgacgt
     7801 tccgcgtggt gctgccggtg cgtcacgccg cggaggaggc ggccctgcca tgaacggcgt
     7861 gtcgatcatc gaggcggccg ccggccgccg catcctgctg gtggacgacg agcccaacgt
     7921 gctgagcgcg ctgcggcgtt cgctgcgcgg gcgcggcttc gacatcgcga ccgcgggcaa
     7981 cggcgagcaa gcgctcgccg agctgcgccg ggagcccgtc gacgcgatcg tctgcgacat
     8041 gcgcatgccg ggcatgagcg gcgcggaggt gctgaaggcc tcgatcgccc tcgcgcccga
     8101 cgccgttcgc gtgctgctca cgggctacgc cgacgtcgag tccaccgtgc gcgcggtcaa
     8161 cgagggcgag atcttccgct acctcgccaa gccctgggac gacgccttct tgctgcaggt
     8221 gctggacgac ggcctcgcgc gcaaggcgct ggagcgcgag cgcgacgcgc tgctcgcgct
     8281 caccgagcag cagaaccgcg agctgcgcac gttcaacgcc cgcctggagg cgcaggtggc
     8341 gcagcgcacg caggagctgc aggcgagcat ggccgagctg cgcaaggcca ccgagcggct
     8401 gaaggacgac ttcgcgggca cggtgcggct gctctcgggc ctcgtccagg gccgcgccgg
     8461 cctcaccgcg cgatgcccca gcgccgtggc gcggcacgtg cgcgccttcg cgccgcgcct
     8521 ggatctcgcc ggcgaggcgc tgcacgacct caccttcgct gcgctgctgc aggacctcgg
     8581 caaggtcgcg ctgcccgacg ccctcgtgcg gcagccgttc gaggcgctcg acaccccggc
     8641 ccggcaactc ctgctgaacc atccgcagat cggccgcatc tcgatggcgt cgctggcgtc
     8701 gctgcgcggc gcgggggaga tcctcggcag cgtgcaggag aacttcgacg gcagcggcgt
     8761 gcccggcaag gcgagcggcg aggacatccc gctcggcgcg cgcgcgctgc gcgtggcctc
     8821 cgacttcgag cagtacctcg ccggcgcgct gacgctggag ccgctctcgc cggagcaggc
     8881 gttccgccgg ctgcgccagt cgcgcggcac gcgctacgac ccgcgcgcgg tcgacgcctt
     8941 cctcgcctac atggagcagc ccgtgacggg cccggcgcgc aaggtgctca tgtcgagcgc
     9001 caacgtgcgc gtcggcatgc ggctcgcgca ggacctggtg accggcgcgg gctcgttgct
     9061 gctggcccgc ggctacgtgc tcgacgaggg cgtcatcgcg cagttgcgcc gcctggagga
     9121 gttctcgggc gagttcctct ggatcgcggt cagcagcgac gacggcgtgg aggtgcgcgt
     9181 gggcgaggcc gtggcgccgt gaccgccgcc gagtgcgggg atccggtacg agcgcccgtc
     9241 gtcctgcaac cgctgctgga cgcgctggac cacggtgccg acacgctggt ggtgacgtgc
     9301 gccgagctgc gcgtgcggta cgccaaccgc gccgcgcggc gcctgctggg cgaggcggtc
     9361 gcgccgggtg cgccgttcac gctgggtgcg cgggcgaccg tggcgggctt cgcccatctc
     9421 acgctcgccg acctgcaggg cgacgcgcgc gcgatcgagc tgcgcgtgtc gagcggcgac
     9481 tggctgggcg agcccgcgct gttccatgcg ctgcgcgacg tcacccagcg gcgcgccgcg
     9541 gagcagggcc tggtgctcgg acggcaggcg atggaggcga gcgccaacgg catcatgatc
     9601 gtcgacgcgg gcgacccgca gcagccgatc atctacgtga atccggcctt cgagcgcatc
     9661 acgggatacc ggcgcggcga agccatcggg cgcaacggcc gcttcctgca ggggcacgag
     9721 cgcgaccagc cgtcgctgcg cgcgctgcgc gaggcgctcg ccgccggcac cgacgtgagc
     9781 gtggtgctgc gcaactaccg caaggacggc acgcccttct ggaacgagct gtcggtgagc
     9841 ccggtgcgcg acgccgccgg ccgcgtcacc cacttcgtcg gcatccagaa cgacgtgacg
     9901 gagcggttgc gccaggaggc ggagctcgtg cggcgctcca cgcacgacga gctgaccgac
     9961 ctgcccaacc gctcggtgct gctcgagcgg ctgcgccagg cgctgcgcga gcgcggccgc
    10021 gcgcacgggg cgctcgccct gctgctcgtc gacctggacc ggttcaaggc gatcaacgac
    10081 gccctcggcc acgacgcggg agacgaggtg ctgttcgaga cggcggcgcg cctgcgcggg
    10141 tgcctggggg atcgcgacac gctggcgcgg ctgaacggcg accagttcgt ggcgctgctg
    10201 ccggcacgcg agggcgatgc cgcggtgaac gacgtggcgc aggcgatgct gcgcgccgtc
    10261 gccgagccct accaggtggc gggtcagacg gtgttctgca cctgcagcgt gggcatcgcc
    10321 ttcgccgggg acgcgcagga ggagcccgcg cagctgctgc agtgcgcgga cctggcggtg
    10381 catgccgcca agcgcggcgg tcgcaacgcc gcccacgcct acagcgccga catggtgcag
    10441 cgcgccagcg accgcatggc catgcgcaac gcgctgcacg acgcgatcca gcgcggcgag
    10501 ctgcacctgc actaccagcc gcagctcgac gtgcgctccc agcgcatctg cggcgtcgag
    10561 gcgctcgtgc gatggcgcca tccgacgctc ggcccgctgg caccggccga tttcattccc
    10621 ctcgcggagg agaccgggca gatcgtggcg ctcggcgagt gggtgatggc gcgcgcgtgc
    10681 gagcagcacc gacagtggct cgaccgtggc ctgatcgacg gcccggtggc ggtgaacgtg
    10741 tcggtcgtgc agttccggcg ggcggggttc gtcgaccggg tcgccgagat ccttgcgcgc
    10801 accggcctgc cgcccgggcg gctcgagctc gagctcaccg aatcggtcat gatggagcgc
    10861 ggcgaatgca gcttcgaggc gctgcaccgc ctgcgcgcgc tcggcgtgcg gttgtccatc
    10921 gacgacttcg gcaccggctt ctcgagcctc ggctacctga agcggctgcc gatcgacaag
    10981 atcaagatcg accgctcgtt cgtgcgcgac atcacctccg acggcgacga cgccgccatc
    11041 acgctcggca tcatcgcgat ggcgcaccac ctgcgcatgc gcgtcatcgc cgaaggcgtg
    11101 gaaacgccgg cccagctcgc ctacctgcgg cgccacctgt gcgacgaggc gcagggcttc
    11161 cacgtgtcgg cgcctttgcc gcccgcggcg ctggaggcct tcctcgcggc tttcgccccc
    11221 cctgccgtgg aaacggcagc cggcgacgac gccgcgccgc ccacgctgct gctggtcgac
    11281 gacgaaccca acatcctgag cgcgctcacg cgcacgctgc gccgcgacgg ctaccgcatc
    11341 ttcaccgccg ggggggccga ccaggcgttc caggtgctgg cgcagcagga ggtgcaggtc
    11401 atcctgtccg accagcgcat gccggccatg tgcgggacgg actttctgag cgaggtgaag
    11461 cggctgtatc cggacacggt gcgcatggtg ttgagcggct acaccgatct gcagtcggtg
    11521 accgaggcga tcaaccgcgg ggcgatctac cgctttctca ccaagccgtg ggaggacgag
    11581 ccgctgcgcg cccacatccg cgaggccttc gcccaccatc agcgcgagcg cctcggcgcg
    11641 gcgctcgctt gatggccgcc ggcgtcattc accgtgccgc gggcagtgtc gcgccgccgg
    11701 ccggcttccg tcgatggcgg gcgatggcac catcgcgccg cggagaacga cgatgaacaa
    11761 acctcggacc ctcggagccg ccggctgccg gccggatggc ggagcgccgc cgtgagccag
    11821 tggcccggcc ggctgcgtgt gctcggcatc gacgacgagg cggacgacct cgagctgatc
    11881 cgcctggcgc tccagggcgg cggcgtcgac gccgacgtga tccccgtgtg ggacgaggac
    11941 gggctgcgtc gggccctgga gcccgcaccc gacctggtcc tgtgcgacta cagcatgccc
    12001 ggcttcagcg ccgagcgcgc catggccgtc ctcgccgagc gcgcgccgca cctgccgctc
    12061 gtcgtggtga cgcgtgcgat cggcgaggag gcggtggtcg gcctgtttcg cgccggggcg
    12121 aaggactacg tcggcaagga caagctcggg ctgctgcccg gggtggtgcg gcgcgtgctg
    12181 tccgagcgct cgatggcgct cgagcgcgag cgggccgcgc aggcgctggc atccgccaac
    12241 acccggctgc ggctgctctc gtcgcacctg gtcgacgccc aggagcgcga gcgcacgcgc
    12301 atcgcgcgcg agctgcacga cgggctgggc cagctgctca ccggggtggt gatccagctg
    12361 caggccgccg ggaacagcca ggtgcccgag caggcggagc gctgccggcg ctgggccctg
    12421 gaactggccg agcagggcat ccgacaggtc aagacgatgt cgttccagct tcgcccgccg
    12481 cagttcgagc tgctcggatt cgcggcggcg gtgcaggcga cgctcgatcg ccagctcgaa
    12541 gccacctgcg tgcagggcgg cgtgcgtctg cgcggcgtcg acccgcacgt cgccggcgcg
    12601 gcgcaggtgg tggcgctgcg cgtgctgcag gaagcgttga ccaacgtgct gcgccatgcg
    12661 ggtgcgcggc gcgtcctggt gcgcgtgcgc ttcatggacg cgaaccgctt cgtgctcacg
    12721 ctgggtgacg acgggtgcgg cttcgacgtg aaggcggcgc tcggcgaggg tctcagcgaa
    12781 cgcaacctcg gcctgcacgg catgctcgag cgcgccgagc tgatgggcgg gcgcctgagg
    12841 ttccgttcct cccccgggcg cggcacggtg gtgcgcctgg tttccagcgc cgacgaggcg
    12901 gcgacgccgc gatggacatg agcggcgcgc aggagatccg cacggtgctc gcggatgacc
    12961 acgagctcgt tcgcgcgggc ttgacgctgc tgctcgagcg cgtgccgcgc gtgcgggtgg
    13021 tcggccaggc gagcagcggg gaggaactgc ttgcggtggc ccggcgcgaa cgacccgcgc
    13081 tggtggtcac ggacatctcg atgcccggca tgaacgggct tgcggccatc ggggagctgc
    13141 gtgcgctgcc cgatccgccg cgcatcctgg tcgtctcgat gcacgactcg cccgatttca
    13201 tccggcgcgc catgacgctt ggcgcgagcg gctacgtgat gaaggaaggc tccgcggtgg
    13261 agctcgagca ggcggtgcgc cgcgtgatgg gcggtcacac ctacttcagc ccgcaggtct
    13321 cgcaaaagat gatgcagcgg ccggacccgg ccccggaaga ggtgctgacg gagcgccagc
    13381 tggaaatcct ggtgctcatc gccagcggcc acgccagcaa ggaagccgcg ttcaagctcg
    13441 gcttgagccc gaagaccgtc gacgtgcacc gcgcgcggat catggagcgg ctcggcatcg
    13501 acgacgtcgc ggggctgacg ctgtactgcg tgcggcacgg gctggtggat ccgcgccgcg
    13561 ccgagtgacg atgcggcgcc ggccgctgcc ggacagggcg atgtccggca ggcgcgtgcg
    13621 cgcgaccgcc ggcagctgcg gccacgcgtc cgggctcgcc gggtctattt cttgtcggag
    13681 gacttcttct cggccggtgc cttggacgcc gatttcggca tcgacgtcgt cgggaccatg
    13741 atgtgggcgt aaggcgttcc cttccacatc acccacgggc cacccttggt ccaatccgtc
    13801 gggtacgcct cgagctgacc gacgtccgtc ggaaggatca tgatgtgcgg tccgctcacg
    13861 acccactggt tgtcggcggt cggcttcgtc gcgaatggat cggtgttgct ggcgcccttg
    13921 tcgcccttga gcatgtaggc cacgccgacc gacttcggct tcggcgggtc cttcttgttc
    13981 atccaggcgt cgccccaggc ctgccactcc ttgtcgaggc acatggagtc gccgacgaga
    14041 tcgagcatgc acatccatcc attggtgccg gcgcgcaatt ccttcatctt gccgtcggca
    14101 cccgtgccca tgaccgcggc gttgcggccg atgtccggcg gggcggcact cgtcgccttg
    14161 gcgatgacgg cggcgtcgga tctggccccg ccggccttgg ccatgtgctc gtggccggac
    14221 ttgggttggt ctttcgcttt ttcctgcgcc accgcggtga cggcagcgag catgagcacg
    14281 gcgcttatcg acgcgacgat ccttcgcatg atgcctcctg ttgccagata tggcagaaac
    14341 gaacgcattg tttggcagga ccaagcgtag acggtgcggt gagtttgtca aacgacggtc
    14401 ttgtcgtgac ttcgagcgga tggccggggc tccttgccga ccgcccgtca ggcgcggcgc
    14461 gcgcgcttct tcggcgcggg cggctcgacg gtgccgaaga tgcgctcgaa gttcacctcg
    14521 cccgcaccgg cggcgggcgc ctcgatgccg ctttccacgt ggtccaggtg ctgcagcatc
    14581 agccgctcgg cccggcgagc gtcgcccgcc tcgatggcct tcacgatgtc ggcgtgctcg
    14641 ttctcggggc aggcgctgct cgcctgggcg tcgtactggg cgatgatcag gccggtgagg
    14701 gccaccaggc ggcgcacgtt ctcggtgaag atccggttgc ccgtcgcttc ggccaggcgg
    14761 acgtggaagt caccggtgag ccgtgcgagc gccgcgcggt cgccctcgcc gcgcgcgcgc
    14821 gcctcgcttt ccaggtgcgc gcgcagcggg gcgatcggct gcccgccgca ccgctcggac
    14881 agctcggcgg cgatggcacg ctccagtgcg cgccgaacgg cgaacacgtg gcgcatgtcc
    14941 tccgtggtgg ggctcgcgac gaaggctccg cggttcgggt gcgactcgac gatcagctcc
    15001 tgacccagcc tcagcagcac ctcgcgcacg cgggtgcggc tcgcgcggaa cgcctgcgcc
    15061 aggcgctcct cgctcagctt ggtgccgggc agcagcctgc gctcgctgat ggcgctgtag
    15121 atgcgatcgt agatgtcctg gttgctcagc ttcgagttca cggccggaag tccgacgtgc
    15181 ggcggatcat ccgccgagat acaggcgctt cacgtgctcg ttggtcgtca agtccttcga
    15241 cggcccctcg agcgcgacgc ggcctgtctc cagcacgtaa gcgtacgacg atagcgcaag
    15301 cgccatccgg ctgttctgct cgacgagcac gaccgtgatg ccgtcgttgc ggttgatctc
    15361 gagcaggctg cggccgatga gccggatcac ctgcggcgcg atgccgaggg acggttcgtc
    15421 cagcagcagc agcttcggcc gcgccatcag cgcgcggccg atggccacca tctgctgctc
    15481 cccgcccgac aggctggagc cctgctgatg gaagcgctcc ttcagccgcg ggaagcgttc
    15541 gcacaccttg tccaggtcgg cggcgatgcc ggccttgtcg tgccgcgtgt aggcgcccat
    15601 gagcaggttg tccttgatgc tcatgtacgg gaagacatgc cgcccctcgg gcaccatcgc
    15661 cacgccctgg cgcaccagcg cgtgcgcggg ttcctgtcgc ccgggcgagt tgacgacgcg
    15721 gccctccagc cgcacctcgc ctgtggccac gcggcgcagg ccggtgatgg cgcgcagcgt
    15781 ggaggacttg cccgcgccgt tggcgccgac gagcgcgacg atcgccccgc gcggcacgac
    15841 gaggctcacc tgctccaggg cgcgcacctc gccgtagcgc agcgacacac ccgcgagctc
    15901 gaggtgcgca ggctcgcggg cgcgccgctc ggcagccgcc tcgcgcgcgg cgggcggagg
    15961 tcgcgcgtcc ttctccagca gttcggtcac agcccgagct cctcgtcttc ctgtccgagg
    16021 taggcctcga tcaccttcgg gtcgtcgcgg atctcggcgg gagtgccttc ggcgatcttg
    16081 cggccgaaat ggagcacatg gatgcgatcg gagatcgcca tcaccgcccg catgtcgtgc
    16141 tcgaccagca gcaccgacac gcccgaggcg gcgatcttgc gagtgatggc cactgcacgg
    16201 tcggtctcct cggggttgag cccggcatag ggctcgtcca gcagcagcac gcgcggccgc
    16261 gcggcgagtc ccatcgcgat gcccagcagt cgcagcaggc cctgcggcag gtgccgcgcc
    16321 gcgacgtcgg ccgccggcga cagttccatc agcgcgagga tcgcgcgcgc gctcgcggcc
    16381 agccggtcct cgtcggcgcg ggcgccccgc gtgccgagca cgacctgcag gtcgttcgcc
    16441 tcgcggtgca gctgatgggc gagcgacacg tgctccagcg cggtcaggtc ggggaagatg
    16501 gtcgtttcct ggaacgtgcg caccagcccg aggcgagcga tgcggtgcgg cggcaggccg
    16561 gtgatgtcct gcccgtgcag cagcacgctg ccgctggagg gcttcaggaa gcccgacagc
    16621 atcttgaaga acgtgctctt gcccgcgccg ttgggcccga tcaccgacac cacctgtccc
    16681 gcgggccccg tcagcaccag gtcgacatgc tcgttggcga cgaggccgcc gaagcgcttg
    16741 gtgataccgc gcgcctcgag gacggcttgc gccaggccgg cggtggcctc cagcggcacg
    16801 tctcgcggct tcatgccttc gcctccagcg cccgcgcccg gatcgccatc agcccgttgg
    16861 gcagcagcag gatcacgccg atcatcagca cgccgtagac cagcgtctgg tactgctgga
    16921 actggctcag cacctcgaac aggagggtca gcacgaacgc gccgatcatg gcgccgctca
    16981 cgaactcgag cccgccgagg aagcagtaca gcatgtagta gatgctgtgc tcgacggtga
    17041 agctctgcgg gtagacgctc tgcgtgaata cggcgaagta gctgccgccc aggccgccca
    17101 gcgcgcaagc gatggcgaag gcggtcacgc ggtagcgcca cacgtcggtg ccgaagctct
    17161 cggccagatc ctcgttcagg cgcatcgcgc ggaagatgcg gccgacgcgc gagtagtgaa
    17221 cgcgccaggt cacgagcagt gcgacgccga gcagcgcgat ggcgaggtag tacatcggca
    17281 gcggcgactc gccggcgaac ggctgcggca gtccggtgat gcccctggag ccctgcgtga
    17341 actcgccgcc gttgaggaag gcgagccgga tcgcctcggt gaggcagatg gtgagcatcg
    17401 agaaatagat gccgcgcagc ttcaggatga agctgccgat gatcgccccg agcgcggccg
    17461 aggcgagcgc cgacagcggc agcgcgagcc agaagctcat gccgtggcgc gacagcacga
    17521 tggccgtggt gtagccgccg atcagcgcga agccggcctg gcagatgttg atgcggccga
    17581 tggtgaaggt gagccacacg ccgaaggcca ccacggaaag gcccgcgcag ttcatcagta
    17641 ccgacagcag gtagcgcgaa gggccgatgg ccagcggcag cgccgcgagt gccgcgagga
    17701 agcacagggc gatcgcgaga tcgcgcttct tgagaggatt catgcggggt ccttttgcgc
    17761 cgggtcccga cgttgcgccg catgcggcgg catcaacccc acggcttgcc gaacagcccg
    17821 ttcggcttga agacgaggaa cacgatcatc gcgacgaaga tcagcaggta ggtgatgctg
    17881 ccgggcagca ggtcgtagcc gatcgcctcg gcgaagccga gcagcacgcc gccgacgatg
    17941 gcgcccgagg tcacgccggc gccgccgatc atgaccatga tgaacgcctt ggtggagatg
    18001 ttgacgccca cgccggagtt cacgccgtac accgtgacga gcaggccacc cgcgaggccg
    18061 gccagccccg cgccgatggc gaagccgagc gtggtggtgg ccttcacgtc cacgccctgc
    18121 aggcgcgccg cctccttgtc ctgcgcgacc gcgcgcagcg cccggcccgg ccgcgtgtac
    18181 tgcacgaagg cgagcagccc gaggatcgac agcgccgcca gggcggccac catggcgttg
    18241 ggatgggtga ggatggcgcc gccctccgcg acatcgccga agacgaaggc gccgtcgacc
    18301 accggcggca cgccgcgctg cttctcgccg aacaccgtga gggcgaggtt ctcgagcagc
    18361 agcgccgtgc cgaccgccat cagcatggag ttctcctcgc gcgtggcgct ggccagcacc
    18421 ggctggaaga agaagcggtt gaaggcgacg cccaccagcg ccagcgccgc cgcggcggcg
    18481 ggcagggcga cgtagaacgg caggccgtac tcgccgtaca ggtagtagac gacgaacgca
    18541 cccagcatgt acatctggcc gtgcgcgaaa ttcacgacgc gcatgatgct gaacaccatc
    18601 gtcaggccca gcgcgacgag ggcatagacg cagcccagca ccgcgccgtt gatgagcacc
    18661 tggatcatcg cgccgcccct gcctgcccgc agctcagttg cggatcgtcc ccacgaacag
    18721 cgtcttgaat tccttgtcct ggaactcgtt gatcaccagc ggcacgccca cctggctgcg
    18781 ccggccgaag tcggcctggc ctgcgaactc gagccgggtg gcgccgccct tgacgaaggg
    18841 gttgggcatc gagaaggtcg ccatctcctt cttgaacacc tcgacgtcgt cgagcgcctt
    18901 cggattcttc ttcagcaccg cgagaatcag ctccagcgcg tacaccttcg tgccggcctc
    18961 gtcgttccac tcgcccacgc gcttcttgta gaccgagacg aactccttca tgtagtcgga
    19021 ctggatctcg ggagtggacg ccccgcccac gctcatgaag ccgttggcct tggcgccggc
    19081 gacctgggcg aggatcttgg aatcctgcgc cgtctcggtg gagatcgtgc ccttgaagcc
    19141 caggtcgcgc gcggccttgt tgagcagcgg cgcgtcggcc ggcgccacgc ccgacagcac
    19201 gatcgcgtcg ggcttggtct tgatgacctt ggtcatcacc ggcatgaaat ccttggtgcc
    19261 cggctcgtag ctgtcgctgg cggcgaccac gttcagcccc agcttcttcg ccgcctcgac
    19321 accctccgcc tgctggttcc tggcgtcgga ttcgttgcgc gccaggaagg cgatcgactt
    19381 cacgcccttc ttggtcttca ggtactcgta catgatcgga ccgacttggt agctcgccac
    19441 catgcccagc acggagttgc cggccggcgc cttgtacagc tccttggcga aggcgtacgg
    19501 gaagttgagc gccttggcgc gctcgaccac gggcttcacc gccagcgccg tggtgtcgat
    19561 gttcgggccg atgatgtact tgatgccctg gctggtgagc ttctcggcgt tggtcaccga
    19621 gatcttgggg tcgttcttgt cgtcgagggc aacgatctcg atgcggtatc tcttgccgcc
    19681 gatgtccgcg ccgcccttgg cgttgaccat gtcggctgtg gtctcggcgc agtacttgtt
    19741 gaccaggccc caggaggccg cctcgcccga catcacgccg ttgacgccga tgcgcagcac
    19801 ctcctgcgcg cgagaaggcg cgccgaacag cgcgagcggc gtggcagcgg cgaggagggt
    19861 caggtgcaag cggatacggt ggttcatgag gcgtcctttt tcgagagggt ccatgtcggt
    19921 gcgcggcccg cgaccgcgcc gggtggcacg caagatccag accagtttgc atgcacttgt
    19981 tgcacgcaag ttgtgcttgc gattgtcgac aatctagact agcgtattgc gcacaaaggc
    20041 ggtatttacc cgcagccacg aggagaccac ggtgcgaatc caggtgatca atccgaacac
    20101 gacgcgctcg atgaccgagc gcatcggggc cgccgcacgc ttcgtggccg gccccggcgt
    20161 gacgatcgag gcggtgaatc cctcgcaggg ccccgcatcg atcgagggcc actacgacga
    20221 ggccctggcg tgcgtcggcc tgctgcacga agtgcgccaa ggcgtggtgg cgggcgtgga
    20281 cgcccacgtc atcgcctgct tcggcgaccc ggggctcgat gccgcgcgcg agatcgcgcg
    20341 cgggccggtg atcggcatcg ccgaggccgc catgcgctcg gcgtgctacc tggcgaccgg
    20401 cttctcggtg gtcacgacgc tcaagcgcac ccaggtgatc gccgagcacc tcgtcgacaa
    20461 atacggggtg cgaccatact gcaaacgcgt gcgcgcctgc gagattcccg tgctcgagct
    20521 ggaagatccc cagagcgatg cataccgcac catcctggcc gagtgccgcc gtgcgctcgc
    20581 cgaggacgac agcgccgcca tcgtgctcgg ctgcgccggc atggccgacc tgtgcgagcg
    20641 ccttcaggcc gagctcggcg tgccggtggt cgatggcgtc gcggcggcca ccgtcaccgc
    20701 gcaggccctc gtgcgcgcac gcctggccac cagcaagcgc ggcgactacg cgcggccgct
    20761 gcccaagccg tacgctggag cactcgccgc gttcgcgatc gagtgattca tgagctaccc
    20821 gagggacctg cgcggccacg gccgccaccc gccccacgcg cgctggcccg gcaacgcgcg
    20881 catcgcgctg cagttcgtcc tgaactacga ggaaggcggc gagaactcgg tgctgcacgg
    20941 cgacgccggc agcgagcag
//
LOCUS       DASRIE010000077         6468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2951720, whole genome shotgun sequence.
ACCESSION   DASRIE010000077 DASRIE010000000
VERSION     DASRIE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951720"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="VFB53_03640"
     CDS             <1..2569
                     /locus_tag="VFB53_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85858.1"
                     /translation="PLMSAGLVRAHRGAAGESLAMLYFTNSLGAAAGVLASGFVLIAW
                     IGLPGTLRTAGLLNLLIAAVVWMLARPARHRPIRPASDADGGAAPLLLTVAFFTGLAS
                     FVYEIAWIRMLSLVLGASTHAFELMLATFIFGLALGALAVRRYVDAAAEPARLLAWVQ
                     IAMGLAAAATLPVYNTTFSLMEALLKGLARSETGYLLFNLSGGAIAALVMLPATFCAG
                     MTLPLITGALLRRGAGEAAIGHVYAANTLGAIAGVLAAVHVGLPFLGLKGTLVAGAVI
                     DVALGLVLLNFLELRKALRTATVACGALLVAVAALVQLDAHKMTAGVFRHGDAGSSHE
                     ATILFNKDGKTATVHLVKYRDATSLRTNGKSDGSINMDADGERGTDEITMVLTAALPL
                     ALKPDTKSAAVIGIGTGLTTHTLLQSLDIERVETVEIEPAMAEAARGFMPRNSGAFAD
                     PRGAILIDDAKSYFSTRNRRYDLVISEPSNPWVSGVSSLFTREFYRRIRKHLNPGGLL
                     VQWIQLYEMDAALVATVIRALGEEFSHYAVFAPSDTDLLIIAGDAPIPLPPQAGVFEH
                     PGLAKELWTVHMLTAGDLDARYLGGRATLEALFASYGMPANSDYAPVLDRNAARHRFM
                     EKSASDVVALLNAELPLLELLEPGRSRRAINPLFKGAYAFERVENTRLAWYGRDFLLR
                     SRPPVPDGVPRDLQKDLELVKLRLIECREPRDLDAWLHGALRVAKALNPYLAADDVGA
                     VWARITRSPCHAGLHEFQRRWLALFQAVGARDAARMAELSSQLLATQDELGSDARDYL
                     LLAALAGYVASGDKAKALATWNSHSGKVRVSRPAFRLLRCHADPASCAAAFRSYAER"
     gene            complement(2554..3066)
                     /locus_tag="VFB53_03645"
     CDS             complement(2554..3066)
                     /locus_tag="VFB53_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85859.1"
                     /translation="MTTYHHGDLRAAVLSAAAKMIESEGLPGLSVREAARRAGVSHAA
                     PYRHFQRREALLAALAADGFVELEKVLEGKAGRSLGEAYVRFALERPQRFRLMFASRR
                     PEARAGYEKLKARLAGLGSEVEVAAAAAWSLVHGLALLAIDGHLASDPDFLKRAIGAV
                     RISIGAQRSA"
     gene            3282..3893
                     /locus_tag="VFB53_03650"
     CDS             3282..3893
                     /locus_tag="VFB53_03650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85860.1"
                     /translation="MFLALLRRGLLPDAGSLLDAGCGQGVLLSLLLAAREQYTAGRWP
                     RDWPPPPRRLALSGIDAHAGRVAVARHALGANAQVEHGDLREHVFARPYSAIVLIDVL
                     LYLDAGEGERVIRNAAAALQPDGLLLLREPDAGAEAGFALRLTRCSSWFDAAMRGRFG
                     QRLHFRSAAQWRAELARQGLAIEAQPMSQGTPFANVLFVAKKN"
     gene            complement(3890..5218)
                     /gene="der"
                     /locus_tag="VFB53_03655"
     CDS             complement(3890..5218)
                     /gene="der"
                     /locus_tag="VFB53_03655"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZN85861.1"
                     /translation="MTPVIALVGRPNVGKSTLFNRLTRSRDAIVHDVPGVTRDRHYGE
                     GRLGARPFIAIDTGGLEPGAPDGIFVHMARQAEQAIVESDAVVFMVDGRSGLTATDRE
                     IAARLRKIKTPRYLAVNKTEGMQSDIAVAEFHELGLGAPAAISAAHGEGVRELMDLVL
                     QGFPEDVEPAEEERGHPRVAVVGRPNVGKSTLVNALVGEERVITFDQPGTTRDPIEVP
                     FERGGRRYTLIDTAGLRRRGKTGEAVEYFSIVKALQAIEAANVAILLLDGATGITEQD
                     AHVAGYVLERGRAVVLAVNKWDAADKEARARMKQELAWKLGFLGFADTHFVSAREGKG
                     LAPLMRSVDAAYAAAMARLATPKLTRALIAAIERQSPPKVGRFRPKLRYAHQGGVNPP
                     RIIIHGNSLDHVPDSYKRYLEGFFRKKFSLTGTPMSIEFRTGRNPYANKR"
     gene            complement(5215..6342)
                     /gene="bamB"
                     /locus_tag="VFB53_03660"
     CDS             complement(5215..6342)
                     /gene="bamB"
                     /locus_tag="VFB53_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HZN85862.1"
                     /translation="MKRAGLAAVCFLAACSNTPGPQPAELGRIEKPQDVRVLWSSQVG
                     AAERFTFTPALVGDSVYVAARDGTVTGMDAAKGRERWRASLELQLSGGVGADPSTVAV
                     ASEEGVVVALDPATGKERWRARVSSEVLAQPTIGRGLVLVRTIDNKVFAFGTADGKRL
                     WVYQRAPTTLVVRMPAGVTIAGELAFVGFPGGKLAALTLANGAVRWEGTVSVPKGTTE
                     LERIADVMGDPVVQGREVCAAAYRGRVACFEAASGRPLWAREVGALSPVALDARYAYV
                     ADERGAVHALDRSNGQSVWKQDRLAYRGLSAPLPGGQAVAIGDFEGYVHFLARDSGAL
                     LARHEIGGGAIRATPVVLPSALPAAGLLVQTEGGVLFALAL"
BASE COUNT          904 a         2346 c         2230 g          988 t
ORIGIN      
        1 cccgctgatg agcgccgggc tggtgcgcgc ccatcgcggc gccgcgggcg agtcgctggc
       61 gatgctctac ttcaccaaca gcctgggagc ggcggccggc gtcctcgcca gcggcttcgt
      121 cctgatcgcc tggatcgggc tccccggcac gctgcgcacc gcgggcctgc tgaatctcct
      181 gatcgcggcc gtggtctgga tgctggcgcg cccggcgcgg caccgcccga tccggccggc
      241 gtcggatgcc gacggcggcg cggctccgct gctgctgacg gtggcttttt tcaccggcct
      301 cgcgtccttt gtctacgaga tcgcctggat ccgcatgctg tcgctggtgc ttggcgcctc
      361 gacgcatgcg ttcgagctga tgctcgccac cttcatcttc ggcctggcgc tgggcgcgct
      421 ggcggtgcgc cggtacgtcg acgccgcggc cgagccggcg cggctgctcg cctgggtgca
      481 gatcgcgatg gggctggccg ccgcggcgac gctgccggtc tacaacacca ccttctcgct
      541 catggaggcc ctgctgaaag gcctggcgcg cagcgaaacc ggatacctgc tgttcaacct
      601 ttccggcggg gcgatcgccg cgctggtcat gctgccggcc acgttctgcg ccgggatgac
      661 gctgccgctc atcaccggcg cgctgctgcg ccgcggcgcc ggcgaggcgg cgatcgggca
      721 tgtctacgcg gccaatacgc tcggcgcgat cgccggcgtg ctcgctgcgg tgcacgtcgg
      781 cctgccgttc ctcgggctga agggaacgct ggtggcgggc gcggtgatcg atgtcgcgct
      841 cggcctggtg ctgctgaatt ttctggagct gcgcaaggcg ctgcgcaccg ccacggtggc
      901 gtgcggcgcc ctgctggtcg ccgtcgcggc cctggttcag ctcgacgcgc acaagatgac
      961 cgccggggtg ttccgccacg gcgacgccgg ctcgtcgcac gaagccacca tcctgttcaa
     1021 caaggacggc aagaccgcga ccgtccacct ggtgaagtac cgcgacgcga ccagcctgcg
     1081 caccaacggc aagtccgacg gctcgatcaa catggacgcg gacggcgagc gcggcaccga
     1141 cgagatcacg atggtgctga ccgcggcgct gccgctcgcg ctcaagccgg acacgaaaag
     1201 cgccgcggtg atcggcatcg gcaccggcct caccacccac acgctgctgc agagcctcga
     1261 catcgagcgc gtcgagaccg tcgagatcga gccggcgatg gccgaggccg cgcgcggctt
     1321 catgccgcgc aacagcggcg ccttcgccga tccgcgcggt gcgatcctga tcgacgacgc
     1381 gaagagctac ttctcgaccc gtaaccgccg ctacgacctg gtgatctcgg agccttccaa
     1441 cccgtgggtg agcggggtat cgagcctgtt cacgcgcgag ttctaccgcc gcatccgcaa
     1501 gcacctgaac ccgggcgggc tgctggtgca gtggatccag ctctacgaga tggacgcggc
     1561 gctcgtcgcc acggtgatcc gggcgctggg cgaggagttc tcgcattacg ccgtgttcgc
     1621 gcccagcgat accgacctcc tgatcatcgc gggcgacgcg ccgataccgc tgccgccgca
     1681 ggcgggcgtg ttcgagcacc ccggcctggc gaaggagctg tggaccgtgc acatgctgac
     1741 cgccggcgac ctcgacgcgc gctacctcgg cggccgcgcc acgctcgagg cgctgttcgc
     1801 gagctacggc atgccggcca actcggacta cgcgccggtg ctcgaccgca acgcggcgcg
     1861 ccaccgcttc atggagaaga gcgcgtccga cgtggtcgcg ctgctgaacg cggagctgcc
     1921 gctgctcgag ctgctcgagc ccggccgcag ccgccgcgcg atcaacccgc tgttcaaggg
     1981 cgcctacgcc ttcgagcgcg tggaaaacac gcggctcgcc tggtacggcc gcgacttcct
     2041 gctgcgctcg cggccgccgg tcccggacgg ggtgccgcgc gacctgcaga aggacctcga
     2101 gctggtgaag ctgcgcctga tcgagtgccg cgagccgcgc gatctcgacg cctggctgca
     2161 cggcgcgctg cgcgtggcga aagcgctcaa tccgtacctc gcagccgacg acgtcggtgc
     2221 cgtgtgggcg cgcatcacca ggagtccctg ccatgcgggc ctgcatgaat tccagcgccg
     2281 ctggctcgcc ctgttccagg cggtcggcgc gcgcgatgcc gcacgcatgg cggagctgtc
     2341 ttcccagctt ctcgccacgc aggacgagct cggctcggat gcgcgcgact acctgctgct
     2401 cgccgcgctc gctggctacg tggcgagcgg cgacaaggcg aaagcgctcg cgacctggaa
     2461 ctcgcacagc ggcaaagtgc gcgtctcgcg ccccgcgttc cggctcctgc gctgccacgc
     2521 cgacccggcg agctgcgcgg ccgcgttccg ctcctacgcc gagcgctgag cgccgatcga
     2581 gatgcgcacc gcgccgatcg cccgcttcag gaaatccggg tcgcttgcca gatggccgtc
     2641 gatggcgagc agcgcgagcc cgtgcaccag cgaccacgcg gccgcggctg cgacctcgac
     2701 ttcgcttccc aagcctgcca ggcgggcttt cagcttctcg tagccggcgc gcgcctcggg
     2761 ccgacgtgac gcgaacatga ggcggaagcg ctgcggacgc tcgagtgcaa agcgcacgta
     2821 cgcttcccca agcgagcgcc cggccttgcc ctcgagcacc ttttccagct cgacaaatcc
     2881 gtcggcggcg agcgccgcca gcagcgcctc gcgccgctgg aaatgccggt acggcgccgc
     2941 gtgggatacc cccgcgcgcc gtgccgcctc ccgcacgctg agaccgggaa gtccctcact
     3001 ttcgatcatc ttggcagcgg cggataggac cgccgcccgc aggtcgccat ggtggtatgt
     3061 tgtcattgac tacattccgt taaccatcat gttgtcattg tctacttagc gcgcacgtat
     3121 ggcaaccccg tcgttataat cgcggggcgc ctctggaccc cgcttctcac tccgcttctc
     3181 acccatttgt caggcagctg ctcgacgccg cgagccagcc gtatgcggcg gccggctggt
     3241 acgcgcggca ttttgcaagc ggcaagctgc gcttcgaccc ggtgtttctc gccctgctgc
     3301 gccgcgggct ccttccggac gcgggcagcc tgctcgatgc cggctgcgga cagggcgtgc
     3361 tgctttcgct ccttctcgcg gcgagagagc agtacacggc aggccgctgg ccgcgggact
     3421 ggcccccgcc gccgcggcgc ctcgcgctga gcgggatcga tgcccacgcc ggccgcgtgg
     3481 cggtcgcgcg gcacgcgctt ggcgcgaacg cacaggtgga acacggcgat ctgcgcgagc
     3541 atgtcttcgc ccgcccctac tcggcgatcg tgctgatcga cgtgctcctc tacctcgacg
     3601 ctggagaggg cgaacgcgtg atccgcaacg ccgcggcggc gctccagccg gacgggctgc
     3661 tgctcctgcg cgagcccgat gccggagcgg aagcgggctt cgcgctgcgg ctcacccgat
     3721 gcagctcgtg gttcgatgcg gcgatgcgag gcaggttcgg gcagcggttg catttccgca
     3781 gcgcggccca gtggagggcg gaactcgcgc ggcaaggact cgccatcgag gcgcagccga
     3841 tgagccaggg cacgccgttc gccaacgttc tgttcgtggc gaaaaaaaat tagcgcttgt
     3901 tggcgtacgg attgcgcccg gtgcggaact cgatgctcat cggcgtaccc gtcagggaaa
     3961 atttctttct aaaaaaacct tccagatagc gcttgtagga atcgggcacg tggtcgaggg
     4021 agttgccgtg gatgatgatg cgcggcgggt tgacgccgcc ctggtgcgcg tagcgcagct
     4081 tggggcggaa gcggccgacc ttgggcgggg actggcgctc gatcgcggcg atcagagcgc
     4141 gcgtgagctt cggcgtcgcg agccgggcca tggcggcggc ataggcggcg tcgaccgaac
     4201 gcatcagcgg cgcgagcccc ttgccctccc gcgccgacac gaaatgcgtg tcggcgaagc
     4261 cgaggaagcc gagcttccag gcgagctcct gcttcatccg cgcgcgcgcc tccttgtccg
     4321 ccgcgtccca cttgttcacc gccagcacca ccgcgcggcc gcgctcgagc acgtagccgg
     4381 cgacgtgggc atcctgctcg gtgatcccgg tcgcgccgtc gagaagcagg atcgcgacgt
     4441 tggcggcttc gatcgcctgc agcgccttga cgatcgagaa gtattccacg gcttcgcccg
     4501 tcttcccgcg ccggcgcagg ccggcggtat cgatcagcgt gtagcgccgg ccgccgcgct
     4561 cgaacggcac ctcgatcggg tcgcgcgtgg tgccgggctg gtcgaaggta atgacgcgct
     4621 cctcgccgac gagcgcgttg accagcgtcg acttgccgac gttcgggcgc ccgaccaccg
     4681 cgacgcgggg atggccgcgc tcttcctccg ccggctccac gtcttcgggg aatccttgca
     4741 ataccaaatc catcagctcg cgcacgccct cgccatgcgc ggcggagatc gccgccggtg
     4801 cgcccagccc gagctcgtgg aactcggcga cggcgatgtc cgactgcatg ccctcggtct
     4861 tgttcaccgc gaggtaacgc ggcgtcttga tttttcgcag gcgcgcggcg atttcgcggt
     4921 cggtcgccgt cagaccgctg cggccgtcga ccatgaacac caccgcatcc gactcgacga
     4981 tcgcctgctc ggcctggcgc gccatgtgca cgaagatgcc gtcgggcgcg cccggctcca
     5041 ggccgccggt atcgatggcg atgaacggcc gcgcgccgag gcggccttcg ccgtagtgcc
     5101 ggtcgcgcgt caccccgggt acgtcgtgca cgatggcgtc gcggctgcgc gtcaggcggt
     5161 tgaacagcgt cgacttgccg acgttaggcc gccccaccaa ggcgatgacc ggggtcacag
     5221 cgcgagcgca aacaggacgc cgccttcggt ctgcaccagg agccccgccg cgggcaacgc
     5281 cgaggggaga accaccggcg tcgcacggat cgcgccgccg ccgatttcgt ggcgcgcgag
     5341 gagcgcaccc gagtcgcgcg cgagaaagtg cacatagccc tcgaagtcgc cgatcgcgac
     5401 cgcctgtccg ccggggagcg gcgccgacag tccgcggtac gcgaggcggt cctgcttcca
     5461 caccgactgc ccgttgctgc ggtcgagcgc gtgcaccgcg ccgcgctcgt cggcaacgta
     5521 ggcatagcgc gcatccagcg cgaccgggct gagcgcgccg acctcgcgcg cccagagcgg
     5581 ccggccgctg gcggcctcga agcacgccac gcgcccacgg taggcggcgg cgcacacttc
     5641 gcgcccctgc accaccgggt cgcccatgac gtcggcgatg cgctcgagct cggtggtgcc
     5701 cttcgggacg ctgaccgtcc cctcccagcg caccgcgcca ttggccaggg tcagtgcggc
     5761 gagcttgccg ccggggaagc cgacgaacgc gagctcgccc gcgatggtca cgcctgccgg
     5821 catgcgcacg acgagggtgg tcggcgcgcg ctggtagacc cagaggcgct tgccgtcggc
     5881 ggtaccgaag gcgaatacct tgttgtcgat ggtgcgcacc agcaccagcc ccctgccgat
     5941 cgtgggctgt gccagcacct cgctcgagac gcgcgcgcgc cagcgctcct tgccggtcgc
     6001 ggggtccagc gccaccacca ccccctcctc gctcgcaacg gcgacggtgc tcgggtcggc
     6061 gccaacgccg ccggacagtt gcagttcgag cgaggcgcgc cagcgctccc tgcccttggc
     6121 ggcgtccatg ccggtcacgg tgccgtcgcg ggcggcgacg tacaccgagt cgccgaccag
     6181 cgccggagtg aaagtgaacc gctcggcggc gccgacctgc gaggaccaca gcacgcgcac
     6241 gtcctgaggc ttttcgatcc gccccagctc ggcgggctgg ggccccgggg tattggaaca
     6301 ggcggcgagg aaacacaccg ccgcaagacc cgcgcgcttc acctcatccc ccgagggcgt
     6361 cgaggcgcat gcgcacgctg tcgcggaacg ggccctggtc cttgcccgcc ttctccagcg
     6421 cgagctggta ggcggcgcga gcctcggccg gctggttttt cgccacca
//
LOCUS       DASRIE010000078         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3743622, whole genome shotgun sequence.
ACCESSION   DASRIE010000078 DASRIE010000000
VERSION     DASRIE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743622"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFB53_03665"
     CDS             complement(<1..1127)
                     /locus_tag="VFB53_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN85863.1"
                     /translation="MSAHPSPPTGERRRTERRAKQRPEPPEESWFGAIGVDTQISSGF
                     EESRYEGGLAAAAEATPDSRFLSRQARRLVRSGQTAFDRIYRVFLGARAALGVALILT
                     VVVAGLFGGRATWPVAIVSVLYATHALSIWLVPGLKHNVQPHELARMGGKQWLGTIGA
                     DIVAFTTLHLLSPSSSFNYVALLVLPVLMAGVLTPRMQALATSSFVALSLLGSAWLAV
                     LRGGDATTLMTQAGLAGSGLFVITVLAGELAGRLAREELTAKGSLELARQQAQLNRLV
                     IEEMQDGVLVVDRRGRVRAANPAARRLLAPSGMCRPPPFQLRGVEAWSELVKTVDRAF
                     ADASWPESGRDVVVQFERSETRTLRVRIRFTRRHDEQASEE"
     gene            complement(1198..1440)
                     /locus_tag="VFB53_03670"
     CDS             complement(1198..1440)
                     /locus_tag="VFB53_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HZN85864.1"
                     /translation="MKYLLIVLVVLAVIWLARSARRLPGDAARRPPAPGADRKTMLAC
                     AQCGLHLPPDEALPGRGGVFCSEAHRAEYERAHPAP"
     gene            complement(1451..2266)
                     /gene="ccsA"
                     /locus_tag="VFB53_03675"
     CDS             complement(1451..2266)
                     /gene="ccsA"
                     /locus_tag="VFB53_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZN85865.1"
                     /translation="MILSAGSSSTWLLPGLLALAAYVLAALLPQRSTTAVRIALVLGW
                     LAHAGSILVETSGLGTSDPGARFGFAPALSATLWLVIAVYLVESRSLPLPGVRRTLCG
                     LGLLAVALALLFPGEARPNMLSRWEPLHVVTAIAAYGLFGAAVLHAALLNRADRQMRA
                     MPRHPEASGSFGLPLLRLERLTFRFVVAGFALLTLTLLVGWWVAPWQWSHKTVFSLLA
                     WLVFAGLLAGRQVFGWRGSLATRWLYAGASLLLLAYVGSRFVFEVLLHRPPAA"
     gene            2335..3696
                     /gene="ffh"
                     /locus_tag="VFB53_03680"
     CDS             2335..3696
                     /gene="ffh"
                     /locus_tag="VFB53_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZN85866.1"
                     /translation="MASTLTDRLSRLVKTMRGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKEKALGQEVVGSLSPGQALVGIVHKEIAATMGEGVADINLATQPPAV
                     ILMAGLQGAGKTTTTAKLAKHLIEKRRKKVLTVSADVYRPAAIEQLKTVTKQAGAEWF
                     PSHASDKPVDIAMAALEHARRHYADVLLVDTAGRLAIDEALMKEIADLHAALKPVETL
                     FVVDAMQGQDAINTAKAFREALPLTGIVLTKMDGDSRGGAALSVRQVTGAPIKFAGVS
                     EKIDGLEVFDAERHAGRILGMGDIVALVEEVTKGVDLAAAQKLADKVKAGDAFDLNDF
                     LSQLSQMKKMGGLSGLMDKLPSQLTAKAGAVDVDKAERDVRRMEGIICSMTPLERRKP
                     ELIKATRKRRIAAGAGVQVQDVNRLLNQFEQMRDMMKKMKGGGMMKMMKRMGGMKGFP
                     GPR"
BASE COUNT          625 a         1336 c         1379 g          432 t
ORIGIN      
        1 aactcctcgc tcgcctgctc gtcgtgccgg cgggtgaagc ggatgcgcac gcgcagcgtg
       61 cgcgtctcgc tgcgctcgaa ctgcaccacc acgtcgcggc ccgactcggg ccaggaggcg
      121 tcggcgaacg cgcggtccac cgtcttcacg agctcggacc acgcctcgac gccgcgcagc
      181 tggaacggcg gcggccggca catgccgctc ggcgcgagca gccggcgcgc ggccgggttg
      241 gcggcgcgca cccggccgcg gcggtcgacg acgaggacgc cgtcctgcat ctcctcgatg
      301 acgaggcggt tgagctgcgc ctgctggcgc gccagctcga ggctgccctt ggcagtgagc
      361 tcctcgcgcg cgagccggcc ggccagttcg ccggcgagca cggtgatgac gaacaggccg
      421 ctgccggcca ggcccgcctg cgtcatgagg gtggtggcgt cgccgccgcg cagcaccgcg
      481 agccaggcgc tgccgagcag cgagagggcg acgaacgacg acgtggccag cgcctgcatg
      541 cgcggcgtca gcacgccggc catcagcacc ggcagcacga gcagcgcaac atagttgaag
      601 ctcgacgagg gcgacagcag gtgcagcgtg gtgaacgcca cgatgtcggc gccgatcgtg
      661 ccgagccact gcttgccgcc catgcgcgcg agctcgtgcg gctgcacgtt gtgcttcagg
      721 ccggggacca gccagatgct gagcgcgtgc gtcgcataga gcacgctcac gatcgccacc
      781 ggccaggtgg cgcgcccgcc gaacagcccc gcgacgacca cggtcaggat cagcgccacg
      841 cccagcgccg cgcgcgcccc gaggaagacc cggtagatgc ggtcgaacgc cgtctgcccg
      901 gagcgcacga gccgccgcgc ctggcgcgac aggaagcgcg agtccggcgt ggcctcggcc
      961 gcggcggcca ggccgccttc gtagcgcgat tcctcgaagc cgctggagat ctgcgtgtcg
     1021 acgccgatcg cgccgaacca ggactcctcc ggcggctcgg ggcgctgctt ggcgcgccgt
     1081 tcggtgcgcc ggcgctcgcc ggtcggcggg ctggggtgcg cgctcattgg cgcaccctac
     1141 acacttcgcg cgatccgtcc gggaggcaag ccattcgccc ccagggcggc cgggggctca
     1201 tggcgcggga tgcgcgcgct cgtactcggc gcggtgggcc tcgctgcaga acacgccgcc
     1261 gcgcccgggc agggcctcgt ccggcggcag gtgcaagccg cattgcgcgc aggccagcat
     1321 ggtcttgcgg tcggcgccgg gggcgggcgg ccggcgcgcc gcatcgcccg gcaggcgcct
     1381 tgcgctgcgg gcgagccaga tcaccgccag caccaccagg acgatcagca ggtacttcat
     1441 gccgcccgct tcaggccgcc ggcggacggt gcagcagcac ctcgaacacg aagcgcgagc
     1501 cgacgtacgc gagcagcagc aggctcgctc ccgcatagag ccagcgcgtg gcgagcgagc
     1561 cgcgccagcc gaacacctgc cggcctgcga gcaggccggc gaacaccagc cacgccagca
     1621 gcgagaacac cgtcttgtgg ctccattgcc agggcgcgac ccaccagccg accagcagcg
     1681 tcaacgtgag cagcgcgaag cccgccacga cgaagcggaa ggtgaggcgc tccagccgca
     1741 gcagcggcag gccgaagctg cccgacgcct cgggatggcg cggcatggcg cgcatctggc
     1801 ggtcggcgcg gttgagcagc gccgcgtgca gcacggccgc gccgaacagg ccgtacgcgg
     1861 cgatggcggt gacgacgtgc agcggctccc agcgcgagag catgttgggc cgtgcctcgc
     1921 cggggaacag cagcgcgagc gccaccgcca gcaggccgag cccgcacagc gtgcggcgca
     1981 cgcccggcag cggcagcgag cggctctcca cgaggtacac cgcgatgacg agccacaggg
     2041 tggcggacag cgcgggcgcg aagccgaagc gcgcgccggg gtcgctggtg ccgaggcccg
     2101 aggtctccac caggatcgat cccgcgtgcg cgagccagcc gagcaccagg gcgatgcgca
     2161 ccgccgtcgt cgagcgctgc ggcagcagcg cggccaacac gtacgccgca agcgccagca
     2221 ggccgggcag cagccaggtg gaggaagacc ccgcagataa aatcatccgc gcagtgtact
     2281 ttcgcgctct cgttgcgcag catcccggcc gcgctcccac gtgccgcaaa ccacatggct
     2341 tccaccctca ccgaccggct gagccgtctc gtcaagacga tgcgcggcca ggcccgcatc
     2401 accgaatcca acgtgcagga catgctgcgc gaggtgcgca tggcgctgct cgaggccgac
     2461 gtggcgctgc ccgtggtgcg ggacttcatc gcgcgggtga aggagaaggc gctcggccag
     2521 gaggtggtcg gctcgctctc gccgggccag gcgctggtcg gcatcgtgca caaggagatc
     2581 gccgccacca tgggcgaggg cgtcgcggac atcaacctcg cgacgcagcc gcccgcggtc
     2641 atcctcatgg ccggcctgca gggcgcgggc aagacgacca ccaccgccaa gctcgccaag
     2701 catctcatcg agaagcgcag gaagaaggtg ctcacggtct cggccgacgt gtaccggccg
     2761 gccgccatcg agcagctgaa gacggtgacg aagcaggccg gcgccgagtg gttcccctcg
     2821 cacgccagcg acaagccggt cgacatcgcc atggccgcgc tggagcacgc gcggcgccat
     2881 tacgccgacg tgctgctcgt cgacaccgcc ggccgcctcg ccatcgacga ggcgctgatg
     2941 aaggagatcg ccgacctgca tgcggcgctc aagccggtgg agacgctctt cgtggtggac
     3001 gccatgcagg gccaggacgc gatcaacacc gccaaggcct tccgcgaggc gctgccgctc
     3061 accggcatcg tgctcaccaa gatggacggc gattcgcgcg ggggggcggc gctgtcggtg
     3121 cggcaggtga ccggcgcgcc catcaagttc gcgggcgtca gcgagaagat cgacgggctc
     3181 gaggtgttcg acgccgagcg ccacgcgggg cgcatcctcg gcatgggcga catcgtcgcg
     3241 ctcgtcgagg aagtgacgaa gggcgtcgac ctggcggccg cgcagaagct cgccgacaag
     3301 gtgaaggcgg gcgacgcctt cgacctgaat gacttcctgt cacagctttc acaaatgaag
     3361 aagatgggcg gcctgtccgg gctcatggac aagctgccca gccagctcac cgcgaaggcc
     3421 ggcgcggtcg acgtggacaa ggcggagcgc gacgtgcgcc gcatggaagg catcatctgc
     3481 tcgatgacgc cgctggagcg gcgcaagccc gagctcatca aggcgacccg caagcgccgc
     3541 atcgccgcag gcgcgggcgt gcaggtgcag gacgtgaacc gcctgctcaa ccagttcgag
     3601 cagatgcgcg acatgatgaa gaagatgaag ggcggcggga tgatgaagat gatgaagcgc
     3661 atgggcggga tgaaaggctt cccggggccg cgctaggcgt tgcgcacgcc cgagcgggcg
     3721 cgcacgcatc ggcctttccc cccgcatgcg gggagaggca gacgagccgg ct
//
LOCUS       DASRIE010000079         7549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_607821, whole genome shotgun sequence.
ACCESSION   DASRIE010000079 DASRIE010000000
VERSION     DASRIE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607821"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..2383
                     /locus_tag="VFB53_03685"
     CDS             392..2383
                     /locus_tag="VFB53_03685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZN85867.1"
                     /translation="MQVLRVALDVPLATLFDYSRPDSLEVAAGDRVVVPFGPRSRVGV
                     VIEKGEGSAIAAEKLKPVSRVLADTPRLPSQWLELMRFLATYYQRPLGETVIGALPPR
                     LRSLKPLPKAVREAAAPQPASPRFVGSHTPTADQAQAIERIGGALGRFEPFLLHGITG
                     SGKTEVYLRLIARVLEQGNQALVLVPEISLTPQLESRFRHAFPQAHIVMLHSALQDVP
                     RTAAWLAAARGEAGIVLGTRLATLTPLPRLALVVVDEEHDTSFKQQEGLRYSGRDAAV
                     MRAKFAACPVVLGTATPSLETWLNALVGRYARVSLPERAVPGARLPIVRTLDLRREPA
                     LHGFAPALIAAIGARLERREQSLVFINRRGYAPVLACHACGWTSGCERCTARMVLHKT
                     DRRLRCHHCGAESAIPRACPTCGNVDLKAMGRGTQRVEETLEARFPQARVVRIDRDSA
                     RRRSDLARTLEGIGRGEADIMVGTQLLAKGHDFPLLTLVGVLDADGALLSTDYRAAER
                     LHATLAQVAGRAGRREQPGEVLVQTSYPRHPLYAALASHDFAAFAAAQLAEREAAGFP
                     PYVHEAALRAEADKLALAMQFLDDAARLVPVPDEVRVYDPVPHVITRRADMERAQLLV
                     QSASRPALQGFLAAWSERLFATAPRNVRWHLDVDPIEFD"
     gene            2405..4021
                     /gene="argS"
                     /locus_tag="VFB53_03690"
     CDS             2405..4021
                     /gene="argS"
                     /locus_tag="VFB53_03690"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZN85868.1"
                     /translation="MDIKTHLAALFGQALAKIAPEAADAEVLIERPRDASHGDFACNL
                     ALQLAKRLKRSPRQLAEQLVAAMPADAQVAKLEVAGAGFINIRLASGARHAAATRVLA
                     EGTAYGRARAAKPEQVMVEFVSANPTGPLHVGHGRQAALGDAIAALFEAQGHAVTREY
                     YYNDAGAQIDKLAWSVQARAQGIGPGQPGWPEEGYAGEYVGEIAKQVADPGDLGAVRR
                     QAVACLRQEQDEDLRAFGVKFDVYTLESGLYTDGRVERAVQALVASGKTYEKDGALWL
                     RTTDYGDDKDRVVRKSDGSHTYFVPDVAYHLAKWERGFRKVVNVQGTDHHSTVTRVRA
                     GLQALGLGIPQGYPDYVLHSLVKVMRGGEEVKISKRAGSYVTLRDLIDWVGRDAVRFF
                     LVSRRADADFVFDVDLARAQSDENPVYYVQYAHARVCSVLRQWGGDASSLGDAPLESL
                     AGQREAALLQRLADFPETLAAAARELAPHSVAFYLRDLAGEFHSYYNAERILVEDRAA
                     RTARLALCAAVRQVLANGLALLGVSAPERM"
     gene            4021..4677
                     /locus_tag="VFB53_03695"
     CDS             4021..4677
                     /locus_tag="VFB53_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZN85869.1"
                     /translation="MSAVPHATARKGTGGGFMLGMFVGLLIGLAIALGVALVLNKTPI
                     PFMSRDKPLAEEGKPAGKPPAIAGLPQGGPAEAMAPVPKPAAKAADKPRFDFYKILPG
                     GEEPVSDRELRERLRAAAAAKSAPAAVPASQDVYFIQAGAFQNPADADNQKARLAILG
                     FESNVEPTNIPDKGTFYRVRLGPYTKVDELNKVRQALAQNGIDANLVKLKDPASAKAN
                     "
     gene            4697..5314
                     /locus_tag="VFB53_03700"
     CDS             4697..5314
                     /locus_tag="VFB53_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZN85870.1"
                     /translation="MLVVALGLAPLGALAQPRALSPGVDYTELKPGQPVDAQGKIEVL
                     EFFWYGCPHCYTLEPMLEKWIARLPADVLFRRVPAVLNPGWGNESAVYYTFEALGVLD
                     KLHRPFFDAIHRDRLTTRDPVALAQWLAKNGVDTKKYQEALKSFGVQSKVRRAAQISA
                     AYRIDGTPALAVHGRYTVSAEQGRTQSGMLAIADGLIALARKDAK"
     gene            5317..5946
                     /locus_tag="VFB53_03705"
     CDS             5317..5946
                     /locus_tag="VFB53_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZN85871.1"
                     /translation="MRTLLPGLALLFALSADAQVAPPVAGRDYRTLEPARLTAPGRLE
                     IIEFFYYGCPVCYEAQPHITRWRQAAGPDVHWRRVPAPNPDSWESLARTFYSLEALGA
                     LERLHWPLYDNHHFDDRRLDEEANLLGWVAANGIDAGRFKEIWHSVETRQQVEFARRT
                     LGIYEVRGVPSFVVDGRYLTSARMAGGVKEAMAVLDYLVERVRKERAAK"
     gene            5951..6715
                     /locus_tag="VFB53_03710"
     CDS             5951..6715
                     /locus_tag="VFB53_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85872.1"
                     /translation="MRRIVITGASAGIGEALARHYAAPDTVIGLIARRREPLQALAAA
                     LPGRCEIHVLDVTDGAALERAAREFCTRHGTPDLVIANAGVSIGTHGDDAADVAKLRR
                     VLDVNVVGLAATLAAFAPAMRAAGRGTLCGIASVAGFRGLPGAGAYSASKAAAIKWLE
                     ALRAELAGSGVSVVTICPGYIDTPMTQVNRYRMPFLLTADEAARRFARAIAARRRLVV
                     IPWQMALVSVLLRLAPAWLYDRFAARAPRKPRDLPL"
     gene            6715..7440
                     /locus_tag="VFB53_03715"
     CDS             6715..7440
                     /locus_tag="VFB53_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZN85873.1"
                     /translation="MRIVSLVPSLTELVCELGLAERLVGRTGFCVHPATIVQGIPKVG
                     GTKTVNLAKIRALAPTHVVLNKDENLRETAEALAAFVPNLVITHPLAPEDNLALYRQF
                     GGTFGKEREAEDLCARFESALQAARKRKYPERKILYLIWKDPWMTVSRDTYISRTLGL
                     FGMRTMPQAAADRYPKLDDVGAADVDEVLLSSEPYRFTAKHIAEVETLTGKPARLVDG
                     EMTSWYGPRAIAGLAYLAAFAEK"
BASE COUNT         1178 a         2703 c         2585 g         1083 t
ORIGIN      
        1 tcggacacgg cattgcgcca aaaacaccgg tcgaaagcgt tgccgcgctc gtggacgaag
       61 tacgcgctta cagccggaaa atgcgcgccg cagctgaaac aaacgcttga cttatgcaca
      121 aacacgcgtt ctcacaatga aaaatagacc tttcggagga ttctccccgc agagcgtcgt
      181 aacctgttga caaatctaga gaataatcgc ccttccccgg agggagcctg gtcccgccgg
      241 gtgccggtga cccgtgtgac accggcatga caggcttatt cacattctta tccacacccc
      301 cggtgcacaa ctaaaaaatg ccttttcgcg gcaagggtta ccagcacaat cgcgaagctt
      361 cggatcaact cgaaaaagta actccttgac gatgcaagtg ctgcgcgtcg cgctcgacgt
      421 accgctggcg acgctgttcg attattcgcg gccggactca ctcgaggtcg cggccggcga
      481 tcgcgtggtg gtgccgttcg gtccgcgttc gcgcgtcggc gtggtgatcg aaaagggcga
      541 aggcagcgcg atcgccgccg aaaagctcaa accggtgtcg cgcgtgctcg cggacacccc
      601 gcgcctgccg tcgcagtggc tcgagctgat gcgcttcctc gccacctact accagcggcc
      661 gctcggcgaa acggtgatcg gcgcgctgcc gccgcgcctg cgatcgctca agccgctgcc
      721 gaaggcggtg cgcgaggccg cggcgccgca gccggcctcg ccgcgcttcg tcggcagcca
      781 taccccgacc gccgaccagg cgcaggcgat cgagcgcatc ggcggcgcgc tcgggcgctt
      841 cgagccgttc ctgctgcatg gcatcaccgg cagcggcaag accgaggtct atctgcggct
      901 gatcgcgcgc gtgctcgagc agggcaacca ggcgctggtg ctggtgcccg agatcagcct
      961 cacgccgcag ctcgagtcgc gcttccgcca cgcgttcccg caagcccata tcgtgatgct
     1021 gcacagcgcc ctgcaggatg tgccgcgcac cgccgcatgg ctggcggctg cgcgcggcga
     1081 ggccggcatc gtgctcggca cgcgcctcgc cacgctaacc ccgctgccgc gcctcgccct
     1141 ggtggtggtc gacgaggagc acgacacctc gttcaagcag caggaggggc tgcgctattc
     1201 cgggcgcgac gcggcagtga tgcgcgccaa gttcgccgcc tgtcccgtgg tgctgggcac
     1261 cgcaacgccg tccctggaga cctggctcaa cgcgctcgtc gggcgctacg cgcgcgtctc
     1321 cctgcccgag cgcgcggtgc cgggcgccag gctgccgatc gtgcgcacgc tggacctgcg
     1381 ccgcgagccg gcgctgcacg gcttcgcgcc cgcgctcatc gccgcgatcg gcgcgcgcct
     1441 ggagcgccgc gagcagagcc tggtgttcat caaccggcgc ggctacgcgc cggtgctcgc
     1501 ctgtcacgcc tgcggctgga cctccggctg cgagcgctgc accgcgcgca tggtgctgca
     1561 caagaccgac cggcgcctgc gctgccacca ctgcggggcg gagagcgcga ttccgcgcgc
     1621 ctgccccacc tgcggcaacg tggatctcaa ggcgatgggc cgcggcacgc agcgcgtgga
     1681 ggagacgctc gaggcgcgct tcccgcaggc gcgcgtggtg cgcatcgacc gcgacagcgc
     1741 gcgccggcgc agcgacctcg cgcgcaccct ggagggcatc ggccgcggcg aggcggacat
     1801 catggtcgga acacaactgc tcgccaaggg ccacgacttc cccctgctca ccctggtcgg
     1861 cgtgctggac gccgacggcg cgctgctgtc caccgactac cgcgctgccg agcggctgca
     1921 cgccacgctc gcccaggtcg ccggccgcgc cggccggcgc gagcagccgg gcgaggtgct
     1981 ggtgcaaacg agctacccgc ggcatccgct ctacgccgcg ctcgccagcc acgacttcgc
     2041 cgccttcgcc gccgcgcagc tcgccgagcg cgaggcggcg ggcttcccgc cgtacgtgca
     2101 cgaggcggcg ctgcgcgccg aggcggacaa gctcgccctg gcgatgcagt tcctcgacga
     2161 cgcggcgcga ctggtgcccg tgcccgacga agtgcgggtc tacgaccctg tgccgcacgt
     2221 cattacgcgc cgcgcggaca tggagcgcgc ccagctgctg gtgcagtcgg cgtcgcggcc
     2281 ggcgctgcag ggcttcctcg ccgcctggag cgagcgcctg ttcgccaccg ccccgcgcaa
     2341 cgtgcgctgg cacctggacg tcgatcccat cgagttcgac tgaactataa ttcgcgcccc
     2401 ccgcatggac atcaaaaccc atcttgccgc gctgttcggg caggcgctgg cgaaaatcgc
     2461 gccggaagcg gcggacgccg aagtgctcat cgagcggccg cgcgatgcgt cgcacggcga
     2521 tttcgcctgc aacctcgcct tgcagctcgc caagcggctg aagcgcagtc cgcgccagct
     2581 cgccgagcag ctggtggccg ccatgcccgc ggacgcgcag gtggcgaagc tggaagtggc
     2641 gggcgccggc ttcatcaaca ttcgcctcgc atcgggcgca aggcatgctg cggcgacccg
     2701 cgtgctggcc gaaggcaccg cctacggccg cgcgcgcgcc gccaagccgg aacaggtgat
     2761 ggtggagttc gtctctgcca accccaccgg cccgctgcat gtcggccatg ggcggcaggc
     2821 cgcgctcggc gacgccatcg ccgcgctgtt cgaggcccag ggccacgcgg taacgcgcga
     2881 gtactactac aacgacgccg gcgcgcagat cgacaagctc gcctggtcgg tgcaggcgcg
     2941 cgcgcagggc atcgggccag ggcagcccgg ctggccggag gaaggctacg ccggcgaata
     3001 tgtcggggaa atcgccaagc aggtcgccga ccccggcgac ctcggcgcgg tgcgccggca
     3061 ggcggtcgcc tgcctcaggc aggagcagga cgaggacctc agggccttcg gcgtgaagtt
     3121 cgacgtgtat accctcgaat ccggcctgta caccgacggc agggtcgagc gggcggtgca
     3181 ggcgctggtg gcgagcggca agacctacga gaaggacggt gcgctgtggc tgcgcaccac
     3241 cgactacggc gacgacaagg accgcgtggt gcgcaagtcc gacggcagcc acacctattt
     3301 cgtgcccgat gtcgcctacc acctcgccaa gtgggaacgc ggcttccgca aggtggtcaa
     3361 cgtacagggg accgatcacc acagcaccgt gacgcgggtg cgcgccgggc tgcaggcgct
     3421 cggcctgggc atcccgcagg gctaccccga ctacgtcctg cacagcctgg tgaaagtgat
     3481 gcgcgggggc gaagaggtga agatctccaa gcgcgcgggc agctacgtca cgctgcgcga
     3541 cctgatcgac tgggtcgggc gcgacgcggt gcgctttttc ctcgtgtcgc gccgcgcgga
     3601 cgccgatttc gtattcgacg tcgacctggc gcgcgcgcag tcggacgaga acccggtgta
     3661 ctacgtgcag tacgcgcacg cccgcgtgtg cagcgtgctc cggcaatggg gcggcgacgc
     3721 gtcgtcgctc ggcgacgcgc cgctcgagag cctcgcgggg cagcgcgaag cggcgctcct
     3781 gcagcgcctt gccgacttcc ccgaaacgct cgccgccgcg gcgcgcgaac tggcgccgca
     3841 ctcggtggcc ttctacctgc gcgacctcgc gggcgagttc cacagctact ataatgccga
     3901 gcgcatcctg gtcgaggaca gggcggcgcg cacggcgcgc ctggcgctgt gcgccgcggt
     3961 ccgccaggtg ctcgccaacg ggctcgcgct gctgggcgtg agcgctccgg agaggatgtg
     4021 atgtcggctg taccccacgc aacggcacgc aagggcacgg gcggcggctt catgctcggc
     4081 atgttcgtcg gcctgctgat cggcctcgcc atcgcgctcg gcgtcgcgct cgttctcaac
     4141 aagacgccga tcccgttcat gagccgcgac aagccgctgg ccgaagaggg caagcccgcc
     4201 ggcaagccgc cggcgatcgc cgggctgccg caaggcgggc ccgccgaagc catggccccg
     4261 gtgcccaagc ccgccgccaa ggcggcggac aagccgcgct tcgacttcta caagatcctg
     4321 ccggggggcg aggagccggt gagcgaccgc gagctgcgcg agcgcctgcg cgcggcggcc
     4381 gcggcgaaga gcgcgcccgc agcggtcccc gccagccagg acgtgtactt catccaggcg
     4441 ggggcgttcc agaacccggc ggatgccgac aaccagaagg cgcggctcgc gatcctcggg
     4501 ttcgaatcga acgtcgagcc caccaacatt cccgacaagg gcacgttcta ccgcgtgcgc
     4561 ctgggcccgt acacgaaagt cgacgaactg aacaaggtgc gacaggcgct ggcgcagaac
     4621 gggatcgacg ccaacctggt caagttgaag gaccccgctt ccgcaaaggc gaactgaaat
     4681 gaatccgcgt cgtcgcatgc tggtcgttgc actcggactc gcgccgctcg gcgccctcgc
     4741 gcaaccgcgc gcgctgtcgc ccggcgtgga ctacaccgaa ctgaagcccg gccagcccgt
     4801 ggacgcccag ggaaagatcg aagtgctcga attcttctgg tacggctgcc cgcactgcta
     4861 cacgctggag ccgatgctgg agaagtggat cgccaggctg cccgccgacg tcctgttccg
     4921 ccgcgtgccg gcggtgctga accccggctg gggcaatgaa tcggcggtgt actacacctt
     4981 cgaggccctc ggcgtgctgg acaagctgca tcgcccgttc ttcgacgcca tccaccgcga
     5041 ccgcctgacc acgcgcgatc cggtcgcgct tgcccaatgg ctggcgaaga acggcgtcga
     5101 caccaagaag taccaggaag cgctcaagtc cttcggcgtg caaagcaagg tgcgccgcgc
     5161 cgcgcagatc tccgcggcct accgcatcga cggcacgccg gcgctggcgg tgcacggccg
     5221 ctacacggtg agcgccgaac agggacgcac ccagtccggc atgctcgcca tcgccgacgg
     5281 cctcatcgcc ctcgcgcgca aggacgcgaa atagccgtgc gcacactcct gcctggcctg
     5341 gccctgctgt tcgccctgag cgcggacgcg caggttgcgc cgcccgttgc ggggcgcgat
     5401 taccggacgc tggaaccggc gcggctgacc gcgcctgggc gcctggagat catcgagttc
     5461 ttctactacg gctgcccggt gtgctacgag gcgcagccgc acatcacgcg ctggcggcag
     5521 gcggccgggc ccgacgtgca ctggcggcgc gtgccggcac ccaatcccga cagctgggag
     5581 tcgctcgcgc gcactttcta cagcctggag gcgctgggcg cgctcgagcg cctgcactgg
     5641 ccgctctacg acaaccacca tttcgacgac cggcgcctgg acgaggaagc caacctccta
     5701 ggctgggtcg cggccaacgg catcgacgcc ggccgcttca aggagatctg gcattccgtg
     5761 gaaacgcgcc agcaggtcga gttcgcacgc cgcacgctcg gcatctatga ggtgcgcggc
     5821 gtgccgagct tcgtggtgga cggccgctac ctcacctcgg cgcgcatggc ggggggcgtc
     5881 aaggaagcca tggcggtgct cgactacctg gtggagcgcg tgcgcaagga acgcgcggca
     5941 aagtagcgcc gtgcgccgga ttgtcatcac cggcgcgtcc gcaggcatcg gcgaggcgct
     6001 ggcccgccac tacgccgccc ccgataccgt catcggcctg atcgcgcgcc ggcgcgagcc
     6061 gctgcaggcg ctagccgcag cgctgcccgg ccgctgcgag atccacgtcc tcgacgtcac
     6121 tgacggcgcc gcactggagc gcgccgcgcg cgagttctgc acgcgccacg gcacccccga
     6181 cctggtcatc gccaacgccg gcgtgtcgat cggcacgcac ggcgacgacg cggcggacgt
     6241 ggcgaagctg cgccgggtgc tcgacgtgaa tgtcgtcggg ctggcggcca cgctggccgc
     6301 cttcgcgccg gcgatgcgcg ccgcggggcg cggcaccctc tgcggcatcg cgagcgtcgc
     6361 cggttttcgc ggcctgccgg gggccggcgc ctacagcgcg tccaaggccg ccgccatcaa
     6421 gtggctggag gcgctgcgcg ccgaactcgc cggcagcggc gtgtcggtgg tgacgatctg
     6481 cccgggctac atcgatacgc cgatgacgca ggtgaaccgc taccgcatgc cgttcctgct
     6541 caccgcggac gaggcggcgc ggcgcttcgc gcgcgccatc gcggcgcggc gccgcctggt
     6601 ggtgattccc tggcagatgg cgctcgtctc ggtgctgctg cgcctggcgc ctgcctggct
     6661 ctacgaccgc ttcgccgcgc gcgcgccgcg caagccgcgc gacctgccgc tctgatgcgc
     6721 atcgtctcgc tggtgccctc gctcacggaa ctggtgtgcg agctggggct ggccgagcgc
     6781 ctcgtcggcc gcacgggatt ctgcgttcac ccggcgacta tcgtgcaggg aatcccgaaa
     6841 gtgggcggca ccaagacggt gaacctggcg aagatccgcg ccctcgcccc cacgcacgtc
     6901 gtgctcaata aggacgaaaa cctcagggag acagcggagg cgctggctgc gttcgtaccc
     6961 aacctcgtca tcacccaccc gctggcgcca gaggacaacc ttgcgctgta tcgccagttc
     7021 ggcgggacct tcggcaagga acgcgaggcg gaagaccttt gcgcccggtt cgagtccgcc
     7081 ctgcaggcgg caaggaaacg caaatacccg gagcgaaaga tcctctatct catctggaaa
     7141 gacccctgga tgacggtgtc gcgcgacacg tacatttcca ggactctcgg cctgttcgga
     7201 atgcgcacga tgccgcaggc tgccgccgac cgctatccga agctcgacga cgttggcgct
     7261 gcggatgtgg acgaggtgct gctctcctcc gagccttacc gattcacggc aaagcacatc
     7321 gccgaggtcg aaacgctcac cggcaagccg gcgcgcctgg tcgatggcga gatgacttcc
     7381 tggtacgggc cgcgcgccat cgccggcctc gcctacctcg ccgccttcgc cgaaaaatag
     7441 ggtctgaccc tattttcttg ttacgtagga gccgggcgcg tcctcgatgg cgttggacgg
     7501 gatgcgggcg gggaccttgt cgccgccgtt ctgctgctcg atccagcgc
//
LOCUS       DASRIE010000080         6642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3747707, whole genome shotgun sequence.
ACCESSION   DASRIE010000080 DASRIE010000000
VERSION     DASRIE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747707"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFB53_03720"
     CDS             <1..484
                     /locus_tag="VFB53_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZN85874.1"
                     /translation="VRVGGDKFDEAIINYIRRNYGMLIGETTAETIKKQIGSAFPGSE
                     VREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVGAVKSALEQTPPELGADIAE
                     KGMVLTGGGALLRDLDRLFMEETGLPVIVADDPLTCVVRGSGKALEQMEHFGPIFTND
                     "
     gene            525..1439
                     /gene="mreC"
                     /locus_tag="VFB53_03725"
     CDS             525..1439
                     /gene="mreC"
                     /locus_tag="VFB53_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZN85875.1"
                     /translation="MEHSPPPFFLRGPAPLVRLAFFASLSLTLLVLDARFRYAESIRS
                     VLALAVYPIQQLARLPVELAEGVAGHFVSQAQLREENEALRVRLLAAAQDAQRFASSD
                     AEAAQLRRLIGAAERAEWKSMPAEILYAGRDPYSRKVILDRGTQHGVRAGSPVLDETG
                     VIGQLTRAHAFVSEVTLLTDKDQAIPVQVLRNGLRAIAFGGGGSGLLELRYMAANADV
                     EGGDQLVTSGLDGTYPPGLPVASVIRVERDATHTFARVLAQPTAGIERGRYALVLSGE
                     ARLPPYPESAAGKDAKPRRAKRKDADGS"
     gene            1429..1947
                     /gene="mreD"
                     /locus_tag="VFB53_03730"
     CDS             1429..1947
                     /gene="mreD"
                     /locus_tag="VFB53_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZN85876.1"
                     /translation="MAPERPHHILLPVRTGTIVASFAAALLLNFLPWPNLALAPDFVA
                     LALAFWCVRQPRLVGLASAWGLGLLVDAGNGVLLGQHALAYSALAFFAIALSRRILWF
                     DAWGQMLHVLLMLEGAQLVALGVRLATGAEFPGWPLFLGPLAATLLWPAVTWLLLIPQ
                     RRPMAADANRPL"
     gene            1944..3917
                     /gene="mrdA"
                     /locus_tag="VFB53_03735"
     CDS             1944..3917
                     /gene="mrdA"
                     /locus_tag="VFB53_03735"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZN85877.1"
                     /translation="MSFAELKNHQIELRAFQLRLGVAGFFVLAAFGLLLARFVWLQVL
                     QHDAYRAKAEENRIAVVPLAPSRGLILDRNGEVLARNYLAYTLEIFPARVRNLEGTIA
                     QLAEYVDIQPRDRARFRKLLQETRNAESLPIRTRLSDEEVARIAANAWRFTGVDIRAR
                     LFRQYPHGDVASHAIGYIGRITTQDLERLEQIEVGANYRGTDYIGRSGIEASYQQELH
                     GTTGLERVEIDAAGRGIRSLSRSAALAGNNIRLTLDIRLQEVAERAFGGRRGALVALE
                     PATGGVLAFVSKPGYDPNLFVDGIDPQSWETLNTSPERPLNNRAIAGLYPPGSTFKPF
                     MALAALDLGKRTPRSTIHDPGYFVFGDRRFRDSKAGGHGTVDLHKSIVVSSDTYYYML
                     ANDMGIDAIAGFMKRFGFGSRTGIDLEGEVAGVLPSPEWKQARFSRPEQKKWYAGETI
                     SIGIGQGYNAYTPLQLALATATLANNGDMFRPHLVSTVDNARTGERRTVEPQLVRQLQ
                     LRPEHVAFVKQAMAGVAREGTSARSFAGAQYTSGGKTGTAQVVAMKQNEKYEEDKVAE
                     HLRDHSLYIAFAPLESPRIALAVLVENGGFGARAAAPIARIVLDYFLLGKLPAAPKPA
                     APRPAAPRPAAAAPEYAPEDVPGGAPEDDAAND"
     gene            3917..5029
                     /gene="rodA"
                     /locus_tag="VFB53_03740"
     CDS             3917..5029
                     /gene="rodA"
                     /locus_tag="VFB53_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZN85878.1"
                     /translation="MEIAGYPIRRLRARLVEGIDTPLFVLALALAAIGLAALYSASYG
                     NASRVAAQLANLALALMAMWALAQVAPQTLARFALPAYIVGIALLLAVALFGEVVNGA
                     RRWLNLDLVRFQPSEMMKIAVPLMLAWYFHRREGSIRLRDFALAGLLLAAPVALVARQ
                     PDLGTALLIAAAGFYVIFFAGISWKLLAGLAVLGLAALAPLWGMLHDYQRRRILTLLD
                     PSTDPLGSGYHIIQSTIAVGSGGIAGKGWLNGSQTHLEFIPERHTDFIFAVYSEEFGL
                     IGNVVLLALYALLVARGLAIAAGAPTLFARLLAGAVTMIFFTYAFVNMGMVAGILPVV
                     GVPLPFISYGGTALMTLFVGIGILMSIHRHRKLVQT"
     gene            5209..6522
                     /gene="aceA"
                     /locus_tag="VFB53_03745"
     CDS             5209..6522
                     /gene="aceA"
                     /locus_tag="VFB53_03745"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZN85879.1"
                     /translation="MHKQDQEIAALEQEWARNPRWQGLKRGYGAADVVRLRGSVRIEH
                     TLAKLGAEKLWKKCSEMPFVNSLGALTGNQAMQQVKAGVPAIYLSGWQVAADANDSLS
                     MYPDQSLYATTSVPTVVKRINNALARQDQIQTMEGKGDVDWYAPIVADAESGFGGVLN
                     AFELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVMGV
                     PTVLLARTDAEAADIVTTDVDDNDKPFLTGERTSEGFYRTRKGFDQALSRGLAYAAYA
                     DMVWCETGTPDLAFARKFAEGIRRQYPGKMLAYNCSPSFNWKRNLDDAAIAKFQRELG
                     AMGYKFQFITLADFHALNYSMFELAYGYARSNMSAFVELQEREFAAAEKGFTAVKHQR
                     EVGTGYFDEITQVVTAGKASTTALHGSTEDEQFFDSQKKPKLAAA"
     gene            6480..>6642
                     /locus_tag="VFB53_03750"
     CDS             6480..>6642
                     /locus_tag="VFB53_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HZN85880.1"
                     /translation="MLRLAEKAEARRGVDFAAEVARAILAGFDRHYRLFRQAAVDAQR
                     LYERAAWREL"
BASE COUNT         1010 a         2399 c         2219 g         1014 t
ORIGIN      
        1 ggtgcgcgtc ggcggcgaca agttcgacga agcgatcatc aactacatcc gccgcaacta
       61 cggcatgctg atcggcgaga ccaccgccga gaccatcaag aagcagatcg gctccgcctt
      121 cccgggcagc gaggtgcgcg agatggaagt gaagggccgc aacctcgccg agggcatccc
      181 gcgcagcttc accatcagct ccaacgaaat cctcgaagcg ctcaccgagc cgctcaacca
      241 gatcgtcggc gcagtcaaat ccgcgctgga gcagaccccg ccggaactcg gcgccgacat
      301 cgccgagaag ggcatggtgc tcaccggcgg cggcgcgctg ctgagggacc tggaccgcct
      361 gttcatggaa gagaccggcc tgcccgtgat cgtcgccgac gacccgctca cctgcgtggt
      421 gcgcggctcc gggaaggcgc tcgagcagat ggaacacttc gggccgatct tcaccaatga
      481 ctgattgagg ggggcgccgc cggcgcgcct ttgccctgcc cgagatggag cactctcccc
      541 cgccgttctt cctccgcgga cccgctcccc tcgtccgcct cgcgttcttc gcttcgctgt
      601 cgctgaccct gctggtgctc gacgcgcgct ttcgctacgc cgagagcatc cgcagcgtac
      661 tggcgctcgc cgtctacccg atccagcagc tggcgcggct gccggtggag ctggccgagg
      721 gcgtcgccgg ccacttcgtc agccaggcgc agctgcgcga ggagaacgag gcgctgcgcg
      781 tgcgcctgct cgccgccgcg caggacgcgc agcgcttcgc gtcgtccgac gccgaagccg
      841 cgcagctgcg ccgcctgatc ggcgcggccg agcgcgccga gtggaagtcg atgcccgccg
      901 agatcctgta cgcggggcgc gacccgtact cgcgcaaggt gatcctcgac cgcggcacgc
      961 agcacggcgt gcgcgcgggc agcccggtgc tggacgagac cggggtgatc ggccagctga
     1021 cgcgcgcgca cgccttcgtc tcggaggtca cgctgctcac cgacaaggac caggcgatcc
     1081 cggtgcaggt gctgcgcaac ggcctgcgcg ccatcgcctt cggcggcggc ggctccggcc
     1141 tgctggagct gcgctacatg gcggccaacg ccgacgtcga gggcggcgac cagctggtca
     1201 cctccggcct ggacggcacc tacccgccgg ggctgccggt ggcgagcgtg atccgcgtgg
     1261 agcgcgacgc gacccacacc ttcgcgcgcg tgctggcgca gcccaccgcc ggcatcgagc
     1321 gcggccgcta cgcgctggtg ctctcgggcg aggcgcgcct gccgccctat cccgagtccg
     1381 ccgccggcaa ggatgccaag ccgcggcgcg cgaagcgcaa ggacgccgat ggctcctgag
     1441 cgcccgcatc acatcctcct gccggtgcgc acgggcacca tcgtggcctc gtttgccgcc
     1501 gcgctgctgc tcaacttcct gccctggccc aacctcgccc tggcgccgga cttcgtcgcg
     1561 ctggcgctcg ccttctggtg cgtacgccag ccgcgcctgg tgggcctcgc cagcgcctgg
     1621 gggctcggcc tgctggtgga cgccggcaac ggcgtccttc tcgggcagca cgcgctcgcc
     1681 tactcggcgc tcgcgttctt cgccatcgcc ctgtcgcgcc gcatcctgtg gttcgacgcc
     1741 tggggccaga tgctgcacgt gctgctgatg ctggaaggcg cgcagctggt ggcgctcggc
     1801 gtgcgcctcg cgacgggcgc cgagttcccg ggctggccgc tgttcctggg gccgctggca
     1861 gcgaccctgc tctggccggc ggtcacctgg ctgctcctga tcccgcagcg ccgcccgatg
     1921 gcggcggacg ccaaccggcc gctatgagct tcgccgagct gaagaaccac cagatcgagc
     1981 tgcgcgcctt ccagctgcgc ctgggggtcg ccggcttctt cgtgctcgcc gccttcggcc
     2041 tgctgctggc gcgcttcgtc tggctgcagg tgctgcagca cgacgcctac cgcgcgaagg
     2101 ccgaggagaa ccgcatcgcc gtggtgccgc tggcgcccag ccgcggcctg atcctggacc
     2161 gcaacggcga agtgctggcg cgcaactacc tcgcctacac gctggagatc ttcccggcgc
     2221 gcgtgcgcaa cctggagggc accatcgccc agctcgccga gtacgtcgac atccagccgc
     2281 gcgaccgcgc gcgcttcagg aagctgctgc aggaaacgcg caatgccgag agcctgccga
     2341 tccgcacccg gctctccgac gaggaggtgg cgcgcattgc cgccaacgcc tggcgcttca
     2401 ccggcgtcga tatccgcgcg cggctgttcc gccagtatcc gcacggcgac gtcgcctcgc
     2461 acgccatcgg ctacatcggg cgcatcacca cgcaggacct ggagcggctc gagcagatcg
     2521 aggtcggggc caactaccgc ggcacggact acatcggccg ctccggcatc gaggcgagct
     2581 accagcagga gctgcacggc accaccggcc tggagcgcgt cgagatcgat gccgccgggc
     2641 gcggcatccg cagcctgtcg cgcagcgccg cgctggcggg caacaacatc cgcctgacgc
     2701 tggacatccg gctgcaggag gtggccgagc gcgccttcgg cgggcgccgc ggcgcgctgg
     2761 tggcgctcga gccggccacc ggcggcgtac tcgctttcgt ctccaagccg ggctacgacc
     2821 cgaatctgtt cgtcgacggc atcgacccgc agagctggga gacgctgaac acctcgcccg
     2881 agcggccgct caacaaccgg gccatcgccg gcctgtaccc gccggggtcc accttcaagc
     2941 cgttcatggc cctcgccgcg ctcgacctgg gcaagcgcac gccgcgcagc accatccacg
     3001 acccgggcta tttcgtgttc ggcgaccggc gcttccgcga ttccaaggcc ggcggccacg
     3061 gcacggtgga cctgcacaag tccatcgtcg tgtcctccga cacgtactac tacatgctcg
     3121 ccaacgacat gggcatcgac gccatcgccg gcttcatgaa gcgcttcggc ttcggttcgc
     3181 gcaccggcat cgatcttgaa ggcgaagtcg cgggcgtgct gccttcgccg gaatggaagc
     3241 aggcgcgctt ttcccggccg gagcagaaga agtggtacgc cggcgaaacc atctccatcg
     3301 gcatcggaca gggctacaac gcctacacgc cgctgcagct ggcgctcgcc acggcgaccc
     3361 tcgccaacaa cggcgacatg ttccggccgc acctggtgtc gaccgtggac aacgcgcgca
     3421 ccggcgagcg ccgcaccgtc gagccgcagc tggtgcgcca gctgcagctg cgccccgagc
     3481 acgtcgcctt cgtcaagcag gcgatggccg gggtcgccag ggaggggacc agcgcgcgct
     3541 ccttcgccgg cgcgcagtac accagcggcg gcaagaccgg caccgcgcag gtggtggcga
     3601 tgaagcagaa cgagaaatac gaggaagaca aggtcgccga gcacctgcgc gatcattcgc
     3661 tgtacatcgc tttcgcgccg ctggagagtc cgcgcatcgc cctcgccgtg ctggtggaga
     3721 acggcggctt cggcgcgcgc gccgcggcgc ccatcgcgcg catcgtgctg gactacttcc
     3781 tactcggcaa gctgccggcg gcgcccaagc cggctgcgcc caggccggcg gcgcccaggc
     3841 cggcggcggc cgcgcccgag tacgcacccg aggatgtgcc cgggggcgcg cccgaggacg
     3901 acgccgcgaa cgactgatgg aaatcgccgg ttatccgatc cgccgcctgc gcgcgcgcct
     3961 cgtcgagggc atcgacacgc cgctgttcgt gctggcgctg gcgctcgccg ccatcggcct
     4021 ggcggccctg tattcggcga gctacggcaa cgcctcgcgc gtcgcggcgc agctcgccaa
     4081 tctcgcgctg gcgctgatgg cgatgtgggc gctggcgcag gtcgcgccgc agacgctggc
     4141 gcgcttcgcg ctgccggcct acatcgtcgg catcgcgctg ctgctcgccg tggccctgtt
     4201 cggcgaggtg gtgaacggcg cgcgccgctg gctgaatctg gacctggtgc gcttccagcc
     4261 ctcggagatg atgaagatcg ccgtgccgct gatgctcgcc tggtacttcc atcgccgcga
     4321 gggttccatc cgcctgcgcg atttcgcgct cgccggcctg ctgctcgctg cgccggtggc
     4381 gctggtggcg cgccagcccg acctcggcac cgccctgctc atcgccgccg cgggcttcta
     4441 cgtcatcttc ttcgccggca tcagctggaa gctcctcgcc gggctggccg tgctggggct
     4501 cgccgcgctc gcgccgctgt ggggcatgct gcacgactac cagcgccggc gcatcctcac
     4561 cctgctcgat ccctccaccg atccgctcgg ctccgggtac cacatcatcc agtccaccat
     4621 cgcggtgggc tcgggcggca tcgccggcaa gggctggctg aacggctcgc agacgcacct
     4681 cgaattcatc cccgagcgcc acaccgactt catcttcgcc gtgtactccg aggagttcgg
     4741 cctgatcggc aacgtcgtgc tgctggcgct ctacgcgctg ctcgtcgcgc gcggactggc
     4801 gatcgccgcg ggcgcgccga cgctgttcgc gcgcctgctc gccggggcgg tgacgatgat
     4861 tttcttcact tacgccttcg tcaacatggg catggtggcg ggcatcctgc cggtggtcgg
     4921 cgtgccgctg cccttcatct cctacggcgg cacggcgctg atgaccctgt tcgtcgggat
     4981 cggcatcctg atgagcatcc atcgccacag gaagctggtg cagacgtagt tgttgctttg
     5041 cagcactatt ctgaaattga tctccgcatc gtgaaatatc gatgccgtaa gtcgttgtaa
     5101 atccttgttt aagtaccatt tcacatctcg caatggcgtt gttgctgcgc gccaagcgcg
     5161 tggctatcgt cggggtcgga ccaattcacc cccaccgaca ggagcgccat gcacaagcaa
     5221 gaccaggaga tcgccgcact cgagcaggaa tgggcgcgca acccgcgctg gcagggcctg
     5281 aagcgcggct acggcgccgc ggacgtcgtg cggctgcgcg gcagcgtcag gatcgagcac
     5341 acgctggcga agctcggcgc ggagaaactg tggaaaaaat gcagcgagat gccgttcgtg
     5401 aactcgctcg gcgcgctcac cggcaaccag gccatgcagc aggtgaaagc cggcgtgccc
     5461 gcgatctacc tctcgggctg gcaggtggcg gcggatgcca acgattcgct ctccatgtat
     5521 ccggaccagt cgctctacgc gacgacctcg gtgcccacgg tggtgaagcg catcaacaac
     5581 gccctcgcgc ggcaggacca gattcagacg atggagggca agggagacgt tgattggtac
     5641 gcccccatcg tggccgacgc ggaatccggt ttcggcggcg tgctgaacgc cttcgagctg
     5701 atgaaggcga tgatcgaggc cggcgccgcc ggcgtgcact tcgaggacca gctcgccagc
     5761 gtgaaaaaat gcggccacat gggcggcaag gtgctcgttc ccacgcagga agcggtgcaa
     5821 aagctcatcg ccgcgcgcct cgccgccgac gtgatgggcg tgcccacggt gctgctggcg
     5881 agaacggacg cggaggcagc cgatatcgtc accaccgacg tggacgacaa cgacaagcct
     5941 ttccttaccg gagagcgcac ctcggagggc ttctacagga cgcgcaaggg cttcgaccag
     6001 gcgctgtcgc gcggcctcgc ctacgcggcc tacgccgaca tggtgtggtg cgagaccggc
     6061 acgccagatc tcgccttcgc gcggaagttc gccgaaggca tccgcaggca gtacccgggc
     6121 aagatgctcg cctacaactg ctcgccctcg ttcaactgga agcgcaacct cgacgacgcc
     6181 gcgatcgcga aattccagcg cgagctcggc gccatgggct acaagttcca gttcatcacg
     6241 ctggccgact tccacgcgct caactactcg atgttcgagc tcgcctacgg ctacgcgcgc
     6301 agcaacatga gcgccttcgt cgagctgcag gagagggaat tcgccgccgc ggagaagggc
     6361 ttcaccgcgg tcaagcacca gcgcgaggtc ggtaccggct acttcgacga gatcacccag
     6421 gtcgtgaccg cgggcaaggc ctcgacgacc gcgctgcacg gttcgaccga ggacgagcaa
     6481 ttcttcgact cgcagaaaaa gccgaagctc gccgcggcgt agatttcgcc gccgaggtcg
     6541 cgcgcgcgat cctcgcgggc ttcgaccggc actaccgcct gttccgccag gcggcggtgg
     6601 acgcgcagcg cctgtacgag cgcgccgcct ggcgcgagct gc
//
LOCUS       DASRIE010000081        14339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_608882, whole genome shotgun sequence.
ACCESSION   DASRIE010000081 DASRIE010000000
VERSION     DASRIE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608882"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VFB53_03755"
     CDS             complement(<1..1315)
                     /locus_tag="VFB53_03755"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZN85881.1"
                     /translation="MTAVALRFAAIPQAARLALLALAYFAAAKASLSFAIPPGYATAV
                     WLPSGIAVGAVILWGARCWPGLWLGAALANFTINLSVPVAVGIATGNTLEALCAGWLA
                     AGLVDRGTGLQRPEAVFRFAAVAALSSAVAASAGTVSLYLSGSIGDGALVANWYTWWL
                     GDTTGILVAVPCLLAWVGAPQLPRSRMLSHELTAFAVLFLATLLAIFATTPADGAIRT
                     VAFLAMPFFAWAACRYDQRVVSACVLAATGLAIWCTVIGRGPFAGGALNESLLTLQAF
                     TGTGALIALALGALTHDRGRALRALRASHDSLDVAVRAQGAALGASERESARVQALAH
                     VGVWNWDAPSGRISWSDELCRIHGIEPGAFEGGFEAHLAGVDARDRERMRSLLQAALF
                     ECRPWEAMVRIVRRDGAARVLHSFGRVVAGARGRPARIQGFCVDVT"
     gene            complement(1478..3967)
                     /locus_tag="VFB53_03760"
     CDS             complement(1478..3967)
                     /locus_tag="VFB53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN85882.1"
                     /translation="MRQALRMLARDWRAGEVRVLAAALVVAVASITSVAFFADRVSRA
                     LVRDAHQLLGADLVLVSDHRWKREMADEIARRGLQRAEAVNFISMARAGESSLLAGVK
                     AVSQNYPLRGRLRIAPAPNAPDAPAQQGPARGTVWLEERLVTALGLPMGASLRLGTAE
                     FKVAAILTLEPERGTNFFNIAPRLVMHQDDVPATGLVQTGSRVWFYLYAAGEPAAVAA
                     FDAWARARLERGQRVDNLESGRPEVRASIDRAQRFLGLTALLAAILAGVAIALGTRRY
                     VERHLDGCAVMRCFGATQRQLLSLYAAEFLLLGLAACALGCAIGYAAQNAIGAALGTM
                     LRAALPPASPLPALQGILVGVVLLLGFALPPLVQLKNVPAVRVIRREAGAPSTSALAV
                     YAAGIAALGGLLVWQAGDVKLGLTVIGGFGAAVLVFAAVAWAALRLVSAAPVARRLGA
                     RSLALRYGLANLRRHAKGNAVQVASLALGLTAVLLLTFTRNDLVDAWRRSAPPDAPNR
                     FLLGVQPEQAAEVSRFFAARGIAAPELSPMVRGRLIAVNGKPVTEADFGEERAKRLVE
                     REFNLSYAERPPAHNQIVAGSWFAPGARELSVEEGIAGTLGWKLGDELEFAVGGQRLG
                     ARVTSLRKLRWDSMKINFFVIAPSALLADFPASFVSAFRAEDGQEAALNELIKRFPNL
                     TLVDVGAAVKQAQGVIDQLINAVQFVFLFALGAGLLVLYSALVATEDERRREAAVMRV
                     YGASRGQVTGSQRAEFLAMGLVAGVLATLGAALIGQLLARRVFELDLPPSPGLWIAGP
                     IAGVALLSLNAWLSARKVLSASPALTLRDSV"
     gene            complement(3967..4647)
                     /locus_tag="VFB53_03765"
     CDS             complement(3967..4647)
                     /locus_tag="VFB53_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85883.1"
                     /translation="MNRQTSNVLAARGIGKTVKSGDRDLVILRDIGLEVMPGEAVAIV
                     GASGSGKSTLLALLAGLDTPSTGSVELDGASLFELDEDARAELRGRAVGFVFQTFQLL
                     PSLTALENVMLPLELAGRDDAEAAAREILARVGLAERTHHYPKHLSGGEQQRVALARA
                     FAVRPKLLLADEPTGSLDAEAGDAVIALLFQMNREAGTTLVLVTHDEHLAQRCTRIVR
                     LAAGRIVA"
     gene            4628..5218
                     /locus_tag="VFB53_03770"
     CDS             4628..5218
                     /locus_tag="VFB53_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZN85884.1"
                     /translation="MLVCLFICFSSGAWGKTILVYGDSLSAAYGIAEKRGWVALLGER
                     LRRERPDYSVANASISGETSSGGLGRMARALAQHKPAVLVLELGANDGLRGLPVAQMK
                     QNLSAMILQAQKAGARVVLVGMRIPPNYGPEYTREFEAAFADLAQRHRTARVPFLFAG
                     MGEDLALFQPDRIHPTEAAQPILLDNVWKALRPLLK"
     gene            complement(5211..6260)
                     /gene="selD"
                     /locus_tag="VFB53_03775"
     CDS             complement(5211..6260)
                     /gene="selD"
                     /locus_tag="VFB53_03775"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZN85885.1"
                     /translation="MNAPDRIRLTQFSHGGGCGCKIAPAVLSEILASTPIRGLPADLL
                     VGTETSDDAAVYRLSDTQALVATTDFFTPIVDDPYDFGRIAATNALSDIYAMGGKPIM
                     ALAIVGMPLDKLPVSAIQKILEGGESVCAAAGIPIAGGHSIDTLEPIYGLVGLGLVHP
                     EQVKRNSTAQAGDVLILGKPLGVGILSAALKKGKLSEAGYRAMIAATTQLNTPGTALA
                     AMPGVHALTDVTGFGLAGHLLEILRGSKLAAQVAFESLPVIGEALEWAKQGVATGASD
                     RNWQGYGDEVQLPAGAPEWMRKLVSDPQTSGGLLVACAPQAEQAVLAEFARRGFAEAR
                     RIGKFVVGAPRLTIT"
     gene            6280..7332
                     /gene="mnmH"
                     /locus_tag="VFB53_03780"
     CDS             6280..7332
                     /gene="mnmH"
                     /locus_tag="VFB53_03780"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZN85886.1"
                     /translation="MQTALHSVPMQASSLTAFDAIIDARSPAEFAEDHLPGAVSLPVL
                     DDAERARVGTVYKQVSPFAAKKLGAALVARNIGRHLEGALADRPKGWRPLVYCWRGGK
                     RSGAFAHVLREIGWDARALEGGYKAYRQWVVARLAEVPGTLRFRVVHGVTGSGKSRLL
                     KALAQAGAQVLDLEDLAAHRGSVLGNLPDRAQPSQKMFDSRVLHELAACDPGRAVYVE
                     GESKKIGQLQVPEALIQAMRASDCVLLEADVRTRVALLSAEYRHFFTAREALEARLDC
                     LVGLHGRERVQSWKALAAQGAWQELVARLLDEHYDPAYRRSAQRNFTRLPQARVLRVP
                     AADDAAFAGLARELVA"
     gene            7329..7808
                     /locus_tag="VFB53_03785"
     CDS             7329..7808
                     /locus_tag="VFB53_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85887.1"
                     /translation="MRTLLLALACAAAPAAAAPELRAALESGDLICEFDHDKRSLIAD
                     LAGGARASDLLLVYESVRPESAQVLSSSSPGRRPVLVRATARAVHFIERVGPSVRVTT
                     LTRCELHKWKNGARACVRYAAQHAWHFDALAAADPDLAFDRLPAGASPGRCEPWDLD"
     gene            7886..8152
                     /locus_tag="VFB53_03790"
     CDS             7886..8152
                     /locus_tag="VFB53_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN85888.1"
                     /translation="MEIKDLRYFIAVYEEGGFGRAGVLLETVQSNVSARIRRLELFLG
                     VRLFERRHRGVVVTEKGEKLYRHAKRVIALLEETSAAVKGKEAA"
     gene            complement(8154..9212)
                     /gene="senA"
                     /locus_tag="VFB53_03795"
     CDS             complement(8154..9212)
                     /gene="senA"
                     /locus_tag="VFB53_03795"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HZN85889.1"
                     /translation="MLERQLLEARARTDLMTGDLGGERLLGPRLAIVNPPLWEIGHVG
                     WFQERWCLRTVRGGAVASILPEADALYDSSAVAHDRRWDLPLPTLAATRAYLSGVLER
                     VLDRLAREPENAALRYYAQLCVFHEDMHAEAFHYTHQTLGYGDPAGSALIRAGPQADV
                     GLPGGRCTLGVQPGGAFAFDNEKWAHEIELAPFRIAAELVTNAEYLAYVEAGGTAPRY
                     WRKAGQAWQQRRFERWLALAPQEPVLHVSALEAQAYCRWAGRRLPSEAEWEFAATRGA
                     LHTGQAWEWTASDFLPYPGFSPDPYEDYSRPWFGTQRVLRGASFSTPARLARPGFRNF
                     YAPERADMFCGFRTCAAR"
     gene            9237..10202
                     /gene="senB"
                     /locus_tag="VFB53_03800"
     CDS             9237..10202
                     /gene="senB"
                     /locus_tag="VFB53_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HZN85890.1"
                     /translation="MARIAIVTPAGAGTRNGNRHTALRWAAQLRSAGHGVSVDVEWSG
                     KSCALLVALHARRSHGSIARYREAHPHGPLCVALTGTDLYRDLPASREARHSLELADR
                     ILVLQAEALRRLAPAQRRKARVVYQSADSGARRAPPERVFRVAVIGHLREEKDPFRAA
                     AALAELPAAAGIEVVQLGAALSPDMAREARRRMAAEPRYRWLGGVSHARALGWLARSQ
                     LLVVSSRMEGGANVIAEAARAGTPVLASRVDGNIGMLGHAYPGYFRLGDQHALARLIL
                     RAATDPRFYLALRKALAARRARFAPAAERAALLRALAPLLRPRAR"
     gene            complement(10183..11259)
                     /locus_tag="VFB53_03805"
     CDS             complement(10183..11259)
                     /locus_tag="VFB53_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZN85891.1"
                     /translation="MDRRSFLKSAGAAATASALPWRAALAAEGWQAYEIVSRVEILKP
                     SGVSRTWVPLPLSDDTPWHKAVGNAWSGNAAQARVMSDGKYGVGMLVAEWSPGVAEPV
                     IEVTSRFMTRERSVDLSRPGADPRRLSAEEQAFYTAPTELIPTDGIVKQTAQQVTQGA
                     RGDVDKARAIYEWVVDNTFRDPKTRGCGWGDIKSMLESGNLGGKCGDLNAMFVGLARA
                     AGLPARDIYGLRVAPSMYGYRSLGVGSPNVTRAQHCRAEFFAKGYGWVPVDPADVRKV
                     ILEEPPGNLPAGDAKVAAARKRLFGAWEMNWLAYNMGHDIALPGASGPKLSYLMYINA
                     QTGGMMRDQLDPDSVKYAITARAV"
     gene            complement(11524..12453)
                     /locus_tag="VFB53_03810"
     CDS             complement(11524..12453)
                     /locus_tag="VFB53_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZN85892.1"
                     /translation="MPTAFITHADCLKHEMGAGHPERPERLTAIEDQLIASGVGAQLA
                     RHEAPLATDEELERVHPPEYLRAIRDAAPESGRVHLDPDTAMNPHSLHAALRAAGAAV
                     LATDLVLRGAADNAFCSVRPPGHHACRARPMGFCIFNNVAVAARHALAAHGLQRVAIV
                     DFDVHHGNGTEEIFENDAQVLMVSTFQHPFYPYSGTEAPAPNMVNVPLAAGAGSRQFR
                     TAVSEAWIPALDAFRPQLVLFSAGFDAHEEDDMAMLRLSDADYGWVTRQVKAVADRHA
                     QGRMVSMLEGGYALSALGRSVMQHLRVMASLDR"
     gene            12487..13431
                     /locus_tag="VFB53_03815"
     CDS             12487..13431
                     /locus_tag="VFB53_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN85893.1"
                     /translation="MLLKTQIKTQPAPTPVESLVATAGRVILGKETQVRLALACLIAR
                     GHLLIEDLPGVGKTTLAHVLARTLGLHFQRIQFTSDMLPADIIGVSVYERDGGTFKFH
                     PGPIFAQVILADEVNRATPKTQSALLEAMEEHQVTVETETRKLPEPFFVIATQNPSEQ
                     VGTFPLPESQLDRFTMRIELGYPDRDAERALLTGTDRRDLLATLEPCMSPAELVELQG
                     TAQRVHVSAALLDYVQAIVAHTRRSPDYVAGLSPRAALALVHAARAWALLEGRDKVIP
                     EDVQAILPGVAAHRLRPAHDAARRVDVGAQLLAAVPIP"
     gene            complement(13428..>14339)
                     /locus_tag="VFB53_03820"
     CDS             complement(13428..>14339)
                     /locus_tag="VFB53_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZN85894.1"
                     /translation="DEPTLTMNFLVNASPLAGREGKYVTSRQIRERLERETKSNVAMR
                     VEYPDDTDTFIVHGRGELHLTILLENMRREGYEIAVSRPRVVYKEIGGVRSEPFEVLT
                     VDVEEVNQGGVMEELGRRRGELLEMHPDGKGRVRLDYRIPARGLIGFQGEFMTLTRGT
                     GIMSHVFDEYAPARPEDMAERRNGVLVSQDDGVAVAYAIWKLQERGRMFVSPGEPVYE
                     GMIVGIHSRDNDLVVNPIRTKQLTNIRASGKDEAIDLVPPIGLTLEYAVEFIADDELV
                     EITPKSIRLRKRFLRENERRRAERAAA"
BASE COUNT         2102 a         5197 c         5018 g         2022 t
ORIGIN      
        1 cggtgacgtc gacgcagaat ccctggatgc gcgccggcct gccgcgagcg cccgccacca
       61 cgcggccgaa gctgtgcagc acgcgcgccg cgccgtcgcg gcgtacgatg cgcaccatcg
      121 cctcccaggg cctgcactcg aacagcgccg cctgcagcag cgagcgcatg cgctcgcggt
      181 cgcgcgcgtc cacgcccgcc aggtgcgcct cgaagccgcc ttcgaaggcg cccggctcga
      241 tgccgtggat gcggcacagc tcgtccgacc agctgatgcg gcccgatggc gcgtcccagt
      301 tccatacgcc gacatgcgcc agcgcttgca cccgcgccga ctcgcgttcg ctcgcgccca
      361 gcgccgcgcc ctgcgcgcgc accgcgacgt cgaggctgtc gtgcgaggcg cgcaacgcgc
      421 gcagcgcgcg cccacgatca tgcgtcagcg cgcccagcgc gagggcgatc agcgcgccgg
      481 tgcccgtgaa cgcctgcagc gtgagcagcg attcgttcag cgcgccgccg gcgaacgggc
      541 cgcgcccgat taccgtgcac cagatggcga ggccggtggc ggccagcacg cacgcgctca
      601 ccacgcgctg gtcgtagcgg caggcggccc aggcaaagaa cggcatggcg aggaatgcga
      661 ccgtgcggat tgcgccgtcg gccggcgtgg tggcgaagat tgcaagcaac gtggccagga
      721 acaggacggc gaacgcggtc agctcatggc tgagcatgcg gctgcgcggc agctgcggcg
      781 cgccgaccca ggcgagcagg cagggcacgg ccaccaggat gcctgtcgtg tcgcccagcc
      841 accacgtgta ccagttggcg accagcgcgc cgtcgccgat gctcccggac aggtacagcg
      901 aaacggtgcc tgcggaggcg gcgacggcgc tgctcagcgc agcgaccgcc gcgaagcgga
      961 acacggcttc gggccgctgc aagccggtgc cgcggtccac caggccggct gcgagccagc
     1021 cggcgcacag ggcctccagc gtattgccgg tggcgatgcc gaccgcgacc ggcaccgaga
     1081 ggttgatggt gaagttggcc agggcggcgc ccaaccacag gccgggccag cagcgcgcgc
     1141 cccacaggat caccgccccg accgcgatcc cggacggcag ccacacggcg gtcgcgtagc
     1201 ccggcgggat ggcgaacgag agggaagcct tcgccgccgc gaagtatgcg agcgcgagca
     1261 gcgccagcct agcggcctgg ggtattgcgg cgaagcggag agcaacagca gtcactcgag
     1321 cgcgtccctc ccgggtaccg ttcgttgccc ctgcagctat tgtcctcttt ccgggcggaa
     1381 agttccgcct acgcgcggcg agcgtgacct ttgcccacgt cctggtgtgc gataccgcac
     1441 acaaaaggcc atccagcgca tagcagggtc tcccgggcta gacggagtcc ctgagcgtca
     1501 gggcgggcga ggcggagagc accttgcgcg ccgagagcca ggcgttgagc gacagcagcg
     1561 ccacgccggc gatcgggccg gcgatccaca gcccggggct gggcggcagg tcgagctcga
     1621 acacgcgccg cgccagcagc tgcccgatca gggccgcgcc cagcgtcgcc agcacccccg
     1681 ccaccagccc catggcgagg aactccgcgc gctggctgcc tgtcacctgc ccgcgcgagg
     1741 cgccataaac gcgcatcacc gccgcctcgc gccggcgctc gtcttcggtc gccaccagcg
     1801 ccgagtagag caccagcagg cccgcgccga gcgcgaacag gaagacgaac tgcaccgcgt
     1861 tgatcagctg gtcgatgacg ccctgcgcct gcttcaccgc ggcaccgacg tccaccagcg
     1921 tgaggttggg aaagcgcttg atcagctcgt tcagcgccgc ctcctgcccg tcctcggcgc
     1981 ggaacgccga aacgaagctc gccgggaagt ccgccagcag cgcgctcggc gcgatgacga
     2041 agaaattgat cttcatcgaa tcccagcgca gcttgcgcag cgaagtgacg cgcgcgccga
     2101 ggcgctgccc gccgacggcg aactctagct cgtcgcccag cttccagccc agcgtcccgg
     2161 cgatgccctc ctccaccgag agctcgcgcg cgccgggcgc gaaccagctc cccgcgacga
     2221 tctggttgtg cgccggcggc cgctcggcgt aggacaggtt gaactcgcgc tccaccaggc
     2281 gcttggcgcg ctcttcgccg aaatcggctt ccgtcaccgg cttgccgttg accgcaatca
     2341 gccgcccgcg caccatcggg ctgagttccg gcgcggcgat gccgcgcgcc gcgaagaagc
     2401 ggctcacctc ggccgcctgc tcgggttgca cgccgagcag gaagcggttg ggcgcatcgg
     2461 gcggcgcgct gcgccgccag gcgtccacca ggtcgttgcg cgtgaaggtc agcagcagta
     2521 ccgcggtgag gcccagggcg aggctcgcca cctgcacggc gttgcccttg gcatggcggc
     2581 gcaggttcgc caggccgtat cgcagcgcca ggctgcgcgc cccgagccgc cgcgccaccg
     2641 gcgcggccga caccagccgc agcgccgccc aggcaaccgc ggcgaacacc agcaccgcgg
     2701 cgccgaagcc gccgatcacg gtcaggccca gcttcacatc ccccgcctgc cacaccagca
     2761 ggccgcccag cgcggcaatg cccgccgcgt agacggcgag cgcgctggtg ctcggcgcgc
     2821 ccgcctcgcg gcggatgacg cgtaccgcgg gcacgttctt cagctgcacc agcggcggca
     2881 gcgcgaaacc gagcagcagc accacgccga ccaggatgcc ttgcagcgcc ggcagcggcg
     2941 aggcgggcgg cagggccgcg cgcagcatcg tgccgagcgc ggcgccgatg gcattctgcg
     3001 cggcgtagcc gatggcgcaa cccagcgcgc aggcggccag gccgagcagc aggaattccg
     3061 cggcgtaaag cgaaagcagc tggcgctgcg tggcgccgaa gcagcgcatc accgcgcagc
     3121 cgtccaggtg gcgctccacg tagcggcgcg tgccgagcgc gatggcgacg cccgccagga
     3181 tcgccgccag gagcgcggtc aggccgagga agcgctgcgc gcggtcgatc gaggcgcgca
     3241 cctcgggccg cccgctttcc aggttgtcca cgcgctggcc gcgctcgagc cgcgcgcgcg
     3301 cccaggcgtc gaacgcggcg accgccgccg gctcgcccgc cgcatacagg tagaaccaga
     3361 cgcggctgcc ggtctgcacc agtccggtgg cgggcacatc gtcctggtgc atgaccaggc
     3421 gcggcgcaat gttgaagaaa ttggtgccgc gctcgggctc cagcgtgagg atcgccgcca
     3481 ccttgaactc cgcggtgccc agcctcaggc tcgcgcccat cggcaggccg agcgccgtca
     3541 ccaggcgctc ttccagccac accgtgccgc gcgcggggcc ctgctgcgcc ggcgcgtccg
     3601 gcgcgtttgg cgccggcgcg atgcgcagcc tgccgcgcag cggatagttc tgcgacaccg
     3661 ccttgacgcc ggcaaggagg ctcgattcgc ccgcgcgcgc catgctgatg aaattgacgg
     3721 cctcggcgcg ctgcaagccg cggcgcgcga tctcgtcggc catctcgcgt ttccaccgat
     3781 ggtcggacac cagcaccagg tcggcgccga ggagttggtg cgcatcgcgc accagggcgc
     3841 ggctcacgcg gtcggcgaag aaagccacgc tggtgatgct cgccaccgcc accaccagcg
     3901 ccgcggcgag cacgcgcacc tcgcccgcgc gccagtcccg cgcgagcatg cgcagcgcct
     3961 gccgcatcac gcgacgatcc tcccggcggc caggcgcacg atgcgcgtgc agcgctgcgc
     4021 caggtgctcg tcgtgcgtca ccagcaccag cgtggtgccg gcctcgcggt tcatctggaa
     4081 caggagcgcg atcaccgcgt cgccggcttc ggcatccagg ctgccggtgg gctcgtccgc
     4141 caggagcagc ttgggccgca cggcgaacgc acgcgccagc gccacgcgct gctgctcgcc
     4201 gcccgacagg tgcttcgggt agtggtgcgt gcgctcggcc aggccgacgc gcgccaggat
     4261 ctcgcgcgcg gcggcctcgg cgtcatcgcg gccggcgagc tcgagcggca gcatcacgtt
     4321 ctccagcgcg gtgagcgagg gcagcagctg gaaggtctgg aacacgaagc cgaccgcgcg
     4381 gccccgcagc tcggcgcgcg cgtcctcgtc cagctcgaac aggctggcgc cgtcgagttc
     4441 gaccgatccc gtgctgggcg tgtccaggcc ggcgagcagc gcgagcagcg tggatttgcc
     4501 cgagcccgag gcgccgacga tggccaccgc ctcgcccggc atgacctcga gcccgatatc
     4561 gcgcagaatg acgagatcgc gatccccgct cttcaccgtc ttgccgatgc cgcgcgccgc
     4621 cagaacgttg ctcgtctgcc tgttcatctg cttttcttcc ggggcctggg ggaaaacgat
     4681 cctcgtgtac ggcgacagcc tgtcggcggc ctacggcatc gccgagaagc gcggctgggt
     4741 ggcgctgctc ggcgaacggc tgcggcgcga gcggcccgat tatagcgtcg cgaacgcgag
     4801 catttcgggg gaaacctcga gcggcggcct cggccgcatg gccagggcgc tcgcgcagca
     4861 caagcccgcg gtgctggtgc tggagctggg cgccaacgac ggcctgcgcg ggctgccggt
     4921 ggcgcagatg aagcagaacc tgtcggcgat gatcctgcag gcgcagaaag ccggcgcgcg
     4981 cgtggtgctg gtcggcatgc gcatcccgcc caactacggc ccggagtaca cgcgcgaatt
     5041 cgaagcggcg ttcgccgatc tggcgcagcg gcaccgcacg gcgcgggtgc ctttcctgtt
     5101 cgccggcatg ggcgaggatc tcgccctgtt ccagccggac cgcatccacc cgaccgaagc
     5161 cgcgcagccg atcctgctgg acaacgtctg gaaggccctc cggcccctgc tcaagtgatc
     5221 gtcaggcgcg gcgcgccgac gacgaacttc ccgatgcggc gcgcctcggc gaaaccgcgc
     5281 ctggcgaact ccgccagcac agcctgctcc gcctgcggcg cgcaagccac cagcagcccg
     5341 ccgctggtct gcgggtcgga gacgagcttg cgcatccatt cgggcgcgcc ggccggcaat
     5401 tgcacctcgt cgccgtagcc ctgccagttg cggtccgaag cgcccgtcgc gacgccctgt
     5461 ttggcccact cgagcgcctc gccgatcacc ggcagcgact cgaacgccac ttgcgccgcg
     5521 agcttcgagc cgcgcaggat ttccagcagg tgccccgcca gcccgaaccc ggtgacgtcg
     5581 gtcagcgcat gcaccccggg catggcggcg agcgcggtgc cgggcgtgtt gagctgggtg
     5641 gtcgcggcga tcatggcgcg gtagccggcc tcggagagct tgcccttctt cagcgccgcc
     5701 gacaggatgc ccacgcccag cggctttccc agtatcagca cgtcgccggc ctgcgcggtg
     5761 ctgttgcgct tcacctgctc cgggtgcacc agtcccagcc ccaccagccc gtagatcggc
     5821 tccagcgtgt cgatcgagtg cccgccggcg atcgggatgc cggccgccgc acacaccgac
     5881 tcgccgccct cgagaatctt ctggatcgcc gagaccggca gcttgtccag cggcatgccg
     5941 acgatggcaa gcgccatgat cggcttgccg cccatggcgt agatgtcgga gagcgcattg
     6001 gtggccgcga tgcggccgaa gtcgtaaggg tcgtccacga tcggcgtgaa gaagtccgtg
     6061 gtggcgacca gcgcctgagt gtcgctcagg cggtacaccg cagcgtcgtc cgaggtctcg
     6121 gtgcccacca gcaagtccgc gggcaaaccg cggatcggcg tcgaggccag gatctcggac
     6181 aggacggcgg gcgcgatctt gcagccgcag ccgccgccgt ggctgaactg ggtgaggcgg
     6241 attctgtcgg gggcattcat aggcgtagaa ttttagtcga tgcagacggc attgcactcg
     6301 gttccgatgc aggcgtcgtc cctgacggcg tttgacgcca tcatcgacgc gcgctcgccc
     6361 gcagaattcg ccgaggacca cctgccgggc gccgtatcgc tgccggtgct cgacgatgcg
     6421 gagcgggcgc gggtcggcac ggtctacaag caggtgtcgc ccttcgccgc caagaagctc
     6481 ggcgcggcgc tggtggcgag gaacatcggc cggcacctcg agggcgcgct cgccgaccgg
     6541 cccaagggct ggcggccgct cgtgtactgc tggcgcggcg gcaagcgcag cggcgcattc
     6601 gcgcacgtgt tgcgcgagat cggctgggac gcgcgcgcgc tggagggcgg gtacaaggcc
     6661 taccggcaat gggtcgtggc gcggctcgcc gaagtcccgg gcacgctgcg cttccgtgtg
     6721 gtgcacggcg tcaccggcag cggcaagagc cggctgctga aagccctggc gcaggcaggc
     6781 gcgcaggtcc tcgacctgga ggatctggcg gcccaccgcg gctcggtgct gggcaacctc
     6841 cccgaccgcg cgcaaccgtc gcagaagatg ttcgacagcc gggtgctgca cgagctcgcc
     6901 gcctgcgatc cggggcgcgc ggtctacgtc gaaggcgagt cgaagaagat cggccagctg
     6961 caggtgcccg aggcgctgat ccaggccatg cgcgcctcgg attgcgtgct gctggaggcg
     7021 gacgtgcgca cgcgcgtcgc cctgctctcg gcggagtacc gtcatttctt caccgcgcgc
     7081 gaggcgctcg aggcgcggct cgattgcctg gtcgggctgc acgggcgcga gcgcgtgcag
     7141 agctggaagg cgctcgccgc gcagggcgca tggcaggagc tggtggcgcg cctgctggac
     7201 gagcactacg atccggcgta ccggcgctcg gcgcagcgca atttcacgcg cctgccgcag
     7261 gccagggtgt tgcgcgtgcc cgcggcggac gatgccgcct tcgccggcct ggcgcgcgag
     7321 ctggtcgcat gaggaccctg ctgctggcgc tcgcctgcgc ggccgcgccc gcggccgcgg
     7381 cgccggaact gcgtgcggcg ctcgagtccg gcgacctgat ctgcgaattc gaccacgaca
     7441 agcgcagcct gatcgccgac ctggccggcg gcgctcgtgc ctcggacctg ctgctggtat
     7501 acgagtcggt gcgcccggaa tcggcgcagg tcctgtcctc gagctcgccg ggccggcggc
     7561 cggtgctggt gcgagccacc gccagggccg tgcatttcat cgaacgggtg ggaccaagcg
     7621 tgcgcgtcac cacgctcacc cgctgcgagc tgcacaaatg gaagaacggg gcgcgggcct
     7681 gcgtccgcta cgccgcgcag cacgcctggc acttcgatgc gctcgccgcc gccgatccgg
     7741 acctcgcctt cgatcgcctg ccggcgggcg cctcgcccgg gcgctgcgaa ccctgggact
     7801 tggactgaca gtttcttgca ttcggcggga cgccagctag ctcatatgca gatggcatgc
     7861 ggcctttgct atagtggtcc gcaggatgga gatcaaggac ctgcgctatt tcatcgccgt
     7921 gtacgaagag ggcgggttcg gtcgcgccgg cgtgctgctg gaaaccgtgc aatccaacgt
     7981 gtccgcgcgc atccgccggc tcgagctgtt cctcggcgtg cggctgttcg agcgccgcca
     8041 ccgcggcgtg gtggtgacgg aaaaaggcga gaagctgtat cgccacgcca agcgcgtgat
     8101 cgcgctgctg gaggaaacct cggccgcggt aaaaggcaag gaagcggcct agcctaccgg
     8161 gccgcgcagg tacgaaagcc gcagaacatg tccgcgcgct ccggcgcgta gaagttgcga
     8221 aagcccgggc gcgcgagtcg cgccggggtc gaaaagctcg cgccgcgcag tacccgctgc
     8281 gtgccgaacc agggccgcga atagtcctcg tagggatcgg gcgagaatcc cggataaggc
     8341 aggaagtcgc tcgccgtcca ttcccatgcc tgcccggtgt gcaacgcgcc gcgcgtcgcg
     8401 gcgaattccc actccgcctc gctgggcagg cggcgcccgg cccaacggca gtaggcctgc
     8461 gcctccagag cgctcacgtg cagcacaggc tcctgcggcg cgagcgccag ccatcgctcg
     8521 aagcggcgct gctgccatgc ctgcccggct tttctccagt agcgcggcgc cgtgccgcca
     8581 gcctcgacgt aggccaggta ctcggcattg gtgacgagtt cggcggcgat gcgaaacggc
     8641 gccaactcga tttcgtgcgc ccacttctcg ttgtcgaacg cgaacgcccc gcccggctgc
     8701 acgcccagcg tgcatcgtcc acccggcagc ccgacgtccg cctgcggccc tgcccggatg
     8761 agcgcggagc cggccgggtc gccgtagccg agcgtctggt gcgtgtagtg gaaggcctcg
     8821 gcgtgcatgt cctcgtgaaa tacgcagagc tgcgcgtaat accgcaacgc cgcgttctcc
     8881 ggctcgcgcg cgagtcggtc cagcacccgc tcgagcacac cggacaggta ggcccgcgtg
     8941 gcggcaagcg tcggcagggg caggtcccag cgccggtcgt gcgccaccgc ggaagagtcg
     9001 tacagcgcgt cggcctcggg caggatcgac gccaccgccc cgccgcgcac ggtgcgcagg
     9061 caccagcgct cctggaacca gccgacatgg ccgatctccc acaggggcgg attgacgatg
     9121 gcgagcctgg gcccgagcag gcgctcgccg cccaggtcgc ccgtcatcag gtcggttcgt
     9181 gcgcgcgctt cgagcagctg gcgttcgagc atcgcgtgct agtctaacca acagccatgg
     9241 cgcgtatcgc gatcgtcacg ccggccggcg cggggacgcg caacggcaac cgccacaccg
     9301 cgctgcgctg ggccgcgcaa ttgcgtagcg cggggcacgg cgtatcggtg gatgtcgaat
     9361 ggagcggaaa atcctgcgcg ctcctcgtcg cgctgcacgc gcgccgcagc catggctcga
     9421 tcgcgcgcta tcgggaagcc catccgcacg gccccttgtg cgtagcgctg accggcacgg
     9481 atctgtaccg cgacttgccg gcgtcgcgcg aagcgcggca ctccctcgaa ctggccgacc
     9541 gcatcctcgt gctgcaggca gaggcgctgc ggcggcttgc gccggcgcag cgccgcaagg
     9601 cgcgcgtggt ctatcagtcc gccgattccg gtgcgcggcg cgcgccgccc gagcgcgtgt
     9661 ttcgcgtcgc ggtcatcggc cacctgcgcg aggagaagga tcctttccgc gcggcagccg
     9721 cgcttgccga gctgcccgcc gccgccggca tcgaagtggt ccagctgggc gcggcgctct
     9781 cgccggacat ggcgcgcgaa gcgcgccggc gcatggccgc ggagccccgc tatcgctggc
     9841 tcggcggcgt atcgcacgcg cgtgcgctcg gctggctggc gcgcagccag ctgctggtcg
     9901 tcagttcgcg catggagggc ggcgccaacg tgatcgcgga agccgcgcgc gcaggcaccc
     9961 cggtgctcgc ctcccgcgtg gacggcaaca tcggcatgct gggtcatgcg tacccgggat
    10021 acttccgcct cggtgaccag cacgcgctgg cgcgcctcat cctgcgcgcg gcaacggacc
    10081 cgcgctttta cctggccctg cgcaaggcgc tggccgcgcg gcgagcgcgc ttcgccccgg
    10141 ccgcggagcg cgccgcgctg ctgcgcgccc ttgccccgct gctcagaccg cgcgcgcggt
    10201 gatggcgtac ttgacgctgt ccgggtcgag ctggtcgcgc atcatgccgc cggtctgcgc
    10261 gttgatgtac atcaagtagg acagcttcgg cccgctggcc cccggcaggg cgatgtcgtg
    10321 gcccatgttg taggcaagcc agttcatctc ccaagcgccg aacaggcgct tgcgcgccgc
    10381 cgccaccttg gcatcgcccg ccggcaggtt gcccggcggc tcctccagga tcaccttgcg
    10441 cacgtcggcc gggtccaccg gcacccagcc ataccccttg gcgaagaact ccgcgcggca
    10501 gtgctgcgcg cgtgtcacgt tgggcgagcc cacccccagg ctccggtagc cgtacatcga
    10561 cggcgcgacg cgcaatccat agatgtcgcg cgccggcagg ccggcggcgc gcgcgagccc
    10621 gacgaacatc gcgttcaggt cgccgcactt gccgccgagg ttgccgcttt ccagcatgct
    10681 cttgatgtcg ccccagccgc agccccgcgt cttggggtcg cggaaggtgt tgtccaccac
    10741 ccactcgtag atcgcgcgcg ccttgtcgac gtcgccgcgc gcgccctggg tgacttgctg
    10801 cgcggtctgc ttcacgatgc cgtcggtggg gatgagttcc gtcggcgcgg tatagaacgc
    10861 ctgctcctcg gccgacaggc gacgcgggtc tgcgccgggg cgcgacaggt cgacgctgcg
    10921 ctcgcgcgtc atgaagcggc tcgtgacctc gatcaccggc tccgccacgc cgggggacca
    10981 ttcggcgacc agcatgccga cgccgtactt gccgtcgctc atcacgcgcg cttgcgccgc
    11041 gttgccgctc caggcgttgc ccacggcctt gtgccagggg gtgtcgtcgc tcaggggcag
    11101 cggcacccag gtgcgcgaca cgccgctcgg cttgaggatt tcgacgcgac tgacgatctc
    11161 ataggcctgc cagccctcgg ccgccagcgc cgcgcgccag ggcagcgccg aggcagtcgc
    11221 cgcggcgccg gcgcttttca ggaatgatct gcgatccatg ccaacctcct gtcttgtcaa
    11281 taggaacgac gccggcgtgg ccggcggaac tgcttgatct ggggagcggg agcggaagcg
    11341 gcaggaatgc ggaagggaag caggcaaatc gtcgcgaccc tgtcatccgg gcatcccgca
    11401 cgccctcgcc gcccgccgct ttgccgcgcg aatccgatca tcgccgccaa cgatcggcaa
    11461 ctggtcgaaa tcaggcgggc gagaagaaac atgctcggga gattctacgc ccagggaagg
    11521 cggctagcga tccaggctgg ccatcacccg caggtgctgc atcacgctgc gccccagggc
    11581 cgacagcgca taaccgcctt cgagcatcga caccatgcgc ccctgcgcgt gcctgtcggc
    11641 caccgccttc acctggcgcg tcacccagcc gtagtcggca tccgacaggc gcagcatggc
    11701 catgtcgtcc tcctcgtgcg cgtcgaagcc tgcggaaaac agcaccagct gcggtctgaa
    11761 ggcgtccagc gccgggatcc aggcctcgct gacggccgtg cggaactgcc tggacccggc
    11821 gcccgcggcg agcggcacgt tcaccatgtt gggcgcaggc gcctcggtgc cgctgtacgg
    11881 atagaacggg tgctggaagg tggacaccat cagcacctgc gcgtcgttct cgaagatctc
    11941 ttcggtgccg ttgccgtggt gcacgtcgaa atcgacgatc gccacgcgct gcaggccgtg
    12001 cgccgcgagc gcatggcgcg cggccaccgc gacattgttg aagatgcaga aacccatcgg
    12061 ccgcgcgcgg caggcatggt gtcccggcgg acgcacgctg caaaaggcgt tgtcggccgc
    12121 gccgcgcagc accaggtcgg tggcgagcac cgcggcgcca gccgcgcgca gcgccgcgtg
    12181 caggctgtgg ggattcatcg ccgtgtccgg gtcgagatgc accctgccgg actcgggcgc
    12241 cgcgtcgcgg atggcgcgca ggtactccgg cggatgcacg cgctccagct cctcgtccgt
    12301 ggcgagcggg gcctcgtggc gcgccagttg cgcgccgacc ccggaggcga tcagctggtc
    12361 ctcgatcgcc gtcaggcgct ccggccgctc cggatgcccg gcgcccatct cgtgcttcag
    12421 gcaatcggca tgcgtgatga aggcggtcgg catgatgcgc gaaatcatac cctgtacaat
    12481 cgggccatgc tcctgaaaac acagatcaaa acgcagcccg cgccgacgcc ggtcgagagc
    12541 ttggtcgcca ctgccggccg ggtgatcctc ggcaaggaaa cgcaggtgcg tctcgcgctc
    12601 gcctgcctga tcgcgcgcgg ccacctgctg atcgaggacc tgcccggcgt gggcaagacc
    12661 acgctggcgc acgtgctggc gcgcaccctc ggcctgcatt tccagcgcat ccagttcacc
    12721 agcgacatgc tgccggcgga catcatcggc gtgtcggtgt acgagcgcga cggcggcacg
    12781 ttcaagttcc accccgggcc gatcttcgcc caggtgatcc tcgccgacga ggtgaaccgc
    12841 gccacgccga agacgcagag tgccctgctc gaggcgatgg aggagcacca ggtgacggtg
    12901 gaaaccgaga ccaggaagct gcccgagccg ttcttcgtca tcgccacgca gaacccgtcg
    12961 gagcaggtgg gcaccttccc gctgcccgaa tcgcagctcg accgcttcac catgcgcatc
    13021 gagctgggct acccggatcg cgacgccgag cgcgcactgc tgacgggcac cgaccgccgc
    13081 gacctgctgg cgacgctgga gccctgcatg agcccggccg aactggtgga gctgcaggga
    13141 acggcgcagc gcgtgcacgt gtccgcggcg ctgctcgact acgtgcaggc gatcgtcgcg
    13201 cacacgcggc gctcgcctga ttacgtcgcc ggactgtcgc cgcgcgccgc gctggcgctg
    13261 gtgcatgcgg cgcgcgcctg ggccctgctg gagggccgcg acaaggtgat tccggaagac
    13321 gtgcaggcca tcctgccggg cgtcgcggcg caccgcctgc gcccggcgca cgacgccgcg
    13381 cgccgcgtgg acgtcggcgc gcagctgctc gccgcggtgc cgatccccta ggctgccgcc
    13441 cgttctgcgc ggcggcgctc gttttcccgc aggaagcgtt tgcgcaggcg gatcgatttc
    13501 ggcgtgattt ccaccagctc gtcgtcggcg atgaattcca ccgcgtattc cagcgtcagg
    13561 ccgatcggcg gcaccaggtc gatggcctcg tccttgcccg aggcgcggat gttggtgagc
    13621 tgcttggtgc ggatcggatt gaccaccagg tcgttgtcgc ggctgtggat gccgacgatc
    13681 atgccttcgt acaccggctc gccgggcgag acgaacatgc ggccgcgctc ctgcagcttc
    13741 cagatcgcgt aggccaccgc cacgccgtcg tcctgcgaca ccagcacgcc gttccggcgc
    13801 tcggccatgt cctcgggcct ggcgggggcg tattcgtcga acacgtggct catgattccg
    13861 gtgccgcgcg tcagggtcat gaattcgccc tggaagccga tcagcccgcg cgccgggatg
    13921 cggtaatcca ggcgcacgcg gcccttgccg tccggatgca tctcgagcag ctcgccgcgg
    13981 cgccggccca gctcctccat cacgccgccc tggttgacct cttccacgtc caccgtgagc
    14041 acctcgaagg gttcgctgcg cacgccgccg atctccttgt agaccacgcg cggccgcgac
    14101 acggcgatct cgtagccttc gcggcgcatg ttctccagca ggatcgtcag gtgcagctcg
    14161 ccgcggccgt gcacgatgaa ggtatcggtg tcgtccgggt actcgacgcg catcgccacg
    14221 ttgctcttgg tctcgcgctc caggcgctcg cggatctggc ggctggtgac gtacttgccc
    14281 tcgcgcccgg caagcggcga cgcgttgacc aggaaattca tggtcagcgt gggctcgtc
//
LOCUS       DASRIE010000082         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1898810, whole genome shotgun sequence.
ACCESSION   DASRIE010000082 DASRIE010000000
VERSION     DASRIE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898810"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFB53_03825"
     CDS             complement(<1..284)
                     /locus_tag="VFB53_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZN85895.1"
                     /translation="MNVEQARFNMIEQQIRPWDVLDQGVLSLLAVVKREDFVPAQWQA
                     LAFVDTEVPLPEGQCMLAPKVEARLLQELRVQRHERVLEVGAGSGYMAALL"
     gene            complement(360..1037)
                     /locus_tag="VFB53_03830"
     CDS             complement(360..1037)
                     /locus_tag="VFB53_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85896.1"
                     /translation="MSAKTAAPSRQRRKQARPQELLSAALELFVEKGFAATRSEEVAA
                     RAGVAKGTLYLYYPSKEDLLKAVVRENLSALIAEGASLAGRFEGSTHDLLVLLMQTWW
                     ERVGSTPASGIFKIIITEMGNFPDFARFYVQEVIEPGQALFTRVLERGIASGELRPVD
                     VFQAVHVLIFPMLMMCLHKHSLGACAGMRPLIDPPSFIRTHVDVVVHGLLARPAPAPP
                     RRARADA"
     gene            1239..>2337
                     /locus_tag="VFB53_03835"
     CDS             1239..>2337
                     /locus_tag="VFB53_03835"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN85897.1"
                     /translation="MTRPLRLALAFLVLALAGAAAWWFTRGSDGAVQYRTARIERGPL
                     QATVAASGTVTPVTQVQVGTQVSGQIQELFVDFNSEVKQGQLIARLDPESFQYKLRQV
                     QADLDASRASVMNAQANALAAQAAVSRANVDLAEARRALQRNEELVAQGFISPAQLDT
                     ARALVESQQAALRGAQAQAQVAQAQIASAQAAVKQREALVAQAKIDVERTEIRSPVDG
                     VVIKRSVELGQTVAASLQAPELFVIARNLSDMQVQVAIDEADISRVRLNQKATFTVDA
                     FAARTFDGTVTQVRKAATNTQNVITYTVVISFGNADARLLPGMTANVRLVTDTRENTL
                     KVPNAALRVRLPGVEPAAQAASRRRPADAQAR"
BASE COUNT          364 a          818 c          834 g          321 t
ORIGIN      
        1 agcagcgcgg ccatgtagcc cgagccggca ccgacctcca gcaccctttc gtggcgctgc
       61 acgcgcagtt cctgcagcag ccgggcttcg accttcggtg ccagcatgca ctggccttcg
      121 ggcaagggca cttccgtatc gacaaaggcc agggcctgcc attgcgccgg cacgaagtcc
      181 tcgcgcttga ccaccgccag caacgacagc acgccctggt cgagcacgtc ccaggggcgg
      241 atctgctgct cgatcatgtt gaagcgggct tgttcgacgt tcatggcggt gtttcggaca
      301 gtggattgga gggtggaggc aaagcctcga attctagggg gcgcgggctt cgggccgcct
      361 tacgcgtccg cgcgggcgcg ccgcggcggc gcgggcgcgg ggcgcgcgag caggccgtgc
      421 acgaccacgt ccacgtgcgt gcggatgaag gacggcggat cgatcagcgg ccgcatgccc
      481 gcgcacgcgc cgagcgaatg cttgtgcagg cacatcatga gcatcgggaa gatgagcacg
      541 tgcacggcct ggaacacgtc caccgggcgc agctcgccgc ttgcgatgcc gcgctccagc
      601 acgcgcgtga acagcgcctg gcccggctcg atcacctcct gcacgtagaa gcgcgcgaag
      661 tcggggaagt tgcccatctc cgtgatgatg atcttgaaga tgcccgacgc cggcgtgctg
      721 cccacgcgct cccaccacgt ctgcatgagc agcaccagca ggtcgtgcgt ggagccctcg
      781 aagcgcccgg cgaggctcgc gccctcggcg atgagggcgg acaggttctc gcgcaccacc
      841 gccttcagca ggtcttcctt gctggggtag tacaggtaca gcgtgccctt ggcgacaccc
      901 gcgcgcgcgg cgacctcctc cgagcgcgtg gcggcgaagc ccttctcgac gaaaagctcc
      961 agcgccgcgg aaagcagctc ctgcgggcgg gcttgcttgc ggcgctggcg cgagggcgcg
     1021 gcggtcttgg cggacatctc gaattactga ctgattggtc attaaatgta gccggtgcag
     1081 gctgcgacgg tcaacccgcg cagcgcccgc acccggggcg ccgccacgca ccgagtctgc
     1141 gcgacgcccg gtatcgcccc tttgcgatgg ggtaaagatg ggtgaagtga cggcaccgcg
     1201 ggccttgagg gcatgcgccg cgccgcaaaa tttggccgat gacccgaccg ctgcgcctcg
     1261 cccttgcctt cctggtcctc gcgctcgccg gtgcggcggc ctggtggttc acccgcggca
     1321 gcgacggcgc cgtgcagtac cgcacggcgc gcatcgagcg cggcccgctg caggcgaccg
     1381 tggccgcgtc cggcaccgtg acgcccgtca cgcaggtgca ggtcggcacg caggtgtccg
     1441 gccagatcca ggagctgttc gtcgacttca actccgaggt gaagcagggc cagctcatcg
     1501 cgcgcctcga ccccgagtcg ttccagtaca agctgcgcca ggtgcaggcc gacctcgacg
     1561 cgtcgcgcgc gtcggtgatg aacgcgcagg ccaacgcgct cgccgcgcag gccgccgtgt
     1621 cgcgcgccaa cgtggacctg gccgaggcgc gtcgcgcgct gcagcgcaac gaggagctgg
     1681 tggcgcaggg cttcatctcg ccggcgcagc tcgacacggc gcgcgccctg gtcgagtcgc
     1741 agcaggcggc gctgcgcggc gcgcaggcgc aggcgcaggt ggcgcaagcg cagatcgcga
     1801 gcgcgcaggc ggcggtgaag cagcgcgagg ccctggtcgc gcaggccaag atcgacgtcg
     1861 agcgcaccga gatccgctcg ccggtggacg gcgtggtgat caagcgcagc gtcgagctgg
     1921 gccagacggt ggccgcgagc ctgcaggcgc ccgagctgtt cgtaatcgcg cgcaacctga
     1981 gcgacatgca ggtgcaggtg gcgatcgacg aggccgacat cagccgcgtg cggctgaacc
     2041 agaaggccac cttcaccgtc gacgccttcg ccgcccgcac cttcgacggc acggtgacgc
     2101 aggtgcgcaa ggccgcgacc aacacgcaga acgtcatcac ctacaccgtg gtgatctcct
     2161 tcggcaacgc ggacgcccgg ctgctgcccg gcatgaccgc caacgtgcgg ctcgtgacgg
     2221 acacccgcga gaacacgctg aaggtgccca acgccgcgct gcgcgtgcgc ctgccgggcg
     2281 tcgagcccgc ggcgcaggcg gcgtcgcggc ggcggccggc cgatgcgcag gcgcgcg
//
LOCUS       DASRIE010000083         9546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3206051, whole genome shotgun sequence.
ACCESSION   DASRIE010000083 DASRIE010000000
VERSION     DASRIE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206051"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFB53_03840"
     CDS             complement(<1..844)
                     /locus_tag="VFB53_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN85898.1"
                     /translation="MASVPLVAGAAGSAPLAWRAGRPISAAQYLADAAQLAALLPERR
                     HVLNICTDRYRFAVGFGAALLRRQVTLLPPNQTPETLRWLAQFAPDAYALADEAQPGL
                     DLPRVAFPELGGPARADPPVPAIDADQLCAIVFTSGSTGSPVPNRKHWGFLTRNVRGA
                     IERWGAAAAPRSILGTVPPQHMYGFESTVLAALQGGGALCAERPFYPDDLCAALERLP
                     RPRVLVSTPFHLRSLLEDVAQPPPADLVLCATAPLDAPLAQRAEAAFGATLLEIYGST
                     ETGQM"
     gene            complement(871..1539)
                     /locus_tag="VFB53_03845"
     CDS             complement(871..1539)
                     /locus_tag="VFB53_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85899.1"
                     /translation="MNPARTGRWLALLIALALGYALLAHLFTTAAQPTALGAAASTAP
                     WALLALAVAWRSAHRVAALLACALLVGLGWFYWEQLERNTPWVFYLQHVIMFVALGAA
                     FGVTLRPGQEALCTRLARAVQGPLSAGAEAYSRGVTLAWALFCAVLVAVSTLLFFLGP
                     LAAWSVFANLLTLPLVGAMFLVEALVRARLCPELSQGGALRGAATSVKAYWESSVRPT
                     PGPR"
     gene            complement(1536..1835)
                     /locus_tag="VFB53_03850"
     CDS             complement(1536..1835)
                     /locus_tag="VFB53_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZN85900.1"
                     /translation="MASIPLSAQFAPSADPALLQELAGLIAAELNLDVQPADIAPEAP
                     LYGEGLGLDSIDILELALVVSRRYGVRLRSDHEDNAVIFGSLSNLARFIAQNRTK"
     gene            complement(1878..3137)
                     /locus_tag="VFB53_03855"
     CDS             complement(1878..3137)
                     /locus_tag="VFB53_03855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZN85901.1"
                     /translation="MSDRRVAVTGLGVLSPVSPGDAAAGAFFDALLAGRSGLRLYAPP
                     DAPRPFAVPAGVCAGFDADALLGKARASTMDRYSQLGVAAGLAAWRDAGLAAARDGDR
                     DDWGVAWGTSLGGALTFERGYRDAFVAGRDRVAPLTVVQAMTNACASHLAIGFGLGGA
                     CSTYSVACSSAAIAIGEAMRRVRSGAAPVMLAGGSDAPLAFGVIRAWLALQVLAPGDE
                     GSAPQSCRPFQSARAGLVLAEGAAALVLEDWERAERRGARIYAELAGYGESCDRSNLV
                     RPDRDGQGRALRAALSDAGLAPEELDYVNAHATGTREGDTVEVAALVAALGAHAPRVA
                     VSATKSAHGHPLGAAGAIEAVATVLTLHRGSIPPTLGDPAGLDPDCAGLEHVIGSARG
                     GRGLRAALSNSFAFGGSNAVLAFRAAA"
     gene            complement(3134..3370)
                     /locus_tag="VFB53_03860"
     CDS             complement(3134..3370)
                     /locus_tag="VFB53_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN85902.1"
                     /translation="MQDTAPVFRFVEERLGIDLQAADAGAELAGLGVDSLSLLELMFE
                     IEEKFGVSIDEDTPPPKNCGELSALIERIAAAKP"
     gene            complement(3375..4340)
                     /locus_tag="VFB53_03865"
     CDS             complement(3375..4340)
                     /locus_tag="VFB53_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85903.1"
                     /translation="MRKTAAALLALCAFGAQAQEYPGKPVTMIVPFPPGGVADIVGRP
                     LAAAMEKSLGQPVVVVNRTGAGGAIGMTAAAKAAPDGYTILMALSSISIFPVSDPMQG
                     NPAPYQISDFMPIALVTADPTVLVVSAASPYKTIQDFVAAAKANPGKINYSSSGIFGT
                     LHVAMEMFANAAGIQLFHVPYQGGGPAVTALLGGQVQALASGPAAAVGQIKGGKMRAL
                     AGWGAKRLDLMPELPTFKELGYQDVEFYIWSAVVAPAAIPANVQHRLRAAVRAAVADP
                     QFTGAMAKVQTPISYLDAPEFKDFWVADANRLAAAVRKIGKSAAK"
     gene            complement(4337..5137)
                     /gene="fabI"
                     /locus_tag="VFB53_03870"
     CDS             complement(4337..5137)
                     /gene="fabI"
                     /locus_tag="VFB53_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZN85904.1"
                     /translation="MPLLEGRRILVTGLLSNRSIAYGVAKAARREGAALAFTYQNERF
                     KERVAEMARELGSDIALPCDVAEDAQIAALFGELGRRWDGLDGLVHAIGFAPREAIAG
                     DFLDGLSRESFRIAHDVSAYSFPALAKAALPLMQGRKAALVTLSYLGAERVVPNYNTM
                     GLAKASLEASVRYLAASLGPRGIRVNGISAGPIKTLAASGIAGFGRILKFVEEHAPMR
                     RNVTTEDVGNAAAFLLSELAAGVTGEVLYVDSGFRNVVAGIPGGVSET"
     gene            5161..7233
                     /locus_tag="VFB53_03875"
     CDS             5161..7233
                     /locus_tag="VFB53_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN85905.1"
                     /translation="MRPILLLAVLAAGCADVWNDPYPAADRGGNILYSVFTERPKHLD
                     PVQSYTEDEQRFIQQVYEPPLQYHYLKRPYELAPLTALEVPRPVEVQGGKYTVYTIRI
                     RPGIRYQPHPAFVPENHGLAPARVARLASPYDLPLGTRELTADDYIYEIKRLASPRLH
                     SPVLGLMAGYIVGLQEFADRLKKFDTKKGEWLDLRKHRLEGVEKVDAHTYRIRIKGRY
                     PQFAYWLAMPFFAPVPWEAERFFHQPGMAERNFTLDWWPLGTGPYMLTQNDPNARMLL
                     ERNPHFRGEPYPAEGEPGDAKEGLLADAGQAMPFVERVVFTREKENVPFWNKFLQGYY
                     DTSGIPSETFDQAVRVAVGGEVGLTEEMAAKGIRLRTSVDTSILYLGVNWLDAVVGGD
                     SERSRKLRQALAIAVDWEEYISIFLNGRGLVAQDPLAPGIFGQPEGRAAVNPQTHLWR
                     DGAARRRPIEDARKLLAEAGYPDGRHAKTGEPLVLYLDTVGRGPGDKPRFDWWRRQFD
                     KISVQLEIRDTDWNRFQEKIRKGSQQLYVLGWNADYPDPENFLFLLHGPQSRMKTQGE
                     NTSNYVNAEFDALFERMKNVPNSPERLKIIHRMVDIARRDLPWLGGFHPVAYGLEHPW
                     VMNGKPNNMARNNLKYLRVDPALRAAKRHEANRPVVWPAVLLFALLAAGSAPAVLSYR
                     RRERMAAR"
     gene            7230..8207
                     /locus_tag="VFB53_03880"
     CDS             7230..8207
                     /locus_tag="VFB53_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85906.1"
                     /translation="MTAYIIRRTLYAIPILIGINLITFALFFLVNTPDDMARMQLGVK
                     RVTPEAIEKWKAERGYDKPLLWNGAAPGAHKATDTIFFEKSVKLFALEFGRAEDNRDI
                     GNEIRTRMWPSLALAIPVFLVGLAVNITFALGMAFFRATYLDFWGVILCVAMMSISSL
                     FYIIGGQYLISKLWHLVPISGYEGGPDAVKFLVLPVLVGVIGGIGSGSRWYRTLFLEE
                     MGRDYVRTARAKGLAESTVMFRHVLKNALIPILTGVVVVIPLLFMGSLIAESFFGIPG
                     LGSYTIDAIQAQDFAVVRSMVFIGSVLYILGLLLTDISYTFVDPRVRLN"
     gene            8209..>9546
                     /locus_tag="VFB53_03885"
     CDS             8209..>9546
                     /locus_tag="VFB53_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85907.1"
                     /translation="MPVLLATDLLVWLLAAAVAGFAWYSSRRPHLALPWARVFRSRAA
                     VASSVFLAVFIALGLLDSLHYRPQLPARDPAAAPAYAVEVRSLLDVLLTPLRTKLEKT
                     YSAPLATRLYQKEQVELPGGKTVREFPRLAHGGAHLKEESHWGRDVALRLLAGLAIAI
                     ALWSLAVLAARRTLVRRWPQVPWGAAAAALGALLALAGPAALLAAGYHVFGTDRVGSD
                     VLYQALKSIRTSLVIGTLTTLVLLPAGIAAGISAGYFKGWVDDFIQYLYTTLNSIPGV
                     LLIAASVLMVQVYVDTHPGLFPTAAQRADFRLLSLCVILGALGWTGLARLLRGETLKL
                     REVEYVQAARAFGVSDWRIMARHVAPNVMHIVLIAVVMDFSGLVLAEAVLSYVGVGVD
                     PSMISFGTMINNARLELAREPMVWWSLASAFVFMFLLVLCANLFSDAVRDAFDP"
BASE COUNT         1370 a         3376 c         3458 g         1342 t
ORIGIN      
        1 ccatctggcc ggtctcggtg ctgccgtaga tctcgagcag cgtggcgccg aaggccgcct
       61 cggcgcgctg cgccagcggc gcgtcgagcg gcgcggtggc gcacagcacc aggtcggcgg
      121 gcgggggctg cgccacgtcc tcgagcagcg agcgcaggtg gaagggcgtg ctcaccagca
      181 cgcgcggccg cggcaggcgc tcgagcgcgg cgcacaggtc gtcgggatag aacggccgtt
      241 ccgcgcacag cgcgccgccg ccctgcagcg cggcgagcac cgtggattcg aagccgtaca
      301 tgtgctgcgg cggcaccgtg ccgaggatcg agcgcggcgc ggcggcggcg ccccagcgct
      361 cgatggcgcc gcgcacgttg cgcgtgagga agccccagtg cttgcggttg ggcaccggcg
      421 agccggtcga gcccgaggtg aacacgatcg cgcacagctg gtcggcgtcg atcgcgggaa
      481 cgggcggatc cgccctcgcc gggccgccga gctccgggaa cgccacgcgc ggcaggtcga
      541 gccctggctg cgcctcgtcg gcgagcgcgt aggcgtccgg ggcgaactgc gccagccagc
      601 gcagggtctc gggcgtctgg ttcggcggca gcagcgtcac ctggcggcgc agcagcgccg
      661 cgccgaagcc gacggcgaag cggtagcggt cggtgcagat gttgagcacg tggcggcgct
      721 cgggcagcaa ggcggcgagc tgcgccgcgt cggccaggta ttgcgcggcg ctgatcgggc
      781 ggccggcgcg ccaggcgaga ggcgcgctcc ctgcggcgcc ggcgacgagc gggacgctgg
      841 ccatgctcgc ggcggcgcgg gcgccggcgc tcagcgcggg cctggcgtcg ggcgcacgct
      901 gctttcccag tacgccttca cgctggtcgc cgcgccgcgc agcgcgccgc cctggctgag
      961 ctcggggcag aggcgcgcgc gcaccagggc ttcgacgagg aacatggcgc cgaccagggg
     1021 cagggtcagg agattggcga ataccgacca ggcggccagc ggaccgagga agaacagcag
     1081 cgtcgagacg gcgaccagca ccgcgcagaa cagcgcccag gcgagggtca cgccgcgcga
     1141 gtaggcctcg gcgccggcgc tcagcggacc ctgcaccgcg cgcgccaggc gcgtgcacag
     1201 cgcttcctgc cccgggcgca gggtcacgcc gaacgccgcg cccagcgcga cgaacatgat
     1261 gacgtgctgc aggtaaaaca cccacggggt gttgcgttcg agctgttccc agtagaacca
     1321 gcccagcccg accagcagcg cgcaggcgag cagcgcggcc acgcggtgcg ccgagcgcca
     1381 ggcgaccgcc agcgcgagca gggcccaggg agcggtggag gccgcggcgc cgagcgcggt
     1441 gggctgcgct gcggtggtga acaggtgcgc gagcagcgca tagcccagcg cgagcgcgat
     1501 cagcagcgcg agccagcggc cggtgcgcgc gggattcact tcgtgcggtt ttgcgcgatg
     1561 aagcgggcga gattcgacag cgagccgaag atcaccgcgt tgtcctcgtg gtcggagcgc
     1621 aggcgcacgc cgtagcgccg ggacaccacc agcgccagct ccaggatgtc gatggaatcc
     1681 aggccgaggc cttcgccgta cagcggcgcc tccggcgcga tgtcggcggg ctgcacgtcc
     1741 aggttgagct cggcggcgat cagcccggcg agctcctgca gcagggcggg gtcggcgctg
     1801 ggcgcgaatt gcgcgctgag gggaatgctg gccatgattg cgtcccgcag tttagccgct
     1861 gcacccgccg cggacaatca cgccgcggcg cggaacgcca gcaccgcgtt gctgccgccg
     1921 aaagcgaagg aattggacag cgcggcgcgc agcccccgcc cgccgcgcgc gctgccgatg
     1981 acgtgctcga ggcccgcgca atccgggtcc aggccggccg ggtcgcccag cgtcggcgga
     2041 atgctgccgc ggtgcagggt gagcaccgtg gccaccgcct cgatggcgcc ggcggccccc
     2101 agcgggtggc cgtgcgccga cttggtcgcg ctcacggcga cgcggggggc gtgagcgccg
     2161 agcgccgcga cgagcgccgc gacctccacc gtgtcgcctt cgcgcgtgcc ggtggcatgg
     2221 gcgttgacgt agtccagctc ctccggcgcg aggccggcgt cggacagcgc ggcgcgcagc
     2281 gcccgcccct ggccgtcgcg atccgggcgc accaggttgc tgcggtcgca ggactcgccg
     2341 tagccggcga gctcggcata gatgcgcgct ccgcgccgct cggcgcgttc ccagtcctcc
     2401 agcaccagcg ccgcggcgcc ctcggcgagc accagcccgg cgcgcgcgct ctggaacggg
     2461 cggcaggact gcggcgccga gccctcgtct ccgggcgcca gcacctgcag ggcgagccag
     2521 gcgcggatca cgccgaaggc gagcggcgcg tccgagcccc cggcgagcat caccggcgcg
     2581 gcgcccgagc gcacgcgccg catcgcctcg ccgatggcga tggcggccga ggagcaggcc
     2641 accgaatagg tgctgcaggc cccgcccagg ccgaagccga tcgccaggtg cgaggcgcag
     2701 gcgttggtca tcgcctgcac cacggtgagc ggcgccacgc ggtcgcgccc ggcgacgaag
     2761 gcgtcgcggt agccgcgctc gaaggtgagc gcgccgccga gcgaggtgcc ccaggccacg
     2821 ccccagtcgt cccggtcgcc gtcgcgcgcc gcggcgaggc cggcgtcgcg ccaggcggcg
     2881 aggccggcgg cgacgccgag ctggctgtag cggtccatgg tgctggcgcg cgccttgccg
     2941 agcagggcgt cggcgtcgaa accggcgcac acgccggcgg gcacggcgaa aggccgcggc
     3001 gcatcgggcg gcgcgtacag ccgcagcccg gagcggccgg cgagcagcgc gtcgaagaag
     3061 gcgccggcgg cggcgtcccc cgggctcacc gggctcagca cgcccaggcc ggtgaccgcg
     3121 acgcggcgat cgctcacggc ttggcggccg cgatgcgctc gatcagcgcc gacagctcgc
     3181 cgcagttctt cggcggcggc gtgtcctcgt cgatcgacac gccgaacttt tcctcgatct
     3241 cgaacatcag ctcgagcagc gacagcgagt ccacgcccag gccggcgagc tcggcgccgg
     3301 catcggccgc ttgcaggtcg atgcccaggc gctcctcgac gaagcggaac acgggcgcgg
     3361 tgtcctgcac tcgattactt cgccgccgac ttgccgatct tgcgcaccgc cgccgcgagg
     3421 cggttcgcgt cggccaccca gaagtccttg aactccggcg cgtcgaggta gctgatgggc
     3481 gtttgcacct tggccatggc gccggtgaac tgcgggtccg cgacggcggc gcgcaccgcg
     3541 gcgcgcagcc tgtgctgcac gttcgccggg atggcggccg gcgccaccac cgccgaccag
     3601 atgtagaact cgacgtcctg gtagccgagc tccttgaagg tcggcagctc cggcatcagg
     3661 tcgaggcgct tggcgcccca gccggccagc gcgcgcatct tgccgccctt gatctgcccg
     3721 accgcggcgg ccgggccgga ggccagcgcc tgcacctggc cgccgagcag cgccgtgacc
     3781 gccggcccgc ccccctggta gggaacgtgg aacagctgga tccccgctgc gttggcgaac
     3841 atctccatcg ccacgtgcag cgtgccgaag atgccggagg aggagtagtt gatcttgccg
     3901 gggttggcct tggccgcggc gacgaagtcc tggatcgtct tgtacgggct ggcggcgctg
     3961 accaccagca ccgtcgggtc cgccgtcacc agcgcgatcg gcatgaagtc cgagatctgg
     4021 tagggcgcgg gattgccctg catgggatcg gacaccggga agatcgagat cgacgacagc
     4081 gccatcagga tggtgtagcc gtcgggcgcc gccttggccg ccgcggtcat gccgatggcg
     4141 ccgccggcgc ccgtgcggtt gaccaccacc accggctggc cgagggattt ctccatcgcc
     4201 gcggcgagcg gccggccgac gatgtcggcg accccgcccg gcgggaaggg cacgatcatg
     4261 gtgaccggct tgcccgggta ctcctgcgcc tgcgcgccga aagcgcacag ggccaaaagc
     4321 gcggcagctg tttttctcat gtctcgctca ctcctcctgg aatgcctgcg accacgttgc
     4381 gaaagccgga atccacgtac agtacctcgc cggtgacccc ggcggcgagt tccgacagca
     4441 ggaacgcggc ggcgttgccc acgtcctcgg tggtgacatt gcgccgcatc ggcgcgtgct
     4501 cctcgacgaa cttgaggatc ctgccgaagc ccgcgatgcc cgaggcggcg agcgtcttga
     4561 tgggtcccgc ggagatgccg ttgacgcgga tgccgcgcgg gccgaggctg gccgcgaggt
     4621 agcgcaccga ggcctccagg ctcgccttgg cgaggcccat ggtgttgtag ttgggcacca
     4681 cgcgctcggc gccgaggtag ctgagggtga ccagcgcggc cttgcgcccc tgcatcaggg
     4741 gcagcgccgc cttggccagc gcggggaagc tgtaggccga gacgtcgtgc gcgatgcgga
     4801 acgactcgcg cgacagcccg tcgaggaagt cgcccgcgat cgcctcgcgc ggcgcgaagc
     4861 cgatggcgtg caccaggccg tccaggccgt cccagcgcct gccgagctcg ccgaacagcg
     4921 ccgcgatctg ggcgtcctcg gcgacatcgc agggcagggc gatgtccgag cccagctccc
     4981 gcgccatctc cgcgacgcgc tccttgaagc gctcgttctg gtaggtgaag gcgagcgcgg
     5041 cgccttcgcg gcgcgccgcc ttggcgacgc cgtaggcgat ggagcggttg ctgagcaggc
     5101 cggtgaccag gatgcggcgg ccttcgagta gcggcatggt gcggcgatta tacttgcgcg
     5161 atgcgcccga tcctgctgct ggcggtgctc gccgccggct gcgccgacgt gtggaacgat
     5221 ccgtacccgg ccgcggatcg cggcgggaac atcctctact cggtgttcac cgagcgcccc
     5281 aagcacctcg acccggtgca gtcctacacc gaggacgagc agcgcttcat ccagcaggtc
     5341 tacgagccgc cgctgcagta ccactacctg aaacggccct acgagctggc gccgctcacc
     5401 gccctcgagg tgcccaggcc ggtggaggtg cagggcggca agtacaccgt ctacaccatc
     5461 cgcatccggc ccggcatccg ctaccagccg catccggcgt tcgtgccgga aaaccacggc
     5521 ctcgcgccgg cgcgcgtggc gcgcctggcg agcccctacg acctgccgct cggcacgcgc
     5581 gagctcaccg ccgacgatta catctacgag atcaagcgcc tcgccagccc gcgcctgcac
     5641 tcgccggtcc tcggcctgat ggccggctac atcgtcgggc tgcaggagtt cgccgaccgg
     5701 ctgaagaagt tcgacaccaa gaagggcgag tggctcgacc tgcgcaagca ccgcctggag
     5761 ggcgtcgaga aggtggacgc gcacacctac cgcatccgca tcaagggccg ctacccgcag
     5821 ttcgcctact ggctggcgat gccgttcttc gcgccggtgc cctgggaggc ggagaggttc
     5881 ttccaccagc cgggcatggc cgagaggaac ttcaccctcg actggtggcc gctgggcacc
     5941 ggcccctaca tgctgaccca gaacgacccc aatgcgcgca tgctcctcga gcgcaatccg
     6001 catttccgcg gcgagcccta tccggcggag ggcgagcccg gcgacgcgaa ggaggggctg
     6061 ctcgccgacg ccggccaggc gatgccgttc gtcgagcgcg tggtattcac gcgcgagaag
     6121 gagaacgttc ccttctggaa caagttcctg cagggctact acgacacttc cgggatcccc
     6181 agcgaaacct tcgaccaggc ggtgcgcgtg gcggtgggcg gcgaggtggg cctcaccgaa
     6241 gagatggcgg cgaagggcat ccggctgcgc acctcggtgg acacgagcat cctgtacctc
     6301 ggggtcaact ggctcgacgc ggtggtgggc ggggactcgg agcgctcgcg caagctgcgc
     6361 caggcgctcg ccatcgcggt ggactgggag gaatacatct ccatcttcct caacggccgc
     6421 gggctggtgg cgcaggaccc gctcgcgccg ggcatcttcg gccagcccga ggggcgcgcg
     6481 gcggtcaacc cgcagaccca cctctggcgc gacggcgccg cgcggcgccg gccgatcgag
     6541 gacgcacgaa aactccttgc cgaggctggg tacccggacg ggcgccacgc gaagaccggc
     6601 gagccgctgg tgctgtacct ggacaccgtc gggcgcggcc ccggcgacaa gccgcgcttc
     6661 gactggtggc ggcgccagtt cgacaagatc tcggtgcagc tggagatccg cgacaccgac
     6721 tggaaccgct tccaggaaaa gatcaggaag ggcagccagc agctctacgt gctgggctgg
     6781 aacgccgact acccggaccc ggagaacttc ctgttcctcc tgcacgggcc gcagtcgcgc
     6841 atgaagacgc agggcgagaa caccagcaac tacgtgaacg cggaattcga cgcgctgttc
     6901 gaacgcatga agaacgtgcc caattcgccc gagcgcctga agatcatcca tcgcatggtg
     6961 gacatcgcgc ggcgcgacct gccctggctg ggcggcttcc atccggtggc ctacggcctg
     7021 gagcatccct gggtgatgaa cggcaagccg aacaacatgg cgcgcaacaa cctgaagtac
     7081 ctgcgcgtgg accccgcact gcgcgccgcg aaacggcacg aggcgaaccg cccggtcgtg
     7141 tggccggcgg tgctgctctt cgccctgctg gcggcgggat cggcgccggc agtcctgagc
     7201 taccggcgcc gggagcggat ggcggcccga tgaccgccta catcatccgc cgcacgctgt
     7261 acgccatccc gatcctgatc ggcatcaacc tcatcacctt cgcgctgttc ttcctggtga
     7321 acacgccgga cgacatggcg cgcatgcagc tgggcgtgaa gcgcgtgacg cccgaggcga
     7381 tcgagaagtg gaaggccgag cgcggctacg acaagccgct gctgtggaac ggcgccgcgc
     7441 cgggggcgca caaggccacc gacaccatct tcttcgaaaa gtcggtgaag ctgttcgccc
     7501 tggaattcgg ccgcgccgag gacaaccgcg acatcggcaa cgagatccgc acgcgcatgt
     7561 ggccgagcct ggcgctcgcc atcccggtgt tcctggtcgg cctggcggtg aacatcactt
     7621 tcgcgctggg catggccttc ttccgcgcca cctacctcga tttctggggc gtgatcctgt
     7681 gcgtggcgat gatgtcgatc tcgtcgctgt tctacatcat cggcggccag tacctgatct
     7741 ccaagctctg gcacctggtg ccgatctcgg gctacgaggg cgggccggac gcggtgaagt
     7801 tcctggtgct gccggtgctg gtgggggtga tcggcggcat cggcagcggc agccgctggt
     7861 accgcacgct gttcctcgag gagatgggca gggactacgt gcgcaccgcg cgcgccaagg
     7921 gtctcgccga gtcgacggtg atgttccgcc acgtgctgaa gaacgccctg atcccgatcc
     7981 tcaccggggt ggtggtggtg attccgctcc tgttcatggg cagcctgatc gccgagtcct
     8041 tcttcggcat cccggggctg ggcagctaca ccatcgacgc catccaggcg caggatttcg
     8101 ccgtggtgcg ctcgatggtg ttcatcggct cggtgctcta catcctcggg ctgctgctca
     8161 ccgacatctc ctacaccttc gtcgatccgc gcgtgaggct caactagcgt gccggtcctc
     8221 ctcgccaccg atctcctggt ctggctgctg gcggccgccg tggcgggctt cgcctggtac
     8281 agcagccgca ggccgcacct cgccttgccc tgggcgcgcg tgttccgcag ccgcgcggca
     8341 gtcgcatcct cggtgttcct cgcggtgttc atcgccctcg gcctgctcga ctcgctgcac
     8401 taccggccgc agcttccggc cagggacccc gcggcggcgc cggcctatgc ggtcgaggtg
     8461 cgctcgctgc tcgatgtgct gctgacgccg ctgcgcacga agctggagaa gacctactcg
     8521 gcgccgctcg ccacgcgcct gtaccagaaa gagcaggtgg agctgcccgg ggggaagacg
     8581 gtgcgcgagt tccctcggct cgcgcacggc ggcgcgcacc tgaaggagga gtcccactgg
     8641 ggccgcgacg tggcgctgcg gctgctggcc ggcctcgcca tcgcgatcgc gctgtggtcg
     8701 ctcgccgtgc tcgccgcccg gcgcacgctg gtgcggcgct ggccgcaggt gccctggggc
     8761 gcggcggcgg ccgcgctcgg cgcgctgctg gccctcgccg gccccgcggc gctgctcgcc
     8821 gcgggctacc acgtgttcgg caccgaccgc gtgggctccg acgtgctgta ccaggccctg
     8881 aagagcatcc gcaccagcct ggtgatcggc acgctgacca cgctggtgct gctgccggcg
     8941 ggcatcgccg ccggcatctc ggccggctac ttcaagggct gggtggacga cttcatccag
     9001 tacctgtaca ccacgctcaa ctcgatcccc ggggtgctgc tgatcgccgc ctcggtgctg
     9061 atggtgcagg tgtacgtgga cacgcacccg ggcctgttcc ccaccgcggc gcagcgcgcc
     9121 gacttccgcc tgctgtcgct gtgcgtgatc ctcggcgcgc tcggctggac cggcctcgcg
     9181 cgcctgctgc gcggcgagac gctcaagctg cgtgaggtgg aatacgtgca ggccgcccgt
     9241 gcgttcggcg tttccgactg gcgcatcatg gcgcgccacg tggcgcccaa cgtcatgcac
     9301 atcgtgctga tcgcggtggt gatggacttc agcgggctgg tgctggccga ggcggtgttg
     9361 tcctacgtgg gcgtgggcgt ggacccctcg atgatcagct tcggcaccat gatcaacaac
     9421 gcgcgcctgg agctcgcgcg cgagccgatg gtgtggtggt cgctcgcgtc ggcgttcgtg
     9481 ttcatgttcc tgctcgtgct ctgcgccaat ctcttctccg atgcggtgcg cgacgcgttc
     9541 gacccg
//
LOCUS       DASRIE010000084         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1899931, whole genome shotgun sequence.
ACCESSION   DASRIE010000084 DASRIE010000000
VERSION     DASRIE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899931"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFB53_03890"
     CDS             complement(<1..272)
                     /locus_tag="VFB53_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85908.1"
                     /translation="MNAVKAIVGAIALATALSAQAQWKPTKPINIIVPWAAGGATDQV
                     TRVAAGELEDALGAKIVVINQPGGAGSIGTMNAMNAAKDGYTWTAGA"
     gene            complement(289..915)
                     /locus_tag="VFB53_03895"
     CDS             complement(289..915)
                     /locus_tag="VFB53_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN85909.1"
                     /translation="MARNGRRDPARNQDRILKAAMAEFARYGLGGARVDRIAARAGAN
                     KRMLYYYYGDKEALFLAVLEDSYARIRRAELGLHLLDLDPVQGVRRLVEFTWDYYLKH
                     PEFLTLLNSENLHRARHLKRSRDIAAMHSPLIALLRDLLRRGERTGKFRRGVDPVQLY
                     ISIAALGYFYLSNRHTLSTIFARDLLSSRNKSERLKHMTALVLGYLSK"
     gene            complement(921..1871)
                     /locus_tag="VFB53_03900"
     CDS             complement(921..1871)
                     /locus_tag="VFB53_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN85910.1"
                     /translation="MKRLALLFLALFASAAWAQYPSKPIRMLVGFPPGGSTDLAARAL
                     GDKLSQALGQPIVVENKAGASGNIAAEQVARAAPDGHTLYMAPTSFATAPAFFAKLSW
                     DPVKDFTPVSLVATVPIIAVTHPAVPARTVQQLVAYAKANPGRLNMASPGATTLTRLT
                     GERFKQIAGIDWVTVHYKGGAPAMQDMLAGTAQVMFANISDVLTQVKAGRLNAIAVTT
                     PQRSPAVPEVPTFAESGYPEYAFMTWQAVVGPAGLPQEVVQRLNGEIVRIMATPEIKE
                     RFASFGTDAASSTPEELGRFLAAEVAKIAAVARSAGVKAE"
     gene            complement(1892..3367)
                     /locus_tag="VFB53_03905"
     CDS             complement(1892..3367)
                     /locus_tag="VFB53_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85911.1"
                     /translation="MKHRVLFGDIHNHNAHGYGVGSIERSLEIARTHLDFFAFTGHSS
                     WHDMPAMEGQRERHWIEGFKRLKDSWPRVQQVIAGGNRDGEFCAFLGFEWHSSQWGDQ
                     CVVFPEDHRPLHYAADPEKLRRFCIEERALMIPHHLAYPKGHRGVNWDVFREDCTPVV
                     EIFSEHGNAEDDRGPYPYVTHSLGGRETANTARAALDAGLRFGFVASSDDHAGFPGAY
                     GEGLMGALVGDFTRAGILEAIRARRTYALTGDRIEVEFGVDGAPMGAAIAAGRQAEVA
                     FEVRGRDELDVVEIVQDGRIVHRAHAGDAPPDFSRPVQLRLEWGWGPWGALALERICD
                     WAMQVKLTRGRIARFFPCLASMPFDEQRRHRFERQGEAGLAIRSYTSRRNAYRENPNQ
                     SVVLELETDAQASLALTLAEPVAQTVDATLADLARGSRHLFTGPFPKEGWQWHRIVPL
                     AASSVAGRCTLQVPAGRSSYVYLRARQKNGHMAWASPVFLN"
     gene            complement(3364..4125)
                     /locus_tag="VFB53_03910"
     CDS             complement(3364..4125)
                     /locus_tag="VFB53_03910"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85912.1"
                     /translation="MEPFIRLRGVSKVYRRGKSEHLAISDASFDVMPGELVSLVGPSG
                     CGKSTLLKILAGLHPHDGGEVKIGSAAHTFDPSRDVGMVFQAPLLLKWRRILENVLLP
                     AEILGLPAAEARERARGLLALVGLSGYEDKYPYELSGGMQQRAAIARALVHDPKLVLM
                     DEPFGALDALTREKMNLELLRIWKEAGKTIVFVTHGISEAVFLGSRVVVLTAGPARMA
                     DNFAIDLPHPRTLDMKTHERFGEYTRRIYKLLGME"
     gene            complement(4125..>4675)
                     /locus_tag="VFB53_03915"
     CDS             complement(4125..>4675)
                     /locus_tag="VFB53_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85913.1"
                     /translation="ALVAGVGLALAFTWSKPLESFCMPLLVSLNMIPKVALGPLIIVW
                     FKYGVFPNSIMAFSICVFPILLTTARGLREIEPELLDLVRSLKGSRWQLFSKVQLPGA
                     LPYIFSGMKVAAILAVAGAVVGEFLGSDKGLGYLMLQVQVTLDTPAMFMAVLLITLIG
                     MLLYGMVLALERWLVVQDARLD"
BASE COUNT          716 a         1636 c         1623 g          700 t
ORIGIN      
        1 gcgcccgcgg tccacgtata gccgtccttg gcggcgttca tcgcgttcat cgtgccgatc
       61 gagcccgcgc cgcccggctg gttgatcacc acgatcttgg cgccgagcgc gtcctcgagc
      121 tcgcccgcgg cgacgcgcgt cacctggtcg gtcgcccctc ccgcggccca cgggacgatg
      181 atgttgatcg gcttggtggg cttccattgc gcctgcgccg atagcgcggt cgcaagcgcg
      241 atcgcgccaa cgattgcttt cactgcgttc atggcttctc ccctttcctc atttggacag
      301 gtaacccagg acgagcgcgg tcatgtgctt aaggcgctcg gacttgttgc gcgacgacag
      361 caggtcgcgc gcgaaaatgg tcgacagtgt atggcggttg ctgagataga agtaaccgag
      421 cgccgcgatg gagatgtaca gctgcaccgg gtcgacgccc cgcctgaact tcccggtgcg
      481 ctcgccgcgg cgcagcaggt cgcgcagcag cgcgatcagc ggcgaatgca tcgccgcgat
      541 gtcgcgcgag cgcttgaggt gccgcgcgcg gtgcaggttc tcgctgttca gcagcgtcag
      601 gaactccggg tgcttcaggt agtagtccca ggtgaactcc accagccggc gcacgccctg
      661 caccggatcg aggtcgagca ggtgcaggcc cagctcggcc cggcggatgc gcgcatagct
      721 gtcctccagc accgcgagga acagcgcctc cttgtcgccg tagtagtagt acagcatgcg
      781 cttgttggcg ccggcgcgcg ccgcgatgcg gtcgacgcgc gccccgccca ggccgtagcg
      841 cgcgaactcg gccatcgccg ccttcaggat gcggtcctgg ttgcgcgcgg gatcgcggcg
      901 gccgttgcgc gccatggcgc ttattcggcc ttcacgccgg ccgaccgcgc gacggcagcg
      961 atcttggcca cctcggcggc gaggaagcgc ccgagctcct cgggggtgct gctcgccgca
     1021 tccgtgccga aggaggcgaa gcgctccttg atctcgggcg tcgccatgat gcgcacgatc
     1081 tcgccgttca ggcgctgcac gacctcctgc ggcaggccgg cggggccgac caccgcctgc
     1141 caggtcatga acgcgtattc gggatagccc gattcggcga aggtcggcac ctcgggcacc
     1201 gcgggcgagc gctgcggcgt ggtcaccgcg atggcgttca gccggccggc cttcacctgc
     1261 gtgagcacgt ccgagatgtt ggcgaacatc acctgcgccg tgccggcgag catgtcctgc
     1321 atcgccggcg cgccgccctt gtaatgcacc gtcacccagt cgatgccggc gatctgcttg
     1381 aagcgctcgc cggtgaggcg cgtgagcgtg gtggccccgg gagaagccat gttgagcctg
     1441 cccgggttcg ccttggcgta ggccaccagc tgctgcaccg tgcgcgccgg cactgcaggg
     1501 tgggtcacgg cgatgatcgg cacggtggcg acgagcgaca ccggcgtgaa atccttcacc
     1561 ggatcccagg acagcttggc gaagaacgcg ggcgcggtgg cgaacgaggt cggcgccatg
     1621 tacagcgtgt ggccgtcggg cgccgcgcgc gccacctgct cggcggcgat attgccgctc
     1681 gcgccggcct tgttctcgac cacgatcggc tggccgagcg cctgcgagag cttgtcgccc
     1741 agcgcgcgcg ccgcgaggtc ggtcgagccg cccggcggga agccgaccag catgcggatc
     1801 ggcttgctcg gatactgggc ccaggccgca gaggcgaaca acgcgaggaa cagcaatgcc
     1861 agtcttttca tggcgcgcac tccttcgatc gttagttgag gaacaccggg ctcgcccagg
     1921 ccatgtggcc gttcttctgg cgcgcgcgca ggtagacgta ggaggatcgg ccggccggca
     1981 cctgcagcgt gcagcggccc gcgaccgagg acgcggcgag cggcacgatg cggtgccact
     2041 gccagccttc cttcgggaac gggccggtga acaggtgccg cgagccgcgc gcgagatcgg
     2101 cgagcgtcgc gtccaccgtc tgcgcgacgg gttcggcgag cgtgagcgcc agcgaggcct
     2161 gcgcgtcggt ttccagctcc agcaccacgc tctggttggg attctcgcgg taggcgttgc
     2221 gccgcgaggt gtaggaacgg atcgccaggc ccgcctcgcc ctggcgctcg aagcggtggc
     2281 ggcgctgctc gtcgaacggc atgctggcaa ggcacgggaa gaagcgcgcg atgcggccgc
     2341 gcgtgagctt cacctgcatc gcccagtcgc agatgcgctc cagcgccagc gcgccccacg
     2401 gaccccagcc ccactccagg cgcagctgca ccggccggga gaaatccggc ggcgcgtccc
     2461 cggcgtgcgc gcgatgcacg atgcgcccgt cctgcacgat ctcgacgacg tcgagctcgt
     2521 cgcggccgcg cacctcgaac gcgacctcgg cctgcctgcc cgcggcgatg gccgcgccca
     2581 tcggcgcgcc gtcgacgccg aactccacct cgatgcgatc gccggtgagc gcgtaggtgc
     2641 gccgcgcgcg gatcgcctcg aggatgcccg cgcgcgtgaa gtccccgacc agcgcgccca
     2701 tcagcccttc gccgtaggcg ccggggaagc ccgcgtgatc gtcggacgag gcgacaaagc
     2761 cgaagcgcag ccccgcgtcc agcgccgcgc gcgccgtgtt cgcggtctcg cgcccgccca
     2821 gggaatgggt gacgtacgga tacgggccgc ggtcgtcctc ggcattgccg tgctcggaga
     2881 aaatctccac caccggcgtg cagtcctcgc ggaacacgtc ccagttcacg ccgcgatggc
     2941 ccttcgggta ggcgaggtgg tgcgggatca tgagcgcgcg ctcctcgatg cagaagcggc
     3001 gcagcttctc ggggtcggcg gcgtagtgga gcggccggtg gtcttcgggg aacaccacgc
     3061 actggtcgcc ccactgcgac gaatgccact cgaagccgag gaaggcgcag aactcgccgt
     3121 cgcggttccc cccggcgatc acctgctgca cgcgcggcca gctgtccttc agccgcttga
     3181 agccctcgat ccagtggcgc tcgcgctgcc cttccatggc cggcatgtcg tgccagctcg
     3241 agtgcccggt gaaggcgaag aagtcgaggt gcgtgcgcgc gatctccagc gaacgctcga
     3301 tcgaccccac cccgtagccg tgcgcgttgt ggttgtggat gtcgccgaac agcacgcggt
     3361 gcttcattcc atgccgagca gcttgtagat gcgccgcgtg tactcgccga agcgctcgtg
     3421 cgtcttcatg tcgagcgtgc gcgggtgcgg caggtcgatg gcgaagttgt ccgccatcct
     3481 cgccggcccg gcggtgagca ccaccacgcg cgagccgagg aacaccgcct ccgagatgcc
     3541 gtgggtgacg aacacgatgg tcttgcccgc ctccttccag atgcgcagca gctccaggtt
     3601 catcttttcg cgcgtcagcg cgtcgagcgc gccgaagggc tcgtccatca gcaccagctt
     3661 ggggtcgtgc accagggcgc gggcgatcgc cgcgcgctgc tgcatgccgc cggagagctc
     3721 gtaggggtac ttgtcctcgt agccggagag cccgaccagg gcgagcaggc cgcgcgcccg
     3781 ctcgcgcgcc tcggcggccg gcaggccgag gatctcggcg ggcagcagca cgttctccag
     3841 gatgcggcgc cacttcagca gcagcggcgc ctggaacacc atgccgacgt cgcgcgacgg
     3901 gtcgaaggta tgcgccgccg agccgatctt cacctcgccg ccgtcgtgcg ggtgcaggcc
     3961 ggcgaggatc ttgagcaggg tcgacttgcc gcagcccgag ggcccgacca gcgacaccag
     4021 ctcgcccggc atcacgtcga agctggcgtc cgagatcgcg aggtgctcgg acttgccgcg
     4081 gcggtagacc ttgctcacgc cgcgcaggcg gatgaacggt tccatcagtc gagcctcgcg
     4141 tcctgcacca ccagccagcg ctccagcgcc agcaccatgc cgtagagcag catgccgatc
     4201 agcgtgatga gcagcaccgc catgaacatc gccggcgtgt cgagggtcac ctgcacctgc
     4261 agcatgaggt agcccagccc cttgtccgag ccgaggaact cgcccaccac cgcccccgcc
     4321 acggcgagga tcgccgccac cttcatgccg gagaagatgt acggcagcgc gccgggcagc
     4381 tgcaccttgg aaaacagctg ccagcgcgag cccttcaggg accgcaccag gtcgagcagc
     4441 tcgggctcga tctcgcgcag cccgcgcgcc gtggtgagca ggatcgggaa cacgcagatg
     4501 gagaacgcca tgatggaatt ggggaacacg ccgtacttga accagacgat gatcagcggc
     4561 ccgagcgcca ccttggggat catgttgagc gacaccagca gcggcatgca gaacgattcc
     4621 agcggcttgg accaggtgaa ggcgagcgcc agccccacgc ccgccaccag cgcga
//
LOCUS       DASRIE010000085        17897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3479475, whole genome shotgun sequence.
ACCESSION   DASRIE010000085 DASRIE010000000
VERSION     DASRIE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479475"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFB53_03920"
     CDS             complement(<1..849)
                     /locus_tag="VFB53_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN85914.1"
                     /translation="MNKPVDTAAATMTGAEAVVELLKAHGVEVIFGLCGDTSLPLYDA
                     LARLPHGMRHVLTRDERHAAYMADGYARVTGKVGVCEGPSGGGATYLLPGLVEANESS
                     IPVLGINTDVSVSSRGRFTLTELDQQALMRPLTKWNAVLDRAADIPRTFRAAFNAMTT
                     GRPGAAHIALPFDVQNGPVERSDVWGDPSLGSYPSRRVAPESFHIELAAKLLRSAKRP
                     VFICGGGPVSAGAQDELLELAVKLGAPVATTISGQGSISEEHPLAVGVVGSNGGTPET
                     RSIVDQA"
     gene            869..1726
                     /locus_tag="VFB53_03925"
     CDS             869..1726
                     /locus_tag="VFB53_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN85915.1"
                     /translation="MQAADRQRPPPGELYLDHVSHFVPDLGKAAALLEQLGFAVTPDS
                     AQATQDGPAGTSNRCVMLERGYLEFLTPTMDTPNAARMRKSMRRYRGAHLVCFGTPAA
                     EDEHFRLRNHGFAPQPLVHLERKVEGGLTARFSVVRAAPGKMPEGRVQYVQHLAPEAI
                     WRPQHLKHRNGVLGLAAAYVVAADPVRAAARWARFMAGLPRREGKFVSLQTDRGKVLI
                     GTRAQMKALLGAAPPSPGIAGYAIACKDPQAFARRCKAAGLAVNRNLAVVLPPALGGA
                     WRLVPREKP"
     gene            1723..3132
                     /locus_tag="VFB53_03930"
     CDS             1723..3132
                     /locus_tag="VFB53_03930"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN85916.1"
                     /translation="MKQDVCALSATELLQLYRTKSLSPVEAARAVLARIAELNPVLNC
                     FNLIDAKSALDAARASEARWMKGAPMGPLDGVPASIKDIILTRGWPTRRGSKTTDPAG
                     PWNDDAPAVARLREAGAVLVGKTTTPEFGWKGVTDSPLTGITRNPWNPKKTPGGSSGG
                     SAAAVAAGMGPLTVGTDGGGSIRIPCGFTGLFGLKPSFGRVPAWPLSPFGTVAHLGPM
                     TRTVSDAALMLNVLAQPDARDWTSLPYDKRDYLAGLEDGVQGLRIAYSPTLGYARVDK
                     EIAALVKSAVEVFANLGAKVEEVDPGFDNQLEVFTLHWFPGAAYVVRGIPAAKRKLMD
                     PGLLDVARQGEKITLKQVQDAVAKRGALGVLMNKFHQKYDLLLTPTLPVPAFDAGREF
                     PGAGGKRRWTDWTPFSYPFNLTQQPAASIPCGLTRAGLPAGLHIVGPKYGDAQVLRAA
                     RAFEEVRPLVMPDLAKLAR"
     gene            complement(3129..3836)
                     /locus_tag="VFB53_03935"
     CDS             complement(3129..3836)
                     /locus_tag="VFB53_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN85917.1"
                     /translation="MSRPATPGLLARALPLAFAAALGAAAQAQVPPTGAEVAAYAGAH
                     AAAARGDAAGIAVLAKGGADLHARDRYRRTPLHVAAYRAQREAMRALAKAGADANALE
                     HDRYDIVTIAAVADDVATLQVALEIGCSAKNVTSRYEGTALIAAAHLGHDEVVRVLIR
                     AGAPLDHVNNLGWTALIESIVLGNGGARHTATLKALVGAGADVNLADRNGRRPLALAR
                     ERGYREMAAILAAAGAR"
     gene            complement(3833..4270)
                     /locus_tag="VFB53_03940"
     CDS             complement(3833..4270)
                     /locus_tag="VFB53_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85918.1"
                     /translation="MTGHTFLFREGTWRAAGEYRDGGGNVTKVDGETRVRHDAGKWLT
                     ESVLRIKSSPPREQGNRTEVVPFSPGSNATHWSSESATLGSLNGRFVVAGDAILSFYA
                     SGTGRYRGFECMQQKDAKHYRVRGAMLEQDKVISTWVVELTAA"
     gene            4294..5892
                     /locus_tag="VFB53_03945"
     CDS             4294..5892
                     /locus_tag="VFB53_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN85919.1"
                     /translation="MEPRHFQHWPPDMPRGLALPPLRLHDLLARAAARLPDKPATVFE
                     GVTLSYREILRRSEALAGHLQRACGVRKGDRVLLDLQNGHDYVVALFGILRADAVAVP
                     LSPANVAAELKHYLQDSGAKVSVVEGDVAARFAGLPLEHLVVMDGFRGGAEASPSTAR
                     SDDLCLLPYTSGSTGQPKGCMHTHASCLHNIAGAALWKHIGEDTVALATAPFFHVTGL
                     IHSFLATVYAGGTMVIQRRWDPLAAARLVARHRCTHWDNVPTMVVDLLSHPQALEHDV
                     SSLKWIFGGGAAMPEAIAQKLFELCGVRYVEGYGLTETISQTHINPPQNPKQQCLGIP
                     TFDTEALVVDPESLRILGPGEQGEILVRGPQLLRGYWGNERATRESRVEVGGKEYFRT
                     GDLGRTDADGYYFISDRLKRMINAAGFKVWPAEVEAAMYQHPAIKECCVIAAPDERRG
                     ETVKAVVVLRPGAAASAQEIVDWARGRMAAYKAPRLVAFTDALPRTGSGKLMWRALQE
                     QEWGIPGGKTAPGGSARGQARQKL"
     gene            complement(5880..6932)
                     /locus_tag="VFB53_03950"
     CDS             complement(5880..6932)
                     /locus_tag="VFB53_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZN85920.1"
                     /translation="MRVPRKKKLLLGAVSAACALPALALAQAPAGVEIYGRANVSYER
                     ITVTNAPTDWEVVDNSSRIGFRGKKDLGDGLSAFFQIESRVRLDNGGDTFSSRDSYLG
                     MSGGFGTARVGRTIGPVYYATYDYISNHNHDTGNSSDALLALAIFGNQGFMNNTVWYT
                     SPKMGAFTVDVAYSRLGEARAAPGMSQPSYMGLVGAYDQGPLHVAVSHAETKNSADLG
                     GGIGNKDTATTIGGLYNFGGKVIVGALYEKAKSDQLVGDTQRDYFRVSVMVPVAKHEF
                     HVNLGMVDDRLDVNMADAGAKQYTLAYNYNVTKEFKLYAFYTVVDNEDNGNYGFKTNV
                     AGIDNKSFAVGARYNF"
     gene            complement(7033..8256)
                     /locus_tag="VFB53_03955"
     CDS             complement(7033..8256)
                     /locus_tag="VFB53_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85921.1"
                     /translation="MGRGDGRAAGGPARAEILTRAVFLLSLAAFASAAASRATDPVLP
                     LIAASFGATPGEAAAVITGFALAYGLLQLVNGPLADRIGKFRMVFWITAVSAAGNLAC
                     ALAPSLPLLVGARFATGATVGAIVPLAMAWIGDTVPYERRQAVLARFLIGHMLGVALA
                     TAGAGFLGERYGWQAMFYVLAVLYALIAALLRLELGRNALTREAHADSGSLGDAFRRM
                     AALTRRPWVRVVLATVFVEGALFSGASAFIPLELHLRFGLGLGASGSIVAASVAGSLL
                     YASMAVRVVPRLGERGLVLGGGAFLACGFLGLAAAPAAGWALPCMVALGAGFYMMHNT
                     LQVHATQMAPEARGAALALFACSLFTGQAAGVWLASHVVDAAGAGPVFVAVAIGLALL
                     SLDFRRRLAAPRPGA"
     gene            complement(8202..8816)
                     /locus_tag="VFB53_03960"
     CDS             complement(8202..8816)
                     /locus_tag="VFB53_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN85922.1"
                     /translation="MIDLYAAGTSNGMRARIALEECGLPYEFHPVALDKGEHKTPQFL
                     AMNPNGQIPVIVDRDAAGGKPVTLSQSTAILVYCAEKSGKYMPRDAAARAAMLEALMN
                     ASTDITPIFGAYFGIVRSKEPHAPSAQLFKDRLRAFFKVWDDKLAKRKCAAGDEVTIA
                     DFSLYAGYARIKGAAPDILEGLPNVTRWAEAMAARPAVQRALKF"
     gene            complement(8833..10317)
                     /locus_tag="VFB53_03965"
     CDS             complement(8833..10317)
                     /locus_tag="VFB53_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN85923.1"
                     /translation="MSAWIPRTLGEVLAAQDPAAAALVTATARLTYGGLLARARQAAG
                     SLQALGLGKGDHVALLMGNDEHWLALFYGAALLGAVTVPVNTRFKAAELEFCLRQADC
                     RALLYAPRFLNNDYEAMLPSIRPRLPKLAHAVRVGELRAAPFAPVEVAPEDLLLIQFT
                     SGTTAYPKGVMLMHDGMLRNAWAAGARMGVQAADRYFNCRPFFHVGGSTLSALVCLVH
                     GACLVTLPSFEAGAALEMMEQERCTLTSGNDTIFQLLMGHEDFPRRKLALRGGWAAAG
                     PQTMRRIIDVLGAKHICAAYGLSEASPNVVMSDWRDAEELRVQGLATPHQGLHLRTVD
                     GEIQVRGWSVMRGYYKDPTATAKAFTEDGYLRTGDLGELHPGGRLRMVGRIKEVFRVG
                     GENVAPAEVEEVLLGHPAVETAQVVGVPDARLGEVPCAYVSLKAGQAATEDALLAWCK
                     SRSANFRVPRYLRIVDNFESIGMTASGKVQKARLRERALKDFNL"
     gene            complement(10314..11027)
                     /locus_tag="VFB53_03970"
     CDS             complement(10314..11027)
                     /locus_tag="VFB53_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85924.1"
                     /translation="MTLLCETKDGVATLTLDRPGSRNALDLALCEALRAAAQGMDASV
                     RLVLVRANGPVFCAGADLKERQGMDAAQVRERRMKAFAAYAALEALAMPAVAVIEGPV
                     VGSGCEIAAACDFIVATPAASFRIPEALRGTVGATQRLPRILGKRLAKDLMFTGRTLT
                     AEEAERHGLVSRLVAPEKLDAVIAGIAKTIVGAPAQALRLAKRSIDRGVELDPQGALA
                     TEIAAIDEQLAAGDWMGRR"
     gene            complement(11024..12208)
                     /locus_tag="VFB53_03975"
     CDS             complement(11024..12208)
                     /locus_tag="VFB53_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN85925.1"
                     /translation="MKPLEHLTILDLSRVLACPFASMILAELGAAVIKVEQPGAGDET
                     RGFEPQLGRDSAYYFACNRSKASITVNLRSDEGRRIIRGLAQQADVLVENFPVGTLGR
                     QGLDYATLSALNPKLVYVSCTGFGQTGPYAQKKGYDTVFQAMGGLMSLTGEKGGGPVK
                     PGLPVADLSSGLWVAIAILAALSGRERTGRGAQVDFSMLDGQVSLLTLAAARYFALGE
                     VPPRLGTEHPGRVPSATFRCKDGKYAHITASDQHWQPLCDALGLEKMGSDPIFSANAA
                     RVARRGEVMTELTRAVEKLTRAELIAKLDAADVPVGPVNDVAEVLADPQVRARRMVGS
                     FDYPGVGEFKALALPYKFLGWDDPQIGRPPALGEHTAAILKERLGYSDERIAALREAK
                     AI"
     gene            complement(12210..13097)
                     /locus_tag="VFB53_03980"
     CDS             complement(12210..13097)
                     /locus_tag="VFB53_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN85926.1"
                     /translation="MGITYALLAMLCFATNIMITRYAVARLPVEAGFFIVLATNVLFP
                     AALFAAELAVRAAPYAWNWKGAGLFAIGGVIGTFLGRRFLFDAVRILGPSRASVFHST
                     APAFALVAAWLLADEVLGAYEIALMALVWFGLWLTQPRAGSVQALSPEMLRKGMLVGL
                     LTVAGFSFGNVLRGLAMRVWDEAALGTVVASLAALACQFVVTRDWAGIAAQLRAADRK
                     AVLLYTGCGVFTSLGSIFISLAMTRLEIALAVLVVHTTPVVIFPVSVWLLKNREALNA
                     RTVLGAALVLAGIAFLAWR"
     gene            complement(13101..13820)
                     /locus_tag="VFB53_03985"
     CDS             complement(13101..13820)
                     /locus_tag="VFB53_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85927.1"
                     /translation="MIIVRDQGAVRVLTLNRPEKRNALNTALTQALLDALRAADADEA
                     VGCVVLTGAGQGFCAGADLAEFKELTPGNQPLVEARAELSMQLHLAFPGMAKPVVTAI
                     NGAAMGGGAGLAIAGDLAVMAEGATLGYPETRHGIVAAIVMANLVRQAGRKAAFELVG
                     LGEPVGAQRALALGLVNRVVPPERLLDEALALAQKLAAASRPAMRLTKELFHEAADLP
                     LAAALERGREANKRMRSFRKP"
     gene            complement(13817..14761)
                     /locus_tag="VFB53_03990"
     CDS             complement(13817..14761)
                     /locus_tag="VFB53_03990"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZN85928.1"
                     /translation="MKTRITELLGIRHPVFQAAMSWASSSAPLVIAVSNAGALGVIAA
                     GPMRNDEFRRSLAAIKSGTDQPYGVNIPLNNPRSADLLDIAFEARIPVMVASQGGPKE
                     QLARFRAIGARWLHVVASVEHARKAEAAGVDALVVDGMEAGGHPPPSEVSTLVLVRRV
                     VKATKLPVVASGGVADGAGIAALLALGADAVQLGTRFIATPEASVHDNYKRAVLAADV
                     DGSTLVARGKLPVRQLKNAFTAKFDAAERAGAPQAELDALFRAATLKQAALDGDVEWG
                     KVEAGQSAGLVNEILPAAEVVKRLMVELEEARKRLAAL"
     gene            complement(14758..15243)
                     /locus_tag="VFB53_03995"
     CDS             complement(14758..15243)
                     /locus_tag="VFB53_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN85929.1"
                     /translation="MKPRSSTIDAPGIRDLLSYRLHVVANLLSRGAELRYRREFGVSL
                     WEWRSVALLGGAAGAQSLNDLARAAGIDKSQMSRVVSGLAARRLVLRAIDAEDGRGVR
                     LTLTRAGRALYQGLMRAAAERNDAFLDCLSAKERAAFDAALGKLAARARAFIEREGAP
                     R"
     gene            complement(15218..15769)
                     /gene="pcaG"
                     /locus_tag="VFB53_04000"
     CDS             complement(15218..15769)
                     /gene="pcaG"
                     /locus_tag="VFB53_04000"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HZN85930.1"
                     /translation="MIPTGEATLGPFFPPRYVDAGANDLTQCGGRKAGGEAIEIVGCV
                     RQEDGAPLDNLLLEIWQADANGIYRHRADPRHREADADFLGWGRAATDKAGNYAFRSV
                     RPGAPAGRARHVNFMVMFSGLMRILKTTMFFPGEQANGKDPVYQAVPEARRALLVARE
                     EAPGRYRFDLKLRGEHETPFFDD"
     gene            complement(15766..16464)
                     /locus_tag="VFB53_04005"
     CDS             complement(15766..16464)
                     /locus_tag="VFB53_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HZN85931.1"
                     /translation="MDYRIAPRDERTQPARIVPGYPQTPWRNPREPLVRRAPTLSELT
                     GPAGVEKRVQLLGGDIAGRGETQAIGQLMHLRVRVVDEDGAPVGGAVVEIWQCNAAGR
                     YIHPNDDEHAPVDRNFYGAARLVTDESGAFTLRTVKPGAYPVPTKDGWWRPPHIHFSL
                     FGKVWLSRLVTQMYFPGEPLNPQDRILNAVPDAAARERLIARLVPPAGEPHGALVFEH
                     QIVLRGSQATPVQP"
     gene            16495..17415
                     /locus_tag="VFB53_04010"
     CDS             16495..17415
                     /locus_tag="VFB53_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN85932.1"
                     /translation="MSTMADVTLCECFARDGLQHEPAFIATDVKRALVERFAALGFRR
                     IEATSYSNPKVVPQFADASELLRSLPRRDGAHYKATCANPKAVERALADLDAGFGVNE
                     ISLLVSATEAHTQKNLKRTRAGQWANVEQMVQLAGGRFRLVGTVSVAFGCPFEGKVDP
                     GVVAADVAKFVSLGVAHVALGDTTGLATPTSVKDMVRRMPAGAIPIAHFHDTRGTGLA
                     NYVAAHEAGVRFFDASFGGVGGHPAKVKYGGGHTGNVCTEDLVNLFETMGIDTGIDLD
                     KLLAAAEYCEQVLGRELHGRVTRSGLGPRR"
     gene            17412..>17897
                     /locus_tag="VFB53_04015"
     CDS             17412..>17897
                     /locus_tag="VFB53_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZN85933.1"
                     /translation="MKPPYRADQVGSLLRPAELSAARKEWKQGALAADALKVIEDRAI
                     REAVAKQEALGLQAVTDGEFRRDWWHLDFLAQLDGVTTRENPGPKFGGTEEQPPIATV
                     TGKVGCSRPIMVEHFAFLKSVAKATPKFTIPSPSMLHLRGGRNAISRDAYPDLEDFWE
                     DV"
BASE COUNT         2596 a         6466 c         6309 g         2526 t
ORIGIN      
        1 ggcctggtcc acgatcgagc gcgtctccgg cgtgccgccg ttggatccca ccacccccac
       61 ggcaagcgga tgctcctctg agatcgagcc ctgcccgctg atggtggtcg ccaccggggc
      121 gccaagtttt actgccagct ccagcagctc gtcctgcgcg ccggcgctca ccggcccgcc
      181 gccgcagatg aacaccgggc gcttcgcgga acgcagcagc ttcgccgcga gctcgatgtg
      241 gaacgattcc ggcgctacgc gccgcgaggg atacgagccc agcgaaggat cgccccagac
      301 gtccgaccgc tccacgggcc cgttctgcac gtcgaagggc agcgcgatgt gcgccgcccc
      361 gggcctcccc gtggtcatgg cattgaacgc ggcgcggaag gtgcgcggaa tgtccgcggc
      421 gcggtcgagc accgcgttcc acttggtgag cgggcgcatg agcgcctgct ggtcgagctc
      481 ggtgagggtg aagcgcccgc gcgaagacac ggacacgtcg gtgttgatgc cgagcaccgg
      541 gatggaggac tcgttcgcct ccaccagccc cggcaggagg taggttgccc cgccgcccga
      601 aggcccttcg cacacgccga ccttgcccgt cacccgcgca tagccgtcgg ccatgtaggc
      661 ggcgtgccgc tcgtcgcgcg tcagcacgtg ccgcatgccg tgcggcaggc gcgcgagcgc
      721 gtcgtacagg gggaggctgg tatccccgca caggccgaag atcacctcca cgccgtgcgc
      781 cttgagcagt tccacgaccg cttccgcgcc cgtcatcgtt gcagccgcgg tatcgaccgg
      841 cttgttcatg cagttatatt agcgcgacat gcaagccgca gaccgccagc gcccgccccc
      901 gggcgagctc tacctggacc atgtttcgca tttcgtgccc gacctcggca aggcggcggc
      961 gctgctggaa caactcggct tcgccgtcac gccggattcg gcgcaggcga cgcaggacgg
     1021 ccccgcgggc acctcgaacc gctgcgtgat gctggagcgc ggttacctcg aattcctcac
     1081 gccgacgatg gacacgccga acgccgctcg catgcgcaaa tccatgcggc gctaccgcgg
     1141 cgcgcacctc gtgtgcttcg gcacgccggc cgcggaggac gagcatttcc gcctgcggaa
     1201 ccacggcttc gcgccgcagc cgctggtgca tctggagcgc aaggtggaag gcggcctgac
     1261 ggcgcgtttt tcagtggtgc gcgccgcccc cggcaagatg cccgaaggcc gcgtgcagta
     1321 cgtgcagcac ctcgcgccgg aggcgatctg gcggccgcag cacctgaagc accgcaacgg
     1381 cgtgctcggc ctcgccgcgg cctacgtcgt cgccgccgac ccggtgcgcg ccgcggcgcg
     1441 ctgggcgcgc ttcatggccg gcctgccgcg ccgcgaggga aagtttgtgt cgctgcagac
     1501 agaccgcggc aaggtgctga tcggcacgcg cgcccagatg aaggcgctgc tcggcgccgc
     1561 gccgccctcg cccggcatcg ccggctacgc catcgcctgc aaggacccgc aagcctttgc
     1621 gcgccgctgc aaggcggccg ggctggcggt caaccgcaat ctcgccgtcg tgctgccgcc
     1681 cgcgctgggc ggcgcctggc ggctcgttcc cagggagaag ccatgaaaca ggatgtctgc
     1741 gcgctgagcg ccaccgaact tctgcagctc taccgcacca agtcgctctc gcccgtcgag
     1801 gccgcgcgcg cggtgctcgc gcgcatcgcc gaattgaacc cggtgctcaa ctgcttcaac
     1861 ctcatcgacg cgaaatccgc gctggatgcg gcgcgggcga gcgaagcgcg ctggatgaag
     1921 ggcgcgccca tggggccgct ggacggcgtg ccggcctcca tcaaggacat catcctcacc
     1981 aggggctggc cgacgcggcg cggctcgaag accaccgatc ccgccggccc ctggaacgac
     2041 gatgcgcccg cggtggcgcg cctgcgcgaa gcgggcgcgg tgctggtggg caagaccacc
     2101 acgccggaat tcggctggaa aggcgtcacc gacagcccgc tcaccggcat cacgcgcaat
     2161 ccctggaacc cgaagaagac gcccggcggc tcttctggcg gcagcgcggc ggcggtggcc
     2221 gccggcatgg gcccgctcac cgtcggcacc gacggcggcg gctcgatccg catcccctgc
     2281 ggcttcaccg gattgttcgg cctgaagccc tccttcggcc gcgtgcccgc ctggccgctg
     2341 tcgcctttcg gcaccgtggc gcacctcggg ccgatgacgc gcacggtgag cgacgcagcg
     2401 ctgatgctga acgtgctcgc gcagcccgat gcgcgcgact ggacctctct gccctacgac
     2461 aagcgcgact acctggccgg cctcgaggac ggcgtgcagg gcctgcgcat cgcctattcg
     2521 ccgaccctgg gctacgcccg ggtggacaag gagatcgcgg cgctggtgaa aagcgccgtc
     2581 gaggtgttcg cgaacctcgg cgcgaaagtg gaggaagtgg acccggggtt cgacaaccag
     2641 ctggaggtgt tcacgctgca ctggttcccc ggcgccgcgt acgtggtgcg cggcatcccc
     2701 gcggcgaaga ggaagctcat ggacccgggg ctgctcgatg tggcgcgcca gggggagaaa
     2761 atcacgctga agcaggtgca ggacgcggtg gcgaagcgcg gcgcgctcgg cgtgctgatg
     2821 aacaaattcc accagaagta cgacctgctc ctcacgccga cgctgccggt gcccgcgttc
     2881 gacgccggca gggagttccc gggcgcgggc ggcaagcgcc gctggaccga ctggacgccc
     2941 ttttcctacc ccttcaacct cacgcagcag ccggcggcgt ccattccctg cgggctgacc
     3001 agggcggggc tgccggcggg gctgcacatc gtcgggccga agtacggcga cgcgcaggtg
     3061 ctgcgcgcgg cgcgcgcctt cgaggaggtg cggccgctcg tgatgccgga cctggcgaag
     3121 ctcgcgcgct agcgcgcgcc cgctgctgcc aggatcgcgg ccatttcccg gtagccgcgc
     3181 tcgcgcgcca gcgcaagcgg cctgcggcca ttgcggtccg cgagattcac gtccgcgccc
     3241 gcgccgacca gcgccttcag cgtcgccgtg tgccgcgcgc cgccattgcc gagcacgatg
     3301 gattcgatca gggccgtcca gccgaggttg ttcacgtggt cgagcggcgc gccggcgcgg
     3361 atgagcaccc gcaccacttc gtcgtgcccc aggtgcgccg cggcgatgag cgcggtgccc
     3421 tcgtagcggc tggtgacgtt cttcgcgctg cagccgatct cgagcgcgac ctggagggtc
     3481 gccacgtcgt cggccaccgc ggcgatggtg acgatgtcgt agcggtcgtg ctccagggca
     3541 tttgcatcgg cgcccgcctt cgccagcgcg cgcatcgcct cgcgctgcgc gcgatacgcc
     3601 gcgacgtgca gcggcgtgcg ccggtaccgg tcccgcgcgt gcaggtcggc gcctcccttg
     3661 gccagcactg cgattccggc ggcgtcgccg cgcgcggcgg cggcgtgcgc tccggcgtag
     3721 gcggcgacct cggcgccagt gggcgggacc tgcgcctgcg cggcagcgcc aagcgcggcg
     3781 gcgaaggcga gcggcagcgc gcgcgccagg aggcccggcg tcgccggccg gctcatgccg
     3841 ccgtgagctc gaccacccag gtcgaaatca ccttgtcctg ctcgagcatc gcgccacgca
     3901 cccggtagtg cttcgcatcc ttctgctgca tgcactcgaa gccgcggtag cggccggtgc
     3961 cgctggcgta gaaggacagg atggcgtctc ccgcgaccac gaagcgcccg ttgagcgagc
     4021 cgagcgtggc gctctcggag gaccagtgcg tcgcattcga gccgggcgag aacggcacca
     4081 cctcggtgcg gttgccctgc tcgcgcggcg ggctgctctt gatgcgcagg acgctttcgg
     4141 tgagccactt gcccgcgtcg tggcgcacgc gcgtctcgcc gtccaccttg gtgacgttgc
     4201 cgccgccgtc gcggtactcg cccgcggcgc gccacgtgcc ctcgcggaag aggaaagtgt
     4261 gacctgtcat gggggcaact atacttcccg cccgtggagc cacgccactt ccagcactgg
     4321 ccgccggaca tgccgcgcgg cctggccctg ccgccgctgc ggctgcacga cctgctggcg
     4381 cgggccgccg cgcgccttcc cgacaagccg gcgacggtgt tcgaaggcgt aaccctcagc
     4441 taccgggaga tattgcgccg cagtgaggcg ctcgccgggc acctgcagcg ggcttgcggg
     4501 gtgcgcaaag gcgaccgggt gctgctcgac ctgcagaacg ggcacgacta cgtggtggcg
     4561 ctgttcggca tcctgcgcgc cgacgcggtg gcggtgccgc tgagcccggc caacgtcgcg
     4621 gcggaactga agcactacct ccaggacagc ggcgcgaagg tgtcggtagt ggaaggcgat
     4681 gtggcggcaa gattcgcggg attgccgctc gaacacctgg tcgtgatgga cggattccgc
     4741 ggcggcgccg aggcttcgcc ctccaccgcc aggtcggacg acctgtgcct gctgccctac
     4801 acctcgggca gcaccgggca gcccaagggc tgcatgcaca cgcatgccag ctgcctgcac
     4861 aacatcgccg gcgcggcgct gtggaagcac atcggcgagg acaccgtggc gctcgccacc
     4921 gcgccgttct tccacgtcac cggcctgatc cacagcttcc tcgccacggt gtacgccggc
     4981 ggcaccatgg tgatccagcg ccgctgggac ccgctcgccg cggcgcggct ggtcgcgcgg
     5041 caccgctgca cgcactggga caacgtgccc accatggtgg tggacctgct ctcgcacccg
     5101 caggcgctcg agcacgacgt ctcctcgctc aagtggatct tcggcggcgg cgcggcgatg
     5161 cccgaggcga tcgcgcagaa gctcttcgag ctgtgcggcg tgcgctacgt cgagggctac
     5221 ggcctcaccg agaccatctc gcagacgcac atcaacccgc cgcagaaccc gaagcagcag
     5281 tgcctcggca ttcccacctt cgacaccgag gcgctggtgg tggaccccga gagcctgcgg
     5341 atcctcggcc cgggagagca gggcgagatc ctggtgcgcg ggccgcagct gctcaggggc
     5401 tattggggga acgaacgggc cacgcgcgaa tcgcgcgtcg aggtcggcgg caaggagtac
     5461 ttccgcaccg gcgacctcgg ccgcaccgac gcggacggct actacttcat ctccgaccgg
     5521 ctgaaacgca tgatcaacgc cgcgggcttc aaggtctggc cggcggaagt cgaagccgcc
     5581 atgtaccagc accccgcgat caaggagtgc tgcgtcatcg ccgcgcccga cgagcgccgc
     5641 ggcgaaacgg tgaaggcggt ggtggtgctc aggcccggcg ccgcggcgag cgcgcaggag
     5701 atcgtggact gggcgcgcgg ccgcatggcg gcctacaagg ccccgcgcct ggtcgcgttc
     5761 acggacgcgc tgccgcgcac cggctcgggc aagctcatgt ggcgcgcgct gcaggagcag
     5821 gagtggggaa tacccggcgg gaaaacggcc ccgggcggat ccgcccgggg ccaggcgcgt
     5881 cagaagttgt agcgcgcgcc gacggcgaag gacttgttgt cgatgccggc aacgttggtc
     5941 ttgaagccgt agttgccgtt gtcctcgttg tcgacgacgg tgtagaacgc gtacagcttg
     6001 aactccttgg tgacgttgta gttgtaggcc agcgtgtact gcttggcgcc ggcatcggcc
     6061 atgttgacgt cgagccggtc gtcgaccatg cccaggttga cgtggaactc gtgcttggcc
     6121 accggcacca tcaccgagac gcggaagtag tcgcgctgcg tgtcgccgac cagctggtcg
     6181 ctcttcgcct tctcgtacag cgcgccgacg atcaccttgc cgccgaagtt gtacaggccg
     6241 ccgatggtgg tggcggtgtc cttgttgccg atgccgccgc cgaggtcggc agagttcttg
     6301 gtctccgcgt gcgacaccgc gacgtgcagc gggccctggt cataggcgcc gaccagcccc
     6361 atgtagctcg gctgcgacat gcccggcgcg gcgcgcgcct cgcccaggcg cgaatacgcc
     6421 acgtccacgg tgaaggcgcc cattttcggg ctggtgtacc agacggtgtt gttcatgaag
     6481 ccctggttgc cgaagatcgc cagcgccagc agcgcgtccg aggaattccc cgtgtcgtgg
     6541 ttgtggttgc tgatgtagtc gtaggtggcg tagtacaccg gtccgatggt gcgcccgacc
     6601 cttgcggtgc cgaagccgcc cgacatgccg aggtagctgt cccggctcga gaacgtgtcg
     6661 ccgccgttgt ccagcctgac ccggctctca atctggaaga acgcggacag gccgtcgccc
     6721 aggtccttct tgccgcggaa gccgatgcgg gaactgttgt ccaccacttc ccagtccgtc
     6781 ggcgcgttgg tgacggtgat gcgctcgtag ctgacgttgg cgcggccgta gatctcgacc
     6841 ccggccggcg cctgcgccag cgccagcgcg ggcagcgcgc aggcggcgct caccgcgccg
     6901 agcagtagct tcttctttct tgggactctc atgacggctc tcctttggtt ggatgacccc
     6961 cttgcgattc ccccccgccg cgctactgta ctgagaatcc ggatcgccgc gcaaccatcg
     7021 gcggaagcga ggtcatgcgc ccgggcgcgg cgcagcgagc cgccgccgga agtcgagcga
     7081 cagcagcgcc aggccgatgg ccaccgccac gaacaccggt ccggcgcctg ccgcgtcgac
     7141 cacatgcgag gcgagccaca cgcccgccgc ctgcccggtg aacaggctgc aggcgaacag
     7201 cgccagcgcc gcgccgcgcg cctccggcgc catctgcgtg gcgtgcacct gcagcgtgtt
     7261 gtgcatcatg tagaacccgg cgcccagcgc caccatgcag ggcagcgccc agccggccgc
     7321 gggggcggcg gcgaggccga ggaacccgca ggcgaggaat gcgccgccgc ccagcaccag
     7381 gccgcgctcc cccagccgcg gcacgacgcg caccgccatc gaggcgtaca gcaggctgcc
     7441 ggcgacggac gccgccacga tggagccgct cgcgcccagg ccgaggccga agcgcaggtg
     7501 cagctcgagg ggaatgaagg cgctggcgcc gctgaacagc gccccctcga cgaacacggt
     7561 cgccagcacg acgcgcaccc aagggcggcg cgtcagcgcc gccatgcggc ggaaggcgtc
     7621 gccgagcgag ccgctgtcgg catgcgcctc gcgcgtcagc gcattgcggc cgagctccag
     7681 ccgcagcagc gcggcaatga gcgcgtagag gactgccagc acgtagaaca tcgcctgcca
     7741 gccgtagcgc tcgccgagga agcccgcgcc cgcggtggcg agggccacgc cgagcatgtg
     7801 gccgatgagg aagcgcgcga gcaccgcctg gcggcgctcg tagggcaccg tgtcgccgat
     7861 ccaggccatc gccagcggga cgatggcgcc gaccgtggcg ccggtggcga agcgcgcgcc
     7921 gaccagcagg ggcagcgagg gcgcgagcgc gcaggcgagg ttgccggcgg ccgagaccgc
     7981 cgtgatccag aacaccatgc ggaacttgcc gatgcggtcg gccagggggc cgttgaccag
     8041 ctgcagcagc ccgtaggcca gggcgaagcc ggtgatcacc gcggcggcct cgcccggcgt
     8101 ggcgccgaag ctcgccgcga tcagcggcag caccgggtcg gtggcgcgcg aggccgccgc
     8161 gctcgcgaag gcggcgagcg aaaggaggaa gacggcgcgc gtcagaattt cagcgcgcgc
     8221 tggaccgccg gccgcgcggc catcgcctcg gcccagcgcg tcacgttcgg caggccttcg
     8281 aggatgtcag gcgccgctcc cttgatgcgg gcgtagcccg cgtacaggga gaagtccgcg
     8341 atcgtcacct cgtcgccggc ggcgcacttg cgcttcgcca gcttgtcgtc ccacaccttg
     8401 aagaaggcgc gcaggcggtc cttgaacagc tgcgccgacg gcgcgtgcgg ctccttggag
     8461 cgcacgatgc cgaaataggc gccgaagatc ggcgtgatgt cggtggaggc gttcatcagc
     8521 gcttccagca tggcggcgcg cgcggcggcg tccctcggca tgtacttgcc cgatttctcc
     8581 gcgcagtaga cgaggatcgc ggtggactgc gacagcgtca ccggcttgcc gcccgcggcg
     8641 tcgcggtcga cgatcacggg aatctggccg ttcgggttca tggcgaggaa ctggggcgtc
     8701 ttgtgctcgc ccttgtccag cgccaccggg tggaactcgt agggcaggcc gcattcctcg
     8761 agggcgatgc gggcgcgcat gccgttggag gtgccggcgg cgtacaggtc gatcatggtt
     8821 gttcccttct ctctacaggt tgaaatcctt cagcgcacgc tcgcgcagcc tggctttctg
     8881 caccttgccg ctggcggtca tgccgatgga ttcgaaattg tccacgatcc tgaggtagcg
     8941 cggcacgcgg aagttcgcac tgcgggactt gcaccacgcc agcagcgcgt cctcggtcgc
     9001 cgcctgcccc gccttgagcg acacgtaggc gcagggcact tcgcccagcc tcgcatccgg
     9061 caccccgacc acctgcgccg tctccaccgc cgggtgcccg agcagcacct cctccacctc
     9121 cgcgggcgcc acgttctcgc cgccgacgcg gaacacctcc ttgatccgcc ccaccatccg
     9181 caggcgcccg cccggatgca gctcgcccag gtcgccggtg cgcaggtagc cgtcctcggt
     9241 gaaggccttc gcggtcgccg tcgggtcctt gtagtagccg cgcatcacgc tccagccgcg
     9301 cacctggatc tcgccgtcca ccgtgcgcag gtgcaggccc tggtgcggcg tggcgagacc
     9361 ctgcacgcgc agctcctcgg catcgcgcca atcgctcatc accacgttgg gcgaggcttc
     9421 cgacaggccg taggcggcgc agatatgctt cgcgccgagc acgtcgatga tccggcgcat
     9481 ggtctgcggc ccggccgccg cccagccgcc gcgcaaggcg agcttgcgcc tgggaaaatc
     9541 ctcgtgcccc atcagcagct ggaagatggt gtcgttgccc gaggtgagcg tgcagcgctc
     9601 ctgctccatc atctccagcg ccgcgcccgc ttcgaagctg ggcagcgtga cgaggcaggc
     9661 gccatgcacc aggcacacca gggccgacag ggtggagccg ccgacgtgga aaaagggccg
     9721 gcagttgaag tagcggtcgg cagcctgcac gcccatgcgc gcgcccgcgg cccaggcatt
     9781 gcgcagcatg ccgtcgtgca tgagcatcac gcccttgggg taggccgtgg tgccggaggt
     9841 gaactggatg aggagcaggt cctccggcgc cacctcgaca ggcgcgaagg gcgcggcgcg
     9901 cagctcgccc acgcgcacgg cgtgcgcgag cttcggcagc ctgggccgga tcgacggcag
     9961 catggcctcg tagtcgttgt tgaggaagcg cggcgcgtag aggagcgccc tgcaatccgc
    10021 ctgccgcagg cagaactcga gctccgccgc cttgaagcgc gtgttcaccg gcacggtgac
    10081 ggcgccgagc agcgcggcgc catagaacag cgcgagccag tgctcgtcgt tgcccatcag
    10141 cagcgcgacg tggtcgccct tccccaggcc gagcgcctgc aggctgccgg cggcctgccg
    10201 cgcgcgcgcg aggagcccgc cgtaggtcag gcgcgccgtg gcggtgacca gcgccgcggc
    10261 cgccggatcc tgcgccgcga ggacttcgcc gagcgtgcgc gggatccagg cgctcaccgc
    10321 ctgcccatcc agtcgccggc ggcgagctgc tcgtcgatcg ccgcgatctc ggttgccagc
    10381 gcgccctgcg ggtcgagctc cacgccgcgg tcgatcgagc gcttggcgag gcgcagcgcc
    10441 tgcgccggcg ccccgacgat ggttttcgcg atcccggcga tgacggcgtc gagcttttcg
    10501 ggcgccacca gcctcgacac cagcccgtgg cgctcggcct cctccgccgt gagcgtgcgg
    10561 ccggtgaaca tcaggtcctt cgccagccgc ttgccgagga tgcgcggcag gcgctgcgtc
    10621 gcgcccaccg tgccgcgcag cgcctccgga atgcggaacg aggccgcggg cgtggcgacg
    10681 atgaagtcgc aggccgccgc gatctcgcag ccggaaccga ccaccggacc ttcgatcacc
    10741 gcgacggcgg gcatcgccag cgcttcgagg gcggcgtagg cggcaaaggc cttcatgcgg
    10801 cgctcgcgta cctgcgccgc gtccatgccc tggcgctcct tcaggtccgc gccggcgcag
    10861 aacaccgggc cgttcgccct gacgaggacc agccggaccg aggcgtccat gccctgcgcc
    10921 gccgcgcgca gggcctcgca cagcgcgagg tcgagcgcgt tgcggctccc gggccggtcg
    10981 agggtgagcg tcgcgacgcc gtccttcgtc tcgcagagga gcgtcatatc gccttcgcct
    11041 cgcgcagggc cgcgatgcgc tcgtcgctgt agcccaggcg ctccttgagg atcgccgccg
    11101 tgtgctcgcc gagggccggc ggacgcccga tctgcggatc gtcccagccg aggaacttgt
    11161 agggcagcgc gagcgccttg aattccccca cgccggggta gtcgaagctg cccaccatcc
    11221 tgcgcgcgcg cacctgggga tcggcgagca cctcggcgac gtcgttcacc ggccccaccg
    11281 gcacgtcggc ggcatcgagc ttcgcgatca gctccgcgcg cgtgagcttc tcgacagccc
    11341 gggtgagctc ggtcatgacc tcgccgcggc gcgcaacgcg cgccgcgttg gcggaaaaaa
    11401 tggggtcaga ccccattttt tccaatccaa gcgcgtcgca cagcggctgc cagtgctggt
    11461 cgctcgccgt gatgtgcgcg tacttgccgt ccttgcagcg gaaggtggcc gaaggcacgc
    11521 ggcccggatg ctcggtgccc aggcgcggcg gcacttcgcc gagggcgaag tagcgtgcgg
    11581 cggccagcgt gagcagcgac acctgcccgt cgagcatgga gaaatccacc tgcgcgccgc
    11641 gtccggtccg ctcgcggccg gagagcgccg caaggatcgc gatcgccacc cacagccccg
    11701 agctgaggtc cgccaccggc aatcccggct tcaccggccc gccgcccttt tcgccggtga
    11761 ggctcatcag tccgcccatc gcctggaaca cggtgtcgta gccctttttc tgcgcgtagg
    11821 gcccggtctg gccgaagccg gtgcaggaga cgtacaccag cttcggattg agcgccgaga
    11881 gcgtggcgta gtccaggccc tgccgtccca gcgtgccgac cgggaaattc tcgacgagca
    11941 cgtcggcctg ctgcgcgagc ccgcggatga tgcggcggcc ttcgtccgag cgcagattga
    12001 cggtgatcga cgccttcgag cggttgcagg cgaagtagta ggcgctgtcc cgcccgagct
    12061 gcggctcgaa gccgcgcgtc tcgtcgcccg cccccggctg ctcgaccttg atcaccgcgg
    12121 cgcccagctc ggcgaggatc atgctcgcga acggacaggc gagcacgcgc gacaggtcga
    12181 ggatcgtcag gtgctcgagc ggcttcatct cagcgccagg cgaggaaggc gatgccggcg
    12241 aggaccagcg cggcgccgag cacggtgcgg gcattgagcg cctcgcggtt cttcagcagc
    12301 cacacgctca ccgggaagat gacgaccggc gtggtgtgca ccaccagcac cgcgagcgcg
    12361 atctcgagcc gcgtcatggc gagcgagatg aagatcgagc cgagcgaggt gaacaccccg
    12421 cagccggtgt agagcaggac ggccttgcgg tcggcggcgc gcagctgcgc ggcgatcccc
    12481 gcccagtccc gcgtcaccac gaactggcag gcgagcgccg ccagcgaggc gaccacggtg
    12541 ccgagcgccg cttcgtccca gacgcgcatg gcgaggccgc gcagcacgtt gccgaagctg
    12601 aagcccgcca cggtgagcag gccgacgagc atccccttgc gcagcatctc cggcgacagg
    12661 gcctgcaccg acccggcgcg cggctgggtg agccacagcc cgaaccacac cagcgccatc
    12721 agcgcgatct cgtaggcgcc gaggacctcg tcggccagca gccaggccgc caccagcgcg
    12781 aatgccggcg ccgtcgaatg gaacacgctc gcgcgcgagg gcccgaggat ccgcaccgcg
    12841 tcgaacagga acctgcggcc gaggaaggtg ccgatcacgc cgccgatggc gaacagcccc
    12901 gcccccttcc agttccaggc gtagggcgcc gcgcgcaccg ccagctcggc tgcgaacagg
    12961 gccgccggaa acagcacatt ggtcgccagc acgatgaaga atcccgcctc caccgggagc
    13021 cgcgccaccg cgtagcgggt gatcatgatg ttggtggcga agcacagcat cgccagcagc
    13081 gcgtaggtga tgcccatggc tcagggcttg cggaatgacc gcatcctctt gttggcttcg
    13141 cgcccgcgtt cgagcgccgc ggcgagcggc aggtccgccg cctcgtggaa cagctctttg
    13201 gtgagccgca tcgccgggcg gctcgcggca gcgagcttct gcgccagcgc gagcgcctcg
    13261 tcgagcaggc gctccggcgg cacgacgcgg ttcaccaggc ccagcgccag cgcgcgctgc
    13321 gcgccgaccg gctcgccgag gccgaccagc tcgaaggccg ccttgcgccc ggcctggcgc
    13381 acgaggttcg ccatcacgat cgccgccacg atgccgtgcc tggtctccgg gtagccgagc
    13441 gtcgcgccct cggccatcac cgccaggtcg ccggcgatgg cgaggcccgc gccgccgccc
    13501 atcgccgcgc cgttgatcgc ggtgaccacg ggcttcgcca tcccggggaa ggcgaggtgc
    13561 agctgcatgc tgagttccgc gcgcgcctcg acgagcggct ggttcccggg cgtgagttcc
    13621 ttgaattcgg cgaggtccgc gccggcgcaa aagccctgcc ccgcgccggt cagcaccacg
    13681 cagcccactg cctcgtccgc atccgccgcg cgcagcgcat cgagcagcgc ctgcgtcaac
    13741 gcggtgttca gcgcgttccg cttctccggg cgattgagcg tgaggacgcg caccgcgccc
    13801 tggtcccgga cgatgatcac agggccgcga ggcgcttgcg cgcctcctcc agttcgacca
    13861 tcaggcgctt caccacttcg gcggcgggca ggatctcgtt caccaggccg gcggactgcc
    13921 ccgcctcgac cttgccccat tcgacgtcgc cgtcgagcgc cgcctgcttg agggtggccg
    13981 ccctgaacag cgcgtcgagc tcggcctgcg gcgcgccggc gcgctccgcg gcgtcgaatt
    14041 tcgcggtgaa cgcgttcttc agctgccgca ccggcagctt gccgcgcgcg acgagcgtcg
    14101 agccgtccac gtcggcggcg agcaccgcgc gcttgtagtt gtcgtgcacg ctcgcctccg
    14161 gcgtggcgat gaagcgcgtg ccgagctgca ccgcgtccgc gccgagcgcg aggagcgccg
    14221 cgatgccggc gccgtcggcc acgccgccgc tcgccaccac cggcagcttc gtcgccttca
    14281 ccacgcggcg caccagcacc agggtgctga cctcggaggg cggcgggtgc ccgcccgcct
    14341 ccatgccgtc caccaccagg gcgtccacgc cggcggcttc ggccttccgc gcgtgctcga
    14401 ccgaggcgac gacgtgcagc cacctggcgc cgatggcgcg aaagcgcgcg agctgctcct
    14461 tcggcccgcc ctgcgaggcc accatcacgg gaatgcgcgc ttcgaacgcg atgtcgagca
    14521 ggtcggcgga gcgcggattg ttgagcggga tattgacgcc gtagggctgg tcggtgcccg
    14581 acttgatggc ggcgagcgag cgccggaatt cgtcgttgcg catcggcccc gcggcgatca
    14641 cgccgagcgc cccggcgttg gacaccgcga tcaccagcgg cgcgctggag gaggcccagc
    14701 tcatcgccgc ctggaacacc gggtggcgga tgccgagcag ctcggtgatg cgcgtcttca
    14761 ccgcggcgcg ccctcgcgct cgatgaaggc gcgcgcgcgc gcggcgagct tgccgagcgc
    14821 ggcgtcgaag gcggcgcgct ccttcgcgga aaggcaatcg aggaaggcgt cgttccgctc
    14881 cgccgcggcc cgcatcagcc cctggtacag cgcgcgcccg gcgcgcgtca gcgtgagccg
    14941 cacgccgcgc ccgtcctcgg cgtcgatggc gcgcagcacc aggcgccgcg cggcgaggcc
    15001 cgacaccacg cggctcatct ggctcttgtc gatgcccgcg gcgcgcgcga ggtcgttgag
    15061 ggactgcgcg cccgcggcgc cgccgagcag cgccacgctg cgccactccc acaggctgac
    15121 gccgaactcg cgccggtagc gcagctccgc gccgcgcgac aggagattgg cgaccacgtg
    15181 cagccggtac gacagcaggt cccggatgcc gggcgcgtca atcgtcgaag aacggggttt
    15241 catgctcgcc gcgcagcttg aggtcgaagc ggtagcgtcc cggcgcttcc tcgcgcgcca
    15301 ccagcagcgc gcgccgcgcc tcgggcaccg cctggtagac gggatccttc ccgttcgcct
    15361 gctcgccggg aaaaaacatc gtggtcttca ggatgcgcat cagcccggag aacatcacca
    15421 tgaagttcac gtgccgcgcg cggcccgcgg gcgcgcccgg gcgcacgctg cggaaggcgt
    15481 agttgcccgc cttgtcggtg gccgcgcggc cccagccgag gaaatccgcg tcggcctcgc
    15541 ggtggcgcgg gtcggcgcga tggcggtaga tgccgttcgc gtccgcctgc cagatctcga
    15601 gcagcaggtt gtccagcggc gcgccgtcct cctggcgcac gcagccgacg atctcgatcg
    15661 cctctccccc ggccttgcgg cccccgcatt gcgtgaggtc gttggccccc gcgtccacgt
    15721 agcgcggcgg gaagaacggc ccgagggtgg cttctccggt gggaatcacg gctggaccgg
    15781 ggtcgcctgg ctgccgcgca gcacgatctg gtgctcgaac acgagcgccc cgtgcggctc
    15841 gccggccggc ggcaccaggc gcgcgatcag ccgctcgcgc gccgcggcat ccggcacggc
    15901 gttcaggatg cggtcctggg gattgagcgg ctcgcccggg aagtacatct gcgtcaccag
    15961 gcgcgacagc cacaccttgc cgaagagcga aaaatggatg tgcggcggcc gccaccagcc
    16021 gtccttcgtc ggcaccgggt aggcgcccgg cttcacggtg cgcaaggtga aggcgcccga
    16081 ttcgtccgtc accaggcgcg ccgcgccgta gaagttgcgg tccaccggcg catgctcgtc
    16141 gtcgttcggg tggatgtagc gccccgcggc gttgcattgc cagatctcga ccactgcgcc
    16201 gccgaccggc gcgccgtcct cgtccaccac gcgcacccgc aggtgcatca gctggccgat
    16261 cgcctgcgtc tcgccgcggc ccgcgatgtc gccgccgagg agctgcacgc gcttctcgac
    16321 gcccgccggg ccggtgagct cggagagcgt cggcgcgcgc ctgaccagcg gctcccgcgg
    16381 gttgcgccag ggcgtctgcg ggtagcccgg gacgatgcgc gccggctgcg tgcgctcgtc
    16441 gcgcggggcg atccggtagt ccatcgttga cagcctagcg ccggatagtt gatgatgtca
    16501 actatggccg acgtgacgct gtgcgagtgc ttcgcgcgcg acgggctgca gcacgagccc
    16561 gccttcatcg ccacggacgt gaagcgcgcg ctcgtcgagc gcttcgccgc gctcggcttc
    16621 cgcaggatcg aggccacctc ctactccaac ccgaaagtcg tgccccagtt cgccgacgcg
    16681 agcgagctgc tgcgctcgct gccgcggcgc gacggcgcgc attacaaggc cacctgcgcc
    16741 aacccgaagg cggtggagcg cgccctcgcc gacctcgacg ccgggttcgg cgtcaacgag
    16801 atcagcctgc tggtctcggc caccgaggcg catacgcaga agaacctgaa gcgcacgcgc
    16861 gccgggcagt gggcgaacgt ggaacagatg gtgcagctcg ccggcgggcg cttccggctg
    16921 gtgggcacgg tgtcggtggc cttcggctgc cccttcgagg gcaaggtgga ccccggcgtg
    16981 gtggcggcgg acgtggcgaa gttcgtctcc ctcggcgtcg cgcacgtcgc gctgggcgac
    17041 accacgggcc tcgccacgcc gacgtccgtg aaggacatgg tgcggcgcat gcccgcgggc
    17101 gccattccca tcgcccactt ccacgacacg cgcggcacgg ggctcgccaa ctatgtggcg
    17161 gcgcatgaag caggcgtgcg ctttttcgat gcctccttcg gcggcgtggg cggacatccg
    17221 gcgaaggtga agtacggcgg gggccacacc ggcaacgtgt gcaccgagga cctggtcaac
    17281 cttttcgaga cgatgggcat cgataccggc atcgatctgg acaagctgct cgctgccgcc
    17341 gaatattgcg agcaggtgct tggccgtgag ctgcacggcc gcgtgacccg gagcggactc
    17401 ggccccagga gatgaagccc ccttatcgcg ccgaccaggt cggcagcctg ctgcgccccg
    17461 cggagctttc cgcggcgcgc aaggagtgga agcagggtgc cctcgccgcc gatgcgctga
    17521 aggtgatcga ggatcgcgcc atccgcgagg cagtggcgaa gcaggaggcg ctcggcctgc
    17581 aggcggtcac cgacggcgaa ttccgccgcg actggtggca cctcgacttc ctcgcccagc
    17641 tcgatggcgt caccacgcgc gagaacccgg gtccgaagtt cggcggcacc gaggagcagc
    17701 cgccgatcgc caccgtcacc ggcaaggtcg gctgctccag gccgatcatg gtggagcact
    17761 tcgcgttcct caagtcggtg gcgaaggcga cgccgaagtt caccatcccc tcgccgtcga
    17821 tgttgcacct gcggggcgga cgcaatgcca tctccaggga cgcctacccg gacctggagg
    17881 atttctggga ggacgtc
//
LOCUS       DASRIE010000086         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2715313, whole genome shotgun sequence.
ACCESSION   DASRIE010000086 DASRIE010000000
VERSION     DASRIE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715313"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFB53_04020"
     CDS             <1..714
                     /locus_tag="VFB53_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZN85934.1"
                     /translation="IYRASSIKVAEAAKVIENTQRDLNIAFVNELSIIFERMGLDTLE
                     VLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAELLGYHPQVILAGRRINDGMGPH
                     VARKAIQQMIHAGRNIKGARVNVLGLTFKEDIPDIRNSKVADILHELREFGVEAYVHD
                     PMASAEDALHEYGVKLRSWEELPAADALILAVPHKRYLEMPAHNLIKKVVRGGCVIDV
                     KSVLDAQAFRREGLRVWRL"
     gene            702..2270
                     /locus_tag="VFB53_04025"
     CDS             702..2270
                     /locus_tag="VFB53_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85935.1"
                     /translation="MAAVALPAAAAARPAAQPAAHPAAGKLRVAVFADARLQPRWVVD
                     AFTAIARSECAEVVAVGAGTGSGNTESWLTRAYAGLDQWAFGGDGRQPVDLVEALADA
                     RLFTPPFPGLLELDLDVVFALGEIDEAPFHGVARLGVWRFQADGAAEVAAGEPLSGSA
                     LTVRLAPGAPPRIAYQSWSRTDPLSISRNRAALLRKTAEFPLRALREARRSGREWLEQ
                     RPLMRNGIHEKRGLSLISIGGRIIKRSVEKAVAIEQWSLAFSFERRDLNPTLEGFKRI
                     VPPADRDWSEPFALERNGRYYVFFKEMLRGAGKAHIAMLELDPDGRWSAPVRVLERDY
                     HLSHPFVLEHDGQIYLVPESAKNRTVEVYRCVDFPRRWRLERVLLDGVRLVDATFLRA
                     PERLWMFANGAAGDSGIFNDELYLFHAASLLDEWQPHARNPVRSDVRGARPAGALYWR
                     GGALYRPAKNCVPRYGAGVTLNRVLRLSPQAYAERPVGQILPPPGGALLGVHTVNRAG
                     RLTVADALARRRRL"
     gene            2348..3499
                     /locus_tag="VFB53_04030"
     CDS             2348..3499
                     /locus_tag="VFB53_04030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN85936.1"
                     /translation="MSLSNPLPFFVICALCLLIGWALARSSAFYRAQVDVAGRFESID
                     GLRGFLALGVFAVHAVNMYTLLSYGGWSSDIAPFHNKAASAGVALFFMITGFLFWLRV
                     LRAGSAFDTRAFFASRLWRLTPMYLMSVVMVVAVVAAATGFALHASPATLARELRPWL
                     AFGFMDTGSVNGLADARYINAVYWTLAYEWLFYLALPLLALFARGWGAAALVLLVLAF
                     GTQKPIVFNFLFGAFAAWIVHRRLLEGRLSSPWLGVLPLASLGAYFALPTGNGLPQSA
                     ALFGFFIFVVHGWSLFGLLRTRAAKVLGTISYSIYLTHCIALYVTMHAVDRLVPVRTL
                     EGGEYWMLAGLAAAGCVLLSALTYRYVEHPFLHSQPARKPARAHAEAVV"
     gene            3532..4602
                     /locus_tag="VFB53_04035"
     CDS             3532..4602
                     /locus_tag="VFB53_04035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN85937.1"
                     /translation="MLGAEPETRGSIAAVVDAFRSHGLFQRWPIDYLPIHGGRGPANN
                     AALAFGAARRFALLLARHRRALVHMHVCARGSALRDAAFMAAALAVRCPLIVQLHGAG
                     LERSHGNALIGQLLASAACVLIPCESLRPWVRSVARGAHTECLPAPVACAAPSSHETR
                     PNLILFLGRLEAAKGIFDLLEAVAAVRATIPDVRLVCAGDGERIAVARYAERLGIADA
                     VKFTGGVGPSGKRALLETAAVFALPSYDEALPVGLCEAMAAGVPPVVSPVGGIPELVT
                     DGVSGCLVAPGDKAGLERNLRRLLADRALGARLGAAARETARMRFAPERTLGRLEEIY
                     AALGLQSAPEAMARPADLRQAA"
     gene            4602..>5187
                     /locus_tag="VFB53_04040"
     CDS             4602..>5187
                     /locus_tag="VFB53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85938.1"
                     /translation="MGGIAGWITPARNAPDPAAIVPALEALAHRGASGEGIYVAHSES
                     GHQVVLAQPYCDDADNAVALDGTIANHIDLKTQLALRGFAPAEGNTRVLLDAYEQWDK
                     DVVHHLRGAFAFALWDAGRERLLLARDRFGEKPLYLCEREGALYFASEIRALLKMPGV
                     AALADPRAVHDYLAYRYVPAPRTLISGVRKLMPGT"
BASE COUNT          657 a         1906 c         1823 g          801 t
ORIGIN      
        1 atctaccgcg cgagctcgat caaggtggcc gaggccgcca aggtgatcga gaacacgcag
       61 cgcgacctga acatcgcgtt cgtcaacgag ctgtcgatca tcttcgagcg catgggcctc
      121 gacacgctgg aagtgctcag ggcggcgggc accaagtgga acttcctgcc gttccggccc
      181 ggcctggtcg gcggccactg catcggcgtc gacccttact acctcacgca caaggccgag
      241 ctgctcggct accacccgca ggtgatcctc gccgggcggc gcatcaacga cggcatgggg
      301 ccgcacgtcg cgcgcaaggc gatccagcag atgatccacg ccggccgcaa catcaagggc
      361 gcgcgcgtca acgtgctcgg cctcaccttc aaggaagaca tcccggacat ccgcaactcg
      421 aaggtggccg acatcctgca cgagctgcgc gagttcggcg tcgaggcata cgtgcacgac
      481 ccgatggcga gcgcggaaga cgcgctgcac gagtacggcg tcaagctgcg ctcgtgggag
      541 gagctgcccg cggccgacgc cctgatcctc gcggtgccgc acaagcgcta cctcgagatg
      601 ccggcgcaca acctgatcaa gaaggtggtg cgcggcggct gcgtgatcga cgtcaagtcg
      661 gtactcgacg cgcaggcgtt ccggcgcgag gggctgcggg tttggcggct gtagcgcttc
      721 ccgctgccgc cgccgcccgc cccgcagcgc agcccgccgc gcatcccgcg gcggggaagc
      781 tgcgcgtcgc cgtgttcgcc gacgcgcgcc tgcagccgcg ctgggtggtg gatgcgttca
      841 ccgccatcgc gcgctcggaa tgcgccgaag tggtggcggt gggcgccggc acgggctccg
      901 ggaacacgga atcctggttg acccgcgctt acgcaggcct cgaccagtgg gccttcggcg
      961 gcgacggccg ccagccggtg gacctggtgg aggcgctggc ggacgcgcgc ctgttcacgc
     1021 ccccgttccc cggcctgctc gagctcgacc tggacgtggt gttcgccctc ggcgaaatcg
     1081 acgaggcgcc gttccacggc gtggcgcggc tcggcgtgtg gcgcttccag gccgacggcg
     1141 cggccgaggt cgccgccggc gagccgctct ccggctcggc gctcaccgtg cgcctcgcgc
     1201 ccggcgcgcc gccgcgcatc gcctaccagt cgtggtcgcg caccgacccg ctctcgatct
     1261 cgcgcaaccg cgcggcactg ctgcgcaaga ccgccgagtt cccgctgcgc gcgctgcgcg
     1321 aggcgcggcg ctcgggccgc gaatggctcg agcagcggcc tttgatgagg aatggaatcc
     1381 acgaaaaacg tggtctgtcc ctgatttcga tcggcggccg catcatcaaa cgcagcgtcg
     1441 aaaaagccgt ggcgatcgag caatggtcgc tcgcgttctc cttcgagcgg cgcgacctga
     1501 acccgacgct cgagggcttc aagcgcatcg tgccgccggc cgaccgcgac tggtcggagc
     1561 cgttcgcgct ggaaaggaac ggccgctact acgtgttttt caaggagatg ctgcgcggcg
     1621 cgggcaaggc gcacatcgcc atgctcgagc tcgaccccga cggccgctgg tcggcgccgg
     1681 tgcgcgtgct ggagcgcgac taccacctgt cgcatccgtt cgtgctcgag cacgacggcc
     1741 agatctacct ggtgccggag agcgcgaaaa accgcaccgt cgaggtctac cgctgcgtcg
     1801 atttcccgcg ccgctggcgc ctggagcgcg tgctgctcga cggcgtgcgc ctggtggacg
     1861 ccaccttcct gcgcgccccc gagcggctgt ggatgttcgc caacggcgcc gccggcgaca
     1921 gcgggatctt caacgacgag ctgtacctct tccacgccgc ctcgctgctg gacgagtggc
     1981 agccccacgc gcgcaacccg gtgcgctccg acgtgcgcgg cgcgcgaccg gcgggcgcgc
     2041 tctactggcg cggcggcgcg ctctatcgcc cggcgaagaa ctgcgtgccg cgctacggcg
     2101 ccggcgtgac cctgaaccgc gtgctgcgcc tgtcgccgca agcctacgcc gagcggccgg
     2161 tcgggcagat cttgccgccg ccgggcggcg ccttgctcgg cgtgcacacg gtgaaccgcg
     2221 ccggccggct cacggtcgcg gatgcgctcg cgcgccgccg gcggctttga gccgttccgc
     2281 ctaggccggc gatcacagcg ccatcacgcc ggctgcccat aatcccctgc aggtaatgct
     2341 tgaagccatg agcctttcca acccgctccc gttcttcgtg atctgcgcgc tgtgcctgct
     2401 gatcggctgg gcgctcgcgc gctcgagcgc cttctaccgc gcgcaggtgg acgtggccgg
     2461 ccgcttcgag agcatcgacg gcctgcgcgg cttcctggcg ctcggcgtgt tcgcggtgca
     2521 cgcggtcaac atgtacacgc tgctcagcta cggcggctgg tcgagcgaca tcgcgccgtt
     2581 ccacaacaag gcggcgagcg ccggcgtggc gctgttcttc atgatcaccg gattcctgtt
     2641 ctggctgcgc gtgctgcgcg ccggcagcgc gttcgacacg cgcgcgttct tcgcctcgcg
     2701 gctgtggcgc ctgacgccga tgtacctgat gtcggtggtg atggtggtcg cggtagtcgc
     2761 cgccgccacc ggcttcgcct tgcacgcgag cccggcgacg ctggcgcgcg agctgcgccc
     2821 ctggctcgcg ttcggcttca tggacaccgg gtcggtgaac ggcctggccg acgcgcgcta
     2881 catcaacgcg gtgtactgga cgctggccta cgagtggctg ttctacctcg cgctgccgct
     2941 gctcgcgctg ttcgcgcgcg gctggggcgc cgcggcgctg gtgctgctgg tgctcgcctt
     3001 cggcacgcag aagccgatcg tgttcaattt cctgttcggc gcgttcgccg cctggatcgt
     3061 gcaccggcgc ctgctcgagg gacgcctctc ctcgccgtgg ctcggcgtgc tgccgctcgc
     3121 gtcgctcggc gcctatttcg ccctgcccac cggcaacggc ctgccgcaga gcgcggcgct
     3181 gttcggcttc ttcatcttcg tggtgcacgg ctggagcctg ttcgggctgc tgcgcacgcg
     3241 cgccgccaag gtgctcggca ccatcagcta cagcatctac cttacgcatt gcatcgcgct
     3301 ctacgtgacg atgcacgcgg tcgaccgcct ggtgccggtg cgcacgctcg aaggcggcga
     3361 gtactggatg ctcgccgggc tggcggccgc gggctgcgtg ctgctctcgg cgctgaccta
     3421 ccgctacgtc gagcacccgt tcctgcactc ccaaccggcc cgcaagcctg cgcgggcgca
     3481 cgccgaggcg gtggtatgaa gccagagtcg tcttcctttc ctcgcttgat catgctgggc
     3541 gcggagcccg agacccgcgg cagcatcgcc gcggtggtcg atgcctttcg cagccacggg
     3601 ctgttccagc gctggccgat cgattacctg ccgatccacg gcggccgcgg cccggcaaac
     3661 aacgccgcgc tcgccttcgg cgccgcgcgc cgcttcgcgc tcttgctcgc gcgccaccgc
     3721 cgcgcgctgg tccacatgca tgtgtgcgcg cgcggcagcg cgctgcgcga cgcggccttc
     3781 atggccgcgg cgctcgccgt gcgctgtccg ctcatcgtgc agctgcatgg cgccgggctc
     3841 gagcgctcgc acggcaacgc gctcatcggg cagctgctcg cgagcgcggc ctgcgtgctg
     3901 atcccgtgcg aatcgctgcg cccctgggtg cgcagcgtgg cgcgcggcgc gcacacggaa
     3961 tgcctgccgg cgccggtcgc ttgcgcggcg ccgtcgtcac acgagacgcg gccgaacctg
     4021 atcctgttcc tcggccggct cgaggcggcg aagggcattt tcgacctgct cgaggcggtg
     4081 gcggcggtgc gcgccaccat cccggacgtg cgcctggtgt gcgcgggcga cggcgagcgc
     4141 atcgcggtcg cgcgctacgc cgagcgcctg ggcatcgccg acgcggtcaa gttcaccggc
     4201 ggggtcgggc cctccggcaa gcgcgcgctg ctcgaaaccg cggcggtgtt cgcgctgccg
     4261 tcctacgacg aggcgctgcc ggtggggctg tgcgaggcga tggccgccgg cgtgccgccg
     4321 gtggtttcgc cggtcggcgg catcccggag ctcgtgaccg acggcgtgag cggctgcctg
     4381 gtcgcgcccg gcgacaaggc cggcctcgag cgcaacctgc gccggctgct ggccgaccgc
     4441 gcgctcggcg cgcgcctcgg cgccgccgcg cgcgagaccg cgcgcatgcg cttcgcgccc
     4501 gagcgcacgc tcgggcggct ggaagaaatc tacgccgcgc tcgggctgca gtcggcgccc
     4561 gaggcgatgg cgcgtcccgc cgacctcagg caggcggcct gatgggcggc atcgccggct
     4621 ggatcacccc ggcgcgcaac gcgcccgacc cggccgcgat cgtgccggcg ctcgaggcgc
     4681 tcgcccaccg cggcgcgtcg ggcgagggca tctacgtcgc gcactcggag agcgggcacc
     4741 aggtggtgct ggcgcagccc tactgcgacg acgccgacaa cgccgtcgcg ctcgacggca
     4801 ccatcgccaa ccacatcgac ctgaaaacgc agctggcgct gaggggcttt gcccccgcgg
     4861 agggcaacac gcgggtcctg ctcgacgcct acgagcaatg ggacaaggac gtcgtccacc
     4921 acctgcgcgg cgcctttgcg tttgcgctgt gggacgccgg gcgcgagcgc ctgctgctcg
     4981 cgcgcgaccg cttcggcgag aagccgctct acctttgcga gcgcgagggc gcgctctact
     5041 tcgcctccga gatccgcgca ctgctcaaga tgccgggcgt ggccgcgctc gccgacccgc
     5101 gcgcggtgca cgattacctc gcttaccgct acgtgcccgc gccgcgcacc ctgatcagcg
     5161 gcgtgcgcaa gctgatgccg ggcacct
//
LOCUS       DASRIE010000087         8271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3759902, whole genome shotgun sequence.
ACCESSION   DASRIE010000087 DASRIE010000000
VERSION     DASRIE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3759902"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFB53_04045"
     CDS             complement(<1..172)
                     /locus_tag="VFB53_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85939.1"
                     /translation="MPTRHHRLIILGSGPAGYTAAVYAARANLEPMLITGMAQGGQLM
                     TTTEVDNWPADVH"
     gene            365..2446
                     /locus_tag="VFB53_04050"
     CDS             365..2446
                     /locus_tag="VFB53_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HZN85940.1"
                     /translation="MAATLCLAAIPAGAAGLGQLAVQSHLGEPLRAHIEIVGLQPGEE
                     ATVRIAPRAVHIRAGIEYPALLGELRVALERRDGVTVASISGTQPVDLPAINFIVELT
                     SASGSLMRQYALLPSPAPRAAGRAPLSPAPLSPVPPAPVVVEAPPKAAEAPRPQEDSP
                     RFAITRYLFDGAALVPLERLQEATAPYTGPGRSFGDVQRALEAIERAYAATGYSAVQV
                     LLPEQELDRGEVRFRVLEAKLGRVLVEGNRHFDQANVRASVPALVPGEAPNILAVGRN
                     LRVANENPAKQATVLLRSGREEGTVDAAVRVVDGPAQRYSLTLDTSGTRETGRLRLGV
                     GYQNANTAGSDHVLTLQYVGAPHDKNHPSRISLIPSSRVFVIGAGLRIPLYEEGDAID
                     LTAGYSNVDSGTVGELFNISGAGGLFGARYTHYLDRQGDYDSRLSLAWDHRGYHFKGI
                     RAVGSNQQLQPDIAVRPITLAYQGQWRQPDGETSFSLAVSRNVPGGNDGRDEDFCQLG
                     PNPPHGFSRSDGMGNCPDPNYVAWRWSVTHLSALPGNWQLRAGVNGQYTRDMLIAGEQ
                     FGLGGADSVRGFEERALSDDRGWRGTLELYTPDFGGSTGIAGARARGLVFYDVGGLRR
                     IRPAPGDALTQSVSSAGIGLRLTRGSSLSFRMDWGWVIDPGGSPGLNGDPGTFQKRGD
                     GRLHASLSYVF"
     gene            2488..3156
                     /locus_tag="VFB53_04055"
     CDS             2488..3156
                     /locus_tag="VFB53_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZN85941.1"
                     /translation="MAVAVSSAMLRSVPLFASFPEEPLRLLAAVVTRRSATRGSIIMA
                     AGDPTDSLYIVLSGRLKVMMSDADGKEVILSILGPGEFFGEMGLIDDSPRSASVVAIE
                     PCELMAITRRDFKKCLAENFDMASAVMRGLVRRLREADRKIGSLALLDVYGRVARLLL
                     DMAETVDGQKMVTKRLPKQDIAKMIGASREMVSRVMKDLQIGGYIEMRGSTIVLRDTI
                     LLPD"
     gene            3243..3950
                     /locus_tag="VFB53_04060"
     CDS             3243..3950
                     /locus_tag="VFB53_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZN85942.1"
                     /translation="MALNRTFVTLLCALALAPAWADSGSVAQLSGTLSVKKPDGSVRI
                     LARNSEVRSGDVLSTERDSYAQIRFSDGGQITLKPGTAVKLDDFKYSAEKPQEDAFLF
                     SLLRGGLRAVTGLVGKRNRDRYLMNTATATVGIRGTTFSADDCTGTRDGECARLQQAV
                     YVGVSDGEIVVRNAQGELALGAGQFGHIARGEKPLFLSTDPGLQFAPPATFIQSLLSG
                     RQVAGIGEKLECAISAR"
     gene            4007..6286
                     /locus_tag="VFB53_04065"
     CDS             4007..6286
                     /locus_tag="VFB53_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZN85943.1"
                     /translation="MAAAQKTTAAPLPAKLANLLREARWLVLASIAVYLLLIFLTFHR
                     GDPGWSLSATAADVHNAGGRAGAWLSNLLLYLFGVSAYWWIALCLVGVAWGYRSLDRS
                     ALIDRRPLAIALFGFALLLLSSAALESLRLHSLPAQLPLAPGGLLGAEIGGFAAGLLG
                     FTGATLLLLTLAAVGFSLFTGVSWLAVAELTGLLLEAGAGLARSTWERRADRKAGEFA
                     REEREVVVEAEKRREEDHPPLVIAPPVVEIRKSERVQKEKQAPLFDHLPDTPLPPVKL
                     LDEAAQDGAQVSAESLEFTSRLIEKKLSDFGVSAKVLAAYPGPVITRYEIEPAVGVKG
                     SQIMNLVKDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQTVRLSEILGSELYAGMH
                     TPLALALGKDIAGNAVVVDLQKMPHLLVAGTTGSGKSVAINAMILSLLYKSEPKQVRM
                     IMVDPKMLELSMYQDIPHLLAPVVTDMKQAANALMWCVAEMERRYKLMSWVGVRNLSG
                     FNHKVADAEKSGRPLEDPATLESGAPQPLGQLPYIVVVIDELADLMMVVGKKVEELIA
                     RLAQKARASGIHLILATQRPSVDVITGLIKANIPTRIAFQVASRIDSRTILDQQGAEA
                     LLGQGDMLYLPAGTGLSQRVHGAFVADHEVHKVVEHLKSLAAPEYIEDILDPAIGEEP
                     GGNGANGEPGGEKDPLYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIEDMERAGL
                     VSAMQTNGNREVLVPAKAE"
     gene            6288..6893
                     /gene="lolA"
                     /locus_tag="VFB53_04070"
     CDS             6288..6893
                     /gene="lolA"
                     /locus_tag="VFB53_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZN85944.1"
                     /translation="MRAVLAVALCVPLFACAAGVERFRDYLRSTQTARADFEQRVFDR
                     EKKLVQHSRGSFSLQRPGRFRWTYAQPYPQLIVGDGARVWIYDEDLNQVTVRAMAKAL
                     GSTPAALLAGAAEVERAFALKDAGRRDGLEWVECTPREKEAGFERILLGMGASGVQAM
                     ELADHFGQTTVLRFSNIARNPALDAAAFRFTPPKGADVLGD"
     gene            6877..8178
                     /locus_tag="VFB53_04075"
     CDS             6877..8178
                     /locus_tag="VFB53_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZN85945.1"
                     /translation="MSSATDLFPAAAPAAPLAERLRPRSIDEVLGQKHLLGPGRPLRL
                     AFESGKPHSMILWGPPGTGKTTLARLMAQAFDAEFIALSAVLSGVKEIRDAVAHAEAV
                     IAASGRRTILFVDEVHRFNKAQQDAFLPYVEKGLFTFIGATTENPSFEVNSALLSRAT
                     VYVLESLPPEDLGALIDKAGLATDAEARARLVGFADGDARRLLNAVEILQNARPRGAI
                     DAAFLESTLAKNLRRFDKGGEQFYDQISALHKAVRGSDPDASLYWLVRMLDGGADPLY
                     LARRIVRMAVEDVGLADPRALTLALEAAQTYERLGSPEGELALAEAVIYMACAAKSNA
                     VYNAYNAARAFVGEDGTRPVPLHIRNAPTKLMKNLGYGKDYRYAHDEPDAFAAGENYL
                     PEGMPAVAWYKPTERGLEGRIREKLEELKRLNAVARGKKNK"
BASE COUNT         1251 a         2856 c         2952 g         1212 t
ORIGIN      
        1 cgtgcacgtc ggccggccag ttgtcgacct cggtcgtggt catcaactgg ccgccttgcg
       61 ccatgccggt gatcagcatc ggctcgagat tggcgcgggc cgcgtagacc gcggcggtgt
      121 agccggcggg gccggaaccc aggattatga ggcggtggtg gcgcgtgggc atcgcgaagg
      181 cgggatgtgc aaggggagcg gggccgcatt ataagttaag cggtccgacg gcggccgggc
      241 gcttcgttgt tcgggaatgc gatctctgtt ctaatctgcg cgcagcgcgt tggcgggaga
      301 gtcgggggga acgttggcat tacatgaggc cgcggtacgg tggctgcaag gggcttgcct
      361 tgctgtggcg gcgacgctat gcctggcggc cattccggcc ggcgcggccg gactcgggca
      421 actggcggtc cagtcccacc tgggcgaacc gctgcgcgcg cacatcgaga tcgtcggcct
      481 gcagccgggc gaggaagcga cggtgcgcat cgcgccgcgc gcggtgcata tccgtgcggg
      541 catcgaatac ccggcgctgc tcggcgaatt gcgcgtggcg ctcgagcgcc gcgacggcgt
      601 gacggtggcc agcatcagcg gcacgcagcc cgtcgacctg ccggcgatca acttcatcgt
      661 cgaactgact tcggcttccg gctcgctgat gcggcaatac gcgctgctgc ccagtccggc
      721 cccgcgggcc gccggccggg cacccctgtc gccggcgccc ctgtcgccgg tgcctccggc
      781 gccggtcgtc gtcgaggcgc cgccgaaggc ggcggaagcg ccgcggcccc aggaagactc
      841 gccgcgcttt gccatcaccc gctacctgtt cgacggggcg gcgctggtgc cgctggagcg
      901 cctgcaggag gccaccgcgc cctacaccgg gcccggccgc agtttcggcg acgtgcagcg
      961 cgcgctcgag gcgatcgagc gcgcgtacgc cgccaccggc tacagcgcgg tgcaggtgct
     1021 cctgcccgag caggagctgg accggggcga agtgcgcttt cgcgtcctcg aggcgaaact
     1081 cggccgcgtg ctggtcgagg gcaaccggca tttcgaccag gcgaacgtcc gcgccagcgt
     1141 gcccgcgctg gtgcccggcg aggcgcccaa catcctcgcc gtcggccgca acctgcgcgt
     1201 ggcgaacgag aatcccgcca agcaggccac cgtgctgctg cgcagcgggc gcgaggaggg
     1261 gacggtggac gcggcggtgc gcgtggtcga cgggccggcg cagcgctaca gcctgacgct
     1321 ggacacgagc ggcacgcgcg agaccggcag gctgcgcctc ggggtgggct accagaacgc
     1381 caacacggcc ggcagcgacc acgtgctgac gctgcagtac gtcggcgcgc cgcacgacaa
     1441 gaaccacccc agcaggattt cgctcatccc cagctcgcgc gtgttcgtca tcggcgccgg
     1501 actgcgcatc ccgctctacg aggagggcga cgcgatcgac ctcaccgccg gctactcgaa
     1561 cgtcgactcc ggcacggtgg gcgagctgtt caacatcagc ggcgcgggcg gacttttcgg
     1621 cgcgcgctac acgcactacc tcgaccggca gggggactac gacagccgcc tgtcgctcgc
     1681 ctgggaccac cgcggctacc atttcaaggg catccgcgcc gtcggcagca accagcagct
     1741 gcagccggac atcgccgtgc ggcccatcac gctcgcctac cagggccagt ggcggcagcc
     1801 cgacggggaa acctcctttt cgctcgcggt gtcgcgcaac gtgcccgggg gcaatgacgg
     1861 ccgcgacgaa gacttctgcc agctcggccc caacccgccg cacggctttt cgcgctccga
     1921 cggcatgggc aactgcccgg accccaacta cgtggcgtgg cgctggtcgg tgacccacct
     1981 cagcgcgctg ccgggcaact ggcaactgcg ggctggcgtg aacgggcagt acacccgcga
     2041 catgctgatc gccggcgagc agttcgggct gggcggcgcg gactcggtgc gcggcttcga
     2101 ggaacgcgcc ctgtccgacg accgcggctg gcgcggcacc ctggagctct acacgcccga
     2161 tttcggcggg agcaccggca tcgccggggc gcgcgcgcgc gggctcgtgt tctacgacgt
     2221 cggcggtctg cgccgcatcc gcccggctcc cggcgacgcg ctcacgcaga gcgtctcgag
     2281 cgccggcatc ggcctgcgcc tcacgcgcgg ttcctccctg tcattccgca tggattgggg
     2341 ctgggtgatc gaccccgggg gcagccctgg cctgaacggc gatccgggca ccttccagaa
     2401 gcgcggcgac ggccggttgc atgccagcct gtcgtacgtc ttctaggcgg gcaactttgt
     2461 taacataggt caaaaggggc cttggcaatg gcggtggcgg tttcctcggc aatgctgcgc
     2521 tcggtgccgc tcttcgcgag cttcccggag gagccgctgc gcctgctggc cgcggtggtg
     2581 acgcggcgca gcgcgacgcg gggctcgatc atcatggccg ccggcgatcc caccgactcg
     2641 ctgtacatcg tgctctccgg ccgcctcaag gtgatgatga gcgacgccga cggcaaggaa
     2701 gtgatcctca gcatcctcgg gccgggggag ttcttcggcg agatgggcct gatcgacgac
     2761 agcccgcgct cggcgagcgt ggtggcgatc gagccctgcg agctgatggc gatcacgcgg
     2821 cgcgacttca agaagtgcct ggcggaaaac ttcgacatgg cctcggcggt gatgcgcggg
     2881 ctggtgcggc gcctgcgcga ggccgaccgc aagatcggca gcctggcgct gctcgacgtc
     2941 tacggccgcg tggcgcggct gctgctggac atggccgaga cggtggacgg gcagaaaatg
     3001 gtcaccaagc gcctgcccaa gcaggacatc gccaagatga tcggcgcctc gcgcgagatg
     3061 gtgagccggg tgatgaagga cctgcagatc ggcggctaca tcgagatgcg cggctcgacc
     3121 attgtgctgc gcgacaccat cctgctgccc gattgaggcg ccgaatgccc gtcaaatcag
     3181 ctgtttaggc gtgacataat tcctcgtcgc cggatgccgc ggcgcctagc atactgaaag
     3241 ccatggccct gaatcgcacc ttcgtgactt tgctctgcgc gctcgcgctg gcaccggcct
     3301 gggccgacag cggctcggtg gcgcagctct cgggcacgct gtcggtgaag aagcccgacg
     3361 gctcggtgcg catcctggcg cgcaactccg aagtccgcag cggcgacgtg ctcagcaccg
     3421 agcgcgacag ctacgcgcag atccgcttca gcgacggcgg ccagatcacc ctgaagccag
     3481 ggaccgccgt caagctggat gatttcaagt actccgcgga aaagccgcag gaggacgcgt
     3541 tcctgttcag cctgctgcgg ggcggcttgc gcgcggtgac cggcctggtg ggcaagcgca
     3601 accgcgaccg ctacctgatg aacaccgcca cggcgacggt cggcatccgc ggcaccacct
     3661 tctcggccga cgactgcacc ggcacgcgcg acggcgaatg cgcgcgcctg cagcaggccg
     3721 tgtacgtggg cgtgtccgac ggcgaaatcg tggtgcgcaa cgcccagggc gagctggcgc
     3781 tgggggccgg gcagttcggc catatcgcgc gcggcgagaa gccgctgttc ctgtccaccg
     3841 acccggggct gcagttcgcc ccgccggcga ccttcatcca gtcgctgctc tccggccgcc
     3901 aggtggcggg catcggcgag aagctggagt gcgcgatcag cgcgcgttga cgcctctggc
     3961 gctgcggcgc ctgcggtaaa atccgcggac ccccgaacgg gccgcgatgg cagcagcgca
     4021 gaaaaccaca gcagcgcccc ttcccgcaaa gctcgccaac ctgttgcgcg aagcccgatg
     4081 gctggtgctg gcgtcgatcg ccgtgtacct gctgctgatc ttcctcacct tccaccgcgg
     4141 cgacccgggc tggtcgctca gcgccaccgc ggccgacgtg cacaatgcgg gcggccgcgc
     4201 cggcgcctgg ctgtccaacc tgctgctgta cctgttcggc gtgtcggcct actggtggat
     4261 cgcgctgtgc ctggtgggcg tggcctgggg ctaccggtcg ctcgatcgca gcgcgctcat
     4321 cgaccgcagg ccgctggcca ttgcgctgtt cggcttcgcg ctgctgctgc tgtccagcgc
     4381 ggcgctggaa tcgctgcgcc tgcactcgct ccccgcgcag ctgccgcttg cgcccggcgg
     4441 cctgctcggc gcggagatcg gcggcttcgc cgccggcctg ctcggcttca ccggtgcgac
     4501 gctcctgctg ctgacgctag ccgccgtcgg cttcagcctg ttcaccggcg tgtcctggct
     4561 ggcggtggcg gagctcaccg gcctgctgct cgaggccgga gcgggcctgg cgcgcagcac
     4621 ctgggagcgg cgcgccgacc gcaaggcggg cgagttcgcg cgcgaggagc gcgaggtggt
     4681 ggtcgaggcg gaaaagcggc gcgaggagga tcacccgccg ctggtcatcg cgccgccggt
     4741 ggtggagatc aggaagtccg agcgcgtaca gaaggaaaag caggcgccgc tgttcgacca
     4801 tctgccggac acgccgctgc cgccggtgaa gctgctcgac gaggcggcgc aggacggcgc
     4861 ccaggtgagc gccgaatcgc tggaattcac ctcgcgcctg atcgagaaga agctctccga
     4921 tttcggcgtc tcggcgaagg tgctggcggc ctatcccggc ccggtgatca cgcgctacga
     4981 gatcgagccg gcggtcggcg tgaagggcag ccagatcatg aacctggtga aggacctggc
     5041 gcgcgccctg tcggtggtgt cgatccgcgt ggtcgagacc attcccggca agtcctgcat
     5101 ggggctggaa atccccaatc cgcaccgcca gaccgtgcgc ctgtcggaga tcctcggctc
     5161 cgagctgtac gcgggcatgc acacgccgct cgcgctggcg ctgggcaagg acatcgccgg
     5221 caacgcggtg gtggtagacc tgcagaagat gccccacctg ctggtggcgg gcaccaccgg
     5281 ctcgggcaag tcggtggcga tcaacgccat gatcctgtcg ctgctgtaca agtccgagcc
     5341 caagcaggtg cgcatgatca tggtcgaccc gaagatgctc gagctgtcca tgtaccagga
     5401 cattccgcac ctgctggcgc cggtggtgac cgacatgaag caggcggcca acgcgctcat
     5461 gtggtgcgtg gcggaaatgg agcgccgcta caagctgatg tcctgggtcg gggtgcgcaa
     5521 cctttccggc ttcaaccaca aggtggccga cgcggagaag tccggcaggc cgctcgagga
     5581 tccggcgacg ctggaatccg gcgcgccgca gccgctcggc cagctgccct acatcgtggt
     5641 ggtgatcgac gagctggccg acctgatgat ggtggtgggc aagaaggtcg aggagctgat
     5701 cgcgcggctg gcgcagaagg cgcgcgcctc gggcattcac ctgatcctcg ccacgcagcg
     5761 cccttcggtg gacgtgatca ccggcctgat caaggccaac atcccgacgc gcatcgcctt
     5821 ccaggtggcg agccgcatcg actcgcgcac catcctcgac cagcagggcg cggaagcgct
     5881 gctcggccag ggcgacatgc tgtatctgcc ggccggcacc ggcctgtcgc agcgcgtgca
     5941 cggcgccttc gtcgccgacc acgaggtgca caaggtggtc gagcacctga agtcgctcgc
     6001 cgcgcccgag tacatcgagg acatcctgga cccggccatc ggcgaggagc cgggcggcaa
     6061 cggcgccaac ggcgagccgg gcggcgagaa ggacccgctg tacgaccagg ccgtggaaat
     6121 cgtgctgcgc acgcgccggc cgtcgatttc cctcgtgcag cgccacctgc gcatcggcta
     6181 caaccgcgcc gcgcgcctga tcgaggacat ggagcgcgcc gggctggtgt cggcgatgca
     6241 gaccaacggc aaccgcgaag tgctggtgcc cgcgaaggcg gagtagggtg cgcgccgtcc
     6301 tcgcggtcgc cctgtgcgtg ccgctcttcg cctgcgccgc gggcgtggag cgcttccgcg
     6361 actacctgcg cagcacgcag accgcgcgcg ccgatttcga gcagcgggtg ttcgaccgcg
     6421 agaagaaact cgtgcagcac tcccgcggca gcttctccct gcagcgcccg gggcgcttcc
     6481 gctggaccta cgcccagccc tacccgcagc tgatcgtcgg cgacggcgcg cgcgtgtgga
     6541 tctacgacga ggacctcaac caggtgacgg tgcgtgcgat ggcgaaggcg ctggggtcga
     6601 cgcccgccgc gctgctcgcg ggggcggcgg aggtcgagcg cgcattcgcg ctcaaggacg
     6661 ccggccggcg cgacggcctg gaatgggtgg agtgcacgcc gcgggaaaag gaggcgggct
     6721 tcgagcgcat cctgctcggc atgggggcct ccggcgtgca ggcgatggaa ctcgccgacc
     6781 atttcggcca gaccacggtg ctgcgcttct ccaacatcgc gcgcaacccg gcgctcgatg
     6841 ccgccgcgtt ccgcttcacc ccgcccaagg gcgcggatgt cctcggcgac tgacctgttc
     6901 ccggcggcgg cgccggccgc gccgctcgcg gagcgcctgc ggccgcgctc gatcgacgaa
     6961 gtcctcggac agaagcacct gctcgggccc ggcaggccgc tgcgcctggc gttcgaatcc
     7021 ggcaagccgc actcgatgat cctgtggggc ccgcccggca ccggcaagac cacgctggcg
     7081 cgcctgatgg ctcaggcttt cgacgccgag ttcatcgcgc tctccgcggt gctctccggc
     7141 gtcaaggaga tccgcgacgc ggtcgcgcac gccgaggcgg tgatcgccgc cagcggacgg
     7201 cgcacgatcc tcttcgtcga cgaggtgcac cgcttcaaca aggcgcagca ggacgccttc
     7261 ctgccctacg tggagaaggg cctgttcacc ttcatcggcg ccaccaccga gaacccgtcg
     7321 ttcgaggtga actcggcgct gctctcgcgg gccaccgtgt acgtgctgga gagcctcccg
     7381 cccgaggacc tcggcgccct gatcgacaag gcaggcctcg caacggacgc cgaggcgcgc
     7441 gcgcgcctgg tcggcttcgc cgacggcgac gcgcggcgcc tgctgaacgc ggtggagatc
     7501 ctgcagaacg cgaggccgcg cggcgccatc gacgccgcct tcctcgaatc gacgctggcg
     7561 aagaacctca ggcgcttcga caagggcggc gagcagttct acgaccagat ctcggcgctg
     7621 cacaaggcgg tgcgcggctc ggacccggat gcgtcgctgt actggctggt gcgcatgctc
     7681 gacggcggcg ccgacccgct gtacctggcg cggcgcatcg tgcgcatggc ggtggaggac
     7741 gtcggcctcg ccgacccgcg cgcgctcacg ctcgccctcg aggcggcgca gacctacgag
     7801 cgcctgggct cgcccgaggg cgagctcgcg ctcgccgagg cggtgatcta catggcctgc
     7861 gcggcgaagt cgaacgcggt gtacaacgcc tataatgcgg cgcgcgcttt cgtcggggag
     7921 gacggcacgc ggccggtgcc gctgcacatc cgcaacgcgc ccaccaagct gatgaagaac
     7981 ctgggctacg gcaaggacta ccgctacgcg cacgacgagc cggacgcgtt cgccgcgggc
     8041 gagaactacc tccccgaggg catgccggcg gtggcgtggt acaagccgac cgagcgggga
     8101 ctggaggggc gcatccggga aaaactcgaa gagctcaagc ggctgaacgc cgtcgcgcgc
     8161 gggaagaaaa acaaatgatc gacatcaacc tgcttaggtc cagcttggaa ggggtcgtgt
     8221 ctcgcctgca aggtcgaggc ttgccggtgg acttcgtgcc gagattcctc g
//
LOCUS       DASRIE010000088         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3215742, whole genome shotgun sequence.
ACCESSION   DASRIE010000088 DASRIE010000000
VERSION     DASRIE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215742"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2643
                     /locus_tag="VFB53_04080"
     CDS             13..2643
                     /locus_tag="VFB53_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN85946.1"
                     /translation="MERSLFGFIIRYSKRDQLRIVPFVLLTMVVYYASLDLPKTIINQ
                     AIQGGRFEGGRETAGFLWLELGRLEYLFALSMVFLGLIVLNGWLKFRINTMKGWMGER
                     MLRRLRYVLFDHILRFPLSRFRRVKAAEMATMIKDEVEPLGGFIGEALITPLFLGGQA
                     LTAMFFIMYQHWALGLIAIAVVAVQAYLIPRLRKRLLVLGRERQLTARALAGRIAEAV
                     DGAAEIHANATSNYERAEISARLGTIFRIRFEFYQRKFLIKFLNNFLSQVTPFLFYTV
                     GGYLVIMGRLDIGALVAVIAAYKDLPSPVKELIDWDQQRLDVEVKYDQVMEQFVDEQV
                     APPEMQALIDSPRLPSGGKLRASNLTLVDEAGTRLVEDVSFEAGLEQHIAILGRPGSG
                     ASELAQLLARLVPPTSGSVELGGVDITRAPEAITGRCIGYVGSPAYLFPARVRDNILY
                     GLKHRPLKPIDDPDRARQRELELREAKRTGNSTLDIEADWIDYEAAGCSGPEDCDKRI
                     VELLDAVDLEETIFELGLRSRAQKHAGSDVAGRVLEARRHMRERLEALGISDWVERFD
                     PAAYNRNATLAENLLFGTPVGRVFDVENLALNPYVRQVLRATRLDELLIRTGHKVAET
                     MVELFSGLPPGHEFFAQYSFINQDDLPQFEAILKRAADVGLKDLNQEERRALLALPFK
                     LIASRHRLGLIDESFEARVIEARLYFADHLPPQLRGTVEFFDPERYNEAATLQDNILF
                     GKIVTGQAGATERITKLVRELLEDLGLRPMVVRNGLDYQVGVGGGRLAPAERQKIAMV
                     RAMLKRPALLVFDHAAAVLDPASQLRVVEGVLRERRGQAVAWVLSRPEYAERFDMVLV
                     MERARLVERGAFAQLKRKTG"
     gene            2640..3092
                     /locus_tag="VFB53_04085"
     CDS             2640..3092
                     /locus_tag="VFB53_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN85947.1"
                     /translation="MSLEQEVELIRQFPIFAKIQPAMQKLLCFSAERLTYAPAQVMFK
                     AGDMGDAAYIVIDGKVEISVPSPAGAIVVNTLGRNDIIGEIAIFGDVPRTATATAQAR
                     VEVLKISKELFTKIIRENPDAALELIRVLAGRLANTTAQLSRTMSGTK"
     gene            complement(3052..>4494)
                     /locus_tag="VFB53_04090"
     CDS             complement(3052..>4494)
                     /locus_tag="VFB53_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN85948.1"
                     /translation="LWEAAQLALGLAIPPLLIGHVVGTRLTWTLLGHHIDYERVVGLL
                     WSSEWSTFRQILLLLVVWAHVCAGIHYWLRVRAWYRNALPLAFAVALLLPALALSGFA
                     SAGFYLWPSIESVGGMQKYNVELAAMSAAERALMASWRSGLEYAFWVALAATLLARWL
                     RARIGATYRVRHASGRVISAPVGRSILEAIRDEGLPHAAVCGGRARCTTCRVRVGDGL
                     AHLPPPGRPEAQALGRIEAPPNVRLACQCRPTRDVAVTPLLPPNAGPSSTAPPRSGAQ
                     GRERPIVAMFVDLRDSTRLGEGRLPYDVVFIMNQFFAEMHGALRASGGYYAQFRGDGL
                     LALYGLESSLKDACRAALRGAAEMQARMDELNRALAADLEQPLRIGIGIHAGVAIVGT
                     MGPPAAPIYSAIGDMVNTAARLENMTKAFGCTLVVSDLVLKEAGLQAGNAALHQVRVR
                     GKKERLAVYAVPDPRSLVTSSRSSSATAAR"
BASE COUNT          708 a         1545 c         1609 g          632 t
ORIGIN      
        1 cggaaagcgc gcgtggaacg cagcctcttc ggattcatca tccgctacag caagcgcgac
       61 cagctgcgca tcgtgccgtt cgtgctgctg acgatggtgg tgtactacgc ctcgctcgac
      121 ctgcccaaga ccatcatcaa ccaggcgatc cagggcgggc gcttcgaggg cggccgcgaa
      181 accgccggct tcctgtggct cgagctcggc cggctggaat acctgttcgc gctctccatg
      241 gtgttcctcg ggctgatcgt gctgaacggc tggctcaagt tccgcatcaa cacgatgaag
      301 ggctggatgg gcgagcgcat gctgcgccgc ctgcgctacg tgctgttcga ccacatcctg
      361 cgcttcccgc tgtcgcgctt ccggcgcgtc aaggcggcgg agatggccac catgatcaag
      421 gacgaggtcg agccgctcgg cggcttcatc ggcgaggcgc tgatcacgcc cctcttcctc
      481 ggcggccagg cgctcaccgc gatgttcttc atcatgtacc agcactgggc gctcggcctg
      541 atcgcgatcg cggtggtggc ggtgcaggcc tatttgatcc cgcggctgcg caagcgcctc
      601 ctggtgctcg ggcgcgagcg gcaattgacg gcgcgcgcgc tcgccgggcg catcgccgag
      661 gccgtggacg gcgccgccga gatccacgcc aacgccacct cgaactacga gcgcgccgag
      721 atctcggcgc gcctcgggac gatcttccgc atccgcttcg agttctacca gcgcaagttc
      781 ctgatcaagt tcctgaacaa cttcctgtcg caggtgacgc cgttcctgtt ctacaccgtg
      841 ggcggctacc tcgtgatcat gggccggctc gatatcggcg cgctggtggc ggtgatcgcc
      901 gcgtacaagg acctgcccag cccggtgaag gagctgatcg actgggacca gcagcgcctc
      961 gacgtcgagg tgaagtacga ccaggtgatg gagcagttcg tcgacgagca ggtggcgccg
     1021 ccggagatgc aggcgctcat cgactcgccg cggctgccga gcggcggcaa gctgcgcgcc
     1081 tccaacctca cgctggtgga cgaggccggc acgcgcctgg tggaggacgt gtcgttcgag
     1141 gccgggctcg agcagcacat cgcgatcctc ggccgcccgg gctccggcgc gagcgagctc
     1201 gcgcagctgc tcgcgcgcct ggtgccgcct acgagcggct cggtggagct cggcggcgtc
     1261 gacatcacgc gcgcgcccga ggcgatcacc ggtcgctgca tcggctacgt cggctcgccg
     1321 gcgtacctgt tcccggcgcg cgtgcgcgac aacatcctct acggcctgaa gcaccgcccg
     1381 ctcaagccga tcgacgatcc ggacagggcg cggcagcgcg agctcgagct gcgcgaggcg
     1441 aagcgcaccg gcaacagcac gctcgacatc gaggccgact ggatcgacta cgaggccgcg
     1501 ggctgcagcg ggccggagga ctgcgacaag cgcatcgtgg agctgctgga cgcggtcgac
     1561 ctcgaggaga cgatcttcga gctcgggctg cgcagccggg cgcagaagca cgccggcagc
     1621 gacgtcgccg ggcgcgtgct cgaggcgcgc cggcacatgc gcgagcggct cgaggcgctc
     1681 ggcatcagcg actgggtcga gcgcttcgac cccgccgcct acaaccgcaa cgccacgctt
     1741 gccgagaacc tgctcttcgg cacgccggtc gggcgcgtgt tcgacgtcga gaacctggcg
     1801 ctcaatccct acgtgcgcca ggtgctgcgc gccacacggc tcgacgagct cctgatccgc
     1861 accggccaca aggtggccga gaccatggtc gagctcttca gcgggctgcc gccgggccac
     1921 gagttcttcg cgcagtacag cttcatcaac caggacgacc tgccgcagtt cgaggcgatc
     1981 ctcaagcgcg ccgccgacgt cggcctcaag gacctgaacc aggaagagag gcgggcgctg
     2041 ctcgcgctgc ccttcaagct gatcgcgtcc cgccaccgcc tggggctgat cgacgagagc
     2101 ttcgaggcgc gggtgatcga ggcgcgcctg tacttcgccg accacctgcc gccgcagctg
     2161 cgcggcacgg tggaattctt cgacccggag cgctataacg aagcggcgac gctgcaggac
     2221 aacatcctgt tcggcaagat cgtcaccggc caggccggcg ccaccgagcg catcaccaag
     2281 ctggtgcgcg agctgctcga ggacctcggc ctgcggccga tggtggtgcg aaacggcctg
     2341 gactaccagg tcggcgtcgg cggcgggcgc ctcgcgccgg ccgagcggca gaagatcgcg
     2401 atggtgcgcg cgatgctcaa gcgcccggcg ctgctggtgt tcgaccacgc ggcggcggtg
     2461 ctcgacccgg cctcgcagct gcgcgtggtg gagggcgtgc tgcgcgagcg ccgcggccag
     2521 gcggtggcct gggtgctgtc gcggccggag tatgcggagc gcttcgacat ggtgctggtg
     2581 atggagcgcg cgcggctggt cgagcgcggc gcgttcgcgc agctgaaaag gaaaaccgga
     2641 tgagcctgga acaggaagtg gagctgatca ggcagttccc gatcttcgcc aagatccagc
     2701 cggcgatgca gaagctcctg tgcttcagcg ccgagcgcct gacctacgcg cccgcgcagg
     2761 tgatgttcaa ggcgggcgac atgggcgacg cggcgtacat cgtgatcgac ggcaaggtcg
     2821 agatcagcgt gccgagcccc gccggcgcga tcgtggtcaa cacgctcggg cgcaacgaca
     2881 tcatcggcga gatcgcgatc ttcggcgacg tgccgcgcac cgcgaccgcg accgcgcagg
     2941 ccagggtgga ggtgctcaag atctcgaagg agctcttcac gaagatcatc cgcgagaacc
     3001 ccgacgcggc gctcgagctg atccgcgtgc ttgccgggcg gctcgccaac actaccgcgc
     3061 agctgtcgcg gacgatgagc gggacgaagt aacgagcgag cgcgggtcgg gcacggcgta
     3121 gaccgcgagg cgctctttct tgccgcgcac gcgcacctgg tgcaaagcgg cgttgcccgc
     3181 ctggagtccc gcttccttga gaacgagatc cgaaacgaca agcgtgcagc cgaacgcctt
     3241 ggtcatgttc tccaggcgcg ccgcggtgtt caccatgtcg ccgatcgcgg aatagatcgg
     3301 cgccgcgggc gggcccatcg tgccgacgat cgccacgccg gcgtggatcc cgatgccgat
     3361 gcgcagcggc tgctcgaggt cggcggcgag cgcgcggttg agctcgtcca tgcgcgcctg
     3421 catctccgcc gcgcctcgaa gcgcggcgcg gcaggcatct ttcagggaac tctcgaggcc
     3481 atagagcgcg agcaggccgt cgccgcggaa ctgcgcgtag tagccgccgc tcgcgcgcag
     3541 cgcgccgtgc atctcggcga agaactggtt catgatgaag acgacgtcgt agggcaggcg
     3601 gccttcgccc aggcgcgtgg agtcgcgcag gtcgacgaac atcgcgacga tcggccgctc
     3661 gcgcccctgc gccccgctgc gcggcggcgc cgtggaggaa gggccggcgt tcggcggcag
     3721 gagcggcgtc acggcgacgt cgcgcgtcgg ccggcactgg caggcgaggc gcacgttcgg
     3781 cggcgcctcg atgcggccga gcgcctgcgc ctccgggcgc cccggcggcg gcaggtgcgc
     3841 gagcccgtcg cccacgcgca cccggcaggt ggtgcagcgc gcgcgcccgc cgcacaccgc
     3901 ggcgtgcggc aggccctcgt cgcggatcgc ctcgaggatg ctgcgcccga ccggcgccga
     3961 gattacgcgt ccggaagcgt ggcgcacgcg gtaagtggcg ccgatgcgcg cgcgcagcca
     4021 gcgcgcgagc agcgtcgccg cgagcgcgac ccagaacgcg tactcgaggc ctgagcgcca
     4081 gctcgccatc agcgcgcgct cggccgcgct catcgcggcg agctcgacgt tgtacttctg
     4141 catgccgccg acgctctcga tgctcggcca caggtagaag ccggcgcttg cgaagccgga
     4201 cagggcaagg gcgggcagga gcagcgcgac cgcgaaggcg agcggcaggg cgttgcggta
     4261 ccacgcgcgc acgcgcagcc agtagtggat tcccgcgcag acgtgcgccc agaccacgag
     4321 gagcaggagg atctgccgga aggtggacca ctcgctcgac cagagcaggc cgaccacgcg
     4381 ctcgtagtcg atgtggtggc cgagaagcgt ccacgtgagg cgcgtgccca cgacatggcc
     4441 gatcaggagc ggcgggatgg cgagcccgag cgcgagctgc gccgcctccc acaa
//
LOCUS       DASRIE010000089         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1914254, whole genome shotgun sequence.
ACCESSION   DASRIE010000089 DASRIE010000000
VERSION     DASRIE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914254"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="ligA"
                     /locus_tag="VFB53_04095"
     CDS             complement(<1..902)
                     /gene="ligA"
                     /locus_tag="VFB53_04095"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZN85949.1"
                     /translation="MAAAKAPRARIEALRREIERHNHLYYVQDAPEISDAEYDRLFAA
                     LTQLEQEHPELASPESPTQRVGAPPLAAFPEVHHRSPMLSLANAFDEETLRAFDKRVR
                     EALGADSVEYSVEPKFDGLAVSLSYRDGLFVQGATRGDGERGEDVTANLRTVRSIPLR
                     LPRAADTAELEVRGEVLITKREFVAMNERQRAAGEKEYVNPRNAAAGSLRQLDSRISA
                     RRPLRFFAYGVGVLPAGMGRTHAGLLDRLAELGFPVAGERDVARGVEELLRYYERIGR
                     LRDGLPYAIDGVVYKVDRLDWQQRL"
     gene            complement(906..1826)
                     /locus_tag="VFB53_04100"
     CDS             complement(906..1826)
                     /locus_tag="VFB53_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HZN85950.1"
                     /translation="MSDLQIGLLVVGGAAVAAILAYNAMQERRARRGAERAFRSGHAD
                     VLLDDAAPRQEPTFEPAPRRAAAQEAALPDPRLDYVIELALKQPASVAEVAERWAPFE
                     HRFAGRAVIAASPDRLAWRPLAGAGNCAGVRAGLQLASRSGPVGEAELIEFRAAMDTL
                     AAHVGGSVSAPEVRQAVEAARVLDQLCADSDIQVVLHVVPQAGGAFGADGPAAAVDAF
                     GVARRDDGGYSLSLDVPRTAEVRRSYDAMVLHARDLCAALGGVIVDDNGRPLDERALA
                     AIGAQLEAVRESLEARGIEPGSPTALRLFA"
     gene            complement(1855..>3827)
                     /locus_tag="VFB53_04105"
     CDS             complement(1855..>3827)
                     /locus_tag="VFB53_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZN85951.1"
                     /translation="NSLSALPRFWQRVRIEPGWEVAVESVLRERLHALDLAEGSRLDA
                     VVADVPPVKAGVFRHGARAESPQLAGYTALSAKVHASSADLGGALADWFAHAYAFEGT
                     PGAAARERLPAGAMLVNRAGHQFTRHTVAFHAPDPADAGLLARQAEIEALQTKVAELA
                     QRVAAARTAETRARAALDARMAELEAARDALAERQQRRHEEQIELLKLQQAQERYRER
                     SGQIAGELAEIAAEAGRDEARRTACRAAAQRAGEEIAATRAQVEAVRTAHAGAESALA
                     AQRLAGQQAERAAQDAVFGERECSAKIAEIDGSVRLIDQQIERAEAEVAKLTEELSVD
                     PVPAVRAALETAVEVRIGREKVLAETRNAVEAAALALRGLEEERLQIEAGLAPLRERI
                     NELRLKEQAAQINHDQHAGQLSEAGADEARLAAEAERAPRPGSLQGEITRLAQAIGEL
                     GAVNLAALEELQSSQERKSYLDAQAADLDEAVRTLEDAIRRIDRETRELLRETFDSVN
                     RHFGALFPVLFGGGEAKLVMTGEEILDAGVQVMAHPPGKRNTSIHLLSGGEKALTAIA
                     LVFSMFQLNPAPFCLLDEVDAPLDDSNTLRFCDLVRKMSAQTQFLFISHNKITMEMAA
                     QLVGVTMPESGVSRVVAVDIEEALRIREELAA"
BASE COUNT          478 a         1480 c         1311 g          558 t
ORIGIN      
        1 agccgctgct gccagtcgag gcggtcgacc ttgtagacca cgccgtcgat ggcgtacggc
       61 agcccatcgc gcagcctgcc gatgcgctcg tagtagcgca gcaattcctc cacaccgcgg
      121 gcgacgtcgc gctccccggc caccggaaag ccgagttccg ccaaccggtc gagcagcccc
      181 gcatgggtgc ggcccatgcc cgcgggaagc acgcccacgc cgtaggcgaa gaagcgcaag
      241 ggccgcctgg cgctgatgcg cgaatcgagc tgccgcaggc tgccggcagc ggcgttgcgg
      301 ggattgacgt actccttttc gcccgcggcc cgctggcgtt cgttcatggc gacgaactcc
      361 cgcttggtga tcagcacctc gccgcgcact tccagttccg ccgtgtccgc ggcgcggggc
      421 aggcgcagcg ggatcgagcg cacagtgcgc aggttggcgg tgacgtcctc gccgcgctcg
      481 ccgtcgccgc gcgtggcgcc ctgcacgaac aggccgtcgc ggtacgacaa actcaccgcc
      541 aggccgtcga acttgggctc gaccgaatac tcgacgctgt ccgcgcccag cgcctcgcgc
      601 acgcgcttgt cgaaggcgcg cagtgtttcc tcgtcgaagg cgttggcgag ggacagcatc
      661 gggctgcggt ggtgcacttc cgggaatgcc gccagcggcg gcgcgcccac gcgctgcgtc
      721 ggcgattcag ggctggccag ctccgggtgc tcctgctcca gctgcgtcaa tgcggcgaac
      781 aggcggtcgt attcggcgtc cgagatctcg ggcgcgtcct gcacgtaata caggtggttg
      841 tgccgttcga tttcgcggcg caacgcctcg atgcgcgcgc gcggggcctt ggccgccgcc
      901 atccttcagg cgaacagccg cagcgccgtc gggctgccgg gctcgatgcc ccgcgcttcc
      961 agcgattcgc gcaccgcttc gagctgcgcg ccgatcgccg ccagcgcgcg ctcgtcgagc
     1021 ggccggccgt tgtcgtccac gatcacgccg cccagcgccg cgcacaggtc gcgcgcatgc
     1081 agcaccatgg cgtcgtagct gcggcgcacc tccgcggtgc gcggcacgtc gagggacagc
     1141 gagtagccgc cgtcgtcgcg gcgcgccacg ccgaaggcat ccaccgccgc cgccggaccg
     1201 tcggcgccga acgccccgcc cgcctgcggc accacgtgca gcaccacctg gatgtccgag
     1261 tccgcgcaca gctgatccag cacgcgcgcg gcctcgacgg cctgcctgac ttcgggcgcc
     1321 gaaacgctgc cccccacgtg cgcggcgagg gtatccatcg cggcgcggaa ctcgatcaat
     1381 tccgcttcgc ccaccggccc gctgcggctg gccagctgca gcccggcgcg caccccggcg
     1441 caattgccgg cgccggcgag cggacgccag gccagccggt ccggactggc cgcgatgacc
     1501 gcgcgcccgg cgaagcggtg ctcgaacggc gcccagcgct cggcgacttc ggccacggag
     1561 gcgggctgct taagagccag ttcgatgacg tagtccaggc gcgggtcggg cagcgccgcc
     1621 tcctgcgcgg cggcgcgccg cggcgcaggt tcgaaggtcg gttcctggcg cggcgccgcg
     1681 tcgtcgagca gcacatcggc gtgcccggat cggaaggcgc gctcggcgcc gcggcgcgcg
     1741 cggcgttcct gcatggcgtt gtaggcaagg atcgccgcca ccgccgcccc tccgaccacc
     1801 agcaggccga tctgcaaatc gctcatggcc aggccggcga ccggctcgcc gcgctcaggc
     1861 ggcgagctcc tcccggatgc gcagcgcttc ctcgatgtcc accgccacca cgcgcgacac
     1921 gcccgactcg ggcatggtga cgccgaccag ctgcgcggcc atttccatgg tgatcttgtt
     1981 gtggctgatg aacaggaact gcgtctgcgc cgacatcttg cgcaccaggt cgcagaagcg
     2041 caaggtattg ctgtcgtcca ggggcgcgtc gacctcgtcc agcaggcaga aaggcgccgg
     2101 attgagctgg aacatggaaa acaccagcgc gatcgccgtc agcgcctttt cgccgcccga
     2161 cagcaggtgg atgctggtat tgcgcttgcc cggcgggtgc gccatgacct gcacgcccgc
     2221 gtcgaggatc tcctcgccgg tcatcaccag cttcgcctcg ccgccgccga acagcaccgg
     2281 gaacagcgcg ccgaaatgcc gattcacgct gtcgaaggtc tcgcgcagca actcgcgcgt
     2341 ctcgcggtcg atgcgccgga tggcgtcctc cagcgtgcgc accgcctcgt ccaggtccgc
     2401 ggcctgcgca tcgaggtagc tcttgcgctc ctggctggac tgcagctcct ccagcgccgc
     2461 caggttgacc gcgcccagtt cgccgatcgc ctgcgcgagc cgggtgattt cgccctgcag
     2521 agaaccgggc cgcggcgcgc gctcggcttc ggcggccagc cgcgcctcgt ccgcccccgc
     2581 ctcgctcagc tgcccggcgt gctggtcgtg gttgatctgc gccgcctgct ccttgaggcg
     2641 cagttcattg atgcgctcgc gcagcggcgc caggccggcc tcgatctgca gccgctcctc
     2701 ctccagcccg cgcagcgcca gcgccgccgc ctcgaccgcg ttgcgcgtct cggccagcac
     2761 cttctcccgg ccgatgcgca cctcgaccgc cgtctccagc gccgcgcgca ccgcgggcac
     2821 cgggtccacc gacagttcct cggtgagctt cgccacctcg gcttcggcgc gctcgatctg
     2881 ctggtcgatg agccgcaccg agccgtcgat ctcggcgatc ttggcgctgc actcgcgctc
     2941 gccgaacacc gcgtcctgcg ctgcgcgctc ggcctgctgg ccggccaggc gctgcgcggc
     3001 cagcgcactc tcggcgccgg catgggcggt gcgcaccgcc tcgacctggg cccgcgtcgc
     3061 ggcaatttcc tcgccggcgc gctgcgcggc agcccggcac gcggtgcgcc gcgcctcgtc
     3121 gcgcccggct tcggcggcga tctcggcgag ctcgcccgcg atctgtccgc tgcgctcgcg
     3181 gtagcgttcc tgcgcctgct gcagcttgag cagctcgatc tgctcctcat ggcgccgctg
     3241 ctggcgttcg gccagcgcat cgcgcgccgc ttccagctcg gccatgcgcg catccagcgc
     3301 cgcgcgggcc cgcgtttcgg cggtccgcgc ggcggccacg cgctgcgcca gctccgcgac
     3361 tttcgtctgc agcgcctcga tctccgcttg ccgcgcgagc agcccggcgt ccgccgggtc
     3421 cggcgcgtgg aaggcgacgg tatggcgcgt gaactggtgg ccggcgcgat tcaccagcat
     3481 ggcgccggcg ggcaggcgct cccgcgccgc ggcgcccggc gtgccttcga aggcataggc
     3541 gtgcgcaaac cagtcggcca gcgcgccccc caggtccgcg ctgctcgcgt gcaccttggc
     3601 gctcagcgcc gtgtagccgg cgagctgggg cgactcggcc cgggcgccat ggcggaacac
     3661 gcccgccttc accgggggca cgtccgccac cacggcatcc aggcgcgagc cttcggcgag
     3721 gtcgagcgcg tgcagccttt cgcgcagcac cgattcgacc gccacttccc agcccggctc
     3781 gatgcgcacg cgctgccaga agcgcggcaa cgcgctcagg ctgttgc
//
LOCUS       DASRIE010000090         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_871333, whole genome shotgun sequence.
ACCESSION   DASRIE010000090 DASRIE010000000
VERSION     DASRIE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871333"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1088
                     /locus_tag="VFB53_04110"
     CDS             138..1088
                     /locus_tag="VFB53_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN85952.1"
                     /translation="MKDLSKPQATWLEVGEESEAQRIDNFLLRRLKGVPKSHVYRVLR
                     SGEVRVNSGRVKPDYRLQAGDRVRVPPVRLATPAPRPAPRPLALPVVYEDAALLVIDK
                     PSGVAVHGGSGVSYGVIESLRAERPAAKSLELAHRLDRDTSGLLIIAKKRGALVELHR
                     MLRDGEVEKAYAALVQGRWQGGGRELRERLHKFVNAGGERRVAVHAAGKTAVTQVKPL
                     AAGDDFSLLELRLLTGRTHQIRVHLAHAGHPVLGDDKYGDFALNHALAKQGVKRLLLH
                     ARRLAFAHPLTREAMRLEAPLPADMRRFAEERLALPPDRL"
     gene            1063..1713
                     /locus_tag="VFB53_04115"
     CDS             1063..1713
                     /locus_tag="VFB53_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN85953.1"
                     /translation="MRYPLIVFDWDGTLLDSTGGIADCIQAAARDLHLPVPARATASH
                     VIGLGLQASLRTAVPSLPEDQYREFAEAYRRHFLARQGSMALFPGVREMLQELHGAGH
                     RLAVATGKSRRGLDHALESTALRRMFAASRCADETDPKPHPAMLLELMEQLRVAPAQA
                     LMIGDTSHDLEMARSAGVDAVAVAYGAHPGEALRALAPRACVADVAQLRTWLTANA"
     gene            1692..2048
                     /locus_tag="VFB53_04120"
     CDS             1692..2048
                     /locus_tag="VFB53_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN85954.1"
                     /translation="MADGERLICASAALQEAGKGVRFEVEYFGDAAPAFVVRAGGRVR
                     GYLNRCAHVAMELDWREGEFFDLQGRDLICSTHGAVYAAESGRCLGGPCSGRPLVRLR
                     IEERAGQVYYTGVDDD"
     gene            2041..2985
                     /locus_tag="VFB53_04125"
     CDS             2041..2985
                     /locus_tag="VFB53_04125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZN85955.1"
                     /translation="MTDNDGQWERGLIEKLAGAALKEQRSARRWGIFFKLLTFAYVTV
                     IIVLAVDWKGGSGDAAGRHTALVEVSGIIAPGADASAERVTNALQAAFKDKNTVGVLV
                     RINSPGGSPVQAQNIYEEMRRLRKKHPGIPLHAVVEDLCASGGYYVAAGADRIFVSRS
                     SIVGSIGVRMDSFGVTGLMDKLGVERRLLTAGDNKGFLDPFLPVDEKQKQHAQALLSD
                     VHQQFIGVVREGRGKRLKETPDMFSGLIWTGQKSVELGLADAFGSLEYVAREVIKAEH
                     IVDYTQKENIAEKFARRFGAGAAGALLELALRQAPQLR"
     gene            complement(2982..3668)
                     /locus_tag="VFB53_04130"
     CDS             complement(2982..3668)
                     /locus_tag="VFB53_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN85956.1"
                     /translation="MRPALYLVPAPLGGPALDSLPAASAAIVRTLRDFAVENAKSARA
                     TLAALGMPCTMRELSIVEIGSEAAPLLAPLRAGRSLGVLSEAGSPAVADPGAALVAAA
                     HAEGFRVVPLVGPSALLLALMASGLEGQRFAFCGYLPRDQKALAARIRALEKRSGAER
                     ETQIFIETPYRGDALLAALLEHARPGTALCVAADLTLPTESIATRTVAQWRAAGAHIG
                     RRPAVFLLLA"
     gene            complement(3665..4258)
                     /locus_tag="VFB53_04135"
     CDS             complement(3665..4258)
                     /locus_tag="VFB53_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HZN85957.1"
                     /translation="MPEPRPLILASTSRYRKALLERLGLPFDCADPGIDERALPAESA
                     AATAARLAALKARAVAGRHPQALIIGSDQVASCDGAILDKPGTHANAVRQLAALSGRS
                     ARFDTALALFDARSGEERSSVVPCLVHFRRLDAAQIEDYLRREQPYDCAGSAKSEGLG
                     IALIARIEGEDPTALIGLPLIALTAMLAQAGMPVLRG"
     gene            4308..4820
                     /locus_tag="VFB53_04140"
     CDS             4308..4820
                     /locus_tag="VFB53_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZN85958.1"
                     /translation="MPQQPARFPLIDGFEFAAAGGSVRGAWPVSVFPRLREVLQDDSG
                     AVEYAVRGGRDIHGRAQLALHAKARLQLTCQRCLGAVECVLEQRSTLLLAATQAEIDA
                     EPITPQMPERVVARRDMAVRDLVEDELLLALPYAPRHDNCAAQDKAAPKGRQMPFATL
                     RGMLRGKSGH"
     gene            4834..5013
                     /gene="rpmF"
                     /locus_tag="VFB53_04145"
     CDS             4834..5013
                     /gene="rpmF"
                     /locus_tag="VFB53_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZN85959.1"
                     /translation="MAVQQNKKSPSRRGMHRAHDFLGNPPLAVEPTTGEVHLRHHVSP
                     TGYYRGKKVAKGKGE"
     gene            5064..>5353
                     /locus_tag="VFB53_04150"
     CDS             5064..>5353
                     /locus_tag="VFB53_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZN85960.1"
                     /translation="MDISVAIDCMGGDHGPRVTVPAALALQRAHADVAVVLVGPRAAL
                     EAELAAHGASPGPRLHVHHASEVVRMDEPPAQALRYKKDSSMRVAVNLVKTG"
BASE COUNT          793 a         1811 c         2037 g          712 t
ORIGIN      
        1 cctggttcgg gtccggctcg cggccggaca ggaggcgggt tgcagggacc ggcgcgtggg
       61 tagcgttgct acggcctgac gcgtcttgtg gtccctgtgc ctcgcaactc gaggccagac
      121 cacggataat aataaaaatg aaggacttaa gcaagccgca ggcgacctgg cttgaagtcg
      181 gcgaagaaag cgaagcgcag cgcatcgaca acttcctgct gcgccggctc aagggggtgc
      241 ccaagagcca tgtctaccgg gtgctgcgca gcggcgaggt ccgggtcaac agcggccggg
      301 tgaagccgga ctaccgcctg caggccggcg accgggtgcg ggtgcccccg gtgcgcctgg
      361 cgaccccggc gcccaggccg gcgccgcggc cgctcgccct gccggtggtg tacgaggacg
      421 ccgcgctgct ggtgatcgac aagccctccg gcgtcgccgt ccacggcggc agcggcgtga
      481 gctacggggt gatcgaatcg ctgcgcgcgg agcggcccgc ggccaagtcc ctggaactcg
      541 cccaccgcct ggaccgggac acctcgggcc tgctgatcat cgccaagaag cgcggcgcgc
      601 tggtcgagct gcaccgcatg ctgcgcgatg gcgaagtgga aaaggcctac gccgctctgg
      661 tgcagggccg ctggcagggc ggcggccgcg agctgcgcga gcggctgcac aagttcgtca
      721 atgccggggg cgagcggcgc gtcgcggtgc acgcggcggg gaagacggcg gtgacgcagg
      781 tcaagccgct ggcggcgggc gacgatttca gcctgctcga gctgcgcctg ctgaccgggc
      841 gcacccacca gatccgggtg catctggcgc acgccgggca tccggtcctc ggcgacgaca
      901 agtacggcga cttcgcgctc aaccatgcgc tggcgaagca gggtgtgaag cggctgctgc
      961 tgcatgcccg ccgcctggcg ttcgcccatc ccctgacgcg ggaggcgatg cgcctcgagg
     1021 cgccactgcc ggccgacatg cgccgcttcg cggaggagcg ccttgcgcta ccccctgatc
     1081 gtctttgact gggacggcac gctcctcgac tcgacgggcg gcatcgccga ttgcatccag
     1141 gcggcggccc gcgacctgca tctgccggtg ccggcgcgcg caacggccag ccacgtcatc
     1201 ggactggggc tgcaggcctc gctgcgcacc gcggtgccga gcctgccgga ggaccagtac
     1261 cgcgaattcg ccgaggccta ccgccggcat ttcctggcgc ggcagggctc gatggcgctg
     1321 tttcccgggg tgcgcgagat gctgcaggaa ctgcacggcg ccggccaccg cctcgcggtg
     1381 gccacgggca agagccgccg cggcctggac catgccctcg aatcgaccgc cctgcgcagg
     1441 atgttcgccg cctcgcgctg cgcggacgaa accgatccga agccgcatcc ggcgatgctg
     1501 ctggagctga tggagcagct gcgggtggcg cccgcgcagg cgctgatgat cggcgacact
     1561 tcgcacgacc tggagatggc gcgcagcgcg ggcgtggatg cggtggccgt cgcctacggc
     1621 gcgcacccgg gcgaggcgct gcgcgccctg gcgccgcgcg cctgcgtggc ggacgtcgcg
     1681 caactgcgga catggctgac ggcgaacgcc tgatctgcgc ctcggccgcg ctgcaggagg
     1741 cgggcaaggg ggtgcgcttc gaagtcgagt acttcggcga cgccgcgccc gcgttcgtgg
     1801 tgcgcgccgg gggacgcgtg cgcggctatc tcaaccgctg cgcgcacgtg gcgatggagc
     1861 tggattggcg cgagggcgag ttcttcgatc tgcaggggcg cgatttgata tgctcgacgc
     1921 acggtgccgt ctacgccgcc gagagcggcc gttgcctggg cggcccctgc agcggcaggc
     1981 cgctggtgcg gctcaggatc gaggagcgcg ccgggcaggt ctactacacg ggagtcgacg
     2041 atgactgaca acgacgggca gtgggagcgc gggctgatcg agaaactggc cggcgcggcg
     2101 ctcaaggagc agcgcagcgc gcgccggtgg ggcatcttct tcaagctgct gaccttcgcc
     2161 tatgtcacgg tgatcatcgt gctggcggtg gactggaagg gcggcagcgg cgacgcggcc
     2221 ggccggcata ccgcgctggt ggaggtgagc ggcatcatcg cgcccggcgc cgacgccagc
     2281 gccgagcgcg tgaccaacgc gctgcaggcc gccttcaagg acaagaacac cgtgggcgtg
     2341 ctggtgcgca tcaacagccc cggcggcagc ccggtgcagg cgcagaacat ctacgaggag
     2401 atgcgccgcc tgcgcaagaa gcatcccggc atcccgctgc acgcggtggt ggaggacctg
     2461 tgcgcctcgg gcggctacta cgtggccgcc ggcgcggacc gcatcttcgt ctcgcgctcg
     2521 agcatcgtcg gctcgatcgg cgtgcgcatg gacagcttcg gcgtcaccgg cctgatggac
     2581 aagctgggcg tcgagcggcg tctgctcacc gctggcgaca acaaggggtt cctcgacccc
     2641 ttcctgccgg tggacgagaa gcagaagcaa catgcgcagg cgctgctcag cgacgtgcac
     2701 cagcagttca tcggcgtggt gcgcgagggc cgcggcaagc gcctgaagga gaccccggac
     2761 atgttctcgg ggctgatctg gaccgggcag aagagcgtgg agctggggct ggcggacgcc
     2821 ttcggcagcc tggagtacgt ggcgcgcgag gtgatcaagg ccgagcacat cgtcgactac
     2881 acgcagaagg agaacatcgc cgagaagttc gcgcggcgct tcggcgccgg cgccgccggc
     2941 gcgctgctcg agctggcgct gcggcaggcg ccgcagctgc gctaggcgag cagcaggaac
     3001 accgcgggcc ggcgcccgat gtgcgcgcct gcggcgcgcc actgcgccac cgtgcgcgtg
     3061 gcgatcgact cggtgggcag ggtcaggtcg gccgccacgc agagcgcggt cccgggccgc
     3121 gcgtgctcga gcagggccgc gagcagcgca tcgccgcggt agggcgtctc gatgaaaatc
     3181 tgcgtctcgc gttcggcgcc cgagcgtttc tcgagcgcgc ggatgcgcgc ggcgagcgcc
     3241 ttctggtcgc gcggcaggta gccgcagaac gcgaagcgct gcccctccag ccccgaggcc
     3301 atcagcgcca ggaggagggc ggagggaccc accagcggca ccacgcggaa accctccgca
     3361 tgcgccgcgg cgaccagcgc ggcgcccggg tcggccaccg ccgggctgcc ggcctccgac
     3421 agcacgccca gcgagcgccc ggcgcgcagg ggggcgagca gcggggcggc ctcgctgccg
     3481 atctccacga tgctcagctc gcgcatcgtg cagggcattc ccagggcggc gagggtggcg
     3541 cgtgcgctct tggcattctc cacggcaaaa tcgcgcaggg tgcgcacgat cgccgcagac
     3601 gctgcgggga gggaatcgag cgccggtccg ccgagcggcg cgggcaccag gtacagggcc
     3661 ggcctcaccc gcgcagcacc ggcatgcccg cctgcgccag catggcggtc agcgcgatca
     3721 gcggcaagcc gatcagcgcg gtcggatcct cgccttcgat gcgtgcgatg agcgcgatcc
     3781 ccaggccttc ggatttggcg ctgccggcgc aatcgtaggg ttgctcgcgc cgcaggtagt
     3841 cctcgatctg cgccgcgtcc aggcggcgga aatgcaccag gcagggaacc accgacgagc
     3901 gttcctcgcc gctgcgcgcg tcgaacagcg ccagcgcggt gtcaaagcgc gcgctgcggc
     3961 cgctcagcgc ggcgagctgg cgcaccgcgt tggcatgcgt gccgggcttg tcgaggatcg
     4021 cgccgtcgca ggaggccacc tggtccgaac cgatgatcag cgcctgcgga tgacggcccg
     4081 ccactgcgcg cgctttcagc gcggcgaggc gcgccgcggt ggcggcggcg gattcggcgg
     4141 gcagcgcgcg ctcgtcgatc ccggggtcgg cgcagtcgaa gggcagcccg aggcgttcca
     4201 gcagcgcctt gcggtagcgg gaggtcgaag cgagtatcag cggccggggc tcaggcatga
     4261 ctttgttttg acacggtttt tgcggcgatg atatcattcg cgccctcatg ccgcagcagc
     4321 cggcgcggtt tcccctgatc gacggcttcg agtttgcggc agccggcggc agcgtacgcg
     4381 gcgcctggcc ggtgagcgtt tttccccggc tgcgcgaggt gttgcaggac gactcgggtg
     4441 cggtcgagta cgcagtgcgg ggcggccgcg acatccatgg gcgggcgcag ctggcgctgc
     4501 acgcgaaggc gcggctgcag ctgacttgcc agcgctgcct gggggcggtg gaatgcgtgc
     4561 tggagcagcg ctcgacgctg ttgctggcgg cgacgcaggc ggagatcgac gcggagccga
     4621 tcacgccgca gatgcccgag cgcgtggtgg cgcggcgcga catggcggtg cgcgacctgg
     4681 tggaagacga attgctgctg gcgctgccgt atgcgccgcg gcacgacaac tgcgcggcgc
     4741 aggacaaggc agcgccgaag ggacggcaga tgccgttcgc aacgctgcgc ggcatgctgc
     4801 gcggcaagag tggacactga gaggagcaag cacatggcag tccagcagaa caagaaatcc
     4861 ccgtcgcgcc gcggcatgca tcgcgcgcac gacttcctcg gcaacccgcc cctggcggtg
     4921 gagccgacga ccggcgaggt gcatctgcgc caccacgtca gtccgaccgg ctactaccgg
     4981 ggcaagaagg tcgcgaaggg caagggcgag taagcgcgcg gtcgttcgag ccccgcgccc
     5041 cgcgcgcggg cgcccgaggc tcgatggaca tctccgtcgc catcgactgc atggggggcg
     5101 accacggccc ccgcgtgacg gtccccgccg cgcttgcgct gcagcgcgcg catgccgacg
     5161 tcgcggtggt gctggtcggc ccgcgcgccg cgctcgaggc ggagctcgcg gcgcacggcg
     5221 cgtccccggg gccgcggctg catgtgcatc acgcgagcga ggtggtgcgc atggacgagc
     5281 cgccggcgca ggcgctgcgc tacaagaagg actcctcgat gcgcgtcgcc gtcaacctgg
     5341 tgaagaccgg aga
//
LOCUS       DASRIE010000091         5879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1408976, whole genome shotgun sequence.
ACCESSION   DASRIE010000091 DASRIE010000000
VERSION     DASRIE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408976"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFB53_04155"
     CDS             complement(<1..512)
                     /locus_tag="VFB53_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZN85961.1"
                     /translation="MRRLRARLPLGPVILDPLGAELTEDDRRRLLHPAAGGVILFSRS
                     YQDSGQLQSLTEEIRALREPQLLVGVDHEGGRVQRFQAGFTALPPMRSLGRLWDRDRA
                     AARAAARAAGYVIAAELAAHGLDFSFTPVLDLDYGTSAVIGDRALHFDPLAAGALAAA
                     LIGGLDEGGMP"
     gene            complement(500..883)
                     /gene="acpS"
                     /locus_tag="VFB53_04160"
     CDS             complement(500..883)
                     /gene="acpS"
                     /locus_tag="VFB53_04160"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZN85962.1"
                     /translation="MIYGVGTDLIEIPRVERALARFGERFARRILCGPELQRFHGHGK
                     PAAYLAKRFAAKEAFTKALGTGIHAPANWHGVWVVNLESGKPQLEFSAALALLLRERG
                     IRRSHLTLSDERGLAAATVILECDG"
     gene            complement(880..1626)
                     /locus_tag="VFB53_04165"
     CDS             complement(880..1626)
                     /locus_tag="VFB53_04165"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZN85963.1"
                     /translation="MIELGVNVDHVATVRQARRSHEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRELTHIKLNLEMAATEEMIVIAGRIKPEMAMLVPEGRLEVTTE
                     GGLDILAQEAHLRQAVGRLAAADIVVSVFIDADLRQVEAAKRIGAAVCEVHTGPYAAA
                     FHSRGRDAESPAVAGELQRIRAAGEAIRGLGMRFNAGHALNYFNVDPVARLPGLRELH
                     IGHAIVSRAVFVGMREAVREMKQLIVQAAR"
     gene            complement(1623..2354)
                     /gene="recO"
                     /locus_tag="VFB53_04170"
     CDS             complement(1623..2354)
                     /gene="recO"
                     /locus_tag="VFB53_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZN85964.1"
                     /translation="MTRQRVQLEPAFVLHSHPYKETSLIVEAFTRHRGRVGLLARGAR
                     RPRSALRGILLAFHPLRLSWSASAELGTLQAAEWTGGQRALAGLGLMCGFYLNELLLR
                     LLPRDDAHEKLFDAYAETVARLAGSGEHAVLLRGFELRLLTELGYAPPLDREAGSGKP
                     VQPGTRYAYAPERGPLAVNGGARGAEGEDLVVDGQTLLDMARDDYARPETREQARRLM
                     RRLIAVRLAGQELHTRQMLRELQDL"
     gene            complement(2351..3235)
                     /gene="era"
                     /locus_tag="VFB53_04175"
     CDS             complement(2351..3235)
                     /gene="era"
                     /locus_tag="VFB53_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZN85965.1"
                     /translation="MSAFRSGSVGFVGRPNTGKSSLLNRLVGQKLAIVSPREQTTRHL
                     LTGILNTADCQIAFIDSPGFQTRHRNLLNRSLNRRAGEVARDVDVAAFVLEALRLDGA
                     DRAALARVPAGHPVVAVVNKVDLVRPAARLLPFLQRLAQEREFATIVPVSAATSRNLP
                     ELLRVLREQLPEGPAHYPAEQLTDRDERFFAAELLREKIFRLLGEELPYRCDVIVDSF
                     REEGRLRRIEATLWVERDSHKPIVLGKGGAMLKRLSTDARKDMEGLYGGKVFLQVWVK
                     VKRNWTEDARTLRELGYA"
     gene            complement(3232..3903)
                     /gene="rnc"
                     /locus_tag="VFB53_04180"
     CDS             complement(3232..3903)
                     /gene="rnc"
                     /locus_tag="VFB53_04180"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZN85966.1"
                     /translation="MSRTATLEDRLGHRFARRELLEQALTHRSHGSPHNERLEFLGDG
                     VLGCVVAEALLARHPAMSEGELSRLRASLVREESLAALAGELGLAAQLRLGQGESASG
                     GAQRPSILADALEALYGAVFLDAGYDGARRAVLGTLGAALEGAGEGGAAKDAKTRLQE
                     ALQARRHGLPRYAVVATLGAAHQQTFEVECVAEELGLRARGKGGSRRAAEQEAAAALL
                     GLLER"
     gene            complement(3923..4282)
                     /locus_tag="VFB53_04185"
     CDS             complement(3923..4282)
                     /locus_tag="VFB53_04185"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZN85967.1"
                     /translation="MRNRQRGITLMGLLIGSVVVLFAALLAMKLLPAYIEYFAIKKAV
                     NGITLESRGRGSAVSDLRRAFDARAAIDDFTAVKASDLEINKEGDGFTVTAAYRKEVH
                     LFHNIGVYIDFTATSSN"
     gene            complement(4309..5097)
                     /gene="lepB"
                     /locus_tag="VFB53_04190"
     CDS             complement(4309..5097)
                     /gene="lepB"
                     /locus_tag="VFB53_04190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZN85968.1"
                     /translation="MNFALFLLVLLLVTGGVWAAEAAWLARRRPAEARQPWWVEYSVS
                     FFPVILIVFLLRSFLVEPFKIPSGSMIPTLLVGDFILVNKFAYGIRLPVANRKLIQIG
                     DPQRGDVMVFRYPDDSSLDYIKRVVGLPGDRVEYRNKRLTINGQAVPVKQIEDYLSRE
                     RMQYSRRYLETLNGTEHQVMIEDDAPVFVPQARAFPYSGNCNYNMSGLACTVPPGHYF
                     VMGDNRDNSSDSRVWGFVPDENIVGKAFFIWLNFSELGRFGAFR"
     gene            complement(5100..>5879)
                     /locus_tag="VFB53_04195"
     CDS             complement(5100..>5879)
                     /locus_tag="VFB53_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HZN85969.1"
                     /translation="RCGFLGLLHMDIVQERLEREYGMELVTTAPSVVYEVHLRDGGVQ
                     LVSNPAKLPDPSLIAEIREPMIATTLFVPQEYVGPVMTLCTEKRGTQTNLHYSARHVV
                     LSYDLPLNEVVLDFFDRLKSLTRGFGSMDYEFKAYRPADVIRLDVLVNGERVDALSSM
                     VHREVAQRRGRELAARLRGLIPRQMFDVAIQAAIGANIIARENVKALRKNVLAKCYGG
                     DVTRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAVLQRDAD"
BASE COUNT          852 a         2128 c         2019 g          880 t
ORIGIN      
        1 ggcatgccgc cctcgtccag cccgccgatc agcgcggccg ccagcgcgcc cgcggccaga
       61 ggatcgaagt gcagcgcccg gtcgccgatc accgcgctcg tgccgtagtc gaggtcgagc
      121 accggggtga agctgaaatc cagcccgtgc gccgccagct ccgcggcgat gacgtagccg
      181 gcggcgcgcg ccgccgcgcg cgccgccgcc cgatcccggt cccacagcct gccaagcgag
      241 cgcatcggcg gcagcgcggt gaagccggcc tggaagcgct gcacccgccc gccctcgtgg
      301 tccacgccga ccagcagctg cggctcgcgc agcgcacgga tctcctccgt cagggactgc
      361 agttgcccgg agtcctggta gctgcggctg aacaggatca cgccgcccgc cgccggatgc
      421 agcaggcggc gccggtcatc ctcggtcagt tccgctccca gcgggtccag gatcaccggg
      481 cccaggggca gccgcgccct cagccgtcgc attcgaggat cacggtcgcg gcggccaggc
      541 cgcgctcgtc gctgagcgtg aggtgggaac ggcggatgcc gcgttcgcgc agcaggagcg
      601 cgagcgcggc ggagaactcc agctgcggct tgcccgactc gaggttgacg acccagacgc
      661 catgccagtt cgccggcgcg tggatgcccg ttcccagcgc cttggtgaag gcctccttgg
      721 ccgcaaagcg ctttgccagg taggcggcgg gcttgccgtg cccgtgaaag cgctgcagct
      781 ccgggccgca caggatgcgg cgcgcgaacc gctcgccgaa gcgcgccagc gcccgctcga
      841 cgcgcgggat ttcgatcagg tcggtgccga cgccgtagat caccttgcag cctggacgat
      901 cagctgcttc atctcgcgca ccgcttcgcg catgccgacg aacacggcgc ggctgacgat
      961 ggcgtggccg atgtgcagtt cgcgcaaccc cggcaggcgc gccaccgggt cgacgttgaa
     1021 gtagttcagc gcatgcccgg cgttgaagcg catgccgagg ccgcggatcg cctcccccgc
     1081 cgcgcggatg cgctgcaatt cgcccgccac cgccgggctc tcggcgtcgc ggcccctgga
     1141 atggaaggcc gcggcgtagg gcccggtatg cacctcgcag accgcggcgc cgatgcgctt
     1201 tgccgcctcg acctggcgca ggtcggcgtc gatgaacacg gacaccacga tatcggctgc
     1261 cgccaggcgc ccgacggcct gccgcaggtg ggcctcctgg gcgaggatgt cgaggccgcc
     1321 ctcggtggtc acctcgaggc gcccttcggg gaccagcatc gccatttccg gcttgatgcg
     1381 gccggcgatc acgatcatct cctcggtcgc ggccatctcc aggttgagct tgatgtgcgt
     1441 cagctcgcgc aggcggcgca cgtcctcgtc ctggatgtgg cgccggtcct cgcgcaggtg
     1501 cacggtgatg ccgtccgcgc cccccaggtg cgcctccacg gcggcccaca ccgggtccgg
     1561 ctcgtggctg cgccgcgcct ggcgcaccgt ggcgacgtgg tcgacgttga cgcccagctc
     1621 gatcataggt cctgcagctc ccgcagcatc tgcctggtgt gcaactcctg gcccgccagg
     1681 cgcaccgcga tcagccggcg catcaggcgg cgggcctgct cgcgcgtttc cggccgcgca
     1741 tagtcgtcgc gcgccatgtc gagcagcgtc tgcccgtcga cgaccaggtc ctcgccctcg
     1801 gcgccgcgcg cgccgccgtt gaccgccagc gggccgcgct cgggcgcgta ggcgtagcgc
     1861 gtcccgggct gcaccggttt gccgctcccg gcctcgcgat ccagcggcgg agcatacccg
     1921 agttcggtca gcagcctgag ctcgaagccg cgcagcagca ccgcgtgctc gccgctgccg
     1981 gcgagccgcg cgaccgtctc cgcataggcg tcgaacaact tctcgtgcgc atcgtcgcgc
     2041 ggcagcaggc gcagcagaag ttcgttcagg tagaacccgc acatcaggcc caggccggcc
     2101 agcgcgcgct gcccgcccgt ccattccgcg gcctgcaggg tgcccagctc ggccgatgcc
     2161 gaccagctca ggcgcaaggg atggaacgcg agcaggatgc cgcgcagtgc cgaacgcggg
     2221 cggcgggcgc cccgcgccag cagcccgacg cggccgcgat ggcgcgtgaa cgcctccacg
     2281 atcaggctgg tctccttgta cgggtggctg tgcaggacga aggccggctc cagctgcacg
     2341 cgttgccgcg tcatgcgtag cccagctcgc gcagcgtgcg cgcatcctct gtccagttgc
     2401 gcttcacctt gacccagacc tgcaggaaca ccttgccccc gtacagccct tccatgtcct
     2461 tgcgcgcgtc ggtcgacagg cgcttgagca tcgcgccgcc cttgcccagc acgatcggct
     2521 tgtgactgtc gcgctccacc cagagggtcg cctcgatgcg ccgcaggcgc ccctcctcgc
     2581 ggaagctgtc gacgatcacg tcgcagcggt acggcagttc ctcgccgagc aggcggaaga
     2641 tcttctcgcg cagcagctcc gcggcgaaga agcgttcgtc gcggtcggtc agctgctccg
     2701 cggggtaatg cgctggtccc tccggcaact gctcgcgcag cacgcgcagc agctccggca
     2761 ggttgcggct cgtggccgcc gacaccggga cgatggtggc gaactcgcgc tcctgcgcca
     2821 ggcgctgcag gaacggcagc agccgggcgg cgggccgcac cagatcgacc ttgttgacga
     2881 ccgcgaccac cgggtggccc gcgggaaccc gcgccagggc ggcgcggtcc gcgccgtcca
     2941 gccgcagggc ttccagcacg aatgccgcca cgtcgacgtc gcgcgccacc tcccctgccc
     3001 ggcggttgag cgagcggttg agcaggttgc ggtggcgcgt ctggaagccg ggcgaatcga
     3061 tgaatgcgat ctggcaatcg gcggtattga ggatgccggt gagcaggtgg cgcgtggtct
     3121 gctcgcgcgg cgacacgatc gccagcttct gccccaccag gcggttgagc aggctggact
     3181 ttcccgtgtt cggccggccg acgaagccga cgctgccgct gcggaacgcg ctcatcgctc
     3241 gagcaggccg agcagcgcgg ccgccgcttc ctgctcggcg gcgcggcgcg aaccgccctt
     3301 gccgcgcgcg cgcaggccga gctcctcggc gacgcactcc acttcgaagg tctgctggtg
     3361 cgccgcgccg agcgtggcga cgacggcgta gcggggcagg ccgtggcggc gcgcctgcag
     3421 cgcctcctgc aggcgcgtct tggcgtcctt cgccgcgccg ccctcgcccg caccctcgag
     3481 cgccgcgccc agcgtgccga gcactgcgcg ccgcgcgccg tcgtagccgg catccaggaa
     3541 caccgcgccg tacagcgctt cgagcgcatc ggcgaggatc gagggccgct gcgcgccgcc
     3601 gctcgcgctt tcgccctgtc ccagccgcag ctgcgccgcc agcccgagct cgccggcgag
     3661 cgccgccagc gactcctcgc gcaccaggct ggcgcgcagg cgcgacagtt cgccttcgct
     3721 catcgcgggg tggcgcgcca gcagggcttc ggccaccacg cagcccagca cgccgtcacc
     3781 gagaaactcc agccgctcgt tgtgcggcga tccgtggctg cggtgggtca gcgcctgctc
     3841 gagaagctcg cggcgggcaa aacgatggcc gaggcgatcc tcgagggtgg cggtgcggga
     3901 caagcggcgc gcgcggcccc tgctagttgc tgctggtggc ggtgaaatcg atgtacaccc
     3961 cgatgttgtg gaacaggtgc acttccttgc ggtaggcggc ggtcaccgtg aagccgtcgc
     4021 cttccttgtt gatctccagg tcgctcgcct tgacggccgt gaaatcgtcg atcgcggcgc
     4081 gcgcgtcgaa agcgcgacgc aggtcgctga ccgcgctgcc gcggccgcgg ctttccagcg
     4141 tgatgccgtt gaccgccttc ttgatggcga agtactcgat gtacgcaggc agcagcttca
     4201 tcgcaagcag cgccgcgaac agcaccacca ccgagccgat gagcagcccc atcagggtaa
     4261 tcccgcgttg cctgttgcgc atagccaacc tcctgtcgga accggcgctc agcggaaggc
     4321 gccgaagcgc cccagctcgc tgaaattgag ccagatgaag aacgccttgc cgacgatgtt
     4381 ctcgtcgggt acgaagcccc acacgcggct gtcgctggaa ttgtcccggt tgtcgcccat
     4441 cacgaaatag tggccgggcg gcacggtgca cgccaggccc gacatattgt agttgcaatt
     4501 gccggaatag gggaacgcgc gcgcctgcgg cacgaacacc ggcgcgtcgt cctcgatcat
     4561 gacctggtgc tcggtgccgt tcagcgtctc caggtagcgg cgcgagtact gcatgcgctc
     4621 gcgcgacaag tagtcctcga tctgcttcac cggcaccgcc tggccgttga tggtcaggcg
     4681 cttgttgcgg tactcgacgc ggtccccggg caggccgacc acgcgcttga tgtagtcgag
     4741 cgagctgtcg tccgggtagc ggaacaccat cacgtcgccg cgctgcgggt cgccgatctg
     4801 gatcagcttg cggttcgcca ccggcaggcg gatgccgtag gcgaacttgt tgaccaggat
     4861 gaagtcgccg accagcagcg tggggatcat ggagcccgag ggaatcttga acggttccac
     4921 caggaaggag cgcagcagga acacgatcag gatcaccggg aagaagctca ccgagtactc
     4981 gacccaccag ggctgcctcg cctcggccgg gcggcgccgc gccagccagg cggcctccgc
     5041 cgcccacacg ccgccggtga ccagcagcag gaccagcagg aacagggcga agttcatgcc
     5101 tagtccgcgt cccgctgcag caccgccagg aaggcctcct gcgggatctc caccgagccc
     5161 acctgcttca tgcgcttctt gcccgccttc tgcttctcca gcagcttgcg tttgcgcgtg
     5221 acgtcgccgc cgtagcactt ggccagcacg ttcttgcgca gcgccttcac gttctcgcgc
     5281 gcgatgatgt tggcgccgat cgccgcctgg atggcgacgt cgaacatctg ccgcgggatc
     5341 aggccgcgca ggcgcgccgc cagttcgcgg ccgcggcgct gcgcgacttc gcggtgcacc
     5401 atgctggaga gcgcatccac gcgttcgccg ttgaccagca cgtcgaggcg gatcacgtcc
     5461 gccggacggt aggccttgaa ctcgtagtcc atcgagccga agccgcgcgt cagcgatttc
     5521 aggcggtcga agaaatccag caccacctcg ttcagcggca agtcgtagga caggaccacg
     5581 tggcgcgccg agtagtgcag gttggtctgc gtgccgcgct tctcggtgca cagggtcatc
     5641 accggcccga cgtactcctg cggcacgaac aaggtggtgg cgatcatcgg ctcgcggatc
     5701 tcggcgatca gcgacgggtc gggcagcttg gccgggttgg acaccagttg cacgccgccg
     5761 tcgcgcaggt gcacttcgta caccaccgag ggggccgtgg tgaccagttc catgccgtac
     5821 tcgcgttcca ggcgttcctg cacgatgtcc atgtgcagca ggccgaggaa gccgcagcg
//
LOCUS       DASRIE010000092        14106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1923938, whole genome shotgun sequence.
ACCESSION   DASRIE010000092 DASRIE010000000
VERSION     DASRIE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923938"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VFB53_04200"
     CDS             <1..1424
                     /locus_tag="VFB53_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN85970.1"
                     /translation="RFEILRTSSLKYYLGGTTGNPVPFYPIHLTGKDGKPNHSMVAAY
                     AALTGKTVNIADAYTADGFDFTGTRNFDKKTGYRSKSFLTVPMANHEHEIIGVLQLIN
                     AQDPKTGEILPFSNADQRLAESLASQAAIALTNRMLINQLEQLFESFINLINSAIDEK
                     SPYTGGHCQRVPALTMMLGEAASETERGPLSDFHMSEQDRYELKIAGLLHDCGKVTTP
                     VHVVDKATKLETIYDRIHLIDTRFEILKRDAEIAMLKDGGSPEARARYRERLRQFEED
                     RMFLRATNIGGERMHDDDIERVKRIAGYTWTDISGHEAKFLSDDELKNLTIRAGTLTA
                     EERQVINHHIVATIKMLEALPWPRHLKNVPEYAGGHHERMDGKGYPRGLKREQMSVQA
                     RVMGIADIFEALTAKDRPYKKGKTLSESLEILGRFSQNGHVDPDLFDVFVRRKVYLRY
                     AEMFLDKEQIDEVDESKIPGYAP"
     gene            1593..2069
                     /locus_tag="VFB53_04205"
     CDS             1593..2069
                     /locus_tag="VFB53_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZN85971.1"
                     /translation="MKRLQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKMSEVL
                     LAMSSCRTSITEVYQTGGTPPGGSNWGCEGGNLSKYVGAIATDDNGKVTSTVQAISPL
                     VDGGLVTLVPLVTDANTPAVAANDMGKGLYGWRCGNSATDGTTVSPKYLPGSCRGT"
     gene            2136..3731
                     /locus_tag="VFB53_04210"
     CDS             2136..3731
                     /locus_tag="VFB53_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HZN85972.1"
                     /translation="MLTWIRSKLGTAGRTPAQLLSQAEAAYAAGAPDQARRLCLALLR
                     RAPRDGRALVLLAAIAADAGQIDEGLQWARRAQDADPGAASPHYVMGRLWQEAARLPD
                     AEASYRSAIGLQPDHARAHNNLGCVLHMQGRLQDALVAYRRALDLDPALPQANQNYAA
                     IVRDAGALERAAAAYLRHTAAHPEDAMAFNDLGNAYRELGRHQDALAAYERALALDPD
                     LAQAHFSRSFVLLLLGDYSAGWRDYEWRWRINAFNGPMLRFSQPIWDGGRIEAGALLL
                     HAEQGLGDTLQFVRYASRAAERCESVILECQPELVALLRSTAGLARVLAKGEALPPFA
                     RHAPLMSLPALFGTTLADIPWSGPYVRPDPVRVAAWRDAIAGPGLKVGLVWAGRPQQW
                     DDRKRSISLQQLAPLARVPDVAFYSLQVGVAAAQAARPPESMRLVDVAARFKDFSDTA
                     AIVASLDLVITIDTSVAHLAGAMGVPVWVLVAQAPDWRYHLGRSDNPWYPTMRLFRQE
                     RDGDWSGAIEQVAAALGQRAAQS"
     gene            complement(3728..5377)
                     /locus_tag="VFB53_04215"
     CDS             complement(3728..5377)
                     /locus_tag="VFB53_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN85973.1"
                     /translation="MSRAAAWQLGGACAITLLAAALYLPFLGNPLVFDDRIFFSGRLF
                     SYYATHPLGLDLRLPANFSLAATEVLLGGIHAQRAVSLILHIGTALVLYRLLLDLQAT
                     ALPHQPREDGALRAAFGAAAFALHPVAVYGAAYLVQRSTVLATLFSLLSALLFLRGMR
                     RGSYADALSAALCYALAVLSKEHAILLPAAVLPMMALARPARRFALPYAAIYLAACAP
                     AALFVAMRSLGVIGGVYEEAFGAIAAQIETASGEDISGQPLALSAVTQAGLFFKYLAL
                     WFWPDTGAMAFDLRIDFLASWSVGWIALKGLGFAACGILGACLALRGGAAGIAGVGVL
                     YAWLLFFVEFSAARFQEPFVLYRSYLWGPGIACVAVAALAPVTRIAAIATGVAAMGAL
                     AIGAQDRLTTFSSPLRLWEDAAAKLPDKPVPWGSRTLYGVGREYLYSGQTEKAIAAAD
                     RCIRLYPRTAQCYYARGAIHLHLEEFESARGALSRALELKPSDGVTAHRLGLALEGLD
                     RVEEAKAAYRRAMELGFYAATFELQRLESPGSGLLAPEMRR"
     gene            complement(5374..6606)
                     /locus_tag="VFB53_04220"
     CDS             complement(5374..6606)
                     /locus_tag="VFB53_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN85974.1"
                     /translation="MSLGALLKVALRALAVNKLRSALTMLGIIIGVAAVIVMVAVGAG
                     AQARVEEQIRSLGSNLLLILSGARTQSGVRLGTGSNYTLSEDDAIAISREVPDALAAP
                     ALRGGAQVIWGNANWATQLYGTTPEYLDVRQWPLAAGRSFEPAELTGAGKVCLVGQTV
                     VRQLFGSTDPLGQVIRIKRVPFTVVGVLETKGQSMMGTDQDDLIVLPIATARGRVLGS
                     ANLAKQRAVSTIWVKVADGADTKTVEEQVRLLLRQRHRLQPGADDDFSLRNLAEVMAA
                     QEASSRVLALLLAAVASVSLVVGGIGIMNIMLVSVTERTREIGLRMALGARTRDILGQ
                     FLVEAVTLSLIGGLVGVALGSGAALAIAQFAGWRIVLSPDAVLLAVAFAFAIGVFFGY
                     YPARKAARLNPVEALRFE"
     gene            complement(6603..7340)
                     /locus_tag="VFB53_04225"
     CDS             complement(6603..7340)
                     /locus_tag="VFB53_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN85975.1"
                     /translation="MTEALIRTRDLAKVYRVGDNDIHALAGVSVAIERGESLAVMGPS
                     GSGKSTFMNLLGCLDRPSAGEYLLEGQRVSDLAGDALAAVRNRNIGFVFQNFNLLART
                     SALENVELPLVYAGVAASERRTRALEMLERVGLAARAGHLPQQLSGGQQQRVAIARAL
                     VTRPVLILADEPTGALDSRTSLEIMALFQELNRQGITLLLVTHEADVARFARRVLRFR
                     DGRVIADEGNAAAEDAQRLLQGEALPA"
     gene            complement(7340..8887)
                     /locus_tag="VFB53_04230"
     CDS             complement(7340..8887)
                     /locus_tag="VFB53_04230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZN85976.1"
                     /translation="MKRAMLKVALAVAVLFGAAAGYLWLQQRDGAEGASYRLAKLERG
                     PLTAVVAASGTLNAVTTVQVGSQISGQVKEIHADFNSAVKQDQVIARIDPATYELRVN
                     QARADLDAAHSAVAVARSGLAAQQAEVSRVKVTLAEALRDLERKKSLVDRNYISGAEL
                     DKSRSLAEATREQLKSVEAQIQVSEAQVRSAQAAVKQRESLLKQAQVDLERTIIRAPV
                     DGTVILRNVDAGQTVAASLQAPVLFTIARDLRDMQVEAAIDEADVGRLRAGQAASFTV
                     DAFPRRSFAGEIVQIRKSPVNVQNVISYTVVISARNPDLSLLPGMTANVRIVVEHRDS
                     ALKAPNAALRFRPAGTPEQKPAAEAAQAPGAQALQQLRQRLLAEVKPDAAQQARLEEI
                     FAELRGKLAQLREESDEAARRRLAERARAEMRTRIAEILRPEQKAAYERLLRESGGRG
                     TAAAGRVWVLQAGQPKSVDVRLGLSDGSSTELLGSTLAEGTEVIVGLAASAEKAPGGG
                     GPRPRLF"
     gene            complement(8904..9407)
                     /gene="moaC"
                     /locus_tag="VFB53_04235"
     CDS             complement(8904..9407)
                     /gene="moaC"
                     /locus_tag="VFB53_04235"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZN85977.1"
                     /translation="MARKPKAKLKLSHFDGRGRAHMVDVGAKARTHRVAVASGRICMS
                     KATLRLLAAGRAAKGDVLGVARVAAIQAAKRTPELIPLAHPIAITRAAVEFALDARRG
                     AVGVVATVECRGSTGVEMEALCAAAVGLLTVYDMLKAVDRGMVLEALRLEEKRGGRSG
                     TWRRGGA"
     gene            9450..10898
                     /locus_tag="VFB53_04240"
     CDS             9450..10898
                     /locus_tag="VFB53_04240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN85978.1"
                     /translation="MGRTFVLLAALAAALASGSAPAQPLPELGDAASAAISPQVERRI
                     GESIAREIRYRDPAYVDDAEIADYLEQLGRRLLAAVPGARQDFEFFAVRDGAINAFAL
                     PGGYIGVHTGLLVAAASESEVASVLAHEIAHVTQRHIARLIGAQQQMQLPGMVALAAA
                     ILLGGSRPDLAGGAVAAVQGGMVQQQLSYTRDFEREADRVGFGTLAAAGFDVRAMPAF
                     FEKMQRGSRLADDGSVPSYLRSHPMTTERIADAQNRAADAPYRQHLDSVDFHLVRAKL
                     RAEAGEARDAVASLAAAVKERRYANEAAARYGLVLARLRARQVREAQAELALLRAGGA
                     ASPMIETLAARVQQAAGDAAGALDTLRQALRRYPGRLPLVYAYAEGLQQAGRSRESLA
                     ALEEPLRRYPGDARLRTLQARTYAALGKRLLQHQAQAELYVLRGSVPAAIEQLQIAQA
                     AGDGNFYELSAVEARLKELRRQHAQDLRDAKR"
     gene            complement(10899..11891)
                     /locus_tag="VFB53_04245"
     CDS             complement(10899..11891)
                     /locus_tag="VFB53_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZN85979.1"
                     /translation="MEPERTASANDTPAAGSRWLVTVTQLPTEDPAGRMRMLRTLESL
                     GAAVMREGVYVLPDSADNRNALEHLARYVTQNAGVASVLRVVAADAAQQSQFERYFDR
                     SARYDGLTKIIHSLQVGFGVSDASAISRVLHKQRREFEAIAALDFFPTEARGRTEQAL
                     AEAEAAVRQLLFPAQAGAGVKADEPMLGRTWATRRPLWADRLACAWLIRRFLDAEAKL
                     QWLDKGATCPPEAIGFAFEGARFANSDARITFEQMLAQFGLGAHAALARIGAIVHYLE
                     VRGTPVPEAAGVQTLLQGAVRRAAGDDELLREAEKTFDLLYDAYSDAREVTRAE"
     gene            11945..12409
                     /locus_tag="VFB53_04250"
     CDS             11945..12409
                     /locus_tag="VFB53_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85980.1"
                     /translation="MAQAARWMRTHISGERRPASAGADLAAAQSAAAEPIVLWIYGLR
                     ESAFALIAPRRLAPGRARRWAAWGAMPAVATCRQYGLPAYLQDAAIWLHGRLVASVDA
                     ARVGECALIESRFPGPLAAVPGLEAAFRARIEAQHGWRFDTAWPTAEEAQRC"
     gene            12403..13035
                     /locus_tag="VFB53_04255"
     CDS             12403..13035
                     /locus_tag="VFB53_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85981.1"
                     /translation="MLSRRAMLQGAAALLLVRDALAAGGVEEGIYRVRGEARVNDARA
                     RQGMRVRPGDAVATLAGGEMVFVINRDAFLLRANSRLEVGSAAADVFRVVTGALLSVF
                     QPGGRKTLRTPTATIGIRGTGIYVESEADRTYACTCYGEAELVPNDEPAARETVKTTH
                     HDQPRYIMAKGAPQMIMRAPVINHTDAELILLESLVGRSVPFDPFGQTYK"
     gene            complement(13026..>14106)
                     /locus_tag="VFB53_04260"
     CDS             complement(13026..>14106)
                     /locus_tag="VFB53_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85982.1"
                     /translation="VLAPMAGVWVDRLSKTTVLRATNSVALAQSLAMLALVASGQIQP
                     WHLIAGNLVLGIVNAFDSPARQSFLIELVAGREDLPNAIAFQSMLMNGARFVGPMVGG
                     AVIALGGEVWGFGLNSASYVAILAALAAVQVQARPPESAHQGWLAQLAAGFRWAYGFL
                     PSRSILLLLAAVSVGTQPLQALAPFFARDVFGGNSQTLGWMIGAGGLGAVSGMLLLAL
                     RPTVRGLLTVIGWAGAAAGIAVIAFAWCPWFWPALALTYAAGMGMMLTAGSINTILQT
                     IVEDRLRARVAALYIMSFLGMSPLGALAAGWAALYVGPPAALAIGGALALCASLAYLA
                     RLPAIRREIRPVYERLGVLPRAHHL"
BASE COUNT         2183 a         4986 c         5023 g         1914 t
ORIGIN      
        1 tgcgcttcga gatcctgcgc acctcgagcc tcaagtacta cctgggcggc accaccggca
       61 acccggtgcc gttctacccg atccacctca cgggcaagga cggcaagccg aaccacagca
      121 tggtggcggc ctacgcggcg ctcaccggca agacggtgaa catcgccgac gcctacaccg
      181 ccgacggctt cgacttcacc ggcacgcgca atttcgacaa gaagaccggc taccgctcga
      241 agtccttcct caccgtgccg atggcgaacc acgagcacga gatcatcggc gtgctgcagc
      301 tgatcaacgc gcaggatccc aagaccggcg agatcctgcc gttctccaac gccgaccagc
      361 gcctggcgga gtcgctggcc tcgcaggcag cgatcgcgct caccaatcgc atgctgatca
      421 accagctcga gcagctgttc gagtcgttca tcaacctgat caactcggcg atcgacgaga
      481 agtcgcccta caccggcggc cactgccagc gggtgccggc gctcaccatg atgctggggg
      541 aggctgcgag cgagaccgag cgcggcccgc tcagcgactt ccacatgagc gagcaggacc
      601 gctacgagct gaagatcgcc gggctgctgc acgactgcgg caaggtcacc acgccggtgc
      661 acgtcgtcga caaggccacc aagctggaga ccatctacga ccgcatccac ctcatcgaca
      721 cgcgcttcga gatcctgaag cgcgacgccg agatcgccat gctcaaggac ggcggcagcc
      781 ccgaggcgcg cgcgcgctac cgcgagcgcc tgcgccagtt cgaggaggac cgcatgttcc
      841 tgcgcgccac caacatcggc ggcgagcgca tgcacgacga cgacatcgag cgcgtgaagc
      901 gcatcgccgg ctacacctgg accgacatct ccggccacga ggccaagttc ctcagcgacg
      961 acgagttgaa gaacctgacc atccgcgcgg gcacgctcac cgccgaggag cgccaggtga
     1021 tcaaccacca catcgtggcc accatcaaga tgctcgaggc gctgccctgg ccgcgccacc
     1081 tgaagaacgt gccggagtac gccggcgggc accacgagcg catggacggc aagggctatc
     1141 cgcgcggcct gaagcgcgag cagatgtcgg tgcaggcgcg cgtcatgggc atcgccgaca
     1201 ttttcgaggc gctgaccgcc aaggaccggc cctacaagaa gggcaagacg ctgtccgagt
     1261 cgctggagat cctcggccgc ttcagccaga acggccacgt cgaccccgac ctgttcgacg
     1321 tctttgtgcg ccgcaaggtg tacctgcgct acgccgagat gttcctcgac aaggagcaga
     1381 tcgacgaggt cgacgagagc aagatcccgg gctacgctcc ctgatctccc gggggcgcat
     1441 gtgacagaaa ctgtcagcta tcgaccaaaa ttgccagggc gggggatcat gacccgccat
     1501 ctgcatgaaa aagtgcactg aaagtgcctg gcatggggct tgctttctac ctgcttggca
     1561 tccatgccaa acttagagca aaggagaact agatgaaacg cctgcaacaa ggctttacgc
     1621 tgatcgagtt gatgatcgtg gttgcgatca tcggcatcct ggcagcggtc gcgctccccg
     1681 cgtaccagga ctacaccatc cgggcgaaga tgtcggaagt gctgctggcc atgagctctt
     1741 gccgcacctc catcaccgag gtgtaccaga cgggcggcac gcctcccgga ggctcgaact
     1801 ggggctgcga aggcggcaac ctgtcgaaat acgtgggcgc aatcgccacc gacgacaacg
     1861 gcaaggtgac gtcgacggtt caggcgatct cccccctggt cgatggcggc ctggtgacgc
     1921 tggttcccct ggtcacggac gccaatacgc cggcggtggc tgccaacgac atgggcaagg
     1981 gcctgtacgg ctggcgctgc ggcaattcgg cgaccgacgg tacgaccgtg agcccgaagt
     2041 acctgccggg ctcctgccgc ggtacctgat ccagaagttt tcggcaaaag aaaaaggcgc
     2101 ccagtgggcg ccttttttct ttggcatcat gcctgatgct gacctggata cgttcgaagc
     2161 tcggcaccgc ggggcgaacc cccgcgcaac tcctgtccca agcggaggct gcctatgcgg
     2221 cgggagcccc ggaccaggcg cggcggcttt gcctggcgct gctgcgtcgc gcgccacgcg
     2281 acgggcgcgc gcttgtgctg ctggcggcga tcgcggcgga cgcggggcaa atcgacgagg
     2341 gcctgcaatg ggcgcgacgc gcgcaggacg ccgacccggg cgccgcctcg ccgcactatg
     2401 tcatggggcg gctgtggcag gaggcggcgc gtttgcctga cgcggaagcg agctaccgca
     2461 gcgcgatcgg cctgcagccc gatcacgcgc gcgcgcacaa caacctcggc tgcgtcctgc
     2521 acatgcaagg ccggctgcag gacgcgctgg tggcctaccg cagggcgctc gacctggatc
     2581 cggcgctgcc gcaggccaat cagaactacg ccgcgatcgt acgcgatgcg ggcgcgctcg
     2641 agcgtgcggc ggcggcctat ctccggcaca ccgcggccca cccggaggac gccatggcgt
     2701 tcaacgatct gggcaacgcc taccgcgagc tcgggcggca ccaggatgcg ctggcggcgt
     2761 acgagcgcgc actggcgctc gaccccgacc tcgcgcaggc gcacttcagc cgctctttcg
     2821 ttctgctgct gctcggcgac tacagcgcgg gttggcgcga ctacgaatgg cggtggcgca
     2881 tcaatgcctt caatggcccg atgctccggt tttcccagcc gatctgggac ggcggccgca
     2941 tcgaggcagg cgcgctgctg ctgcatgcgg aacagggcct cggcgacacc ttgcagttcg
     3001 tgcgctacgc ctcgcgtgcc gctgagcgat gcgagtcggt gatcctggaa tgccagccgg
     3061 agctggtagc gctgctgcgg agtacggcgg gcctggcgcg ggtgctggcg aaaggcgagg
     3121 ccttgccgcc cttcgccagg catgccccgc tcatgagcct tccagcgctc ttcggtacca
     3181 cgctggccga cattccctgg agcggtccct acgtgcgccc ggacccggta cgggtcgcgg
     3241 cgtggcgcga cgccatcgcc gggcccggac tcaaggtcgg gctggtctgg gccggccggc
     3301 cgcagcagtg ggacgaccgc aagcgctcga tttcgctcca gcagctggcg cccttggcgc
     3361 gcgtcccgga cgtcgccttc tacagcctgc aagtcggcgt cgcggcggcg caagccgcac
     3421 ggccgcccga gtccatgcgc cttgtcgacg tggcggctcg cttcaaggat ttttccgata
     3481 ccgcggcaat cgtcgccagc ctcgacctcg tgatcacgat cgatacttcg gtggcgcacc
     3541 tggcgggcgc gatgggcgtg ccggtgtggg tgctggtcgc gcaggcgccg gactggcgct
     3601 accacctggg gcgcagcgac aatccctggt acccgacgat gcgcctgttc cgccaggagc
     3661 gcgacggcga ctggagcggc gcgatcgagc aggtcgccgc ggcgctgggg cagcgcgctg
     3721 cgcagagtta acgacgcatt tccggagcga gcaggccgct gccgggcgat tccagccttt
     3781 gcagctcgaa tgttgcagca tagaagccga gctccatcgc gcgccggtag gcggccttgg
     3841 cttcttcgac gcggtccaat ccctccagcg ccaggccgag gcggtgggcc gtgacgccgt
     3901 cgctgggctt cagttccagg gcgcgcgaca gggcgccccg cgccgattcg aactcctcca
     3961 ggtgcaggtg aatcgccccg cgggcgtagt agcactgcgc tgtccgaggg tagagccgga
     4021 tgcagcgatc ggccgccgcg atcgccttct ccgtctgccc gctatagagg tactcccggc
     4081 ctacgccgta aagggtgcgc gagccccagg ggaccggctt gtcgggcagc ttggccgccg
     4141 cgtcctccca caggcgcagg ggactggaga aggtcgtcag ccgatcctgc gcgccaatgg
     4201 caagtgcccc cattgccgca actcccgtgg cgatcgcggc tatgcgggtt acgggagcca
     4261 gagccgccac ggcaacgcag gcgatgccgg ggccccagag gtagctgcgg tagagcacga
     4321 agggctcctg gaacctggcg gcgctgaact cgacgaaaaa caggagccag gcgtagagca
     4381 cacccacgcc ggcgatgccg gccgcgccgc cgcgcagcgc caggcaggcg cccaggatgc
     4441 cgcaagcggc gaatcccaga cccttgagcg caatccagcc gaccgaccat gacgcgagga
     4501 aatcgatgcg caggtcgaag gccatggcgc cggtgtccgg ccagaaccag agcgccaggt
     4561 acttgaagaa aagtcccgcc tgcgtgaccg cgctcagcgc gagcggctgc ccggagatgt
     4621 cctcgccaga ggcggtctcg atctgcgcgg cgatggcgcc gaacgcttcc tcgtaaaccc
     4681 cgccgatcac gcccaggctg cgcatggcaa cgaacagcgc ggcgggcgcg cacgccgcga
     4741 ggtagatcgc ggcatacggc agggcgaagc gccgcgcggg gcgcgccagc gccatcatcg
     4801 gcagcacggc ggccggcagc aggatggcgt gctccttgga caggaccgcc agcgcatagc
     4861 acagcgcggc ggatagcgca tcggcatagc tgccgcgacg catgccgcgc aggaacaaca
     4921 gcgcggacag cagggaaaac agggtcgcca ggacagtgct gcgctggacc aggtacgcgg
     4981 cgccgtagac cgccaccgga tgcagggcga aagccgcggc accgaaggcc gcgcgcaggg
     5041 cgccgtcctc gcgaggctgg tgcgggaggg cggtggcctg caggtcgagc agcaaccggt
     5101 aaaggacgag ggcggtgccg atatgcagga tcaggctgac cgcgcgctgc gcgtggatgc
     5161 cgcccagcag cacctcggtg gcggcgagcg agaagttcgc cggcagcctc aggtcgaggc
     5221 cgagcggatg cgtggcgtag taggagaaca ggcgcccgga aaagaagatg cggtcgtcga
     5281 acaccagggg attgccgagg aacggcaggt acagcgctgc cgcgagcagc gtgatggcgc
     5341 aggccccgcc cagctgccag gcggcggctc ggctcattcg aagcgcagcg cttccacggg
     5401 gttgaggcgc gccgccttgc gcgccgggta gtagccgaag aacacgccga tggcgaaggc
     5461 gaaggccacc gcgagcagca cggcatcggg cgacagcacg atgcgccagc cggcgaactg
     5521 cgcgatggcc agcgcagccc ccgagcccag ggccacgccg accaggccgc cgatcagcga
     5581 cagggtcacc gcttcgacga ggaactggcc gaggatgtcg cgcgtgcgcg cgccgagcgc
     5641 catgcgcagg ccgatctccc gggtgcgctc ggtgaccgac accagcatga tgttcatgat
     5701 gccgatgccg cccaccacca gcgacaccga tgcgaccgct gccagcagca gcgcgagcac
     5761 gcggctggag gcttcctgcg ccgccatcac ctccgccagg ttgcgcagcg agaaatcgtc
     5821 gtcggcgccc ggttgcagcc ggtggcgctg gcgcagcagc aggcgcacct gctcctccac
     5881 cgtcttggtg tccgcgccgt cggcgacctt gacccagatg gtgctcaccg cgcgctgctt
     5941 ggcgaggttg gccgagccga gcacgcggcc gcgcgcggtg gcgatcggca ggacgatcag
     6001 gtcgtcctgg tcggtgccca tcatgctctg gcccttggtc tccagcacgc ccaccacggt
     6061 gaacggcacg cgcttgatgc ggatcacctg cccgagcgga tcggtactgc cgaacaactg
     6121 gcgcaccacg gtctgcccga ccaggcagac cttgcccgcg ccggtcagct ccgcgggctc
     6181 gaaggagcgc ccggcggcga gcggccactg gcgcacgtcg aggtactcgg gcgtggtgcc
     6241 gtagagctgc gtggcccagt tggcattgcc ccagatcacc tgcgcgccgc cgcgcagcgc
     6301 gggcgcggcg agcgcgtccg gcacctcgcg gctgatggcg atggcatcgt cctccgacag
     6361 ggtgtagttg gagcccgtgc cgaggcgcac gccgctctgc gtgcgcgcgc ccgacaggat
     6421 gaggagcagg ttggagccca gcgagcggat ctgctcctcg acgcgggcct gcgcgccggc
     6481 gccgacggcg accatcacga tcaccgccgc caccccgatg atgattccaa gcatggtgag
     6541 cgcgctgcgc agcttgttca ccgccagcgc gcgcaacgcc accttgagga gcgccccgag
     6601 gctcatgccg gcagcgcctc gccctgcagc agccgctgcg cgtcctcggc cgccgcattg
     6661 ccctcgtcgg cgatcacccg gccgtcgcgg aagcgcagca cgcggcgcgc gaagcgcgcc
     6721 acgtcggcct cgtgcgtcac cagcagcagg gtgatgccct ggcggttgag ctcctggaac
     6781 agcgccatga tctccaggct ggtgcgcgag tcgagcgcgc cggtgggctc gtcggcgagg
     6841 atcagcaccg gccgcgtcac cagcgcgcgg gcgatcgcca cgcgctgctg ctggccgccg
     6901 gagagctgct gcggcaggtg gccggcgcgc gccgcgaggc cgacgcgctc gagcatctcg
     6961 agggcgcggg tgcggcgctc ggaggccgcc acgccggcat agacgagggg aagctcgacg
     7021 ttctccagcg cgctggtgcg cgccagcagg ttgaaattct ggaacacgaa gccgatgttg
     7081 cggttgcgca ccgcggccag cgcgtcgcct gcgaggtccg acacgcgctg cccctcgagc
     7141 aggtactcgc cggcgctcgg ccggtcgagg catccgagca ggttcatgaa ggtcgacttg
     7201 ccggagcccg atgggcccat gaccgccagg ctctcgccgc gctcgatcgc cacgctcaca
     7261 ccggcgaggg cgtggatgtc gttgtcgccg acccggtaga ccttcgccag gtcgcgcgtg
     7321 cggatcagcg cttccgtcat cagaacagcc gcgggcgcgg cccgcccccg cccggggcct
     7381 tctccgcgct ggcggcgagg ccgacgatga cctcggttcc ctcggcgagc gtgctgccga
     7441 ggagctcggt gctgctgccg tcgctcaggc ccagccgcac gtcgacggat ttcggctgtc
     7501 ccgcctgcag cacccacacg cgccccgcgg ccgccgtccc gcgcccgccc gactcgcgca
     7561 gcagccgctc gtaggcggct ttctgctccg gacgcaggat ttccgcgatc cgggtgcgca
     7621 tctcggcgcg cgcacgctcg gcgaggcggc ggcgcgccgc ctcgtccgat tcctcgcgca
     7681 gctgcgcgag cttgccgcgc agctcggcga agatctcctc cagccgcgcc tgctgcgcgg
     7741 catcgggctt cacctcggca agcagccgct ggcggagctg ctgcagcgcc tgcgcgcccg
     7801 gcgcctgcgc ggcctccgcc gcgggcttct gttcgggcgt gcccgcgggc cggaatcgca
     7861 gcgccgcgtt gggcgccttg agcgcgctgt cgcggtgctc gaccacgatg cgcacgttgg
     7921 cggtcatgcc cggcagcagc gacaggtcgg gattgcgcgc cgagatcacc accgtgtagc
     7981 tgatcacgtt ctgcacgttg accggcgact tgcggatctg cacgatctcg ccggcaaagc
     8041 tgcggcgcgg gaatgcgtcc accgtgaagc tcgccgcctg gccggcgcgc aggcggccga
     8101 cgtcggcttc gtcgatcgcc gcctcgacct gcatgtcgcg caggtcccgc gcgatggtga
     8161 acagcaccgg cgcctgcagg ctcgccgcca ccgtctgccc ggcgtccacg ttgcgcagga
     8221 tcaccgtgcc gtccaccggg gcgcggatga tggtgcgctc caggtccacc tgcgcctgct
     8281 tgagcagaga ctcacgctgc ttcacggctg cctgcgcgct gcgcacctgc gcctcgctca
     8341 cctggatctg cgcctccacc gacttcagct gctcgcgcgt cgcctcggcc aggctgcgcg
     8401 acttgtcgag ctcggcgccc gagatgtagt tcctgtcgac cagggacttc ttgcgctcca
     8461 ggtcgcgcag cgcctccgcc agcgtcacct tgacgcggct cacttccgcc tgctgcgcgg
     8521 cgaggccgga gcgcgccacg gccaccgcgc tgtgcgcggc gtcgagatcc gcgcgcgcct
     8581 ggttgacgcg cagctcgtag gtcgcagggt cgatacgcgc gatgacctgg tcctgcttca
     8641 ccgcggaatt gaaatcggcg tggatctcct tcacctggcc cgagatctgc gagccgacct
     8701 gcaccgtggt cacggcgttg agggtgccgc tcgccgccac cacggcggta agcggtccgc
     8761 gctccagctt ggccaggcgg tagctggcgc cttcggcgcc atcgcgctgc tgcagccaca
     8821 ggtagccggc cgccgcgccg aacaggacgg cgacggcgag ggcgaccttg agcatggcgc
     8881 gtttcacggc agccgctcgc ttatcaagcg ccgccgcgcc gccaggtgcc cgagcggccg
     8941 ccgcgctttt cctccaggcg cagcgcctcc agcaccatgc cgcgatccac tgccttgagc
     9001 atgtcgtaca cggtcagcag tcccaccgcg gcggcgcaca gcgcctccat ttccaccccc
     9061 gtggacccgc ggcactccac ggtggcgacg acgccgaccg cgccgcggcg cgcatccagc
     9121 gcgaactcga cggcggcgcg cgtgatggcg atggggtggg cgagcgggat cagttccggg
     9181 gtgcgtttcg ccgcctggat cgcggcgacg cgcgccacgc ccagcacatc gcccttggcg
     9241 gcgcggcccg cggcgagcag gcgcagcgtc gccttggaca tgcagatgcg gccggaggcc
     9301 accgcgacgc ggtgcgtgcg cgccttggcg ccgacgtcca ccatgtgcgc gcgaccgcgg
     9361 ccgtcgaaat ggctcagctt gagtttcgct ttcggttttc ttgccatggc agcaccggaa
     9421 cttgcagggg gcgacggtat catatccgca tgggccgcac cttcgtcctg ctggccgcat
     9481 tggctgcggc gctggcatcg ggcagcgcgc cggcgcagcc gctgccggag ctgggcgacg
     9541 ccgcgagcgc ggcgatttcg ccgcaggtgg agcggcgcat cggcgagagc atcgcccggg
     9601 agatccgcta ccgcgacccg gcgtacgtcg acgatgcgga gatcgccgac tacctcgagc
     9661 aactcgggcg gcggctgctc gcggcggtgc cgggggcgcg ccaggatttc gagttcttcg
     9721 cggtgcgcga tggcgcgatc aacgccttcg cccttccggg gggctacatc ggcgtgcaca
     9781 ccgggctgct ggtcgcggcg gccagcgaat ccgaggtggc ctcggtgctg gcgcacgaga
     9841 tcgcgcacgt cacgcagcgt catatcgcgc ggctgatcgg cgcgcagcag cagatgcagc
     9901 tgcccggcat ggtggcgctg gccgccgcca ttctcctcgg cggctcgcgc ccggatctcg
     9961 caggcggggc ggtggcggcg gtgcagggcg ggatggtgca gcagcagctc agctacacgc
    10021 gcgacttcga gcgcgaggcc gaccgcgtcg gcttcggcac gctggccgcc gccggtttcg
    10081 acgtgcgcgc catgccggcg ttcttcgaga agatgcagcg cggctcgcgc ctggcagacg
    10141 acggcagcgt gccgagctac ctgcggtccc acccgatgac caccgagcgc atcgcggacg
    10201 cgcagaaccg ggcggcggac gcaccttacc gccagcacct cgacagcgtg gatttccacc
    10261 tggtgcgcgc caagctgcgc gccgaggcgg gcgaggcgcg cgatgccgtg gcgagcctcg
    10321 ccgccgcggt caaggagcgg cgctacgcca acgaagccgc ggcgcggtac ggcctggtgc
    10381 tggcgcgcct gcgagcgcgg caggtgcgcg aggcgcaggc cgagctcgcg ctgctgcgcg
    10441 ccggcggcgc ggcgagcccg atgatcgaga cgctggcggc gcgcgtgcag caggccgcgg
    10501 gcgatgcggc gggcgcgctc gacaccttgc gccaggcgct gcggcgctac ccggggcggc
    10561 tgccgctggt gtacgcctac gccgagggct tgcagcaggc ggggcgcagc cgggagtcgc
    10621 tcgccgccct cgaggagccg ctgcgccggt accccgggga cgcgcgcctg cgcacgctgc
    10681 aggccaggac ctatgccgcg ctcggcaagc ggctcctgca gcaccaggcg caggccgagc
    10741 tctacgtgct gcgcggcagc gtgccggcgg ccatcgagca gctgcagatc gcgcaggccg
    10801 cgggcgatgg caatttctac gagttgtcgg cggtggaagc ccggctcaag gaactgcgcc
    10861 gccagcacgc gcaggacctg cgcgacgcca agcgctgact attcggcgcg ggttacttcg
    10921 cgcgcatccg agtaggcgtc gtacagcaga tcgaaagtct tctcggcctc gcgcagcaac
    10981 tcgtcgtcgc cggccgcgcg ccgcacggcg ccctgcagca gcgtctgcac gccggcggct
    11041 tcgggcaccg gcgtgccgcg cacttcgagg taatgcacga tggcgccgat gcgcgcgagc
    11101 gcggcatgcg cgccgaggcc gaactgcgcc agcatctgct cgaaggtgat gcgcgcgtcg
    11161 ctgttggcga agcgcgcgcc ctcgaaggcg aagccgatgg cctccggcgg gcaggtggcg
    11221 cccttgtcca gccactgcag cttggcctcg gcgtcgagaa agcgccggat cagccaggcg
    11281 caggcgagcc ggtcggccca cagcgggcgg cgcgtggccc aggtgcggcc gagcatgggt
    11341 tcgtcggcct tcacgccggc gcccgcctgc gccgggaaca gcagctggcg caccgcggcc
    11401 tcggcctcgg ccagcgcctg ctcggtgcgc ccgcgcgcct cggtgggaaa gaaatccagc
    11461 gcggcgatcg cctcgaactc acgccgctgc ttgtgcagca cgcgcgagat cgccgaggcg
    11521 tcggaaacgc cgaacccgac ctgcaggctg tggatgatct tggtgagccc gtcgtagcgc
    11581 gccgagcggt cgaaatagcg ctcgaactgg gactgctgcg ccgcgtccgc cgccaccacc
    11641 cgcaggacgc tggcgacgcc ggcgttctgc gtcacgtagc gtgcgaggtg ctcgagcgca
    11701 ttgcggttgt cggccgagtc gggcagcacg tagacgccct cgcgcatcac cgccgcgccc
    11761 agcgattcga gcgtgcgcag catgcgcatg cggccggccg ggtcctctgt gggcagctgc
    11821 gtgacggtca ccagccagcg actgcccgcg gccggcgtgt cgttcgcgga cgcggtgcgt
    11881 tccggttcca tggcgcgcgg cgattattgc acagggcggc gcgcctgata tataagcctg
    11941 aatcatggcg caggcagcgc gttggatgcg aacgcacatc tcgggcgagc gacggccggc
    12001 gtcggcgggc gcggacctgg ccgcggcaca gagcgccgcc gccgagccga tcgtgttgtg
    12061 gatttacggc ctgcgcgaga gcgccttcgc gctgatcgcg ccgcggcgcc tggcgcccgg
    12121 gcgcgcgcgg cgctgggcgg catggggggc catgccggcg gtcgccacct gccggcagta
    12181 cggcctgccc gcctacctgc aggacgcggc gatctggctg cacggcaggc tggtggccag
    12241 cgtcgacgcg gcgcgggtcg gcgaatgcgc gctcatcgaa tcgcgcttcc ccggacccct
    12301 ggcggccgtg ccggggctgg aggcggcgtt ccgggcgcgc atcgaggcgc agcacggctg
    12361 gcgcttcgac acggcctggc cgacggcgga ggaagcgcag cgatgctgag caggcgtgcg
    12421 atgctgcagg gcgccgcggc cctgctgctc gtgcgcgatg cgctcgccgc gggcggcgtg
    12481 gaggagggca tctaccgcgt gcgcggcgag gcgcgcgtca acgacgcgag ggcacggcag
    12541 gggatgcggg tgcggcccgg cgacgcggtg gcgacactgg cgggcggcga gatggtgttc
    12601 gtgatcaacc gcgacgcctt cctgctgcgg gcaaattcgc gcctcgaagt gggcagcgcc
    12661 gcggcggacg tgttccgcgt cgtcaccggt gcgctgctgt cggtattcca gccggggggg
    12721 cgcaagaccc tgcgcacgcc gaccgccacc atcggcatcc gcggcaccgg catctacgtc
    12781 gaaagcgagg cggaccggac ctacgcctgc acctgctacg gcgaggcgga gctggtaccg
    12841 aacgacgaac cggcggcgcg cgaaacggtg aagaccacgc accacgacca gccgcgctac
    12901 atcatggcga agggcgcgcc gcagatgatc atgcgcgcgc cggtgatcaa tcacaccgac
    12961 gcggaattga tactgctcga atcgctggtc ggccgcagcg tgccgttcga tcccttcggc
    13021 cagacctaca agtgatgcgc gcgcggcagg acgccgagcc gctcgtagac ggggcggatc
    13081 tcgcggcgga tggccggcag gcgcgcgagg taggcgagcg aggcgcacag cgccagcgcg
    13141 ccgccgatgg cgagcgccgc cggcgggccg acatacagcg ccgcccagcc ggcggcgagc
    13201 gcgccgagcg ggctcatgcc gaggaatgac atgatgtaga gcgcggccac gcgcgcgcgc
    13261 aggcggtcct cgacgatcgt ctgcaggatc gtgttgatcg agcccgcggt gagcatcatg
    13321 cccatgccgg ccgcgtaggt cagcgcgagc gccggccaga accacgggca ccaggcgaac
    13381 gcgatcaccg cgatgccggc ggcggcgccg gcccagccga tcaccgtgag caggccgcgc
    13441 accgtcgggc gcaaggcgag cagcagcatg cccgacaccg cccccaggcc gccggcgccg
    13501 atcatccagc cgagcgtctg cgaattgccg ccgaacacgt cgcgcgcgaa aaacggcgcc
    13561 agcgcctgca gaggctgggt gccgacgctc accgccgcca gcagcagcag aatgctgcgc
    13621 gagggcagaa agccgtaggc ccagcgaaag cccgccgcga gctgcgccaa ccagccctgg
    13681 tgcgcgctct cgggcgggcg cgcctgcacc tgcaccgcgg cgagcgccgc gaggatcgcc
    13741 acgtacgagg cactgttcag cccgaagccc cacacttcgc cgcccagcgc gatcaccgcg
    13801 ccgccgacca tcggcccgac gaagcgcgcg ccgttcatca gcatcgactg gaaggcgatc
    13861 gcgttgggca ggtcttcgcg ccccgccacc agttcgatga ggaaggactg gcgcgccgga
    13921 ctgtcgaagg cgttgacgat gccgagcacc aggttgccgg cgatcaggtg ccagggctgg
    13981 atctggccgg aggccaccag cgccagcatg gccaaggatt gcgcgagcgc gaccgaattg
    14041 gtggcgcgca gcaccgtggt cttgctcaac cggtccaccc agacgcccgc catgggagcc
    14101 agcacg
//
LOCUS       DASRIE010000093         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2180903, whole genome shotgun sequence.
ACCESSION   DASRIE010000093 DASRIE010000000
VERSION     DASRIE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180903"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFB53_04265"
     CDS             complement(<1..257)
                     /locus_tag="VFB53_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN85983.1"
                     /translation="MSKAPAPPRPSGDLLKLDHQLCFALYSASLAMTRLYKPLLQPLG
                     LTYPQYLAMLALWERDAIPVSALGQRLQLDTGTLTPLLKRL"
     gene            369..2027
                     /locus_tag="VFB53_04270"
     CDS             369..2027
                     /locus_tag="VFB53_04270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HZN85984.1"
                     /translation="MTAPAIPANVRRSLRETFGITRLHDGQHHVIARVLEGGDTLAIM
                     PTGAGKSLCYQLPALHLEGTTVVVSPLISLMKDQADKLEAAGVAAEQLNSAQPKREQE
                     ASLERIAERDDTIVFATPERLGDPAFIATLQKRRIALFVVDEAHCISQWGHDFRPAFL
                     EIGHALRLLGNPPVLALTATAIADVIEDIRRQLDRPHMHVVNTGIYRPNLRFQVAQAT
                     REEAKFDRLREALGKARGCGIVYTATVKCCDEIHERLRAAGESVACYHGRMPTAERNA
                     SQDRFMRGEARVMVATNAFGMGIDKRDVRFVVHYQMPGNLESYYQEAGRAGRDGMPAD
                     CVLLFYRKDRQVQQFFLAKRYPSAEDVQAVQAVLTAAREPLSAEDIAATLKGVPANRI
                     SVALQLLRDAHVATANRARAWSLRPGAAADTPRIERMAQAYEEKAVRDQEALERMVFY
                     AQTGFCRWRVLLDYFDEPMDGERCGHCDNCLHPPVADELPEVDQITVAAAALHHVVPF
                     KAGDEVRVPRYGGGRVEQVAGDEVKVVFPDGAKRSFVAEFVQAA"
BASE COUNT          316 a          725 c          738 g          273 t
ORIGIN      
        1 tccaggcgct tgagcagcgg cgtgagcgtg ccggtgtcga gctgcaggcg ctgccccagg
       61 gccgacaccg ggatggcgtc gcgctcccag agcgccagca tcgcgaggta ctgcgggtac
      121 gtgaggccca gcggctgcag cagcggcttg tacagccgcg tcatggcgag cgaggccgaa
      181 tagagcgcga agcacagctg gtggtcgagc ttcagcaggt cgccggaagg gcggggcggg
      241 gcgggcgctt tcgacatggc ggcggattct attgcacgcg attgaaacgc gtaaaacctt
      301 ttcggccgct cgggggaccg ggtggcaccc cccggccccc cgggaacgtg ccttgccacc
      361 acggcttcat gaccgctccc gccatccccg ccaacgtgcg gcgctccctg cgcgagacct
      421 tcggcatcac gcggctgcac gacggccagc accacgtcat cgcgcgcgtg ctggaaggcg
      481 gcgacaccct ggccatcatg cccaccgggg ccggcaagtc gctgtgctat cagcttcccg
      541 cgctgcacct ggaaggcacg accgtcgtcg tctcgcccct catctcgctg atgaaggacc
      601 aggccgacaa gctggaggcc gccggcgtcg ccgccgagca gctcaacagc gcgcagccga
      661 agcgcgagca ggaggcttcg ctcgagcgca tcgccgagcg cgacgacacc atcgtgttcg
      721 ccacgccgga gcgcctcggc gacccggcgt tcatcgccac gctgcagaag cgccgcatcg
      781 ccctgttcgt cgtcgacgag gcgcactgca tctcgcaatg ggggcacgac ttccggcccg
      841 cgttcctgga gatcggccat gcgctgcggc tgctggggaa cccgccggtg ctcgcgctga
      901 ccgccaccgc gatcgccgac gtcatcgagg acatccggcg ccagctcgac cggccgcaca
      961 tgcacgtcgt caacaccggc atctaccggc cgaacctgcg cttccaggtg gcgcaggcga
     1021 cgcgcgagga agcgaagttc gaccgcctgc gcgaggcgct gggcaaggcc cggggctgcg
     1081 gcatcgtcta caccgccacg gtgaagtgct gcgacgagat ccacgagcgg ctgcgcgccg
     1141 ccggcgagtc ggtggcgtgc taccacggcc gcatgcccac ggcggagcgc aacgccagcc
     1201 aggaccgctt catgcgcggc gaggcccgcg tcatggtggc gaccaacgcg ttcggcatgg
     1261 gcatcgacaa gcgcgacgtg cgcttcgtcg tgcactacca gatgccgggc aatctcgagt
     1321 cgtactacca ggaggccggc cgcgccggac gcgacggcat gcccgccgac tgcgtgctgc
     1381 tcttctaccg caaggaccgg caggtccagc agttcttcct ggccaagcgc tacccgagcg
     1441 ccgaggacgt gcaggccgtc caggccgtgc tcaccgcggc gcgcgagccg ctgtcggcgg
     1501 aggacatcgc cgccacgctg aagggcgtgc cggccaaccg gatcagcgtc gcgctgcaac
     1561 tgctgcgcga cgcgcacgtc gcgacggcca accgtgcgcg cgcgtggtcg ctcaggccgg
     1621 gcgccgccgc cgacacgccc cgcatcgagc gcatggcgca ggcctacgag gaaaaggcgg
     1681 tgcgcgacca ggaggcgctg gagcgcatgg tgttctacgc gcagacgggc ttctgccgct
     1741 ggcgcgtgct gctcgactac ttcgacgagc cgatggacgg cgagcgctgc ggccactgcg
     1801 acaactgcct gcatccgccg gtcgccgacg agttgccgga ggtcgaccag atcaccgtgg
     1861 cggccgccgc cttgcaccac gtcgtgccgt tcaaggcggg cgacgaggtg cgcgtgccgc
     1921 ggtacggcgg cgggcgggtg gagcaggtcg ccggggacga ggtgaaggtg gtgtttcccg
     1981 acggggcgaa gcggtcgttc gtggcggagt tcgtgcaagc ggcgtgagcc gccgggggcc
     2041 gcagcctcag gc
//
LOCUS       DASRIE010000094         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3492910, whole genome shotgun sequence.
ACCESSION   DASRIE010000094 DASRIE010000000
VERSION     DASRIE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3492910"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="ccmI"
                     /locus_tag="VFB53_04275"
     CDS             <1..739
                     /gene="ccmI"
                     /locus_tag="VFB53_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HZN85985.1"
                     /translation="VSRRAANLAIHRDQLRELDADRAAGKLAQSDYDRSRAELEARVL
                     EDADAGEAAAPARAGGRGAAVAVGLALPLCALAVYFAVGNPRALDPGADPGAIGPAQI
                     EAMVERLAARLRENPDNADGWKMLGRSYAVLGRFDQAVDAYAKAALRLPRDAQLLADF
                     ADALGMARGQSLRGEPEQLLLRALEIDPQNLKALALAGTAAFDRRDFKAAAGYWERML
                     PLVPPGSEEAGVIQENVAEAHKRAAAR"
     gene            complement(741..1472)
                     /locus_tag="VFB53_04280"
     CDS             complement(741..1472)
                     /locus_tag="VFB53_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85986.1"
                     /translation="MLGDVPYSQAEAEKLDAVIGRMNAQELAFVVHVGDVGSSARTQA
                     CSEDWLEARQRQFAKIRHAFVLLPGDNEWSECADPAQRLAAWRRRFCAAVPGLALERQ
                     PGHCENLRWRAGGMAFIGLNVPGGGNPALADARMAATLEWLDESLSVAEEQRAAHIFV
                     FIHADPRFERVGATDSYARLRAALATHAAWFKGRLVLVHGDTHVYRDDEPVPGLRRIE
                     TWGSPFVSWLRAAVAGGALRVATGW"
     gene            complement(1588..>2688)
                     /locus_tag="VFB53_04285"
     CDS             complement(1588..>2688)
                     /locus_tag="VFB53_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZN85987.1"
                     /translation="VVDQCYLCDMCYMTKCPYVPPHPWNVDFPHLMLRAKAVKFRRHG
                     AGLRDRLLSSTDAMGKLMAIPVVAQAVNAANKLPLARGLMEKTLGVHRDRQLPDYASA
                     KFRVNAAAGKDHAVKDGKTTPGKAAIFATCYVNYNEPGMGHDLVALLEHNEVPCVLVE
                     KEACCGMPKLELGDLEAVARLKEKNLPLLAKLAREGYAILAPVPSCTLMFKQELPLMF
                     PQDAEVKAVQEAMFDPFEYFVQRAKDGLLKKDFKQALGKVSYHIPCHSRVQNMGQKTR
                     EALEMVPGTQVTTVERCAGHDGTWGVKREFFDNSMKIGGPVFRQMAAPAPDYVSSDCP
                     IAARHILQGMGADAGQAEKAHPLTLLRKAYGI"
BASE COUNT          382 a          942 c          962 g          402 t
ORIGIN      
        1 ggtgtcgcgg cgcgccgcca acctcgccat ccatcgcgac cagctgcgcg agctcgacgc
       61 cgaccgtgcc gcgggcaagc tcgcgcagtc ggactacgac cggtcgcgcg cggagctgga
      121 ggcgcgggtg ctcgaagacg cggatgcggg cgaggccgcg gcgccggcgc gcgccggcgg
      181 tcgcggcgcg gcggtcgcgg tgggcctcgc cctgccgctg tgcgcgctgg cggtctactt
      241 cgccgtcggc aatccgcgcg cgctcgatcc gggcgcagat cccggcgcca tcggccccgc
      301 gcagatcgag gcgatggtgg agcgtctcgc ggcgcgcctg cgcgagaacc ccgacaacgc
      361 cgacgggtgg aagatgctcg ggcgctccta cgcggtgctg gggcgcttcg accaggcggt
      421 ggacgcctac gccaaggcgg cgctgcgcct gccgcgcgac gcgcagctgc tcgccgattt
      481 cgccgacgcg ctcggcatgg cgcgcggcca gagcctgcgc ggcgagcccg agcagttgct
      541 gctgcgtgcg ctggagatcg acccgcagaa cctcaaggcg ctggcgctcg ctggtaccgc
      601 ggccttcgac cgccgggact tcaaggcggc ggccggctac tgggagcgca tgctgccgct
      661 ggttccgccg ggctcggagg aggcgggcgt catccaggag aacgtcgcgg aggcgcacaa
      721 gcgcgcggcg gcgcgctgag ctaccagccg gtcgcgacgc gcagcgcgcc gcccgccacc
      781 gcggcgcgca gccaggagac gaagggcgaa ccccaggtct cgatgcgccg cagtcccggg
      841 acgggctcgt cgtcgcgata cacgtgcgtg tcgccgtgca ccaggaccag gcggcccttg
      901 aaccacgccg catgggtggc gagcgcggcg cgcaagcgcg cgtagctgtc cgtggcgccc
      961 acgcgctcga agcgcgggtc ggcgtgaatg aaaacgaaga tgtgcgcggc gcgctgctct
     1021 tcggcgacgg acaggctctc gtcgagccat tccagcgtcg ccgccatgcg cgcgtcggcg
     1081 agcgccggat tgccgccgcc gggcacgttc aggccgatga acgccatgcc gcccgcgcgc
     1141 cagcgcaggt tctcgcagtg ccccggctgg cgctcgagcg cgaggcccgg aaccgcggcg
     1201 cagaagcgcc ggcgccaggc ggccaggcgc tgcgcggggt cggcgcattc gctccactcg
     1261 ttgtcgccgg gcagcaggac gaaggcgtgg cggattttcg cgaactggcg ctggcgcgcc
     1321 tccagccagt cctcgctgca ggcctgcgtg cgcgcgctcg agcccacgtc gccgacgtgc
     1381 acgacgaacg cgagttcctg tgcgttcatg cggccgatca ccgcgtcgag cttctccgcc
     1441 tcggcctgcg agtagggaac gtcgccgagc acgccgaagg cgaactcccc gccgggcagc
     1501 ggcgccagct gcgcgcagcc cgcaacaaca aaaaataggg tcagacccca ttttcttgaa
     1561 aaatggggtc tgaccctatt tttgacgtca gatgccgtag gctttgcgca gcagggtgag
     1621 ggggtgggcc ttctcggcct ggccggcgtc ggcgcccatg ccctgcagga tatggcgggc
     1681 ggcgatcggg cagtcggaac tgacgtagtc gggcgccggc gcggccatct ggcggaacac
     1741 cgggccgccg atcttcatcg agttgtcgaa gaattcgcgc ttcacgcccc aggtgccgtc
     1801 gtggccggcg cagcgctcca ccgtggtcac ctgcgtcccc ggcaccatct cgagggcctc
     1861 gcgcgtcttc tggcccatgt tctgcacgcg cgagtggcag gggatgtggt acgaaacctt
     1921 gccgagcgcc tgcttgaagt cctttttcag caggccgtcc ttcgcgcgct gcacgaagta
     1981 ctcgaagggg tcgaacatcg cctcctgcac cgccttgacc tccgcatcct gcgggaacat
     2041 gagcggcagc tcctgcttga acatcagcgt gcaggagggc acgggggcga ggatcgcgta
     2101 accctcgcgc gccagcttcg ccaggagcgg gaggttcttt tccttcagcc gcgccaccgc
     2161 ctcgaggtcg ccgagctcca gcttcggcat gccgcagcag gcttccttct cgacgagcac
     2221 gcagggcact tcgttgtgct cgagcagcgc gacgaggtcg tgccccatgc ccggctcgtt
     2281 gtagttcacg tagcaggtgg cgaagatcgc cgccttgccc ggggtggtct tgccgtcctt
     2341 caccgcgtga tccttgcctg ccgccgcatt gacgcggaac ttcgcgctgg catagtcggg
     2401 aagctgccgg tcccggtgca cgccgagcgt cttttccatc aggccgcgcg cgagcggcag
     2461 cttgttcgcc gcgttcaccg cctgcgccac cacggggatc gccatcagct tgcccatcgc
     2521 atcggtggag gaaaggagtc gatcgcgcag gcccgcgcca tgccggcgga acttcaccgc
     2581 cttggcgcgc agcatcaggt gcgggaaatc cacgttccag gggtgcggcg gcacgtaggg
     2641 gcacttggtc atgtagcaca tgtcgcagag gtagcactgg tccaccac
//
LOCUS       DASRIE010000095         7352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_637424, whole genome shotgun sequence.
ACCESSION   DASRIE010000095 DASRIE010000000
VERSION     DASRIE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637424"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1132..2256)
                     /locus_tag="VFB53_04290"
     CDS             complement(1132..2256)
                     /locus_tag="VFB53_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN85988.1"
                     /translation="MTHYFPSHGGGVEAVAGELASRLVASGKAEIAWFSSDTDAPPPD
                     SAELRHIPISACNIAERRFGFPYPLWSPSGLAKLVRGVRQADAVHLHDCLYLPCIVAW
                     AAARASRRPVIVTQHVGAIPYRNPLLRAALAVANRVFGKLVLGTSTQAVFVSETVRRY
                     FGGFVRWPSPPPVVANGVDTERFAPVEAARREALRAALGVGRNQPLVLFVGRFVEKKG
                     LHLLRQLAQRLPHARWIFAGWGPLDPADWGLANVSVPGKLTREALVPLYQAADLLVLP
                     SVGEGFPLVVQEAMACGTPALVSDETAAASPAAAGLLLHEPLPGDDDAERWSARLRAL
                     LAAPEALEALRPRVAAFARRHWSWTSCVEQYAAIFAACAR"
     gene            2326..4356
                     /locus_tag="VFB53_04295"
     CDS             2326..4356
                     /locus_tag="VFB53_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85989.1"
                     /translation="MQNVFSAAAALLGRRSLETTLVLAPPLAAIFWAGGTVSLAAAAA
                     AMLVVVYVVVSAGCLILRSANAADMPAPAAWVLGVVASSVATYLLVVCFQVLAATAFA
                     IWGAIVIAFTFVRRAQRAGQAGLTRSELAALLLCALATLGWCWEIAGASHSYIQGGIF
                     TTWTDQFAHAGAISTFGDPRAAGHQAIELAGMPRPPYHYASYMLPAALAWPLDLAGLS
                     LATSVWVPIGFLSICAGAYTLGAVLGGRAAGFLALAALTLLPDAASYGLYNRLFGFFW
                     YVLAGPSASYAVGLSLVAIALLHRWHQTRDWRPLLVGACMVAALAVVRVHILLLMLPA
                     WLSCAILCTRLSRERKLMSLAGVLALFLLFVLGFYRYFPDTPTALGKFLEVAHQDQDP
                     TAYTGLYGDLMKRYGPLIAFPVGLLLVFPACLGIFLLLYPLAMLAMRRYRGLQATDWM
                     PLVMCAWYLLLMTTAPMPPHGDSTEWTQRPFVVLYAVFVVWTAAGLSTWLASLGWSAR
                     RLAWTFAVAVPLLGAAILNSTVNDFRWTIQIWAPQGLPAAAAFIRAHWRPGDVLAVHR
                     LRKVLVVTDTAVEAVALTGMPAYIARPYIQFARAGRPGEVARERFGQLIEIGVEPTAA
                     SALARLRQLGIQWYIISDDEGPGWDPERRQAAFTQGVVAVYSTDPARFTARR"
     gene            complement(4340..5197)
                     /locus_tag="VFB53_04300"
     CDS             complement(4340..5197)
                     /locus_tag="VFB53_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZN85990.1"
                     /translation="MSIAEFVYTVLLKPPPLRRAANAAIRGLLPERVRVGGAEVWLNP
                     DDPVISGALRFGVYERGEIAFFRSRFRPGMTLVDVGANVGLYSALALSTPGFHGRVLA
                     LEPHAESRRFLEKTIAGNAPAAGAAQIVGLAAADRPGTLALYKNPENKGDNRLYPDPL
                     LRGEESVGADTLDNICRRAGIASAQFVKIDVQGAEAQVVRGAAGLLGASAECILMTEF
                     WPYGLARCGGDALDYLGMLRDLGFRLYQLSGKRGTLTPIGSPEALLGRTQGRRYANLV
                     GLKGASAAP"
     gene            complement(5194..6168)
                     /gene="galE"
                     /locus_tag="VFB53_04305"
     CDS             complement(5194..6168)
                     /gene="galE"
                     /locus_tag="VFB53_04305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZN85991.1"
                     /translation="MKILVTGGAGYIGSHMVKLLAETGSEVTVLDDLSTGHAEAVRGA
                     QLVKGDIVDVAATRSLLQKNRIEAVVHFAASSLVGESMADPLKYYRRNVGGTAALVQA
                     MREAGVAHIVFSSTAAVYGDPVRTPIDEAHPKQPVNPYGASKLAVERMLSDCSTAYGI
                     GAVVLRYFNAAGADPSGELGERHDPETHLIPLVLQAASGRRASISVFGNDWPTRDGTC
                     VRDYIHVNDLCDAHLRALDWLAKGGRYEAFNLGNGDGATVLEVIEAARRVTGKPIKID
                     QAARRAGDPATLVADATRARRVLGWHPARADIEGIVRDAWNFEQRRPR"
     gene            6237..6980
                     /gene="tfpZ"
                     /locus_tag="VFB53_04310"
     CDS             6237..6980
                     /gene="tfpZ"
                     /locus_tag="VFB53_04310"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HZN85992.1"
                     /translation="MSRWRAAGIHLLISAGIAAGVLALMLGVWYGPTLFRAMGGTWLA
                     LILIGVDVVLGPALTLVVFRSGKRGLKFDLVAIALLQIAALAYGCYVVSLARPAFIVF
                     VKDQFQVATVAELDPEQLAKARYPQYRSPPWSGPELVFGEWPKDHEEQQQLMFAGLAG
                     VDLQHFPKYYAPYEQGRDQILARAQPLSSVGRLEPAAAKAIDAWLASAGADPANLRYL
                     RLRGRIAWVAVLIDARSAAPVKMLLAEKL"
     gene            complement(7033..>7352)
                     /locus_tag="VFB53_04315"
     CDS             complement(7033..>7352)
                     /locus_tag="VFB53_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN85993.1"
                     /translation="IMLVSVTERTREIGLRMAVGARTRDILGQFLVEAVTLSLIGGLI
                     GVALGMGTALAIAEFAGWRISISPQAIALAVAFAFVIGVFFGFYPARKAARLNPVEAL
                     RFE"
BASE COUNT         1094 a         2552 c         2595 g         1111 t
ORIGIN      
        1 gcggaagtcc cgggaccgcc cagctccaca gccgcacctg ctcggacagg cgcctgagga
       61 ccgtctcttc accgagaaag gagaacgcgg ttcctagctc gagcagcagg tacgcgatca
      121 ggtttcccgc gcgatgcaga ggattgccgt cgggcgggta gggcacgacg aagagcgggc
      181 cctgcagctc gcgcaggaag agccaccagc cgagcccgag cacgatcgcg agcgggacat
      241 agcccgcggc cagcgcgagc agatcacgcc gcgaccccgg gcgacgggcg atccagagaa
      301 tccacggcag cgcgaacagc gcgtgcggga ccgggttgct cagcacgagc gcgaaggagc
      361 cgaccacgcc cgccgcggcc agcgacctcg tgcttccttt cagcaccagc cacgcaaaca
      421 gcaggttcag gcaaaggtgt gcgctcatcg aaaagtacga cagcgccatg ccggtgaacg
      481 ccggcgagcc gagggcgaag agcatcgccc acccgccggc ttgcgcattg ccggtcagcc
      541 gcgcggcgag tttcgccatc agcaccagcg agagggaagc gagcaacggg ttgcaggccc
      601 agggagcgcc gaccaggctg aacggcgcga gcagcagggc gaatcccggc cagtagacgg
      661 cggcgacggc gccggtcgcg tccgaggcga tcagccatct gccctggtaa tggggcggga
      721 tcagcagcgc gagcaggtcg cgcgggaact ggcccgccag gtgcccggcc gcgaacgccc
      781 ggctctggaa caccgccagg tgctcgtccc ccgcgagcgg atggttgtgc gccaccacca
      841 gcaccgccgt gcagagcacg acgaagctcg cgcccgcaac ccgccaggga tggcgcgaca
      901 gcgcatcgat cagatcgagc gccgcgctgc gggtctgcgg caggcaggcg ccgacggcga
      961 tcgcgatgat cagcgcgctg ccggcgagat cctgctcgcg cagcaggtac tggaaaatca
     1021 gcagccgcca ttgcggatcc ggatacttgg tgtagtcgag gatgaagatg atcgacagca
     1081 atgccgccag aaccgtgagc gcgcagagcg ccgatgtcca tcggggcgtc tctagcgtgc
     1141 gcacgcggcg aagatggcgg cgtattgctc cacgcagctg gtccacgacc agtggcggcg
     1201 ggcgaacgcg gccacgcggg gtctgagcgc ctcgagcgct tccggcgcgg cgagcagcgc
     1261 ccgcagccgc gccgaccacc gctccgcgtc gtcatcgcca ggcagcggct cgtgcagcag
     1321 caggcctgcg gcagccgggc tcgcggcggc cgtctcgtcg ctcaccagcg ccggggttcc
     1381 gcaggccatc gcttcctgca ccaccagcgg aaagccttcg cccacgctcg gcagcacgag
     1441 aaggtcggcg gcctggtaga gcggaaccag cgcttcgcgc gtgagcttgc cggggaccga
     1501 cacgttggcg aggccccagt ccgcggggtc gagcggtccc cagccggcga agatccagcg
     1561 cgcatgcggc aggcgctgcg ccagctgccg cagcagatgc agtcccttct tttcgacgaa
     1621 ccggccgacg aacagcacga gcggctgatt tctcccgacg ccgagcgctg cgcgcagcgc
     1681 ttcgcggcgc gccgcttcca ccggcgcgaa gcgctcggtg tccacgccgt tggccaccac
     1741 cggcggcggg ctgggccagc gcacgaagcc gccgaagtac cggcgcacgg tctcgctgac
     1801 gaacaccgcc tgcgtcgatg tccccagcac cagcttgccg aacacccggt tcgccacggc
     1861 gagcgcggcg cgcaacaacg ggttgcgata cggaatggcg ccgacgtgct gggtgacgat
     1921 cacgggtcgc cgcgaagcgc gcgcagcggc ccacgcaacg atgcagggca ggtacaggca
     1981 atcatgcaga tggacggcgt ccgcctggcg tacgccgcgc accagcttcg ccagcccgga
     2041 aggactccac agcggatagg gaaagccgaa tcggcgctcg gctatgttgc aggcgctgat
     2101 cgggatgtga cgcagttccg ccgagtcggg cggcggcgca tcggtgtcgc tcgagaacca
     2161 ggcgatctcg gctttgccgc tcgcgacgag acgcgaggcg agctcgcccg cgactgcttc
     2221 gacgccgccc ccatggctcg ggaagtagtg cgtcacgacg acaagccgtg gattcgacac
     2281 tgtagtcttt ttcggcaagt ataagaatta tcgccgctcg agaccatgca aaacgttttt
     2341 agcgcagccg ccgcgctgct cggccgccgg tcgctcgaga ccacgctggt tctcgcgccg
     2401 ccgctcgcgg cgatcttctg ggccggcgga acggtatcgc tcgccgcggc ggccgcggcg
     2461 atgctcgtcg tggtctatgt cgtggtcagc gccggctgcc tgatcctgcg cagcgccaat
     2521 gccgccgaca tgccggcgcc cgcggcctgg gtgctcggcg tggttgccag ctcggtcgcg
     2581 acctatctgc tggtcgtctg cttccaggtt ctcgccgcca cggcgttcgc gatctggggc
     2641 gcgatcgtga tcgccttcac cttcgtccgg cgcgcgcagc gcgcagggca ggccggcttg
     2701 acccgcagcg agctggccgc attgctgctc tgcgcgctcg ccaccctggg atggtgctgg
     2761 gagatcgcgg gggcatcgca ctcgtacatc cagggcggca tcttcaccac ctggaccgat
     2821 cagttcgccc acgccggcgc gatttcgacg ttcggcgatc cgcgcgccgc gggtcaccag
     2881 gcgatcgagc tcgccggcat gccgcggccg ccctatcact acgcttccta catgctgcct
     2941 gcggcgctcg cgtggccgct cgacctggcg ggcctctcgc tcgcgacttc ggtctgggtg
     3001 ccgatcggct ttctcagcat ttgcgccggc gcctatacgc tcggcgcggt cctcggcggg
     3061 cgggcggccg gtttcctcgc gctcgctgcc ctgaccctgt tgccggacgc ggcgagctac
     3121 ggcctgtaca accgcctgtt cgggttcttc tggtatgtgc tggcgggtcc cagcgcctcg
     3181 tacgcggtgg gtttgtcgct cgtcgccatc gcgttgctcc atcgctggca ccagacgcgc
     3241 gactggcgtc cgctgctggt cggcgcgtgc atggttgccg ccctggccgt ggtgcgcgtc
     3301 cacatcctgc tgctcatgct gccggcgtgg ctgagctgcg cgatcttgtg cacgcggctc
     3361 agccgcgagc gcaagctgat gtcgctggcg ggcgtgctcg ctctcttcct gctgttcgtg
     3421 ctgggcttct accggtactt cccggacacg ccgaccgcgc tgggcaagtt tctggaggtg
     3481 gcgcaccagg atcaggatcc caccgcgtac acggggctgt acggcgatct gatgaagcgc
     3541 tacgggccgc tgatcgcgtt cccggtcggc ctgctgctcg tgttccccgc ctgtctcggc
     3601 atcttcctgc tcctctatcc gctggccatg ctcgcgatgc ggcgctaccg cggcctgcag
     3661 gcgacggatt ggatgccgct ggtcatgtgc gcctggtacc tgctgctcat gacgacggcg
     3721 ccaatgccgc cgcatggcga ttccacggaa tggacccagc ggccgttcgt cgtgctttat
     3781 gccgtattcg tggtctggac cgcggcaggc ctgagcacgt ggctggcgtc gctgggatgg
     3841 agcgcacgcc gcctggcatg gacgttcgcc gtggcggtgc cgctgctcgg cgcggcgatc
     3901 ctgaactcca cggtcaacga cttccgatgg acgatccaga tctgggcgcc gcaggggctg
     3961 cccgcggccg ccgcgttcat tcgcgcgcac tggcggcccg gcgacgtgct ggcggtgcat
     4021 cgcctgcgca aagtgctggt cgtgaccgat accgcagtgg aggcggtcgc gctcaccggc
     4081 atgccggcct atatcgctcg cccgtacatc cagttcgcca gagccgggcg cccgggcgag
     4141 gttgcccggg agcgcttcgg tcagctgatc gagatcgggg tagagccgac cgccgcgtca
     4201 gcgctcgcgc ggttgcggca gctcggcatc cagtggtaca tcatttccga cgacgagggc
     4261 cccggctggg atcccgagcg ccggcaggcg gcgttcacgc aaggcgtcgt ggccgtctat
     4321 tcgaccgacc cggcaaggtt cacggcgcgg cgctagcgcc tttcaagccg accaggttgg
     4381 cgtagcgcct gccttgcgtg cggccgagga gcgcctcggg tgagccgatc ggcgtcagcg
     4441 tgccgcgctt tccggatagc tggtacagac ggaaccccag gtcgcgcagc atcccaaggt
     4501 agtcgagcgc atcgccgccg caacgcgcaa ggccataggg ccagaactcc gtcatcagga
     4561 tgcattcggc actcgcgccg agcaggccgg ccgcgccgcg caccacctgc gcttcagcgc
     4621 cctgcacgtc gattttcacg aactgcgccg acgcgatgcc cgcccggcgg cagatgttgt
     4681 cgagcgtatc ggcgcccacg ctctcttctc cccgcagcag cgggtcggga taaaggcggt
     4741 tgtcgccctt gttctccgga ttcttgtaga gcgcgagcgt gcccgggcgg tccgcggcgg
     4801 cgagaccgac gatctgcgcc gcgcccgcgg caggcgcgtt gcccgcgatg gtcttctcca
     4861 ggaagcgccg gctttcggcg tgcggctcga gcgcgagcac ccggccgtgg aatcccggcg
     4921 tcgacagcgc gagggcgctg tacaggccga cattcgcacc gacgtcgacc agggtcatgc
     4981 cagggcggaa gcgcgagcgg aagaaggcga tctcgccgcg ctcgtagacg ccgaatctca
     5041 gggcgccgga aatcaccgga tcgtccggat tgagccagac ctcggcgccg ccgacgcgca
     5101 cgcgctcggg gagcaggccg cggatcgccg cgtttgccgc gcggcgcagc ggcggcggct
     5161 tgagcagcac cgtatagacg aactcggcga tgctcaccgc ggccggcgct gctcgaaatt
     5221 ccaggcgtcg cgcacgatgc cttcgatgtc ggcgcgcgcc gggtgccagc cgaggacgcg
     5281 ccgcgccctg gtggcgtcgg cgaccagcgt ggcgggatcg cccgcgcggc gcgcggcctg
     5341 gtcgatcttg atcggcttgc cggtcacgcg gcgcgcggcc tcgatcacct cgagcacggt
     5401 ggcgccgtcg ccgttgccga gattgaacgc ctcgtagcgc ccgcccttgg cgagccagtc
     5461 gagcgcgcgc aggtgcgcgt cgcacaggtc gttgacgtgg atgtagtcgc gcacgcaggt
     5521 gccgtcgcgc gtcggccagt cgttgccgaa caccgagatc gaggcgcgcc gccccgaagc
     5581 ggcctgcagc acgagcggaa tcagatgggt ctcgggatcg tggcgctcgc cgagctcgcc
     5641 cgaggggtcg gcgccggccg cgttgaagta gcgaagcacg acggcgccga tgccgtaggc
     5701 ggtgctgcag tccgagagca tgcgctcgac ggcgagcttg ctcgcgccgt agggattgac
     5761 cggctgcttg ggatgcgcct cgtcgatcgg cgtgcgcacc gggtcgccat acaccgcggc
     5821 cgtggacgag aacacgatgt gcgcgacgcc cgcctcacgc atggcctgca ccagcgcggc
     5881 ggtgccgccg acgttgcgcc ggtagtactt cagcgggtcg gccatcgact cgccgaccag
     5941 gctcgacgcc gcgaagtgga ccacggcttc gatgcggttt ttctgcagca ggctccgggt
     6001 ggccgcgacg tcgacgatgt cgcccttgac gagctgcgcg ccacgcaccg cctcggcgtg
     6061 cccggtggaa aggtcgtcga gcaccgtgac ctcgctgccg gtctcggcaa gcagcttcac
     6121 catgtgcgag ccgatgtagc cggcgccgcc cgtcaccagg atcttcattg gagcggcgga
     6181 ttgtagctaa gatacgcacc cgagcgcgta tggagaaaag cgacggaaga ccagtcatga
     6241 gccggtggcg ggccgcgggc atccacctgc tgatcagcgc cggcatcgcc gccggcgtgc
     6301 tcgccctgat gctgggcgtg tggtacgggc cgacgctgtt ccgggccatg ggcggcacgt
     6361 ggctcgcgct gatcctgatc ggcgtcgacg tcgtgctcgg acccgcgctc accctggtcg
     6421 tgttccgcag cggcaagcgc ggcctgaagt tcgatctcgt ggcgattgcg ctgctccaga
     6481 tcgccgcgct cgcctacggc tgctacgtcg tctcgctcgc gcgacccgcg ttcatcgtct
     6541 tcgtcaagga ccagttccag gttgccaccg tcgccgagct cgatccggag cagctcgcca
     6601 aggcgagata ccctcagtat cgcagcccgc cgtggtcggg acccgagctg gtgttcggcg
     6661 agtggccgaa ggaccacgag gagcagcagc agctcatgtt cgccgggctc gcgggcgtgg
     6721 acctgcagca ctttcccaag tactacgcgc cctacgagca gggccgggac cagatcctgg
     6781 cgcgggcgca gccgctgtcg agcgttggcc gcctggagcc ggcggccgcg aaggcgatcg
     6841 acgcgtggct cgcgagcgcc ggcgccgatc cggcgaacct gcgctatctg cggctgcgcg
     6901 ggcgcatcgc ctgggtggcg gtgctgatcg acgcgcgcag cgccgcgccg gtgaagatgc
     6961 tgctcgcgga gaagctctag ggatccccgc gaaggaacgt cacccccgaa tggttcagtc
     7021 gggggcgggg gctcactcga agcgcagcgc ctcgaccgga ttgaggcgcg ccgccttgcg
     7081 cgccgggtag aagccgaaga acacgccgat cacgaaggca aacgccaccg cgagggcgat
     7141 ggcctggggc gagatcgaga tgcgccagcc ggcgaactcg gcgatcgcga gcgcggtgcc
     7201 catgccgagg gcgacgccga tcagcccgcc gatcagcgac agcgtcaccg cctcgaccag
     7261 gaactggccg aggatgtcgc gcgtgcgcgc gccgaccgcc atgcgcaggc cgatctcgcg
     7321 cgtgcgctcg gtgaccgaca ccagcatgat gt
//
LOCUS       DASRIE010000096         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_885312, whole genome shotgun sequence.
ACCESSION   DASRIE010000096 DASRIE010000000
VERSION     DASRIE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885312"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFB53_04320"
     CDS             <1..533
                     /locus_tag="VFB53_04320"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HZN85994.1"
                     /translation="LGAAGMDRATADYMGMLATVMNALALQDAMRRAGIDSRVQSALN
                     IEQVVEPYIRGKAIRYLEEGKVVVFAAGTGNPFFTTDTAAALRASEMGAEIVLKGTKV
                     DGVYTADPRKDAAAKRSQRLSFDEAIQRNLKVMDATALTLCRDQKLPVNVFSIFRKDA
                     LLRVVVGEDEGTLVHV"
     gene            547..1104
                     /gene="frr"
                     /locus_tag="VFB53_04325"
     CDS             547..1104
                     /gene="frr"
                     /locus_tag="VFB53_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZN85995.1"
                     /translation="MLAELRKNTEQKMHKSVEAFKGDLSKVRTGRAHTGLLDHITVDY
                     YGTPTPLAGVAKVILLDARTIGVSPFEKKMIPAVEKAIRESDLGLNPATAGDTIRVPM
                     PSLTEERRKELIKVVRHEAENARVAVRNLRRDANHHLKEALKKKEVSENDERRAQDEV
                     QKLTDRSIGEIDKLLQQKEAELLAV"
     gene            1112..1900
                     /gene="uppS"
                     /locus_tag="VFB53_04330"
     CDS             1112..1900
                     /gene="uppS"
                     /locus_tag="VFB53_04330"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZN85996.1"
                     /translation="MSQPSSTEAIPQHGAVPRHIAVIMDGNGRWAKRRHLPRFAGHRR
                     GVEAVRDAVQACAERGVRFLTLFAFSSENWRRPAEEVALLMQLFHGALSNEVEKLHRN
                     GIRLRVVGDLARFDSRMRALIEQGERLTAANARLTLTIAANYGGRWDILQAMHRLLRE
                     HPEAAQAPIEEAQLAPYLSMSHAPEPDLFIRTGGEQRVSNFLLWQLAYTELYFTETLW
                     PDFDRAALDAAIASYAQRERRFGRTSEQLEEAKPPASAKTGATA"
     gene            1887..2711
                     /locus_tag="VFB53_04335"
     CDS             1887..2711
                     /locus_tag="VFB53_04335"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN85997.1"
                     /translation="MPLPEGLWLRVATAAALLAGLLAALLLLEARQLALLLGVAVALA
                     GHEWGRLVGLERQGALGYAALCAALFGLAAAAGFAFPQLHPALFAASALFWLLLAPAW
                     LHRGVGAGARLTLSAAGIVVLVPTGMAVLTLPPDRLLLLLGLVWIADTAAYFAGRAFG
                     RRLLAPTISPGKTWEGVAGAVAATLIYAIICAVPGAPFAERVRDAAWIPYLGGAVLLC
                     ALSIVGDLFESALKRRAGVKDSGRLLPGHGGILDRIDSMTSSLPLAALLLAWLRVA"
     gene            2708..>3639
                     /gene="dxr"
                     /locus_tag="VFB53_04340"
     CDS             2708..>3639
                     /gene="dxr"
                     /locus_tag="VFB53_04340"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZN85998.1"
                     /translation="MTPQPATASARRPQRLVLLGATGSIGASTLDVIARHADRYAVQA
                     LSAHASAERLLELCVLHRPRRAVLSGCAEDRALRQRFADAGCELLFGAAALEEVAAAA
                     DCDTVMAAIVGAAGLPATLAAARAGKRLLLANKEALVMAGPLFMRAVRDSGATLLPID
                     SEHNAVFQCLPQEGGAVTLRGVRRIVLTASGGPFRSMPLERLHGVTPQQACAHPNWVM
                     GRKISVDSATMMNKGLEVIEACWLFGLPATAVEVVIHAQSIVHSLVEYVDGSLIAQLS
                     NPDMRVPIAHALGFPDRLESGARALDLGAVGQLS"
BASE COUNT          538 a         1263 c         1300 g          538 t
ORIGIN      
        1 cgctgggtgc cgcgggcatg gaccgcgcca ccgccgatta catgggcatg ctggccacgg
       61 tgatgaatgc gctcgccctg caggacgcca tgcgccgcgc cggcatcgac agccgcgtgc
      121 aatcggcgct caacatcgag caggtcgtcg agccctacat ccgcggcaag gcgatccgct
      181 acctggaaga gggcaaggtg gtggtgttcg ccgccggcac cggcaatcct ttcttcacca
      241 ccgacacggc ggcggcgctg cgcgccagcg aaatgggcgc ggagatcgtg ctcaagggca
      301 ccaaggtgga cggggtatat accgcggacc cgaggaagga cgccgccgcg aagcgctccc
      361 agcgcctgtc gttcgacgag gccatccagc gcaacctgaa ggtgatggat gccaccgcgc
      421 tgacgctgtg ccgcgaccag aagctgccgg tgaacgtgtt cagcattttc cgcaaggacg
      481 cgctgctgcg cgtcgtcgtc ggcgaggacg aaggcacgct ggtgcacgtc tgagggaggg
      541 agtccaatgc ttgccgaact gcgcaagaac accgaacaga agatgcacaa gagcgtggag
      601 gccttcaagg gcgacctgtc caaggtacgc accgggcgcg cccacaccgg gctgctggac
      661 cacatcacag tggactatta cggcaccccg acgccgctgg cgggggtggc caaggtgatc
      721 ctgctggatg cgcgcaccat cggcgtgtcg ccgttcgaga agaaaatgat cccggcggtg
      781 gaaaaggcga tccgcgaatc cgacctcggg ctcaacccgg ccaccgcggg cgacacgatc
      841 cgcgtaccga tgccctcgct caccgaggag cggcgcaagg agctgatcaa ggtggtgcgc
      901 cacgaggccg agaacgcgcg cgttgcggtg cgcaacctgc ggcgcgacgc caatcaccac
      961 ctgaaggagg cgctgaagaa gaaggaggtg tcggaaaacg acgagcgccg cgcccaggac
     1021 gaggtgcaga aactcaccga tcgctcgatc ggcgagatcg acaagctgct gcagcagaag
     1081 gaagccgagt tgctcgcggt ctaggcccag cttgtcccag cccagctcca ccgaagcgat
     1141 accgcagcac ggcgccgtgc cacggcacat cgcggtgatc atggacggca atggccgctg
     1201 ggcgaagcgc cggcacctgc cgcgcttcgc cggccaccgg cgcggcgtgg aggcggtgcg
     1261 cgacgcggtg caggcctgcg ccgaacgcgg tgtgcgcttc ctcaccctgt tcgccttcag
     1321 ctccgagaac tggcgccggc ccgccgagga ggtggcgctg ctgatgcagc tgttccacgg
     1381 tgcgctgagc aacgaagtgg agaagctgca tcgcaacggc atccggctgc gcgtggtcgg
     1441 cgacctggcg cgcttcgatt cgcgtatgcg cgcgctgatc gagcagggcg agcggctgac
     1501 cgccgcaaac gcgcggctca cgctgaccat cgccgcgaac tacggcggcc gctgggacat
     1561 cctgcaggcc atgcaccgcc tgctgcgcga gcatccggaa gcggcgcagg cgcccatcga
     1621 ggaagcgcag ctggcgccct acctgtcgat gagccacgcg cccgagccag acctgttcat
     1681 ccgcaccggg ggcgagcagc gcgtgagcaa tttcctgctg tggcagcttg cctataccga
     1741 gctttacttc accgagacgc tgtggcccga tttcgaccgc gccgcgctcg acgccgcgat
     1801 cgcgtcgtac gcgcagcgcg agcgccgctt cgggcgcacc agcgagcagc tcgaggaggc
     1861 gaagccccca gcatccgcga agaccggtgc cactgcctga aggcctgtgg ctgcgcgtag
     1921 ccacggccgc tgccttgctg gcggggctgc tcgcggcgct gctgctgctc gaagcgcgcc
     1981 agctggcgct gctgctgggc gtcgcagtgg cgctggcggg gcacgaatgg ggccggttgg
     2041 tcggcctcga gcgccagggc gcgctgggct atgcggcgct gtgcgccgcg ctgttcggcc
     2101 tggcggccgc cgcaggcttc gcgtttccgc agctccaccc ggcgctgttc gcggcgagcg
     2161 cgctgttctg gctgctcctt gcgcccgcct ggctgcaccg cggggtcggc gccggcgcgc
     2221 gcctgaccct gtcggcggcc ggcatcgtcg tgctggtgcc gaccggcatg gcggtgctga
     2281 cgctgccgcc ggatcgcctg ctgctgctcc tcggcctggt gtggatcgcg gacactgccg
     2341 cgtacttcgc cggccgcgca ttcggccggc gtctgctggc cccgacgatc agcccgggca
     2401 agacctggga gggcgtggcc ggcgccgtcg ccgccaccct tatctacgct atcatctgcg
     2461 cggtaccggg cgcgccattc gcggagcgcg tgcgcgatgc ggcctggatt ccctatctgg
     2521 gcggagccgt gctcctgtgc gcactcagta tcgtgggcga cctgttcgaa tcggcactca
     2581 agcgccgggc gggcgtcaag gacagcggca ggctgctgcc gggacacggc ggcatccttg
     2641 accgcatcga cagcatgacc tcgagcctgc cgctggccgc gctgctcctc gcctggctgc
     2701 gcgtcgcatg acaccgcagc ccgccacggc ctccgcgcgc cggccccagc ggctggtgct
     2761 gctcggcgcc actgggtcga tcggcgccag cacgctggac gtgatcgcgc ggcatgccga
     2821 ccgctacgca gtgcaggcgc tgagcgcgca cgcttccgcc gagcgcctcc tggaattgtg
     2881 cgtgctgcac cggccgcgcc gcgccgtgct ttccggctgc gccgaggacc gcgccttgcg
     2941 ccagcgtttt gccgacgccg gctgcgagct gctgttcggc gcggccgcgc tggaggaggt
     3001 ggccgccgcg gccgattgcg ataccgtgat ggccgccatc gtcggcgccg ccggactgcc
     3061 tgcgacgctg gcggcggccc gcgccgggaa gcggctgctg ctcgccaaca aggaggcgct
     3121 ggtgatggcg ggccccctgt tcatgcgcgc ggtgcgcgat tccggcgcga cgctgctgcc
     3181 gatcgacagc gagcacaacg ccgtattcca gtgcctgccg caggagggcg gcgcagtgac
     3241 gctgcgcggc gtgcgcagga tcgtgctcac cgcctcgggc ggtcctttcc gctccatgcc
     3301 gctcgagcgc ctgcacggcg tgacgcccca gcaggcctgc gcgcatccca actgggtgat
     3361 gggtcgcaag atctcggtcg actcggcgac catgatgaac aagggcctgg aggtcatcga
     3421 ggcctgctgg ctgttcggcc tgccggcgac ggcagtggag gtggtgatcc acgcgcagag
     3481 catcgtgcat tcgctggtcg agtacgtcga cggctcgctg atcgcccagc tgtccaaccc
     3541 ggacatgcgc gtgccgatcg cgcacgcgct gggattcccg gaccggctcg aatcgggcgc
     3601 gcgcgcgctc gacctcggcg cggtggggca gctgtcgtt
//
LOCUS       DASRIE010000097        21059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1166013, whole genome shotgun sequence.
ACCESSION   DASRIE010000097 DASRIE010000000
VERSION     DASRIE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166013"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFB53_04345"
     CDS             <1..1149
                     /locus_tag="VFB53_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HZN85999.1"
                     /translation="NLLAFAAREVPYYRERVPAGTPPGQWPLLDKAAVRAAGRALLAG
                     RRRGPLVPGSTSGTSGAPLPLWQDLAAINRENAFVWRQLEWAGLRRGERRAWMRGDMI
                     VPAAQREPPYWRMNRAENMLMLSSYHLAESSAAPYLAALAEFDPVLIQAYPSSIGFLA
                     AWMLASGQRYRGPALRGIVTSSETMPAARRRDIAVAFGCPVFDWYGQAERVAAAGTCE
                     HGGLHLLDDYSCVELLPAGDGWHELVGTGLNNYAMPLIRYRSGDLVRPAPAGRICPCG
                     RDFPLLERIAGRDDDAVRLPDGRVIGRLDHVFKGVGGIIEAQLRQERDGALTVLLVPG
                     PDFGAPARRALLGNLRERLGEELQIELRSVDAIPRGANGKFRAVVSAL"
     gene            1169..3199
                     /gene="asnB"
                     /locus_tag="VFB53_04350"
     CDS             1169..3199
                     /gene="asnB"
                     /locus_tag="VFB53_04350"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN86000.1"
                     /translation="MCGIAGALRLRPERSAPLPPEAAAGMLAALRLRGPDGEGEWRSA
                     DGLCWLGHRRLAIIDLQGGAQPMANEDGSVWTVLNGEIYNHLALRAELEALGHRFRTR
                     SDTEVLVHGYEAWGAQGLAVRLQGIFAFAVYDAARRSLALVRDPLGVKPLYWWSDGAQ
                     LLFASEIKALLRHPALRGRRVNRAGVAQMLVTRYVSRPATMFEGVFRLPEGCWMQVAA
                     GAAPPPLPQRYWDVRFRPEPIDEGEALVELDRLLRRTVSMQRMSEVPLGVQLSGGVDS
                     SLVTAMLDSLRREQGDPAPVQTFAVGFDVAQYSELAHARAVAEHCGTEHREITVGFRD
                     FAAQLPLLAWLYDEPMGEPPAIPTYLMCRAAKPHVTVMLCGEGADELFGGYSKYVFDG
                     FSAAFDWMPAAARALLLRGLASGMPFGGRRLRSMAEILSIDDAPRRYASWYGGFDTRL
                     QGSVLSARLRAEVGDGGLAEIFRALAGQCDSPHAFERFLYCDQHSRLVDDILVKGDRM
                     SMGAGIEARVPFLDHKLVEFAARLPRRMKVSGLRSKVLLKQLAARYLPPQVVYRRKVG
                     FTVPLTRWFAGPWRALLHDVLLSERCLDRGYCNPGVVRGILADHIARRVDREQGIWLL
                     LALELWHRLFVDDDGSEAAVARVQADFARSLGTLDRGALMPGVAVSAGVLGS"
     gene            3204..4208
                     /locus_tag="VFB53_04355"
     CDS             3204..4208
                     /locus_tag="VFB53_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86001.1"
                     /translation="MTDMTMRLRATTLATLLAAGASCALAQGSTLLFSSGFEQGTALR
                     PIDQGDCWATGCFQRFEGSDATTGFAWPPAIWGGGALLFLLADAPVDAATIGDYFSQQ
                     IVTATGRRGSPTRVLYSLTTQSGCCGADGQLGGATAANLLLAPLREEGDLYVSYWLKL
                     QPEAVPVRERGGLDPQDPQHWRFVFSWKTGSAVGIDDGDYRVILNIASWNRPGAPHPG
                     RPYWEVRGDNAAGADPNYVEFWRVENRGVPVPLGEWFKFEAFWHRSTGDDGRIWMAVN
                     GQVIADRAGPNRIARDINRIALSGVYSGEPYPLYQWLDDLQIWSGFPPECADPPCAPR
                     "
     gene            4193..5311
                     /locus_tag="VFB53_04360"
     CDS             4193..5311
                     /locus_tag="VFB53_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86002.1"
                     /translation="MRAALASAPGAAARRRIVVFAPSLQAVSGVSTHARMLLASELSR
                     EFELLHFQVGSEGRGENALQKLARLALSPLQLALFLLRTGADTVHLNASLDARGYWRD
                     LVYWAVARMLRRRVINQVHGGALPQEFFRGHALRTWLLRRFLVRSDAVTVLSSAELRA
                     YRAFDARIKVRLVPNAIDPAGLLDAARAAGAGGPLRLAYLGRLARAKGLLELVEALAL
                     MAGAGRDFTLRIGGGGPLEAELVAAVRAAGLAGRVHFLGTLAGADKARLWLDADVFAL
                     PSHFEGLPYALLEAMAAGCVPVTTRLAAIPDVVREGEHGLFVPPRDPRALARALARLD
                     DDRHALARMSAACRQRVREHYTMDRLVRDLRELYAGAE"
     gene            5316..7274
                     /locus_tag="VFB53_04365"
     CDS             5316..7274
                     /locus_tag="VFB53_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZN86003.1"
                     /translation="MSAGLAWRLHRLRAMRPAEIGWRLGSALGAALERTGIGLARDAG
                     PGADTVGAPWARPLPCGFDAAPTVAAAERLLAGRWDVLSLDDCLLGFPPRWNCDPSTG
                     RQAPLDYGKGIDVRDPRRVGDIKHLWEINRHLELVTLAQAWHLTGEARYAEGALRLLE
                     TWLEQCPYPRGAQWASALEAAVRLMNWATARQLLGAAAAPLTPRWAAAAFQHCHFVAR
                     NLSRHSSANNHLFGELTGLLLGALAWPRWPESRRWRELARAGLEAQAVTQNGADGVNR
                     EQAFWYHHEVADMMLLAFLHGRANGVHFSRRYTLRLEAMLEFIASVMDAGGQVPMVGD
                     ADDAVMVRFSRAAGFSPYRSLLATGAVLFDRGDFKAAAGGFDDKSRWLLGDAAAARYA
                     ALPADPARPPRLAFPEAGYYVLGADYGTPREVKALVDAGPLGYLSIAAHGHADALQMT
                     LSVAGREILVDPGTYAYHGEPRWRDYFRGTSAHNTLRVDGADQSVSGGPFLWREHAPA
                     RCSYFDVGVEADRFEGVHEGYRRLPDPVRHWRRIAFAKRERVFLVRDSLQCAREHEVE
                     VHWHLAEGCTARAAGHAVEIDCAPVRLRLECPRQLEPPAVVVGREHPPLGWVSRGLGR
                     KSPAPTVVCRGRAGAVSLETRISVVHLD"
     gene            7318..8631
                     /locus_tag="VFB53_04370"
     CDS             7318..8631
                     /locus_tag="VFB53_04370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZN86004.1"
                     /translation="MKLSVLGLGYVGTVSAACLAREGHEVLGVDAEPEKVTLINAGQS
                     PIIERDVGPMIREQVAAGRLRASLDVASAVAHAELLLVCVGTPSRANGDIELKYVRRV
                     CEQIGAALREHAGAPVVAIRSTLLPGTLRQVVIPLLEKASGKRAGAEFGLCLNPEFLR
                     EGSAVHDYFHPPKTVIGEVNRASGDLLAGLYAGIKAPLIRTDLETAEMVKYADNAWHA
                     LKVGYANEIGNLCKALGVDSHRVMDIFCQDTKLNLSSYYLKPGFAFGGSCLPKDLRAL
                     LYRAKSLDVTLPILASILPSNALQVERALQAVIDAGHREVGVLGFSFKAGTDDLRESP
                     VVELAERLIGKGYELRFYDENVNLARLHGANRDYILNRIPHISRLMVASVDEVLSHAG
                     TIVIGNAAAEFRDVPRRLRGTQSIVDLVRVSGARSVAGVYEGICW"
     gene            8628..9839
                     /locus_tag="VFB53_04375"
     CDS             8628..9839
                     /locus_tag="VFB53_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86005.1"
                     /translation="MTGAGRVLILVENLPVPFDRRVWQEACTLRDAGWVVSVICPTGK
                     GFEKRFEEIDGVNVYRYSLPLEAEGAAGYAIEYSVALFWSFLLSLRVLFTRGFDVVHA
                     CNPPDLFFLLGGFYKLFGKKFVFDHHDLNPELYEAKFGRRDFFHRLLLKLERWTFRAA
                     DVSIATNESYRKIAIERGGMDPDRVFVVRSGPSLERLRIQPPMEELKQGRRYLVGYVG
                     VMGRQEGIDLLLHAVRIVVRCFAREDVHFGLVGGGTSLSEMQMLAQNLSIEEYVTFTG
                     RVPDPQLLAMLNTADVCVNSDRAGALNDKSTMNKIMEYMALGKPVVQFDLTEGRRSAG
                     EASLYARQDDPADLAARILALLDDPERRARMGRLGRERVERELEWRHQAPRLLAAYGS
                     LWQGHALPAAR"
     gene            9817..10353
                     /locus_tag="VFB53_04380"
     CDS             9817..10353
                     /locus_tag="VFB53_04380"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZN86006.1"
                     /translation="MRFLPLDSPERLALAAGWLAREENHQWLDFGNGGQAISPALLKI
                     MTQRDAHFIRLYTSPEDDAPLGIAALNSVDRRFRSAVFWGVAGEKSFRRRGWATLASS
                     RFLSLAFRELGLNVIQTWVAEGNPSRRAVERLGFHFVGRQRQCHAIDGRPRDRLLYDL
                     LASEHRELPDTRPLRAAA"
     gene            10361..10840
                     /locus_tag="VFB53_04385"
     CDS             10361..10840
                     /locus_tag="VFB53_04385"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN86007.1"
                     /translation="MDPKVRQKTLRLISNGVYVLTARSGERLGAATVTWVSQISFRPP
                     LIMAAVRRESNVFQCLAESGSAVLHIVGDGQRDIAKRFFYPTRAEAGKLNGEPYAAGQ
                     TAAPVLARLPAHVECRVGRVIDAGGDHAMVILEAVEAECAEAVQPLTIAQTPWHYGG"
     gene            10837..12012
                     /locus_tag="VFB53_04390"
     CDS             10837..12012
                     /locus_tag="VFB53_04390"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZN86008.1"
                     /translation="MRALLAVLALAAGAAQAQEFPRLMGMNIGAKHYDDSLYQEQLAR
                     LDVAILGFYRGWNPRGTAASPGAAMRKVVQAMKARNPRLLAGQYTVLNEANDDPRDAA
                     TRDLRDKLGAEGWWLRDAARRRVQWTDRYANWETNFTPWAKPDGAGRRWPEWLAERNH
                     AVYFREVPEFDIVFLDNVMNPLRVKGDWDGDGANDDPKDAKVLAAHFAGHLAHWRRLR
                     ELEPRLLLLANVDHDLANPEWRGVLDGAFLEGLMGERWSLESWGGWEAMMRRYRAALA
                     NTRGPKLVGFNVHGDAKDARFFRYAYASCLLADGYFSFTDKAKGYGSVPWFKEYDIAL
                     GKAASPPPAAPWRDGVWRRDFERGVALVNPTGSAKTVELVPGRQLRLAAKDGIVLAR"
     gene            complement(11921..13657)
                     /locus_tag="VFB53_04395"
     CDS             complement(11921..13657)
                     /locus_tag="VFB53_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86009.1"
                     /translation="MLRIGLLIDGSELRWFDAELVRRIGADRIALVIQQELPRRSLAE
                     RLWRALKRGTLARALAFRAAVWLERRLTALRRPDIEELFRMVPLQEVCDTERLRLRPL
                     VSPSGFVHRFADEDVQKLKAWKLDVILRMGSGILRGEVLNAAMHGILSFHHGDNRTHR
                     GGPAGYWEVLERRDCTGYIVQRLTAALDAGDVLARGELATRPRYLLNQHHLYKCSLQT
                     MLDLLARLERDGALPREAPQPDWTARAPYTMPRVRDTLRYLGAQLGRALAGLARKLLV
                     QRNAWELRVFSGDWQRLAAARARALPVPRGRFWADPFIVRRGEEKVIFFEEYEFARDK
                     AHISALVGNAQGGFDYAGPVLRAPYHLSFPFVFRHQGQLFMCPETHAARRVELWRCAR
                     WPLEWRFERVLMEGVEACDSLIVEHGGRWWLLTNLERDGAGDYCRELHAFHADSPLAG
                     RWTPHAANPVVTGASRARNAGLLQRDGSLYRLAQAQGFDRYGRALKLFEVTQLTQQGY
                     QERQVAALEPQGGLLGIHHLSACEGTVVFDALRPRSFWAELSVPGRFRPSPRDATAVP
                     ARARPSSPIPSD"
     gene            complement(13662..14798)
                     /locus_tag="VFB53_04400"
     CDS             complement(13662..14798)
                     /locus_tag="VFB53_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86010.1"
                     /translation="MRLCFLGLENLPVLAPEYGRHNIAGEGVQQTLLARAFARRGHEV
                     RMVTWDYGQPEGAVWQGIRVHKAYAPDAGVPLLRFFHPRWSGMWSALARADAELYYTS
                     CAGMHLGLLALFCQRHGRRFVFRSASDTDCDPARLLVRYRRDQWLYEYGLRRADAILV
                     QSRSQAQALASNYSLPGRVAGMLVDAPAPAGTRDIDVLWASNIRRLKRPDRLLALAAR
                     LPQLRFHMLGGPLPGEEALYRETADAATARRNVTFHGRIAYREAAALYDRARLFVNTS
                     EVEGFPNSYLQAWVRGVPVVTLIDPDDVIAREGLGFAASTPAGIEGAVRGLLEDKAAW
                     QAVSARCRAYMAREHGEDRVLAPYVEAFDEALRGTGAALRIAGA"
     gene            complement(14892..17411)
                     /locus_tag="VFB53_04405"
     CDS             complement(14892..17411)
                     /locus_tag="VFB53_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HZN86011.1"
                     /translation="MLSALLRTAFALFSFLIVLALPAVAGAGLTETYGRLPLHFEENR
                     GQAHKDVRFLARGPGYGLYLTAGEAVLALGAKDRTVVRMALVGANADALARGLEELPG
                     KANYFIGKDPAKWRTNVPTYAKVHYREVYDGIDLVYYGNQRQLEYDFVVAPGADPEKI
                     LLRFEGATAIKIDAQGNLVLHTASGELRQHKPVIYQEVEGVRQEIEGGYVIKDAHRVG
                     FQVAAYDRGEPLVIDPVLAYATYLGGSQRDLGVGIAVDAAGNAYVVGETGSVDFPTTI
                     GAHQMIYGGTGGPFSTGDAFVSKLNSTGTALVYSTYLGGSLRDNGSAIAVDAGGNAYV
                     TGSTDSHDFPVTAGAYQQTHRSMTDAFVTKLGPTGSTLAYSTFIGGDNLDQAYAIAVD
                     AAGSAYITGNTDSIDFPTTALAFQPHHAGADFSGDAFVTKLNSAGSALVYSTYLGGSG
                     DDDWGQGIAVDASGNAYVVGSTPSTDFPTTPGAQQPVSTQGGGFVTKLEPAGSALVYS
                     TYLKGSAAAVAVDASGNAYVTGATDGSDFPTTPGAFQTMSGGSTNAYVAKLNAAGSAF
                     VYSTLLGGPGGAVGRGIAIDAVGRVHVTGDAYMVCGPFSSCVTGFPTTPGAFQPDHGG
                     SASDAFVSTLDPTGSALVYSTYIGGSGADSGLGIAVDTSGGTYITGWTNSGNFPATVG
                     ALQPVSGGDDDGFVAKFQIDGPTVTRFEEDTATYTGYWPPYGPETGTFSGGTIRASNQ
                     ATATATFTFTGTAVSWIGVKCNACGIATAQIDGGAPTVVDTFGPGEPGSLTSEPVFTA
                     SGLAPGATHTLTIVVMGGGASIPGSLTGSAHVAVDAFDVTK"
     gene            complement(17456..18277)
                     /locus_tag="VFB53_04410"
     CDS             complement(17456..18277)
                     /locus_tag="VFB53_04410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN86012.1"
                     /translation="MILLRGRAATYGEVWFGEEPSPDATVDIVQYRFRSEPVAKARIA
                     PLLTMVTDLTEDEEAIAAHFGKDCRYKVRRADTKDGLRLEFSAAPESRLEEFAKFFDA
                     FAKQKGHSPCDRHWLEAACVAEQLALSCATNDAGPLVWHAHVLCGQTAGLQYTASHFR
                     GQESDVRALVGRANRWLHWKDLLALKAMGFERYDWGGLFEDESTPEREGINRFKRDFG
                     GEQLRVYECTAPASLRGRVYLPLRDAWRRGWTRKTDYAPGPDGLRAERPHTPITS"
     gene            complement(18279..19232)
                     /locus_tag="VFB53_04415"
     CDS             complement(18279..19232)
                     /locus_tag="VFB53_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HZN86013.1"
                     /translation="MPFIVTIDTEGDDLWARPREITTRNAAHLPRFQALCERFGFKPV
                     YLTNYEMAMNGEFVDFARDVLARSAGEVGMHLHAWNSPPLFSLTGDDFHHQPFLIEYP
                     DEVMREKIHFMTRLLENRFGRNVVSHRAGRWAMDGRYAAMLVDEGYRVDCSVTPGVDW
                     SPNLGDPRGGGGADYRGFPQRPYFVDPRDISVPAAGPLMEVPVTVRSGTLYRIAPWIY
                     RVPLLRGAANRLSRSLNWLCPAESGLDEMLQVARIARDEGAAHLEFMLHSSELMPGGS
                     PRFRSADDIEKLYERLELLFTNLRVWCRGMTLREFHAAASA"
     gene            19501..21039
                     /locus_tag="VFB53_04420"
     CDS             19501..21039
                     /locus_tag="VFB53_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86014.1"
                     /translation="MYTVRASAIALLFLSAAGNVVAQARDPVVWPFASTSMWNMPIGS
                     NANYVAINMPIPEDPGFPPSIPPTNWAPMPGIHDEYIVMAPTAPMTDIYYSNVGWSGG
                     NRCAPTGIPPILLTQVPVPTNYVVDNGGGDNSAAFLMPDGRTLIQSQPFTRCLAGGPA
                     TSIIHFDYVDLYGDGRWGAHGGSQLSSVGGSIHVGELRPGIPVRHALAMTLNTTAVLA
                     PCDAMDDCYRWPAYSGDAGATNPTTGYGRMNPNPVSGMKMGALLAIPANVDVENGLGL
                     ESGPGKMLAWTLQNYGVYIVDSSGGPSIFIDADTGPNGSFAGSKREEFFNDFGMPFEQ
                     RVNSDTPWSRDMQKLIQALHLVDNNGPSSIGGGGTPLQPLAPALAVTRIEEDNSTVTF
                     SPSGGWVERQREVAGFNDGRALSYDLSGGTATFSFTGTGVSWIGLKCNVCGIASVSID
                     GGAPTMVNTAGPAGPGSFGLRSEPVFTASGLVAGPHTLQIAVTGTTSSGGSHIAVDAF
                     DVTP"
BASE COUNT         3123 a         7232 c         7490 g         3214 t
ORIGIN      
        1 aacctgctcg ccttcgccgc gcgcgaggtg ccgtactacc gcgagcgcgt accggcgggc
       61 accccgcccg ggcaatggcc gctgctcgac aaggccgcgg tgcgcgccgc cggccgcgcg
      121 ctgctcgccg gccgccggcg cggaccgctg gtgccgggga gcaccagcgg caccagcggc
      181 gcgcccctgc cgctgtggca ggacctggcc gccatcaacc gcgagaacgc cttcgtctgg
      241 cgccaactgg agtgggccgg gctgcggcgc ggcgagcgcc gcgcgtggat gcgcggcgac
      301 atgatcgtgc ccgccgcgca gcgcgagccg ccgtactggc gcatgaaccg cgcggagaac
      361 atgctgatgc tgtcctccta ccacctggcc gagtcgagcg ccgcgcccta cctcgcggcg
      421 ctcgccgaat tcgacccggt gctgatccag gcctacccgt cctccatcgg attcctcgcc
      481 gcctggatgc tcgccagcgg ccaacgttat cgcggccccg cgctgcgcgg catcgtgacc
      541 tcctccgaga cgatgcccgc ggcgcggcgc cgcgatatcg ccgtcgcctt cggctgcccg
      601 gtgttcgact ggtacgggca ggccgagcgc gtcgccgcag ccggcacctg cgagcacggg
      661 ggcttgcacc tgctggacga ctactcctgc gtcgagctgc tcccggccgg cgacggctgg
      721 cacgagctgg tgggcaccgg cctgaacaat tacgccatgc cgctgatccg ctaccgcagc
      781 ggcgacctgg tgcgcccggc gcccgcgggg cgcatctgcc cctgcgggcg cgacttcccg
      841 ttgctcgaga gaatcgccgg ccgcgacgac gatgcggtgc ggctgcccga cggccgcgtg
      901 atcgggcgcc tggaccacgt gttcaagggc gtcggcggca tcatcgaggc gcagctccgc
      961 caggagcgcg acggtgccct gaccgtgctg ctggtgcccg gcccggattt cggcgcgccg
     1021 gcgcggcgcg cgctgctcgg caatctgcgc gagcggctgg gcgaggagct gcagatcgag
     1081 ttgcgcagcg tcgacgcgat cccgcgcggc gccaacggca agttcagggc cgtggtgagc
     1141 gcgctctgaa ccggcgcggc ccccgaccat gtgcggcatc gccggcgcgc tgaggctgcg
     1201 cccggagcgc agcgcgccgc tgccgccgga ggccgcggcg ggcatgctcg ccgcactgcg
     1261 gctgcgcggc ccggacggcg aaggcgagtg gcgctccgcc gacggcctgt gctggctcgg
     1321 ccaccgccgc ctcgccatca tcgacctgca gggcggcgcg cagccgatgg ccaacgagga
     1381 cggcagcgtg tggaccgtgc tcaacggcga gatctacaac cacctcgcgc tgcgcgccga
     1441 actggaggcc ctcggccacc gctttcgcac gcgcagcgac accgaggtgc tggtgcacgg
     1501 ctacgaagcc tggggcgcgc agggactggc cgtgcgcctg cagggcatct tcgccttcgc
     1561 cgtgtacgac gccgcgcggc gcagcctcgc cctggtgcgc gacccgctgg gcgtaaagcc
     1621 gttgtactgg tggagcgacg gcgcccagct gctgttcgcg agcgaaatca aggcgctgct
     1681 gcgccacccg gccctgcgcg ggcgccgggt caaccgcgcg ggcgtcgcgc agatgctggt
     1741 gacgcgctac gtgtcgcgcc ccgccaccat gttcgaaggc gtgttccgcc tgcccgaggg
     1801 ttgctggatg caggtggcgg cgggcgccgc gccgccgccc ctgccgcagc gctactggga
     1861 cgtgcgcttc cggccggagc ccatcgacga gggcgaggcg ctggtcgagc tcgatcggct
     1921 gctgcggcgc acggtgagca tgcagcgcat gtccgaggtg ccgctcggcg tgcagctctc
     1981 cggcggggtc gacagcagcc tggtgaccgc gatgctggac agcctgcggc gcgagcaggg
     2041 cgacccggcg ccggtgcaga ccttcgcggt gggcttcgac gtggcgcagt acagcgagct
     2101 ggcgcacgcg cgcgcggtgg ccgagcactg cggcaccgag caccgcgaga tcacggtggg
     2161 cttccgcgat ttcgccgcgc agctgccgct gctcgcctgg ctgtacgacg agccgatggg
     2221 cgagccgccc gccatcccca cctacctgat gtgccgcgcg gccaagccgc acgtcacggt
     2281 gatgctgtgc ggcgaaggcg cggacgagct gttcggcggc tactccaagt acgtgttcga
     2341 cggcttctcg gcggccttcg actggatgcc ggcggcggcg cgcgcgctgc tgctgcgcgg
     2401 cctggcgagc ggcatgccgt tcggcggccg gcgcctgcgc agcatggccg agatcctctc
     2461 gatcgacgac gccccgcgcc gctacgcctc ctggtacgga ggcttcgaca cccgcctgca
     2521 gggcagcgtg ctgtcggcgc gcctgcgcgc cgaggtgggc gacggcggcc tggcggagat
     2581 cttccgcgcg ctggccgggc aatgcgacag cccgcacgcc ttcgagcgct tcctgtactg
     2641 cgaccagcat tcccgcctgg tcgacgacat cctggtgaag ggcgaccgca tgagcatggg
     2701 cgccggcatc gaggcgcgcg tgccgttcct cgaccacaag ctggtggaat tcgccgcgcg
     2761 cctgccgcgg cgaatgaagg tgagcggcct gcgctcgaag gtgctgctca agcagctggc
     2821 ggcgcgctac ctcccgccgc aggtggtgta ccgccgcaag gtcggcttca ccgtgccgct
     2881 gacgcgctgg ttcgccggcc cttggcgcgc gctgctgcac gacgtgctgc tgtccgagcg
     2941 ctgcctggac cgcggctact gcaatcccgg cgtggtgcgc ggcatcctcg ccgaccacat
     3001 cgcgcggcgc gtggaccgcg agcagggcat ctggctgctg ctggcgctgg aactgtggca
     3061 ccgcctgttc gtggacgacg acggcagcga ggcggcggtg gcgcgggtgc aggccgactt
     3121 tgcccgctcg ctgggcactc tggaccgcgg cgccctgatg ccgggggttg cggtgagcgc
     3181 aggagtcctc gggagctgag gccatgaccg acatgaccat gcgattgcgc gcgaccacgc
     3241 tcgccaccct gctcgccgcg ggcgccagct gcgccctggc gcaaggcagc accctgctgt
     3301 tcagctcggg cttcgagcag gggacggcgc tgcgcccgat cgaccagggc gactgctggg
     3361 caaccgggtg cttccagcgc ttcgagggca gcgacgcgac caccggtttt gcatggcctc
     3421 ccgcgatctg gggcggcggc gcgctgctgt tcctgctcgc cgatgcgccg gtggatgcgg
     3481 ccacgatcgg cgactacttc tcccagcaga tcgtgaccgc gacggggcgc aggggctcgc
     3541 ccacgcgcgt cctctacagc ctgaccacgc aaagcggctg ctgcggcgcc gacggccagc
     3601 tcggcggcgc tacggcggcc aatctgctgc tcgcgccgct gcgcgaggag ggcgacctgt
     3661 acgtgagcta ctggctcaag ctgcaaccgg aggcggtgcc ggtgcgcgag cgcggcgggc
     3721 tggacccgca ggacccgcag cactggcgct tcgtgttctc gtggaagacc ggcagcgccg
     3781 tgggcatcga cgacggcgac tatcgcgtga tcctcaacat cgcgagctgg aaccggcccg
     3841 gcgcgccgca tcccggcagg ccgtactggg aagtccgcgg cgacaacgcg gcgggcgccg
     3901 acccgaacta cgtggagttc tggcgcgtgg agaaccgcgg cgtcccggtg ccgctgggcg
     3961 aatggttcaa gttcgaggcg ttctggcatc gcagcacggg agacgacggc cgcatctgga
     4021 tggcggtgaa cggccaggtg atcgccgatc gcgccgggcc gaaccgcatt gcccgcgaca
     4081 tcaaccgcat cgccctgtcc ggcgtctaca gcggcgagcc ttacccgctc taccagtggc
     4141 tggacgatct gcagatctgg agcgggttcc cgccggagtg cgcggatccc ccgtgcgcgc
     4201 cgcgctagcg tcggccccgg gcgccgcggc ccggcgcagg atcgtcgtgt tcgcgccctc
     4261 gctgcaggcg gtgagcggcg tgtccacgca cgcgcgcatg ctgctcgcgt ccgagctgtc
     4321 gcgcgaattc gagctgctgc atttccaggt cggcagcgag ggccgcggcg agaacgcgct
     4381 gcagaagctc gcgcgcctcg ccctcagccc gctgcagctc gcgctgttcc tgctgcgcac
     4441 cggcgcggac accgtgcacc tgaacgcatc gctcgatgcc aggggctact ggcgcgatct
     4501 ggtctactgg gcggtcgcgc ggatgctgcg ccgccgggtg atcaaccagg tgcacggcgg
     4561 cgcgctgccg caggagttct tccgcggcca cgcgctgcgc acctggctgc tgcggcgctt
     4621 cctggtgcgc agcgacgcgg tgacggtgct ctccagcgcc gagctgcgcg cctaccgggc
     4681 gttcgatgcg cgcatcaagg tgcgcctggt gcccaacgcc atcgacccgg cgggcctgct
     4741 cgacgcggcg cgcgccgccg gcgccggcgg gccgctgcgc ctcgcctacc tggggcgcct
     4801 ggcgcgcgcc aagggcctgc tggagctggt ggaggcgctc gccctgatgg cgggcgccgg
     4861 gcgcgacttc acgctgcgca tcggcggcgg cgggccgctg gaggccgagc tcgtcgccgc
     4921 ggtgcgcgcc gcgggcctcg ccgggcgcgt gcacttcctc ggcaccctcg ccggcgcgga
     4981 caaggcgcgc ctgtggctgg acgccgacgt gttcgcgctg ccctcgcatt tcgaggggct
     5041 gccctacgcg ctgctcgagg cgatggcggc gggctgcgtg ccggtgacca cgcgcctcgc
     5101 cgccattccc gacgtggtgc gcgagggcga gcacggcctg ttcgtgccgc cgcgcgatcc
     5161 gcgcgcgctg gcgcgtgcgc tggcgcggct ggacgacgac cgccatgcgc tcgcgcgcat
     5221 gtcggcggcc tgccgccagc gcgtgcgcga gcactacacc atggaccggc tggtgcgcga
     5281 cctgcgtgag ctgtacgcgg gcgcggagtg aaggcgtgag cgcgggactc gcctggcggc
     5341 tgcaccggct gcgcgccatg cggcccgccg aaatcggctg gcgcctcggc agcgcgctcg
     5401 gcgcggcgct cgagcgcacc ggcatcggcc tggcgcgcga cgcgggcccg ggcgccgaca
     5461 ctgtcggggc gccctgggcc aggccgctgc cgtgcggctt cgatgcggcg ccgacggtcg
     5521 ccgccgccga gcgcctcctc gccggacgct gggacgtgct ttcgctcgac gactgcctgc
     5581 tcggctttcc gccgcgctgg aactgcgacc cgagtaccgg acggcaggcg ccgctggact
     5641 acggcaaggg catcgacgtc cgggacccgc ggcgggtcgg cgacatcaag catctgtggg
     5701 aaatcaaccg gcacctcgag ctcgtgacgc tggcgcaggc ctggcacctg acgggcgaag
     5761 cgcgctacgc cgagggcgcg ctgcgcctgc tggagacctg gctggagcag tgtccctatc
     5821 cgcgcggcgc gcagtgggcg agcgcgctcg aggcggcggt gcgcctgatg aactgggcca
     5881 ccgcccggca gctgctcggc gccgcggcgg cgccgctcac gccgcgctgg gcggccgcgg
     5941 cgttccagca ctgccatttc gtcgcgcgca acctgtcgcg ccactcctcg gcgaacaacc
     6001 acctgttcgg cgagctcacc gggctgctgc tcggcgcgct cgcctggccg cgctggcccg
     6061 agtcgcgccg ctggcgcgag ctggcgcgcg ccgggctgga ggcgcaggcg gtgacgcaga
     6121 acggcgcgga cggggtgaac cgcgagcagg cgttctggta ccaccacgag gtcgccgaca
     6181 tgatgctgct cgccttcctg cacgggcgcg ccaacggcgt gcatttttcg cgccgctaca
     6241 cgttgcggct ggaagcaatg ctcgaattca tcgcctcggt gatggacgcc ggcggccagg
     6301 tgccgatggt gggcgacgcc gacgacgcgg tgatggtgcg cttttcgcgg gccgccggtt
     6361 tctcgcccta ccggtcgctg ctcgccaccg gcgccgtgct gttcgaccgc ggcgacttca
     6421 aggccgccgc cggcggcttc gacgacaaga gccgctggct gctcggggac gccgccgcgg
     6481 cgcgctacgc ggcgctgccg gcggacccgg cgcgcccgcc gcggctcgcc ttccccgagg
     6541 ccggctacta cgttctcggc gcggactacg gcacgccgcg cgaagtgaag gcgctggtgg
     6601 acgcaggtcc cctgggctac ctgtcgatcg ccgcgcacgg ccatgccgac gcgctgcaga
     6661 tgacgctgtc ggtggccggg cgcgaaatcc tcgtcgaccc cggcacctat gcctaccacg
     6721 gcgagccgcg ctggcgcgac tacttccgcg gcacctcggc gcacaacacg ctgcgcgtgg
     6781 acggcgccga ccagtcggta tccggcgggc cgttcctctg gcgcgagcac gcgcccgctc
     6841 gctgcagtta cttcgacgtc ggggtggaag cggaccggtt cgagggcgtg cacgagggct
     6901 accggcgcct ccccgatccg gtgcggcact ggcggcgcat cgcgttcgcc aagcgcgagc
     6961 gggtgttcct cgtgcgcgac agcctgcagt gcgcgcgcga gcatgaggtg gaggtccact
     7021 ggcacctcgc cgagggctgc acggcgcgcg ccgccgggca cgcggtcgag atcgactgcg
     7081 cgcccgtgcg cctgcgcctg gaatgcccgc gccagctcga gccgcccgcg gtggtcgtgg
     7141 ggcgcgagca cccgcccctg ggctgggtgt cgcgcgggct gggacgcaag tccccggcgc
     7201 cgactgtcgt ctgccggggc agggcgggcg cggtgagcct ggagacgcga atttcagtgg
     7261 tccacttgga ctaggtcccc ccggatgggc gtcaaggggc ctgcctagca tggggccatg
     7321 aagctgagcg tcctgggcct cggctacgtc ggcaccgtct ccgcggcctg cctcgcccgc
     7381 gagggccacg aagtgctcgg cgtggacgcc gagccggaaa aggtcacgct gatcaacgcc
     7441 ggccagagtc cgatcatcga gcgcgacgtc gggccgatga tccgcgagca ggtggcggcg
     7501 ggccggctgc gcgcgtcgct ggacgtcgcc tcggcggtgg cccatgccga gctcctgctg
     7561 gtgtgcgtgg gcaccccgag ccgcgccaac ggcgacatcg agctgaagta cgtgcgccgc
     7621 gtctgcgagc agatcggcgc cgcgctgcgc gagcacgccg gcgcgccggt tgtggcgatc
     7681 cgcagcacgc tgctgcccgg caccctgcgc caggtggtga ttccgctgct ggaaaaggcc
     7741 tcgggcaagc gcgcgggcgc cgagttcggc ctgtgcctca acccggagtt cctgcgcgaa
     7801 ggcagcgccg tgcacgacta cttccacccg ccgaagaccg tgatcggcga ggtgaaccgc
     7861 gccagcggcg acctgctcgc cggcctgtac gcggggatca aggcgccgct catccgcacc
     7921 gacctggaga ccgccgagat ggtgaagtac gccgacaacg cctggcacgc gctgaaagtg
     7981 ggctacgcca acgagatcgg caacctgtgc aaggcgctgg gcgtggacag ccaccgcgtg
     8041 atggacatct tctgccagga cacgaagctc aacctgtctt cctattacct caagcccggc
     8101 ttcgccttcg gcggctcctg cctgcccaag gacctgcgcg cgctgctgta ccgcgcgaag
     8161 tcgctggatg tcacgctgcc gatcctcgcc tcgatcctgc ccagcaacgc gctgcaggtc
     8221 gagcgcgcgc tgcaggcggt gatcgacgcg ggccaccgcg aggtgggcgt gctcggcttt
     8281 tccttcaagg cgggcaccga cgacctgcgc gagagcccgg tggtcgagct cgccgagcgc
     8341 ctgatcggca agggctacga gctgcgcttc tacgacgaga acgtgaatct cgcgcgcctg
     8401 cacggcgcca accgcgacta catcctcaac cgcatcccgc acatctcgcg actgatggtg
     8461 gcgagcgtgg acgaggtgct ctcgcacgcc ggcaccatcg tcatcggcaa cgcggcggcg
     8521 gaattccgcg acgtgccgcg gcgcctgcgc ggcacgcaga gcatcgtcga cctggtgcgg
     8581 gtgagcgggg cgaggagcgt cgcgggagtg tacgagggga tctgctggtg acgggcgccg
     8641 ggcgcgtcct gatcctggtg gagaacctgc ccgtgccctt cgaccggcgc gtgtggcagg
     8701 aggcctgcac gctgcgcgac gcgggctggg tcgtgtcggt gatctgcccg actggcaagg
     8761 gattcgagaa gaggttcgag gaaatcgacg gggtcaacgt ctaccgatac agccttccat
     8821 tggaagccga aggcgccgcg ggctacgcca tcgagtacag cgttgcgctg ttctggagtt
     8881 ttctgttgag cctgcgcgtg ctcttcacgc gcggcttcga cgtggtgcac gcctgcaacc
     8941 cgccggacct gtttttcctc ctcggcggct tctacaagct gttcgggaaa aagttcgtct
     9001 tcgaccacca cgatctgaac ccggagctct acgaggcgaa gttcggccgg cgcgacttct
     9061 tccaccgcct gctgctgaaa ctcgagcgct ggaccttccg cgccgccgac gtgtcgatcg
     9121 ccaccaacga gtcctaccgg aagatcgcca tcgagcgcgg cggcatggac cccgatcgcg
     9181 tgttcgtggt gaggagcggc ccgagcctcg agcgcctgcg catccagccg cccatggagg
     9241 aactcaagca gggccgccgc tacctcgtcg gctacgtcgg ggtgatgggc cgccaggagg
     9301 gcatcgacct cctgctgcac gcggtgcgca tcgtggtgcg ctgcttcgcg cgcgaggacg
     9361 tgcacttcgg cctggtgggc ggcggcacct cgctctccga aatgcagatg ctcgcgcaga
     9421 acctgagcat cgaggagtac gtcactttca ccgggcgcgt gccggacccg cagctgctcg
     9481 ccatgctcaa cacggcggat gtgtgcgtga actccgaccg cgcgggcgcg ctgaacgaca
     9541 agtccaccat gaacaagatc atggagtaca tggcgctcgg caagcccgtg gtgcagttcg
     9601 acctcaccga aggccggcgc tcggcgggcg aggcttccct gtatgcgcgg caggacgacc
     9661 cggcggacct cgccgccagg atcctcgcgc tgctcgacga cccggagcgc cgcgcgcgca
     9721 tggggcgtct cggccgcgag cgcgtggagc gcgagctcga gtggcgccac caggcgccgc
     9781 gcctgctcgc ggcctacggc tccctgtggc aaggccatgc gcttcctgcc gctcgatagc
     9841 cccgagcgcc tcgcgctcgc cgccggctgg ctggcgcgcg aggagaacca ccagtggctc
     9901 gatttcggca atggcgggca ggcgatctcg cccgcgctgc tgaagatcat gacgcagcgc
     9961 gacgcgcact tcatccgcct gtacacctcg ccggaggacg acgcgccgct cggcatcgcc
    10021 gccttgaaca gcgtggaccg gcgtttccgc agcgcggtgt tctggggcgt ggcgggggag
    10081 aaatcctttc gccgccgcgg ctgggccacg ctcgccagct cgcgcttcct gtcgctcgcc
    10141 ttccgcgagc tgggtctcaa cgtgatccag acctgggtgg ccgagggcaa tccctcgcgg
    10201 cgcgcggtcg agcgcctggg cttccacttt gtaggccgcc agcggcagtg ccacgccatc
    10261 gacggccggc cgcgcgaccg cctgctctac gacctgctcg ccagcgagca ccgcgagctg
    10321 cccgacacca ggccgctgcg ggccgccgcc tgaggccgcc atggacccca aggtccggca
    10381 aaagaccctg cgcctgatct ccaacggcgt gtacgtgctg accgcgcgct cgggcgagcg
    10441 actgggcgcg gccaccgtca cctgggtgtc gcagatctcc ttccgcccgc cgctgatcat
    10501 ggcggcggtg cggcgcgaga gcaacgtgtt ccagtgcctc gccgagagcg gcagcgcggt
    10561 gctgcacatc gtgggcgacg ggcagcgcga catcgccaag cgcttcttct atcccacgcg
    10621 cgccgaggcg gggaaactca acggcgaacc ctacgccgcg ggccagacgg cggcgccggt
    10681 gctcgcgcgc ctgcccgccc acgtcgaatg ccgcgtcggg cgcgtgatcg acgccggcgg
    10741 cgaccacgcc atggtgatcc tggaggcggt ggaggccgaa tgcgcggagg cggtgcagcc
    10801 gctcaccatc gcccagacgc cctggcacta cggcggatga gggcgctcct cgccgtgctg
    10861 gcgctggcgg cgggcgccgc ccaggcgcag gagttcccgc gcctgatggg catgaacatc
    10921 ggcgccaagc actacgacga ttccctgtac caggagcagc tcgcgcgcct cgacgtggcg
    10981 atcctggggt tttaccgcgg ctggaatccg cgcggcaccg ccgcgagccc gggcgcggcg
    11041 atgcgcaagg tggtgcaggc gatgaaagcg cggaatccgc ggctgctggc gggccagtac
    11101 acggtgctga acgaggcgaa cgacgacccg cgcgacgccg ccacgcgcga cctgcgcgac
    11161 aagctgggcg cggagggctg gtggctgcgc gacgccgccc ggcgccgggt gcagtggacc
    11221 gaccgctacg cgaactggga gaccaacttc acgccctggg cgaagcccga cggcgccggg
    11281 cggcgctggc cggagtggct cgccgagcgc aaccacgcgg tgtacttccg cgaggtgccg
    11341 gaattcgaca tcgtgttcct cgacaacgtg atgaacccgc tgcgcgtgaa gggcgactgg
    11401 gacggggacg gggccaacga cgacccgaag gacgccaagg tgctggcggc gcatttcgcc
    11461 gggcacctgg cgcactggcg gcgcctgcgc gagctcgagc cccgcctgct gctcctcgcc
    11521 aacgtcgatc acgacctcgc gaatccggaa tggcgcgggg tgctcgacgg cgccttcctc
    11581 gaggggctga tgggcgagcg ctggtcgctg gagagctggg gcggctggga ggcgatgatg
    11641 cggcgctacc gcgcggcgct cgccaacacg cgggggccga agctcgtcgg cttcaacgtg
    11701 cacggcgacg cgaaggatgc gcgctttttc cgctacgcct acgcctcctg cctgctggcg
    11761 gacggctact tctccttcac cgacaaggcg aagggctacg gcagcgtgcc gtggttcaag
    11821 gagtacgaca tcgcgctggg caaggcggcc tcgccgccgc cggcggcgcc ctggcgcgac
    11881 ggcgtgtggc ggcgcgattt cgagcgcggc gtggcgctcg tcaatccgac gggatcggcg
    11941 aagacggtcg agctcgtgcc gggacggcag ttgcgtctcg cggcgaagga cggaatcgtc
    12001 ctggcacgct gagctccgcc cagaaggaac gcggccgcag cgcgtcgaac accaccgttc
    12061 cctcgcaggc cgacaggtgg tggatgccca gcaagccgcc ctgcggctcg agcgccgcga
    12121 cctggcgctc ctggtagccc tgctgggtga gctgcgtgac ctcgaacagc ttcagcgcgc
    12181 gcccgtagcg atcgaagccc tgcgcctgcg cgaggcggta gaggctgccg tcgcgctgca
    12241 acaggcccgc attgcgcgcg cggctcgcgc cggtgaccac cgggttcgca gcgtgcggcg
    12301 tccagcgccc ggcgagcggc gaatcggcat ggaaagcgtg cagctcgcgg cagtagtcgc
    12361 ccgcgccgtc gcgctcgagg ttggtgagca gccaccagcg cccgccgtgc tcgacaatga
    12421 ggctgtcgca ggcctcgacg ccttccatca gcacgcgctc gaagcgccac tcgagcggcc
    12481 agcgcgcgca gcgccacagc tcgacgcgcc gcgcggcgtg cgtctccggg cacatgaaca
    12541 actggccctg gtggcggaac acgaagggga agctcaggtg gtagggcgcg cgcagcaccg
    12601 gcccggcgta gtcgaagccg ccttgcgcgt tgcccaccag ggcggagatg tgcgccttgt
    12661 cgcgcgcgaa ctcgtactcc tcgaagaaga tcaccttctc ctccccgcgg cgcacgatga
    12721 acggatcggc ccagaagcgg ccgcgcggca cgggcagggc gcgcgcgcgc gccgccgcga
    12781 ggcgctgcca gtcgcccgag aacacgcgca gctcccaggc gttcctctgc acgaggagct
    12841 tgcgcgccag tccggcgagc gcgcggccga gctgcgcgcc gaggtagcgc agcgtgtcgc
    12901 gcacgcgcgg catcgtgtac ggcgcgcgcg cggtccagtc cggctgcggc gcctcgcgcg
    12961 gcagcgcgcc gtcgcgctcc agccgcgcga gcaggtcgag catggtctgc agcgagcact
    13021 tgtacaggtg gtgctggttg agcaggtagc gcggccgcgt ggccagctcg ccgcgcgcca
    13081 gcacgtcccc cgcgtccagc gccgcggtga gccgctgcac gatgtagccg gtgcagtcgc
    13141 gccgctcgag cacttcccag tagccggccg ggccgccgcg gtgcgtgcgg ttgtcgccgt
    13201 ggtggaagga caggatgccg tgcatcgccg cgttgaggac ctcgccgcgc aggatccccg
    13261 aacccatgcg caggatcacg tcgagcttcc aggccttcag cttttggacg tcctcgtcgg
    13321 cgaagcggtg cacgaagccc gagggggaga cgagcgggcg cagccgcagg cgctcggtgt
    13381 cgcagacctc ctgcagcggc accatgcgga acagctcctc gatgtcgggc cggcgcagcg
    13441 cggtgaggcg gcgctccagc cacaccgcgg cgcggaaggc gagcgcgcgc gcgagcgtgc
    13501 cgcgcttcag cgcgcgccac aggcgctcgg cgaggctgcg ccggggcagc tcctgctgga
    13561 tcaccagcgc gatgcggtcg gcgccgattc tccgcaccag ctcggcgtcg aaccagcgca
    13621 gctccgaccc gtcgatgagg aggccgatgc gcagcacggc ttcaggcccc ggcgatgcgc
    13681 agcgcggcgc ccgtcccgcg cagcgcctcg tcgaaagcct cgacgtaggg ggcgagcacc
    13741 cggtcctcgc catgctcgcg cgccatgtag gcgcggcagc gagcgctgac cgcctgccag
    13801 gcggccttgt cctccaggag gccccgcacc gcgccttcga tgccggcggg cgtgctggcg
    13861 gcgaagccca ggccctcgcg cgcgatgacg tcatccgggt cgatgagcgt caccaccggc
    13921 acgccgcgca cccaggcctg caggtaggag ttggggaacc cttccacctc ggaagtgttg
    13981 acgaacaggc gcgcgcggtc gtagagcgcg gcggcctcgc ggtaggcgat gcggccgtgg
    14041 aaggtgacgt tgcgccgcgc cgtggcggcg tccgcggtct ctcggtacag cgcctcttcg
    14101 cccggaagcg gcccgcccag catgtggaag cgcagctgcg gcaggcgcgc ggcgagcgcg
    14161 agcagccggt cggggcgctt caggcgccgg atgttgctcg cccagagcac gtcgatgtcg
    14221 cgcgtgccgg ccggcgccgg cgcatccacc agcatgccgg cgacccggcc ggggaggctg
    14281 tagttgctcg ccagcgcctg cgcctgcgac cggctctgca ccaggatggc gtcggcgcgc
    14341 ctgaggccgt attcgtacag ccactggtcg cgccggtagc gcaccagcag gcgcgccggg
    14401 tcgcagtcgg tgtcgctcgc gctgcggaac acgaagcgcc tgccgtggcg ctggcagaag
    14461 agggcgagca gccccaggtg catgccggcg cagctggtgt agtagagctc cgcgtcggcg
    14521 cgcgccagcg ccgaccacat tcccgaccag cgcgggtgga agaagcgcag cagggggact
    14581 cctgcgtccg gggcgtaggc cttgtgcacg cggatgccct gccacacggc gccttcgggc
    14641 tggccgtagt cccaggtcac catgcgcacc tcgtgcccgc ggcgcgcgaa ggcgcgggcg
    14701 agcagcgtct gctgcacgcc ttcgccggcg atgttgtgcc ggccgtactc gggggcgagg
    14761 accgggaggt tctccaggcc gaggaaacag aggcgcacga tgccctcagg ctagcggggc
    14821 ctcattgagg tgcgacgggg ggccgggggg cccccctcta gtccgatcgg ctcaggggcg
    14881 gcgccgggcg atcactttgt cacgtcgaag gcgtccaccg ccacgtgggc gctgccggta
    14941 agggagccgg gaatcgaggc accgccgccc atcacgacaa tcgtgagcgt gtgtgtcgcg
    15001 ccgggcgcaa gccccgaggc ggtgaagacg ggctcggagg tgaggctgcc gggttccccg
    15061 ggaccgaagg tgtccacgac cgtcggcgcg ccgccgtcga tctgcgccgt ggcgatgccg
    15121 caggcattgc atttgacgcc gatccaactc accgcagtgc cggtgaaggt gaacgtggcg
    15181 gtggcggtcg cctggttgga cgcccggatc gtgccgccgc tgaaggtgcc agtttcgggg
    15241 ccgtagggtg gccagtagcc tgtgtaggtg gctgtgtctt cctcgaagcg ggtaacggtc
    15301 gggccatcga tctggaattt cgccacaaaa ccgtcgtcat ctccgccgga aaccggctgc
    15361 agggccccga cagtcgcagg gaaattaccg gagttggtcc agccggtaat gtaggttcca
    15421 ccggaagtat cgacggcaat tcccaatccg ctgtcggcac cactgccgcc gatgtaggtg
    15481 gaatacacga gagccgaacc cgttggatcg agcgtcgaca cgaaggcatc gctggcagaa
    15541 ccgccatggt ctggctggaa ggctcccggc gtagtcggaa agccggtcac gcacgacgag
    15601 aatggtccac agaccatata agcgtcgcct gtcacgtgga cgcggccgac agcgtcgatg
    15661 gcaattcccc tgcccacggc gccgccaggc ccgccgagga gggtggagta gacgaatgcc
    15721 gaaccggcgg cattgagctt cgccacatat gcgtttgtgc tgccgccgga catggtctgg
    15781 aaggcgccgg gcgtcgtggg aaagtcggaa ccgtcggtgg cgcctgtaac gtaggcgttg
    15841 ccggaggcgt cgacggcaac ggcggcggcg ctccccttca agtaggtgga gtagacgagc
    15901 gccgaccctg cgggctccaa ctttgtaaca aagccgccgc cttgggttga aacaggctgt
    15961 tgggcgccgg gagtggtggg gaaatccgtt gaaggcgtag atccaactac gtatgcattc
    16021 ccgctggcgt ccaccgcaat cccttgaccc cagtcgtcgt caccgctgcc gccgagatag
    16081 gtggaataca cgagggctga gccagcggaa ttgagcttcg tcacgaaggc gtcgccgctg
    16141 aaatccgcgc ccgcgtggtg tggctggaag gcgagtgcgg tggtcgggaa gtcgatggag
    16201 tctgtgttgc cggtgatgta cgcgcttccg gccgcatcca ccgcgatcgc gtaggcctga
    16261 tcgagattgt cgccaccgat gaacgtagag taagcgaggg tggagccggt tggtccgagc
    16321 ttcgtgacga aagcatcggt catggacctg tgtgtctgtt ggtacgcacc cgcagtcacg
    16381 ggaaagtcat gcgagtcggt cgaaccggtg acgtaggcat tgccgccggc atccacagcg
    16441 atcgcactgc cgttgtcgcg caagctcccg ccgaggtagg tcgaatagac aagcgcggtg
    16501 ccagtggaat tgagcttcga gacgaaggcg tctcccgtgg agaaaggtcc gccggttcca
    16561 ccgtaaatca tttggtgggc gccaatggtg gtcggaaaat caacggaacc ggtttccccg
    16621 acgacgtagg cattgcccgc cgcgtcaacg gcaatcccga caccgaggtc gcgctggctc
    16681 ccaccgagat acgttgcgta ggcgagcacc ggatcgatga ccagcggctc gcccctgtcg
    16741 taagcggcga cctggaatcc gacccgatgt gcatctttga tcacatagcc gccttcgatc
    16801 tcctgccgaa cgccctcgac ttcctggtaa atgacgggct tgtgctggcg cagctcgccg
    16861 ctggcggtgt gcagcacgag gtttccctgg gcgtcgattt tgatcgcagt ggcgccctcg
    16921 aaacgcagca gaatcttttc cggatccgcc cccggcgcaa cgacgaagtc gtactccagc
    16981 tgtcgctggt tgccgtagta caccagatcg attccgtcgt agacctcgcg gtagtggacc
    17041 ttggcgtaag tcggcacgtt ggtgcgccac ttcgcgggat ccttgccgat gaagtaattc
    17101 gccttgccgg ggagttcctc gagtcctctc gccagcgcat ccgcgttggc gccgaccagc
    17161 gccatgcgca cgaccgtccg gtccttggct cccaacgcga gcaccgcttc gccagcggtg
    17221 agatacaggc cgtatccggg gccgcgcgcg aggaagcgca cgtccttgtg cgcctgcccc
    17281 cggttctctt cgaaatgcag cggtagccga ccgtaggtct cggtcaggcc cgcaccggca
    17341 accgccggca gggcgaggac gataaggaaa ctgaacagcg cgaaggcagt tcgaagcagg
    17401 gctgacaaca tggcgatctc ctatgccaag ttttcctacc ctttatgcca aacgcttatg
    17461 acgtgatcgg agtgtgtggg cgctcggcgc gtagtccatc cggccccggc gcatagtccg
    17521 tcttgcgcgt ccatccccgc cgccaggcat cgcgcagggg cagatacacc ctgccgcgga
    17581 gactggccgg cgccgtgcat tcatacacgc gcagctgctc gccgccgaag tcgcgcttga
    17641 agcggttgat gccttcgcgc tccggggtgg attcgtcctc gaatagcccg ccccagtcgt
    17701 agcgctcaaa tcccatggcc ttgagcgcca gcaggtcttt ccagtgcagc cagcggtttg
    17761 cgcgcccgac gagcgcgcgc acgtcgctct cctggccgcg aaaatgcgag gcggtgtact
    17821 gcagtccggc ggtctggccg cacagcacgt gcgcgtgcca gacgagggga cctgcgtcat
    17881 tcgttgcgca ggagagcgcc aactgctccg caacgcacgc ggcttcgagc cagtggcgat
    17941 cgcacgggct gtgccccttc tgcttcgcga aggcgtcgaa gaactttgca aactcctcca
    18001 atcggctctc cggcgcagcg ctgaactcca ggcgcagccc gtctttggtg tcggcgcggc
    18061 ggaccttgta gcggcagtcc ttgccgaagt gcgcggcgat cgcctcttcg tcctcggtga
    18121 ggtcggtgac catggtgagg agtggcgcga tacgcgcctt ggccaccggc tcgctcctga
    18181 accggtactg gacgatgtcc acggtagcat ccggcgaagg ctcctcgccg aaccagactt
    18241 ccccgtaggt ggccgcgcgg ccgcggagca ggatcatgtc aggcgctcgc cgcggcgtgg
    18301 aattctcgca gcgtcatgcc gcggcaccag acgcgcaaat tggtgaacag gagctcgagg
    18361 cgctcgtaga gcttctcgat gtcgtccgcg ctgcggaacc tcggactacc gccaggcatg
    18421 agttccgacg aatgcagcat gaactccagg tgggctgcgc cttcgtcgcg cgcgatgcgt
    18481 gcgacttgca gcatctcatc gaggccgctt tccgccggac acagccagtt caggctgcgc
    18541 gagagccggt tggcggctcc gcgcaggagc ggcacccgat agatccaggg cgcaatgcgg
    18601 tacagagttc cggagcgaac cgtcacgggc acctccatca gcgggcccgc ggctggcacc
    18661 gagatatcgc gcgggtcgac gaagtagggg cgttgcggaa agccccggta gtcggctccg
    18721 ccgccgccgc gggggtcgcc cagattgggg ctccagtcga cgcccggcgt caccgagcaa
    18781 tcgacgcgat atccctcgtc gacgagcatc gcggcgtagc ggccatccat cgcccagcgc
    18841 ccggcgcgat ggctcacgac attgcgcccg aagcgattct cgagcaggcg cgtcatgaag
    18901 tggatttttt cccgcatcac ctcgtcgggg tactcgatga ggaatggctg gtgatggaaa
    18961 tcgtccccgg tgagcgagaa cagtggcggc gagttccatg cgtgcagatg catccccact
    19021 tcgcctgcgc tgcgcgcgag cacgtcgcgc gcgaagtcga cgaactcccc gttcatcgcc
    19081 atttcgtaat tggtgaggta caccggcttg aaaccgaagc gctcgcacag cgcttggaaa
    19141 cggggcaggt gagccgcgtt gcgcgtcgtg atctcgcgcg gcctcgccca caggtcgtcg
    19201 ccctcggtgt cgatcgtgac gatgaagggc atcttggaca cgaaaagagc atagcttcgt
    19261 cgcatgaagg cttgccgggc gcaggcggcc aagccctagt ccgtttggac cattcgcttg
    19321 gatcgtgtgc ggtttgcttg tcacgcccga gcttgcaaca ccagcaaaaa cgtacccggg
    19381 ccgaacatag atgtcgctct aaatggacgg tccgatcggc gcaagcgaac ttggcaatcg
    19441 cgtaaccgga tcccggtata cgtgtggtcg gatgcactac tgagccatag aggagggacc
    19501 atgtacacgg tgcgcgcgtc tgcaattgct cttcttttcc ttagtgctgc gggcaacgtc
    19561 gtagcgcagg cgcgggaccc ggttgtttgg cctttcgcga gtaccagcat gtggaacatg
    19621 cccataggga gcaacgcgaa ttatgtggcg ataaacatgc caattccgga ggaccccggc
    19681 tttcccccga gcattccacc caccaactgg gcgccgatgc ccggcataca tgacgaatac
    19741 atcgtcatgg cgcctacggc gcccatgaca gacatctatt acagtaacgt cggttggtcg
    19801 ggaggaaatc ggtgcgcgcc tacgggaatc ccgccgatat tgctgaccca ggtgcccgtt
    19861 ccgacgaact atgtcgtgga caatggcggg ggagacaatt cagcggcatt tctgatgccg
    19921 gatggcagga ccctcatcca gtctcaaccc ttcacccgct gccttgccgg tgggccggca
    19981 acaagcatca tccacttcga ctacgtcgac ctttacggag atggccgatg gggagctcac
    20041 ggcgggtcgc agctctccag tgttggtggg tccattcacg taggagagct tcgtccgggc
    20101 atacctgttc gacacgcact cgcaatgacg ctaaatacga ctgcggtcct tgcaccttgc
    20161 gatgcgatgg acgattgcta tcgttggccg gcgtattccg gcgacgctgg tgctacgaac
    20221 ccaacaaccg gttatggaag gatgaatccg aatccggttt cggggatgaa gatgggagcg
    20281 ctgcttgcca tcccggcaaa cgtcgacgtc gaaaacggtc tgggactgga atcggggccc
    20341 gggaagatgc tcgcctggac gctgcagaac tatggggttt acatcgttga cagttcgggc
    20401 ggcccttcga tcttcattga tgccgacacc gggcccaatg gctcatttgc ggggtcgaag
    20461 cgagaagagt tcttcaatga tttcggcatg cccttcgagc agagagtcaa ctctgatacc
    20521 ccttggtctc gcgacatgca gaaattgata caggcactgc acctagtaga caacaacggt
    20581 cccagcagca tcgggggtgg cggcacgcca cttcagcctc tcgctccggc tttggcagtc
    20641 acgcgcatcg aggaagacaa ctccacagta accttcagcc ccagtggtgg ctgggtggaa
    20701 cggcaaaggg aagtggctgg cttcaacgac gggcgagccc tgagctacga tctgagcgga
    20761 ggtaccgcga cgttcagctt caccggcacg ggcgtgagtt ggatcgggct caaatgcaat
    20821 gtttgcggga tcgcgagcgt gtcaatcgat ggcggagcgc cgaccatggt gaacacggct
    20881 ggtcctgcgg gtcctggcag ctttgggcta cggtcggagc ccgtctttac ggcatctggg
    20941 ctagtagccg gtccccacac attgcagatc gccgtcaccg ggaccaccag ttcgggcggc
    21001 tcgcacatcg ccgtcgatgc attcgatgtg acgccctgat ttcggctaat ccggccgct
//
LOCUS       DASRIE010000098         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3499592, whole genome shotgun sequence.
ACCESSION   DASRIE010000098 DASRIE010000000
VERSION     DASRIE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499592"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFB53_04425"
     CDS             complement(<1..677)
                     /locus_tag="VFB53_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86015.1"
                     /translation="MFTAVVRITGAGRLDDFRERVRWLMVRDEDAEDYTERFEGGALE
                     YRFELRRGIPFPAFTAASADFPELRVEAEWERDGERGRAVIENGQLVEHRAAPLGEVK
                     VDVQVGDDARLELGLTCRQGGAGGLVGYAVTDERHTYWRYRDGALSLLELEDPDAELE
                     DVAFRFVDEWIWYDEEEAALERVRYANYGYPVRGANVKSEKLALLRKNDGRYSSMGDE
                     GKALREA"
     gene            complement(684..905)
                     /locus_tag="VFB53_04430"
     CDS             complement(684..905)
                     /locus_tag="VFB53_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZN86016.1"
                     /translation="MAKKASDTVYRVTEVIGTSADSWEDAARNAVKTAAGSLRDLRIA
                     EIEKLDVKIEDGRITQFRARLALSFKYEG"
     gene            complement(951..3224)
                     /locus_tag="VFB53_04435"
     CDS             complement(951..3224)
                     /locus_tag="VFB53_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZN86017.1"
                     /translation="MTLAALAFAAGAAVLQLQPVLPPLAAAAVLLLAVLRCRVLWVPL
                     ALAAGFFWAAALAHLRMAGWLAPELEGRDLDVVGVVSSLPAVSERAVRFEFEVESARE
                     RLPAKILLAWHRGPASEEGPALLAGGVHPGERWLFTVRLRRPHGHVNPNGFDYEAWLL
                     ERGVGATGYVRQRGTQERLAPRNSWMDRVEQTREAVRDRFQERLGSTPAAGILAALAV
                     GDQRAISADEWRLFNRTGVTHLMSISGLHVTLVSGLAAWLVSFGWRRVPAAVLRLPAR
                     KAAAMAAIAAALGYTLLAGFAVPAQRTFYMVAVVAAALWSGRIASPARTLALALAVVV
                     AADPWAPLSPGLWLSFGAVALIFYVAANEGRLAQWARMQWAITLGLAPAALLLFGQVS
                     LAGPLANAAAIPLVSIVVTPLALLAVVLPFDWILECAAWLVEWLLGFLEWCAALPGAL
                     WQQHVPPAWAAAAAICGAAWILAPRGVPWRAAGAALMAPAFALAPAAPAAGEAWVTTL
                     DVGQGLAVVVRTASRALLYDAGPSYGPEADSGGRIVVPMLRGAGIESVDLMVVSHEDS
                     DHLGGALTLLETLEVRALATSLPRQHALHALAAAPARCAAGRAWEWDGVRFEFLHPPP
                     GWEGARRNNQSCVLRVEAGGASMLLTGDIERAAESVLSEALRRTDVLLVPHHGSRTSS
                     SEAFIAAAAPRWAIVSAGYRNRFGHPAREVLERYAGAGIVLLRTDMHGAINVVLKAGQ
                     ARVEGERALRPRYWRRV"
     gene            3261..3812
                     /locus_tag="VFB53_04440"
     CDS             3261..3812
                     /locus_tag="VFB53_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZN86018.1"
                     /translation="MPRKLFRKYLPDPESLRSSRMVGVFGRWLHHPNLWHLNRRSVAG
                     AVAIGLFSGLVPGPLQMLTALLLAVPLKKNIPVALIVTLYTNPLTIVPLYLLAYAYGQ
                     LLLPGERAAHVAPFHFDLGHFAESMRGLGEWAMSLGRPLVVGLVALACTLALLGYVAV
                     QVGWRAYVVAAWRARARRRKGPA"
     gene            3809..4159
                     /locus_tag="VFB53_04445"
     CDS             3809..4159
                     /locus_tag="VFB53_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86019.1"
                     /translation="MTHEEFVAAYQAGRVRVRIDRKAASRFVAGRAMLPLVLLPLLGL
                     GVALALVGYILSGTIVFLAALLLRLLVRRSSNGFLLWRALRDGEFYDQVVAAQVLKVE
                     MPDAEGVSTPSRSS"
     gene            complement(4096..4800)
                     /locus_tag="VFB53_04450"
     CDS             complement(4096..4800)
                     /locus_tag="VFB53_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN86020.1"
                     /translation="MSEAVVSCRDLRKTYQGPAPVEVLQGVNLEVRAGERIAIVGRSG
                     SGKSTLLHVLGGLDSPTSGSVRVQGRPFSAMSESERGRVRNATLGFVYQFHHLLPEFT
                     AVENVAMPLWIRRSDRNAAYERAKKVLTEIGLGARLEHQPGELSGGERQRCAFARALV
                     TEPACVLADEPTGNLDSHTADEVFDAMVRLSASHGTAFVIVTHDSDLAARAQRIYELR
                     DGVLTPSASGISTFST"
     gene            complement(4793..6037)
                     /locus_tag="VFB53_04455"
     CDS             complement(4793..6037)
                     /locus_tag="VFB53_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZN86021.1"
                     /translation="MRPYELLVGLRYTRARRRNRFIGINSAVSMIGIAVGVWALIVVL
                     SVMNGFQKEVRTRILGVVSHLQVLADDNRLEDWQGVQKAVAQHPRVVGTAPFVQAQAM
                     LSNGQAVRGAVVRGILPAEEEKVADLARHMRAGSLEDLRPGEFGVVLGADLARSLGVL
                     RGDKIALVAPQGVVTPAGVIPRLKQFSVVGVFEAGIVDADSGLALVHMRDAQTLYQLG
                     EAVSGVRLKLDDLFAARAVGRELLARLPRDVFAYDWTRSHANFFRAVEIEKRMMFLIL
                     GLIVLVAAINIISTLVMAVTDKQADIAILRTLGAAPGSVLQIFMVQGMVLGVVGTLIG
                     VVTGIVTALNIDVIVPAIEHAFNIKFLSKDVYLIPDLPSDLQANDVVWIAGMALALSF
                     LATIYPSWRAGRVNPAEALRYE"
BASE COUNT          929 a         2255 c         2110 g          830 t
ORIGIN      
        1 atcgcttcac ggagggcttt gccttcatcg cccatggagg aatagcggcc gtcgttcttg
       61 cgcagcagcg cgagcttctc ggacttcacg ttcgcgccgc gcaccgggta gccgtagttc
      121 gcgtagcgca cgcgctcgag cgcggcctcc tcctcgtcgt accagatcca ttcgtcgacg
      181 aagcggaacg ccacgtcctc gagctccgcg tcggggtcct cgagctcgag cagcgagagc
      241 gcgccgtcgc ggtagcgcca gtaggtatgg cgctcgtccg tgaccgcgta gccgacgagg
      301 ccgccggcgc cgccctggcg gcaggtgagg ccgagctcga gccgcgcgtc gtcgcccacc
      361 tggacgtcga ccttaacctc gcccagcggc gcggcgcggt gctcgacgag ctgtccgttc
      421 tcgatcaccg cgcgcccgcg ctcgccgtcg cgctcccatt cggcctcgac gcgcagctcg
      481 ggaaaatcgg ccgacgccgc ggtgaatgcc gggaacggaa tgccacggcg caactcgaag
      541 cggtattcga gcgcgccgcc ttcgaagcgc tcggtgtagt cctcggcgtc ctcgtcgcgc
      601 accatcagcc agcgcacgcg ctcgcggaag tcgtcgagcc tgccggcgcc ggtgatgcgc
      661 acgaccgcgg tgaacacgcc cgctcagccc tcgtacttga acgacagcgc gagacgcgcg
      721 cggaactgcg tgatgcggcc gtcctcgatc ttcacgtcga gcttctcgat ttcggcgatg
      781 cgcaggtcgc gcaggctgcc ggccgcggtc ttgaccgcgt tgcgcgcggc gtcctcccag
      841 gaatcggcgc tggtgccgat gacttcggtg acgcgataaa cggtgtcgct cgccttcttt
      901 gccatggtcg atcctcctcg aaggtcgcgg gagagccccg cccgaagatt ctatacgcgc
      961 cgccagtagc gcgggcgcag cgcgcgctcg ccctcgaccc gcgcctggcc tgccttcaac
     1021 acgacgttga tcgcgccgtg catgtcggtg cgcagcagca cgatccccgc ccccgcgtag
     1081 cgctcgagca cctcgcgcgc gggatggccg aagcggttgc ggtagcccgc ggacacgatc
     1141 gcccagcgcg gcgccgcggc ggcgatgaat gcttcgctcg acgaagtccg gctgccgtga
     1201 tgcggcacca gcaatacatc ggtcctgcgc agcgcctcgg acaggaccga ctcggcggcg
     1261 cgctcgatgt cgccggtcag gagcatggag gcgccgccgg cttccacgcg cagcacgcag
     1321 ctctggttgt tgcgccgcgc gccctcccag ccgggcggcg gatgcaggaa ctcgaagcgc
     1381 acgccgtccc attcccacgc ccgccccgcg gcacagcgcg ccggcgccgc cgcgagcgcg
     1441 tgcagcgcat gctggcgggg aagcgaggtg gcgagcgcgc gcacctccag cgtctcgagc
     1501 agcgtgaggg cgccgccgag gtggtcgctg tcctcgtggc tcaccaccat gaggtccacg
     1561 ctctcgatgc ctgcgccgcg cagcatcggc acgacgatgc ggccgccgct gtcggcttcg
     1621 ggcccgtagc tgggcccggc gtcgtagagg agcgcgcggc tcgcggtgcg caccacgacc
     1681 gcgagcccct gtcccacgtc gagcgtcgtg acccaggcct cgcccgcggc cggcgcagcc
     1741 ggcgcgagcg cgaacgccgg cgccatcagc gcggctcccg ccgcgcgcca tggcacgccg
     1801 cgcggcgcga ggatccacgc cgcgccgcag atcgcggccg cggcggccca cgcgggcggg
     1861 acgtgctgct gccagagcgc gcccggaagc gccgcgcacc actcgaggaa gccgagcagc
     1921 cactcgacga gccaggccgc gcactccagg atccagtcga acggcagcac caccgcaagc
     1981 agggcgagcg gcgtcaccac gatcgatacg agcggaatcg cggccgcgtt cgcgagcggc
     2041 ccggcaaggg aaacctggcc gaaaagaagc agcgcggccg gggcgaggcc gagcgtgatc
     2101 gcccactgca tgcgcgccca ctgtgcgagc cgcccttcgt tcgccgcgac gtagaagata
     2161 agcgccaccg cgccgaacga gagccacagg cccggcgaga gcggcgccca aggatcggcg
     2221 gcgaccacga ccgcgagcgc aagcgcgagc gtgcgcgccg gcgaggcgat gcggcccgac
     2281 cagagcgccg ccgcgaccac cgccaccatg tagaaggtgc gctgcgccgg caccgcgaac
     2341 cctgcgagca gcgtgtagcc gagcgcggcc gcaatcgccg ccatggccgc cgccttgcgc
     2401 gccggcaatc gcagcaccgc cgcaggcaca cgccgccagc cgaacgagac cagccacgcc
     2461 gcgagcccgg aaaccagcgt gacgtgcagc cccgagatgc tcatgaggtg ggtgaccccg
     2521 gtgcggttga acagccgcca ctcatcggcc gagatcgcgc gctggtcgcc gaccgcgagc
     2581 gcggccagga tcccggccgc gggcgtcgag ccgagccgct cctggaaccg gtcacggacg
     2641 gcttcgcggg tttgctcaac cctatccatc catgaatttc ttggggcgag ccgttcctgc
     2701 gtgccgcgct ggcgcacata gccggtcgcg cccacgccgc gctcgagaag ccacgcctcg
     2761 tagtcgaagc cgttcggatt gacgtgaccg tgcggccggc gcagccgcac ggtgaagagc
     2821 cagcgctcgc ccggatgcac gccgcctgcg aggagcgccg ggccctcctc gctcgcgggg
     2881 ccgcgatgcc aggcgagcag gatcttcgcc ggcaggcgct cgcgcgcgga ctccacctcg
     2941 aactcgaagc gcaccgcgcg ctcgctcacc gccggcaggc tggagaccac gccgaccacg
     3001 tccaggtcgc gcccttcgag ctccggcgcg agccagccgg ccatgcgcag gtgcgccagc
     3061 gcggcggccc agaagaagcc ggcggcaagg gcgagcggca cccagagaac tctgcatcgc
     3121 agaaccgcga gcagcaacac cgccgccgct gccagcggcg gcagcaccgg ctgcagctgg
     3181 agtaccgccg cgcccgccgc gaacgccagc gccgccaagg tcatctagaa tgcaacgcgc
     3241 cgacccgctc ccggctgaca gtgccgcgca agcttttccg caagtacctt cccgatcccg
     3301 agtcgctccg ctcgagccgc atggtgggcg tgttcggccg ctggctgcac catccgaacc
     3361 tgtggcacct gaaccgccgc tcggtggcgg gcgcggtcgc gatcggcctg ttctccgggc
     3421 tggtgcccgg gccgctgcag atgctcacgg cgctgctgct cgcggtcccg ctgaagaaga
     3481 acatcccggt cgcgctgatc gtcacgctct ataccaatcc cctgacgatc gtgccgctct
     3541 acctgctggc atatgcctac ggtcaactgc tgctgcccgg cgagcgagcg gcgcacgtgg
     3601 cgccgttcca tttcgacctg gggcacttcg ccgaatcgat gcgcgggctg ggcgagtggg
     3661 cgatgtcgct cggccggccg ctcgtggtcg gcctggtggc gctcgcatgc acgctcgcgc
     3721 tgctcggcta cgtggcggtg caggtcggct ggcgtgctta cgtggtcgct gcctggcgcg
     3781 cgcgcgcgcg gcgccgcaag ggccccgcgt gacgcacgag gaattcgtcg ccgcctacca
     3841 ggcggggcgc gtgcgcgtgc gcatcgaccg caaggcggcc tcgcgcttcg tcgcgggacg
     3901 cgccatgctg ccgctggtcc tgctgcccct gctggggctc ggcgtcgcgc tcgcgctggt
     3961 cggctacatc ctcagcggca cgatcgtgtt cctcgccgcg ctgctcctgc gcctgctcgt
     4021 gcggcgctcc agcaacggct tcctgctatg gcgcgcgctg cgcgacggcg agttctatga
     4081 ccaagtggtc gctgctcagg tgctgaaggt cgaaatgccc gacgcagaag gtgtgagcac
     4141 gccgtcgcgc agctcgtaga tgcgctgcgc gcgggcggcg aggtcgctgt cgtgcgtgac
     4201 gatgacgaac gcggtgccgt gcgaggccga gagccgcacc atcgcgtcga atacctcgtc
     4261 ggcggtgtgc gagtcgaggt tgccggtggg ctcgtcggcc agcacgcacg ccggctcggt
     4321 caccagcgcg cgcgcgaagg cgcaacgctg gcgctcgccg ccggagagct cgcccggctg
     4381 gtgctcgagg cgcgcgccga ggccgatttc ggtgaggact ttcttggccc tctcataggc
     4441 agcattcctg tccgaacgac gaatccagag cggcattgcg acattctcca ccgccgtaaa
     4501 ctccggcagc aggtggtgga actggtagac gaagccgagg gtggcgttgc gcacgcggcc
     4561 gcgctcggac tcgctcatcg cgctgaaagg cctgccctgg acccgcacgc tgcccgaagt
     4621 cggcgagtcc agcccgccga gcacgtgcag cagcgtcgac ttgcccgagc cggagcggcc
     4681 gacgatcgcg atgcgctcgc ccgcgcgcac ctcgaggttc acgccctgca gcacctcgac
     4741 cggcgccggg ccctggtaag tttttcgcag atcccggcac gacacgaccg cctcactcat
     4801 accgcagcgc ctccgcggga ttaacgcgcc ccgcgcgcca gctcggatag atcgtggcga
     4861 ggaacgacag cgccagcgcc atgcccgcga tccagaccac gtcgttcgcc tgcaggtcgg
     4921 acggcaggtc cgggatgagg tacacgtcct tcgagagaaa cttgatgttg aaggcgtgct
     4981 cgatcgccgg cacgatcacg tcgatgttga gcgcggtcac gatgccggtg accacgccga
     5041 tcagcgtgcc gacgacgccg agcaccatgc cctgcaccat gaagatctgc agcaccgagc
     5101 cgggcgcggc gccgagcgtg cgcaggatcg cgatgtcggc ctgcttgtcg gtcaccgcca
     5161 tcaccagcgt cgagatgatg ttgatcgcgg ccaccagcac gatcaggccg aggatcagga
     5221 acatcatgcg cttctcgatc tcgaccgcgc ggaagaagtt ggcgtggctg cgcgtccagt
     5281 cgtaggcgaa cacgtcgcgc ggcagcctgg cgaggagctc gcgccccacc gcgcgggcgg
     5341 cgaaaagatc gtcgagcttg aggcgcacgc cgctcaccgc ctctcccagc tggtagaggg
     5401 tctgcgcgtc gcgcatgtgc acgagcgcga gacccgagtc ggcgtcgacg atcccggcct
     5461 cgaatacgcc caccacgctg aactgcttga gccgcgggat cacgccggcc ggcgtcacca
     5521 cgccctgcgg cgcgaccagc gcgatcttgt ccccgcgcag cacgccgagc gagcgcgcga
     5581 ggtcggcgcc cagcaccacg ccgaactcgc cgggccgcag gtcctcgagc gagccggcgc
     5641 gcatgtggcg cgcgaggtcc gccaccttct cctcctccgc cggcaggatg ccgcgcacca
     5701 ccgcgccgcg cacggcctgg ccgttggaaa gcatcgcctg cgcctgcacg aagggcgccg
     5761 tcccgaccac gcgcggatgc tgcgccaccg ccttctgcac gccctgccag tcctccagcc
     5821 ggttgtcgtc ggcgagcacc tgcaggtgcg acaccacgcc gaggatgcgc gtgcgcactt
     5881 ccttctggaa gccgttcatc accgacagca ccacgatcag cgcccacacg ccgaccgcga
     5941 tgccgatcat ggaaaccgcg gaattaatgc cgatgaagcg attgcgccgc ctggcgcggg
     6001 tgtagcgcag gccgacgagc agctcgtagg gacgcaaatt gggccagggc ggcaggcaaa
     6061 ggcgcgagtg tgccacaacc gcaggccggc gggcgcgcgt ggcattgcaa gtcggtcgca
     6121 gaga
//
LOCUS       DASRIE010000099         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1420707, whole genome shotgun sequence.
ACCESSION   DASRIE010000099 DASRIE010000000
VERSION     DASRIE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420707"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFB53_04460"
     CDS             complement(<1..149)
                     /locus_tag="VFB53_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZN86022.1"
                     /translation="MLSAMLTDETRYRILKLLEADPHASQRRISDELGISLGRVNYCL
                     NALIN"
     gene            287..1141
                     /locus_tag="VFB53_04465"
     CDS             287..1141
                     /locus_tag="VFB53_04465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN86023.1"
                     /translation="MKFLPRRAPGAFPQVSVIIGVKNGAATLQRCLESIAAQEFADRE
                     TIVVDSASTDGTRELLEEARRQGKVTDYVSEPDGGLYEAWNKGVLRSRGDWICFLGCD
                     DAFHDTRALRHLAGARAERGIVYGLVNRVTPRGAVAEVWGGPWTEWRAPSFAGFMIPH
                     PGSLHHRSIFEERGMFDESYRVAGDYELVLRELLVREPVFVDRVVVDMRFGGMSSRPA
                     AIYRTLQEVQRARAAHGLHGKPARLRQALAAAWIGSWIQRLLGDWTYRWCADLYRLVR
                     GKPRIWTA"
     gene            1149..2276
                     /locus_tag="VFB53_04470"
     CDS             1149..2276
                     /locus_tag="VFB53_04470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86024.1"
                     /translation="MIRVLTFGRFADDNFGGLERYVFELGRALEGEVEFVNIVARRGA
                     PPDVRLAGETVYAAPLATLGGTPICPSMPWHALRRHRRAPFDIAHLQFPADPMAHLAY
                     ALLPRGVGRVIGWHSDIVRQQGLLALYRPFLERSLRGAHAIIAATPAHVSSSEQLAPV
                     RDTAQFRIIPYGFDLARFERRPAQADELRQRFGGRFLVFALGRHVYYKGFEFLIRALA
                     QLPGVELALGGQGPLTGELREIARQAGVADRVHFVGRIPDRDLPAWYYACDAFCLPSV
                     EAAEAFGIVQVEAMACGRPVVCCELGNGVNWVNQDGETGLTVPPADPAALAAALARLL
                     AEPALRARLGEAGSRRAHGVFSARAMAQATLAVYREVLSRL"
     gene            complement(2249..3679)
                     /locus_tag="VFB53_04475"
     CDS             complement(2249..3679)
                     /locus_tag="VFB53_04475"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HZN86025.1"
                     /translation="MASSIVPVILCGGSGSRLWPMSRKLLPKQFLPLVTQQTLFQDTA
                     LRTRGVPGIAAPIVVTNAEQRFLAADQLAEIGVASRALLLEPVGRNTAPAVAVAAVRA
                     LQDDADAVLLVQPSDHAIGDVAAFQAAVGRAVAAARGGHLATFGVPPAGPATGYGYIE
                     AGEAISPEVYRIRRFIEKPTAEKARALLAQGGFYWNSGMFVFLARHYLEELGRLRPEI
                     LAAARAAIGKARADLAFLRLDDAAFAASPSDSIDYAVMERTAAGAVVRASMRWSDVGS
                     WTSLWEAGDKDAAGNVARGDVALRDTAGCYVHADGRHVSVLGARDLVVVETDDAVLVA
                     AKDRAQDVKEVVEQLQREKRSEHVSHSRVHRPWGYYEGVDAGPGFQVKRLMVKPGHRL
                     SLQLHRQRAEHWVVVAGTARVTRGDEVFDLQCNQSTYIPVGTKHRLENATGEPLLIVE
                     VQSGAYLGEDDIVRFEDNYNRDSTSR"
     gene            3732..4505
                     /locus_tag="VFB53_04480"
     CDS             3732..4505
                     /locus_tag="VFB53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN86026.1"
                     /translation="MRILVTGAGGFIGSHLCPALAGAGHVLQGAVEGSDAVVHLANIA
                     HTAASPAELHRVNVEGTIARAQEALGAGARRFVYLSSIKAAHPEDAYGRSKSLAEQAL
                     CGLPGLEPVILRPALVYGPRVKANFLALLKAIDAGWPLPLASIENRRSLVYVGNLVDA
                     ILRCLDAPVPGQVFAVSDGAPVSTPELCRAIARALGRPARLFPFPGALLRLAPPIARL
                     VQSQVADDAAIRAELGWRAPFSFEEGLRRTAEWYRARGG"
     gene            4516..>5169
                     /locus_tag="VFB53_04485"
     CDS             4516..>5169
                     /locus_tag="VFB53_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86027.1"
                     /translation="MLALAPVAAFLVALLAARLLLTAAGRRIALDTPNERSLHDRAVP
                     RTGGIAIAAGVAAGVALVQPGLAVTLIAALALAVVFFVDDVASLPTLVRFVAQLGAAA
                     AVVVYQLGYAEPVLFTVFVLAIAWYANLYNFMDGSDGLAGGMAAIGFGAYAAAAQVSG
                     AGPFAALCASIAAAAGAIRSAANRATSSWNEDCSAVVSRIMFLLLNHLPGRVVSSHHA
                     "
BASE COUNT          699 a         1804 c         1834 g          832 t
ORIGIN      
        1 ttgttgatca gcgcgttgag gcagtagttg acccggccca ggctgatgcc gagctcgtcg
       61 gagatgcgcc gctgacttgc gtgcgggtcg gcttcgagca gcttgaggat cctgtagcgg
      121 gtctcgtccg tcagcatggc cgatagcatc tcatatatgt tcagatcatg aacatacaca
      181 cactaacata ttgatattgc atgtgtttac ttatgtgaag caagttcatt tgatgaacat
      241 gccggccccg gccggtcccg tataagttga taccatcggc acccggatga aattccttcc
      301 ccgtcgcgcg cccggcgctt ttccccaggt cagcgtcatc atcggcgtga agaacggcgc
      361 cgccacgctg caacgctgtc tcgaaagcat tgctgcgcag gaatttgcag accgcgaaac
      421 gatcgtcgtg gacagcgcgt cgaccgacgg tacgcgcgag ttgctcgagg aggcccggcg
      481 ccagggcaag gtcacagact acgtctccga gcctgatggc gggctgtacg aggcttggaa
      541 caagggtgtc ctgcgcagcc gcggcgactg gatctgcttt ctcggctgcg acgacgcgtt
      601 ccacgacacg cgggcgctgc gccacctggc cggcgcccgc gccgagcgcg ggatcgttta
      661 cgggctggtc aaccgggtta cgccgcgggg cgcggtggcc gaagtctggg gcggtccctg
      721 gaccgagtgg cgtgcgcctt ccttcgccgg attcatgatt ccccatcccg gcagcctgca
      781 ccaccgctcc atcttcgagg agcgggggat gttcgacgag tcctaccgcg tcgccgggga
      841 ctacgagctc gtcctgcggg agctgctggt gcgcgagccg gtgttcgtcg accgggtggt
      901 ggtcgacatg cgcttcggcg ggatgagctc gcgcccggcc gcgatctacc gcaccctgca
      961 ggaagtgcag cgcgcgcgcg cggcgcacgg cctgcacggc aagcccgcgc ggttgcgcca
     1021 ggcgctggcg gcggcgtgga tcggctcgtg gatccagcgg ctgctgggcg actggaccta
     1081 tcgctggtgc gccgacctgt accggttggt gcgcggcaag cccaggatct ggaccgccta
     1141 ggcgcaccgt gatccgcgtc cttaccttcg gccgcttcgc ggacgacaac ttcggcgggc
     1201 tggagcgcta cgttttcgag ctcggccgcg ccctggaagg cgaggtggaa ttcgtcaaca
     1261 tcgtcgcgcg gcgcggcgcg cccccggacg tgcgcctggc gggggaaacg gtgtacgcgg
     1321 cgccgctggc gacgctcggc ggcacgccga tctgcccctc gatgccctgg catgcgctgc
     1381 gccggcaccg gcgcgcgccg ttcgacatcg cgcacctgca gtttcccgcc gatcccatgg
     1441 cgcacctcgc ctatgcgctc ctgccgcgcg gggtggggcg ggtgatcggc tggcacagcg
     1501 acatcgtgcg ccagcagggg ctgctcgcgt tgtaccggcc gttcctggag cgcagcctgc
     1561 gcggagcgca cgccatcatc gccgcgacgc cggcgcacgt cagctcgtcg gagcagcttg
     1621 cgccagtgcg cgatacggcg cagttccgca tcattcccta cggtttcgac cttgcgcgct
     1681 tcgaacggcg gcccgcgcag gcggacgagc tgcgccagcg gttcggcggg cggttcctgg
     1741 tgttcgccct gggccgccac gtctattaca agggcttcga attcctgatc cgcgcgctgg
     1801 cgcaattgcc cggggtcgaa ctcgcgctgg gcgggcaggg cccgctgacc ggcgagcttc
     1861 gggagatcgc gcgccaggcc ggcgtagcgg accgagtcca tttcgtcggc cgcatcccgg
     1921 accgggacct tcccgcctgg tactacgcct gcgatgcgtt ctgcctgcct tcggtggagg
     1981 cggccgaagc cttcggcatc gtgcaggtgg aagcgatggc ctgcggccgg ccggtggtgt
     2041 gctgcgagct cggcaacggc gtcaattggg tgaaccagga tggcgaaacg gggttgacgg
     2101 tgccgcccgc agacccggcg gcgctcgctg ccgcgctggc gcgcctgctg gcggagccgg
     2161 cgctgcgcgc ccgcctgggt gaggctggaa gccgccgcgc gcacggagta ttcagcgcgc
     2221 gggccatggc gcaggcgacc ctggcggtct accgcgaggt gctgtcccgg ttgtaattgt
     2281 cctcgaagcg cacgatgtcg tcctcgccga ggtaggcgcc ggactgcact tcgacgatca
     2341 ggagcggttc gcccgtagcg ttctccaggc ggtgcttggt gccgaccggg atgtaggtcg
     2401 actggttgca ctgcaggtcg aagacctcat cgccgcgggt gacgcgggcg gtgccggcaa
     2461 cgaccaccca gtgctcggcg cgctggcggt gcagttgcag cgacagccgg tggcccggct
     2521 tcaccatcag ccgtttcacc tggaagcccg gcccggcgtc cacgccctcg taatagcccc
     2581 aggggcgatg cacgcgcgag tgcgagacgt gctcgctgcg cttctcgcgc tgcagctgct
     2641 ccaccacctc cttgacgtcc tgcgcgcggt ccttcgccgc caccagcacg gcgtcgtcgg
     2701 tttcgacgac caccaggtcg cgggcgccga ggaccgacac gtggcggcca tccgcgtgca
     2761 cgtagcagcc ggcggtgtcg cgcagcgcca cgtcgccgcg cgcgacattg cccgcagcgt
     2821 ccttgtcgcc ggcctcccag agcgaagtcc aggagccgac gtcgctccag cgcatcgagg
     2881 cgcgtaccac ggcgcccgcc gcagtgcgct ccatcacggc gtagtcgatc gaatcggagg
     2941 gcgacgccgc gaacgcggcg tcgtccaggc gcaggaacgc aaggtcggcc ctggctttgc
     3001 cgatggcagc ccgcgccgcg gcgagaattt ccgggcgcag ccggccgagc tcttccaggt
     3061 agtggcgcgc caggaacacg aacatgccgc tgttccagta aaagccgcct tgggcgagca
     3121 gggcccgcgc cttctccgcc gtgggcttct cgatgaagcg ccggatgcgg tagacctccg
     3181 gcgagatcgc ctcgcccgcc tcgatgtagc cgtagccggt ggcggggccg gcgggcggca
     3241 cgccgaaggt cgccaggtgc ccgccgcgcg ccgctgccac cgcgcggccc accgcggcct
     3301 ggaacgccgc cacgtcgccg atcgcgtgat cggacggctg cacgagaagg accgcgtcgg
     3361 cgtcgtcctg caaggcccgc acggcggcca ccgcgaccgc gggcgcggtg ttgcggccga
     3421 cgggctccag cagcagcgcg cgcgaggcga cgccgatctc ggccagctgg tccgccgcga
     3481 ggaagcgctg ctcggcattc gtaaccacga tcggcgcggc gatgccgggc acgccgcgcg
     3541 tgcgcaacgc ggtgtcctgg aacagcgtct gctgcgtgac cagcggcagg aactgcttgg
     3601 gcagcagttt gcgcgacatc ggccacaggc ggctgccgga gccgccgcac aggatcaccg
     3661 gaacgatgga actggccaag ggaatgcccg cgtggagaac ggccgctatt ctaagggcga
     3721 taatcacccc aatgcgcatc ctggtcaccg gcgccggcgg cttcatcggc agccaccttt
     3781 gccccgcgct cgcgggcgcc ggccacgtgc tgcagggcgc ggtcgagggc agcgacgcgg
     3841 tggtgcacct cgccaacatt gcgcacaccg cggcgagccc cgcggaactg catcgagtca
     3901 acgtcgaggg cacgatcgcg cgtgcgcagg aggcgctcgg tgcgggcgcg cgccgcttcg
     3961 tgtatctcag ttcgatcaag gcggcgcatc ccgaagacgc ctacgggcgc agcaagtcgc
     4021 tcgccgagca ggcgctgtgc ggcctcccgg gcctggagcc ggtgatcctg cgcccggcgc
     4081 tggtctacgg cccacgcgtg aaggcgaatt tcctcgcgct gctcaaggcg atcgacgccg
     4141 gctggccgct gccgctcgcc agcatcgaaa accggcgcag cctggtgtac gttggcaact
     4201 tggtcgacgc gatcctgcgc tgcctcgacg cgccggtgcc gggccaggtg ttcgccgtaa
     4261 gcgacggcgc acccgtatcc acgcccgaac tgtgccgcgc catcgcgcgc gcgctcggcc
     4321 ggccggcgcg cctgttcccg ttcccgggcg cgctgctgcg cctcgcgccg cccatcgcgc
     4381 ggcttgtgca atcgcaggtc gccgacgacg cggcgatccg cgccgagctc ggctggcgcg
     4441 cgccgttcag cttcgaagaa ggcctgcgcc gcaccgcgga gtggtatcgc gcgaggggcg
     4501 gctagaatgc gcgccatgct cgcgctggcg ccagtggccg cgttccttgt cgcgttgctg
     4561 gccgcgcgtc tgctcctgac ggcggcgggc cggcgcatcg cgctcgacac gccgaacgag
     4621 cgctccctgc atgaccgggc ggtgccgcgt accggcggca tcgccatcgc cgccggcgtc
     4681 gccgccggcg tggcgctcgt ccagcccggc ttggccgtga cgctaatcgc cgcgctggcg
     4741 ctggcggtgg tgttcttcgt cgacgacgtc gccagcctgc cgacgctggt gcgcttcgtt
     4801 gcgcagctcg gtgcggccgc ggcggtggtg gtgtaccagc tcggctacgc cgagccggtg
     4861 ctgttcacgg tgttcgtcct cgccatcgcc tggtacgcca acctgtacaa cttcatggat
     4921 gggtcggacg gcctcgccgg cggcatggcg gcgatcggat tcggcgccta cgcggcggcg
     4981 gcgcaggtgt ccggcgcggg ccccttcgcg gcgctctgcg cgtcgatcgc cgcggccgcc
     5041 ggcgcgatcc gctcggcggc aaatcgcgcc acctcttcct ggaatgagga ctgttccgcg
     5101 gtcgtttcga gaatcatgtt cctcctattg aaccatttac cgggccgggt cgtctcatcg
     5161 catcatgca
//
LOCUS       DASRIE010000100         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_113437, whole genome shotgun sequence.
ACCESSION   DASRIE010000100 DASRIE010000000
VERSION     DASRIE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113437"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1680
                     /locus_tag="VFB53_04490"
     CDS             28..1680
                     /locus_tag="VFB53_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZN86028.1"
                     /translation="MRELFSRFRPFFAYAGLFSLAINVLLLVPPLYMLQVFDRVLASR
                     SGETLAMLTIAAVVALLVMALLDVLRARLLATAGAALDRRLGPRVLDGLLSQTARLTG
                     AGYLNGLRDVNTLRSFLGGAGLITLFDAPWLPFFLLVIFLFHPVLGAVALAGALAMVL
                     LAFLNERLTRQPIERAQAQARRAARFIDANVRNAEVVSALGMLPAVTRRWCGLNDSAL
                     GEQARAASVGGLFLGATKFTRQFIQLAMLAAGAYLVVAQDVTAGVMIAATILLGRALA
                     PVETLVAGWRGMVEARGAWRRLAELLKNSAPAEPTTELPVPRGLLEVERAVFGFKEKP
                     ILRGVSCRLEPGEALGLIGPSASGKSTLARLIVGVWKPLAGAVRLDGADVAAWPRERL
                     GPHIGYLPQDVELFGGTVAENIARLGEPDDAQVVRAAQRAHVHELILRLPKGYDTEIG
                     EAGQALSPGQRQRIALARALYGNPRLVVLDEPNANLDHEGEEALVRTLQILKADGATV
                     VIIAHRPWLLRGVDKMLVLREGLVERFGASSEIMARFARQAA"
     gene            1677..>2539
                     /locus_tag="VFB53_04495"
     CDS             1677..>2539
                     /locus_tag="VFB53_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZN86029.1"
                     /translation="MNELEDIRGPVRIGLLLIASVIIGFAAWALFAPLSGAVIAPGYV
                     KVDLNRKIVQHQEGGIVRAIRVRDGDRVTQGQELVLLDDVRIDAQLDLLRTQLDAERA
                     KAARLEAERTMAARPVFPQDLQKKEIVVREEALFRARREALDSQIAVLKRQIRETVEE
                     AGALAGQIAAEERALKLQKEELSANERLLAQGYVQKTRLLTLQRGVAEYEARHGERRA
                     ELSKSRQRASELEFRILAMRNTYVQTATDELKESTSKMFDLEERIRPSRDASERQKIA
                     APIAGEVVGLR"
BASE COUNT          357 a          879 c          923 g          380 t
ORIGIN      
        1 catcgtggtg gtttgaaata ggagcgcgtg cgcgagctct tttcccggtt taggccattc
       61 ttcgcgtacg cagggctgtt cagcctcgcg atcaacgtgc tgctgctcgt gccgccgttg
      121 tacatgctgc aggtgttcga ccgcgtgctc gcgagccgca gcggcgagac gctggccatg
      181 ctcacgatcg ccgcggtcgt cgcgttgctg gtgatggcgc tgctcgacgt gctgcgcgcg
      241 cgcctgctcg ccacggccgg cgcagcgctc gaccggcgcc tcggcccgcg cgtgctggac
      301 ggcctgctct cgcagactgc ccggctcacc ggcgccgggt acctgaacgg cctgcgcgac
      361 gtgaacacgc tgcgcagctt cctcggcggg gcggggctca tcacgctgtt cgacgcgccg
      421 tggctgccgt tcttcctgct ggtcatcttt ctcttccatc cggtgcttgg cgcggtggcg
      481 cttgccggcg ccctcgccat ggtgctgctc gcgttcctga acgagcgcct gacgcgccaa
      541 ccgatcgagc gcgcacaggc gcaggcgcgg cgcgcggcgc gtttcatcga cgccaacgtg
      601 cgcaatgcag aagtcgtcag cgcgctcggc atgctgcccg ccgtcacgcg gcgctggtgc
      661 gggctcaacg attccgccct gggcgagcag gcgcgcgccg cgagcgtggg cggcctcttc
      721 ttgggcgcca cgaagttcac gcgccagttc atccagctcg cgatgctggc ggcaggcgcg
      781 tacctcgtcg tggcgcagga cgtgaccgcg ggggtgatga tcgcggccac catcctcctc
      841 ggccgcgcgc tcgcgccggt ggaaacgctg gtcgccggct ggcgcggcat ggtggaagcg
      901 cgcggcgcct ggcggcggct cgcggagctg ctgaagaact cggcgccggc cgagcccacg
      961 accgagctgc ccgttccgag gggactgctc gaggtggagc gcgcggtctt cggcttcaag
     1021 gagaagccca tcctgcgcgg cgtgtcgtgc cggctcgagc ccggcgaggc gctcgggctg
     1081 atcgggccga gcgcgtcggg caagtccact ctcgcgcgcc ttatcgtcgg cgtctggaag
     1141 ccgctcgcgg gcgcggtgcg cctcgacggc gcagacgtag ccgcgtggcc gcgcgagcgg
     1201 ctcggccccc acatcggcta cctgccgcag gacgtcgagc tcttcggcgg cacggtcgcg
     1261 gagaacatcg cgcgcctcgg cgagcccgac gacgcacaag ttgtgcgcgc agcgcaacgg
     1321 gcgcacgtgc acgagctgat cctgcgcctg cccaagggct acgacaccga gatcggcgag
     1381 gccggccagg cgctctcgcc gggccagcgc cagcgcatcg cgctcgcgcg ggccctctat
     1441 ggcaatccgc gactcgtggt gctggacgag cccaacgcca acctcgacca cgagggcgag
     1501 gaggccctgg tgcgcacgct gcagatcctc aaggccgacg gagcgaccgt cgtgatcatc
     1561 gcccaccggc cttggcttct caggggcgtg gacaagatgc tggtgctgcg cgaggggctg
     1621 gtggagcgct tcggcgcaag ctcggaaatc atggcgcgct tcgcgcgcca ggccgcatga
     1681 acgagctcga agacatcagg ggaccggtgc gcatcgggct gctgctgatc gctagcgtga
     1741 tcattggctt cgcggcctgg gcgttattcg cgccgctttc cggcgcggtg atcgcgccgg
     1801 gctacgtcaa ggtcgacctc aaccgcaaga tcgtgcagca ccaggagggc ggcatcgtgc
     1861 gcgccatccg ggtgcgcgac ggggatcgcg tcacacaggg gcaggagctg gtgctgctcg
     1921 acgacgtgcg catcgatgcg cagctcgacc tgctgcgcac ccagctcgac gccgagcgcg
     1981 ccaaggccgc gcggctcgag gccgagcgca ccatggccgc ccgcccggtg ttcccgcagg
     2041 acctgcagaa gaaggagatc gtggtgcggg aggaggcgct gttccgcgcc cggcgcgagg
     2101 cgctcgacag ccagatcgcg gtgctcaagc gccagatccg cgagacagtg gaggaggccg
     2161 gcgcgctcgc cgggcagatc gccgccgagg agcgcgcgct caagctgcag aaggaggagc
     2221 tttcggcgaa cgagcggctc ctcgcccagg gctacgtgca gaagacgcgg ctgctcacgc
     2281 tgcagcgcgg ggtcgcggag tacgaggcgc gccatggcga gcgccgcgcc gagctttcca
     2341 agtcgcgcca gcgcgcctcg gagctcgagt tccgcatcct cgcgatgcgc aacacctacg
     2401 tgcagacggc gaccgacgag ctcaaggaat ccacttccaa gatgttcgac ctcgaggagc
     2461 ggatccggcc ttcgcgcgac gcctccgagc gccagaagat cgcggcgccc atcgcggggg
     2521 aagtggtggg cctgcgcat
//
LOCUS       DASRIE010000101         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1421806, whole genome shotgun sequence.
ACCESSION   DASRIE010000101 DASRIE010000000
VERSION     DASRIE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421806"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFB53_04500"
     CDS             complement(<1..609)
                     /locus_tag="VFB53_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZN86030.1"
                     /translation="MLPIPNKILTPLIVAAGALLIAAAPPQALAPRLSAAVVCTPEDM
                     AWGPEPAAEPLDATQQALVEHLSRRFFIASAATERMVAAAHRAALEVGLDPLLVLAVI
                     SVESRFNPIAESVMGAKGLMQIIPKYHLDKLRAAGGEDAVFDPESNIHVGARILQEYV
                     HRTGTLEAGLQFYNGALRDNTAQYAQKVLAERDRLEQVLRSRN"
     gene            824..1801
                     /locus_tag="VFB53_04505"
     CDS             824..1801
                     /locus_tag="VFB53_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZN86031.1"
                     /translation="MNIVALVLAGGEGTRLYPLTAEHAKPAVPFASGYRLVDFVLSNL
                     VNSRISTIYVLAQYKPDSLIKHISAAWAPWFADGDGIIKVLLPRSNTLSGQFKGTADA
                     VYQYLELVQAHSPDLVAVFAADHVYRMDVRQMVAFHQERRADVTVSAVGVPLKQASNF
                     GILATGADGRVKDFREKPKQAAPLPCDPSRAYASMGNYLFEPEVLEQALLDARRAGET
                     DFGRDVLPRLCRTHRVFAYDFAENRVPGVQEHEEPAYWRDVGTLAALAAAQQDAISTP
                     PRFNLWNRRWPIRGEFDAALLARIRSVNAPAPWQASSRDLRSDERLPAS"
     gene            complement(1731..>3132)
                     /gene="glgA"
                     /locus_tag="VFB53_04510"
     CDS             complement(1731..>3132)
                     /gene="glgA"
                     /locus_tag="VFB53_04510"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZN86032.1"
                     /translation="GFSQVGSSATMRVLFVTPECAPLAKAGGLGDVSAALPAALRALG
                     HEVDLLLPGYSEVLGKLPDAAETERFSELGFECRLLRSGGLLLIDCAPLYRREGSPYQ
                     DPGGSDWPDNALRFGLLSKVAARLAPRYDVVHCNDWPAALAPAYAHPEKTLLTIHNLA
                     FQGNFDPSALGRLGLPDALFTMQGLEFHGRVSFLKGGLVYAGALNTVSPSYAREIQTE
                     ELGCGLDGVLRGRRDALSGILNGIDLEVWDPARDAHLAQRYDERSLDKKSVNKAALQR
                     RLNLEPSDDALIGFVGRLTHQKGADLIAAAAAELAALPAQLAVLGRGERELEGVLAAA
                     AARHPGRIALAIGFNEELAHLIEAGADLFVMPSRFEPCGLNQMYSQRYGTPPVVRATG
                     GLADTVADNETGFLFERAESSALAAAVRRALAARRDPGRWREIQRAGMRRDFSWRAAA
                     RRYADLYSRLATAPGR"
BASE COUNT          468 a         1110 c         1081 g          473 t
ORIGIN      
        1 gttgcggctg cgcagcacct gctcgagccg gtcgcgctcg gcgagcacct tttgcgcgta
       61 ttgcgccgtg ttgtcgcgca gcgcgccgtt gtagaactgc agccccgcct ccagggtgcc
      121 ggtgcggtgc acgtactcct gcaggatgcg ggcgccgacg tggatgttgc tttccgggtc
      181 gaaaaccgcg tcctcgccgc cggcggcgcg cagcttgtcg aggtggtact tcgggatgat
      241 ctgcatcagg cccttcgcgc ccatcacgct ctcggcgatc gggttgaagc gcgactcgac
      301 cgagatcacc gccagcacca gcagcgggtc gagcccgact tccagcgcgg cccggtgcgc
      361 cgcggccacc atgcgctcgg tagccgccga ggcgatgaag aagcgccgcg agaggtgctc
      421 gacgagcgcc tgctgggtgg cgtcgagcgg ctcggcggcc ggctccggcc cccaggccat
      481 gtcttcgggc gtgcagacca ccgccgcgga aagccgcggc gcgagcgcct gcggcggcgc
      541 ggcggcgatc agcagcgctc cggcggcgac gatcagcggt gtcaggatct tgttgggaat
      601 cgggagcatc ggcgcacccc gcgggtagga gccgaatgct aggcgggccg cgcggcgcgc
      661 gatatcgccg ggagcttaat ttcctgtcgc gcggcgacta cagcgcgctc ttatgcgcgc
      721 tacatgcgga tacgagtccg ggcgatagtg ctgttcgcta ccgctcaata tcatttcggc
      781 gtcgcgtcga aacaaatttc gttacaggaa aggcgaaccc agtatgaata tcgtggcgct
      841 ggttcttgca ggcggggaag gcacgcggct ttatccgctc accgccgagc atgcgaagcc
      901 cgccgtgccg ttcgccagcg gctaccggct ggtggacttc gtactgagca acctggtgaa
      961 ttcgcgcatc tcgacgatct acgttctcgc gcagtacaag cccgactcgc tgatcaagca
     1021 catctcggcg gcatgggcgc cgtggttcgc cgacggcgac ggcatcatca aggtgctgct
     1081 gccgcgctcg aacacgctga gcggccagtt caagggcacg gccgacgccg tgtaccagta
     1141 cctcgagctg gtgcaggcgc attcgcccga cctggtggcg gtcttcgccg ccgaccatgt
     1201 ctaccgcatg gacgtgcggc aaatggtggc tttccaccag gagcgccgcg ccgacgtcac
     1261 ggtttccgcg gtcggcgtgc cgctcaagca ggcctccaac ttcggcatcc tcgcgaccgg
     1321 cgccgacggc cgggtcaagg acttccgcga gaagcccaag caggccgcgc ccctgccctg
     1381 cgatccgtcg cgcgcctacg cctcgatggg caactatctg ttcgagcccg aggtcctgga
     1441 gcaggcgctg ctcgacgcgc gccgcgcggg cgagaccgat ttcgggcgcg acgtgctgcc
     1501 gcgcctgtgc cgcacgcatc gcgtgttcgc ctacgacttc gccgagaacc gcgtgcccgg
     1561 cgtgcaggag cacgaggagc ccgcctactg gcgcgatgtc ggcacgctcg ccgcgctcgc
     1621 cgccgcgcag caggacgcga tcagcacccc gccgcgcttc aacctctgga accggcgctg
     1681 gccgatccgc ggtgagttcg acgccgcgct gctcgccagg atccgctcgg tcaacgcccc
     1741 ggcgccgtgg caagcctcga gtagagatct gcgtagcgac gagcggctgc ccgccagctg
     1801 aagtcgcgcc gcatcccggc gcgctggatc tcccgccagc gcccgggatc gcgccgcgcg
     1861 gcgagcgcac ggcgcaccgc cgccgccagc gcgctcgatt cggcacgctc gaagagaaac
     1921 ccggtctcgt tgtccgccac cgtatcggcg agcccgccgg tcgcgcgcac gaccggcggc
     1981 gtgccgtagc gctggctgta catctggttc aggccgcacg gctcgaagcg cgagggcatc
     2041 acgaacaggt cggcgccggc ctcgatcagg tgagcgagct cctcgttgaa gccgatcgcc
     2101 agggcgatgc gccccgggtg ccgcgccgcg gccgcggcga gcacgccctc cagctcgcgc
     2161 tcgccgcgcc cgagcaccgc gagctgcgcc ggcagcgcgg cgagctcggc agccgccgcg
     2221 gcaattaggt cggcgccttt ctggtgcgtg aggcgcccga cgaagccgat cagcgcgtcg
     2281 tcgcttggct cgaggttgag ccgcctctgc agcgccgcct tgttgaccga tttcttgtcc
     2341 agggagcgct cgtcgtagcg ctgcgctagg tgcgcgtcgc gcgccggatc ccagacttcg
     2401 aggtcgatcc cgttcaggat cccgctcagc gcgtcgcgcc gtccacgcag caccccgtcc
     2461 aggccgcagc cgagctcctc ggtctggatc tcccgcgcat aggagggact caccgtgttc
     2521 agagcgccgg cgtagaccag gccgcccttc agaaacgaga cgcggccgtg gaactcgagc
     2581 ccctgcatgg tgaagagcgc gtcgggcagg ccgaggcggc cgagcgccga cggatcaaaa
     2641 ttaccctgga acgcgaggtt gtggatcgtg aggagcgtct tctccggatg cgcgtacgcc
     2701 ggcgcaagcg cggcaggcca gtcgttgcag tgcacgacgt cgtagcgcgg cgccaggcgg
     2761 gcggccacct tggagagcag cccgaagcgc agcgcattgt cgggccagtc gctgccgccg
     2821 gggtcctgat acgggctgcc ctcgcgccgg tagagcggtg cgcagtctat gaggagcagc
     2881 ccgccgctcc tcagcagccg gcattcgaag cccagctcgc tgaagcgctc cgtctcggcc
     2941 gcatccggca gcttgccgag gacctcagag tagcccggca gcagcaggtc cacttcgtgc
     3001 ccgagcgcgc gcagcgcggc gggtagcgca gcgctgacgt cccccagccc gccggctttc
     3061 gcgagcggcg cgcactcagg cgtaacgaac agcactcgca tggtcgcgct ggaacctacc
     3121 tgagaaaaac cc
//
LOCUS       DASRIE010000102        21557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_888503, whole genome shotgun sequence.
ACCESSION   DASRIE010000102 DASRIE010000000
VERSION     DASRIE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888503"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFB53_04515"
     CDS             <1..470
                     /locus_tag="VFB53_04515"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HZN86033.1"
                     /translation="ALEPQVGEGLRSNVRAVFEAIAHRNPYPQAWFDTHRWNHMVLKA
                     LFIGSPLAPIQGLDARANAELARIMRDFAHERWAAGRPVPFEIWRCVGPFAEGPALED
                     LARVLRKGEAIEKRAAALALAASPDREAARLLQTVPGLAGDISLKRLSWATLQ"
     gene            522..1421
                     /locus_tag="VFB53_04520"
     CDS             522..1421
                     /locus_tag="VFB53_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZN86034.1"
                     /translation="MISRTTDDYEAMVASGVVAVIEPAFWIGQPRTNVGSYLDYLSSI
                     VGFERFRASQFGMRHYCTIGLNSKEANNQELAEGVMELIPRFALKEGVVAIGEIGYDE
                     QTPLEDKYFRLQLELAKKLDLPVMIHTPHRDKKRGTTRSMDVCDEHGLDPAKVVVDHN
                     NEETVRETLERGYWAAFSIYPSTKMGNARMVEILRQYGAKQVIVDSACDWGISEPLGV
                     AKTAKLALQQGIPESDVRLACYANALRAYGQSGQMKEEDWLNPQAIDQRAVFEGNSIL
                     RGGREPRVEERKGRRTMDDLIIE"
     gene            1586..2887
                     /locus_tag="VFB53_04525"
     CDS             1586..2887
                     /locus_tag="VFB53_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN86035.1"
                     /translation="MNFARTGAALSAATMIAFSAGAQAQVKIRLEPLVTGINTPLAMV
                     QPAGDARMFIIEQNGRIKILEGGKLRPTPFLDIRSKIPALHHDFDERGLLGLAFHPNF
                     KSNGKFYVSYSAHLDYGADLGQMLWYSHSNVVEEYTVSKGDKNTADPSSARRIMSTAW
                     PQFNHNGQWIGFGPDGKLYVSMGDGGYANDWGIGHNPAIGNGQDMSTVLGKILRIDVD
                     ARTGDKAYGIPSDNPFTGDKNAKPEIWALGLRNPWRCSFNTSGGELFCGDVQQNSYEA
                     IKIVGKGQNMGWRRVEGIMRCFDYLKPDDHPANCDKAGITPAIIEYNNCTAKPQGCKG
                     ISITGGYVYQGKEAGWKGKYIFGDWSKSFGEMDGQIFFGTKGSDGKWSMEVAEVTNRK
                     GKLPYVLAFAQDRNGEVYALTSITTGPNGSLDTIYRIVPSK"
     gene            2970..3326
                     /locus_tag="VFB53_04530"
     CDS             2970..3326
                     /locus_tag="VFB53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN86036.1"
                     /translation="MKVRTACALLGLLAAVAALAQEHKAVKEPLPAFDKAYLSNRKNV
                     AAGKDVWDNQCRHCHGAAAYPGKAPKLNPGQLAPDFIYERVTYGFGKMPAWKDVFSLE
                     QRKSVVAYIKSRDFSP"
     gene            complement(3334..3690)
                     /locus_tag="VFB53_04535"
     CDS             complement(3334..3690)
                     /locus_tag="VFB53_04535"
                     /inference="COORDINATES: protein motif:HMM:NF026420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HZN86037.1"
                     /translation="MRYLLVLPGVVMVAFAAVQYNDPDALVWIAIYLVPAAWAFAAAF
                     RPAQVCSLAGERLLWASVAIGAALTVFYWPTMPNFWVKDVWWVEETAREGMGMMIAFA
                     VLLAVLATTLLRKRAA"
     gene            3720..4916
                     /locus_tag="VFB53_04540"
     CDS             3720..4916
                     /locus_tag="VFB53_04540"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZN86038.1"
                     /translation="MTTDAQGAQARTDIHWQRFSVPYEFPVVFTEGVLDPENPALRDV
                     LCRLEPGKRHRAVFFYDDGLNAGRAPLAEAIQNYAQRHAEAIELACAPISLPGGEKIK
                     SELHFVESIQQTLFDLHLDRHSYVVAIGGGAVLDAVGLVAATTHRGVRHVRIPTTVLA
                     QNDSGVGVKNGVNLQGVKNFVGTFAPPFAVLNDLAFVMSLPERDKIAGMAEAVKVALI
                     RDAEFFGWLERHMDDLATFERRAMAHMIRRCAELHMRQIGQGGDPFETGSARPLDYGH
                     WSAHKLESLTRHHVRHGEAVAIGMALDARYSVLAGLLAAGQEERICALLEYLGFTLWH
                     PALAKAGAGGEWAILEGLKDFQEHLGGELTVTLLGGIGTGVEVHEIDVARMREAMHWL
                     KDRAGA"
     gene            4916..6112
                     /gene="eboE"
                     /locus_tag="VFB53_04545"
     CDS             4916..6112
                     /gene="eboE"
                     /locus_tag="VFB53_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HZN86039.1"
                     /translation="MHLAASAAGRDLGHLTYCTNIHAGEALQEVQAGLARYLPEIKAG
                     VSPGRPFGVGLRLGHAAAEGLRDRAALEALKRFLAEGGYYVFTLNAFPYGAFHGRAVK
                     EDAYRPDWSEPERLAYTDHAADLLSELLPAGAEGSVSTVPCTFKPWAAGRMEAIVEHL
                     IRHVAHLVALRGRTGQTIALALEPEPYCYLETIEETVAFFEAHLFHRAGVARLAALTG
                     LGPGAAEAAMRRHIGVCYDVCHAAVEFEDARASVARLRAAGIAIGKLQLSSALRVAAL
                     DAQSAQHLAAFAEPVYLHQAVERSGGRLRRYLDLPEALGAAARGAELRVHFHVPVFLE
                     QMQNFGTTQAFLAEILALHRAEPISRHLEVETYTWDVLPASYRGAGLGPAIARELNWV
                     KAQLLQ"
     gene            6109..6957
                     /locus_tag="VFB53_04550"
     CDS             6109..6957
                     /locus_tag="VFB53_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZN86040.1"
                     /translation="MKLGTALRLGRVSNLPTVWTNTLAGAVLAGAGAYGARFALMLLA
                     FSLFYTGGMFLNDAFDARIDAVQRPERPIPSGDIGARAVFAWGYGMMALGTVMLAWFG
                     VASALAGLTLAVAITFYDWHHKQNVFGPVVMGLCRVLVYVGAGLCITLALPDRLWLGA
                     ALIWCYLVGLTYVAKQENLDRVENLWPLLFLAAPVAGGAWLAAAQPAAGVFWLLFTGW
                     MLVALWFLRRRAKGDVPRAVVSLIAGIALLDALLIAAAGAPALAALALAGFAVTWFLQ
                     RYIAGT"
     gene            7057..8262
                     /locus_tag="VFB53_04555"
     CDS             7057..8262
                     /locus_tag="VFB53_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN86041.1"
                     /translation="MNTKTTLTLAFTAASLIAAGAQAQTVKIGVVLPYTGVGAEFGQQ
                     VDRGIQMFLKLNPTAFGGHKVELVKRDSKNPSGAEAKTAVQELIAQDKVDLLTGFIYS
                     PDAIASAPLVSAAKKPMVVMNAGTAWITNLSPMISRVSFSMWHSGHAMGEAAAKLLKA
                     KTAVVGYSDFPPGKDSLDAFMRGFEAHGGKVVDAIPMGGPGQVPDFTPFFQRAKDRKP
                     DVFFVFVPAGDHAVSVVKTYAALGMRQAGIKLIGPGDITQDFKLQAMGDDAVGLITMH
                     HYNADLDNAANRRLVAAWRQEYGANAVPDFMGVQGYDGMAAIAHAVTALKGSMDPDKV
                     QAALKGWKYADSPRGPISIDPATRDIVMNEYLSELVKKDGRLYQKNIGVITAVKDMCK
                     ELKQGKCAN"
     gene            8283..9146
                     /locus_tag="VFB53_04560"
     CDS             8283..9146
                     /locus_tag="VFB53_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86042.1"
                     /translation="MDLASILLGGLAAGMVLFVVSVGLSVTMGLMGFVNLAHGGFAML
                     GGYAVVLAMRHWGLPFSAALAFGFAAIAALSVLLERTLYARLYRASELDQVLFTIGLV
                     FVMIASVTLAVGPESQRVELPAWLDGQTDLGFLRYRSYSLALIVAGTLLMLALWLGFE
                     RTRTGAKIRAAVDNRRMAESLGVDVGRLFTLTFALGSGLAAVGGGLGAEILGLDPQYP
                     LRYLAYFLIVVAVGGLGRVTGVFCAALLIGVLDFTLKIYFPKGGTMFIYMLTLGFLLA
                     RPQGLFGKRGT"
     gene            9143..10123
                     /locus_tag="VFB53_04565"
     CDS             9143..10123
                     /locus_tag="VFB53_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86043.1"
                     /translation="MSSAAMHELAMRHRLQWWEALPWAAGLAAFFLFPNYLAFGTQVL
                     VTILFALSLDLVLGYAGIVTLGHAAFFGAGAYAVGMLAAHAGWNEPLSGLVAAALVAG
                     LLGLVSGWILLRTHGLTLLMLTLCTMALLEQAANMFSGWTGGFDGMPGLSFTPILGLF
                     EFDVLYFRTQYLYALAALLLGFAVVRVLVYSPFGRSLTGIRENLLRMHAVGAPVRARQ
                     VLVYAISAALAGVAGAVFVQATGYVNLAVLGLERAAGVLIILILGGYGRLYGAFVGAL
                     VYMLLEHTLSKAYPTAWQLGLGLTLMLVALFARNGIFGLGDALRRRWSAR"
     gene            10150..10875
                     /locus_tag="VFB53_04570"
     CDS             10150..10875
                     /locus_tag="VFB53_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86044.1"
                     /translation="MCKSFGALEVARRIDFRLQAGARHALIGPNGAGKTTFVNLLTGV
                     LAPSAGRVLLDGEDVTRLAQARRVKRGIARTFQINQLFRGLTVLENVCIAVAERMRAG
                     GGMLRPAGQRAEVVAEAARLIALLQLDADAGRRVGELPYGRQRLVEIAIALGLQPRVL
                     LLDEPAAGIPSAESHILLEAIGALPADIAVLIIEHDMDLVFRFAERITVMVGGAIFAE
                     GRPAEIAANREVRAVYLGGALDG"
     gene            10868..11569
                     /locus_tag="VFB53_04575"
     CDS             10868..11569
                     /locus_tag="VFB53_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86045.1"
                     /translation="MAEALRLSGVSAGYGETHVLEGIELALAAGESLALIGRNGVGKS
                     TLLATLMGHTTLHAGSIELAGARIEAAPPHRRSLAGLAWVPQEREIFPSLTVRENLEL
                     GARPGHWDMARVCGLFPNLADRLEHGGTQLSGGEQQMLSIARALLTNPSVLLMDEPTE
                     GLAPVIVQALGQVLTTLREAGGLAIVLVEQNSRVALEFSERTVVMDKGRIAYDGAAER
                     LRRDPEFLASLIAAQ"
     gene            11585..12097
                     /locus_tag="VFB53_04580"
     CDS             11585..12097
                     /locus_tag="VFB53_04580"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HZN86046.1"
                     /translation="MALSAFNFARWIDEHAHLLRPPVGNQQVFAEAGDLVVMVVGGPN
                     ARTDYHDDPYEEFFYQLRGNMVLKVIEDGRPRDIPIREGDILLIPGHLRHSPQRPEAG
                     SVGLVVEKVRPRDVNDAFEWYCPQCWARVHRVEVNVQDIVKDLPPLFEGFYRSARRCP
                     QCGHVHPGKG"
     gene            12094..13092
                     /locus_tag="VFB53_04585"
     CDS             12094..13092
                     /locus_tag="VFB53_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN86047.1"
                     /translation="MNPRAIDMHSHFFPREWEDLGRRFGTPDWPSMKHLGDGRAMVML
                     GEREFRPVTSACWDAGRRLEEMDRDGVDLQVMCSTPVLFAYARPAAQALHCAQLFNDA
                     GLALAARGKGRLKTLCQVPLQDTDAACKELSRAMRAGHLGVQIGNHVGNRDLDDEGIV
                     AFLQHCAAEGAAVLVHPWDMMGGARTSRWMMGWTVSMPAETQLSIVSLILGGGFDRLP
                     KDLRLCFAHGGGSFAFLLGRLENAWHNRDVARGLSEHPPSHYLDRFSVDSAVFDPRAL
                     RLLIEVMGEDRVMLGSDYPFPLGEQRIGKLVRDMEDLGAGARRKLLAGNAKRFLGL"
     gene            complement(13089..13319)
                     /locus_tag="VFB53_04590"
     CDS             complement(13089..13319)
                     /locus_tag="VFB53_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(13287..13289),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HZN86048.1"
                     /translation="MQVVITYCVPUNYLPRAAGLAAQIKQALGIEAELAKGKGGVFDV
                     ACDGKLVFSKHRDGRYPETEEIIRALAALKGA"
     gene            13348..14343
                     /locus_tag="VFB53_04595"
     CDS             13348..14343
                     /locus_tag="VFB53_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HZN86049.1"
                     /translation="MKITVLDDYFDTVRMLECFAKLAGHEVTVWNDHLQETGGLAARL
                     ADTDALVLIRERTQIRAPLLERLPRLRLISQRSVYPHIDIDACTRLGVVVSSSQHAGT
                     PSYAAAELTWGLVLAAERSIPQQMASLRAGRWQMGVGNSLRGRTLGIHGYGRIGSAVA
                     GYGRAFGMKVMVWSRPDSLAKARADGYATASSKEEFFANCDVITLHLRLIEATRGIVT
                     AADLARMKPDSLLVNTSRAGLIAPGALVAALKAGRPGRAAVDVFETEPLRDTADPLLA
                     MDNVIATPHIGYVTREEYELQFTDIFDQILAYAAGRPMNVVNPAALAARSAGTRA"
     gene            complement(14297..15298)
                     /locus_tag="VFB53_04600"
     CDS             complement(14297..15298)
                     /locus_tag="VFB53_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN86050.1"
                     /translation="MIIDCHGHYTTAPASLWEWRKLQAAGRDPGPLRISDDEIRDSLE
                     KNQLRIQRERGTDLTFFSPRAGSMEHHVGDARMSEDWSRQCNDLIHRVCSLYPSRFAP
                     VCQLPQSPGVPPANCIPELERCVKELGFIGCNLNPDPSGGWWKSPPLGDRFWYPLYDK
                     LVELEVPAMVHVSSSCNPAFHFTGAHYLNADTTAFMQFLQSDLFKDFPTLKFVIPHGG
                     GAVPYHWGRYRGLAQDMGKPGLKDLLLGNVFFDTCVYHLPGIELLLKVIPAENILFAS
                     EIVGAVRGVDPETGHYYDDTRRYIDAVGNLSAADREKIYSGNVRRVYPRFGRRAPRG"
     gene            complement(15295..16101)
                     /locus_tag="VFB53_04605"
     CDS             complement(15295..16101)
                     /locus_tag="VFB53_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN86051.1"
                     /translation="MKALLGDYPCTAQFKRTAPLAFEEVRPPSAGFKRVVRNLEFDFA
                     ELAITTFLMAKAAGKPYRLLPAVVLARMQHPRLVCDRTRPVAPRSLEGKRIGVRSYSV
                     TTGMWIRGILEEDYAVDHRRITWVTFEEAHVAEFRDPANVERAPAGRKLEEMLLAGEV
                     DAAVSGDAPATDPRIVPLIPDAEGAARAWRARTGAIQINHMVAVKDSVSRADADGILR
                     RLEESFAAAGNPELNPIGLQANRRNLEVAIDYVHRQQLIPRRYAVEELFA"
     gene            complement(16098..16970)
                     /locus_tag="VFB53_04610"
     CDS             complement(16098..16970)
                     /locus_tag="VFB53_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HZN86052.1"
                     /translation="MSERIAFIGFGEAGQTISRGLAGSSKPAIRAYDILFGQPAGAPL
                     EAAAKPLGVALARDHADAVRESDLVMLTVTAASSLEAARSCLPGLRKDQLFLDMNSVS
                     PRRKIETAALVAPTGAAYVDVAVMAPAAPYLHKVPCLIGGPGAAALAPRAAALGMQME
                     FVSAEVGQASAIKMFRSIVVKGLEALLVESMTAAAEYGVEDRVLASLKETWPGIDWQK
                     LSGYMIERVVSHGRRRAAEMREVSETLRSIGMEPTMAAAAAARQQWVADLGLKAKTED
                     RAVLVKAIREAMGK"
     gene            complement(16967..17557)
                     /locus_tag="VFB53_04615"
     CDS             complement(16967..17557)
                     /locus_tag="VFB53_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN86053.1"
                     /translation="MTRGVVDLEGLRSPQAEALRRMAPPAGLPFRVAKLGHVVLTVKD
                     MARAVAFYTGVLGFRVSDVYPDTMMKGGMVFLRCNGDHHCLALVGGAEEDASARELHH
                     LAFEVATLEELLRAREHLRKHKVKIDFDGRRRAGCQVAVEFRDPDNHFLELFWGLDQV
                     GSDQRVRPPEEWAEEHSLEDAIRHAPPGQDTTLHAK"
     gene            17604..19187
                     /locus_tag="VFB53_04620"
     CDS             17604..19187
                     /locus_tag="VFB53_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN86054.1"
                     /translation="MNVSDVMVRYLKAAGIGHVFGYPGDPNIEFMEAARRAGLEFVLG
                     RREGTAGLMAEAYGFLTGRPGVCMSTLGPGSSNLVNAVANAYLDRVPMIAISGQIERK
                     REQTFTHQVLNHNLIFTPVSKWATHVAPDTVGGVMRRALRTALAERPGPVHITTHADV
                     VGADAKDDALRLPPMAATAFGGDAGVAQRLRKAKQPVLLAGIAAQRANAHGAIARLAE
                     RAGIPVVVAPMAKGVLAEDHPYYAGTLDMACNQLMWDFLKGSDLLLAVGFDAVELIKP
                     WALSVPTVHVDALPNTDQIYSADTELVGDIAAIVEGIAAAWKGEPRSSAAAVKRHRDA
                     LRAAYYAGRVKGRLNPTDVIDAVREALPRQALATTDVGSHKLLVGQGWSAYEPRSVLM
                     TNGLSSMGYSLPAAIVAQLLNPRRPVVCFLGDGGLAMVQGELRLAAGLGLPLLVVVFC
                     DGSLNRIEIKQANRKYPSWGTLLEPTDVEMLARSMGCEGAAVDSARALERLLAAQRPQ
                     DRPLVVGARIDPAQYTQQF"
     gene            19204..20160
                     /locus_tag="VFB53_04625"
     CDS             19204..20160
                     /locus_tag="VFB53_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86055.1"
                     /translation="MKRIVVAVVAGLLAGAVQAQNWPTKPIRLLVPFAPGGSSSIVAR
                     SVAVEMEKGLGQPIVIDNRPGGGGNVAMQEAARAEPDGYTLIIGHIGTLAVNPFMFEK
                     LPFDTNADFAPVSLLAKVPSIFVVHADVPAKDLREFVALAKSQPGRLYYGSAGNGSAG
                     HLAMEYLKLVSGIDIVHVPYKGTGPNLIDLVAGRTQATAAGTPPLMPHVKAGKLRVIA
                     VGSQRLGSLPGVASVAEQGYPGFETTQWYGLNAPAKVPAAIIKRLADEAAKAARQPSV
                     AERFAVDDALAVGSTPQEYAAFIAAEQKRWGEVVRKANIKAN"
     gene            20160..21482
                     /locus_tag="VFB53_04630"
     CDS             20160..21482
                     /locus_tag="VFB53_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN86056.1"
                     /translation="MRVVERFAAWAARSRAAPLAPEVLHHAKRAVIDLHAAMLSGAVS
                     PPATLLEQALAEELDHGAARLALGRQATVRAAALINGAAAHTTEVDDIYRDGIYHPGA
                     PTIPAALALAQARGATGAQFLRAVVVGYEISTRIGAAMGRAHYRYWHNTGTIGCFGAC
                     AAAAELLQLDAARFAHALATVTTFSAGLQQSFRMDSMSKPLHAGRAAEAGITAALAAQ
                     EGITGSLDVIEGEAGYGRAMSDGPDWDRALATLGREFHVTRMTFKNHTCCGHTFAAID
                     GALALKAKMGLGADDIQRVRVGTYKAALDVAGYDEPRTAAEGRFSLKYVVATALTHGS
                     VRFAAFEPERLENPGTRELMRRVDVAVDPQLDAAFPAQRAARVAIEARDGRREEFYQP
                     TRIGDPDAPLSDSQLDAKYLELAAPVLGEAQAKALLGRLWRMEALESL"
BASE COUNT         3232 a         7479 c         7628 g         3218 t
ORIGIN      
        1 cggcgctcga gccgcaggtc ggcgaggggt tgcgcagcaa cgtgcgcgcc gtgttcgagg
       61 cgatcgccca ccgcaaccct tacccgcagg catggttcga tacgcaccgc tggaaccaca
      121 tggtgctcaa ggcgctgttc atcggcagcc cgctcgcgcc gatccagggg ctggacgcgc
      181 gcgccaacgc ggagctggcg cgcatcatgc gcgacttcgc gcacgagcgc tgggccgccg
      241 gccgcccggt gccgttcgag atctggcgct gcgtcgggcc gttcgccgaa gggccggcgc
      301 tggaggacct ggcgcgggtg ctgcgcaaag gcgaggcgat cgagaagcgc gcggcggcgc
      361 tcgccctggc ggcgagcccg gaccgcgagg cggcgcggct gctgcagacg gtgccggggc
      421 tcgccggcga catctcgctc aagcgcctga gctgggccac gctgcagtaa actcggcgca
      481 aggagacgcc cgccatgatg ctcatcgatc cgcatgcgca tatgatttcc cgcaccacgg
      541 acgactacga ggcaatggtc gcctccggcg tcgtcgcggt catcgagccg gcgttctgga
      601 tcggacagcc gcgcaccaac gtcggctcct acctggacta cctgtcctcc atcgtcggct
      661 tcgagcgctt ccgcgccagc cagttcggca tgcgccacta ctgcaccatc gggctgaatt
      721 ccaaggaggc caacaaccag gaactcgcag aaggcgtgat ggagctgatc ccgcgcttcg
      781 cgctgaagga gggcgtggtg gcgatcggcg agatcggcta cgacgagcag accccgctgg
      841 aggacaagta cttccgcctg cagctggagc tggcgaagaa gcttgacctg ccggtgatga
      901 tccacacgcc gcaccgcgac aagaagcgcg gcaccacgcg ctcgatggac gtgtgcgacg
      961 agcacggcct ggaccccgcc aaggtggtgg tggaccacaa caacgaggag accgtgcgcg
     1021 agaccctgga acgcggctac tgggcggcat tttccatcta ccccagcacc aagatgggca
     1081 atgcgcgcat ggtggagatc ctgcgccagt acggtgcgaa acaggtcatc gtcgactccg
     1141 cctgcgactg gggcatctcc gagccgctgg gcgtcgccaa gacggccaag cttgcactgc
     1201 agcaaggcat tcctgaaagc gatgtcaggt tggcctgcta cgccaatgcg ctgcgggcct
     1261 acggccagag cggccagatg aaggaagagg actggctcaa tccccaggcc atcgaccagc
     1321 gcgctgtctt tgaaggaaat tccatcctgc gcggcgggcg ggagccgcgc gtcgaggagc
     1381 gcaagggccg gcgcacgatg gacgacctga tcatcgagta gcgggatgcg aggccggtgc
     1441 ggcacaatgc cgcactgcgc aatgcgaggg aagcggccca cttccccgca gccgaaagga
     1501 tgtagaaaat tgtaaagccg ctacgtatac tgatttttcc cgcattgcct ggggaagagg
     1561 caattcaatc cactggaggt ggcaaatgaa tttcgcaaga accggtgcgg cgctgtccgc
     1621 cgccaccatg atcgcgtttt cggccggcgc gcaggcgcag gtcaagatca ggctggagcc
     1681 gctggtcacg ggaatcaata cgccgctcgc gatggtgcag ccggccggcg acgcccgcat
     1741 gttcatcatc gagcagaacg gccgcatcaa gatcctggaa ggcggcaagc tgcgcccgac
     1801 gccgtttctc gacatccgca gcaagattcc ggccttgcac cacgatttcg acgagcgcgg
     1861 gctcctcggc ctggcgttcc acccgaactt caagagcaac ggcaagttct acgtctccta
     1921 cagcgcgcac ctggactacg gcgcggacct cggccagatg ctgtggtact cgcacagcaa
     1981 cgtggtcgag gagtacacgg tgtcgaaggg cgacaagaac accgcggacc cgagctcggc
     2041 gcggcgcatc atgagcaccg cctggccgca gttcaaccac aacggccaat ggatcggctt
     2101 cggcccggac ggcaagctgt acgtctcgat gggcgacggc ggttacgcca acgactgggg
     2161 catcggccac aaccccgcca tcggcaacgg ccaggacatg tccacggtgc tgggcaagat
     2221 cctgcgcatc gacgtcgacg cgcgcaccgg cgacaaggcc tacggcatcc cgtcggacaa
     2281 cccgttcacc ggcgacaaga acgccaagcc cgagatctgg gcgctcggcc tgcgcaaccc
     2341 gtggcgctgc tcgttcaata cctccggcgg cgagctgttc tgcggcgacg tgcagcagaa
     2401 cagctatgaa gcgatcaaga tcgtcggcaa gggccagaac atgggctggc ggcgcgtgga
     2461 aggcatcatg cgctgcttcg actacctgaa gcccgacgat catccggcga actgcgacaa
     2521 ggccggcatc acgcccgcga tcatcgagta caacaactgc acggcgaagc cccagggctg
     2581 caaggggatc tcgatcaccg gcggctacgt gtaccagggc aaggaagcgg gctggaaggg
     2641 caagtacatc ttcggcgact ggagcaagtc cttcggcgag atggacggcc agatcttctt
     2701 cggcaccaag ggctccgacg gcaagtggag catggaggtc gccgaggtga ccaaccgcaa
     2761 gggcaagctg ccctacgtgc tggccttcgc gcaggaccgc aacggcgagg tgtatgcgct
     2821 gacctcgatc accaccggcc cgaacgggtc gctggacacg atctaccgca tcgtcccgtc
     2881 gaaataggca atcgcatgag cgccgccgcc gcgctccgcg cggcggcggt ttgcttttcg
     2941 ggttacattt cccgcatgag gatcgcagca tgaaagtgcg cacggcgtgc gccttgctcg
     3001 gcctgctcgc cgccgtggcg gcgctcgccc aggagcacaa ggccgtcaag gagccgctgc
     3061 ccgcgttcga caaggcctac ctgtcgaaca ggaagaacgt cgcggcaggc aaggacgtgt
     3121 gggacaacca gtgccggcac tgccacggcg cggcggccta tccgggcaag gcgcccaagc
     3181 tgaacccggg gcagctcgcg ccggacttca tctacgagcg ggtcacctac ggcttcggca
     3241 agatgcccgc ctggaaggac gtattctcgc tcgagcagcg caagagcgtc gtcgcctaca
     3301 tcaagagcag agatttctcg ccgtgacgcc ggctcaggcg gcgcgcttgc gcagcagcgt
     3361 ggtggccagg acggcgagca gcaccgcgaa tgcaatcatc atgcccatgc cctcgcgcgc
     3421 cgtttcctcc acccaccaca cgtccttcac ccagaaattg ggcatcgtcg gccagtagaa
     3481 caccgtgagg gccgcgccaa tcgccacgct cgcccacagc agccgctccc cggcgaggga
     3541 gcacacctgc gccggccgaa acgccgcggc gaaggcccag gcggcaggga caaggtagat
     3601 ggcgatccac accaaggcgt cggggtcgtt gtactggacg gcggcaaatg ccaccatcac
     3661 cacaccgggc agcacgagca ggtagcgcac aggggccatt ctaggacagg attcggcaga
     3721 tgacgacaga cgcccaaggg gcgcaggcgc gcactgacat ccactggcag cggttctcgg
     3781 tgccctacga gttcccggtc gtgttcaccg agggcgtgct cgacccggaa aacccggcgc
     3841 tgcgcgacgt gctgtgccgg ctcgagcccg gcaagcgcca tcgcgccgtg ttcttctacg
     3901 acgacggcct gaacgcgggc cgcgccccgc tcgccgaggc gatccagaac tatgcgcagc
     3961 gccatgcgga agcgatcgag ctcgcctgcg cgccgatctc gctgcccggc ggcgagaaga
     4021 tcaagtccga gctgcatttc gtcgagagca tccagcagac cctgttcgac ctgcacctcg
     4081 accgccattc ctacgtggtc gcgatcggcg gcggcgcggt gctcgacgcg gtcggcctgg
     4141 tggccgccac cacgcaccgc ggcgtgcgcc acgtgcgcat tcccaccacc gtactggcgc
     4201 agaacgactc cggcgtgggt gtgaagaacg gggtcaacct gcagggcgtg aagaacttcg
     4261 tcggcacctt tgcaccgccc ttcgcggtgc tgaacgacct cgcgttcgtc atgtcgctgc
     4321 ccgagcgcga caagatcgcc ggcatggcgg aggcggtgaa ggtggccctc atccgcgatg
     4381 ccgagttctt cggctggctc gagcgccaca tggacgactt ggcgaccttc gagcgccgcg
     4441 ccatggcgca catgatccgg cgctgcgccg agctgcacat gcgccagatc gggcagggcg
     4501 gcgatccctt cgagaccggc agcgcgcgcc ccctggacta cgggcactgg tcggcgcaca
     4561 agctggagag cctcacgcgg caccacgtgc gccacggcga ggcggtggcg atcggcatgg
     4621 cgctcgacgc gcgctactcg gtcctcgccg ggctgctggc cgcggggcag gaagagcgca
     4681 tctgcgcgct gctcgagtac ctcggcttca cgctgtggca cccggcgctc gccaaggccg
     4741 gcgccggcgg cgagtgggcg atcctggaag gcctgaagga cttccaggag cacctcggcg
     4801 gcgagctgac ggtgacgctg ctcgggggca tcggcaccgg cgtcgaagtg cacgagatcg
     4861 acgtcgcgcg catgcgcgag gcgatgcact ggctgaagga tcgcgccggc gcctgatgca
     4921 ccttgccgcg tctgccgcgg ggcgcgacct cgggcatctc acctactgca ccaacatcca
     4981 cgccggcgag gcgctgcagg aggtgcaggc gggcctcgcg cgctacctgc cggagatcaa
     5041 ggccggcgtg tcgccgggcc ggcccttcgg cgtcggcctg cgcctcggtc acgccgccgc
     5101 cgaaggcctg cgcgatcgcg ccgcgctcga ggcgctcaag cgctttctcg ccgaaggcgg
     5161 ctattacgtg ttcaccctca acgcctttcc gtacggcgcg tttcacgggc gcgcggtgaa
     5221 ggaggacgcc taccgccccg actggagcga gcccgaacgg cttgcctaca ccgatcacgc
     5281 cgccgacctg ctgagcgagc tgctgccggc gggcgccgaa ggcagcgtca gcacggtgcc
     5341 ctgtaccttc aagccctggg ccgcggggcg catggaggcg atcgtcgagc acctgatccg
     5401 ccacgtcgcg cacctggtgg ccttgcgcgg gcgcaccggg cagacgatcg ccctcgcgct
     5461 ggagcccgag ccgtactgct acctcgagac catcgaagag acggtggcgt tcttcgaggc
     5521 gcacctgttc catcgcgcgg gcgtcgcgcg gctggcggcg ctgaccggcc ttggtcccgg
     5581 cgcggccgag gcggccatgc gccggcacat cggcgtgtgc tacgacgtct gccatgccgc
     5641 ggtggagttc gaggacgcaa gggccagcgt ggcgcggctg cgcgccgccg gcatcgcgat
     5701 cggcaagctg cagctcagct cggcgctgcg cgtggccgcg ctcgacgcgc agagcgcgca
     5761 gcacctcgcc gcctttgccg agcccgtgta cctgcaccag gccgtggagc gctcgggcgg
     5821 ccggctgcgc cgctacctgg acctgcccga ggcgctgggc gccgccgccc ggggagcgga
     5881 gctgcgcgtg cacttccacg tgccggtgtt cctcgagcag atgcagaatt tcggcaccac
     5941 gcaggcgttc ctcgccgaga tcctggccct gcaccgcgcc gagccgatca gccggcacct
     6001 ggaggtcgag acctacacct gggacgtcct gcccgcgtcc taccgcggcg ccggcctggg
     6061 cccggcgatc gcgcgcgagc tgaactgggt caaggcgcag ctgctgcagt gaagctcggc
     6121 accgcgctgc gcctggggcg cgtctccaac ctgccgacgg tctggaccaa cacgctggcg
     6181 ggcgcggtgc tcgccggggc aggcgcctac ggcgcgcggt tcgcgctcat gctgctggcg
     6241 ttttcgctgt tctacaccgg cggcatgttc ctcaacgacg ccttcgacgc gcgcatcgac
     6301 gccgtgcagc ggcccgagcg tccgattccg tcgggcgaca tcggcgcgcg cgcggtgttc
     6361 gcctggggct acggcatgat ggcgctcggc acggtcatgc tggcgtggtt cggcgtggcg
     6421 agcgcgctcg ccgggctgac gctggcggtg gccattacct tctacgactg gcaccacaag
     6481 cagaacgtgt tcgggcctgt ggtgatgggt ttgtgccgcg tgctggtcta cgtcggcgcc
     6541 ggcctgtgca tcacgctcgc gttgccggac cggctgtggc tcggcgccgc cctgatatgg
     6601 tgctacctgg tcggcctgac ctacgtcgcc aagcaggaaa atctggaccg ggtggagaac
     6661 ctctggccgc tcctgttcct cgccgcgccg gtggccggcg gcgcctggct cgccgcggcg
     6721 cagccggcgg ccggcgtctt ctggctgctg tttaccggct ggatgctggt ggcgctgtgg
     6781 ttcctgcgcc ggcgcgcgaa aggggacgtg ccgcgcgccg tggtgagcct gatcgcgggc
     6841 atcgcgctgc tcgacgcgct gctgatcgcc gccgcgggcg cgccggcgct cgccgcgctg
     6901 gccctcgccg gattcgccgt cacctggttc ctgcagcgct acatcgccgg gacctgaacg
     6961 gcgggccatc cggggaaagc gcttgccagc gcccggcctt ttgtccatat taccgctgcc
     7021 gcggcgcgag gcgccgcgca tccaggagga gggaagatga ataccaagac gacgctgacg
     7081 ctggcgttca ccgccgcgtc gctcattgcc gccggcgcgc aggcgcagac ggtgaagatc
     7141 ggggtggtgc tgccctatac gggggtgggc gccgaattcg gccagcaggt ggaccgcggc
     7201 atccagatgt tcctcaagct caacccgacc gccttcggcg gccacaaggt cgagctggtg
     7261 aagcgcgatt cgaagaatcc gagcggggcg gaggccaaga ccgcggtgca ggaactgatc
     7321 gcgcaggaca aggtggacct gctcaccggc ttcatctact cgcccgacgc catcgcctcg
     7381 gcgccgctgg tgagcgcggc gaagaagccc atggtggtga tgaacgccgg caccgcctgg
     7441 atcaccaacc tttcgccgat gatctcgcgc gtgtccttct ccatgtggca ctcggggcac
     7501 gccatgggcg aggccgcggc gaagctgctc aaggcgaaga ccgcggtggt cggctacagc
     7561 gactttccgc cgggcaagga cagcctggac gccttcatgc gcggcttcga ggcgcacggc
     7621 ggcaaggtgg tcgacgccat cccgatgggc ggccccgggc aggtgcccga cttcacgccg
     7681 ttcttccagc gcgccaagga ccgcaagccc gacgtgttct ttgtcttcgt gccggcgggc
     7741 gaccatgcgg tgagcgtggt gaagacctac gccgcgctcg gcatgcgcca ggccggcatc
     7801 aagctgatcg gcccgggcga catcacgcag gacttcaagc tgcaggcgat gggcgacgac
     7861 gcggtggggc tgatcaccat gcaccactac aacgccgacc tcgacaacgc ggccaaccgg
     7921 cgcctggtgg cggcctggcg gcaggaatac ggcgccaacg cggtgcccga cttcatgggc
     7981 gtgcagggct acgacggcat ggcggcgatc gcgcacgcgg tgaccgcgct gaaaggcagc
     8041 atggaccccg acaaggtgca ggcggcgctc aagggctgga agtacgccga cagcccgcgc
     8101 gggccgatca gcatcgaccc ggcgacgcgc gacatcgtga tgaacgagta cctgagcgag
     8161 ctggtgaaga aggacgggcg cctgtaccag aagaacatcg gcgtgatcac cgccgtgaag
     8221 gacatgtgca aggaactcaa gcagggcaag tgcgcgaact gacgccgggc ccgtccttgt
     8281 agatggatct cgccagcatc ctgctcggcg ggctcgccgc cggcatggtg ctgttcgtgg
     8341 tgtcggtggg cctgtcggtc acgatgggcc tgatgggctt cgtcaacctg gcgcacggcg
     8401 gcttcgccat gctggggggc tacgcggtgg tgctggccat gcgccactgg gggctgccgt
     8461 tctcggcggc gctggctttc ggcttcgctg ccatcgccgc cctgtcggtg ctgctggagc
     8521 gcacgctgta cgcgcgcctg taccgcgcca gcgagctcga ccaggtgctg ttcaccatcg
     8581 gcctggtgtt cgtgatgatc gcctcggtca cgctcgccgt cggcccggag tcgcagcgcg
     8641 tcgagctgcc ggcctggctg gacggccaga ccgacctcgg cttcctgcgc taccgcagct
     8701 acagcctggc gctgatcgtc gccggcacgc tgctgatgct ggcgctgtgg ctgggcttcg
     8761 agcgcacgcg caccggcgcg aaaatccgcg ccgcggtgga caaccgccgc atggccgagt
     8821 cgctcggcgt ggacgtcggg cgccttttca ccctcacctt cgcgctcggc agcggcctgg
     8881 cggcggtggg cgggggtctc ggcgccgaga tcctcggcct cgacccgcag tatccgctgc
     8941 gctacctcgc ctacttcctc atcgtggtgg cggtgggagg cctggggcgc gtcaccggcg
     9001 tgttctgcgc cgcgctgctc atcggcgtgc tcgacttcac gctgaagatc tatttcccca
     9061 agggcggcac gatgttcatc tacatgctca ccctcggctt cctgctggcg cggccgcagg
     9121 gcctgttcgg caagcggggg acatgagctc ggccgcgatg catgagctgg cgatgcgcca
     9181 ccggctgcaa tggtgggagg cgctgccctg ggcggcgggc cttgcggcgt tcttcctgtt
     9241 tccgaactac ctcgccttcg gcacgcaggt gctggtgacg atcctcttcg cgctgtcgct
     9301 cgacctggtg ctgggctacg ccggcatcgt caccctgggc cacgccgcct tcttcggcgc
     9361 cggcgcctat gccgtgggca tgctcgccgc gcacgccggc tggaacgagc cgctcagcgg
     9421 cctcgtcgcc gcggcgctgg tggcgggcct gctcggcctg gtgagcggct ggatcctgct
     9481 gcgcacgcac ggcctgacgc tgctgatgct gaccttgtgc accatggcgc tgctggagca
     9541 ggcggcgaac atgttctccg gctggaccgg cggcttcgac ggcatgccgg gcctgtcgtt
     9601 cacgccgatc ctcggcctgt tcgagttcga cgtgctctat ttccgcacgc agtacctgta
     9661 cgcgctggcg gcgctgctgc tcggtttcgc cgtggtgcgg gtgctggtgt actcgccctt
     9721 cggccgcagc ctcaccggca tccgcgagaa cctgctgcgc atgcacgcgg tgggcgcgcc
     9781 ggtgcgcgcg cgccaggtgc tggtgtacgc catctcggcg gcgctcgccg gcgtcgccgg
     9841 cgcggtgttc gtgcaggcga ccggctacgt caacctcgcg gtgctggggc tggagcgcgc
     9901 cgcgggcgtg ctcatcatcc tgatcctcgg gggctacggg cggctgtacg gcgccttcgt
     9961 gggcgcgctg gtctacatgc tgctcgagca caccctgtcc aaggcctacc cgaccgcctg
    10021 gcaactgggg ctcggtctca cgctgatgct ggtggccctg ttcgcgcgca acggcatctt
    10081 cggcctgggc gacgccctgc gccgccgctg gagcgcgcgg tgagcgccgc gctgctggag
    10141 acgcgcgggc tgtgcaagag cttcggcgct ctggaagtgg cgcgccgcat cgacttccgg
    10201 ctgcaggcgg gggcgcgcca cgccctcatc ggcccgaacg gcgccggcaa gaccaccttc
    10261 gtcaacctgc tcaccggggt gctcgccccc tccgccgggc gcgtgctgct tgacggcgag
    10321 gacgtgacgc gcctcgcgca ggcgcggcgc gtgaagcgcg gcatcgcgcg cacattccag
    10381 atcaaccagc tgttccgcgg gctcacggtg ctggaaaacg tgtgcatcgc ggtcgccgag
    10441 cgcatgcgcg cgggcggcgg catgctgcgg cccgcgggcc agcgcgccga ggtggttgcg
    10501 gaagccgcgc gcctgatcgc gctgctgcag ctggatgccg acgcgggccg gcgcgtgggc
    10561 gagctgccct acggacgcca gcgcctggtg gagatcgcca tcgccctcgg gctgcagccc
    10621 cgcgtgctcc tgctcgacga gccggccgcc ggcatcccga gcgccgagag ccacatcctg
    10681 ctcgaggcga tcggcgcgct gcccgcggac atcgcggtgc tgatcatcga gcacgacatg
    10741 gacctggtgt tccgcttcgc cgagcgcatc acggtgatgg tgggcggggc gatcttcgcc
    10801 gaaggcaggc ccgccgagat cgccgcgaac cgcgaggtgc gcgccgtgta cctgggtggc
    10861 gcgctcgatg gctgaggcgc tgcgcctctc cggcgtgtcg gccggctacg gcgagacgca
    10921 cgtgctcgag ggcatcgagc tcgcgcttgc cgcgggcgag agcctggcgc tgatcggccg
    10981 caacggcgtg ggcaagtcga cgctgctcgc caccctgatg ggccacacca cgctgcacgc
    11041 cggcagcatc gagctcgccg gcgcgcgcat cgaagcggcg ccgccgcacc ggcgctcgct
    11101 cgccgggctc gcctgggtgc cgcaggagcg cgagatcttt ccttcgctca cggtgcgcga
    11161 gaacctcgag ctcggggcgc gccccgggca ctgggacatg gcgcgcgtgt gcgggctgtt
    11221 tcccaacctc gcggaccggc tggagcacgg cggcacgcag ctctccggcg gcgagcagca
    11281 gatgctctcc atcgcgcgcg cgctgctcac caacccgtcg gtgctgctga tggacgagcc
    11341 gaccgaggga ctcgccccgg tgatcgtgca ggcgctgggg caggtgctga cgacattgcg
    11401 cgaggcgggc ggcctcgcca ttgtgctggt ggagcagaac agccgcgtgg ccctggagtt
    11461 ctccgagcgc acggtggtga tggacaaggg gcgcatcgcc tacgacggcg ccgccgagcg
    11521 cctgcgccgc gacccggagt tcctggcgag cctcatcgcg gcgcagtgaa ctagaatccg
    11581 cggcatggcc ctgtccgcgt tcaatttcgc gcgctggatc gacgagcacg cgcacctgct
    11641 caggccgccg gtcggcaacc agcaggtgtt cgccgaagcg ggcgacctgg tggtgatggt
    11701 ggtgggcggg cccaacgcgc gcaccgacta ccacgacgat ccctacgagg agttcttcta
    11761 ccagctgcgc ggcaacatgg tcctcaaggt gatcgaggac ggccggccgc gcgacattcc
    11821 gatccgcgaa ggcgacatcc tgctcatccc ggggcacctg cggcactcgc cgcaacggcc
    11881 ggaagccggg tcggtgggac tggtcgtcga gaaggtgcgg ccgcgggacg tgaacgacgc
    11941 cttcgagtgg tactgcccgc agtgctgggc gcgcgtgcac cgcgtcgagg tgaacgtgca
    12001 ggacatcgtc aaggacctgc cgccgctgtt cgagggcttc taccggagcg cgcggcgctg
    12061 cccgcaatgc gggcacgtcc acccgggcaa gggatgaacc cgcgcgccat cgacatgcac
    12121 tcgcatttct tcccgcgcga gtgggaggac ctggggcggc gcttcggcac gcccgactgg
    12181 ccgtcgatga agcacttggg cgacggccgg gcgatggtga tgctgggcga gcgggagttc
    12241 cgcccggtga cctcggcctg ctgggacgcg ggccggcgcc tggaggagat ggaccgcgac
    12301 ggcgttgact tgcaggtgat gtgctcgacg ccggtgctgt tcgcctacgc gcgcccggcg
    12361 gcgcaggcgc tccactgcgc gcagctgttc aacgatgcgg gcctggcgct cgccgcgcgg
    12421 ggcaaggggc gcctcaagac cctgtgccag gtgccgctgc aggacaccga cgccgcgtgc
    12481 aaggagctgt cgcgggcaat gcgggccggc cacctcggcg tgcaaatcgg caaccacgtc
    12541 ggcaaccgcg acctcgacga cgagggcatc gtcgccttcc tgcagcattg cgcggccgaa
    12601 ggcgccgcgg tgctggtgca tccctgggac atgatgggcg gggcgcgcac gtcgcgctgg
    12661 atgatgggct ggacggtgtc gatgccggcg gagacgcagc tctcgatcgt gtcgctgatc
    12721 ctcggcgggg gcttcgaccg gctgccgaag gacctgcgcc tgtgtttcgc ccacggcggc
    12781 ggcagcttcg ccttcctgct cgggcggctg gaaaacgcct ggcacaaccg cgacgtggcg
    12841 cgcggcctgt cggagcatcc gccgagccat tacctcgacc ggttctccgt ggacagcgcg
    12901 gtgttcgacc cgcgggcgct gcgcctgctg atcgaggtga tgggcgagga ccgggtgatg
    12961 ctgggctccg actacccgtt cccgctgggc gagcagcgca tcggcaagct ggtccgcgac
    13021 atggaggacc tgggcgcggg cgcgcgccgg aagctcctgg cgggaaacgc gaagcgcttc
    13081 ctcggcctct aggcgccctt cagcgccgcc agcgcgcgga tgatctcctc ggtctccggg
    13141 tagcggccgt cgcggtgctt ggagaacacc agcttgccgt cgcaggcgac gtcgaacacg
    13201 ccgcccttgc ccttggcgag ctcggcttcg atccccaacg cctgcttgat ctgggcagcc
    13261 agaccggccg cccggggcag gtagtttcag ggaacgcagt aggtgatgac gacctgcatc
    13321 gcgccgtcat tctatactcg cgctgccgtg aagatcaccg tcctcgacga ctacttcgac
    13381 accgtgcgca tgctcgagtg cttcgcgaag ctcgcgggcc acgaggtgac ggtgtggaac
    13441 gaccacctgc aggaaaccgg cggcctggcg gcgcgcctcg cggacaccga cgcgctggtg
    13501 ctgatccgcg agcgcacgca gatccgcgcg ccgctcctcg agcgcctgcc gcggctgcgc
    13561 ctcatcagcc agaggagcgt gtatccgcac atcgacatcg acgcctgcac gcgcctgggc
    13621 gtggtggtgt cctccagcca gcacgcgggc acgccttcct atgccgccgc cgagctcacc
    13681 tggggcctcg tcctcgcggc cgagcgatcc atcccgcagc agatggcctc gctcagggcg
    13741 gggcgctggc agatgggcgt gggcaacagc ctgcgcggca ggacgctcgg catccatggc
    13801 tacggccgca tcggcagcgc ggtggcgggc tacggccggg ccttcggcat gaaggtcatg
    13861 gtgtggtcgc gcccggattc gctggcgaag gcgcgggccg acggctacgc gacggcgtcg
    13921 agcaaggaag agttcttcgc gaactgcgac gtgatcacgc tgcacctgcg tctgatcgag
    13981 gccacgcgcg gcatcgtcac cgccgccgac ctcgcgcgca tgaaaccgga ctcgctcctc
    14041 gtcaacacca gccgggccgg gctcatcgcc cccggggcgc tcgttgcggc gctgaaagcg
    14101 ggccgcccgg gccgcgccgc ggtggacgtg ttcgagaccg agccgctgcg cgacacggcc
    14161 gacccgctgc tcgccatgga caatgtcatc gccacgccgc acatcggcta cgtgacgcgc
    14221 gaggagtacg agctgcagtt caccgacatc ttcgaccaga tcctcgccta cgcggccggc
    14281 aggccgatga acgtcgtcaa ccccgcggcg ctcgccgccc gaagcgcggg tacacgcgcc
    14341 tgacgttgcc cgaatagatt ttttcccggt cggcggcgct caagttcccg accgcgtcga
    14401 tgtagcgccg cgtgtcgtcg tagtagtgcc cggtctccgg gtcaacgccg cgcaccgcgc
    14461 ccacgatctc ggaggcgaag aggatgttct ccgccggaat caccttcagc agcagctcga
    14521 tgcccggcag gtggtacacg caggtgtcga agaagacgtt gccgagcagc agatccttga
    14581 gcccaggctt gcccatgtcc tgcgcgaggc cgcggtagcg cccccagtgg tagggcaccg
    14641 cgccgccgcc gtgcggaatg acgaatttca gcgtcgggaa gtccttgaac aggtccgact
    14701 gcaggaactg catgaacgcc gtggtgtcgg cgttgaggta atgcgcgccc gtgaagtgga
    14761 aggccgggtt gcacgaggag ctgacgtgca ccatcgcggg cacctcgagc tccacgagct
    14821 tgtcgtagag cgggtaccag aaccggtcgc cgagcggtgg cgacttccac cagccgccgg
    14881 aagggtcggg gttgaggttg cagccgatga agccgagctc cttcacgcag cgctcgagct
    14941 cggggatgca gttcgccgga gggacgcccg gggactgggg aagctggcac accggggcga
    15001 acctggacgg atacaggctg cacacgcgat ggatcaggtc gttgcactgc cgcgaccagt
    15061 cctcgctcat gcgcgcgtcg ccgacgtgat gctccatgga gcccgcgcgc ggcgagaaga
    15121 aggtgaggtc ggtgccgcgc tcgcgctgga tgcgcagctg gttcttctcc aggctgtcgc
    15181 ggatctcgtc gtcggaaata cgcaaggggc ccgggtcccg cccggcggcc tggagcttcc
    15241 tccactccca cagggaggcg ggcgcggtgg tgtagtggcc gtggcagtcg atgatcatgc
    15301 gaagagttct tccaccgcgt agcgccgcgg gatgagctgt tggcggtgca cgtagtcgat
    15361 cgcgacttcg aggttgcggc ggttggcttg cagcccgatc ggattcagct ccgggttgcc
    15421 cgcggcggcg aagctctctt cgagccgtcg caggatcccg tccgcatccg cccgcgacac
    15481 ggagtccttc accgccacca tgtggttgat ctggatcgcg ccggtgcgcg cgcgccaggc
    15541 gcgcgcggcg ccctcggcgt ccgggatcaa cggcacgatg cgcgggtccg tggccggcgc
    15601 gtcgccactg acggcggcgt cgacctcgcc cgcgagcagc atctcctcca gcttgcggcc
    15661 ggcgggcgcg cgttcgacgt tggccgggtc gcggaattcc gcgacatgcg cctcctcgaa
    15721 ggtcacccag gtgatccgcc ggtggtccac cgcgtagtcc tcctcgagga tgccgcggat
    15781 ccacatgccg gtggtcacgg agtacgaacg tacgccgatg cgcttgccct cgagactgcg
    15841 gggcgcgacc gggcgcgtgc ggtcgcagac caggcgcgga tgctgcatgc gcgcgagcac
    15901 cacggcgggg agcagccggt agggcttgcc cgccgccttc gccatgagaa aggtggtgat
    15961 ggcgagctcg gcgaaatcga actcgaggtt gcgcaccacg cgcttgaagc ccgcgcttgg
    16021 cgggcgcact tcctcgaacg cgagcggcgc ggtgcgcttg aactgggccg tgcaggggta
    16081 gtcgccgagc agcgccttca tttgcccatc gcctcgcgga tcgccttcac cagcacggcg
    16141 cggtcctcgg tcttcgcctt caggcccagg tccgcgaccc actgctgccg cgccgccgcg
    16201 gccgccgcca tcgtcggctc catgccgatg ctgcgcagcg tctcggagac ctcgcgcatt
    16261 tcggcggcgc ggcgcctgcc gtggctcacc acgcgctcga tcatgtagcc ggagagcttc
    16321 tgccagtcga tgccgggcca ggtctccttg agcgaggcga gcacgcggtc ctcgaccccg
    16381 tattcggcgg cggcggtcat cgactcgacg agcagcgcct ccagcccctt caccacgatc
    16441 gagcggaaca tcttgatcgc cgaggcctgg ccgacctcgg ccgagacgaa ctccatctgc
    16501 atgccgagcg cggccgcacg cggcgcgagc gccgcggcgc cggggccgcc gatgaggcag
    16561 ggcaccttgt gcaggtaggg cgccgccggc gccatcaccg cgacgtcgac gtaggccgcg
    16621 ccggtgggcg cgaccagcgc cgcggtttcg atcttgcgcc tgggcgagac cgagttcatg
    16681 tcgaggaaca gctggtcctt gcgcaggccc ggcaggcagg accttgccgc ctcgaggctg
    16741 gaggcagcgg tcaccgtgag catcaccagg tccgactcgc gcaccgcgtc ggcgtggtcg
    16801 cgcgccaggg ccacgcccag cggcttcgcc gccgcctcga gcggcgcgcc cgccggctgc
    16861 ccgaacagga tgtcgtaggc gcggatcgcg ggcttcgaac tgccggccag accgcggctg
    16921 atcgtctggc ccgcttcgcc gaagccgatg aaggcgatgc gctcgctcat ttcgcgtgca
    16981 gcgtggtgtc ctgcccgggc ggcgcgtggc ggatggcgtc ttccagcgaa tgttcctccg
    17041 cccactcctc gggcgggcgc acgcgctggt cgctgccgac ctggtcgagg ccccagaaga
    17101 gctcgaggaa gtggttgtcg gggtcgcgga actcgaccgc cacctggcag ccggcgcggc
    17161 gccggccgtc gaagtcgatc ttcaccttgt gcttgcgcag gtgctcgcgc gcgcgcagca
    17221 gctcctccag cgtcgccact tcgaaggcca ggtggtgcag ctcgcgggcc gaggcgtcct
    17281 cttccgcgcc gccgacgagc gcgaggcagt gatggtcgcc attgcagcgc aggaacacca
    17341 tgccgccctt catcatggtg tccgggtaca cgtccgacac gcgaaagccg agcacgccgg
    17401 tgtagaaggc caccgcgcgc gccatgtcct tcaccgtgag caccacgtgg cccagtttcg
    17461 cgacgcgaaa cggcaggccg gcgggcggcg ccatgcgccg cagggcttcg gcctgcgggc
    17521 ttctcaggcc ttcgagatcg acgacgcctc gcgtcatgaa cttcgcctcc tcaaagcgcg
    17581 gcagtttggc acaatccggg gcgatgaatg tctccgacgt gatggtgcgg tacctgaagg
    17641 cggccggcat cggccacgtc ttcggctacc cgggcgaccc gaacatcgag ttcatggaag
    17701 ccgcacggcg cgccggcctc gaattcgtgc tcgggcggcg cgaaggcacc gcgggcctga
    17761 tggccgaagc ctacggcttc ctcaccgggc ggccgggcgt ttgcatgtcc acgctcgggc
    17821 cggggtcctc gaacctggtg aacgcggtgg ccaacgccta cctcgatcgc gtgccgatga
    17881 tcgcgatctc cgggcagatc gagcgcaagc gcgagcagac cttcacgcac caggtgctga
    17941 accacaacct gatttttacc ccggtgagca agtgggccac ccacgtggcg cccgacacgg
    18001 tgggcggcgt aatgcgccgg gcgctgcgca ccgcgctggc cgagcgcccg ggcccggtgc
    18061 acatcaccac gcacgccgac gtggtcggcg ccgacgcgaa ggacgatgcg ctgcgcctgc
    18121 cgccgatggc cgccacggcg ttcggcggcg acgccggcgt cgcgcagcgc ctgcgcaagg
    18181 cgaagcagcc cgtgctcctc gccggcatcg ccgcgcagcg cgccaacgcg catggggcga
    18241 ttgcgcggct tgcggagcgc gccggcatcc cggtggtggt ggcgccgatg gcgaagggcg
    18301 tgctcgccga ggaccatccg tactacgccg ggacgctgga catggcgtgc aaccagctca
    18361 tgtgggattt cctcaagggc agcgacctcc tgctcgccgt gggtttcgac gcggtcgagc
    18421 tgatcaagcc ctgggcgctc tcggtgccca ccgtgcacgt cgatgcgctg ccgaacaccg
    18481 accagatcta cagcgcggat acggaactcg tcggcgacat cgccgcgatc gtcgagggca
    18541 tcgcggcggc gtggaagggc gaaccgcgct ccagcgcggc ggcggtgaag cgccatcgcg
    18601 acgccttgcg cgccgcgtac tacgcggggc gcgtgaaggg caggctgaac cccaccgacg
    18661 tcatcgacgc ggtgcgcgag gcgctgccgc gccaggcgct cgccaccacc gacgtcggct
    18721 cgcacaagct gctggtcggc cagggctgga gcgcctacga gccgcgctcg gtgctgatga
    18781 ccaacgggct gtcctccatg ggctactcgc tcccggcggc gatcgtggcg cagctgctca
    18841 accccaggcg cccggtggtg tgcttcctcg gcgatggggg cctcgccatg gtgcagggcg
    18901 agctgcggct cgccgccggc ctcggcctgc cgctgctggt ggtggtgttt tgcgacggga
    18961 gcctcaaccg catcgagatc aagcaggcga accgcaagta cccgagctgg ggtacgctgc
    19021 tcgagcccac cgacgtcgag atgctggcgc ggtcgatggg ctgcgaaggc gccgcggtgg
    19081 acagcgcccg ggcgctcgag cggctgctcg cggcgcagcg cccgcaggac cggccgctgg
    19141 tggtgggcgc gcgcatcgac ccggcgcagt acacgcaaca gttctgacca gggaggagag
    19201 cagatgaagc gaatcgtcgt ggcagtggtg gccggcctgc tggcgggtgc cgtccaagcg
    19261 cagaactggc ccaccaagcc gatccggctg ctggtgccct tcgcccccgg cggctcgagc
    19321 tcgatcgtcg cgcggtcggt ggcggtggag atggaaaagg gactcggcca gccgatcgtc
    19381 atcgacaacc ggccgggcgg cggcggcaac gtcgcgatgc aggaggcggc gcgcgccgag
    19441 cccgacggct acacgctgat catcggccac atcggcacgc tggcggtcaa tccgttcatg
    19501 ttcgagaagc tccccttcga caccaacgcc gacttcgcgc cggtgtcgct gctcgccaag
    19561 gtgccgagca tcttcgtggt gcacgccgac gtgccggcaa aggacctgcg cgagttcgtc
    19621 gccctggcga agagccagcc cggcaggctc tactacggct cggcgggcaa cggcagcgcg
    19681 gggcacctcg cgatggagta cctgaagctc gtctccggga tcgacatcgt gcacgtgccc
    19741 tacaagggca ccgggcccaa cctgatcgac ctggtggccg ggcgcacgca ggcgaccgcc
    19801 gccggcacgc cgccgctgat gccgcacgtg aaggcgggca agctgcgcgt catcgccgtg
    19861 ggctcgcagc gcctgggatc gctgcccggc gtggcctcgg tggccgagca gggctaccct
    19921 gggttcgaga ccacgcagtg gtacggcctc aatgcgccgg ccaaggtgcc cgccgcgatc
    19981 atcaagcgcc tggccgacga agcggcaaag gccgcccggc agccttcggt ggccgagcgc
    20041 ttcgcggtgg acgacgcgct ggccgtgggc tccacgccgc aggagtacgc cgccttcatc
    20101 gccgccgagc agaagcgctg gggcgaggta gtgcgcaagg cgaacatcaa ggcgaactga
    20161 tgcgcgtcgt cgagcgcttc gcggcctggg cggcccggtc gcgcgccgcg ccgctcgccc
    20221 ccgaggtcct gcaccacgcc aagcgcgcgg tcatcgacct gcacgcggcg atgctctcgg
    20281 gcgccgtgtc cccgcccgcc acgctgctgg aacaggcgct tgccgaggag ctggatcacg
    20341 gcgcagcgcg cctcgccctg gggcgccagg cgaccgtgcg cgccgcggcg ctgatcaacg
    20401 gcgccgccgc gcacaccacc gaggtggacg acatctaccg cgacggcatc taccacccgg
    20461 gcgcgccgac catccccgcg gcgctcgcgc tggcgcaggc gcgcggcgcc acgggcgcgc
    20521 agttcctgcg cgcggtggtg gtgggctacg agatctccac gcgcatcggc gccgccatgg
    20581 gccgcgcgca ctacaggtac tggcacaaca ccggcaccat cggctgcttc ggcgcctgcg
    20641 ccgcggccgc cgagttgctg cagctggatg cggcgcgctt cgcgcacgcg ctggccaccg
    20701 tcaccacctt ctcggccggc ctgcagcagt cgttccgcat ggattcgatg tccaagccgc
    20761 tgcacgccgg ccgcgccgcg gaggcgggca tcaccgcggc gctcgcggcg caagagggca
    20821 tcaccggctc gctcgacgtg atcgaaggcg aggcgggcta cggcagggcg atgagcgacg
    20881 ggcccgactg ggaccgcgcg ctcgccacgc tcgggcgcga gttccacgtc acgcgcatga
    20941 ccttcaagaa ccacacctgc tgcggtcaca ccttcgccgc catcgacggc gcgctggcgc
    21001 tgaaggcgaa gatgggcctc ggcgcggacg acatccagcg cgtcagggtc ggcacctaca
    21061 aggcggcgct cgacgtggcg gggtacgacg agccgcgcac cgcggccgag gggcgcttct
    21121 cgctcaagta cgtggtcgcg accgcgctca cccacggcag cgtgcgcttc gcggccttcg
    21181 agcccgagcg cctggaaaac cccgggacgc gcgagctgat gcgccgcgtg gacgtcgccg
    21241 tggacccgca gctcgacgcg gcctttcccg cgcagcgcgc ggcgcgcgtg gcgatcgaag
    21301 cgcgcgacgg ccggcgcgag gagttttacc agccgacgcg aatcggcgat cccgatgcgc
    21361 ccctgtcgga ttcccagctc gatgccaagt acctcgagct cgccgcgccg gtgctcggcg
    21421 aggcgcaggc caaggcgctg ctcggccgcc tgtggcgcat ggaagcgctc gagtcactgt
    21481 gatcgtcgtc cactgagatg gcgtttctcg cttcccgcgt cgcccgcgcg cgcatctcgg
    21541 cctcggccgc cagcgcg
//
LOCUS       DASRIE010000103         6433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2445913, whole genome shotgun sequence.
ACCESSION   DASRIE010000103 DASRIE010000000
VERSION     DASRIE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445913"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFB53_04635"
     CDS             complement(<1..373)
                     /locus_tag="VFB53_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZN86057.1"
                     /translation="MHAFNYHRPTNVNDAAAQAAKSADAKVLAGGQSLVQAMKLRLAA
                     PTDIVDIGMIKDLAGIKVDAKSVTIGAMTRHAEVAASAEVKKAIPALAALAGMIGDRQ
                     VRNMGTLGGSLANSDPAADYPA"
     gene            complement(384..2738)
                     /locus_tag="VFB53_04640"
     CDS             complement(384..2738)
                     /locus_tag="VFB53_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN86058.1"
                     /translation="MGAPLIGARIQRKEDYRFLTGAGQYTDDVTLPRQSFAAFVRSPH
                     AHAKIRSVNAAKAKGAPGVLAVYTGEDIAAAKLGGLPCGWLITDVHGQPMKEPPYPVL
                     AQGKVRHVGERVAVVIAETQDQARDAAELVQVDYEPLPAVADGRLARAKGAPQLHEIA
                     PDNTCYVWALGDKAAVDAAFAKAAHVTKLDFVNNRLIPNAIEPRAANGSYSRADDSYT
                     LYVANQNPHVERLLMTAFVMGLPEHKVRVVAGDVGGGFGSKIYLYAEDVVVTWASRQL
                     ARPVKWTAERSESYTSDAHGRDHHTVAELALDKDGKFLAMRVKTTANVGAYLSTFASC
                     VPTILYATLLAGQYTTPLIHCEVTAVFTNTAPVDAYRGAGRPEATYVVERLVETAARE
                     MKIDPAEIRRRNFIRSFPYQTPVALLYDTGNYDATMDAAQKLADVAGYPARKKASEAR
                     GRLRGMGYAAYIEACGIAPSAVAGSLGARAGLFEAGEIRVHPTGSVTVFTGSHSHGQG
                     HETTFAQVVADRLGMAIENVDIVHGDTSKILFGMGTYGSRSIAVGGTAIVKAMDKIIA
                     KGKKIAAHLLEAAEADIEYAGGVFKVAGTDKQKTFGEVAFAAYVPHNYPHDKLEPGLN
                     ENAFYDPSNFTFPAGTYVCEVEVDPETGKVQVDRWTAVDDFGRIINPMIVEGQVHGGL
                     AQGIGQALTEGAKYDSHGQLVTGSFMDYAIPRADDVPFFQVDTRETPCTHNPLGVKGC
                     GEAGAIGAPAALINAITDALGVKDIAMPATPQTVWRTLNQKKAS"
     gene            complement(2749..3228)
                     /locus_tag="VFB53_04645"
     CDS             complement(2749..3228)
                     /locus_tag="VFB53_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN86059.1"
                     /translation="MPVKVSMKVNGKAVSGEVEARTLLVTYLREHLRLTGTHVGCDTA
                     QCGACTVHMNGKAVKSCSMLAMQAEGADVLTIEGLAKADGTLHPMQAAFKEHHGLQCG
                     FCTPGMVMNAVDFAARHPGASEQDVREALEGNLCRCTGYHNIVKSIVAGSKAMAAGK"
     gene            3443..4270
                     /locus_tag="VFB53_04650"
     CDS             3443..4270
                     /locus_tag="VFB53_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86060.1"
                     /translation="MPRILVVEDDALLADGLARGLAQAGFAVTLCARGELALAALRRG
                     AMMDPYMRILIVEDDATLGSGLGRILQSEGYAVDLMARGEQAVQAVQRERFDLVILDV
                     GLPDIDGFEVLRRLRGGAQRMPVLVLTARDTIDDRVHGLDLGADDYLAKPFAMPELAA
                     RVRALIRRSQAQSGPRIVHGPLALDTVARRAFLHEAPLDLAAREWAVLEVLLARVERV
                     VSKESIIQAVASWGDELSANAIEVYVSRLRAKLEPAGIRIRTVRGFGYMLEEFRETH"
     gene            4267..5637
                     /locus_tag="VFB53_04655"
     CDS             4267..5637
                     /locus_tag="VFB53_04655"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZN86061.1"
                     /translation="MSARSLRTHLLRLLLPPVALTLAVGTVAAYYVSLEPGAEAYDQS
                     LINTAYAVGERIRVQERGYSLDLPSVAERLARSDRFDEVYYVVRGPDRERIAGDAGLP
                     LPPAGAEPADEVLAYDAAREGKRLRLVEVRVPCGGRSCTVTMAETTVKRQRLARDILA
                     SSALPLALLAVLTLALVWFGVARGLRPLQGLSDEIRARSPHDLRPLDATQAPDEARPL
                     VEGINRLLGEVAEANRNQQRFLANAAHQLRTPLAGLQAHTELALAQPVPQSCRAELEQ
                     VHGATVRTARLANQLLALARAEALARAPDGRPGANLRAVVEESADEWVRRALQRDIDL
                     GFELEDLTVAGDAFLLREALANLVHNALEYTPAGGRVTVRAARRGAQGAVLEVEDDGP
                     GIAPAERGRVLERFYRAPGTGGTGSGLGLSIVREIAAAHGALVAIGEGEGGRGCRVTL
                     QFAGVN"
     gene            5643..>6433
                     /locus_tag="VFB53_04660"
     CDS             5643..>6433
                     /locus_tag="VFB53_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN86062.1"
                     /translation="MDSVDIEVLRSAEAWRAAGQRVTLGTIVKTWGSAPRPVGALVVI
                     RGDGQVVGSVSGGCVEDDLIDKVRSQAVAKDRPQLLTYGVTNEEATRWGLPCGGTLQL
                     VLEPVGEASALGELLRTVSRQQLVKRRLDMQSGRVSLEPGQWQDALEFDGATLVTVHG
                     PRWRLVLIGAGQLTQYLAEMARMLDYHVSVIEPREEYASGWNVAGVELERRMPDDVIA
                     AMNLDGHSAVVALTHDPKIDDLALMEALKSAAFYVGAIGSKKNNDA"
BASE COUNT          840 a         2273 c         2333 g          987 t
ORIGIN      
        1 ccgccgggta gtcggccgcc gggtcgctgt tggcgagcga cccgccgagc gtgcccatgt
       61 tgcgcacctg ccggtcgccg atcatgccgg cgagggcggc gagcgccggg atcgccttct
      121 tcacttcggc cgaggcggcg acttcggcgt ggcgcgtcat ggcgccgatg gtgacgctct
      181 tggcatccac cttgatgccg gcgaggtcct tgatcatgcc gatgtcgacg atatccgtcg
      241 gcgcggcgag gcgcagcttc atcgcctgca ccagcgactg gccgccggcg aggaccttgg
      301 cgtccgcgct cttcgccgcc tgcgcggcgg cgtcgttcac gttggtggga cggtggtagt
      361 tgaatgcgtg catggtggtg tcctcaggac gcttttttct ggttgagagt gcgccatacg
      421 gtctgcggcg tggccggcat ggcgatgtcc ttcacgccca gggcgtcggt gatggcgttg
      481 atcagcgccg ccggcgcgcc gatggcgccc gcctcgccgc agcccttcac gccgagcggg
      541 ttgtgcgtgc agggcgtctc gcgcgtgtcc acctggaaga acggcacgtc gtcggcccgc
      601 ggaatggcgt agtccatgaa cgagccggtg accagctggc cgtgcgagtc gtacttcgcg
      661 ccctcggtga gcgcctggcc gatgccctgc gccaggccgc catgcacctg accctcgacg
      721 atcattgggt tgatgatcct gccgaagtcg tccaccgcgg tccagcggtc cacctgcacc
      781 ttgccggtct ccgggtccac ctccacctcg cagacgtagg tgccggccgg gaaggtgaag
      841 ttcgacgggt cgtagaacgc gttctcgttc aggccgggct ccagcttgtc gtgcgggtag
      901 ttgtgcggca cgtaggccgc gaacgccacc tcgccgaagg tcttctgctt gtcggtgccg
      961 gcgaccttga acacgccgcc cgcgtactcg atgtcggcct cggcggcctc cagcaggtgc
     1021 gccgcgatct tcttgccctt ggcgatgatc ttgtccatcg ccttgacgat ggcggtgccg
     1081 cccacggcga tggagcgcga gccgtaggtg cccatgccga acaggatctt cgaggtgtcg
     1141 ccgtgcacga tgtccacgtt ctcgatcgcc atgcccagcc ggtcggccac cacctgcgcg
     1201 aaggtcgtct cgtggccctg gccgtgcgaa tgcgagccgg tgaacaccgt caccgagccc
     1261 gtcgggtgca cgcggatctc gcccgcctcg aacaggccgg cgcgcgcgcc gagcgatccc
     1321 gcgaccgccg agggcgcgat gccgcaggcc tcgatgtagg ccgcgtagcc catgccgcgc
     1381 agcctgcccc tggcttccga ggccttcttg cgcgccggat agccggccac gtcggccagc
     1441 ttctgcgccg cgtccatggt ggcgtcgtaa ttgccggtgt cgtagagcag ggccaccggc
     1501 gtctggtagg ggaagctgcg gatgaagttc ctgcggcgga tttccgccgg gtcgatcttc
     1561 atctcgcgcg ccgccgtctc caccaggcgc tcgacgacgt aggtcgcttc cgggcgcccc
     1621 gcgccgcggt aggcgtccac cggcgcggtg ttggtgaaca ccgcggtcac ctcgcagtgg
     1681 atcagcggcg tggtgtactg gccggcgagc agcgtggcgt acaggatcgt cggcacgcag
     1741 gaggcgaagg tcgacaggta ggcgccgacg ttggccgtgg tcttgacgcg catggcgagg
     1801 aacttgccgt ccttgtccag cgccagttcg gcgacggtgt ggtggtcgcg cccgtgcgcg
     1861 tccgaggtat aggactccga gcgctccgcg gtccatttca ccgggcgtgc gagctgcctg
     1921 gaggcccagg tcaccaccac gtcctccgcg tacaggtaga tcttggagcc aaagccgccg
     1981 ccgacgtcgc ccgccaccac gcgcaccttg tgctcgggca ggcccatcac gaaggcggtc
     2041 atcagcagcc gctcgacgtg cgggttctgg ttggcgacgt acagggtgta gctgtcgtcg
     2101 gcgcggctgt aggaaccgtt ggccgcgcgc ggctcgatgg cgttggggat caggcggttg
     2161 ttgacgaagt cgagcttggt gacgtgcgcc gccttggcga aggccgcgtc gaccgccgcc
     2221 ttgtcgccca gcgcccagac gtagcaggta ttgtccggcg cgatctcgtg cagctgcggc
     2281 gcgcccttgg cgcgcgccag gcggccgtcg gccaccgcgg gcagcggctc gtagtccacc
     2341 tgcaccaatt cggcggcgtc gcgcgcctgg tcctgcgtct cggcgatcac caccgcgacg
     2401 cgctcgccga cgtggcgcac cttgccctgc gccagcaccg ggtagggcgg ctccttcatc
     2461 ggctggccat ggacatcggt gatcagccag ccgcagggca ggccgccgag cttggcggcg
     2521 gcgatgtcct cgccggtata gacggcgagc acgcccggcg cgcccttcgc cttggcggcg
     2581 ttcaccgacc tgatcttcgc gtgcgcgtgc ggcgagcgca cgaacgcggc gaagctctgg
     2641 cgcggcagcg tcacatcgtc cgtgtactgg cccgcgccgg tgagaaagcg gtagtcctct
     2701 ttgcgctgta tgcgagcgcc gatcagcgga gcacccatgg gcgcctccct acttgcctgc
     2761 ggccatggcc ttggatccgg ccacgatgga cttgacgatg ttgtggtagc ccgtgcagcg
     2821 gcacaggttc ccttcgagcg cctcgcgcac gtcctgctcg ctcgcgcccg ggtggcgcgc
     2881 ggcgaaatcc accgcgttca tcaccatgcc cggcgtgcag aatccgcact gcaggccgtg
     2941 gtgttccttg aacgcggcct gcatcgggtg cagcgtgccg tcggccttgg ccaggccctc
     3001 gatggtcagc acgtcggcgc cttcggcctg catggcgagc atcgagcagg acttcaccgc
     3061 cttgccgttc atgtgcacgg tgcacgcgcc gcactgcgcg gtgtcgcagc cgacgtgcgt
     3121 gccggtgagg cgcaggtgct cgcgcagata ggtgaccagc agcgtgcgcg cctcgacctc
     3181 cccggagacg gccttgccgt tgaccttcat ggataccttg actggcatgc tgcttcctcc
     3241 tcggaccgat cgattgtttg ctgctggggg aaaggtcatc ctagccaagc cgattcgccc
     3301 cggcaatgtt gcgccgcaat aaccggacct ccctgcgcgt aagcaccccg caagcttgca
     3361 gcaaggcccc ggcaaactgg cgcccctacc ttggggcgcg atgatcgccc ggcacgccgc
     3421 ccccgcccct gccaccgcgc gcatgccgcg catcctggtc gtcgaggacg atgcgctgct
     3481 cgccgacggc ctggcgcgcg ggctggcgca ggcgggattc gccgtcaccc tgtgcgcgcg
     3541 cggcgaactg gcgctcgcgg cgctgcgacg cggcgctatg atggaccctt acatgcgcat
     3601 actgatcgtc gaggacgacg ccacgctcgg cagcggcctg ggccgcatcc tgcaatccga
     3661 gggctacgcg gtggacctga tggcgcgcgg cgagcaggcg gtgcaggcgg tgcagcgcga
     3721 gcgcttcgac ctggtgatcc tcgacgtcgg cctgcccgat atcgacggct tcgaagtgct
     3781 gcggcgcctg cgcggcggcg cgcagcgcat gccggtgctg gtgctgaccg cgcgcgacac
     3841 gatcgacgac cgcgtgcacg gcctcgacct gggcgccgac gactacctgg ccaagccttt
     3901 cgccatgccc gaactcgccg cgcgcgtgcg cgcgctgatc cgccgctcgc aggcgcagag
     3961 cggcccgcgc atcgtgcacg ggcccctggc gctcgacacg gtggcgcggc gcgccttcct
     4021 ccacgaggcg cccctcgacc tggcggcgcg cgagtgggcg gtgctggaag tgctgctggc
     4081 gcgcgtcgag cgcgtggtgt ccaaggaatc catcatccag gcggtcgcca gctggggcga
     4141 cgagctgtcg gcgaacgcga tcgaagtgta cgtgtcgcgc ctgcgcgcga agctcgagcc
     4201 cgccggcatc cgcatccgca cggtgcgcgg cttcggctac atgctggagg agttcaggga
     4261 gacgcattga gcgcgcgcag cctgcgcacg cacctgctgc gtctgctgct gccgccggtg
     4321 gcgctgactt tggccgtcgg cacggtcgcg gcctactacg tctccctcga gccgggcgcc
     4381 gaggcctacg accagtcgct gatcaacacg gcctacgcgg tcggcgagcg catccgcgtg
     4441 caggagcgcg gctactccct cgacctgccc agcgtcgccg agcgcctggc gcgcagcgac
     4501 cgcttcgacg aggtgtacta cgtggtgcgc gggccggacc gcgagcgcat cgccggcgac
     4561 gccgggctgc cgctcccgcc cgccggggcc gaacccgccg acgaggtgct ggcctacgat
     4621 gcggcgcgcg agggcaagcg gctgcgcctg gtggaagtgc gcgtgccctg cggcgggcgg
     4681 agctgcacgg tgaccatggc ggaaaccacg gtgaagcgcc agcgcctggc gcgcgacatc
     4741 ctcgcctcca gcgcgctgcc gctcgcgctg ctcgcggtgc tgacgctggc cctggtgtgg
     4801 ttcggcgtgg cgcgcggcct gcggccgctg caggggctct cggacgagat ccgcgcgcgc
     4861 tcgccgcacg acctgcgccc gctggacgcg acgcaggcgc ccgacgaggc gcggcccctg
     4921 gtcgagggca tcaaccggct gctgggcgag gtcgccgagg cgaaccgcaa ccagcagcgc
     4981 ttcctcgcca acgccgcgca ccagctgcgc acgccgctcg cggggctgca ggcgcacacc
     5041 gaactggcgc tcgcccagcc ggtgccgcag tcctgccgcg ccgagctcga gcaggtgcac
     5101 ggcgccaccg tgcgcacggc gcgcctcgcc aaccagctgc tggcgctggc gcgcgcggag
     5161 gcgctggcgc gcgcgcccga cgggcggccg ggcgccaatc tgcgcgcggt ggtcgaggag
     5221 tccgccgacg aatgggtgcg ccgcgccctg cagcgcgaca tcgacctggg cttcgagctg
     5281 gaggacctga cggtggcggg cgatgcgttc ctgctgcgcg aggcgctcgc caacctggtg
     5341 cacaacgccc tggagtacac ccccgcgggc ggccgcgtca cggtgcgcgc cgcgcgccgc
     5401 ggcgcgcagg gcgcggtgct cgaagtcgag gacgacggcc ccggcatcgc gccggcggag
     5461 cgcggccgcg tgctggagcg cttctaccgc gcccccggca ccggcggcac cggcagcggc
     5521 ctggggctgt cgatcgtgcg cgagatcgcc gccgcgcacg gcgcgctggt cgcgatcggc
     5581 gagggcgaag gcggccgcgg ctgccgcgtc acgctgcagt tcgcgggagt aaactagcgg
     5641 ccatggactc cgtggacatc gaagtgctgc gcagcgccga ggcctggcgc gccgccgggc
     5701 agcgcgtcac gctcggcacc atcgtcaaga cctggggctc ggcgccgcgg ccggtcggcg
     5761 cgctggtggt gatccgcggc gacggccagg tggtcggctc ggtgtcgggc ggctgcgtcg
     5821 aggacgacct gatcgacaag gtgcgctcgc aggccgtggc gaaggaccgg ccgcagctcc
     5881 tcacctacgg cgtgaccaac gaggaggcga cgcgctgggg actgccctgc ggcggcacgc
     5941 tgcagctggt gctcgagccg gtcggcgagg cgagcgcgct gggcgagctg ctgcgcacgg
     6001 tctcccgcca gcagctggtg aagcggcgcc tcgacatgca gagcggccgc gtcagcctcg
     6061 agcccgggca gtggcaggac gcgctcgagt tcgacggcgc caccctggtc acggtgcacg
     6121 ggccgcgctg gcgcctggtg ctgatcggcg ccgggcagct cacgcagtac ctggcggaga
     6181 tggcgcgcat gctcgactac cacgtcagcg tgatcgagcc gcgcgaggag tacgccagcg
     6241 gctggaacgt ggccggcgtc gagctggaac gccgcatgcc cgacgacgtc atcgccgcca
     6301 tgaatctcga cggccacagc gcggtggtgg cgctcacgca cgaccccaag atcgacgacc
     6361 tggcgctgat ggaggcgctg aaatccgccg cgttctacgt cggcgccatc ggctcgaaga
     6421 agaacaacga cgc
//
LOCUS       DASRIE010000104         9411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3502292, whole genome shotgun sequence.
ACCESSION   DASRIE010000104 DASRIE010000000
VERSION     DASRIE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502292"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..456
                     /locus_tag="VFB53_04665"
     CDS             97..456
                     /locus_tag="VFB53_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HZN86063.1"
                     /translation="MLGTLAGILVGLVALLHLYFLVLEMFLWTKPLGLKIFRNTPEKA
                     EASRVLAANQGLYNGFLAAGLAWGLWLGAAGRDIQLFFLVCVIAAGIYGAWSVSRRIL
                     WVQAAPAAVAMGLVLAA"
     gene            453..908
                     /locus_tag="VFB53_04670"
     CDS             453..908
                     /locus_tag="VFB53_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HZN86064.1"
                     /translation="MKDNVHPIGRGFCYEDLKVGFRFRTHRRSIAESDLASFVNLTWL
                     TEELFAVADDSDRAIKGRAVPGALVYSFAEGLLLPSMQDTGLAFLSATLDVKAPTVIG
                     DTIHVEVEVTECRLTSKGDRGLVRSRNQVVNQRGETVLEYNPLRMLKRK"
     gene            926..1315
                     /locus_tag="VFB53_04675"
     CDS             926..1315
                     /locus_tag="VFB53_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN86065.1"
                     /translation="MIDHVDHIVLTTRDLAGCIRFYAEVLGMKLESFRTPTETRQALK
                     FGNQKINLHEWGREFSPRAHVAAPGTLDLCFIAAIPLEGVIARLKAAQVPILEGPVMK
                     TGATGRIRSVYVRDPDLNLVEISEPAP"
     gene            complement(1312..2520)
                     /locus_tag="VFB53_04680"
     CDS             complement(1312..2520)
                     /locus_tag="VFB53_04680"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZN86066.1"
                     /translation="MGAVSLDEIRAAAVRIAGAVERTPCLHSRTLSRLAGCELWLKFE
                     NLQFTASFKERGALNKLLTLSDAERARGVIAMSAGNHAQGVAYHAARLGVRAVIVMPR
                     GTPNNKISSTRVHGAEVVLEGDTLAEAAAQARALASRERYTFIHPYDDDAVIAGQGTV
                     GLEMLEAVPDLEILVVPVGGGGLIAGIATAAKALRPGIRVLGVESKSWSAMHQRLNGL
                     PVTAGGDTIAEGMAVRDVGERTLAICRRTVDEVLLVEEEDIERAVVTLIEIEKTVAEG
                     AGAAGLAALLAFPQRFAGKRVGIPICGGNIDSRMLSAVLMRGLVRDGRLVRMRVAMPD
                     VSGNLARVAALIGEAGGNIVEVQHQRVFGTASVKTPQVEFVVETRDREHTRAIAAHLG
                     THGARAELDD"
     gene            2567..3073
                     /locus_tag="VFB53_04685"
     CDS             2567..3073
                     /locus_tag="VFB53_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86067.1"
                     /translation="MTELQRLLRALWALARASASPRWSRRVEGERVLAQVLAELRPAL
                     DAAGAQLSGGSLPVVRADPRLLARVLRALVGNAARHHGDEPPYIHVAARPAGREWLFS
                     VRDNGAGVDPRRHHAIFSAFSGATELPGCRRIIERHGGRMWVESARGHGATFYFSLPA
                     TREVGHDR"
     gene            3063..3872
                     /locus_tag="VFB53_04690"
     CDS             3063..3872
                     /locus_tag="VFB53_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN86068.1"
                     /translation="MIGRAMALALLLAPLAALGAELPVFDAHLHYSHDAWEVVPPADA
                     VAILKKAGVRRALVSSSGDDGQQRLKALAPELFVLELRPYRSRGVIGTWFRDESVPAY
                     LEERLQKFRYAGIGEFHLYGADADLPVPRRMVQLARQYGLHLHAHSDADAVERLFRQW
                     PQARVLWAHAGFEQPAKVREMLQKHRNLWCDLAFRTDHASGGRVDAEWRKAFLEFPDR
                     FLVGTDSFTPERWHYVAEHARWSREWLADLPAEVAERIAWKNGEALFAPGK"
     gene            4011..4730
                     /gene="phoB"
                     /locus_tag="VFB53_04695"
     CDS             4011..4730
                     /gene="phoB"
                     /locus_tag="VFB53_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZN86069.1"
                     /translation="MQASILVVDDDPAIRELLAANLAHSGYQVACAGSAAEAEAQVRE
                     LRPSVALLDWMLPGTSGLLLARQLRADPRTASVALIMLSARAEEQDKIAALESGADDY
                     VTKPFSTRELLARIKAVMRRRTPQLSEDVVEHAGLRLDPAARRASAGGRDLQLWSTEF
                     RILHFFMTHAGRVYARARLLDEIWGDHVFVEERTVDVHIRRLRQALAPSGHETLIETV
                     RGAGYRFRAETTEPLPRLRAA"
     gene            complement(4748..6175)
                     /locus_tag="VFB53_04700"
     CDS             complement(4748..6175)
                     /locus_tag="VFB53_04700"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HZN86070.1"
                     /translation="MLPAVVWPLTMLALHDARTQQLHRRLGPAALVRAGLDAAAAIGS
                     LLASVLWFGGRMDGACLILALLVFALTYPSNPARETADAKRLARDILAEWLAIAALLL
                     LLGWASRTLDAFDTRALSAWLLSAPLAMYAAHRLAPPLLARAFAAEGLHRTAVIAGGN
                     ELGRQLAQRIGASPYLGTQVAGFFDDRAGGRLEGIEPREQLGRLGDIAGYVKRHRVDV
                     IYIALPMASQPRILGLLEDLRDTTASIYFVPDILLFDLIQARIDSIGGMPVLAVCESP
                     FYGLNALVKRASDVALAAAILALIAPLMLAIAVGVKRSSPGPVLFRQRRYGLDGREIV
                     VYKFRSMTCTEDGVELRQAQRDDPRITPFGAFLRRYSLDELPQFVNVLQGRMSVVGPR
                     PHAVAHNEMYRRLIRGYMIRHKVKPGITGLAQVNGLRGETDTLDKMRARIECDLAYLR
                     GWSLLLDLQIVLKTVAVVLKGHNAY"
     gene            6304..7128
                     /locus_tag="VFB53_04705"
     CDS             6304..7128
                     /locus_tag="VFB53_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN86071.1"
                     /translation="MDLEHSATLQERRPHAASGAGIATLDSVQMETLTLNLDAALRVY
                     TRTQFFAWTQGLLQSLVPHDVLICAQGGAGAQALRVDSFSSLAPDAALFGELFQRDAT
                     AASLLKAWEDGGELPVSCRAQQGAALCGGLLGRELERLGATQLLAHGSRGADGHLAAF
                     FVFAGRPRRAGENDAYLLQLALPSLHFAWTRTRAEGGLAAGARAGAADAGVLTLRELE
                     ILRWIYLGKSNFEVGAILRISPLTVKNHVQKILRKLNVVNRTQAVGKALESRLLTP"
     gene            7325..8542
                     /locus_tag="VFB53_04710"
     CDS             7325..8542
                     /locus_tag="VFB53_04710"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN86072.1"
                     /translation="MAPRGQTGTGMPDCLGVCFLVLALLPTGEALALLDERLELSASQ
                     SAYRDDNVLRQPGGSPPSADTYRVTTLGLALDTPVGDQRLRGALAVNSVRYDSFGEFD
                     LDGHDGQGVWEWRAGDLKGKLGAAHRRALASLGSVQDGVQSRIPNALTTRRAYASGEA
                     MLGPSWALSLEGSRLEQSNAAEERRPNDLALDGGEAVLAYVSRAGNRLGLRGRAARGE
                     LPNPQLLGTAIDSSYRQRELAAVLDWRPGGHTRLRAHAGRVQRTYEQLPARDFSGGTW
                     ELLLDWAPGGKLEFGVVAQRAISDTEEINVSFVLAERVALQAKYRPSALTELSAVLET
                     ADRRYLGDAAQLLGAAPARSERLSAAGLQAGYRPLPQVTLSLALRREKRSDAVGIGAY
                     EVDVAGVGARIGF"
     gene            8693..9337
                     /locus_tag="VFB53_04715"
     CDS             8693..9337
                     /locus_tag="VFB53_04715"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HZN86073.1"
                     /translation="MSSARWKMAAGAAVLAATFGWAGIAQAQLGLPLPCLPGLCGGGG
                     GTATVVGQAAGVTATVLGNVVNLGDSGTVSDPSQPVGGGQPLGSIPGLLSAESLHSAA
                     MAWTDQVGSQASLSNLVLTVAGLGITADFLGSEALAIAGGALSGGSAIEGLTIGGVAV
                     PVTGLPNQQVSVPGLSVILNEQTQTANGIVVNALRVRTLDGLTDVALGSSRAGI"
BASE COUNT         1347 a         3391 c         3360 g         1313 t
ORIGIN      
        1 ctggcaggac atgatgcgcg gcgccgacca ggcgtggaaa gacatgcacg aggccttcaa
       61 ccgggccaag tcgcacttcg aaaagaagta ggcgcggtgc tcggcacgct ggcgggaatc
      121 cttgtggggc tggtggcgct actgcatctc tacttcctgg tgctggagat gttcctgtgg
      181 accaagcccc tcggcctcaa gatattccgc aacacgccgg agaaggccga ggccagccgg
      241 gtgctcgcag ccaaccaggg cctgtacaac ggcttcctgg ccgccggcct cgcctggggc
      301 ctgtggctgg gcgctgccgg ccgcgatatc cagctcttct tcctcgtctg cgtgatcgcc
      361 gccgggatct acggcgcctg gagcgtgagc cgacgcatcc tctgggtgca ggcggcgccc
      421 gcggcggtgg cgatggggct ggtgctggct gcatgaagga caacgtccac ccgatcggcc
      481 gcggcttctg ctacgaggat ctgaaggtcg gctttcgctt ccgcacccac cggcgcagca
      541 tcgcggagag cgacctggcg agtttcgtca acctcacctg gctcaccgag gagctgttcg
      601 cggtggccga cgattccgac cgcgccatca agggccgtgc ggtgccgggc gccctggtgt
      661 actccttcgc cgaggggctg ctgctgccct cgatgcagga caccggcctc gccttcctca
      721 gcgcgacgct ggacgtgaag gcgccgaccg tgatcggcga cacgatccac gtcgaagtcg
      781 aggtcaccga atgccgcctc acctcgaagg gcgaccgcgg cctggtgcgc tcgcgcaacc
      841 aggtggtgaa ccagcgcggc gagaccgtgc tcgagtacaa cccgctgcgc atgctcaagc
      901 gaaaataggg tcagacccca ttttcatgat cgaccacgtc gaccacatcg tcctcaccac
      961 gcgcgacctg gcgggctgca tccgcttcta tgcggaggtg ctggggatga agctggagtc
     1021 gttcaggacg cccaccgaga cgcgccaggc gctcaagttc ggcaaccaga agatcaacct
     1081 gcacgagtgg ggcagggagt tcagcccgcg cgcgcacgtc gccgcgccgg gaacgctcga
     1141 cctgtgcttc atcgccgcga ttcccctgga gggggtgatc gcgaggctga aggcggcgca
     1201 agtgccgatt ctcgaaggtc cggtgatgaa gaccggcgcc accggcagga tccgctcggt
     1261 gtacgtgcgc gacccggacc tgaacctggt cgagatctcc gagccggcgc cctagtcgtc
     1321 cagctccgcg cgcgccccgt gcgtgcccag gtgcgcggcg atggcgcgcg tgtgctcgcg
     1381 gtcgcgcgtc tccaccacga attccacctg cggcgtcttc accgacgcgg tgccgaacac
     1441 gcgctggtgc tgcacttcga cgatgttgcc gcccgcctcg ccgatcagcg cggccacgcg
     1501 cgcgaggttg cccgagacgt cgggcatcgc cacgcgcatg cgcaccaggc gcccgtcgcg
     1561 caccagcccg cgcatcagca ccgccgagag catgcgcgag tcgatgttgc cgccgcagat
     1621 cgggatgccg acccgcttgc cggcgaagcg ctgcgggaag gcgagcaggg cggcgagccc
     1681 cgccgccccc gctccctcgg cgaccgtctt ctcgatctcg atcagcgtca ccaccgcgcg
     1741 ctcgatgtct tcctcctcga ccagcagcac ctcgtccacc gtgcgacggc agatggcgag
     1801 ggtgcgctcg cccacgtcgc gcaccgccat cccctcggcg atggtgtccc cgcccgccgt
     1861 caccggcagg ccgttgaggc gctggtgcat ggcgctccag gacttcgatt ccacgcccag
     1921 cacccggatc ccggggcgca gcgccttcgc ggcggtggcg atgcccgcga tcaggccgcc
     1981 gccgcccacc ggcaccacca ggatctccag gtccggcacc gcctcgagca tttccagccc
     2041 caccgtgccc tggccggcga tcaccgcatc gtcgtcgtag ggatggatga acgtatagcg
     2101 ctcgcgcgag gccagcgcac gcgcctgcgc cgccgcctcg gccagcgtgt ctccctcgag
     2161 gaccacctcg gcgccgtgca cgcgcgtgct gctgatcttg ttgttgggcg tgccgcgcgg
     2221 catcacgatc accgcgcgca cgcccaggcg cgcggcgtga taggccacgc cctgcgcatg
     2281 gttgccggcg ctcatggcga tgacgccgcg cgcgcgctcc gcgtcgctca gcgtgagcag
     2341 cttgttgagc gcgccgcgct ccttgaacga ggcggtgaac tgcaggttct cgaatttcag
     2401 ccacagctcg cagccggcga gccgcgacag cgtgcggctg tgcaggcagg gcgtgcgctc
     2461 caccgcgccg gcgatgcgca ccgccgccgc gcggatttca tcgagtgaaa cggcgcccat
     2521 gcccgagtct agcccaggcc atagaatcgc ggcatggaac ggacccttga ctgaactgca
     2581 gcggctgctg cgcgcgctgt gggcgctcgc ccgcgcgagc gcgtcgccgc gctggtcgcg
     2641 gcgcgtggag ggcgagcgcg tgctggcgca ggtgctcgcc gagctgcgcc cggcgctcga
     2701 tgccgcgggc gcccagcttt cgggcggctc gctgccggtg gtgcgcgccg acccgcgcct
     2761 gctggcgcgg gtgctgcgcg ccctggtcgg caacgccgcg cgccaccacg gcgacgaacc
     2821 gccctacatc cacgtcgccg cgcgcccggc cgggcgcgag tggctgttct cggtgcgcga
     2881 caacggcgcg ggggtggatc cccggcgcca ccacgccatc ttcagcgcgt tttccggcgc
     2941 caccgagctg cccggctgcc gccgcatcat cgagaggcac ggcggccgga tgtgggtaga
     3001 atcggcgcgc gggcacgggg cgaccttcta cttcagcctg cccgcgacgc gggaggtggg
     3061 gcatgatcgg tagggcgatg gcgctggcgc tgctcctcgc gccgcttgcg gcgctcggcg
     3121 ccgagctgcc ggtgttcgac gcccacctgc actacagcca cgacgcctgg gaggtggtgc
     3181 cgcccgcgga cgcggtggcg atcctgaaga aggccggcgt gcgccgcgcg ctggtgtcct
     3241 cctcgggcga cgacggccag cagcggctga aggcgctggc gccggaattg ttcgtcctcg
     3301 agctgcgtcc ctaccgctcg cgcggcgtga tcggcacctg gttccgcgac gagtcggtcc
     3361 cggcctacct ggaagaacgc ctgcagaaat tccgctatgc cggcatcggc gaattccacc
     3421 tgtacggcgc cgacgccgac ctgccggtgc cgcggcgcat ggtgcagctg gcgaggcagt
     3481 acggcctgca cctgcacgcg cattcggacg ccgacgcggt ggagcggctg ttccgccagt
     3541 ggccgcaggc gcgcgtcctc tgggcgcacg cgggcttcga gcagccggcg aaggtacgcg
     3601 agatgctgca gaagcaccgc aacctgtggt gcgacctcgc cttccgcacc gaccacgcga
     3661 gcggcggcag ggtggacgcg gagtggcgca aggcgttcct cgagtttccg gaccgcttcc
     3721 tggtcggcac cgacagcttc acccccgagc gctggcacta cgtcgccgag catgcacgct
     3781 ggtcgcgcga gtggctcgcc gacctgcccg cggaggtcgc cgagcgcatc gcctggaaga
     3841 acggcgaggc attgttcgcg ccgggcaagt gagacttccg cccgtcctgt agtcggcgcg
     3901 ctacaacgcg ccgtggcccc accacagcgc aatgcccccg cgggcgatag ctgccggccc
     3961 ggccgccccc tagcatcccc ttcgtctatc caacaggggg tgcccagatc atgcaagctt
     4021 cgatcctggt agtcgacgac gacccggcga ttcgcgagtt gcttgcggcc aacctggcgc
     4081 attccggtta ccaggtggcc tgcgcgggca gcgccgccga ggccgaggcg caggtgcgcg
     4141 aacttcgccc gagcgtggcg ctgctcgact ggatgctgcc ggggacctcc ggcctgctgc
     4201 tggcgcgcca gctgcgcgcc gacccgcgca ccgccagcgt ggcgctgatc atgctgagcg
     4261 cgcgcgccga ggagcaggac aagatcgccg cgctggagag cggcgccgac gactacgtga
     4321 ccaagccgtt ctccacgcgc gagctgctgg cgcgcatcaa ggcggtgatg cgccggcgca
     4381 cgccgcagct ctcggaagac gtggtggagc acgcgggcct gcgcctggat cccgcggcgc
     4441 gccgcgccag cgccggcggg cgcgacctgc agctgtggag caccgagttc cgcatcctgc
     4501 acttcttcat gacccacgcc gggcgcgtct acgcgcgcgc gcgcctgctc gacgagatct
     4561 ggggcgacca cgtgttcgtc gaggagcgca cggtcgacgt gcatatccgg cgcctgcgcc
     4621 aggcgctcgc gcccagcggg cacgagacgc tcatcgagac ggtgcgcggc gcgggctacc
     4681 gcttccgcgc cgagacgacc gagccgctgc cgcgcctgag ggcggcgtga gccctgggca
     4741 gccgcgctca gtacgcgttg tgccccttca gcaccacggc caccgtcttc agcacgatct
     4801 gcaggtcgag gagcagcgac cagccgcgca ggtaggcgag gtcgcattcg atgcgcgcgc
     4861 gcatcttgtc cagcgtgtcg gtctcgccgc gcaggccgtt gacctgcgcc aggccggtga
     4921 tgccgggctt caccttgtgc cggatcatgt agccgcggat caggcggcgg tacatctcgt
     4981 tgtgcgcgac cgcgtgcggg cgcggcccga ccacgctcat gcgcccctgc agcacgttga
     5041 cgaactgcgg cagctcgtcg agcgagtagc gccgcaggaa ggcgccgaac ggcgtgatgc
     5101 gcgggtcgtc gcgctgcgcc tggcgcagtt ccacgccgtc ctccgtgcag gtcatggagc
     5161 ggaacttgta gaccacgatc tcgcgcccgt ccaggccgta gcgccgctgg cggaacagca
     5221 ccgggcccgg cgagctgcgc ttcacgccga cggcgatggc gagcatcagc ggcgcgatga
     5281 gcgcgaggat cgcggcggca agcgcgacat cgctggcgcg cttcaccagc gcgttcaggc
     5341 cgtagaacgg cgactcgcac accgccagca ccggcatgcc gccgatgctg tcgatgcgcg
     5401 cctggatcag gtcgaacagc aggatgtcgg gcacgaagta gatcgaggcg gtggtgtcgc
     5461 gcaggtcctc cagcaggccg aggatgcgcg gctgcgacgc catcggcagc gcgatgtaga
     5521 tcacgtccac gcggtggcgc ttgacgtagc cggcgatgtc gcccaggcgg ccgagttgct
     5581 cgcggggttc gatgccctcc agccgcccgc cggcgcggtc gtcgaagaag ccggcgacct
     5641 gcgtgccgag gtaggggctc gcgccgatgc gctgcgccag ctgccggccc agctcgttcc
     5701 cgccggcgat caccgccgtg cggtgcaggc cctcggcggc gaaagcgcgc gccagcaggg
     5761 gcggggcgag gcggtgcgcg gcgtacatgg ccagcggcgc cgagagcagc caggcggaaa
     5821 gcgcgcgcgt gtcgaatgcg tccagcgtgc gcgaagccca gccgagcagc agcagcagcg
     5881 cggcgatcgc cagccattcg gccaggatgt cgcgcgccag gcgcttcgcg tcggcggtct
     5941 cgcgcgccgg attggacgga taggtgagcg cgaacaccag cagcgcgagg atcaggcagg
     6001 cgccgtccat gcggccgccg aaccacagca cgctggcgag caggctgccg atcgccgcgg
     6061 cggcatcgag ccccgcgcgc accagggcgg cgggccccag ccgtcggtgc agttgctgcg
     6121 tgcgggcgtc gtgcagggcg agcatggtca gcggccagac tacggcaggc agcatgctgc
     6181 gcggatgatc cctcgccccc gccgcggtag ctcgttcggc ctagtgccgc gtccgccggc
     6241 agatccgtgt cacggctgcg tcatattgca tcctctatcc tgaccacggc gaacgcagct
     6301 gccatggacc tcgagcacag tgccaccctg caggagcgca ggccccacgc ggcctcgggc
     6361 gcgggcatcg cgacgctgga cagcgtgcag atggaaacgc tgacgctcaa cctggacgcc
     6421 gcgctgcggg tgtacacgcg cacgcagttc ttcgcctgga cgcaggggct gctgcaaagc
     6481 ctggtgccgc acgacgtgct gatctgcgcg cagggcggcg cgggcgcgca ggcgctgcgc
     6541 gtcgacagct tctccagcct ggcgccggac gcggcgctgt tcggcgagct gttccagcgc
     6601 gacgccaccg ccgccagcct gctgaaggcc tgggaggacg gcggcgagct gccggtcagc
     6661 tgccgcgcgc agcagggcgc cgcgctgtgc ggcgggctgc tcgggcgcga gctcgagcgg
     6721 ctgggcgcca cccagctcct cgcgcacggc agccgcggcg cagatgggca tttggcggcg
     6781 ttcttcgtgt tcgccggccg gccgcgccgc gcgggggaga acgacgccta cctgctccag
     6841 ctcgccctgc cgtcgctgca cttcgcctgg acgcgcacgc gcgccgaggg cggcctggcg
     6901 gcgggcgccc gcgccggcgc cgccgacgcg ggcgtgctca cgctgcgcga gctggaaatc
     6961 ctgcgctgga tctatctcgg caagagcaac ttcgaggtcg gcgccatcct gcgcatcagc
     7021 ccgctcaccg tgaagaacca cgtgcagaag atcctgcgca agctgaacgt ggtgaaccgc
     7081 acccaggcag tcggcaaggc gctggagtcg cgcctgctga cgccttgagc catagtccga
     7141 ccggaccatg gcacggtcac tgcggagcat gtgcccggcg gcggcgctgg cctaagcttc
     7201 catccagcac gcccaaaacc ctcgggcagc ggtaaggcac tacgctccgc gcgcaggcag
     7261 catgggacgc ccacccccca taccccctga ggcggtccag ccggaatttc ggcgcctgcg
     7321 cagcgtggcc ccgagggggc agaccggcac cggaatgccg gactgcctgg gcgtgtgttt
     7381 ccttgtgctc gcgttgctgc cgacgggcga agcgctcgcc ctgctggacg agcggctgga
     7441 gctctcggcg tcccaatcgg cgtaccgcga cgacaacgtg ctgcgccagc ccggcggcag
     7501 cccgccgagc gccgacacct accgcgtcac caccctcggc ctcgccctcg acacgccggt
     7561 cggcgaccag cgcctgcgcg gcgcgctggc ggtgaacagc gtgcgctacg acagcttcgg
     7621 cgagttcgac ctggacgggc acgacggcca gggcgtctgg gaatggcgcg cgggcgactt
     7681 gaagggcaag ctcggcgccg cgcaccgccg cgcgctcgcc tcgctcggca gcgtgcagga
     7741 cggcgtgcaa agccgcatcc cgaacgcgct caccacgcgc cgcgcctacg cctcgggcga
     7801 agccatgctc gggccgagct gggcgctctc cctggagggc tcgaggctgg agcagtcgaa
     7861 cgcggccgag gagcgccggc cgaacgacct cgcgctcgac ggcggcgagg cggtcctcgc
     7921 ctacgtcagc cgcgccggca accggctcgg cctgcgcggc cgcgctgcgc gcggcgaact
     7981 gccgaatccg caattgctcg ggacggcgat cgacagctcc tatcgccagc gcgagctcgc
     8041 ggcggtgctc gactggcggc cgggcggcca cacgcgcctg cgcgcgcacg ccgggcgcgt
     8101 gcagcggacc tacgagcagc tgccggcgcg cgatttctcc ggcggcacct gggaactgct
     8161 gctcgactgg gcgccgggcg gcaaactgga gttcggcgtt gtcgcgcagc gcgccatcag
     8221 cgacaccgaa gagatcaacg tcagcttcgt gctcgccgag cgcgtggcgc tgcaggcgaa
     8281 ataccggccg agcgccctca ccgagctgag cgcggtgctc gagaccgccg accggcgcta
     8341 cctgggcgac gcggcgcagc tgctcggcgc ggcgccggcg cgcagcgagc ggctgagcgc
     8401 cgccgggctg caggcgggct accggccgct gccgcaggtc acgctgagcc tggcgctgcg
     8461 gcgcgaaaag cgctcggatg cggtgggcat cggcgcgtac gaagtcgacg tcgccggcgt
     8521 cggggcgcgc atcggcttct agatgacacg cggctgcgcc gaacggcgtg gcccgctagg
     8581 actacggcat ggtcccggta gcgcatgccc atcgccctgc ggcgtcacgc cgcgcgccta
     8641 acttgtcccg cacacgccgc attcgcggcg gttgcgatga aaggagaagc acatgagtag
     8701 cgcacgatgg aaaatggccg cgggcgcggc ggttctcgcc gcgaccttcg gctgggcggg
     8761 aatcgcccag gcgcagttgg gcctgccgtt gccctgcctg ccgggattgt gcggaggcgg
     8821 aggcggtacg gccacggtag tcggccaggc cgccggcgtg accgccacgg tactgggcaa
     8881 cgtagtgaac ctcggcgaca gcggcacggt gagcgacccg tcccagccgg tcggcggggg
     8941 ccagcccttg ggcagcatcc ccggcctgct cagcgccgag tcgctgcatt cagcggcgat
     9001 ggcctggacc gaccaggtgg gctcgcaggc ctcgctcagc aatctcgtcc tgacggtcgc
     9061 cggcctgggc atcacggccg acttcctcgg gtccgaggcg ctggcgatcg ccggtggagc
     9121 cctcagcggc gggagcgcca tcgaggggtt gacgatcggc ggggttgcgg ttcccgttac
     9181 cggattgccc aaccagcagg tctcggtgcc gggcctgagc gtgatcctca acgagcagac
     9241 gcagaccgcc aacggcatcg tcgtcaacgc gctgcgcgtg cgcacgctcg acggcctcac
     9301 cgacgtggcc ctcggctcgt cgcgcgccgg aatctgaaag gagccatgac catgaaacac
     9361 acactaagac tcctctgcgc gcttcccttc gccttcctcg ccggccaggc g
//
LOCUS       DASRIE010000105         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3231652, whole genome shotgun sequence.
ACCESSION   DASRIE010000105 DASRIE010000000
VERSION     DASRIE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231652"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VFB53_04720"
     CDS             <1..1568
                     /locus_tag="VFB53_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86074.1"
                     /translation="VAQVGACIGRQFSRDLLANVLALDAAALNAALEQLEGAGLLVRT
                     GTPASAIYAFKHAMVQEVAYNSLLRTRRKHFHAEIAAALAKSADAGSAPEVLAHHYME
                     AGQFEHAAACWWQAAERAGARYANQEAIAHCRKGLTALSHLPRDRKRIDMELALRIGL
                     ASGLRLNDHHSEALAELGTADVVAAESGRVVELSRIHHLRGNIYYPLGKIENCFGEHE
                     AALRFAKQAGSTEDEARALGGVGDAHFLAGRIQEAHRHFEDCVALARQNGFLLTQVAY
                     LPMRAVTHMYCLRFNESLNDCLSVMDLVMRVGQARGELISLSTSSWILLDQCDFRRAE
                     EHARRGLDVVQAIGARRFIPLFNDVLARIRLQAGDRTGALALLDESWKASRESGVTFA
                     GPVVLGAVALATTDPGRRFEVLHQGEAILRDGCPSHNHFRFYRDAIELSLREQQWDTA
                     EAYAGAFEGYFAGKGSPWSDFFIARGRALAAAGRARSRRAMAPQLRELRDYAGMHGIL
                     AAVPRLDAALSDA"
     gene            1669..>2060
                     /locus_tag="VFB53_04725"
     CDS             1669..>2060
                     /locus_tag="VFB53_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZN86075.1"
                     /translation="MAKAVEGVAGRILPALALLAALAVAPAAALDPRYVDADGDLVAD
                     TPARTVDPPTLIFAYTPGEDPALYPRVWDGFMRHLERVTGKPLKLFPAPSNTAQIEAM
                     RSGRLHIAAFGTGAVPVAVNCAGFVPMA"
BASE COUNT          304 a          716 c          718 g          322 t
ORIGIN      
        1 aggtcgcgca ggtcggcgcc tgcatcggcc gccagttttc gcgcgacctg ctcgccaacg
       61 tgctggcgct cgacgcggcc gcgttgaacg ccgcgctcga acagttggag ggcgccgggc
      121 tgctggtgcg caccggcacg ccggccagcg ccatctatgc gttcaagcac gcgatggtgc
      181 aggaggtcgc atacaacagt ctcctcagga cgcggcgcaa gcatttccac gccgagatcg
      241 ccgcggcgct cgccaaatcc gccgacgccg gctcggcgcc ggaggtgctc gcgcatcact
      301 acatggaggc gggccagttc gagcacgcgg cggcgtgctg gtggcaggcc gccgagcgcg
      361 ccggcgcgcg ctacgccaac caggaagcga tcgcgcattg ccggaagggt ttgacggcgc
      421 tctcgcacct gccgcgcgat cgcaagcgta tcgacatgga gcttgcgctg cgtatcggcc
      481 tcgcctcggg gctgcgcctc aacgaccatc atagcgaggc gctggcggaa ctggggacgg
      541 ccgacgtggt cgcggccgag agcgggcgcg ttgtggaact gtcgcgcatc catcacctgc
      601 gtggcaacat ctactacccg ctggggaaga tcgagaattg cttcggcgag cacgaggccg
      661 cgctgcgctt cgccaagcag gccggctcca ccgaagacga ggcgcgcgcg ctcggcggcg
      721 tgggcgatgc ccacttcctg gccgggcgca tccaggaggc gcaccggcac ttcgaggact
      781 gcgtcgcgct cgcgcgccag aacgggttcc tcttgaccca ggtggcctat ctgccgatgc
      841 gcgccgtcac ccacatgtac tgcctgcgct tcaatgagtc gctcaacgac tgcctctcgg
      901 tcatggacct ggtgatgcgg gtcggccagg cgcgcggcga gctcatctcg ctgagtacca
      961 gcagctggat cctgctcgat caatgcgact tccggcgcgc cgaggagcac gcccgcaggg
     1021 gcctcgacgt ggtgcaggcg atcggtgcac gccgtttcat cccgctgttc aacgacgtcc
     1081 tcgcgcgcat ccggctccag gccggcgatc gcaccggcgc gctcgcgctg ctggacgaaa
     1141 gttggaaagc ctcgcgggaa tccggggtga cgttcgccgg tccagtggtg ctcggcgccg
     1201 tggcgctcgc cacgaccgac cccgggcgcc gctttgaagt gctgcatcag ggcgaggcga
     1261 tcctgcggga cggatgtccg agccacaacc atttccgctt ctaccgcgac gccatcgagc
     1321 tcagcctgcg cgagcagcag tgggacacgg cggaagccta cgccggcgct ttcgaaggct
     1381 atttcgcggg caagggttcc ccatggtcgg atttcttcat cgcgcggggg cgcgcccttg
     1441 cggcagccgg gcgcgcgcgt tcgcgccggg caatggcgcc gcaactgcgg gagctgcgcg
     1501 actatgccgg catgcacggc atcctggcgg cggtgccgcg tcttgacgca gccctctccg
     1561 acgcttgacg cagtcgtaaa ctagcacgag gaatgtcagg ctacggccgc gacggggtga
     1621 ctctccgcgg cccggcgcga gcgggcgcgc attggggagc gcaccacaat ggcgaaggca
     1681 gtcgagggcg ttgcgggtcg tattctgccg gcgctggcat tgctcgccgc gctcgcggtc
     1741 gcgccggcag ccgcgcttga tccgcgctac gtcgatgccg acggcgacct cgtggcagat
     1801 accccggccc ggacggtcga cccgcccacc ctcatcttcg cctatacgcc gggtgaagac
     1861 ccggcgcttt acccgcgcgt atgggacggc ttcatgcgcc atctggaacg cgtgaccgga
     1921 aagccgctca agctgtttcc ggcgccctcc aacacggcgc agatcgaggc gatgcgctcc
     1981 ggccggctgc acatcgcggc cttcggcacc ggcgcggtgc cggtcgccgt gaactgcgcc
     2041 ggcttcgtgc cgatggcgat
//
LOCUS       DASRIE010000106         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2190679, whole genome shotgun sequence.
ACCESSION   DASRIE010000106 DASRIE010000000
VERSION     DASRIE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190679"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFB53_04730"
     CDS             <1..612
                     /locus_tag="VFB53_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN86076.1"
                     /translation="VLQIPVNEKKGLTFAKGLRSILRHDPDKIMVGEIRDPETANIAV
                     QSALTGHLVFTTVHANNVFDVIGRFMHMNVDLHSFVSALNAIVAQRLVRLSCTHCAEP
                     YAPDDELLQESGVADTRGYRFLKGKGCRECRGLGYRGRKAIAELLLMNDEMRELIIAR
                     APARSLKTAAAANGTRFLRQAALDSVKAGETTLEEINRVTFVA"
     gene            593..1234
                     /locus_tag="VFB53_04735"
     CDS             593..1234
                     /locus_tag="VFB53_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86077.1"
                     /translation="MSLLSRDRLLIALEPHAVHAVRPKTNEKRSASGAWPEALKQALA
                     GWTSTAATVVVSNRLVRYVVVPRTAGVSGDAEETALARHHFTRIYGERARDWDVRYSR
                     ETGLASAMDMELIDELRKALKLRSLQPYLMAAFNRLRSKVPAAGAWIVLPEAEATCVA
                     LFTKGAWAGVSVSRSAGDEAIARERLRMGGAQAPETVLTPPANDGYAMAMSAA"
     gene            1231..1740
                     /locus_tag="VFB53_04740"
     CDS             1231..1740
                     /locus_tag="VFB53_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86078.1"
                     /translation="MRPPRLELDHAAPRRRPRWPGFALLGLSLAIAAHLGSSYRDTAS
                     ALRNVPTAAPAPAPRPAPSKTADAEEKVARAAMRQLALPWAGLIRALEDSATPDVALL
                     QLQPEAQQQVLRVTAEARSAAAMFRYLKGLSGAKGLSEIHLVTHEVAQDDPQRPVRFA
                     AQASFRGME"
     gene            1737..2246
                     /locus_tag="VFB53_04745"
     CDS             1737..2246
                     /locus_tag="VFB53_04745"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HZN86079.1"
                     /translation="MRHFAERLGVAGVLGIGVLLFCATFYVSTLRPAQQLAARQSAAA
                     GTSTVQVQAPLARTGDPLAELHSRFPALDALPSQVERLHRIARSSGLQLQTGEYRLEV
                     PPAGLVAYRVALPARGEYRALRTFIGAVLKEMPVAAIDRVRFERKKPGDAQLEAHLQL
                     TLYFRPEKP"
     gene            2243..4096
                     /locus_tag="VFB53_04750"
     CDS             2243..4096
                     /locus_tag="VFB53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZN86080.1"
                     /translation="MRLPAFILVLLLAACATEPEKAPPQLIPEPTVEAAAKKMRENPG
                     DLVARAEYFRTRDLAIAQWLAQADSLRAARQYEQAAELYRRVERHESGNARAAAGLEN
                     LVTDRRHDGVLAEADKLVQAERYGEAQETLRQVLAENPRHADARRLQRLIEERTVKAA
                     LPRLRSAKPMSLDLREVPVRSVFDVIGRTAGYNFVFDRDVRPDLRTSVTVRELAVEEL
                     ISLVLTTNQLDQRVINDSTVLVYPNTPAKQREYQELVVKVFYVGNADSKQTANMLRLM
                     LKMRDVFVDEKLGMVVIKDTPAMVRLAERLIAAQDLAEPEVMLEVEVLEIGSNRLVEL
                     GLRFPDSLAVGLVGAGGPGTLTLQEWRTGGSELVRFTFTDPLFLFLLRQQDGSTKVLA
                     NPRIRVKNKEKARVHIGDRVPVITTTAAATGGFVSESVNYLDVGLKLEVEPQIHLEDE
                     VGIKVGLEVSNIAREIRTVGSNTLTYQIGTRNAATVLRLRDGETQILAGLISDEDRRS
                     ASRVPGLGDLPVIGRLFSQTRENITKTEIVLLITPRVVRTLARPEVRAIEFAAGTEAS
                     GGASAFRGSAPPPPQVPQIQPAPQPEKPPAPPPGQPQGNTMVPFGGVQPPR"
     gene            4099..4554
                     /locus_tag="VFB53_04755"
     CDS             4099..4554
                     /locus_tag="VFB53_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZN86081.1"
                     /translation="MRRARGFTLIELLITVAIVAILASAARPLSELNVKRAKELELRR
                     ALREIRDGLDAYRQAAEDGRIERRVGDSGYPRRLEDLFEGVDDRRAPNKQRIYFLRRL
                     PRDPMTPDGQWGLRSYASPPDSPAPGDDVFDVYTLSSGVGINGRPYREW"
     gene            4562..4933
                     /locus_tag="VFB53_04760"
     CDS             4562..4933
                     /locus_tag="VFB53_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZN86082.1"
                     /translation="MRLRGFTLIELLVVMAIVATLLTLAVPRYFVSIDRSREAVLKEN
                     LFQIRDAIGKYHADKGRYPESLQVLATEQYLRKVPIDPVTQSEMTWVVVPVPGTETAG
                     VFDVRSGAQGKSTDGSDFATW"
BASE COUNT          794 a         1811 c         1748 g          685 t
ORIGIN      
        1 gtgctgcaga tcccggtgaa cgagaagaag ggcctcacct tcgccaaggg gctgcgctcg
       61 atcctgcgcc acgacccgga caagatcatg gtgggcgaga tccgcgaccc ggagaccgcc
      121 aacatcgcgg tgcagtcggc gctcaccggc cacctggtat tcaccacggt gcacgcgaac
      181 aacgtgttcg acgtcatcgg ccgcttcatg cacatgaacg tcgacctgca cagcttcgtc
      241 tcggcgctga acgcgatcgt ggcgcagcgc ctggtgcgcc tcagctgcac gcactgcgcc
      301 gagccgtatg cgccggatga cgagctgctg caggaatccg gcgtcgccga tacccggggc
      361 tatcgattcc tgaaaggcaa gggctgccgc gagtgccgcg gcctgggcta ccgcgggcgc
      421 aaggcgatcg cggagctgct gctgatgaac gacgagatgc gcgagctgat catcgcgcgc
      481 gccccggcgc gcagcctgaa gaccgcggcg gcggcgaacg gcacgcgctt cctgcgccag
      541 gcggcgctcg attcggtcaa ggccggcgaa accaccctgg aggagatcaa ccgtgtcact
      601 tttgtcgcgt gaccggctcc tgatcgctct ggagccgcac gcggtgcacg cggtgcggcc
      661 gaagaccaat gagaagcgca gcgcgagcgg cgcctggccc gaggcgctga aacaggcgct
      721 cgccggctgg acgagcaccg cagccacggt cgtggtttcc aaccgcctgg tgcgctacgt
      781 ggtggtgccg cgcaccgcag gcgtaagcgg cgacgcggag gagaccgcgc tcgcgcgcca
      841 ccacttcacg cgcatctacg gcgaacgcgc ccgcgactgg gacgtgcgct acagccggga
      901 aaccggcctt gccagcgcca tggacatgga gctgatcgac gagcttcgca aggcgctgaa
      961 gctgagatcg ctgcagccct atctgatggc ggccttcaac cggctgcgtt cgaaggtgcc
     1021 cgccgcgggt gcctggatcg tgctgccgga agcggaagcc acctgcgttg cattgtttac
     1081 caagggcgcc tgggccggcg tttcggtcag ccgctcggcc ggtgacgaag cgattgcacg
     1141 tgagcgtctc cggatgggcg gcgcccaggc gccggagacc gtgcttacac cgccagcgaa
     1201 cgatggctac gccatggcga tgagcgccgc atgagaccgc cgcgcctgga gctcgatcac
     1261 gccgcgccgc gccggcggcc gcgctggccc ggcttcgccc tcctcggcct ttcgctcgcc
     1321 atcgccgcgc atctcggatc gagctaccga gacacggcga gcgcgctgcg caacgttccg
     1381 acggcggcgc cggcgccggc gccgcgcccc gccccgagca agaccgccga cgcggaagag
     1441 aaagtggcgc gcgcagcgat gcgccagctc gcgctgccgt gggccggcct gatccgcgcg
     1501 ctggaggatt ccgccacccc cgacgtcgcc ctgctccagc tccagcccga ggcccagcag
     1561 caggtgctgc gcgtgaccgc ggaggcgcgc agcgcggcgg cgatgttccg ctacctgaag
     1621 ggcctgtcgg gcgccaaggg actgtcggag atccacctgg tgacgcacga ggtggcgcag
     1681 gacgatccgc agcgcccggt gcgcttcgcc gcgcaggcct cgttccgggg gatggaatga
     1741 ggcacttcgc cgagcggctt ggcgtcgccg gcgtgctcgg catcggcgtg ctgctgttct
     1801 gcgcgacctt ctacgtctcc accctgcgcc cggcgcagca gctcgccgcg cgccagtcgg
     1861 ccgcagcggg caccagcaca gtccaggttc aagcgccgct cgcccgcacc ggcgacccgc
     1921 tcgccgagct gcattcgcgc ttcccggcgc tcgacgccct gccgtcgcag gtcgagcgcc
     1981 tgcaccgcat cgcgcgctcc tcggggctgc agctgcagac cggcgagtac cggctggaag
     2041 tgccgcccgc ggggctggtc gcctaccgcg tggcgctgcc ggcgcgcggc gaataccgcg
     2101 cgctgcgcac cttcatcggc gcggtgctca aggaaatgcc ggtcgccgcc atcgaccgcg
     2161 tgcgcttcga gcgcaagaag ccgggcgatg cccagctcga ggcgcacctt cagttaacgc
     2221 tgtacttccg gccggagaaa ccatgaggtt gcccgcattc atccttgtcc tgctgctcgc
     2281 cgcgtgcgcc accgagcctg aaaaggcgcc gccgcagctg attcccgagc cgacggtcga
     2341 agcggcggct aagaaaatgc gcgagaaccc gggcgacctg gtggcgcgcg ccgagtactt
     2401 ccgcacccgc gacctggcga tcgcgcagtg gctcgcccag gccgacagcc tgcgcgccgc
     2461 gcgccagtac gagcaggcgg cggagctcta ccggcgcgtc gagcgccacg aatcgggcaa
     2521 cgcgcgcgcc gccgccggcc tcgagaacct cgtcaccgac cggcggcacg acggcgtgct
     2581 ggcggaagcg gacaagctgg tgcaggccga gcgctatggc gaggcgcagg agacgctgcg
     2641 ccaggtgctg gcggagaatc cgcgccacgc ggacgcgcgg cgcctgcagc gcctgatcga
     2701 ggagcgcacg gtgaaggccg cgctgccgcg cctgcgcagc gccaagccga tgtcgctcga
     2761 cctgcgcgag gtgccggtgc gctcggtgtt cgacgtgatc gggcgcaccg ccggctacaa
     2821 cttcgtgttc gaccgcgacg tgcgcccgga cctgcgcacc agcgtcacgg tgcgcgagct
     2881 cgcggtcgag gagctgatca gcctggtgct caccaccaac cagctcgatc agcgcgtgat
     2941 caacgacagc acggtgctgg tctacccgaa tacgccggcc aagcagcgcg agtaccagga
     3001 gctggtggtg aaggtgttct acgtgggcaa cgccgattcc aagcagaccg ccaacatgct
     3061 gcgcctgatg ctcaagatgc gcgacgtgtt cgtcgacgag aagctcggca tggtggtgat
     3121 caaggacacg ccggctatgg tgcgcctcgc cgagcgcctg atcgcggcgc aggacctggc
     3181 cgagccggag gtgatgctgg aagtcgaggt gctggagatc ggcagcaacc gcctggtcga
     3241 gctcggcctg cgcttccccg actcgctcgc ggtgggcctg gtcggcgccg gcggccccgg
     3301 caccctcacg cttcaggagt ggcgcaccgg cggctccgag ctggtgcgct tcaccttcac
     3361 cgatccgctg ttcctcttcc tgctgcgcca gcaggacggc tcgaccaagg tgctcgccaa
     3421 cccgcgcatc cgggtgaaga acaaggaaaa ggcgcgcgtg cacatcggcg accgcgtgcc
     3481 ggtgatcacc accaccgcgg ccgccaccgg cggcttcgtc tcggagtcgg tgaactacct
     3541 ggatgtgggc ctgaagctcg aggtcgagcc gcagatccac ctcgaggacg aggtcggcat
     3601 caaggtcggg ctcgaggtga gcaacatcgc gcgcgagatc cgcaccgtgg gcagcaacac
     3661 gctcacctac cagatcggca cgcgcaacgc cgccaccgtg ctgcgcctgc gcgacggcga
     3721 gacccagatc ctcgccggcc tgatcagcga cgaagaccgg cgcagcgcga gccgcgttcc
     3781 cgggctcggc gacctgccgg tgatcgggcg cctcttctct cagacgcgcg agaacatcac
     3841 caagaccgag atcgtgctgc tcatcacgcc gcgcgtggtg cgcacgctgg cgcggccgga
     3901 agtgcgcgcg atcgagttcg ccgccggcac cgaggcttcg ggcggcgcca gcgccttccg
     3961 cggctccgcg ccgccgccgc cgcaagttcc gcagatccag cccgcgccgc agccggagaa
     4021 gccgcccgcg ccgccgccgg ggcagccgca aggcaacacc atggtgccgt tcggcggcgt
     4081 gcagccgccg agatagccat gcggcgcgcg cgcggcttca cgctgatcga gctcttgatc
     4141 acggtggcga tcgtcgcgat cctcgcctcg gccgcgcgcc cgctctcgga gctcaacgtg
     4201 aagcgcgcga aggagctcga gctgcggcgc gcgctgcgcg agatccgcga cgggctcgac
     4261 gcctaccgcc aggcggccga ggacgggcgc atcgagcgcc gcgtcggcga ctccggctac
     4321 ccgcgccgcc tcgaggacct gttcgagggc gtggacgacc gccgcgcacc caacaagcag
     4381 cgcatctact tcctgcgccg cctgccgcgc gacccgatga cgcccgacgg ccagtggggc
     4441 ctgcgcagct acgccagccc gcccgactcg cccgcgcccg gcgacgacgt gttcgacgtc
     4501 tacacgctct ccagcggcgt cggcatcaac ggccgaccgt accgcgagtg gtagagggaa
     4561 gatgcggcta cgcggcttca cgctgatcga gctgctggtg gtgatggcga tcgtcgccac
     4621 gctgctcacg ctcgcggtgc cgcgctactt cgtcagcatc gaccgctcgc gcgaggcggt
     4681 gctgaaggaa aacctgttcc agatccgcga cgcgatcggc aagtaccacg ccgacaaggg
     4741 caggtatccc gaatcgctgc aggtcctcgc caccgagcag tacctgcgca aggtgccgat
     4801 cgatccggtg acgcagagcg agatgacctg ggtggtggtg ccggtgcccg gcaccgaaac
     4861 cgcgggcgta ttcgacgtgc gcagcggcgc gcagggcaag agcaccgacg gaagcgactt
     4921 cgccacgtgg taaatcgcca ggccggcttc acctacatcg gcgtgctgct cgccaccgcg
     4981 atgctcaccg cgggcctggg cctcgccatc gaggcgtggt acaccaccct gcgccgcg
//
LOCUS       DASRIE010000107        12849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1427751, whole genome shotgun sequence.
ACCESSION   DASRIE010000107 DASRIE010000000
VERSION     DASRIE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427751"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFB53_04765"
     CDS             <1..277
                     /locus_tag="VFB53_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461646.1"
                     /note="catalyzes the conversion of ferulic acid to
                     feruloyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HZN86083.1"
                     /translation="DRNEVCALVIPRPGAQDPDLQKLLDALAARSTGSSNRIARALLL
                     PGPLSIDAGEITDKGSVNQRAVLRARADLVELLYAEPAAPPVLKART"
     gene            274..1131
                     /locus_tag="VFB53_04770"
     CDS             274..1131
                     /locus_tag="VFB53_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN86084.1"
                     /translation="MNLEKLVAIDMHTHAWKSALRVGDRPTESQNAMGSYFRYAPQHQ
                     TVPEMADMYRKLGMAFVVFTVDGEKGASRGLSNEEVAELAHKNADVAIPFASINPNRG
                     AEGVKMAQRLIRDYQVRGFKFHPSVQEFYPNDRLAYPLYEAIAEAKLPALFHTGQTGV
                     GAQTPGGGGIRLKYSHPMYLDDVAADFPDMPIILAHPSFPWQEEALSVATHKPQVYID
                     LSGWSPKYFPPILVQYANTLLKEKILFGSDYPVIDPQRWIAEFDKLPIKPEVRPLIMK
                     GNAARLLGL"
     gene            complement(1128..1844)
                     /locus_tag="VFB53_04775"
     CDS             complement(1128..1844)
                     /locus_tag="VFB53_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86085.1"
                     /translation="MRYCKLALLCGVLAASPAWAQNIDQLQAINQGEFRLLSEDLGAA
                     LSFHAQIPTEALGITGFDLGAGLTATKMQNAALLQRVTSDDADATLYVPTLRLHKGLP
                     AGFDIGLTYASVPGSNIRYTGGELRYAILQGGAASPAIGVRGSLTKLSGVDQLAFSTR
                     GIDVSISKGFAFLTPYAGLGRVWVESDPRGTAGLQTEKFSLRKAFVGVGMNFALVNLN
                     LEADKTGDATSTSLKLGWRF"
     gene            1902..3086
                     /locus_tag="VFB53_04780"
     CDS             1902..3086
                     /locus_tag="VFB53_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HZN86086.1"
                     /translation="MTYRTQVGIIGAGPAGLLLAHLLRLEGIESVVLETRAREEIEAT
                     IRAGVLEQGTVDVLNQTGVGARMMREGSVHHGIILRFAGRSHRIDLSGLSGGRSIMVY
                     AQHEVIKDLVQARLDAGAPLEFSVQDVTLQDLESAAPKIRYRDAGGGLRELACDYVAG
                     CDGFHGVSRPAIPSRRRTEFTRVYPFGWFGILVEAPPASEELIYAHHEQGFALVSTRS
                     PAIQRLYLQCDPEDEVKNWPDARIWRELHARLAGADGWRLTEGRIFQKGIVAMRSFVV
                     EPMQYGRLFLAGDAAHIVPPTGAKGLNLAVADVRVLARALAARYRQGRSDMLEQYSAI
                     ALRRVWRAEHFSWWMTSMLHRFHDDTPFHHRLQLAELDYGTSSRAKSTTLAENYVGLP
                     LD"
     gene            3096..4292
                     /locus_tag="VFB53_04785"
     CDS             3096..4292
                     /locus_tag="VFB53_04785"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN86087.1"
                     /translation="MPVRQEILLGRVPVKVYTDDVEERARRQLVNISRLPIVHHHVAA
                     MPDVHHGIGATVGSVIPTLRAIIPAAVGVDIGCGMIAARLSLTANELDEASLKKVFNQ
                     ISRDVPVGFEQHEEREARSDAARPFKRDLLGILQRHPGIEKRVGKKSSWVRQMGTLGG
                     GNHFIEVCLDEAQRVWVMLHSGSRGMGNAIGTYFIALARQDAERHQLQLPDRDLAYFP
                     EGAQHFEDYVAAVAWAQDYARANRAEMMDLVLDALRRHLPAFAVTSEAVNCHHNYVER
                     ETHYGESVWLTRKGAIRARAGELGIIPGSMGTRSYIVRGKGSAEAFQSCAHGAGRRMS
                     RTQAEKQFSVADLAAQTAGVVCRKDRGVLDEIPGAYKDLDAVMANQSDLVEVVHTLKQ
                     VLCVKG"
     gene            4298..4861
                     /locus_tag="VFB53_04790"
     CDS             4298..4861
                     /locus_tag="VFB53_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HZN86088.1"
                     /translation="MISMTRRKLLVAAAALPMARTAPAQDLPLTPACGPQAPLTPRQT
                     EGPYYTPNAPQRSSFVEPGGKGERLFLAGRVVSPQCRPVAKALLDFWHCDEEGDYDNA
                     GFRYRGHLFTDAEGRFRLETIVPALYPGRARHIHVKVQAPGRRLLTTQLYFPGDARDA
                     LWRPELVVAMAQQPRGKAAAFQFIVDA"
     gene            4907..5245
                     /gene="fdxA"
                     /locus_tag="VFB53_04795"
     CDS             4907..5245
                     /gene="fdxA"
                     /locus_tag="VFB53_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HZN86089.1"
                     /translation="MTYVVTEDCIKCKHMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPPKAILPDSDGKAERWLPMNTEFSAKWPNITRKGTPPPDADAWKGVPDKFAK
                     HFTPEPGKGE"
     gene            5248..6489
                     /locus_tag="VFB53_04800"
     CDS             5248..6489
                     /locus_tag="VFB53_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277762492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HZN86090.1"
                     /translation="MELSPEERAILGGARGEAPRRALEYQVKVGDFWGAKRFVPVTNV
                     HMMGDIEVMGDGGLQWLKEHAALGARCAVGTTTNARCIDFAHCERLGQDAGEVAKERE
                     LIACLRRMDVVTTDTCINYQTVYQPHLGEHVAWGDTGTVIYANSVFGARSNFESGPAA
                     LAAAITGRTPEYGFHLDEHRRGSFAVELRARLDDLADWGAVGKLVGEPHQSYTAVPVF
                     HGFHRTPTADELKHLGTALASYGSMGMFHFVGETPEAPSLEAALGGRAPAERMVITDA
                     DLERAYGGYRLGDGDARLVVFSGPQQSLLEMKQLAALFAGRRVQAGASCIVTTNGAVL
                     AQSRALGYAASLEAAGVTILEGVCFYILQNLTPMRLANRWTNMVSNSAKIVNIIGAHR
                     FNTILRRTRDCVDIACTGVLK"
     gene            6486..6896
                     /locus_tag="VFB53_04805"
     CDS             6486..6896
                     /locus_tag="VFB53_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HZN86091.1"
                     /translation="MNLAVARAYGEVVEGEAVVSEEGFSPRYDLDRWSGIITKPGHNL
                     EGTSLKDKICFFPTAKGGIAAGWAFYDIKWKKIAPKAFIFGVTNPVMVQGAVFAGITI
                     TEGWTPHPREVLATGDWVRVDPAARRVEVLRRRP"
     gene            6893..7384
                     /locus_tag="VFB53_04810"
     CDS             6893..7384
                     /locus_tag="VFB53_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86092.1"
                     /translation="MKAILGSLAALMLTACATFDGHTLKPGASTEAEVKALMGAPALE
                     LKRPNGDSWLYFTRYPNGRATFVASIGPDKVLRGIDQRLVYKNIYAVRKGMRAEEVRE
                     LLGPPREVSRLPRLQQDVWEYPWRHAVRELRMLWVQFSDDGVVHTIVEMHDHENDPEN
                     DRG"
     gene            complement(7381..8844)
                     /locus_tag="VFB53_04815"
     CDS             complement(7381..8844)
                     /locus_tag="VFB53_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN86093.1"
                     /translation="MNLAALLHASARRLPGAPAIALGARTVATYGELSQRVARLAAGM
                     LARGLARGDRVALAMRNCPEYYEVLFACWHAGLAAVPINAKLHPKEFAYILGNAGAKL
                     CFVSPDLEAAIPGGLSVASPEYARLGEAAPAAAAEVAPDEVAWLFYTSGTTGVPKGAM
                     LTHRNLMFSTQAYFSDIDRLGPGDSILHAAPLSHGSGVYGLPHFAAGALNVIPESGGF
                     EPGEIFGLVAAHRGVSFFAAPTMIVRLLASPAAGSADLRNLKTISYGGAPMYVADSLR
                     AIELFGPRLYQLFGQGEAPMTITGLPQSDHAARRKLQSCGYARTGAQVRVVDAEDRDL
                     PPGEVGEILTRSDCVMAGYWDNPQATAQALRGGWLHTGDLGSLDEAGYLTIRDRSKDM
                     IISGGSNIYPREIEEVLLRHPAVAECSVVGRAHPEWGEEVVAFIVRRGDVSPQELDAL
                     CLAHIARFKRPREYRFVEGLPKNNYGKVLKTELRKLA"
     gene            complement(8841..9605)
                     /locus_tag="VFB53_04820"
     CDS             complement(8841..9605)
                     /locus_tag="VFB53_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZN86094.1"
                     /translation="MQLADIGANLTHAAFAPDLPEVLERARAAGVETIVVTGTAPEET
                     RKALALGFWTTAGVHPHNAKDCGAGTIAALRTLARDSKVVAIGECGLDFNRNYSPHPD
                     QEKCFVAQLELAAELGKPLFLHSRDAHPRFAEILRNLRVGKAVAHCFTGGAEELRAYL
                     DLGLYIGITGWICDERRGAHLVPLVKEIPADRLLLETDSPYLTPRDLHPQPRARRNEP
                     AHLPHILRAVARARGEPPEALARTTTRNAKAFFGIA"
     gene            complement(9608..11323)
                     /locus_tag="VFB53_04825"
     CDS             complement(9608..11323)
                     /locus_tag="VFB53_04825"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HZN86095.1"
                     /translation="MEYWNLAILVGALLLVVSIVASDISARMGAPLLVVFLALGMLAG
                     EDGPGGIRFDDIDLSYVVGVTGLAVIIFDGGMRTQRESFRVALWPAVSLATLGVVLTA
                     GLVGAFAAWVLDLHWLQGVLVGAIVASTDAAAVFALLRNSGIRLKERVSATLEIESGG
                     NDPMAIFLTVVLLELLAAGRTGFDLHVLAELVKQFGIGTVLGLAGGRLLVWLINRLSL
                     ATGLYPLLAAAGGLFIYGVAAQAGGSGFLAIFLAGLVLGNSRLQAAQNILRVHDGLAW
                     LSQIVLFLLLGLLLTPSELVDVAAPALAIATFLMLVARPLAVVLSLLPFRLPWREQVL
                     ISWVGLRGAVPVVLALFPLIYEAEHARLFFNVAFFIVLVSLLLQGWTIRPAARWLQLE
                     VPPVTEPLQRVTLDIPGHFEHEILCYEVRPGSPIAGRDFGELELPEDKQVMAVMRDGV
                     PQALRAGLQFGAGDYVYLLARPKSLARLSMLFDPHVEPERLEEHRYFGDFVLNGDAVA
                     GEVAAVYGFEVPAGAAEQTLAQYLDEVFRGRVVVGDRLPLGGAQLVVREMEDGRVTRV
                     GLRVR"
     gene            complement(11350..>12849)
                     /locus_tag="VFB53_04830"
     CDS             complement(11350..>12849)
                     /locus_tag="VFB53_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN86096.1"
                     /translation="GQLCLCTPARVCEDAAMLDTGTTPLYHQVADQLHGLIRAGTLRP
                     GERVPSVRRLSRQQGVSVATVLQAYQRLEDAGVIEARPQSGYYVKRASRPVEEPASSK
                     PPRRALAVEINALADVMLQAGQDPKMVSFGSACPAGEMFPLERIRRVLSSRARRDRNA
                     LGRYGLPPGTEALRRAIARRALEWGCRIDHRNLVITNGCMEALNLCLRAVTSPGDTVA
                     LESPTYYGFLQILQALGLKALEIPTHPRTGISLEALELALQAHDVKAVLVMPNVSNPV
                     GATMTEQAKKCLVEMLAARNVPLIEDHIYSELCYDAAARRAAKCFDRTGNVMLCSSFS
                     KTLAPGLKSGFIEPGRWRERIRTLKFVSSGGNAEIIELALAELLESGGYERSLRQLRR
                     RCEQQVDAARGVIAENFPRGTRVTRPTGGFILWVELPKGGDSIALFEKLLERGISIAP
                     GPMFSATQRYRNCLRVSVGHLWTERTEKALAEVGRLARAQIGEGAIANA"
BASE COUNT         2046 a         4450 c         4526 g         1827 t
ORIGIN      
        1 cgaccgcaac gaggtgtgcg cgctcgtcat tccgcggccc ggcgcgcagg acccggacct
       61 gcagaagctc ctcgacgcgc tcgccgcgcg cagcaccggc tcgagcaacc gcattgcgcg
      121 ggccctgctg ctgccgggcc cgctgtcgat cgacgcgggc gagatcaccg acaaggggtc
      181 ggtgaaccag cgcgccgtgc tccgcgcgcg cgccgatctg gtagaactgc tgtatgccga
      241 acctgccgcg ccgccggtgc tgaaagccag gacatgaacc tcgagaagct ggtcgccatc
      301 gacatgcata cccacgcctg gaaatcggcg ctgcgggtcg gcgaccggcc caccgaatcg
      361 cagaacgcga tgggcagcta cttccgctac gcgccgcagc accagacggt gccggagatg
      421 gcggacatgt accgcaagct cggcatggcc ttcgtggtct tcaccgtgga cggggagaaa
      481 ggcgcgagcc gcggcctcag caacgaggag gtcgccgagc tcgcgcacaa gaatgcggac
      541 gtggcgatcc ccttcgccag catcaacccg aaccggggcg ccgagggcgt gaagatggcg
      601 cagcgcctga tcagggacta ccaggtgcgg ggcttcaagt tccacccctc ggtgcaggag
      661 ttctacccca acgaccggct ggcgtacccg ctgtatgaag ccatcgcgga ggcgaagctg
      721 ccggccctgt tccacaccgg gcagacgggc gtgggcgcgc agacgcccgg gggcggcggc
      781 atccgcctga agtattcgca cccgatgtac ctggacgacg tggcggcgga tttccccgac
      841 atgccgatca tcctcgcgca cccttccttt ccctggcagg aggaggcgct gtcggtggcg
      901 acgcacaagc cgcaggtgta catcgacctc tcgggctggt cgccgaaata cttcccgccg
      961 atcctcgtgc agtacgccaa caccctgctc aaggaaaaga tcctgttcgg ctcggactac
     1021 ccggtgatcg atccgcagcg ctggatcgcc gagttcgaca agctgccgat caagccggag
     1081 gtgcggccgc tgatcatgaa gggcaacgcc gctcgcctgc tcggcctcta gaagcgccag
     1141 ccgagcttga ggctggtcga ggtggcgtcg ccggtcttgt cggcctcgag attgaggttg
     1201 accagggcga agttcatgcc gacgccgacg aaagccttgc gcagggagaa tttctccgtt
     1261 tgcagcccgg ccgtgccgcg cgggtcgctt tccacccaga cgcgtcccag ccccgcgtag
     1321 ggcgtgagga aggcgaagcc cttggagatc gagacgtcga tgccgcgcgt gctgaacgcc
     1381 agctggtcga cgccgctcag cttggtgagg ctgccgcgca cgccgattgc cggcgacgcc
     1441 gcgccgccct ggaggatggc gtagcgcagc tcgccgcccg tatagcggat attgctgccc
     1501 ggcaccgagg cgtaggtcag gccgatgtcg aacccggcgg gcagcccctt gtgcaggcgc
     1561 agggtcggca cgtaaagggt ggcgtcggcg tcgtccgagg tgacgcgctg caggagggcg
     1621 gcattctgca ttttcgtggc ggtcaggccc gcgccgagat cgaagcccgt gatgccgagg
     1681 gcctcggtgg gtatttgcgc gtggaagctc agcgccgcgc ccaggtcttc ggacagcagc
     1741 cggaattccc cctggttgat tgcctgcagc tggtcgatgt tctgcgccca ggccggcgac
     1801 gcagcaagaa ccccgcagag caatgcaagc ttgcaatagc gcatgatcgg ctccttgatg
     1861 actggcgata gaatgggtcg gcgcaggttc cgggacgcga cgtgacttac cgcacgcaag
     1921 tcggcattat cggcgcgggc ccggcgggcc tgctgctcgc gcatctgctg cgcctggaag
     1981 gcatcgagtc cgtggtgctc gagacgcgcg cgcgcgagga gatcgaggcg acgatccgcg
     2041 cgggcgtgct ggagcagggc accgtcgacg tactgaacca gaccggcgtc ggcgcgcgca
     2101 tgatgcgcga ggggtccgtg caccacggca tcatcctgcg cttcgcgggc cgcagccacc
     2161 gcatcgacct cagcgggctc tcgggcggac gctcgatcat ggtgtatgcg cagcatgagg
     2221 tgatcaagga tctggtccag gcaaggttgg acgccggcgc gccgctcgag ttcagtgtgc
     2281 aggacgtgac gctgcaggac ctggagagcg cagcgccgaa aatccgctac cgcgacgcgg
     2341 gcggcgggct gcgcgagctc gcctgcgact acgtcgcggg ctgcgacggc ttccacggcg
     2401 tgtcgcggcc cgccatcccg tcgcggcggc gcacggagtt cacccgcgtc taccccttcg
     2461 gctggttcgg catcctggtc gaggcgccgc ccgcctccga ggagctgatc tacgcgcacc
     2521 acgagcaggg cttcgcgctg gtcagcacgc gctcgcccgc catccagcgc ctgtacctgc
     2581 aatgcgatcc cgaggacgag gtgaagaact ggccggacgc gcgcatctgg cgcgaattgc
     2641 acgcgcgcct cgccggcgcg gacggctggc gcctgaccga gggacgcatc ttccagaagg
     2701 gcatcgtcgc catgcgcagc ttcgtggtgg agccgatgca atacggccgg ctgttcctcg
     2761 ccggcgacgc ggcgcacatc gtgccgccga ccggcgccaa ggggctcaac ctcgcggtgg
     2821 ccgatgtgcg cgtgctggcg cgcgcgcttg cggcgcgcta ccggcaggga cgcagcgaca
     2881 tgctcgagca gtactcggcg atcgcgctgc gccgcgtctg gcgcgccgag catttctcgt
     2941 ggtggatgac ctccatgctg caccggttcc acgacgacac cccgttccac caccgcctgc
     3001 agctggcgga actggactac ggcaccagct cgcgcgccaa atcgaccacg ctcgccgaga
     3061 actacgtcgg gctgccgctg gactagactc cggccatgcc ggtgcgccag gaaatcctgc
     3121 tgggccgcgt tcccgtcaag gtctacaccg acgacgtcga ggagcgcgcg cgccgccagc
     3181 tggtcaacat ttcgcggctg ccgatcgtgc accaccacgt cgcggcgatg cccgacgtgc
     3241 accacggcat cggcgccacc gtcggctcgg tgatcccgac gctgcgcgcc atcatcccgg
     3301 cggcggtcgg cgtggacatc ggctgcggca tgatcgccgc gcggctgtcg ctgacggcga
     3361 acgagctcga cgaggcgtcc ctgaagaagg tgttcaacca gatttcccgc gacgtgccgg
     3421 tgggcttcga gcagcacgag gagcgcgagg cgcgcagcga cgccgccagg ccgttcaaga
     3481 gggatctgct cggcatcctg cagcggcacc ccggcatcga gaagcgcgtc gggaagaaat
     3541 cgagctgggt gcggcagatg ggcaccctgg gcggcggcaa ccacttcatc gaggtctgcc
     3601 tcgacgaggc gcagcgcgtc tgggtgatgc tgcattcggg cagccgcggc atgggcaacg
     3661 ccatcggcac ctacttcatc gccctggcca ggcaggacgc cgagcgccac cagctgcagc
     3721 tgcccgaccg cgacctggcg tactttcccg aaggggcgca gcacttcgag gactacgtcg
     3781 cggcggtggc ttgggcgcag gactatgccc gcgccaaccg cgcggagatg atggacctgg
     3841 tgctggacgc gctgcgccgc cacctgccgg cgttcgcggt cacctcggaa gcggtgaact
     3901 gccaccacaa ctatgtcgag cgcgagacgc actacggcga gagcgtgtgg ctgacgcgca
     3961 agggcgccat tcgcgcgcgc gcgggcgagc tcggcatcat cccggggagc atgggcaccc
     4021 gcagctacat cgtgcgcggc aaggggtcgg cggaggcctt ccagtcctgc gcgcacggcg
     4081 cgggccggcg catgagccgc acgcaggcgg aaaaacagtt ctcggtcgcc gacctggcgg
     4141 cgcagaccgc aggcgtggtc tgccgcaagg accggggcgt gctggacgag atccccggcg
     4201 cctacaagga cctcgacgcg gtgatggcga accaatccga cctggtggag gtcgtacaca
     4261 cgctcaagca agtcctctgc gtgaaaggat gaccccgatg atctcgatga cccgccgcaa
     4321 gctgctggtg gccgccgcgg ccctgccgat ggccaggacc gcgccggcgc aggatctgcc
     4381 gctgaccccc gcctgcggcc cgcaggcgcc gctcaccccg cgccagacgg aagggcccta
     4441 ctacacgccg aacgccccgc agcgcagctc cttcgtcgag ccgggcggca agggagagcg
     4501 cctgttcctc gccggccgcg tggtctctcc ccagtgccgg ccggtggcga aggcgctgct
     4561 cgacttctgg cattgcgacg aggaaggcga ctacgacaac gcgggcttcc gctaccgggg
     4621 gcacctgttc acggacgccg aggggcgttt ccgcctggag accatcgttc ctgcacttta
     4681 tcccggcagg gcgcgccaca tccacgtgaa ggtccaggcg ccggggcgcc gcctcctcac
     4741 cacccagctg tattttcccg gcgatgcgcg cgacgcgctg tggcgcccgg agctggtggt
     4801 ggcgatggcg cagcagccgc gcggcaaggc ggccgccttc cagttcatcg tcgacgcctg
     4861 aaaatgacat aatcgcgcct tccgcgattc gcagggcgcg caacagatga cctacgtcgt
     4921 caccgaagac tgcatcaagt gcaagcacat ggactgcgtc gaagtctgtc ccgtcgactg
     4981 cttctatgaa ggcgagaaca tgctcgtcat ccatccggac gagtgcatcg actgcggggt
     5041 gtgcgagccc gagtgcccgc ccaaggcgat cctgccggat tccgacggca aggcggaacg
     5101 ctggctgccg atgaacaccg agttctcggc gaagtggccc aacatcaccc gcaagggcac
     5161 gccgccgccc gacgccgacg cctggaaggg cgtgccggac aagttcgcca agcacttcac
     5221 ccccgagccc ggcaagggcg aataggcatg gagctgtccc ccgaggagcg cgcgatcctc
     5281 ggcggtgcac gcggcgaagc gccgcggcgc gccctggagt accaggtcaa ggtgggcgat
     5341 ttctggggcg cgaagcgctt cgtcccggtc accaacgtcc acatgatggg cgacatcgag
     5401 gtgatgggcg acggcggcct gcaatggctc aaggagcacg cggcgctcgg cgcccgctgc
     5461 gccgtgggca ccaccaccaa cgcgcgctgc atcgatttcg cgcactgcga gcgcctcggg
     5521 caggacgccg gcgaggtcgc caaggaacgc gagctcatcg cctgcctgcg ccgcatggac
     5581 gtggtcacca ccgacacctg catcaactac cagacggtct accagccgca cctgggcgag
     5641 cacgtcgcct ggggcgacac cggcacggtg atctacgcca attcggtgtt cggcgcgcgc
     5701 tccaatttcg agtccggccc ggcggcgctg gccgccgcga tcaccgggcg cacgcccgag
     5761 tacggctttc acctcgacga gcatcggcgc ggcagcttcg cggtggagct gcgcgcgcgc
     5821 ctggacgacc tcgccgactg gggcgcggtg ggcaagctgg tgggcgagcc ccaccagagc
     5881 tacaccgcgg tgccggtgtt ccacggcttc caccgcacgc caaccgcgga cgagttgaag
     5941 cacctgggga cggcgctggc gagctacggc tcgatgggga tgttccattt cgtgggcgag
     6001 acgcccgagg ctccctcgct cgaggcagcg cttggcggcc gcgcgccggc ggagcgcatg
     6061 gtgatcaccg acgccgacct cgagcgcgcc tatggcggct accggctggg cgacggcgat
     6121 gcgcgcctgg tggtgttctc cggcccgcag cagtcgctgc tggagatgaa gcagctggcg
     6181 gcgctgttcg cgggccgccg ggtgcaggcg ggcgcgagct gcatcgtcac caccaacggc
     6241 gcggtcctcg cgcagtcgcg cgcgctgggc tatgcggcga gcctggaggc tgcgggggtc
     6301 acgatcctcg agggggtgtg cttctacatc ctgcagaacc tgacgccgat gcgcctggca
     6361 aaccgctgga ccaacatggt gtccaattcg gcgaagatcg tgaacatcat cggcgcgcac
     6421 cgcttcaaca ccatcctgcg gcgtaccagg gactgcgtgg acatcgcctg caccggggtc
     6481 ctgaagtgaa tctcgccgtc gcgcgggcct acggcgaggt ggtcgaaggc gaggcggtgg
     6541 tgtcggagga gggcttctcc ccgcgctacg acctcgaccg ctggagcggc atcatcacca
     6601 agccggggca caatctcgaa ggcaccagcc tgaaggacaa gatctgcttt ttccccaccg
     6661 ccaaaggggg gatcgccgcc ggctgggctt tctacgacat caagtggaag aaaatcgccc
     6721 ccaaagcgtt catcttcgga gtgacgaatc cggtgatggt gcagggcgcg gtgttcgcgg
     6781 gcatcaccat caccgaaggc tggacgccgc acccgcgcga ggtcctggcc accggcgact
     6841 gggtgcgcgt cgacccggcc gcgcgacggg tcgaggtgct gaggagaaga ccatgaaagc
     6901 aatactgggc agtctggcgg cgctgatgct gacggcttgc gccacgttcg acgggcacac
     6961 cttgaagccc ggggcctcga ccgaggccga ggtgaaggcg ttgatgggcg ctcccgcgct
     7021 cgagctgaag cgcccgaacg gcgattcctg gctgtatttc acgcgctatc cgaacggccg
     7081 cgccaccttc gtcgcgtcca tcggcccgga caaggtgctg cgcggcatcg accagcgcct
     7141 ggtgtacaag aacatctacg ctgtgcggaa gggcatgcgc gcggaggagg tgcgcgagct
     7201 gctcggcccg ccgcgcgagg tgtcgcgcct gccgcgcctg cagcaggacg tgtgggaata
     7261 cccctggcgc cacgccgtgc gcgagctgcg catgctgtgg gtgcagttct ccgacgacgg
     7321 cgtggtgcac accatcgtcg agatgcacga ccacgagaac gacccggaaa acgaccgcgg
     7381 ctaggcgagc ttgcgcagct cggttttcag caccttgccg tagttgttct tcggcaggcc
     7441 ttccacgaag cggtactcgc gcggacgctt gaagcgcgcg atgtgcgcga ggcacagcgc
     7501 gtcgagttcc tgcggcgaca cgtcgccgcg ccgcacgatg aaggccacca cttcctcccc
     7561 ccactccggg tgcgcgcgcc ccaccaccga gcattcggcc accgccgggt ggcgcagcag
     7621 cacttcctcg atctcgcgcg ggtagatgtt cgagccgccc gagatgatca tgtccttgga
     7681 gcggtcgcgg atcgtcaggt agccggcttc gtccaggctg ccgaggtcgc cggtgtgcag
     7741 ccagccgccg cgcagcgcct gcgccgtggc ctgcgggttg tcccagtagc ccgccatcac
     7801 gcagtcggag cgcgtcagga tctcgccgac ctcgcccggc ggcaggtcgc gatcttccgc
     7861 atccaccacg cgcacctgcg ccccggtgcg cgcatagccg caggactgca gcttgcggcg
     7921 cgcggcatgg tccgattgcg gcaggccggt gatggtcatc ggcgcctcgc cctggccgaa
     7981 cagctggtac aggcgcgggc cgaacagctc gatggcgcgc aggctgtcgg cgacgtacat
     8041 cggcgcgccg ccgtaactga tggtcttcag gttgcgcagg tcggcgctgc cggccgccgg
     8101 gctcgccagc agccgcacga tcatggtggg tgcggcgaag aaggacaccc cgcggtgcgc
     8161 cgcgacgagg ccgaagatct ccccgggctc gaagccgccg ctctccggga tcacgttcag
     8221 cgccccggcg gcgaaatgcg gcagcccgta gaccccggag ccgtgcgaca gcggcgcggc
     8281 gtgcaggatc gagtccccgg gcccgaggcg gtcgatgtcg gaaaagtagg cctgggtgga
     8341 gaacatgagg ttgcggtgcg tgagcatcgc gcccttgggc acgccggtgg tgccgctggt
     8401 atagaacagc caggccacct cgtcgggcgc gacctcggcc gctgcggccg gggccgcctc
     8461 gccgagccga gcgtattccg gcgaggcgac cgacagtccg cccggtattg cggcctccag
     8521 gtccgggctg acgaagcaca gcttcgcgcc tgcgttgccg aggatgtatg cgaattcctt
     8581 cggatgcagc ttggcgttga tcggcacagc ggcgaggccg gcatgccagc aggcgaacag
     8641 cacctcgtag tattcggggc agttcctcat cgccagcgcg acgcggtcgc cgcgcgcgag
     8701 gccccgggcg agcatccccg ccgccaggcg cgcgacgcgc tgcgacagct cgccgtaggt
     8761 cgccacggtc cgcgcgccga gcgcgatggc cggcgccccg ggcaggcggc gcgcgctggc
     8821 gtgcagcagt gcggcgaggt tcatgcgatg ccgaagaacg ccttcgcgtt gcgggtcgtc
     8881 gtccgggcaa gggcctcggg cggctcgccg cgcgcgcgcg ccacggcgcg caggatgtgc
     8941 ggcaggtgcg ccggctcgtt gcgccgcgcc ctgggctgcg ggtgcaggtc gcgcggggtg
     9001 aggtagggtg aatccgtctc cagcagcagc cggtccgccg ggatctcctt caccagcggc
     9061 acgaggtggg cgccgcgacg ctcgtcgcag atccagccgg tgatgccgat gtacaggccg
     9121 aggtcgaggt aggcgcgcag ttcctccgcg ccccccgtga agcagtgcgc caccgccttg
     9181 ccgacgcgca ggttcctgag gatctcggcg aagcgcgggt gcgcgtcgcg cgaatgcagg
     9241 aacagcggct tgccgagctc ggcggcgagc tcgagctgcg ctacgaagca cttctcctgg
     9301 tcggggtgcg gcgagtaatt gcggttgaag tccaggccgc attcgccgat ggccaccacc
     9361 ttggagtccc gggccagcgt gcgcagcgct gcgatggtgc ccgcgccgca gtccttggcg
     9421 ttgtgcgggt gcactcccgc ggtggtccag aagccgagcg ccagcgcctt gcgcgtttcc
     9481 tccggcgcgg tgccggtcac gacgatggtt tctacgcccg cggcgcgcgc ccgttccagc
     9541 acctcgggca ggtccggcgc gaacgcggcg tgcgtgaggt tggcgccgat gtccgcgagc
     9601 tgcatgctca gcgcacgcgc aggccgacgc gggtgaccct gccgtcctcc atctcgcgca
     9661 ccaccagttg cgcgccgccc aggggcaggc ggtcgccgac caccacccgg ccgcgaaata
     9721 cctcgtcgag atactgcgcc agcgtctgct cggcggcgcc cgccggcacc tcgaagccgt
     9781 acaccgccgc cacctcgccg gccactgcgt cgccgttgag cacgaaatcg ccgaagtagc
     9841 ggtgctcttc gaggcgctcg ggctcgacgt ggggatcgaa cagcatgctc aggcgcgcca
     9901 ggctcttggg ccgcgccagc aggtagacgt agtccccggc gccgaactgc agtccggcgc
     9961 gcagggcctg cggtacgccg tcgcgcatca ccgccatcac ctgcttgtcc tcgggcagtt
    10021 cgagttcgcc gaaatcgcgg ccggcgatcg ggctccccgg ccggacctcg tagcacagga
    10081 tctcgtgctc gaaatgcccg gggatgtcga gcgtgacgcg ctgcagcggc tcggtgaccg
    10141 gcggcacctc cagctgcagc cagcgcgccg ccgggcggat ggtccagccc tgcagcagca
    10201 gggagaccag tacgatgaag aaggcgacat tgaagaacag gcgcgcgtgc tcggcctcgt
    10261 agatcagggg gaacagcgcc agcaccaccg gcaccgcccc gcgcagcccg acccagctga
    10321 tgagcacctg ctcgcgccag ggcagccgga acggcagcag gctgagcacc accgccagcg
    10381 ggcgcgccac cagcatcagg aaagtggcga tggcgagcgc gggcgcggca acgtccacca
    10441 gctcgctcgg cgtgagcagc agccccagca gcaggaacag gacgatctgg gagagccagg
    10501 cgagcccgtc gtgcacgcgc aggatgttct gcgccgcctg caggcgggaa ttgccgagca
    10561 ccaggcccgc caggaagatg gcgagaaaac cgctgccgcc cgcctgcgca gcgaccccgt
    10621 agatgaacag cccgcccgcc gccgcgagca atggatacag cccggtcgcc agggacaggc
    10681 ggttgatcag ccacaccagc agccggccgc ctgcaaggcc cagcaccgtg ccgatgccga
    10741 actgcttgac gagttcggcc agcacatgga gatcgaagcc ggtgcgcccg gcggcgagca
    10801 gctcgagcag caccacggtg aggaagatcg ccatcgggtc gttgccgccc gattcgatct
    10861 cgagggtggc gctgacgcgc tctttcagcc ggatgcccga gttgcgcagc agcgcgaaga
    10921 ccgccgcggc gtcggtcgag gcgacgatgg cgccgaccag caccccctgc agccagtgca
    10981 aatcgagcac ccaggcggca aaggcgccca ccaggccggc agtgagcacc acccccaggg
    11041 tggcgaggct gacggcgggc cacagcgcga cgcggaagga ttcgcgctgc gtgcgcatgc
    11101 cgccgtcgaa gatgatcacc gccaggccgg tgacaccgac cacgtaggac aggtcgatgt
    11161 cgtcgaacct gatgccgccg gggccgtcct ccccggcgag catgcccagg gccaggaaca
    11221 ccaccagcag cggggcgccc atgcgcgccg agatgtcgct cgccacgatg ctgacgacca
    11281 gcagcagggc gccgaccagg atggcgagat tccagtactc catcccccga acttaccaga
    11341 ggggccgcgt caggcgttcg cgatcgcgcc ttccccgatc tgcgcgcgcg ccagccggcc
    11401 cacctcggcg agcgccttct ccgtgcgctc ggtccacagg tgcccgacgc tcacgcgcag
    11461 gcagttgcgg tagcgctgcg ttgcggaaaa catcggcccg ggcgcgatgg aaatgccgcg
    11521 ctccagcagt ttctcgaaca gcgcgatcga gtcgccgccc ttgggcagct cgacccacag
    11581 gatgaagccg ccggtggggc gcgtgaccct ggtgccgcgc gggaaattct cggcgatcac
    11641 gccgcgcgcc gcgtccacct gctgctcgca gcgccgccgc agctggcgca gcgagcgttc
    11701 gtagccgccg gattcgagca gctcggcgag cgcgagctcg atgatctcgg cgttgcctcc
    11761 gctggagacg aacttcagcg tgcggatgcg ctcgcgccag cgccccggct cgatgaagcc
    11821 cgacttcagc cccggcgcca gcgtcttcga gaacgaactg cacagcatga cgttgcccgt
    11881 gcggtcgaag cacttggcgg cacggcgcgc cgccgcgtcg tagcagagct cggaatagat
    11941 gtggtcctcg atcagcggca cgttgcgcgc ggcgagcatc tccaccaggc acttcttcgc
    12001 ctgctcggtc atggtggcgc ccaccgggtt ggagacgttc ggcatcacca gcacggcctt
    12061 cacgtcgtgc gcctgcaggg cgagctccag cgcctccagg gagatgccgg tgcgcgggtg
    12121 ggtggggatc tccagcgcct tcagcccgag cgcctgcagg atctgcagga agccgtagta
    12181 ggtgggggac tccagggcca cggtgtcccc gggggaggtg accgcgcgca ggcacaggtt
    12241 gagcgcctcc atgcagccgt tggtgatcac caggttgcgg tggtcgatgc ggcagcccca
    12301 ttccagcgcg cggcgcgcga tggcgcggcg cagcgcctcg gtgcccggcg gcagcccgta
    12361 gcggccgagc gcattgcggt cgcggcgcgc gcgcgaggag agcacgcgcc ggatgcgctc
    12421 cagcgggaac atctcgcccg ccgggcaggc cgagccgaag gacaccatct tcgggtcctg
    12481 gcccgcctgc agcatcacgt cggcgagcgc gttgatctcg actgccaggg cgcgccgcgg
    12541 cggcttggag gaggcgggtt cctccaccgg gcgcgaggcg cgcttcacgt agtagcccga
    12601 ctggggccgc gcctcgatca ccccggcatc ctccagccgc tggtaggcct gcagcaccgt
    12661 ggcgacgctc acgccctgct ggcgcgacag tcgcctgacc gagggcaccc gctcgcccgg
    12721 gcgcagggtg ccggcgcgga tcagcccgtg cagctggtcg gcgacctggt ggtagagcgg
    12781 tgtggtgccg gtgtcgagca tcgcagcatc ctcgcaaacg cgggccggcg tgcacaggca
    12841 cagttgccc
//
LOCUS       DASRIE010000108         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_896012, whole genome shotgun sequence.
ACCESSION   DASRIE010000108 DASRIE010000000
VERSION     DASRIE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896012"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFB53_04835"
     CDS             <1..231
                     /locus_tag="VFB53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZN86097.1"
                     /translation="LFPEDAQVEEVQCSTMGVFAPLTGVIGAMQAMEALKLAAEIGES
                     LDGRMVIFDAKSAEWRTVRVKRDPACKVCGKP"
     gene            complement(239..1948)
                     /locus_tag="VFB53_04840"
     CDS             complement(239..1948)
                     /locus_tag="VFB53_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN86098.1"
                     /translation="MLYLLAAYAITLPSGLIEGQARPSGDLVAALKLVCLGAFTWHTV
                     AVLGKRRSAAASALALAATTGLVWTALAGMWLYKVVALTPFDFLAVMVALDDARRTLV
                     AVLGEHGVALALGALALLVLAAGGGAWLLLRLVRAASARLAPGPRVAALCFGLLSYFV
                     AADLWYAANELVLYTRWAAGRPFSVPEPLVPDYSAIAIRSKESVLIVQLESVNARLVF
                     EPTGDAAGYRARIPQPGVETLLKEGGGVFFPLFWANGAGTNRAWESVLCAVSGNVGPP
                     FADDPVRLARRTCLPAKLAETGYSTVLLYSYFDLEFFNLAAFAKRAGFQDVVHGSRLM
                     AEGDRRFSWAYDDCVFYRRAFEYLARQRLDQREQLLAYFEVGMNHAPFLGTMKHPEAH
                     PYREPRNLGEHYLNGVAEQDHCLLEFWRRFQALGRDDIHLFVLPDHGTSVWGLPRVPD
                     PYFATWLAYVPPVRRQAEFRPRVVHAPLPSQAQLYPTILELLGGQAAPASFAFALRGE
                     PAPARYQDCHALGAHGQRLVVYRNGQRSEFDARKGEVVTDGGQRVAASSGTFHQRFGC
                     EVP"
     gene            complement(1984..>2199)
                     /locus_tag="VFB53_04845"
     CDS             complement(1984..>2199)
                     /locus_tag="VFB53_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HZN86099.1"
                     /translation="LQARINQFFDRFEATLKQSLRVASDGDAAGRAGALLRYVIGCLH
                     QYAKSGFAKKPTESFSGQRRYLLTWSA"
BASE COUNT          378 a          735 c          797 g          289 t
ORIGIN      
        1 ctgtttcccg aggacgcgca ggtcgaggag gtgcagtgct cgaccatggg cgtgttcgcg
       61 ccgctgaccg gcgtgatcgg cgccatgcag gcgatggaag cactgaagct cgccgcggag
      121 atcggcgaat cgctcgatgg ccgaatggtg atcttcgatg ccaagagcgc cgagtggcgt
      181 acggtgcggg tgaagaggga tccggcctgc aaggtctgcg gcaaaccctg aattggcgtc
      241 agggcacctc gcacccgaac cgctggtgga aagtgcccga actcgcggcc acccgctggc
      301 ccccgtcggt gaccacctcc cccttgcggg cgtcgaactc gctgcgctgg ccgttgcggt
      361 acacgaccag gcgctgaccg tgtgcgccaa gggcatggca gtcctggtag cgcgccgggg
      421 ccggctcgcc gcgcagggca aaggcaaacg aggccggggc cgcttggccg ccgagcagct
      481 cgaggatggt gggatagagc tgcgcctggc tgggcagcgg cgcgtgcacg acgcgcggcc
      541 tgaattcggc ttggcgacgc accggcggaa cgtaggcgag ccaggtggcg aagtacgggt
      601 ccggcacccg cggcaagccc cacaccgacg tgccgtggtc gggcagcacg aacaggtgga
      661 tgtcgtcgcg gccgagcgcc tggaagcgcc gccagaattc gagcaggcag tggtcctgct
      721 ccgcgacccc gttgaggtag tgctcgccca ggttccgcgg ctcacggtag ggatgcgcct
      781 ccggatgctt catggtgcct aagaagggcg cgtggttcat tccgacctcg aaatacgcaa
      841 gcagctgctc gcgctggtcg aggcgctgcc gggcgaggta ctcgaaggcg cggcggtaga
      901 acacgcagtc gtcgtaggcc caggagaagc gccggtcgcc ttcggccatc aggcgggagc
      961 cgtggaccac atcctggaat ccggcgcgct tcgcgaaggc ggcgaggttg aagaactcga
     1021 gatcgaaata cgagtatagg agcacggtgg aatagccggt ctcggcgagc ttggcgggca
     1081 ggcaggtgcg ccgtgccagg cgcaccgggt cgtcggcgaa cggcgggccg acgttgccgc
     1141 tgaccgcgca cagcacgctt tcccaggcgc ggttggtgcc cgcgccattc gcccagaaca
     1201 gcggaaagaa caccccgccg ccctccttca ggagcgtctc aactcccggc tgcggaatgc
     1261 gcgcgcgata gccggccgcg tcgccggtag gctcgaacac caggcgcgcg ttcaccgact
     1321 cgagctgcac gatcaacacg ctctccttcg agcggatggc gatcgcggag tagtccggca
     1381 ccagcggctc gggcacgctg aacggcctgc ccgccgccca gcgcgtgtac agcacgagct
     1441 cgttcgcggc gtaccagagg tcggcggcga cgaaatagga aagcaggccg aagcagagcg
     1501 cggccacgcg cgggccaggc gccaagcgcg ccgacgcggc ccgcacgagg cgcagcagca
     1561 gccacgcgcc gccgcccgcg gccagcacca gcagcgccag ggcgccgagc gcaagggcca
     1621 cgccatgctc gcccagcacc gcgaccaggg tgcgccgcgc gtcgtcgagc gcgaccatca
     1681 ccgcgaggaa gtcgaaaggc gtcagcgcca cgaccttgta cagccacatg ccggcgagcg
     1741 ccgtccacac caggccggtc gtcgcggcga gcgcgagcgc gctcgccgcc gcgctgcgcc
     1801 gcttgccgag caccgccacc gtgtgccagg tgaaggcccc gaggcagacg agcttgagcg
     1861 cggcgaccag gtcgcccgag ggccgcgcct ggccctcgat cagtccgctc ggcagcgtga
     1921 tcgcgtaggc ggccagcagg tagagcaaag cgccagccat tccgctggct gcgagagcgg
     1981 gcgttaggcg gaccaagtca acagataacg tctttgccct gaaaagcttt cggtgggttt
     2041 cttcgcgaag ccgctcttcg cgtactggtg caggcagcca atgacgtagc gcagcagcgc
     2101 acccgctctg ccggcggcgt cgccgtcgga ggcgacgcgc agggactgct tgagcgtggc
     2161 ttcgaagcgg tcgaagaact ggttgatgcg cgcctgcag
//
LOCUS       DASRIE010000109         5817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1430004, whole genome shotgun sequence.
ACCESSION   DASRIE010000109 DASRIE010000000
VERSION     DASRIE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430004"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFB53_04850"
     CDS             complement(<1..1097)
                     /locus_tag="VFB53_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86100.1"
                     /translation="MRSPTALLLGGLNLVRALGLGRIAHVVASPQPDWPAAWSRFATG
                     SLLLPRLERREAVVRALLDTGKRLADAAGARVPLYYGNDDYLSLVQESYGELRRFFRL
                     LLNPPEVARALIDKERFEAFARGRGLPVPRTLAWDELADWAHPVLVKPKVKIGYDDSA
                     VYQRLFGGFGKARVFSSGAELAASALASQLRDDLLIQEYVRGDDRNLWSFHGYADEDG
                     GLLASFVGRKIRTWPPITGESSFIELAHDDAFALVGRQIAAQATLRGVFKIDFKRDAV
                     SGAWRVLEINARFNLWHYLGACNGINLPRIAYDYLVYGARPAATPYSTTRRWLSLRND
                     FRAFRRLAAHGELDLGGWLRSLVEAPKVYDF"
     gene            1212..1931
                     /locus_tag="VFB53_04855"
     CDS             1212..1931
                     /locus_tag="VFB53_04855"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HZN86101.1"
                     /translation="MIVAIHQPHFLPWLGYLHRMAQVDLFVLLDHVQFERRNYQNRTM
                     IRMNGEPRWLTVPVVQRSQKERILDKEVDNRPDGSKTWGETHFATLRHAYARAPFFAE
                     YAGAFEALFARPWQRLVELNQASLDLLREAFAIRTPLARSSELAVEGARGDLILGICR
                     AVGARTLMAGMGGSRGYLDTQMFAQAGIDIRYHDFAHPEYPQCGDAPFSRGLSAIDLL
                     FNCGAGSREVLFTRPPVAQAA"
     gene            1928..2605
                     /locus_tag="VFB53_04860"
     CDS             1928..2605
                     /locus_tag="VFB53_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZN86102.1"
                     /translation="MNAPLPPKAFQQRSISLASRYGGRTVLAIGAHPDDLELAVGGTL
                     ARLSRESVRVVMAIVSIPSSYETRKAEAEHAARILGCELRLMMDKPCRIDDMKNYQLV
                     GMLDELVREFKPAAVLTHSANEFHRDHVAVHNACFSTQRLQPFDFLHFNPTMTRAVPV
                     SFHPRAYIDVSGTIELKMKAIEAHASQFGERGLDTDMWRDIARVNGRMVGVKYAEGLD
                     VSRMLLA"
     gene            2602..2976
                     /locus_tag="VFB53_04865"
     CDS             2602..2976
                     /locus_tag="VFB53_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86103.1"
                     /translation="MRAGLLIVPVLLGACAAPPPADETRVAIVWNRVDDAHRVCESLS
                     GRKNFFNILGCSQRIEPAEAGAPRVCAIYAPAPRSERDLQRFATLGHELLHCFDGNWH
                     DQWGRMTSPPSAAAGGTFTSIE"
     gene            2973..4322
                     /locus_tag="VFB53_04870"
     CDS             2973..4322
                     /locus_tag="VFB53_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86104.1"
                     /translation="MKFVTERLKRLNLGDALVRRNPFYYRRARARISEMEKLPLEERR
                     QRTEQRLGKVLAVARRTAYGRRAGGGEALSSWPLLEKAALRAEPEAFRAPGLFVSVRG
                     STGGTTGVPLRLVRSLQSVVLEQAALDALLERLGVRPGRERMAVLRADSIKDPAERSP
                     PYWILTHGGRRMVLSPHHLCAETVAQYARALSEFRPDILWVYPTAVESLCRLLLGAGL
                     RLEIPRIYASSEVLGAQAWKLARLALGGPAIADYYGQAERVAFAAALEPGVYRFLPGY
                     SAVELVPFAPDGDGTLYEIVGTSLWNLAMPLVRYRTGDLVRLPAAWGAAELEEVALGI
                     RPFGGVLGRSGEFLLTPEGARITGINHFPREVQHVRRIQVIQERLDQVRLLVLADAGY
                     APHDAQRLLANVRAKLPPSMRVSVELCERLEQTALGKTPFVIHRPAVRKLVELADAA"
     gene            4378..5433
                     /locus_tag="VFB53_04875"
     CDS             4378..5433
                     /locus_tag="VFB53_04875"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HZN86105.1"
                     /translation="MDRPRLVQALAGQRGYCESRSPLYAAILAGLQSDPDAPWLGAAE
                     CAWRGRQFAVDWEAAHLLLAGMHFWALKGEAPELAAVYPSCNGGGNDAAGAARAFLAR
                     APDEFWQYLATARVQTNEAGRSVPWMLAAAAAFGPRALPFSLVELGASAGLNLVGDWL
                     EHRCHFTAGNGVPATPPAGWNRRPHRVLARAGLDLAPRRLADPEDRLWLKACVWADDL
                     ARLGRLEDSIKTFLKRERTVRLERCAFADAPAWLAAHFPPRERQGLLVFNSIGTVYLD
                     DAAYAELQRGMAQALEPWGDRGFWAEYERPRGRDGPLELRIHRPAGGALRTRVLASGA
                     PRPTVMRFHEATPWRLD"
     gene            5418..>5817
                     /locus_tag="VFB53_04880"
     CDS             5418..>5817
                     /locus_tag="VFB53_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86106.1"
                     /translation="MATRLKQTVSARWLCGRMGLELIGPDRDIHQICPLEALAEGGLS
                     FVRPGRKAPAAACGTIVAAKGFPFRGASVIGSPDPRLDFIRAQYLLARFPGFERASTP
                     PEIHPTAQVAPSAVIENGVAIGEGTVVGPNA"
BASE COUNT          814 a         2115 c         2050 g          838 t
ORIGIN      
        1 aagaagtcgt acaccttggg cgcctcgacc agcgagcgca gccatccgcc gaggtcgagc
       61 tcgccgtgtg ccgccagccg gcggaaggcg cggaaatcgt tgcgcagcga gagccagcga
      121 cgcgtggtgc tgtatggcgt ggcggcgggc cgcgcgccgt acacgagata gtcgtacgcg
      181 atgcgtggca ggttgatgcc gttgcaggcg ccgaggtagt gccacaggtt gaagcgcgcg
      241 ttgatctcga gcacgcgcca ggcgccgctg accgcatcgc gcttgaagtc gatcttgaat
      301 acgccgcgca gcgtcgcttg cgcggcgatc tgccggccga ccaacgcgaa ggcgtcgtcg
      361 tgcgccagct cgataaagct cgactcgccg gtgatcggcg gccaagtccg gatcttgcgc
      421 ccgacgaacg aggcgagcag cccgccgtcc tcgtcggcat agccgtggaa cgaccacaag
      481 ttgcggtcgt cgccgcgcac gtactcctgt atcagaagat cgtcgcgcag ctggctcgcg
      541 agcgccgagg cggcgagctc ggcgccgctc gagaacacgc gcgccttgcc gaagccgccg
      601 aagaggcgct ggtacaccgc cgagtcgtcg tagccgatct tcaccttcgg cttcaccagc
      661 accggatgcg cccagtcagc gagctcgtcc caggcgagcg tgcgcggcac cggcaggccg
      721 cggccgcggg cgaacgcctc gaagcgctcc ttgtcgatca gcgcgcgcgc cacttccggc
      781 ggattgagca gcaggcggaa gaagcgccgc agctcgccat agctctcctg gaccaggctc
      841 aggtagtcgt cgttgccgta atacagcggc actctggccc ctgccgcgtc cgccaggcgc
      901 ttgcccgtgt cgagcagcgc ccggaccacc gcctcgcgcc gctcgagccg cggaagcagc
      961 aggctgcccg tcgcgaagcg cgaccatgcc gccggccagt cgggctgcgg cgacgccacc
     1021 acatgcgcga tgcggcccaa gccgagcgca cgcaccaagt tcaggccgcc gagcagcagt
     1081 gcggtcgggc ttctcattgc aaagaggcta acgggcgccg tgccgaaaca gtccggggcg
     1141 gctctgcgcc aagcggctta cgaggcgcag gccatgggtt gcgggcgcgc tcggtacgct
     1201 gagcgcctcc catgatcgtc gcgatccacc agccgcactt cctgccgtgg ctcggctacc
     1261 tgcatcgcat ggcgcaggtg gacctgttcg tgctgctcga ccacgtgcag ttcgagcgcc
     1321 gcaactacca gaaccgcacc atgatccgca tgaacggcga gccgcgctgg ctcaccgtgc
     1381 cggtggtgca gcgctcgcag aaggagcgca tcctcgacaa ggaagtcgac aaccgccccg
     1441 acggctcgaa gacctggggc gagacccatt tcgccacgct gcgccacgcc tacgcccgcg
     1501 cgccgttctt cgccgagtac gccggcgcgt tcgaggcgct gttcgcgcgg ccatggcagc
     1561 ggctggtcga gctgaaccag gcctcgctcg acctgctgcg cgaggccttc gccatccgca
     1621 cgccgctcgc gcgcagctcc gagctcgcgg tcgagggcgc gcgcggcgac ctgatcctcg
     1681 gcatctgccg cgcggtcggc gcgcgcacgc tgatggccgg catgggcggg tcgcgcggct
     1741 acctcgatac gcagatgttc gcgcaggccg ggatcgacat ccgctaccac gatttcgcgc
     1801 atcccgaata tccgcagtgc ggagacgcgc ccttcagccg cgggctctcc gcaatcgacc
     1861 tgctgttcaa ctgcggcgcg ggcagccgcg aggtgctgtt cacccggccg ccggtggcgc
     1921 aggccgcatg aacgcgccgc tgccccccaa ggcattccag cagcgcagca tctcgctcgc
     1981 ctcgcgctac ggcgggcgca cggtgctcgc gatcggggcg caccccgacg acctcgagct
     2041 cgccgtcggc ggcacgctcg cgcgcctgtc gcgcgagtcg gtgcgcgtgg tcatggcgat
     2101 cgtctcgatc ccttcgagct acgagacgcg caaggccgag gccgagcacg ccgcccggat
     2161 cctcggctgc gagctgcggc tgatgatgga caagccctgc cgcatcgacg acatgaagaa
     2221 ctaccagctc gtcggcatgc tggacgagct ggtgcgcgag ttcaagccgg ccgcggtgct
     2281 gacgcacagc gcgaacgagt tccaccgcga ccacgtcgcc gtgcacaacg cctgcttctc
     2341 gacgcagcgg ctgcagccct tcgacttcct gcacttcaac ccgaccatga cgcgcgcggt
     2401 gccggtgtcg ttccatccgc gcgcctacat cgacgtcagc ggcaccatcg agctcaagat
     2461 gaaggcgatc gaggcgcacg cgagccagtt cggcgagcgc ggcctggaca ccgacatgtg
     2521 gcgcgacatc gcccgcgtca acggccgcat ggtcggcgtg aagtacgccg agggcctcga
     2581 cgtcagccgc atgctgctcg cgtgagagcc ggtcttctca tcgtccccgt cctgctcggc
     2641 gcctgcgcgg cgccgcctcc cgcggacgag acgcgcgtcg ccatcgtgtg gaaccgcgtc
     2701 gacgacgcgc accgcgtgtg cgagtcgctc tccgggcgca agaacttctt caacatcctc
     2761 ggctgctcgc agcgcatcga gcccgccgag gccggcgcgc cgcgcgtctg cgcgatctac
     2821 gccccggcgc cgcgcagcga gcgcgacctg cagcgcttcg ccaccctcgg gcacgagctg
     2881 ctgcactgct tcgatggcaa ctggcacgac cagtggggcc gcatgacgag cccgccgagc
     2941 gcggccgccg gcggcacgtt tacgtcgatc gaatgaagtt cgtcaccgag cggttgaagc
     3001 gcctgaacct cggcgacgcg ctcgtgcgcc gcaacccgtt ctactaccgc cgtgcccgcg
     3061 cccggatttc cgagatggag aagttgcctt tggaagaacg ccggcagcgc accgagcagc
     3121 gcctcgggaa agtactggcc gtcgcgaggc gcaccgctta cggccgccgc gccggcggcg
     3181 gcgaggcgct ctcgtcctgg ccgctgctcg agaaggcggc gctgcgcgcc gagcccgagg
     3241 cgttccgcgc cccggggctc ttcgtctcgg tgcgcggcag caccggcggc accaccggcg
     3301 tgccgctgcg cctggtgcgc tcgctgcagt cggtggtgct ggagcaggcg gcgctcgacg
     3361 cgcttctcga gcgcctgggc gtgcggccgg ggcgcgagcg catggcggtg ctgcgcgccg
     3421 acagcatcaa ggaccctgcc gagcgctcgc cgccctactg gatcctgacg cacggggggc
     3481 ggcgcatggt gctctcgccg caccacctgt gcgcggaaac cgtggcccag tacgcgcgcg
     3541 cgctttccga attccggccc gacatcctgt gggtgtatcc gaccgcagtg gagtcgctgt
     3601 gccgcctgct gctcggcgcc ggcctgcggc tcgagatccc gcggatctac gcgtcttccg
     3661 aggtgctggg cgcgcaggcc tggaagctcg cgcgcctcgc gctcggcggc ccggcgatcg
     3721 ccgactacta cggccaggcc gagcgcgtcg ccttcgcggc cgcgctggag cccggcgtgt
     3781 accgcttcct gccgggttac tcggcggtgg agctggtgcc gtttgcgccc gacggcgacg
     3841 gcacgctcta cgagatcgtc ggcacctcgc tctggaacct ggcgatgccg ctcgtgcgct
     3901 accgcaccgg cgacctggtg cggctgcccg ccgcgtgggg cgccgcggag ctcgaggagg
     3961 tggccttggg cattagaccg ttcggcggag tcctcggccg cagcggcgag ttcctgctca
     4021 cgccggaggg cgcgcgcatc accggcatca accacttccc gcgagaggtg cagcacgtgc
     4081 gccgcatcca ggtgatccag gagcggctcg accaggtgcg gctgctggtg ctcgccgatg
     4141 ccggctatgc gccgcacgac gcgcagcgat tgctcgccaa cgtgcgcgcg aagctgccgc
     4201 cttcgatgcg cgtctcggtg gagctgtgcg agcgcctgga gcagaccgcg ctcggcaaga
     4261 cgccgttcgt gatccaccgt cccgcggtgc gtaagctggt cgagctagcc gacgcagcgt
     4321 agcgacagtg ccgcgcgggg gatcgcgcgg cccccggccg cagcctaagg tggacgcatg
     4381 gaccgcccgc gcctcgttca agcgctggcc ggccagcgcg gttactgcga atcccgttca
     4441 ccgctctatg ccgcgatact agccggcctg caatccgatc ccgacgcacc gtggctcggc
     4501 gccgcggaat gcgcctggcg cgggcggcag ttcgcggtgg attgggaagc cgcgcacctg
     4561 ctgctcgccg gcatgcactt ctgggcgctg aagggcgagg cgccggagct cgcggcggtc
     4621 tatccgagct gtaacggcgg gggcaacgat gccgccgggg cggcacgggc gttcctcgcg
     4681 cgcgcgcccg acgaattctg gcagtacctc gccaccgccc gcgtccagac caacgaggct
     4741 gggcgcagcg tcccctggat gctcgcggcg gcggcggctt tcggcccgcg cgcgctgccg
     4801 ttcagcctgg tggagctcgg cgcctccgcc gggctcaacc tggtgggcga ctggctcgag
     4861 caccgctgcc atttcaccgc gggaaacggc gtcccggcga cgccgcccgc cggctggaac
     4921 cgccggccgc atcgcgtgct cgcccgcgcc gggctggacc tcgcgccgcg gcgcctcgcc
     4981 gatcccgagg accggctgtg gctcaaggcg tgcgtctggg ccgacgacct ggcgcgcctc
     5041 ggcaggctgg aggactcgat caagaccttc ctgaagaggg agcgaacggt ccgcctggag
     5101 cgctgcgcct tcgccgacgc cccggcgtgg ctcgccgccc atttcccgcc tcgcgagcgc
     5161 cagggcctgc tggtgttcaa ctcgatcggc acggtctacc tggacgacgc ggcctacgcc
     5221 gagctgcagc gcggcatggc gcaggcgctc gagccctggg gcgaccgcgg cttctgggcc
     5281 gagtacgagc gtccgcgcgg ccgcgacggg ccgctcgagc tcaggatcca ccgacccgcc
     5341 ggcggggcgc tgcgcacccg cgtgctcgct tccggcgcgc cgaggccgac ggtgatgcgc
     5401 tttcacgagg cgacgccatg gcgactcgat tgaagcaaac ggtttcggcg cgctggctgt
     5461 gcgggcggat gggcctcgag ctgatcggcc cggaccggga tatccaccag atctgcccgc
     5521 tcgaggcgct ggccgaaggc gggctgtcgt tcgtgcgtcc gggccggaaa gcgccagccg
     5581 cggcctgcgg caccatcgtg gctgcgaagg ggttcccgtt caggggagcg tcggtcatcg
     5641 gctccccgga cccgcgcctg gatttcatcc gcgcgcaata cctgctggcg cgattccccg
     5701 gcttcgagcg cgcgagcacg ccgccggaga tccatcccac ggcgcaggtg gcgccgagcg
     5761 cggtgatcga gaacggcgtg gcgatcgggg agggcacggt ggtcggcccg aacgcgg
//
LOCUS       DASRIE010000110         6085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_897875, whole genome shotgun sequence.
ACCESSION   DASRIE010000110 DASRIE010000000
VERSION     DASRIE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897875"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFB53_04885"
     CDS             complement(<1..329)
                     /locus_tag="VFB53_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86107.1"
                     /translation="MAESQDVNALKRRGRRRLVGAIALVLAAVIVLPWIFDQNPRQAP
                     PVSVRIPNEDDSPFLSKVAPKPPPPLPAEKALKPEAAAAPAQAAKPEAKPVPPKPAPP
                     PANERASA"
     gene            complement(322..1611)
                     /gene="folC"
                     /locus_tag="VFB53_04890"
     CDS             complement(322..1611)
                     /gene="folC"
                     /locus_tag="VFB53_04890"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZN86108.1"
                     /translation="MSARGLADWLDFIGRQHPDAIALGLERVREVLSRMGTRLACPVI
                     SVAGTNGKGSVCAMLESILRASGRSTGLYTSPHLLRYGERVRIDGGEAPEGALCEAFE
                     AVERARGGVPLTYFEYGTLGALWLFARAAPDVLVLEVGLGGRLDAVNVVDADCAVITS
                     VALDHVELLGATREAIGREKAGIFRRDRPAVVADPQPPSSVLDEAARIGARLSLLERD
                     FGCRAHPGQWDYWGPGGKRRSLAPPALRGARQLRNACAALAALDALDARLPVSMQDVR
                     RGLAEVVLPGRFQVLPGRPQVILDVAHNPEAAQVLADNLGDAGFAPETIGVFGALRDK
                     DIPGVARALAPRITRWHLGGLAGPRGSSAGELARALDAAGVAAPRAEFPSVRQALAAA
                     RGMAGENDKIAVFGSFLTVADAMRELAAGASGRPTYG"
     gene            complement(1608..2477)
                     /gene="accD"
                     /locus_tag="VFB53_04895"
     CDS             complement(1608..2477)
                     /gene="accD"
                     /locus_tag="VFB53_04895"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZN86109.1"
                     /translation="MSWLQKLLPPKIQRSSGPKRAVPEGLWTKCPACDAVLYSTDLEK
                     NLNVCPKCGFHQRIGARARLDALLDADGRFEIGAEVLPVDTLKFKDSKKYTERLLEAE
                     AQSGESEALVVLQGAIKSIGVVAAAHEFEFMAGTMGSVLGERFVRGVRVAIEQKLPFL
                     AITASGGARMQEGLLSLLQMAKTTAALTDLAERGLPFITVLTDPTTGGVSASYAMIGD
                     LVLAEPKALVGFAGPRVIEQTVRQKLPEGFQRAEFLLEKGAVDMIVDRRQMRDKLATL
                     LGLLQGIATDRPS"
     gene            complement(2474..3271)
                     /gene="trpA"
                     /locus_tag="VFB53_04900"
     CDS             complement(2474..3271)
                     /gene="trpA"
                     /locus_tag="VFB53_04900"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZN86110.1"
                     /translation="MSRISGRFAELKAAGRKALIPYVTAGDPLKASTVPLLHCMVEAG
                     CDILEIGVPFSDPMADGPAIQRASERALKNGIGLSDVLGLVRQFREKDSTTPIVLMGY
                     ANPIEAMGVGKFVAAAKAASVDGVIVVDYPPEECGPFADLCGKAGIDCIFLLAPTSTD
                     ARIREVVRAGTGYLYYVSLKGVTGVKTADAADIATHLPRLRAASKLPIAVGFGIKDAE
                     SARVISKGADAVIIGTRIVQEIENAGPEHAVARVKALLIPIRKALDS"
     gene            complement(3271..4464)
                     /gene="trpB"
                     /locus_tag="VFB53_04905"
     CDS             complement(3271..4464)
                     /gene="trpB"
                     /locus_tag="VFB53_04905"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZN86111.1"
                     /translation="MHDLPDARGHFGPYGGVFVAETLSHALEELRDAYERYRRDPEFV
                     AEFRYELKHYVGRPSPIYHARRWSQVLGGAQIYLKREDLNHTGAHKINNTIGQALLAR
                     RMGKKRIIAETGAGMHGVASATVAARLGMPCVVYMGSEDIKRQAQNVYRMKLLGATVV
                     PVESGSKTLKDALNEAMRDWVTNVEDTFYIIGTVAGPHPYPMMVRDFQSVIGEECQRQ
                     MPELAGRQPDAVIACVGGGSNAMGIFYPYIGEEKVRLIGVEAAGEGLHTGKHAASLCA
                     GTPGVLHGNRTYLLQDANGQIVETHSVSAGLDYPGVGPEHAWLKDQHRAEYVAVTDDE
                     ALKAFDDLCRYEGIIPALESAHAVAYAAKIAPALTKDTLLLVNLSGRGDKDMHTVAER
                     RGLKL"
     gene            complement(4457..5077)
                     /locus_tag="VFB53_04910"
     CDS             complement(4457..5077)
                     /locus_tag="VFB53_04910"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZN86112.1"
                     /translation="MRTRVKICGITRPGDAEAAARAGADAIGLVFYPPSPRFLSVERA
                     REIRAALPPFVQAVALFVNPDAAQVAQVIGRLHPAMLQFHGEETPEFCAQFGLPYLKA
                     CRVAPGVDLLKYLQPFSAASGWLLDAHVAEYGGVGAGFDWSLVPARLERPLVLSGGLA
                     SGTVGQAVRRLRPWAVDVSSGVESAKGIKDAAKIAAFIAEVRNADA"
     gene            complement(5074..5865)
                     /gene="truA"
                     /locus_tag="VFB53_04915"
     CDS             complement(5074..5865)
                     /gene="truA"
                     /locus_tag="VFB53_04915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZN86113.1"
                     /translation="MRIALALEYDGSRFLGWQTQPGGGTVQDALQEALGAIAGERIGV
                     TCAGRTDRGVHALGQVVHFDTGAARPGSAWVRGANALLPESVAVQWAQEVPPEFHARY
                     GARERSYRYVLINRAVRPALAARHAGWFHLPLDVAAMRAAARHLVGEHDFSSFRSAEC
                     QAKSPVRTLLALDIETRGERIDFVLRANAFLHHMVRNIVGTLVTVGKGKQPPAWAGAV
                     LDARDRARAAPTFPAQGLYLERVAYAASWGLPQQASPAQRIPVLP"
     gene            complement(5945..>6085)
                     /locus_tag="VFB53_04920"
     CDS             complement(5945..>6085)
                     /locus_tag="VFB53_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZN86114.1"
                     /translation="ATKLDLAKAYEEMGDKDGARELLNEVVREGDGAQQSQANQMLAK
                     LG"
BASE COUNT          859 a         2235 c         2086 g          905 t
ORIGIN      
        1 gcgctcgcac gctcgtttgc gggcggcggc gccggcttcg gcggcacggg cttcgcctcg
       61 ggcttggctg cctgcgccgg cgccgcggcc gcttcgggtt tcagcgcctt ctcggccggc
      121 agcggcggcg gcggcttggg cgcgaccttg gacaggaagg gcgagtcgtc ctcgttcggg
      181 atgcgcacgc tcaccggcgg cgcctggcgc ggattctggt cgaagatcca tgggagcacg
      241 atcacggcgg cgagcaccag cgcgatcgcg ccgaccagcc gccgccgccc gcggcgcttc
      301 agcgcattga cgtcctggga ctcagccata ggtggggcgg ccgcttgctc cggccgctaa
      361 ttcgcgcatc gcatccgcta cggttaggaa cgaaccgaac accgcgattt tatcattctc
      421 gcccgccatc ccgcgggccg cggcgagcgc ttggcgcacc gacgggaatt cggcgcgcgg
      481 cgccgccacg ccggcggcat cgagcgctcg cgcgagctcg ccggcgctgc tgccgcgcgg
      541 ccccgcgaga ccgccgaggt gccaccgggt gatgcggggc gccagcgcgc gcgccacgcc
      601 ggggatgtcc ttgtcgcgca gcgcgccgaa cacgccgatc gtctccggcg cgaagccggc
      661 gtcgccgagg ttgtccgcca gaacctgcgc cgcctccggg ttgtgcgcga catcgaggat
      721 cacctggggc cggccgggaa gcacctggaa gcgcccgggc agcaccactt ccgccaggcc
      781 gcgccgcacg tcctgcatgc tcaccggcag gcgcgcgtcc agggcgtcca gcgcggcgag
      841 cgcggcgcag gcgttgcgca actggcgtgc cccgcgcagc gccgggggcg caagcgaccg
      901 gcgcttgccg cccgggcccc agtagtccca ttgtcccgga tgcgcgcgac agccgaaatc
      961 gcgctcgagg agcgatagcc gcgcgccgat gcgcgccgcc tcgtcgagca ccgaggacgg
     1021 cggttgcggg tcggcgacga ccgccgggcg atcgcgccgg aagatgccgg ccttctcccg
     1081 cccgatcgcc tcgcgcgtcg cgccgaggag ctcgacgtgg tccagcgcga cgctggtgat
     1141 caccgcgcaa tccgcatcca ccacgttgac cgcgtccagg cggccgccca ggccgacttc
     1201 cagcaccagc acatcgggcg ccgctcgcgc gaacagccac aatgcgccga gcgtgccgta
     1261 ttcgaaatag gtcagcggca ccccgccgcg tgcgcgctcc accgcctcga acgcctcgca
     1321 cagcgcgccc tccggcgcct cgccgccgtc gatgcgcacg cgctcgccgt agcgcagcag
     1381 gtggggcgag gtgtacagcc cggtgctccg gccggaggcg cgcaggatgc tctccagcat
     1441 ggcgcagacc gagcccttgc cgttggtccc ggcgacgctg atcaccgggc aggcgaggcg
     1501 ggtgcccatg cgcgagagca cctcgcgcac gcgctccagg ccgagcgcga tggcgtccgg
     1561 gtgctggcgc ccgatgaaat ccagccagtc cgcgagcccg cgcgcgctca cgaaggccgg
     1621 tcggtggcga ttccctgtag caggccgagc agcgtcgcca gcttgtcgcg catctggcgg
     1681 cggtcgacga tcatgtccac cgcgcccttc tccagcagga attcggcgcg ctgaaaaccc
     1741 tccggcagct tctggcgcac ggtctgctcg atcacgcgcg ggccggcgaa gccgaccagc
     1801 gccttcggct cggcgagcac caggtcgccg atcatggcgt agctcgccga caccccgccg
     1861 gtggtggggt cggtcagcac ggtgatgaac ggcaggccgc gctcggcgag atccgtcagc
     1921 gcggcggtgg tcttggccat ctgcagcagc gacagcaggc cctcctgcat gcgcgccccg
     1981 ccgctcgcgg tgatggcgag gaacggcagt ttctgctcga tcgccacgcg cacgccgcgc
     2041 acgaagcgct cgccgagcac cgagcccatg gtgcccgcca tgaactcgaa ctcgtgcgcc
     2101 gccgccacca cgccgatcga cttgatcgcc ccctgcagca ccaccagcgc ctcggattcg
     2161 cccgactgcg cctcggcctc cagcaggcgc tcggtgtatt tcttggagtc cttgaacttc
     2221 agcgtgtcca ccggcagcac ttcggcgccg atctcgaagc ggccgtcggc atcgagcagc
     2281 gcatcgagcc gcgcgcgcgc gccgatacgc tggtggaagc cgcacttcgg gcagacgttg
     2341 aggttcttct ccaggtcggt gctgtagagc accgcgtcgc aggccgggca cttggtccac
     2401 aggccctcgg gcaccgcgcg cttgggcccg gagctgcgct ggatcttcgg cggcaggagc
     2461 ttttgcagcc agctcatgaa tccagcgcct tgcggatggg gatgagcagg gccttgacgc
     2521 gcgccacggc gtgctcgggc cccgcgttct cgatctcctg cacgatgcgg gtgccgatga
     2581 tcacggcgtc ggcgcctttc gagatcacgc gcgccgattc ggcgtccttg atgccgaagc
     2641 cgacggcgat cggcagcttg gaagcggccc gcaaccgcgg cagatgcgtc gcgatatcgg
     2701 cggcatccgc cgttttcacg cccgtcacgc ccttgagcga gacgtagtag aggtagcccg
     2761 tgccagcgcg caccacttcg cggatgcgcg cgtccgtgga ggtgggcgcc agcaggaata
     2821 tgcagtcgat gccggccttg ccgcatagat cggcgaacgg cccgcactcc tccggcggat
     2881 agtccaccac gatgacgccg tccacgctgg ccgccttggc tgcggcgacg aattttccga
     2941 cgcccatggc ctcgatcgga ttggcatagc ccatcaggac gatcggcgtt gtcgagtcct
     3001 tctccctgaa ctgccggacc aggcccagca cgtccgagag cccgatgccg ttcttcagcg
     3061 cgcgctcgga ggcgcgctgg atcgccgggc cgtcggccat cgggtcggag aacggcacgc
     3121 cgatctcgag gatgtcgcag ccggcttcca ccatgcagtg cagcagcggc accgtgctcg
     3181 ccttcagcgg gtcgcccgcg gtgacatagg gaatcagcgc cttgcgcccg gcggccttca
     3241 gttcggcgaa gcgcccggag atgcgcgaca tcagagcttc agcccgcggc gctcggcgac
     3301 ggtgtgcatg tccttgtcgc cgcgcccgga caggttgacc agcaggaggg tgtcctttgt
     3361 cagcgccggc gcgattttcg cggcgtacgc gaccgcatgc gccgattcca gcgccgggat
     3421 gatcccctcg taccggcaca ggtcgtcaaa ggctttcagc gcctcgtcgt ccgtgacggc
     3481 gacgtactcg gcacgatgct gatccttgag ccacgcgtgc tccgggccga cgccggggta
     3541 atcgaggccc gccgataccg aatgcgtctc gacgatctgg ccgttggcgt cctgcagcag
     3601 gtaggtgcgg ttgccgtgca gcacgccggg cgtaccggcg caaagcgatg ccgcatgctt
     3661 gccggtatgc agtccctcgc ccgccgcctc gacgccgatc agccgtacct tctcttctcc
     3721 gatataggga tagaagatgc ccatggcgtt cgaaccgccg ccgacgcagg cgatcacggc
     3781 gtcgggctgg cgcccggcga gctcgggcat ctgccgctgg cactcctcgc cgatcaccga
     3841 ctggaaatcg cgcaccatca tcgggtacgg atgcggtccg gccaccgtgc cgatgatgta
     3901 gaaggtgtcc tcgacgttcg tgacccagtc gcgcatcgcc tcgttgagcg cgtccttgag
     3961 cgtcttcgag cccgactcga ccggcaccac ggtcgccccg agcagcttca tgcggtacac
     4021 gttctgcgcc tggcgcttga tgtcctcgga acccatgtag accacgcagg gcatgcccag
     4081 gcgggccgcc accgtggccg aggcgacccc gtgcatgccc gcgccggtct ccgcgatgat
     4141 gcgcttcttg cccatgcgcc gcgcgagcag cgcctggccg atggtgttgt tgatcttgtg
     4201 cgcgccggtg tggttcaggt cctcgcgctt gaggtagatc tgcgcgccgc cgaggacctg
     4261 cgaccagcgc ctggcgtggt agatcgggct cggccggccg acgtagtgct tgagctcgta
     4321 gcggaattcg gcgacgaact ccgggtcgcg gcgatagcgc tcgtaggcgt cgcgcagctc
     4381 ctcgagcgca tggctgagcg tctcggcgac gaacacgccg ccgtaggggc cgaagtgccc
     4441 gcgcgcgtcg ggcaagtcat gcatccgcat tcctgacctc cgcgatgaac gccgcgattt
     4501 tcgccgcgtc cttgatgcct ttcgccgact ccacgccgct cgacacgtcc accgcccagg
     4561 gccgcaggcg tcgcacggcc tgacccacgg tgccgctggc gagccccccc gagagcacca
     4621 gcgggcgctc gagccgcgcc ggcaccagcg accagtcgaa gcccgcgccg accccgccgt
     4681 actccgccac gtgcgcgtcg agcagccagc cgctcgctgc ggagaacggc tgcaggtatt
     4741 ttagcaaatc gacccccggc gccacgcggc aggccttgag gtacggcagg ccgaattgcg
     4801 cgcagaactc gggcgtttcc tcgccatgga actgcagcat cgccggatgc aggcgcccga
     4861 tcacctgcgc cacctgcgcg gcgtccggat tcacgaacaa cgccaccgcc tgcacgaacg
     4921 gcgggagcgc ggcgcggatc tcgcgggccc gctccacgct gaggaagcgc gggctgggcg
     4981 ggtagaacac caggccgatg gcgtccgccc ccgcacgcgc cgccgcctcc gcatctcctg
     5041 ggcgcgtgat gccgcagatc ttgacgcgcg tcctcaaggc agcaccggaa tgcgctgtgc
     5101 gggactcgct tgctgcggca ggccccagct cgccgcgtag gccacgcgct cgaggtaaag
     5161 cccctgcgcg gggaaggtcg gcgcggcgcg cgcgcggtcg cgcgcgtcga gcaccgcgcc
     5221 tgcccaggcc ggcggctgct tgcccttgcc cacggtcacc agcgtgccga cgatgttgcg
     5281 caccatgtgg tgaaggaacg cgttcgcgcg cagcacgaag tcgatgcgct cgccgcgcgt
     5341 ctcgatatcc agtgccagca gcgtgcgcac cggcgatttc gcctggcact cggcggagcg
     5401 gaacgaggaa aaatcgtgct cgcccaccaa gtggcgtgcc gccgcgcgca tcgccgccac
     5461 gtccagaggc aggtggaacc agccggcgtg gcgcgccgcc aatgccggcc gcaccgcgcg
     5521 gttgatgagc acgtagcggt aggagcgctc gcgcgcgccg tagcgcgcgt ggaattccgg
     5581 cggcacttcc tgcgcccact gcaccgccac cgattcgggg agcagcgcgt tcgcgccccg
     5641 tacccaggcg ctcccgggcc gcgccgcgcc ggtgtcgaag tgcaccacct gcccgagggc
     5701 gtgcacgccc cggtcggtgc gccccgcgca ggtgacgccg atgcgctcgc cggcgatcgc
     5761 gcccagcgcc tcctgcaggg cgtcctgcac cgtgccgccg ccaggctgcg tctgccagcc
     5821 caggaagcgg ctgccgtcgt attccagtgc cagagcgatt ctcatgccca tgaaatgacg
     5881 gagccacggc ccacggcgtc atgcgcgcgg cagccgtggc ccgatgtgtg gcgcgcgccc
     5941 ggacctagcc cagcttggcg agcatctgat tggcctggga ctgctgcgcg ccgtcgcctt
     6001 cccgcaccac ctcgttcagc aattcgcgcg cgccgtcctt gtcgcccatt tcctcgtagg
     6061 ccttggcgag gtcgagcttg gtcgc
//
LOCUS       DASRIE010000111        11466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1178051, whole genome shotgun sequence.
ACCESSION   DASRIE010000111 DASRIE010000000
VERSION     DASRIE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178051"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFB53_04925"
     CDS             <1..289
                     /locus_tag="VFB53_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HZN86115.1"
                     /translation="GAEPSVVLNKVLESKGNNGFNAQTEEYGDLVQQGVIDPTKVSRT
                     ALQNASSVAGLMLTTDAMVAELVEDKKGGGGHGHGDMGGMGGMGGMGGMDM"
     gene            399..474
                     /locus_tag="VFB53_04930"
     tRNA            399..474
                     /locus_tag="VFB53_04930"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:432..434,aa:Phe,seq:gaa)
     gene            496..1740
                     /gene="hemA"
                     /locus_tag="VFB53_04935"
     CDS             496..1740
                     /gene="hemA"
                     /locus_tag="VFB53_04935"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZN86116.1"
                     /translation="MTLFALGLNHTTAPLPVRERVVFHVEKLLGALGELTRGKEVAEA
                     AILSTCNRTELYLSAERPEAAAQWLAEYHRISPAEISPYLYTLPQDQAVRHTFRVAAG
                     IDSMVLGEPQILGQMKEAVRAAQAAGTLGTVLHKLFQRTFAVAKEVRSTTQLGANSVS
                     MAAAAVKLAARIFPSLKDQSVLFIGAGEMVELCATHFAAQAPARLTVANRTLERGQAL
                     AHRFNGRAIELRALPEQLQEHDIVVSCTASSLPILGKGLVERALRARKRRPMFMVDLA
                     VPRDIEAEVGELDDVFLYTLDDLQGIVRGNLDARRASLEQAEAIIETQVGQFMHWMSA
                     RAAVPLIRQLREQGEAARRQELERALRLLQRGEDPRQVLEALSQGLTNKLLHGPTQAL
                     NDAADEERRALAALVERLYRGK"
     gene            1737..2822
                     /gene="prfA"
                     /locus_tag="VFB53_04940"
     CDS             1737..2822
                     /gene="prfA"
                     /locus_tag="VFB53_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZN86117.1"
                     /translation="MKPALRTKLDSLVGRLGELERLLAAEDATRNLDQFRKLSREHAE
                     LAGLAALYERFRQAERDAAEAKAMAGDAAMKGYAEEELKNSRAAMDSLEAQLQKQLLP
                     RDPNDERNLFLEVRAGTGGDESALFAGDLFRMYSRYAERRGWQVEIISESVPEMGGYK
                     EVIARLIGQGAYSRLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADAIEDVVLN
                     PAELRVDTFRASGAGGQHVNKTDSAIRVTHLPTGIVVECQDSRSQHKNREKALSVLAA
                     RIKDKQTREQQAKTASTRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKIERIMD
                     GEIDELVDALSAEHQAEQLAQLAEEAA"
     gene            2839..3633
                     /gene="prmC"
                     /locus_tag="VFB53_04945"
     CDS             2839..3633
                     /gene="prmC"
                     /locus_tag="VFB53_04945"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZN86118.1"
                     /translation="MKVAEALKALEAREARLLLARATGFSEASVLAHPERELPAEAEA
                     AFRDFAARRALGEPIAYILGEKEFYGLPLAVNPAVLIPRPETELLVERALALRPASVL
                     DLGTGSGAVALAIKRQLASARVVAVERSAAALAVAQRNAMKLGLEVDFHHGPWFAPVA
                     GERFDLIVSNPPYVAAGDLHLLQGDLRFEPRTALVSGADGLEAIREIAAGAAAHLNPG
                     GWLLLEHGMGQDEDVRKLLLAQGLEGIESWPDLAGIARVTGGGLKS"
     gene            3637..3960
                     /gene="grxD"
                     /locus_tag="VFB53_04950"
     CDS             3637..3960
                     /gene="grxD"
                     /locus_tag="VFB53_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZN86119.1"
                     /translation="MDTQERIKQQVTGNPVVLYMKGTPQMPQCGFSAMAIQVLQACGV
                     NAPFTVNVLADPEIRQGIKEYANWPTIPQLYVNGEFIGGSDILREMYQTGELQKLLEG
                     LRQQA"
     gene            complement(3957..4880)
                     /locus_tag="VFB53_04955"
     CDS             complement(3957..4880)
                     /locus_tag="VFB53_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN86120.1"
                     /translation="MDRFHRMAVYVAVAEEESFAGGARRLGMSPPAVTRAIASLEKHL
                     GVKLLTRTTRFVRATDAGQRYLEQARRIIAEADEADEAVAGVNAAPRGRLAVTAPVLF
                     GRMFVTPSIVEYLERFPGMSIAAAFLDRVVNLLEEGMDVGVRIGELRDSSMKAIGVGH
                     VRRVVCAAPDYLKTRGMPRTPADLAKHTVIAANPASPSVDWKFTTDRKTTSVKLAPRL
                     TVTSNEAAIVAAVQGFGVTRLLSYQVAAHLVSGELQAVLTDYEPPRLPINVLHSEGRQ
                     ASAKVRTFVDLLVDRLRADKALNRSGARSAA"
     gene            5023..5634
                     /locus_tag="VFB53_04960"
     CDS             5023..5634
                     /locus_tag="VFB53_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN86121.1"
                     /translation="MTRQTLVPSDVAFTPAVKAIQARNGSRGAYARMEEGEGWETTLT
                     PEVAAFIAAQTSVFLGTANAAGQPYIQHRGGPAGFLRVLDEKTIGFADLRGNRQFITL
                     GNLAENPRAHLFLIDYAQRRRVKIWGEARVVEDQPALLEKLVPASEKSRAERAIMFSV
                     AAWDANCPKHIPQRFEAADVARALEERDRRIAELEKELARLTQ"
     gene            complement(5648..6949)
                     /locus_tag="VFB53_04965"
     CDS             complement(5648..6949)
                     /locus_tag="VFB53_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN86122.1"
                     /translation="MSETTLIPVPERIGKYPVLKELGRGATSVVYLCNDPFYARNVAV
                     KVVMPEAFADKDRGNLYRKIFVTEASLAGKLQHPHICQIYDAVADDRQQYIVMEYVDG
                     GTLERHGDAQDLLPIERVVEIIFKCTRALEFAQKMGITHRDLKPANILVTGETDVKVS
                     DFGAALIASGDNTQISAIGSPAYMSPEQVKEHTLNHQTDIYSLGVVLYQLLAGRLPFQ
                     ATNNFSLIYQIANVEPQPPSSYRPQIPAALDAIVKRAMAKDLAQRYASWEDFSLDLAE
                     VFRNEGLGGRTQKFADSDKFESLRKLPFFANFTDAELWEVARISNWRGAQAGELLMKE
                     GEPGNFFCLLAEGEVRVTRKGKLLNILHAGECFGEMAYLSKDEHLRGADVTVMADSKI
                     VSVPTQKLAQASDTCRHKFDRAFMEILVERLTMANARLSGV"
     gene            complement(6949..8382)
                     /gene="argH"
                     /locus_tag="VFB53_04970"
     CDS             complement(6949..8382)
                     /gene="argH"
                     /locus_tag="VFB53_04970"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZN86123.1"
                     /translation="MAPPRSKRPPRTKAGSPAPWSDRFVEPVSDLVKRYTASVGFDQR
                     LAAHDIRASLAHARMLAACGVIARADCAAIERGLAQVAGEIETGAFAWSEDAEDVHGN
                     IERRLIALVGEAGKRLHTARSRNDQVATDVRLWLRDEIDRVDALIARLQEALLEQAAR
                     HAALVMPGFTHLQVAQPVTFGHHLMAYFEMLERDRQRFADCRRRVNLLPLGAAALAGT
                     SFPIDRERVARELGFDGLCANSLDAVSDRDFAIEFCAHAALAMVHLSRFAEDLVLWMS
                     PRFGFVRMPDRFTTGSSIMPQKKNPDVPELARGKSARVIGDLTALLALMKGQPLAYNK
                     DNQEDKEPLFDAVDTLQNTLAVFAEMVPGIEPAPEAMRAAALEGFATATDLADYLVRK
                     GVAFKDAHAIVARAVREAEGVGCDLSGLPLAVLKRFSPAIGDDVYHSLSLEGSVAARD
                     HAGGTAPKRVRAAVARARSRRGKADPA"
     gene            8422..9432
                     /locus_tag="VFB53_04975"
     CDS             8422..9432
                     /locus_tag="VFB53_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN86124.1"
                     /translation="MSIKQNFVTDTLPDFRNLGVIARILLGVNAFAFAVALAAAPRWE
                     AAQEAFMRGATLLEPILLATLVPLYALAPLLSRLRYWLGCVAVLALVLAASGGTHAAL
                     AAAQIAPDEGLARALGLAALAAAALLAYLRLLTKAYSPALTEARLQALQSRIRPHFLF
                     NSLNAVLSLIRRDPARAEHALEDLADLFRVLMQDNRSFVRLSDEIALLQRYARLEQLR
                     LGERLEIDWQLGTAPLQALLPPMVLQPLLENAVYHGVEPGTGTGRVAVRIERRGARVL
                     ARIENPYLEEHAQRAGNRMALANIRERLQLFFDAEARLEARVAGERYLVEIEMPYREA
                     PA"
     gene            9429..10187
                     /locus_tag="VFB53_04980"
     CDS             9429..10187
                     /locus_tag="VFB53_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN86125.1"
                     /translation="MSALRVFIADDEAPARERLRELLADLAAELPTEVVGEAPNGLEA
                     AARLPDSGAQVLLLDVEMPGMGGLELARHLAGLAAGPAVVFVTAHDRHAVEAFELNAL
                     DYLVKPVRAARLAAALKKATAAGPPPRGQLARAAAQPREYLSVAERNRILLVPVRDIV
                     YLRAELKYVTLRTRAGEHLIEEPLVALEREFAGRFVRVHRNCLVARSAVRGFERAAGG
                     DEESHWNVVLEGVAERVPVSRRQWAEVRTLAKEA"
     gene            10187..11101
                     /gene="hemC"
                     /locus_tag="VFB53_04985"
     CDS             10187..11101
                     /gene="hemC"
                     /locus_tag="VFB53_04985"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN86126.1"
                     /translation="MRLRIATRRSRLALWQAGHVRERLEALHPGLAVELLPLSTRGDE
                     LLDERLDKVGGKGLFVKELESAMAEGRADLAVHSMKDVPAQLPEGYALAAILEREDPR
                     DAFVSGRYAALAELPPGARVGTSSLRRAAQVAARHPGLQLTTLRGNVETRLAKLDRGE
                     YDAILLAAAGLKRLGLAARIRASLPVEDCLPAPGQAALGIECLAADARVLGLVAPLAH
                     AATSACVRAERAVSLALGGSCTIPLGAFAEIAGGELRLRALVAAPDGRRSAHADCAGP
                     AADPEALGARAAQLLRARGADEILAAIG"
     gene            11104..>11466
                     /locus_tag="VFB53_04990"
     CDS             11104..>11466
                     /locus_tag="VFB53_04990"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN86127.1"
                     /translation="MALAGKRILVTRPAELAGGLAALIREAGGEPVLYPAIEIRDPED
                     PAAARALLARLPEFELAVFVSPSAVRKALELAPRGPWPRVAAVGEGTRRALEERGFRD
                     VIAPAGGADSEALLEVLAR"
BASE COUNT         1671 a         4024 c         4135 g         1636 t
ORIGIN      
        1 cggcgccgag ccgtcggtgg tcctcaacaa ggtcctcgag agcaagggca acaacggctt
       61 caacgcccag accgaggagt acggcgatct cgtgcagcag ggcgtgatcg acccgaccaa
      121 ggtgagccgc accgcgctgc agaacgcctc ctcggtcgcc ggcctgatgc tcaccaccga
      181 tgcgatggtg gccgagctgg tcgaggacaa gaagggcggc ggcggccatg gccacggcga
      241 catgggcggc atgggcggga tgggtggcat gggcggcatg gacatgtaac taacagaggg
      301 aaacctaggt ttccctccgt tacctccctt ccgctacaca gtcgttcggg ggcgcttcgg
      361 cgcccctttt cttttataat ccgcgctctt ttccggcagg ccaagtagct cagttggtag
      421 agcagcggac tgaaaatccg tgtgtcgctg gttcgattcc ggccttggcc accatttcat
      481 tagaatgacc tgcaggtgac gctcttcgcc ctcggcctca atcacaccac cgcgccgctg
      541 ccggtgcgcg agcgcgtggt gttccacgtc gagaagctgc tcggcgcgct gggcgagctg
      601 acgcgcggca aggaggtcgc cgaggcggcg atcctctcca cctgcaaccg caccgagctc
      661 tacctgtcgg cggagcggcc ggaagcggcc gcgcagtggc tggccgagta ccaccgcatc
      721 agccccgccg agatctcccc ctacctctac accctgccgc aggaccaggc ggtgcgccac
      781 accttccgcg tcgccgccgg catcgactcg atggtgctgg gcgagccgca gatcctcggc
      841 cagatgaagg aggcggtgcg cgcggcgcag gccgccggca cgctgggcac ggtgctgcac
      901 aagctgttcc agcgcacctt cgcggtggcc aaggaagtgc gcagcaccac gcagctcggc
      961 gccaacagcg tgtcgatggc cgccgccgcg gtgaagctcg ccgcgcgcat cttccccagc
     1021 ctgaaggacc agtcggtcct gttcatcggc gccggcgaga tggtcgagct gtgcgccacg
     1081 cacttcgccg cgcaggcgcc ggcgcggctc acggtggcca accgcacgct ggagcgcggc
     1141 caggcgctgg cgcaccgctt caacggccgc gccatcgagc tgcgcgccct gcccgagcag
     1201 ctgcaggaac acgacatcgt ggtgtcctgc acggcatcct cgctgccgat cctgggcaag
     1261 ggcctggtgg agcgcgcgct gcgcgcgcgc aagcgccggc cgatgttcat ggtggacctg
     1321 gcggtgccgc gcgacatcga ggccgaggtg ggcgagctgg acgacgtgtt cctctacacc
     1381 ctggacgacc tgcagggcat cgtgcgcggc aacctcgacg cgcgccgcgc ctcgctggag
     1441 caggccgagg cgatcatcga gacgcaggtc ggccagttca tgcactggat gagcgcgcgc
     1501 gccgcggtgc cgctgatccg ccagctgcgc gagcagggcg aggcggcgcg ccgccaggag
     1561 ctggagcgcg ccctgcgcct gctgcagcgg ggcgaggacc ccaggcaggt gctcgaggcg
     1621 ctgtcgcagg gcctcaccaa caagctgctg cacgggccga cgcaggcgct caacgacgcc
     1681 gccgacgagg agcgccgcgc gctcgccgcg ctcgtcgagc gcctgtaccg cggcaagtga
     1741 aaccggcgct ccgcaccaag ctcgacagcc tggtcgggcg cctgggcgag ctcgagcgcc
     1801 tgctcgcggc ggaggacgcc acgcgcaacc tggaccagtt caggaagctc tcgcgcgagc
     1861 acgcggagct cgccggcctc gccgcgctgt acgagcgctt ccgccaggcc gagcgcgacg
     1921 ccgccgaggc gaaggccatg gcgggcgacg cggcgatgaa gggctacgcc gaggaggagt
     1981 tgaagaactc gcgcgcggcg atggacagcc tcgaggccca gctgcagaag cagctcctgc
     2041 cgcgggaccc caacgacgaa cgcaacctgt tcctcgaggt gcgcgccggc accggcgggg
     2101 acgaatcggc cctcttcgcc ggcgacctgt tccgcatgta ctcgcgctac gccgagcgca
     2161 ggggctggca ggtggagatc atctccgaga gcgtccccga gatgggcggc tacaaggagg
     2221 tgatcgcgcg cctcatcggc cagggggcct attcgcgcct caagttcgag tcgggcggcc
     2281 accgcgtgca gcgcgtgccc gagaccgagg cgcagggacg catccacacc tcggcctgca
     2341 cggtggcggt gctgcccgag gccgacgcca tcgaggacgt ggtgctcaac ccggccgagc
     2401 tgcgcgtgga caccttccgc gcctcgggcg cgggcggcca gcacgtgaac aagaccgact
     2461 cggcgatccg cgtcacgcac ctgcccaccg gcatcgtggt ggagtgccag gattcgcgct
     2521 cgcagcacaa gaaccgcgag aaggcgctct cggtgctggc ggcgcgcatc aaggacaagc
     2581 agacgcgcga gcagcaggcg aagaccgcct cgacgcgcaa gtcgctggtg gggtcgggcg
     2641 accgctccga gcgcatccgc acctacaact tcccgcaggg ccgcgtcacc gaccaccgca
     2701 tcaacctcac gctgtacaag atcgagcgca tcatggacgg cgagatcgac gagctggtgg
     2761 acgcgctctc ggccgagcac caggccgagc agctggcgca gctggcagaa gaggcggcat
     2821 gaatgccgcg gaagcggctt gaaagtcgcc gaagcgctca aggcgctgga agcgcgcgag
     2881 gcgcggctgc tcctcgcgcg cgccaccggc ttctccgaag cgagcgtgct cgcgcacccg
     2941 gagcgcgagc tgcctgccga ggccgaagcc gccttccgcg atttcgccgc gcgccgcgcg
     3001 ctcggcgagc cgatcgccta catcctcggc gagaaggagt tctacggcct gccgctggcg
     3061 gtcaatcccg ccgtgctgat ccccaggccg gagaccgagc tcctcgtgga gcgagcgctg
     3121 gcgctgcggc cggcttcggt gctcgacctg ggcaccggct cgggcgcggt ggcgctcgcg
     3181 atcaagcggc aactcgcctc cgccagggtc gtcgccgtgg aacgcagcgc cgcggcgctc
     3241 gcggtggcgc agaggaacgc gatgaagctc gggctggaag tggatttcca ccacggcccc
     3301 tggttcgcgc cggtggcggg ggagcgcttc gatctcatcg tctccaatcc cccttacgtc
     3361 gccgcgggtg atctgcacct cctgcagggg gacctgcgct tcgagccgcg cacggcgctg
     3421 gtgtccggcg cggacgggct ggaggcgatc cgcgagatcg ccgcgggcgc ggccgcgcac
     3481 ctcaatccgg gcggctggct gctcctcgag cacggcatgg gccaggacga ggacgtgagg
     3541 aagctgcttc tcgcgcaggg gcttgaaggg atcgagagct ggcccgatct ggccggcatt
     3601 gcccgggtca cgggcggagg gctaaaatcc taggttatgg acacccaaga acgcatcaag
     3661 caacaagtca ccggcaaccc ggtggtgctg tacatgaagg gcacgccgca gatgccgcag
     3721 tgcggcttct cggcgatggc gatccaggtg ctgcaggcct gcggggtgaa tgcgcccttc
     3781 accgtgaacg tgctcgccga cccggagatc cgccagggca tcaaggaata cgccaactgg
     3841 ccgaccattc cgcagctcta cgtcaacggc gagttcatcg gcggctcgga catcctgcgc
     3901 gagatgtacc agaccggcga gctgcagaag ctgctcgagg ggctcaggca acaggcttag
     3961 gccgcgctgc gcgcgcctga tcgattcagc gccttgtccg ctcgcagcct gtccaccagc
     4021 aagtcgacga atgtcctgac cttcgcggat gcctgccggc cttcgctgtg caggacattg
     4081 atcggcaggc gcggcggctc gtagtcggtc agtaccgcct gcaactcacc cgacaccagg
     4141 tgcgcggcga cctggtagga cagcaggcgt gtgacgccga aaccctgtac cgctgccacg
     4201 atggcggcct cgttgctcgt aaccgtcagc cgcggcgcga gcttgacgct cgtcgtcttc
     4261 ctgtcggtcg tgaatttcca gtcgacggaa ggcgacgccg gattcgcggc aatcacggtg
     4321 tgcttggcga gatcggcggg cgtgcgcggc atgcccctgg tcttgaggta atccggcgcg
     4381 gcgcacacca cgcggcggac atggccgacg ccgatcgcct tcatgctcga gtcgcgcagc
     4441 tcgccgatgc gcacgcccac gtccatgcct tcctcgagca ggttgacgac ccggtccagg
     4501 aaggccgccg cgatggacat gccgggaaag cgctccagat attcgacgat gctgggggta
     4561 acgaacatgc ggccgaacag tacgggcgcg gtgaccgcca gccgtccgcg cggcgcggca
     4621 ttcaccccgg cgaccgcttc atcggcttcg tccgcttccg cgatgatgcg ccgggcctgc
     4681 tccaggtaac gctgacccgc atcggtggcc cggacgaacc gggtggtccg ggtgagcagc
     4741 ttcacgccga gatgtttctc gagtgatgcg atcgcccggg tcactgcggg cggggacatc
     4801 cccagcctgc gcgcgccgcc ggcgaagctc tcttcttccg ccaccgccac ataaacggcc
     4861 atgcgatgga accggtccat gcatcattcc agttgtcgga agaatgaatt gtaatcggcg
     4921 acgattgttc ttttcaacgg aattctccaa gatgcgtccc gtccgcgacg tattcggcgc
     4981 tcctgtcctc gagtcgcggc gcgcggcctc aaaggagtcg tcatgacccg gcaaacgctc
     5041 gtccccagcg atgtcgcatt cacaccggcc gtgaaggcca tccaggcgcg caacggctcg
     5101 cgcggggcgt acgcccggat ggaggaaggc gagggctggg aaaccacgct cacgccggag
     5161 gttgccgcgt tcatcgccgc gcagaccagc gtattcctcg gcacggcgaa tgccgccggt
     5221 cagccgtaca tccagcatcg cggcggcccg gcgggatttc tgcgcgtcct cgatgaaaag
     5281 acgatcggct tcgcggatct tcgcggcaac cggcaattca tcacgctggg caacctcgcg
     5341 gaaaacccca gggcccacct gttcctgatc gactacgcgc agcgccggcg cgtgaagatc
     5401 tggggcgagg cgcgcgtggt ggaggaccag cccgcgttgc tggaaaagct cgtgcccgcg
     5461 agcgaaaagt cgcgggccga gcgggcgatc atgttcagcg tggcggcgtg ggatgcgaac
     5521 tgcccgaagc acattccgca gcgcttcgaa gcagcggacg tggcgcgcgc gctcgaggag
     5581 cgcgaccggc gcattgccga actcgaaaag gagctcgcgc gcctgacgca gtgacggcgc
     5641 agggcgttca gacgcccgac agccgggcgt tggccatggt caggcgctcg accaggatct
     5701 ccatgaaggc gcggtcgaac ttgtggcggc aggtgtcgga ggcctgcgcc agcttctgcg
     5761 tcggcaccga aacgatcttg gagtcggcca tcaccgtgac gtcggcgccg cgcaggtgct
     5821 cgtccttgga cagataggcc atctcgccga agcactcgcc ggcgtgcagg atgttgagca
     5881 gcttgccctt gcgcgtcacg cgcacctcgc cctcggcgag caggcagaag aagttgcccg
     5941 gctcgccctc cttcatcagc agctcgcccg cctgcgcgcc gcgccagttg gagatgcgcg
     6001 ccacctccca cagctcggcg tcggtgaagt tggcgaagaa cgggagcttg cgcagcgact
     6061 cgaacttgtc cgagtcggcg aacttctgcg tcctgccgcc caggccctcg ttcctgaaca
     6121 cctccgccag gtcgagcgag aagtcctccc agctggcgta gcgctgcgcc aggtccttgg
     6181 ccatggcgcg cttcacgatc gcgtcgagcg ccgccgggat ctgcgggcgg taggaggagg
     6241 gcggctgcgg ctcgacgttg gcgatctggt agatcaggct gaagttgttg gtggcctgga
     6301 acggcagccg cccggccagc agctggtaca gcaccacgcc gagcgagtag atgtcggtct
     6361 ggtggttgag ggtgtgctcc ttcacctgct cgggcgacat gtaggccggc gagccgatgg
     6421 ccgagatctg cgtgttgtcg ccgctggcga tgagcgcggc gccgaagtcc gacaccttga
     6481 cgtcggtctc gccggtgacc aggatgttgg cgggcttcag gtcgcggtgc gtgatgccca
     6541 tcttctgcgc gaactccagc gcgcgcgtgc acttgaagat gatctccacc acgcgctcga
     6601 tgggcagcag gtcctgcgcg tcgccgtggc gctcgagggt cccgccgtcg acgtactcca
     6661 tcacgatgta ctgctgccgg tcgtcggcca ccgcgtcgta gatctggcag atgtgcgggt
     6721 gctgcagctt gccggcgagc gaggcctcgg tgacgaagat cttgcggtac aggttgccgc
     6781 ggtccttgtc ggcgaaggcc tccggcatca ccaccttgac ggcgacgttg cgcgcgtaga
     6841 acgggtcgtt gcacaggtac accacgctgg tggcgccgcg ccccagctcc ttcagcaccg
     6901 ggtacttgcc gatgcgctcc ggcaccggga tcagcgtggt ctcgctcatc atgccgggtc
     6961 cgccttgccg cgccggctgc gcgcgcgcgc caccgccgcc ctgacgcgct tcggggcggt
     7021 gccgccggcg tggtcgcgcg ccgccaccga gccctccagc gagaggctgt ggtacacgtc
     7081 gtcgccgatc gccggcgaga agcgcttgag caccgcgagc ggcaggcccg agaggtcgca
     7141 gcccacgcct tcggcctcgc gcaccgcgcg cgccacgatg gcgtgcgcat ccttgaacgc
     7201 cacgcccttg cgcaccaggt agtcggcgag gtcggtggcg gtggcgaagc cctccagcgc
     7261 cgcggcgcgc atcgcctcgg gcgccggctc gattcccggc accatctcgg cgaacacggc
     7321 gagcgtgttc tgcagcgtgt ccacggcgtc gaacagcggc tccttgtcct cctggttgtc
     7381 cttgttgtag gcgagcggct ggcccttcat cagcgcgagc agtgccgtga ggtcgccgat
     7441 cacgcgcgcg ctcttccccc gcgccagctc cggcacgtcg gggttcttct tctgcggcat
     7501 gatcgagctg ccggtggtga agcggtcggg catcctcacg aagccgaagc gcgggctcat
     7561 ccacagcacc aggtcctcgg cgaagcgcga caggtgcacc atggcgagcg cggcgtgcgc
     7621 gcagaattcg atggcgaagt cccggtcgga caccgcatcc agcgaattgg cgcacagccc
     7681 gtcgaagccc agctcgcgcg ccacgcgctc gcggtcgatg gggaagctgg tgccggcgag
     7741 cgccgctgcg cccagcggca gcaggttgac gcgccgccgg cagtcggcga agcgctgccg
     7801 gtcgcgctcc agcatctcga agtaggccat caggtggtgg ccgaaggtca ccggctgcgc
     7861 cacctgcagg tgcgtgaagc ccggcatcac cagcgcggcg tggcgcgcgg cctgctcgag
     7921 cagcgcctcc tgcagccggg cgatgagcgc gtcgacgcgg tcgatctcgt cgcgcagcca
     7981 cagccgcacg tcggtcgcca cctggtcgtt cctcgagcgc gcggtgtgca ggcgcttgcc
     8041 ggcctcgccc accagcgcga tcaggcgccg ctcgatgttg ccgtgcacgt cctcggcatc
     8101 ctccgaccag gcgaaggcgc cggtctcgat ttccccggcc acctgcgcca ggccgcgctc
     8161 gatcgcggcg cagtcggcgc gcgcgatgac gccgcaggcg gcgagcatgc gcgcgtgcgc
     8221 gagggaggcc cggatgtcgt gcgcggcgag gcgctggtcg aagcccaccg acgcggtgta
     8281 gcgcttcacc aggtcgctga ccggctcgac gaaccggtcg gaccacggtg ccggcgagcc
     8341 ggccttagtg cgcggcggcc gcttggagcg aggaggggcc atggggcata ctcgtgaccc
     8401 gcggcgcgcc gcggtatgcc aatgagtata aagcaaaact tcgtcaccga taccctgccg
     8461 gatttccgca acctgggggt catcgcccgc atcctgctcg gcgtcaacgc gttcgctttc
     8521 gccgtcgcgc tggccgcggc gccgcgctgg gaagcggcgc aggaggcgtt catgcgcggc
     8581 gcgacgctgc tcgagccgat cctgctcgcc accctggttc cgctctacgc gctcgcgccg
     8641 ctgctgtcgc ggctgcgcta ctggctgggc tgcgtggcgg tgctggcgct ggtgctcgcc
     8701 gcgagcggcg gcacgcatgc cgcgctcgcc gcggcgcaga ttgcgcccga cgagggcctg
     8761 gcgcgcgccc tggggctggc ggcgctggcg gcggcggcgc tgctcgccta cctgcggctg
     8821 ctgaccaagg cctactcgcc ggcgctcacc gaagcgcggc tgcaggcgct gcagtcgagg
     8881 atccgcccgc acttcctgtt caacagcctg aacgcggtgc tgtcgctgat ccgccgcgat
     8941 cccgcgcgcg ccgagcacgc gctcgaggac ctcgcggacc tgttccgcgt gctgatgcag
     9001 gacaaccgca gcttcgtgcg gctgtccgac gaaatcgcgc tgctgcagcg ctacgccagg
     9061 ctcgagcagc tgcgcctggg cgagcgcctg gagatcgact ggcagctggg cacggcgccg
     9121 ctgcaggcgc tgctgccgcc gatggtgctg cagccgctgc tggagaacgc ggtgtaccac
     9181 ggcgtcgagc cgggcaccgg caccgggcgc gtggcggtgc gcatcgagcg gcgcggcgcg
     9241 cgggtgctcg cgcgcatcga gaatccctac ctcgaggagc atgcgcagcg cgccggcaac
     9301 cgcatggcgc tggccaacat ccgcgagcgg ctgcagctgt tcttcgacgc cgaggcgcgg
     9361 ctggaggcgc gcgtcgccgg cgagcgctac ctggtggaga tcgagatgcc ctaccgcgag
     9421 gcgccggcgt gagcgcgctg cgcgtgttca tcgccgacga cgaggccccg gcgcgcgagc
     9481 gcctgcggga gctgctcgcc gatctcgccg cggagctgcc caccgaggtg gtgggcgagg
     9541 cgcccaacgg cctggaggcc gccgcgcggc tgcccgacag cggcgcccag gtcctgctgc
     9601 tcgacgtcga gatgcccggc atgggcgggc tggagctggc gcggcacctc gccggcctgg
     9661 cggccggccc tgcggtggtg ttcgtcaccg cgcacgaccg gcacgcggtg gaggccttcg
     9721 agctcaacgc gctcgactac ctggtcaagc cggtgcgcgc cgcgcgcctg gcggcggcgc
     9781 tgaagaaggc caccgccgcg ggaccgccgc cgcgcggcca gctggcgcgc gccgcggcgc
     9841 agccgcgcga gtacctgtcg gtggccgagc gcaaccggat actgctggtg ccggtgcgcg
     9901 acatcgtcta cctgcgcgcc gagctgaaat acgtcacgct gcgcacgcgc gccggcgagc
     9961 acctgatcga ggagccgctg gtggcgctgg agcgggagtt cgccgggcgc ttcgtgcgcg
    10021 tgcaccgcaa ctgcctggtg gcgaggagcg cggtgcgcgg cttcgagcgc gccgccggcg
    10081 gcgacgagga atcgcactgg aacgtggtgc tggagggcgt ggccgagcgc gtgccggtga
    10141 gccgccggca atgggccgag gtgcgcaccc tggcgaagga ggcctgatgc gcctgaggat
    10201 cgccacgcgc cgcagccggc tggcgctgtg gcaggccggg cacgtccggg agcgcctcga
    10261 ggcgctgcac ccagggctgg cggtggagct gctgccgctc tcgacgcgcg gcgacgagct
    10321 gctcgacgag cgcctcgaca aggtgggcgg caagggcctg ttcgtgaagg agctcgagag
    10381 cgcgatggcc gagggccgcg ccgacctcgc ggtgcactcg atgaaggacg tgccggcgca
    10441 gctgccggag ggctacgcgc tcgccgcgat cctcgagcgc gaggacccgc gcgacgcctt
    10501 cgtctccggc cgctacgccg cgctcgccga gctgccgccg ggggcgcgcg tgggcacctc
    10561 cagcctgcgg cgcgcggcgc aggtcgccgc ccggcacccc ggattgcagc tgacgaccct
    10621 gcgcggcaac gtcgagacgc gcctcgccaa gctcgaccgc ggcgagtacg acgcgatcct
    10681 gctcgccgcc gccggcctga agcgccttgg cctcgccgcg cgcatccgcg ccagcctgcc
    10741 ggtggaggat tgcctgcccg cgccggggca ggcggcgctc ggcatcgagt gcctcgccgc
    10801 ggacgcccgc gtcctcggcc tggtggcgcc gctcgcgcac gcggcgacga gcgcctgcgt
    10861 gcgcgccgag cgcgcggtaa gcctggctct cggcggcagc tgcaccatcc cgctgggcgc
    10921 cttcgccgag atcgcgggcg gcgagctgcg cctgcgcgcg ctggtggccg cgcccgacgg
    10981 caggcgcagc gcgcacgccg attgcgccgg gcccgccgcc gacccggagg cgctgggcgc
    11041 gcgcgcggcg cagctgctgc gcgcgcgcgg cgccgacgag atcctggcgg cgatcggctg
    11101 agcgtggcgc tcgcgggcaa gcgcatcctc gtgacgcggc cggcggaact ggccggcggc
    11161 ctcgccgcgc tgatccgcga ggccgggggg gagccggtcc tctacccggc gatcgagatc
    11221 cgcgacccgg aagacccggc ggcggcgcgc gcgctcctcg cgcgcctgcc cgaattcgaa
    11281 ctcgcggtgt tcgtcagccc gagcgcggtg cgcaaggcgc tggagctcgc gccccgcggc
    11341 ccctggccgc gcgtcgccgc ggtgggcgag ggcacgcggc gcgcgctcga ggagcgcggc
    11401 ttccgggacg tgatcgcgcc cgcgggcggc gcggacagcg aagcgctgct cgaagtcctc
    11461 gcgcgg
//
LOCUS       DASRIE010000112         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3512167, whole genome shotgun sequence.
ACCESSION   DASRIE010000112 DASRIE010000000
VERSION     DASRIE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512167"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="VFB53_04995"
     CDS             complement(<1..2438)
                     /locus_tag="VFB53_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86128.1"
                     /translation="MIARIAGAFSVPLFVDLLGVPNLMWLSAAALALCLVFLVGVLPM
                     IVRRHDPSIDASKPHRARSLPEQFREVMGDPYLKLVTGIAVLAVFGKYFVDFAFLEQM
                     KARYSDAKHLAGFFGLFSGLTQGLSLLTRLFVSGPLLQRFGIRVGLLVLPLVHIVCTA
                     LIVLNGLSGEGGEGALVFWLVIANQGVYKTLKHPIDNPSFKVLYQPLKSEKRLAAQIS
                     VETIFTPITIGIAGAIMLLMSTVIPYDPVRFSLVLLLNFMLWAFVARLGSKAYQGALL
                     DVLRRRIVDDAPFTLNDATSVALLRGKLASDSPTEIMFALHLLERAEYPRTVDAMIEK
                     LDHSDAGVRRFALERLRALGATAAAPEVRRRLGLEREPRALAEALRSLGALGGEATLP
                     EIERHVDDPSPRVRRAALSALLARGSGEGFERALLRLGTLAASSQPAERLLLAQIAQA
                     LPAGHSDDWIAPLLNDGEPAVRREALQAAARSTSLRPRVIDHLGHAGFSGAAAAALAA
                     GGPDTAQALVERFTAGSKRALGIAIARVLGALGGDVAVAALRLRLDHPEEAVRTQVLE
                     ALVRVGYAAKRPDEQAAFDELLRNEAAHAALTLAARAGLPAALPAFAPLRQALEIEAN
                     EARRRAFLLLAIRYERAAILRARDNITSASRDRRAYASEVLDVTIEGRMRSVVLPLVE
                     DRSAEERLHALRSQFPQAERSPAEWLRTLLTRPEGQLRPWTRAWAARTSALTGEKALR
                     GELHDLRASDPMVRQTALAALDALSEVHDEWEGKPAMLLIEKVMILKGVQIFAQTSEE
                     VLTEIAAVVEELEKP"
BASE COUNT          377 a          896 c          853 g          328 t
ORIGIN      
        1 ggcttctcga gctcttccac caccgcggcg atctcggtga gcacctcctc ggaggtctgc
       61 gcgaagatct gcacgccctt caggatcatc actttctcga tcagcagcat ggctggcttt
      121 ccttcccact cgtcgtgcac ttcggacagc gcatcgagcg cggccaacgc cgtctgccgc
      181 accatcgggt cgctcgcgcg caggtcgtgc agctctccgc gcagcgcctt ctcgccggtc
      241 agcgcggagg tgcgcgcggc ccaggcccgc gtccacggcc ggagctgccc ttccgggcgc
      301 gtcagcagtg tgcgcagcca ctcggcgggc gatcgctcgg cctgcgggaa ctgcgagcgg
      361 agcgcgtgca gccgctcctc ggcggagcgg tcctcgacga gcggcagcac caccgagcgc
      421 attcggcctt cgatcgtcac gtcgagcact tcggacgcgt aggcgcgccg gtcgcgcgaa
      481 gcgctcgtga tgttgtcgcg cgcgcgcagg atcgcggccc gctcgtagcg gatcgccagc
      541 agcaggaacg cgcgccggcg cgcctcgttg gcttcgatct ccaaggcctg gcggagcggc
      601 gcaaaggcag gcagcgccgc gggcagcccc gcccgtgcgg cgagcgtcag cgccgcgtgc
      661 gcggcttcgt tgcgcaagag ctcgtcgaag gcggcctgct cgtcgggccg cttcgcggcg
      721 tagccgacgc gcaccagcgc ttccaggacc tgggtgcgca cggcctcttc cgggtgatcg
      781 agccgcaagc gcagcgccgc caccgccacg tcgccgccca aagcgcccag cacccgcgcg
      841 atcgcgatgc cgagcgcgcg cttgctgccc gccgtgaagc gctcgacgag cgcctgcgcg
      901 gtgtcggggc cgccggccgc gagcgcggcg gcggccgccc cggagaagcc ggcgtggccg
      961 aggtgatcga tcactcgcgg gcgcaacgac gtcgagcgcg cggccgcctg cagcgcctcg
     1021 cgtctcaccg ccggctcgcc gtcgttcaag agcggggcga tccagtcgtc ggagtgcccg
     1081 gcgggcagcg cctgcgcgat ctgggccaac agcagccgtt cagccggctg gctggaggcg
     1141 gccagcgtgc ccagccgcag cagcgcccgc tcgaagcctt cgccgctgcc gcgcgcgagc
     1201 agcgcggaga gcgcggcacg ccggacgcgt ggactcgggt cgtcgacatg gcgttcgatc
     1261 tcgggcagcg tcgcctcgcc gcccagcgct cccagcgagc gcaacgcctc ggcgagcgcg
     1321 cgcggctccc gttccaggcc caggcggcgc cgcacttcgg gcgcggcggc ggtcgcgccc
     1381 agcgcgcgca gccgctccag cgcgaagcgg cggacgccgg cgtccgagtg gtcgagcttc
     1441 tcgatcatcg cgtccaccgt gcgcgggtac tcggcgcgct cgagcaggtg cagcgcgaac
     1501 atgatctcgg tcggcgagtc gctggcgagc ttcccgcgca gcagcgcgac gctggtggcg
     1561 tcgttcagcg tgaacggagc gtcgtccacg atccgccgcc gcagcacgtc caggagcgcg
     1621 ccctggtacg ccttgctgcc gagccgcgcc acgaacgccc acagcatgaa gttcagcaat
     1681 agcaccagcg agaagcgcac cgggtcatac gggatcaccg tgctcatcag cagcatgatc
     1741 gcgccggcga tgccgatcgt gatcggcgtg aagatcgtct ccacgctgat ctgcgcggcc
     1801 agccgcttct ccgacttgag cggctggtag agcaccttga aggacgggtt gtcgatcggg
     1861 tgcttcaagg tcttgtagac gccctgattc gcgatcacca gccagaaaac gagcgcgccc
     1921 tcgccgccct cgccgctcaa accgttgagc acgatcaacg ccgtgcaaac gatgtggacg
     1981 agcggaagca ccagcaggcc gacgcggatt ccgaagcgct gcaggagcgg gcccgacacg
     2041 aagaggcgcg tcagcagcga caggccctgg gtcaaacccg agaacagccc gaagaacccg
     2101 gccaggtgct tggcgtcgct gtagcgcgcc ttcatctgct cgaggaaggc gaagtcgacg
     2161 aagtactttc cgaaaacagc cagcaccgcg atcccggtga cgagcttcag gtacgggtcg
     2221 cccatcacct cgcggaactg ctccggcagc gagcgcgccc ggtgcggctt gctggcgtcg
     2281 atgctgggat cgtggcgccg cacgatcatc ggcagcacgc cgaccaggaa caccaggcac
     2341 agcgcgagcg ccgcggccga cagccacatc aggttcggca cgccgagcag gtccacgaac
     2401 agcggcaccg agaaagcgcc ggcgatgcgc gcgatcacct ctccggctcc gatc
//
LOCUS       DASRIE010000113         9512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3238789, whole genome shotgun sequence.
ACCESSION   DASRIE010000113 DASRIE010000000
VERSION     DASRIE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238789"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="mraY"
                     /locus_tag="VFB53_05000"
     CDS             <1..767
                     /gene="mraY"
                     /locus_tag="VFB53_05000"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZN86129.1"
                     /translation="IVGWVDDYRKVVHRNPKGLSAKAKLFWQSLIGLAAACYLGYLSQ
                     RVPALNELIVPFFKHVAYPLGMVGFIVLTFFVIVGTSNAVNLTDGLDGLAIMPTVMVG
                     SALGIFAYVAGHSVFSKYLGLPYIPGAGELTVFCGALAGAGLGFLWFNAYPAEVFMGD
                     VGALALGAALGTMAVITRQEIVLFIMGGVFVVETLSVMLQVLYFKASGGRRIFRMAPL
                     HHHYELEGWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            770..2149
                     /gene="murD"
                     /locus_tag="VFB53_05005"
     CDS             770..2149
                     /gene="murD"
                     /locus_tag="VFB53_05005"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZN86130.1"
                     /translation="MSMREPPFTLPPRPQPVALAGKRVLVLGLGDSGLSAARWVERQG
                     GRARVADTRAAPPRASDYAGELHTGAFTPALLEDVALVCVSPGLSLAEPLVQQAAARG
                     IPVLGDIELFAWQVRSDPAARVLAITGTNGKTTVTSLTGHLLRTAGIDCAVAGNIGPA
                     ALDALMQRQAPPAAWVLELSSYQLETTWSLEPDAATVLNLSEDHLDRYAGLEAYGAAK
                     ARVFQGAGLQLLNRDDARSMALALPGRELVTFGLDAPSAEGHWGVADGAIHEGALPVL
                     PLHELPIHGAHNVANALAACALARSLGASREALAQGLRGFRGLPHRLQLIAERGGVAW
                     YDDSKGTNVGATVAALRGLGQPAWLIAGGDGKGQDFAPLAPAVRAHARGVLLIGRDAP
                     LIERALAASGVPSLRCATLEDAVARAAQLARPGEAVLLSPACASFDMFRDYRHRGEAY
                     AQAVAALGE"
     gene            2151..3314
                     /gene="ftsW"
                     /locus_tag="VFB53_05010"
     CDS             2151..3314
                     /gene="ftsW"
                     /locus_tag="VFB53_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZN86131.1"
                     /translation="MSATYVFAQEAAAPEYDRSLAWAALLLLAIGTVMVYSASIATAE
                     AARHTGANSAFYLLRHAAFLGVSLAAAIAVFLVPVRFWQGGAPLLFVACVALLVLVLV
                     PGIGREVNGARRWINFGALTLQPSELMKLAAVLYAADYTVRKHAVMKSFRRGLMPMLA
                     VMMFVSWLLLREPDFGALVVIAVTTFGILFVGGMNGRHFLALGAMLALGFALLVLTSE
                     YRMQRIFGFMDPWTDPYGRGYQLSHALIAFGRGEWLGVGLGASVEKLFYLPEAHTDFL
                     LAVIAEELGFLGVAAVIALFVWIVLRAFAIGRQAALRERHFAALAAQGIGIWIGFQAL
                     INMGVNTGLLPTKGLTLPLMSFGGSGLVANCVSLAILLRIDWENRQLARGLHA"
     gene            3329..4363
                     /gene="murG"
                     /locus_tag="VFB53_05015"
     CDS             3329..4363
                     /gene="murG"
                     /locus_tag="VFB53_05015"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZN86132.1"
                     /translation="MAGGTGGHVFPGLAVAEVLRERGWRVVWMGTRAGMEAHLVAARG
                     YELAAIRAAAMRGKGLAAALLLPFNLLAGFWQSARAIFRLRPDVVLGMGGYVAFPGGM
                     MASLLARPLAVHEQNAVAGLTNRILAGIADKAMSAFPQALKGAEWTGNPVRSEIAALA
                     EPAERFARRGGPLKLLVLGGSGGAQALNECVPRALALLEPRPAVVHQSGEKNYAALRA
                     AYAAAGVQGELVPFIDDVARRYAEADLVICRAGAMTVAELSAGGVASVLVPYPHAVDD
                     HQTANARYLADQGAAILLPQTELSPQTLAALLRPLERPRLLEMARKARTLGKPEAARI
                     VAERCMELAA"
     gene            4360..5736
                     /gene="murC"
                     /locus_tag="VFB53_05020"
     CDS             4360..5736
                     /gene="murC"
                     /locus_tag="VFB53_05020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZN86133.1"
                     /translation="MKHKVRHIHFVGIGGSGMSGIAEVLANLGYQVSGSDLAENAATR
                     RLQQIGAKVAHSHAAQNINGADAVVVSSAVRADNPEVQAARARRVPVVPRALMLAELM
                     RLKQGVAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNAAGSNARLGQGEYIVVE
                     ADESDASFLHLQPVVAVVTNIDADHMETYQHDFARLKQAFLQFLQNLPFYGSAIVCVD
                     DANVREILPSVSKPVISYGFAADAMVRAQAAQHAGGAMRFSVLRDKRAPLAVTLNLPG
                     EHNVRNALAAIAVGGEVGVPDAAMLKALAEFRGVGRRFQRCGDVPLAGGGAFALVDDY
                     GHHPAEMAATLAAARGAFPGRRIVLAFQPHRYTRTRDLLGEFARVLANADAVLLAEVY
                     AAGEAPIAGADGRALARAMRAAGGSEPQFVEDIGQMAAAIRGVARDGDVVLTMGAGSI
                     SGVAAQLT"
     gene            5733..6656
                     /gene="murB"
                     /locus_tag="VFB53_05025"
     CDS             5733..6656
                     /gene="murB"
                     /locus_tag="VFB53_05025"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZN86134.1"
                     /translation="MNMADVLRLEGLRGELRAGEPMARHVSWRAGGKAARCYAPADLE
                     DLAQFLRQLPAAEPLLFVGLGSNLLVRDGGYAGTAVLMHAAARHADLIVGRVYAEAGV
                     AAPKVARFAARHALAGAEFLAGVPGTVGGALAMNAGCYGGETWEIVERAVTIDRRGAV
                     RTRGRAEFDIGYRHCALKGGGRLGEDEWFAGAWFDLAKGDAAASRARIRELLEKRIAA
                     QPLSLPNAGSVFRNPPGDHAARLIESCGLKGLARGGARVSEKHANFIVNAKGLGSAAD
                     IEGLIEEVRARVLERTGVALLPEVRIVGERA"
     gene            6653..7555
                     /locus_tag="VFB53_05030"
     CDS             6653..7555
                     /locus_tag="VFB53_05030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZN86135.1"
                     /translation="MKVAVLMGGKSAEREVSLMSGTGVLKALRAKGVDAHAFDPAERD
                     LADLRGEGYARCFIALHGRGGEDGTLQGALEVMGIPYTGSGVLGSAIAMDKWRTKMIW
                     QAAGLPTPRHRILAASDDWSAVARDLGLPLIVKPANEGSTLGLTKVTAAAQLPAAYEL
                     AARKYQDLALAESFVDGPEFTASILGDTVLPLIRIEAPQGNYDYRNKYFGDATKYLCP
                     CGLPASKEAELQALSLKAFQLVGASGWGRVDLMLDAAGAPWLLEVNTAPGMTGHSLVP
                     IAARAVGIPYEDLCVRILEQAHVG"
     gene            7545..8288
                     /locus_tag="VFB53_05035"
     CDS             7545..8288
                     /locus_tag="VFB53_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZN86136.1"
                     /translation="MWDNPRLLNAVAGALTALALLVFAVAALQLLLRSPLFPLREITV
                     SGGLGHTTRAEVEQATQGRVAGNFFAVDLAGVRAALETLPWVRRVQVQRIWPDRIDVR
                     LEEHVPLARWGNAGLVNQQGERFAGRGADAGLPQFAGPQGSEGEVTRRYRRFSEILAP
                     LGAGLERLVLTPRYAWQLRLADGLAIELGRESPNDAVERRLQRFVAVYGQALAKVQRK
                     AASAGAPTMHVDLRYPNGFALRVAEMKGG"
     gene            8292..>9512
                     /gene="ftsA"
                     /locus_tag="VFB53_05040"
     CDS             8292..>9512
                     /gene="ftsA"
                     /locus_tag="VFB53_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZN86137.1"
                     /translation="MAKETKNLVVALDIGTSKVAALVAELRADGSLEILGMGGHESRG
                     LKKGVVVNIEATVNSVQRALEEAELMADCKIVSAFTGIAGSHIKSFNSTGMVAIKDRE
                     VGALDVERAIETAKAVNIPTDQQILHVLRQEFIIDGQEDVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVNDLILQPLASSRAVLSEDEKDLGVCLVDIGGGTSDIA
                     VFTHGAIRHTAVIPIAGDQITNDIAMALRTPTADAETIKVRQGVALRQLADPNEMIEV
                     PGIGDRGPRVMSRQTLAEVIEPRVEELFSLVQQVLRESGFEELLSSGVVLTGGSAVMQ
                     GMVELGEEIFHMPVRIGVPKYAGGLADVVRNPRYATAVGLLLEGVQQIRQGQSSRQDG
                     SVRAVFGRMREWFQR"
BASE COUNT         1268 a         3320 c         3549 g         1375 t
ORIGIN      
        1 gcatcgtcgg ctgggtcgac gactaccgca aagtcgtgca ccgcaatccg aagggcctgt
       61 cggcgaaggc gaagctcttc tggcagtcgc tgatcgggct cgccgcggcg tgctacctcg
      121 gctacctgtc gcagagggtg ccggcgctga acgagctgat cgtgccgttt ttcaagcacg
      181 tcgcctatcc cctcggcatg gtcggcttca tcgtgctcac cttcttcgtc atcgtcggca
      241 cgtcgaacgc ggtcaacctc accgacggcc tcgacggcct cgccatcatg cccacggtga
      301 tggtcggcag cgcgctgggc atcttcgcct acgtggcggg acacagcgtg ttcagcaagt
      361 acctcggcct gccctacatc ccgggcgccg gcgagctgac cgtgttctgc ggtgcgctgg
      421 ccggcgcggg cctggggttc ctctggttca acgcctaccc ggccgaagtg ttcatgggcg
      481 acgtcggcgc gctggcgctg ggcgccgcgc tcggcaccat ggcggtgatc acgcggcagg
      541 agatcgtgct gttcatcatg ggcggcgtgt tcgtggtcga gacgctgtcg gtgatgctgc
      601 aggtgctgta cttcaaggcc tcgggcggcc ggcgcatctt ccgcatggcg ccgctgcacc
      661 accactacga gctggaaggc tggaaggaat cccaggtggt ggtgcgcttc tggatcatca
      721 ccatgatgct ggtgctgttc gggctgtcga cgctgaagct gcggtgacca tgtcgatgcg
      781 cgagccgccc ttcacccttc cgccgcgccc gcagccggtg gcgctcgccg gcaagcgcgt
      841 gctggtgctc gggctgggcg acagcggtct gtcggcggcc cgctgggtgg agcgccaggg
      901 cggccgcgcg cgcgtcgccg acacgcgcgc cgcgccgccg cgcgcctccg actacgcggg
      961 cgagctgcac accggcgcgt tcacgccggc gctgctcgag gacgtcgcgc tggtgtgcgt
     1021 cagcccgggc ctgtcgcttg cggagccgct cgtgcagcag gcggcggcgc gcggcatccc
     1081 ggtgctcggc gacatcgagc tgttcgcctg gcaggtgcgc agcgaccccg ccgcgcgggt
     1141 gctggcgatc accggcacca acggcaagac caccgtcacg tcgctcaccg ggcacctgct
     1201 gcgcaccgca ggcatcgact gcgcggtggc gggcaacatc gggccggccg cgctggatgc
     1261 gctgatgcag cgccaggcgc cgccggcggc ctgggtgctg gaactctcca gctaccagct
     1321 ggagaccacc tggtcgctcg agccggacgc cgccacggtg ctcaacctga gcgaagacca
     1381 cctggaccgc tacgccggcc tggaagcata cggcgccgcc aaggcgcgcg tgttccaggg
     1441 cgcaggcttg cagctgctga accgcgacga cgcgcgctcg atggcgctcg cgctgccggg
     1501 ccgggaactg gtcaccttcg ggctcgacgc gccaagcgcc gaagggcact ggggcgtggc
     1561 ggacggcgcg atccatgagg gcgcgctgcc cgtgcttccg ctgcacgagc tgccgatcca
     1621 cggcgcgcat aacgtcgcca acgcgctggc cgcctgcgca ctggcgcgct cgctcggggc
     1681 ctcgcgcgaa gccctggcgc aggggctgcg cggcttcagg ggcctgccgc accggctgca
     1741 gctgatcgcc gagcgcggcg gcgtcgcctg gtacgacgat tcgaagggca ccaacgtcgg
     1801 cgccaccgtg gccgcgctgc gcggcctcgg ccagcccgcc tggctgatcg ccggcggcga
     1861 cggcaaggga caggacttcg cgccgctcgc cccggcagtg cgcgcgcatg cgcgcggcgt
     1921 gctgctgatc ggccgcgacg cgccgctcat cgagcgcgcg ctcgccgcga gcggggtgcc
     1981 gtcgctgcgc tgcgcgacct tggaagacgc ggtggcgcgc gccgcgcagc tggcgcggcc
     2041 cggcgaggcc gtgctgctct cgccggcctg cgccagcttc gacatgttcc gcgactaccg
     2101 ccaccgcggc gaggcctacg cgcaggccgt ggctgcgctc ggggaatgac atgagcgcca
     2161 cctacgtctt cgcgcaggaa gccgccgcgc ccgagtacga ccgctcgctc gcctgggcgg
     2221 cgctgctgct gctggcgatc ggcacggtga tggtgtactc ggcctccatc gccactgccg
     2281 aggcggcgcg gcataccggc gccaattcgg ccttctacct gctgcgccac gccgccttcc
     2341 tcggcgtgtc gctcgccgcg gcgatcgcgg tgttcctggt cccggtgcgc ttctggcagg
     2401 gcggcgcgcc gctcctgttc gtcgcctgcg tggcgctgct ggtgctggtg ctggtgccgg
     2461 gcatcggccg cgaggtgaac ggcgcgcggc gctggatcaa tttcggcgcc ctgacgctgc
     2521 agccctcgga gctgatgaag ctcgccgccg tgctgtacgc cgccgactac acggtgcgca
     2581 agcacgcggt gatgaagagc ttccgccgcg ggctgatgcc gatgctggcg gtgatgatgt
     2641 tcgtgtcctg gctgctgctg cgcgagccgg atttcggcgc gctggtggtg atcgcggtca
     2701 ccaccttcgg catcctgttc gtcggcggca tgaacggacg gcacttcctc gcgctcggcg
     2761 cgatgctggc gctcggcttc gcgctgctgg tgctgacctc cgagtaccgc atgcagcgca
     2821 tcttcggctt catggatccg tggaccgatc cctacggccg cggctaccag ctgtcgcacg
     2881 cgctgatcgc cttcggccgc ggcgaatggc tgggcgtcgg cctgggcgcc agcgtggaga
     2941 agctgttcta cctgccggag gcgcacaccg acttcctgct cgcggtgatc gccgaggagc
     3001 tcggctttct cggcgtggcg gcggtgatcg cgctgttcgt gtggatcgtg ctgcgcgcct
     3061 tcgccatcgg ccgccaggcg gcgctgcgcg agcgccactt cgccgcgctc gccgcgcagg
     3121 gcatcggcat ctggatcggc ttccaggcgc tgatcaacat gggcgtgaac acgggcctgc
     3181 tgcccaccaa gggcctgacc ctgccgctga tgagcttcgg cggcagcggc ctggtggcca
     3241 actgcgtctc gctggcgatc ctgctgcgca tcgactggga aaaccgccag ctggcgcggg
     3301 ggctgcacgc gtgaggaccg cgctgatcat ggccggcggc accggcggcc acgtgttccc
     3361 ggggctggcg gtggccgagg tgctgcgcga gcggggctgg cgcgtggtgt ggatgggcac
     3421 gcgcgcgggg atggaggcgc acctggtcgc cgcgcgcggc tacgaactgg cggcgatccg
     3481 cgccgcggcg atgcgcggca agggactcgc cgcggcgctg ctgctgccgt tcaacctgct
     3541 cgccggcttc tggcagtcgg cgcgggcgat cttccgcctg cgcccggacg tggtgctcgg
     3601 catgggcggg tacgtggcgt ttcccggcgg catgatggct tcgctcctgg cgcggccgct
     3661 cgccgtgcac gagcagaacg ccgtcgccgg gctcaccaac cgcatcctgg ccggcatcgc
     3721 ggacaaggcg atgagcgcgt tcccgcaggc gctgaaaggc gccgaatgga ccggcaaccc
     3781 ggtgcgcagc gagatcgccg cgctggccga gcccgccgag cgcttcgcgc ggcgcggcgg
     3841 cccgctgaag ctcctggtgc tgggcggctc gggcggcgcg caggccttga acgagtgcgt
     3901 gccgcgggcg ctcgcgctgc tcgagccgcg gcccgcggtg gtgcaccagt cgggcgagaa
     3961 gaactacgcc gcgctgcgcg cggcctacgc ggcggccggc gtgcaggggg aactcgtccc
     4021 cttcatcgac gacgtggcgc ggcgttacgc ggaagccgac ctggtgatct gccgcgccgg
     4081 cgcgatgacg gtggcggagc tctcggccgg cggggtggcg agcgtgctgg tgccgtatcc
     4141 gcacgcggtg gacgaccacc agaccgccaa tgcgcgctat ctcgccgacc agggcgccgc
     4201 catcctgctg ccgcagaccg agctgtcgcc gcagacgctc gccgcgctgc tgcgcccgct
     4261 cgagcggccg cggctgctcg agatggcgcg caaggcgcgc accctgggca aaccggaggc
     4321 ggcgcgcatc gtcgccgagc gctgcatgga gctcgccgcg tgaagcacaa ggtgcgccac
     4381 atccatttcg tcggcatcgg cggctcgggc atgtccggca tcgccgaagt cctcgccaac
     4441 ctcggctacc aggtgagcgg ctcggacctc gccgagaacg ccgccacgcg gcgcctgcag
     4501 cagatcggcg ccaaggttgc gcactcgcat gccgcgcaga acatcaacgg cgccgacgcg
     4561 gtcgtggtgt cgagcgcggt gcgcgcggac aaccccgagg tgcaggcggc gcgcgccagg
     4621 cgcgtgccgg tggtgccgcg cgctctgatg ctcgccgagc tgatgcggct gaagcagggc
     4681 gtggcgatcg ccggcacgca cggcaagacg acgaccacca gcctggtggc gagcgtgctg
     4741 gccgagggcg gcctggatcc caccttcgtc atcggcgggc ggctgaacgc cgccggctcc
     4801 aacgcgcgcc tgggccaggg cgaatacatc gtggtcgagg ccgacgaatc cgacgcctcg
     4861 ttcctgcacc tgcaaccggt ggtggcggtg gtgaccaaca tcgacgccga ccacatggag
     4921 acctaccagc acgacttcgc gcgcctgaag caggcgttcc tgcagttcct gcagaacctg
     4981 cccttctacg gcagcgccat cgtgtgcgtg gacgacgcca atgtgcgcga gatcctgccg
     5041 tcggtgtcca agccggtgat cagctacgga tttgccgccg acgccatggt gcgcgcgcag
     5101 gctgcgcagc atgccggggg cgcgatgcgc ttcagcgtgc tgcgcgacaa gcgagcgccg
     5161 ctcgcggtga ccctcaacct gccgggggag cacaacgtgc gcaacgcgct cgcggcgatc
     5221 gcggtgggcg gcgaagtcgg cgtgccggat gcggcgatgc tcaaggcgct ggcggaattc
     5281 cgcggcgtcg ggcggcgctt ccagcgctgc ggcgacgtgc cgctcgccgg cggcggcgcc
     5341 ttcgcgctgg tcgacgacta cggccaccac ccggcggaga tggcggcgac gctcgccgcc
     5401 gcgcgcggcg cctttcccgg gcggcgcatc gtgctcgctt tccagccgca ccgctacacg
     5461 cgtacgcgcg acctgcttgg cgaattcgcc cgggtgctgg cgaacgccga cgcggtgctg
     5521 ctcgccgagg tgtacgccgc cggcgaggcg ccgatcgccg gcgccgacgg ccgcgcgctg
     5581 gcgcgcgcga tgcgcgccgc cggcgggtcc gaaccgcagt tcgtcgagga catcgggcag
     5641 atggccgcgg cgatccgcgg cgtggcgcgc gacggcgacg tggtgctgac catgggcgcg
     5701 ggctcgatca gcggcgtggc cgcgcaactg acatgaacat ggcggacgtg cttcgactgg
     5761 agggactgcg cggcgaactg agggccggcg agccgatggc gcgccacgtg agctggcgcg
     5821 cgggcggcaa ggcggcgcgt tgctatgcgc cggcggacct cgaggacctg gcgcagttcc
     5881 tgcgccagct gccggcggcc gaaccgctgc tgttcgtcgg gctgggctcc aacctgctgg
     5941 tgcgcgacgg cggctacgcc ggcacggcgg tgctgatgca cgccgcggcg cgccatgccg
     6001 atctgatcgt cggccgcgtg tacgccgagg ccggcgtggc ggcccccaag gtggcgcgct
     6061 tcgccgcgcg ccatgcgctc gccggcgccg agttcctcgc cggcgtgccg ggaacggtcg
     6121 gcggcgcgct ggccatgaac gcgggctgct acggcggcga gacctgggag atcgtcgagc
     6181 gcgccgtcac catcgaccgg cgcggcgcgg tgcgcacgcg cggcagggcc gagttcgaca
     6241 tcggctaccg gcattgcgct ctcaagggcg gcggccgcct cggcgaggac gagtggttcg
     6301 ccggcgcctg gttcgacctg gccaagggcg atgccgcggc atcgcgcgcg cgcatccgcg
     6361 agctgctcga gaagcgcatc gccgcgcagc cgctttccct gcccaatgcg ggcagcgtgt
     6421 tccgcaaccc gcccggcgac cacgccgcgc gcctcatcga gtcctgcggc ctgaagggcc
     6481 tggcgcgcgg cggcgcgcgg gtctcggaga agcacgccaa cttcatcgtc aacgccaagg
     6541 gcctgggcag cgccgccgac atcgaggggc tgatcgagga ggtgcgcgcg cgcgtgctcg
     6601 agcgcaccgg ggtcgcgctg ctgcccgaag tgcgcatcgt cggggagcgc gcatgaaagt
     6661 cgcggtcctc atgggcggca agtccgcgga gcgcgaagtg tccctgatga gcggcacggg
     6721 ggtgttgaag gcgctgcgcg ccaagggcgt cgacgcccat gccttcgatc ccgcggaacg
     6781 cgacctcgcg gacctgcgcg gcgagggcta tgcgcgctgc ttcatcgccc tgcacggccg
     6841 cggcggcgag gacggcacgc tgcagggcgc gctcgaggtc atgggaattc cctacacggg
     6901 gagcggggtc ctgggctcgg cgatcgccat ggacaaatgg cgcacgaaga tgatctggca
     6961 ggccgccggc ctgccgacgc cgcgccaccg catcctggcc gcgagcgacg actggagcgc
     7021 tgtggcgcgc gacctcggcc tgccgctgat cgtcaagccg gcgaacgaag gctcgacgct
     7081 cggcctgacc aaggtgacgg ctgccgcgca gctgcccgcg gcctacgagc tcgccgcgcg
     7141 caagtaccaa gaccttgcgc tggcggagag cttcgtcgac gggccggaat tcaccgccag
     7201 cattctcggc gacacggtgc tgcccctgat ccggatcgag gcgccgcagg gcaactacga
     7261 ctaccggaac aagtacttcg gcgacgccac gaagtacctg tgcccctgcg gcctgccggc
     7321 gtccaaggaa gcggaactgc aggccctgtc gctcaaggcc ttccaactgg tgggcgccag
     7381 cggctggggg cgcgtcgacc tgatgctcga cgcggcgggc gcgccatggc tgctcgaggt
     7441 caacaccgcg cccggcatga ccggccacag cctggtgccg atcgcggcgc gcgccgtcgg
     7501 catcccgtac gaggacttgt gcgtgcgcat cctggagcag gcccatgtgg gataacccgc
     7561 gcctgctcaa cgcggtggcc ggcgcgctca ccgcgctcgc cctgctggtg ttcgcggtcg
     7621 cggcgctgca gctgctgctg cgctcgccgc tgtttccgtt gcgcgagatc acggtgagcg
     7681 gcggcctggg acacaccacg cgcgccgagg tggaacaggc cacgcagggg cgcgtggccg
     7741 gcaacttctt cgccgtcgac ctggcaggcg tgcgcgcggc gctcgaaacc ctgccctggg
     7801 tgcggcgcgt gcaggtgcag cgcatctggc cggaccgcat cgacgtcagg ctcgaggagc
     7861 acgtcccgct ggcgcgctgg ggcaacgccg gcctggtcaa tcagcaaggc gagcgcttcg
     7921 ccggacgcgg cgccgacgcc ggcctgccgc agttcgccgg accgcagggc agcgaaggcg
     7981 aggtgacgcg ccgctaccgc cgcttcagcg agatcctggc gccgctcggc gccggcctgg
     8041 agcggctggt gctgacgccg cgctacgcct ggcagctgcg ccttgccgac gggctggcga
     8101 tcgagctggg ccgcgagtcc cccaacgacg cggtcgagcg gcgcctgcag cgcttcgtcg
     8161 cggtgtacgg gcaggcgctg gcgaaggtgc agcgcaaggc ggcgagcgcc ggcgcgccga
     8221 ccatgcacgt cgatctgcgc tatcccaacg gcttcgcgct gcgcgtggcc gaaatgaagg
     8281 gcggttgagc gatggccaag gaaaccaaga acctcgtcgt ggcgctggac atcggcacct
     8341 ccaaggtggc ggcgctggtg gccgagctgc gcgccgacgg atccctggag atcctcggca
     8401 tgggcggcca cgagtcgcgc gggctgaaga agggcgtggt ggtgaacatc gaggccaccg
     8461 tcaactccgt gcagcgcgcg ctcgaggagg ccgagctgat ggccgactgc aagatcgtct
     8521 cggcgttcac cggcatcgcc ggcagccaca tcaagagctt caactccacc ggcatggtgg
     8581 cgatcaagga ccgcgaggtg ggcgcgctgg acgtcgagcg cgccatcgag acggcgaagg
     8641 cggtgaacat ccccaccgac cagcagatcc tgcacgtgct gcggcaggaa ttcatcatcg
     8701 acggccagga ggacgtgcgc gagccgatcg gcatgtccgg cgtgcgcctg gaggtgaagg
     8761 tgcacatcgt caccggcgcg gtgtcggcgg cgcagaacat cgtgaagtgc gtgcgccgct
     8821 gcgggctgga ggtcaacgac ctgatcctgc aaccgctcgc ctcctcgcgc gccgtgctgt
     8881 cggaggacga gaaagacctc ggcgtgtgcc tggtggacat cggcggcggc accagcgaca
     8941 tcgcggtgtt cacgcacggc gcgatccgcc ataccgcggt gatcccgatc gccggcgacc
     9001 agatcaccaa cgacatcgcc atggccctgc gcacgccgac cgcggacgcc gagaccatca
     9061 aggtgcgcca gggcgtggcg ctgcgccagc tggccgaccc caacgaaatg atcgaggtgc
     9121 cgggcatcgg cgaccgcggc ccgcgcgtca tgtcgcgcca gacgctcgcc gaggtgatcg
     9181 agccgcgcgt cgaggaactg ttctcgctgg tgcagcaggt gctgcgcgag tccggcttcg
     9241 aggagctgct gtcctcgggg gtggtgctca ccggcggcag cgcggtgatg cagggcatgg
     9301 tggagctggg cgaggagatc ttccacatgc cggtgcgcat cggcgtgccg aagtacgcgg
     9361 gcggcctggc ggacgtggtg cgcaatccgc gctacgccac cgccgtgggg ctgctgctcg
     9421 agggcgtgca gcagatcagg caggggcagt cttcaaggca ggacggttcg gtgcgcgcgg
     9481 tgttcggccg catgcgcgag tggttccaga gg
//
LOCUS       DASRIE010000114        12171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3241439, whole genome shotgun sequence.
ACCESSION   DASRIE010000114 DASRIE010000000
VERSION     DASRIE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241439"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="hrcA"
                     /locus_tag="VFB53_05045"
     CDS             <1..818
                     /gene="hrcA"
                     /locus_tag="VFB53_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZN86138.1"
                     /translation="LGYRFFVDTLLVVKQLESGELLQLEDQLHPDNPQRAIRAASQLL
                     SQLTQFAGVVMTPRRRALSFRQIEFLRLSEKRVLLIVVTQDGDVQNRVLFTERDYSPA
                     ELVSAANYINQNYAGHSFEEVRQRLHRELHEIRQDMMRLMSAALEAGNQAVTEGSEPY
                     VISGERNLLAAQDLSQDMGRMRQLFELFEHKTTLLQILDLSLRGQGVQIFIGGESGVN
                     APDEVSVVTAPYKVDGEVVGTVGVIGPSRMAYDRVVPIVDITAKLLSSALSRP"
     gene            824..1705
                     /gene="hemH"
                     /locus_tag="VFB53_05050"
     CDS             824..1705
                     /gene="hemH"
                     /locus_tag="VFB53_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZN86139.1"
                     /translation="MLLVNLGTPAAPTPAAVRSYLAEFLSDPRVVELPRWLWLPILHG
                     FVLRRRPAQSAAKYAKIWTPEGSPLAVHTARQALLLRAATGLPVEYAMRYGEPGIAAA
                     LQRLPGKVRVLPLYPQFARSTTASVRDVVGDGVDFVEQFHDHPAYIAALAALVEARPD
                     VLVMSFHGLPQRAVERGDPYRDQCLRTAQLLAAALGLREDQYRVSFQSRFGPARWLGP
                     ATAQVLAELGAARTPRVHVICPGFVADCLETLEEIALEGRTVFLDAGGGEFRALPCLN
                     ESPLWIEALRRIATASA"
     gene            complement(1689..1898)
                     /locus_tag="VFB53_05055"
     CDS             complement(1689..1898)
                     /locus_tag="VFB53_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86140.1"
                     /translation="MRCLLILLLAALPARAEDNAVERGLKKAGKAAEHGIKSAGRAVD
                     RGAKAVQKGAEKVHRKIDEKVRPKQ"
     gene            complement(1911..3185)
                     /locus_tag="VFB53_05060"
     CDS             complement(1911..3185)
                     /locus_tag="VFB53_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN86141.1"
                     /translation="MQLGKYPILREIGRGATSHVYLARDPFADREVAIKVFLFDQHAD
                     PQTERMMHKAFLAEASLAGKLNHPHIVDILDAVAEPDHSYLVMEYVAGTALDAHADVS
                     TLLPLDKVVEIIFKCIRALEYAFQHGVIHRDIKPGNILLSAAGETKVGDFGASFQQRL
                     GQETTQMTGVGSPAYMSPEQIRMEPLTAQTDIYSLGVTMYRLLTGRLPYQASTQAALT
                     YAILNTPPVRPAKLRPDLPPLLDQIVMSAINKDPAQRYRSWLEFGKDLSQAFATLRLV
                     GESLSDSAKFNELRQLAFFEGFGDVALWEVVRIGSWKKIKAGTAIIREGEHGDSFYLL
                     IAGEVSVTLSGKKLNTIKPGGCFGEILYFADRIERRTTTITALSDITVIEIKAEAMRA
                     ATDACQVGLNKAFMRMLIERLMQANVQLAARG"
     gene            complement(3214..8283)
                     /locus_tag="VFB53_05065"
     CDS             complement(3214..8283)
                     /locus_tag="VFB53_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZN86142.1"
                     /translation="MSNASATILSWITAEVDQALRQVREHIAKFSADPGDAALLGPCP
                     GHLHQVSGALRMVGLAGATRFCEAIEGGFAGLNGARPSSATIGVIDRAVLALKDFVDG
                     LERGQANVPLRLFPVYRELSTLPGGKGASEKDLFYPDLTLQAPAHPEPRSLPAAEVAP
                     YLQAQRTQFQRGLLGALRNQSGGLQEMRQALDALHRVAAQLPEPRALWWAAAGLLEGF
                     AAPPDADWLARAKALCNKIDFQVRDLAAGAPAVSEALLREVLYAVAKCKPATPRVKEV
                     KQLYLLDGLFPEAQGAPGTVEFDMDWLQPALSDVRSRLEALKSLWLQYVSGEPKSAAR
                     FRELVAAFRAKAGALGNPHLVKLLDAIALVTTRLPDPFPPQSQLLVIEMASAFLLAES
                     VVENFTDPAADLEQQIGIMGGWLLDAANGKSTGEPPAGLRADLSERIGALQLRAQVAR
                     EIAANLQRVEQILDGFARDPGKRDTLPGLAPDLRQIHGALNVLGFARAAQILPLCEKL
                     IEACAQAEHDPAGDAMDWIAEGLSSVGLFLDPCLNGREPAEQAVELFFRRYDKAHAPA
                     ETLSHDSTVVLTAAALKGAAAPAPVAAAVDAGAIAEAAAASLAAAPTSFAPQSGPAAS
                     RQQVNEELLAIYLEEAGEVLANIDAALPAARAQPQDRDALTTIRRGFHTLKGSGRMVG
                     LMDLGEVAWEVEQVMNKWLEQQRPATPGLLELIEGASAAFADWVAQLKEGRLQAEITG
                     AGIVELARRLKTEEAPAAAPVAVPAPAPAAAPAPEPLAELLPEPEPQLAEPPPVPEEV
                     EVAGLRLARSLFDIYLAEAGQHTASLEAQCAAWRRKQGAEASHEFVRAAHTLASSSRT
                     AGFVEVATLAEALEQWMPLARLTGAADAGTVQAAILALRTMADALARREAPTPAPQVL
                     RALQDLAARLRAPGKPRPQAEAPKPAPEAGGRNKRVLHDDIDAQLLPIFLEEAQQLLP
                     LIGGDLRDWKANPADPQVLQSLQRALHTLKGSARMAGAIRLGELTHLMESRIEAAVEA
                     GAIAPELFVELEEKMDRLSLDLERMAAGPEPEPAAAEAPRAEAAARAEPPLPSPAAML
                     RINADTLDHLINESGEVSIARSRVEGELRAVKQSIADLSDSIARMRGQLREVEIQADS
                     QMQSRLSVLEEQRSEFDPLEFDRYTRLQELTRLMAESLHDAASIQQALHKNLGETDAA
                     LTHQARISRDVQQELMRMRAVPFSILDERLYRIVRQSTRELDKKAELEIQGSQVEVDR
                     SVLDRIGAPLEHMLRNALVHGIESPQARAAAGKPETGRIAISLRQESNEIALVVSDDG
                     AGLDLASLRRKAGEMGLLAPGREPTEAELTQLIFVSGLSTAGEVTELAGRGIGMDVVK
                     NEITAIGGRIEVATSAGQGTVLTLYLPLTLAVTQAVLVRAGGGILAVASAMVEQVLRL
                     KSDAMAARFADGSVEFQERSYPLYSLAQLLGAAGPGEAQSYHSVLLLRSGIQRIALQV
                     DELIGNQEIVVKNIGPQLARVPGVAGATVLADGSIVLIVNPVQLAQHARAAPERATST
                     LPAPAAAAAPVVMVVDDSLTVRKITSRLLEREGYRVLTAKDGLDAIEQMKDALPAVML
                     VDIEMPRMDGFDLARHVRGDPGTRHVPIIIISSRTAEKHRSQAAQLGVNVFLGKPYPE
                     AELLQHIARYVAAGQDVAVAL"
     gene            complement(8290..10404)
                     /locus_tag="VFB53_05070"
     CDS             complement(8290..10404)
                     /locus_tag="VFB53_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZN86143.1"
                     /translation="MALNLGSLKLPFAGRGAEPAPAPAAAAAQGGGLWLIGRLAIGRQ
                     LQVLIGALVLVLGAIAAVVVYDTRQGTFATIYIATAGELQMLSQRLAKAAQQASQGNT
                     EAFKQLRASRDEFAALVKLLAFGGASGGVSLPPTSAGVQPLLNALDREWGKTERNAAL
                     VIDEERNLVALGRAVRAINTNNPVLQELTDEIAALSVQGGGSARQNAIAAQLTMLTQR
                     MAKNANTMLAENIVDPEVAFLLGKDTNTYRDTLQGLLQGSEAMRIARVQDAELRGKLG
                     ELQAAFADYQRAVSDILGNQARLVGAKRAAFQMFNDSESLLQSAERLGQAYEAELGGR
                     QFNYLIIGAMGVLALLVLLLVGKVYIDDSRRRAASSEQLNRTNQEAILRLLDEIGNLA
                     DGDLTAKAHVTEEITGAIADSINYTIDELRRLVSGITDAAQQVTSATAEAQAVSGQLL
                     QAAQRQATEIQGTGQSVVQMAQSMTGVSKSAGDSARVARTSLNAAAKGTQAVQNAIRG
                     MNDIREQIQETSKRIKRLGESSQEIGEIVQLISDITEQTNVLALNAAIQAASAGEAGR
                     GFTVVAEEVQRLAERSGEATKHIGAIVKSIQRDTQDAVEAMERSTRGVVDGTKTADEA
                     GGALREIEQVSNQLAELIGSISDATEQQSISATRVAAAMNEILAITQMTTDGTRRTAA
                     SAERLTALAEGLKASVSGFKLA"
     gene            complement(10416..11555)
                     /locus_tag="VFB53_05075"
     CDS             complement(10416..11555)
                     /locus_tag="VFB53_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN86144.1"
                     /translation="MKRNTIRRVIAAAAAMLAQGAAAQQVGVTADKILLGQAAVFSGP
                     AAQLGIQMRNGIKSYLDHVNATGGVHGRRIELITEDDRYEVAVAPGASKKLIEEHKVF
                     ALLGYVGTPTGVVHLPVVTQAKVPLVGMFTGAEALRVPFNRYVFHVRASYYDETDKIV
                     EQVVSIGGRNIAVFYQNDAYGQAGLKGVEIATQKRNMKISALGTVERNTIKVEEAVKS
                     IHAAKPDAVVMISAYTSCAEFIRQMQKAGSAATFYNVSFVGSQALADALGKDGVGVAI
                     SQVVPFPWGRAVPVVKEYQQLAAKAGFKDYNFSAMEGFLSAKVSVEGLRRAGRNLTRE
                     GYIAALEKMQSVDLGGFFVSYSPTNHAGSKFVDLTIIGRGGKFLR"
     gene            complement(11581..12090)
                     /locus_tag="VFB53_05080"
     CDS             complement(11581..12090)
                     /locus_tag="VFB53_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZN86145.1"
                     /translation="MAQRISLRDYQRELAERLRAADSARNASKLGLQVGAQRWLIDLA
                     EAGEVIPVPPITPVPLTQPWFRGVANIRGNLCSVVDFAAFLGASPAPPGEQARLVLFG
                     ARFRTGGALLVDRSLGLRNAEELQPQSASGPQAAWLKGEYSDAQGERWKELDVAQLVA
                     DPGFLAVGA"
BASE COUNT         1598 a         4454 c         4164 g         1955 t
ORIGIN      
        1 cgctcggcta ccgcttcttc gtcgacacgc tgctggtggt caagcagctc gagtcgggcg
       61 agctgctcca gctcgaggac cagctgcacc cggacaaccc gcagcgcgcg atccgcgccg
      121 cctcgcagct gctgtcgcag ctcacccagt tcgccggcgt cgtgatgacc cctcgccggc
      181 gcgcgctgtc gttccgccag atcgagttcc tgcgcctgtc ggagaagcgc gtgctgctca
      241 tcgtggtgac gcaggacggc gacgtgcaga accgggtgct gttcaccgag cgcgactact
      301 cgcccgccga gctggtgtcg gcggccaact acatcaacca gaactacgcc ggccacagct
      361 tcgaggaggt gcgccagcgc ctgcaccggg agctgcacga gatccgccag gacatgatgc
      421 ggctgatgag cgcggcgctc gaggccggca accaggcggt gaccgagggc agcgagccct
      481 acgtcatctc tggcgagcgc aacctgctcg cggcgcagga cctgtcgcag gacatgggac
      541 gcatgcgcca gctgttcgag ctgttcgagc acaagaccac gctgctgcag atcctcgacc
      601 tgtcgctgcg cggccagggc gtgcagatct tcatcggcgg ggagtccggc gtgaatgcgc
      661 ccgacgaggt cagcgtggtg accgcgccct acaaggtgga cggcgaggtg gtcggcacgg
      721 tgggcgtgat cggcccgtcg cgcatggcct acgaccgcgt ggtgccgatc gtcgacatca
      781 ccgccaaact gctctccagc gccctgtcca ggccctgaac ggcatcctcc tcgtcaacct
      841 cggcacgccg gcggcgccca cgccggcggc ggtgcgcagc tatctcgccg agttcctgtc
      901 cgatccgcgc gtggtcgagt tgccgcgctg gctgtggctg ccgatcctgc acggcttcgt
      961 gctgcgccgc cgcccggcgc agtcggcagc caagtacgcc aagatctgga cgccggaagg
     1021 ctcgccgctc gcagtgcaca ccgccaggca ggcactgctg ctgcgcgcag ccaccggctt
     1081 gccggtcgag tacgccatgc gctacggcga acccggcatc gccgccgccc tgcaaaggct
     1141 gcccgggaag gtccgcgtcc tcccgctgta tccccaattc gcgcgcagca ccactgcatc
     1201 ggtgcgcgac gtcgtcggcg acggcgtcga tttcgtcgag cagttccacg accaccccgc
     1261 ctacatcgcg gcgctcgccg cgctggtcga ggcgcgcccc gatgtgctgg tgatgagctt
     1321 ccacggcctg ccgcagcgcg ccgtggagcg cggcgacccc tatcgcgacc agtgcctgcg
     1381 caccgcgcag ctgctggcgg cggcgctggg cttgcgcgag gaccagtacc gggtgtcgtt
     1441 ccagtcgcgc ttcggaccgg cgcgctggct cgggcccgcc accgcgcagg tgctggccga
     1501 gcttggcgcg gcccgcacgc cccgcgtgca cgtgatctgc cccggcttcg tggccgactg
     1561 cctggagacg ctggaggaga tcgccctcga gggccggacg gtgttcctcg acgcgggcgg
     1621 cggcgagttc cgcgccctgc cctgcctcaa tgaatcgccg ctgtggatcg aggcgctgcg
     1681 ccggatcgct actgcttcgg cctgaccttc tcgtcgatct tgcggtgcac cttctcggcg
     1741 cccttttgca ccgccttggc gccgcggtcg accgcgcggc ccgcgctctt gatgccgtgc
     1801 tcggccgcct tgccggcctt cttcaggccg cgctccaccg cgttgtcctc ggcccgcgcc
     1861 ggcagcgcgg ccagcagcag gatcaacagg catcgcatca tcgggacctc ctagccgcgc
     1921 gcggcaagtt gtacgttggc ctgcatcagg cgctcgatca gcatgcgcat gaacgccttg
     1981 ttgaggccga cctggcaggc gtcggtggcg gcgcgcatcg cctccgcctt gatctcgatc
     2041 accgtgatgt cgctgagcgc ggtgatggtg gtggtgcgcc gttcgatgcg gtcggcgaag
     2101 tacaggattt cgccgaagca gccgcccggc ttgatggtgt tcagtttctt gcccgacagg
     2161 gtgacgctga cctcgccagc gatcagcaga tagaagctgt cgccgtgctc gccctcgcgg
     2221 atgatcgccg tgccggcctt gatcttcttc cagctgccga tgcgcaccac ctcccacagc
     2281 gccacgtcgc cgaaaccctc gaagaacgcc agctggcgca gctcgttgaa cttggccgaa
     2341 tcggacagcg attcgccgac cagccgcagg gtggcgaagg cctggctcag gtccttgccg
     2401 aactcgagcc aggagcggta gcgctgcgcc ggatccttgt tgatcgcgct catcacgatc
     2461 tggtcgagca gcggcggcag gtcgggccgc agcttcgccg ggcgcacggg cggcgtgttg
     2521 aggatggcgt aggtcagcgc ggcctgggtc gaggcctggt agggcaggcg cccggtcagc
     2581 aggcggtaca tggtcacgcc cagggagtag atgtcggtct gcgcggtgag cggctccatg
     2641 cggatctgct cgggcgacat gtaggcgggc gagcccacgc cggtcatctg cgtggtctcc
     2701 tgtcccagcc gctgctggaa cgaggcgccg aagtcgccga ccttggtctc ccccgccgcc
     2761 gagagcagga tgttccccgg cttgatgtcg cggtggatga cgccgtgctg gaaggcgtac
     2821 tccagcgcac ggatgcactt gaagatgatc tccaccacct tgtcgagcgg cagcagggtg
     2881 ctgacgtcgg cgtgcgcgtc cagcgccgtg ccggcgacgt attccatcac caggtagctg
     2941 tggtcgggct cggccaccgc gtcgaggatg tcgacgatgt gcgggtggtt gagcttgccg
     3001 gcgagcgacg cctcagcgag gaaggccttg tgcatcatgc gctcggtctg cgggtcggcg
     3061 tgctggtcga acaggaacac cttgatcgcc acctcgcggt cggcgaacgg gtcgcgcgcc
     3121 aggtacacgt ggctggtggc gccgcgtccg atctcgcgca ggatcgggta cttgccgagc
     3181 tgcacctgcg gctcggcgcc gctgccgcgg gcgctagagg gcgacggcga catcctgtcc
     3241 ggcggcgacg tagcgggcga tgtgctgcag cagctcggct tccgggtagg gcttgcccag
     3301 gaacacgttg acgcccagct gcgccgcctg gctgcggtgc ttttcggcgg tgcgcgagga
     3361 aatgatgatg atcggcacgt ggcgcgtgcc cgggtcgccg cgcacgtggc gcgccaggtc
     3421 gaagccgtcc atgcgcggca tctcgatgtc caccagcatc accgcgggca gagcgtcctt
     3481 catctgctcg atcgcatcca ggccgtcctt ggcggtgagc acccggtagc cctcgcgctc
     3541 cagcagccgg ctggtgatct tgcgcacggt gagcgaatcg tccaccacca tcaccaccgg
     3601 cgccgctgcg gcggccggcg ccggcagcgt gctggtggcg cgctccggcg cggcgcgcgc
     3661 atgctgcgcc agctgcaccg ggttcacgat cagcacgatg ctgccgtcgg cgagcaccgt
     3721 ggcacccgcc acgccgggca cgcgcgcgag ctgcgggccg atgttcttca ccacgatctc
     3781 ctggttgccg atcagctcgt cgacctgcag cgcgatgcgc tggatgccgc tgcgcagcag
     3841 cagcaccgaa tggtagctct gcgcttcgcc gggaccggcg gcgcccagca gctgcgccag
     3901 gctgtacagc gggtagctgc gctcctggaa ctcgacgctg ccgtcggcga agcgcgccgc
     3961 catggcgtcg gacttcaggc gcagcacctg ctccaccatc gccgaggcca ccgccaggat
     4021 gccgcccccg gcgcgcacca gcaccgcctg cgtcaccgcc agcgtgagcg gcaggtagag
     4081 agtgagcacc gtgccctggc cggcggaggt ggccacctcg atgcgcccgc cgatggcggt
     4141 gatctcgttc ttcaccacgt ccatgccgat gccgcggccg gcgagctcgg tgacctcgcc
     4201 ggcggtagac aggccggaga cgaagatcag ctgcgtcagt tccgcctcgg tgggctcccg
     4261 gccgggcgcc agcaacccca tctccccggc cttccggcgc aagctcgcca ggtcgaggcc
     4321 ggcgccgtcg tcgctcacca ccagggcgat ctcgttgctc tcctggcgca ggctgatcgc
     4381 gatacgcccc gtctccggct tgcccgccgc ggcgcgcgcc tgcgggctct cgatgccgtg
     4441 caccagcgca ttgcgcagca tgtgctcgag cggcgcgccg atgcggtcca gcacgctgcg
     4501 gtccacctcc acctggctgc cctggatctc cagctcggcc ttcttgtcca gctcgcgcgt
     4561 gctctggcgc acgatgcggt acagccgctc gtcgaggatc gagaacggca ccgcgcgcat
     4621 gcgcatcagc tcctgctgca cgtcgcggct gatgcgcgcc tggtgcgtga gcgcggcatc
     4681 ggtctcgccc aggttcttgt gcagcgcctg ctggatcgag gcggcgtcgt gcaggctctc
     4741 ggccatcagg cgcgtcagtt cctgcaagcg cgtgtagcgg tcgaactcca gcggatcgaa
     4801 ctcgctgcgc tgctcctcca gcaccgacag gcgcgactgc atctggctgt cggcctggat
     4861 ctcgacctcc cgcagctgac cgcgcatccg cgcgatgctg tcgctcaggt cggcgatcga
     4921 ctgcttgacg gcccgcagct cgccctcgac gcgcgagcgc gcgatgctca cctcccccga
     4981 ctcgttgatc aggtgatcca gggtgtcggc attgatgcgc agcatcgccg ccgggctggg
     5041 cagcgggggc tcggcgcgcg cggcggcctc ggcgcgcggc gcttccgcgg ccgccggctc
     5101 gggctccggc cccgccgcca tgcgctccag gtccaggctc aggcggtcca tcttctcctc
     5161 gagctcgacg aacagctcgg gcgcgatcgc gcctgcctcg accgcggcct cgatgcggct
     5221 ctccatgagg tgcgtgagct cgccaaggcg gatcgcgccc gccatgcgcg cactgccctt
     5281 cagggtatgc agggcgcgct gcagcgactg cagcacctgc gggtccgcgg gattcgcctt
     5341 ccagtcgcgc aggtcgccgc cgatcagcgg cagcagctgc tgcgcctcct cgaggaagat
     5401 cggcagcagc tgcgcgtcga tgtcgtcgtg cagcacgcgc ttgttgcgcc cacccgcttc
     5461 cggcgccggc ttgggcgctt cggcttgcgg cctgggcttg cccggcgcgc gcaggcgcgc
     5521 cgccaggtcc tgcagcgcgc gcagcacctg cggcgccggc gtcggcgcct cgcgccgcgc
     5581 cagcgcgtcg gccatggtgc gcagggcgag gatcgccgcc tgcacggtgc cggcgtcggc
     5641 cgcgccggtc agccgcgcga gcggcatcca ttgctcgagc gcctcggcca gcgtggcgac
     5701 ctcgacgaag cccgcggtgc gcgaactgct ggcgagcgtg tgcgcggcgc gcacgaattc
     5761 gtgcgatgcc tcggcgccct gcttgcggcg ccacgccgcg cactgcgcct ccagcgaagc
     5821 ggtgtgctgg cccgcctcgg ccagataaat gtcgaacagg ctgcgcgcca ggcgcaggcc
     5881 ggcgacctcg acctcttccg gcaccggcgg cggctccgcc agctgcggct ccggctcggg
     5941 cagcaattcg gccagcggct cgggggcggg cgccgcagcc ggcgccggcg cgggcacggc
     6001 aacgggcgcg gccgccggcg cctcctcggt cttcaggcgg cgcgccagct cgacgatccc
     6061 ggcgccggtg atctccgcct gcaagcggcc ttccttgagc tgcgccaccc agtcggcgaa
     6121 cgcagcgctc gcgccctcga tcagctcgag cagcccgggc gtggcggggc gctgctgctc
     6181 cagccacttg ttcatcacct gctcgacctc ccaggccact tcgcccaggt ccatcaggcc
     6241 gaccatgcgg ccgctcccct tcagcgtgtg gaagccgcgc cggatggtgg tcaacgcatc
     6301 gcggtcctgg ggctgcgccc gggccgccgg cagcgcggcg tcgatgttcg ccagcacttc
     6361 gccggcctcc tcgaggtaga tggcgagcag ttcctcgttg acttgctgcc gcgacgccgc
     6421 cggcccggac tggggcgcga aggacgtcgg cgcggcggcg agcgaggcgg cggccgcctc
     6481 cgcgattgcc cccgcatcca ccgccgcggc gaccggcgcc ggagcggcgg cacccttcag
     6541 ggccgccgcg gtgagcacca ccgtcgagtc gtggctgagc gtttcggccg gcgcatgcgc
     6601 cttgtcgtag cgccggaaga acagctcgac cgcctgctcc gccggctcgc ggccgttcag
     6661 gcagggatcg aggaacaacc ccacgctcga caatccctcc gcgatccagt ccatcgcgtc
     6721 ccccgccggg tcgtgctccg cctgcgcgca ggcctcgatc agcttctcgc acagcggcag
     6781 gatctgcgcg gcgcgcgcga agcccagcac gttcagcgcc ccatggatct gccgcaggtc
     6841 gggcgccagc ccgggcagcg tgtcgcgctt gcccgggtcg cgcgcgaatc cgtccaggat
     6901 ctgctccacg cgctgcagat tggccgcgat ctcgcgcgcc acctgggcgc gcagctgcag
     6961 ggcgccgatg cgctcgctca gatcggcgcg cagtcccgcc ggcggctccc cggtcgattt
     7021 gccattggca gcgtcgagca gccagccgcc catgatgccg atctgctgct ccagatcggc
     7081 ggcggggtcg gtgaagttct ccaccacgct ttccgcaagc aggaaggccg acgccatttc
     7141 gatcaccagc agctgactct ggggcggaaa cgggtcgggc aggcgcgtcg tcaccagcgc
     7201 gatcgcatcg agcagcttca ccagatgcgg attgcccagc gccccggcct tggcgcggaa
     7261 cgccgccacc agctcgcgga agcgcgcggc gctcttgggc tcccccgaca cgtactgcag
     7321 ccacaggctc ttcagcgcct ccagccgcga gcgcacgtcg ctcagcgcag gctgcagcca
     7381 gtccatgtcg aactcgaccg tgcccggcgc gccctgcgcc tcggggaaca ggccgtcgag
     7441 gaggtagagc tgcttgacct ccttgacgcg cggcgtcgcc ggcttgcatt tggcgacggc
     7501 gtagagcacc tcgcgcagca aggcttcgct caccgccggc gcgccggccg ccaggtcccg
     7561 cacctggaaa tcgatcttgt tgcacagggc cttggccctg gcgagccagt ccgcatccgg
     7621 cggcgcggcg aagccctcca gcaggccggc ggcggcccac cacagggcgc gcggctcggg
     7681 cagctgcgcg gcgacgcggt gcagcgcatc gagcgcctgg cgcatctcct gcagcccgcc
     7741 gctctggttg cgcagcgctc ccagcaggcc gcgctggaac tgcgtgcgct gggcctgcag
     7801 gtacggcgcc acttccgccg cgggcaagct gcgcggctcg gggtgcgcgg gcgcctgcag
     7861 cgtgaggtcg gggtagaaca ggtccttctc cgacgcgccc ttgccgcccg gcaaggtcga
     7921 cagctcgcgg tataccggaa acaggcgcag cggcacgttg gcctggccgc gctcgagccc
     7981 gtcgacgaag tccttcagcg ccagcaccgc gcggtcgatc acgccgatcg tcgccgagct
     8041 cggacgcgcg ccgttcagcc cggcgaagcc gccctcgatc gcctcgcaga agcgcgtcgc
     8101 gcccgccagc cccaccatgc gcagcgcccc gctcacctgg tgcaggtgcc cggggcaggg
     8161 gcccaggagc gccgcgtccc cggggtcggc ggaaaacttg gcgatgtgct cccgcacctg
     8221 ccgcagcgcc tggtccacct cggcggtgat ccaggacagg atggtggcgg aagcgttgct
     8281 catggcccct taggcgagct tgaagcccga gaccgaggcc ttgaggcctt cggcgagcgc
     8341 ggtcaggcgc tcggcggagg cagcggtgcg ccgcgtgccg tcggtggtca tctgcgtgat
     8401 ggcgaggatc tcgttcatcg ccgccgccac ccgcgtggcc gatatcgact gctgctccgt
     8461 ggcgtcggag atcgagccga tcagctcggc gagctggttg gacacctgct cgatctcgcg
     8521 cagcgcgccg ccggcctcgt ccgcggtctt ggtcccgtcc accacgccgc gcgtgctgcg
     8581 ctccatcgcc tccaccgcgt cctgggtgtc gcgctgaatc gacttgacga tggcgccgat
     8641 gtgcttggtg gcctcgcccg agcgctccgc cagccgctgc acctcctccg ccaccaccgt
     8701 gaagccgcgg cccgcctcgc cggccgaggc cgcctggatg gcggcgttca gcgccagcac
     8761 gttggtctgc tcggtgatgt ccgagatgag ctgcacgatt tcgccgatct cctgcgagga
     8821 ctcgcccagc cgcttgatgc gcttggaggt ttcctggatc tgctcgcgaa tgtcgttcat
     8881 gccgcggatc gcgttctgca ccgcctgggt gccctttgcc gcggcgttga gcgaggtccg
     8941 cgccaccctg gcggagtcgc ccgcgctctt cgagacgccg gtcatcgatt gcgccatctg
     9001 caccaccgac tggccggtgc cctggatctc ggtggcctgc cgctgcgccg cctgcagcag
     9061 ctggccggac accgcctgcg cttcggcggt ggcgctggtc acctgctgcg cggcatcggt
     9121 gatgccggac accaggcgcc gcagctcgtc gatggtgtag ttgatcgagt cggcgatcgc
     9181 gccggtgatc tcctcggtga cgtgcgcctt cgcggtcagg tcgccgtcgg cgaggttgcc
     9241 gatctcgtcg agcagccgca ggatggcctc ctggttggtc cggttcagct gctcgctgct
     9301 tgcggcgcgg cggcggctgt cgtcgatgta caccttgcca accagcagca gcaccagcaa
     9361 ggcgaggacg cccatcgcac cgatgatcag gtaattgaac tggcggccgc cgagctcggc
     9421 ctcgtacgcc tggcccagcc gctcggcgga ctgcagcagc gattcgctgt cgttgaacat
     9481 ctggaacgcg gcgcgcttgg cgcccaccag ccgcgcctgg ttgcccagga tatcgctcac
     9541 cgcgcgctgg tagtcggcga acgccgcctg cagctcgccg agcttgccgc gcagctcggc
     9601 gtcctgcacc cgcgcgatgc gcatggcttc gctgccctgc agaagtccct gcagcgtgtc
     9661 gcgataggtg ttggtgtcct tgcccagcag gaacgccacc tcggggtcga cgatgttctc
     9721 cgccagcatg gtgttcgcgt tcttcgccat gcgctgcgtg agcatggtca gctgcgcggc
     9781 gatcgcgttc tgccgcgccg agccgccccc ctgcacgctc agcgccgcga tctcgtcggt
     9841 gagctcctgc agcaccgggt tgttggtgtt gatcgcgcgc accgccctgc ccagcgccac
     9901 caggttgcgc tcctcgtcga tcaccagcgc ggcgttgcgc tcggtcttgc cccattcgcg
     9961 atccagcgcg ttgagcagcg gctgcacccc ggccgaggtg ggcggcaggc tcacgccgcc
    10021 gctcgcgccg ccgaaagcga gcagcttgac cagcgcggcg aattcgtcgc ggctggcgcg
    10081 cagctgcttg aacgcctcgg tgtttccctg cgaggcctgc tgcgccgcct tcgccaggcg
    10141 ctgcgacagc atctgcagct cgcctgcggt ggcgatgtag atggtggcga aggttccctg
    10201 gcgggtgtcg tagaccacca ccgccgcgat ggcgccgagc accagcacca gcgcgccgat
    10261 cagcacctgc agctgccggc cgatcgccag ccgcccgatc agccacagcc cgccgccctg
    10321 cgctgcggcg gccgccggcg ccggcgccgg ttcggcgccc cgcccggcga atggaagttt
    10381 gagcgagccc agattgagtg ccatggcgtt cccccttagc gcaagaattt tccgccgcgg
    10441 ccgatgatcg tcaggtcgac gaacttcgat ccggcgtggt tggtcggcga gtagctcacg
    10501 aagaagccgc cgaggtcgac gctctgcatc ttctccagcg ccgcaatgta gccctcgcgc
    10561 gtcaggttgc ggccggcgcg ccgcaggccc tccaccgaca ccttggcgga gaggaagccc
    10621 tccatcgcgc tgaagttgta gtccttgaag cccgccttgg cggcgagctg ctggtattcc
    10681 ttcaccaccg gcaccgcccg gccccagggg aacggcacca cctgcgaaat cgccaccccg
    10741 acgccgtcct tgcccagggc gtcggccagc gcctggctgc cgacgaacga gacgttgtag
    10801 aaggtggctg cgctgccggc cttctgcatc tgccggatga actccgcgca ggaggtgtag
    10861 gcgctgatca tcaccaccgc gtcgggcttg gcggcgtgga tgctcttgac cgcctcctcc
    10921 accttgatgg tgttgcgctc caccgtgccg agcgccgaga tcttcatgtt gcgcttctgc
    10981 gtggcgatct ccacgccctt caggccggcc tggccgtagg cgtcgttctg gtagaacacg
    11041 gcgatgttgc gcccgccgat ggacaccacc tgttcgacga tcttgtcggt ctcgtcgtaa
    11101 tagctcgccc gcacgtggaa cacgtagcgg ttgaacggca cccgcagggc ctcggcgccg
    11161 gtgaacatcc ccaccagcgg caccttggcc tgcgtgacca ccggcaggtg caccaccccg
    11221 gtgggagtgc cgacatagcc gagcaaggcg aacactttgt gctcctcgat aagtttcttg
    11281 ctcgcgcccg gcgcgacggc gacctcgtag cggtcgtctt cggtgatcag ctcgatgcgg
    11341 cggccgtgca ccccgcccgt cgcgttcacg tgatccaggt aggacttgat gccgttgcgc
    11401 atctggatgc caagctgcgc ggccggcccc gagaacaccg ccgcctgccc gagcaggatc
    11461 ttgtccgcgg tgacgccgac ctgctgcgcc gccgcgccct gcgcgagcat ggcggcagca
    11521 gcggcaatca cccgccgtat cgtgtttcgc ttcatgcctt cctccgattc gctttacggt
    11581 tcacgcgccg accgcgagaa atcccgggtc cgcgaccagt tgcgccacgt ccagttcctt
    11641 ccagcgttcg ccctgcgcgt cgctgtactc gcccttcagc caggccgcct gcggcccgct
    11701 cgccgactgc ggctgcaatt cctccgcgtt gcgcaggccg agggagcggt ccaccagcag
    11761 cgcgccgccg gtgcggaagc gcgcgccgaa cagcaccagg cgcgcctgct cgccgggcgg
    11821 cgccgggctg gcccccagga aggccgcgaa gtccaccacg ctgcacaggt tgccccggat
    11881 gttggccacg ccgcggaacc acggctgcgt cagcggcacc ggcgtgatcg gcggcaccgg
    11941 gatcacctcg cccgcctcgg cgagatcgat cagccagcgc tgcgcgccga cctgcaggcc
    12001 gagcttcgag gcgttgcggg cgctgtccgc cgcgcgcagg cgttcggcga gctcgcgctg
    12061 gtagtcccgg agagagattc tttgcgccat cagcccaggg cggaaatctt ggcgagcaac
    12121 tcggggccct tcaccggctt cacgatgtag tcgcgcgccc cctgcttcat g
//
LOCUS       DASRIE010000115         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1946323, whole genome shotgun sequence.
ACCESSION   DASRIE010000115 DASRIE010000000
VERSION     DASRIE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946323"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFB53_05085"
     CDS             complement(<1..372)
                     /locus_tag="VFB53_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HZN86146.1"
                     /translation="MRKLYLRIYFAVLASLAAFALGAAVLWHTLGEPRGMDQPWATIA
                     RNVLPSASAPPAEQQAALERVARDLPFDLSLFTADGARLAAVGAPLGAPERAGWQGGP
                     SGPRWAAKLPDGRWLVARSPHG"
     gene            complement(373..1056)
                     /locus_tag="VFB53_05090"
     CDS             complement(373..1056)
                     /locus_tag="VFB53_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN86147.1"
                     /translation="MAERILLIDDDARLAAMISEYLGAAGYRVTTAPSGAAGLARLAA
                     DPYDGLVLDLSLPDMDGLEVCRQLRARWDLPVLMLTARGEATDRIVGLELGADDYLPK
                     PFEPRELLARLRAILRRRASGASSLLRFGRLDIDRDARAVRLAGVETGLTSFQFALLV
                     ALAENAGRVLSRDTLMDLLRGEKLEAFDRSIDVHVSRIRAAIEDDPKKPRRIITVRGA
                     GYVFAKQQS"
     gene            complement(1127..1594)
                     /locus_tag="VFB53_05095"
     CDS             complement(1127..1594)
                     /locus_tag="VFB53_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZN86148.1"
                     /translation="MNKFRRITLATLAGGIAGAVGFKALAHGRGHGSGPIDPADLERH
                     LERMLKHLYVEIDATEAQKQQLEPIVKQAARDLLPVRERLQAARREALELLARERIDP
                     AALETLRAKQIGLADEASRRLTRAIGEAAEVLTPAQRKELAAHFARRHGRWHG"
     gene            complement(1591..>2219)
                     /locus_tag="VFB53_05100"
     CDS             complement(1591..>2219)
                     /locus_tag="VFB53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN86149.1"
                     /translation="SCSMSRAGATAAAMALAFAAVSAGCGSWSGFGFRADGPEMSRLR
                     QVLALKESMVVADVGAGKGELTQALAKEVGSGGRVFSTEIDPSRLARLRETAGHNVVV
                     VEGRSGESGLAPGCCDAIVLRRVYHHLSDPSGFNASLRQALRPGGVLVIIDLPPPFSW
                     LRGSLGVPAQAVIDEVRGGGLELVRLIDDWPGRGPLQTYCAVFKKGVS"
BASE COUNT          329 a          833 c          762 g          295 t
ORIGIN      
        1 cccatgcggc gagcgcgcga ccagccagcg cccgtcgggc agcttcgcgg cccagcgcgg
       61 accgctcgga ccgccctgcc agccggcgcg ctcgggcgcg cccagcggcg cgccgaccgc
      121 ggcgaggcgc gcgccgtcgg cagtgaacag cgagaggtcg aacggcagat cgcgcgccac
      181 gcgctccagc gcagcctgct gctcggcggg cggcgcggac gccgacggca ggacgttgcg
      241 cgcgatcgtg gcccatggct ggtccatgcc gcgcggctcg ccaagcgtgt gccagagcac
      301 cgcggcgccg agcgcgaacg ccgccaggct cgcgagcacg gcgaaataga tgcgcagata
      361 cagcttgcgc atctagctct gctgcttggc gaagacgtag cccgcgccgc gcacggtgat
      421 gatgcggcgc ggctttttcg ggtcgtcctc gatcgctgcc cgtatccgcg acacgtgcac
      481 gtcgatggag cgatcgaacg cttccagctt ctcgccccgg agcaggtcca tcagcgtgtc
      541 acgcgacagc acccgccccg cgttctcggc cagcgccacc agcagcgcga actggaacga
      601 cgtcagacct gtttcgaccc ccgccagccg caccgcgcgc gcatcccgat cgatgtcgag
      661 gcgcccgaag cgcaggagcg acgatgcccc ggaggctctg cggcgaagaa tggcgcgcaa
      721 gcgggccagg agctcgcgcg gctcgaacgg cttgggcagg taatcgtcgg cgccgagctc
      781 gagaccgacg atccggtcgg tcgcctcgcc gcgcgcggtg agcatcagca ccggcaggtc
      841 ccagcgcgcg cgcagctgcc ggcacacttc gagcccgtcc atgtcgggca gcgacagatc
      901 gagcaccagc ccgtcgtacg ggtcggcggc gagccgcgcg aggccggcag caccgctggg
      961 cgcagtggtg acgcgatagc ccgccgcgcc gaggtactcg gagatcatcg cggcgaggcg
     1021 ggcatcgtca tcgatcagca ggatgcgctc ggccacgcgc gcactataac catctgcgca
     1081 ccgaatctca gcggcggccg cagccagccg gatttggcga ccggcgtcag ccgtgccacc
     1141 gcccgtggcg tcgcgcgaag tgcgcggcca gctccttgcg ctgcgccggc gtgagcactt
     1201 cggccgcctc gccgatcgcg cgcgtcagcc ggcgcgaggc ctcgtcggcg agcccgatct
     1261 gcttcgcgcg cagcgtctcg agcgcggccg gatcgatgcg ctcccgcgcg agcagctcca
     1321 gcgcctcgcg gcgcgccgcc tgcagccgct cgcgcaccgg cagcaggtcc ctcgccgcct
     1381 gcttgacgat cggctcgagc tgctgcttct gcgcctcggt ggcgtcgatc tcgacgtaca
     1441 ggtgcttcag catgcgctcg agatgccgct ccagatccgc cgggtcgatc ggcccggacc
     1501 catgcccgcg gccgtgcgcg agcgccttga agccgaccgc gccggcgatg ccgccggcga
     1561 gcgttgcgag cgtgatgcgc ctgaacttgt tcatgagact ccttttttga acaccgcaca
     1621 gtaggtttgc agggggccgc ggcccggcca gtcgtcgatc aaccgaacca gctcgaggcc
     1681 gccgccccgg acttcatcga tcacggcctg cgccggaaca ccgagggagc cgcgcagcca
     1741 ggaaaacggc ggcggcaggt cgatgatcac cagcacgccg cccgggcgca gcgcctgccg
     1801 caggctggcg ttgaagcccg atgggtcgct caggtggtga tacacgcggc gcaggacgat
     1861 ggcgtcgcag cagcccggcg ccagtccgct ctcaccgctt cgcccctcca ccaccacgac
     1921 gttgtggccg gcggtttcgc gcagccgcgc cagacgcgag ggatcgatct ccgtcgagaa
     1981 cacgcgcccg ccggagccga cttccttcgc gagcgcctgg gtgagctcgc ctttgccggc
     2041 gccgacatcg gccaccacca tgctttcctt cagcgccagc acctgccgca ggcgcgacat
     2101 ttcgggtccg tcggcgcgga aaccgaatcc gctccagctt ccgcagccgg ccgaaaccgc
     2161 cgcgaaagcg agcgccattg ctgctgccgt tgcgcctgct ctgctcatcg agcacgagc
//
LOCUS       DASRIE010000116         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3242627, whole genome shotgun sequence.
ACCESSION   DASRIE010000116 DASRIE010000000
VERSION     DASRIE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242627"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFB53_05105"
     CDS             complement(<1..616)
                     /locus_tag="VFB53_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN86150.1"
                     /translation="MRSLVAALALGMLCAAAQAQYPNRPIRLIVPFPPGGAAELGARI
                     FTQPLSLALGQPVIIETKPGADGAIAADATMKASPDGYTLFYATNTAFSWVPAVRKNP
                     PYDPVADFTPVSRIGTFGFFIFSHPSVPANSMAELLAHLRANPGKLNYGSGNSTAMLS
                     TAQLVQQEKLDVVHIPYKGDGPLFVDLLGGRVQFAISTPGAAAPQ"
     gene            643..1578
                     /gene="gnd"
                     /locus_tag="VFB53_05110"
                     /pseudo
     CDS             643..1578
                     /gene="gnd"
                     /locus_tag="VFB53_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            1590..2051
                     /locus_tag="VFB53_05115"
     CDS             1590..2051
                     /locus_tag="VFB53_05115"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZN86151.1"
                     /translation="MGVSGCGKSTVGALLAQRLGCEFLDGDEFHPPENVAKMASGVPL
                     TDADRRPWLERLNAELRKRENAVLACSALKQSYRDILAQGADCRFVHLRGPIELIRSR
                     LEARRHRYMPASLLESQFAALEPPARAIDIDVARPAADCVERILAQLPMCA"
     gene            complement(2030..2488)
                     /locus_tag="VFB53_05120"
     CDS             complement(2030..2488)
                     /locus_tag="VFB53_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86152.1"
                     /translation="MKSAKTLLASAAIAALSGCYVVPGPDGQLWHVIPGHASPGSVPP
                     LAAAGAPGPVSLNVRLYPANDTATRTGVVTGTVINMMTGKGRFQLNYNGETLSGEATR
                     VDGDARRGVASAYGPSGIYMSCEYQMSNPRQGAGTCTLSNGAKYQAHIGS"
     gene            complement(2574..>5432)
                     /locus_tag="VFB53_05125"
     CDS             complement(2574..>5432)
                     /locus_tag="VFB53_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86153.1"
                     /translation="GDADPATFFYYLGLAAADLEDGDHAPLPLLTPEYHAGLLAFTRR
                     YAQELYARLKPPFVIVFDGYHEVPATSPLHEAMREALRELPPGGRAIVVSRVDPPGTL
                     ARLRANRQLAHIGWEELRLTREETVSIAGRRRPELAPEASAALYEKTQGWAAGLVLLL
                     EQARMGVPIAQAPDLATRQLVFDYLAGEILQKSDARTQEFLLVTAQLAQMTPALAQAL
                     SGDAMAGETLARLHRENYFVARREAHGEPLYQFHPMFREFLLARAQEALPKERRRQLQ
                     KEAAALMEQAGQVEEAVALAREGHDWEAMARLIERHAGAMYDQGRAETLVQWVEELPP
                     EVRQAHPWTLYWAAACQAQRAPREGRLGFERAFELFRAQELPDEAGMVLACSGAMYAI
                     LYELDDFSLLDRWIGVLDDMARAGARLPAPEAEARVAASMFISLTLRQPQRRDIQQWV
                     ERALAASREVPDPNLKLFVGLLAALTLMWTGVHAKARGLLEAMRRLADAPGVTPFSVI
                     TLKNVEAMYHMLNAEREPCLAAMREGLELARAAGVHTWSFQLLVYGYGGALGSGDLDA
                     AAPLARQLEGQAAGAGRFNLCLLHHFQAWEAMLRKDPMRALQQEKTALRMAIEVGCPF
                     VEVLTRLAIAQVLAECGDERKCVSHLREMRALARDIDSRHLEYACLLGFADIALEHGR
                     QRPGLNALRRGFALGREHGYQHFLWWRPSAMARLCAHALAAGAEAEYAKHLVKRRALA
                     PEQPPLEVPEWPWHFRVQTLGGFRLLRHGEPLAAAGAKAQRRPLDLLKVLIAHGGERV
                     AEELVTDALWPRIDGDSAHRSFTSTLHRLRKLLGEDRALVLHEGKLSLDARFFWTDTW
                     ALERLLAEIDGAGKSPLAPEDAERLGARLLALYRGPFLAREAAETWSLRPRERLHARF
                     ARALGQLGRYWEESGRPERARDLRERSQDAESAALSDR"
BASE COUNT          733 a         1960 c         1974 g          765 t
ORIGIN      
        1 cctgcggcgc ggcggcgccc ggcgtcgaga tggcgaactg cacgcgcccg cccagcaggt
       61 cgacgaacag cggcccgtct cccttgtagg ggatgtgcac cacgtcgagc ttctcctgct
      121 gcacgagctg cgccgtggag agcatggcgg tgctgttgcc gctgccgtag ttgagcttgc
      181 ccgggttggc gcgcaggtgc gcgagcagtt cggccatgct gttcgccggc acgctcgggt
      241 ggctgaagat gaagaagccg aaggtcccga tcctggaaac cggcgtgaaa tcggccaccg
      301 ggtcgtaggg cgggttcttg cgcaccgccg gcacccagga gaacgcggtg ttggtggcgt
      361 agaacagggt gtagccgtcc ggcgacgcct tcatcgtcgc gtccgcggcg atcgcgccgt
      421 cggcgcccgg cttggtctcg atgatcaccg gctggccgag cgccagcgac agcggttgcg
      481 tgaagatgcg cgcgccgagc tcggcggcgc cgcccggcgg gaacggcacg atcaggcgga
      541 tcggcctgtt cgggtactgc gcctgcgcgg ccgcgcacag catgccgagc gcgagcgcgg
      601 cgacgagact tctcatggga cacccctcct cgcggcgatt atagaatgag cccgtcgcgc
      661 ggaggcagca tggacatcgg catcgtgggc ttgggacgga tgggcgccaa catggcgcgg
      721 cggctggcgc gcggcggcgt gcgcgtgctc gcctacgacc gcgagcgcgc ggcgcgcgag
      781 gcgctcgcgg gcgaggcgga cgtcgcctgc gtcgaaggcc tcgccgcgct gtgcgcgcag
      841 ctcgagggcg agcgcgtgct gctgctgatg ctgcccgccg gcgccgccac cgaggacacg
      901 ctgcgcgagc tgctgccgct gctctcctcg ggcgacacgc tggtcgacgg cggcaacgcc
      961 ttctacaagg attcgatgcg ccgcgcgatg gaactgtcgc agcacggcct gcgctacatc
     1021 gacgcgggcg tctcgggcgg cgtgcacggc ctcgccaacg gctactgcct gatgctcggc
     1081 ggcacgccga agtcgatcga gctgttcgag ccctacgcgc gcgtcctcgc gccgggcccg
     1141 gagcgcggct ggctgcactg cggcccggcg ggcgccggcc actacgccaa gatgatccac
     1201 aacggcatcg agtacgggat gatgcaggcg ctcgccgagg gcttcgccct gctcgaggcg
     1261 cgccaggacc tgggcttcga cgccgggcgc ctggccgaga cctggcgcca cggcagcgtg
     1321 atccgctcct ggctgctcga cctgtgcgcg gaagtcctgc agcgcgacga cgcgctgcag
     1381 ggcgtggcgc cggtggtcgc cgactcgggc gaaggacgct ggacggcgct cgagtcgatc
     1441 gagctcgggg tgcccgcgcc ggtgatcacc atggcgctga tggggcgctt ctccagccag
     1501 ggacgggcgg actacgcgcg caagctcctc gcgatgatgc gcgcgggctt cggcgggcac
     1561 gccgtcacca aatcttgagg atcgtcgtaa tgggcgtgtc cgggtgcggc aagagcaccg
     1621 tcggcgccct gctcgcgcag aggctcggct gcgagttcct cgatggcgac gagttccatc
     1681 cgccggagaa cgtggcgaag atggcctccg gggtgccgct caccgatgcg gatcgccggc
     1741 cctggctgga acgcctgaac gcggaactgc gaaaacgcga aaacgcggtg ctcgcctgct
     1801 ccgcgctcaa gcagtcctac cgcgacatcc ttgcgcaggg cgccgactgc cgcttcgtgc
     1861 acctgcgcgg accgatcgag ctgatccgct cgaggctgga agcgcgccgc caccgctaca
     1921 tgcccgcgtc gctgctggaa agccagttcg cggcgctgga gccgccggcg cgcgccatcg
     1981 acatcgacgt ggcgcggccg gcggccgatt gcgtcgagcg gatcctcgct cagctgccga
     2041 tgtgcgcctg atacttggcg ccgttggaca gcgtgcaggt gccggcgccc tggcgcgggt
     2101 tgctcatctg gtactcgcag ctcatgtaga tgccggaagg cccgtaggcg ctcgccacgc
     2161 cgcggcgcgc atcgccgtcc acgcgcgtcg cctcgccgct cagggtctcg ccgttgtagt
     2221 tgagctggaa gcgccccttg ccggtcatca tgttgatcac cgtgccggtg accaccccgg
     2281 tgcgcgtggc cgtgtcgttg gccgggtaga gccgcacgtt gagcgacacc gggccgggcg
     2341 cgccggcggc ggcgagcggc ggcacgctgc ccggcgaggc atgcccggga atcacgtgcc
     2401 agagctgccc gtccggcccg ggcacgacgt agcagccgga aagcgcggcg atcgccgctg
     2461 aagccaacag cgtctttgcg gatttcatgt ttccccctgt ctaggttcgc aggcgaagtc
     2521 cgcctgcaag cccagcatgg gaggagccga cctgcttccg gcctaccggt cgcttaccga
     2581 tcgcttaagg ctgcgctttc ggcgtcctgg ctgcgctcgc gcaggtcgcg cgcgcgctcc
     2641 ggccgccccg actcctccca gtagcggccg agctggccga gggcgcgcgc gaagcgcgcg
     2701 tgcagccgct cgcgcggacg caggctccag gtctccgcgg cctccctggc gagaaacggg
     2761 ccgcggtaca gcgccagcag ccgcgccccc aggcgctcgg cgtcctccgg cgcgagcggc
     2821 gacttgcccg cgccgtcgat ctccgccagc aagcgctcga gcgcccaggt gtcggtccag
     2881 aagaagcgtg cgtccagcga gagcttgccc tcgtgcagga ccagcgcgcg gtcctcgccg
     2941 aggagcttgc gcaggcggtg cagcgtgctg gtgaaggagc ggtgcgccga atcgccgtcg
     3001 atgcgcggcc acagcgcgtc ggtcaccagc tcctcggcga cgcgctcccc gccgtgggcg
     3061 atcagcacct tgagcaggtc cagcggccgg cgctgcgcct tggcgcccgc ggcggcgagc
     3121 ggctcgccgt gccgcagaag ccggaagccg cccagcgtct gcacgcggaa atgccacggc
     3181 cactcgggca cctcgagcgg cggctgctcg ggcgcgagcg cgcggcgctt caccaggtgc
     3241 ttcgcgtact cggcctcggc gcccgcggcg agggcatgcg cgcacaggcg cgccatcgcc
     3301 gatggccgcc accacaggaa atgctggtag ccatgctcgc gccccagagc gaagccgcgg
     3361 cgcaacgcgt tcagccccgg gcgctggcgg ccgtgctcca gcgcgatgtc ggcgaaaccg
     3421 agcaggcagg cgtattcgag gtgccggctg tcgatgtcgc gcgccagggc gcgcatctcg
     3481 cgcaggtgcg agacgcactt cctctcgtcg ccgcactcgg ccagcacctg cgcgatcgcg
     3541 agacgcgtca gcacctcgac aaaggggcaa ccgacctcga tcgccatgcg cagcgcggtc
     3601 ttctcctgct gcagcgcgcg catggggtcc ttgcgcagca tcgcttccca ggcctggaaa
     3661 tgatgcagca ggcacaggtt gaagcggccc gcgcccgccg cctgcccttc gagctggcgc
     3721 gccagcggcg ccgcggcgtc caggtcgccg ctgcccaggg cgccgccata gccgtatacc
     3781 agcagctgga agctccaggt gtgcacgccc gcggcgcgcg cgagctccag gccttcgcgc
     3841 atcgcggcaa ggcagggctc gcgctccgcg ttgagcatgt ggtacatcgc ctcgacgttc
     3901 ttcagcgtga tgacggagaa cggcgtcacg cccggcgcgt cggcgagccg gcgcatggcc
     3961 tccagcaggc cgcgcgcctt ggcgtgcacc ccggtccaca tcagcgtgag cgcggcgagc
     4021 agcccgacga acagcttcag gttcggatcg ggaacctcgc gcgaggccgc cagcgcccgc
     4081 tcgacccatt gctggatgtc gcgccgctgg ggctggcgca gcgtgagcga gatgaacatg
     4141 ctcgccgcga cgcgcgcctc ggcctccggc gcgggcaggc gggctcccgc gcgcgccatg
     4201 tcgtcgagca cgccgatcca gcggtcgagc agcgagaagt cgtccagctc gtagaggatg
     4261 gcgtacatcg cgcccgagca ggcgagcacc atgcccgcct cgtcgggcag ctcctgcgcg
     4321 cggaacagct cgaaggcgcg ctcgaagccc aggcgcccct cgcgcggcgc gcgctgcgcc
     4381 tggcacgcgg ccgcccagta cagcgtccag ggatgcgcct ggcgcacttc aggcggcagt
     4441 tcctcgaccc actgcaccag cgtctcggcg cgcccctggt cgtacatggc gccggcgtgc
     4501 cgctcgatga gccgcgccat cgcctcccag tcgtggcctt cgcgcgcgag cgcgaccgcc
     4561 tcctccacct gccccgcctg ttccatcagc gcggcggctt ccttctgcag ctggcggcgc
     4621 cgttccttcg gcagggcctc ctgcgcgcgc gcgagcagga actcgcggaa catcgggtgg
     4681 aactggtaca gcggctcgcc gtgcgcctcg cggcgcgcga cgaagtaatt ctcgcggtgg
     4741 agccgcgcca gcgtctcgcc cgccatggcg tcgcccgaga gcgcctgcgc gagcgccggc
     4801 gtcatctgcg cgagctgcgc cgtcacgagc aggaattcct gcgtgcgcgc gtcggatttc
     4861 tgcaggatct ctcccgccag gtagtcgaac acgagctggc gcgtcgccag gtcgggcgcc
     4921 tgcgcgatcg gcacgcccat gcgcgcctgc tcgagcagca gcaccaggcc cgccgcccag
     4981 ccctgcgtct tttcgtacag cgcggcgctc gcctcgggcg cgagctccgg ccggcgccgg
     5041 ccggcgatcg acaccgtctc ctctcgcgtc aggcgcagct cctcccagcc gatgtgcgcg
     5101 agctgccggt tggcgcgcag gcgcgcgagc gttccgggcg ggtcgacgcg gctcacgacg
     5161 atggcgcggc cgcccggggg cagctcgcgc agcgcttcgc gcatcgcctc gtgcaggggc
     5221 gaggtggccg gcacttcgtg gtagccgtcg aagacgatga cgaaaggcgg cttcagccgc
     5281 gcgtacagct cctgcgcgta gcggcgcgtg aaggcgagca ggcccgcgtg gtactcgggc
     5341 gtgagcagcg gcagcggcgc gtgatcgccg tcctcgaggt ccgcggccgc cagacccagg
     5401 tagtagaaga aggtcgccgg gtccgcgtcg cc
//
LOCUS       DASRIE010000117        18076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1690405, whole genome shotgun sequence.
ACCESSION   DASRIE010000117 DASRIE010000000
VERSION     DASRIE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690405"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFB53_05130"
     CDS             complement(<1..593)
                     /locus_tag="VFB53_05130"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZN86154.1"
                     /translation="MSVAHPLAPEQRSVGLRDLAWLVGALLLVVAPHALRMPWWLTLL
                     VLTLYAWRAQIALAGARLPSHWLVLGIALLSIGAVFLEYRTLFGRTSGIVLLALFSGL
                     KLMELRTHRDAAVTAFLCYFLVMTNLLYSQSIPTALLAGAALLAVTATLAGLSAPRRP
                     MRANLRTAGFLLAHAAPAALVLFLLFPRVQGPLWGLP"
     gene            complement(590..1549)
                     /locus_tag="VFB53_05135"
     CDS             complement(590..1549)
                     /locus_tag="VFB53_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN86155.1"
                     /translation="MMRALGGGARLRQWFYGYAAPERGTVVLGHRRVYIVPTRLGWLF
                     GATLVILLIGSINYALQLGFALTFLLAGLGLAAMVHTTRNLARLAVSAGRAEPVFAGE
                     AAQFRLYLDNRARYDRPAVLVRHQASGAQVVLELPAGAIEEAVLAVPTARRGWLGLTR
                     VMLETRFPLGLFRAWSYVEPDLRCLVYPRPEHAPLPAPAQDAAAGTARTHARGNDDFS
                     GLRGYQPQDPPRHVAWKAVARADVMLTKQFSGAAGVELWLDWGDLPALDAERRLSRLA
                     GWVLAAERAGARYGLRLPGLEFAPGSGDAHRVACLQALALHEA"
     gene            complement(1551..2159)
                     /locus_tag="VFB53_05140"
     CDS             complement(1551..2159)
                     /locus_tag="VFB53_05140"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZN86156.1"
                     /translation="MTRAGALATALAAALASPLAAQAGAQIYEPLAESLRGRMSAAIA
                     DKSVPELLFQEQGDGNRWIEEMSRRLEKRIADRKQRLELLRTIHFEAQRAGLDPQLVL
                     GVIEVESNFRKYAVSRSGARGYMQVMPFWIKLVGARDHNLFHLRTNLRYGCLILRHYL
                     DMEKGDYFRALGRYNGSLGKPDYPRAVLAAWKVRWGYRGATS"
     gene            complement(2156..3883)
                     /locus_tag="VFB53_05145"
     CDS             complement(2156..3883)
                     /locus_tag="VFB53_05145"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZN86157.1"
                     /translation="MRTSRFHLGTIKEAPADAELASHRLMLRAGYIRRLAAGIYTWLP
                     LGLRVLRKVEAVIREEMNRAGAIELSMPAVQPGELWRESGRWQAYGPELLRFKDRHER
                     DFVIQPTSEEVITDLARAELKSYRQLPVHFYQIQTKFRDERRPRFGVMRGREFVMKDG
                     YSFHADYAGLEREYRSMYDTYTRIFTRLGLQFRAVAADTGAIGGTGSHEFQVLADSGE
                     DAIAYSAESGYAANVELAEALAPAAPRRAPGEAMRKVATPGKTSCAEVAALLKLPLER
                     TVKAIALVHEAQFHLLLLRGDHALNEVKAQKAIGAFRFASEAEIRQALGGPAGYIGPP
                     LAGSGPAVATAARIVADRTVAAMSDFVCGANAEGFHLAGVNWGRDLPEPSLVADIRSV
                     MDGDPSPDGKGRLKIQRGIEVGHIFQLRTKYSAAMQATVLDDKGAAHPMEMGCYGIGV
                     TRVVAAAIEQAHDERGILLPAPLAPFGACLVPIGYAKNETVRAAADRLYAELQAAGIE
                     VLLDDRDERPGVLFADMDLIGIPHRVVLSERGLAAGEAEYKSRRAAQPQGVPLAQLAA
                     FLKERLAAA"
     gene            3959..4468
                     /locus_tag="VFB53_05150"
     CDS             3959..4468
                     /locus_tag="VFB53_05150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZN86158.1"
                     /translation="MLDRDGYRPNVGIILANGRNEVFWGKRANQHAWQFPQGGIKHGE
                     SPEQAMYRELEEETGLKADHVRVLGRTRQWLRYEVPEKWVRRDWRGTYRGQKQIWYLL
                     RMVGRDCDVKLRTSDKPEFDAWRWHEYWVPLEAVIEFKREVYREALAELHHYLGVDRG
                     GRGARASTR"
     gene            4496..5113
                     /locus_tag="VFB53_05155"
     CDS             4496..5113
                     /locus_tag="VFB53_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN86159.1"
                     /translation="MDRQYRELPIRELGPDAGFRRPQQPLPARVTLESSALEVMTDLS
                     RVSPATIRPQAPLEGANQFMISRGVRLLLVIDVRDTVLGVLTATDVLGEKAMRVSVER
                     GAKRSELTVADLMTPAERVEVIALEDLQGARVGHLLETLRRSGRQHALVVDHDGAAPA
                     APPVRRAMVRGIVSLSQLARQLGLLLPQAGEVARTFAEIEAALGR"
     gene            5232..5654
                     /locus_tag="VFB53_05160"
     CDS             5232..5654
                     /locus_tag="VFB53_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZN86160.1"
                     /translation="MKPLKGTKTHQHLKDAFAGESQANRRYLYFAAKADVEGQNDVSA
                     LFRSTAEGETGHAHGHLEYLEQVGDPATNLPIGRTRDNLKSAVAGETHEYTDMYPGMA
                     KDARGEGFAEIADWFETLAKAERSHANRFQKALDALAD"
     gene            5657..6238
                     /locus_tag="VFB53_05165"
     CDS             5657..6238
                     /locus_tag="VFB53_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HZN86161.1"
                     /translation="MARIERGSLLSLEAYARERHAFRARVIEHKRRRTVHVGEHVTLI
                     FEDELTIRYQVQEMLRIERIYEEDGIRHELEAYNPLVPDGGNLKATMMLEYPEVAERQ
                     RALARLKGVEDRVWLQVAGGARVYAIADEDLERENAEKTSAVHFLRFELGAEERQALR
                     RGAGLAIGIDHPQYQASVDASSPVRDALAADLA"
     gene            6304..6768
                     /locus_tag="VFB53_05170"
     CDS             6304..6768
                     /locus_tag="VFB53_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HZN86162.1"
                     /translation="MSDWERRQQGREQPEAAVTLPAFPRPSALLPFFVSAASDFRFFV
                     DGASLSVGGDGVVRYTLVARSPQGAETVVYEGMRCATREVRGYAAGRADGSWSRRDTP
                     WRAIGDPSVQRWHRALATEYLCPGKVPIQDAAEGVDALRRGGHPRAVHRAGG"
     gene            complement(6765..7883)
                     /gene="proB"
                     /locus_tag="VFB53_05175"
     CDS             complement(6765..7883)
                     /gene="proB"
                     /locus_tag="VFB53_05175"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZN86163.1"
                     /translation="MPGQLASARTLVVKVGSALVTNEGRGLDVAAIARWAAQIAQLRA
                     LGRRCVLVSSGAIAEGLQRLGWTRRPHALHELQAAAAVGQMGLVQAYETCFRKHGLAT
                     AQVLLTHADMADRQRYLNARSTLLTLLELGVIPVINENDTVVTDEIKVGDNDTLAALV
                     TSLVEADALVILTDQAGLHDADPRLVPGARLIEEATAGEARLEAMAGGAGSALARGGM
                     ITKVLAAKRAARSGAHTVIASGREPDVLVRLGRGERIGSLLLSQTTPLAARKQWLADQ
                     VTVGGTLALDAGAARALLQGGKSLLPIGVSAVQGDFERGAVVACLGPEGREIARGLAN
                     YSAAESRLILRRPSSEIESVLGYVDGPELIHRDNLVLV"
     gene            complement(7889..8929)
                     /gene="obgE"
                     /locus_tag="VFB53_05180"
     CDS             complement(7889..8929)
                     /gene="obgE"
                     /locus_tag="VFB53_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZN86164.1"
                     /translation="MKFIDEANIEVHAGKGGDGSAAFRRERYVPRGGPSGGDGGRGGS
                     VWAVADRNINTLIDYRFARIHRAEHGDAGHGSDQYGRGGRDVELRVPVGTLVRDAGNG
                     ELIADLARDGERAQLARGGQGGLGNLHFKSSTNRAPRQFTRGLPGESRKLALELRVLA
                     DVGLLGLPNAGKSTLVRAVSSARPKVADYPFTTLNPSLGVVRVAAGASFVIADIPGLI
                     EGAAEGAGLGHQFLRHLSRTRLLLHLVDIAPLAADADPARDARALALELKKYDPALHR
                     KPRWLVFNKIDLAADADARIARLLRKLRYKGPWFAISAIGGKGCDALCSAAYRFLAGG
                     ARQDKARARRAA"
     gene            complement(8994..9251)
                     /gene="rpmA"
                     /locus_tag="VFB53_05185"
     CDS             complement(8994..9251)
                     /gene="rpmA"
                     /locus_tag="VFB53_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZN86165.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKTYGGEQVNAGQILVRQRGTV
                     VHPGRNVGMGRDHTLFARTAGKVQFAVHGAHSKKTVSIVPA"
     gene            complement(9267..9584)
                     /gene="rplU"
                     /locus_tag="VFB53_05190"
     CDS             complement(9267..9584)
                     /gene="rplU"
                     /locus_tag="VFB53_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZN86166.1"
                     /translation="MYAIIKSGGKQYRVQSGEQLRVESLAAEVGAAVAFDQVLMVGAG
                     DAVRVGAPLVAGAQVKATVLAHGRGDKVRIFKLRRRKHYQKSQGHRQNYTEVRIDDIV
                     AGA"
     gene            9689..10672
                     /locus_tag="VFB53_05195"
     CDS             9689..10672
                     /locus_tag="VFB53_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZN86167.1"
                     /translation="MPVTEKSASAILSPVAEDMRQVDALIRERLDSDVALVRQVAEYI
                     VAGGGKRLRPALLLLAAGAVGFRGAARLELAAVVEFIHTATLLHDDVVDESQLRRGRR
                     TANAAYGNAASVLVGDFLYSRAFQMMVGLQSMRVMRVLADATNTIAEGEVLQLMNCHD
                     PEVGEERYLDVVRRKTAKLFEAAARLGGILGGAAPDLEEGLAAYGMHLGTAFQVVDDV
                     LDYSGAAQETGKSLGDDLAEGKPTLPLIRAMQAGSPAERALVRRAIIEGGRADFAPVL
                     AAIRSTGALDYARAAAQREAAVAAQAIAGLPASAHRDSLLELASFSVTRRS"
     gene            10686..10762
                     /locus_tag="VFB53_05200"
     tRNA            10686..10762
                     /locus_tag="VFB53_05200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10720..10722,aa:Pro,seq:cgg)
     gene            10825..11049
                     /locus_tag="VFB53_05205"
     CDS             10825..11049
                     /locus_tag="VFB53_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HZN86168.1"
                     /translation="MRLILLALAVLALAWLVRRALRARRADAAPPGGAPGELVACAHC
                     GVNLPRAEARAAAQGLYCSEEHARLGPRRP"
     gene            11049..12641
                     /locus_tag="VFB53_05210"
     CDS             11049..12641
                     /locus_tag="VFB53_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86169.1"
                     /translation="MATLAYAPLFAREDRPPDAFWISLQYFNLYRIVVALLFLVATYF
                     TYEPINFGAHDLRLFRAGCVGYLALGIALQIVLRSVRERFNLQLSAHALLDVIAIPLL
                     MYASGGMRSGLGVMLLISITGAGLVAPLRLNFLYAALATIAVLLEEAYWVLALDLPAA
                     SFVPPALLSMGYFATAGATGWLAQRLAANERLARERGRALANQTRVNQLVIADMQDGV
                     LVLDRQGRVVEHNPQAMRLLGMDALRGLALGAVLPGLEARWLAWRDAGARAAVAIDLE
                     ARGKEVRARLLDTGTEDRFTVVFVEDMTRSREHAQQLKLAALGRLTANIAHEIRNPLS
                     AISHASELLDEERRDEDRARLTRIIHDNTRRLERLVADVLQLNRRDRIAAERIALGAW
                     LPAFLEEFGANEAVPAGRIAIEPGRGVWMEFDREHLRQVLWNLLRNAVRHARAEPGSV
                     RLAARAFGDQVELNVTDNGPGVPESVQGQLFEPFFTTDSKGTGLGLYLARELSAANRA
                     LLEYVDDRAGAHFRIVCRGGAL"
     gene            12641..14020
                     /locus_tag="VFB53_05215"
     CDS             12641..14020
                     /locus_tag="VFB53_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZN86170.1"
                     /translation="MRRADRKPAQRERVVLVVDDEPDIRELLELTLARMGLGVVAVGS
                     IAEAREQLRTGRFELCLTDMRLTDGEGLDLVRHIGSLDNDLPVAVITAHGSAENAVAA
                     LKAGAFDYVSKPVGLEQLRALVKSALSLPDQGDEPPRDLPLLGASAPLAQVRELIGKL
                     ARTQAPVYVCGESGSGKELAARLIHGQGARRERPFVPVNCGAIPEALMESEFFGYKKG
                     AFTGAEQDRDGFFQAADGGTLFLDEVADLPLAMQVKLLRAIQEKRVRKVGATQEDPVD
                     VRIISATHQDLARLVEAARFRQDLFYRLNVIELKMPPLRECREDIATIARSILERIAR
                     QAGAVAARLSAAALAELAAYDFPGNIRELENILERATALSAGGEIGPEDLRLKPAAAE
                     DAGARAAEGALGAPLPEYLDRVEREAILQALARTNFNRTAAAKLLGVTFRTLRYRMQR
                     LGIKDENRH"
     gene            14004..14552
                     /gene="ampD"
                     /locus_tag="VFB53_05220"
     CDS             14004..14552
                     /gene="ampD"
                     /locus_tag="VFB53_05220"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HZN86171.1"
                     /translation="MRIGTDGRVAQARYLASPNCDRRPPGAVPSLLVLHSISLPPGQF
                     GGDAIERLFTNRLDPAGHPYFREIAQLRVSAHFLVRRDGALLQFVPVQLRAWHAGVSR
                     WRGAERCNDNSIGVELEGSDDTRFAAAQYRSLIALARGLAARLPLRACAAHSDVAPGR
                     KTDPGARFDWARFLAALARPQT"
     gene            14740..17589
                     /locus_tag="VFB53_05225"
     CDS             14740..17589
                     /locus_tag="VFB53_05225"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZN86172.1"
                     /translation="MQIARDSAATTPVMGASVADTASAPRSAYPDYKIIRRNGAVVGF
                     EPGKITVAMTKAFLAVNGSQGAASARIRDVVAQLTEQVSGALQRRQPQGGTFHIEDIQ
                     DQVELALMRSGEHDVARAYVLYREERSRARAVEKAARDKPPADSVMHVVDQGARRPLD
                     LGALQRLLESACEGLEGAAAPAQILEATLRDLYDGVPMEEVRKSAILAARAQIEKDPA
                     YSYVTARLLLNTIRLEVLGEEVSQAAMRTRYAAYFPEFIRRGIDAELLDPRLASFDLA
                     RIGRALAAERDLKFGYLGLQTLYDRYFLHVRERRIELPQAFFMRVAMGLALNEKEREA
                     RAIEFYNVLSTFDFMSSTPTLFNAGTQRSQLSSCYLTTVADDLTGIYDAIKENALLAK
                     YAGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLE
                     TWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMENAEWTLFSPSDAPELH
                     DRTGRAFEEAYLRCEDRVARGELKLHKKLPAVQLWRKMLSMLFETGHPWITFKDPCNL
                     RSPQQHVGVVHSSNLCTEITLNTNAEEIAVCNLGSVNLVAHMAEGGLDFDKLKRTVRT
                     AMRMLDNVIDINYYAVDKAKNSNLRHRPVGLGIMGFQDCLHLLRLPYASADAVQFADR
                     SMEMVAYAAYWASTELAEERGRYPSFQGSLWERGVLPQDTLDLLAAERGGYVEVDRSS
                     SLDWEPLRARIRQHGMRNSNCIAIAPTATIANIVGVSASIEPTYQNLYVKSNLSGEFT
                     VTNEYLVSDLKRAGLWDEVMISDLKYFDGNLARVDRAPAGLRRLYATAFEVEPKWLVE
                     AGARRQKWIDQSQSLNIYMAGASGKKLDETYKLAWIRGLKTTYYLRSLGATHAEKSTS
                     KAGQLNAVAMDGGAAGAAAEAQFCAIDNPECEACQ"
     gene            17609..>18076
                     /locus_tag="VFB53_05230"
     CDS             17609..>18076
                     /locus_tag="VFB53_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZN86173.1"
                     /translation="MLSFEEEGKLNAIAGLALQPKSAFAPAAGAAEERFAAGNAFRRI
                     TVEDKRIINSKADVNQLVPFKYKWAWEKYLAACANHWMPQEINMSRDIALWKDPNGLT
                     ADERRIVTRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTH"
BASE COUNT         2609 a         6464 c         6544 g         2459 t
ORIGIN      
        1 tgcggcaggc cccacagcgg gccctgcacg cgcgggaaca ggaggaacag caccagcgcc
       61 gccggcgccg cgtgggccag caggaatccc gcggtgcgca ggttggcgcg catcggccgc
      121 cgcggcgcgc tcaggccggc cagcgtggcg gtcaccgcga gcagcgcggc gcccgccagc
      181 agcgcggtgg ggatgctctg cgagtacagc aggttggtca tcaccaggaa gtagcacagg
      241 aaggcggtca ccgccgcgtc gcggtgcgtg cgcagttcca tcagcttcag gcccgagaac
      301 agcgccagca gcacgatgcc cgaagtgcgg ccgaacagcg tgcggtactc gaggaacacc
      361 gcgccgatcg acagcagcgc gatgccgagc accagccagt gcgacggcag gcgcgcgccg
      421 gcgagggcga tctgcgcgcg ccaggcgtac agggtgagga ccagcagggt gagccaccag
      481 ggcatgcgca gggcgtgcgg cgccaccacc agcagcagcg cgccgaccag ccaggcgagg
      541 tcgcgcaggc cgacgctgcg ctgctccggt gccagggggt gcgcgacgct catgcctcgt
      601 gcagcgccag cgcctgcagg caggcgacgc ggtgcgcgtc gccgctgccg ggcgcgaact
      661 cgagtcccgg caggcgcagg ccgtagcgcg cgcccgcgcg ctcggccgcc agcacccagc
      721 cggcgagccg cgacaggcgc cgctcggcgt cgagcgccgg caggtcgccc cagtccagcc
      781 agagctcgac gcccgccgcg ccgctgaact gcttggtcag catgacgtcg gcgcgcgcca
      841 ccgccttcca cgccacgtgg cgcggcggat cctgcggctg gtagccgcgc aggcccgaga
      901 agtcgtcgtt gccgcgggcg tgcgtgcgcg ccgtgcccgc ggcggcatcc tgcgccggcg
      961 cgggcagcgg cgcgtgctcg gggcgcgggt acaccaggca gcgcaggtcg ggctcgacat
     1021 agctccaggc gcggaacagg cccagcggga agcgcgtctc cagcatcacg cgcgtgagcc
     1081 cgagccagcc gcgccgcgcc gtcggcaccg ccagcacggc ctcctcgatg gcgcccgccg
     1141 gcagctcgag caccacctgc gcgccgctcg cctggtggcg caccagcacg gccgggcggt
     1201 cgtagcgcgc gcggttgtcc aggtacagcc ggaactgcgc cgcctcgccg gcgaacaccg
     1261 gctcggcgcg gcccgcgctc accgccaggc gcgccaggtt gcgcgtggtg tgcaccatcg
     1321 ccgccagccc caggccggcg aggaggaagg tgagggcgaa gcccagctgc agcgcgtaat
     1381 tgatcgagcc gatgagcagg atgaccagcg tcgcgccgaa caaccagccc aggcgcgtcg
     1441 gcacgatgta gacgcggcgg tggccgagca ccacggtgcc gcgctccggc gcggcatagc
     1501 cgtagaacca ttggcgcagg cgggcgccgc cgccgagggc gcgcatcatg tcagctggtg
     1561 gctccgcggt agccccagcg caccttccac gccgccagca ccgcgcgcgg gtaatcgggc
     1621 ttgcccaggc tgccgttgta gcgcccgagg gcgcggaaat agtcgccctt ctccatgtcc
     1681 agatagtgcc gcaggatcag gcagccgtag cgcaggttgg tgcgcaggtg aaacaggttg
     1741 tggtcgcgcg cgccgaccag cttgatccag aacggcatca cctgcatgta gccgcgcgcc
     1801 ccggagcggg acaccgcgta cttgcggaaa ttgctttcca cctcgatcac gcccagcacc
     1861 agctgcgggt cgaggcccgc gcgctgcgcc tcgaagtgga tggtgcgcag cagctccagg
     1921 cgctgcttgc ggtcggcgat gcgcttttcc aggcggcgcg acatctcttc gatccaccgg
     1981 ttgccgtcgc cctgctcctg gaacagcagc tcggggaccg acttgtccgc gatcgcggcc
     2041 gacatgcggc cgcgcaggct ctcggcgagg ggctcgtaga tctgcgcgcc cgcctgcgcg
     2101 gcgagcgggg aggcgagggc ggcggcgagc gcagtggcga gggcgccggc gcgcgtcatg
     2161 cggcggcgag gcgttccttg agaaacgcgg cgagctgcgc gagcggcacg ccctgcggct
     2221 gcgcggcgcg gcgcgacttg tactccgcct cgccggcggc gaggccgcgc tccgacagca
     2281 ccacgcggtg cgggatgccg atcaggtcca tgtcggcgaa cagcacgccg ggacgctcgt
     2341 cgcggtcgtc gagcagcacc tcgatgcccg ccgcctgcag ctcggcgtac agccggtcgg
     2401 cggcggcgcg caccgtctcg ttcttggcgt agccgatggg caccaggcag gcgccgaacg
     2461 gggcgagcgg cgccggcagc aggatgccgc gctcgtcgtg cgcctgctcg atcgccgcgg
     2521 ccaccacgcg cgtcacgccg atgccgtagc agcccatctc catcgggtgc gcggcgccct
     2581 tgtcgtcgag cacggtggcc tgcatggccg cggaatactt ggtgcgcagc tggaagatgt
     2641 ggcccacctc gatgccgcgc tggatcttga gccggccctt gccgtcggga ctgggatcgc
     2701 cgtccatcac gcttctgatg tcggcgacga gcgaaggttc cggcaggtcg cggccccagt
     2761 tcacgccggc caggtggaag ccctccgcgt tcgcgccgca gacgaagtcg ctcatcgcgg
     2821 cgacagtgcg gtcggcgacg atgcgcgccg ccgtggcgac cgccgggcca ctgccagcca
     2881 gaggcggtcc aatgtagccg gccgggccgc ccagcgcctg gcggatctcc gcctcgctgg
     2941 cgaagcggaa cgcgccgatc gccttctgcg ccttgacctc gttcagcgcg tgatcgccgc
     3001 gcaggagcag caggtggaac tgcgcctcgt gcaccagcgc gatcgccttc accgtgcgct
     3061 cgagcggcag cttgagcagc gccgcgacct ccgcgcagct ggtcttgccc ggcgtggcga
     3121 ccttgcgcat cgcctcgccg ggcgcgcggc gcggcgcggc cggcgcgagc gcctcggcca
     3181 gctcgacgtt ggcggcgtag ccggactccg cgctgtaggc gatggcgtcc tcgcccgagt
     3241 cggcgagcac ctggaactcg tgcgagccgg tgccgccgat ggcgccggtg tcggccgcca
     3301 ccgcgcggaa ctgcagcccc aggcgggtga agatgcgcgt gtaggtgtcg tacatgctgc
     3361 ggtactcgcg ctccaggccc gcgtagtcgg cgtggaacga gtagccgtcc ttcatcacga
     3421 actcgcgccc gcgcatcacg ccgaagcgcg ggcggcgctc gtcgcggaac ttggtctgga
     3481 tctggtagaa gtgcaccggc agctggcggt agctcttcag ctcggcgcgc gccaggtcgg
     3541 tgatcacctc ctcggaagtg ggctggatga cgaaatcgcg ctcgtggcgg tccttgaagc
     3601 gcagcagctc gggcccgtag gcctgccagc ggcccgattc gcgccacagc tcgcccggct
     3661 gcaccgccgg catcgacagc tcgatggcgc cggcgcggtt catctcctcg cggatcaccg
     3721 cctccacctt gcgcagcacg cgcaggccga gcggcagcca ggtgtagatg ccggccgcca
     3781 ggcgccggat gtagccggcg cggagcatca gccggtggct ggcgagctcc gcgtcggcgg
     3841 gcgcctcctt gatggtgccg aggtggaatc gggatgtgcg catcgctcgg gcgccgcgtt
     3901 gtgtttgcgc ctcgcctgtg gaaaaatgcg gaggaattct aacttaggtg gtgtggccat
     3961 gctcgaccga gacggctatc gtccgaatgt cggcatcatc ctcgccaacg gccgcaacga
     4021 ggtgttctgg ggcaagcgcg ccaaccagca cgcgtggcag tttccccagg gcggcatcaa
     4081 gcacggcgag tcgccggagc aggcgatgta ccgcgagctc gaggaggaga ccggcctcaa
     4141 ggcggaccac gtgcgcgtcc tcggccgcac gcgccaatgg ctgcgctacg aggtgccgga
     4201 gaagtgggtg cggcgcgact ggcgcggcac ctaccgcggg cagaagcaga tctggtacct
     4261 gctgcgcatg gtcgggcgcg actgcgacgt caagctgcgc accagcgaca agcccgagtt
     4321 cgacgcctgg cgctggcacg agtactgggt gccgctcgag gcggtgatcg aattcaagcg
     4381 cgaggtctac cgcgaggcgc tcgccgagct gcaccactac ctgggggtcg accggggcgg
     4441 tcgcggcgcg cgggcgtcca cgcgctgagc gcgcgcgagc gcggggaggg cggtcatgga
     4501 caggcagtac cgcgaattgc cgatccgcga gctgggcccg gacgccggct tccggcgccc
     4561 gcagcagccg ctgccggcgc gcgtgaccct cgagtcgagc gcgctggagg tgatgaccga
     4621 cctgagccgc gtcagcccgg ccaccatccg gccgcaggcg ccgctggagg gcgccaacca
     4681 gttcatgatt tcgcgcggcg tgcgcctgct gctggtgatc gacgtgcgcg acaccgtgct
     4741 cggcgtgctc acggcgaccg acgtgctggg cgagaaggcg atgcgcgtgt cggtcgagcg
     4801 cggcgcgaag cgcagcgagc tcaccgtcgc cgacctgatg acccccgccg agcgggtgga
     4861 agtgatcgcg ctggaggacc tgcagggggc gcgcgtcggc cacctgctcg agacgctgcg
     4921 ccgctcgggc cggcagcacg cgctggtggt ggaccacgac ggcgccgcgc cggccgcacc
     4981 cccggtgcgg cgcgcgatgg tgcgcggcat cgtctcgctg tcgcagctgg cgcgccagct
     5041 cggcctgctg ctgccgcagg cgggcgaggt ggcgcgcacc ttcgccgaga tcgaggccgc
     5101 gctcggccgc tgagggcgcc gcaggatagc gcctgtcaat tgctcgaatt ggcaaattcg
     5161 atttgattta tcgggggcgg cggcgtagct tggccgcatc ccattccacg cgcagcgcaa
     5221 ggagacggag catgaaacca ctcaaaggca ccaagaccca ccagcacctg aaggacgcct
     5281 tcgccggcga gtcgcaggcc aaccgccgct acctgtattt cgccgccaag gccgacgtcg
     5341 agggccagaa cgacgtgtcg gcgctgttcc gctcgacggc ggaaggcgag accggccacg
     5401 cgcacggcca cctggagtac ctggagcagg tgggcgatcc ggcgaccaac ctgccgatcg
     5461 gccgcacgcg cgacaacctc aagtcggcgg tcgccggcga gacgcacgag tacaccgaca
     5521 tgtaccccgg catggccaag gacgcgcgcg gcgagggctt cgccgagatc gccgactggt
     5581 tcgagacgct ggccaaggcc gagcgcagcc acgccaaccg cttccagaag gccctggacg
     5641 ccctggccga ctgacgatgg cgcgcatcga gcgcggcagc ctgctgtcgc tcgaggccta
     5701 cgcgcgcgaa cgccatgcgt tccgcgcgcg ggtcatcgag cacaagcggc gccgcaccgt
     5761 ccacgtcggc gagcacgtca cgctgatctt cgaggacgaa ctgacgatcc gctaccaggt
     5821 gcaggagatg ctgcgcatcg agcgcatcta cgaggaggac ggcatccggc acgagctgga
     5881 ggcctacaac cccctggtgc ccgacggcgg caacctgaag gccaccatga tgctcgagta
     5941 tcccgaggtc gcggagcgcc agcgcgccct ggcgcgcctg aagggcgtcg aggaccgcgt
     6001 gtggctgcag gtggcgggcg gcgcgcgcgt gtacgccatc gccgacgagg acctggagcg
     6061 cgagaacgcc gagaagacct cggcggtgca tttcctgcgc ttcgagctgg gcgcggagga
     6121 gcgccaggcg ctgcgccgcg gcgccggcct ggcgatcggc atcgaccacc cgcagtacca
     6181 ggccagcgtc gacgcgtcgt cgccagtgcg cgacgcgctc gccgccgatc tcgcctgaac
     6241 cgcggcgcgc gcggcctggc ggcgctgctg ctcggcgcct gcgcggcggc cgccgcgcag
     6301 cccatgtccg actgggagcg ccggcagcag ggccgcgagc agcccgaggc ggcagtcacg
     6361 ctgcccgcgt tcccgcggcc ctcggcgctg ctgccgttct tcgtcagcgc ggcgagcgat
     6421 ttccggtttt tcgtcgacgg cgcctcgctg tcggtgggcg gggacggcgt ggtgcgctac
     6481 accctggtgg cgcgcagccc gcagggcgcc gagaccgtgg tgtacgaagg catgcgctgc
     6541 gcgacgcgcg aggtgcgcgg ttacgccgcc gggcgcgcgg acggcagctg gtcgcggcgc
     6601 gacacgccct ggcgcgccat cggggatccc agcgtgcagc gctggcaccg ggcgctggcg
     6661 accgagtacc tgtgcccggg gaaggtgccg atccaggacg cggccgaagg cgtcgatgca
     6721 ttgcgccgcg gcgggcaccc gcgggcggtc caccgggcgg gaggctagac gaggaccagg
     6781 ttgtcgcggt ggatcagctc gggcccgtcg acatagccca ggacggactc gatctcgctg
     6841 gagggccggc gcaggatcag gcgcgattcc gccgccgagt agttcgcgag gccgcgcgcg
     6901 atctcgcgcc cttccgggcc gaggcaggcc accaccgcgc cgcgctcgaa gtcgccctgc
     6961 accgcgctga cgccgatcgg cagcaggctc ttgcccccct gcagcagcgc gcgcgccgcg
     7021 cccgcatcca gggcgagcgt gccgccgacc gtgacctggt cggcgagcca ctgcttgcgc
     7081 gcggcgagcg gggtggtctg cgacagcaac aggctgccga tgcgttcgcc ccggcccagc
     7141 cgcaccagca cgtcgggctc gcggcccgag gcgatcacgg tgtgcgcgcc gctgcgcgcc
     7201 gcgcgcttgg ccgccagcac cttggtgatc atgccgccgc gcgcgagcgc cgagccggcg
     7261 ccgccggcca tcgcctccag gcgcgcttca cccgcggtcg cttcctcgat gagcctggcg
     7321 cccggcacga ggcgcgggtc ggcgtcgtgc agcccggcct ggtcggtgag gatcaccagc
     7381 gcatcggcct cgaccaggct ggtgaccagc gcggccaggg tgtcgttgtc gccgaccttg
     7441 atctcgtcgg tgaccaccgt gtcgttctcg ttgatcaccg ggatcacgcc gagctcgagc
     7501 agggtcagca gggtggagcg ggcattgagg tagcgctgcc ggtccgccat gtcggcgtgg
     7561 gtgagcagca cctgcgccgt ggcgaggccg tgcttgcgaa agcaggtctc gtaggcctgc
     7621 accaggccca tctgtcccac cgccgcggcc gcctgcagct cgtgcagcgc atgcgggcgc
     7681 ctggtccagc ccaggcgctg caggccctcg gcgatggcgc cggaggacac cagcacgcag
     7741 cgccgcccga gcgcgcgcag ctgcgcgatc tgcgccgccc agcgggcgat cgccgccacg
     7801 tccagcccgc gcccctcgtt ggtgaccagc gcgctgccca ccttgaccac cagggtgcgc
     7861 gcgctggcga gctggccggg catcggcgtc aggcggcgcg ccgcgcgcgc gccttgtcct
     7921 ggcgcgcgcc gcccgcgagg aagcggtagg ccgcgctgca cagcgcgtcg cagcccttgc
     7981 cgccgatggc gctgatcgcg aaccagggac ccttgtagcg cagcttgcgc agcaggcgcg
     8041 cgatgcgcgc gtcggcgtcc gcggcgaggt cgatcttgtt gaacaccagc cagcgcggct
     8101 tgcgatgcag cgccgggtcg tacttcttca gttccagcgc cagcgcgcgc gcatcgcgcg
     8161 ccgggtcggc gtccgcggcg agcggcgcga tgtccaccag gtgcagcagc aggcgcgtgc
     8221 gcgacaggtg gcgcaggaac tggtggccga ggccggcgcc ctcggccgcg ccctcgatca
     8281 gcccgggaat gtcggcgatc acgaagctcg ccccggcggc gacgcgcacc acgccgagcg
     8341 agggattgag cgtggtgaag gggtagtccg ccaccttggg ccgcgcgctg gacaccgcgc
     8401 gcaccagggt ggacttgccg gcgttcggca gcccgagcag gccgacgtcg gcgagcacgc
     8461 gcagctcgag cgcgagcttg cggctctccc ccggcaggcc gcgcgtgaac tggcgcggcg
     8521 cgcggttggt gctggacttg aagtgcaggt tgcccaggcc gccctggccg cccctggcga
     8581 gctgcgcgcg ctcgccgtcg cgcgccaggt cggcgatcag ctcgccgttc ccggcgtcgc
     8641 gcaccagcgt gccgaccggc acgcgcagct ccacgtcgcg cccgccgcgg ccgtactggt
     8701 ccgagccgtg cccggcgtcg ccgtgctcgg cgcggtggat gcgcgcgaac cggtaatcga
     8761 tgagggtgtt gatgttgcgg tcggccaccg cccacacgct gccgccgcgg ccgccgtcgc
     8821 cgccgctcgg gccgccgcgc ggcacgtagc gttcgcgccg gaacgcggcg ctgccgtcgc
     8881 cgcccttgcc ggcgtgcacc tcgatgttcg cttcgtcgat gaatttcatg gcgctgaaaa
     8941 ctaaaaagcc ctaccgcgaa cgatagggct cttcacggtg gttgggcggg cgctcaggcc
     9001 ggcacgatgc tcacggtctt cttgctgtgc gcgccgtgca ccgcgaactg caccttgccc
     9061 gcggtgcgcg cgaacagcgt gtggtcgcgc cccatgccga cgttgcgccc cgggtgcacc
     9121 acggtgccgc gctggcgcac caggatctgg ccggcgttca cctgctcgcc gccgtaggtc
     9181 ttcacgccca gccgcttgga ctgcgagtcg cggccgttcc tggaactgcc gcctgccttc
     9241 ttgtgtgcca tgtgcgagcc ccctgctcag gcgccggcga cgatgtcgtc gatccggact
     9301 tcggtgtagt tctggcgatg cccctgcgac ttctggtagt gcttgcgccg gcgcagcttg
     9361 aagatgcgca ccttgtcgcc gcggccgtgc gcgagcaccg tggccttgac ctgggcgccg
     9421 gcgaccagcg gcgcgcccac gcgcaccgcg tcgcccgcgc cgaccatcag cacctggtcg
     9481 aaggcgaccg ccgcgccgac ttccgcggcg agcgattcga cgcgcagctg ctcgccggac
     9541 tggacgcggt attgcttgcc gccggacttg atgattgcgt acatggggaa cggttgccag
     9601 agtttcgtaa agacttggaa ttatagaaga atccggctgc ctgcgtcaaa gacttgtatg
     9661 ctatggttcg cgcccccgtc gccgcgccat gcccgtcacc gagaaatccg ccagcgccat
     9721 cctgtccccg gtcgccgagg acatgcggca ggtggacgcc ctgatccgcg agcgcctgga
     9781 ttccgacgtg gccctggtgc gccaggtggc cgaatacatc gtggcgggcg gcggcaagcg
     9841 gctgcgtccg gcgctgctcc tcctcgcggc gggggcggtg ggcttccgcg gcgcggcgcg
     9901 cctggaactc gccgcggtgg tggagttcat ccacaccgcc acgctgctgc acgacgacgt
     9961 ggtcgacgag tcgcagctgc ggcgcgggcg gcgcaccgcc aacgcggcct acggcaatgc
    10021 ggcctcggtg ctggtgggcg acttcctgta ctcgcgcgcc ttccagatga tggtcgggct
    10081 gcagagcatg cgcgtgatgc gcgtgctggc cgacgccacc aacaccatcg ccgaaggcga
    10141 ggtgctgcag ctgatgaact gccacgaccc ggaggtgggc gaggagcgct acctcgacgt
    10201 ggtccggcgc aagacggcga agctgttcga ggcggcggcg cgcctcggcg gcatcctcgg
    10261 cggcgccgcg cccgacctgg aggaaggcct ggcggcctac ggcatgcacc tcggcaccgc
    10321 cttccaggtg gtcgacgacg tgctcgacta ctcgggcgcc gcgcaggaga ccggcaagag
    10381 cctcggcgac gacctcgccg agggcaagcc gacgctgccg ctgatccggg cgatgcaggc
    10441 cggcagcccc gccgaacgcg cgctggtgcg gcgcgcgatc atcgagggcg ggcgggcgga
    10501 tttcgcgccg gtgctggcgg cgatccgcag caccggcgcg ctggactacg cgcgcgccgc
    10561 ggcgcagcgc gaagccgcgg tggccgcgca ggcgatcgcc ggcctgccgg cctcggcgca
    10621 cagggattct ttgctagaat tggcgtcctt ttcggtgacg cgccgcagct agtcccggcg
    10681 cgcttcgggg tgtagctcag cctggtagag tactgcgttc gggacgcagg tgtcgctggt
    10741 tcgaatccag tcaccccgac cattcccccg ctcccgcggc cgcggagttt cccggagttt
    10801 ccgagagttt cctagaatgg gcccatgcgc ctgatcctgc tggccctcgc cgtgctggcc
    10861 ctcgcctggc tggtgcgccg cgcgctgcgc gcgcggcgcg cggacgcggc gcccccgggc
    10921 ggcgcccccg gcgaactggt ggcctgcgcg cattgcggcg tgaacctgcc gcgcgccgag
    10981 gcgcgcgccg cggcgcaggg cctgtattgc agcgaggagc acgcgcggct cgggccgcgg
    11041 cggccgtgat ggcgacgctc gcgtacgcgc cgctgttcgc gcgcgaggac cggccgcccg
    11101 acgcgttctg gatctcgctg cagtacttca atctgtaccg catcgtggtg gcgctgctgt
    11161 tcctggtggc cacctacttc acctacgagc cgatcaactt cggcgcccac gacctgcgcc
    11221 tgttccgcgc cggctgcgtc ggctacctgg cgctgggcat cgccctgcag atcgtgctgc
    11281 gctcggtgcg cgagcgcttc aacctgcagc tctcggcgca cgcgctgctc gacgtgatcg
    11341 ccatcccgct gctgatgtac gccagcggcg gcatgcgcag cggcctgggg gtgatgctgc
    11401 tcatctcgat caccggcgcc ggcctggtgg cgccgctgcg cctgaacttc ctgtatgccg
    11461 cgctcgccac catcgccgtg ctgctggagg aggcctactg ggtgctggcg ctcgacctgc
    11521 ccgcggcgag cttcgtgccg ccggcgctgc tgtcgatggg ctatttcgcc actgccggcg
    11581 ccaccggctg gctggcgcag cgcctggccg ccaacgagcg cctggcgcgc gagcgcggcc
    11641 gcgcgctcgc caaccagacg cgcgtcaacc agctggtgat cgccgacatg caggacgggg
    11701 tgctggtgct ggaccgccag ggccgcgtgg tcgagcacaa cccgcaggcc atgcggctgc
    11761 tggggatgga cgcgctgcgc ggcctggcgc tgggcgcggt gctgcccggg ctggaggcgc
    11821 gctggctggc gtggcgcgac gccggcgcgc gcgccgcggt ggccatcgac ctggaggcgc
    11881 gcggcaagga ggtgcgcgcg cggctgctcg ataccggcac cgaggaccgc ttcacggtgg
    11941 tgttcgtcga ggacatgacg cgctcgcgcg agcacgcgca gcagttgaag ctcgccgccc
    12001 tcggccggct caccgccaac atcgcgcacg agatccgcaa cccgctgtcg gccatcagcc
    12061 acgcctccga gctgctcgac gaggagcgcc gcgacgagga ccgcgcgcgc ctcacgcgca
    12121 tcatccacga caacacgcgg cgcctggagc gcctggtggc cgacgtgctg cagctcaacc
    12181 ggcgcgaccg catcgccgcc gagcgcatcg cgctcggcgc ctggctgccc gccttcctcg
    12241 aggagttcgg cgccaacgaa gcggtgcccg ccgggcgcat cgccatcgag ccgggacgcg
    12301 gcgtgtggat ggagttcgac cgcgagcacc tgcgccaggt gctgtggaac ctgctgcgca
    12361 acgcggtgcg ccacgcgcgc gccgagccgg gcagcgtgcg cctcgcggca cgcgccttcg
    12421 gcgaccaagt agaattgaac gtcaccgaca acggccccgg ggtgcccgag tccgtgcagg
    12481 gacagctgtt cgagcccttc ttcaccaccg acagcaaggg aaccggcctg ggcctgtacc
    12541 tcgcacgcga gttgagcgcc gccaaccggg cactgctcga gtacgtggac gaccgcgccg
    12601 gcgcgcattt ccgcatcgtg tgccgcggcg gggcgctctg atgcggcgcg ccgaccgcaa
    12661 gcccgcgcag cgcgagcgcg tggtgctggt ggtggacgac gagcccgaca tccgcgaact
    12721 gctcgagctg acgctcgcgc gcatgggcct gggcgtggtg gcggtgggct cgatcgccga
    12781 ggcgcgcgag cagctcagga ccgggcgctt cgagctgtgc ctgaccgaca tgcggctgac
    12841 cgacggcgag gggctggacc tggtgcgcca catcggcagc ctcgacaacg acctgccggt
    12901 ggcggtgatc accgcgcacg gcagcgccga gaacgcggtg gcggcgctca aggcgggcgc
    12961 cttcgactac gtgtccaagc ccgtgggcct ggagcagctg cgcgccctgg tcaagtcggc
    13021 cctgtcgctg ccggaccagg gggacgagcc gccgcgcgac ctgccgctgc tcggcgcgag
    13081 cgcgccgctg gcgcaggtgc gcgagctgat cggcaagctg gcgcgcaccc aggcgccggt
    13141 gtacgtctgc ggcgagtcgg gcagcggcaa ggagctcgcg gcgcgcctga tccacggcca
    13201 gggcgcgcgg cgcgagcggc cgttcgtgcc ggtgaactgc ggcgcgatcc cggaggcgct
    13261 gatggagagc gagttcttcg gctacaagaa gggcgccttc accggggcgg aacaggaccg
    13321 cgacggcttc ttccaggccg ccgacggcgg cacgctgttc ctcgacgagg tggcggacct
    13381 gccgctggcg atgcaggtga agctgctgcg cgcgatccag gaaaagcgcg tgcgcaaggt
    13441 cggcgccacg caggaggatc cggtcgacgt gcgcatcatc agcgccacgc accaggatct
    13501 ggcgcggctg gtggaggcgg ccaggttccg ccaggacctg ttctaccgcc tgaacgtgat
    13561 cgagctgaag atgccgccgc tgcgcgagtg ccgcgaggac atcgcgacca tcgcgcgcag
    13621 catcctggag cgcatcgcgc gccaggccgg cgcggtcgcg gcgcgcctgt cggccgcggc
    13681 gctcgcggag ctcgccgcct acgacttccc gggcaacatc cgcgagctgg agaacatcct
    13741 ggagcgcgcc acggcgctgt ccgcgggcgg cgagatcggc cccgaagacc tgcggctcaa
    13801 gcccgcggcg gccgaggacg cgggggcgcg cgccgcggag ggcgcgctcg gcgcgccgct
    13861 gccggagtac ctggaccgcg tcgagcgcga agcgattctg caggcgctcg cgcgcaccaa
    13921 tttcaaccgc accgccgccg ccaagctgct cggggtcacc ttccgcacgc tgcgctaccg
    13981 catgcagcgc ctcggcatca aggatgagaa tcggcactga cgggcgggtg gcgcaggcgc
    14041 gctacctggc ctcgcccaat tgcgaccggc gcccgcccgg cgccgtgccg tccctgctgg
    14101 tgctgcattc gatcagcctg ccgccggggc agttcggcgg cgacgcgatc gagcggctgt
    14161 tcaccaaccg gctcgaccct gccgggcacc cgtacttccg tgaaatcgcg cagctgcggg
    14221 tgtcggcgca cttcctggtg cggcgcgacg gcgccctgct gcagttcgtg ccggtgcagc
    14281 tgcgcgcctg gcacgccggg gtgtcgcgct ggcgcggcgc cgagcgctgc aacgacaact
    14341 cgatcggcgt cgagctcgag ggcagcgacg acacgcgctt cgccgcggcc cagtaccgca
    14401 gcctgatcgc gctggcgcgc ggcctcgcgg cgcggctgcc gctgcgcgcc tgcgcggcgc
    14461 acagcgacgt ggcgcccggg cgcaagaccg accccggcgc gcgcttcgac tgggcgcgct
    14521 tcctcgccgc gctcgcccgc ccccagactt agtagtccgg gagctccggc tccccatccc
    14581 tagtagcttc gctgcactgc gccattcgcg cggcgcttgc aaaataaaac cgccgtggta
    14641 cattgccccc actagaacta gttagcaatc agtggttcag cactatatat tgtggtttta
    14701 gtgcagatcg ccgaccgggc ggccagggga gacgcaaaca tgcagatcgc gcgagacagt
    14761 gccgcaacga cccccgtcat gggtgcgagc gttgccgaca ccgccagcgc gccgcgcagc
    14821 gcatatccgg actacaagat catccgccgc aacggcgcgg tggtgggctt cgagccgggc
    14881 aagatcacgg tggcgatgac caaggccttc ctggcggtga acggcagcca gggcgcggcc
    14941 tcggcgcgca tccgcgacgt ggtggcgcag ctcaccgagc aggtgtccgg ggcgctgcag
    15001 cgccgccagc cgcagggcgg caccttccac atcgaggaca tccaggacca ggtcgagctc
    15061 gcgctgatgc gctcgggcga gcacgacgtg gcgcgcgcct acgtgctgta ccgcgaggag
    15121 cgctcgcgcg cgcgcgccgt ggagaaggcg gcgcgcgaca agccgccggc ggacagcgtg
    15181 atgcacgtgg tcgaccaggg cgcgcgccgc ccgctcgacc tcggcgccct gcagcgcctg
    15241 ctggaatcgg cctgcgaggg cctggagggc gcggccgcgc cggcgcagat cctggaagcg
    15301 acgctgcgcg acctgtacga cggcgtgccg atggaggagg tgcgcaagtc ggcgatcctc
    15361 gccgcgcgcg cgcagatcga gaaggacccg gcctacagct acgtcacggc gcgcctgctg
    15421 ctcaacacca tccggctgga ggtgctgggc gaggaggtca gccaggcggc gatgcgcacg
    15481 cgctacgccg cctacttccc cgagttcatc cgccgcggca tcgacgccga gctgctcgac
    15541 ccgcggctgg cgagcttcga cctggcgcgc atcggccgcg cgctcgcggc cgagcgcgac
    15601 ctgaagttcg gctacctcgg cctgcagacc ctgtacgacc gctatttcct gcacgtgcgc
    15661 gagcgccgga tcgagctgcc gcaggccttc ttcatgcgcg tggcgatggg cctggcgctg
    15721 aacgagaagg agcgcgaggc gcgcgccatc gagttctaca acgtgctgtc gaccttcgac
    15781 ttcatgagct cgaccccgac gctgttcaac gccggcacgc agcgctcgca gctgtcctcc
    15841 tgctacctga cgacggtggc cgacgacctc accggcatct acgacgccat caaggagaac
    15901 gcgctgctcg ccaagtacgc cgggggcctg ggcaacgact ggacgccggt gcgcgcgctc
    15961 ggctcgcaca tcaagggcac caacggcaag tcgcagggcg tggtgccgtt cctcaaggtg
    16021 gtgaacgaca ccgccgtggc ggtgaaccag ggcggcaaga ggaagggcgc ggtgtgctcc
    16081 tacctggaga cctggcacct ggacatcgag gagttcctcg agctgcgcaa gaacacgggc
    16141 gacgaccggc gccgcacgca cgacatgaac acggcgaact ggatcccgga cctgttcatg
    16201 aagcgcgtga tggagaacgc cgagtggacg ctgttcagcc cctccgacgc ccccgagctg
    16261 cacgaccgca ccgggcgcgc cttcgaggag gcgtacctgc gctgcgagga ccgggtggcg
    16321 cgcggcgagc tgaagctgca caagaagctt ccggcggtgc agctgtggcg caagatgctg
    16381 tcgatgctgt tcgagaccgg gcatccgtgg atcaccttca aggatccctg caacctgcgc
    16441 agcccgcagc agcacgtcgg cgtggtgcac agctccaacc tgtgcaccga gatcaccctc
    16501 aacaccaacg ccgaggagat cgcggtgtgc aacctgggtt cggtgaacct ggtggcgcac
    16561 atggccgagg gcgggctgga tttcgacaag ctgaagcgca ccgtgcgcac ggcgatgcgc
    16621 atgctcgaca acgtcatcga catcaactac tacgcggtgg acaaggcgaa gaactcgaac
    16681 ctgcgccacc gcccggtggg gctgggcatc atgggcttcc aggactgcct gcacctgctg
    16741 cgcctgccct acgcctcggc cgacgcggtg cagttcgccg accgctcgat ggagatggtg
    16801 gcctacgccg cgtactgggc ctccaccgag ctggccgagg agcgcgggcg ctacccctcc
    16861 ttccagggct cgctgtggga gcgcggcgtc ctgccgcagg acacgctcga cctgctggcc
    16921 gccgagcgcg gcggctacgt ggaagtggac cgctcatcga gccttgactg ggagccgctg
    16981 cgcgcgcgca tccggcagca cggcatgcgc aactcgaact gcatcgccat cgcgcccacc
    17041 gcgaccatcg ccaacatcgt cggcgtgtcg gcctcgatcg agccgaccta ccagaacctg
    17101 tacgtcaaat ccaacctgtc gggcgaattc accgtcacca acgagtacct ggtgtcggac
    17161 ctgaagcgcg ccggcctgtg ggacgaagtg atgatctccg acctgaagta cttcgacggc
    17221 aacctcgcgc gcgtcgaccg cgcgcccgcc gggctgcggc gcctgtacgc caccgccttc
    17281 gaggtcgagc cgaaatggct ggtggaggcc ggggcgcggc gccagaagtg gatcgaccag
    17341 tcgcagtcgc tcaacatcta catggccggc gcctcgggca agaagctcga cgagacctac
    17401 aagctcgcct ggatccgcgg cctgaagacc acctattacc tgcggtccct gggcgccacg
    17461 cacgccgaga aatccacctc caaggccggg cagctgaacg ccgtggcgat ggacggcggc
    17521 gcggccggcg ccgccgcgga agcgcagttc tgcgccatcg acaatcccga atgcgaagct
    17581 tgccaatgac catcgaggcc tgccactgat gctcagcttc gaggaagagg gcaagctgaa
    17641 cgccatcgcc ggcctggcgc tgcagccgaa gagcgccttc gcgccggccg ccggcgccgc
    17701 cgaggagcgc ttcgccgccg gcaacgcctt ccgccgcatc acggtggagg acaagcgcat
    17761 catcaactcc aaggccgacg tcaaccagct ggtgccgttc aagtacaagt gggcctggga
    17821 gaagtacctg gccgcctgcg ccaaccactg gatgccgcag gaaatcaaca tgagccgcga
    17881 catcgccctg tggaaggatc ccaacgggct caccgcggac gagcggcgca tcgtcacccg
    17941 caacctcggc ttcttcgtca ccgcggattc gctggcggcc aacaacatcg tgctcggcac
    18001 ctaccgccac atcaccgccc cggagtgccg ccagtacctg ctgcgccagg ccttcgagga
    18061 ggcgatccac acccac
//
LOCUS       DASRIE010000118        12276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_126263, whole genome shotgun sequence.
ACCESSION   DASRIE010000118 DASRIE010000000
VERSION     DASRIE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126263"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFB53_05235"
     CDS             <1..521
                     /locus_tag="VFB53_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86174.1"
                     /translation="ETILQIEFTDSARFTLGPNAEFEVSRYSFAAGGPGEESFQSRVI
                     KGAFRFVTGLIARKEKKNMQVGVIVATIGIRGTQVEGEVTERQEKDGKTVDASARVAL
                     LEPEGAPRETAIVVENKFGSVVVDKPGYGTEIPDEKSPPSPVRRMQLRTIDNLQRAIR
                     SPARTGGPARRP"
     gene            complement(691..1686)
                     /gene="egtD"
                     /locus_tag="VFB53_05240"
     CDS             complement(691..1686)
                     /gene="egtD"
                     /locus_tag="VFB53_05240"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZN86175.1"
                     /translation="MARIQRRARSAGLGRTRFTGSLLPADGFREDVIEGLSQPQKALP
                     PKYFYDAAGSRLFERICRLPEYYPTRAELSLTRAHLADIARFAGRGCDLIEYGSGASL
                     KTRLLLRALRPAAYVPVDISHAALAGAARRLAREFGWLHIVAVAGDFSQPLRLPVFRR
                     RARRRRVVYFPGSTIGNLDLEEAQAFLSMSRAQVGAGGAMLIGVDLKKDARLLHAAYN
                     DARGVTARFNLNLLARINRELGADFDLRRFAHYAYYNAPLGRIEMHLVSLARQRVRLA
                     RHRFAFEPGETIHTENSYKYAVGEFRALAAEAGFRTARVWRDARGRFALFGLVAA"
     gene            complement(1647..2513)
                     /locus_tag="VFB53_05245"
     CDS             complement(1647..2513)
                     /locus_tag="VFB53_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN86176.1"
                     /translation="MRSTERRLHSLEKIVAFGALAAVLFVSAGFARADAMPKLRSSVV
                     LVLDAESGETLYEKNSDTVLPIASISKLMTALVVLERKLDLEQRVVISREDFIATRHS
                     RVRARLRAGSVLTRDDLLLLALMASDNLAAAALARTYPGGTEAFVAAMNAEAGRLALH
                     DTRFADPTGLSSDNVSSAQDLARLVKAAHGYPLIREYSTRPSAHVRARGGTVGFNNTN
                     SLVRSGSWDIELSKTGYISAAGRCLVMHMRVASRDLVVILLDSWGKHSRIGDARRIRK
                     WLESSAARGPQG"
     gene            complement(2643..4472)
                     /locus_tag="VFB53_05250"
     CDS             complement(2643..4472)
                     /locus_tag="VFB53_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZN86177.1"
                     /translation="MVNPATHEHGPSRAQPLRGNEWRVLGTLLPYLLEYRWRVLAALA
                     LLTCAKLANVAVPLVMKDVVDGLDPTLAVLAVPAALLAAYGLLRFSTTLFAELRDVVF
                     VRVTQRAIRRVALGVFRHLHALSLRFHLERQTGGMTRDIERGTRGISTLLSYLIFSIL
                     PVILEFGLVAAVLLAKFDWRFAAITFGAVAVYLAFTFLVSEWRIAIRKRANELDSRAN
                     TRAIDSLLNYETVKYFGNEEFEAGRYDDSLRRYEAAATASEASLGLLNIGQSLVIAVA
                     VTALMFLAAEGVATRSLTLGDLVLVNALLIQLYIPLNFLGMVYREIKQSLADMDRMFR
                     LLGENLEVEDAPGAAALAPGPATVEFRQVDFSYEPARRILEQVSFGIPAGQRVAVVGH
                     SGSGKSTLARLLYRFYDVGGGAILVNGRDIRELTQKSLRAAIGIVPQDTVLFNDTILY
                     NIQYGRPGASGAEVIEAARAAHIHELIESLPLKYQSTVGERGLKLSGGEKQRVAIARA
                     ILKDPAILIFDEATSALDSRSEKAIQAELERISRGRTTLVIAHRLSTVMDADQILVLE
                     HGRIVERGTHGELLAAGGEYARMWALQQQAAEAEAVLERAKAA"
     gene            4568..5251
                     /locus_tag="VFB53_05255"
     CDS             4568..5251
                     /locus_tag="VFB53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN86178.1"
                     /translation="MNLRAIKPLKETQTRILDAAEELFMQHGFEGASMRMLTARAGVN
                     LAAVNYHFGSKDALIEAVFRRRLDPMNAERVAELERREQAADGRPLEADAIIRAFLGA
                     SLRMIEDTRGGGRTFTRLLGRAYTEPAKPIRQLIGQMYAPAMERFKAAFVRALPELPK
                     DELVWRMHFMFGTLAYTLAATDTVQLIAGAKPEDRYDARVLEDRLAAFLVAGLLAPLA
                     GSLRVKKAA"
     gene            5356..7743
                     /locus_tag="VFB53_05260"
     CDS             5356..7743
                     /locus_tag="VFB53_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN86179.1"
                     /translation="MGATWAAALGLPAATNAVLFAVFVAAAAACNLPGLRRRLLSDPL
                     LAIYRRILPDMSQTEKEAIDAGTVWWDADLFSGRPDWERLLHTPAPRLNAEEQAFLDG
                     PVEQACAMADDWEITHERYDLPPQLWQYLKDQGFFGMIIPKKYGGLGFSALGHSAVVM
                     KLSSRSNALGVTVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGQEIPCFALTSPEAG
                     SDAASIPDFGVVCKGTWQGRETLGMRLTWDKRYITLGPVATLLGLAFRLYDPDRLLGG
                     KDDLGITCALIPTATPGVNIGRRHLPLNAVFQNGPNSGKDVFVPLEWIIGGRDYAGKG
                     WMMLMNCLAAGRAISLPTSSVGGAKALARVTGAYARVRSQFRTPIGKLEGVEEALGRI
                     AANAYLMEATRVMTAGAVDLGEKPSVLSAIAKYHMTERARDCVNDAMDIHGGKGICLG
                     PNNWVGRGYQVLPVGITVEGANILTRTLIIFGQGAIRCHPYVLREMRAAKELKGEEAS
                     REFDDALTSHIGHTLSNGVRAVVHGLTRAMFAAAPLSAAPETRHYYRHVTRLAAAFAF
                     LADVSMLAMGGALKRKEKISGRLGDVLSMMYLVSATLKHYEDQGRLKADLPMVRWAVR
                     DALYSAQQAIDQVLSNFPVKALATLLRWTIFPLGMPFRPPLDSRNHECAKIALEPGAA
                     RDRLTAGIYLAKGETDATGVLETAFLATVACEPIEKKFRDAVKAGALAVRSGVDTALI
                     ARDKGVISAEEYAQWQKKEALRKRVIMVDDFPQDFGRAEIVQKLQDYQPVKAKAA"
     gene            7745..9037
                     /locus_tag="VFB53_05265"
     CDS             7745..9037
                     /locus_tag="VFB53_05265"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN86180.1"
                     /translation="MNPVYVIDGARTPFLKSKNRPGPFAAGDLAVQAGRTLLSRQKFA
                     PQELDEVILGCAAPSVDEVNIGRVAALRMGCGEKVPGWTVMRNCASGMQALDSGVSNI
                     LAGRSQLVLAGGVDALSRAPLLYNDRMVNWFSDLAAAKTGGQRALLFARLPFKALLAP
                     VIGIMKGLTDPMVGLLMGQTAENLAHRFGITREQMDAYSVRSHRRVARAQDEGPLKAG
                     GGEVEALYDAKGNAYALDDGVRRDATPESLAKLKPFFDRKYGNVTPGNSSQITDGAAW
                     LILASEEAVRRHGLAPLGRIVDTQWAGLDPAQMGLGPVHAATPILKRHGLALDDLDYW
                     EINEAFAAQVLGCLAAWKDEGYCKEQLGLERAMGELDAEKLNVDGGAVALGHPVGASG
                     ARIVLHMLQLLKRKNAKRGIASICIGGGLGGAMLLEAA"
     gene            9040..10980
                     /locus_tag="VFB53_05270"
     CDS             9040..10980
                     /locus_tag="VFB53_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZN86181.1"
                     /translation="MDRAPNATLAKHWRWETDRQGLAWLTFDKQGESTNTFSREALVE
                     LGAALEAIAAARPRGLVIRSAKENFIAGADINQFTRFQTPEEALEFTRLGWDTMQKLR
                     DLPFPTTAMVNGFCMGGGLEMALACTYRVALDDPKTRFAFPEVMLGIWPFWHGLQWLP
                     KLIGPAAALDMILTGRAVDARRAKKTGLATEAVPLRVLENTARQVTLEAKPPPRLGLL
                     ERLMLGIGRRFVAAQARKQVARRARREHYPAPYAILDAWVKYDGDPFINPDDPVTSIK
                     ALSGHPTTRNLIRIFFLQERMKGLAKGVDFKARQVHVIGAGVMGGDIAAVCAMRGITV
                     TLQDTSPERLAPAVKRAAELFAKRLKDPRRVRDALDRLIPDVSGAGAARADVVIEAIF
                     ENLQAKRELFAKLEAVARPDAVLATNTSSLKLADVAAQFADPSRLVGIHFFNPVPQLQ
                     LVEVVSGNATRPEMTGRAIAFVRQIDKLPLPVQDSPGFLVNRVLGPYMQNAFRLLDEG
                     ARPETIDAAMEAFGMPMGPIELADTVGLDICLAAGKELAKKGAEAPQVLLNKVALGQL
                     GRKTGQGIYRYAGGKPVKGQPGAWRPELVEALVEPYLAEAKAVLAEGIVADAELIDAG
                     LIFGTGFAPFRGGPLHYLAARK"
     gene            11057..11392
                     /locus_tag="VFB53_05275"
     CDS             11057..11392
                     /locus_tag="VFB53_05275"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZN86182.1"
                     /translation="MPADANHNGDIFGGWIMAQVDVAGGTVAGRVARGRVATVSVNSF
                     VFKQPVQIGDILSLYADVVRIGNTSITVNVEVFAERGRAESKVVKVTEATLTYVAIDA
                     HGRPRPVPK"
     gene            11389..12240
                     /locus_tag="VFB53_05280"
     CDS             11389..12240
                     /locus_tag="VFB53_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86183.1"
                     /translation="MSGTAVEFAARDGTRLAGTLYRPRAPNRRAVLLQAASGVKQEYY
                     GKFAAYLATRGFAALTFDYRGIGRSRAPGTLRGVGARMRDWAEKDIAGALDHLERATH
                     GARLVGVGHSFGGQAFGLVPGNERYVAALTFGSQSGYWRHWSGRERAGMWFLSHLLLP
                     GVSRALGYFPAQRFGQGEDLPKGVASEWASWCRHPGYLVGRLGAQEAYAQFRAPIRAY
                     VATDDSYAPPQAVEAFLAFYPGSSRKLIPVDPRAAGGEPIGHFGYFRERLRDSLWREA
                     ADWLANA"
BASE COUNT         1882 a         4313 c         4380 g         1701 t
ORIGIN      
        1 ccgagaccat cctgcagatc gagttcaccg acagcgcgcg cttcacgctg gggcccaacg
       61 cggaattcga ggtgtcgcgc tacagcttcg ccgcgggcgg tcccggcgag gagtccttcc
      121 agtccagggt catcaagggc gcgttccgct tcgtcaccgg gctcatcgcg cgcaaggaga
      181 agaagaacat gcaggtcggc gtgatcgtcg ccaccatcgg catccgcggc acgcaggtcg
      241 aaggcgaggt gaccgagcgg caggagaagg acggcaagac ggtggacgcc tccgcccggg
      301 tggcgctgct cgagccggaa ggcgcgccgc gcgagaccgc catcgtcgtg gagaacaagt
      361 tcggcagcgt ggtcgtcgac aagcccggct acggcaccga gatcccggac gagaagagcc
      421 cgccgtcgcc ggtgcggcgc atgcagctgc gcaccatcga caacctgcag cgcgcgatcc
      481 gctcgcccgc gcgcacgggc gggccggcgc gcaggccgta ggcaatcgga gtcggcgcgc
      541 cgaatcacgc ggtgcctgcg tttgcctctc catgatctcg tgttttttgg ccgggtgaat
      601 cgtccggcca aaaaacacga gaccgttttt aaaggcaacg acccgaccct ggcgatcgct
      661 tccgggaaaa tcaggctctg atcccgattt tcaggccgcg acgagcccga acagggcgaa
      721 gcgtccccgg gcgtcccgcc agaccctggc ggtgcggaag cccgcttccg cggccagggc
      781 gcggaattcg cccaccgcgt acttgtacga gttctcggtg tggatggtct cgccgggctc
      841 gaaggcgaag cggtggcgcg cgagccgcac gcgctgccgc gcgagcgaca ccaggtgcat
      901 ctcgatgcgc cccagcggcg cgttgtagta ggcgtagtgc gcgaagcgcc gcaggtcgaa
      961 gtccgcgccg agctcgcggt tgatgcgcgc gaggaggttg agattgaagc gcgcggtcac
     1021 gccgcgcgcg tcgttgtagg ccgcgtgcag cagccgcgcg tccttcttca ggtcgacgcc
     1081 gatcagcatg gcgccgccgg cgcccacctg cgcgcggctc atcgacagga aggcctgcgc
     1141 ctcctcgagg tcgaggttgc cgatggtcga tccggggaag tacaccacgc ggcgccgccg
     1201 cgcgcgccgg cggaacaccg gcaggcgcag cggctgggag aaatcgcccg ccaccgcgac
     1261 gatgtgcagc cagccgaact cgcgcgccag gcgccgcgcc gcgccggcga gcgccgcgtg
     1321 cgagatgtcc accggcacgt aggccgcggg ccgcagcgcg cgcagcagca ggcgcgtctt
     1381 caggctggcg ccgctgccgt actcgatcag gtcgcagccg cgcccggcga agcgcgcgat
     1441 gtcggcgagg tgcgcccgcg tgagggaaag ctcggcgcgc gtcgggtagt actcgggcag
     1501 gcggcagatg cgctcgaaca ggcggctgcc cgcggcgtcg tagaagtact tgggcggcag
     1561 ggccttctgc ggctgcgaca ggccctcgat cacgtcctcg cggaagccgt ccgcgggaag
     1621 gagcgaaccg gtgaagcggg tgcggcctag ccctgcggac cgcgcgcggc gctggattcg
     1681 agccacttgc ggatgcgccg ggcgtcgccg atgcgcgagt gcttgcccca ggaatcgagc
     1741 aggatcacca ccaggtcgcg cgaggcgacg cgcatgtgca tcaccaggca gcggccggcg
     1801 gcggagatgt agccggtctt ggagagctcg atgtcccagc tgcccgagcg caccaggctg
     1861 ttggtgttgt tgaagcccac cgtgccgccg cgcgcgcgca cgtgcgcgct ggggcgcgtg
     1921 gaatattcgc ggatcagggg atagccgtgc gccgccttca ccagccgggc gagatcctgc
     1981 gcgctcgaga cgttgtccga cgacaggccc gtgggatcgg cgaagcgcgt gtcgtgcagc
     2041 gcgaggcggc cggcctcggc gttcatcgcc gcgacgaagg cctcggtgcc gcccgggtag
     2101 gtgcgcgcca gcgccgctgc ggcgaggttg tcggaggcca tcagcgcgag gagcagcagg
     2161 tcgtcgcgcg tcagcaccga gcccgcgcgc aggcgcgcgc gcacgcgcga atggcgcgtg
     2221 gcgatgaagt cctcgcggct gatgaccacg cgctgctcca ggtcgagctt gcgctcgagc
     2281 accaccagcg ccgtcatcag cttggagatc gaggcgatcg gcagcacggt gtcgctgttc
     2341 ttctcgtaca gcgtctcgcc gctctcggcg tcgagcacca gcacgaccga cgagcgcagc
     2401 ttcggcatgg cgtcggcgcg cgcgaagccc gcgctgacga acagcaccgc ggcgagcgcg
     2461 ccgaacgcga cgattttttc gagcgaatga agcctgcgtt ccgtgctgcg catttcccgc
     2521 cgccgatcca aggtcgggcg agtttagcag gccagattaa aaatgcaaat gaaaataagg
     2581 gggtagatga aaataactag aaaggaagtt aactagccgg acaaattccc gcccccgggg
     2641 gcctaagccg ccttggcgcg ctccaggacc gcctcggcct cggcagcctg ctgctgcagc
     2701 gcccacatgc gcgcgtattc gcccccggcg gcgagcagct cgccgtgggt gccgcgctcg
     2761 acgatgcggc cgtgctcgag caccaggatc tggtcggcgt ccatcaccgt ggacaggcgg
     2821 tgcgcgatca ccagcgtggt gcggccgcgc gagatgcgct ccagctccgc ctggatcgcc
     2881 ttctcggacc tcgaatcgag cgccgaggtg gcctcgtcga agatcaggat cgcggggtcc
     2941 ttgaggatgg cgcgtgcgat cgccacgcgc tgcttctcgc ccccggagag cttcaggccg
     3001 cgctcgccga cggtgctctg gtacttcagc ggcaggctct cgatgagctc gtggatgtgc
     3061 gccgcgcgcg ccgcctcgat cacctcggcg cccgaggccc cggggcgccc gtactggatg
     3121 ttgtagagga tggtgtcgtt gaacagcacc gtgtcctgcg gcacgatgcc gatggccgcg
     3181 cgcaggctct tctgcgtcag ctcgcggatg tcgcggccgt tcaccaggat cgcgccgccg
     3241 cccacgtcgt agaagcggta cagcaggcgc gccagcgtcg acttgcccga gcccgagtgc
     3301 ccgaccaccg cgacgcgctg gcccgccggg atgccgaagg acacctgctc gaggatcctg
     3361 cgcgccggct cgtaggagaa atccacctgg cggaactcca ccgtcgccgg ccccggcgcc
     3421 agcgccgcgg cgccgggcgc gtcctcgacc tcgaggttct cgcccagcag ccggaacatg
     3481 cggtccatgt cggcgagcga ctgcttgatc tcgcggtaca ccatgccgag gaagttgagc
     3541 gggatgtaga gctggatcag gagggcgttc accagcacca ggtcgccgag ggtgaggctg
     3601 cgcgtggcca cgccctcggc ggcgaggaac atcagcgcgg tcaccgccac cgcgatcacc
     3661 aggctctggc cgatgttgag caggccgagc gaggcctcgc tggcggtggc ggcggcctcg
     3721 tagcggcgca ggctgtcgtc gtagcgcccg gcctcgaact cctcgttgcc gaagtacttc
     3781 accgtctcgt agttgagcag gctgtcgatg gcgcgggtat tggcgcgcga gtcgagctcg
     3841 ttggcgcgct tgcggatggc gatgcgccac tccgacacca ggaaggtgaa cgccaggtac
     3901 accgccaccg cgccgaaggt gatggcggcg aagcgccagt cgaatttcgc cagcagcacg
     3961 gccgccacca ggccgaactc gaggatcacc gggaggatgg agaagatgag gtaggacagc
     4021 agcgtcgaga tgccgcgcgt gccgcgctcg atgtcgcgcg tcatgccccc ggtctggcgc
     4081 tccaggtgga agcgcagcga cagggcgtgc aggtggcgga acacgcccag cgccacgcgg
     4141 cggatggcgc gctgcgtgac gcgcacgaac accacgtcgc gcagctcggc gaacagcgtg
     4201 gtggagaagc gcagcaggcc gtaggccgcc agcagcgccg ccggcaccgc gagcaccgcc
     4261 agcgtggggt ccaggccgtc caccacgtcc ttcatcacca gcggcacggc cacgttggcc
     4321 agcttggcgc aggtgaggag ggcgagggcc gccagcacgc gccagcggta ctcgagcagg
     4381 tagggcagca gcgtgcccag cacccgccat tcgttgccgc ggaggggctg cgccctggac
     4441 gggccgtgct cgtgggtcgc ggggttgacc atggcgggag gcgcggattc tatgccagcg
     4501 gaaccccttg acgcgccggg gtctcctttc ctactattca aacgttcgtt tgaaagggac
     4561 cgccgccatg aacctgcgcg ccatcaagcc cctgaaggaa acccagaccc ggatcctcga
     4621 cgccgccgag gagctgttca tgcagcacgg cttcgagggc gcctcgatgc gcatgctgac
     4681 cgccagggcc ggggtcaacc tcgccgcggt caactaccac ttcggctcga aggacgcgct
     4741 gatcgaggcg gtgttccgcc ggcgcctcga cccgatgaac gccgagcgcg tcgcggagct
     4801 cgagcgccgc gagcaggcgg ccgacggccg gccgctggag gccgacgcca tcatccgcgc
     4861 cttcctcggc gcgagcctgc gcatgatcga ggacacgcgc ggcggcgggc gcaccttcac
     4921 ccggctcctc gggcgcgcct acaccgagcc cgccaagccg atccggcagc tgatcggcca
     4981 gatgtacgcg ccggcgatgg agcgcttcaa ggcggccttc gtgcgcgcgc tgcccgagct
     5041 gcccaaggac gagctggtgt ggcgcatgca cttcatgttc ggcacgctcg cctacacgct
     5101 cgccgccacc gacaccgtgc agctcatcgc cggggccaag cccgaggacc gctacgacgc
     5161 gcgcgtgctc gaggatcgcc tcgcggcgtt cctggtcgcg gggctgctcg ccccgctcgc
     5221 cggttcgctg cgcgtcaaga aagccgcctg agggagcccg ccatgaccgt cttcgccgtc
     5281 gccaccttcg ccgtcctcct cctgctcggc tacttccggg cgccgatctt cgtctggagc
     5341 gccgcgctcg gcgtgatggg cgccacctgg gccgccgcgc tcggcctgcc cgcggccacc
     5401 aacgccgtgc tgttcgccgt gttcgtcgcg gcggcggcgg cctgcaacct gccggggctg
     5461 cgccggcgcc tgctcagcga cccgttgctg gcgatctacc ggcgcatcct ccccgacatg
     5521 tcgcagaccg agaaggaggc gatcgacgcc ggcaccgtgt ggtgggacgc ggatctgttc
     5581 tccggccgcc cggactggga gcggctgctg cacaccccgg cgccgcggct caacgccgag
     5641 gagcaggcct tcctcgacgg cccggtggag caggcctgcg cgatggccga cgactgggag
     5701 atcacgcacg agcgctacga cctgccgccc cagctctggc agtacctcaa ggaccagggc
     5761 ttcttcggca tgatcatccc gaagaagtac ggcggcctgg gcttctccgc gctcggccac
     5821 tcggcggtgg tgatgaagct ctcctcgcgc tccaatgcgc tcggcgtcac ggtgatggtg
     5881 cccaattcgc tcgggccggc cgagctgctg ctgcactacg gcaccgagga gcagaagaac
     5941 cactacctgc cgcgcctcgc caggggccag gaaatcccct gcttcgcgct caccagcccg
     6001 gaggcggggt cggacgcggc gtcgattccc gacttcggcg tggtgtgcaa gggcacctgg
     6061 cagggcaggg agacgctcgg catgcgcctc acctgggaca agcgctacat cacgctcggg
     6121 ccggtggcca cgctgctcgg cctcgccttc cgcctgtacg acccggaccg cctgctcggc
     6181 ggcaaggacg acctcggcat cacctgcgcc ctgatcccca ccgccacgcc cggggtgaac
     6241 atcggccggc gccacctgcc gctcaacgcg gtgttccaga acggccccaa ttcgggcaag
     6301 gacgtgttcg tgccgctgga gtggatcatc ggcgggcgcg actacgcggg caagggctgg
     6361 atgatgctga tgaactgcct cgccgccggc cgcgccatct cgctgccgac ctcctcggtg
     6421 ggcggcgcca aggcgctggc gcgcgtcacc ggcgcctacg cgcgcgtgcg cagccagttc
     6481 cgcacgccga tcggcaagct cgaaggcgtc gaggaggccc tcgggcgcat cgccgccaac
     6541 gcctacctga tggaggccac gcgcgtgatg accgcgggcg cggtggacct gggcgagaag
     6601 ccctcggtgc tctcggcgat cgccaagtac cacatgaccg agcgggcgcg cgactgcgtc
     6661 aacgacgcga tggacatcca cggcggcaag ggcatctgcc tggggcccaa caactgggtc
     6721 gggcgcggct accaggtgct gccggtgggc atcacggtgg agggcgccaa catcctcacg
     6781 cgcacgctga tcatcttcgg ccagggcgcg atccgctgcc atccctacgt gctgcgcgag
     6841 atgcgcgccg ccaaggagct gaagggcgag gaggcgtcgc gcgagttcga cgacgcgctc
     6901 acctcgcaca tcggccacac gctctccaac ggcgtgcgcg cggtggtgca cggcctcacg
     6961 cgcgcgatgt tcgccgcggc gccgctctcg gccgcgccgg agacgcgcca ctactaccgc
     7021 cacgtgacgc gcctcgcggc ggccttcgcc ttcctcgccg acgtgtcgat gctggcgatg
     7081 ggcggcgcgc tgaagcggaa ggagaagatc tccgggcggc tgggcgacgt gctctcgatg
     7141 atgtacctgg tgtcggcgac cctcaagcac tacgaggacc aggggcgcct gaaggccgac
     7201 ctgccgatgg tgcgctgggc ggtgcgcgac gcgctgtact cggcgcagca ggcgatcgac
     7261 caggtgctgt cgaatttccc cgtcaaggcg ctggcgacgc tcctgcgctg gaccatcttc
     7321 ccgctcggca tgccgttccg cccgccgctc gattcgcgca accacgagtg cgcgaagatc
     7381 gccctggaac ccggtgccgc acgcgaccgg ctgaccgccg gaatttacct ggcaaaaggc
     7441 gagaccgacg ccaccggcgt gctggagacg gcgttcctcg ccacggtggc ctgcgagccc
     7501 atcgagaaga agttccgcga tgcggtgaaa gccggcgcgc tggcggtgcg ctcgggcgtg
     7561 gacacggcgc tgatcgcgcg cgacaagggc gtgatcagcg ccgaggagta cgcgcagtgg
     7621 cagaagaagg aggcgctgcg caagcgcgtc atcatggtgg acgacttccc gcaggacttc
     7681 ggccgcgccg aaatcgtgca gaagctgcag gactaccagc cggtcaaggc aaaggcggca
     7741 tgacatgaac cccgtctacg tgatcgacgg cgccaggacg ccgttcctca agtcgaagaa
     7801 ccgccccggg cccttcgccg cgggcgacct cgcggtgcag gcggggcgga cgctcctttc
     7861 gcggcagaag ttcgcgccgc aggagctcga cgaggtgatc ctgggctgcg ccgcgccctc
     7921 ggtggacgag gtgaacatcg gccgcgtggc ggcgctgcgc atgggctgcg gcgagaaggt
     7981 gccgggctgg accgtgatgc gcaactgcgc gagcggcatg caggcgctcg actcgggcgt
     8041 gagcaacatc ctcgccgggc gctcgcagct ggtgctcgcc gggggcgtgg acgcgctgtc
     8101 gcgcgcgccg ctgctctaca acgaccggat ggtgaactgg ttctccgacc tggcggcggc
     8161 gaagaccggc ggccagcgcg cgctgctgtt cgcgcgcctg ccgttcaagg cgctgctcgc
     8221 gccggtgatc ggcatcatga aggggctcac cgacccgatg gtgggcctcc tcatgggcca
     8281 gaccgcggag aacctcgcgc accgcttcgg catcacgcgc gagcagatgg acgcctactc
     8341 ggtgcgcagc caccggcgcg tggcgcgcgc ccaggacgaa ggcccgctca aggcgggcgg
     8401 cggcgaggtc gaggcgctct acgacgccaa gggcaacgcc tacgcgctgg acgacggcgt
     8461 gcggcgcgac gccacgcccg agagcctcgc caagctgaag cccttcttcg accgcaagta
     8521 cggcaacgtc acgccgggca acagctcgca gatcaccgac ggcgcggcct ggctgatcct
     8581 cgcctccgag gaagcggtgc ggcgccacgg cctcgcgccg ctcgggcgca tcgtggatac
     8641 gcagtgggcg ggcctcgacc cggcgcagat gggcctgggg ccggtgcatg cggccacgcc
     8701 gatcctcaag cgccacggcc tggcgctgga tgacctggac tactgggaga tcaacgaggc
     8761 cttcgccgcc caggtgctgg gctgcctcgc ggcgtggaag gacgaaggct actgcaagga
     8821 gcagctgggc ctcgagcgcg cgatgggcga actcgacgcg gagaagctca acgtcgacgg
     8881 cggcgcggtg gccctcgggc acccggtcgg cgcgtcgggc gcgaggatcg tgctgcacat
     8941 gctgcagctc ctcaagcgca agaacgcgaa gcgcggcatc gcctcgatct gcatcggcgg
     9001 aggcctcggc ggcgccatgc tgctggaggc ggcctgagca tggaccgcgc gccgaacgcc
     9061 acgctcgcca agcactggcg ctgggagacc gaccgccagg gcctcgcctg gctcaccttc
     9121 gacaagcagg gcgaatccac caacacgttc tcgcgcgagg ccctggtgga actcggcgcc
     9181 gcgctcgagg ccatcgccgc ggcgcggccc agggggctcg tcatccgctc ggcgaaggag
     9241 aacttcatcg ccggggcgga catcaaccag ttcacgcgct tccagacccc ggaagaggcg
     9301 ctcgaattca cgcgcctggg ctgggacacc atgcagaagc tgcgcgacct gcccttcccc
     9361 accaccgcga tggtcaacgg cttttgcatg ggcggcgggc tcgagatggc gctcgcctgc
     9421 acctaccgcg tggcgctcga cgacccgaag acgcgcttcg ccttccccga ggtgatgctc
     9481 ggcatctggc cgttctggca cggcctgcaa tggctgccca agctcatcgg gccggcggcg
     9541 gcgctcgaca tgatcctcac cggccgcgcg gtggacgcgc ggcgcgcgaa gaaaaccggc
     9601 ctcgccaccg aggcggtgcc gctgcgcgtg ctggagaaca ccgcgcgcca ggtgacgctg
     9661 gaagcgaagc cgccgccgcg gctcgggctc ctcgagcgcc tgatgctcgg catcggccgg
     9721 cgcttcgtcg ccgcgcaggc gaggaagcag gtggcccggc gcgcgcgccg cgagcactac
     9781 ccggcgccct acgccatcct ggatgcctgg gtgaagtacg acggcgaccc gttcatcaac
     9841 cccgacgacc cggtcacctc gatcaaggcg ctctccggcc accccaccac gcggaatctc
     9901 atccgcatct tcttcctgca ggagcgcatg aagggcctcg ccaagggcgt ggacttcaag
     9961 gcgcggcagg tgcacgtgat cggcgccggc gtgatgggcg gcgacatcgc cgcggtgtgc
    10021 gccatgcgcg gcatcacggt gacgctgcag gacacctcgc ccgagcgcct cgccccggcg
    10081 gtgaagcgcg ccgccgagct gttcgcgaaa cgcctgaagg acccgcgccg cgtgcgcgac
    10141 gcgctcgacc ggctgatccc ggacgtctcc ggcgccggcg cggcgcgcgc cgacgtggtg
    10201 atcgaggcga tcttcgagaa cctgcaggcc aagcgcgagc tgttcgcgaa actcgaagcc
    10261 gtggcccggc ccgacgcggt gctcgccacc aacacctcga gcctgaagct cgccgacgtc
    10321 gcggcgcagt tcgcggaccc gtcgcggctg gtgggcatcc atttcttcaa cccggtgccg
    10381 cagctgcagc tggtggaagt ggtgtcgggc aacgccaccc gcccggaaat gaccgggcgc
    10441 gcgatcgcct tcgtgcgcca gatcgacaag ctgccgctgc cggtgcagga ctcgcccggg
    10501 ttcctcgtca accgcgtgct cggcccctac atgcagaacg ccttccggct cctcgacgag
    10561 ggcgccaggc ccgagaccat cgacgcggcg atggaggcct tcggcatgcc gatggggccg
    10621 atcgagctcg ccgacacggt aggcctggac atctgcctcg ccgccgggaa ggaactggcg
    10681 aagaaggggg cggaggcgcc gcaggtgctg ctcaacaagg tggcgctcgg gcagctcggc
    10741 aggaagaccg gccagggcat ctaccgctac gccggcggca agccggtgaa ggggcagccc
    10801 ggcgcctggc ggccggagct ggtcgaggcg ctggtcgagc cctacctcgc cgaggcgaaa
    10861 gcggtgctgg ccgaaggcat cgtcgccgac gccgagctga tcgacgcggg actcatcttc
    10921 ggcaccggct tcgcgccgtt ccgcggcggg ccgctgcact acctcgctgc gcgcaaatag
    10981 cgtgggccgc gcctcccacc agcccccggg cctcgcgctg cccaagggga agatccccgc
    11041 gctgcgcgtg gtgccgatgc cggccgacgc caaccacaac ggcgacatct tcggcggctg
    11101 gatcatggcg caggtggacg tggccggcgg cacggtcgcc gggcgggtgg cgcgcggccg
    11161 cgtggcgacg gtatcggtga actccttcgt cttcaagcag ccggtgcaga tcggcgacat
    11221 cctttcgctc tacgccgacg tggtgcgcat cggcaacacc tcgatcaccg tcaacgtcga
    11281 agtgttcgcc gagcggggcc gcgccgagtc gaaggtggtg aaagtcaccg aggcgacgct
    11341 cacctacgtc gccatcgatg cgcacggcag gccgcgcccc gtcccgaagt gagcggcacg
    11401 gcggtcgagt tcgcggcgcg cgacggcacc cggctggccg gcaccctgta ccgcccgcgc
    11461 gcgcccaacc ggcgcgcggt gctcctgcag gccgcctccg gcgtgaagca ggagtactac
    11521 ggcaagttcg ccgcctacct ggcgacgcgc ggcttcgccg ccctcacctt cgactaccgc
    11581 ggcatcgggc gctcgcgcgc ccccggcacg ttgcgcggcg tcggcgcgcg catgcgcgac
    11641 tgggcggaga aggacatcgc cggggcgctc gaccacctgg agcgcgccac ccacggcgcg
    11701 cgccttgtcg gcgtcggcca cagcttcggc ggccaggcct tcggcctggt gcccggcaac
    11761 gagcgctacg tggcggcgct caccttcggc tcgcagagcg gctactggcg gcactggagc
    11821 ggcagggagc gcgccggcat gtggttcctc agccacctgc tgctgcccgg ggtgtcccgg
    11881 gcgctgggct actttcccgc gcagcgcttc gggcagggcg aagacctgcc caagggcgtg
    11941 gcgagcgaat gggcgagctg gtgccggcac ccgggctatc tcgtcggcag gctcggcgcg
    12001 caggaggcct acgcgcaatt cagggcgccg atccgcgctt acgtggcgac ggacgacagc
    12061 tatgcgccgc cgcaggcggt cgaggctttc ctcgcgttct atccgggctc atccaggaaa
    12121 ctgatacccg tcgacccgcg ggcggcgggc ggcgagccga tcgggcactt cggctacttc
    12181 cgcgagcgcc tgcgcgacag cctgtggcgc gaggccgcgg actggcttgc aaatgcctga
    12241 tgagcgggta tctttagggc cgcgcagttc ttccgg
//
LOCUS       DASRIE010000119         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1691450, whole genome shotgun sequence.
ACCESSION   DASRIE010000119 DASRIE010000000
VERSION     DASRIE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691450"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1154)
                     /locus_tag="VFB53_05285"
     CDS             complement(123..1154)
                     /locus_tag="VFB53_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN86184.1"
                     /translation="MRPTVSDNATSQRGVAVRFGLKEKSPREEELMRVTQSIPLSAVE
                     LTAAVRDCQPYDPSRLDRVLRIDEPQGLIEVQAAARWREIAARLRPDHPHAARIGRAM
                     QTVGESLACNAAGPDGRPAVSHVESLTLVMPNGELTRVGRARQSDLFGLAIGGQGLFG
                     ALYSVTLHIGSLTRALNEATEAEVLAAPQATAARRALRVLVPPEELAAFLDEAEAHCR
                     EWRMPLAGARLRRTRKENETFLRWAQRDYAAVALGFGAPSVLGGAVRATQLRRTLLDA
                     AIARGGSFPLHCTPEATREQAEACYPQLPRFLAEKRRVDPGERITNAWYRHFRRLFGA
                     QRCVVRWSH"
     gene            1153..1896
                     /locus_tag="VFB53_05290"
     CDS             1153..1896
                     /locus_tag="VFB53_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86185.1"
                     /translation="MPAASRFVDALKKSVRARGLTYAELARRLQISEASVKRMFSRGS
                     FTLARIEEVLAAVELDLYEVARMSRGGGAGGAQLTYEQELALAKDERLLAFFWLVLNG
                     WGFGEIVDSFAIARTELTVAFARMEKLKLIEWGPRERARLLVPRDFVWRAGGPAKKAY
                     ARRAMAEFLEGRFDAPLEMLRFECRELSAESAASLKRKLERLVTEFAQCAEADSALPP
                     GRRTPVALLAGCRSWEFSAVNALKRRRAG"
     gene            complement(1893..2171)
                     /locus_tag="VFB53_05295"
     CDS             complement(1893..2171)
                     /locus_tag="VFB53_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZN86186.1"
                     /translation="MVSIRLEIAGRVQGVGFRYSMRQQAANLGVRGWVRNRSDGSVEA
                     LLQGETAAVEALVSWARRGPPGAQVTSLHREAQPGAPAHARFELRPSA"
     gene            2241..2636
                     /locus_tag="VFB53_05300"
     CDS             2241..2636
                     /locus_tag="VFB53_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86187.1"
                     /translation="MRVALLVIAGLALIGYVAVGWILPAMAGSEVRAAAQALVAGADP
                     AKQQVAAAAEKGGNLAGAGNGIKVVEKDDPKHGKMKWIVGENGAIRAWNEKNALEITL
                     TPSLQGGKTSWSCKGYPLEAMPPACGGKA"
     gene            complement(2645..>3364)
                     /locus_tag="VFB53_05305"
     CDS             complement(2645..>3364)
                     /locus_tag="VFB53_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN86188.1"
                     /translation="TQTGVMLGSPKYMSPEQVIGKRADHRSDIFSLGVILYEMLTGAA
                     PFSGENVTALMYQIVNFAPPAPSSVNTHVPEMLDFVVAKMLAKPLEERYQDARELAKD
                     LRECERSMAAPGTASLAGRPSGLASGAQPEMVRTDASNVLLAQTVNRTRQQDANPDRS
                     GTDAVPPLARGVSNSFDSNEATQRLAVLTGAATLPQTATQAVGAVPHAPRGGWRRRDW
                     LLVGGAALAGLIVAGAILKRK"
BASE COUNT          493 a         1139 c         1230 g          502 t
ORIGIN      
        1 aacggcgccg cggcctgcga agtggtgctc ggcgagggct ggaaggtgcg gcccgacagc
       61 aagctgatct ccgacctcgg ggactggctc acgcccgaga acgtggagct tgtcttcgca
      121 ggctagtgtg accagcgcac cacgcagcgc tgggcgccga acaaccttcg gaagtggcgg
      181 taccaggcgt tggtgatgcg ctcgccggga tcgacccgcc gtttctcggc gaggaagcgc
      241 ggcagctgcg gatagcaggc ctcggcctgc tcgcgggtgg cttccggcgt gcagtgcagc
      301 gggaagctgc cgccgcgggc gatggcggca tcgagcagcg tgcggcgcag ctgcgtcgcg
      361 cgcaccgcgc cgccgagcac cgaaggcgcg ccgaagccga gcgcgaccgc ggcatagtcg
      421 cgctgcgccc agcgcaggaa tgtttcgttc tcttttcgcg tgcggcgcag gcgcgcgccc
      481 gcgagcggca tgcgccattc gcggcaatga gcttcggctt cgtcgagaaa ggcggccagc
      541 tcctctggtg gcaccagcac gcgcagcgcg cggcgcgcgg ccgtagcctg cggcgcagcg
      601 aggacttcgg cctcggtggc ttcgttgagc gcgcgcgtga gcgagccgat gtgcaaggtc
      661 acgctataga gcgcgccgaa caggccttgg ccgccgatcg caagcccgaa caggtcgctc
      721 tgccgggcgc ggccgacgcg ggtcagctcg ccgttgggca tgacgagcgt caacgattcg
      781 acgtgagaca cggccggccg gccatccggc ccggcggcgt tgcaggcgag gctctcgccc
      841 accgtctgca tcgctcgccc gatacgcgcg gcgtgcgggt ggtcgggacg aaggcgcgcg
      901 gcgatctccc tccacctcgc cgcggcctgc acttcgatca ggccttgcgg ttcatcgatc
      961 cgcagcacgc ggtctaggcg tgaagggtcg tagggctggc agtcacgaac ggcggcagtc
     1021 agctccacgg cggacagcgg gatagattgc gttacgcgca taagctcctc ctctcgcggc
     1081 gatttttcct tcagtccgaa tcgcaccgca acacctcttt gtgaagtcgc gttatctgat
     1141 accgttggtc gcatgccggc ggccagccgc ttcgtcgacg cgctcaagaa gagcgtccgt
     1201 gcccgcggac tcacctacgc cgagctcgcg cgccggctgc agatttcaga agcgagcgta
     1261 aagcggatgt tctcgcgcgg cagcttcacg ctggcgcgca tcgaggaagt gctggccgcg
     1321 gtcgagctcg atctctacga ggtggcgcgg atgagccgcg gcggcggcgc cggcggcgcg
     1381 cagctcacct acgagcagga gctcgcgctg gcgaaggacg agcggctgct cgcgttcttc
     1441 tggctggtgc tgaacggctg gggcttcggc gagatcgtgg acagcttcgc catcgcgcgc
     1501 accgaactca cggtggcgtt cgcgcgcatg gagaagctga agctgatcga gtggggcccg
     1561 cgcgagcgcg cgcgcctgct cgtgccgcgc gacttcgtgt ggcgcgcggg cggtccggcg
     1621 aagaaagcct acgcgcggcg cgcgatggcg gagttcctgg agggacgatt cgacgcgccg
     1681 ctcgagatgc tgcgcttcga gtgccgcgag ctctcggccg agtcggcggc gagcctgaag
     1741 cgcaagctcg agcggctggt gacggagttc gcgcaatgcg ccgaggcgga ctcggcgctg
     1801 cctcccggcc gccgtacgcc cgtggcgctg ctcgccggct gccgctcgtg ggagttctcg
     1861 gcggtgaacg cgctcaagcg gcgccgggca ggctaggcgc tcgggcgcag ctcgaaccgc
     1921 gcgtgcgcag gcgcgccggg ctgcgcctcg cggtgcaggc tcgtcacctg cgcacccggt
     1981 ggcccgcggc gcgcccacga cacgagcgct tcgaccgccg cggtttcgcc ttgaaggagc
     2041 gcctccacgc tgccgtcgct tcggttgcgc acccagccgc gaacgcccag gttcgccgcc
     2101 tgctggcgca tcgaatagcg gaagcccacg ccttggacac ggcccgcgat ctcgagtcgg
     2161 atgctgacca ttgctgaaaa tccccggaac gctccatggt aagctgcttt gcacaagcag
     2221 caaaaagggg gggaacggga atgcgtgtcg cattgctggt gatcgccggt ctcgcgctga
     2281 tcggctacgt cgcagtgggt tggatcctgc ccgcgatggc cggatccgag gtccgggccg
     2341 ccgcacaggc cctggtcgcc ggggccgacc cggcgaagca gcaggtcgcc gcagccgcgg
     2401 agaagggcgg caacctcgcg ggagcgggca acggcatcaa ggtcgtagag aaggacgacc
     2461 cgaagcacgg caagatgaag tggatcgtcg gcgagaacgg cgcgatccgc gcctggaacg
     2521 agaagaacgc cctcgagatc acgctcacgc cgtcgctgca gggcggcaag acgagctgga
     2581 gctgcaaagg ctacccgctc gaggccatgc cgcccgcctg cggcggcaaa gcgtagcgcc
     2641 cgctttactt ccgcttgagg atcgcgccgg ccacgatcag gccggcgagc gcggcgccgc
     2701 cgaccagcag ccagtcgcgc cggcgccagc cgccgcgcgg cgcgtgcggc accgccccca
     2761 cggcctgggt cgcggtttgc gggagggtcg cggcgccggt cagcaccgcg aggcgctggg
     2821 tcgcctcgtt ggaatcgaac gaattcgata cgccgcgcgc aagcggcggc accgcgtcgg
     2881 tgccgctcct gtccgggttc gcgtcctgct gccgcgtgcg gttcacggtt tgcgccagca
     2941 gcacgttgct cgcgtcggtc ctgaccatct ccggctgcgc gccgcttgca aggccgctgg
     3001 gccggccggc cagcgaggcg gtcccgggcg ccgccatgct gcgctcgcat tcgcgcaggt
     3061 ccttcgccag ctcgcgcgcg tcctggtagc gctcttccag cggcttcgcg agcatcttcg
     3121 ccaccacgaa gtcgagcatc tcgggcacgt gcgtgttgac cgagctcggc gccggcgggg
     3181 cgaagttcac gatctggtac atgagcgcgg tgacgttctc gccgctgaag ggcgccgcgc
     3241 cggtgagcat ctcgtacagg atcacgccga gcgagaagat gtcggaacgg tggtcggcgc
     3301 gcttgccgat cacctgctcg ggcgacatgt acttcggaga gccgagcatg acgcccgtct
     3361 gggt
//
LOCUS       DASRIE010000120         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1692433, whole genome shotgun sequence.
ACCESSION   DASRIE010000120 DASRIE010000000
VERSION     DASRIE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692433"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFB53_05310"
     CDS             <1..408
                     /locus_tag="VFB53_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN86189.1"
                     /translation="SIGSRLHYWKRSAQWMARHPALGAGTGGWAEAFYEATEGDDAFM
                     HNRDRDHPHNEYVHLTVQLGPLGLVLFIALLVVAFRRAARLPGDYAALAQGFVLAFAV
                     GCLFSDFLYDTTEGHLWAVVGGALFGAAPREAA"
     gene            411..1262
                     /locus_tag="VFB53_05315"
     CDS             411..1262
                     /locus_tag="VFB53_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86190.1"
                     /translation="MASYSLLVPTWNNLPYLKLCVESLRRHSAREHQLLVHANEGSDG
                     TRAWLRGEGIEHTASDANVGICHAVNLLGARARGDYVVYLNDDMVLAPGWDTALDRAI
                     ERCRRHGAFMLSGTMVEPVDTGNVCAVHADLGRDPANFDLARFAREAPGLARADWLGA
                     TWPPTLVSRELWHQVGGYSVEFSPGMGSDNDFSRKLWQAGCRVFLGVGASLVYHFSET
                     STRRIRKNDGRRQFLMKWGMTQSGFDRHYLRRGQPAGADPVLPDPPPSFGLRWQSIKA
                     ALRRRLF"
     gene            complement(1231..2319)
                     /locus_tag="VFB53_05320"
     CDS             complement(1231..2319)
                     /locus_tag="VFB53_05320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZN86191.1"
                     /translation="MTEPGSILVISLRFLGDVLLTTPLIRSMRAAYPGAAIDALVFTG
                     MEGVLEGNPDLRSVLTTERGEPLALWRRLWRSYDLAVVAETADRPHLCGLIAGRRRVG
                     LLPPERDKAWWKRLSLSHGVVSPRDLARPVAYERLAAALGIGWKPEMVAPSAQMPEVA
                     WRDVLGFDPGRERFAVLHLAPRFRYKRWNIPGWHALMAWLHGEKLRVVITGGPGEVER
                     AYVREVLAGAKTPVTDLCGRLRFAQTADLLRRAALYVGPDTATTHLAAACGTPTVALF
                     GPTDPRLWGPHPKGGLQRPYEKVAAIQFRDKVSLLQEPSLACVPCQGEGCDKHPESHS
                     ECLDRMSAARVIDAAREALRTGAAAARP"
     gene            complement(2312..>2569)
                     /locus_tag="VFB53_05325"
     CDS             complement(2312..>2569)
                     /locus_tag="VFB53_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86192.1"
                     /translation="MTALSEQFMDNDEPNSLGAQIPAFALLDLKLAREFGWGRLALAV
                     NNALDEAYYTYAVRSNFTPDRYNVYPLPGRWLSFTAEFRVD"
BASE COUNT          363 a          937 c          889 g          380 t
ORIGIN      
        1 tccatcggct cgcgcctgca ctactggaag cgctccgcgc aatggatggc gcggcatccg
       61 gcgctgggcg ccggcaccgg cggatgggcc gaggccttct acgaggcgac cgagggcgac
      121 gacgccttca tgcacaatcg cgatcgcgat catccgcaca atgagtacgt ccacttgacc
      181 gtccagctcg ggcctctcgg cctggtcctg tttatcgccc tgctggtggt tgcgttccgg
      241 cgcgcggcgc gcctgccggg agattacgcc gcgctggcgc aggggttcgt cctcgccttc
      301 gccgtcggct gcctgttcag cgatttcctg tacgacacca ccgaaggcca cctttgggcg
      361 gtggtgggcg gggccctgtt cggcgccgcg ccgcgcgagg cggcctgagc atggcgagct
      421 attccctcct cgtccccacc tggaacaacc tgccctacct caagctctgc gtggagagcc
      481 tgcggcggca ttcggcgcgc gagcaccagc tcctggtgca cgccaatgaa ggcagcgacg
      541 gcacgcgcgc ctggctgcgc ggggaaggca tcgagcacac ggcgagcgac gccaacgtcg
      601 gcatctgcca cgccgtcaac ctcctcggcg caagagcgcg cggcgactat gtcgtctacc
      661 tcaacgacga catggtcctc gcgccgggct gggatacggc gctcgaccgc gcgatcgagc
      721 gctgccggcg ccacggcgcc ttcatgctct cgggcaccat ggtcgagccc gtggacacgg
      781 gcaacgtctg cgcggtgcac gccgacctcg gccgcgatcc ggcgaatttc gacctcgcgc
      841 gcttcgcgcg cgaggcgccc gggctcgcgc gcgccgactg gctgggcgcc acctggccgc
      901 cgacgctggt gtcgcgcgag ctgtggcacc aggtgggcgg ctacagcgtc gagttctccc
      961 ccggcatggg cagcgacaac gatttctcgc gcaagctctg gcaggcgggc tgccgcgtat
     1021 tcctcggtgt cggcgcgagc ctggtgtatc acttctcgga gacctccacg cgccgcatcc
     1081 gcaagaacga cggccggcgg cagttcctga tgaagtgggg catgacgcag tcggggttcg
     1141 accgccacta cctgcgccgc gggcagcccg ccggcgcgga tccggtgctg ccggacccgc
     1201 cgccgtcctt cggcctgcgc tggcagtcga tcaaggccgc gctgcggcgg cgcctgttct
     1261 gagggcctcg cgcgccgcgt cgatgacgcg cgccgcgctc atgcggtcca ggcactcgct
     1321 gtggctctcc gggtgcttgt cgcagccttc cccctggcag ggcacgcaag cgagcgaagg
     1381 ctcctgcagc agcgacacct tgtcgcggaa ctggatcgcc gccaccttct cgtaggggcg
     1441 ctgcaatccg cccttcggat gcggtcccca caggcgcggg tcggtcggcc cgaacagggc
     1501 caccgtcggc gtgccgcagg ccgcggcgag gtgcgtggtc gcggtgtccg gccccacgta
     1561 aagcgcggcg cggcgcagca ggtccgcggt ctgcgcgaag cgcaggcgac cgcacaggtc
     1621 tgtcaccggc gtcttcgcac cggcgagcac ttccctcacg tacgcgcgct ccacctcgcc
     1681 gggcccgccg gtgatcacca cgcgcagctt ctcgccgtgc agccaggcca tcagcgcgtg
     1741 ccagccgggg atgttccagc gcttgtagcg gaagcgtggc gcgaggtgca gcacggcgaa
     1801 gcgctcgcgc ccggggtcga accccagcac gtcgcgccag gccacctccg gcatctgcgc
     1861 gctcggcgcc accatttccg gcttccaccc gatgcccagc gccgcggcca ggcgctcata
     1921 ggccacgggc cgcgccaggt cgcgcggcga caccacgccg tgcgaaaggg aaaggcgctt
     1981 ccaccacgcc ttgtcccgct ccggcggcag caggcccacg cgccggcgcc cggcgatcag
     2041 gccgcagagg tgcggccggt cggcggtttc cgcgaccacc gcgaggtcgt agctgcgcca
     2101 caggcgccgc cacagcgcga gcggctcgcc ccgctccgtg gtgagcacgc tgcgcaggtc
     2161 ggggttgccc tccagcacgc cttccatccc cgtgaagacc agcgcatcga tcgcggcgcc
     2221 gggataggcg gcgcgcatcg agcggatgag cggcgtggtg agcagcacgt cgcccaggaa
     2281 gcgcaatgag atgacgagga tcgagccggg ctcagtcaac cctgaattcc gcggtgaagc
     2341 tcagccaccg cccgggcaac gggtacacgt tgtaccgatc aggcgtgaaa ttgctgcgca
     2401 ccgcgtaagt gtagtacgcc tcgtcgagcg cgttgttcac cgcgagcgcg aggcgccccc
     2461 agccgaattc tcgcgcgagc ttgagatcga gcagcgcgaa ggcggggatc tgcgcgccca
     2521 gggaattggg ctcgtcgttg tccatgaact gctcgctcag cgccgtcat
//
LOCUS       DASRIE010000121         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2995319, whole genome shotgun sequence.
ACCESSION   DASRIE010000121 DASRIE010000000
VERSION     DASRIE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995319"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFB53_05330"
     CDS             <1..700
                     /locus_tag="VFB53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZN86193.1"
                     /translation="YAAGKLTAIEINGTYYSTQKRESFARWREETPDDFVFSVKASRY
                     ATNRKALASAGESVERFVGSGIAELGPKLGPIVWQFMPTKRFEPDDFDAFLALLPASV
                     EGVALRHVVDVRHPSFRTPQFLAMARRHRAATVFTDSHDYPSFADLSADFVYARLMRA
                     EASQASGYAAAALDGWAQACVTWAAGGEPAELPRIGERAASDTPRDVFVFFINGAKEK
                     APAAAMALIERLRA"
     gene            complement(804..1175)
                     /locus_tag="VFB53_05335"
     CDS             complement(804..1175)
                     /locus_tag="VFB53_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86194.1"
                     /translation="MQAVDIALDDPAPRRAPANALAAWWRTLPGALRAPVWPGTLAGL
                     VILALLLGFHHVVRDAVHQGELLRMSTASRAEAVRRCNAMPRASVRASCLAQVDAPPP
                     VRGEAGPPPNTASLSLASTRS"
     gene            complement(1248..4568)
                     /gene="icmF"
                     /locus_tag="VFB53_05340"
     CDS             complement(1248..4568)
                     /gene="icmF"
                     /locus_tag="VFB53_05340"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HZN86195.1"
                     /translation="MTDLSAEYKALAEYRPNHKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVIHLGHNRSVDEIVTAALQEDVQGIAVSSYQGGHVEYFKYMVDLLKSRGGAHI
                     QVFGGGGGVIVPAEIAELQGYGVARIYSPEDGQRMGLAGMIGEMVMRCDGDLSAYAPK
                     KLDAIRGDTEAHWRALAQLVTALEEGKVSDALKKSLHEEAKAHRTPVVGITGTGGAGK
                     SSLTDELIRRLRLDQDDRLRIAVVSIDPSRRKSGGALLGDRIRMNAISPWRRPGADAG
                     ASAGARVFMRSMATREGGSEVSPALADVVAACKCAGFDLVVVETSGIGQGDAAIVPLV
                     DVPVYVMTPEYGAASQLEKIDMLDFAEYVVINKFDRKGAPDALRDVAKQVQRNREAFK
                     ERPDEMPVFGTMASRFNDDGVTALYHALLPRLVELGLKAGESRLPRVETRHSTHQVPI
                     VPPARVRYLAEIADGVRGYKRRAREQARLAREAQQLRESARMLGAAKCDAPGAQDALQ
                     QLAAQREGGLDAHAAKQLAMWPDMQKAYAGDEYVVRIRDKELRTALTHTTLSGTKIRK
                     VALPQYEDHGEILKWLLLDNVPGSFPYTAGTFAFKREGEDPTRMFAGEGDAFRTNRRF
                     KLLSEGMPAKRLSTAFDSVTLYGNDPDPRPDIYGKVGNSGVSIATLEDMKVLYSGFDL
                     CSPSTSVSMTINGPAPTILAMFMNTAIDQQVQKFRDDNGREPTDGEAQKIRAWVLQNV
                     RGTVQADILKEDQGQNTCIFSTEFSLKMMGDIAEWFVHHQVRNFYSVSISGYHIAEAG
                     ANPISQLAFTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVLGRVARRIW
                     AVAMRDRYGANERSQKLKYHVQTSGRSLHAQEIQFNDIRTTLQALIAIYDNCNSLHTN
                     AFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGAFIIDELTELVEEAVLAEF
                     EKIAERGGVLGAMETGYQRGKIQEESLHYEMLKHTGEYPIIGVNTFRNPHGDKVPEHI
                     ELARSTEEEKQSQLKRLADFHARHANDAPVLLKRLQQAVIDNRNVFEVLMDAVRACSL
                     GQITNALFEVGGQYRRSM"
     gene            complement(4600..4764)
                     /locus_tag="VFB53_05345"
                     /pseudo
     CDS             complement(4600..4764)
                     /locus_tag="VFB53_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--nucleotide phosphotransferase"
     gene            4810..>5107
                     /locus_tag="VFB53_05350"
     CDS             4810..>5107
                     /locus_tag="VFB53_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZN86196.1"
                     /translation="MATPPAAAHTVSAAFVASAVARLVPTVRKRVLAACGIAPQVLGA
                     PGARVPAAAFGALWMAVAHELDDEFFGLDARRMKVGSFALLCRALLSHATVGGAM"
BASE COUNT          660 a         1788 c         1815 g          844 t
ORIGIN      
        1 gtacgccgcc ggcaagctga ccgccatcga gatcaacggc acctactaca gcacgcagaa
       61 gcgcgagagc ttcgcccggt ggcgcgagga gacgcccgac gatttcgtct tctcggtcaa
      121 ggcctcgcgc tacgccacca accgcaaggc gctcgccagc gcgggcgagt cggtcgagcg
      181 cttcgtcggc agcggcatcg ccgagctcgg ccccaagctc gggccgatcg tctggcagtt
      241 catgccgacc aagcgcttcg agcccgacga tttcgacgcc ttcctcgcac tgctgccggc
      301 gtcggtcgag ggcgtggcgc tgcgccacgt ggtggacgtg cggcatccga gctttcgcac
      361 gccgcaattc ctggcgatgg cacggcggca tcgggccgcc acggtgttca ccgattcgca
      421 cgactacccc tcgttcgccg atctctcggc cgatttcgtc tacgcgcggc tcatgcgcgc
      481 cgaggcgtcg caagccagcg gctacgccgc ggcggcgctg gacggctggg cccaggcctg
      541 cgtcacctgg gcagcgggcg gcgagccggc cgagctgcct cgcatcggcg agcgcgcggc
      601 gagcgacacg ccgcgcgacg tgttcgtctt cttcatcaac ggtgcgaagg agaaggcgcc
      661 ggcggcggcg atggcattga tcgagcgcct gcgcgcgtag ggcgcgcaac agggccgggc
      721 cgcacgggtt tgccgcgcat cggcggctcg cccgcgctgg cggcgcgccc gcgtgccccc
      781 cggcagagcc ccggcagccg cggctacgag cgcgtgcttg cgaggctcag cgaggcggtg
      841 ttgggcgggg ggccggcctc gccgcgcacc ggcggcggag cgtcgacctg cgccaggcag
      901 ctcgcccgca ccgacgcgcg cggcatggcg ttgcaccgcc gcacggcctc ggcgcggctg
      961 gccgtgctca tgcgcagcag ttcgccctgg tgcaccgcgt cgcgcacgac gtggtggaag
     1021 ccgagcagca gcgcgaggat caccaggccc gccagcgtcc ccggccacac cggggcgcgc
     1081 aaggcgccgg gcagggtccg ccaccaggcg gcgagagcgt ttgcgggggc ccggcgcggg
     1141 gccgggtcgt ccagggcgat gtcgacggct tgcatggtgc cgccatggtg ccgccgcgcc
     1201 cgcatgcggc gtatcggggg agcgaccgaa acgctgtagg agcgggacta catgctgcgc
     1261 cggtactgcc cgcccacctc gaacagcgcg ttcgtgatct gccccagcga gcacgcccgc
     1321 accgcgtcca tcagcacctc gaacacgttg cggttgtcga tcaccgcctg ctgcagccgc
     1381 ttcagcagga ccggcgcgtc gttcgcgtgg cgtgcgtgga aatcggccag gcgcttcagc
     1441 tgcgactgct tctcctcctc ggtggagcgc gccagctcga tgtgctcggg caccttgtcg
     1501 ccgtgcggat tgcggaaggt gttcacgccg atgatcgggt actcgccggt gtgcttgagc
     1561 atctcgtagt gcaggctctc ctcctggatc ttgccgcgct ggtagcccgt ctccatcgcg
     1621 cccagcacgc cgccgcgctc ggcgatcttc tcgaactcgg cgagcaccgc ctcttccacc
     1681 agctcggtga gctcgtcgat gatgaacgcg ccctggttcg ggttctcgtt cttcgccagg
     1741 ccccactcgc ggttgatgat gagctggatc gccatcgcgc ggcgcacgct gtcctcggtg
     1801 ggcgtggtga tcgcctcgtc gaacgcattg gtgtgcagcg agttgcagtt gtcgtagatc
     1861 gcgatcagcg cctgcagcgt ggtgcggatg tcgttgaact ggatctcctg cgcgtgcagc
     1921 gagcggcccg acgtctgcac gtggtacttg agcttctggc tgcgctcgtt ggcgccatag
     1981 cggtcgcgca tcgccaccgc ccagatgcgc cgcgccacgc ggccgagcac ggtgtactcg
     2041 gggtccatgc cgttgctgaa gaagaacgac aggttgggcg cgaagtcgtc gatgtgcatg
     2101 ccgcgcgcga ggtacgcctc gacgaacgtg aagccgttcg agagcgtgaa ggcgagctgc
     2161 gagatcgggt tggcgccggc ctcggcgatg tggtagccgg agatcgacac cgaatagaag
     2221 ttgcgcacct ggtggtgcac gaaccactcg gcgatgtcgc ccatcatctt caggctgaac
     2281 tcggtggaga agatgcaggt gttctggccc tggtcctcct tgaggatgtc ggcctgcacg
     2341 gtgccgcgca cgttctgcag cacccaggcg cggatcttct gcgcctcgcc gtcggtgggc
     2401 tcgcggccgt tgtcgtcgcg gaacttctgc acctgctggt cgatcgcggt gttcatgaac
     2461 atcgcgagga tcgtgggcgc ggggccgttg atcgtcatcg acaccgaggt gctcgggctg
     2521 cacaggtcga agccggagta cagcaccttc atgtcctcga gcgtggcgat cgacacgccg
     2581 gagttgccca ccttgccgta gatgtccggc cgcgggtcgg gatcgttgcc gtagagcgtg
     2641 acgctgtcga aggcggtgga cagccgcttg gccggcatgc cctcggacag cagcttgaag
     2701 cgccggttgg tgcggaacgc gtcgccttcg ccggcgaaca tgcgcgtcgg gtcctcgccc
     2761 tcgcgcttga acgcgaaggt gccggcggtg tacgggaagc tgcccggcac gttgtcgagc
     2821 agcagccact tgaggatctc gccgtggtcc tcgtactgcg gcagcgcgac cttgcggatc
     2881 ttggtgcccg acagcgtggt gtgggtgagc gcggtgcgca gctccttgtc gcggatcctc
     2941 accacgtact cgtcgcccgc gtaggccttc tgcatgtcgg gccacatcgc gagctgcttg
     3001 gccgcgtgcg catcgagccc gccctcgcgc tgcgcggcga gctgctgcag cgcgtcctgc
     3061 gcgccggggg cgtcgcactt cgccgcgccc agcatgcgcg cgctctcgcg cagctgctgc
     3121 gcctcgcgcg cgaggcgcgc ctgctcgcgc gcgcggcgct tgtagccgcg cacgccgtcg
     3181 gcgatctcgg cgaggtagcg cacgcgcgcc ggcggcacga tcggcacctg gtgggtgctg
     3241 tggcgggtct cgacgcgcgg caggcggctc tccccggcct tcaggcccag ttccaccagc
     3301 cgcggcagca gcgcatggta gagcgccgtc acgccgtcgt cgttgaagcg gctcgccatg
     3361 gtgccgaaca ccggcatctc gtcgggccgc tccttgaacg cctcgcggtt gcgctgcacc
     3421 tgcttggcca cgtcgcgcag cgcgtccggc gcgcccttgc ggtcgaactt gttgatgacc
     3481 acgtactcgg cgaagtcgag catgtcgatc ttctcgagct ggctcgccgc gccgtattcc
     3541 ggcgtcatca cgtagaccgg cacgtcgacc agcggcacga tcgccgcgtc gccctggccg
     3601 atgcccgagg tctcgacgac caccaggtcg aagcccgcgc acttgcaggc ggcgaccacg
     3661 tcggcgagcg ccgggctcac ctcgctgccg ccttcgcgcg tggccatgct gcgcatgaag
     3721 acgcgcgcgc ccgccgacgc gcccgcgtcg gctcccggcc tgcgccacgg ggagatggcg
     3781 ttcatgcgga tgcggtcgcc cagcagtgcg ccgccgctct tgcgccgcga cgggtcgatg
     3841 ctgacgacgg cgatgcgcag ccggtcgtcc tggtccagcc gcagccgccg gatcagctcg
     3901 tcggtgagcg acgacttgcc cgcgccgccg gtgccggtga tgccgaccac gggcgtgcga
     3961 tgcgccttcg cctcctcgtg cagcgacttc ttcagcgcgt cggagacctt gccttcctcc
     4021 agcgcggtga cgagctgggc gagggcgcgc cagtgcgcct cggtgtcgcc gcggatcgca
     4081 tcgagcttct tcggcgcgta cgccgagagg tcgccgtcgc agcgcatcac catctcgccg
     4141 atcatgccgg ccaggcccat gcgctggccg tcctccgggc tgtagatgcg cgccacgccg
     4201 tagccctgga gctcggcgat ctcggccggc acgatcacgc cgccgccccc gccgaacacc
     4261 tggatgtgcg cgccgccgcg cgacttcagc aggtccacca tgtacttgaa gtactcgacg
     4321 tggccgccct ggtacgagct caccgcgatg ccctgcacgt cctcctgcag cgcggcggtg
     4381 acgatctcgt cgaccgagcg gttgtgcccg aggtggatca cctcggcgcc catgccctgc
     4441 aggatgcgcc gcatgatgtt gatggccgcg tcgtggccgt cgaacagcga cgcggcggtg
     4501 acgaagcgca ccttgtggtt ggggcggtac tcggcgaggg ccttgtattc ggcggacagg
     4561 tcggtcatgt cgtctccgtg cgcgcggcgc ggcgcgccgt cattcgtcga tggtcttcgg
     4621 caggtcggcc ggggccgggg ggtagcgcag cccgagtccc tcgagcgtct gcttcacgac
     4681 cgtcgcgacc atcaggttgc ggtgggtctt ggaatcggcc ggcacgatgg tccacggcgc
     4741 ccacggagtg ccggtggccg cgatggccga accgatcacc cgcccgaccg catttgccag
     4801 aatcggtgcg tggccacccc tccagccgcc gcacacacgg tgtccgccgc cttcgtggcg
     4861 agcgcggttg cgcggctcgt ccccacagtg cgcaagcgcg tgttggccgc ctgtggcatc
     4921 gcgccgcagg tgctgggcgc gccgggcgcg cgcgtgcctg cggcggcgtt cggcgcgttg
     4981 tggatggcgg tggcgcacga actggacgac gaattcttcg gcctcgatgc gcgccgcatg
     5041 aaagtgggca gcttcgcgct gctgtgccgc gcactgctga gccacgccac cgtgggcggt
     5101 gcaatgc
//
LOCUS       DASRIE010000122         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1440088, whole genome shotgun sequence.
ACCESSION   DASRIE010000122 DASRIE010000000
VERSION     DASRIE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440088"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFB53_05355"
     CDS             complement(<1..301)
                     /locus_tag="VFB53_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZN86197.1"
                     /translation="MTIVGTRPEIIKLSCVIPELDRAFRHVLVHTGQNYAYELNQIFF
                     EGLGLRKPDHFLEAAGENAAQTIANVIARADAAMAKEKPDAVLILGDTNSGLAALA"
     gene            complement(306..1316)
                     /locus_tag="VFB53_05360"
     CDS             complement(306..1316)
                     /locus_tag="VFB53_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZN86198.1"
                     /translation="MFKDKTLLITGGTGSFGNAVLAYFLDTEFAEIRVFSRDEKKQED
                     MRLHFRNDKLKFYIGDVRDYDSVHDALRGVDYVFHAAALKQVPSCEFYPMEALRTNVI
                     GAENVMRAAIANGVRRCVVLSTDKAVYPINAMGLSKAMMEKVMVAKARLCEPSSTVLC
                     ATRYGNVMASRGSVIPLFLGQLRDGKPLTVTDPAMTRFLMSLEESVALVLYAFEHARP
                     GDIFVQKAPASTVGDLAQALRELTRRDNPIQVIGTRHGEKLYESLVSREEMARAEDRG
                     DYYRIPADTRDLNYDKFFVEGETAISRIDDYTSHNTRRLDVEAVKQVLMQLAMVREAV
                     RA"
     gene            complement(1350..2219)
                     /locus_tag="VFB53_05365"
     CDS             complement(1350..2219)
                     /locus_tag="VFB53_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86199.1"
                     /translation="MAESRPTVLVLGASGMLGHAALRLFAESPGHAVWGTVRGHAAPA
                     ALPAALHERIVTGVDVENPDSLARAFAVTRPDVVINAVGLVKQLAVADDPLQAIPINA
                     LLPHRLARLCEVAGARLVHVSTDCVFTGTRGNYREDDPADAQDLYGRSKHLGEVDAPH
                     AITLRTSIIGHELGAAHGLVGWFLAQSGTVKGFTRAVFSGLPTVELARVIRDHVLPRP
                     ELRGLWHVSAAPISKHDLLRLVAEVYGRATTIVPDERLVINRSLDSARFRRATGYEPP
                     AWPELVARMHAFG"
     gene            complement(2212..3471)
                     /locus_tag="VFB53_05370"
     CDS             complement(2212..3471)
                     /locus_tag="VFB53_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86200.1"
                     /translation="MNVLILSQHFWPETFRINEIAHSLRAAGCRVSVLTGQPNYPDGD
                     VFPGYRAWRAGVEDYDGVPVYRVPLAPRGRSGALRLAGNYLSFLLSASVAGPWLLRGR
                     RFDVVFVYGTSPILQVLPALVLRAFKGCAVVTWVQDLWPQSLQVTGYVRHPRVLAMVA
                     GVVRWLYRRSDLLLVQSRGFLSSVQPMAGGTPVEVHPNPGEVAFERAPDGAPALALEP
                     GFNVVFAGNLGTVQALDTVVAAAALLRDEPHVRFVLVGSGSRGEALAAQAQRLGLANV
                     AFPGRFPVDAMPGILAQASALLVSLKRDDILAQTVPSKVQAYLAAGRPVIASLDGEGA
                     RVVEEAGAGIACPAEDAQALADAVRRLAGAPPAELAAMGGRARAYYQTHFEPAMLAER
                     LARRFAQLAGVPPSAAPLPERIGSDHG"
     gene            complement(3468..4259)
                     /locus_tag="VFB53_05375"
     CDS             complement(3468..4259)
                     /locus_tag="VFB53_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HZN86201.1"
                     /translation="MLRPRIIACLLVHEGGLVKTTKFRDPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATATGAEPNVRLISQFAAECRMPLCYGGGVKTAEQAKRIIGLGVEKVAI
                     SSAAIERPELIAEIAEAVGSQSVVVVLDAKKKLLGGWEVWTHNGRKATGRSVADMAKL
                     AAERGAGEVVVNSIDQDGVMSGYDLALARKVREAIRLPISVLGGAGSLADLGTLIAEC
                     GVVGAAAGSLFVFKGAYRAVLISYPAPAQKEELVRSNLACAGPRR"
     gene            complement(4261..4872)
                     /gene="hisH"
                     /locus_tag="VFB53_05380"
     CDS             complement(4261..4872)
                     /gene="hisH"
                     /locus_tag="VFB53_05380"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZN86202.1"
                     /translation="MITIVDYGLGNILAFQNVYKRLNVECTIARRADDLRGATRLILP
                     GVGAFDHAMELLDASGMRAPVDELVLDKRVPVLGICVGMQILARASEEGSAPGLGWIA
                     GRVRGFRSLGRDDLLLPHMGWNDVRPARPSPLFAGLETDARFYFLHSFFFECERAEDV
                     AATSAYGTDFASAVHAGNIHGVQFHPEKSHHFGTQLLKNFAEL"
     gene            complement(4869..>5371)
                     /locus_tag="VFB53_05385"
     CDS             complement(4869..>5371)
                     /locus_tag="VFB53_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS biosynthesis protein"
                     /protein_id="HZN86203.1"
                     /translation="KTFPQSDIFTYKLYYRYVKGVKVLRPLNYVPYIKEAAMQLLIDR
                     FGWQRYAHKHYESRFTRFYEGYWLPTKFGYDKRRAHFSSLILTGQMTREEALEKIAKP
                     AYDEATIAQDFEYIATKLDITVDELRALHRGPNKTFRDYKSAMPLIHLGTQVMRAFGV
                     QRAIIR"
BASE COUNT          786 a         1867 c         1923 g          795 t
ORIGIN      
        1 cggcgagcgc ggcgaggccg ctgttggtgt cgccgaggat cagcaccgcg tcgggcttct
       61 ccttcgccat cgccgcgtcg gcgcgggcga tgacgttggc gatggtctgc gcggcgttct
      121 cgcccgccgc ctcgaggaaa tggtcgggct tcctcagccc gaggccctcg aaaaagatct
      181 ggttcagctc gtaggcgtag ttctggccgg tgtgcaccag gacatgccgg aacgcgcggt
      241 cgagctcggg gatgacgcag gagagcttga tgatctccgg ccgcgtgccg acgatcgtca
      301 tcaccttaag cacgcacggc ctcccgcacc atcgcgagct gcatcagcac ctgcttgacc
      361 gcctccacgt ccagccggcg cgtgttgtgc gaggtgtagt cgtcgatgcg cgagatcgcc
      421 gtctcgccct cgacgaagaa cttgtcgtag ttgaggtcgc gcgtgtcggc cgggatgcgg
      481 tagtagtcgc cccggtcctc ggcgcgcgcc atctcctcgc gcgacaccag cgactcgtac
      541 agcttctcgc cgtggcgcgt gccgatcacc tggatcgggt tgtcgcgccg cgtcagctcg
      601 cgcagcgcct gcgccaggtc gcccacggtg gaggcgggcg ccttctgcac gaagatgtcg
      661 ccggggcgcg cgtgctcgaa cgcgtacagc accagcgcca ccgattcctc gagcgacatg
      721 aggaagcgcg tcatcgccgg gtcggtcacc gtgagcggct tgccgtcgcg cagctgcccg
      781 aggaacagcg ggatcaccga gccgcgcgag gccatcacgt tgccgtagcg cgtggcgcac
      841 agcaccgtgg acgacggctc gcacaggcgc gccttggcga ccatcacctt ctccatcatc
      901 gccttggaca ggcccatggc gttgatcggg tacaccgcct tgtcggtgct cagcaccacg
      961 cagcggcgca cgccgttggc gatcgccgcg cgcatcacgt tctccgcgcc gatcacgttg
     1021 gtgcgcagcg cctccatcgg atagaactcg cacgagggca cctgcttcag cgccgcggca
     1081 tggaacacgt agtccacgcc gcgcagggcg tcgtgcacgc tgtcgtagtc gcgcacgtcg
     1141 ccgatgtaga acttcagctt gtcgttgcgg aaatgcaggc gcatgtcctc ctgcttcttc
     1201 tcgtcgcggc tgaacacgcg gatctccgcg aactcggtgt ccaggaagta ggccaggacg
     1261 gcgttgccga acgagccggt gccgcccgtg atgagcagcg tcttgtcttt gaacatggag
     1321 aaggtcctgg cgagtcggtg aggagagggt cagccgaagg cgtgcatgcg cgcgaccagc
     1381 tcgggccagg ccggcggctc gtagccggtg gcccggcgaa agcgcgcgga gtcgagcgag
     1441 cggttgatga cgaggcgctc gtccgggacg atcgtcgtgg cgcggccgta cacctcggcc
     1501 acgaggcgca gcaggtcgtg cttgctgatg ggcgcggccg acacgtgcca gagcccgcgc
     1561 agctcgggtc gcggcagcac gtggtcgcgg atcacgcgcg ccagctccac cgtcggcagc
     1621 ccggagaaca cggcgcgcgt gaagcccttc accgtgccgc tttgcgcgag gaaccagccg
     1681 accaggccgt gcgccgcgcc gagctcgtgg ccgatgatcg acgtgcgcag cgtgatcgcg
     1741 tgcggcgcgt ccacctcccc caggtgcttg ctgcggccgt agaggtcctg cgcgtcggcc
     1801 ggatcgtcct cgcggtagtt cccgcgcgtg ccggtgaaca cgcagtcggt gctgacgtgc
     1861 acgaggcgcg cgcccgccac ttcgcacagc cgcgccagcc ggtgcggcag cagcgcgttg
     1921 atggggatgg cctgcagcgg atcgtcggcc actgcgagct gcttgaccag gccgacggca
     1981 ttgatcacca cgtcgggccg cgtcacggcg aacgcgcgcg ccaggctgtc ggggttctcc
     2041 acgtccacgc ccgtgacgat gcgctcgtgc agcgcggcgg gcagcgccgc cggcgccgcg
     2101 tggccgcgca ccgtgcccca caccgcgtgg cccgggctct cggcgaacag gcgcagcgcg
     2161 gcatggccca gcatgccgga ggcgccgagg acgaggacgg tggggcggga ctcagccatg
     2221 gtcggatccg atccgttcgg gaagcggcgc ggcggacggg ggcacgcccg cgagctgcgc
     2281 gaagcggcgc gccagccgct cggccagcat cgcgggttcg aagtgggtct ggtagtacgc
     2341 gcgggcgcgc ccgcccatcg ccgcgagctc ggccggcggt gcgccggcca gccggcgcac
     2401 cgcgtcggcc agcgcctgcg catcctcggc cgggcaggcg atgccggcgc ccgcctcctc
     2461 gaccacgcgc gcgccttcgc cgtcgagcga ggcgatcacc gggcgcccgg cggcgaggta
     2521 ggcctgcacc ttgctcggca ccgtctgcgc gaggatgtcg tcgcgcttga ggctcaccag
     2581 cagcgccgag gcctgcgcca ggatgccggg catcgcgtcg accgggaagc ggccggggaa
     2641 ggcgacgttg gcgaggccga ggcgctgcgc ttgcgcggcg agcgcctcgc cgcggctgcc
     2701 gctgcccacg agcacgaagc gcacgtgcgg ctcgtcgcgc agcagcgccg cggccgcgac
     2761 caccgtgtcg agcgcctgca cggtgccgag gttgccggcg aacaccacgt tgaagccggg
     2821 ctcgagcgcg agcgccggcg cgccgtcggg cgcgcgctcg aacgccacct cgcccgggtt
     2881 cggatgcacc tcgaccggcg tgccgccggc catcggctgc acggacgaga ggaagccgcg
     2941 cgactgcacc agcagcaggt cgctcctgcg gtagagccag cgcacgacgc cggccaccat
     3001 cgcgagcacg cgcggatggc gcacgtagcc ggtgacctgc aggctttgcg gccacaggtc
     3061 ctgcacccac gtgacgacgg cgcagccctt gaaggcgcgc agcaccagcg cgggcagcac
     3121 ctgcaggatc ggcgaggtgc cgtagacgaa gacgacgtcg aagcgccggc cgcgcagcag
     3181 ccaggggccc gcgacgctcg cgctgagcag gaacgacagg tagttgcccg ccagccgcaa
     3241 ggcgccggag cggccgcgcg gcgcgagcgg cacgcggtac accggcacgc cgtcgtagtc
     3301 ctcgacgccc gcgcgccacg cgcggtagcc ggggaacacg tcgccgtccg ggtagttcgg
     3361 ctgccccgtc agcacgctca cccggcagcc ggccgcgcgc agcgaatgcg cgatctcgtt
     3421 gatgcggaag gtctcgggcc agaagtgctg gctgaggatg agcacgttca tcgtcgcggg
     3481 ccggcgcacg ccaggttcga ccggacgagc tcttccttct gcgcgggcgc cgggtagctg
     3541 atgagcaccg cgcggtaggc gcccttgaag acgaacaggc tgccggccgc ggcgccgacc
     3601 acgccgcact cggcgatcag cgtgccgagg tcggccagcg agccggcgcc gccgagcacc
     3661 gagatgggca ggcggatcgc ctcgcgcacc ttgcgcgcga gcgcgaggtc gtagccggac
     3721 atcacgccgt cctggtcgat cgagttgacc accacctcgc cggcgccgcg ctcggccgcg
     3781 agcttggcca tgtcggcgac cgaccggccg gtcgccttgc ggccgttgtg cgtccacacc
     3841 tcccagccgc ccagcagctt cttcttcgcg tcgagcacga cgaccacgct ctggctgccg
     3901 accgcctcgg cgatctcggc gatcagctcc ggccgctcga tggcggccga gctgatcgcc
     3961 accttctcga cgcccaggcc gatgatgcgc ttggcctgct cggcggtctt cacgccgccg
     4021 ccgtagcaca gcggcatgcg gcactcggcc gcgaactgcg agatgaggcg cacgttgggc
     4081 tcggcgccgg tggcggtggc gtcgatgtcg agcaccatca gctcgtcagc ttccttctcg
     4141 ttgaagatct tgacggcgtt gatcgggtcg ccgacgtact tcggatccct gaacttcgtc
     4201 gtcttgacca gaccgccttc gtgcaccagc aggcaggcga tgattcgggg gcgcagcatc
     4261 tcagagctcc gcgaagttct tcaggagctg ggtcccgaag tggtggctct tttccgggtg
     4321 gaactgcacg ccgtggatgt tgccggcgtg caccgcgctc gcgaagtcgg tgccgtacgc
     4381 cgaggtggcg gccacgtcct cggcgcgctc gcactcgaag aagaacgaat gcaggaaata
     4441 gaagcgcgcg tcggtctcga ggcccgcgaa cagcggcgag ggacgggccg ggcgcacgtc
     4501 gttccatccc atgtgcggca gcagcaggtc gtcgcggccg agcgagcgaa agccgcgcac
     4561 gcgcccggcg atccagccga gccccggcgc gcttccctcc tcactcgcgc gcgcgaggat
     4621 ctgcatgccg acgcagatgc ccagcaccgg cacgcgcttg tcgagcacca gctcgtcgac
     4681 cggcgcgcgc atgcccgagg cgtccagcag ctccatcgcg tggtcgaacg cgcccacgcc
     4741 gggcaggatg agccgggtgg cgccgcgcag gtcgtccgcg cgccgcgcga tcgtgcactc
     4801 gacgttcagc cgcttgtaga cgttctggaa cgccaggatg ttgccgaggc cgtagtcgac
     4861 gatggtgatc atcggatgat cgcccgctgc acgccgaagg cgcgcatgac ctgggtgccg
     4921 aggtggatca gcggcatcgc cgacttgtag tcgcggaagg tcttgttcgg cccgcggtgc
     4981 agcgcgcgca gctcgtcgac agtgatgtcg agcttggtgg cgatgtactc gaagtcctgc
     5041 gcgatggtgg cctcgtcgta cgccggcttc gcgatcttct cgagcgcctc ctcgcgcgtc
     5101 atctggccgg tgaggatcag gctggagaag tgggcgcgcc gcttgtcgta gccgaacttg
     5161 gtgggcagcc agtagccctc gtagaagcgc gtgaagcgcg actcgtagtg cttgtgcgcg
     5221 tagcgctgcc agccgaagcg gtcgatcagc agttgcatcg ccgcctcctt gatgtaggga
     5281 acgtaattca gcggccgcag caccttcacg cccttgacgt agcggtagta gagcttgtac
     5341 gtaaagatgt ccgactgcgg aaaggtcttg a
//
LOCUS       DASRIE010000123         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3249279, whole genome shotgun sequence.
ACCESSION   DASRIE010000123 DASRIE010000000
VERSION     DASRIE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249279"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFB53_05390"
     CDS             complement(<1..1459)
                     /locus_tag="VFB53_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HZN86204.1"
                     /translation="MNQVVDAGVLAEASQITDAKQRYSAGVLKYRQMGYWDPAYEPKD
                     TDVLCLFRITPQEGVDPVEAAAAVAGESSTATWTVVWTDRLTACDTYRAKAYQVEPVP
                     GTPGQYFAWVAYDLVLFEEGSIANMTASLIGNVFSFKPLKAARLEDIRIPVAYVKTFK
                     GPPTGLVVERERLDKFGRPLLGATTKPKLGLSGRNYGRVIYEGLKGGLDFMKDDENIN
                     SQPFMHWRDRFLYVMDAVNKASAATGEVKGSYLNVTAGTMEEMYERAEFAKELGSVVV
                     MVDLVIGWTAIQSMAHWARRNDIILHMHRAGHGTYTRQKNHGVSFRVIAKWLRLAGCD
                     HLHAGTAVGKLEGDPLTVQGYYHVCRDAFTQQDYARGLYFDQDWADLRKVMPVASGGI
                     HAGQMHQLVDLFGDDVVLQFGGGTIGHPAGIQAGAVANRVALEAMVKARNEGRDIATE
                     GPRILQDAARFCTPLKQALDTWKDVSFNYTPTDTSD"
     gene            1581..2510
                     /locus_tag="VFB53_05395"
     CDS             1581..2510
                     /locus_tag="VFB53_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN86205.1"
                     /translation="MNVSFRQLRVFTEVVRQGSVQRAAAALHLTPPAVSMQLKEMESQ
                     VGLALFDREGRRLTLSTAGEYFAFYARRLLATLKEAEDAMARFKGVASGVLTIGMVGP
                     AQYFLPQLLGQFHAEHPAVDVRLRLGNREQLVAAMTASEVDLSVMGRAPKEWPNRAEP
                     FAAHPHVLITAPDHPFARSESVPAAALARERFIVREPGSGSRAAVAEYLAEHRIEPVV
                     AMELPSDEAIKRTVMAGLGVALVSLHTIGLEWRNGLLAAPHVEGLPLMRRWHLVNAFA
                     KALSPAAEAFRYFMLERAEAALADMFGGELAIG"
     gene            2673..3263
                     /locus_tag="VFB53_05400"
     CDS             2673..3263
                     /locus_tag="VFB53_05400"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HZN86206.1"
                     /translation="MNHPLRCRCGNLRGQVVLPATTARAVCYCKDCRAFAHFLQRPDD
                     ILDAQGGTDIVATPPSHVRFEHGADALACMSLSPRGLLRWYAGCCKTPVGNTPRDRRT
                     HYVGLVAACLAAQPLEPSFGPARVRLNTGSARGPVESTRAGTLLAVMKIMAFMLPARF
                     SARQRDNPFFDAASGEPVKRPDVLSRDEAARLKALP"
     gene            complement(3325..>4142)
                     /locus_tag="VFB53_05405"
     CDS             complement(3325..>4142)
                     /locus_tag="VFB53_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86207.1"
                     /translation="ARLRDEVRGRGPQDLTPIATGAVPREVAPLIDAINAHTERQREL
                     AEAQARFVANASHQLKTPLTVLRAQAAHALMQTEVDAMRRIVARMDATTEDTGRMVEQ
                     LLALARSEPGRALQLRDVDLTELAREATFDLLATARASRIDLGFEGDAPVPVRGEPLL
                     LREMVANLVHNAIVYTGARGSVTVAVGHRARRPVLTVVDNGPGIAPAERPRVLERFYR
                     APGCGAAGSGLGLAIVREICARHGIELLLEDAPGGGSGLCVRLVWPEAAAAAA"
BASE COUNT          543 a         1490 c         1474 g          635 t
ORIGIN      
        1 aatcgctggt gtcggtgggc gtgtagttga aggacacgtc cttccacgtg tcgagcgcct
       61 gcttcagcgg cgtgcagaag cgcgcggcgt cctgcaggat gcgcgggccc tcggtggcga
      121 tgtcgcggcc ctcgttgcgc gccttgacca tcgcctccag cgcgacgcgg ttggccaccg
      181 cgcccgcctg gatgccggcc gggtggccga tcgtgccgcc gccgaactgc agcaccacgt
      241 cgtcgccgaa gaggtccacg agctggtgca tctgccccgc atggatgccg ccggaggcca
      301 cgggcatcac cttgcggaga tccgcccagt cctggtcgaa gtagaggccg cgcgcgtagt
      361 cctgctgcgt gaacgcgtcg cggcacacgt ggtagtagcc ctgcacggtg agcgggtcgc
      421 cctcgagctt gcccaccgcg gtgcccgcgt gcaggtggtc gcagcccgcc agccgcagcc
      481 acttggcgat cacgcggaag ctcacgccgt ggttcttctg ccgcgtgtac gtgccgtggc
      541 cggcgcggtg catgtgcagg atgatgtcgt tgcgccgcgc ccagtgggcc atcgactgga
      601 tcgcggtcca gccgatcacc aggtccacca tcacgaccac cgagcccagc tccttcgcga
      661 actcggcgcg ctcgtacatc tcctccatcg tgccggcggt cacgttgagg tacgagccct
      721 tcacctcgcc ggtggccgcg ctcgccttgt tcaccgcatc catcacgtag aggaagcggt
      781 cgcgccagtg catgaagggc tgcgagttga tgttctcgtc gtccttcatg aagtcgagcc
      841 cgcccttgag gccttcgtag atcacgcggc cgtagttgcg gcccgacagg cccagcttcg
      901 gcttggtggt ggcgcccagc agcgggcggc cgaacttgtc caggcgctcg cgctcgacca
      961 ccaggccggt gggcgggccc ttgaaggtct tcacgtacgc gaccgggatg cggatgtcct
     1021 cgaggcgcgc cgccttcagc ggcttgaacg agaacacgtt gccgatgagg ctcgccgtca
     1081 tgttggcgat ggagccttcc tcgaacagga cgaggtcgta cgccacccac gcgaagtact
     1141 gccccggcgt gccgggcacc ggctccacct ggtaggcctt ggcgcggtag gtgtcgcagg
     1201 cggtgaggcg gtcggtccac accaccgtcc aggtggcggt gctcgattcg ccggccaccg
     1261 cggcggccgc ctccaccggg tccacgccct cctggggcgt gatgcggaac aggcacagga
     1321 cgtcggtgtc cttcggctcg tacgccgggt cccagtagcc catctgccgg tacttgagga
     1381 cgccggcgct gtagcgctgc ttcgcgtcgg tgatctggct ggcctcggcg agcacgccgg
     1441 cgtcgacgac ctggttcatg gggatccctc gcaggtggag aaagaatcga tgccatgcac
     1501 tctaggaacc catgctctta aggtaaagtc aaactttctt cgtcgttgat taagtatttc
     1561 cttaacatcg ggtaaacgcg atgaacgtgt ctttccggca gcttcgggtt ttcactgagg
     1621 tggtccggca gggcagcgtg cagcgcgcgg ccgcggcgct gcacctgacg ccgcccgccg
     1681 tctccatgca gctgaaggag atggagtcgc aggtcggcct cgcgctgttc gaccgcgaag
     1741 gccggcggct cacgctctcc accgccggcg agtacttcgc cttctacgcg cggcgcctgc
     1801 tcgccacgct gaaggaggcg gaagacgcga tggcgcgctt caagggcgtc gcctcgggcg
     1861 tgctcaccat cggcatggtg ggtccggcgc agtacttcct gccccagctg ctcgggcagt
     1921 tccatgccga gcatcccgcg gtcgacgtgc ggctgcggct cggcaaccgc gagcagctcg
     1981 tcgcggcgat gacggccagc gaggtcgacc tctccgtgat gggccgcgcg cccaaggaat
     2041 ggcccaatcg cgccgagccg ttcgccgcgc atccgcacgt cctgatcacc gcgccggacc
     2101 acccgttcgc gcgctcggag agcgtgcccg ccgccgcgct cgcgcgcgag cgcttcatcg
     2161 tgcgcgagcc gggctccggc tcgcgcgccg cggtggcgga gtacctcgcg gagcaccgca
     2221 tcgagccggt cgtcgcgatg gagctgccga gcgacgaggc gatcaagcgc acggtgatgg
     2281 cggggctcgg cgtggcgctg gtgtcgctgc acaccatcgg gctcgagtgg cgcaacgggc
     2341 tgctcgcggc gccgcatgtc gagggtctgc cgctgatgcg ccgctggcac ctcgtcaacg
     2401 cgttcgcgaa ggcgctgtcg ccggcggcgg aggcattccg ctacttcatg ctggaacggg
     2461 cggaggcggc gctggcggac atgttcggcg gggagctggc gatcggatag gtgcctgcgg
     2521 cggcagcggc gcaagcgcag cgggatcgcg cgttcgcgga tccgcagaag ccgccggcgg
     2581 tgccgccgcc ggtcccgggg accgcacccc gcagacgcgc cgcgccgcac gccgcgttgg
     2641 accgcagccc acgccgtgtg taggctcgag ccatgaacca tccgctgcga tgccggtgcg
     2701 gcaacctgcg cggccaggtc gtgctgcccg ccacgacggc gcgcgccgtc tgctattgca
     2761 aggactgccg ggccttcgcc cacttcctcc agcgcccgga cgacatcctc gatgcgcaag
     2821 gcgggacgga catcgtcgcc acgccgccgt cgcacgtgcg cttcgagcac ggcgccgacg
     2881 ccctggcctg catgtccttg tcgccgcggg gcctgctgcg ctggtacgcc ggctgttgca
     2941 agacgccggt cggcaacacg ccgcgcgatc gccgcacgca ctacgtgggc ctggtcgccg
     3001 cctgcctggc cgcccagccg ctcgagccgt cgttcggccc ggcgcgcgtc aggctgaaca
     3061 ccggctcggc gcgcggcccg gtcgagtcca cgcgcgcagg caccctgctg gcggtgatga
     3121 agatcatggc gttcatgctt cccgcgcgat tcagcgcgcg ccagcgcgac aacccgttct
     3181 tcgacgccgc cagcggcgaa cccgtcaagc gccccgacgt gctgagccgc gacgaggcgg
     3241 cgcggctgaa ggcgctgccg tgaacatcgc gcgggcccgg cccgcgccca tcggctgcag
     3301 gtgagtcgct cgcgcgggcc gagctcatgc cgccgcggcc gccgcttccg gccagaccag
     3361 ccgcacgcac agcccgctgc cgccgccggg cgcgtcctcg agcagcagct cgatgccgtg
     3421 gcgcgcgcag atctccctca cgatggcgag cccgagcccg ctgcccgccg cgccgcaacc
     3481 cggggcgcgg tagaagcgtt ccagcacgcg cggccgctcg gcgggcgcga tgccgggacc
     3541 gttgtcgacc acggtcagca ccggccgccg cgcgcgatgc ccgaccgcga cggtcacgct
     3601 gccgcgcgcg cccgtgtaca cgatggcgtt gtgcacgagg ttggcgacca tctcgcgcag
     3661 cagcaggggc tcgccgcgga ccggcaccgg cgcgtcgccc tcgaagccca ggtcgatgcg
     3721 gctggcgcgc gccgtcgcga gcaggtcgaa ggtcgcctcg cgcgccagtt ccgtgaggtc
     3781 cacgtcccgc agctgcagcg cgcggcccgg ctcgctgcgc gccagggcga ggagctgctc
     3841 caccatgcgg ccggtgtctt cggtggtcgc gtccatgcgc gcgacgatgc ggcgcatcgc
     3901 gtcgacctcg gtctgcatca gcgcgtgcgc ggcctgcgcg cgcaggacgg tgagcggcgt
     3961 cttcagctgg tgggaggcat tggcgacgaa gcgcgcctgc gcctcggcga gctcgcgctg
     4021 gcgctcggtg tgggcgttga tggcgtcgat gagcggcgcc acctcgcgcg gcaccgcgcc
     4081 ggtcgcgatg ggcgtgaggt cctgcggccc gcgcccgcgc acctcgtcgc gcagcctggc
     4141 ga
//
LOCUS       DASRIE010000124         8034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2752888, whole genome shotgun sequence.
ACCESSION   DASRIE010000124 DASRIE010000000
VERSION     DASRIE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752888"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFB53_05410"
     CDS             <1..549
                     /locus_tag="VFB53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN86208.1"
                     /translation="GFGFDASGPEIPRFTEALALNAGMVVADVGAGKGQLTRALAGTV
                     GPGGHVFSTEIDPDRLQALRAMLAEARLGNVTIVEAKARDSGLPAACCDAIVLRRVYH
                     HVTDPAETNASLLRALRPGGLLAVIDFPPPFFSGRGNLGVPAQSVIDEVTASGFKLLQ
                     LRRDWPGRGPLETYCALFRKPL"
     gene            753..986
                     /locus_tag="VFB53_05415"
     CDS             753..986
                     /locus_tag="VFB53_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZN86209.1"
                     /translation="MPLSEVKDDLSRFLREAETQDVVITRHGKPAGVLIGFKSEDDWF
                     DYRLENDPRFLQRVEQARASFRAGRGVKLEDVK"
     gene            complement(998..1074)
                     /locus_tag="VFB53_05420"
     tRNA            complement(998..1074)
                     /locus_tag="VFB53_05420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1038..1040),aa:Arg,seq:ccg)
     gene            1124..1627
                     /locus_tag="VFB53_05425"
     CDS             1124..1627
                     /locus_tag="VFB53_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZN86210.1"
                     /translation="MSKHDEHSSFIKTPQQLLVVVLLAFLVPIIAILLLVSLVLNQPG
                     ADAAALRPEAVAERLQPVGRVEFGAPAAAPGERTGEEIVKGTCGTCHVPGVANAPKIG
                     DKAAWGALAKQGLPTLLRDAIKGVGAMPPRGGLADLSDTELARAIVYMANQSGAGLKE
                     PAAPKKK"
     gene            1662..2606
                     /gene="gshB"
                     /locus_tag="VFB53_05430"
     CDS             1662..2606
                     /gene="gshB"
                     /locus_tag="VFB53_05430"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZN86211.1"
                     /translation="MNLLFVIDPIEELKAYKDTTVCMMRAAQRRGHQVHVCGQADLAW
                     RSATVAAKALRIALADNDEDWYRRDGSAERALGEYGAVLMRKDPPFDMEYVASTWLLS
                     EAERQGARVFNAPRALRDHNEKFAIAEFARYSVPSLVTRDMAALQAFIDEQRDAVLKP
                     LSGMGGTGIFRVRRDDPNRNVIVETLAALGASSVMAQRYIPEIAQGDKRILVIEGRPA
                     PYVLARIPKAGESRGNLAAGARGVAQPLSARDREIAEALGPALAARGILFAGLDAIGD
                     WLTEVNVTSPTCFREIQDQTGYDAGAVFIEALEARVKP"
     gene            2603..3010
                     /locus_tag="VFB53_05435"
     CDS             2603..3010
                     /locus_tag="VFB53_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZN86212.1"
                     /translation="MIGVLVVSHGAIAEALLSSAEQILGAKQAQVAALGVSRNDDPDR
                     VLEKARGIVARLEQGQGVLVLTDMFGATPCNVAARLLADGRVEGVSGVSLPMLVRVLA
                     GRNGSLPAAVQRALSGGAEGVVHMNSDSCGAKR"
     gene            2997..3266
                     /locus_tag="VFB53_05440"
     CDS             2997..3266
                     /locus_tag="VFB53_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZN86213.1"
                     /translation="MPSAELTIANKLGLHARASAKLTQVASGFAAEVWLTRNGRRVNA
                     KSIMGVMMLAAGKGATVTVEAEGADADAALAAVARLIADKFDEGE"
     gene            3271..4995
                     /gene="ptsP"
                     /locus_tag="VFB53_05445"
     CDS             3271..4995
                     /gene="ptsP"
                     /locus_tag="VFB53_05445"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZN86214.1"
                     /translation="MNFVLHGSSVASGITIGHAHLVSSARLDVAHYAIPASQVPAEIF
                     RFDAGIARAKQELAQLESQMPQDAPAEFGAFLNLHRMILEDSSLSHAPRELIRERRCN
                     AEWALVQQMETLVEQFEAMEDSYLRERKADIEQVVERVLKALIGGQAPAEPQLSEEHK
                     LIVVAHDLSPADMLLFKRHEFGGFVTDLGGVTSHTAILARSLDIPALVGLRHARSMIR
                     EGELLIVDGIQGVLVIDPDPIVLAEYQLRQSQHDLEKKKLRRLKSTPAATLDGTAVEL
                     YANIELPQDLDEVHESGAQGVGLFRTEFLFMNRKDLPGEDEQFEAYREVARAMDGKPV
                     VIRTLDVGADKALSGHGGTMPNPALGLRAIRFCLAEPQMFLEQLRAILRASHYGRVRI
                     LLPMVAHAHEVDQALALLRQARQQLDEQGLPYDKGVEVGGMIEIPAAALSLPMFMKRL
                     DFLSLGTNDLIQYTLAIDRTDDAVAHLYDPLHPAVLHLVAHVIHAATRGGTPVAVCGE
                     MAGDLALTRLLLGFGLRNFSMHPAQLPAIKERVLRSSLAAAQPLAQKVLRSADAGKTR
                     ELLAKLNA"
     gene            5004..6197
                     /locus_tag="VFB53_05450"
     CDS             5004..6197
                     /locus_tag="VFB53_05450"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN86215.1"
                     /translation="MPGPLHGVRVLDLTTVVMGPYATQILADFGADVVKVEPPEGDIM
                     RQVWPFRSQGMGHIFLNANRNKRGIVLDLKRPEAREACLALAKRADVLVYNIRPQAMA
                     RLKLGYEEVRAANPRIIYVGAFGYSQRGPYAAKAAYDDLIQGAAGLPWLAREAGAESP
                     RYTPVIIADRSVGLHVASAVSAALYAREKTGKGQRVDVPMFESLLQSVLGEHLGGYTF
                     EPQLGEGGYRRMLTEFRRPYATRDGYVCALIYNDKHWKAFFELIGRPDMLADARFADP
                     EGRSRNYDAVYGFVADEMHKRSTEEWLAALEAADIPVQRMNSLEDILKDPHLAAIGYF
                     QEVEHPSEGRLRTMQVPSEWSETAPEYRRHAPRLGEHTREVLREAGYTDAQIEAMAAT
                     GAARL"
     regulatory      6197..6274
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            6320..7429
                     /locus_tag="VFB53_05455"
     CDS             6320..7429
                     /locus_tag="VFB53_05455"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZN86216.1"
                     /translation="MIVQAQKARFTEPLRLRSGAVLPAYEIAYETYGTLNAQRSNAVL
                     VCHALNASHHVAGSYAGEPDSLGWWDNMVGPGKPLDTDRFFVVGSNYIGSCFGSTGPA
                     SPNPATGRPWGADFPVVTVEDWVAAQAQLADRLGIERFAAVMGGSLGAMQALQWTLSY
                     PGRVRHAIVIAATPRLSAQNIAFNEVARQAIMTDPDFHGGHYYDKGVVPVRGLRIARM
                     IGHITYLSDDAMMEKFGRALRGKNGPRFDFDVDFEIESYLRYQGDKFSTYFDANTYLR
                     ITKALDYYDPAADYGGDLSAAFARAEADFLVVSFTSDWRFTSQRSREIVRALLDNRRV
                     VSYLENDAPQGHDAFLLDDARYHGALRAYFGNIPL"
     gene            7426..8001
                     /gene="metW"
                     /locus_tag="VFB53_05460"
     CDS             7426..8001
                     /gene="metW"
                     /locus_tag="VFB53_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZN86217.1"
                     /translation="MTETEVIAGWIAPKSRVLDLGCGNGTLLRHLWDKRQAPGYGVEI
                     DDAHVLECIRNDVNVLQMDLEDGLAAFADRSFDTVILSETLQAIHRQEPLLRDMLRVG
                     REAVVSFPNFGYWRARIQIALAGHMPVSRELPYQWYDTPNVHHCTLVDFEALCAKLDM
                     RILERVVLRQGTRVSVLPNLLGSTALYRVTA"
BASE COUNT         1274 a         2807 c         2800 g         1153 t
ORIGIN      
        1 ggattcggct tcgatgcgag cggccccgaa ataccgcggt tcacggaggc gctcgcatta
       61 aacgccggaa tggtggtcgc agatgtcggc gccggcaagg gtcagctgac gcgtgcgctg
      121 gcgggaacgg tcgggccggg cggacatgtc ttctcgaccg agatcgatcc ggatcgcctg
      181 caagccctgc gcgcaatgct cgccgaagca aggcttggca acgtcacgat cgtcgaagcg
      241 aaggcacgcg actccggact gccggcagcc tgctgcgacg cgatcgtgct gcgccgcgtc
      301 taccatcacg tgaccgatcc ggcggaaacc aacgcgagcc tgctgcgcgc gctgcgcccc
      361 ggcgggctgc tggcggtgat cgattttccg ccgccgtttt tctccggtcg ggggaacctc
      421 ggggttccgg cgcaaagcgt catcgacgag gtcacggcga gcggcttcaa actgctccaa
      481 ctgcgcaggg actggccggg ccgcggtcct ctagagacct actgcgcgct gttccgcaaa
      541 cccctataaa gtgctctgca cgaacccacc aagaccagcc ggagccgaat caagcggttg
      601 cgcggattag cacggccgca gtaccggctc cgggtcgagg aggtccgtgt gttctacgat
      661 gtatcgagtt cgacagtcga ggtactggcg atcgtgccca aaccggaggc tgagtcatgg
      721 ttggcgcagt tcggaaatcc aaattgaagg aagtgcctct gtccgaggtc aaagacgacc
      781 tgtcccgatt tctccgggaa gccgagacgc aggacgtcgt catcacgcgc cacggcaaac
      841 cagccggcgt gcttatcggc ttcaagtcgg aggacgactg gttcgactat cggcttgaaa
      901 acgatccccg gttcctgcag cgcgtcgaac aggcccgcgc cagcttccgc gcgggccggg
      961 gcgtcaagct cgaagacgtg aaatagtccg gcggatctgg cgcgcccggc gggattcgaa
     1021 cccacgaccc ttggcttcgg aggccaatac tctgatccgc tgagctacgg gcgctttcac
     1081 gctaaaatcg ccgcaattct aatcccttac cccggccccg cgcatgagca agcacgacga
     1141 gcattcctcc ttcatcaaga cgccgcagca gctgctggtg gtggtgctgc tcgccttcct
     1201 ggtgccgatc atcgccatcc tgctgctggt cagcctggtg ctgaaccagc cgggcgccga
     1261 cgccgcggcg ctcaggcccg aagcggtggc cgagcgcctg cagccggtgg gccgcgtgga
     1321 gttcggcgcg cccgccgccg cgccgggcga gcgcacgggc gaggagatcg tcaagggcac
     1381 ctgcggcacc tgccatgtgc cgggcgtggc caatgcgccg aagatcggcg acaaggctgc
     1441 ctggggtgcg ctcgccaagc agggcctgcc gacgctcctg cgcgatgcga tcaagggcgt
     1501 cggcgccatg ccgccgcgcg gcgggcttgc cgatctctcc gacaccgagc tggcgcgcgc
     1561 catcgtctac atggcgaatc aatccggcgc cggcctgaag gaacccgccg cgccgaagaa
     1621 gaagtaagcg gccccgcccc gggccgcggc gagctgcagc agtgaacctc ctgttcgtca
     1681 tcgacccgat cgaggagctg aaggcctaca aggacacgac cgtgtgcatg atgcgcgcgg
     1741 cccagcgccg cggccaccag gtgcacgtct gcggccaggc ggacctcgcc tggcgctcgg
     1801 cgacggtggc ggcgaaggcc ctgcgcatcg cgctcgccga caacgacgaa gactggtacc
     1861 ggcgcgacgg cagcgccgag cgcgccctcg gcgagtacgg cgcggtgctg atgcgcaagg
     1921 acccgccctt cgacatggag tacgtggcga gcacctggct cctgtcggag gccgagcggc
     1981 agggcgcgcg cgtgttcaac gcgccgcggg cgctgcgcga ccacaacgag aaattcgcca
     2041 tcgccgagtt cgcccgctac agcgtaccct cgctggtgac gcgcgacatg gccgcgctgc
     2101 aggccttcat cgacgagcag cgcgatgcgg tgctgaagcc cctctcgggc atgggcggca
     2161 ccggcatctt ccgcgtgcgc cgcgacgacc ccaaccgcaa cgtgatcgtc gagacgctcg
     2221 ccgcgctcgg cgcctcgagc gtgatggcgc agcgctacat cccggagatc gcgcagggcg
     2281 acaagcgcat cctggtcatc gagggcaggc ccgcgcccta cgtactggcg cgcatcccca
     2341 aggccggcga gtcgcgcggc aacctcgccg ccggcgcccg gggcgtggcg cagccgctgt
     2401 cggcgcgcga ccgcgagatc gccgaggcgc tcgggcccgc gctcgcggcg cgggggatcc
     2461 tgttcgccgg gctggacgcg atcggcgact ggctcaccga ggtcaacgtc accagcccga
     2521 cctgcttccg cgagatccag gaccagaccg gctacgacgc cggcgcggta ttcatcgagg
     2581 cgctggaagc cagggtgaag ccgtgatcgg cgtgctggtg gtgtcgcacg gcgccatcgc
     2641 cgaggcgctg ctctccagcg ccgagcagat cctgggggcg aagcaggcgc aggtcgccgc
     2701 gctgggcgtg tcgcgcaacg acgacccgga ccgcgtgctg gagaaggcgc gcggcatcgt
     2761 cgcgcgcctg gagcagggcc agggggtgct ggtgctcacc gacatgttcg gcgccacgcc
     2821 ctgcaacgtc gccgcgcggc tgctcgccga cggccgcgtc gagggcgtct cgggggtatc
     2881 gctgccgatg ctggtgcgcg tgctcgccgg gcgcaacggc tcgctgccgg cggcggtgca
     2941 gcgcgcgctc tcgggcggcg ccgaaggcgt ggtgcacatg aattcggaca gctgcggtgc
     3001 caagcgctga gctcaccatc gccaacaagc tcggcctgca cgcgcgcgcc tccgccaagc
     3061 tgacccaggt ggccagcggc ttcgccgccg aggtctggct gacgcgcaac ggccggcgcg
     3121 tgaacgccaa gagcatcatg ggcgtgatga tgctggccgc cggcaagggc gccacggtca
     3181 ccgtcgaggc cgagggcgcg gatgccgacg ccgcgctcgc cgccgtcgcc cgcctgatcg
     3241 ccgacaagtt cgacgaaggg gagtaggacg gtgaatttcg tcctgcacgg ctcgagcgtc
     3301 gcctcgggca tcaccatcgg ccacgcgcac ctcgtctcca gcgcccgcct ggacgtggcg
     3361 cactacgcga tccccgcgtc ccaggtgccg gccgagatct tccgcttcga cgccggcatc
     3421 gcgcgcgcca agcaggagct ggcgcagctg gagagccaga tgccgcagga cgcgcctgcc
     3481 gagttcggcg ccttcctcaa cctgcaccgc atgatcctgg aggactcgtc gctgtcgcac
     3541 gcgccgcgcg aactcatccg cgagcgccgc tgcaacgccg agtgggcgct ggtgcagcag
     3601 atggagacgc tggtcgagca gttcgaggcc atggaggaca gctacctgcg cgagcgcaag
     3661 gccgacatcg agcaggtggt ggagcgcgtg ctgaaggcgc tgatcggcgg ccaggcgccg
     3721 gccgagccgc agctgtcgga ggagcacaag ctgatcgtgg tggcgcacga cctgtcgccc
     3781 gccgacatgc tgctgttcaa gcgccacgag ttcggcggct tcgtcaccga cctcggcggc
     3841 gtcacctcgc acaccgcgat cctggcgcgc agcctggaca tcccggcgct ggtcggcctg
     3901 cgccacgcgc gcagcatgat ccgcgagggc gagctgctga tcgtcgacgg catccagggc
     3961 gtgctggtga tcgacccgga cccgatcgtg ctcgccgagt accagctgcg ccagtcgcag
     4021 cacgacctgg agaagaagaa gctgcgccgc ctgaagagca cgccggcggc gacgctcgac
     4081 ggcacggcgg tggagctgta cgccaacatc gagctgccgc aggacctgga cgaagtgcac
     4141 gagtcgggcg cgcagggcgt cggcctgttc cgcaccgagt tcctgttcat gaaccgcaag
     4201 gacctgccgg gcgaggacga gcagttcgag gcctaccgcg aggtggcgag ggccatggac
     4261 ggcaagccgg tggtgatccg cacgctggac gtcggcgccg acaaggcgct cagcgggcac
     4321 ggcgggacga tgcccaaccc ggccctcggc ctgcgcgcga tccgcttctg cctcgccgag
     4381 ccgcagatgt tcctcgagca gctgcgcgcg atcctgcgcg cttcgcacta cgggcgcgtg
     4441 cggatcctgc tgccgatggt ggcgcacgcg cacgaggtgg accaggcgct ggcgctcctc
     4501 aggcaggcgc ggcagcagct cgacgagcag ggcctgccct acgacaaggg cgtcgaggtc
     4561 ggcggcatga tcgagatccc ggcggcggcg ctgtcgctgc cgatgttcat gaagcggctg
     4621 gacttcctgt cgctcggcac caacgacctg atccagtaca ccctggccat cgaccgcacc
     4681 gacgacgcgg tggcgcacct gtacgacccc ctgcaccccg cggtgctgca cctggtcgcg
     4741 cacgtcatcc acgccgccac gcgcggcggc acgccggtgg cggtgtgcgg cgagatggcg
     4801 ggggacctgg cgctcacgcg attgctgctc ggcttcgggc tgcgcaattt ctccatgcac
     4861 ccggcgcagc tgccggcgat caaggagcgc gtgctgcgct ccagcctcgc cgcggcgcag
     4921 ccgctggcgc agaaggtgct gcgctcggcg gacgcgggga agacgcgcga actgctggcg
     4981 aaactgaacg cctgagaggc aagatgcccg gtcccctgca cggcgtgcgc gtcctcgacc
     5041 tcaccacggt ggtgatgggc ccctatgcca cgcagatcct cgccgacttc ggcgccgacg
     5101 tggtgaaggt ggagccgccc gagggggaca tcatgcgcca ggtctggccg ttccgctccc
     5161 agggcatggg gcacatcttc ctcaacgcca accgcaacaa gcgcggcatc gtgctggacc
     5221 tgaagcgccc cgaggcgcgc gaggcctgcc tggcgctggc gaagcgtgcc gacgtgctgg
     5281 tgtacaacat ccgcccgcag gcgatggcgc gcctgaagct cggctacgag gaggtgcgcg
     5341 cggcgaaccc gcgcatcatc tacgtcggcg ccttcggcta cagccagcgc ggcccctacg
     5401 cggcgaaggc cgcctacgac gacctgatcc agggcgccgc cggcctgccc tggctcgcgc
     5461 gcgaggccgg cgcggagtcg ccgcgctaca ccccggtgat catcgccgac cgctcggtcg
     5521 gcctgcacgt ggcgagcgcg gtgagcgcgg cgctgtatgc gcgcgagaaa accggcaagg
     5581 ggcagcgcgt ggacgtgccg atgttcgaga gcctgctgca gtcggtgctc ggcgagcacc
     5641 tgggcggcta caccttcgag ccgcagctcg gcgaaggcgg ctaccggcgc atgctcaccg
     5701 agttccgccg gccctatgcc acgcgcgacg gctacgtctg cgcgctgatc tacaacgaca
     5761 agcactggaa ggcgttcttc gagctgatcg gccgcccgga catgctcgcc gacgcgcgct
     5821 tcgccgaccc cgaggggcgc agccggaact acgacgcggt gtacggcttc gtcgccgacg
     5881 agatgcacaa gcgcagcacc gaggaatggc tcgccgccct ggaggcggcc gacatcccgg
     5941 tgcagcgcat gaacagcctg gaggacatcc tcaaggaccc gcacctcgcc gccatcggct
     6001 acttccagga agtcgagcac ccgagcgaag gccggctgcg cacgatgcag gtgccctcgg
     6061 agtggtcgga gaccgcgccc gagtaccggc gccacgcgcc gcgcctgggc gagcacacgc
     6121 gcgaggtgct gcgcgaggcg ggctacaccg acgcgcagat cgaggcgatg gcggcgacgg
     6181 gggcggctcg gctataatcc gcgccagcgc cgatttgatg ttcagcttgc gggcgctcta
     6241 caaatgccgc taaagagacc ggcccgaaga gcccgttctc gcgaaagctg aacgggcttt
     6301 ttcattgggc gggcgcagca tgatcgtaca ggcgcagaag gcgcgcttca ccgagcccct
     6361 ccggctgcgg agcggcgccg tgctgcccgc ctacgagatc gcctacgaga cctacggcac
     6421 gctgaacgcg cagcgctcca acgcggtgct ggtgtgccat gcgctcaacg cctcgcacca
     6481 cgtcgccggc agctacgccg gcgagccgga cagcctcggc tggtgggaca acatggtggg
     6541 cccgggcaag ccgctcgaca ccgaccgctt cttcgtcgtc ggctccaact acatcggctc
     6601 ctgcttcggc tccaccgggc cggcgtcgcc caacccggcc accggcaggc cctggggcgc
     6661 ggattttccc gtggtcacgg tggaggactg ggtggccgcg caggcgcagc tcgccgaccg
     6721 cctcggcatc gagcgcttcg ccgcggtgat gggcgggtcg ctgggcgcga tgcaggcgct
     6781 gcagtggacg ctctcctacc ctgggcgcgt gcgccatgcg atcgtcatcg ccgcgacgcc
     6841 ccggctctcg gcgcagaaca tcgcgttcaa cgaggtggcg cgccaggcga tcatgaccga
     6901 cccggacttc cacggcgggc actactacga caagggcgtg gtgccggtgc gcgggctgcg
     6961 catcgcgcgc atgatcggcc acatcaccta cctgtcggac gacgccatga tggagaagtt
     7021 cggccgggcg ctgcgcggca agaacggccc gcgcttcgac ttcgacgtgg atttcgagat
     7081 cgagagctac ctgcgctacc agggcgacaa gttctccacc tacttcgacg ccaacaccta
     7141 cctcaggatc accaaggcgc tggactacta cgacccggcg gcggactacg gcggcgacct
     7201 gtcggccgcg ttcgcgcgcg ccgaggccga tttcctggtg gtgtccttca cctcggactg
     7261 gcgcttcacc tcgcagcgct cgcgcgaaat cgtgcgggcg ctgctggaca accggcgcgt
     7321 ggtgtcctac ctggagaacg acgcgccgca ggggcacgac gccttcctgc tggacgatgc
     7381 gcgctaccac ggcgcgctgc gcgcctattt cggcaacatc ccgctgtgac cgagaccgag
     7441 gtcattgcag gctggatcgc ccccaagtcc cgggtgctcg acctgggttg cggcaatggc
     7501 actcttctca ggcacttgtg ggataagcgg caggcgccgg gctacggcgt ggagatcgac
     7561 gacgcgcacg tgctcgagtg catccgcaac gacgtcaacg tgctgcagat ggacctggag
     7621 gacggcctcg ccgcgttcgc cgaccggtct ttcgacaccg tcatcctgtc ggagacgctg
     7681 caggcgatcc accgccagga gccgctgctg cgcgacatgc tgcgcgtggg gcgcgaggcg
     7741 gtggtttcgt tcccgaactt cggctactgg cgcgcgcgca tccagatcgc cctcgccggc
     7801 cacatgccgg tgtcgaggga acttccctac cagtggtacg acacccccaa cgtgcaccac
     7861 tgcacgctgg tggacttcga ggcgctgtgc gcgaaactcg acatgcgcat cctcgagcgc
     7921 gtggtgctgc gccagggtac gcgcgtcagc gtgctgccca acctgctggg cagcaccgcg
     7981 ctgtaccgcg tcactgctta gcggcgccgc aggcctccgc ctcgcgctgg cagg
//
LOCUS       DASRIE010000125         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_910646, whole genome shotgun sequence.
ACCESSION   DASRIE010000125 DASRIE010000000
VERSION     DASRIE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910646"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFB53_05465"
     CDS             <1..1050
                     /locus_tag="VFB53_05465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86218.1"
                     /translation="VQDNANQLERLEGAASVECLHARRYLDFKAIKRLAVLLSRIRPS
                     AVIAANPYAMLYATLALRHAGLNAPLMVTYHTTLLAGAKEWLQMLYYRPFFWGADCLV
                     FVCEAQRRHWLRRLVSGRANRVIHNGVDPEYWSAPAAPARTRMRRVLGLADGDYAIGI
                     CAVFRPEKNHLQLVEAVAQLRARGLPARALLIGDGPMRPAIEARARDLGVAGDVLIAG
                     FHQDVRPLLAASDVVALPSTSVETFSLAALEAMALGRPVVLSEIGGAAEMVRPDENGF
                     LFPAHDTPALVAALGALADPAARERMGAAARSTVEARFSERAMVDRYEGLLQQLVLTR
                     NQRDNLRKSAAAHQG"
     gene            1010..2041
                     /locus_tag="VFB53_05470"
     CDS             1010..2041
                     /locus_tag="VFB53_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86219.1"
                     /translation="MTTYEKVQQRIRAERRTWLVTGVAGFIGSNLAVKLLELGQDVVG
                     LDNFSTGKRDNIPAGVRFIEGDIRSLDACREACKGVERVLHQAALGSVPRSIDRPLDT
                     HDNNVNGFLNMLVAARDAGVPRMVFASSSAVFGDDPILPKVEERIGRAASPYGLSKHV
                     NELYAGVFATCYGFKWIGLRYFNVFGPRQSPDGPYASVIPAWIGALLRGHTAYINGDG
                     SAARDFCHIDNVVQANLLSAMTEDPAAIDQAYNVAHGEMTTLNELYEIIRTLLAKRMP
                     HLASVRAVHREPRRGDLLLSRADTGKAARLLGYRPTKGVMDGLEATMDWYLANLQPAR
                     EETKEVVNG"
     gene            2034..>2359
                     /locus_tag="VFB53_05475"
     CDS             2034..>2359
                     /locus_tag="VFB53_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86220.1"
                     /translation="MAEVLEKIETRSEAPAALATVAVVGLGYVGLPVAVAFGKQRRTI
                     GYDLSKRRVENLKHMVDTTGEVSTAELIEARHLKASAHAADLAHADFIIVAVPTPINA
                     ARQPDLS"
BASE COUNT          361 a          856 c          820 g          322 t
ORIGIN      
        1 gtgcaggaca acgccaacca gctcgagcgc ctggaaggcg cggccagcgt ggaatgcctg
       61 cacgcccgga ggtatctgga tttcaaggcc atcaagcggc ttgcggtcct gctgtcgcgt
      121 atccgcccct cggcggtgat cgcggcgaat ccctacgcca tgctctacgc cacgctcgcg
      181 ctgcgccacg ccgggctcaa cgcgccgctg atggtgacgt accacaccac gctgctcgcc
      241 ggcgccaagg aatggctgca gatgctctat taccggccgt tcttctgggg cgccgactgc
      301 ctggtgttcg tgtgcgaggc gcagcgccgc cactggctgc gccgcctggt gagcggccgc
      361 gcgaaccgcg tgatccacaa cggcgtcgac cccgagtatt ggagcgcgcc ggcggcgccg
      421 gcgcgcacgc gcatgcgccg cgtgctggga ctcgccgacg gcgactacgc gatcggcatc
      481 tgcgcggtgt tccgcccgga gaagaaccac ctgcagctgg tggaggcggt ggcgcagctg
      541 cgcgcccgcg gccttccggc gcgcgcgctg ctcatcggcg acgggccgat gcgcccggcg
      601 atcgaggcgc gcgcgcgcga cctcggcgtc gccggcgacg tgctgatcgc cggcttccac
      661 caggacgtgc gcccgctgct cgcggcgagc gacgtggtgg cgctgcccag cacctcggtg
      721 gagacgttct cgctcgccgc gctcgaagcg atggcgctcg gccggccggt ggtgctgtcg
      781 gagatcggcg gcgcggccga gatggtgcgc cccgacgaga acggctttct gtttcccgcc
      841 cacgacaccc cggcgctggt ggccgcgctc ggcgcgctcg ccgacccggc ggcgcgcgag
      901 cgcatgggcg ccgcggcgcg cagcaccgtg gaggcgcgct tctccgagcg cgcgatggtg
      961 gaccgctacg aaggattgct ccagcaactc gtcttgacaa ggaaccaacg tgacaaccta
     1021 cgaaaaagtg cagcagcgca tcagggctga gcgccgcacc tggctggtga ccggcgtcgc
     1081 cggcttcatc ggctccaacc tcgcggtgaa gctgctcgag ctcggccagg acgtggtcgg
     1141 gctggacaac ttctccaccg gcaagcgcga caacatcccc gccggcgtac gcttcatcga
     1201 gggcgacatc cgctcgctcg acgcctgccg cgaggcgtgc aagggcgtcg agcgcgtgct
     1261 gcaccaggcc gcgctcggca gcgtgccgcg ctcgatcgac cggccgctcg acacgcacga
     1321 caacaacgtc aacggcttcc tcaacatgct ggtggcggcg cgcgacgccg gcgtgccgcg
     1381 catggtgttc gccagctcga gcgcggtgtt cggcgacgac ccgatcctgc ccaaggtcga
     1441 ggagcgcatc gggcgcgcgg cctcgcccta cgggctgtcg aagcacgtga acgagctcta
     1501 cgccggcgtg ttcgccacct gctacggctt caagtggatc ggcctgcgct acttcaacgt
     1561 gttcgggccg cgccagagcc ccgacggccc ctacgcctcg gtgatcccgg cgtggatcgg
     1621 cgcgctgctg cgcggccaca ccgcctacat caacggcgac ggcagcgcgg cgcgcgactt
     1681 ctgccacatc gacaacgtgg tgcaggccaa cctgctgtcg gcgatgaccg aggacccggc
     1741 cgcgatcgac caggcctaca acgtcgcgca cggcgagatg accacgctca acgagctcta
     1801 cgaaatcatc cgcacgctgc tcgcgaagcg catgccgcac ctggcgagcg tgcgcgccgt
     1861 gcaccgcgag ccgcggcgcg gcgacctgct gctgtcgcgc gccgacaccg gcaaggccgc
     1921 gcgcctgctc ggctaccggc cgaccaaggg cgtgatggac ggcctggagg ccaccatgga
     1981 ctggtacctg gccaacctgc agccggcgcg cgaagagacc aaggaggtgg tcaatggctg
     2041 aagtcctgga gaaaatcgaa acccggagcg aggccccggc cgcgctcgcc acggtcgcgg
     2101 tcgtcggcct gggctacgtc ggcctgccgg tggcggtggc gttcggcaag cagcgccgca
     2161 ccatcggcta cgacctgtcc aagcgccggg tggagaacct gaagcacatg gtggacacca
     2221 ccggagaggt gtcgaccgcc gagctgatcg aggccaggca cctgaaggcg agcgcgcacg
     2281 ccgccgacct cgcgcacgcc gacttcatca tcgtcgcggt gccgacgccg atcaacgccg
     2341 cgcgccagcc cgacctgtc
//
LOCUS       DASRIE010000126        13349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2754042, whole genome shotgun sequence.
ACCESSION   DASRIE010000126 DASRIE010000000
VERSION     DASRIE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754042"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="purH"
                     /locus_tag="VFB53_05480"
     CDS             complement(<1..277)
                     /gene="purH"
                     /locus_tag="VFB53_05480"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671887.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN86221.1"
                     /translation="MSKLQRALLSVSDKSGIAAFARALAQLGVQILSTGGTAQLLEQE
                     GIPVTEVSAHTGFPEMLDGRVKTLHPKIHGGLLARRDLPAHMAALERQ"
     gene            complement(274..513)
                     /locus_tag="VFB53_05485"
     CDS             complement(274..513)
                     /locus_tag="VFB53_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN86222.1"
                     /translation="MSRGNELSDAVKRSLERYFKDMDGERPTAIYDMVLKHVEKPMIE
                     LVLGRTAGNLTHAATMLGIDRNTLRKKMLQLRIKG"
     gene            complement(510..1478)
                     /gene="dusB"
                     /locus_tag="VFB53_05490"
     CDS             complement(510..1478)
                     /gene="dusB"
                     /locus_tag="VFB53_05490"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZN86223.1"
                     /translation="MQIGAHQLANALFAAPMAGVTDRPFRVLARRYGAGLAVSEMVSS
                     RPELRGTRKSMLRLDHAGEPGPVSVQIAGAEPAMMAEAARHNVARGAQIIDINMGCPA
                     KKVCDKYAGSALLEDEALVARILEAVVAAVAVPVTLKIRTGPSPERRNAVRVARIAEA
                     SGVQLLAVHGRTRACGFAGHAEYDSIAAVKAAVRIPVLANGDTETPAEARAVLEHTGA
                     DGLMIGRAAFGRPWIFREIGHFLATGAELAPPAPAEVEAVVREHLEALYALYGEAQGL
                     RVARKHLGWYARRLPGGESFRQGVVRLEAAAAQRAAVNDYFARLAA"
     gene            complement(1499..2545)
                     /locus_tag="VFB53_05495"
     CDS             complement(1499..2545)
                     /locus_tag="VFB53_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN86224.1"
                     /translation="MPGADVVVHGAGPVGCTLALALQGARHRVRLVEPRPGALPAFRP
                     IALSQASRLILERVGAWGSFPATPIEAIHVSQAGAFGRTRLQAAEAGVPALGYVTQYA
                     DLVAALRSRVAIDGAAGESARLTVHAEGGAEDSRERRYGHDAVVARVAAEPRSQALAY
                     ERFTADGPLALLPLAGEWALVWSALPERAAALAAAPQEEFLRELQAAFGERAGRFSAV
                     HGRACVPLALRTRSSRIGERTVYIGNAAQALHPVAGQGLNLGLRDAWDLAQALHAADD
                     PGDERLLARYAARRRLDAAATIGVTELLARGFLGADPLARAARAAMLTSLDILPAARR
                     FFARRMIFGPSALP"
     gene            complement(2532..3851)
                     /locus_tag="VFB53_05500"
     CDS             complement(2532..3851)
                     /locus_tag="VFB53_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZN86225.1"
                     /translation="MAQDASLRAARRLRLAAAMPGGVAVIPTSPERARNRDTHYPYRF
                     DSYFYYLTGFREPESVLVIVADPGTAPRSILFCRERNPERETWDGFRHGPAGARERFG
                     FDEAQPIDALDEAMAALLADRAALWYPVGADAEWDARAMRWLNAVRANARSGVAAPQQ
                     LHDVRAPLDEMRLVKDAAELATMRRAAAISTGAHRRAMQATRPGRNEYEIEAELLYEF
                     RRHGAQFPAYWPIVAGGANACVLHYVANDAPLADGTLLLIDAGCELDGYAADITRSFP
                     VGGRYSGAQREVYELVLAAQAAAIAEVRAGKPWNAPHDAAVKVLAQGMLDLKLLEGSL
                     DAVLEKETYKRYYMHRTGHWLGLDVHDAGDYKRAGAWRALAPGNVLTVEPGLYIRAAA
                     DVPERLHDIGVRIEDDVLVAAGGCEVLTAAAPKAVAEIEALMRDAGR"
     gene            complement(3869..4549)
                     /locus_tag="VFB53_05505"
     CDS             complement(3869..4549)
                     /locus_tag="VFB53_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN86226.1"
                     /translation="MKAMILAAGRGERLRPLTDGLPKALAEAGGKPLIAWQLERLAAA
                     GFREVVINLAWHGEQIEARLGDGRAFGLRLAYSREGEALESAGGIAKARALLGGSPFA
                     LVNCDVYCDFDLGALRRRALGGALAHLVLAPNPAHHPAGDFSLAQGQAGNAPAPRYTY
                     TGIALIDPALVAPVRAGDKAQLAPLLRAAADAGRLSGELHRGLWRDIGTAERLAELDA
                     LLRARTGA"
     gene            complement(4546..5505)
                     /locus_tag="VFB53_05510"
     CDS             complement(4546..5505)
                     /locus_tag="VFB53_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN86227.1"
                     /translation="MDKRLAALTRWLDGPLAGERFSLAPASADASFRRYFRATLAGGR
                     SYVAMDAPPDREDCRPFLHVAQLLREAGLHAPEVHAQDLAQGFLLLEDLGRRTYLDAL
                     DDSNAGHLMRDAIGTLIRWQLATRPGALPPYDEALLRREMALFPEWYLGRHLGRKPDA
                     SLDGVYDALAKSALAQPAVYVHRDYMPRNLMLSEPNPGVLDFQDAVLGPVSYDIASLT
                     RDAFVSWEEAQVLDWTVRYWEGAKKAGLPVAADFAEFWRAFEWMGLQRHLKVLGIFAR
                     LTYRDGKPKFLADTPRFLGYARATARRYAALAPLARLLEALPA"
     gene            5533..7794
                     /locus_tag="VFB53_05515"
     CDS             5533..7794
                     /locus_tag="VFB53_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HZN86228.1"
                     /translation="MRFYLHKRRFSLISMRFFPALPPLLFVAALAQAQAPAPPPEKEP
                     ATIDAKSIQGISGLEVTAEGGVEFRQGETSIYADYLKYNREFGRLEAEGGVRLEQDSD
                     RFFGPRLRYDTTDHTGVFEEPTFLMRREQTARGNAAKLEFLGRNRLRLSRARFTTCEP
                     GNDDWYVEARELDLDYESEEGVARGARLRFLGTTILGTPYFSFPLENRRKSGVLAPQY
                     SQSTLRGVELGLPFYWNIAPEQDAVITPTVMSKRGEQLKTQYRYLGASYQGELRLEYM
                     PEDRELRRSRTGYSVQHEQHFTPRLLGRVDMNHVSDDRYFVDLYSQVRQVSTGNLQRD
                     GYLQYAGGIGATSLSLQGRVQRFQTLQDPLAPIVPPYHRVPQINFGLARNDLGGWLDA
                     AAPVEYVRFTHASLVEGERSAFNPVLSAPVLGPGYFLTPKFGARHARYELNRAAPGQP
                     LRQTLNIPWFSLDSGLVFERDASWFGERMSQTLEPRLYYVYAPYRDQSQVPLFDTALA
                     DFNYAQLFSENRFVGGDRFGDANQITWAVTSRLLNERGEERLRATLGQRYYYEDERVG
                     LTAATPLRNSSESDWLASAGGRFARHWTFDGTLQYNPRDSRAERYGVQLRYSPEIAKV
                     LNFSYRYGRDLLRQVDVSGQWPVSTGWYAIGRYNYSIRDDRVLEALGGMEYNGGCWVF
                     RAVFQRIQAATDVSSKVFMFQLEFNGFGQIGSNETVNLFRRNVPGYSVTNPANQSLVP
                     PSVRPPLPFEQVF"
     gene            7794..9077
                     /locus_tag="VFB53_05520"
     CDS             7794..9077
                     /locus_tag="VFB53_05520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN86229.1"
                     /translation="MRRALALLLLAAAPSGAALPATLVDRIVAVVNKEVITLTELADR
                     TAVAERELQRRRIAAPERAVLERQVLERLVLDKAQLQLAVDTGLRIDELQLDRAAQRV
                     AENNKMTLAEFRAVLERDGVAFERFRADLRQQMLLQRLREREVDGRVQVGESEVDLYL
                     EEHKASAALALEYEVAHILVRIPEQAGPERIEEARARAARARQQAQAGADFAQLAAGV
                     SEAGDALQGGALGWRTPGRLPELFADALVKMQPGEVSELLRSPAGFHVLKLLGRRGTG
                     AAAPVTQTRARHILVKTSEVVSEEDARQRLARLRERIAGGADFGELARLNSEDASAAR
                     GGDLGWIHPGDTVPEFERAMSALAPGELSQPVRTPFGFHLLQVQERRTADLSTERQRQ
                     QARQALHERKAEEAYQDWLRQLRDKTYVELRLEER"
     gene            9074..9976
                     /gene="pdxA"
                     /locus_tag="VFB53_05525"
     CDS             9074..9976
                     /gene="pdxA"
                     /locus_tag="VFB53_05525"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZN86230.1"
                     /translation="MKRIALTSGEPSGIGPELCRAVARRFRGAQLVAIGDAGLLEGCP
                     RVEHVPLAAPSRAGRLDPRNSRYVLAVLDRAIAGCRSGEYQAMVTAPVHKGAINDAGI
                     AFTGHTEYLAQRAGAPHVVMMLVGGGLRVALATTHLALKDVPRAITQAGLVATLQVIH
                     ADLKKRFGIKRPRILVSGLNPHSGESGHLGTEDRDVIEPALREAKKLGIAAQGPIPAD
                     TLFVPARLQGADCALAMFHDQGLPVLKYASFGRGVNVTLGLPFVRTSVDHGTALEIAG
                     SGKADPGSLLEALKLAVKLAPSRR"
     gene            9945..10730
                     /gene="rsmA"
                     /locus_tag="VFB53_05530"
     CDS             9945..10730
                     /gene="rsmA"
                     /locus_tag="VFB53_05530"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZN86231.1"
                     /translation="MPSNSHRPAVKRPHVARRRFGQHFLHDAGVVRRIVAAIAPAPED
                     CVVEIGPGEGVLTRPLRARAGRFEAIEIDRDLAAALAAEGVSVHVADALEFDFGVFPQ
                     GMRVVGNLPYNISTPLLFHLARHAARLRDLHVMLQREVVERMVARPSTPEYGRLSVML
                     QTRFRMEKLFRVAAGAFRPPPKVESAVVRLLPLAAPPDCDARRLEEVVRRAFSARRKT
                     LRNALALGAADFAALGLDPGLRPENLAPQDYVRIAQRTIGKAD"
     gene            10802..11326
                     /locus_tag="VFB53_05535"
     CDS             10802..11326
                     /locus_tag="VFB53_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZN86232.1"
                     /translation="MNTQGLVLAGLAAVALAGCQSMSSSDGPALASAGLMATKGNKTV
                     GEVTFEQVNGKVRVTAQVIGLKPGQEHGFHIHEVGDCSSGDGMSAKGHFNPHGKPHGR
                     GDVRHAGDLPNLNADKAGRAKMSVDLDILSVTPGPASVVGRGIIVHAQPDDYTSQPVG
                     NAGARMACGVIKAN"
     gene            complement(11352..11735)
                     /gene="gloA"
                     /locus_tag="VFB53_05540"
     CDS             complement(11352..11735)
                     /gene="gloA"
                     /locus_tag="VFB53_05540"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZN86233.1"
                     /translation="MRLLHTMLRVGNLERSVKFYTEVLGMKVLRTTDRPEQKYSLAFV
                     GYDAEDRTAVIELTYNYGVDRYDLGSAYGHIAIGVPDAAKACAAVRAQGGKVTREAGP
                     VKGGTTVIAFVEDPDGYKIEFIERR"
     gene            complement(11747..12448)
                     /locus_tag="VFB53_05545"
     CDS             complement(11747..12448)
                     /locus_tag="VFB53_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN86234.1"
                     /translation="MTLARSALFALALVLVTPPYALLALASFPLPRMARYRLISAWSR
                     LVIWLAWVICGIRWKIEGRKHLPDEPAVILAQHQSAWETLAFQEIFPPQVLVLKRELL
                     WIPFFGWGLALMSPIAIDRARGVAAMREMARRGRERLAQGFWVVVFPEGTRVAPGEKR
                     PYQQGGAWLAVHCGVPVVPVAHNAGRLWPRNAFLKRAGTVTVRIGAPIEAAGRTPKEV
                     LAEVRTWIEAQQATL"
     gene            complement(12445..12978)
                     /gene="gmhB"
                     /locus_tag="VFB53_05550"
     CDS             complement(12445..12978)
                     /gene="gmhB"
                     /locus_tag="VFB53_05550"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZN86235.1"
                     /translation="MKLVILDRDGTINHDSDQFIKSPAEWKPIKGSLEAIARLAQDGW
                     HVAVATNQSGIARGLFDMATLNAIHDAMHQAVHRAGGRIDAVFFCPHADASHCECRKP
                     RPGMLLEIARRMNAELAEVPMVGDALRDLQAAAAAGARPVLVLTGKGRQTRDAGGLPA
                     GTAVFADLAAFAAHLAP"
     gene            complement(12975..>13349)
                     /locus_tag="VFB53_05555"
     CDS             complement(12975..>13349)
                     /locus_tag="VFB53_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HZN86236.1"
                     /translation="AVRAFARLPEAPSLAAANKRIANILRQAGEVPGEFDAALFSQDE
                     EKALAGAFAAVQPGFEQAYKAGDYSAALQSLAALKAPVDAFFDKVLVMDPDEGLRRNR
                     IGFLGKLHATMNRIADLSKLAA"
BASE COUNT         1859 a         4806 c         4875 g         1809 t
ORIGIN      
        1 cctgccgctc gagcgccgcc atgtgcgcgg gcaggtcgcg ccgcgccagc agcccgccgt
       61 ggatcttcgg gtgcagcgtc ttcacgcgcc cgtcgagcat ttcgggaaag ccggtgtgcg
      121 cggagacctc ggtgaccggg atgccttcct gctccagcag ctgcgccgtg ccgccggtgg
      181 agaggatctg cacccccagc tgcgcgaggg cgcgggcgaa tgcggcgatg cccgacttgt
      241 ccgagacgct gaggagcgcc cgctggagct tgctcaccct ttgatccgca gctgcagcat
      301 cttcttgcgc agggtattgc ggtcgatgcc gagcatggtg gcggcatgcg tcaggttgcc
      361 cgcggtgcgg cccagcacca attcgatcat cggcttctcc acgtgcttga gcaccatgtc
      421 gtagatggcg gtcgggcgct cgccgtccat gtccttgaag tagcgctcga gcgagcgctt
      481 cacggcgtcg gacagctcgt tgccccggct catgcggcga gcctggcgaa atagtcgttg
      541 accgcggcgc gctgcgccgc cgccgcctcg aggcgcacca cgccctggcg gaagctctcg
      601 ccgccgggca ggcggcgcgc gtaccagccc aggtgcttgc gcgccacgcg caggccctgc
      661 gcctcgccgt acagcgcgta cagcgcctcg aggtgctcgc gcaccaccgc ctcgacctcg
      721 gccggcgccg gcggcgcgag ctcggcgccg gtggcaagga aatgcccgat ctcgcggaag
      781 atccagggcc ggccgaaggc ggcgcggccg atcatcaggc cgtcggcgcc ggtgtgctcg
      841 agcaccgcgc gcgcctcggc gggggtctcg gtgtcgccgt tggcgagcac cgggatgcgc
      901 accgccgcct tcaccgcggc gatgctgtcg tactccgcgt gaccggcgaa gccgcaggcg
      961 cgcgtgcggc cgtggacggc gagcagctgc acgcccgagg cctcggcgat gcgcgccacg
     1021 cgcaccgcgt tcctgcgctc cgggctcggc ccggtgcgga tcttgagggt caccggcacc
     1081 gccaccgcgg ccaccaccgc ctccaggatg cgcgccacca gcgcctcgtc ctccagcagc
     1141 gcggagccgg cgtacttgtc gcacaccttc ttcgccgggc agcccatgtt gatgtcgatg
     1201 atctgcgcgc cgcgcgccac gttgtgccgt gcggcctcgg ccatcatcgc cggctcggcg
     1261 ccggcgatct gcaccgacac cgggccgggc tcgccggcgt ggtccaggcg cagcatggac
     1321 ttgcgcgtgc cgcgcaactc gggccgcgac gacaccatct cggagaccgc gaggccggcg
     1381 ccgtagcgcc gcgcgaggac gcggaacggg cggtcggtga cccccgccat cggcgcggcg
     1441 aacaaggcat tggccaactg atgcgcgccg atctgcattg cccaattcta ttatttcttc
     1501 agggcagggc ggaaggtccg aagatcatgc gtcgcgcaaa aaagcgccgc gcggcgggca
     1561 ggatgtcaag actcgtcaac atcgcggcgc gcgcggcgcg cgcaagcggg tcggcgccca
     1621 ggaaaccgcg cgccagcagc tcggtgacgc cgatggtggc ggcggcgtcc agccgccgtc
     1681 gcgcggcgta gcgcgcgagc aggcgctcgt cgcccgggtc gtcggccgca tgcagggcct
     1741 gcgccaggtc ccaggcgtcg cgcaggccga ggttgaggcc ctggcccgcc accgggtgca
     1801 gcgcctgggc ggcattgccg atgtagaccg tgcgctcgcc gatgcgggaa gaacgcgtgc
     1861 gcagcgcgag cggcacgcag gcgcgcccgt ggaccgcgct gaagcgcccg gcgcgctcgc
     1921 cgaaggcggc ctgcaattcg cgcaggaact cctcctgcgg cgccgcggcg agcgccgcgg
     1981 cgcgttccgg cagcgcgctc cacaccagcg cccactcgcc ggcgagcggc agcagggcga
     2041 gcggcccgtc tgcggtgaag cgctcgtagg cgagcgcctg agagcgcggc tcggccgcca
     2101 cccgcgccac cacggcgtcg tggccgtagc ggcgctcgcg cgaatcctcg gctccgcctt
     2161 ccgcgtgcac cgtcagtcgc gcggactcgc cggcggcgcc gtcgatggcg acgcgagagc
     2221 gcagcgcagc cacaaggtcg gcgtattgcg tgacgtagcc gagcgccggc acgccggcct
     2281 cggcggcctg caggcgcgtg cggccgaagg cgccggcctg cgatacatgg atggcttcga
     2341 tcggcgtggc ggggaagctc ccccaggcgc cgacgcgttc caggatcagg cggctggcct
     2401 gcgacaacgc gatcggtcgg aacgcgggca gggcgccggg gcgcggctcg accagccgca
     2461 cgcgatgccg cgcgccctgc agcgcgagcg ccagcgtgca gccgaccggc cccgcgccgt
     2521 ggacgacgac gtcagcgccc ggcatcgcgc atcagcgcct cgatctcggc gaccgccttg
     2581 ggcgcggcgg cggtgagcac ttcgcagccg cccgcggcga ccagcacgtc gtcctcgatg
     2641 cgcacgccga tgtcgtgcag ccgctcgggc acgtccgccg cggcgcggat gtacagcccc
     2701 ggctccacgg tgagcacgtt gccgggggcg agcgcgcgcc aggcgcccgc gcgcttgtag
     2761 tcgcccgcgt cgtgcacgtc gaggcccagc cagtgcccgg tgcggtgcat gtagtagcgc
     2821 ttgtaggttt ccttctccag caccgcgtcg aggctgccct cgaggagctt aaggtcgagc
     2881 atgccctgcg ccagcacctt caccgcggcg tcgtgcggcg cgttccaggg cttgccggcg
     2941 cgcacctcgg cgatcgccgc ggcctgcgcg gcgagcacca gctcgtagac ctcgcgctgc
     3001 gcgccgctgt agcggccgcc gacggggaag ctgcgcgtga tgtcggcggc gtagccgtcc
     3061 agctcgcagc ccgcgtcgat caggagcagc gtaccgtcgg cgagcggcgc gtcgttggcc
     3121 acgtagtgca gcacgcaggc gttcgccccg cccgccacga tcggccagta ggcggggaac
     3181 tgcgcgccgt ggcggcggaa ctcgtacagg agttcggctt cgatctcgta ctcgttgcgc
     3241 ccggggcgcg tcgcctgcat ggcgcggcgg tgggcgccgg tggagatcgc cgcggcgcgg
     3301 cgcatcgtcg ccagctccgc ggcgtccttc accaggcgca tctcgtccag cggcgcgcgc
     3361 acgtcgtgca gctgctgcgg cgcggccacg ccgctgcgcg cattggcgcg cactgcgttc
     3421 agccagcgca tggcgcgcgc gtcccactcc gcgtccgcgc ccacggggta ccacagcgcg
     3481 gcgcggtcgg cgagcagcgc ggccatggcc tcgtccagcg cgtcgatcgg ctgcgcctcg
     3541 tcgaagccga agcgctcgcg cgcccccgcg ggcccgtggc ggaatccgtc ccaggtctcg
     3601 cgctccgggt tgcgctcgcg gcaaaacagg atcgagcgcg gcgccgtgcc ggggtcggcg
     3661 acgatcacca gcaccgactc gggctcgcga aagccggtga gatagtagaa gtagctgtcg
     3721 aagcgatagg gatagtgcgt gtcgcggttg cgtgcgcgct cgggcgacgt cgggatcacg
     3781 gcgacgccgc ccggcatcgc cgcggcgagc cgcagcctgc gcgcggcgcg cagcgacgcg
     3841 tcctgggcca gaccgtccgc tttcctgttc aagctcccgt ccttgcgcgc agcagcgcgt
     3901 cgagttccgc cagccgctcg gcggtgccga tgtcgcgcca caacccgcgg tgcagctcgc
     3961 cggagaggcg cccggcgtcg gcggccgcac gcagcagcgg cgcgagctgc gccttgtcgc
     4021 cggcgcgcac cggcgccacc agcgccgggt cgatgagcgc gataccggtg taagtgtagc
     4081 gcggcgcggg cgcgttgccg gcctggccct gcgcgagcga gaagtcgccc gccgggtggt
     4141 gcgccgggtt gggcgcgagc accaggtggg cgagcgcgcc gccgagcgcg cgacggcgca
     4201 gcgcgccgag gtcgaaatcg cagtacacgt cgcagttcac cagcgcgaag ggcgacccgc
     4261 cgagcagcgc gcgcgccttg gcgatgccgc ccgccgactc cagcgcctcg ccttcgcgcg
     4321 agtaggcaag gcgcaggccg aaagcgcgcc cgtcgcccag gcgcgcctcg atctgctcgc
     4381 cgtgccaggc gaggttgatc accacctcgc ggaagcccgc cgccgcgagg cgctcgagct
     4441 gccaggcgat gaggggcttg ccgccggcct cggcgagcgc cttgggcagg ccgtcggtga
     4501 gcgggcgcag ccgctcgccg cgcccggcgg cgaggatcat cgccttcatg ccggcagcgc
     4561 ctcgagcagg cgcgcgagcg gcgcgagcgc ggcgtagcgc cgggcggtgg cgcgcgcgta
     4621 gccgaggaag cgcggcgtgt cggcaaggaa cttcggcttg ccgtcgcggt aggtgaggcg
     4681 cgcgaagatg cccagcacct tcaggtggcg ctgcaggccc atccactcga aggcgcgcca
     4741 gaactcggcg aagtcggccg cgaccggcag gcccgccttc ttcgcgccct cccagtagcg
     4801 cacggtccag tcgagcacct gcgcctcctc ccagctgaca aaggcgtcgc gcgtgagcga
     4861 ggcgatgtcg tagctcaccg gcccgagcac cgcgtcctgg aagtcgagca cccccgggtt
     4921 gggctcggag agcatgaggt tgcgcggcat gtagtcgcgg tgcacgtaca ccgcgggctg
     4981 cgccaacgcg ctcttcgcca gcgcgtcgta gacgccgtcc aaagacgcat ccggcttgcg
     5041 tccgagatgc cgcccgaggt accactcggg aaacagcgcc atctcgcggc gcaggagcgc
     5101 ctcgtcgtag ggcggcagcg cgcccgggcg cgtggccagc tgccagcgga tcagggtgcc
     5161 gatggcgtcg cgcatcaggt gcccggcgtt gctgtcgtcc agcgcgtcga ggtaggtgcg
     5221 gcgcccgagg tcctcgagca gtaggaaccc ctgcgccagg tcctgcgcat gcacttcggg
     5281 ggcgtgcagg ccggcctcgc gcagcagctg cgccacgtgc aggaacggcc ggcagtcctc
     5341 ccggtcgggc ggcgcgtcca tggcgacgta gctgcgcccg ccggcgagcg tggcgcggaa
     5401 atagcggcgg aagctcgcgt cggccgaggc cggggccagg ctgaagcgct cgccggcgag
     5461 ggggccgtcg agccagcgcg tgagcgcagc gagccgtttg tccacagggt gcgttgcgct
     5521 cagcgtggta atgtgcgatt ttacctccac aagcgccgct tctccttgat ctccatgcgc
     5581 tttttcccgg cgctgccgcc gctgctgttc gtcgccgccc tggcgcaggc gcaggcgccg
     5641 gcgccgccgc cggaaaagga accggcgacc atcgacgcga agagcatcca gggcatctcc
     5701 ggcctggagg tgacggccga gggcggggtc gagttccgcc agggcgaaac ctcgatctat
     5761 gcggactacc tgaagtacaa ccgcgagttc ggccggctcg aggccgaggg cggcgtgcgc
     5821 ctggagcagg acagcgaccg cttcttcggg ccgcggctgc gctacgacac caccgaccat
     5881 accggcgtgt tcgaggagcc gaccttcctc atgcggcgcg agcagaccgc gcgcggcaac
     5941 gccgctaagc tggagttcct cggccgcaac cgcctgcggc tgtcgcgcgc gcgcttcacc
     6001 acctgcgaac cgggcaacga cgactggtac gtcgaggcgc gcgagctgga cctggactac
     6061 gagagcgaag agggcgtggc gcgcggcgcg cgcctgcgct ttctcggcac caccatcctc
     6121 ggcacgccgt atttcagctt cccgctggag aaccggcgca agagcggcgt cctcgcgccg
     6181 cagtactcgc agagcacgct gcgcggcgtc gagctcggcc tgccgttcta ctggaacatc
     6241 gcgcccgagc aggacgcggt gatcacgccc accgtgatgt ccaagcgcgg cgagcagctg
     6301 aagacgcagt accgctacct cggcgcgagc taccagggcg agctgcgcct ggagtacatg
     6361 cccgaggacc gcgagctgcg ccgctcgcgc accggctatt cggtccagca cgagcagcac
     6421 ttcacgccgc ggctgctcgg ccgcgtcgac atgaaccacg tgtcggacga ccgctatttc
     6481 gtcgacctgt acagccaggt gcgccaggtg tccaccggca acctgcagcg cgacggctac
     6541 ctgcagtacg cgggcggcat cggcgccacc tcgctcagcc tgcaggggcg cgtgcagcgc
     6601 ttccagaccc tgcaggaccc gctcgcgccc atcgtcccgc cctaccaccg cgtgccgcag
     6661 atcaatttcg ggctggcgcg caacgacctg ggcggctggc tggacgcggc cgcgccggtc
     6721 gagtacgtgc gcttcacgca cgcctcgctg gtcgagggcg agcgcagcgc gttcaacccg
     6781 gtgctcagcg cgccggtgct cggcccgggc tacttcctca ccccgaagtt cggcgcgcgc
     6841 cacgcgcgct acgagctgaa ccgcgccgcg ccgggccagc cgctgcgcca gacgctcaac
     6901 atcccctggt tcagcctgga cagcggcctg gtgttcgagc gcgacgccag ctggttcggc
     6961 gagcgcatgt cgcagaccct ggaaccgcgc ctgtactacg tctacgcgcc gtaccgcgac
     7021 cagtcgcagg tcccgctgtt cgacaccgcg ctcgcggact tcaattatgc gcagctgttt
     7081 tccgagaacc gcttcgtcgg cggcgaccgc ttcggcgacg ccaaccagat cacctgggcg
     7141 gtgacctcgc gcctgctcaa cgagcgcggc gaggagcggc tgcgcgccac cctcggccag
     7201 cgctactact acgaggacga acgcgtcggc ctcaccgccg ctacgccgct gcgcaactcg
     7261 agcgaatcgg actggctggc gtccgccggc ggccgcttcg cgcgccactg gaccttcgac
     7321 ggcacgctgc agtacaaccc gcgcgattcg cgcgccgagc gctacggcgt gcagctgcgc
     7381 tacagccccg agatcgccaa ggtgctcaat ttcagctacc gctacggccg cgacctgctg
     7441 cgccaggtgg acgtgtcggg ccagtggccg gtgagcacgg gctggtacgc catcggacgc
     7501 tacaattatt cgatccgcga cgatcgcgtg ctcgaggccc tggggggcat ggagtacaac
     7561 ggcggctgct gggtgttccg cgcggtgttc cagcgcatcc aggccgcgac cgacgtcagt
     7621 tccaaggtct tcatgttcca gctcgaattc aacggcttcg gccagatcgg ctccaacgag
     7681 acggtgaacc tgttccggcg caacgttcca ggctactcgg tgaccaatcc ggccaaccag
     7741 tcgctggtgc cgcccagcgt gcgcccgccg ctgccgttcg agcaggtgtt ctgatgcggc
     7801 gcgcgctggc cctgctgctg ctcgccgcgg cgccctccgg cgccgcgctg ccggcgacac
     7861 tggtcgaccg catcgtggcg gtggtgaaca aggaagtgat caccctgacc gagcttgccg
     7921 atcgcaccgc ggtcgccgag cgcgagctgc agcggcggcg catcgccgcg cccgagcgcg
     7981 cggtgctcga gcgccaggtg ctcgagcgcc tggtgctgga caaggcgcag ctgcagctgg
     8041 ccgtcgacac cgggctgcgc atcgacgagc tgcagctgga ccgcgccgcg cagcgcgtcg
     8101 ccgagaacaa caagatgacg ctggccgagt tccgcgccgt gctcgagcgc gacggcgtgg
     8161 cgttcgagcg cttccgcgcc gatctgcgcc agcagatgct gctgcagcgg ctgcgcgagc
     8221 gcgaggtcga cggccgcgtg caggtgggcg agagcgaggt cgacctgtac ctcgaggagc
     8281 acaaggcctc ggcggcgctc gccctggagt acgaggtggc gcacatcctg gtgcgcatcc
     8341 ccgagcaggc cggccccgag cgcatcgagg aggcgcgcgc gcgcgccgcg cgcgcgcggc
     8401 agcaggcgca ggcgggcgcc gacttcgcgc agctggcggc cggcgtgtcg gaggcgggcg
     8461 atgcgctgca gggcggcgcg ctcggctggc gcacgcccgg gcggctgccc gagctgttcg
     8521 ccgacgcgct ggtgaagatg cagccgggcg aagtgagcga gctgctgcgc agcccggcgg
     8581 gcttccacgt gctgaagctc ctcgggcggc gcggcaccgg cgccgcggcc ccggtgacgc
     8641 agacgcgcgc gcgccacatc ctggtgaaga ccagcgaagt ggtgtccgag gaagacgcgc
     8701 gccagcgcct cgcgcggctg cgcgagcgca tcgccggcgg ggcggatttc ggcgaactgg
     8761 cgcggctcaa ctccgaggac gcgagcgcgg cgcgcggcgg cgacctgggc tggatccatc
     8821 ccggggacac ggtgcccgaa ttcgagcgcg cgatgagcgc gctcgcgccg ggcgagctga
     8881 gccagccggt gcgcacgccc ttcggcttcc acctgctgca ggtgcaggag cggcgcaccg
     8941 ccgacctctc caccgagcgc cagcgccagc aggcgcgcca ggcgctgcac gagcggaagg
     9001 ccgaagaggc ctaccaggac tggctgcgcc agctgcgcga caagacctac gtcgagctgc
     9061 ggctggagga gcgttgaagc gcatcgcgct cacctcgggc gagccgtctg gcatcgggcc
     9121 ggagctgtgc cgcgcggtgg cgcggcgctt ccgcggcgcg cagctggtgg cgatcgggga
     9181 tgcgggcctg ctcgagggct gcccccgcgt cgagcacgtg ccgcttgccg cgccgagccg
     9241 ggccggccgg ctggatccgc gcaacagccg ctacgtgctc gcggtcctcg atcgcgcgat
     9301 cgccggctgc cgctcgggcg agtaccaggc catggtcacc gcgccggtgc acaagggcgc
     9361 gatcaacgac gccggcatcg ccttcaccgg gcacaccgag tacctggcgc aacgcgccgg
     9421 cgcgccgcac gtggtgatga tgctggtggg cggcgggctg cgcgtggcgc tcgccaccac
     9481 gcacctggcg ctcaaggacg tgccgcgcgc catcacgcag gccgggctgg tcgcgaccct
     9541 gcaggtgatc cacgccgacc tgaagaagcg cttcggcatc aagcggccgc gcatcctggt
     9601 gtcggggctc aatccccaca gcggggagtc cgggcacctg ggaacggaag accgggacgt
     9661 gatcgagccc gccctgcggg aggcgaaaaa gctgggcatc gcggcgcagg ggccgatccc
     9721 ggccgatacg ctgttcgtgc cggcgcggct gcagggcgcc gactgcgcgc tcgccatgtt
     9781 ccacgaccag ggcctgccgg tgctcaagta cgccagcttc gggcgcgggg tcaacgtcac
     9841 gctggggctg ccgttcgtgc gcacgtcggt ggaccacggc acggcgctgg agatcgcagg
     9901 ctcgggcaag gccgaccccg gcagcctgct cgaggcgctc aagcttgccg tcaaactcgc
     9961 accgtcccgc cgttaaacgg ccgcacgtag cgcggcgaag attcggccag cacttcctgc
    10021 acgacgcggg ggtggtgcgc aggatcgtcg ccgcgatcgc ccccgcgccg gaggattgcg
    10081 tcgtcgagat cgggccgggg gagggcgtgc tcacgcggcc gctgcgcgcg cgcgccgggc
    10141 ggttcgaggc gatcgagatc gaccgcgacc tcgccgcggc gctcgctgcc gagggcgtgt
    10201 cggtgcacgt cgccgacgcg ctggagttcg atttcggcgt gttcccgcag ggcatgcgcg
    10261 tggtcggcaa cctgccctac aacatctcca cgccgctgct gttccacctc gcgcgccacg
    10321 ccgcgcggct gcgcgacctg catgtcatgc tgcagcggga agtggtggag cgcatggtgg
    10381 cgcgcccctc cacgcccgag tacgggcgcc tctcggtgat gctgcagacg cgctttcgca
    10441 tggagaagct gttccgcgtc gccgccggcg cgttccggcc gccgcccaag gtggaatcgg
    10501 cggtggtgcg cctgctgccg ctcgccgcgc cgccggactg cgacgcgcgg cggctggagg
    10561 aggtggtgcg ccgggcattc agcgcgcggc gcaagaccct gcgcaatgcg ctcgcgctcg
    10621 gcgcggccga tttcgccgcg ctcggcctcg atccgggatt gcggccggag aacctggcac
    10681 cgcaggatta cgtgcgcatt gcgcagcgca ccatcggcaa agccgattga ggcggggcga
    10741 agcgcgcggt tacaatcggc gcaggatcga ccacaagaaa gatttcggag gaggacggca
    10801 aatgaacaca caggggctgg ttctggcggg gctcgcggcg gtcgcgctcg cgggctgcca
    10861 atcgatgtcg tcgtcggacg gtccggcgct cgcctcggcg ggactgatgg cgaccaaggg
    10921 caacaagacg gtcggcgagg tcactttcga gcaggtgaac ggcaaggtgc gcgtcaccgc
    10981 gcaggtcatc gggctgaagc ccggccagga gcacggtttt cacatccatg aggtgggcga
    11041 ctgcagctcg ggcgacggca tgagcgccaa gggccacttc aatccccacg gcaaaccgca
    11101 tggccgcggc gacgtgcgcc acgcgggcga cctgcccaac ctgaacgccg acaaggccgg
    11161 acgcgcgaag atgtccgtgg acctcgatat cctgagcgtg acgccggggc cggcaagcgt
    11221 cgtcggccgc ggcatcatcg tgcacgcgca gcccgacgac tacacctcgc agccggtggg
    11281 caatgccggc gcgcgcatgg cctgcggggt gatcaaggcg aactagttgc gggcgcgctc
    11341 gccgcgccgc actagcggcg ctcgataaac tcgatcttgt agccgtccgg atcctcgacg
    11401 aaggcgatca ccgtagtgcc gcccttcacc gggcccgcct cgcgcgtgac cttgccgccc
    11461 tgcgcccgca ccgcggcgca ggctttcgcc gcatccggca ccccgatcgc gatgtggccg
    11521 taggcggagc cgaggtcgta ccggtccacg ccgtagttgt aggtgagctc gatcaccgcg
    11581 gtccggtcct cggcgtcgta gccgacgaag gcgagcgaat atttctgctc gggccggtcg
    11641 gtggtgcgca gcaccttcat gccgagcacc tcggtgtaga acttgacgga gcgctcgagg
    11701 ttgccgacgc gcagcatggt gtgcaggagt ctcatggggc ggcagtctac aacgtcgcct
    11761 gctgggcctc gatccaggtc cgcacttcgg cgagcacttc cttgggcgtg cgcccggcgg
    11821 cctcgatcgg cgccccgatg cgcaccgtca cggtgccggc gcgcttgagg aaggcgttgc
    11881 gcggccacag gcgcccggcg ttgtgcgcca cgggcaccac cggcacgccg cagtgcaccg
    11941 ccagccaggc accgccctgc tggtaggggc gcttctcgcc cggcgccacg cgcgtgccct
    12001 cgggaaacac caccacccag aagccctgcg ccaggcgctc ccggccccgg cgcgccattt
    12061 cgcgcatcgc cgccacgccg cgcgcgcggt cgatggcgat cgggctcatc agcgcgaggc
    12121 cccagccgaa gaacgggatc cacaggagct cgcgcttcag caccagcacc tgcggcggga
    12181 agatttcctg gaaggcgagg gtttcccagg ccgactggtg ctgcgccagg atcaccgccg
    12241 gctcgtcggg caggtgcttg cggccctcga tcttccagcg gatgccgcag atcacccagg
    12301 cgagccagat caccagccgc gaccaggcgg agatcaggcg gtagcgcgcc atgcgcggca
    12361 gcggaaagct ggcgagcgcg agcagggcgt agggcggggt gacgaggacc agcgcgagcg
    12421 cgaacagcgc cgagcgcgcc agcgtcatgg ggccagatgc gcggcgaagg cggcgaggtc
    12481 ggcgaacacc gcggtccccg ccggcagccc gcccgcgtcg cgcgtctgcc ggcccttgcc
    12541 ggtcagcacc agcaccggcc tggcgcccgc ggcggcggcg gcctgcaggt cgcgcagcgc
    12601 gtcgcccacc atcggcacct ccgcgagttc ggcgttcatg cgccgcgcga tctcgagcag
    12661 catgcccggc ctgggcttcc tgcactcgca gtgcgaggcg tcggcgtgtg ggcagaagaa
    12721 taccgcgtcg atgcggccgc cggcgcggtg caccgcctgg tgcatggcgt cgtggatcgc
    12781 gttcagcgtc gccatgtcga acaggccgcg ggcgatcccc gactggttgg tggccaccgc
    12841 cacgtgccag ccgtcctgcg cgaggcgcgc gatcgcctcc aggctgccct tgatcggctt
    12901 ccattccgcc ggcgacttga tgaactggtc gctgtcgtgg ttgatggtgc cgtcgcggtc
    12961 caagatgacc agcttcatgc cgcgagcttg gacaggtcgg cgatgcgatt catggtggcg
    13021 tgcagcttgc cgaggaagcc gatgcggttg cggcgcaggc cctcgtccgg gtccatcacc
    13081 agcaccttgt cgaagaacgc gtccaccggc gccttcaggg cggcgaggga ctgcagcgcc
    13141 gcgctgtagt cgccggcctt gtaggcctgc tcgaagccgg gctgcacggc ggcgaaagcc
    13201 cccgccagcg ccttctcctc gtcctggctg aacagcgccg cgtcgaactc gccgggcacc
    13261 tcgcccgcct ggcgcaggat gttggcgatg cgcttgttgg cggcggcgag gctcggggct
    13321 tccggcaggc gcgcgaaggc gcgcaccgc
//
LOCUS       DASRIE010000127         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3252784, whole genome shotgun sequence.
ACCESSION   DASRIE010000127 DASRIE010000000
VERSION     DASRIE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3252784"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFB53_05560"
     CDS             <1..1087
                     /locus_tag="VFB53_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZN86237.1"
                     /translation="MNSGSIMDRLLRFCNALVGRWRGGLAQVNVLQSIIFASMSGSAL
                     ADAAGSGKIMQTLMTKDGKYTPSFAAALTAASSVIGPIIPPSIPLVLYALVSDTSIGY
                     LFLAGVIPGLLIGVAQSALIAYTARRRNFPVEAPVPLRDLPRVTWQAIPALLMPVILL
                     GCLYSGITTPTEAAAVAALYALLASAIIYRSIGWNDVYYSLMTSSRITISIGMLIAGA
                     LVFNYVITAENIPRTLSGYLKAYELSPLGFLLAANVLLLLLGCFLEGTTILLVIVPVL
                     LPTAAALGVDPVHFGVVAVVNIMIGLVTPPYGLLLFMMVKIANVTLRDLVREVMPFLA
                     VMIGALALITLVPDLVLFLPRLLGYKG"
     gene            1100..1696
                     /locus_tag="VFB53_05565"
     CDS             1100..1696
                     /locus_tag="VFB53_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86238.1"
                     /translation="MLGSAAVAMWWDVAPAMRAEWEHWHSSEHMPERLAIPGFLRGTR
                     WVARSGEPSYFVLYEVADLATITSGAYLERLNNPTPWSRKMMPHHRNMVRSLCAVRAG
                     WGGGVPQALATVRFSPASGLPKLPRGQGLTGAHLLESQPMPAAQTAEQKIRGGDASAD
                     WVLLVGGYDADALAPTPLPEGVVGFYRPAWSLSAGEIA"
     gene            1693..2229
                     /locus_tag="VFB53_05570"
     CDS             1693..2229
                     /locus_tag="VFB53_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN86239.1"
                     /translation="MKSVRRIVTGHRPDGRSTVLMDHAAANVKQRHAGNGSTLLWVTD
                     ETPARPSGEDRAAREIGVPPPRRGTIFRIAEFPPGVGGEVRDHETVLRDFGIGADVAR
                     GHAPRHPAIHRTRSVDYVVVLEGEIDLLLDDRDVHLKAGDVVVQQATNHAWINRGAQV
                     CRLAMVFVDAEEPAELKR"
     gene            2226..2969
                     /gene="aroD"
                     /locus_tag="VFB53_05575"
     CDS             2226..2969
                     /gene="aroD"
                     /locus_tag="VFB53_05575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HZN86240.1"
                     /translation="MKPIPIRGKPAAMPLVCAPLVGATREAVLAEAAAALAAKADIVE
                     WRVDHFRNFQDVMETGRALRRIGLPILFTRRSPAEGGNAVPISEKQVVELYGEVCADG
                     FADLVDYEMSNPAQDIKAVREVSRRHGVGLVCSFHDFERTPPLAELAARYRRAQELGG
                     DVAKVSVMARAPEDALTLLAATQQASQALALPLIGVSMGPHGALSRMIGFAFGSALTF
                     GVAAGRSAPGQMPIGELRAAIEIARKALG"
     gene            2962..>3312
                     /locus_tag="VFB53_05580"
     CDS             2962..>3312
                     /locus_tag="VFB53_05580"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656416.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN86241.1"
                     /translation="MARKLLVGLIGAGIQRSLSPALQEEEARHHGLRLHYQLIDLDPA
                     GLGNEALPGLISAVRIMGFAGLNITFPCKQAVIPLLDGLSDEARAIGAVNTVVREGDR
                     LVGYNTDGPGWSWGF"
BASE COUNT          465 a         1147 c         1167 g          533 t
ORIGIN      
        1 catgaacagc ggcagcatca tggaccggct gctgcgcttc tgtaacgcgc tggtcgggcg
       61 ctggcgggga ggcctggccc aggtcaacgt gctgcagagc atcatcttcg ccagcatgtc
      121 cggatcggcg ctcgccgacg cggcgggcag cggcaagatc atgcagacgc tgatgacgaa
      181 ggacggcaag tacacgccca gcttcgccgc cgcgctcacc gccgcctcgt cggtgatcgg
      241 gccgatcatc ccgccgtcga tcccgctggt gctctatgcg ctggtgtcgg acacctcgat
      301 cgggtatctg ttcctcgccg gcgtgattcc cggcctgctc atcggcgtgg cgcagagcgc
      361 gctgatcgcc tacacggcgc ggcgccggaa tttcccggtc gaggcgccgg tgccgctgcg
      421 cgatctgccg cgcgtcacgt ggcaggcgat tccggcgctg ctcatgccgg tgatcctgct
      481 cggctgcctg tacagcggca tcaccacgcc gaccgaagcc gcggccgtcg ccgcgctcta
      541 cgctttgctc gcttccgcga tcatctaccg cagcatcggc tggaacgacg tctactactc
      601 gctgatgacg agctcgcgca tcaccatctc catcggcatg ctgatcgccg gcgcgctggt
      661 cttcaactac gtgatcaccg ccgagaacat cccgcgcacg ctgagcggct acctgaaggc
      721 ctacgagctc tcgccgctcg gcttcctgct cgcggcgaac gtgctgctgc tgctcctcgg
      781 ctgcttcctc gagggcacca ccatcctgct ggtgatcgtg ccggtgctgc tgccgacggc
      841 cgcggcgctc ggggtcgatc cggtgcattt cggcgtggtg gcggtggtga acatcatgat
      901 cggtttggtc acgccgcctt acggcctgct gctcttcatg atggtgaaga tcgccaacgt
      961 gacgctgagg gacctggtgc gcgaggtgat gccgttcctc gcggtgatga tcggcgcgct
     1021 cgcgctgatc acgctggtcc ccgacctggt gctgttcctg ccgcgcttgc tgggctacaa
     1081 ggggtgaact gaggcttcca tgctgggtag cgcggccgtg gcgatgtggt gggacgtagc
     1141 gccggcgatg cgcgccgagt gggagcactg gcattccagc gagcacatgc ccgagcggct
     1201 ggcgatcccg ggattcctgc gcggcacgcg ctgggtggcg cgctcgggcg agccttccta
     1261 tttcgttctg tacgaggtcg ccgacctggc cacgatcacc tccggcgcgt acctcgagcg
     1321 gctcaacaac cccacgccgt ggtcgcgcaa gatgatgccg caccaccgca acatggtgcg
     1381 cagcctgtgc gcggtgcgcg cgggctgggg cggcggcgtg ccgcaggcgc tggcgacggt
     1441 gcgcttttcc ccggcgtccg ggctgcccaa gctgccgcga ggccagggcc tgaccggcgc
     1501 gcacctgctc gagtcgcagc cgatgcccgc ggcccagacc gccgagcaga agattcgcgg
     1561 cggcgacgcc agcgcggact gggtgctgct ggtcggcggc tacgacgcgg acgccctcgc
     1621 gccgacgccg ctgcccgagg gcgtggtcgg gttctatcgt ccggcctggt cgctgagcgc
     1681 gggagaaatc gcatgaagag cgtgaggcgc atcgtcaccg gccaccggcc ggatggccga
     1741 tcgaccgttc tcatggacca tgcggcggca aacgtgaagc aacggcatgc cggcaacgga
     1801 tcgaccctgc tctgggtcac cgacgagacg ccggcccgac cgtcgggcga ggaccgcgcc
     1861 gcgcgcgaga tcggcgtgcc gccgccgcgc cgcggcacca tcttccgcat cgccgagttt
     1921 ccgcccggcg tcggcggcga ggtgcgcgac cacgaaaccg tgctgcgcga cttcggcatc
     1981 ggcgccgacg tcgcgcgcgg ccatgcgccg cgccacccgg cgatccatcg cacgcgaagc
     2041 gtcgactacg tagtggtgct cgagggcgag atcgatctgc tgctcgacga tcgcgatgtg
     2101 catctcaagg ccggcgacgt agtggtgcag caggcgacga accacgcctg gatcaatcgc
     2161 ggcgcgcagg tatgtcgcct ggcgatggtg ttcgtcgatg ccgaagagcc ggcggagctg
     2221 aagcggtgaa gccgatccct atccgcggca agcccgccgc gatgccgctc gtctgcgcgc
     2281 cgctggtcgg cgcgacccgg gaggccgtgc tggcggaagc ggccgcggcc ttggcggcga
     2341 aggccgacat agtcgaatgg cgcgtggacc acttccggaa ttttcaggac gtgatggaaa
     2401 ccggtcgcgc attgcgccgc atcgggctgc cgatcctctt cacgcgccgc tcgccggcgg
     2461 aaggcgggaa cgccgtgcca atctccgaga agcaggtggt cgagctgtat ggcgaagtgt
     2521 gcgccgacgg tttcgcggac ctggtcgatt acgaaatgag caatcccgcc caggacatca
     2581 aggcggtgcg cgaggtatcc cgccggcacg gcgtgggcct cgtctgctcg ttccacgact
     2641 tcgagcgcac gccgccgctc gccgagctcg ccgcgcggta ccgccgcgcg caggagctgg
     2701 gcggcgacgt ggcgaaggtc tcggtgatgg cgcgcgcgcc ggaggacgcg ttgacgctgc
     2761 tcgccgccac gcagcaggcc tcgcaggcgc tcgcgctgcc gctgatcggc gtgtccatgg
     2821 ggccgcacgg cgcgctcagc cgcatgatcg gcttcgcctt cggctcggcg ctcaccttcg
     2881 gcgtcgccgc cggccgctcg gcgccggggc agatgccgat cggcgagctg cgcgccgcga
     2941 tcgagatcgc gagaaaagcc cttggctagg aagctcctcg tcggcctgat cggcgccggc
     3001 atccagcgct cgctctcgcc cgcgctgcag gaagaggagg cgcgccacca cggcctgcgc
     3061 ctgcattacc agctgatcga cctcgacccc gccggcctgg gcaacgaagc gctgcccggc
     3121 ctgatctcgg cggtgcgcat catgggattc gcggggctga acatcacctt tccgtgcaag
     3181 caggcggtga tcccgctgct cgacggcctg tccgacgagg cgcgcgcgat cggcgcggtc
     3241 aacaccgtgg tgcgcgaggg cgaccggctg gtcggctaca acaccgacgg tcccggctgg
     3301 agctggggct tc
//
LOCUS       DASRIE010000128         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1448542, whole genome shotgun sequence.
ACCESSION   DASRIE010000128 DASRIE010000000
VERSION     DASRIE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448542"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2551)
                     /locus_tag="VFB53_05585"
     CDS             complement(<1..2551)
                     /locus_tag="VFB53_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZN86242.1"
                     /translation="MHGSGTPDTDIARTLTGVLPASRVVTDPLRRLAYGTDASFYRLT
                     PEVVAVVESEAELQAVLRAARAHGRPVTFRAAGTSLSGQAVTHGVLVLIGDGFATCEI
                     GPGARAVRLGPGMVGADVNARLAPHGRKIGPDPASIATCKIGGIAANNASGMCCGTAQ
                     NSYHTMESLRVVLADGTVLDTEDAASVAAFRVSHAALLGELDRMAAETRADTALAGRI
                     RHKYRIKNTTGYSINALVDFADPVDILAHLMIGSEGTLGFISRITYRTVADDPCKASA
                     LVFFPTLGDACQAVLGLRQQPVAAVELLDRASLRSVQDKPGMPDVMRSLGVDAAALLV
                     EVRARTAPALQLRMDAALGVLAGLPTVEAAAFSTDPQVCESYWKVRKGTFPSVGAMRR
                     TGTTVIIEDVAFPVESLAAATLDLQAAMRRHGYDEGIIFGHALEGNLHFVFTQDFSDA
                     AEVARYARFMEDVCTLVVDTYDGSLKAEHGTGRNMAPFVEREWGRQATQLMRRIKALL
                     DPGTLLNPGVILDDDPHAHLKHLKPMPAAESMVDRCIECGFCEPMCPSHGLTLSPRQR
                     ITTWRERSRREAAGEATAEIDDRFGHLGVDTCAGCSLCSTACPVGIDTGGLVRLLRDR
                     RATPVARSLSEWSADHFDTVAALSRTGLRLGHAAARVVGDGVVSRLSGGAWKRGMPHA
                     AHRVAVPRSNAGDPVVYYPTCGGRIFGDSTPGQPSLHEVVTTLLERAGYAVRLPEGVE
                     RLCCGQMLFSKGRTQEADAMADTLAAALRAAADDGRGGFHPVVMDASACALRMKERLA
                     GRLQVLDFHEFAHDALLPRLRIRRGTAPVALHVNCSVRRSASDEKLRSVLRR"
     gene            2771..3517
                     /locus_tag="VFB53_05590"
     CDS             2771..3517
                     /locus_tag="VFB53_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN86243.1"
                     /translation="MSDAVAATIEQRILEGTLKPGDRLPSERELAAELGVSRPSLREA
                     IQKLVTKGLLHTRQRGGTQVTDRLDAHFTDPWEQMLSGHPDVHRDMLEFRQLLESQAA
                     YWAADRCTDVDIRMLDEAHTVLQAQYDGAPLDDCIDADLRFHQAIAEASHNVLVSHLC
                     ASLMKVVHGHIEDNLRALHPRPHRWDQLRLQHRDIWLAIREHRPEDAARAARAHIEFV
                     RQCMEDNALAQERRAVALRRSAQTPQPPRP"
     gene            3577..>4154
                     /locus_tag="VFB53_05595"
     CDS             3577..>4154
                     /locus_tag="VFB53_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HZN86244.1"
                     /translation="MARRGLFISYSHADRQWLRELHIQLVPLVRLGLALWDDTQLGPG
                     AQWRREIQRAIAQSRAALLLVSPDFLASEFIDRWELAPLLEARDKEALAILWVHVRAC
                     LYEQTAIGSLQAAHDVTRPLDSLAPPERGAMLAEICRRVQEAMGIGRGEPGTHGALAS
                     AAASGTAFIRYALVLSGTIVENDRPVADALLG"
BASE COUNT          609 a         1484 c         1488 g          573 t
ORIGIN      
        1 agcggcgcag cacgcttcgc agcttctcgt cggaggccga gcggcgcacg ctgcagttca
       61 cgtgcagcgc caccggggcg gtgccgcgcc ggatgcgcag gcgcggtagc agcgcatcgt
      121 gcgcgaactc gtggaagtcg agcacctgca ggcggcccgc caggcgctcc ttcatgcgca
      181 gggcgcacgc gctggcgtcc atcaccaccg ggtggaagcc gccccggccg tcgtcggcgg
      241 cggcgcgcag cgccgcggcg agcgtatcgg ccatcgcgtc ggcctcctgc gtgcggccct
      301 tgctgaagag catctggccg cagcacaggc gctcgacgcc ttcgggcagg cgcaccgcgt
      361 agcccgcgcg ctcgagcagc gtcgtgacga cctcgtgcag cgacggctgc cccggcgtgc
      421 tgtcgccgaa gatgcggccg ccgcacgtcg ggtaatagac caccgggtcg ccggcattcg
      481 agcgcggcac ggcgacgcga tgcgccgcgt gcggcatgcc gcgcttccac gcgccgcccg
      541 acaggcgcga caccacgccg tcgccgacca cgcgcgccgc ggcgtggccg aggcgcagcc
      601 ccgtgcgcga cagtgcggcc accgtgtcga agtggtcggc ggaccattcc gacaggctgc
      661 gcgccacggg ggtcgcgcgc cggtcgcgca gcaggcggac cagcccgccc gtgtcgatgc
      721 cgacggggca ggcggtggag caaaggctgc agcccgcgca cgtgtcgacg cccaggtggc
      781 cgaagcggtc gtcgatctcg gcggtcgcct cgccggccgc ctcgcgccgc gagcgctcgc
      841 gccacgtggt gatgcgctgg cgcggcgaga gcgtgagccc gtgcgacggg cacatcggct
      901 cgcagaagcc gcattcgatg cagcggtcga ccatcgactc ggccgccggc atcggcttca
      961 ggtgcttcag gtgggcgtgc ggatcgtcgt cgaggatgac gcccgggttc agcagcgtcc
     1021 cgggatcgag cagcgccttg atgcgccgca tcagctgcgt cgcctgccgc ccccactcgc
     1081 gctcgacgaa cggcgccatg ttgcggccgg tgccgtgctc ggccttgagc gagccgtcgt
     1141 aggtgtccac taccagcgtg cacacgtctt ccatgaagcg ggcgtagcgc gccacctcgg
     1201 ccgcgtcgga gaagtcctgc gtgaagacga agtggagatt gccctccagc gcgtggccga
     1261 agatgatccc ttcgtcgtag ccgtggcggc gcatcgcggc ctgcaggtcg agcgtcgcgg
     1321 cggcgagcga ctccaccggg aacgccacgt cctcgatgat caccgtggtg ccggtgcggc
     1381 gcatcgcacc caccgagggg aacgtgccct tgcgcacctt ccagtagctc tcgcacacct
     1441 ggggatcggt ggagaacgcg gccgcctcga cggtcggcag cccggcgagc acgcccagcg
     1501 ccgcgtccat gcgcaactgc agcgcgggcg ccgtccgcgc gcggacttcg acgagcagcg
     1561 ccgccgcgtc gacgcccagc gagcgcatca cgtccggcat gccgggcttg tcctgcaccg
     1621 agcgcaacga cgcgcggtcc agcagctcga cggcggccac cggctgctgc cgcaggccca
     1681 gcacggcctg gcaggcatcc ccgagggtcg ggaagaagac cagggcgctg gccttgcacg
     1741 ggtcgtcggc gaccgtgcgg taggtgatgc gcgagatgaa cccgagcgtg ccctccgagc
     1801 cgatcatcag gtgcgcgagg atgtccaccg ggtccgcgaa gtcgaccagc gcgttgatgc
     1861 tgtagccggt ggtgttcttg atccggtact tgtggcggat gcggccggcg agcgcggtgt
     1921 cggcgcgcgt ctcggcggcc atgcggtcga gctcccccag cagcgcggca tggctgaccc
     1981 ggaacgccgc gacgctcgcc gcgtcctcgg tgtccagcac ggtgccatcc gcgagcacca
     2041 cgcgcagcga ctccatcgtg tggtagctgt tctgcgccgt gccgcagcac atgccggagg
     2101 cgttgttggc cgcgatcccg ccgatcttgc aggtggcgat ggatgccggg tcgggaccga
     2161 tcttgcggcc gtgcggcgcc agccgggcat tcacgtcggc gcccaccatg ccgggaccga
     2221 gccgcaccgc gcgcgcgccg ggcccgatct cgcaggtcgc gaagccgtcg ccgatcagca
     2281 ccagcacgcc gtgcgtcacc gcctggcccg acaggctggt tcccgcggcg cgaaaggtca
     2341 cgggccggcc atgggcgcgg gcggcgcgca gcacggcctg cagctcggct tcggactcga
     2401 ccaccgccac cacctccggc gtgagccgat agaagctggc gtcggtgccg taggccaggc
     2461 gccgcagcgg atcggtgacg acccgactcg cggggaggac cccggtcagc gtccgggcga
     2521 tgtcggtgtc cggggttcct gagccgtgca tctcgtctcc ttgccttctg gtaagaccac
     2581 gtttccgaga tattctccag agaatcctga acgaaagctt tcacggctgg acagacaagc
     2641 gctcagtctg agaaaacgca agtttctacc actggtcagg ccagatttga ctgttcgatc
     2701 gcttgtgcta cagtggtcag accaggaggc aagagacatg tccacgaacc atccagaccg
     2761 agcgcggcgg gtgtccgatg cggtcgcggc gaccatcgag cagcgcatcc tcgaaggcac
     2821 cctgaagccg ggcgatcgcc tgccgtccga gcgggagctg gcggcggagc tcggcgtttc
     2881 gcggccgtcg ctgcgcgagg cgatccagaa gctggtgacc aaggggctgc tgcacacgcg
     2941 gcagcgcggc ggcacgcagg tcaccgaccg gctggatgcc cacttcaccg atccgtggga
     3001 gcagatgctc tccggccatc ccgacgtgca ccgcgacatg ctggagttcc gccagctgct
     3061 ggagtcgcag gcggcctatt gggcggcgga ccgctgcacc gacgtcgaca tccggatgct
     3121 ggatgaagcg cacacggtcc tgcaggcgca gtacgacggc gctccgctcg acgactgcat
     3181 cgacgccgac ctgcgcttcc accaggccat cgccgaggcc tcgcacaacg tgctcgtgag
     3241 ccatctctgc gcgagcctga tgaaggtcgt ccacggccac atcgaggaca acctgcgcgc
     3301 gctgcacccg aggccgcatc ggtgggatca gctgcggctg cagcatcgcg acatctggct
     3361 ggcgatccgc gagcaccggc cggaggacgc ggcacgtgcg gcacgcgccc acatcgagtt
     3421 cgtgcgccag tgcatggagg acaacgcgct cgcgcaggag cggcgcgcgg tggcgctgcg
     3481 tcgcagcgcc cagacgcccc agccgccgcg gccctgaatc accggcgggc ggccggggca
     3541 caatcgcgcc aggtcgactc acggcccaag gacacgatgg cacgcagggg cctgttcatc
     3601 agctacagcc atgccgaccg gcagtggctg cgcgagctgc acatccagct cgtccccctg
     3661 gtgcggctcg gcctggccct gtgggacgac acgcagctcg ggcctggtgc gcagtggcga
     3721 cgcgagattc aacgcgcgat cgcgcaaagc cgggcggccc tgttgctcgt gagccccgat
     3781 ttcctcgcct cggagttcat cgaccgctgg gagctcgcgc cgctgctcga ggcgcgcgac
     3841 aaggaagccc tcgccatcct gtgggtgcac gtgcgggcgt gcctgtacga gcagaccgcg
     3901 atcggctcgc tgcaggcggc ccacgacgtc acgcgccccc tggactcgct ggcgccgccg
     3961 gagcgcggtg ccatgctggc cgagatctgt cgtcgcgtgc aggaggccat gggcatcggg
     4021 cgcggcgagc ccggcacgca cggggcgctc gcctcggcgg ctgcgtccgg caccgccttc
     4081 atccgctacg cgctggtgct gagcggcacg atcgtcgaga acgaccggcc cgtggcggac
     4141 gccctgctcg gaca
//
LOCUS       DASRIE010000129        10317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2206179, whole genome shotgun sequence.
ACCESSION   DASRIE010000129 DASRIE010000000
VERSION     DASRIE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206179"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFB53_05600"
     CDS             <1..643
                     /locus_tag="VFB53_05600"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZN86245.1"
                     /translation="GPEGVSKELVAAWDPFHPVVEELRAVRTQLLVRWFNPGGGRRTL
                     VVTSPCAREGRSFIAANLAVVFSQLGQRTLLIDADFRAPRQQGIFNISDRFGLSSVLS
                     GRSDLSAAAPVAGITGLAVLPAGPLPPNPLELLSRGAFAALLARAQAEFDMIVIDTPP
                     VTEYADASAVTFRAGDALMISRKDHTRLVDTERAMRELGDASARVVGTLMNAF"
     gene            651..782
                     /locus_tag="VFB53_05605"
     CDS             651..782
                     /locus_tag="VFB53_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86246.1"
                     /translation="MKKPKLLLAALVLLIGAASAAGLKSPPGSNDMIYPKLFARAAP"
     gene            779..2218
                     /locus_tag="VFB53_05610"
     CDS             779..2218
                     /locus_tag="VFB53_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN86247.1"
                     /translation="MTPASSSLRIDVSGAARALAAMAALAGLAVLGGFALAVGEIQAL
                     YVGLSLVACVAILIDYRAGAILLVLLLPISSTALFPHALMGMTGLNPMNLLLMATLAS
                     FLARGHVGHPGPFVPRQVLWLLVVPIALAGLLGTTHFDDILPAFFDRMVVAFTTWRGY
                     LGVTVVKPVLLVLAALLVAAAVAKARKPESFVVALAAGACALALLMIGFVAISDIRLG
                     MLASPRARMFFSEIGAHANDFGRVFVTGYALLLFTWWESKDARARLALFVALGILSLG
                     ILLTFSRNAYLGFFLVNGLFLLWKFNMKKLGLALLGTAVAAALAPGAVYRRITYGFDS
                     GSADTVSAGRIEGLWLPLLPETLKSPLWGQGLDSIMWSDTMALGAMEMAGHPHNAYLQ
                     AVLDMGVIGLVLLLAYYWHVWRNFRALGSNPYLTPPMRGFFQGACAALLAFAAACMTG
                     GSLRPQADNVLLWIAIGIMYGLLVRKPKS"
     gene            2276..3376
                     /locus_tag="VFB53_05615"
     CDS             2276..3376
                     /locus_tag="VFB53_05615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86248.1"
                     /translation="MLSRKRVCFVAPRAWPVLSGDAAIPIVGGAEVQQSLLARLLAAH
                     GHRVSMICLDYGQPQRARVDGIEVIKTFGMTQGLPMLRFLHPRVTSMWRAMREVDAEI
                     YYFRSASMWLGVLAEFCRRHGRRSIYAGASNKDFVADVGGQLPYLRDRWLYRRGLAQA
                     DRIVAQNEAQRASLRATHGRDAVVIPSCYQAPARRGAAANPAARDRVLWVGTVHENKR
                     PELFLELAARLPHRRFVLIGGPRPGGEAFYERIRAQAAAAPNVEATGFLPLAEVEPWF
                     DRARVLVNTSTYEGMPNTFLQAWARGVPTVATVDVGAPVHQVVPNPEDLPGRVEAAFD
                     NPALGARCREYFERNHSPAGVLARYEELFASL"
     gene            3373..4809
                     /locus_tag="VFB53_05620"
     CDS             3373..4809
                     /locus_tag="VFB53_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZN86249.1"
                     /translation="MNTAAKAISWNGLRRRAFGLGAVKTFDHATHFLLPVVLTRCLDA
                     ATFGEYRLLWLIVGTLMTVATLNMAGGLFFFVPRSDARGKRLYIHQTMLFLAASGLVC
                     GALSGPWNPLLPAAVAPVAQYGWLVPAFVALWVAAILLDFLPTIDERIRWQAFATGTT
                     SVLRVALVGAAAWFTGELRAVLWMLLAFVLVKLALLLYYVHRHHGLGRPWFERAAFGA
                     QFSHCAPFGVSNALYSLRAQTDQWVAASLFALTSFAAFSVAAIVGHVVQILRHSVMEA
                     FMPTMSRMQAAGDVRGMMAMNSRANAMVGTMLYPLLALAFAFAEDIITLVYTAAYLEA
                     APVMRVYVVGMLAMVVEVGSIVLLLRQGPFALRITALVLPVSVAVSWLAAHELGLAGA
                     AAGSVVAIYLDRALLLRRVARHTGIALRELQAWRALGRTLALAAACGLLAWFLVQQFL
                     GGAAPLVRLAAGATILALAYVPLWRATR"
     gene            4806..5957
                     /locus_tag="VFB53_05625"
     CDS             4806..5957
                     /locus_tag="VFB53_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86250.1"
                     /translation="MKLLFVTGSLVHGGAERHTIGLLNRLAQRGHECHAAYVRDDASQ
                     LDRLQGAATVECLRAGGYLDAAALRRLGALIRRLAPSAVVAANPYAMMHAALALRQSG
                     VRAPLAVTFHTTLLPGLKEQLQMLYYRPLFWSADALVFVCEAQRRHWRRRLVFGRASE
                     VIHNGIDPEYWRPPAPGVGARVRRVLGFEDGDYVIGMCAVFRPEKNHKQLVEALAQLR
                     ARGVPARALLIGDGPLRGAIEARARALGVAGDVIIAGFQQDVRPLLAASDAVALCSTS
                     ETFSLAALEAMALGRPVILPEVGGAAEMVRHGRDGFLFPVGDTAALVSRLAALADAGL
                     RRRFGAGARESVEARFTERAMVDRYERLLQRIVLKRSQRENLRRSATAH"
     gene            5923..6951
                     /locus_tag="VFB53_05630"
     CDS             5923..6951
                     /locus_tag="VFB53_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86251.1"
                     /translation="MRTYEEVQQLIRSRRRTWLVTGAAGFIGSNLVEKLLSLEQDVVG
                     LDNFSTGKRENLQPGMRLLEGDIRSLDDCRAAVKGVDVVLHQAALGSVPRSIERPLDT
                     HDNNVNGFLNMLVATRDAGIPRMVFASSSAVFGDDPQLPKVEERIGQAASPYGLSKHI
                     NELYAQVFAACYGFEWVGLRYFNVFGPRQDADGAYASVIPAWIGALLRGESAYINGDG
                     SCARDFCHVDNVVQMNLRAATTEDPAALNQPYNVAHGEITSLTELFETIRTLLGKRLA
                     HLKGVRAVYREPRRGDIQLSRADIAKAQRLLGYEPTMRLMDGLEKTMDWYVANLAPRE
                     ERKVAHAA"
     gene            6941..8263
                     /locus_tag="VFB53_05635"
     CDS             6941..8263
                     /locus_tag="VFB53_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZN86252.1"
                     /translation="MLLEAQAPLREEQKLATVAVLGLGYVGLPVAVAFGRQRPTIGYD
                     ISRKRVENLKHRVDVTGELSAAELAEARNWHPTSDPAELGKADFIIVAVPTPINDARQ
                     PDLRPLQSASETVGRHMKAGATVIYESTVYPGCTEEVCVPILERSSGMRWRQDFHVGY
                     SPERINPGDKEHVFEKIRKIVSGDDAATLEAVAQLYGSVVKAGVYRASSIRVAEAAKV
                     IENTQRDLNIAFVNELAIIFEKLGIDTLEVLKAAGTKWNFLPFRPGLVGGHCIGVDPY
                     YLTYRAELAGYHPEVILAGRRINDAMGSHIARKTVQQMIHAGRNIKGARVNVLGLTFK
                     ENVPDIRNSKVIDIIRELHEFGVETFIHDPMAGADEALHEYGVRLAEWDSLPAADALI
                     LAVPHQQFLRRTAAELMEKIVRGGCVIDVKAVLDAQPFQREGLRVWRL"
     gene            8251..9693
                     /locus_tag="VFB53_05640"
     CDS             8251..9693
                     /locus_tag="VFB53_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86253.1"
                     /translation="MALVARASAARPARLRVGVFADSRLQPRWIVQALDRVARCGWAE
                     VVAVVAPQGVFPKNPWWARLDRWAFGSDLCEPVRLAAADSADRVALDLDVAFALGELD
                     DRALDGIARLGVWRIRADGAREVANQEPVSGAAITVRLAAGEAPRLAWESWGRTYPLS
                     VARNRRELFERAVELPARALRDAQRYGGAWLEQRPFAKADLPQAAPSLAAVSKRILQR
                     GVEKALYQEQWSLAWRFGALAPDLEGFTRLVPPPGRNWAHPFALARGGRYYVFFEDGG
                     RIAMLQVEEGGAWSAARTVLERDYPLSHPFLLEHEDALYMVPQTADNGTVELYRCVDF
                     PFLWRLERVLLEGVRLANPTVHRGVDRWWIFAGVGEHELHLFHAPRLSGGEWQPHARN
                     PVKSDVRSSRPAGAPYWRNGALYRPAQINAPRHGAGVALNRVLRLSAGEYAERQVERI
                     LPAGDLLGMQTVNRAGALTVIDFLTRRNRV"
     gene            9754..>10317
                     /locus_tag="VFB53_05645"
     CDS             9754..>10317
                     /locus_tag="VFB53_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86254.1"
                     /translation="MSSYSRLVMLGAAPETRGSIAAVVQAYRAHGLFKRWQIEYLPTH
                     SDRGFGHNAGLAVSAVQRFSSLLARHGRMPVHLHMAARGSALRDAAFIGAALAARCPI
                     LVQLHGAGFERSHGGPLIARMLERASFVVIPCESMRAWVRSVARHAHTVCLPTPVWCE
                     LQPSSRPNLVLFLGRMEASKGIYDLLQA"
BASE COUNT         1333 a         3712 c         3665 g         1607 t
ORIGIN      
        1 cggcccggaa ggcgtgagca aggagctggt cgccgcgtgg gacccgttcc acccggtggt
       61 cgaggagctg cgcgcggtgc gcactcagct cctggtgcgc tggttcaacc ccggcggcgg
      121 tcgccgcacg ctcgtcgtca cgagcccctg cgcgcgggag ggccgcagct tcatcgccgc
      181 gaacctcgcg gtggtgttct cgcagctcgg ccagcgcacg ctgctgatcg acgccgactt
      241 ccgcgcgccg cgccagcagg gcatcttcaa catctcggac cgcttcggcc tgtcctcggt
      301 gctctcgggg cgctcggacc tgtcggccgc cgcgccggtc gccgggatca ccgggctcgc
      361 ggtgctgccg gcgggcccgc tgccgcccaa cccgctcgag ctcttgtcgc gcggcgcctt
      421 cgccgcgctg ctcgcccggg cgcaggccga gttcgacatg atcgtgatcg acacgccgcc
      481 ggtcaccgag tacgccgacg ccagcgccgt caccttcagg gcgggcgacg cgctgatgat
      541 ctcgcgcaag gaccatacgc gcctcgtcga cacggagcgc gcgatgcgcg agctgggcga
      601 cgccagcgcg cgcgtggtcg gcacgctgat gaacgcgttc tagcgagagc gtgaagaaac
      661 cgaagctcct gctcgccgcg ctcgtgctcc tcatcggggc ggcgagcgcg gcggggctga
      721 aatcgccgcc gggatcgaac gacatgatct accccaagct cttcgcgcgg gccgcgccgt
      781 gacgcccgcg tcctcgtccc ttcgcatcga cgtctccggc gcggcgcgcg cgctcgccgc
      841 gatggcggcg ctcgccggcc tggcggtgct gggcggcttc gccctcgcgg tgggcgagat
      901 ccaggccctc tacgtcggcc tgtcgctcgt cgcctgcgtg gcgatcctga tcgactaccg
      961 cgcgggcgcg atcctgctcg tgctgctgct gccgatctcg tcgaccgcgc tcttcccgca
     1021 cgccctgatg ggcatgacgg gcctcaaccc catgaacctg ctgctcatgg ccacgctcgc
     1081 ctccttcctg gcgcgcgggc atgtcgggca ccccgggccg ttcgtgccgc ggcaggtgct
     1141 gtggctgctg gtggtgccga tcgcgctcgc cgggctgctg ggcaccacgc acttcgacga
     1201 catcctgccg gcgttcttcg accggatggt ggtcgcgttc accacctggc ggggctacct
     1261 cggcgtcacc gtggtgaagc cggtgctgct ggtgctggcg gcgctgctgg tggccgcggc
     1321 cgtggcgaag gcgcgcaagc ccgagagctt cgtggtggcg ctcgccgccg gcgcttgcgc
     1381 gctcgcgctg ctgatgatcg gcttcgtcgc gatctccgac atccgcctcg gcatgctcgc
     1441 ctcgccgcgc gcgcgcatgt tcttcagcga gatcggcgcg cacgccaacg acttcgggcg
     1501 cgtgttcgtc accggctacg ccctgctgct gttcacctgg tgggaaagca aggacgcgcg
     1561 cgccaggctg gcgctgttcg tcgcgctcgg catcctgtcg ctcggcatcc tgctcacctt
     1621 ctcgcgcaac gcgtacctcg gcttcttcct ggtgaacggc ctgttcctgc tgtggaagtt
     1681 caacatgaag aagctcgggc tcgcgctgct cggcaccgcg gtggcggcgg cgctcgcgcc
     1741 cggcgcggtc taccggcgca tcacctacgg cttcgactcc ggcagcgccg acaccgtgtc
     1801 ggccgggcgc atcgagggcc tgtggctgcc gctgctgccc gagaccctga agagcccgct
     1861 ctggggccag ggcctcgact cgatcatgtg gtccgacacc atggcgctcg gcgcgatgga
     1921 aatggcgggg catccgcaca acgcctacct gcaggcggtg ctcgacatgg gcgtgatcgg
     1981 cctggtgctg ctgctcgctt actactggca cgtgtggagg aacttccgcg cgctgggcag
     2041 caatccctac ctgacgccgc cgatgcgcgg gttcttccag ggcgcgtgcg ccgcgctgct
     2101 cgcgttcgcc gcggcctgca tgaccggcgg gagcctgcgg ccgcaggccg acaacgtgct
     2161 cctctggatc gccatcggca tcatgtacgg gctgctggtg aggaagccca agagctaaat
     2221 gcttcacgcc gagagggtgc gcgcgggcag cgcggagcgc tccggcccgc aggccttgtt
     2281 gtcccgaaag cgcgtctgct tcgtcgcgcc gcgcgcctgg ccggtgctgt cgggcgacgc
     2341 cgcgatcccg atcgtcggcg gcgccgaggt gcagcaaagc ctgctcgcgc gcctgctggc
     2401 cgcgcacggc caccgcgtgt cgatgatctg cctcgactac ggccagccgc agcgcgcccg
     2461 ggtcgacggc atcgaggtga tcaagacctt cggcatgacg cagggcctgc cgatgctgcg
     2521 cttcctgcat ccgcgcgtta cctcgatgtg gcgcgcgatg cgcgaggtcg acgccgaaat
     2581 ctactatttc cgctcggcct cgatgtggct cggcgtgctc gccgagttct gccgccgtca
     2641 cggccggcgc tcgatctacg ccggcgcctc caacaaggac ttcgtcgccg acgtcggcgg
     2701 ccagctgccc tacctgcgcg accgctggct ctaccgccgc ggtctcgccc aggccgaccg
     2761 catcgtcgcg cagaacgagg cgcagcgcgc cagcctgcgc gcgacgcacg ggcgcgacgc
     2821 ggtggtgatt ccgagctgct accaggcgcc cgcgcggcgc ggcgccgcgg cgaatcccgc
     2881 ggcgcgcgac cgcgtcctct gggtcgggac ggtgcacgag aacaagcgcc cggagctctt
     2941 cctcgagctc gccgcgcggc tgccgcaccg gcgcttcgtc ctgatcggcg ggccgcgccc
     3001 gggcggcgag gcgttctacg agcgcatccg cgcgcaggcg gccgcggcgc cgaacgtcga
     3061 ggccaccggc ttcctgcccc tcgccgaggt cgagccctgg ttcgaccgcg cccgcgtgct
     3121 cgtcaacacc tcgacctacg agggcatgcc gaacaccttc ctgcaggcct gggcgcgcgg
     3181 cgtgccgacg gtggcgaccg tggacgtcgg cgcgccggtg catcaggtgg tgccgaatcc
     3241 cgaggatctt cccgggcggg ttgaggcggc tttcgacaac cccgcgctcg gcgcgcgctg
     3301 ccgcgagtac ttcgagcgca accactcgcc cgccggggtg ctcgcgcgct acgaggagct
     3361 cttcgcctcg ctatgaacac cgcggccaag gcgatctcgt ggaacggtct caggcgccgc
     3421 gctttcgggc tcggcgcggt gaagacgttc gaccacgcca cgcacttcct gctgccggtg
     3481 gtgctcacgc gctgcctgga cgcggccacc ttcggcgagt accggctgct gtggctgatc
     3541 gtcggcacgc tgatgaccgt ggccacgctc aacatggccg ggggactgtt cttcttcgtg
     3601 ccgcgctcgg atgcgcgcgg caagcggctc tacatccacc agaccatgct gttcctcgcg
     3661 gcttcgggcc tggtctgcgg cgcgctcagc gggccgtgga acccgctgct gccggcggcg
     3721 gtcgcgccgg tcgcgcagta cggctggctg gtgccggcgt tcgtcgcgct gtgggtggcc
     3781 gcgatcctgc tcgatttcct tcccaccatc gacgagcgca tccgctggca ggccttcgcg
     3841 acgggcacca cctcggtgct gcgcgtggcg ctggtgggcg ccgccgcctg gttcaccggc
     3901 gagctgcgcg cggtgctgtg gatgctgctc gcgttcgtgc tggtgaagct cgcgctgctc
     3961 ctctactacg tgcaccgcca ccacgggctg gggcggccgt ggttcgagcg cgccgcgttc
     4021 ggcgcgcagt tcagccattg cgcgccgttc ggcgtgtcga acgcgcttta ttcgctccgg
     4081 gcgcagaccg accagtgggt cgcggcgagc ctgttcgcgc tcacgagctt cgccgcgttc
     4141 tcggtcgccg cgatcgtcgg ccacgtggtg cagatcctgc gccactcggt gatggaagcc
     4201 ttcatgccga cgatgagccg catgcaggcc gcgggcgacg tgcgcggcat gatggcgatg
     4261 aacagccgcg ccaacgcgat ggtcggcacg atgctctatc cgctgctcgc gctcgccttc
     4321 gccttcgccg aggacatcat cacgctggtc tacaccgccg cctacctcga ggcggcgccg
     4381 gtgatgcggg tctacgtcgt cggcatgctc gcgatggtgg tcgaggtcgg cagcatcgtg
     4441 ctgctgctgc gccaaggtcc gttcgcgctg cgcatcacgg cgctggtgct gccggtgtcg
     4501 gtcgcggtga gctggctcgc cgcgcacgag ctcgggctgg cgggcgcggc cgccggcagc
     4561 gtcgtcgcga tctacctcga ccgcgcgctg ctgctgcgcc gcgtcgcgcg ccataccggc
     4621 atcgcgctgc gcgagctgca ggcgtggcgc gccctcggcc gcacgctcgc gctcgcggcc
     4681 gcctgcggcc tgctcgcgtg gttcctggtg cagcagttcc tcggcggcgc ggcgccgctg
     4741 gtgcgcctcg ccgcgggcgc gacgatcctt gccctggcct acgtaccgct gtggagagcg
     4801 acccggtgaa gctgctcttc gtgacgggct cgctggtgca cggcggcgcg gagcgccaca
     4861 ccatcgggct gctcaaccgc ctggcgcagc gcgggcacga gtgccacgcc gcctatgtgc
     4921 gcgacgacgc gagccagctc gaccggctgc agggcgccgc gacggtcgag tgcctgcgcg
     4981 cgggcggcta cctcgatgcc gcggcgctgc ggcgcctcgg cgcgctcatc cgccggctcg
     5041 cgccctcggc ggtggtcgcc gccaatccct acgcgatgat gcatgccgcg ctcgcgctgc
     5101 ggcaatcggg cgtgcgcgcg ccgctcgcgg tgaccttcca taccacgctg ctgcccgggc
     5161 tcaaggagca gctgcagatg ctctactacc ggccgctctt ctggagcgcc gacgcgctgg
     5221 tgttcgtgtg cgaagcgcaa cggcgccact ggcgcaggcg gctggtgttc gggcgcgcga
     5281 gcgaggtgat ccacaacggc atcgacccgg agtactggcg cccgccggcg cccggggtcg
     5341 gcgcgcgcgt gcggcgggtg ctcggcttcg aggacggcga ctacgtgatc ggcatgtgcg
     5401 cggtgttccg gccggagaag aaccacaagc agctggtcga ggcgctggcg cagctgcggg
     5461 cgcgcggcgt gccggcgcgc gcgctgctca tcggcgacgg gccgctgcgc ggggcgatcg
     5521 aggcgcgcgc ccgcgcgctc ggcgtggccg gcgacgtgat catcgccggt ttccagcagg
     5581 acgtgcggcc gctgctcgcg gcgagcgacg ccgtcgcgct gtgcagcacg tctgagactt
     5641 tttcgctcgc cgcgctcgag gcgatggcgc tcggccggcc ggtgatcctg cccgaggtcg
     5701 gcggcgcggc cgagatggtg cgccacgggc gcgacggctt cctcttcccg gtgggcgaca
     5761 ccgccgcgct ggtgagccgg ctggcggcgc tggccgacgc gggcctgcgc cggcgcttcg
     5821 gcgccggcgc gcgcgagagc gtcgaagcgc gcttcacgga gcgcgccatg gtggaccgct
     5881 acgagcggct gctgcagcga atcgttttga aaaggagtca acgtgagaac ctacgaagaa
     5941 gtgcaacagc tcattaggag ccggcgccgc acctggctgg tgaccggggc ggccggattc
     6001 atcggctcca acctggtgga aaagctcctg tccctggagc aggacgtcgt cggtttggac
     6061 aacttctcca ccggcaagcg cgagaacctg cagcccggga tgcgcctgct cgagggcgac
     6121 atccgctcgc tcgacgactg ccgcgccgcg gtgaagggcg tcgacgtggt gctgcaccag
     6181 gccgccctcg gcagcgtgcc gcgctcgatc gagcgcccgc tcgacacgca cgacaacaac
     6241 gtgaacggct tcctcaacat gctggtcgcg acgcgcgacg ccggcatccc gcgcatggtg
     6301 ttcgcgagct cgagcgcggt gttcggcgac gacccgcagc ttcccaaggt ggaggagcgc
     6361 atcggccagg cggcctcgcc ctacgggctg tcgaagcaca tcaacgagct gtatgcccaa
     6421 gtcttcgccg cctgctacgg cttcgagtgg gtcggcctgc gctacttcaa cgtgttcggg
     6481 ccgcggcagg acgccgacgg cgcctacgcc tcggtgatcc cggcctggat cggcgcgctg
     6541 ctgcgcggcg agagcgccta catcaacggc gacggctcgt gcgcgcgcga cttctgccac
     6601 gtcgacaacg tggtgcagat gaacttgcgc gccgcgacca ccgaggaccc ggcggcgctc
     6661 aaccagccct acaacgtggc gcacggcgag atcacctcgc tcaccgagct gttcgagacc
     6721 atccgcacgc tgctcggcaa gcgcctggcg cacctgaagg gcgtgcgcgc ggtataccgc
     6781 gagccgcggc gcggggacat ccagctctcg cgcgccgaca tcgcaaaggc gcaacgcctg
     6841 ctcggctacg agccgaccat gcgcctcatg gacggcctgg agaagaccat ggactggtac
     6901 gtcgcgaacc tcgcgccgcg cgaggaaagg aaggtcgccc atgctgcttg aagcgcaggc
     6961 ccccctgcgg gaggagcaga agctcgccac ggtggcggtg ctcggcctgg ggtacgtcgg
     7021 cctgccggtg gcggtggcgt tcggccggca gcgcccgacc atcggctacg acatctcgcg
     7081 caagcgcgtg gagaacctga agcaccgcgt cgacgtcacc ggcgagctga gcgccgccga
     7141 gctcgccgag gcgcgaaact ggcatccgac cagcgatccg gccgagctcg gcaaggccga
     7201 tttcatcatc gtcgcggtgc cgacgccgat caacgacgcg cgccagcccg acctgcgccc
     7261 gctgcagtcg gcgagcgaga ccgtcggccg ccacatgaag gccggcgcca ccgtgatcta
     7321 cgagtcgacc gtgtaccccg gctgcaccga ggaagtgtgc gtgccgatcc tcgagcgcag
     7381 ctccggcatg cgctggcgcc aggacttcca cgtcggctat tcgccggaac ggatcaaccc
     7441 cggcgacaag gaacacgttt tcgaaaagat caggaagatc gtctcgggcg acgacgcggc
     7501 gacgctcgag gcggtggcgc agctctacgg ctcggtggtg aaggcgggcg tgtaccgcgc
     7561 ctcgtcgatc cgcgtcgcgg aggccgccaa ggtgatcgag aacacgcagc gcgacctgaa
     7621 catcgcgttc gtcaacgagc tcgcgatcat cttcgagaag ctcggcatcg acacgctgga
     7681 agtgctgaag gcggcgggca cgaagtggaa cttcctgccg ttccgcccgg gcctggtcgg
     7741 cggccattgc atcggcgtcg acccgtacta cctcacctac cgcgccgagc tcgccggcta
     7801 ccaccccgag gtgatcctcg ccgggcggcg catcaacgac gccatgggca gccacatcgc
     7861 gcgcaagacc gtgcagcaga tgatccacgc cggccgcaac atcaagggcg cgcgcgtcaa
     7921 cgtgctcggc ctcacgttca aggagaacgt gcccgacatc cgcaactcga aggtgatcga
     7981 catcatccgc gagctgcacg agttcggcgt cgagaccttc atccacgatc cgatggccgg
     8041 cgccgacgag gcgctgcacg agtacggcgt gcgcctggcc gagtgggaca gcctgcccgc
     8101 ggccgatgcg ctgatcctcg cggtgccgca ccagcagttc ctgcggcgca ctgcggccga
     8161 gctgatggag aagatcgtgc gcggcggctg cgtgatcgat gtcaaggcgg tgctcgatgc
     8221 gcagcccttc cagcgcgagg ggctgcgcgt atggcgcttg tagcccgtgc gagcgccgcc
     8281 cgaccggcaa ggcttcgggt aggcgtcttc gccgactcgc ggctgcagcc gcgctggatc
     8341 gtgcaggcgc tcgaccgggt cgcgcgctgc ggctgggccg aggtcgtcgc ggtggtcgcg
     8401 ccgcaggggg tttttccaaa aaacccctgg tgggcgcgcc tcgaccgctg ggccttcggc
     8461 tcggatcttt gcgagccggt gcgcctggcc gccgcggaca gcgcggaccg cgtcgcgctc
     8521 gatctggacg tcgccttcgc gctcggcgag ctcgacgacc gcgcgctcga cggcatcgcg
     8581 cgcctgggcg tatggcgcat ccgcgccgac ggcgcgcgcg aggtggcgaa ccaggagccg
     8641 gtgagcggcg ccgcgatcac ggtgcgcctc gccgccggcg aagcgccgcg gctcgcctgg
     8701 gagtcgtggg gtcgcaccta tcccctgtcg gtggcgcgca accggcgcga gctgttcgag
     8761 cgcgccgtgg agctgccggc gcgcgccttg cgcgacgcgc agcgctacgg cggcgcgtgg
     8821 ctcgagcagc ggcctttcgc gaaggcggat cttccgcagg cggcgccgtc gctcgcggca
     8881 gtatcgaaaa gaatcctgca gcgaggcgtg gaaaaggcgc tgtaccagga acaatggtcg
     8941 ctcgcctggc gcttcggcgc gctggcgccg gacctcgagg gtttcacgcg cctcgtgccg
     9001 ccgcccgggc ggaactgggc acatcccttc gcgctcgcgc gcggcgggcg ctactacgtg
     9061 ttcttcgagg acggcggccg catcgcgatg cttcaggtgg aagagggggg cgcgtggtcc
     9121 gcggcgcgca ccgtgctcga gcgcgactat ccgctgtcgc acccgttcct gctcgagcac
     9181 gaggacgcgc tgtacatggt gccgcagacg gccgacaacg gcaccgtcga gctctatcgc
     9241 tgcgtcgact ttcctttcct ctggcgcctg gagcgcgtgc tgctcgaggg ggtgcgtctg
     9301 gccaacccga cggtgcaccg cggcgtcgat cgctggtgga tcttcgccgg cgtgggcgag
     9361 catgaattgc atctttttca cgccccgcgc ctgtccggcg gcgagtggca gccgcacgcg
     9421 cgcaacccgg tgaagtcgga cgtgcgctcg agccggccgg cgggcgcgcc ctactggcga
     9481 aacggcgcgc tctaccgccc ggcgcagatc aacgcgccgc gccacggcgc cggcgtcgcc
     9541 ctgaaccgcg tgctgcggct gagtgccggg gagtacgccg agcgccaggt ggagcggatc
     9601 ctgcccgcgg gcgacctgct cggcatgcaa acggtgaacc gcgcgggcgc gctcaccgtg
     9661 atcgattttc ttacgcgccg caaccgcgtt tgagccgttc cgcctagccc gcggtaaccg
     9721 cggcatcacg cccggccggg atacttggaa gccatgagct cctattccag actggtcatg
     9781 ctgggcgccg cgcccgagac ccgcggcagc attgcggcgg tggtgcaagc ctaccgcgcg
     9841 cacggcctct tcaagcgctg gcagatcgag tacctgccga cgcacagcga ccggggcttc
     9901 ggccacaacg ccggcctcgc cgtttcggcg gtgcagcgct tcagctcgct gctcgcgcgg
     9961 cacggacgca tgccggtgca cctgcacatg gccgcgcgcg gcagcgcgct gcgcgacgcg
    10021 gctttcatcg gcgccgcgct cgccgcccgc tgcccgatcc tggtgcagct ccacggcgcc
    10081 ggcttcgagc gctcgcacgg cggccccctg atcgcgcgca tgctcgagcg cgcgagcttc
    10141 gtcgtcattc cctgcgagtc gatgcgcgcc tgggtgcgca gcgtcgcgcg ccacgcgcac
    10201 acggtgtgcc tgccgacccc ggtctggtgc gagctgcaac cctcctcgcg cccgaacctg
    10261 gtgctgtttc tcggccgcat ggaagcttcg aagggcatct acgacctgct ccaggcg
//
LOCUS       DASRIE010000130         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3801415, whole genome shotgun sequence.
ACCESSION   DASRIE010000130 DASRIE010000000
VERSION     DASRIE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801415"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..586
                     /locus_tag="VFB53_05650"
     CDS             110..586
                     /locus_tag="VFB53_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZN86255.1"
                     /translation="MATRAEKDACDLLDADHRAVKKMFKEYEELTNSRGRTAAQKKLD
                     LARQICHELTVHAQVEEELFYPALRAVLKETDLLAEAEVEHKTAKDLIAQIEAMGEAD
                     EMFDAKVTVLGEYIDHHVKEERNEIFPKARSARKLDLVAMRDELMARKEELMGAMA"
     gene            761..3277
                     /locus_tag="VFB53_05655"
                     /pseudo
     CDS             761..3277
                     /locus_tag="VFB53_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            3339..>3439
                     /locus_tag="VFB53_05660"
     CDS             3339..>3439
                     /locus_tag="VFB53_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN86256.1"
                     /translation="MNLRPLGRSGLEVSTLCFGGNVFGWTTDAAQSAA"
BASE COUNT          426 a         1244 c         1259 g          510 t
ORIGIN      
        1 gcggcgcgta cgtgcgtcgg cctgctgcgg ccgcagctgc cgccgttttc ctacaggggt
       61 ccacccggcc cctgcccatg atgcgggacc gacccaaagg agaacgacca tggccacccg
      121 tgccgaaaaa gacgcttgcg acctgctgga cgccgaccat cgtgccgtca agaagatgtt
      181 caaggagtac gaagaactca ccaattctcg tggccgcacg gccgcgcaga agaagctgga
      241 cctggcccgc cagatctgcc acgagctcac cgtgcacgcg caggtcgagg aagaactctt
      301 ctatccggcg ctgcgcgccg tgctgaagga aaccgacctg ctggccgagg ccgaggtcga
      361 acacaagacc gccaaggacc tgatcgcgca gatcgaagcc atgggcgagg ccgacgagat
      421 gttcgacgcc aaggtgacgg tgctgggcga atacatcgac caccacgtga aggaagagcg
      481 caacgagatc tttcccaagg cccgctccgc gcgcaagctg gacctggtgg ccatgcgcga
      541 cgagctgatg gcgcgcaagg aagaactcat gggcgcaatg gcctgaggct tgcctgctgg
      601 ggttcggctt cgccgctcac cccagcctac cgggcctggc cttcgccggg gcttcggttg
      661 tccgcgatga tgacgccgcg gtgacgttcg ccgtaggctg gcggtgagcg cggagccgaa
      721 ccccagcgat ccccgaccgc gccaacggca caatgccgtc gtgctcgccc tcctcacgac
      781 ctattcctgg caggaagtcc gctaccaccc gtggcgcagt gccaccgccg tcctggcggt
      841 catgctcggc gtcgcgctcg ccttttccgt gcacctgatc aacgcctcgg cgctggacga
      901 attctccagt gccgcgcgct ccgccggcgg ccagccggac ctggaactgc gggcggcgca
      961 gggggcgatg gacgaggcgc tctacgcgcg catcgcggcc gacccgcggg tcgccctcgc
     1021 cagcccggtg ctcgaactgc agaccctggt cctcacgcgc gagggccggc gcgcgaacgt
     1081 gcgcgtgctc ggcgtcgacg cgctggtggt cgccgcagtc gcaccgcagc tgatgcccat
     1141 gccggcggaa ggcggcgatc gcctggcggt gctggcaccg gacacggcct tcctgaacgc
     1201 cgtcgcgcag cagttgctgc ccgccggcac cgtgcgcctc caggccggcc tggtgctgcg
     1261 cgaactgcgc gtggccggca cggtgggttc cggcggcggg ccgctgctgg tgatggacat
     1321 cgcggcggcg caggacttct tcggccgcgg cggacagctc acgcgcatcg acgtgcgcct
     1381 gaaaccgggg gtcgcccgcg ccgactggct gcgcgagctg gacctgccac cgcaggtgca
     1441 ggccgccgag cccggcgatg cgggccagcg cgtgagccag ctctcgcgcg cctatcgcgt
     1501 caacctcacg gtgctggccc tggtggcgct gttcaccggt gccttcctgg tgttctcggt
     1561 gctggcgctg agcgtggccc gccgcgcgca gcagttcgcg ctgctggggg tgctgggcct
     1621 cacgggcagg cagcggctgc acctggtgct cgccgagtcc gccgtgctgg gcatcgcggg
     1681 cagcgcgctg ggcatcgcac tgggctcggc gctggcggcc gcggcgctct ccctgctggg
     1741 aggcgatctc ggcggcggct acttccccgg cgtgcagccg cggctgcagt ggaacggggt
     1801 agcggccgcc gggtatggcg ccctcggcgt ggtggcggcc atggccggcg gctggctgcc
     1861 cgcgcgccac gccgcgcggc tgccgatcgc gcagacgctc aagggcctgg gcaccgccct
     1921 gccccgcggc cgccgccacc tgctgggatg gctggcgctg gccgcggccc tggtgctcgc
     1981 attgctgccg ccggtggcgg gcatcccgct ggccgcctat ctctccgtgg ccttgctgct
     2041 ggtgggcggc atctcggtgc tgccgggcgc cgtcggtgcg ctgtacgacc gcatcgcgcc
     2101 gcgcgtggcc catcgcctgc tgccgctgct ggcggtggag cgcgcgcggc gcgtgcgcga
     2161 aagcgccgcc gtcgccgtca gcggcgtggt ggccgcgctc agcctggcgg tggcgctcac
     2221 ggtgatggtc gcgagcttcc ggcaatcggt cgccgactgg ctggacgtgc tgctgcccgc
     2281 cgacctgtac gtgcgcacca gcaacagcac cgcgtccggc gacaccgcct tcctcacccc
     2341 cgagctggtc cagggcctgg gctcgctgcc cggcgtggcg cgcgtggcca cccagcgcgt
     2401 gcgcagcctg ctgctcgatc ccgcgcagcc gccggtgacg ctgctcgcgc ggccggtgga
     2461 cgacgcgcag cgcaccctgc ccctggtcgg cgacacggcg tcggtgccga ccgacaccat
     2521 ccccgtgtac gtgagcgaag ccatggtgga cctgtacggc gcgcgccgcg gccagcggct
     2581 ggaggtgctg gagtccgcgc tgggcaccgg caggttcgcg gtcgccggcg tctagcgcga
     2641 ctacgtgcgc cagaccggcg cggtgatcat ggaccaggcc gactacctgc gcctcaccgg
     2701 cgacacgcgc gtcaacgacg cccagctctg gctggcgcca ggcgagtccg ccgacgaagt
     2761 gcagggccgc gtgcgcgcgc tggtggagcg cgaggcgggc gacccgggcc tggtggaatt
     2821 cgcctccgct gcgcaggtcc gcgcggtctc gctgcgcatc ttcgaccgca gcttcgcggt
     2881 cacctactgg ctgcaggccg tggcgatcgc catcgggctc ttcggcatcg cggcgagctt
     2941 ctccgcgcag gtgctcgcgc ggcgcaagga gttcggcctg ctcgcgcacc tgggcctcac
     3001 gcgcgggcag atcctcgccg tggtcgcctc cgagggtgcg gtgtggaccg cggcgggcgc
     3061 gctcgtgggc ctcgcgctcg gcatcgcggt gagcgtcgtg ctcgtgcacg tggtcaatcc
     3121 gcagagcttc cactggacga tggacctgct gctgccctgg gggcggctcg cgctgctgtg
     3181 cgcggcggtc gtcgtcgcgg gcacgctgac cgcgtggctc gcggcgcgca gcgcggcggc
     3241 gggctcgatg gcgctcgcgg tgaaggagga ctggtgatgc ccctgcggcc ggcgctgcac
     3301 ctcgtgcatg gcataacgaa cgtgcaagga ggacgcccat gaacctgcgc ccgctcggcc
     3361 gctccggcct cgaggtctcc accctctgct tcggcggcaa cgtgttcggc tggacgaccg
     3421 atgccgcgca atcggcagc
//
LOCUS       DASRIE010000131         9154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3802514, whole genome shotgun sequence.
ACCESSION   DASRIE010000131 DASRIE010000000
VERSION     DASRIE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802514"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFB53_05665"
     CDS             <1..427
                     /locus_tag="VFB53_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86257.1"
                     /translation="QPLAPALAVTRIEEDNSTVTFSPSGAWVERQREVAGFNNGRALT
                     YNLSGGTATFSFTGTGVSWIGLKCNLCGIANVSIDGGAPTMVNTAGPAGPGSFGLRSE
                     PVFTASGLAAGPHTLQIAVTGTTSSGDTHIAVDAFDVTP"
     gene            complement(434..1285)
                     /locus_tag="VFB53_05670"
     CDS             complement(434..1285)
                     /locus_tag="VFB53_05670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86258.1"
                     /translation="MPESDRLSGRMSMPGSFDSAFSTDENRAAYLANNRIAIGPPGDP
                     AEFRREYARFERLLPARAPLRVLDIGCGTGAWSVHWVARGCRVTGVDFDPDFLARAQM
                     RDGLGDPETFRTVAADATKLPSDIGKYDVVMLNSLLEHVPDWHGAVSGAARAVAPGGV
                     FILHTTNRYHPFQGEVNNFPFYPWLPGPIRDRVLAWIMKHRRDMVNYTDYPAVHWFTH
                     PQLARALREFGLEVYDRLDLMRPEQMTGLRKYARWMLAGDGRPARGKIFFYLLTRSVS
                     LYAKRPE"
     gene            1499..2056
                     /locus_tag="VFB53_05675"
     CDS             1499..2056
                     /locus_tag="VFB53_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86259.1"
                     /translation="MQLEQLRSEDTGRLAPARVAAPLTLAYIGRLARETGIGEVVNGL
                     RLACDGGVWPRLVIAGSGEDESCLRKLVERLQLSEQVHFAGPVFAEARLKILRHADIS
                     VLPRCGTGLLQTLHYSLLAGVPVIAPRTGTVSDAIVHKVHGLLVPSGDPAEYGRAMCQ
                     LAADRELLAHMREACRAWPGARASA"
     gene            complement(2046..2888)
                     /locus_tag="VFB53_05680"
     CDS             complement(2046..2888)
                     /locus_tag="VFB53_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HZN86260.1"
                     /translation="MSAFLELEARAMREDFDRRPFALRHRLCAHPLFALPRLLALARR
                     LPPECIETNAGDLPVDQDPRATPRNGLSAEETLRRIAQCGSWMVLKNVQRDPDYAALL
                     QGCLAELRAPDARLAQGFVFVSSPGAVTPFHMDPEHNFLLQVAGRKTIHVFDPADREV
                     LAEEDLERFHAGAHRNLPWREAWQPRARAFALGPGDGVHVPVTAPHWVRNGDAVSVSF
                     SVTFRSRASERRAQVYAMNARLRRRGRVPAPVGASALRDALKGFSLRLIRRAERLLAR
                     PATR"
     gene            complement(2885..4096)
                     /locus_tag="VFB53_05685"
     CDS             complement(2885..4096)
                     /locus_tag="VFB53_05685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN86261.1"
                     /translation="MTADLGIALREPRPARGPLDEAGTERQDQARLRVTVLRSAREIE
                     AQSADWEDLAAHAVEPNPFYEPWMLLPALEAAGADPDKAPELVLVHAEGRTGRSRLVG
                     LFPLERRPRYRGLPLGELRLWRHELCFLCTPLLRSGFEREALEAFLGWLARDSGASLL
                     RLHYVPGEGLFHRALLRHFERHPQLMHFTERYTRALFRPRRSAEGYLRAAVRGRRLKE
                     FRRLARRLADQGRLEYRQLGRSEHAADWIGAFLALEARGWKGRAGTALASTNANRAWF
                     ERIALEAGRRGRLLIHGLYLNGRALALSCKIRAGDGAYAFKIAYDEDYEQYSPGLLLE
                     LEQIVRMHGSPRIGWLDSCAVPEHFMANRLWLDRRALETVTVAAGGAGALLVMLLPLL
                     RWARRRLARAP"
     gene            complement(4107..5267)
                     /locus_tag="VFB53_05690"
     CDS             complement(4107..5267)
                     /locus_tag="VFB53_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86262.1"
                     /translation="MAAVRVLREDDVPAAVELFERIYPQHRWASRGDCEDYFHEILFA
                     NPWRDAALPSWAAFDGSRLCGLYAIVPRRMQLRARALRAAVGCQFMVDAGPRRNLVAL
                     QLARACLAGPQDLTFADGANDLTRRMWRGVGGSVSMLHSLHWTRPLRPVRYALTLLAR
                     RRAPALAAAAGALGALPDALAARLRPNRFLHAAPQAADGELRVEDMLAHLPEALRGAA
                     LQPAYDQAGLAWLLEQAGRKTRHGSLRARAAREGGRVIGWYLYYLSRGGAAEVLELAA
                     LEGCYERVLTRLLADAWCQGAAAAHGRLDARYAQELSSRHCWFRWDSTWTLLHTRDPE
                     IAQAIHTGEARLGRLEGEWWMRFLDEDRAAPAPAPAQAPLRAAVAAPRAAAR"
     gene            complement(5269..5532)
                     /locus_tag="VFB53_05695"
     CDS             complement(5269..5532)
                     /locus_tag="VFB53_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZN86263.1"
                     /translation="MTTVSAAPDPAIVERLGALFAQSFHIEVPSPDTDLLESGILDSF
                     QFVELLAQLEQHFGLRLRIDDIELDDLRTLARIARMVAAPPAS"
     gene            complement(5529..7124)
                     /locus_tag="VFB53_05700"
     CDS             complement(5529..7124)
                     /locus_tag="VFB53_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZN86264.1"
                     /translation="MSSRLLHEGFSARAQARPEATALAFKDETLSYGVLESASNRLAQ
                     LLVAAGCRPGDRVALLMPKSPAAIIAMLGALKAGAIYVPMDPDSPAVRLGRVLELADC
                     RCVLAAGPVGSRLQEALALANCAQRPLVGWMDELPAFADFTLRDLAAQPSGAPALARA
                     AGDAALILFTSGSTGQPKGVTLTHANVAAFLDWAIAYFGIREDDRVSQHAPLRFDIST
                     FDIYGALWSGAEVHLVPPELNLLPHKLAQFIRESRLTQWFSVPAVLNLLAKFEVVAQG
                     DFPDLRRVLFAGEVLPTPTLAHWMRRLPHARFTNLYGPTETTISSSYYTLPGCPADER
                     APIPIGRACAGEELLVLDDKLQPVAAGETGELCIAGAGLSPGYWRDARRTGEAFVPGP
                     GGERIYRTGDLARRDAQGLVYFQGRADTQIKSRGYRIELGEIEAALHSVRWLRESAVV
                     AVRSEGFEGWLICCAYACAQQDGALSTESLRRSLAALLPGYMLPARWLRCEALPKNES
                     GKIDRVALRAAFQGAEARVGQAA"
     gene            complement(7121..8140)
                     /locus_tag="VFB53_05705"
     CDS             complement(7121..8140)
                     /locus_tag="VFB53_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN86265.1"
                     /translation="MLTLQTFTTCPPGTQADPASWRRQLQEAARCSEEAGCEGMLVVT
                     DNAQPDPWLVSQLVIEATARLSPLVAVQPAYMHPYSVAKMVSTLAGLHGRRVHLNLVA
                     GGFKNDLAALNDQTPHDERYARLLEYAAILQGLLGSPQALSFEGRYYKVANLKLVPAM
                     PAELMPGFFVSGSSAAGMETARALRATAVEYPKPPAEQPAVEDDLPRGMRIGIIARAS
                     EEEAWAAALARYPEDRKGQLAHELAMKVSDSVWHRQLSALARETAPESKGPARPSYWL
                     VPFQNYKAVCPFLVGSYGRVAEEVARYAAAGYRSFILDAPAEREELRHVGAVLERAAR
                     HRLAA"
     gene            8332..8832
                     /locus_tag="VFB53_05710"
     CDS             8332..8832
                     /locus_tag="VFB53_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86266.1"
                     /translation="MSSTSEEQRKPAYPRSRAMAYRITVEADFVRAELAHRETVEETR
                     DFLQALLRYSESYTCLLIQVRTSKPVFHVERHGLVEYLQQIARTPAHRIALLAEGADL
                     LASHEYLELLARQRGVNVRSFRDEAEALHWLRERRRQPDRRQNAERRAGPERRQGPRR
                     AAQGGG"
BASE COUNT         1344 a         3253 c         3366 g         1191 t
ORIGIN      
        1 tcagcctctc gctccggctt tggcagtcac gcgcatcgag gaagacaact ccaccgtaac
       61 cttcagcccc agtggtgcct gggtggaacg gcaaagggaa gtggctggct tcaacaacgg
      121 ccgggctctg acctacaacc tgagcggagg taccgcgacg ttcagtttca ccggcacggg
      181 tgtgagctgg atcgggctca aatgcaattt gtgcggcatt gcgaacgtgt cgatcgatgg
      241 tggagcgccg accatggtga acacggccgg tcccgcaggc ccgggcagct tcgggctacg
      301 atcggaaccc gtctttacgg catctgggct cgcggctggt cctcacacat tgcagatcgc
      361 cgtcaccggg accaccagtt cgggcgacac gcacatcgcc gttgacgcgt tcgatgtgac
      421 gccctgatct gggctattca ggccgctttg cgtacagcga aaccgaacga gtaaggaggt
      481 aaaagaagat cttgccgcgc gctggccggc cgtcacccgc tagcatccag cgcgcgtact
      541 ttcgcaggcc ggtcatctgc tccggccgca tcaggtcgag acggtcgtac acctcgagcc
      601 cgaactcgcg caacgcgcgc gcgagttgcg ggtgcgtgaa ccagtgcacc gcggggtagt
      661 cggtatagtt caccatgtcg cgccggtgct tcatgatcca ggccaggacg cggtcgcgga
      721 tcgggcccgg cagccaggga tagaacggga agttgttcac ttcgccttgg aacgggtgat
      781 agcggttcgt cgtgtgcaga atgaacaccc cccccggcgc gaccgcacgc gccgcaccgc
      841 tgacggcgcc atgccaatcg ggcacgtgct cgaggagcga attgagcatc accacgtcgt
      901 acttcccgat atcggatgga agcttcgtcg catctgccgc gacggtgcgg aaggtctccg
      961 ggtcgccgag gccatcgcgc atctgcgcgc gcgccaggaa gtcgggatcg aagtcgacgc
     1021 cggtcacccg gcagccgcgc gcgacccagt gcacgctcca cgcgccggtg ccgcagccga
     1081 tgtcgagtac ccgcagcggc gcgcgcgccg gcagcagccg ctcgaagcgc gcgtattcgc
     1141 ggcggaactc cgcagggtcg cccggcgggc cgatggcgat gcggttgttc gcgaggtacg
     1201 cggcgcggtt ctcatcggtg gaaaacgcgg aatcgaagga acctggcatt gacatcctcc
     1261 ctgacagtcg gtcgctctca ggcacgcgac ggcaggccca agacgaagct cttcacgcca
     1321 ccgagcatgg cagcgcccca tgccgggcga gggcggtccg actagtcctt tcggactacg
     1381 ggggtcgcca accttcgatt tgccgtcgtg cggaccgcga caggcgatac ccgggcggcg
     1441 cgacatccat cggcaccccc cctgcctaag atgtgctgca cgtcacgcag ggggaacatt
     1501 gcagctggaa caattgcgca gcgaggacac cggccggctg gcgcccgccc gcgttgcggc
     1561 gccgctgacg ctcgcctaca tcggccgcct cgcgcgcgag accggcatcg gcgaggtggt
     1621 gaacggactg cgcctcgcct gcgacggcgg cgtgtggccg cggctggtga tcgccggcag
     1681 cggcgaggac gagtcctgcc tgaggaagct ggtggagcgc ctgcagctct ccgagcaggt
     1741 gcacttcgcc ggaccggtgt tcgccgaggc gcgcctgaaa atcctgcgcc acgccgacat
     1801 ctcggtgctg ccgcgctgcg gcaccggtct cctgcagacc ctgcactaca gcctgctcgc
     1861 cggggtgccg gtgatcgcgc cgcgcaccgg cacggtgagc gacgcgatcg tgcacaaggt
     1921 gcacggcctg ctggtgccct ccggggaccc ggcggaatac gggcgcgcca tgtgccagct
     1981 cgccgccgac cgggagctgc tcgcgcacat gcgcgaagcc tgccgcgcct ggccgggcgc
     2041 gcgcgctagc gcgtagcagg ccgcgcgagc aggcgctcgg cgcgccggat caaccgcagc
     2101 gagaacccct tcagcgcgtc gcgcaaggcc gaggcgccaa ccggcgcggg cacgcggccg
     2161 cggcggcgca ggcgcgcgtt catggcgtag acctgcgcgc gccgctcgga ggcgcggctg
     2221 cggaaggtga cgctgaagga caccgacacc gcgtcgccgt tgcgcaccca gtgcggcgcg
     2281 gtcaccggca catgcacgcc gtcgccaggc cccagcgcga aagcgcgcgc gcgcggctgc
     2341 caggcctcgc gccaggggag gttgcggtgc gcgcccgcat ggaagcgctc caggtcctcc
     2401 tccgcgagca cctcgcggtc cgccgggtcg aacacgtgga tggtcttgcg gccggcgacc
     2461 tgcagcagga agttgtgctc ggggtccatg tggaacggcg tcaccgcgcc gggcgaggag
     2521 acgaacacga agccctgcgc gaggcgcgcg tccggcgcgc gcagctcggc gaggcagccc
     2581 tgcagcagcg ccgcgtagtc cgggtcgcgc tggacgttct tcagcaccat ccaggagccg
     2641 cactgcgcga tgcgccgcag cgtctcctcg gcgctgaggc cgttgcgcgg cgtggcgcgc
     2701 gggtcctggt ccaccggcag gtcgccggcg ttggtctcga tgcactccgg cggcaggcgg
     2761 cgcgccagcg cgagcaggcg cggcagcgcg aacagcggat gcgcgcacag gcggtggcgc
     2821 agcgcgaagg gccgccggtc gaagtcctcg cgcatcgcgc gcgcctcgag ctcgaggaac
     2881 gcgctcacgg cgcgcgggcg aggcgccggc gcgcccagcg caacaggggc agcagcatca
     2941 ccagcagggc gcccgcgccc ccggccgcca cggtgacggt ctccagcgcg cggcggtcga
     3001 gccacagccg gttggccatg aaatgctccg gcacggcgca ggagtccagc cagccgatgc
     3061 gcggcgagcc gtgcatgcgc acgatctgct ccagctcgag cagcaggccc ggggagtact
     3121 gctcgtagtc ctcgtcgtac gcgatcttga aggcgtaggc gccgtcgccc gcgcggatct
     3181 tgcagctcag cgcgagggcg cgcccgttca ggtacaggcc gtgaatcagc agccgcccgc
     3241 ggcggcccgc ctcgagcgcg atgcgctcga accaggcgcg gttcgcgttc gtcgaggcga
     3301 gcgcggtgcc cgcgcgcccc ttccagccgc gcgcctcgag cgcgaggaag gcgccgatcc
     3361 agtcagccgc gtgctcgctg cggccgagct ggcggtattc gaggcgcccc tggtcggcga
     3421 gccggcgcgc caggcgccgg aactccttca gccgccggcc ccgcaccgcg gcgcgcaggt
     3481 agccctcggc gctgcgccgc ggccggaaca gcgcgcgcgt gtagcgctcg gtgaaatgca
     3541 tcagctgcgg gtggcgctcg aagtgccgca gcagcgcgcg gtggaacagc ccttcgccgg
     3601 ggacgtagtg cagcctgagg agcgaggcgc cggagtcgcg cgccagccag ccgaggaaag
     3661 cctccaaggc ctcgcgctcg aagccgctgc gcaacagggg cgtgcacagg aagcacagct
     3721 catgccgcca caggcgcagc tcgccgagcg gcaggccgcg gtagcgcggc cgccgctcga
     3781 gcgggaacag gccgaccagg cggctcctgc ccgtgcggcc ttcggcgtgc accagcacca
     3841 gttccggcgc cttgtcgggg tccgcgcccg cggcctccag ggccggcagc agcatccagg
     3901 gctcgtagaa cggattcggc tctacggcgt gcgccgccag gtcctcccag tcggccgatt
     3961 gcgcttcaat ttcccgggcg ctgcgcagca ccgtcacgcg cagccgcgcc tggtcctggc
     4021 gctcggtccc ggcctcgtcg agcgggccgc gcgcaggacg cggctcccgt agtgcgatcc
     4081 ccaggtccgc ggtcacggcg cacggcctag cgcgcggcgg cgcgcggcgc cgcgactgcg
     4141 gcgcgcagcg gcgcctgggc cggcgcgggc gcgggcgccg cccggtcttc gtcgagaaag
     4201 cgcatccacc attcgccttc cagccggccg aggcgcgcct cgccggtgtg gatggcctgc
     4261 gcgatttccg ggtcgcgcgt gtgcagcagc gtccaggtgc tgtcccagcg gaaccagcaa
     4321 tgccggctcg acagttcctg cgcatagcgc gcgtcgagcc gcccgtgggc cgcggccgcg
     4381 ccctggcacc aggcatccgc tagcaggcgc gtcagcacgc gctcgtagca gccctcgagc
     4441 gcggcgagct ccagcacctc ggccgcgccg ccgcgcgaga ggtagtacag gtaccagccg
     4501 atcacgcggc cgccttcgcg cgccgcacgc gcgcgcaggc tgccgtgccg cgtcttgcgg
     4561 ccggcctgct cgagcagcca ggcgagcccc gcctggtcgt acgccggctg cagcgccgcg
     4621 ccgcgcaatg cctcgggcag gtgggcgagc atgtcctcca cccgcagctc gccgtcggcg
     4681 gcctgcggcg cggcgtgcag gaagcggttg gggcgcagcc gcgcggcaag cgcatcgggc
     4741 agcgcgccga gcgcgcccgc cgcggccgcg agcgcaggcg cccggcgccg cgcgaggagc
     4801 gtaagcgcgt agcgcaccgg gcgcagcggg cgcgtccagt gcaggctgtg cagcatcgac
     4861 accgagccgc cgacgccgcg ccacatgcgc cgcgtcaggt cgttcgcgcc gtcggcgaag
     4921 gtcaggtcct gcgggccggc gaggcaggcg cgcgcgagct gcagggcgac gaggttgcgc
     4981 cgcggcccgg cgtcgaccat gaactggcag cccacggcgg cgcgcagcgc gcgggcgcgc
     5041 agctgcatgc gccgcggcac gatggcgtac aggccgcaca ggcgggagcc gtcgaacgcc
     5101 gcccaggagg gcagcgcggc gtcgcgccag ggattggcga acaggatctc gtggaagtag
     5161 tcctcgcagt cgccgcgcga ggcccagcgg tgctgcgggt agatgcgctc gaacagctcg
     5221 accgccgccg ggacgtcgtc ctcccggagg acgcggaccg cggccatcct aggaggcggg
     5281 cggcgccgcc accatgcggg cgatgcgcgc gagcgtgcgc aggtcgtcga gctcgatgtc
     5341 gtcgatgcgc aggcgcagcc cgaaatgctg ctcgagctgc gccagcagct cgacgaactg
     5401 gaacgaatcg aggatcccgc tctccagcag gtcggtgtcc ggcgaaggca cctcgatgtg
     5461 gaagctctgc gcgaacagcg cgccgagccg ctcgacgatc gccggatccg gggcggcgct
     5521 caccgtggtc acgctgcctg cccgacgcgc gcctcggcgc cctggaacgc ggcgcgcagc
     5581 gccacgcggt cgatcttgcc gctttcgttc ttcggcagcg cctcgcagcg cagccagcgc
     5641 gccggcagca tgtagccggg cagcagcgcg gcgaggctgc gccgcaggct ctcggtggag
     5701 agcgcgccgt cctgctgcgc gcaggcgtag gcgcagcaga tcagccagcc ctcgaagccc
     5761 tcggagcgca cggcgaccac cgcgctctcg cgcagccagc gcaccgagtg cagcgcggcc
     5821 tcgatctcgc ccagctcgat gcggtagccg cggctcttga tctgcgtgtc ggcgcgtccc
     5881 tggaagtaga ccaggccctg cgcgtcgcgg cgcgcgaggt ccccggtgcg atagatgcgc
     5941 tcgccgccgg gcccgggaac gaaggcctcg ccggtgcgcc gcgcgtcgcg ccagtagccg
     6001 ggggagaggc ccgcgccggc gatgcacagc tcgccggtct cgcccgcggc gaccggctgc
     6061 agcttgtcgt cgagcaccag cagctcctcg cccgcgcagg cgcgcccgat cgggatcggc
     6121 gcgcgctcgt ccgcggggca gccgggcagc gtgtagtagc tgctcgagat ggtggtctcg
     6181 gtgggaccgt acaggttggt gaagcgcgcg tgcggcaggc gccgcatcca gtgcgcgagc
     6241 gtcggcgtgg gcagcacttc gcccgcgaac agcacgcggc gcaggtcggg gaagtcgccc
     6301 tgcgcgacga cctcgaactt ggcgagcagg ttgagcaccg cgggcaccga gaaccactgg
     6361 gtgaggcgcg attcgcggat gaactgcgcc agcttgtgcg gcaggagatt gagttcgggc
     6421 ggcaccaggt gcacctcggc gcccgaccac agcgcgccgt agatgtcgaa ggtggagatg
     6481 tcgaagcgca gcggcgcgtg ctgcgacacg cggtcgtcct cgcggatgcc gaagtaggcg
     6541 atcgcccagt cgaggaaggc cgcgacgttg gcgtgggtga gcgtcacgcc cttgggctgg
     6601 ccggtcgagc ccgaggtgaa caggatcagc gccgcgtcgc ccgccgcgcg ggcgagcgcg
     6661 ggcgccccgg agggctgcgc cgccaggtcg cgcagcgtga aatcggcgaa cgcgggcagc
     6721 tcgtccatcc agccgaccag cggccgctgc gcgcaattgg cgagcgcgag cgcctcctgc
     6781 agccgcgagc ccaccgggcc ggcggcgagc acgcagcggc aatcggccag ctcgagcacg
     6841 cgccccaggc gcactgccgg gctgtccgga tccatcggca cgtagatcgc gccggccttc
     6901 agcgcgccga gcatcgcgat gatggcggcg ggcgacttgg gcatcagcag cgcgacgcgg
     6961 tctccggggc ggcagccggc ggcgacgagc agctgcgcca gccggttgct cgccgactcg
     7021 agcacgccgt aggacagggt ttcgtccttg aacgcgagcg cggtcgcttc cgggcgcgcc
     7081 tgcgcccgcg cgctgaagcc ctcgtgcagc aaccgcgagc tcatgcagcc agccggtgcc
     7141 tggcggcgcg ctcgagcacc gcgccgacgt ggcgcagctc ctcgcgctcg gccggcgcgt
     7201 cgaggatgaa gctgcggtag ccggcggcgg cgtagcgcgc cacttcctcg gccacgcggc
     7261 cgtagctgcc gacgaggaac gggcacaccg ccttgtagtt ctggaacggc accagccagt
     7321 aagaagggcg cgccgggccc ttcgactccg gggcggtctc gcgcgccagc gccgagagct
     7381 gccggtgcca gaccgaatcg gacaccttca tcgccagctc gtgcgccagc tggcccttgc
     7441 ggtcttccgg gtagcgcgcc agcgccgcgg cccaggcttc ctcctccgag gcgcgcgcga
     7501 tgatgccgat gcgcatgccg cgcggcaggt cgtcctcgac ggcaggctgc tcggccgggg
     7561 gcttggggta ttcgaccgcc gttgcgcgca gcgcccgcgc agtctccatg ccggcagccg
     7621 acgacccgga gacgaagaat cccggcatca gctccgcggg catcgccggc acgagcttca
     7681 ggttggcgac cttgtagtag cggccctcga aactcagcgc ctgaggcgag ccgagcagcc
     7741 cctgcaggat cgccgcgtac tccagcaggc gcgcgtagcg ctcgtcgtgc ggcgtctggt
     7801 cgttcagcgc cgcgaggtcg ttcttgaagc cgcccgccac caggttcagg tgcacgcggc
     7861 ggccgtgcag gccggcgagc gtgctcacca tcttggcgac gctgtagggg tgcatgtacg
     7921 cgggctgcac ggccaccagc gggctcaggc gcgccgtcgc ctcgatgacc agctgcgaaa
     7981 ccagccaggg gtcgggctgc gcgttgtcgg tgaccaccag catgccttcg cagccggcct
     8041 cttcgctgca gcgcgccgct tcctgcagct ggcggcgcca ggacgcgggg tcggcctgcg
     8101 tgcccggcgg gcaggtggtg aaggtctgta gggtaagcat ggcgatcaag ctacccgcgc
     8161 cgggtgcatt gccaatgcag acggaggctg gtccgatcgg cctacgcgct gaggcgcagg
     8221 ccgctgtcgc caccccccga cggggggtgc gcaatccata ggccttagga actattgcca
     8281 gtgtccaacg cgctacatgg ggatgcgact gcaggccggt aggctctcgt catgtcctca
     8341 acgagcgagg agcagcgcaa gccggcgtac ccgcgcagcc gcgccatggc ctaccgcatc
     8401 acggtggagg cggacttcgt ccgagccgaa ctggcgcatc gcgagacggt agaggagacg
     8461 cgcgacttcc tgcaggcgct gctgcgctac agcgagagct atacctgcct gctgatccag
     8521 gtgcgcacct ccaagccggt gttccacgtc gagcggcacg ggctggtgga gtacctccag
     8581 cagatagcgc gcacgccggc ccaccgcatc gcgctcctgg ccgagggcgc cgacctgctg
     8641 gcctcgcacg aatacctgga gctgctcgcg cgccagcgcg gggtcaacgt gcgcagcttc
     8701 cgcgacgagg ccgaggcgct gcactggctg cgcgagcggc ggcggcagcc ggaccggcgc
     8761 cagaacgcgg agcggcgcgc gggccccgag cggcgccagg gcccgcgccg cgcggcgcaa
     8821 ggcggcggct gaggcggcgg gggcaaagaa aaagggcgcg gcattgcgcc gcgccccggg
     8881 ggaaacccgt cgaacggtgc gccgggtgcc tgcacttagt ttggcccgat cccgggctgc
     8941 taacagcggg ccggccacct ggctccgttc catgtagttc gctgactaca ttggtcgcaa
     9001 ctatcctacg gcgacaattg cgggggaatg acacgcaata gtccggctgg cgcatggatc
     9061 cgagcgatat actggtccaa cgccgcgttg aggatcgctc aatgagaatt ctgatcgccg
     9121 acgatcatcc gatcgtgcgc cacggggtgc ggca
//
LOCUS       DASRIE010000132         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_665356, whole genome shotgun sequence.
ACCESSION   DASRIE010000132 DASRIE010000000
VERSION     DASRIE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665356"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VFB53_05715"
     CDS             complement(<1..1443)
                     /locus_tag="VFB53_05715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN86267.1"
                     /translation="MSRPAARDAQHEGTPMTDGRRTILVVDDDADARVIMRAALRKAG
                     FEVRLAESGHDALRQFRVEPSDVVMLDVDMPELSGHEVCALLRTEAGPLVPIVMVTGM
                     DDVASVETAYEHGATDFIAKPVNWALIGHRVRYLLRGHQALLDLRAAEARNAAILNAI
                     PDLLFELDIDGRCIDYRAPKASLLAGSRASLVGRTVARIMPPRAADVVMSSLHKALEQ
                     GSTTGEQLELQLAKGSTWFELSVSSKAVTAAAKPRFIVLARDITERKIAEARIARHAY
                     FDSLTGLPNRPSFLQRVDRQVKRARECGDKLAILFMDLDGFKNINDSMGHAAGDLVLQ
                     WAAERLREGLRQSDFLSRPAPLADADRRSEDINLARLGGDEFTALVLGVEHPEDAVAV
                     ANRLGHLMRRPFVLEGRELTLTTSIGIALFPDDGDDGATLLKHADTAMYHAKNSGRDN
                     SKLYSASLTSKLVDRMNLDAGLRAALERVDL"
     gene            complement(1440..>2652)
                     /locus_tag="VFB53_05720"
     CDS             complement(1440..>2652)
                     /locus_tag="VFB53_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86268.1"
                     /translation="DNQTNRDILQQQLQGWRMAVSCVEGGPQALAAMHEAARSGHPFD
                     LAVLDMHMPGMDGLDLAQHIQAQPELACTRLVMLSSTYAGIDERERVQGGILRYLSKP
                     IRRADLQRALTGVLASTTVEGPRPAKPQASMAGLRGHVLLVEDNPINQGVAKAMLAKL
                     GLQYQLAADGAQAVDRVCRSEFDLVLMDCQMPVMDGYQATAAIRALPGGRGAQLPIVA
                     LTANAMQGDEQVCMDAGMNGFLAKPYTLAALHNTLARWLSEAPAAMPAQAPPAAAPPS
                     VAKAPGAPQAPAINMAAIDILRELDEDAGPALARQLVSSFLASADDNLARVAAAAGEG
                     DAKVLSQAAHALKSSAANLGAESLSSCYRELEMCGREGRIDDARGLLELTGREHRRAQ
                     LRLQELLLELA"
BASE COUNT          341 a          916 c          981 g          414 t
ORIGIN      
        1 gaggtcgacg cgctcgagcg ccgcgcgcag gcccgcgtcg aggttcatgc ggtcgacgag
       61 cttgctcgtc agcgaggcgc tgtagagctt cgagttgtcc cggcccgaat tcttggcgtg
      121 gtacatcgcc gtgtcggcgt gcttgagcag ggtggcgccg tcgtcgccgt cgtcggggaa
      181 cagggcgatg ccgatgctcg tcgtgagcgt gagctcgcgg ccctcgagga cgaacggccg
      241 ccgcatcagg tggccgagcc ggttcgccac ggcgacggca tcctccggat gctcgacgcc
      301 cagcaccagg gcggtgaact cgtcgccgcc caggcgcgcg aggttgatgt cctcgctgcg
      361 ccggtcggcg tcggcgagcg gagccgggcg cgacaggaag tccgactgcc gcaggccttc
      421 gcgcaggcgc tcggcagccc actgcagcac gaggtcgccg gcggcatggc ccatcgagtc
      481 gttgatgttc ttgaagccgt cgaggtccat gaacaggatg gccagcttgt cgccgcactc
      541 cctggcgcgc ttgacctggc ggtcgacccg ctgcaggaac gacgggcggt tgggcaggcc
      601 ggtcaggctg tcgaagtagg cgtggcgggc gatgcgggcc tcggcgatct tgcgttcggt
      661 gatgtcgcgc gccagcacga tgaagcgcgg tttcgctgcc gccgtgacgg ccttgctcga
      721 caccgacagc tcgaaccagg tcgatccctt ggcgagctgc agctcgagct gctcgccggt
      781 cgtcgaaccc tgctcgagcg ccttgtgcag cgacgacatg acgacgtcgg ccgcgcgggg
      841 cggcatgatc ctggcgaccg tcctgccgac gaggcttgcg cgactcccgg ccagcaggct
      901 ggccttcggc gcccggtagt cgatgcagcg cccgtcgatg tcgagttcga acagcaggtc
      961 cgggatggcg ttgaggatcg ccgcgttgcg cgcttcggcg gcgcgcaggt cgagcagggc
     1021 ctggtggccg cgcagcaggt agcgcacccg gtggccgatc agcgcccagt tgacgggctt
     1081 ggcgatgaag tcggtggccc cgtgctcgta cgccgtctcg accgaagcca cgtcgtccat
     1141 gccggtgacc atcacgatgg gcaccagcgg gccggcctcg gtgcggagca gggcgcagac
     1201 ctcgtgcccg ctcagctcgg gcatgtcgac gtcgagcatg acgacgtcgc tgggctcgac
     1261 gcggaactga cgcagcgcgt cgtgcccgct ctcggccagg cggacctcga agccggcctt
     1321 gcggagcgcg gcgcgcatga tcacgcgcgc gtcggcatcg tcgtcgacga ccaggatggt
     1381 gcggcggcca tcggtcatcg gcgtgccttc gtgctgcgca tcgcgggcgg ccggtcggct
     1441 cacgcgagct ccagcagcag ttcctgcagg cgcagctgcg ctcgccggtg ctcgcgcccg
     1501 gtcagctcga gcaggccgcg cgcgtcgtcg atgcggcctt cgcggccgca catctcgagc
     1561 tcgcgatagc agctcgacag cgattcggcg ccgagattgg cggcgctgga cttcaaggca
     1621 tgcgcggcct ggctcaggac cttggcgtcg ccctcgccgg ccgccgccgc cacgcgcgcc
     1681 aggttgtcgt cggccgaggc caggaacgag ctcacgagtt gcctcgccag cgccgggccg
     1741 gcgtcttcgt cgagttcgcg caggatgtcg atggccgcca tgttgatggc cggcgcctgc
     1801 ggcgcccccg gcgccttggc gacggacggc ggcgccgcgg cagggggggc ctgcgccggc
     1861 atggccgccg gcgcttccga cagccagcgc gccagcgtgt tgtgcagcgc ggccagggtg
     1921 tagggcttgg ccaggaaacc gttcatgccc gcatccatgc agacctgctc gtcgccctgc
     1981 atcgcgttgg ccgtgagcgc gacgatgggc agctgcgcgc cgcggccgcc gggcagcgcc
     2041 cggatcgccg cggtggcctg atagccgtcc atcaccggca tctggcagtc catcagcacg
     2101 aggtcgaact cgctcctgca gacgcggtcg acggcctgcg cgccgtcggc ggcgagctgg
     2161 tactgcagcc cgagcttggc cagcatggcc ttggccacgc cctggttgat cgggttgtcc
     2221 tccaccagca gcacgtggcc gcgcaggccg gccatcgacg cctgcggctt cgccggccgc
     2281 ggcccttcga cggtcgtgga cgccagcacg ccggtgagcg cacgctgcag atcggcgcgg
     2341 cggatcggct tgctcaggta gcgcaggatg ccgccctgca cgcgctcgcg ctcgtcgatg
     2401 ccggcatagg tcgagctcag catcaccagg cgcgtgcacg ccagctcggg ctgtgcctgg
     2461 atgtgctgcg cgaggtcgag gccgtccatg ccgggcatgt gcatgtcgag cacggcgagg
     2521 tcgaacggat gcccgctgcg cgccgcttcg tgcatcgcgg caagcgcctg cggaccgcct
     2581 tcgacgcagc tcaccgccat gcgccagccc tggagctgct gctgcaggat gtcgcggttg
     2641 gtctggttgt cg
//
LOCUS       DASRIE010000133         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3804415, whole genome shotgun sequence.
ACCESSION   DASRIE010000133 DASRIE010000000
VERSION     DASRIE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804415"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFB53_05725"
     CDS             <1..1180
                     /locus_tag="VFB53_05725"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HZN86269.1"
                     /translation="PHVKIAASAAQVHVARLGTGDARLVIGCDLVVSASGDALDKMSA
                     GRTRAVVNGTTSPTAAFVRNPDWQLPGSNLAHDIAEACGGRNAEFVPAAQIATALMGD
                     SIATNMFMLGYAWQKGWVPLGREAIERSIELNGVAVEFNRQSFLWGRRAAVDLEQVRR
                     IAQPADVIPIEQHFSRNLQEIVERRAQFLTGYQNAAYATRYQVLVGRVRISEQDKVGG
                     TKLAEAVARNYAKLLAYKDEYEVARLYASGEFMRKIAGMFEGDYRLKFHLAPPLLAKP
                     DPATGEPRKMQFGPWMLRVFGLLARLRWLRGTPLDPFGGTQERRMERALIEEYEMAVD
                     ALLQGLARDNLALAVEIASIPESIRGYGHIKAKSVEAARNKQSALLARFQAAPVRAAA
                     "
     gene            complement(1204..2400)
                     /gene="phaZ"
                     /locus_tag="VFB53_05730"
     CDS             complement(1204..2400)
                     /gene="phaZ"
                     /locus_tag="VFB53_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZN86270.1"
                     /translation="MLYSLYEAQHAWLTPYRALAEMQRGWFANPFMPWAQAPLAKQLA
                     ASNDVFLRITQRYEKPAWRIEGADIEVALDKPFCNLLHFTPHSPRAAGLPKVLVVAPL
                     SGHHATLLRDTVRTLLPEHDVWVTDWVDARMVPLAAGPFHLADYVDYVREFIGLLSPE
                     VNVISVCQPTVPVLCAIALLAEEKTAPQPRTMVMMGGPIDARKSPTAVNNLAMTRPHS
                     WFERNVIQRVPSKYPGYMRRVYPGFLQHLGFVAMNPDRHLNAHWDYFNHLVEGDDDSA
                     DRHREFYDEYNAVIDLPAEYYLDTVKAVFQEFALPKGRLYIRDRLVRPQLIRDTALLT
                     IEGELDDISGNGQTESAHLLCLNIPRERRQHYLAPKVGHYGIFSGRRWRTLIYPRVRD
                     FIRSRG"
     gene            2470..4983
                     /locus_tag="VFB53_05735"
     CDS             2470..4983
                     /locus_tag="VFB53_05735"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZN86271.1"
                     /translation="MGLFLKLVFALLGLAGLALGGGYLVLRQSLPQEEGEIRLAGLGK
                     PVDVLRDRYGVPHITAASLGDAVYALGFVHAQDRLWQMEVNRRTAAGRLAEVFGEGAL
                     EADRFLRTLGVRRAAEANLQRLDAPTRELLERYADGVNAFLATKPVLPVEYWLTGAAA
                     EPWTPVDSLGWVKMMAWDLGGNWRNELLRMRLAKTMPNARIHEFLPPYPGEAPPEIAD
                     LRKLYGGMAKEGSRLAQLEGEGGASDLAFENDLRFFGDMNLVAKKTETMENQTQALPW
                     FDADAQTEGLGSNNWVVSGARSATGKPLLANDPHLGLSAPAVWYFAHLNAPGFDAIGA
                     TLPGVPAIVLGRNAHFAWGFTNTGPDVQDLYLERLDGAGNYLTPEGPKAFVLRDEVIK
                     VKGGADVRLQVRASRHGPVISDVSRPAQDAAPRGHVIAFQWTALREDDMTMQAAAKLA
                     RARDWPGFVTALRDYHSPQQNVVFADTEGNIGFVAAGRVPVRKPENDLKGLAPAPGWL
                     AQYDWAGFIPFAELPLAYNPADGVLMSANHKITPPGYGHYLTSEWQPPYRARRIAELL
                     GALRRHSIGSFARIQGDVVSLPAKDLLPRLGKVVPKSEAGRRALSRLAAWDGAMLADR
                     PEPLILAAWWREFARAVYADELGEAFRANWLTRAQFLTNVLGDKDRQGRWCDDVRTKG
                     AEGCDTLLAATLEAALADLGGRYGEDMDAWRWGTAHQARHEHRPFGRVRWLAPLFDIS
                     VPTPGDAYTVNVGRSRLEDGARPYANTHAASLRAIYDLSNPDNSLYIHSGGQSGNVLS
                     AHYRAFTQAWANGEYIPMVTDRGKIAAGGTRKLTLAPRQ"
BASE COUNT          799 a         1687 c         1785 g          738 t
ORIGIN      
        1 gccgcacgtg aagatcgcgg cgagcgccgc ccaagtgcac gtggcgcgcc tgggcacggg
       61 cgacgcgcgc ctggtgatcg gctgcgacct ggtggtgagc gcgagcggcg acgcgctcga
      121 caagatgagc gccgggcgca cgcgcgcggt ggtgaacggc accacctcgc ccaccgccgc
      181 attcgtcagg aacccggact ggcagttgcc cggctcgaac ctggcgcacg acatcgccga
      241 ggcctgcggc ggccgcaatg ccgagttcgt gcccgcggcg cagatcgcca ccgccctgat
      301 gggcgattcc atcgccacca acatgttcat gctcggctac gcctggcaga aaggctgggt
      361 gccgctgggg cgcgaggcga tcgagcgctc gatcgagctg aacggcgtgg cagtcgagtt
      421 caaccggcag agcttcctct ggggccggcg cgcggcggtg gatctggagc aggtgcgccg
      481 catcgcgcag ccggcggacg tgatccccat cgagcagcat ttctcgcgca acctgcagga
      541 gatcgtggag cggcgcgcgc agttcctcac cggctaccag aacgccgcct acgcgacgcg
      601 ctaccaggtg ctggtcgggc gcgtgcgcat ctccgagcag gacaaggtcg gcggcacgaa
      661 actcgccgag gcggtggcac gcaactacgc caagctgctc gcctacaagg acgagtacga
      721 ggtggcgcgc ctgtacgcct cgggcgaatt catgcgcaag atcgcgggca tgttcgaagg
      781 cgactaccgg ctgaaattcc atctcgcgcc gccgctgctc gccaagcccg acccggcgac
      841 gggcgagccg cgcaagatgc agttcgggcc atggatgctg cgcgtcttcg gcctgctcgc
      901 caggctgcgc tggctgcgcg gcacgccgct ggacccattc ggcgggacgc aggagcggcg
      961 catggagcgg gctctgatcg aggaatacga aatggcggtc gacgcgctgc tgcagggact
     1021 ggcgcgcgac aacctggcgc tggcggtgga gatcgcctcg attcccgaat cgatccgcgg
     1081 ctacggccac atcaaggcga agagcgtgga ggcggcgcgc aacaagcagt ccgcgctgct
     1141 ggcgcgcttc caggctgcgc cggtgagggc cgcagcctag gcagcggggt cagaccccga
     1201 tcgtcagccc ctgctgcgga tgaagtcgcg gaccctgggg tagatcaggg tgcgccagcg
     1261 ccggccgctg aagatgccgt agtggcccac ctttggcgcg aggtagtgct ggcgccgctc
     1321 gcgcgggatg ttcaggcaca gcaggtgggc ggactcggtc tgcccgttgc cggaaatatc
     1381 gtccagctcg ccctcgatgg tgagcagcgc cgtgtcgcgg atcagctgcg ggcgcaccag
     1441 gcggtcgcgg atgtagaggc gccctttggg cagggcgaat tcctggaaca ccgccttcac
     1501 ggtgtcgagg tagtactcgg cgggcaggtc gatcaccgcg ttgtactcgt cgtagaactc
     1561 gcggtgacgg tcggccgagt cgtcgtcgcc ctcgaccagg tggttgaagt agtcccagtg
     1621 cgcgttcagg tggcggtcgg ggttcatcgc cacgaatccc aggtgctgca ggaagcccgg
     1681 gtagacgcgt cgcatgtagc cgggatactt cgacggcacg cgctggatga cgttgcgctc
     1741 gaaccaggag tggggccgcg tcatcgccag gttgttgacg gcggtggggc tcttgcgcgc
     1801 atcgatcggc ccgcccatca tcaccatggt cctgggttgc ggcgccgtct tttcctcggc
     1861 cagcagcgcg atggcgcaca gcaccggcac cgtgggctgg cacaccgaga tcacgttcac
     1921 ctcgggcgac agcagcccga tgaattccct cacgtaatcc acgtagtcgg cgaggtggaa
     1981 cggaccggcg gcaagcggca ccatgcgcgc gtccacccag tcggtgaccc acacgtcgtg
     2041 ctcggggagc agggtcctta ccgtatcgcg cagcagcgtc gcatggtgcc ccgagagcgg
     2101 cgcgacgacc aggaccttgg gcaggccggc agcgcggggg gaatgcggcg tgaaatgcag
     2161 aagattgcag aacggcttgt cgagcgcgac ctcgatgtcg gcgccttcga tgcgccacgc
     2221 cggcttctcg tagcgctgcg tgatgcgcag gaacacgtcg ttcgaggcgg cgagctgctt
     2281 ggcgagcggt gcctgtgccc agggcatgaa agggttggcg aaccagcccc gctgcatctc
     2341 cgccagagcg cggtagggcg tgagccaggc gtgctgcgcc tcgtaaaggc tgtagagcat
     2401 cagcccttat agcagatttg ccgcagcgcg gcattggccg aatgcacacg gagaacgtat
     2461 ccttttcccg tgggattgtt cctcaagctg gttttcgcat tgctggggct ggcagggctc
     2521 gcgctcggcg gaggctatct ggtcctgcgc cagtccctgc cgcaggagga aggcgagatc
     2581 cgcctggcgg ggctgggcaa gccggtcgac gtgctgcgcg accgctacgg cgtgccgcac
     2641 atcacggcgg cgagcctcgg cgacgcggtg tacgcgctcg gcttcgtgca cgcccaggac
     2701 cgcctgtggc agatggaggt caaccgccgc actgccgcgg ggcggctggc cgaggtgttc
     2761 ggcgaggggg cgctcgaggc cgaccgtttc ctgcgcacgc tcggcgtgcg ccgcgcggcc
     2821 gaagcgaacc tgcagaggct cgacgcgcca actcgcgagc tgctcgagcg ctacgccgac
     2881 ggcgtcaacg cctttctcgc caccaagccc gtcctgcccg tcgaatactg gctcaccggc
     2941 gccgcggccg agccctggac acccgtcgac tccctcggct gggtgaagat gatggcctgg
     3001 gacctgggcg gcaactggcg caacgagttg ctgcgcatgc gcctcgcgaa gacgatgccc
     3061 aacgcgcgca tccacgaatt cctgccgccc taccccggcg aggcgccgcc ggagatcgcg
     3121 gacctgagga agctgtacgg cgggatggcg aaagaggggt cgaggctggc gcagttggaa
     3181 ggcgaaggcg gtgcctccga tttggccttt gaaaacgatc tccgtttttt tggcgacatg
     3241 aatctcgtcg ccaaaaaaac ggagaccatg gaaaatcaaa cgcaggcact gccttggttc
     3301 gacgccgacg cccagaccga aggccttggt tcaaataact gggtagtctc cggcgcccgc
     3361 agcgccaccg gcaagccgct gctcgccaac gatccgcacc tcgggctcag cgcgccggca
     3421 gtgtggtact tcgcgcacct caacgcgccg ggcttcgacg ccatcggcgc cacgctgccc
     3481 ggcgtcccgg cgatcgtgct cgggcgcaac gcgcatttcg cctggggctt caccaatacc
     3541 gggccggacg tgcaggacct ttacctcgag cgcctggacg gcgccggcaa ctacctcacg
     3601 cccgagggcc cgaaggcgtt cgtgctgcgc gacgaagtga tcaaggtgaa gggcggcgcc
     3661 gacgtgcgcc tgcaggtgcg cgcgtcgcgc cacgggccgg tgatctccga cgtgtcgcgc
     3721 cccgcgcagg acgcggcgcc gcgcggccac gtcatcgcct tccagtggac cgcgctgcgc
     3781 gaggacgaca tgaccatgca ggcggcggcg aagctcgcgc gggcgaggga ctggcccggc
     3841 ttcgtcaccg cgctgcgcga ctaccattcg ccgcagcaga acgtggtgtt cgccgacacc
     3901 gagggcaaca tcggtttcgt ggccgccggc cgcgtgccgg tgcgcaagcc cgagaacgat
     3961 ctgaaggggc tcgcgcccgc gcccggctgg ctggcgcagt acgactgggc agggttcatc
     4021 ccgttcgccg agctgccgct cgcctacaac cccgccgacg gcgtgctgat gagcgccaac
     4081 cacaagatca cgccgccggg ctacgggcat tacctcacca gcgagtggca gccgccttac
     4141 cgcgcgcggc gcatcgccga gctgctgggt gcgctgcgca ggcactccat cggcagcttc
     4201 gcgcgcatcc agggcgacgt ggtgtcgctg ccggcgaagg atctgctgcc gcgcctgggc
     4261 aaggtggtgc cgaaaagcga agccgggcgt cgcgcgctgt cgcggctcgc ggcctgggac
     4321 ggcgcgatgc tcgccgaccg gcccgaaccg ctcatcctcg cggcgtggtg gcgcgagttc
     4381 gcgcgcgcgg tgtacgccga cgaactgggc gaagccttcc gcgccaactg gctgacgcgc
     4441 gcgcagttcc tcaccaacgt gctcggcgac aaggaccgcc agggccgctg gtgcgacgac
     4501 gtgcgcacta agggcgccga gggctgcgac acgctgctcg ccgcgacgct cgaggccgcg
     4561 ctcgcggacc tgggcgggcg ctacggcgag gacatggacg cctggcgctg ggggactgcg
     4621 catcaggcgc ggcatgagca ccggcccttc gggcgggtgc gctggctcgc gccgctgttc
     4681 gacatcagcg taccgacacc cggcgacgcc tataccgtga acgtgggtcg cagccggctg
     4741 gaggatggcg cgcggcccta tgccaacacg cacgcggcca gcctgcgcgc gatctacgac
     4801 ctgtcgaacc cggacaactc gctgtacatc cattccggcg ggcagtcggg caacgtgctc
     4861 tccgcgcact acagggcctt cacccaggcg tgggccaacg gcgaatacat tccgatggtc
     4921 accgaccgcg ggaaaatcgc ggcgggcggc acgcgcaaac tgacgctcgc cccgcggcaa
     4981 taggcgcgag gccccttgcc gggctagcc
//
LOCUS       DASRIE010000134         6396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_669313, whole genome shotgun sequence.
ACCESSION   DASRIE010000134 DASRIE010000000
VERSION     DASRIE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669313"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFB53_05740"
     CDS             <1..219
                     /locus_tag="VFB53_05740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763844.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZN86272.1"
                     /translation="AAYTLWMYKRVIFGAVANAQVAALRDLNSREALVLGLLAAAVLF
                     MGVYPNPFTEVMHASVDGLLRHVAQPKL"
     gene            216..1712
                     /gene="nuoN"
                     /locus_tag="VFB53_05745"
     CDS             216..1712
                     /gene="nuoN"
                     /locus_tag="VFB53_05745"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZN86273.1"
                     /translation="MNLVALLDHIPVLPEVLLLAGACTLMIFDLYSKDASRRASFALA
                     QLTLLACLLTSLFILWKSGGKSFELFDHAFVADAMAGVLKVLAYGAVSLALAYARRYL
                     LDRGLLRGEFLALLLFALLGMMVMISANSFLTLYLGLELLSLCLYAMVALNRDSAASV
                     EAAMKYFVLGALASGLLLYGMSMIYGATGSLVIPEVAQRAARIAAESGSSRMLLVFGL
                     VFLVSGLAFKLGVVPFHMWIPDVYQGASTAVTLVISTAPKLAAFAMAMRLLVNGLPAL
                     AVDWQQMLAFLAVLSMAIGNLTAIAQTNLKRMLAYSTIAHMGFMLLGLMSGVVGGNQL
                     NAVDAYGAAMFYTVIYVAMSLGAFGVLLLLSRAGIECERLEDMKGLNRRDPWLAFLML
                     VLMFSLAGVPPTAGFYAKFAVLSAAVQAGQIAIAVAAVVFSLIGAFYYLRVVKLMYFD
                     EPAAGVDAAGQRGAARVLLAANSLALLAIGILPQTLMYVCLEAIRVSL"
     gene            1712..2242
                     /locus_tag="VFB53_05750"
     CDS             1712..2242
                     /locus_tag="VFB53_05750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN86274.1"
                     /translation="MSRDLAESFLEGEVVFSGNLVKLHRDRVRLPDGSEAVREYIRHP
                     GAVAVLALLDDGQVVLERQFRYPHGKVFIEIPAGKRDPGEAGEACARRELQEETGYRA
                     RDWRRLGIIHNAIAYSDEGIELWLASGLEAGRAKLEAGEFLEVFTLPLAEARGMVRDG
                     RITDAKTVAGLLWPRA"
     gene            complement(2239..5073)
                     /locus_tag="VFB53_05755"
     CDS             complement(2239..5073)
                     /locus_tag="VFB53_05755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZN86275.1"
                     /translation="MTATASAPAIAPQDICRDALLEKYAKGAETSIDEVRRRVARALA
                     QAEPEAKRGAWERRFLQALEAGFIPAGRINSAAGVQLQATLINCFVQPVGDSISEVVD
                     GRPGIYTALQEAAETMRRGGGVGYDFSSIRPKGAEVRGTRSRASGPVSYMRVFDRSCE
                     TVESAGARRGAQMGVLRCDHPDVEEFIHAKDRGDLANFNVSVGVTDAFMRAVEEDQDV
                     ELVHKAKPWPAPGVRQRDDGLWVYRKVPARTLWDQIMRSTYDHAEPGILFLDRMNRDN
                     NLNYCETIEATNPCAEQPLPPYGCCCLGSIDVTRFVRDAFAASAAFDFDGFGKVVEVA
                     VRMLDNVLEVTAWPLPAQLREAMAKRRVGLGFTGLGDALIMLRLHYDSDAARRMAARI
                     AEAMRDRAYLASAELAQERGAFPLFNADLYLSGSSFAARLPEEVKAKIRAQGVRNSHL
                     LSIAPTGTISLAFADNASNGIEPPFSWTYTRKKRMADGRLQEFPVEDHAWRRYKAAGG
                     NMQELPHYFVTALELSAAAHEQMVAAVAPYIDTSISKTVNVPEDYPYGEFKDLYLAAW
                     KSGLKGLATYRPNKVLGSVLSVDKAPADFVEDDVNRRIRIKSLPEPVLASLRWPGRPA
                     FAAGNLSWTYMVEHPRHHFAVFVGEAENGSPHAFEVWVNGSEQPRGLGAVAKTLSMDM
                     RANDPGWLRLKLDTLAKTTGDDGFDLAFPPHGEVARMPSVVSAFAQLVRWRVRQLGGL
                     SGEGQSDAFAASVTPVVDAMFSLKEPKTGTDGTLSWTVDVHNPRTGDDFVLGLKEIAL
                     PDGTTRPYSLWLSGEYPRALDGLCKLLSLDMRVVDPAWIGMKLRKLLDFPEPLGDFLA
                     FVPGSKRQQTWPSTVAYVARLVLHRYAMLGLLDEEGLPLQRMGVLQAPLRGGAPLLQG
                     AKCPECGSHTLIRKDGCDWCTACGYEGVCG"
     gene            complement(5157..5876)
                     /locus_tag="VFB53_05760"
     CDS             complement(5157..5876)
                     /locus_tag="VFB53_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN86276.1"
                     /translation="MIQRTIEKDDLLTKMRVREVLLFGLIAMTAVLANLPAETIAELG
                     INHDYLIAGLGCAVVIGLFLYLKFFFFLAVALLIVGANMPEQIAERFSISKIPLILAL
                     VALVGISLINYVVKLLPTGLEPKPKEKSAEGVRAMFYAIEKNNVVYGQKVLSMNFDAN
                     LHHDNGYTPLAYAAMKGNPQMVELFLRNGADPAQTTREGDTPVELALRMGHNEVADAL
                     KRARQQREAAPAAPAPAQARA"
     gene            5972..>6396
                     /locus_tag="VFB53_05765"
     CDS             5972..>6396
                     /locus_tag="VFB53_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZN86277.1"
                     /translation="MSKAFVKEPDAGEDPAPEAAPEQPLPRNYITPAGYARLKAELKQ
                     LVEVERPEVVRTVSWAASNGDRSENGDYLYGKKRLREIDRRVRFLIRRLEGAAVVDNA
                     GRDDAQVFFGARVTLRAPGGAARAVCIVGADEVDPARGRV"
BASE COUNT          908 a         2171 c         2263 g         1054 t
ORIGIN      
        1 gctgcctaca cgctgtggat gtacaagcgc gtcatcttcg gcgcagtggc caacgcccag
       61 gtcgcggcgc tcagggacct gaattcccgc gaggcgctgg tgctcgggct gctcgccgcg
      121 gcggttctgt tcatgggcgt ctaccccaat cccttcaccg aggtgatgca cgcctcggtg
      181 gacgggctgc tgcgccacgt ggcgcagccc aagctatgaa cctcgtcgcc ctgctggacc
      241 acatcccggt gctgcccgag gtgctgctgc tggccggcgc ctgcacgctg atgatcttcg
      301 acctgtactc gaaagacgcg tcgcgccgcg ccagcttcgc cctggcgcag ctgaccctgc
      361 tcgcctgcct gctgacctcg ctgttcatcc tgtggaagag cggcggcaag agcttcgagc
      421 tgttcgacca cgccttcgtc gccgacgcca tggcgggagt cctcaaggtc ctcgcctacg
      481 gcgcggtgtc gctggcgctg gcctatgcgc gccgctacct gctggaccgc ggcctgctgc
      541 gcggcgagtt cctcgcgctg ctgttgttcg cgctgctcgg catgatggtg atgatcagcg
      601 ccaacagctt cctcacgctg tacctggggc tggagctgct gtcgctgtgc ctgtacgcca
      661 tggtggcgct gaaccgggat tcggcggcct cggtggaggc ggcgatgaag tacttcgtgc
      721 tgggcgcgct cgcctcgggc ctgctgctgt acggcatgtc gatgatctac ggcgccaccg
      781 gctcgctggt gatccccgag gtggcgcagc gcgcggcgcg catcgccgcc gagtcgggca
      841 gctcgcgcat gctgctggtg ttcggcctgg tgttcctggt gtccggtctc gccttcaagc
      901 tcggcgtggt gccgttccac atgtggatcc cggacgtcta ccagggcgcc agtaccgcgg
      961 tcaccctggt catcagcacc gcgcccaagc tcgccgcctt tgccatggcg atgcggctgc
     1021 tggtgaacgg attgccggcg ctggcggtgg actggcagca gatgctggcg ttcctggcgg
     1081 tgctgtcgat ggcgatcggc aacctcaccg ccatcgcgca aaccaacctc aagcgcatgc
     1141 tggcctattc gaccatcgcg cacatgggct tcatgctgct cgggctgatg tccggcgtgg
     1201 tgggcggcaa ccagctgaac gcggtcgacg cctacggcgc ggcgatgttc tacaccgtga
     1261 tctacgtcgc gatgtcgctg ggcgccttcg gcgtgctgct gctgctgtcg cgcgccggca
     1321 tcgagtgcga gcggctggag gacatgaagg gcctcaatcg gcgcgatccc tggctcgcct
     1381 tcctcatgct ggtgctgatg ttctcgctcg ccggcgtgcc gccgacggcg ggcttctacg
     1441 ccaagttcgc cgtgctctcg gcggcggtgc aggccgggca gatcgccatc gccgtggcgg
     1501 cggtggtgtt ctcgctgatc ggcgccttct actacctgcg cgtcgtcaag ctcatgtact
     1561 tcgacgagcc ggcggcgggc gtggacgcgg cggggcagcg gggcgccgcg cgcgtgctgc
     1621 tcgccgccaa cagccttgcg ctgctcgcca tcggcatcct gccgcagacg ctgatgtacg
     1681 tttgcctgga agccatcagg gtctcgctgt agtgtccagg gacctcgccg agtccttcct
     1741 cgagggcgag gtggtgttct ccggcaacct ggtgaagctg caccgcgacc gcgtgcgcct
     1801 gcccgacggc agcgaggcgg tgcgcgagta catccgccac cccggggcgg tggcggtcct
     1861 cgcgctgctg gacgacgggc aggtggtgct cgagcgccag ttccgttatc cccacggcaa
     1921 ggtgttcatc gagattccgg cgggcaagcg ggacccggga gaggcggggg aggcctgcgc
     1981 ccggcgcgaa ttgcaggaag agaccggcta ccgggcccgc gactggcgcc ggctcggcat
     2041 catccacaac gccatcgcct acagcgacga gggcatcgag ctgtggctgg cgagcgggct
     2101 ggaggcgggc cgggcgaagc tggaggcggg ggagttcctg gaggtgttca ccctgccgct
     2161 cgccgaagcg cgaggcatgg tgcgcgacgg acgcatcacc gacgccaaga ccgtcgccgg
     2221 actgctctgg ccgcgcgcct agccgcagac tccctcgtag ccgcaggcag tgcaccagtc
     2281 gcagccgtcc ttgcggatca gcgtatggct gccgcactcc gggcacttgg cgccctgcag
     2341 cagcggtgcg ccgccgcgca gcggcgcctg gagcacgccc atgcgctgca gcggcaggcc
     2401 ttcctcgtcg agcagcccca gcatggcgta acggtgcagc accagccgcg ccacgtaggc
     2461 cacggtggag ggccaggtct gctgccgctt cgaccccggc acgaaggcga ggaagtcgcc
     2521 gagcggctcg gggaaatcga ggagcttcct gagcttcatc ccgatccagg ccgggtccac
     2581 gacgcgcatg tcgagcgaca gcagcttgca caggccgtcg agcgccctcg ggtactctcc
     2641 cgagagccac agcgagtagg gccgggtggt gccgtcgggc agcgcgattt ccttcaggcc
     2701 gagcacgaaa tcgtccccgg tgcgcgggtt gtgcacgtcc acggtccagg acagcgtgcc
     2761 gtcggtgccg gtcttgggct ccttcaggct gaacatcgca tccaccaccg gcgtgacgct
     2821 cgccgcgaag gcgtccgact gcccttcgcc ggacaggccg ccgagctgcc gcacgcgcca
     2881 gcgcaccagc tgcgcgaacg cggacaccac cgagggcatg cgcgcgactt cgccgtgcgg
     2941 cgggaaggcc aggtcgaagc cgtcgtcgcc ggtggtcttc gccagcgtgt cgagcttcag
     3001 gcgcagccag cccgggtcgt tggcgcgcat gtccatggac agggtcttgg cgaccgcgcc
     3061 caggccgcgc ggctgctcgc tgccgttcac ccacacctcg aaggcgtgcg ggctgccgtt
     3121 ctcggcttcg ccgacgaaca ccgcgaagtg gtgccgcggg tgctccacca tgtaggtcca
     3181 ggacagattg ccggcggcga acgcgggccg ccccggccag cgcaggctgg cgagcaccgg
     3241 ctcgggcagg gacttgatgc ggatgcggcg gttgacgtcg tcctcgacga agtccgccgg
     3301 cgccttgtcc accgacagca ccgagccgag caccttgttc ggccgatagg tggcgagtcc
     3361 cttgaggccc gatttccacg ccgcgaggta caggtccttg aactcgccgt acgggtagtc
     3421 ctccggcacg ttcaccgtct tggagatgct ggtgtcgatg tagggcgcca ccgcggccac
     3481 catctgctcg tgcgccgcgg cggagagctc cagcgcggtg acgaaatagt gcgggagctc
     3541 ctgcatgttt ccacccgccg ccttgtagcg gcgccaggcg tggtcctcga ccgggaattc
     3601 ctgcagcctg ccgtcggcca tgcgcttctt gcgcgtgtag gtccaggaaa agggcggctc
     3661 gatgccattg gaggcgttgt cggcgaaggc gaggctgatg gtgccggtgg gcgcgatcga
     3721 cagcaggtgc gaattgcgca ccccctgcgc gcggatcttc gctttcacct cctcgggcag
     3781 gcgcgcggcg aagctcgaac ccgacaggta caggtcggcg ttgaacagcg ggaaggcgcc
     3841 gcgctcctgc gccagctcgg cggaggcgag gtaggcgcgg tcgcgcatcg cctcggcgat
     3901 gcgcgccgcc atgcggcgcg cggcatcgct gtcgtagtgc aggcgcagca tgatgagagc
     3961 gtcccccagg ccggtgaagc ccagccccac gcggcgcttg gccatcgcct cgcgtaattg
     4021 cgccggcagc ggccacgccg tgacctcgag cacgttgtcg agcatgcgca ccgcgacctc
     4081 gaccaccttg ccgaagccgt cgaaatcgaa ggccgccgac gcggcgaagg cgtcgcgcac
     4141 gaagcgcgtc acgtcgatcg agcccaggca gcagcagccg tagggcggca gcggctgctc
     4201 ggcgcaggga ttggtggcct cgatggtctc gcagtaattc aggttgttgt cgcggttcat
     4261 gcggtcgagg aacaggatcc ccggctcggc atggtcgtag gtcgagcgca tgatctggtc
     4321 ccacagcgtg cgcgccggga ccttgcggta cacccacagt ccgtcgtcgc gctgccgcac
     4381 gccgggcgcc ggccagggtt tggccttgtg caccagctcc acgtcctggt cctcttccac
     4441 ggcgcgcatg aacgcgtcgg tgacgccgac cgagacgttg aaattggcaa ggtcgccccg
     4501 gtccttggcg tggatgaact cctccacgtc cgggtggtcg cagcgcagca cccccatctg
     4561 cgcgccgcgg cgcgcgccgg ccgactccac cgtctcgcag gaacggtcga acacgcgcat
     4621 gtaggacacc ggcccgctgg cgcgcgagcg cgtgcctctc acttcggcgc ccttgggccg
     4681 gatggaggag aagtcgtatc ccacgccgcc gccgcgccgc atggtctcgg cggcttcctg
     4741 cagcgccgtg tagatgccgg ggcggccgtc caccacctcc gagatcgagt cgcccaccgg
     4801 ctgcacgaag cagttgatca gggtcgcctg caactgcacg ccggcggcgg aattgatgcg
     4861 cccggcgggg atgaagccgg cctccagcgc ctgcaggaag cgccgctccc aggcgccgcg
     4921 tttcgcttcg ggctcggcct gggcgagggc gcgcgccacc cgccggcgca cctcgtcgat
     4981 ggaggtttcc gcgcccttgg cgtacttctc cagcagcgcg tcgcggcaga tgtcctgcgg
     5041 cgcgatggcg ggcgcggagg cggtggcggt caagtcgctc acagtcggag agctgcagta
     5101 aactgcttat atatacagta ttgtggcgcc aaagcgccgg tcccgcaagc gccgtttcag
     5161 gcgcgcgcct gggcgggcgc cggcgcggcc ggcgcggctt cgcgctgctg gcgcgcgcgc
     5221 ttcagggcat cggccacctc gttgtggccc atgcgcagcg ccagctcgac cggggtgtcg
     5281 ccctcgcgcg tggtctgcgc cgggtcggcg ccgttgcgca ggaacagctc caccatctgc
     5341 gggttgccct tcatcgccgc gtaggcaagc ggcgtatagc cgttgtcgtg gtgcaggttc
     5401 gcatcgaagt tcatcgacag caccttctgc ccgtacacca cgttgttctt ctcgatggcg
     5461 tagaacatgg cgcgcacgcc ctcggcgctc ttttccttcg gcttgggctc cagccccgtg
     5521 ggcagcagct tcaccacgta gttgatcagc gaaatgccca ccagcgccac cagggcgagg
     5581 atcaggggga tcttggagat gctgaagcgc tcggcgatct gctcgggcat gttggcgccc
     5641 acgatcagca gcgcgacggc gaggaagaag aagaatttca ggtacaggaa caggccgatc
     5701 accaccgcgc agccgagccc cgcgatcagg tagtcgtggt tgatgcccag ctcggcgatg
     5761 gtctcggcgg gcaggttggc gagcaccgcg gtcatggcga tcaggccgaa cagcaggacc
     5821 tcgcgtacgc gcatcttggt cagcaggtcg tctttttcga tcgttctctg gatcaccgcc
     5881 acggcttccc cccggggatt cttcgacgca ggcggaattc tcggcggtcc atcgcggcct
     5941 tgcaaggggc gaacgcgtag aatgcgcacc catgtcgaag gcgttcgtca aggagcctga
     6001 cgcgggagag gacccggcgc cggaggccgc gccggagcag ccgctgccca ggaactacat
     6061 cacgccggcg ggatacgcgc gcctcaaggc cgaactgaag cagctggtcg aggtggagcg
     6121 gccggaagtg gtgcgcacgg tgtcatgggc ggcgtccaac ggcgaccgct cggagaacgg
     6181 cgactacctg tacggcaaga agcgcctgcg cgagatcgac cggcgcgtgc gcttcctcat
     6241 ccggcggctg gaaggcgccg cggtggtgga caacgccggg cgcgacgacg cgcaggtgtt
     6301 cttcggcgcg cgcgtgacgc tgcgcgcgcc gggcggcgcg gcgcgcgcgg tgtgcatcgt
     6361 cggcgcggac gaagtcgacc cggcgcgcgg ccgggt
//
LOCUS       DASRIE010000135         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1960438, whole genome shotgun sequence.
ACCESSION   DASRIE010000135 DASRIE010000000
VERSION     DASRIE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960438"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="dctP"
                     /locus_tag="VFB53_05770"
     CDS             <1..826
                     /gene="dctP"
                     /locus_tag="VFB53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZN86278.1"
                     /translation="LRLVRSGQVDVGSAPLGMVAGDAPLLDGMDLAGLAPDIQTARRI
                     ADALTPHVNRDLERIGIRMVATLAFSKQVMFCRKAVPSLTDLQGMKVRAAGPSLGDLV
                     RSFNGQPVSVNFGEVYTALERGTIDCAVTGTGGGNSAKWTEVTSHLVNYPMQWAVAGY
                     YVNLAWWNKLDAPVRALLEKTFQEITDKQWALGSEITEDGLACNFGNAPSCKIHTLLK
                     KPLTEVKAGDAASAAAVRKQLVEVVLPNWVKRCGERCGQIYNDVVAPISGVKYTPR"
     gene            823..1401
                     /locus_tag="VFB53_05775"
     CDS             823..1401
                     /locus_tag="VFB53_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZN86279.1"
                     /translation="MSGPWRALERASQLALSIAGWCFLAMTLLICFDIVARRLLGFSS
                     GATSELSGYLLAIGMSWGLAAALIERAHVRIDILVQKLPLAARAWLHFIALAVLLGVA
                     GFFAWGAAALALDSWQLRATDVSQLSTPLVVPQALWAAGMALFLLAGLGLAARTLRAL
                     AAGRPAEVERLLVPRHYEEEVAETLEATRTVR"
     gene            1398..>2452
                     /locus_tag="VFB53_05780"
     CDS             1398..>2452
                     /locus_tag="VFB53_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN86280.1"
                     /translation="MIASAFALILGLVIALASLLGAGSGAAASGSVPLWAFGIVLAVF
                     LLLFAGGIYIGAALAALALIGGFTLADRPFWNFMGEILWSPSTNFVLVAVPLFLVMGE
                     VMLRAGLSGRLFSALNVWFGRLPGGLLHTNILSCSVFSAIAGSSVATAATMGSVALPH
                     FERTRYPPPLVLGSLAAGGALGNLIPPGITFIIYGMLTETSVGALYLAAVGPSLLVVG
                     LFMLLIVWLTWKRSFAAEGALPPDWRARLASLADLVPTAILIALVLGTIYTGLATPTE
                     SAAMGVVGAIVLGIIDRRLTLRMLHEALRSAALTTSMIALILFGAYFLNYIMSSLGVP
                     QLLAGFLTGLPLPAWAL"
BASE COUNT          296 a          868 c          853 g          435 t
ORIGIN      
        1 gctgcggctg gtgcgctcgg ggcaggtgga cgtcggctcg gcgccgctcg gcatggtggc
       61 gggggacgca ccgctcctcg acggcatgga cctcgcgggc ctcgcgcccg acatccagac
      121 cgcccggcgc atcgccgacg cgctgacccc gcacgtcaac cgcgacctcg aacgcatcgg
      181 catccgcatg gtcgctacgc tcgcgttctc caagcaggtg atgttctgcc gcaaggccgt
      241 gccgtcgctg accgacctgc agggcatgaa ggtgcgcgcc gccgggccgt cgctcggcga
      301 cctggtgcgc tcgttcaacg gccagccggt ttccgtcaac ttcggcgagg tctacaccgc
      361 gctcgagcgg ggcacgatcg actgcgcggt caccggcacc ggcggcggca actccgccaa
      421 gtggaccgag gtgaccagcc atctcgtgaa ctacccgatg caatgggccg tggccgggta
      481 ttacgtgaac ctcgcctggt ggaacaagct cgacgcgccg gtgcgcgcgc tgctcgagaa
      541 gaccttccag gaaatcaccg acaagcagtg ggcgctcggg tccgagatca ccgaggacgg
      601 cctggcctgc aacttcggca acgccccgag ctgcaagatc cacacgctgc tgaagaagcc
      661 gctcaccgag gtcaaggcgg gcgacgcggc gagcgccgcg gcggtgcgca agcagctggt
      721 cgaggtggtg ctgcccaact gggtcaagcg ctgcggcgag cgttgcggcc agatctacaa
      781 cgacgtggtg gcgccgatct cgggcgtcaa gtacacgccg cgatgagcgg tccgtggcgc
      841 gcgctcgagc gcgccagcca gctcgcgttg tcgattgcgg gctggtgctt cctcgcgatg
      901 acgctgctca tctgcttcga catcgtggcg cggcgcctgc tcggcttttc ctccggcgcg
      961 accagcgagc tctccggcta cctgctcgcc atcggcatgt cctgggggct ggccgcggcg
     1021 ctcatcgaac gcgcgcacgt gcgcatcgac atcctggtgc agaagctgcc gctcgcggcg
     1081 cgcgcctggc tgcatttcat cgcgcttgcg gtgctcctcg gcgtggccgg ctttttcgcg
     1141 tggggcgcgg cagcgctcgc gctcgattcc tggcagctgc gcgcgactga cgtctcgcag
     1201 ctcagcacgc cgctcgtggt gccccaggcg ctctgggcgg ccggcatggc gcttttcctg
     1261 ctggcggggc tcgggctcgc tgcgaggacg ctgcgcgcgc tcgccgcggg acgcccggcg
     1321 gaggtggagc ggctgctcgt gccacgccac tacgaggagg aagtggccga gacgctggaa
     1381 gccactcgca cggttagatg atcgcttcgg ccttcgcgct gatcctgggg ctggtgatcg
     1441 cgctcgccag cctgctcggc gcgggaagcg gcgccgccgc gtccggctcg gtgccgttat
     1501 gggcgttcgg catcgtgctg gcggtgttcc tgctcctttt cgccggcggg atctacatcg
     1561 gcgccgcgct ggccgcgctc gccctgatcg gcggcttcac gctcgcggat cggccgttct
     1621 ggaacttcat gggcgagatc ctgtggtcgc cttccaccaa cttcgtgctg gtggcggtgc
     1681 cgctctttct ggtgatgggc gaggtcatgc tgcgcgccgg cctttccggg cgcctcttca
     1741 gcgcgctcaa cgtctggttc ggccgcctgc cgggcggcct gctgcacacc aacatcctct
     1801 cgtgctccgt attctcggcc atcgctggct cgagcgtcgc caccgccgcc accatgggct
     1861 cggtggcgct gccgcacttc gagcgcacgc gctatccgcc gccgctggtg ctggggtcgc
     1921 ttgccgccgg cggcgcgctc ggcaacctga tcccgccggg catcacgttc atcatctacg
     1981 gcatgctgac ggagacctcg gttggcgcgc tctatctcgc cgccgtgggc ccgagcctgc
     2041 tggtggtcgg gcttttcatg ctgctcatcg tctggctcac ctggaagcgc agcttcgccg
     2101 ccgaaggcgc gctgccgcca gactggcgcg cgcgcctcgc gagcctggcg gacctggtgc
     2161 cgaccgcgat cctgatcgcg ctcgtgctgg ggaccatcta tacggggctc gccacgccga
     2221 ccgagtccgc ggcgatgggc gtggtcggcg cgatcgtgct cgggatcatc gaccggcgcc
     2281 ttacgctgcg catgctgcat gaggcgctgc gcagcgccgc gctcacgacc tcgatgatcg
     2341 cgctcatcct gttcggcgcg tacttcctca actacatcat gtcctcgctg ggcgtgccgc
     2401 agctgctggc gggcttcctc accgggctgc cgctgccggc gtgggcgctg at
//
LOCUS       DASRIE010000136         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1704610, whole genome shotgun sequence.
ACCESSION   DASRIE010000136 DASRIE010000000
VERSION     DASRIE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704610"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFB53_05785"
     CDS             complement(<1..895)
                     /locus_tag="VFB53_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZN86281.1"
                     /translation="MKPTLQFRLSQHLTLTPQLQQSIRLLQLSTVELNQEIEKMLMEN
                     PILEREDIEGDAQAAFVPLRDAPQSDEMRNAPAQDAPAPEEHAERHEDWLAEVAAPTW
                     RGDDEEEGERGFAAPDTPTLREHLNQQLSLTGIGERDRALVALLIDVLDDDGYLTQPL
                     EEIAALFSAEAEVDLEELGIALRHLQNFEPAGVGARTPAECLLLQLRADAALREHAAA
                     ALAIEIAEKHLPLLAGRDYARLKSLTGASEESLRAAQKLILGLNPRPGAAFARVETRY
                     MIPDVIVRKLKGVWRASLNPEA"
     gene            complement(923..1645)
                     /gene="lptB"
                     /locus_tag="VFB53_05790"
     CDS             complement(923..1645)
                     /gene="lptB"
                     /locus_tag="VFB53_05790"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZN86282.1"
                     /translation="MSQLRCERLRKRYKSRAVVQDVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLIAADAGRIHLDGRELTHLPIHARARLGISYLPQENSVFRKLTVEENVQAV
                     LELQGLPRAEIEARLQELLKDLNIAHLRKHTALSLSGGERRRLEIARALGTRPAFILL
                     DEPFAGVDPIAVLDIQRIIRFLKERSIGVLITDHNVRETLGICDRAYIINEGAVLASG
                     HPEEIVYNDSVRRVYLGEHFRM"
     gene            complement(1642..2169)
                     /gene="lptA"
                     /locus_tag="VFB53_05795"
     CDS             complement(1642..2169)
                     /gene="lptA"
                     /locus_tag="VFB53_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZN86283.1"
                     /translation="MGRRIAIAAAMAIAAMSAFSARAEKADREKPTQVEANRMTSDEA
                     RRISIFEGNVVLTKGTMQLRAERVVVRQDADGYQTASASGKPVRFRQKSDARAGQEGI
                     WLDGEALRVEIDEKNERVELHESARVTRDQDEVRGNYILYDQRSEFFSVSSAKGAPDS
                     RVRAVIQPKKPAVKP"
     gene            complement(2169..2738)
                     /gene="lptC"
                     /locus_tag="VFB53_05800"
     CDS             complement(2169..2738)
                     /gene="lptC"
                     /locus_tag="VFB53_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZN86284.1"
                     /translation="MNLSATRLFPLVLMLALALVTFWLERTVREEPAPAAQRSHHPDY
                     VVERFTITDFNRAGIAESALSAARMEHFPDDDSTDLQAPRVMQARPGQPRLTLAADRG
                     TLSQDGEELFLYDNVLVAREATAGLAEARMQTGFLHVVRARSLVRTDREVRISEGGRA
                     IIGRGMEYDNESRQLSLHAEVRGSFEAKQ"
     gene            complement(2735..3247)
                     /locus_tag="VFB53_05805"
     CDS             complement(2735..3247)
                     /locus_tag="VFB53_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HZN86285.1"
                     /translation="MAQVTADALERARRLRLMLFDVDGVLTDGRLWYGPGGEALQAFH
                     VLDGHGVKLLQQGGIAAGILSGRSSSAVALRAAELGVAHVLQGIEDKRPAFDGLLARL
                     GLQAAEAGYMGDDLPDLPVLARCGLACAPRSAHAEVRRGAHYVADAEGGCGAVREVCD
                     YVLRAQGRRP"
     gene            complement(3252..4280)
                     /locus_tag="VFB53_05810"
     CDS             complement(3252..4280)
                     /locus_tag="VFB53_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZN86286.1"
                     /translation="MIKAAPKRAQNQHGKAQNPHGASGSLELAKRVLQIEADAVRALI
                     DRVDERFLAAVEMIHHRTGRVVVSGIGKSGHIARKIASTFASTGTPAYFVHPAEASHG
                     DLGMIRAEDVFIAISNSGESAELLRIVPAIKREGAKLVAITGNADSPLAREADAHLDA
                     GVEKEACPLNLSPTASTTAALALGDALAIALLDARGFSAADFARSHPGGALRREALKH
                     VADVMHRGAEVPRVRENATLKQALDEMTRGGLGMTAVVDAQGRVKGIFTDGDLRRALK
                     KVAGLKAARIARVMTANPRTIAPQALATEAVALMDRHRISQLLVTGAAGELIGALNMN
                     DLLRAKVV"
     gene            4299..>4486
                     /locus_tag="VFB53_05815"
     CDS             4299..>4486
                     /locus_tag="VFB53_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN86287.1"
                     /translation="MDYTADSLPSVLILLATGVAAVVLCRTLRLPPLVGYLATGLMLG
                     PHALGLASDRETIHTLAE"
BASE COUNT          578 a         1604 c         1596 g          708 t
ORIGIN      
        1 tggcctcggg gttgaggctg gcgcgccaca cgcccttcaa cttgcgcacg atcacgtccg
       61 ggatcatgta gcgcgtctcg acgcgcgcaa aggcggcgcc ggggcgcggg ttgaggccga
      121 ggatcagctt ctgcgccgcg cgcagcgact cctcgctcgc gccggtgagg ctcttcaggc
      181 gcgcgtagtc ccggccggcg agcagcggca ggtgcttctc cgcgatctcg atggcgagcg
      241 cggcggccgc gtgctcgcgc agcgcggcat cggcgcgcag ctgcagcagc aggcattccg
      301 ccggcgtgcg cgcgcccacg ccggcgggct cgaagttctg caggtggcgc agcgcgatgc
      361 ccagctcctc gaggtccact tccgcctcgg cggaaaacag cgcggcgatc tcctccagcg
      421 gctgcgtcag gtagccgtcg tcgtccagga cgtcgatcag cagcgcgacc agcgcgcggt
      481 cgcgctcgcc gatgccggtg aggctgagct gctggttgag gtgctcgcgc agcgtcggcg
      541 tgtccggcgc ggcgaagccg cgctcgcctt cctcctcgtc gtcgccgcgc caggtcggcg
      601 ccgcgacctc ggccagccag tcctcgtgcc gctcggcatg ctcctcgggc gccggggcgt
      661 cctgcgccgg cgcattgcgc atctcgtcgc tctgcggcgc gtcgcgcagc ggcacgaagg
      721 ccgcctgcgc atcgccctcg atgtcctcgc gctcgaggat cgggttctcc atcagcatct
      781 tctcgatctc ctggttcagc tcgaccgtgg agagctgcag cagtcggatc gattgctgca
      841 gctgcggcgt cagcgtcaga tgctgcgaga gccggaactg cagggtgggc ttcatgggca
      901 ggagaggtcc gccggggggc ggctacatgc ggaaatgctc gccgagatat acccggcgca
      961 cgctgtcatt atagacgatc tcctccgggt ggcccgaggc gagcaccgcg ccctcgttga
     1021 tgatataggc gcggtcgcag atgccgagcg tctcgcgcac gttgtggtcg gtgatcagca
     1081 cgccgatcga gcgctccttc aggaagcgga tgatgcgctg gatgtcgagc accgcgatcg
     1141 ggtcgacgcc ggcgaaaggc tcgtcgagca ggatgaaggc gggccgcgtg ccgagcgcgc
     1201 gcgcgatctc caggcggcgg cgctcgccgc cggagagcga cagcgcggtg tgcttgcgca
     1261 ggtgcgcgat gttgagatcc ttcagcagct cctgcaggcg cgcctcgatc tcggcgcgcg
     1321 gcaggccctg cagctcgagc accgcctgca cgttctcctc caccgtgagc ttgcggaaca
     1381 ccgaattctc ctgcggcagg taggagatgc cgaggcgcgc acgcgcgtgg atcggcaggt
     1441 gcgtgagctc gcgcccgtcg aggtggatgc ggccggcgtc ggcggcgatc aggccgacga
     1501 tcatgtagaa gcaggtggtc ttgccggcgc cgttgggccc cagcaggccg accacctcgc
     1561 cgctggagac ctcgagcgag acgtcctgca ccacggcgcg cgacttgtag cgcttgcgca
     1621 ggcgctcgca gcgcagctgg ctcatggctt gaccgccggc ttcttcggct ggatcaccgc
     1681 ccgcacgcgc gaatcgggcg cccccttggc gctcgacacg gagaagaact cggagcgctg
     1741 gtcgtacagg atgtagttgc cgcgcacctc gtcctggtcg cgcgtgacgc gcgcgctctc
     1801 gtgcagctcg acgcgttcgt tcttctcgtc gatctcgacg cgcagcgcct cgccgtcgag
     1861 ccagatgccc tcctggccgg cgcgcgcgtc gctcttctgg cggaagcgca ccggcttgcc
     1921 gctggcgctg gcggtctggt agccgtcggc gtcctggcgc accaccacgc gctcggcgcg
     1981 cagctgcatg gtgcccttgg tgagcaccac gttgccctcg aagatgctga tgcggcgcgc
     2041 ttcgtcggag gtcatccggt tcgcttcgac ctgcgtgggt ttctcccgat ctgccttttc
     2101 ggccctggcc gaaaaggcag acatggctgc aatcgccatg gccgcagcga tggcgatgcg
     2161 ccggcccatc actgtttcgc ctcgaagctt ccccgcacct cggcgtgcag cgacagctgg
     2221 cgcgattcgt tgtcgtactc catgccgcgg ccgatgatgg cgcgcccccc ctcgctgatg
     2281 cgcacctcgc ggtcggtgcg caccagcgag cgcgcgcgca ccacgtgcag gaagccggtc
     2341 tgcatgcgcg cctcggcgag gccggcggtg gcttcgcgcg ccaccagcac gttgtcgtac
     2401 aggaacaact cctcgccgtc ctgcgacagc gtgccgcggt ccgccgccag ggtgaggcgc
     2461 ggctgcccgg gcctggcctg catcacgcgc ggcgcctgca agtcggtgga gtcgtcgtcc
     2521 gggaagtgct ccatcctggc ggccgacagc gccgactcgg cgatgcccgc gcggttgaaa
     2581 tcggtgatcg tgaagcgctc gacgacatag tccgggtggt ggctgcgctg cgccgcgggc
     2641 gccggctcct cgcgcaccgt gcgctcgagc cagaaggtga ccagcgcgag cgccagcatc
     2701 agcacgagcg ggaacaggcg cgtcgccgac aggttcacgg ccggcggccc tgcgcgcgca
     2761 gcacgtagtc gcagacttcg cgcaccgcgc cgcagccgcc ttcggcatcg gcgacgtagt
     2821 gcgcgccgcg gcgcacctcg gcgtgcgccg agcgcggcgc gcaggccagg ccgcagcgcg
     2881 cgagcaccgg caggtccggc aggtcgtcgc ccatgtagcc cgcctcggcg gcctgcagcc
     2941 ccaggcgcgc gagcaggccg tcgaacgcgg ggcgcttgtc ctcgatgccc tgcagcacgt
     3001 gcgccacgcc gagctcggcg gcgcgcagcg ccaccgcgct cgagctgcgc cccgacagga
     3061 tgccggcggc gatgccgcct tgctgcagca gcttcacgcc gtggccgtcg agcacgtgga
     3121 aggcctgcag cgcctcgccg ccgggcccgt accacaggcg gccgtcggtg agcacgccgt
     3181 cgacatcgaa cagcatgagc cgcagtcggc gcgcgcgctc gagcgcgtcg gcggtgacct
     3241 gcgccatcgg ctcagacgac ctttgcgcgc agcaggtcgt tcatgttgag cgcgccgatc
     3301 agttcgcccg cggcccccgt caccagcaac tggctgatgc ggtggcggtc catcagcgcc
     3361 accgcctcgg tggcaagcgc ctgcggcgcg atggtgcgcg ggttggcggt catcacccgc
     3421 gcgatgcgcg ccgccttcag gccggcgacc ttcttcagcg cgcggcgcag gtcgccgtcg
     3481 gtgaagatgc ccttgacgcg gccctgcgcg tccaccacgg cggtcatgcc cagtccgccg
     3541 cgcgtcattt cgtcgagcgc ctgcttgagc gtggcgttct cgcgcaccct gggcacttcg
     3601 gcgccgcgat gcatcacgtc ggccacgtgc ttgagcgcct cgcggcgcag cgcgccgccc
     3661 ggatgcgagc gcgcgaaatc ggcggcggaa aagccgcgcg catcgagcag cgcgatggcg
     3721 agcgcgtcgc ccagcgccag cgccgccgtg gtgctggcgg tgggcgagag attcagcggg
     3781 caggcctctt tttccacgcc ggcgtccagg tgcgcgtcgg cctcgcgcgc cagcggcgag
     3841 tcggcgttgc cggtgatcgc caccagcttg gccccttcgc gcttgatcgc cggcacgatg
     3901 cgcagcagtt ccgcgctctc gcccgaattg gagatggcaa tgaacacgtc ctcggcgcgg
     3961 atcatgccga ggtcgccgtg gctcgcctcg gccgggtgca cgaagtaggc cggcgtgccg
     4021 gtgctggcga aggtcgaggc gatcttgcgc gcgatgtgcc cggacttgcc gatgccgctc
     4081 accacgacgc ggccggtacg gtggtggatc atctcgaccg cggcgagaaa gcgctcgtcg
     4141 acgcggtcga tcagcgcgcg caccgcgtcg gcctcgatct gcagcacccg cttcgccagc
     4201 tcgagcgaac ccgacgcgcc gtgcggattc tgcgccttgc cgtgctgatt ctgcgctcgc
     4261 ttgggcgctg cctttatcat tggcgcggag tatagcccat ggactacacc gccgattccc
     4321 tgccgtcagt gctgattctg ctcgccaccg gcgtcgcggc ggtggtgctg tgccgcacct
     4381 tgcgcctgcc gccgctggtc ggctacctgg cgaccgggct gatgctcggg ccgcacgcgc
     4441 tcggcctcgc cagcgaccgc gagacgatcc atacgctggc cgagtt
//
LOCUS       DASRIE010000137         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_144667, whole genome shotgun sequence.
ACCESSION   DASRIE010000137 DASRIE010000000
VERSION     DASRIE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144667"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="mbhE"
                     /locus_tag="VFB53_05820"
     CDS             <1..781
                     /gene="mbhE"
                     /locus_tag="VFB53_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HZN86288.1"
                     /translation="AWTSEGARDARPRRLRDLALALSCGAGVAALAYAIMTRPLPETI
                     SSYFMQNAWPLGGGSNVVNVILVDFRGFDTLGEVSVLAVVGITVYALLRRFRPARESL
                     DTPPRAAAVQGAAEDLLVPAVIMRLMFPVIGLLAGYLLLRGHNQPGGGFVAGLALAIA
                     VILQYMAGGTRWAEERLAIRPARWMGAGLLLGGATGAGAWLFSHAFLTSHTAHVQLPV
                     LGELHLPSAFLFDLGIFSVVVGATGLILIAIAHQSVRHPWN"
     gene            772..1116
                     /locus_tag="VFB53_05825"
     CDS             772..1116
                     /locus_tag="VFB53_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HZN86289.1"
                     /translation="MELIIALLIGTLAGSGVWLLLRPRTFQVIIGLSLLSYAVNLFIF
                     VMGWVRGGRPPFVEPGAAVDFSRFADPLPQALVLTAIVISFATTALLLVVLLAARGLT
                     GTDHVDGQEPGE"
     gene            1113..>2193
                     /locus_tag="VFB53_05830"
     CDS             1113..>2193
                     /locus_tag="VFB53_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HZN86290.1"
                     /translation="MSWSAHLPIVPIVLPLAAGALMLLLDERRERLKAAISMAALLVL
                     LAAAVALLSLADRPLAPVYALGSWPAPFGIVLVADRLAAAMVLLSGLLGLAATVFSLA
                     RWQRAGPRFHALLQFLLMGLNGAFLTGDLFNLFVFFELLLAASYGLALHGSGTARVRT
                     SLHYIVINLAASLLFLIGVSLIYGVTGTLNLAHLAQRIPLVAAEDRVLVEAGAAVLAI
                     AFLVKSGMWPLGFWLPSTYAAASPPAAALFSILTKVGFYAVLRLWLLLFGGGVGAELL
                     YLGGMATLAFGAIGMLASQELGRLAGYSLLVSSGTLLAAISRGDGAVTAAALFYLVAS
                     TLGIAAFFLLIELVQRAREPGADVLA"
BASE COUNT          215 a          799 c          782 g          397 t
ORIGIN      
        1 cgcgtggacg agcgagggcg cgcgcgacgc gcggccgcgg cggctgcgcg acctggcgct
       61 cgcgctgagc tgcggggcgg gggtggccgc cctcgcctac gcgatcatga cccgcccgct
      121 gcccgaaacc atctccagct acttcatgca gaacgcctgg ccgctcggcg gcggcagcaa
      181 cgtggtgaac gtgatcctgg tggacttccg cggcttcgac accctgggcg aggtttcggt
      241 gctcgccgtg gtcggcatca cggtctacgc gctgctgcgc cgcttccggc cggcgcgcga
      301 gagtctcgac acgccgccgc gcgcggcggc ggtgcagggc gcggctgaag acctgctggt
      361 accggcggtg atcatgcggc tcatgttccc ggtcatcggc ctgctcgccg gctatctcct
      421 gctgcgcggc cacaaccagc cgggcggcgg cttcgtcgcc ggcctggcgc tggcgatcgc
      481 cgtcatcctg cagtacatgg cgggcggcac gcgctgggcg gaggagcgcc tcgcgatccg
      541 gccggcgcgc tggatgggtg ccgggctgct gctcggcggc gccaccggcg ccggcgcgtg
      601 gctcttttcg catgcgttcc tcacctcgca taccgcgcac gtgcagctgc cggtgctcgg
      661 cgagctgcat ctgccgagcg cgttcctgtt cgatctcggc atcttctcgg tggtggtcgg
      721 cgccaccggg ctgatcctga tcgcgatcgc gcaccagtcg gtacgccatc catggaactg
      781 atcatcgcgc tgctcatcgg cacgctcgcc ggctcgggcg tgtggctgct gctgcggccg
      841 cgcaccttcc aggtgatcat cgggctttcg ctgctgtcct acgcggtcaa cctgttcatt
      901 ttcgtgatgg gatgggtgcg cggcgggcgc ccgccgttcg tcgagcccgg cgcggcggtg
      961 gatttctccc gcttcgccga cccgctgccg caggcgctgg tgctgaccgc gatcgtcatc
     1021 agcttcgcca ccaccgcgct gctgctggtc gtgctgctgg cggcgcgcgg actgaccggc
     1081 accgaccatg tcgacgggca ggagcccggc gagtgagctg gagcgctcac cttccgatcg
     1141 tgccgatcgt gctgccgctt gccgccggtg cgctgatgct gctgctcgac gagcggcgcg
     1201 agcggctcaa ggcggcgatc agcatggccg cgctcctcgt gctgctggcc gccgcggtcg
     1261 cgctgctgag cctcgccgac cggccgctcg cgccggtcta cgcgctcggc agctggccgg
     1321 cgccgttcgg catcgtgctg gtcgccgacc ggctggcggc cgccatggtg ctgctcagcg
     1381 gcctgctcgg cctggccgcg accgtgttct cgctcgcgcg ctggcagcgc gccgggccgc
     1441 gctttcacgc cctgctgcag ttcctgctga tgggcctgaa cggcgcgttc ctgaccggcg
     1501 acctgttcaa cctgttcgtg tttttcgagc tgttgctcgc ggcctcctac ggcctcgcgc
     1561 tgcacggctc cggcaccgcg cgcgtgcgca ccagcctgca ctacatcgtc atcaacctcg
     1621 cggcctcgct gctgttcctg atcggcgtga gcctgatcta cggcgtgacc ggcacgctca
     1681 acctggcgca tctcgcgcag cgcatcccgc tggtcgccgc ggaagaccgc gtgctggtgg
     1741 aggccggcgc cgcggtgctc gccatcgcgt tcctggtcaa gtcgggaatg tggccgctcg
     1801 gcttctggct gccgtcgacc tacgcggcgg cgagcccgcc cgccgccgcg ctgttctcga
     1861 tcctgaccaa ggtcggcttc tacgcggtgc tgcggctctg gctgctgctg ttcggcggcg
     1921 gcgtcggcgc cgagctgctc tacctcggcg gcatggcgac gctcgccttc ggcgcgatcg
     1981 gcatgctcgc ctcgcaggag ctcggacgcc tcgccggcta cagcctgctg gtctcgtcgg
     2041 gaacgctgct ggcggcgatc agccgcggcg acggcgcggt gaccgccgcc gcgctgttct
     2101 acctggtggc ctcgacgctc gggatcgcgg cgttctttct gctgatcgag ctggtgcagc
     2161 gcgcgcgcga gcccggcgcc gacgtgctgg cgg
//
LOCUS       DASRIE010000138         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1962111, whole genome shotgun sequence.
ACCESSION   DASRIE010000138 DASRIE010000000
VERSION     DASRIE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962111"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFB53_05835"
     CDS             <1..558
                     /locus_tag="VFB53_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN86291.1"
                     /translation="TRYEARACLQAHDFAGHRRMLDVGGNSGEFALQACRAHPGLAAT
                     VMDLPLVCEIGAEHVLPHEERGRIAFLPGDLRRDALPAGHDLISFKSVLHDWPEADAL
                     RFLDAAVAALAPGGTLLVFERQPLEVERHPPPFGLLPVLLFFRSYRPPGFYQQALQER
                     GLLDVRAREVLLDVPFLLLTGRKAA"
     gene            576..1388
                     /locus_tag="VFB53_05840"
     CDS             576..1388
                     /locus_tag="VFB53_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN86292.1"
                     /translation="MPPLIRPIVCACAALLAASAPAGAQEFDCMIEARQAVEIRSPVE
                     AVIEQVHVKRGDTVTRGQLLVTLAAGPERAQLELAQSRARMQGEIKSAEARLDIAQKK
                     AQRSAELFKQSFISANARDESQAELQLATEELRRVRETQKLNELEARRAYEVVAMRSI
                     RSPLAGVVVEVMLKPGEFGAITFKDPIMKLAQIDPLHVEVVLPVSQYGQVKAGQKASV
                     MPEAPVGGRYETRVALVDKVVDAASGTFGVRLELRNRKFEIPAGVRCRVQFQ"
     gene            complement(1399..>2104)
                     /locus_tag="VFB53_05845"
     CDS             complement(1399..>2104)
                     /locus_tag="VFB53_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86293.1"
                     /translation="GTCVDARGLEGEFWSVYGLRCTRAGWAGAGMRVGARAFLASATR
                     RAALVDAVRARAAGGDAVVIAVRSNAEAGALTGALAQAGLRFGVLRDTGGAEQRAVLD
                     GLEQPGAVALSLFPAQRGIARTASARVPLHLAVAEMHEAHRHVAQIAHAYAASSCEQF
                     VALEDEGVAARAGWLAVRRARAGRRGGELAPAAARRLAAAAQRGMERAAARQRRELRA
                     REQSQEDLLAFSGRAE"
BASE COUNT          284 a          803 c          754 g          263 t
ORIGIN      
        1 acgcgctacg aggcgcgcgc ctgcctgcag gcgcacgatt tcgccggcca ccggcgcatg
       61 ctggacgtcg gcggcaacag cggggagttc gcgctgcagg cctgccgcgc gcatcccggc
      121 ctcgccgcca cggtgatgga cctgccgctg gtctgcgaga tcggggccga gcacgtgctg
      181 ccgcacgagg agcgcggccg catcgccttc ctgcccggcg acctgcgccg ggacgcgctg
      241 ccggccgggc acgacctgat cagcttcaag tccgtgctgc atgactggcc ggaggcggac
      301 gccctgcgct tcctggatgc cgccgtcgcc gcgctcgcgc cggggggaac gctgctggtg
      361 ttcgagcgcc agccgctcga ggtcgaacgg catccgccgc cgttcggcct gctgccggtg
      421 ctgctgttct ttcgctccta ccggccgccc gggttctacc agcaggcgct gcaggagcgc
      481 ggactgctcg acgttcgcgc ccgcgaagtg ctgctcgacg ttcccttcct gctcctcacc
      541 ggcaggaaag cggcataatt gcgcccgttc gcccgatgcc gcccctgatc cgaccgatcg
      601 tctgcgcctg cgcggcgctg ctggccgcgt ccgcgcccgc cggcgcgcag gaattcgact
      661 gcatgatcga ggcgcgccag gcggtggaga tccgcagccc ggtggaagcc gtgatcgaac
      721 aggtccacgt caagcgcggc gacacggtca cgcgcgggca gctgctggtc acgctggccg
      781 ccggccccga gcgggcgcag ctcgagctgg cgcagtcgcg cgcgcgcatg cagggcgaga
      841 tcaagtccgc cgaggcgcgc ctggacatcg cgcagaagaa ggcgcagcgc agcgccgagc
      901 tgttcaagca gagcttcatc tccgccaacg cgcgcgacga gtcgcaggcc gagctgcagc
      961 tggccaccga ggaattgcgc cgcgtgcgcg aaacgcagaa gctgaacgag ctggaggcgc
     1021 gccgcgccta cgaggtcgtg gcgatgcgca gcatccgcag cccgctcgcc ggcgtggtgg
     1081 tcgaggtgat gctcaagccg ggcgagttcg gcgccatcac cttcaaggac ccgatcatga
     1141 agctcgccca gatcgacccg ctgcacgtcg aggtggtgct gccggtgagc cagtacggac
     1201 aggtgaaggc cggccagaag gcgagcgtga tgcccgaggc gccggtcggc ggccgctacg
     1261 agacccgcgt cgcgctggtc gacaaggtgg tcgacgcggc gagcgggacc ttcggcgtgc
     1321 ggctcgagct gcgcaaccgc aagttcgaga tcccggccgg cgtgcgctgc cgggtgcaat
     1381 tccagtagcg ccgcgcgctc actccgcgcg tccggagaag gccagcaggt cctcctgcga
     1441 ctgctcgcgt gcgcgcagct cgcggcgctg gcgcgccgcg gcgcgctcca tgccgcgctg
     1501 tgcggcagcg gccaggcgcc gcgccgccgc cggcgccagc tccccgccgc gccgcccggc
     1561 gcgcgcgcgg cgcaccgcca gccacccggc acgcgccgcc acgccttcgt cctccagcgc
     1621 gacgaactgt tcgcaggaac tggcggcgta ggcgtgcgca atctgcgcga cgtggcggtg
     1681 cgcctcgtgc atttcggcca ccgcgagatg cagcggcacc cgggcgctgg ccgtgcgcgc
     1741 aataccgcgc tgcgccggga acagcgacag agcgaccgcg cccggctgct cgagtccgtc
     1801 caggaccgcc cgctgttccg cgccgcccgt gtcgcgcagc accccgaaac gcaagccggc
     1861 ctgcgccaac gctcccgtca gtgcgccggc ctccgcgttg gagcgcaccg cgatcaccac
     1921 ggcgtccccg cccgccgcgc gcgcgcgcac cgcgtcgacc agggccgcgc gccgcgtcgc
     1981 gctggcgagg aaggcgcgcg cgccgacgcg catgccggcg cccgcccagc cggcacgggt
     2041 gcagcgcagg ccgtacaccg accagaactc gccttccagc ccgcgtgcat ccacgcaggt
     2101 cccg
//
LOCUS       DASRIE010000139        10154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3807630, whole genome shotgun sequence.
ACCESSION   DASRIE010000139 DASRIE010000000
VERSION     DASRIE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3807630"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFB53_05850"
     CDS             complement(<1..925)
                     /locus_tag="VFB53_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN86294.1"
                     /translation="MTWQAAAWLLLGGSTALMFLGLPVAFAFLAINLVGAAIFLGGDA
                     GMMQLARNSVQSVTSFALTPIPLFILMGEILFHTGLAVKVIDGIERLVREVPGRLAVI
                     AVVAGTVFSAISGSTIATTAMLGSLMLPVMLSRGYHPTLATGPIMAIGAVDMLIPPSA
                     LTVLLGSLSGISISKLLVGGIVPGLILSVVFIGYIILRVKLEPALAPRTAFEEFRGWR
                     RLQPFLQYVLPLVSIFVVVVGAMIAGWATPTECAALGAFATLFLALLYRALSLANLVR
                     ALRGTAAISGMILFIIVGATTFSQILSFSGAS"
     gene            complement(936..1463)
                     /locus_tag="VFB53_05855"
     CDS             complement(936..1463)
                     /locus_tag="VFB53_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZN86295.1"
                     /translation="MRRLEEAYGRLLAGLALAACAVLFLMMAAICVDVLLRNVRLVPG
                     LLGLPWANEVTEYALYLVTVLTAPWLLRQGLHIRVDVLLRALPLRLAWACEWAVDLIA
                     LACCLAIGWYGLQAVLSSRAIGGMVVKVLAVPEWWLLAPLPAAFALLAIEVLFRMRRL
                     LLGERAPRTDAVTAA"
     gene            complement(1463..2425)
                     /gene="dctP"
                     /locus_tag="VFB53_05860"
     CDS             complement(1463..2425)
                     /gene="dctP"
                     /locus_tag="VFB53_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZN86296.1"
                     /translation="MNRTASAVLCLTLGLAAGAAAAQEVTLRVVSAFAENTTYVKNLL
                     PMIARLNAEGKGVLQLNFIGGPKAMPPFEVGNAVRTGVVDIGMTTGAFYTNIMPEADA
                     LKLTQMAGPELRRNGATDLINKVWNEKANMQYLCRVIDYTPFHLYLTKKVDKPDLTGL
                     KIRITPVYRDFFQALGATVMQTAPGEVYTALERGAVDGYGWPINGIFDFNWHEKTRFR
                     VDPGFYSAEVSLVMNLDKWKALSQRQKDFLMQHLVAHEADNVSWKKVNEDDIRRQAQA
                     GIQTITFDAATAKQYYDKAYDIAWTNLIKVSPQYGPQMRKLFSR"
     gene            complement(2448..4199)
                     /locus_tag="VFB53_05865"
     CDS             complement(2448..4199)
                     /locus_tag="VFB53_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HZN86297.1"
                     /translation="MAMRLHTRLRYAPAEVRVEKRADGTQLLRSPQPLGGHARAVGDW
                     LVQWYERAPERTFIAERKGDGWRRVSYRDALSDARRIGQALLNLGLDASRPVAILSDN
                     GVEHALLALGAMHAGVPVAPISPAYSLMSKDFAKLKAIFELLRPGLVFAAEPEKFAPA
                     LAAVGAVSTPVAKLLETNPGSSLELAFSRLQPDTVAKILFTSGSTGAPKGVLNTHRML
                     CANQQMLAQVWPFLEDRAQTLVDWLPWNHTFGGNFCFNLVLRNGGTLYVDGGKPAPAL
                     VETTVRNLREVSPTLYFNVPRGFDLLLPYLEKDAALRRNFFSNLDMLFYAGAALPQNL
                     WERLEALAIAEKQGELAMLSSWGSTETAPSAAAVHYPIERAGVIGLPNPGCELKLVPA
                     AGKLEVRVKGPHVTPGYLRRPDLTAEAFDEEGYYRIGDAMRFADPAAPEKGLVFDGRV
                     AEDFKLGTGTWVHVGALRVKLIAAGNPLIQDAVLTGHDRNEVGALLFLSPAAKDLSAE
                     ALRERLAAALRSLAAEGGSSTHPRRALVLAEPPSIDAGEITDKGYVNQRAVLERRAAL
                     VELLYADPPSAEVLGVK"
     gene            complement(4210..5106)
                     /locus_tag="VFB53_05870"
     CDS             complement(4210..5106)
                     /locus_tag="VFB53_05870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN86298.1"
                     /translation="MRVGFIGLGVMGAPIAARLARAGHAVKVNDLRKVDVAGAVWADS
                     PAAAVADAELAFTSLPGPAEVEAVAGLIGTALARHAAWFDLTTNSPSCVRRVHAALQP
                     KGVQFLDAPVSGGREGAHSGKLALWVGGDEGVYRRHEPVLRVIGDQPIYVGPIGAGSV
                     AKLAHNTASFTVQTALAEIFTLGVKAGVEPLALFRAIRQGATGRKRTFDRLAEQFLPG
                     KYDPPGFALRLAHKDLKLALELAQEVGVPMQVAQVAMREFEQALARGWAERDSRTPML
                     LQEERAGVAPRVPAEKLREVLD"
     gene            complement(5103..5435)
                     /locus_tag="VFB53_05875"
     CDS             complement(5103..5435)
                     /locus_tag="VFB53_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN86299.1"
                     /translation="MAATKSPPTTIAAVKDIVPKLAELTEKVLFGDVWARPGLSRRDR
                     SLATVAALIALHRPDQLKGHIWRALDNGVTQEELSELFTHLAFYAGWANGIGAAQVAK
                     QVYEERGG"
     gene            5533..6888
                     /locus_tag="VFB53_05880"
     CDS             5533..6888
                     /locus_tag="VFB53_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZN86300.1"
                     /translation="MKLESVIAVNRFGLGAAPGELAAASADPRAWLAKQASQPAPATP
                     EFGKLAHSSRALEEYPRWIASLRTAGTRSVEQTFRTHFGPIMLEELGARLAAAAATAT
                     PFQERLVWFWANHFTVSAEKPLVFALVGGFEREAIRPQVTGRFADLLLAAASHPAMIL
                     YLDNHLSVRKGWQPRRPSRNAAFPSPTGLNENLAREILELHTLGVNGGYTQADVTEFA
                     RVITGWTVRAQGERGFQFDPARHEPGPKTLLGKTYPEEGMAQGEAVLRDLAAHPATAL
                     HVATKLARHFIADDPPPAAVARIARAFRDTGGHLPSVYAALLDCPEAWERPLAKLKTP
                     VEYVVSCQRALPALRAARPQAMFFTLRGMGQRPFFAPSPQGWPDNTGAWAGADALWKR
                     IEWAGILAARLGTQVDPLALARESYGEAVGEATSLAIARAESRQQGLALWLASPEFQR
                     R"
     gene            6891..8042
                     /locus_tag="VFB53_05885"
     CDS             6891..8042
                     /locus_tag="VFB53_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN86301.1"
                     /translation="MATRRAALKFIGGMAACAGLPGLSLGAPSAGERRLVFVFLRGGM
                     DGLSAVPAHGDPQYAARRGALAVEAPLRLDATFGLSPYLAEMHKLYGAGELAVLHAVA
                     SPYRERSHFDAQNLLENGTARPFGRDTGWLNAALGAGARPRGLALGQSIPLVLRGPAQ
                     VSSWSPSALPVPDADLLERLAALYRGDPLLERSFAAAREAQGMMERRDAGGGVQAVLA
                     LAKAAGEILGKPEGPRVASIDFGGWDTHINQLGEYSALTRNLRLLDRSVAALKASLGE
                     AWRHTAVLIVTEFGRTVAPNGSNGTDHGTAGAAFVAGGAVRGARVIADWPGLAERALH
                     EGRDLRPTLDLRALFKAALLAQLSLDEAVLEAQVFPDSRGVKPLEGLFA"
     gene            complement(8031..8900)
                     /locus_tag="VFB53_05890"
     CDS             complement(8031..8900)
                     /locus_tag="VFB53_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86302.1"
                     /translation="MSSPASALELAAAGAVDLVAAGSLEMGAHRPQDAAAIAAVLPAG
                     TPVYVNHLPRHRLEDSLEAIAALRAAGLEPVPHIAARRIAGRGELAAFLRAALVRKAL
                     ILGGDEPEARGPYADGAALIRSGLLEDCGLREIGLPGYPEGHPRIPQLALQQAFGEKL
                     ALAAGHGLSAYVVTQFCFAPGRIVEYCAALARGAPGVPVYVGLAGPASAAALLRYAQR
                     CGVSASLRALQAQGLNAVRLVTRTDPGEQLAALARYCAAHAACNVVGVHLFSFGGVTA
                     TAAWMNRAIASGK"
     gene            complement(8875..>10154)
                     /locus_tag="VFB53_05895"
     CDS             complement(8875..>10154)
                     /locus_tag="VFB53_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86303.1"
                     /translation="LRDGARITPETLTRVSTYMASRGASAPASPPELMQLLRVAPQAA
                     APAASAGAPSRNFRFFDNRQKYLLFVNTCSEKEVIARRVGMELASIHPRPPAVRVFDA
                     GMGDGTVLTRVMREMHRRFATVPFYIVGKEVSLEDVRLSLARMADRFFEHPATVLVVT
                     NMYYSEAPWLTPKALAAATSLLWHEVGLGGSTAHEFSEQITALEPFLSKNWQARHSAT
                     SGNPVYERPVALVLYREDFRFLLHDVIPRQSRARADYDLVIASQPYRARVPAQFKAQR
                     VIAPLVRALGPGGRLLGIHSCGNDPGLEIVRKLWPEENPFQTNRHDVLRAVRGELGRE
                     SKHYNFNAYADARAVFRYDMHTLPSEVSSSIGTSTLFAAWNAAVYVAQIDDERLGKVI
                     GERKYLDATRDVLQAHGGLWFLDESYVVSRKRA"
BASE COUNT         1472 a         3606 c         3661 g         1415 t
ORIGIN      
        1 tgctcgcgcc cgagaacgaa aggatctgcg agaaggtggt ggcgccgacg atgatgaaca
       61 ggatcatgcc ggagatcgcg gcggtgccgc gcagcgcgcg caccaggttc gccaggctga
      121 gcgcgcggta cagcagggcg aggaacaagg tggcgaaggc gcccagggcc gcgcactcgg
      181 tgggggtggc ccagccggcg atcatggcgc ccaccaccac cacgaagatc gacaccagcg
      241 gcagcacgta ctgcaggaag ggctgcaggc gccgccagcc gcggaactcc tcgaaggcgg
      301 tgcgcggcgc cagggcgggc tcgagcttca cccgcaggat gatgtagccg atgaagacca
      361 ccgagaggat gaggcccggc acgatgccgc ccaccagcag cttggagatc gagatgcccg
      421 acaggctgcc gagcagcacc gtgagcgccg agggcgggat cagcatgtcc accgcgccga
      481 tggccatgat cgggccggtg gcgagcgtcg ggtggtagcc gcgcgagagc atcaccggca
      541 gcatcaggct gccgagcatc gccgtggtgg cgatcgtcga gcccgagatc gcggagaaca
      601 cggtgccggc caccaccgcg atgaccgcca gccgccccgg cacttcgcgc accaggcgct
      661 cgatgccgtc gatgaccttc accgcgaggc cggtgtggaa caggatctcg cccatcagga
      721 tgaacagcgg gatcggcgtc agcgcgaagc tggtcaccga ctgcacgctg ttgcgcgcga
      781 gctgcatcat gccggcgtcg ccgccgagga agatggcggc gcccaccagg ttgatggcga
      841 ggaaggcgaa ggcgaccggc aggccgagga acatcagggc ggtcgagccg cccagcagca
      901 accaggccgc cgcctgccag gtcatggcgg gccggctacg ccgccgtgac cgcgtcggtg
      961 cgcggcgcgc gctcgcccag gaggaggcgg cgcatgcgga acaggacctc gatcgccagc
     1021 agtgcgaaag ccgccggcag cggcgcgagc agccaccatt ccggcaccgc gagcaccttg
     1081 accaccatgc cgccgatggc gcggctggag agcaccgcct gcaaaccgta ccagccgatg
     1141 gccaggcagc aggcgagggc gatcaggtcg accgcccatt cgcaggccca ggcgaggcgc
     1201 aggggaaggg cgcgcagcag cacgtccacg cggatgtgca ggccctggcg cagcagccac
     1261 ggcgcggtca gcacggtgac caggtacagg gcgtattcgg tgacctcgtt ggcccacggc
     1321 agccccagca ggccgggcac gaggcgcacg ttgcgcagca ggacgtccac gcagatcgcc
     1381 gccatcatca ggaacagcac cgcgcaggcg gcgagcgcca gccccgcaag caagcggccg
     1441 taggcctcct cgagccgccg catcagcggc tgaacagctt ccgcatctgc ggcccgtact
     1501 gcgggctcac cttgatgagg ttggtccagg cgatgtcgta ggccttgtcg tagtactgct
     1561 tggcggtcgc cgcatcgaag gtgatcgtct ggatgccggc ctgcgcctgg cgccggatgt
     1621 catcctcgtt gaccttcttc caggatacgt tgtccgcctc gtgcgccacg aggtgctgca
     1681 tgaggaagtc cttctgccgc tgcgacagcg ccttccactt gtccaggttc atcaccagcg
     1741 acacctcggc gctgtagaac ccgggatcga cgcggaacct cgtcttctcg tgccagttga
     1801 agtcgaagat gccgttgatc ggccagccgt agccgtccac cgccccgcgc tcgagcgcgg
     1861 tgtacacctc gccgggcgcc gtctgcatca cggtcgcgcc gagcgcctgg aagaagtcgc
     1921 ggtacaccgg cgtgatgcgg atcttcaggc ccgtgaggtc cggcttgtcg accttcttgg
     1981 tgaggtacag gtggaacggc gtgtaatcga tgacgcggca caggtactgc atgttggcct
     2041 tctcgttcca caccttgttg atcaggtccg tcgcgccgtt gcggcgcagt tccggccccg
     2101 ccatctgcgt gagcttgagc gcgtcggcct cgggcatgat gttggtgtag aacgcgccgg
     2161 tggtcattcc gatgtccacc acgcccgtgc gcacggcgtt gccgacctca aaaggcggca
     2221 tggccttcgg cccgccgatg aagttgagct gcagcacgcc cttgccctcg gcgttgagcc
     2281 tggcgatcat cggcagcagg ttcttcacgt aggtggtgtt ctcggcaaac gccgagacca
     2341 cgcgcagggt gacttcctgc gccgccgccg cgccggccgc gaggccgagc gtcaagcaca
     2401 atactgcact ggccgttctg ttcattgctt cctcccttgg ggtgcgttca ttttacgccg
     2461 aggacttcgg cggaaggtgg atcggcgtac aggagctcga ccagcgcggc gcggcgctcc
     2521 agcaccgcac gctggttgac gtaacccttg tcggtgatct cgcccgcgtc gatcgagggc
     2581 ggctcggcga gcaccagggc gcgccgcggg tgcgtcgagc tgccgccctc cgcggcgagc
     2641 gagcgcaggg cggcggcgag ccgctcgcgc agcgcctcgg cggacaggtc cttcgccgcc
     2701 gggctgagga acagcagcgc acccacctcg ttgcggtcgt gtccggtgag caccgcgtcc
     2761 tggatcagcg gattgcccgc cgcgatcagc ttgacgcgca acgcgccgac gtgcacccag
     2821 gtgccggtgc cgagcttgaa gtcctcggcc acgcggccgt cgaacaccag gcctttctcc
     2881 ggcgccgccg ggtcggcgaa gcgcatggcg tcgccgatgc ggtagtagcc ttcctcgtcg
     2941 aaggcctcgg ccgtgaggtc cggccggcgg aggtagcccg gcgtgacgtg ggggcctttg
     3001 acgcgcactt cgagcttgcc ggcggcgggc accagcttta gctcgcagcc cgggttgggc
     3061 aggccgatga cgcccgcgcg ctcgatcggg tagtgcacgg cggcggccga gggcgcggtc
     3121 tcggtcgagc cccaggagga aagcatcgcg agttcgccct gcttctcggc gatggcgagc
     3181 gcctcgagcc gctcccacag gttctgcggc agcgcggcgc cggcgtagaa cagcatgtcc
     3241 aggttgctga agaaattgcg ccgcagcgct gcgtccttct ccaggtacgg cagcagcagg
     3301 tcgaagccgc gcggcacatt gaagtacagg gtgggcgaga cctcgcgcag gttgcgcacc
     3361 gtcgtctcca ccagcgcggg cgccggcttg ccgccgtcca cgtagagggt gccgccgttg
     3421 cgcaggacca ggttgaagca gaagttgccg ccgaaggtgt ggttccaggg cagccagtcg
     3481 acgagcgtct gcgcccgatc ctcgaggaac ggccacacct gcgccagcat ctgctggttg
     3541 gcgcacagca tgcggtgcgt gttgagcacg cctttcgggg cgccggtgga accggaggtg
     3601 aacaggatct tcgccaccgt gtccggctgc aggcgcgaga acgccagctc gagagacgag
     3661 cccggattgg tctccagcag cttcgccacc ggggtcgaga ccgcgccgac cgcggcgagc
     3721 gcgggggcga acttctccgg ctcggcggcg aacaccagcc cggggcgcag cagctcgaag
     3781 atggccttca gcttggcgaa gtccttcgac atcagggagt aggcgggcga gatcggcgcc
     3841 accggcacgc cggcgtgcat cgcgccgagc gcgagcaggg catgctcgac gccgttgtcg
     3901 gagaggatcg ccaccggccg ggaagcgtcc aggcccaggt tgagcagcgc ctgcccgatg
     3961 cgccgcgcgt ccgacagcgc gtcgcggtag ctcacccgcc gccagccgtc gcccttgcgc
     4021 tcggcgatga aggtgcgctc gggcgcgcgc tcgtaccatt gcaccagcca atcgccgacg
     4081 gcgcgcgcgt ggccgccgag cggctgcggc gagcgcagca gctgcgtgcc gtcggcgcgt
     4141 ttttccaccc ggacttcggc cggcgcgtag cgcaggcgcg tgtgcagccg catcgccatg
     4201 gcgcgaattc tagtccagca cctcgcgcag cttctccgcc ggcacgcgcg gcgccacacc
     4261 ggcgcgctcc tcctgcagca gcatcggggt gcgcgaatcg cgctcggccc agccgcgcgc
     4321 gagcgcctgc tcgaactccc gcatcgccac ctgcgccacc tgcatcggca cgccgacttc
     4381 ctgcgccagc tccagcgcga gcttcaggtc cttgtgcgcg aggcgcagcg cgaacccggg
     4441 cgggtcgtac ttgccgggca ggaactgctc ggcgaggcgg tcgaaggtgc gcttcctgcc
     4501 ggtggcgccc tggcggatcg ccctgaagag ggccagcggc tcgacgcccg ctttcacgcc
     4561 gagcgtgaag atctcggcga gcgcggtttg cacggtgaag ctcgccgtgt tgtgggcgag
     4621 cttcgccacc gagcccgcgc cgatcggccc gacatagatc ggctggtcgc cgatgacgcg
     4681 cagcacgggc tcgtggcgcc gatagacacc ctcgtcgccg cccacccaga gcgccagctt
     4741 gccgctgtgc gcgccctccc ggccgccgct cacgggcgca tcgaggaact gcacaccctt
     4801 cggctgcagc gccgcgtgca cgcggcgcac gcaggagggg gaattggtgg tgaggtcgaa
     4861 ccaggcggca tggcgcgcga gcgcggtgcc gatcaggccg gccaccgcct cgacctcggc
     4921 cgggccgggc agcgaggtga acgccagttc cgcgtccgcg accgccgccg ccggggagtc
     4981 cgcccagact gcacccgcga catcgacctt tcgcaggtcg ttcactttca cggcatggcc
     5041 ggcgcgcgcg agccgcgcgg cgatcggcgc gcccatcacg cccagtccga tgaagccgac
     5101 tctcatccgc cgcgctcctc gtagacctgc ttggccacct gcgccgcgcc gatgccgttg
     5161 gcccagccgg cgtagaaggc gagatgggtg aagagctcgg agagctcttc ctgcgtcacg
     5221 ccgttgtcga gcgcgcgcca gatgtggccc ttcagctggt cgggccggtg cagggcgatc
     5281 agcgccgcca cggtggcgag gctgcggtcg cgcctggaca acccggggcg tgcccatacg
     5341 tcgccaaaca gcaccttctc ggtcagctcg gcgagcttgg ggacgatgtc ttttaccgcc
     5401 gcgatcgtgg tgggcgggga tttggtcgct gccatggcgg tctcctgagg gtggccgccc
     5461 cggagtgtag ccgccccttc gctttttcct cgccgcgaag caaaataccg gctggactgc
     5521 cgcagggccg ccatgaagct ggaatccgtc atcgccgtca accgcttcgg cctgggcgcc
     5581 gcgccaggcg aacttgcggc ggccagcgcc gaccccaggg cctggctcgc caagcaggcc
     5641 agccagcctg cacccgccac cccggagttc ggcaagctcg cgcattcgag ccgggcgctc
     5701 gaagaatacc cgcgctggat cgcgagcctg cgcacggccg gaacgcggtc cgtggagcag
     5761 accttccgca cgcacttcgg gccgatcatg ctcgaggaac tcggcgcgcg gctcgcggcg
     5821 gcggcggcca ccgcgacgcc gttccaggag cgcctggtgt ggttctgggc gaaccatttc
     5881 acggtgtcgg cggagaagcc gctggtgttc gccctcgtcg gcggcttcga gcgcgaggcg
     5941 atccggccgc aggtgaccgg gcgcttcgcc gacctcctgc tcgccgctgc gagccacccg
     6001 gcgatgatcc tctacctgga caaccacctg tcggtgcgca agggctggca gccgcgccgg
     6061 ccctcgcgca acgcggcgtt tccctcgccc accggactca acgaaaacct cgcgcgcgag
     6121 atcctcgagc tgcacacgct gggggtgaac ggcggctaca cgcaggccga cgtcaccgag
     6181 ttcgcgcgcg tgatcaccgg ctggaccgtg cgcgcgcagg gcgagcgggg attccagttc
     6241 gacccggcgc ggcacgagcc ggggccgaag accctgctcg gcaagaccta ccccgaggag
     6301 ggcatggcgc agggcgaggc ggtgctgcgc gacctcgcgg cgcatccggc caccgcgctg
     6361 cacgtcgcca ccaagctggc gcgccatttc atcgccgacg atccgccgcc cgcggcggtg
     6421 gcgcgcatcg cgcgcgcgtt ccgcgacacc ggcggccact tgccgagcgt gtacgcggcg
     6481 ctgctcgact gcccggaggc ctgggagcgg ccgctcgcca agctgaagac cccggtcgag
     6541 tacgtcgtgt cctgccagcg cgcgctgccg gccctgcgcg cggccaggcc gcaggcgatg
     6601 ttctttacct tgcgcgggat ggggcagcgg ccgttcttcg ctccctcgcc ccagggctgg
     6661 cccgacaaca ccggggcctg ggccggcgcg gacgcgctgt ggaagcgcat cgaatgggcc
     6721 ggtatcctcg cggcgcgcct cgggacccag gtggacccgc tcgcgctcgc ccgcgagagc
     6781 tacggcgagg cggtgggcga ggcgacgagc ctcgcgatcg cacgcgccga aagccgccag
     6841 cagggcctcg cgctgtggct ggcgagcccg gaattccagc ggaggtgagc atggcgacgc
     6901 gtcgcgcagc gctcaaattc atcggcggca tggccgcctg cgccgggctg cccggcctct
     6961 cgctcggcgc cccctcggcc ggcgagcggc gcctggtgtt cgtgttcctg cgcggcggca
     7021 tggacgggct ctcggccgtc cccgcccacg gcgatccgca gtacgcggcg cggcgcggcg
     7081 cgctcgccgt ggaggcgccg ctgcgcctcg atgcgacctt cggcctcagt ccctacctgg
     7141 cggagatgca caagctctac ggcgcgggcg agctcgccgt gctgcacgcg gtggcttcgc
     7201 cgtatcgcga gcgctcgcac ttcgacgcgc agaacctgct ggagaacggc acggcgaggc
     7261 ccttcggccg cgacaccggc tggctgaacg cggcgctcgg cgccggcgcg cggccgcgcg
     7321 gccttgcgct cgggcagagc atcccgctgg tgctgcgcgg accggcgcag gtcagctcct
     7381 ggtcgccttc ggcgctgccg gtgcccgatg ccgacctgct cgagcggctc gctgcgctgt
     7441 accgcggcga tccgctcctc gagcgttcct tcgcggcggc gcgcgaggcg caggggatga
     7501 tggagcgccg cgacgcgggc ggcggggtgc aggcggtgct cgcgctcgcc aaggcggcgg
     7561 gggaaatcct cggcaagccc gaaggcccgc gcgtggcgag catcgatttc ggcggctggg
     7621 acacccacat caaccagctg ggcgaataca gcgcgctgac gcgcaacctc cgcctgctcg
     7681 accgctcggt cgcggccctg aaggcatcgc tcggcgaggc gtggcggcac accgccgtgc
     7741 tgatcgtcac cgagttcggc cgcacggtgg cgcccaacgg ctcgaacggc acggaccacg
     7801 gcaccgcggg cgcggccttc gtcgccggcg gcgcggtgcg cggcgcccgc gtgatcgcgg
     7861 actggcccgg cctcgctgag cgcgcgctgc acgagggacg cgacctgcgc ccgacgctcg
     7921 acctgcgcgc gctgttcaag gccgcgctcc ttgcgcagct ctcgctggac gaagcggtgc
     7981 tcgaggcgca ggtgttcccg gacagccggg gcgtgaagcc gctcgaggga ctatttgcct
     8041 gaggcgatgg cgcggttcat ccacgccgcg gtggcggtga cgccgccgaa actgaacagg
     8101 tgcacgccga ccacgttgca cgcggcatgc gccgcgcagt agcgggcgag ggccgccagc
     8161 tgctcgccgg gatcggtgcg cgtcaccagg cgcaccgcgt tcaatccctg cgcctgcagc
     8221 gcgcgcagcg acgcgctcac gccgcagcgc tgcgcgtagc gcagcaaggc ggcggctgag
     8281 gccgggccgg cgagccccac gtacaccggc acgccgggcg cgccgcgcgc cagcgcggcg
     8341 cagtactcca cgatccgccc cggggcgaag cagaactgcg tcaccacgta ggcggacagg
     8401 ccgtgcccgg cggcgagcgc cagcttctcg ccgaaggcct gctgcagcgc cagctgcggg
     8461 atgcgcgggt gcccctccgg atagcccggc agtccgatct cgcgcaggcc gcagtcttcc
     8521 agcaggccgc tgcggatcag cgccgcgccg tcggcgtacg gcccgcgcgc ctcgggctcg
     8581 tcgccgccca ggatcagcgc cttgcgcacg agcgcggcgc gcaggaacgc cgccagctcc
     8641 ccgcgcccgg cgatgcgccg cgcggcgatg tggggcaccg gctccagccc cgcggcgcgc
     8701 aacgcggcga tggcctccag cgaatcctcg agccggtgcc gcggcaggtg gttgacgtac
     8761 accggcgtgc ccgcgggcag gaccgcggcg atcgccgcgg cgtcctgcgg gcggtgcgcg
     8821 cccatctcga gcgagccggc cgcgaccagg tcgacggcgc ccgccgccgc gagctcaagc
     8881 gcgcttgcgg gagacgacat aggactcgtc caggaaccaa aggccgccgt gcgcctgcag
     8941 cacatcgcgc gtggcgtcga ggtacttcct ttcgccgatc accttgccga ggcgctcgtc
     9001 gtcgatctgc gccacgtaca ccgcggcgtt ccaggccgcg aacagcgtcg aggtgccgat
     9061 gctcgaggac acctcgctgg gcagggtatg catgtcgtag cggaacacgg cgcgcgcatc
     9121 cgcgtaggcg ttgaagttgt aatgcttcga ctcccggccg agctcgccgc gcaccgcgcg
     9181 cagcacgtcg tggcggttgg tctggaacgg gttctcctcg ggccacagct tgcgcacgat
     9241 ctccaggccc gggtcgttgc cgcaggaatg gatgccgagc aggcgcccgc ccggcccgag
     9301 ggcgcgcacc agcggtgcga tcacgcgctg cgccttgaat tgcgcgggca cgcgcgcgcg
     9361 gtagggctgc gaggcgatca ccaggtcgta gtcggcgcgc gcgcggctct gccgcggaat
     9421 gacgtcatgc agcaggaagc ggaaatcctc ccggtacagc accagcgcca ccgggcgctc
     9481 gtacaccgga ttgccggagg tggcgctgtg gcgcgcctgc cagtttttcg acaggaacgg
     9541 ctccagcgcc gtgatctgct cgctgaactc gtgcgcggtg ctgccgccga ggcccacttc
     9601 gtgccacagg agagaggtcg ccgcggcgag tgccttgggc gtgagccagg gcgcttcgct
     9661 gtaatacatg ttggtgacga ccagcaccgt ggccgggtgc tcgaagaagc ggtccgccat
     9721 gcgcgcgagc gacaggcgca cgtcctccag gctcacctcc ttgccgacga tgtagaacgg
     9781 cacggtggcg aagcgccggt gcatctcgcg catcacgcgc gtgagcaccg tgccgtctcc
     9841 catgccggcg tcgaacacgc gcactgccgg cgggcgcggg tggatgctcg ccagttccat
     9901 gcccacccgg cgcgcgatca cctccttctc gctgcaggtg ttgacgaaca gcaggtactt
     9961 ctgccggttg tcgaagaagc ggaagttgcg cgagggagcg cctgcggagg cggccggcgc
    10021 cgcggcctgc ggggcgacgc gcagcagttg catcagctct ggcggcgatg cgggggcgct
    10081 cgcgccgcgg ctcgccatgt aggtgctgac gcgcgtcagc gtctccgggg tgatgcgcgc
    10141 gccgtcgcgc agcc
//
LOCUS       DASRIE010000140         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3808177, whole genome shotgun sequence.
ACCESSION   DASRIE010000140 DASRIE010000000
VERSION     DASRIE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808177"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFB53_05900"
     CDS             complement(<1..496)
                     /locus_tag="VFB53_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZN86304.1"
                     /translation="MSLSNPLPWFAICALCLLIGWALARTSPFYSAELDAPGASRFDC
                     VDGLRGFLALGVFAVHAVNMYTLHTTGQWSTDIAPFHGKAATAGVALFFMITGFLFWL
                     RVLRTGPAFDTRAFFLSRLRRLTPMYAMSVLMVLVVVAAATGFTLQASPAAFAKELRP
                     WLAFG"
     gene            complement(546..1673)
                     /locus_tag="VFB53_05905"
     CDS             complement(546..1673)
                     /locus_tag="VFB53_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86305.1"
                     /translation="MKRPAVLLLGPDRDTIGGVQTHLNVLLDSELARRFGIVQFQVGS
                     AGRGENALGMLARLLISPLLLAAAIVRTGADIVHVNTSFNHRAYWRDLLYVLAARLCG
                     ARVVYQIHGGGVRMLQGSGRLRAPLMRQLLRRTLRWPDVVVVLSRRELRDASGLAPQQ
                     NVLLLPNAIDPHALLAQRRLASDPAAPLRLVYIGRLVRDKGLFELVEGLAGACARGVP
                     VQLAVAGEGPDEPRLRQAVHDLGLEEHVSFAGPAFGERKAQLLAQADVFALPSYHEGL
                     PYALLEGMAAGLVPLVTRVGAMPDVVVEGVHGRFVSMRDGESIAQAIAALAGDRARLA
                     HMSAACRERVASAYSIERLADDFTALYTSLGGTAPDLSRSA"
     gene            complement(1670..>2040)
                     /locus_tag="VFB53_05910"
     CDS             complement(1670..>2040)
                     /locus_tag="VFB53_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86306.1"
                     /translation="GASRGFDDELHLFHAERLFGEWQAHPRNPVKSDVRCARPAGNLY
                     WRDGKLYRPAQICVPRYGAGLSINRVLRLTPHAYAERQVERVLPDAAQGLLGLHTVNR
                     AGDLTVADAFTRRRRIGGAP"
BASE COUNT          319 a          718 c          762 g          241 t
ORIGIN      
        1 agccgaatgc cagccacggc cgcagctcct tggcgaacgc cgccgggctc gcctgcagcg
       61 tgaagccggt ggccgcggcg accaccacca gcaccatcag caccgacatc gcgtacatcg
      121 gcgtgaggcg ccgcaggcgc gacaggaaga aggcgcgcgt gtcgaacgcc ggaccggtgc
      181 gcagcacgcg cagccagaac aggaagccgg tgatcatgaa gaacagcgcc accccggcgg
      241 tggccgcctt gccgtggaac ggggcgatgt cggtcgacca ctgcccggtg gtgtgcagcg
      301 tgtacatgtt gaccgcgtgc accgcgaaca caccgagcgc gaggaagccg cgcaggccgt
      361 cgacgcagtc gaagcggctg gcgccgggcg catcgagctc ggcgctgtag aaggggctgg
      421 tgcgcgccag cgcccagccg atcagcaggc acagggcgca gatcgcgaac cacggcagcg
      481 ggttggagag gctcatggct accagggatt atgggaagcg ggcgtgatgc cgctgtgacc
      541 gccggctagg cggaacggct caagtccggg gccgtgccgc cgaggctggt atagagcgcg
      601 gtgaagtcgt cggcgagacg ctcgatcgag taggcgctcg ccacccgctc gcggcaggcg
      661 gcgctcatgt gcgcgaggcg cgcccggtcg ccggccagcg cggcgatggc ctgcgcgatc
      721 gattcgccgt cgcgcatcga aacgaagcgg ccatgcaccc cttcgaccac cacgtcgggc
      781 atcgcgccga cgcgggtcac gagcggcacc agcccggcgg ccatgccttc gagcagcgcg
      841 tacggcaggc cttcgtggta gctcggcagc gcgaacacgt cggcctgggc caggagctgc
      901 gccttgcgct cgccgaacgc ggggccggcg aacgacacgt gctcctccag gccgagatcg
      961 tgcaccgcct gccgcagccg cggctcgtcg ggtccctcgc ccgcgaccgc gagctgcacc
     1021 ggcacgccgc gcgcgcacgc gcccgcgagc ccctcgacca gctcgaacag tcccttgtcg
     1081 cgcaccaggc ggccgatata gaccagcctg agcggcgcag ccggatccga ggccaggcga
     1141 cgctgcgcga gcagcgcgtg gggatcgatg gcattgggca gcagcagcac gttctgctgc
     1201 ggcgcgagcc cgctcgcgtc ccgcagctcg cggcgcgaaa gcaccacgac cacgtccggc
     1261 cagcgcagcg tcctgcgcag gagctggcgc atcaggggcg cgcgcaggcg gccgctgccc
     1321 tgcagcatgc gcacgccgcc gccgtggatc tggtagacga cgcgcgcgcc gcacagcctc
     1381 gccgccagca cgtacagcag atcgcgccag taggcgcggt ggttgaacga ggtgttgacg
     1441 tgcacgatgt cggcgccggt gcgcacgatg gccgcggcca gcagcaaggg gctgatcagc
     1501 aggcgcgcca gcatgccgag cgcgttttcc ccgcgcccgg cgctgccgac ctggaactgc
     1561 acgatgccga agcggcgcgc aagctcggaa tcgagcagca cgttgaggtg ggtctgcacc
     1621 ccgccgatgg tgtcgcggtc cgggccgagc agcagtaccg ccgggcgctt catggcgcgc
     1681 cgccgatccg gcggcgccgg gtgaaggcgt cggcgaccgt gaggtcgccg gcgcggttga
     1741 cggtgtgcag cccgagcagg ccctgcgcgg catcgggcag cacgcgctcc acctggcgct
     1801 cggcgtaggc atgcggggtg agccgcagca cgcggttgat cgagagtccc gcgccgtagc
     1861 gcggcacgca gatctgcgcc ggccggtaga gcttgccgtc gcgccagtag aggttgcccg
     1921 ccgggcgcgc gcagcgcacg tcggacttga ccggattgcg cgggtgcgct tgccattcac
     1981 cgaacaggcg ctcggcgtgg aacaggtgca gctcgtcgtc gaagccgcgg ctcgcgccgg
//
LOCUS       DASRIE010000141        15206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3809331, whole genome shotgun sequence.
ACCESSION   DASRIE010000141 DASRIE010000000
VERSION     DASRIE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809331"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFB53_05915"
     CDS             complement(<1..1369)
                     /locus_tag="VFB53_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZN86307.1"
                     /translation="MNSPKRGRARGTLKGRGVDPAAREEVRALLGGDPCRRDLLIEYL
                     HRIQDRYGHLSAARLAALAEEMRLSQTEVYEVATFYHHFDVVKEGETPPPAVTVRVCD
                     SLSCEMAGAGALLDRLPGVLGREVRVLHAPCVGRCESAPVAVVGQNPILNASLSAVEK
                     AVAEKLVRCPETPYINFNAYRKAGGYRLIADCLAGKRSRDEITKIMEDSALRGLGGAG
                     FPTGRKWRIVAAEPEPRVMAVNIDEGEPGTIKDRWYLERDPHRFLEGMLVAAWCAGVG
                     EIYIYLRDEYAGCRKILEDEIRKLKAAAPCALPPIHLRRGAGAYICGEESAMIESIEG
                     KRGMPRLRPPYVAQVGVFGRPTLEHNMETLFWVRDFVERGTGWFTKEGRHGRKGLRSF
                     SVSGRVKRPGVHVAPAGITLLELIDEYCGGMLDGHTLYGYLPGGASGGILPASLANVP
                     LDFHTL"
     gene            1472..2050
                     /locus_tag="VFB53_05920"
     CDS             1472..2050
                     /locus_tag="VFB53_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86308.1"
                     /translation="MASPVDPNDVMMTGENSFIRYSPDGGKTQTDRMSHWRVLWCPAG
                     AGHALFARSTLTGGAVRIWSDNPPVARWLQKEIESLLFPAFADAATPVTAAAFARLGG
                     IDTRAAELVTAKDTEVLLSWEDFLAPFVLNMPPGSGGRPIGVFSTFFPAKAARIALNG
                     RVAAGAPYAEKRGERDSTSACLAWSETWVRPR"
     gene            2060..3034
                     /locus_tag="VFB53_05925"
     CDS             2060..3034
                     /locus_tag="VFB53_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN86309.1"
                     /translation="MKFKPLGRTGVQVSELCFGTMSFGGDADEATAAQMYKACRDAGI
                     NFFDCADQYSKGKAEEILGRLVKGHRDQLVLTTKCYNPSSEDINDRGTSRRHVARAVE
                     ASLKRLQTDRVEVLFLHQYDARTPIEESMRALEDLVRSGKVLYPAVSNWSAWQTQRAV
                     DIQERNSWARLQVHQPMYNLVKRQAEVEILPMAEANGIGVIPYSPGAAGLLSGKYSGE
                     ASGRLKTNKMYEARYGEPWMFEVAETFVDFCRQKGLHPVSAAVAWVGAHPAITAPIIG
                     ARNLDQLKDSLNAAKVDMSAALRAEITALSRTPPPATDRLEEIRPPKA"
     gene            complement(3031..3765)
                     /locus_tag="VFB53_05930"
     CDS             complement(3031..3765)
                     /locus_tag="VFB53_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN86310.1"
                     /translation="MPTPVSFDPDVGRQILKYARLLSARGYVHNTLGNVVVRVPHPDF
                     PHGVAYTKHAELSLEEMELDNIVVTDIPTSRVLHGSKVTSIGHNLSREILRLRPDIHA
                     TIHVHDDALIAFFGSGAYDGVGMLSLDPPFVLGKPVHLVPAHVDVEADVSGVKDFIQG
                     TNTVVLVGHGVTALGRTLSEAYHRLNTLTAEVRRNIVAEVLAHLKGSRPSYRGQAEIE
                     SMYRFAESIIYPKRATRVMREDEAAG"
     gene            3797..4720
                     /locus_tag="VFB53_05935"
     CDS             3797..4720
                     /locus_tag="VFB53_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZN86311.1"
                     /translation="MCRALLYLGQPVLLDDLLFQPDSALVKQSTMPKMLHLLNLAGFG
                     MRAWDGASHEPERPFAYASDSIPVFDRNLKGLAEKIRARCLLAHVRGVPYNTRVEIAT
                     QNVHPFQFAGVPLVMAHNGDLARFAQMKPLLAPQVKPEFAAQIRGTTDSEWMYALVVS
                     RLKDPHARPSGEELHDAIAHMLGVVRAARAELGIALASPVNLFLTDGTQLAAVRYTFD
                     FGCYPPDPAEFLPGHLNYYSLWVTLGRDYGLHDGEWKMTGGSENAEAVLVASEPLTRD
                     VSAWVEVPEYSMLHAALRRNGQPALTLRDLA"
     gene            complement(4721..5740)
                     /locus_tag="VFB53_05940"
     CDS             complement(4721..5740)
                     /locus_tag="VFB53_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN86312.1"
                     /translation="MSADALQQSGLAPLLAVAQPVSFTKGARLLQQGAASRGAYVVRE
                     GTAEAIVTLPGGEKLTVAKLGPGSVFGEMALLERGVCMATVVATANLDGWFLDRDDFR
                     VLAAQRSPEARRLRHALTLILAGKLSAMNAKVLEAAAPDEPAAARVQGADPLAGVKRV
                     KKSGFDVRPFLPLLPAFDGFDEAEIAEVLDAARLLELPAGHGVFGIGQPSSAAFVVLR
                     GAVEIHAAHAGRERRMAVLGPGQIFGFMSLLAGGAHGSAARVRESAVLLEIPKAAFEA
                     LYHGDGTVCAALHHAVQRSLLGSLAQTNRHLTRLISLARLRGAKKESGKLETALGGQI
                     VAATD"
     gene            5770..8595
                     /locus_tag="VFB53_05945"
     CDS             5770..8595
                     /locus_tag="VFB53_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN86313.1"
                     /translation="MNQAAADIAPERVLAVVQAVAREARPHLEPHVALDSSLEKELGL
                     DSLARVELVLRLEREFGASLPERALATSETPRDLLRFLLAGAGQAPQAADRSVAGLAQ
                     GEGVRAPSEAQARTLTEALAYHVERQPDRLTVFMYEGENEFPLSYRALWDGALGYAAA
                     LARAGLQRGQMVAIMLPTCKEYLYSFYGVLLAGGVPVPLYPPARLTTIEDHMTRHVSI
                     LQSAGATIMITVPEAKALAYLLRAQVETLRQVLVPADLAGAVERSFQPVHGNPGDMGF
                     LQYTSGSTGTPKGVVLSHANLMANVRAMGAAVNTSAADVFVSWLPLYHDLGLIGANFA
                     ALVLGFPTVLMSPLAFLSRPVSWMRALHRHRGTLSGGPNFSFELCLRRIADEDMEGID
                     LSSWRFAFNAAEPVSPETIVAFEKRFARWGLRGNCLSPSYGLAESSVGVAITVPGTPW
                     IADRLDREQFRRTGEAREARADDASPLVVIGCGTPIPGHDIRVVDAAGLELPDRQEGL
                     LQFRGPSSTSGYYRNPAATKSLFAGEWVNTGDRAYLSQGTLFLTGREKDIIIRGGRNI
                     SPYELEQAVGDLAGVRRGCVAVFGSRDAASGTERVVVLAEMRDADASRHESLKRMINE
                     LSVSLLGGPADDIVLAPPGTVPKTSSGKIRRVAAREYYDRGPSSVKPQAVWLQFLRLV
                     LAGAAPQLRRGWRSVLGGLYALRVYNAFALLFPFALLPAAVAGPTLTWRIGSAVCRLF
                     LRAAGIPVVLRGAEQLPRGPAVLAVNHTSYLDAVVLVALLGPRVHAFVAKSEFQENPL
                     MRALLGGFGTLFVERFDVQKSAEHAGTLAGAVRAGASLVVFPEGTLTRHTGLMEFRAG
                     AFLAAAQSGVPVVPVALRGVRSVLRDGTWYARRAAIAVTICAPIAPDGTDWAAALRLR
                     DRVRAEILKFCGEPDLLPPAKGAAPSA"
     gene            complement(8585..11041)
                     /locus_tag="VFB53_05950"
     CDS             complement(8585..11041)
                     /locus_tag="VFB53_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86314.1"
                     /translation="MLGRLFGWKPRPAPASGAAEALAQAHALHQAGKLEEARAGYASV
                     LAREPRNFTALQLLGVLALQAARPAEAVEHFSLALQVDPASAAAHNNLGEARRRTGRL
                     EEAEACFREAVSRDPAYADAHFNLAALLRRLGRREEAAASYRSAIAARPAFAEAQLRL
                     GETLNALGRPDQAIEALRAALALDADMAEAHSSLGVALSDAGRLDEALVECQRAVALK
                     PGLAVAHFNAGNVLREKGFLEEAIAAYRAAIERDAQIVEAYNNLGNLLKHQGKAAEAM
                     QCYEQAVALSPAFAEARLNAAKLLRESGRHGDAVAAYRALLEGRPDLAEAHFDLGNAL
                     KAMGDKSGALACYRKALEIDPGHAEARWAYAMSQLPLVAADEAEPVRSRAEFAAELEA
                     LARWCEGRGAEKTARAVGTQQPFYLAYQEDDHRDLLARYGALCARLMGVWQEAAGLSR
                     VAGVTRAEIRVGIVSAHIRDHSVWNAIIKGWLQRLDRGRFDVRLFHLGQANDVETALA
                     KTMATHYTYGKSDLVEWSQLILGHQLDVLVYPEIGMDPVTAKLASLRLAPLQAAAWGH
                     PHTSGLPTIDCFLSAEALEPAGAEAHYTERLVRLPGLGCCLEPAKIVPAAVDLAALGI
                     REGVPLLICAGVPFKYAPRHDAVLAQIARRLGDCQFVFFTPTGADALMHRLRERLARS
                     FAAAGLELESCAVFVPWQNRAQFYGLLAQADVFLDTIGFSGFNTALQAIEAGLPIAAW
                     EGRFLRGRLASGLLRRMGLHELVADTPQGYADIAVRLAQDAAWWGEMRERMVASRARL
                     YGDAAPVRALEQFFETAVRR"
     gene            complement(11050..11448)
                     /locus_tag="VFB53_05955"
     CDS             complement(11050..11448)
                     /locus_tag="VFB53_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86315.1"
                     /translation="MARLVRRAHHVEWRLHHASEALMLGDWFNTKEVDAVADEIVADL
                     VKRLPPSPGPHTRKTADRLRKTHDVIFARLDQFARSRKLNIYKKARLGNRVRWALREA
                     GYAPEFTAALSYELVTVATLAARQPGKTGG"
     gene            complement(11415..13292)
                     /locus_tag="VFB53_05960"
     CDS             complement(11415..13292)
                     /locus_tag="VFB53_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86316.1"
                     /translation="MLRRRLADALIASGGRAERGGRLAGACRRYRMAAALAPGYPPAL
                     LNLGAALEAAGDTSAATRAYEALLAVRAADPYANYNLGRLRHTAGAQEEAERLLRAAL
                     LAKPDFADARVVLANVQDARGDLAAATASLEAALALRPASGGAWYNYGELLWKQSRYD
                     EAEAALRRALEIEPELVPAWYLLGNMLRGTSRLDEALAAFAAARRLEPLRFDLESMEL
                     LVLTLRDALSAEEVYERHRAFGARLEAAVPAHAGPYAGSREPGRRLRIGYVSSDFYRH
                     PVAWFLLPLLERHDRAGFEAILYSTGDKVDEVTGQVKAAADGWRDARAMSDEALAQAI
                     ERDAIDVLVDLTGHAGALRLGVFARRPAPVQFTWLGYLHSTGLTRIDYRLTDARADPP
                     GASERLHTESLLRLPHCLWCYRPLHAAAHAAEAPWRRNGFVTFGSFNHAPKLTASSRR
                     IWAEILRQLPQARLRLVGVPDGRARADLLRDFAAAGVPAARLTILPRVSFGEYLEQFD
                     AVDIALDSMPYGGGTTTFDTLWMGVPVLTLAGDRPASRSAASILGALGLQDWVAATPA
                     EYLRLALAHAGATGRLEELRGTLRQRLRASPLMDEARFARDVEAAYRQAWRAWCGARI
                     T"
     gene            complement(13293..14177)
                     /gene="sucD"
                     /locus_tag="VFB53_05965"
     CDS             complement(13293..14177)
                     /gene="sucD"
                     /locus_tag="VFB53_05965"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZN86317.1"
                     /translation="MSILIDKHTRVVTQGITGKTGMFHTQTSRAYAGGKDAFVAGVNP
                     KKAGENFEGIPIFGTVKEAKEEAGVNTSVIYVPPPFAAAAIWEAVEADLDLVICITEG
                     IPVRDMIEVRDRMRKQKRRTLLVGPNCPGVITPGEIKIGIMPGHIHKPGRVGVVSRSG
                     TLTYEAVGQLTELGIGQSSAVGIGGDPVNGMKHVDVMRLFNDDPGTDAVIMIGEIGGS
                     DEEDAARWVKANMRKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAQEKLAVMESCGI
                     KVTRNPAEMGRLLKSVLK"
     gene            complement(14249..15206)
                     /gene="sucC"
                     /locus_tag="VFB53_05970"
                     /pseudo
     CDS             complement(14249..15206)
                     /gene="sucC"
                     /locus_tag="VFB53_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
BASE COUNT         2141 a         5490 c         5289 g         2286 t
ORIGIN      
        1 tgagagtgtg aaagtcgagc gggacgtttg ccagcgacgc cggcaggatg ccaccagacg
       61 cgccacccgg caggtacccg tacagggtgt gaccgtccaa catccctccg cagtactcgt
      121 cgatgagctc cagcaatgtg atgcctgctg gtgcgacgtg aacccccggt cgcttgacgc
      181 gcccgctgac ggaaaacgag cgcagcccct tgcgcccgtg gcggccttcc ttcgtgaacc
      241 acccggtgcc gcgctcgacg aagtcgcgca cccagaaaag cgtctccatg ttgtgctcga
      301 gggtgggccg gccgaacaca cccacctgcg cgacgtacgg cgggcgcagg cgcggcatgc
      361 cgcgcttgcc ctcgatcgac tcgatcatcg ccgactcctc gccgcagatg taggcgccgg
      421 cgccgcggcg caggtggatc ggcggcaggg cgcagggcgc agccgccttc agcttgcgga
      481 tctcgtcttc gaggatcttc ctgcagccgg cgtactcgtc gcgcaggtag atgtagatct
      541 cgcccacccc ggcgcaccaa gcggccacca gcatgccttc gaggaaccgg tgcggatcgc
      601 gctcgaggta ccagcggtcc ttgatggtgc ccggctcgcc ttcgtcgatg ttcaccgcca
      661 tcacgcgcgg ctcgggctcg gcggcgacga tgcgccactt gcggccggtc gggaagcccg
      721 cgccgcccag gccgcgcagc gccgagtcct ccatgatctt cgtgatctcg tcgcggctgc
      781 gcttgcccgc caggcagtcc gcgatcaggc ggtagccgcc ggccttgcgg tacgcattga
      841 aattgatgta gggcgtctcg gggcagcgaa ccagtttttc tgcaacggct ttctcaacgg
      901 cagacagcga agcgttgaga atcgggttct ggccgaccac cgccaccggc gccgattcgc
      961 agcgccccac gcagggggcg tgcagcacgc gcacctccct gccgaggacg cccggcagcc
     1021 tgtccagcag ggcgcccgcg cccgccattt cgcaggacag cgaatcacag acgcgaaccg
     1081 tgactgcggg cggcggcgtt tcgccttcct tcaccacgtc gaagtggtgg tagaacgtcg
     1141 ccacctcgta gacctcggtc tgcgacagcc gcatctcctc ggcgagcgcc gcgaggcgcg
     1201 ccgccgacag gtggccgtag cggtcctgga tgcggtgcag gtattcgatc agcaggtcgc
     1261 gccggcaggg gtcgccgccc agcagcgccc tgacttcttc gcgcgccgcg gggtcgacac
     1321 cgcggccttt cagcgtgccg cgtgccctgc cgcgcttcgg ggaattcatc ggcctcattt
     1381 tacgggccgc tgcgccgggg ggcgccttga cagcggtcaa gattccgcgc gcgctgcgtt
     1441 accatcgcgc caacaaccgg aggaatcgaa catggcctcg cccgtggatc cgaacgacgt
     1501 gatgatgacc ggcgaaaact ccttcatccg ctacagcccc gacggcggca agacgcagac
     1561 cgaccgcatg agccactggc gcgtgctgtg gtgtccggcg ggtgcgggcc acgccctgtt
     1621 cgccaggagc accctcaccg gcggcgcagt gcgcatctgg tcggacaacc cgccggtggc
     1681 tcgctggctg cagaaggaga tcgagtcgct gctgtttccg gccttcgccg acgcggcgac
     1741 gccggtgacg gccgccgcgt tcgcccgcct gggcggcatc gacacccggg cggccgagct
     1801 ggtcacggcg aaggacacag aagtgctgct gtcctgggaa gacttcctcg cacccttcgt
     1861 gctcaacatg ccgccgggct ccggcgggcg gccgatcggc gtgttcagca cctttttccc
     1921 cgccaaggcc gcccgcatcg cgctgaacgg ccgcgtcgcc gcgggggcgc cctacgcgga
     1981 gaagcgcggc gagcgcgatt cgaccagcgc ctgcctggcg tggtccgaaa cctgggtccg
     2041 gccaaggtag actcgcgcga tgaaattcaa acccctcggc cgcacgggcg tacaggtttc
     2101 cgaactgtgc ttcggcacca tgtccttcgg cggggacgcc gacgaagcga ctgccgccca
     2161 gatgtacaaa gcctgccgcg acgcgggcat caacttcttc gactgcgccg accagtacag
     2221 caagggcaag gcggaggaga tcctcggccg gctggtgaag ggccaccgcg accagctggt
     2281 cctcaccacc aagtgctaca acccgtcgtc ggaggacatc aacgaccgcg gcacctcccg
     2341 ccggcacgtc gcacgcgcgg tggaggcgag cctcaagcgc ctgcagaccg accgcgtcga
     2401 ggtgctgttc ctgcaccagt acgatgcgcg caccccgatc gaggagtcga tgcgcgccct
     2461 ggaggacctg gtgcgctcgg gcaaggtgct ctatcccgcg gtgagcaact ggtccgcctg
     2521 gcagacgcag cgcgcagtgg acatccagga gcgcaacagc tgggcgcggc tgcaggtgca
     2581 ccagcccatg tacaacctgg tgaagcgcca ggccgaggtc gagatcctgc cgatggccga
     2641 ggccaacggc atcggcgtga ttccctacag ccccggcgcc gcgggcctgc tgtcgggcaa
     2701 gtacagcggg gaagcgagcg gcaggctgaa gaccaacaag atgtacgagg cgcgctacgg
     2761 cgagccctgg atgttcgagg tggcggagac gttcgtcgac ttctgcaggc agaaaggcct
     2821 gcatccggtg agcgccgcgg tggcctgggt cggggcgcac ccggcgatca ccgcgccgat
     2881 catcggcgcg cgcaatctcg accagctgaa ggactcgctc aacgcggcca aggtggatat
     2941 gagcgctgcg ctgcgcgccg agatcaccgc gctgtcgcgc acgccgccgc cggccaccga
     3001 ccggctcgaa gagatcaggc cgccgaaggc ctagcccgcc gcttcgtctt cgcgcatcac
     3061 ccgggtcgcg cgcttcggat agatgatgga ctcggcgaag cggtacatgc tctcgatctc
     3121 cgcctggccg cggtagctcg ggcgactgcc cttcaagtgc gccagcacct cggcgacgat
     3181 gttgcgccgc acctcggccg tcagcgtatt caagcggtgg taggcctcgg agagcgtgcg
     3241 accgagcgcg gtgacgccgt ggcccaccag caccacggtg ttggtgccct ggatgaagtc
     3301 cttcacgccg ctgacgtcgg cctcgacgtc cacgtgcgcc ggcaccaggt gcaccggctt
     3361 gccgagcacg aacggcggat cgagcgacag catgccgacg ccgtcgtagg cccccgagcc
     3421 gaagaacgcg atcagcgcgt cgtcgtgcac gtggatggtg gcgtggatgt ccggccgcaa
     3481 ccgcaggatc tcccggctca ggttgtggcc gatgctggtg accttgctgc cgtgcagcac
     3541 gcggctcgtc ggaatgtcgg tgacgacgat gttgtcgagt tccatctcct cgagcgacag
     3601 ctcggcgtgc ttggtgtagg ccacgccgtg gggaaaatcc gggtgcggta cgcgcaccac
     3661 gacgttgccg agggtgttgt ggacgtagcc gcgcgccgag agcagccgcg cgtacttgag
     3721 gatctggcgg ccgacgtcgg gatcgaagct gacgggcgtg ggcatggcgg ttgcggttca
     3781 gtctatcatc gcgtccatgt gccgcgccct gctctacctc ggccagccgg tgctgctgga
     3841 cgacctgctg ttccagccgg attcggcgct ggtcaagcag agcaccatgc cgaagatgct
     3901 gcacctgctc aacctcgcgg gcttcggcat gcgcgcctgg gacggcgcct cgcacgagcc
     3961 cgagcgcccg ttcgcctacg cctcggactc gatcccggtg ttcgaccgca acctgaaggg
     4021 gctggcggag aagatccgcg cgcgctgcct gctggcgcac gtgcgcggcg tgccctacaa
     4081 cacccgggtc gagatcgcca cgcagaacgt gcaccccttc cagttcgcgg gggtgcccct
     4141 ggtgatggcg cacaacggcg acctggctcg cttcgcccag atgaagccgc tgctcgcgcc
     4201 gcaggtgaag cccgagttcg cggcgcagat ccgcggcacc accgacagcg aatggatgta
     4261 cgcgctggtc gtgtcgcgcc tcaaggaccc gcacgccagg ccctccggcg aggagctgca
     4321 tgacgcgatc gcgcacatgc tcggcgtggt gcgcgcggcg cgcgccgagc tcggcatcgc
     4381 gctcgcctcg ccggtgaacc tgttcctgac cgacggcacg cagctcgccg cggtgcgcta
     4441 caccttcgac ttcggctgct atccgccgga ccccgccgag ttcctccccg gccacctcaa
     4501 ttactactcg ctgtgggtga cgctgggccg cgattacggc ctgcacgacg gcgaatggaa
     4561 gatgaccggc ggcagcgaga acgccgaggc ggtgctggtc gcctccgagc cgctgacgcg
     4621 cgacgtctcg gcctgggtgg aggtgcccga atactcgatg ctgcacgccg ccctgcggcg
     4681 caacggccag ccggccctca cgctgcgcga cctcgcctga ctagtcggtg gcggccacga
     4741 tctggccgcc gagcgccgtc tccagcttgc cgctttcctt cttcgccccg cgcaggcgcg
     4801 ccaggctgat caggcgcgtg aggtgccggt tggtctgcgc gagcgagccg agcaggctgc
     4861 gctgcaccgc gtggtgcagc gccgcgcaca ccgtgccgtc gccgtggtac agggcctcga
     4921 acgcggcctt cgggatctcc agcaggaccg ccgactcgcg cacgcgcgcg gcggaaccgt
     4981 gcgcgccgcc ggcgagcagg ctcatgaagc cgaagatctg gcccggcccg agcaccgcca
     5041 tgcgccgctc gcggccggca tgcgcggcgt ggatctccac cgcgccgcgc agcaccacga
     5101 acgcggcgct cgaaggctgg ccgatgccga acacgccgtg tccggccggc agctcgagca
     5161 gccgcgcggc gtcgagcact tcggcgatct ccgcctcgtc gaaaccgtcg aacgcgggca
     5221 acagcggcag gaagggccgg acgtcgaagc ccgatttctt cacgcgcttc acgccggcca
     5281 ggggatccgc gccctggacg cgcgccgccg caggctcgtc cggcgccgca gcctccagca
     5341 ccttggcgtt catggcgctc aacttgccgg cgaggatcag cgtcagcgcg tggcgcaggc
     5401 gccgcgcctc gggcgagcgc tgcgcggcga gcacgcggaa gtcgtcgcga tcgaggaacc
     5461 agccgtcgag gttggcggtg gccaccacgg tggccatgca cacgccgcgt tccaggagcg
     5521 ccatctcgcc gaacacgctc ccgggcccca gcttcgccac cgtgagcttc tcgccgccgg
     5581 gcagcgtgac gatcgcctcc gcggtgccct cgcgcacgac ataggcgccg cgcgaggccg
     5641 cgccctgctg cagcagccgc gcgcccttgg tgaacgacac cggctgcgcc accgcgagca
     5701 gcggcgcaag cccggattgc tgcaacgcgt cggcggacat gcagtgcgcg agtgtacact
     5761 cgccacgcca tgaaccaggc cgcggccgac atcgctccgg agagggtcct cgccgtggtg
     5821 caggccgtgg cgcgcgaggc gcgcccgcac ctggagccgc atgttgcgct cgacagctcg
     5881 ctggaaaagg agctgggact ggacagcctg gcgcgcgtcg agctggtgct gcgcctggag
     5941 cgcgaattcg gcgccagcct gccggaacgg gcgctcgcca ccagcgagac gccgcgcgac
     6001 ctgctgcgct tcctgctggc gggcgcgggc caggcgccgc aggccgcgga ccgcagcgtc
     6061 gccggcctgg cgcagggcga aggcgtgcgc gcgccctccg aggcccaggc gcgcacgctc
     6121 accgaagcgc tcgcctacca cgtcgagcgc cagcccgacc ggctgacggt gttcatgtac
     6181 gagggcgaga acgagtttcc gctcagctac cgcgcgctgt gggacggcgc gctgggctac
     6241 gcggcggcgc tggcgcgcgc gggcctgcag cgcgggcaga tggtggcgat catgctgccc
     6301 acctgcaagg agtacctgta ctccttctac ggcgtcctgc tcgccggcgg cgtgccggtg
     6361 ccgctctacc cgccggcgcg cctgaccacc atcgaagacc acatgacgcg ccacgtcagc
     6421 atcctgcaga gcgcgggcgc gacgatcatg atcacggtgc ccgaggcgaa ggcgctcgcc
     6481 tacctcctgc gcgcgcaggt ggagacgctc cgccaggtgc tggtgcccgc cgacctggcc
     6541 ggcgcggtgg agaggagttt ccagccggtg cacggcaatc ccggcgacat gggtttcctg
     6601 cagtacacct cgggcagcac cggcacgccc aagggcgtgg tcctgtcgca cgccaacctg
     6661 atggccaacg tacgtgccat gggcgcggcg gtgaacacca gcgccgcgga cgtgttcgtg
     6721 agctggctgc cgctgtacca cgacctgggg ctgatcggcg ccaacttcgc ggcgctggtg
     6781 ctcggcttcc cgacggtgct gatgtcgccg ctcgctttcc tgtcgcggcc ggtgagttgg
     6841 atgcgcgccc tccaccgcca ccgcggcacc ctgtccggcg gacccaattt ctcgttcgag
     6901 ctgtgcctgc gccgcatcgc cgacgaggac atggaaggca tcgacctgtc ctcctggcgc
     6961 ttcgccttca atgccgcgga gccggtgagt cccgagacca tcgtcgcgtt cgagaaacgc
     7021 ttcgcgcgct ggggcctgcg cgggaactgc ctgtcgccct cctacgggct ggcggaatcc
     7081 tcggtgggcg tggcgatcac cgtgccgggc acgccctgga tcgcggatcg cctggaccgc
     7141 gagcagttcc ggcgcacggg cgaggcgcgc gaggcgcgcg ccgacgatgc ctcgccgctc
     7201 gtcgtcatcg gctgcggcac gccgatcccc gggcacgaca tccgggtggt ggatgccgcg
     7261 gggctcgagc tgcccgaccg gcaggaagga ctgctgcagt tccgcggccc gtcgtcgacc
     7321 tcgggctatt accgcaatcc ggcggcgacc aagagcctgt tcgccggaga gtgggtcaac
     7381 accggcgacc gcgcctacct gtcgcaaggc acgctgttcc tcaccggccg cgagaaggac
     7441 atcatcatcc ggggcggacg caacatcagc ccctacgaac tggagcaggc ggtcggcgac
     7501 ctcgccggcg tgcggcgcgg ctgcgtggcg gtgttcggca gccgcgacgc ggccagcggc
     7561 accgagcgcg tggtggtgct ggcggaaatg cgcgacgcgg acgcgtcgcg ccacgagagc
     7621 ctcaagcgca tgatcaacga gctgtcggtg tccctgctcg gcggcccggc cgacgacatc
     7681 gtgctcgccc cgccgggcac cgtgcccaag acctccagcg gcaagatccg ccgcgtcgcg
     7741 gcgcgcgaat actacgatcg cgggccctcc tcggtgaagc cgcaggcggt gtggctgcag
     7801 ttcctgcgcc tggtgctggc cggcgccgcg ccgcagctgc gccgcggctg gcgcagcgtc
     7861 ctgggcggtc tctacgccct gcgcgtgtac aacgccttcg ccctgctgtt ccctttcgcg
     7921 ctgcttcccg ccgcggtcgc cgggccgacg ctgacctggc ggatcggcag cgcggtgtgc
     7981 cggctgttcc tgcgcgcggc gggcatcccg gtggtgctgc ggggggcgga gcagctgccg
     8041 cgcggccccg cggtgctcgc ggtgaaccac accagctacc tcgacgccgt ggtgctggtg
     8101 gcgctgctcg ggccgcgcgt gcacgcgttc gtcgccaaga gcgagttcca ggaaaacccg
     8161 ctgatgcgcg ccctgctcgg gggcttcggc acgctgttcg tcgagcgctt cgacgtgcaa
     8221 aagagcgccg agcatgccgg cacgctggcc ggggcggtgc gcgccggcgc ctcgctggtg
     8281 gtgtttcccg aaggcacgct gacgcgccac accgggttga tggagttccg cgcgggggcg
     8341 ttcctggcgg ccgcccagtc gggcgtgccg gtggtgccgg tggccctgcg cggcgtgcgc
     8401 tcggtgctgc gcgacggcac ctggtacgcg cggcgcgccg ccatcgcggt caccatctgc
     8461 gcgccgatcg cgcccgacgg caccgactgg gcggcggcat tgcgcctgcg cgaccgcgtg
     8521 cgcgccgaga tcctgaagtt ttgcggcgag ccggacctgc tgcccccggc gaaaggcgca
     8581 gcccctagcg cctgaccgcg gtctcgaaga actgctcgag ggcgcgcacc ggtgccgcgt
     8641 cgccgtacag cctggcgcgc gacgcgacca tgcgctcgcg catctcgccc caccaggcgg
     8701 catcctgcgc caggcgtacc gcgatgtccg catagccctg cggggtgtcc gccaccagtt
     8761 cgtgcaggcc catgcgccgc agcaggccgc tcgccaggcg cccgcgcagg aacctgccct
     8821 cccaggccgc gatgggcagc cccgcctcga tcgcctgcag cgccgtgttg aaaccggaga
     8881 agccgatggt gtcgaggaac acgtcggcct gcgccagcag gccgtagaac tgcgcgcggt
     8941 tctgccatgg cacgaagacg gcgcaggact ccagctcgag ccccgcagcg gcgaacgatc
     9001 gcgccaggcg ctcgcgcagc cggtgcatca atgcgtcggc cccggtcggc gtgaagaaga
     9061 cgaattggca gtctcccagc cggcgcgcaa tctgcgccag cactgcgtcg tgccgcggcg
     9121 cgtacttgaa cgggacgccc gcgcaaatca gcagcggaac gccctcgcgg atgcccagcg
     9181 cggcgagatc cactgccgcg gggacgatct ttgccggctc caggcagcag cccaggcccg
     9241 gcaggcgcac cagcctctcg gtgtagtgcg cctcggcgcc cgccggctcg agcgcctcgg
     9301 ccgaaaggaa gcagtcgatg gtcggcagcc cgctggtgtg cggatggccc caggccgccg
     9361 cctgcagggg cgccaggcgc aggctggcga gcttcgccgt gaccgggtcc atgccgattt
     9421 ccgggtatac cagcacgtcg agctggtggc cgagaatcag ctgcgaccat tccaccaggt
     9481 cggacttgcc gtaggtgtag tgcgtcgcca tggtcttggc gagcgcggtc tcgacgtcgt
     9541 tcgcctgccc gaggtggaac aggcgcacat cgaaacggcc gcggtccagc cgctgcagcc
     9601 agcccttgat gatcgcgttc cataccgaat ggtcgcggat gtgcgccgac acgatgccga
     9661 cgcggatctc cgcgcgcgtg acgcccgcca ccctgctcag gcccgccgcc tcctgccaga
     9721 cgcccatcag gcgcgcgcac agcgcgccgt aacgcgccag cagatcgcgg tggtcgtcct
     9781 cctggtaggc gaggtagaac ggctgctgcg tgcccacggc gcgcgccgtc ttctcggcgc
     9841 cccggccctc gcaccagcgc gcgagcgcct cgagttccgc ggcgaactcg gcccggctgc
     9901 gcaccggctc cgcttcgtcg gcggccacca gcggcagctg ggacatggcg taggcccagc
     9961 gcgcctcggc gtggcccgga tcgatttcca gcgccttgcg gtagcaggcg agcgcgccgg
    10021 acttgtcgcc catcgccttc agcgcattgc ccaggtcgaa atgcgcctcc gccagatccg
    10081 ggcgcccctc gagcagcgcg cggtaggccg cgacggcatc gccgtgccga ccgctctcgc
    10141 gcagcagttt cgcggcgttg aggcgggctt ccgcgaacgc gggcgacagc gcgactgcct
    10201 gctcgtagca ttgcatcgcc tcggccgcct tgccctggtg cttgaggaga ttgccgagat
    10261 tgttgtaggc ctcgacgatt tgcgcatcgc gctcgatggc ggcgcggtag gccgcgatcg
    10321 cctcttcgag gaagcccttt tcgcgcagca cgttgcccgc attgaaatgc gcgacggcga
    10381 gcccgggctt gagggcgacc gcgcgctggc actcgaccag cgcctcgtcg agcctgccgg
    10441 cgtcgctcag ggcgacgccg aggctgctgt gcgcctccgc catgtccgcg tccagggcga
    10501 gcgccgcgcg cagcgcctcg atcgcctggt ccgggcgccc gagcgcattc agcgtttcgc
    10561 ccaggcgcag ctgcgcttcc gcgaacgccg gcctggcggc gatggcgctg cgataggacg
    10621 ccgcggcctc ctcgcggcgc ccaaggcgcc gcaacagtgc ggccaggttg aagtgcgcgt
    10681 cggcatatgc gggatcccgc gagacggctt cgcggaagca ggcttcggct tcctccagcc
    10741 gcccggtccg gcgcctggcc tcgccgagat tgttgtgcgc ggccgcgctg gcgggatcga
    10801 cctgcagcgc cagggagaag tgctcgacgg cctccgccgg ccgggcggcc tgcaatgcga
    10861 gcacgccgag cagctgcagc gcggtgaaat tgcgcggctc gcgcgccagc acggaggcgt
    10921 agcctgcgcg cgcctcctcc agcttcccgg cctggtgcag cgcatgggcc tgcgccaggg
    10981 cctcggccgc cccggaggcc ggcgcgggcc gcggcttcca gccgaacagc cgacccagca
    11041 tgcgccgctt cagccgcccg tcttgccggg ttggcgcgcg gccagggtgg cgaccgtcac
    11101 cagctcgtag ctgagcgcgg cggtgaactc gggcgcatag cctgcctcgc gcagcgccca
    11161 gcgcacgcgg ttgccgaggc gggccttctt gtagatgttg agcttgcgcg agcgtgcgaa
    11221 ctggtcgagc cgcgcgaaga tcacgtcgtg ggtcttgcgc agccggtcgg cggtcttgcg
    11281 cgtgtgcggc cccggcgacg gcggcagccg cttcaccagg tcggcgacga tctcgtcggc
    11341 caccgcgtcg acttccttgg tattgaacca gtcacccaac atgagcgcct cgctggcgtg
    11401 atgcaggcgc cattctacgt gatgcgcgcg ccgcaccagg cgcgccacgc ctgccggtag
    11461 gcggcttcca cgtcgcgcgc gaaacgcgcc tcgtccatca gcggggaggc gcgcagccgc
    11521 tggcgcaggg tgccgcgcaa ttcctccagg cgcccggtcg cgccagcgtg cgcgagcgcg
    11581 agtcgcaggt attccgcggg agtggcggcg acccagtcct gcaggccgag cgcgcccagg
    11641 atgctggcag cgctgcgcga ggcggggcgg tcgcccgcca gcgtcagcac cgggaccccc
    11701 atccacagcg tgtcgaaagt ggtggtgccg ccgccatacg gcatgctgtc cagggcgatg
    11761 tccacggcgt cgaactgctc caggtactcg ccgaaggaca cgcgcggcag gatcgtgagc
    11821 cgcgccgccg gcacgccggc ggcggcgaag tcccgcagca ggtccgcgcg tgcgcgtccg
    11881 tcgggcaccc cgaccagccg caggcgcgcc tgcggcagtt gccgcaggat ctcggcccag
    11941 atgcgccgcg acgaggcggt gagcttgggc gcgtggttga acgagccgaa ggtgacgaag
    12001 ccgttgcgcc gccagggcgc ctcggcggca tgcgccgccg catgcagcgg gcggtagcac
    12061 cacaggcaat gcggcagccg cagcagcgat tcggtgtgca ggcgctcgct cgctccgggc
    12121 gggtcggcgc gcgcgtcggt gaggcggtag tcgatgcgcg tcaggccggt gctgtgcagg
    12181 tagccgagcc aggtgaactg cacgggggcc ggccggcgcg cgaacacccc gagccgcagg
    12241 gcgcccgcgt gcccggtgag atcgaccagc acgtcgatcg cgtcgcgctc gatcgcctgc
    12301 gccagcgcct cgtcgctcat ggcccgcgcg tcgcgccagc catccgcggc cgccttcacc
    12361 tgcccggtga cctcgtccac cttgtcgccg gtgctgtaga ggatagcctc gaaccccgcc
    12421 cggtcgtgcc gctccagcag cggcagcagg aaccacgcca ccgggtggcg atagaaatcg
    12481 ctggagacgt agccgatgcg caggcgccga ccgggctcgc gcgacccggc atagggcccg
    12541 gcgtgcgccg gcaccgccgc ctccaggcgt gcgccgaagg cgcgatgccg ctcgtacact
    12601 tcttcggccg acagcgcgtc gcgcagcgtc agcaccagca gttccatcga ttccaggtcg
    12661 aagcgcagcg gctccaggcg gcgcgcggcc gcgaaggccg ccagcgcctc gtcgaggcgg
    12721 gaagtgccgc gcagcatgtt gccgagcagg taccaggcgg gaaccaactc cggctcgatc
    12781 tcgagcgcgc gccgcagggc ggcttcggct tcgtcgtagc ggctctgctt ccacagcagc
    12841 tcgccgtagt tgtaccaggc gccgcccgag gccgggcgca gggcgagcgc ggcctccagg
    12901 ctcgccgtcg ccgcggccag atcgccgcgc gcatcctgca cgtttgccag caccacccgc
    12961 gcgtccgcga aatcgggctt ggccagcagc gcggcgcgca ggagccgctc ggcttcctcc
    13021 tgcgcgccgg cggtgtggcg caggcggccg aggttgtaat tggcataggg atccgccgcc
    13081 cgcacggcga gcagcgcttc gtaggcccgc gtcgcggcgc tggtgtcacc cgccgcctcc
    13141 agcgcggcgc cgaggttgag cagcgccggc gggtagccgg gggcgagcgc cgccgccatg
    13201 cggtagcggc ggcaggcccc tgccaggcgg ccgccgcgct ccgcgcgccc gccgctcgcg
    13261 atcagggcgt ccgcgagccg ccggcgcagc acctacttga ggaccgactt cagcagccgg
    13321 cccatctccg ccgggttcct ggtcaccttg atgccgcagg actccatcac ggcgagtttt
    13381 tcctgcgcgg tgcccttgcc gcccgagatg atcgccccgg cgtggcccat gcgcttgccg
    13441 ggcggcgccg tcacgccggc gatgaagccg accacgggct tccgcatgtt ggccttgacc
    13501 cagcgcgccg cgtcctcctc gtccgagccg ccgatctcgc cgatcatgat caccgcgtcg
    13561 gtgcccggat cgtcgttgaa cagccgcatc acgtcgacgt gcttcatgcc gttcaccggg
    13621 tcgccgccga tgcccactgc gctcgactgg ccgatgccca gctcggtgag ctgccccacc
    13681 gcttcatagg tgagcgtgcc cgagcgcgac accacgccga cgcgccccgg cttgtggatg
    13741 tggcccggca tgatgccgat cttgatctcg cccggcgtga tgacgccggg gcagttgggc
    13801 ccgaccagca gtgtgcggcg cttctgcttc ctcatgcggt cgcgcacctc gatcatgtcg
    13861 cgcaccggga tgccctcggt gatgcagatc accaggtcga ggtccgcttc caccgcctcc
    13921 cagatcgccg cggcggcgaa cggcggcggg acatagatca cggaggtgtt gacgcccgcc
    13981 tcttccttgg cttccttcac cgtgccgaag attgggatgc cctcgaagtt ctcccccgcc
    14041 ttcttcggat tcacgccggc gacgaacgcg tccttgccgc cggcgtaggc gcggctggtc
    14101 tgcgtatgga acatgccggt cttgccggtg atgccctgcg tcaccacccg cgtgtgcttg
    14161 tcgatcagga tgctcatttc ttcttcctcc tggccggcgc cttcttcctc cccggcctgg
    14221 ccttcttctt caccgtcttt ttcttcttcc tgagcgacgc gacgaccttc ttcgccgcat
    14281 cggccatgtt gtccgccgag atgatgggca ggccggagtc tttcagtatc ttgcggccca
    14341 ggtcctcgtt ggtgcctttc atgcgcacca ccagcggcac cgacagcttc acctcgcgcg
    14401 ccgccgccac cacgccggtg gcgatggtgt cgcacttcat gatgccgccg aagatgttga
    14461 ccatgatcgc cttcaccttc gggttggaca gcatgatctt gaaggccgcg gtgaccttct
    14521 cggcggtggc gccgccgccg acgtcgagga agttggccgg cgagccgccg aacagcttga
    14581 tggtgtccat ggtcgccatg gcgaggcccg cgccgttcac catgcagccg atccggccgt
    14641 cgagcgaaat gtagttgagg tcgtacttgg acgcctcgat ctcgagcggg tcctcctcgt
    14701 ccatgtcgcg cagcgccacg atgtcctcgt ggcggaacag cgcgttggag tcgaagttca
    14761 tcttcgagtc gagcaccacc accttgccgc cgccggtcag gatcaggggg ttgatctcgg
    14821 ccagcgacgc atccgtgtcc cagaagcact ggtagagctt cctgaagatc tcggccgcct
    14881 cgcccaccgc cgactcgggc aggccgatct tgcgcgccac gtcggtggcg tccttgtccg
    14941 agaggccggc gaccgggtcg atgaacacct tgtggatctt ctccggcgca tgggcggcga
    15001 cttcctcgat gtccatgccg ccctcggagg aggccatcag cgccacgcgc tgcgtgccgc
    15061 ggtcgaccac catgccgacg tatagctcct tcttgatgtc ggcgccctcc tcgatcagca
    15121 ggcggcgcac cttctggccc tcgggcccgg tctggtgggt cttgagctgc atgccgagga
    15181 tctgcgagga aagcttcttc acctcg
//
LOCUS       DASRIE010000142        10924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3534739, whole genome shotgun sequence.
ACCESSION   DASRIE010000142 DASRIE010000000
VERSION     DASRIE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3534739"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFB53_05975"
     CDS             <1..325
                     /locus_tag="VFB53_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN86318.1"
                     /translation="IALYKEEQARMAADLGKSSRAMILRNHGLLTLGDSVRQAFELMY
                     YLDTACQIQIDACAGGLENVLLMTETAARNTAQQFARDGRPAEQKDWPALLRLLERRG
                     VNYLG"
     gene            complement(309..1094)
                     /locus_tag="VFB53_05980"
     CDS             complement(309..1094)
                     /locus_tag="VFB53_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86319.1"
                     /translation="MPFVTCAGHALEVEWLDAAGPATFTMPTLVFLHEGLGSIRQWRD
                     FPLQVVKATGCRALLYSRYGYGQSEVLREDRAGVRFMHREALEALPELLEQLRIERPV
                     LVGHSDGASIALIHAGAGHPVRALALMAPHVFVEPICVESIRKASASFAASGLAQGLA
                     KYHRDAGKTFHLWADAWLDPAFLQWNIEEYLPRIDCPALAIQGEDDQYGTMRQLERLQ
                     RGLRGPCELVKLAACGHAPFRDQPQATLAAVTRFVRGLANPGS"
     gene            1131..2015
                     /locus_tag="VFB53_05985"
     CDS             1131..2015
                     /locus_tag="VFB53_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN86320.1"
                     /translation="MRFTAWAVLLGTLLLWSGNWIVARAVRNDISPGLATAGRLIIVA
                     CVLSPFVWRGLAAKLPALAPRDWKVLWLLAFAGGGLHLALQWLGLHYTTATSATLYLS
                     TAPVFILLLAAPVLGERIGLLQWLGVAVSFAGVALIGMQGRFVNPSFNVGDLCALASM
                     ALWGAYTVFLRLRRDALDTAEFIAMVCLLGLVFMAPWVAAEAIQGLTLELNAAGAAAV
                     LYSALGSLLLAYAGWSYVVKRLGAARAGVTMHLMPAIAVGLAALFLGERPYWYHGAGV
                     ALILAGVALASMRARAAA"
     gene            2012..2638
                     /locus_tag="VFB53_05990"
     CDS             2012..2638
                     /locus_tag="VFB53_05990"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZN86321.1"
                     /translation="MKPIAFDAALRRRLAVNLARLAPATAGAHALKHAAVGVLVTDAG
                     DGSAALLLTRRAARLKSHAGQYALPGGRVDEDESALEAAVREAREEIGLELPADAFLG
                     RLDDYPTRSGYLITPLVAWVEDPSQLAPNPAEVERIYRLPLAELGKPGSPQFISIPES
                     DRPVIRYPVLGTLLHAPTAAVLYQFMEVAVLGRATRVAHLEQPVWAWR"
     gene            complement(2635..3651)
                     /locus_tag="VFB53_05995"
     CDS             complement(2635..3651)
                     /locus_tag="VFB53_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HZN86322.1"
                     /translation="MNSTVTRRAFLLGMLAAPWAQGAEVAYPRVMRGARLRFPRDHGA
                     HPEFRTEWWYATGWLARGKDPVGFQVTFFRARPRWASDNPSRFAPRQILLAHAAIADP
                     RRGRLRHDQRAARDALGLAGAATRTTEVWIDDWRFELERDTYRARIAAREFSLALALR
                     APRAPLLQGEGGFSRKGHRSEEASYYYSRPQLAASGMLDGEAVAGVAWLDHEWSSQYL
                     APEAAGWDWCGINLADGKSLTAFRIRGRQGGTHYAAPGVAFTARRSWRSPRTGVEYPV
                     AMRVEAQGLAWDLEPLMDDQELDARATAGTVYWEGAVRALQAGKEVGRGYLELTGYWR
                     PMAL"
     gene            complement(3648..6152)
                     /locus_tag="VFB53_06000"
     CDS             complement(3648..6152)
                     /locus_tag="VFB53_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN86323.1"
                     /translation="MRLFLALNARFAHRRARTLLAVAGVALGVALGFGVHLVNRAAIE
                     DLAAGVRALAGEADLEVRGGRGGFPEAVYAAVARSPGVALASPVLELEAGLASGAGLP
                     ALRIVGLDPLRASQLQPQLFAQQPQLMAELFRPDTVLLSGAAAETLRLGKGGSLTLLV
                     GLQAVELRVAGTLPASSLPGIAALTDISTAQWRLARLGELNRIGLRLAPGADRAQAER
                     QIAALLPAGVHVAAPEVAERSGATLSRAYRVNLNALALVALFTGGFLVFSVQALEVAR
                     RRGEHALLRVLGLERAGVARLVLAEAAAVGAAGAALGLACGYGFAALALRASGGDLGA
                     GMFRGLAPQAGFAPAAALLYLCAGIVVAMAGALLPALEAGRTPPARALKAGDEQQLFA
                     RALSLKPALALLLAALLLAFLPPVNGLPLAGYACIACLLVGGILLMPALSRRVFLALP
                     LSGRPSTALAAAQLRGAPGQAMVSLAAIVASFSLMVAMAIMVSSFRGSVDRWLDAVLP
                     ADLYLRTTHAGETAFLEPRFEERVKRLPQVARAEFLRSARATLDPARPAVSLLARDVP
                     AAGLASVLPLTGAVHEPRADDPPAAWVSEAVAQLYGAQPGRRLQLPIAGKPQEFVVAG
                     VWRDYARQHGAIVIERPVYARLTGDARSNDAALWLAPGARAGEAERAIRRLRGAEHLE
                     FAQPGEIRELSMAIFDRSFAITYAMEAVAVLVGLCGLSSALGAIVLSRRREFGMLRHL
                     GMTRGEIGAMLAKEGGLLALLGAAAGLALGALISLVLIYVVNLQSFHWSMDLRLPWLA
                     LAGLTALLVALAALTAWLSGREAMGMTPVRAVKEDW"
     gene            6204..7667
                     /locus_tag="VFB53_06005"
     CDS             6204..7667
                     /locus_tag="VFB53_06005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZN86324.1"
                     /translation="MAERADTFDEELLRTTVHVARIGICFIGPDGLFVFVNPAFCELC
                     GFQPAELIGRHWTLAAPPAIVERQERFRQALLEDSPRVPLEWKIRRKDGSLFDALVSW
                     KALDGRDGRRYVVVTFSDITERTKQLQLSEERYRQVVENAYEGIIVTQEGYVKFANAR
                     AAALSGRALEDTYTTPFPEMIHPEDRPRVYQNYLRRMRGEPTENNYDFRVLTPGGEAR
                     WVTISAVAIEWEGKPATLNFLTDATARKQAEEEMRRALEQERELSELKSRFVALTSHE
                     FKTPLATILSSIELLEDFGEKMPAAQRREVTQLVKAAVQRMTGMLDQVLLIGRAESQG
                     LEFQPQPLDARAFCEQLIEEARAAGGPGITFLRRGRAGRRLLDEKLLRHALGNLLSNA
                     IKFSRGRKVTLELDADEHALQFRVRDQGIGIPEADRARLFATFHRGANAGDVEGTGLG
                     LAIVKQCVERHGGSVGVESALDSGTTFTVTIPAAPAA"
     gene            7664..9022
                     /locus_tag="VFB53_06010"
     CDS             7664..9022
                     /locus_tag="VFB53_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN86325.1"
                     /translation="MTRPGAAARRILIVDDDPKLLRLLALRLVNEGYRVAEAKSGEQA
                     LAQLGAGLPDLIITDMKMGGMDGLQLFEQVQRQHPSLPVIILTAHGSIPDAVSATRRG
                     VFGYLAKPFEAKDLMREVQRALAQAAPAAGPESGWRREILTRSPAMESVLADARLVAA
                     SDASVLIQGASGTGKELLARAIHAASHRYGRPLVALNCGALPEPLLESELFGHVKGAF
                     TGAVREHQGLFQSAQGGTLFLDEIGDMPLALQVKLLRALQERQVRPVGGTQSVDVDVR
                     VISATHRDLREEIAAARFREDLYYRLNVVNLELPALAARREDIPLLAAHFLARLAARY
                     GKPIAGCAPEAVQLLLTAPWPGNVRQLHNVIEKCVALSTGTLLSAALVERALAAPGDD
                     FEAFDDARRQFEREYLTRILRITDGNVTQAAKLAKRNRSDFYSLLARHELDPATFKPG
                     KA"
     gene            9224..9655
                     /locus_tag="VFB53_06015"
     CDS             9224..9655
                     /locus_tag="VFB53_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HZN86326.1"
                     /translation="MTPRQIELVQESFTKVLPIAPAAAEIFYARLFEIDPALRGLFKG
                     DMKEQGRMLMMMIGGAVRGLANPPALIPVLKDLGARHLGYGVEERHYDTVGTALIWTL
                     QKGLGEAFTDQVCEAWVAAYQLLAGVMQQGAREARQMKKAA"
     gene            9661..10464
                     /locus_tag="VFB53_06020"
     CDS             9661..10464
                     /locus_tag="VFB53_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86327.1"
                     /translation="MRGCQSSGRGAGVVLLHSSLSTGAQWKRLAEQISPSYHVLALDL
                     LGCGAAPAVADPEGYSLRDESARILELADAWFGVGARFHLVGHSYGGLAALALARENP
                     ARVAGLAVYEPVCFNLAGAGDPDLAFLRRTAATVAMHVRAGRLIEATGLFFDYWNGPG
                     AFRLLPPAAQARCVQGIAKVPLDFQAAFQEPGLAQDYASISAPTLLMGGTQSPRLTRG
                     LLRTLAGAIPNAALAWVEGGHMAPLSAPGQVNLLLGSFLDWTAPTARAA"
     gene            10561..>10924
                     /locus_tag="VFB53_06025"
     CDS             10561..>10924
                     /locus_tag="VFB53_06025"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HZN86328.1"
                     /translation="MKKTLLLAAGLFVSTQVFPQAAFPPPPPKVSPAIGVIDMHTHTH
                     PDVFGRNMDDVDLAQIARARGMRGIVIKNHISETASRAALAMKAVPGIEVFGGIVLNN
                     AVGGINPNAVEWMHRFYGS"
BASE COUNT         1555 a         4031 c         3884 g         1454 t
ORIGIN      
        1 catcgcgctg tacaaggagg agcaggcgcg catggccgcc gacctcggca agagctcgcg
       61 cgccatgatc ctgcgcaacc acggcctgct caccctgggc gactcggtgc gccaggcctt
      121 cgagctgatg tactacctcg acaccgcctg ccagatccag atcgacgcct gcgccggcgg
      181 cctggagaac gtgctgctca tgaccgaaac cgcggcgcgg aacacggcgc agcagttcgc
      241 gcgcgacggg cgccccgccg agcagaaaga ctggccggcg ctgctgcggc tgctcgagcg
      301 ccgcggcgtc aactacctgg gttagccagg ccgcgcacga agcgcgtcac ggcggccagc
      361 gtcgcctgcg gctggtcgcg gaacggcgcg tgcccgcagg cggccagctt caccagctcg
      421 cagggaccgc gcaggccgcg ctgcaggcgc tccagctgcc gcatggtgcc gtactggtcg
      481 tcttccccct ggatcgcaag cgccggacag tcgatgcgcg gcagatattc ctcgatgttc
      541 cattgcagga acgccgggtc gagccaggca tccgcccaca agtggaaggt cttgcctgcg
      601 tcgcgatggt acttggcgag gccctgcgcc aggccggagg cggcgaagct tgcgcttgcc
      661 ttgcggatgc tctcgacgca gatcggctcg acgaacacgt gcggcgccat cagtgccagg
      721 gcgcgcaccg gatgcccggc gccggcgtgg atcagggcga tggaggcgcc gtcggagtgc
      781 cccaccagga ccggccgctc gatgcgcagc tgctccagca gctcgggcag cgcctcgagc
      841 gcctcgcggt gcatgaagcg cacgcccgcc cggtcctccc gcagcacctc ggactggccg
      901 tagccgtagc ggctgtagag cagcgcgcgg cagcccgtgg ccttcaccac ctgcagcggg
      961 aaatcgcgcc actggcggat cgaccccagc ccctcgtgca ggaacaccag cgtcggcatc
     1021 gtgaacgtgg cgggcccggc ggcgtccagc cactcgacct cgagcgcatg gccggcgcag
     1081 gtcacgaaag gcatcggaac aatctagcac gcaggcgata atcgccgcct atgcgtttca
     1141 ccgcctgggc cgtgctgctg ggaacgctcc tattgtggtc cggcaactgg atcgtggcgc
     1201 gcgccgtgcg caacgacatc tcgcccggcc tcgccaccgc cgggcgcctg atcatcgtcg
     1261 cctgcgtgct ctcccctttc gtctggcgcg gcctcgcggc gaagctcccc gcgctcgcgc
     1321 cgcgcgactg gaaggttctg tggctgctcg ccttcgcagg cggcggcctg cacctcgccc
     1381 tgcaatggct ggggctgcac tacaccaccg ccaccagcgc gacgctgtac ctgtccaccg
     1441 cgccggtgtt catcctgctg ctcgccgcgc cggtgctcgg cgagcgcatc ggcctgctgc
     1501 agtggctggg cgtggcggtc tcgttcgcgg gcgtggccct gatcggcatg caggggcgct
     1561 tcgtcaaccc gagcttcaac gtcggcgacc tgtgcgccct cgcatcgatg gcgctgtggg
     1621 gcgcctacac cgtgttcctg cgcctgcggc gcgacgcgct cgacacggcg gaattcatcg
     1681 ccatggtctg cctgctcggc ctcgtcttca tggcgccctg ggtggcggcg gaggcgatcc
     1741 agggcttgac gctcgagctg aacgcggcgg gcgcggcggc ggtgctgtac tccgccctcg
     1801 gctccctcct cctcgcttac gccggatgga gctacgtcgt gaaacggctg ggcgcggcgc
     1861 gcgccggcgt cacgatgcac ttgatgcccg cgatcgcggt ggggctcgcc gcgctgttcc
     1921 tcggggagcg gccctactgg taccacggcg cgggcgtcgc cctgatcctc gccggcgtgg
     1981 ccctcgcttc gatgcgcgcg cgggcggcgg cttgaagccg atcgccttcg acgccgcgct
     2041 gcgccggcgc ctcgccgtca atctcgcgcg tcttgcgccc gccaccgccg gcgcgcacgc
     2101 gctcaagcac gccgcggtcg gcgttctcgt caccgacgcg ggcgacggca gcgcggcctt
     2161 gctgctcacg cggcgcgcgg cgcgcctgaa atcgcacgcc ggccagtacg ccctgcccgg
     2221 cgggcgggtg gacgaggacg aatcggcgct ggaagccgcg gtgcgcgaag cgcgcgagga
     2281 gatcggcctg gaacttccgg ccgacgcctt cctcggacgg ctcgatgact atccgacgcg
     2341 ctcgggctac ctcatcaccc cgctcgtcgc ctgggtcgag gatccgtcgc agctggcgcc
     2401 caatccggcg gaagtggagc gcatctaccg cctgccgctc gccgagctcg gcaagccggg
     2461 ctcgccgcag ttcatttcga tcccggaaag cgaccgcccg gtgatccgct acccggtgct
     2521 cggcacgctg ctgcacgcgc cgaccgccgc ggtgctctac cagttcatgg aagtcgccgt
     2581 gctcggccgc gcgacgcgcg tcgcgcatct cgagcagccg gtgtgggcct ggcgctagag
     2641 cgccatcggc cgccagtagc cggtcagctc gaggtagccg cgcccgactt ccttgcccgc
     2701 ctgcagcgcg cgcaccgcgc cttcccagta caccgtgcct gcggtggcgc gcgcgtcgag
     2761 ctcctggtcg tccatcagcg gctcgaggtc ccaggccagg ccctgggcct cgacgcgcat
     2821 cgccaccgga tactcgacgc cggtgcgcgg agagcgccac gacctgcgcg cggtgaacgc
     2881 cacccccggc gcggcgtaat gcgtgccgcc ctgccggccg cggatgcgga acgcggtgag
     2941 cgatttgccg tccgcgagat tgatgccgca ccagtcccag cccgcggcct cgggcgcgag
     3001 gtactggctg gaccactcgt ggtccagcca ggcgacgccc gccacggcct cgccgtccag
     3061 catgccgctc gccgccagct gcgggcggct gtagtagtag ctcgcctctt cgctgcggtg
     3121 gcccttgcgg ctaaagccgc cctcgccctg cagcagcggt gcgcgcggcg cgcgcagcgc
     3181 gagcgccagg gagaattcgc gcgccgcgat gcgcgcgcgg taggtatcgc gctccagctc
     3241 gaagcgccag tcgtcgatcc acacttcggt ggtgcgcgtc gcggcgccgg caaggcccag
     3301 cgcgtcgcgc gccgcgcgct ggtcgtggcg cagccgcccg cgccgcgggt cggcgatggc
     3361 ggcatgcgcc agcaggatct ggcgcggcgc gaaccggctc gggttgtcgc tcgcccagcg
     3421 cggccgcgcg cggaaaaacg tgacctggaa gccgaccggg tccttgccgc gcgccagcca
     3481 gccggtcgca taccaccatt cggtgcggaa ttcgggatgc gcgccgtgat cgcgcggaaa
     3541 gcgcagccgc gccccgcgca tcaccctggg ataggcgacc tcggcgccct gcgcccaggg
     3601 cgcagccagc atgccgagca ggaacgcgcg tcgcgtcacg gtcgaattca ccagtcctcc
     3661 ttgaccgcgc gcaccggcgt catgcccatc gcctcgcgcc ccgacagcca ggcggtgagc
     3721 gcagcgagcg ccaccagcag cgcggtgagc cccgccagcg cgagccaggg caggcgcagg
     3781 tccatgctcc agtggaagga ctgcaggttg acgacgtaga tcaggaccag gctgatgagc
     3841 gcgcccagcg ccaggcccgc ggcggcgccg agcagcgcca gcaggccgcc ttccttggcg
     3901 agcatggcgc cgatttcgcc gcgcgtcatg ccgaggtggc gcagcatgcc gaactcgcgc
     3961 cggcgcgaga gcacgatggc gccgagggcc gaggacaggc cgcacagtcc caccagcacc
     4021 gccaccgctt ccatcgcgta ggtgatggcg aaactgcggt cgaagatcgc catcgacagc
     4081 tcgcggattt cccccggctg cgcaaactcg aggtgctccg ccccgcgcag gcgccggatg
     4141 gcccgctccg cctcgccggc gcgcgccccc ggcgccagcc acaaggcggc gtcgttgctg
     4201 cgcgcgtcgc cggtcagccg cgcgtacacc ggcctctcga tgacgatggc gccgtgctgg
     4261 cgcgcgtaat cgcgccacac gccggcgacc acgaactcct gcggcttgcc cgcgatgggc
     4321 agctgcagcc gccggccggg ctgcgcgccg tagagctgcg ccactgcctc gctgacccac
     4381 gcggcgggcg gatcgtccgc gcgcggctcg tggacggcgc ccgtcagcgg cagcacgctc
     4441 gccaggcccg ccgcgggcac gtcgcgcgcg agcagcgaca ccgccgggcg cgcggggtcg
     4501 agcgtcgcac gcgcgctgcg caggaactcg gcgcgcgcga cctgcggcag gcgtttcacg
     4561 cgctcctcga acctcggctc gaggaaggcg gtctccccgg cgtgcgtggt gcgcagatac
     4621 aggtccgccg gcagcaccgc atcgagccag cggtcgaccg agccgcggaa cgaggacacc
     4681 atgatcgcca tcgccaccat cagcgagaag ctggccacga tcgcggccag gctcaccatc
     4741 gcctggcccg gcgcgccgcg cagctgggcg gcggcgagcg cggtggacgg gcgcccgctg
     4801 agcggcaggg cgaggaagac tctgcgcgac agcgccggca tcagcaggat gccgcccacc
     4861 agcaggcagg cgatgcaggc atagccggcg agcggcagcc cgttcaccgg cggcaggaac
     4921 gcgagcagca gcgccgccag cagcagcgcc agcgcgggtt tgagcgacag cgcgcgcgcg
     4981 aacagctgct gctcgtcgcc cgccttcagc gcccgcgccg ggggcgtgcg gcccgcttcc
     5041 agcgcgggca gcagcgcgcc cgccatcgcc acgacgatgc cggcgcacag gtagaggagc
     5101 gccgccgccg gcgcgaaacc cgcctgcggc gcgaggccgc ggaacatccc ggcgcccagg
     5161 tctccgcccg aggcgcgcag cgccagcgcc gcgaacccgt agccgcaggc gaggccaagc
     5221 gctgcgccgg cggcaccgac cgccgcggcc tccgccagca ccagccgcgc gacgcccgcg
     5281 cgttccaggc cgagcacgcg cagcagcgcg tgctcgccgc gccggcgcgc cacctccagc
     5341 gcctgcacgg agaacaccag gaatccgccg gtgaacaggg ccaccagcgc cagcgcgttc
     5401 aggttgacgc ggtaggcgcg cgacagcgtg gcgccgctgc gctccgcgac ctccggtgcc
     5461 gcgacgtgga cccccgcggg cagcagcgcc gcgatctggc gctcggcctg cgcgcggtcc
     5521 gcgcccggcg ccagccgcag tccgatgcgg ttcagctctc ccagccgcgc gaggcgccac
     5581 tgcgcggtgg agatgtcggt gagcgcggcg atgccgggca ggctcgaggc gggcagcgtg
     5641 ccggcgaccc gcagttccac ggcctgcagg ccgaccagca gcgtcaggct gccgcccttt
     5701 cccaggcgca gcgtctcggc ggcggcgccg gacaggagca cggtgtcggg cctgaacagt
     5761 tcggccatca gctgcggctg ctgcgcgaag agttgcggct gcagctgcga agcgcgcagc
     5821 ggatcgagtc cgacgatgcg cagcgcggga agcccggcgc cgctggcgag gccggcttcc
     5881 agctcgagca ccgggctggc gagcgccacg ccggggctgc gcgccaccgc agcgtacacg
     5941 gcctccggga acccgccgcg cccgccgcgc acctcgaggt cggcctcgcc ggcgagggcg
     6001 cgcacgccgg cggcgaggtc ctcgatcgcg gcgcggttca ccaggtgcac gccgaagccc
     6061 agcgccacgc cgagcgccac gccggctacc gcgagcaggg tgcgcgcgcg gcggtgcgcg
     6121 aagcgcgcat tgagggcgag gaagagtcgc acgaggcgga tgcgccaaag tatagcggtt
     6181 acgcggttac aatcgccttc cccatggccg agcgcgcaga caccttcgac gaagagctgc
     6241 tgcgcaccac ggtgcacgtc gcgcgcatcg gcatctgctt catcggaccg gacgggctgt
     6301 tcgtcttcgt caacccggcg ttctgcgagc tgtgcggctt ccagcccgcc gagctcatcg
     6361 gccggcactg gaccctggcc gccccgcccg cgatcgtcga gcgccaggag cggttccgcc
     6421 aggcgctgct ggaggactcg ccccgggtgc cgctggaatg gaagatccgc cgcaaggacg
     6481 gcagcctgtt cgacgcgctg gtgagctgga aggcgctcga cgggcgcgac ggccgccgct
     6541 acgtggtggt gaccttctcg gacatcaccg agcgcaccaa gcagctgcag ctctccgagg
     6601 agcgctaccg ccaggtggtg gagaacgcct acgaaggcat catcgtcacg caggagggct
     6661 acgtgaagtt cgccaacgcg cgcgccgcgg cgctctccgg ccgcgcgctc gaggacacct
     6721 acaccacgcc gttcccggag atgatccatc cggaggaccg gccgcgcgtc taccagaatt
     6781 acctgcggcg catgcgcggc gagcccaccg agaacaacta cgacttccgc gtgctgacgc
     6841 ccgggggcga ggcgcgctgg gtgacgatca gcgcggtggc catcgaatgg gaaggcaagc
     6901 cggcgacgct caacttcctc accgacgcca cggcgcgcaa gcaggccgag gaggaaatgc
     6961 ggcgcgcgct ggagcaggag cgcgagctct cggagctgaa atcgcgcttc gtcgcgctga
     7021 cctcgcacga gttcaagacc cccctggcga cgatcctgtc ctccatcgag ctgctggagg
     7081 acttcggcga gaagatgccc gcggcgcagc ggcgcgaggt gacgcagctg gtgaaggcgg
     7141 cggtgcagcg catgaccggc atgctcgacc aggtgctgct catcggccgc gcggagtcgc
     7201 agggcctgga attccagccg cagccgctcg acgcgcgcgc cttctgcgag cagctgatcg
     7261 aggaggcgcg cgccgccggc ggcccgggca tcactttcct gcgccgcggc cgcgccgggc
     7321 ggcgcctgct cgacgagaag ctgctgcgcc acgcgctggg caacctgctg tccaacgcca
     7381 tcaagttttc gcgcggccgc aaggtgacgc tcgagctgga cgccgacgag cacgcgctgc
     7441 agttccgcgt gcgcgaccag ggcatcggca ttccggaggc cgaccgcgcg cgcctgttcg
     7501 ccaccttcca ccgcggcgcc aacgccggcg acgtcgaggg cacggggctc ggcctcgcca
     7561 tcgtcaagca gtgcgtcgag cggcacggcg gctcggtcgg cgtggagagc gcgctggaca
     7621 gcggcaccac cttcaccgtc accatccccg ccgcgccggc cgcatgacgc gccctggcgc
     7681 cgcggcgcga cgcatcctga tcgtcgacga cgaccccaag ctgctgcgcc tgctcgccct
     7741 gcgcctggtg aacgagggct accgggtggc cgaggcgaag agcggcgagc aggcgctcgc
     7801 gcagctcggc gcggggctgc cggacctgat catcaccgac atgaagatgg gcggcatgga
     7861 cgggctgcag ctgttcgagc aggtgcagcg gcagcacccc tccctgccgg tgatcatcct
     7921 caccgcgcac ggcagcattc ccgacgcggt ctccgccacg cgccgcggcg tgttcggcta
     7981 tctcgccaag cccttcgagg cgaaggacct gatgcgcgag gtgcagcgcg cgctggcgca
     8041 ggccgcgccc gccgcgggcc ccgagtcggg gtggcgccgc gagatcctca cccgcagccc
     8101 ggcgatggag agcgtgctgg cggacgcgcg cctggtggcg gcgagcgacg ccagcgtgct
     8161 gatccagggc gccagcggca ccggcaagga actgctggcg cgcgccatcc atgccgccag
     8221 ccaccgttac ggccggccgc tggtggcgct caactgcggc gcgctgcccg agccgctgct
     8281 cgaatcggag ctgttcggcc acgtgaaggg cgccttcacc ggggcggtgc gcgagcacca
     8341 ggggctgttc cagtcggcgc agggcggcac gctgttcctc gacgagatcg gcgacatgcc
     8401 gctcgcgctg caggtgaagc tgctgcgcgc gctgcaggag cgccaggtgc ggccggtggg
     8461 cggcacgcag tcggtggacg tcgacgtgcg cgtgatctcg gcgacgcacc gcgacctgcg
     8521 cgaggagatc gccgccgcgc gcttccgcga agacctgtac taccgcctca acgtggtgaa
     8581 tctggagctg cccgcgctcg ccgcgcggcg cgaggacatc ccgctgctcg ccgcgcattt
     8641 cctcgcgcgg ctcgccgcgc gctacggcaa gccgatcgcg ggctgcgcgc cggaggcggt
     8701 gcagctgctg ctcaccgcgc cctggcccgg caacgtgcgc cagctgcaca acgtgatcga
     8761 gaaatgcgtg gcgctgtcca ccggcacgct gctcagcgcg gcgctggtgg agcgcgcgct
     8821 cgccgccccc ggcgacgatt tcgaggcctt cgacgacgcg cgccgccagt tcgagcgcga
     8881 gtacctgacg cgcatcctgc gcatcaccga cggcaacgtc acccaggcgg ccaagctcgc
     8941 caagcgcaac cgcagcgact tctattcgct gctggcgcgc cacgagctcg atcccgcgac
     9001 gttcaagccc ggcaaggcct agcgcctccc tgttggcgtt tgccaacgga ggttttccgc
     9061 ttagtttcaa tgacttgcag aaggggtgac gggctgccgt tggtgtttgc caacacccgc
     9121 ggcccgccag atatgagcta acccattgat tttgcttgga acactgatct ggcacgggcc
     9181 ctgcaatagg gatggcattg ccacccattg aaggagaagg cgcatgaccc cccgtcaaat
     9241 cgagctggtg caggagagct tcaccaaggt gctgccgatc gccccggccg ccgcggagat
     9301 cttctacgcg cggctgttcg agatcgaccc cgcgctgcgc ggcctgttca agggcgacat
     9361 gaaggagcag ggccgcatgc tgatgatgat gatcggcggc gcggtgcgcg gcctggccaa
     9421 tcccccggcg ctgatcccgg tgctgaagga cctcggcgcg cgccacctcg gctacggcgt
     9481 cgaggagcgg cattacgaca cggtgggcac cgcgctcatc tggacgctgc agaagggcct
     9541 gggcgaagcc ttcaccgacc aggtgtgcga ggcctgggtc gcggcctatc agctgctcgc
     9601 cggcgtgatg cagcagggcg cgcgcgaagc gcggcagatg aagaaggcgg cttgagcgcc
     9661 atgcgcggct gccagtcctc gggtcgcggc gcgggagtgg tgctgctgca ctcctcgctg
     9721 tccaccggcg cgcaatggaa gcgcctcgcg gagcagatct cgcccagcta tcacgtgctc
     9781 gcgctggacc tgctcggctg cggcgcggcg cccgccgtgg cggaccccga gggctacagc
     9841 ctgcgcgacg agagcgcgcg catcctcgag ctggcagacg cctggttcgg ggtcggcgcg
     9901 cgcttccacc tggtcgggca ttcctacggc ggcctcgccg cgctggcgct cgcgcgcgag
     9961 aatcccgcgc gcgtcgcagg cctcgcggtg tacgaaccgg tgtgcttcaa cctcgccggc
    10021 gccggcgatc cggacctggc gttcctgcgc aggaccgccg ccacggtggc catgcacgtg
    10081 cgcgccgggc gcctgatcga ggcgacgggg ctgttcttcg actactggaa cggccccggc
    10141 gcgttccggc tcctgccgcc cgcggcgcag gcgcgctgcg tgcagggcat cgccaaggtg
    10201 ccgctcgatt tccaggccgc gttccaggag ccgggcctgg cgcaggacta cgcgtcgatc
    10261 agcgcgccga cgctgctcat gggcggcacg cagagcccgc gcctgacgcg cggcctgctg
    10321 cgcacgctcg ccggggcgat tcccaacgcg gcactggcct gggtggaagg cggccacatg
    10381 gcgccgctgt ccgcgcccgg gcaggtcaac ctgctgctgg gctcgttcct cgactggacg
    10441 gcgccgaccg cgcgcgcggc ctgaggccag cgtagcgtcg gctccgattg ccgcaccgca
    10501 ggaagactgg cgcccggtgc cggcgtagga tctcgcccct gttcaccagg gagagcggat
    10561 atgaagaaga cattgctgtt ggctgccggc ctgttcgttt ccacgcaggt tttcccgcag
    10621 gcggccttcc ctcctcctcc gcccaaggtc agcccggcga tcggcgtgat cgacatgcac
    10681 acgcatacac accccgacgt cttcggccgc aacatggacg acgttgacct cgcccagatc
    10741 gccagggcgc gcggcatgcg cggcatcgtg atcaagaacc atatcagcga gacggcgagc
    10801 cgcgccgcgc tcgccatgaa agcggtcccg ggtatcgagg ttttcggcgg catcgtgctg
    10861 aacaacgcgg tgggcggcat caacccgaac gccgtggagt ggatgcaccg cttctacggc
    10921 agcc
//
LOCUS       DASRIE010000143         7074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2216198, whole genome shotgun sequence.
ACCESSION   DASRIE010000143 DASRIE010000000
VERSION     DASRIE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216198"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1803
                     /locus_tag="VFB53_06030"
     CDS             94..1803
                     /locus_tag="VFB53_06030"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HZN86329.1"
                     /translation="MTTASDGPRGLARGLTNYGDPDFALYLRRSFATSMGYSKAMLAR
                     PVVGIAQSASGFNNCHRHFPELIDAVKRGVLAAGGLPIDFPTISLGEVFLSPTSMMFR
                     NLMAIDVEEMIRAQPMDAVVLVGGCDKTVPAQLMGAASADVPAVQLLAGPMMPMAYRG
                     ERLGACTDCRRFWAKYRAGGVTADEIERIEENLATTAGTCAVMGTASTMASIAEALGM
                     ALPGTAAIPAVHADRLRAAEASGRRAVELVRNPIRPSQVITARAVENAIRVLLAIGGS
                     TNAIVHLTAIAGRAGVKVDLHRLNELSDSTPVLVDLKPTGQFYMSDLYAAGGVGAVLR
                     ELKPLLHLDCLTVAGETLGERLAHETGAWVDRAVVRPFAEPLAKEGGLVALFGSLAPN
                     GAILKRSAADPRLFEKEGRAVVFTSLDDLAARVDDPALDVTPEDFMVLQNAGPKSGYA
                     MPEAGYLPIPAKLARAGLKDMVRISDARMSGTAYGTIVLHVSPEAATGGPLALVRNGD
                     RIRLSVKSRRIDLQVPGEELARRRAAWQPPVAPPARGYAKLYMDHVLQAEHGCDFDFL
                     RKD"
     gene            1814..2881
                     /locus_tag="VFB53_06035"
     CDS             1814..2881
                     /locus_tag="VFB53_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZN86330.1"
                     /translation="MSSARSREHRKAATRAGLRYVTDGFAGVTRKRSGTGWTYYAPGG
                     ARIADAATRKRLNALAIPPAWTDVWICPDPGGHIQATARDARGRKQYRYHPSYREARD
                     QSKFRRMLEFSEALPQLRERVERDLRAEDLSRRQLLATVVRLLDRTLIRVGNDEYARE
                     NQSFGLTTLRRRHVQVEGDRLRFAFRGKSGVEHSISVADPRLARIIQRCQDLPGQEMF
                     QYLGADKKRQTVSSDDVNGYLREASDRDVTAKDFRTWGGTMLAAVELRAMGPAASRRE
                     ADRNILRALDAVAERLGNTRTVCRKYYVHPALLQAYLVGRTAPWPPAADKRAKRRAKP
                     GGALRRDEVAVLQFLQERVPD"
     gene            2878..3636
                     /locus_tag="VFB53_06040"
     CDS             2878..3636
                     /locus_tag="VFB53_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN86331.1"
                     /translation="MSGAFQLADVGAAQYLLIGAVAFAASILGGLAGYGTGLVVPIFL
                     APVVGIANVVPVMAVGMIFANGSRVAAFWRDIQWPHARRILLFGLPACVAGAYGYTLL
                     AANWVALLLGVFFIASVPLRRALVRAGWTLEGRGVAAAGAGFGFVNGGVPGAGLLLIS
                     TLMAAGLAGPALIATDAIVSIIMGAAKIALFGSVARLDAGLALAGVLIGLCTVPGAFV
                     ARRLLARISLKVHTVVMEIVVLAGGAAFLWRAVA"
     gene            3633..3998
                     /locus_tag="VFB53_06045"
     CDS             3633..3998
                     /locus_tag="VFB53_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZN86332.1"
                     /translation="MKDESQAIGGYLARVPEARRERALALHGLILSLFPQAEVSLEQR
                     MPTYRLGEEWIAWASQKGHLALYTCAEDRVAGFRARHPEVDAGEGCLRFRDSQRLPLA
                     DLAQVVRRALAPELARRRR"
     gene            4032..4499
                     /locus_tag="VFB53_06050"
     CDS             4032..4499
                     /locus_tag="VFB53_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN86333.1"
                     /translation="MDRETRATVEDHRALRLWLRLLTCSQLIERQVRSRLRERFDTTL
                     PRFDLMSQLERHPAGLKMNELSRLLMVTGGNVTGIVDQLVKEGLVERVDDAADRRAWR
                     VRLTRAGDKAFAEMARAHEEWVAELLSGVSRRDSDALMQLLARLKQATLEKSA"
     gene            4496..5287
                     /locus_tag="VFB53_06055"
     CDS             4496..5287
                     /locus_tag="VFB53_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZN86334.1"
                     /translation="MIRTQHFLWRVDGKVGVLTLNRPEKKNPLTFESYAELRALFQGL
                     QQESEVKAVVLTGAGGNFCSGGDVHEIIGPLTKMQPPQLLEFTRMTGDVVKAMRACPQ
                     PIVAAVEGVCAGAGAMLALFSDLRYGKPDAQTAFLFTRVGLAGADMGACAMLPRVIGQ
                     GRAAELLYSGRAMTAEEGLAWGFFNRLADPVLPAAMEIAQKLASGPTFAHGVTKKMLH
                     QEWNAGLDEAIEMEAQAQAICMQTKDFQRAYEAFAAKQKPKFEGD"
     gene            5287..6390
                     /locus_tag="VFB53_06060"
     CDS             5287..6390
                     /locus_tag="VFB53_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN86335.1"
                     /translation="MDRFSHLQWPFLDPLHATLSRAVDAWALEKLDYAHGEDADAVCR
                     RLVKDLGQAGYLKYCVNENPDVRSIALIREVLSYHAGLADFSFVMQGLGSGPIVLAGS
                     DAQKQKYLPAAAAGEAIAAFALSEPDAGSDVQAMTATAQRSGAHWVLEGTKTWISNGG
                     IADFYVAFAKADEGISAFVVDAKEVDASERIEIVAPHPMATVRFKGSKGELVGEAGQG
                     FKLAMRNLDVFRTTVAAAAVGFARRAFDEALARVKQRRMFGQRLADLQMTQAKLADMA
                     TAIDAAALLTYRAAWLRDVKRQRVTREAAMAKMQATEAAQAVIDDAVQLLGGLGVMRG
                     HPVERLYREVRALRIYEGATEVQKVIIARELLK"
     gene            6387..>7074
                     /locus_tag="VFB53_06065"
     CDS             6387..>7074
                     /locus_tag="VFB53_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN86336.1"
                     /translation="MKWKSAHLDSFALDNLPPRGQWPEFLFERPEFRYPGRMNCATEL
                     LDKMVSRGFGHRPLIRTPEGGCTYTELYAQANRIANVLVRDMGLQPGNRVLLRGPNSP
                     MMAACWFAVMKAGAVCVATMPLLRAKELTEIVQKAQVGFALCDRRLAAELEAALPACP
                     TLQSVKYWHDDAKDSLDGLCVRAPQVFANVDTAAEDVALIAFTSGTTGKPKGTMHFHR
                     DVVAMCDCFPR"
BASE COUNT         1037 a         2432 c         2579 g         1026 t
ORIGIN      
        1 ccgacggcgg tcagaaggtc gcggcgggag ccttcgccaa gagcgcgggc ttgcgtccgg
       61 gcgcgcggct cggcgcggcc tgagccgggc ggcatgacga cggcatcgga cggcccccgc
      121 gggctcgcac gcggactcac caactacggc gatcccgatt tcgcgcttta cctgcgccgc
      181 tcgttcgcga cgtcgatggg gtattcgaag gcgatgctgg cgcgcccggt cgtcggcatc
      241 gcgcagtcgg cgagcggctt caacaactgc caccgccact tccccgagct gatcgacgcc
      301 gtgaagcgcg gggtgctggc cgccggcggg ctcccgatcg atttccccac gatctcgctc
      361 ggcgaggtgt tcctctcgcc gaccagcatg atgttccgca acctcatggc gatcgacgtc
      421 gaggagatga tccgcgcgca gccgatggac gcggtggtgc tggtcggcgg ctgcgacaag
      481 acggtgcccg cgcagctcat gggcgccgcg tcggccgacg tgcccgcggt gcagctcctg
      541 gccgggccga tgatgccgat ggcgtatcgc ggcgagcggc tcggcgcgtg caccgactgc
      601 cggcgcttct gggcgaagta ccgcgccggc ggcgtcaccg cggacgagat cgagcgcatc
      661 gaggagaacc tggcgacgac cgcgggcacg tgcgcagtga tgggcaccgc gagcacgatg
      721 gcgtcgatcg ccgaagcgct gggcatggcg ctgcccggga cggccgcgat ccctgcggtg
      781 cacgccgacc ggttgcgcgc agcggaagcg agcgggcggc gcgcggtcga gctggtcagg
      841 aacccgatcc ggccgtccca ggtaatcacg gcgcgagcgg tggaaaacgc catccgcgtg
      901 ctgctcgcca tcggcggctc gaccaatgcc atcgtgcacc tcacggcgat cgcgggccgc
      961 gcgggggtga aggtggacct gcatcgcctc aacgagctct ccgactcgac gccggtgctg
     1021 gtggacctga aacccaccgg ccagttctac atgtccgacc tgtacgccgc gggcggcgtg
     1081 ggcgcggtgc tgcgggagtt gaagcccctg ctgcacctgg attgcctgac ggtggcgggc
     1141 gagacgctcg gcgagcgcct cgcgcacgag acgggcgcct gggtggaccg ggcggtggtg
     1201 cggccgttcg ccgagccgct ggcgaaggag ggcggcctgg tggcgctgtt cggctcgctg
     1261 gcgcccaacg gcgcgatcct caaacgctcg gcggccgacc cgaggctgtt cgagaaggaa
     1321 gggcgcgcgg tggtgttcac ctcgctcgac gacctcgccg cgcgggtgga cgatccggcg
     1381 ctcgacgtga cgccggagga cttcatggtg ctgcagaacg cggggccgaa gtcgggctac
     1441 gccatgccgg aggccggcta cctgccgatc ccggcgaagc tcgcgcgcgc ggggctgaaa
     1501 gacatggtgc gcatctcgga cgcgaggatg agcggcaccg cctacggcac catcgtgctg
     1561 cacgtgtcgc ccgaggccgc cacgggcggc ccgctcgcgc tggtgcgcaa cggcgaccgc
     1621 atcaggcttt cggtgaagtc gcggcgcatc gatttgcagg tgcccggcga ggaactcgcc
     1681 aggcggcggg ccgcttggca gccgcccgtc gcgccgcccg cgcgcggcta cgccaagctc
     1741 tacatggacc acgtgctgca ggccgagcac ggctgcgatt tcgattttct gcgcaaggac
     1801 tgagccggcg gccatgagca gcgcgcgcag ccgtgagcac cgcaaggcgg cgacccgcgc
     1861 cggcctgcgc tacgtcaccg acggcttcgc cggcgtcacg cgcaagcgtt ccggcacggg
     1921 ctggacctat tacgcgcccg gcggcgcgcg catcgccgac gcggcgacgc gcaagcgcct
     1981 caacgcgctt gccatcccgc ccgcctggac cgacgtgtgg atctgccccg accccggcgg
     2041 gcacatccag gccacggcgc gcgacgcgcg cggcaggaag cagtaccgct accacccctc
     2101 ctaccgcgag gcgcgcgacc agtccaagtt ccgccgcatg ctcgaattca gcgaggcgct
     2161 gccgcagctg cgcgagcgcg tggagcgcga cctgcgcgcc gaggacctct cccgccggca
     2221 gctgctggcg acggtggtgc ggctgctgga ccgcacgctg atccgcgtcg gcaacgacga
     2281 gtatgcgcgc gagaaccagt ccttcggcct caccacgctg cgccggcgcc acgtgcaggt
     2341 ggagggcgac cggctgcgct tcgcgttccg cggcaagagc ggcgtcgagc attcgatctc
     2401 ggtcgccgac ccgcgcctgg cgcgcatcat ccagcgctgc caggacctcc ccgggcagga
     2461 gatgttccag tacctcggcg cggacaagaa gcgccagacc gtgtcctcgg acgacgtcaa
     2521 cggctacctg cgcgaggcga gcgatcgcga cgtcaccgcc aaggacttcc gtacctgggg
     2581 cggcaccatg ctcgcggcgg tggagctgcg cgccatgggt ccggcggcga gccggcgcga
     2641 ggccgatcgc aacatcctgc gcgcgctcga tgcggtggcc gagcgcctgg gcaacacgcg
     2701 caccgtgtgc cgcaagtact acgtgcatcc ggcgctgctg caggcctacc tcgtcggccg
     2761 caccgcgccc tggccgccgg cggcggacaa gcgcgccaag cggcgcgcca agccgggcgg
     2821 cgcgctgcgg cgcgacgagg tggcggtgct gcaattcctg caggaacgcg tgccggattg
     2881 agcggcgcct tccagctcgc cgacgtcggc gccgcgcagt acctgctgat cggcgcggtg
     2941 gccttcgcgg cctcgatcct cggcgggctg gcgggctacg gcacgggcct cgtggtgccg
     3001 atcttcctcg cgccggtggt ggggatcgcc aacgtggtgc cggtgatggc ggtgggcatg
     3061 atctttgcga acggcagccg cgtcgccgcg ttctggcgcg acatccaatg gccgcacgcg
     3121 cggcggattc tgcttttcgg cctgccggcc tgcgtggcgg gcgcctacgg ctacaccctg
     3181 ctggcggcga actgggtagc gctgctgctg ggcgtgtttt tcatcgccag cgtgccgttg
     3241 cgccgggccc tggtgcgcgc cggctggacg ctcgaaggga ggggcgtggc ggcggcgggc
     3301 gccggattcg ggttcgtgaa cggcggcgtc cccggcgcgg ggctgctcct catctccact
     3361 ctgatggcgg cgggactcgc cggccccgcg ctcatcgcca ccgacgccat cgtctcgatc
     3421 atcatgggcg cagcgaaaat cgcgctgttc ggcagcgtgg cgcgcctcga tgccgggctg
     3481 gcgctcgccg gcgtgctgat cggcctgtgc accgtgcccg gtgcgttcgt cgcgcggcgg
     3541 ctgctcgcgc gcatatcgct caaggtgcac actgtcgtga tggagatcgt ggtgctcgcc
     3601 ggcggcgccg ccttcctgtg gcgcgcagtc gcatgaagga cgaatcgcag gccatcggcg
     3661 gctatctcgc gcgcgtgccg gaggcgcggc gcgagcgcgc gctcgccctg cacggcctga
     3721 tcctgagcct gtttccgcag gccgaggtgt cgctcgagca gcgcatgccg acctaccggc
     3781 tcggggagga gtggatcgcc tgggcgagcc agaaggggca cctcgccctt tacacctgcg
     3841 ccgaggaccg cgtcgcgggg ttccgcgcgc gccaccccga ggtggacgcg ggcgagggct
     3901 gcctgcgctt tcgcgacagc cagcgcctgc ccctggcgga cctcgcccag gtggtgcgcc
     3961 gggcgctcgc gccggagctt gccaggcgcc ggcgctaggc agatacttta ggcttgagct
     4021 attcaagcct catggataga gaaacgcggg caacggtgga ggaccaccgc gcgctgcgcc
     4081 tgtggctgcg cctgctcacc tgctcgcagc tgatcgagcg ccaggtgcgc tcgcgcctcc
     4141 gggagcgttt cgacaccacg ctgccgcgct tcgacctcat gtcgcagctc gagcgccacc
     4201 cggcgggcct gaagatgaac gagctctcgc gcctgctgat ggtcaccggc ggcaacgtca
     4261 ccggcatcgt cgaccagctg gtgaaggaag gcctggtcga gcgcgtggac gacgccgcgg
     4321 accggcgcgc ctggcgcgta cggctcacgc gcgccggcga caaggccttc gccgagatgg
     4381 cgcgcgcgca cgaggaatgg gtggcggagc tgctttccgg cgtgtcgcgg cgcgacagcg
     4441 acgccctgat gcagctcctc gcccgcctga agcaggccac cctggagaaa tcggcatgat
     4501 ccgcacccaa cacttcctgt ggcgcgtcga cggcaaggtg ggcgtgctga cgctcaaccg
     4561 ccccgagaag aagaacccgc tcaccttcga gtcctacgcc gagctgcgcg cgctgttcca
     4621 ggggctgcag caggaaagcg aggtgaaggc cgtggtgttg acgggtgcgg gcggcaactt
     4681 ctgctccggc ggcgacgtgc acgagatcat cggtccgctg acgaaaatgc agccgccgca
     4741 gctcctcgag ttcacgcgca tgaccggcga cgtggtgaag gcgatgcgcg cctgcccgca
     4801 gcccatcgtc gccgcggtgg aaggcgtgtg cgccggcgcc ggcgcgatgc tggccctgtt
     4861 ctcggacctg cgctacggca agccggatgc gcagacggcg ttcctcttca cgcgcgtcgg
     4921 cctcgccggc gccgacatgg gcgcctgcgc catgctgccg cgggtgatcg ggcaggggcg
     4981 ggcggcggag ctgttgtact ccgggcgcgc gatgacggcc gaggaaggcc tcgcctgggg
     5041 cttcttcaac cgcctcgcgg atcccgtgct gccggcggcg atggagatcg cgcagaaact
     5101 ggcgtcgggg ccgactttcg cccatggggt gacgaagaag atgctgcacc aggaatggaa
     5161 cgcgggcctc gacgaggcga tcgaaatgga agcgcaggcg caggcgatct gcatgcagac
     5221 caaggacttc cagagggcct acgaggcgtt tgccgcgaag caaaagccca aattcgaggg
     5281 tgactagtgg ataggttctc gcatttgcag tggcctttcc tcgatccact gcacgcgacc
     5341 ctgtcgcgtg cagtggacgc ttgggcgctc gagaagctcg actacgcgca tggagaggac
     5401 gcggacgccg tctgccggag gctggtcaag gacctggggc aggctggtta cctgaaatac
     5461 tgtgtcaacg aaaatcccga cgtaaggtcg atagccttga tccgcgaggt actgtcgtac
     5521 cacgccggcc tcgccgactt ctccttcgtc atgcagggcc tgggcagcgg cccgatcgtc
     5581 ctggcgggca gcgacgcgca gaagcagaaa tacttgcccg cggcggcggc tggcgaggcg
     5641 atcgccgcct tcgcactctc ggagcccgac gccggatcgg acgtccaggc gatgaccgcc
     5701 actgcgcagc gcagcggtgc gcactgggtg ctggagggca ccaagacctg gatctccaac
     5761 ggcggcatcg ccgatttcta cgtcgccttc gcgaaggccg atgaaggcat cagcgcattt
     5821 gtcgtcgacg cgaaggaggt ggacgcctcc gagcgcatcg agatcgtcgc cccgcacccg
     5881 atggcgacgg tgagattcaa gggttcgaag ggcgagttgg tgggcgaggc ggggcagggc
     5941 ttcaagctgg cgatgcgcaa cctggacgtg ttccgcacca cggtggcggc ggcggcggtg
     6001 ggtttcgcgc gccgcgcctt cgacgaggcc ctcgcgcgcg tgaaacagcg ccgcatgttc
     6061 ggccagcgcc tcgccgacct gcagatgacg caggccaagc tcgccgacat ggccaccgcc
     6121 atcgacgccg cggcgctgct cacctaccgc gcggcctggc tgcgcgacgt gaaacggcag
     6181 cgcgtcacgc gcgaagcggc gatggccaag atgcaggcga cggaagccgc gcaggcggtg
     6241 atcgacgacg cggtgcagct gctcggcggc ctcggggtga tgcgcggcca cccggtcgag
     6301 cgcctgtacc gcgaggtgcg cgcgctgcgc atctacgaag gcgccaccga ggtgcagaag
     6361 gtgatcatcg cgcgggagct cctgaaatga agtggaagtc ggcgcacctg gacagcttcg
     6421 cgctcgacaa cctgccgccg cgcggccagt ggccggagtt cctcttcgag cggcccgagt
     6481 tccgctaccc gggccgcatg aactgcgcca ccgagctgct cgacaagatg gtgagccgcg
     6541 gcttcggcca ccgcccgctg atccgcacgc cggagggcgg ctgcacctac accgagctct
     6601 atgcgcaggc caaccgcatc gccaacgtgc tggtgcgcga catgggcctg cagccgggaa
     6661 accgggtgct gctgcgcggc cccaacagcc cgatgatggc ggcctgctgg ttcgcggtga
     6721 tgaaggcggg cgccgtctgc gtggcgacca tgccgctgct gcgcgccaag gagctgaccg
     6781 aaatcgtgca gaaagcgcag gtcggttttg ccctgtgcga ccggcgcctc gccgcggagc
     6841 tcgaggccgc gctgccggcc tgcccgacgc tgcagagcgt caagtactgg cacgacgacg
     6901 ccaaggattc tctcgatggg ctctgcgtgc gggcgcccca ggtgttcgcc aacgtggaca
     6961 ccgctgccga ggacgtggcg ctcatcgcct tcacctcggg caccaccggc aagccgaagg
     7021 gcacgatgca cttccaccgc gacgtggtgg cgatgtgcga ctgcttcccg cgct
//
LOCUS       DASRIE010000144         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1457220, whole genome shotgun sequence.
ACCESSION   DASRIE010000144 DASRIE010000000
VERSION     DASRIE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457220"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFB53_06070"
     CDS             complement(<1..496)
                     /locus_tag="VFB53_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86337.1"
                     /translation="MGAIFSSLGRTVVAGVVLLVIFIVLAGLGTGTWVRAGDHAWWTF
                     FMRWLHVLSGVMWIGLLWYFNFVQTPSMPKIPDEHKPAISKVIAPAALFWFRWGAMAT
                     IVTGLILATMQGYIGAALSLQKGYHAIGLGMWLGAIMWFNVWFIIWPNQKKALGMVTV
                     TPEEK"
     gene            570..1340
                     /gene="otnI"
                     /locus_tag="VFB53_06075"
     CDS             570..1340
                     /gene="otnI"
                     /locus_tag="VFB53_06075"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HZN86338.1"
                     /translation="MPKLAANLSTLFPQLDFLERFAAAARAGFRYVEYQFPYAWKPDE
                     LAKRAREAGVRVVLHNLPAGDFAKGERGIACLPGREAEFRDGVERGIAYAKAVDCPRL
                     NCLAGLAPADERHFAVLVENVRYAARRLGVAGLQLTLEAINTRTVPGFFLSRSAQAIE
                     VLNAAGEGNAFLQYDFFHMQLMEGDLAGTVERLLGRIGHIQLADVPGRHEPGTGELNF
                     DFLLRHLDALGYSGWVGCEYNPRGDTLEGLKWALPYLS"
     gene            1316..2773
                     /locus_tag="VFB53_06080"
     CDS             1316..2773
                     /locus_tag="VFB53_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN86339.1"
                     /translation="MGAALPVVAALREFLPQRAILHEEEDTRPYECDGLTAYRQLPMV
                     VALPETEEQVRRILQVCAALKVPVVPRGAGTGLSGGALPPGDGVLLSMAKFMRVLRID
                     PLARVAVVQPGVRNAVVSELAAPHGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGL
                     TLHNVLGVRGFTIDGEPIELGGEASDAPGYDLLALAIGSEGMLFVLTEVTLKLLPKPQ
                     CARVIMASFDDVEKAGGAVADIIGAGIVPAGLEMMDRPATRAVEQFVGAGYDLEAAAI
                     LLCESDGTPEEVDDEVARMKAVLERAGATRMTVSRDETERLRFWSGRKAAFPAVGRIS
                     PDYYCMDGTIPRKRLGEVLAFIGSLETRYGLRCPNVFHAGDGNLHPLILFDANNADEL
                     YRTELFAAEVLEKCVEVGGTITGEHGVGVEKINQMCVQFGAPELELFHGVKRAFDPHG
                     LLNPGKAVPSLARCAEYGRMRVHGGRLPHPELPRF"
     gene            2770..3816
                     /gene="glcE"
                     /locus_tag="VFB53_06085"
     CDS             2770..3816
                     /gene="glcE"
                     /locus_tag="VFB53_06085"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HZN86340.1"
                     /translation="MILEEFQQRIRAAVDRKAALRLRGGGTKDFYGGALRGEVLDTRG
                     YSGIVEYEPTELVVTARCGTPLEELERILEQNAQCLPFEPPHFGAGATLGGCVAAGLS
                     GPRRAAAGALRDFVLGAKLIDGRGQALAFGGRVMKNVAGYDVSRLLAGSLGTLGLIAE
                     VSLKLAPRPQAQATLRLEAPQARALELMNRWAGQPLPISATAWREGELAVRLSGSTGG
                     VRAAAAKIGGAVAADDLWPGLREQTDAFFAGPEPLWRLSVPSAAPPLELPGRQLIEWN
                     GALRWLKSPAAPAAVRAAARTVKGHATLFRAADKSAGCFDALDPVAARLHRQLKTTFD
                     PAGVFNPGRIYPEF"
     gene            complement(3813..4166)
                     /locus_tag="VFB53_06090"
     CDS             complement(3813..4166)
                     /locus_tag="VFB53_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86341.1"
                     /translation="MRWIIWLLVFAAIGVFWYAIWLWYRRWQDRERASEERFAALVAE
                     VKPPLAQTQAVEAPSTLGQQKLLFEAAAKAGEAGEPVLAIQLYARLLSRFPESAFGAQ
                     ARAAIEAQKSKLAKA"
     gene            4186..5370
                     /gene="glcF"
                     /locus_tag="VFB53_06095"
     CDS             4186..5370
                     /gene="glcF"
                     /locus_tag="VFB53_06095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HZN86342.1"
                     /translation="MQTQLADFIRDTPEGREAEAILRKCVHCGFCTATCPSYQVLGDD
                     LDSPRGRIYLIKRALEGAPVSGKTRLHLDRCLTCRACETTCPSGVRYGRLVDIGRAYV
                     EKQTRRTPWDRLRRAALAFALPRTRLFSAALRLGQLFNLAPKTSRARAWPAPRHARKM
                     IALRGCVQPALAPSINAAAARVLDRVGITLLEVPGAGCCGALRFHLNDQEAGRGDMRA
                     LIDAWWPLVAGREVEAIVATASGCGVTIKEYGHLLALERNYPGKAERISEMTRDLCEV
                     IRPADLPQGARRGRVAFQSPCSLQHGQQIRGKVEAILARLGYELVPVRDAHLCCGSAG
                     TYSLLQPEIAGELRARKLAALQEGGPQVIATANIGCLAHLQAGSAAPVRHWIELVDEA
                     LA"
     gene            5389..>5740
                     /locus_tag="VFB53_06100"
     CDS             5389..>5740
                     /locus_tag="VFB53_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN86343.1"
                     /translation="MAPKTVRVACPHDCPDTCAMLVTVEGGVATRIQGDPSMPFTDGT
                     LCTKVAHYLERTYAPDRLLYPQRRIGKKGEGKFRRITWDEALDEIAARLKALAAEDPE
                     TILPCSYAGTMGLAQ"
BASE COUNT          875 a         1993 c         1999 g          873 t
ORIGIN      
        1 ccttctcttc cggggtcacc gtgaccatgc cgagcgcctt cttctgattc ggccagatga
       61 tgaaccacac gttgaaccac atgatggcgc cgagccacat gcccaggccg atcgcgtggt
      121 agcccttctg caacgacagc gccgcgccga tgtagccctg catggtggcg aggatcaggc
      181 cggtgacgat ggtcgccatc gcgccccagc ggaaccagaa cagcgccgcc ggcgcgatca
      241 ccttgctgat cgccggctta tgctcgtccg ggatcttggg catcgacggt gtctgcacga
      301 agttgaagta ccagagcagg ccgatccaca tcacgccgga gagcacgtgc agccagcgca
      361 tgaagaacgt ccaccaggcg tggtcaccgg cgcgcaccca ggtacccgtg cccaagccgg
      421 ccagcacgat gaagatgacg aggaggacga cgcccgcgac cacggtgcgt ccgagggaac
      481 tgaagatggc acccattctt ttctcctgtt cgtatgatgg gtttgcagcg aaaaaagaat
      541 agcacttgta cgcgggttat gctggcagca tgccgaagct cgcggccaac ctgtcgacgc
      601 ttttcccaca gctcgatttc ctcgagcgct tcgccgccgc ggcgcgggcg gggtttcgct
      661 acgtcgaata ccagtttccc tacgcctgga agccggacga actggcaaaa cgcgcgcgcg
      721 aggctggggt gcgggtggtc ctgcacaacc tgccggccgg cgacttcgcc aagggcgaac
      781 gcggcatcgc ctgcctgccc gggcgcgagg cggagttccg cgacggcgtc gagcggggca
      841 tcgcgtacgc caaggcggtc gattgtccgc gcctcaactg cctcgccggc ctggcgccgg
      901 cggacgagcg gcacttcgcg gtgctggtgg agaacgtgcg ctacgccgcg cgccggctgg
      961 gcgtggcggg cctgcagctc acgctcgagg cgatcaacac gcgaaccgtg cccgggttct
     1021 tcctgtcgcg ctcggctcag gcaatcgagg tgctgaacgc cgccggcgaa ggcaacgcat
     1081 tcctgcagta cgacttcttc cacatgcagc tcatggaggg cgatctggcg ggaaccgtgg
     1141 agcggctgct cggccgcatc ggccacatcc agctcgccga tgtgccgggc cgccatgagc
     1201 cgggaacggg cgagctcaac ttcgacttcc tgctgcgcca cctcgacgcc ctcggatact
     1261 cgggctgggt gggttgcgaa tacaatcccc ggggtgacac gctggaaggc ctgaagtggg
     1321 cgctgcccta cctgtcgtag ccgctctgcg cgagttcctg ccgcagcgcg cgatcctcca
     1381 cgaagaagaa gacacccgtc cgtacgagtg cgacgggctc accgcctacc gccagctgcc
     1441 gatggtggtg gcgctgcccg agaccgagga gcaggtaagg cgcatcctgc aggtctgcgc
     1501 cgcgctgaaa gtcccggtcg tgccccgcgg cgccggaacc ggcctctccg gcggcgcgct
     1561 gccgccgggc gacggcgtgc tcctctcgat ggcgaagttc atgcgcgtgc tgcgcatcga
     1621 cccgctggcg cgcgtcgccg tggtgcagcc gggcgtgcgc aatgcggtgg tttccgagct
     1681 cgccgcgccg cacgggcttt actacgcgcc ggatccctcg agccagatcg cctgcaccat
     1741 cggcggcaac gtgtcggaga actccggcgg cgtgcattgc ctcaagtacg gcctcacgct
     1801 gcacaacgtg ctcggcgtgc gcggcttcac catcgacggg gagccgatcg agctcggcgg
     1861 cgaagcgtcg gacgcgccgg gatacgacct gctcgcgctc gccatcggct ccgagggcat
     1921 gctgttcgtg ctcaccgagg tgacgctgaa gctgctgccg aagccgcagt gcgcgcgcgt
     1981 catcatggcc tcgttcgacg acgtggagaa ggcgggcggc gcggtcgccg acatcatcgg
     2041 cgccggcatc gtgcccgccg ggctggagat gatggaccgg ccggcgacgc gcgcggtgga
     2101 gcagttcgtc ggcgcgggct acgacctcga ggcggcggcg atcctgctct gcgagtcgga
     2161 cggcacgccg gaagaagtgg acgacgaggt cgcgcgcatg aaggcggtgc tcgaacgcgc
     2221 cggggcgacc cgcatgaccg tctcgcgcga cgagacggag cggctgcgtt tctggtcggg
     2281 ccgcaaggcc gcgtttcctg cggtggggcg catctcgccc gactactact gcatggacgg
     2341 caccattccg cgcaagcgcc tcggcgaggt gctcgcattc atcggttcgt tggagaccag
     2401 atacggcttg cgctgcccca acgtattcca cgccggcgac ggcaacctgc accccctgat
     2461 cctgttcgac gccaacaacg cggacgagct ctatcgcacg gagctgttcg cggccgaggt
     2521 gctggaaaag tgcgtcgagg tgggcggcac gatcaccggc gagcacggcg tcggcgtcga
     2581 gaagatcaac cagatgtgcg tgcagttcgg cgcgcccgag ctcgagctgt tccacggcgt
     2641 gaagcgcgct ttcgatccgc atggcctgct caatcccggc aaggcggtgc cgagcctcgc
     2701 gcgctgcgcg gaatacgggc gcatgcgggt gcacggcggc cgcctgccgc accccgagct
     2761 gccgcggttt tgatcctgga agaatttcag cagcgcatcc gggccgccgt ggaccggaag
     2821 gctgcgctgc gcctgcgcgg cggcggcacc aaggacttct acggcggcgc gctgcgcggc
     2881 gaggtgctgg acacgcgcgg ctatagcggc atcgtggagt acgaacccac cgagctggtg
     2941 gtcacggcgc gctgcggcac gccgctcgag gagctggaga ggatcctcga acaaaatgcg
     3001 cagtgcctgc ctttcgagcc gccgcatttc ggcgccgggg cgacgctcgg cggttgcgtc
     3061 gcggcggggt tgtccgggcc gcgtcgcgcc gccgcgggcg cgctgcgtga tttcgtgctc
     3121 ggcgcgaagt taatcgacgg gcgcggccag gcgctcgcgt tcggcgggcg cgtgatgaag
     3181 aacgtcgccg gctacgacgt ctcgcgcctg ctcgccgggt cgctcggcac gctcggcttg
     3241 atcgccgagg tatcgctcaa gctcgcgccg cgcccgcagg cgcaagccac gctgcgcctc
     3301 gaagcgccgc aggcgcgcgc cctggagctc atgaatcgct gggcggggca accgcttccg
     3361 atcagcgcca cggcgtggcg ggagggggag cttgcggtgc gcctttccgg ctcgacggga
     3421 ggcgtgcgcg ccgcggcggc gaagatcggc ggcgcggtcg ccgccgacga cttgtggccc
     3481 ggcttgcgcg agcagacgga cgcgttcttc gccgggccgg agccgttgtg gcggctttcc
     3541 gtgccctcgg ccgcgccgcc gctcgagctg ccgggacgcc agctcattga atggaacggc
     3601 gcgctgcgct ggctgaaaag ccccgcggcg ccggccgcgg tgcgcgccgc cgcgcgaacc
     3661 gttaaaggcc atgcgacgct tttccgcgcc gccgacaagt cggccggctg cttcgatgcg
     3721 ctcgacccgg tggcggcgcg cctgcatcga caattgaaaa ccacgttcga tcccgcgggg
     3781 gtattcaatc ccggccggat ctacccggag ttctaggcct tggcgagctt cgacttctgc
     3841 gcttcgatcg cggcgcgcgc ctgcgcgccg aaagcgcttt ccgggaagcg cgacagcagc
     3901 cgggcgtaca gctggatcgc cagcaccggc tcgcctgctt cgccggcttt cgccgcggct
     3961 tcgaacagca gcttctgctg cccgagcgtc gaaggagctt cgaccgcctg ggtttgggca
     4021 agcggtggtt tcacctcggc caccagcgcg gcgaagcgtt cttccgaagc gcgctcgcgg
     4081 tcctgccagc gccggtacca gagccagatc gcgtaccaaa atacgccgat cgctgcgaat
     4141 accagcagcc agatgatcca cctcatgcga aaagtatagc cgccggtgca gacccagctc
     4201 gccgacttca tccgcgatac gcccgagggc cgcgaagccg aagccatcct gcgcaagtgc
     4261 gtgcattgcg gcttctgcac cgccacctgc ccgagctacc aggtgctggg cgacgacctc
     4321 gacagcccgc gcgggcgcat ctacctgatc aagcgcgcgc tcgagggcgc gccggtgagc
     4381 ggcaagacgc gcctgcatct cgaccgctgc cttacgtgcc gcgcgtgcga gaccacctgc
     4441 ccttcgggcg tgcgctacgg ccgcctggtc gacatcggcc gggcgtacgt ggaaaagcaa
     4501 acgcggcgca cgccttggga ccggctgcgc cgggcggcgc tcgccttcgc gcttccccgc
     4561 actcggctgt tcagcgccgc gctgcgcctt ggccagcttt tcaatctggc gccgaaaacc
     4621 tcgcgggcgc gcgcatggcc ggcgccgcgg cacgcccgca agatgatcgc gctgcgcggc
     4681 tgcgtgcagc cggcgctcgc gccttcgatc aacgcggccg cggcgcgggt cctcgaccgc
     4741 gtcgggatca cgctcctcga agtgcccggc gcggggtgct gcggcgcgct gcgtttccac
     4801 ctcaacgacc aggaagccgg ccgcggcgac atgcgggcgc tgatcgacgc atggtggccg
     4861 ctcgtcgccg ggcgcgaagt cgaggccatc gtggcaacgg ccagcggctg cggcgtcacg
     4921 atcaaggaat acggccacct gctcgcgctc gagcgcaatt acccgggcaa ggccgagcgc
     4981 atttccgaga tgacgcgcga cctgtgcgag gtcatccggc ccgcggacct gccgcagggc
     5041 gcgcgccgcg ggcgcgtggc tttccagtcg ccgtgctcgc tacagcacgg ccagcagatc
     5101 cgcggcaagg tcgaagcgat cctcgcgcgg ctcggctacg agctcgtccc ggtgcgcgac
     5161 gcgcatctct gctgcggctc ggccgggacc tattcgctgc tccagcccga gatcgcgggc
     5221 gagctgcgcg ctcgcaagct cgccgcactg caggaaggcg ggccgcaggt catcgccacg
     5281 gcgaacatcg gctgcctggc gcatctgcag gccggcagcg ccgcgccggt gcgtcactgg
     5341 atcgaactcg tcgacgaggc gctggcgtag aatccccgca tgggtgacgt ggctccgaag
     5401 acggttcgtg tggcctgccc gcacgactgc cctgacacct gcgcgatgct ggtcacggtg
     5461 gagggcggcg tcgccacccg catccagggc gatccgtcga tgccgttcac cgacggcacg
     5521 ctctgcacca aggtcgccca ttacctcgag cgcacgtacg cccccgatcg cctgctctat
     5581 ccgcagagaa gaatcgggaa gaaaggcgaa ggcaagttcc gccgcattac ctgggacgag
     5641 gcgctcgacg agatcgcggc gcggctgaag gcgctcgcgg ccgaggaccc cgagaccatc
     5701 ctgccgtgca gctacgccgg caccatgggc ctcgcgcaat
//
LOCUS       DASRIE010000145         5996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1204529, whole genome shotgun sequence.
ACCESSION   DASRIE010000145 DASRIE010000000
VERSION     DASRIE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204529"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFB53_06105"
     CDS             complement(<1..923)
                     /locus_tag="VFB53_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZN86344.1"
                     /translation="MSKKRRTESRGRRARPAPPHRGAPPPALGKYTGRIEGHADGHGF
                     LAPDGGAARVYLAAAEMRQVMHGDRAMVRVTGIDHRERPTGEILEVLERVNRRIVGRL
                     HSDHGVLFLVPEDRRIAQDILVPPAEAGRAKPGQVVSVELVAQPSRHSQPVGRVAEVL
                     GNYADPGMEIEIALRKFDLPREFSRRAEAQARELPETVREEDARGRRDLRALNFVTID
                     GETARDFDDAVYAVREGKGFRLWVAIADVSHYVRDGDALDQDARARGTSVYFPRRVLP
                     MLPEKLSNGLCSLNPLVDRLAMVCEMAISGAG"
     gene            957..1043
                     /locus_tag="VFB53_06110"
     tRNA            957..1043
                     /locus_tag="VFB53_06110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:991..993,aa:Leu,seq:cag)
     gene            complement(1049..2341)
                     /locus_tag="VFB53_06115"
     CDS             complement(1049..2341)
                     /locus_tag="VFB53_06115"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZN86345.1"
                     /translation="MSRARNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGH
                     TLVIGAKKTVLHLIPSGILRGSVDCFIGNGVVVSPEALLAEMDELEHAGVSNVVARLI
                     ISEACPLILPYHVALDRAREAAKKIGTTGRGIGPAYEDKVARRALRLQDLLHPERFAA
                     KLEALLEFHNFVLQNYYRQPAVDYRRTLEETLALAPRLAPLVADVPRALYHANKAGKN
                     LLFEGAQGALLDIDHGTYPYVTSSNCVAGAAAAGAGIGPMHLHYVLGITKAYSTRVGE
                     GPFPTELSDDVGERLRQRGREFGATTGRPRRTGWFDAAALKRSIQLNGVSGLCITKLD
                     VLDGADALKICVGYRIGGQLSEILPVGAEELERCEPVYEELPGWNESTVGIKSHAGLP
                     QAARDYLKRIEDLAGVPIDLISTGPERDETIVLRHPFA"
     gene            complement(2371..3309)
                     /locus_tag="VFB53_06120"
                     /pseudo
     CDS             complement(2371..3309)
                     /locus_tag="VFB53_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
     gene            complement(3306..3491)
                     /locus_tag="VFB53_06125"
     CDS             complement(3306..3491)
                     /locus_tag="VFB53_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HZN86346.1"
                     /translation="MASTFLLAIALMLIIEGLLPFLAPSFWRDTFRRITQLTDGQIRF
                     FGLTSMLIGLLLLLLAR"
     gene            complement(3498..4382)
                     /gene="hflC"
                     /locus_tag="VFB53_06130"
     CDS             complement(3498..4382)
                     /gene="hflC"
                     /locus_tag="VFB53_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZN86347.1"
                     /translation="MRALGPLAAVLIALALVASSAVFTVDQRQNAIVFQLGEIKDVIT
                     RPGLHFKWPLIQNVRLFDMRILTFDDPEPLRFPTSGNKPLDVDSFVKWRITDVRLYFV
                     SVQGDEFRATTRLKQTISDGLQAEFGQRTVHDVVSGQRDDIMEAVRIEADKDLRRIGV
                     EIVDVRLKRVDLPTEVSASVYRRMESERKRVANESRSIGVAEGEKIRADADRQREVIL
                     ANAYRDAQKTKGEGDARSAAIYAQAFSQNAEFYSFYRSMEAYRSTFRGRNDLMVLEPS
                     SDFFRYLKDPSGKSGGRK"
     gene            complement(4379..5584)
                     /gene="hflK"
                     /locus_tag="VFB53_06135"
     CDS             complement(4379..5584)
                     /gene="hflK"
                     /locus_tag="VFB53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZN86348.1"
                     /translation="MSLNDPNWGRQGGNRGPGGNQGPPDLDELWRNFNRKLNELFGRR
                     GGGGEPPRVPPSGRALGGGAGLVAALVLGVWLASGFYIVDAASRGVVLTFGKFAGETD
                     PGLRWRMPYPLQSHEIVNLSQVRTVEVGYRNNNVKMRVDKEALMLTDDENIVSVQFAV
                     QYLIKDAQVYLFVNKRPDETVQHVAETAMREVIGKSKMDAILYESRASINASAKKLMQ
                     QILDRYQTGVSVSTVTIQSAQPPQDVQAAFSDQQSANQDRERHKNEGEAYANDVIPKA
                     RGTASRLKEEAEGYKQRVIANAEGEASRFRQVLAEYAKAPAVTRERIYLETMQQVLSS
                     TSKIVVDTKGSGNLLYLPLDKLMQQGGAASLSEAQASQRTAPEPAAAPPEAGARSRDS
                     LRGRERGER"
     gene            complement(5595..>5996)
                     /locus_tag="VFB53_06140"
     CDS             complement(5595..>5996)
                     /locus_tag="VFB53_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN86349.1"
                     /translation="EAGNVVVSDTVGFIRDLPHGLVAAFRATLEETVHADLLLHLVDS
                     ASPVREQQAAEVDKVLQEIGAGDVPQLLVWNKADLSGLAPGAERDEYGRIRRVFVSAR
                     TGAGLAELRGAIAEAAQTRPARSPSPELISL"
BASE COUNT          833 a         2119 c         2118 g          926 t
ORIGIN      
        1 ccggcgccgc tgatcgccat ctcgcagacc atcgccaggc ggtccaccag cgggttgagc
       61 gagcacaggc cgttggagag cttctccggc agcatgggca gcacgcggcg cgggaagtac
      121 accgaggtgc cgcgcgcgcg cgcatcctgg tcgagcgcat cgccgtcgcg cacgtagtgg
      181 ctcacgtcgg cgatcgccac ccacagacgg aaacccttgc cctcgcgcac ggcgtagacc
      241 gcgtcgtcga aatcccgcgc cgtctcgccg tcgatggtga cgaaattgag cgcgcgcagg
      301 tcgcgccggc ccctcgcgtc ctcctcgcgc accgtttccg gcagctcgcg cgcctgcgcc
      361 tcggcgcgcc gcgagaactc gcgcggcagg tcgaacttgc gcagcgcgat ctcgatttcc
      421 atgcccgggt cggcgtaatt gcccagcacc tcggcgacgc ggccgacggg ctgcgagtgc
      481 ctggagggct gcgccaccag ctcgacgctc accacctgcc ccggcttggc gcgcccggct
      541 tcggccggcg gcaccaggat gtcctgcgcg atgcgccggt cctcgggcac caggaacagc
      601 acgccgtgat cgctgtgcag gcggccgacg atgcgccggt tgacgcgctc gaggacctcg
      661 aggatctcgc cggtggggcg ctcgcggtgg tcgatgccgg tgacgcgcac catcgcgcgg
      721 tcgccgtgca tcacctggcg catttcggca gccgcgaggt acacgcgcgc cgcgccgccg
      781 tcgggcgcga ggaagccgtg gccgtcggcg tggccttcga tgcgcccggt gtacttgccg
      841 agcgcgggcg gcggcgcgcc gcggtggggc ggcgccggtc tggcgcggcg gccgcgcgat
      901 tcagtgcgac gtttctttga cacgcatttc ctcagacact agaattcgcc tcccctgccg
      961 aggtggcgga attggtagac gcactaggtt caggtcctag cggtggcaac actgtggggg
     1021 ttcgaatccc ttcctcggca ccacacactc aggcaaacgg atggcgcagg acgatggtct
     1081 cgtcgcgctc cgggccggtt gaaatcaggt cgatcggcac tcccgccagg tcctcgatgc
     1141 gcttgagata gtcgcgcgcg gcctgcggca gcccggcgtg ggacttgatg ccgacggtgc
     1201 tttcgttcca gccgggaagc tcctcgtaga ccggctcgca gcgctcgagt tcctcggcgc
     1261 ccaccggcag gatctcggag agctgccccc cgatgcggta gccgacgcag atcttgagcg
     1321 catcggcgcc gtccagcacg tcgagcttgg tgatgcacag tcccgacacg ccgttcagct
     1381 ggatcgagcg cttgagcgcc gccgcgtcga accagccggt gcgccgcggc cggccggtgg
     1441 tggcgccgaa ctcgcggccg cgctggcgca gccgctcccc gacatcgtcc gacagctcgg
     1501 tcgggaacgg cccttcgccc acgcgcgtgc tgtaggcctt ggtgatgccc agcacgtagt
     1561 gcaggtgcat cgggccgatg cccgcgcccg cggcggccgc gcccgccacg cagttggacg
     1621 aggtgacgta gggataggtg ccgtggtcga tgtcgagcag cgcgccctgc gcgccctcga
     1681 acagcaggtt cttgccggcc ttgttggcgt ggtacagcgc gcgcggcacg tcggccacca
     1741 gcggcgccag gcgcggcgcg agcgcaagcg tctcctccag cgtcctgcgg tagtcgaccg
     1801 ccggctggcg gtagtagttc tgcagcacga agttgtggaa ctcgagcagc gcttcgagct
     1861 tggcggcgaa gcgctccggg tgcagcaggt cctgcagccg cagcgcgcgc cgcgccacct
     1921 tgtcctcgta cgcggggccg atgccgcggc cggtggtgcc gatcttcttc gccgcttcgc
     1981 gcgcgcggtc gagggcgaca tggtagggca ggatcagcgg gcaggcttcc gagatgatca
     2041 ggcgcgcgac gacgttgctc accccggcgt gctcgagctc gtccatctcg gcaagcaggg
     2101 cttcgggcga caccaccacg ccgttgccga tgaagcagtc gacgctgccg cgcaggatgc
     2161 ccgaggggat caggtgcagc acggtcttct tcgcgccgat caccagcgtg tggccggcgt
     2221 tgtgcccgcc ctggaagcgc accacgccct gcgcgcggtc ggtgagccag tcgacgatct
     2281 tgcccttgcc ctcgtcgccc cactgcgtgc cgatgacgac gacgttgcgc gcccggctca
     2341 cgcgcgcttc cccttggcga gcgcggccga agcgaggctg cgcaagtcga tggaaaagcc
     2401 ggtcgccggc cgcgcgcggc cgaaggcctt gccgacctcg tcgtagcgcc cgccgcggcc
     2461 gaccgcgccg gccacgccgt cgcagtaggc gtcgaacacc acgcccgaat ggtagtggta
     2521 gccgcgcagc tcggcgaggt cgaagctcgc cgggatcggg caggccctgg ccagcgcgcg
     2581 cagcgtggcg agcgcgcgcc gcaactccgg gatggcgggc agcctgcgcc gcgccagcgc
     2641 gagcacctcg gcgccgccgt agagctcggg cagcagcagc agcgcgccgc gcgtcttggc
     2701 ggggaggccg cgcgtcaggc ccttgagcgc cggcaggtcc ttgcgctgca gcgcctcgaa
     2761 cagctcggcc tcgggcgcgg cggcgacccc ggcggcgcgc accagcgagc ggaacaccgc
     2821 cacgtggccg atgtccatgc gcggcttctt cacgcgcgcc agcgccagcg cgcggcacag
     2881 cagccgctgc acctccaggt cgctccccac cccggcgtgg ccgtagatct cggcgccgat
     2941 ctggatcggc tcgcgcgtcg ccgccggccc ggcgggccgc gtgtgcagca cgctgccgca
     3001 gtagcacagg cgcgtcaccc ccttgcggtt cagcaggtgc gcgtcgatcc tcgccacctg
     3061 gggcgtgatg tcggcacgca ggcccatcat gcggccggag agctggtcca ccaccttgaa
     3121 ggtgcgcacg tcgaggtcgt gcccggtccc ggtgagcagc gaatccatgt attcgagcag
     3181 cggcggcatc accagctggt agccggcgcc ggcgaacagg tcgagcagcg cgcggcgcag
     3241 ccgctcgatg cgcgccgcct cggcgggcag gatgtcctcg atgtactcgg gcaggagcca
     3301 gcgcatcagc gtgccagcag cagcagcagc aggccgatca gcatcgaggt gaggccgaag
     3361 aagcggatct ggccgtcggt gagctgcgtg atgcggcgga aggtgtcgcg ccagaagcta
     3421 ggcgcaagga aggggagcag cccctcgatg atcagcatca gggcgatggc caggaggaag
     3481 gtgctcgcca tggcccatca tttcctgccg ccggatttgc cggagggatc cttgaggtag
     3541 cggaagaagt ccgagctcgg ctcgagcacc atcaggtcgt tgcggccgcg gaacgtcgag
     3601 cggtaggcct ccatgctgcg gtagaaggaa tagaactcgg cgttctgcga gaacgcctgc
     3661 gcgtagatcg ccgcggatct ggcgtcgccc tcgcccttgg tcttctgcgc gtcgcggtag
     3721 gcgttggcca ggatcacctc gcgctggcgg tcggcgtcgg cgcggatctt ctcgccctcg
     3781 gccacgccga tggagcgcga ctcgttggcc acgcgcttgc gctcggattc catgcgccgg
     3841 tacaccgagg cgctcacctc ggtgggcagg tcgacgcgct tcaggcgcac gtcgacgatc
     3901 tccacgccga tgcgccgcag atccttgtcg gcctcgatgc gcaccgcctc catgatgtcg
     3961 tcgcgctgcc cggacaccac gtcgtgcacc gtgcgctggc cgaactcggc ctgcaggccg
     4021 tcggagatgg tctgcttcag gcgcgtggtg gcgcggaact cgtcgccctg caccgacacg
     4081 aagtacagcc gcacgtcggt gatgcgccac ttgacgaagg agtccacgtc gagcggcttg
     4141 ttgcccgaag tggggaagcg cagcggctcc ggatcgtcga aggtgaggat gcgcatgtcg
     4201 aacaggcgca cgttctggat cagcggccac ttgaagtgca ggccgggcct ggtgatcacg
     4261 tccttgatct cgcccagctg gaacacgatg gcgttctgcc gctggtcgac ggtgaacacc
     4321 gcgctcgagg cgaccagcgc cagcgcgatg aggaccgcgg cgagcgggcc gagggccctc
     4381 atcgctcgcc cctttcgcgg ccgcgcaggc tgtcgcgcga gcgcgcgccg gcctcgggcg
     4441 gcgccgcggc gggctcgggc gccgtgcgct gcgaagcctg cgcttccgac agggaagccg
     4501 cgccgccctg ctgcatcagc ttgtccagcg gcaggtacag caggttgccg ctgcccttgg
     4561 tgtcgaccac gatcttcgag gtcgaggaca gcacctgctg catggtctcg aggtagatgc
     4621 gctcgcgcgt gacggccggc gccttggcgt actcggccag cacctggcgg aagcgcgagg
     4681 cctcgccctc ggcgttggcg atcacgcgct gcttgtagcc ctcggcctcc tccttcaggc
     4741 gcgaggcggt gccgcgcgcc ttcgggatca cgtcgttggc gtaggcctcg ccctcgttct
     4801 tgtggcgctc acggtcctgg ttggcgctct gctggtcgct gaacgccgcc tgcacgtcct
     4861 gcggcggctg cgccgactgg atggtcaccg tggacaccga caccccggtc tggtagcggt
     4921 cgaggatctg ctgcatcagc ttcttggcgc tggcgttgat gctggcgcgc gactcgtaca
     4981 ggatggcgtc catcttgctc ttgccgatca cctcgcgcat cgcggtttcc gccacgtgct
     5041 gcaccgtctc gtccgggcgc ttgttgacga acaggtacac ctgcgcgtcc ttgatcaggt
     5101 actgcacggc gaactgcacg ctgacgatgt tctcgtcgtc ggtgagcatc agcgcttcct
     5161 tgtccacgcg catcttcacg ttgttgttgc ggtagccgac ctccaccgtg cgcacctgcg
     5221 acaggttgac gatctcgtgc gactgcagcg gatagggcat gcgccagcgc aggccgggat
     5281 cggtctcgcc ggcgaacttg ccgaaggtca gcaccacgcc gcgcgaggcg gcgtcgacga
     5341 tgtagaaccc gctcgccagc cacacgccca gcaccagcgc cgccaccagg ccggcgccgc
     5401 cgcccagcgc gcggcccgac ggcggcacgc gcggcggctc gccgccgccg ccgcgccggc
     5461 cgaacagctc gttcagcttg cggttgaagt tgcgccacaa ctcgtcgagg tcgggcgggc
     5521 cctggttgcc gccggggccg cggttgccgc cttggcgccc ccagttcggg tcgttcagag
     5581 acatggatgc tcgactacag agaaatcagt tcaggagaag ggctgcgcgc cggccgcgtc
     5641 tgcgcggcct cggcgatcgc gccccgcagt tcggcgaggc cggcgccggt gcgcgcgctc
     5701 acaaatacgc ggcgaatcct accatactcg tcgcgctcgg cccccggcgc gaggccggag
     5761 aggtccgcct tgttccagac caggagctgc ggcacgtcgc cggcgccgat ctcctgcagc
     5821 accttgtcca cctcggcggc ctgctgctcg cgcaccggcg aagcgctgtc caccaggtgc
     5881 agcagcaggt cggcgtgcac ggtctcctcc agcgtggcgc ggaacgccgc caccaggccg
     5941 tgcggcaggt cgcggatgaa gccgaccgtg tcggatacca cgacgtttcc agcctc
//
LOCUS       DASRIE010000146         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_931045, whole genome shotgun sequence.
ACCESSION   DASRIE010000146 DASRIE010000000
VERSION     DASRIE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931045"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VFB53_06145"
     CDS             complement(<1..1132)
                     /locus_tag="VFB53_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86350.1"
                     /translation="MTLTDSRGNAVTAGSRTALALCEQAIDQFAGFAGDPFAKLDAAL
                     AENPDFIMPHLVRGGILASSTERGAEPLLRECVQAASALSQHANERERAHLAALRAWL
                     DRDFDRSVALYGRLLIDHPRDLFALQMAHLGDFYLGQTGLQRDRVARALPDWDAAVPG
                     FGFVLGMYAFGLEENAHYTAAEETGRRAVELNPRDAWAIHAVAHVMEMQGRQQEGITW
                     MTGNTEGWSRDSFFAVHNWWHVALYHLDLGQTDEALKLFDGPIYGAKSRITLEMIDAS
                     AMLWRMHLRGVDVGDRWQAVADAWEPIKGAGNYAFNDVHAAMAFVGAGRRDSLARVID
                     AQKEAMQRVGDNAMFTRKVGHPVVLAIKAFGDGNYAEALWRLR"
     gene            1326..>3093
                     /locus_tag="VFB53_06150"
     CDS             1326..>3093
                     /locus_tag="VFB53_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN86351.1"
                     /translation="MPHARLAILGGFQLFSMQGDPVALTARKPRALLAYLALSPGRPH
                     ARERLATLLWGDSSDQQARTSLRQALTAVRRGLPEADSVISADTDCVALAPSALEVDA
                     LVFEQAVGLGTVEALQSGLQLYRGDLLDGFSADAPAFEQWLVAERERLRGLAVQALSA
                     LFEFQRRLGLSDAAVETAARLVALDPLREDTQRDLIRLYLQQGRMVEALRQYQSVRQV
                     LARELGVQPAPETEQLYREIVQQRRTVTVAEEKAAPAPPEPPRAAPAVRHAIVMLADL
                     HRFTTFAGEIDPELVHNYLVAYRRRVEDVVQSHGGIITNYIGARVMVAFGVPVAHDNE
                     AERAVRAALALRDAIPQLVAPGGWRLNAQIGLASGSVFVDTAGAGPLVISGEPVSMAA
                     RIMEQAQPSEVLAAGWVRHALGERLRAEALATVTVRGAARPVQVWRLAEWLERVPRMH
                     PFVGRQLELRQIAGLLEGCLVSRTAQVLLVRGEAGIGKTRLLEELNRMARARGFVGYR
                     AAVLDFGVGSGGGAIASLVRGMLNVHERDSPDNLRAAAERAIEAGEVDRERAAFLYGM
                     LELDVPQPLDRTAVDAGARQRAE"
BASE COUNT          432 a         1058 c         1124 g          479 t
ORIGIN      
        1 cgcgcagccg ccagagcgct tccgcgtagt tgccgtcgcc gaacgccttg atcgccagca
       61 cgaccgggtg gccgaccttg cgcgtgaaca tggcgttgtc gccgacgcgc tgcatcgcct
      121 ccttctgcgc gtcgatcaca cgcgcgagcg agtccctgcg cccggcaccg acaaaggcca
      181 ttgccgcgtg cacgtcgttg aacgcgtagt tgccggcgcc cttgatcggt tcccaggcat
      241 ccgccaccgc ctgccagcgg tcgccgacgt cgacgccgcg cagatgcatc cgccacagca
      301 tcgccgaggc gtcgatcatc tccagggtga tgcgcgactt ggcgccgtag atcggcccgt
      361 cgaacagctt cagcgcctcg tcggtctggc cgagatcgag atgatagagt gcgacatgcc
      421 accagttgtg gaccgcgaag aagctgtcgc gcgaccatcc ctcggtgttg ccggtcatcc
      481 aggtgatgcc ctcctgctgg cggccctgca tctccattac gtgcgcgacg gcgtgaatcg
      541 cccaggcgtc gcgcggattg agctccaccg cgcgccgtcc cgtctcctcg gcggcagtgt
      601 agtgggcgtt ctcctccagg ccgaaggcat acataccgag cacgaagccg aaccccggca
      661 ctgctgcgtc ccagtcgggc agcgcgcgcg ccacgcggtc gcgctgcagg ccggtctgcc
      721 ccaggtagaa atcgcccagg tgcgccatct gcagggcgaa caggtcgcgc gggtggtcga
      781 tcagcaggcg tccgtagagg gcgacgctgc ggtcgaaatc gcggtcgagc cacgcgcgca
      841 gcgcggccag gtgagcgcgt tcgcgctcgt tggcgtgctg gctcagcgcc gaggcggcct
      901 gcacacactc gcgcagcagc ggctcggcgc cgcgttcggt gctgctggcg aggatgccgc
      961 cgcgcaccag gtgcggcatg atgaaatcgg ggttctcggc gagcgccgca tccagcttgg
     1021 cgaaggggtc gccggcaaag cccgcgaact ggtcgatcgc ctgctcgcag agcgcgagtg
     1081 ccgtgcggct gccggcggtt accgcatttc cgcgtgagtc ggtgagcgtc atggtgcttc
     1141 cttgcgaggt ggatgtggtg cgacggatgc catcttgcgg tcatggcgtg aggcgatcgt
     1201 tgcgcgcgcc gtgaaaacgg cgtgaatccg cgccggctgt gaacggcttc acagcgcgca
     1261 tacgcgtcgc gcatagtgtc ggagctatgg cggtagaatc accatccgct ctggccgata
     1321 cccgcatgcc gcacgcgcgc ctcgcaatcc ttggtggctt tcagctgttc tcgatgcagg
     1381 gagacccggt cgcgctcacc gcgcgcaagc cgcgcgcgct gctggcctac ctcgccctgt
     1441 cgcccggcag gccccacgca cgcgagcggc tggcgaccct gctgtggggc gacagctcgg
     1501 accagcaggc gcgcaccagc ctgcgccagg ccctcaccgc ggtgcggcgc gggctgcccg
     1561 aggccgattc tgtaatttct gccgatacgg attgcgtcgc gctggcaccg agcgcgctgg
     1621 aggtggacgc gctcgtcttc gagcaggcgg tcgggctggg taccgtcgag gcactgcaga
     1681 gcgggctcca gctctatcgg ggcgacctgc tcgacgggtt cagtgccgac gcacccgcgt
     1741 tcgagcaatg gctggtggcc gagcgcgagc gcctgcgcgg cctggccgtg caggcgctgt
     1801 cagccctgtt cgagttccag cgccggctgg gtctgagcga tgccgcggtc gaaaccgcgg
     1861 cgcgcctggt ggccctcgat ccgctgcgcg aggacacgca gcgcgacctg atccggctgt
     1921 acctgcagca gggccgcatg gtcgaggcgc tgcgccagta ccagtcggtg cgccaggtgc
     1981 tggcgcgcga gctgggcgtg cagccggcgc cggagaccga gcagctctat cgcgagatcg
     2041 tgcagcagcg ccgcaccgtg acggttgccg aggagaaggc agcgccggct cctcccgaac
     2101 ccccgcgggc ggcaccggcg gtgcggcatg caatcgtgat gctggccgac ctgcaccggt
     2161 tcaccacctt cgccggcgag atcgacccgg agctggtgca caactacctc gtggcgtacc
     2221 ggcggcgtgt cgaggacgtg gtgcaatcgc acggtggcat catcaccaac tacatcggcg
     2281 cccgcgtgat ggtggccttc ggcgtgccgg tggcgcacga caacgaagcc gagcgcgcgg
     2341 tgcgcgccgc gctcgcgttg cgcgacgcga tcccgcagct ggtggcgccc ggcggatggc
     2401 ggctgaacgc acagatcggg cttgccagcg gatcggtctt cgtcgacacc gcaggcgcag
     2461 gaccgctggt gatctcaggc gagccggtca gcatggcggc acgcatcatg gagcaggcgc
     2521 agcccagcga ggtcctggcg gcggggtggg tgcgccacgc gctgggcgag cggctgcgcg
     2581 ccgaggcgct tgccacggtc acggtgcgcg gagctgcgcg accggtgcag gtctggcgcc
     2641 tggcggaatg gctggagcgc gtgccgcgca tgcatccctt cgtgggccgc cagctcgagc
     2701 tgcgccagat cgcgggcctg ctggagggtt gcctggtttc gcgaaccgcg caggtgctgc
     2761 tggtccgggg cgaggccggc atcggcaaga cccgtctcct cgaggagctc aaccggatgg
     2821 cacgcgcgcg cggcttcgtg gggtatcgcg cggccgtgct cgatttcggg gtcggcagcg
     2881 gcggtggggc gatcgcatct ctggtgcgcg gcatgctcaa cgtgcatgag cgcgacagcc
     2941 ccgacaatct gcgcgccgcc gccgagcgcg ccatcgaggc tggcgaggtg gatcgcgagc
     3001 gcgctgcatt cctgtacggc atgctcgagc tcgacgtgcc ccaaccgctc gatcgcaccg
     3061 ccgtggatgc cggggcccgc cagcgcgcgg agg
//
LOCUS       DASRIE010000147         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3818044, whole genome shotgun sequence.
ACCESSION   DASRIE010000147 DASRIE010000000
VERSION     DASRIE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3818044"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFB53_06155"
     CDS             complement(<1..896)
                     /locus_tag="VFB53_06155"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZN86352.1"
                     /translation="MKRLILLVVVPAIALVGGLALWLQGGRFEETENAYVKAHMIAVT
                     ADVSGRVVEVGVRDNEPVAAGALLFRVDATPFELTVASAEAQMEVVRTELDGLRAEHR
                     VALADAAEAQERIGFLARQLERQAKLKERGMGLEASFDEARHNLEAARRRLDSSREKA
                     VRVLAALGGNVKVPTEAHPRFLAARAARDAGRDELARVRVVAPAAGIISNMRLQTGER
                     VERGVPVFSLIESRPMWVEANFKETQLTHMREGQEVTVVADAYPDARWRGRVSTIAPA
                     TKAEFALLPAQNSSGNWVKVVQR"
     gene            complement(893..1339)
                     /locus_tag="VFB53_06160"
     CDS             complement(893..1339)
                     /locus_tag="VFB53_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN86353.1"
                     /translation="MARVIREDLSRNFGYLVNDVARLMRTVYDRRVRTLGLTRSQWWV
                     LNHLFRHPGATQSELAAILEVERATLGRLLDRLQTKGWVRREDDAHDRRAWRVHLTAA
                     AEPLMRKLRKQAFGLRRDALAGLSARQREQFVDALLAIKSNLSRLK"
     gene            1371..>2292
                     /locus_tag="VFB53_06165"
     CDS             1371..>2292
                     /locus_tag="VFB53_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZN86354.1"
                     /translation="MTSSPPHRAWVLATCTAVTFLYSMSLTAVNVSLPQIQGALSATQ
                     DQIAWVITSNLVATAVMTPLTGWLAARFGARYLCLGCVAAFAAASAACGLAGSIETLV
                     AWRTVQGAAGAPLVPMSQAIVLASYPRERHGAVISIFGVASVMGPVVGPVLGGALAEA
                     YDWRAVFYMMVPFAVAALAACWLFIRDRQAPQPVRLDWTGFLMLSAALAGLQLMLDRG
                     ERAGWFSSAEIVAWAAVAALGLYLFIAHTLTASRPFLDPRLERNFALGLALIFVFGML
                     NFTPMTMLPPMLQGVAGYPDSVIGWVLGARG"
BASE COUNT          286 a          833 c          792 g          381 t
ORIGIN      
        1 cgctgcacca ccttgaccca gttgccgctg gagttttgcg ccggcagcag cgcgaactcg
       61 gcctttgtgg ccggcgcaat ggtgctgacg cggccgcgcc atcgggcatc cggataggcg
      121 tccgcaacca cggtgacttc ctgcccttcg cgcatatggg tgagctgggt ttccttgaaa
      181 ttggcctcga cccacattgg ccgcgactcg atcaggctga agaccggcac gccgcgctcg
      241 acgcgctcgc cggtctgcag gcgcatgtta ctgatgatgc cggcggccgg ggcgaccacg
      301 cgcacgcgcg cgagctcgtc gcgcccggcg tcgcgggcgg cgcgggcggc gaggaaacgc
      361 gggtgcgcct ccgttggaac cttgacgttg cccccgagcg cggcaagcac gcgcaccgcc
      421 ttctcgcgcg agctatcgag ccgccggcgc gcggcctcga ggttgtggcg cgcttcgtcg
      481 aagctcgcct cgagccccat gccgcgctcc ttcagcttcg cctggcgctc gagctggcgc
      541 gcgaggaagc cgatgcgctc ctgcgcttcc gcggcgtcgg cgagcgcgac gcggtgctcg
      601 gcgcgcaagc catcgagctc ggtgcgcacc acttccatct gcgcctccgc gctcgccacc
      661 gtgagctcga aaggcgtggc atcgacgcga aacagcaacg cgcccgcagc gaccggctcg
      721 ttgtcgcgca cgcccacctc caccacgcgc ccggatacgt cggcggtcac ggcgatcatg
      781 tgcgccttca cgtacgcgtt ctcggtttcc tcgaagcgcc cgccctgcag ccacagtgcc
      841 agtccgccga ccagggcgat ggccggcacc accaccagca ggataaggcg tttcatttca
      901 gccgcgacag gttcgacttg atcgcgagca gcgcatcgac gaattgctcg cgctgccggg
      961 cgctcaggcc ggccagggcg tcgcgccgca ggccgaaggc ctgcttgcgc agcttgcgca
     1021 tgagcggctc ggccgcggca gtgagatgca cccgccaggc gcgccggtca tgcgcgtcgt
     1081 cctcgcgccg cacccagccc ttggtctgca gccggtcgag caggcggccg agcgtggcgc
     1141 gctcgacctc gaggatggcg gcgagctcgg actgggtcgc gcccgggtgg cggaagagat
     1201 ggttcagcac ccaccactgc gagcgcgtca ggccgagggt gcgcacgcgc cggtcgtaga
     1261 ccgtgcgcat caggcgcgcg acgtcgttca ccaggtagcc gaagttgcgc gacaggtctt
     1321 cgcggatcac ccgggccatg ttagttgcct ggcttacaat gagcaaggca atgacctctt
     1381 ctcctccgca ccgggcatgg gtgctggcga cctgcaccgc ggtcaccttc ctctactcga
     1441 tgagcctcac cgcggtgaac gtgtcgctgc cgcagatcca gggcgcgctc tcggcgacgc
     1501 aggaccagat cgcgtgggtc attacctcga acctggtggc gaccgcggtg atgacgccgc
     1561 tcaccggctg gctcgccgcg cgcttcggcg cgcgttatct ctgcctcggg tgcgtcgccg
     1621 cctttgcagc ggcgtcggcc gcgtgcggcc tcgccggctc gatcgagacg ctggtggcgt
     1681 ggcgcacggt gcaaggcgcc gccggcgcgc cgctagtgcc gatgtcgcag gccatcgtgc
     1741 tggcaagcta tccgcgcgag cgccacggcg cggtgatctc catcttcggc gtggcctcgg
     1801 tcatgggccc ggtggtcggg ccggtgctcg gcggcgcgct cgccgaggcc tacgactggc
     1861 gcgcggtgtt ctacatgatg gtgccgttcg cggtggcggc gctcgccgcc tgctggctct
     1921 tcatccgcga ccgccaggcg ccgcagccgg tgcgcctgga ctggaccggc ttcctcatgc
     1981 tctcggccgc gctcgccggc ctgcagctga tgctggatcg cggcgagcgc gccggctggt
     2041 tctcgtccgc cgagatcgtc gcctgggcgg cggtggcggc gctcggcctg tacctcttca
     2101 tcgcgcacac gctcaccgcc tcgcggccgt tcctcgatcc gcgcctcgag cgcaacttcg
     2161 cgctcggcct cgcgctgatc ttcgtcttcg gcatgctcaa cttcacgccg atgacgatgc
     2221 tgccgccgat gctgcagggg gttgccggct atcccgactc ggtgatcggc tgggtgctcg
     2281 gcgcccgcgg cc
//
LOCUS       DASRIE010000148         7193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_155338, whole genome shotgun sequence.
ACCESSION   DASRIE010000148 DASRIE010000000
VERSION     DASRIE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155338"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..493
                     /locus_tag="VFB53_06170"
     CDS             146..493
                     /locus_tag="VFB53_06170"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZN86355.1"
                     /translation="MIAVIFEVEPRAGKKDAYLEIAARLRPQLEKMDGFLSVERFESL
                     TQPGKILSLSLWRDEEAVRGWRNLEEHRRAQESGRKSIFAGYRLRVAEVLRDYGMQER
                     AQAPQDSRAAHGG"
     gene            complement(495..1283)
                     /gene="pcaD"
                     /locus_tag="VFB53_06175"
     CDS             complement(495..1283)
                     /gene="pcaD"
                     /locus_tag="VFB53_06175"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZN86356.1"
                     /translation="MKAKTNGIETNYELHGKEGAPWLVLGHSLACSARMWDPQIEALK
                     GSYRILAYDTRGHGASGAPQGAYTLEMLADDLKALLDSLGVKHPHYCGLSMGGMIGQT
                     FALKYPGVFRTLTLADTSSRYPAEAWPLWQGRIKIAEEQGMVPLVQPTLERWFTEPFR
                     KSNPEAVEAIANLIASTPVAGYVGCCHAIPKIDVTARLKEIKCPVLVLCGDKDPGTPP
                     AMSEEIQRSTPGSKLVMIPQAAHLSNLEQPALFTKALREFLGAA"
     gene            complement(1280..2428)
                     /locus_tag="VFB53_06180"
     CDS             complement(1280..2428)
                     /locus_tag="VFB53_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN86357.1"
                     /translation="MRKPDYAGGGFANLMASVIEACGGAPRHAPLAALPARELAGARN
                     LMLLIVDGLGDNYLRREGAGGALLAGRRGAITSVFPSTTASAITTSYTGWTPLEHGLT
                     GWFTLFGAAGCVGAPLPFQRRGEKASLGTAPAAIYRAGPLFDGLARRSIVVSHSSIVD
                     STYNQHHCGGALRLAYDKLEGLVDQAVAAVNSGTDPKFVYVYWPEFDALSHRHGNGSA
                     EVRAQFRAVDAAYGELMARLSGTDTLVVATADHGFVDSTGADALALADAPGLAGLLRY
                     PLCGESRTVFCHVQSGREREFMQRAADWLGGRAEVRPSMELAEEGWYGGGDAHPRLAE
                     RIGDVTLLMHGRTSVLDWTPGERRHRHIGQHGGASEDEILIPLIVGSA"
     gene            2474..3037
                     /locus_tag="VFB53_06185"
     CDS             2474..3037
                     /locus_tag="VFB53_06185"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HZN86358.1"
                     /translation="MRLEVHVHADIPILEGVSRKQLEQAFAPWLEYLDADALADVKSL
                     EADEPGIRFDEKELVLLICWTGEIGRSFHAALESSLDNLGPYCYEAAEVDLTLYHENG
                     EQEAKLLFVGPTAQAIHEAQRRCMLEDIAALLERQFDKEAVAEVAALVNQLFDRDWKS
                     KSAAGGEKKQPERAFEYATRPGRKNLH"
     gene            3046..4338
                     /locus_tag="VFB53_06190"
     CDS             3046..4338
                     /locus_tag="VFB53_06190"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HZN86359.1"
                     /translation="MAGPKYPGFDTLALHAGQSPDPATGSRAVPVYLTTSYVFRDSEH
                     AASLFNMERAGHVYSRISNPTVAVLEERVAALESGVGAIATASGQAALHLAVATLMDA
                     GSHIVASNSLYGGSHNLLGYTMKRFGIETTFVAPGTPENFARAIRPNTRLLFGETLGN
                     PGLEVLDVPAVAQLAHEAGLPLLVDATFTTPVLMKPFELGADLLYHSATKFLGGHGTV
                     IGGVLVDSGRFDWEKSGKFPQLTAPYAGFHNMDFEEESGTRAFLLRARREGLRDFGAC
                     MAPMTAFQILQGIETLHLRMPRHVENTRKMVQFLAAQAAVEKVVYPELPGHPNHALAQ
                     KLLPRGCGAVFTFDLKGGREAGKRFIEALRVFSHLANVGDAKSLVIHPASTTHYRMTD
                     EDLKKAGIGPGTVRLSIGMEDAEDLLEDLGRALKASQK"
     gene            4335..5129
                     /locus_tag="VFB53_06195"
     CDS             4335..5129
                     /locus_tag="VFB53_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86360.1"
                     /translation="MNLAVDGRQAYAYTAAHALDAAKPAVVFVHGAGLDHSFWGLQSR
                     YFGYHGRNVLALDLPAHGRSAGPPLATIGEMADWVFRAMDAQQLPKAAIVGHSMGSLV
                     ALECAARAPGRVERIALLGTAYPMKVGEAFLEAARVNDQAAYDMETIWGHAPQVPLGG
                     NPNPGMWMYGDTLARLARLAPGVLHNDLRACNGYAGGEAAAAKVKCPVLFILGARDQM
                     TQAKAGLAFAQLLPGAKVVQLAPSGHSMMAESPDATLDALIAFLRA"
     gene            complement(5126..6463)
                     /locus_tag="VFB53_06200"
     CDS             complement(5126..6463)
                     /locus_tag="VFB53_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN86361.1"
                     /translation="MSNARARQLVEAPILPTIARLAAPGALLALFQTAVSVADTHYVG
                     RLGTDALAGLALVFPLVMLLQMTSAGAMGGGVSSALARALGAGNESAARRLVTHALVI
                     ALAAGLAFTALLLALGEVLYALLGGKGEALAQALAYSHVLFAGAVFVWLANTLASVLR
                     GGGNTLTPAVALAAAASVQIPLSGALTLGWGAFPRLGITGAALAYVAAFLVASLIMGA
                     VVWRGALRPRRADWAFEARHLREILRVGAVSSLGALQTVLTAVILTGFVGSFGTAALA
                     GYGVGVRLELLQIPLVFAIGQALVVMVGTNIGAGRGARAKRIAWTGTALAAGISLAIG
                     AFVAVFPGAWIRIFSDDPAVLATGSAYLRVVAPFYALFGAGMALYFASQGAGRMVLPV
                     LAGTARLVVVVTGGALVVLAAGPLTAVFAVIAGGIVLFGALIAAAVYRGAWGA"
     gene            complement(6460..6789)
                     /locus_tag="VFB53_06205"
     CDS             complement(6460..6789)
                     /locus_tag="VFB53_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN86362.1"
                     /translation="MRLLLVAGGWLYAAAIVFLSLTPRPPDPGFEYGDKLGHLAAYAL
                     LMFWFCWLYRRRVTRLAYGIGWVGLGIALEFAQAQTAYRSFEVGDMAANSLGVLLGWG
                     IFATLRR"
     gene            complement(6789..>7193)
                     /locus_tag="VFB53_06210"
     CDS             complement(6789..>7193)
                     /locus_tag="VFB53_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN86363.1"
                     /translation="GVLVAALIPALNDKTLEQVLEETARAGAQEAAYVILRLPNELKD
                     IFKEWLAAHYPERADHVISIVRQMRGGRDNDPRFGERMRGTGNFAELIASRFAVACRR
                     FGLNGQGGGRRRIELDCTRFVAPSPGGQMKLF"
BASE COUNT         1136 a         2558 c         2530 g          969 t
ORIGIN      
        1 gggcggtatc acgccacgat aggcttgcgc agcggcgttg atgatcgcca ggatcgccgg
       61 cgaatccgcc tcacggcttt ttcggatcat ggccaccacg gattgaacca tctaacattc
      121 gatggcgcca acccccggag agcccatgat cgccgtgatc ttcgaagtcg agccccgcgc
      181 aggaaagaag gacgcctacc tggaaatcgc cgcgcgcctg cggccgcagc tggagaagat
      241 ggacggcttc ctctcggtgg agcgcttcga gagcctcacg cagccgggca agatactttc
      301 attgtccttg tggcgcgacg aggaagcggt gcgcggctgg cgcaacctgg aggagcaccg
      361 ccgcgcgcag gaatccggcc gcaagtcgat cttcgccggc taccgccttc gcgtcgccga
      421 ggtgctgcgc gactacggca tgcaggagcg cgcgcaggcg ccgcaggact cgcgcgccgc
      481 gcacggcggc tgactcaagc cgcgccgagg aattcccgca gcgccttggt gaacagcgcc
      541 ggctgctcca ggttcgacag gtgcgccgcc tgcgggatca tcaccagctt cgagcccgga
      601 gtgctgcgct ggatctcctc ggacatcgcg ggcggggtgc ccgggtcctt gtcgccgcag
      661 agcaccagca ccgggcactt gatctccttc aggcgcgcgg tgacgtcgat cttcgggatg
      721 gcgtggcagc agccgacgta gcccgccacc ggcgtggaag cgatcaggtt ggcgatcgcc
      781 tcgaccgcct ccgggttgga cttgcggaac ggctcggtga accagcgctc cagcgtcggc
      841 tgcaccagcg gcaccattcc ctgctcctcg gcgatcttga tgcggccctg ccacagcggc
      901 caggcttccg ccgggtagcg gctgctggtg tcggcgagcg tgagcgtcct gaacacgccc
      961 ggatacttga gcgcgaaggt ctggccgatc atgccgccca tcgacaggcc gcagtagtgg
     1021 ggatgcttca cgccgaggga atcgagcagc gccttcaggt cgtcggcgag catctccagc
     1081 gtgtaggcgc cctgcggcgc cccggaagcg ccgtggccgc gcgtgtcgta ggcgaggatg
     1141 cggtagctcc ccttcagcgc ctcgatctgc gggtcccaca tgcgcgcgct gcaggcgagc
     1201 gagtggccga gcaccagcca gggcgcgcct tccttgccgt gcagttcgta gttggtctcg
     1261 atgccgttgg tcttcgcttt catgcgctcc ccacgatgag tggaatcagg atctcgtctt
     1321 cgctcgcgcc gccgtgctgg ccgatgtggc ggtggcgccg ctcgccgggc gtccagtcga
     1381 gcacgctcgt gcggccgtgc atcagcagcg tcacgtcgcc gatgcgctcg gcgaggcgcg
     1441 gatgcgcgtc gccgccgccg taccagccct cctcggcgag ctccatgctc ggtcgcacct
     1501 cggcgcgccc gcccagccag tcggccgcgc gctgcatgaa ttcgcgctcg cgcccggatt
     1561 gcacgtggca gaacacggtg cggctctcgc cgcacagagg atagcgcagc agcccggcga
     1621 ggccgggcgc gtcggcgagc gcgagcgcgt ccgcccccgt gctgtcgacg aagccgtggt
     1681 cggcggtggc caccaccagc gtgtcggtgc cggagagccg cgccatcagc tcgccatagg
     1741 cggcgtccac cgcccggaac tgcgcgcgca cctcggcgct gccgttgccg tggcggtgcg
     1801 acagcgcgtc gaactccggc cagtagacgt agacgaactt gggatccgtg cccgaattca
     1861 ccgcggccac cgcctgatcc accaggcctt cgagcttgtc gtaggccagg cgcaaggcgc
     1921 cgccgcagtg gtgctggttg taggtcgaat cgacgatgga gctgtgcgag accacgatcg
     1981 agcggcgcgc gagtccgtcg aacagcgggc cggcgcggta gatggcggcg ggcgccgttc
     2041 cgagggaagc cttctcgccg cggcgctgga aaggcagcgg cgcgccgacg cagcctgccg
     2101 cgccgaacag ggtgaaccag ccggtgagcc cgtgctcgag cggcgtccag ccggtgtacg
     2161 aggtggtgat cgcgctcgcc gtggtggagg gaaacaccga ggtgatcgcg ccgcgccggc
     2221 ccgcgagcag cgcgccgccg gcgccctcgc gccgcaggta gttgtcgccc aggccgtcga
     2281 cgatcagcag catgagattg cgcgcgccgg cgagctcgcg cgcgggcagc gcggcgagcg
     2341 gggcgtggcg cggcgcgccg ccgcaagcct cgatgaccga ggccatcagg ttggcgaaac
     2401 cgcccccggc gtagtccggt ttgcgcatgc ccgcatgata aacttcgcct ccgtcaacgt
     2461 cgcgaggttt cccatgcggc tcgaagtcca cgtgcacgcc gacatcccca tcctcgaagg
     2521 ggtttcccgc aagcagctcg aacaggcttt cgcgccctgg ctcgagtacc tcgacgccga
     2581 cgcgctcgcc gacgtgaaga gcctggaagc cgacgagccg ggcatccgct tcgacgagaa
     2641 ggaactggtg ctgctgatct gctggaccgg cgagatcggc cgcagcttcc acgccgcgct
     2701 cgaatcctcg ctcgacaacc tcggccccta ttgctacgag gcggccgagg ttgacctcac
     2761 cctgtaccac gagaacggcg agcaggaagc caagctcctg ttcgtcgggc cgacggcgca
     2821 ggcgatccac gaggcgcagc ggcgctgcat gctcgaggac atcgccgcgc tgctcgagcg
     2881 ccagttcgac aaggaagccg tggccgaggt ggcggcgctg gtcaaccagc tgttcgaccg
     2941 cgactggaag agcaagtccg cggcgggcgg cgagaagaag cagcccgagc gcgccttcga
     3001 gtacgccacg cgcccggggc gcaagaacct gcactgattt tttaggtggc cggcccgaaa
     3061 tatccggggt tcgacactct cgcgctgcac gcgggccagt cgcccgaccc ggcgaccggc
     3121 agccgcgcgg tgccggtgta cctcaccacc tcctacgtgt tccgcgacag cgagcacgcc
     3181 gcgtcgctgt tcaacatgga gcgcgcgggc cacgtctact cgcgcatctc caatcccacg
     3241 gtagcggtgc tggaggagcg cgtcgcggcg ctcgagagcg gcgtgggcgc gatcgccacc
     3301 gcctccggcc aggcggcgct gcacctcgcg gtggccacgc tgatggacgc gggcagccac
     3361 atcgtggcct cgaattccct gtacggcggc tcgcacaacc tgctcggcta caccatgaag
     3421 cgcttcggca tcgagacgac gttcgtcgcg ccgggcacgc cggagaattt cgccagggcc
     3481 atccgcccca atacgcgtct cctgttcggc gagacgctgg gcaaccccgg gctggaagtg
     3541 ctcgacgtcc cggcggtggc gcagctggcg cacgaggcgg gactgccgct gctggtggac
     3601 gccaccttca ccacgccggt cctgatgaag cctttcgagc tgggcgcgga cctgctctac
     3661 cactcggcga ccaagttcct cggcggccac ggcacggtga tcggcggcgt gctggtggac
     3721 tcgggccgct tcgactggga gaagagcggc aaattcccgc agctcaccgc gccctacgcc
     3781 ggcttccaca acatggattt cgaggaggag tcgggcacgc gcgcgttcct cctgcgcgcg
     3841 cggcgcgagg gcctgcgcga tttcggcgcc tgcatggcgc cgatgaccgc cttccagatc
     3901 ctgcagggca tcgagaccct ccacctgagg atgccgcgcc acgtggagaa cacgaggaag
     3961 atggtgcagt tcctcgccgc gcaggcggcg gtggagaagg tggtctatcc cgagctgccg
     4021 ggccacccga accatgcgct ggcgcaaaag ctcctgccca ggggctgcgg cgcggtgttc
     4081 accttcgacc tgaaaggcgg gcgcgaagcg ggcaagcgct tcatcgaggc gctgcgcgtg
     4141 ttctcgcacc tcgccaacgt cggcgacgcc aagtcgctgg tcatccatcc ggcgagcacc
     4201 acgcactacc gcatgacgga cgaggacctg aagaaggccg gcatcgggcc cggcaccgtg
     4261 cgcctgtcga tcggcatgga agacgcggaa gacctgctcg aggacctcgg gcgcgcgctg
     4321 aaggcctcgc agaagtgaac cttgccgtcg acggccggca ggcctacgcc tacaccgcgg
     4381 cgcatgcgct cgacgcggcg aagcccgccg tcgtcttcgt gcacggcgcg gggctggacc
     4441 attccttctg gggactgcag agccgctact tcggctacca cggccgcaac gtcctcgcgc
     4501 tcgacctgcc ggcgcacggc cgctcggcgg gaccgccgct cgccaccatc ggcgagatgg
     4561 cggactgggt gttccgcgcg atggacgcgc agcagctgcc gaaggcggcc atcgtcggcc
     4621 attcgatggg gtccctggtg gcgctggagt gcgccgcgcg cgcgcccggg cgcgtcgagc
     4681 gcatcgcgct gctcggcacc gcctacccga tgaaggtggg cgaggccttc ctcgaggcgg
     4741 cgagggtgaa tgaccaggcc gcctacgaca tggagaccat ctggggccat gcgccgcagg
     4801 tgccgctcgg cggcaacccc aaccccggca tgtggatgta cggcgacacg ctggcgcgcc
     4861 tcgcgcgcct ggcgcccggc gtgctgcaca acgacctcag ggcctgcaac ggctacgccg
     4921 gcggcgaagc cgcggcggcg aaagtgaaat gcccggtgct gttcatcctg ggcgcgcgcg
     4981 accagatgac gcaggccaag gccggcctcg cgttcgcgca gctgctgccc ggcgcgaaag
     5041 tggtgcagct cgcgccctcc ggccattcga tgatggccga atcgcccgac gcgacgctgg
     5101 acgcgctgat cgccttcctg cgcgcctagg cgccccaggc tccgcggtac acggccgcgg
     5161 cgatcagcgc gccgaacagc acgatgccgc cggcgatgac cgcgaacacc gccgtgagcg
     5221 gcccggcggc gagcaccacc agcgcgccgc cggtcacgac gacgaccagg cgcgccgtgc
     5281 cggccagcac cggcagcacc atgcgcccgg cgccctgcga agcgaagtac agcgccatgc
     5341 cggcgccgaa cagcgcgtag aacggcgcca cgacgcgcag gtacgcgctg ccggtggcga
     5401 gcaccgccgg atcgtcgctg aagatgcgga tccaggcgcc gggaaacacc gcgacgaacg
     5461 ccccgatggc gagcgagatg ccggcggcaa gcgcggtccc cgtccaggcg atgcgcttcg
     5521 cgcgcgcgcc gcggccggcg ccgatgttgg tgccgaccat gaccaccagc gcctggccga
     5581 tggcgaacac cagcgggatc tgcagcagct cgaggcgcac gcccacgccg tagccggcga
     5641 gcgccgcggt gccgaagctg ccgacgaagc cggtgaggat caccgccgtg agcaccgtct
     5701 gcagcgcgcc gagcgaggac accgccccca cgcgcaggat ctcgcgcagg tgccgcgctt
     5761 cgaacgccca gtcggcgcgc cgcgggcgca gcgcgccgcg ccacaccacc gcgcccatga
     5821 tcaggctggc gacgaggaag gcggcgacat aggcgagggc cgcccccgtg atgcccagtc
     5881 gcgggaacgc tccccagccg agcgtcagcg cgcccgatag cgggatctgc accgaggccg
     5941 cggcagccag ggcgaccgcc ggggtgagcg tgttgccgcc gccgcgcagc acgctcgcca
     6001 gggtattcgc cagccagacg aacaccgcgc cggcgaacag cacgtgcgaa taggcgagcg
     6061 cctgcgccag cgcctcgccc ttgcccccga gcagcgcgta gagcacttcg cccagcgcga
     6121 ggagcagcgc ggtgaacgca agtcccgcgg cgagcgcgat caccagcgcg tgggtcacca
     6181 gccgccgcgc cgcggattcg tttccggcgc cgagcgcacg cgccagcgcg gaggacaccc
     6241 cgccgcccat ggcgccggcg gaggtcatct gcagcagcat caccagcgga aagacgagcg
     6301 cgagaccggc aagggcgtcc gtgcccaggc gcccgacgta gtgcgtgtcg gcgactgaca
     6361 ccgcggtctg gaacagcgcg agcagcgcgc cgggcgccgc gagccgcgcg atggtcggca
     6421 ggatgggcgc ctccaccagc tgccgggcgc gtgcattgct catcgccgca gcgtagcgaa
     6481 gattccccag ccgagcagca cgcccagcga attggcggcc atgtcgccga cttcgaagct
     6541 gcggtaagcg gtctgggcct gcgcgaactc cagggcgatg cccaggccga cccagccaat
     6601 gccgtaggca agccgcgtca cccgacgacg atagagccag cagaaccaga acatcagcag
     6661 cgcgtaggcg gcgaggtggc cgagcttgtc gccgtactcg aagcccggat cgggcgggcg
     6721 gggggtgagc gagaggaaca cgatcgcggc ggcgtacagc cagccgccgg cgacgagcag
     6781 caggcgcatc agaacagctt catctgcccg ccgggcgagg gcgcgacgaa gcgcgtgcag
     6841 tccagctcga tcctgcggcg cccgccgcct tggccgttga ggccgaagcg cctgcaggcg
     6901 acggcaaagc ggctggcgat cagctcggcg aagttccccg tgccgcgcat gcgctcgccg
     6961 aagcgcgggt cgttgtccct gccgccgcgc atctggcgca cgatgctgat cacgtggtcg
     7021 gcgcgttccg gataatgcgc ggcgagccac tccttgaaga tgtccttcag ttcattgggc
     7081 agacgcagga tcacgtaggc cgcttcctgc gcgccggccc tggccgtttc ctccagcacc
     7141 tgctcgaggg tcttgtcgtt gagcgccggg atcagcgccg ccaccagcac gcc
//
LOCUS       DASRIE010000149         5988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_933548, whole genome shotgun sequence.
ACCESSION   DASRIE010000149 DASRIE010000000
VERSION     DASRIE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933548"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /gene="glyS"
                     /locus_tag="VFB53_06215"
     CDS             complement(<1..1611)
                     /gene="glyS"
                     /locus_tag="VFB53_06215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZN86364.1"
                     /translation="MAKKAMAPPRAETLLVELLTEELPPRALEALALAFRDNLTNLLE
                     AAGLRSSQSAARCFATPRRLAVAITHVLAVAPAVREKVQGPSVKAPPEAVAGFARKHG
                     VEPDTMLRLETSKGFVYAVERTVPGAQLAEVLAPMVESALAALPAPKTMRWGAGEARF
                     VRPVHGLVMLHGAAVVAGTALGVAAGRRTRGHRFMAKGWITLRDADSYEAPLREEGRV
                     LADFGARRAEIERQLQAAAGALRATLGEYQDLLGEVTALVEHPSVYLGAFDKAFLQIP
                     QECLVLTMRQNQKYFPLFDAAGALLPKFLIVSNMQVDDPRHIIGGNERVVRPRLEDAR
                     FFYDQDRRTRLEERLPLLAKVVFHHKLGHQLDRVQRMKILAGMVARSLGADDKLAERA
                     AELAKADLLTGMVGEFPELQGVMGRYYALHDGEPPEVADAIEAHYRPRFAGDKLPEHP
                     VACAVALADKLYVLAGFFGAGQQPTGDKDPYGLRRAALGVIRILVERAMPVSLHDLVN
                     AAYQVLPKDIAQAHKEVEVFARERMRGYL"
     gene            complement(1616..2500)
                     /gene="glyQ"
                     /locus_tag="VFB53_06220"
     CDS             complement(1616..2500)
                     /gene="glyQ"
                     /locus_tag="VFB53_06220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZN86365.1"
                     /translation="MTAPTFQQLISRLNEFWGRQGCALLQPYDMEVGAGTFHTATFLR
                     AVGPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVMKPSPPDIQERYLASLAAL
                     GIEAQAHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLECKPVLGEI
                     TYGLERLALYLQDKASIYDLVWTPGVSYGDVYHQNEVEQSRYNFELSSPELLFRHFGD
                     YETEAKRLIEARCTLPAYEMVLKCSHTFNLLDARGAISVTERAAYIGRVRSLARAVAQ
                     AYYEARERLGFPMTLGRT"
     gene            complement(2513..3973)
                     /gene="lnt"
                     /locus_tag="VFB53_06225"
     CDS             complement(2513..3973)
                     /gene="lnt"
                     /locus_tag="VFB53_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN86366.1"
                     /translation="MRRRPLVAFVAGAAMVPAHAPLDLFPLALLSLAVLTQLWVTARG
                     AREAAWLGFCFGLGTFLAGVSWVYISLHRFGAMPAPLAAVATLAFCAVLALYPALAGA
                     VQSRLRAAPALRAAVVIPACWTLAEWLRATLFTGFPWLALGTATVDTPLAGFAPLGGV
                     YATTLAAAVCAGLLWCVALGQARWTAALAFAVLGAGGAALRDVPWTEPAGAPLAASVL
                     QGNVPQDLKFDPQRYARTAETYARLAEGSRAQLIVLPETALPRMLDRIEPATLDRLEA
                     AARRNGGDLLLGVPVRAAPEETYNAVLSLGTAPRQLYYKSHLVPFGEFVPPGFGWVVR
                     VLSIPLADFTRGPARPAPLRVAGQSVAVNICYEDAYGAELARQLPEATLLVNVSNVAW
                     FGASLAPAQHLQIARARALETGRAHLAATNTGITAAIGRDGRVLARLAPFTEGRLDVS
                     VQGYRGATPYVRWGDWPALAACLAALAVVALRVRSR"
     gene            complement(3973..4794)
                     /locus_tag="VFB53_06230"
     CDS             complement(3973..4794)
                     /locus_tag="VFB53_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZN86367.1"
                     /translation="MNDPTRPSLLERLSALLLREPEDREQLLSLLRSAYQRNLLDADA
                     LSMIEGAMTVSEMPVRDIMVPRAQMDVIDIHHRVEDFITQIIATAHSRFPVIDQNRDD
                     VIGILLAKDLLRHYAGEEEFNVREMLRPAIFVPESKRLNVLLREFRASRNHMAIVVDE
                     YGGVAGLLTIEDVLEQIVGDIEDEYDFDEASDNILLESSGRYRVKALTQIADFNAAFG
                     TAYSDQDHDTVGGLLIARLGRLPKRGEASIIDGFKFQVLRADSRRVYTLLVDKPK"
     gene            complement(4805..5167)
                     /gene="ybeY"
                     /locus_tag="VFB53_06235"
     CDS             complement(4805..5167)
                     /gene="ybeY"
                     /locus_tag="VFB53_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZN86368.1"
                     /translation="MRAWALAAAPAGAEVTLRVVGGAEARRLNRDFRGRDYATNVLSF
                     SYAGAQGDIVLCHPVIAREARAQGKALAAHYAHLVVHGMLHLRGRDHRRAADARRMER
                     AEVRILRRLGFGDPYALK"
     gene            complement(5223..5888)
                     /locus_tag="VFB53_06240"
                     /pseudo
     CDS             complement(5223..5888)
                     /locus_tag="VFB53_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
BASE COUNT          843 a         2110 c         2209 g          826 t
ORIGIN      
        1 gaggtagccg cgcatgcgct cgcgcgcgaa cacctccacc tccttgtgcg cctgggcgat
       61 gtccttgggc agcacctggt aggcggcgtt caccaggtcg tgcagcgaca ccggcatggc
      121 gcgttccacc aggatgcgga tcacccccag cgccgcgcgg cgcaggccgt acgggtcctt
      181 gtcgccggtg ggctgctggc cggcgccgaa aaatcccgcc aggacgtaca gcttgtcggc
      241 gagcgccacg gcgcaggcca ccgggtgctc gggcagcttg tcgccggcaa agcgcggacg
      301 gtagtgcgcc tcgatggcgt cggcgacctc gggcggctcg ccgtcgtgca gcgcgtagta
      361 gcgtcccatc acgccttgca gctccgggaa ctcgcccacc atgccggtga ggaggtcggc
      421 cttggcgagt tcggcggcgc gctcggcgag cttgtcgtcg gcgccgaggg agcgcgccac
      481 catgccggcg aggatcttca tgcgctgcac gcggtcgagc tggtggccga gcttgtggtg
      541 gaacaccacc ttggcgagca gcggcaggcg ctcctccagg cgcgtcctgc ggtcctggtc
      601 gtagaagaag cgcgcgtcct ccagccgcgg gcgcaccacg cgctcgttgc cgccgatgat
      661 gtggcgcggg tcgtcgacct gcatgttgga gacgatgagg aacttcggca gcagggcgcc
      721 cgcggcgtcg aacagcggga agtacttctg gttctggcgc atggtgagca ccaggcattc
      781 ctgcgggatc tgcaggaagg ccttgtcgaa ggcgcccagg tagacgctcg ggtgctcgac
      841 cagcgcggtc acctcgccca gcaggtcctg gtactcgccg agcgtggcgc gcagcgcgcc
      901 ggccgcggcc tgcagctggc gctcgatctc ggcgcggcgc gccccgaaat cggccagcac
      961 cctgccttcc tcgcgcagcg gcgcctcgta ggaatccgcg tcgcgcagcg tgatccagcc
     1021 tttggccatg aagcggtggc cgcgcgtgcg gcggccggcg gcgacgccca gcgccgtacc
     1081 ggccaccacc gccgcgccgt gcagcatcac caggccgtgc accggccgca cgaagcgcgc
     1141 ctcgcccgcg ccccagcgca tggtcttcgg cgccggcagc gcggcgagcg cggactcgac
     1201 catgggcgcc agcacctcgg caagctgcgc gcccgggacg gtgcgctcca cggcgtagac
     1261 gaagcccttg ctggtctcca gccgcagcat ggtgtcgggc tcgaccccgt gcttgcgcgc
     1321 gaacccggcc accgcctcgg gcggggcctt gaccgagggt ccctgcactt tctcgcgcac
     1381 cgccggcgcc actgccagca cgtgcgtgat ggcgacggcg agccgccgcg gcgtggcgaa
     1441 gcagcgcgcc gcgctctggc tgctgcgcag cccggcggcc tccagcaggt tggtgaggtt
     1501 gtcgcggaag gcgagcgcga gcgcctccag cgcgcgcggc ggcagctcct cggtgagcag
     1561 ctcgacgagc agcgtctcgg cccgcggcgg cgccatggct ttcttcgcca tcgccctagg
     1621 tccggcccag ggtcatgggg aagccgagcc gctcgcgcgc ctcgtagtag gcctgcgcca
     1681 ccgcgcgcgc gagcgagcgc acgcggccga tgtaggcggc gcgctcggtg acggagatgg
     1741 cgccgcgggc gtccagcagg ttgaaggtgt gcgagcactt gagcaccatc tcgtaggcgg
     1801 gcagggtgca gcgcgcctcg atgaggcgtt tcgcctcggt ctcgtaatcg ccgaaatggc
     1861 ggaacagcaa ttcggggctg gacagttcga agttgtagcg cgactgctcc acctcgttct
     1921 ggtggtacac gtcgccgtag ctcacgcccg gcgtccacac caggtcgtag atcgacgcct
     1981 tgtcctgcag gtacagggcg aggcgctcca ggccgtaggt gatctcgccc agcaccggct
     2041 tgcattccag cgagcccact tcctggaagt aggtgaattg cgtcacctcc atgccgtcga
     2101 gccacacctc ccagcccagg ccccaggcgc ccagggtggg cgactcccag tcgtcctcga
     2161 cgaagcggat gtcgtgcgcc tgcgcctcga tgccgagcgc ggcgagcgag gcgaggtagc
     2221 gctcctggat gtcgggcggc gagggcttca tcaccacctg gtactggtag tagtgctgca
     2281 ggcggttggg attctcgccg tagcgcccgt ccttggggcg gcgcgagggc tgcacgtagg
     2341 cggcgttcca gggctccggg ccgacggcgc gcaggaaggt cgcggtgtgg aaggtgccgg
     2401 cgccgacttc catgtcgtag ggctgcagca gcgcgcagcc ctggcggccc cagaactcgt
     2461 tcaggcgcga aatgagttgc tggaaggtcg gcgcggtcac ggtgccggaa tcttaccggc
     2521 tgcgcacgcg cagcgcgacg acggcgagcg cggcgaggca ggcggcgagc gccggccagt
     2581 cgccccagcg cacgtagggc gtcgcgccgc ggtagccctg cacgctcacg tccagccgcc
     2641 cctcggtgaa cggcgcaagg cgcgccagga cgcgcccgtc gcggccgatc gccgcggtga
     2701 tgccggtatt ggtggcggcg aggtgcgcgc ggccggtctc cagtgcgcgc gcgcgcgcga
     2761 tctgcaggtg ctgcgccggg gcgagcgaag cgccgaacca ggcgacgttg gacacgttca
     2821 ccagcagcgt cgcctcgggc agctggcggg ccagctccgc gccgtaggcg tcctcgtagc
     2881 agatgttcac cgcgacgctc tggcccgcga cgcgcagcgg cgcggggcgc gccggcccgc
     2941 gcgtgaaatc ggccagcgga atggacagca cgcgcaccac ccagccgaag cccggcggca
     3001 cgaactcgcc gaacggcacc aggtgcgact tgtagtagag ctggcgcggc gccgtgccga
     3061 ggctgagcac ggcgttgtag gtctcctcgg gcgcggcgcg caccggcacg ccgagcagca
     3121 ggtcgccgcc gttgcgacgg gccgccgctt cgagccggtc gagggtggcc ggctcgatgc
     3181 ggtcgagcat gcgcggcagc gcggtttccg gcagcacgat gagctgcgcg cggctgcctt
     3241 cggcgaggcg cgcgtaggtc tccgcggtgc gcgcatagcg ctgcgggtcg aacttcaggt
     3301 cctgcggcac gttgccctgc agcacgcttg cggcgagcgg cgctccggcg ggctccgtcc
     3361 agggcacgtc gcgcagcgcc gcgccgccgg cgccgagcac ggcgaaggca agggccgccg
     3421 tccagcgcgc ctggccgagg gccacgcacc acagcaggcc ggcgcacacg gcggcggcga
     3481 gcgtcgtggc gtacacgccc cccagcggcg cgaagccggc gagcggcgtg tccaccgtgg
     3541 cggtgcccag cgccaaccag ggaaagcccg tgaacagggt cgcccgcagc cactcggcga
     3601 gcgtccagca ggccgggatc accaccgcgg cacgcagcgc gggcgccgcg cgcagcctcg
     3661 actggaccgc gccggcaagc gccgggtaga gcgcgagcac ggcgcaaaag gcgagcgtgg
     3721 caactgcggc cagaggcgcc ggcatggcgc cgaagcggtg caggctgatg tacacccagg
     3781 acacgccggc gaggaaggtt cccaggccga agcagaaacc cagccaggcc gcctcgcgcg
     3841 cgccgcgcgc cgtcacccac agctgggtga gcaccgccag cgagagcagc gcgagcggga
     3901 acagatcgag cggcgcatgc gccggcacca tcgccgcgcc ggccacgaac gcgacgaggg
     3961 gccggcgacg catcacttgg gcttgtcgac cagcagcgtg tagacgcgcc ggctgtcggc
     4021 gcgcagcacc tggaacttga agccgtcgat gatgctcgcc tcgccgcgct tgggcaggcg
     4081 cccgaggcgc gcgatcagca ggccgcccac cgtgtcgtgg tcctggtcgg agtaggcggt
     4141 gccgaaggcg gcgttgaagt cggcgatctg ggtcagcgcc ttgacgcggt agcggccgct
     4201 cgactcgagc aggatgttgt cgctcgcctc gtcgaagtcg tactcgtcct cgatgtcgcc
     4261 gacgatctgc tccagcacgt cctcgatggt cagcaggccc gccacgccgc cgtactcgtc
     4321 caccacgatc gccatgtggt tgcggctggc gcggaactcg cgcagcagca cattgaggcg
     4381 cttggactcg ggcacgaaga tcgccgggcg cagcatctcg cgcacgttga actcctcctc
     4441 gccggcgtaa tggcgcagca ggtccttggc gagcaggatg ccgatgacgt cgtcgcggtt
     4501 ctggtcgatc accgggaagc gcgagtgcgc ggtggcgatg atctgcgtga tgaagtcctc
     4561 gacccggtgg tggatgtcga tcacgtccat ctgcgcgcgc ggcaccatga tgtcgcgcac
     4621 cggcatctcg gacaccgtca tcgcgccctc gatcatgctg agggcgtcgg cgtccagcag
     4681 gttcctctgg tacgccgagc gcagcagcga cagcagctgc tcgcggtcct cgggttcgcg
     4741 caggaggagc gcggagagcc gctccagcag gctgggtcgt gtggggtcgt tcatgccttg
     4801 cggctcattt tagcgcgtag gggtcgccga acccgagccg gcgcaggata cggacctcgg
     4861 cgcgttccat gcgccgggcg tcggcggcgc gccggtggtc gcggccgcgc agatgcagca
     4921 tgccgtgcac caccaggtgc gcgtagtgcg cggcgagcgc cttgccctgc gcgcgcgcct
     4981 cgcgcgcgat caccgggtgg cacagcacga tgtcgccctg cgccccggca taggaaaacg
     5041 acagcacgtt ggtggcgtag tcgcgcccgc ggaagtcgcg gttcaggcgg cgcgcctcgg
     5101 cgccgccgac cacgcgcagc gtgacctccg cgccggcggg cgcggccgcc agcgcccagg
     5161 cgcgcaggcg cgccgcggag ggcgcgccgc cccccgctac cccggtgcgc tgcaccgcga
     5221 cgctactgcg gcggcgcggc gcttttctcg tacgcatcga tgatgcgcgc gaccagcggg
     5281 tggcgcacga cgtcggcggc ggtgaaatcg gtgaaggcga tgccgcgcac cgcggcgagg
     5341 atgcgccgcg cctcgatcag gccgctcttc tgcccgcggc ccaggtcgat ctgcgtcacg
     5401 tcgccgttga ccaccgcctt ggcgccgaag ccgatgcgcg tgaggaacat cttcatctgc
     5461 tcgggcgtgg tgttctgcgc ctcgtccagg atcacgaagg cgtggttcag cgtgcggccg
     5521 cgcatgtagg cgagcggcgc cagctcgatg gcgccgcgct ccaggagctt gcccaccttg
     5581 tcgaagccca tcaggtcgta cagcgcgtcg tacagcggcc gcaggtaggg atcgaccttg
     5641 gcctggagat ccccgggcag gaagccgagc gactcgcccg cctccaccgc cggacgcgcg
     5701 aggatgatgc gcttgacccg cttccgggcc agcgcctcga ccgcgggtcg caccagcacg
     5761 atccgcttca ccgcgtcgcg ttcgagcgca tcgacggcgc aggcgacggc gaggtaggtc
     5821 ttgccggttc ccgcggggcc gacgccgaac gtgatgtcgt gcgcctggcg gccgacctcc
     5881 tttcgcggga cagcgagacg cggctgcgcg actacctgct caagtcggca gtgcgcggcg
     5941 gcaagcgcgt ggtcgttccg cgcagcctca accagcgctc gtacctcg
//
LOCUS       DASRIE010000150        33143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1208184, whole genome shotgun sequence.
ACCESSION   DASRIE010000150 DASRIE010000000
VERSION     DASRIE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 33143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208184"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFB53_06245"
     CDS             <1..287
                     /locus_tag="VFB53_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN86369.1"
                     /translation="MLRHVLPNVTGPVLVIATIHVATAIITEATLSFLGVGVPPTQPS
                     LGTLIRIGNDFLISGEWWITVFPGVALVVLVLSVNLLGDWLRDALNPKLR"
     gene            284..1267
                     /locus_tag="VFB53_06250"
     CDS             284..1267
                     /locus_tag="VFB53_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86370.1"
                     /translation="MSPAPLLEVRGLRVEFPTRRGTLVAVDDLSFAIAPGEVLGVVGE
                     SGAGKSLTGMAIIGLLEPPGRIAAGEIRLEGERIDQLPPERMRRIRGRRIGAIFQDPL
                     TSLNPLYTVGDQLVETILTHLEVDRDEARDRALKLLAEVGITAPAARIDHYPHQFSGG
                     MRQRVVIALALCAEPSLIVADEPTTALDVSIQAQVIALLKRLAREHGTAVMLITHDMG
                     VIAETADRVAVMYAGRLAELGPVREVIHQPRHPYTAGLMGSIPKIGALKRHDRLVQID
                     GAMPRLNAIPAGCAFNPRCPKRFERCFAERPEPLQVGNALASCWLYADARR"
     gene            1254..2252
                     /locus_tag="VFB53_06255"
     CDS             1254..2252
                     /locus_tag="VFB53_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN86371.1"
                     /translation="MHAASRPLVHLTDVGRDFDVSKPWLNRLVEGAPRQLLRAVDGVS
                     FDVKRGETLALVGESGCGKSTVARLIVGLYALSRGRIEFDGIDLAAPGTDAPALRRRM
                     QMIFQDPYASLNPRWRVRDIVAEPIRVLGLAASPQAERDRVAELLRQVGLAPEDGEKY
                     PHEFSGGQRQRISIARALSGNPEFLVCDEPTSALDVSVQAQILNLMTELQERLGLTYL
                     FISHNLAVVSHIADRVGVMYLGRVVELAGAAELFAAPRHPYTRMLMDAIPDLQMTGKA
                     RTPVAGEVPNPLAPPAGCAFHPRCPHANARCRSERPLLTGSGAAAVACHAVEERRL"
     gene            complement(2249..4048)
                     /locus_tag="VFB53_06260"
     CDS             complement(2249..4048)
                     /locus_tag="VFB53_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZN86372.1"
                     /translation="MKWLKVSLITLSGLLLLLVAGAAILALSFDPNQYKDEVEKLVKE
                     KSGRTLKFHGDVKLAFWPSIGVAVGKVTLSRRASAHDFAAFDAAHVSVRLLPLLRGEV
                     LVDQVRVAGLKASVIRAKGGKFDFEDLLGAVGAKPPAKAPPQAGKVKFDIAGIRLEDG
                     TLAYSDEGSGQTLEIAHLDLSTGRIAEDVPGRMSLGAALKGKNPLVDAKVALEAGYRL
                     SFARQEYALSGLALKVEGTAADFSRLALALSGDVRADVAKESVNADLTVKFDDTTIKA
                     KTGMAGFDAPRYSFDLDVDRINLDRYLVASPKAKPAPGANAKVDVDVPVDLGALKGLR
                     ATGRLAVGELQLQGLKLAELKAQLRAADGRAELAPHSAKLYEGTLQGALTLEAGGNRV
                     AMKESLANVAVGPLIKDLMGRDAVEGRGDVTLDLAAAGATVNAMKKSLGGTARVALRD
                     GALKGFNLTEALRRTKAAFGSKSAQQQSADPSQRTDFSEMTASFAIKGGVAHNEDLDV
                     KAPLVRLGGAGDIDIGNSQLNYVARATVVATSKGQGGADLDHLAGLTVPVKLSGPFDA
                     PKVEIDYRSLATGAAKAKAKEKVKDKLRDLLKR"
     gene            complement(4045..4947)
                     /locus_tag="VFB53_06265"
     CDS             complement(4045..4947)
                     /locus_tag="VFB53_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HZN86373.1"
                     /translation="MPVNFDGKLVVAISSRALFDFEEENRIFDRDGEAAYVALQMGRI
                     DVPAKQGVAFPLVKKLLAFNAPDAQRVEVVILSKNDPVSGLRVFRSAQRAGLRVERGV
                     FTRGRQPYKYLDALRANLFLSANEQDVMRALDAKIPAARVVPESAQAASRHLDEVRIA
                     FDGDAVLFSDEAERVYQADGLEAFTRHETRHVDTPLPPGPFKPLLEALHRLQTAGTPM
                     RLRTALVTARSAPAHERAVRTLMHWKIAVDEALFLGGLDKGEFLKAFEPDFYFDDQRG
                     HVESAGKHVATGHVPFGVAGRKPA"
     gene            complement(4953..5026)
                     /locus_tag="VFB53_06270"
     tRNA            complement(4953..5026)
                     /locus_tag="VFB53_06270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4992..4994),aa:Gly,seq:ccc)
     gene            5072..5260
                     /gene="thiS"
                     /locus_tag="VFB53_06275"
     CDS             5072..5260
                     /gene="thiS"
                     /locus_tag="VFB53_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZN86374.1"
                     /translation="MNGSAHRLERPLDVAALIARLELSGKRIAVERNGEIVPKSAHGS
                     TLVADGDRLEVVVAVGGG"
     gene            5257..6060
                     /locus_tag="VFB53_06280"
     CDS             5257..6060
                     /locus_tag="VFB53_06280"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZN86375.1"
                     /translation="MSEAFVIAGKAYTSRLLIGTGKYRDFAQTRAAVEASGAEIVTVA
                     IRRTNIGQSAGEPSLLEALPPSKYTYLPNTAGCYSAEDAVRTLRLARELLDGHDLVKL
                     EVLGDPHTLYPNMPETLAAAKQLVREGFKVMVYCSDDPIQAKMLQDLGCVAIMPLASL
                     IGSGMGILNPWNVQLVIDAVDLPVIVDAGVGTASDAAIAMELGCDGVLMNTAVAAAKD
                     PVRMARAMKQAVEAGREAYLAGRMPKKLYSAAPSSPVEGKIAPSRSKAA"
     gene            6057..6710
                     /gene="trmB"
                     /locus_tag="VFB53_06285"
     CDS             6057..6710
                     /gene="trmB"
                     /locus_tag="VFB53_06285"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZN86376.1"
                     /translation="MKRTVRSYVLRQGRVTAGQERALAELMPRYGIAFQDRTLRPAEV
                     FGREAPLVLEIGSGMGETTARIAQARPETDFIAVEVHGPGVGSLLKRIDADRLRNLRV
                     IRHDALEVLEKMIPDGALAGIHLYFPDPWPKKRHHKRRLVQPAFAALAARKLAPGGIL
                     HAATDWDDYAAQILEVLSGTPDFAREQGAAARPPTKFELRGMQLGHKVHDFLFRRRS"
     gene            6712..7653
                     /locus_tag="VFB53_06290"
     CDS             6712..7653
                     /locus_tag="VFB53_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86377.1"
                     /translation="MLRWLLLSTALVVGAHARAEPSAHDLDWTDESRQRAVPVRLYWP
                     AGAQPVPLVVFSHGIGGSRLGYSYLGEYFASQGMASLHLQHVGSDRSLWGGNPLSLVG
                     RLQDAAQEHEALERVKDLSFALDQLLTHHEFGARIDRARIAAAGHSYGANTVMLAAGA
                     SVQRAGRRLNVRDPRIKAAVLLSAPPFYGEDDARAILQSVTLPTLHVTATEDIIRIPG
                     YYSPASDRVAVYEAVGSPFKALAVFEGGSHSIFTNRGGSGGAELNPKVKSATKELAIA
                     FLQRVFEGKSEALARWPETYRALLARWTSPGTSAAAR"
     gene            complement(7622..8773)
                     /gene="hemW"
                     /locus_tag="VFB53_06295"
     CDS             complement(7622..8773)
                     /gene="hemW"
                     /locus_tag="VFB53_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZN86378.1"
                     /translation="MRAPGELRFAALPPLALYAHLPWCVRKCPYCDFNSHEKTDALPE
                     REYVDALVADLEGLLPSVWGRRLRSVFIGGGTPSLFAPGAIDRLLAAVRARVPLEPDA
                     EVTLEANPGTAEAGRFRGYRDAGVNRLSLGIQSFDDAKLKALGRIHSADEARRAIDMA
                     QAVFDNVNLDVMYGLPGQTLEEARADAAEGLRFGTTHFSAYQLTLEQNTVFYSKPPRL
                     PEHDACADMQLAVEELAAAAGFEHYETSAFARPGRRCRHNMNYWEFGDYLGIGAGAHG
                     KLSFVDRVTRHERAKQPREYMASAGTLQEKVVAPRELPFEFMLNALRLVDGVPAQLFA
                     ERTGLAGATILRRLQAARDRGLLEEDALRIRPTRRGRLFLNELLQMFLD"
     gene            complement(8782..9369)
                     /gene="rdgB"
                     /locus_tag="VFB53_06300"
     CDS             complement(8782..9369)
                     /gene="rdgB"
                     /locus_tag="VFB53_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZN86379.1"
                     /translation="MRRLVLASGNPGKLREIRALLAPLAIEVIPQESLGIAEADEPHD
                     SFLDNALAKARHASGASGLPALADDSGLCVDALGGAPGVHSAYFAGREGSRAERDARN
                     NAKLLASLGEQREAHYACVMALVRSAADAQPIVAAGTWRGEIARAARGANGFGYDPLF
                     LLPERGLTAAELGPEEKNRISHRGRAAARLLELLR"
     gene            complement(9366..10274)
                     /locus_tag="VFB53_06305"
     CDS             complement(9366..10274)
                     /locus_tag="VFB53_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HZN86380.1"
                     /translation="MKFAIAQETRVGGRPYNEDRAGHWSTGEALLLVVCDGMGGHAHG
                     EIAAELVMRHIAAAFRRDARPRLADPDLFLFRAVGRSHGMLLREAGNLGLQDTPRTTV
                     VACVVQEGRAYWSHVGDSRLYLLRKGEVLVRTRDHTLVQQLVDEGRLPAADVSSHPER
                     NRLLQCLGGVQAPRVLPTAHALLEKEDVLLLCSDGLWGPLSERQILGAFAGAPPAEAV
                     PRLAGLAELQGGPGADNITALALGWGEERRVEPAGPSTVPLAEVAAAAQDLGATDSDF
                     LRLSDDDIERAIAGIREALKRGKAPR"
     gene            complement(10271..11215)
                     /locus_tag="VFB53_06310"
     CDS             complement(10271..11215)
                     /locus_tag="VFB53_06310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN86381.1"
                     /translation="MPAQANQPLPEGHQLQNYRILRVLASGGFSFVYLAHDENEAPVA
                     IKEYLPSTLALRTGTGSVPVVPESHAAAFRYGMKCFFEEGRALATLAHPNVVRVLNFF
                     RANETVYLVMRYERGRTLQEHIHARRGALKETWIRNTFASLLNGLREVHSNKLLHLDI
                     KPGNVYLRSDGTPLLIDFGAARQMLSGEAPALPPMYTPGFASPEHHTDRAKLGPWSDV
                     YSVGATLYACLTAQAPPSAEARLEKDRMPPARKAGGGFTSELLNTVDWCLRLNHLERP
                     QSVFTLQKALLGEKAPEYQGEAPVLEQLKGALLRLAGR"
     gene            11267..12127
                     /locus_tag="VFB53_06315"
     CDS             11267..12127
                     /locus_tag="VFB53_06315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZN86382.1"
                     /translation="MIFSMTGYALAAAEHPRGALSLELKSVNSRFLDLQFRVADELRA
                     LEPVLREAITARVTRGKVDCRLSFAPGVAGAQSLDPAALEALRRIAGEAQRAFPQAGP
                     LRLGDVLRWPGVLAGAAPDAEELRGALAALAGRAVDELCAARAREGAKLAALLAGRVA
                     EMRRRLAEVAPLVPEANAAYQARLAERLREALGAADEERLRAELALFAVKTDVAEELQ
                     RLGSHLEEVERTLARGGAAGKRLDFLAQELNREANTLAAKAATQRIADCALELKLLIE
                     QLREQVQNIE"
     gene            12124..12732
                     /gene="gmk"
                     /locus_tag="VFB53_06320"
     CDS             12124..12732
                     /gene="gmk"
                     /locus_tag="VFB53_06320"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZN86383.1"
                     /translation="MTGLLYVVTAPSGAGKSSLIKALLAQDPGLVLSVSYTTRAPRPG
                     EQDGREYHFVDLPRFQAMLERGEFVESAEVHGHRYGTSQKVIEQTRAAGRDLLLEIDW
                     QGAKQVRRLHADCIGIFILPPSLAELERRLRKRAQDSEAAIRRRLENAVEEMRHAVEF
                     KYAIINNNFDDALRDIAAVVRAERLATGRQLARHPEVFKPEG"
     gene            12735..12962
                     /gene="rpoZ"
                     /locus_tag="VFB53_06325"
     CDS             12735..12962
                     /gene="rpoZ"
                     /locus_tag="VFB53_06325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZN86384.1"
                     /translation="MARITVDDCLHRIPNRFMLTLAATYRARQLGAGASPLVEPNRDK
                     STVIALREIASGKVGTEVLKRNPTAPTPQTP"
     gene            13011..15125
                     /locus_tag="VFB53_06330"
     CDS             13011..15125
                     /locus_tag="VFB53_06330"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZN86385.1"
                     /translation="MAAVQSLDQSHFSYLKKADIARLADAYRFSEAAHAGQRRQSGDP
                     YISHPLAVAEILAGWHLDGQALMAALLHDVMEDTSVTKAEISDTFGKPVADLVDGLSK
                     LDKIEFQSAEDAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLDAVPPAKRRRVAR
                     ETMEIYAPIANRLGLNALFHELQELAFAHLYSLRYRVLSKATKAARGNRREVVGKIEA
                     AIKAKLDAAGIAATVFGREKHVYSIYRKMVEKHLSFSEVHDIYGFRVIVKDVPTCYLA
                     LGALHALYKPVPGKFKDYIAIPKANGYQSLHTALIGPYGVPVEVQMRTEQMHRLAEAG
                     VASHWMYKDDGASLSDLQKKTHQWLQSLLEIQNQSGDPHEFLEHVKVDLFPDEVYVFT
                     PKGRILSLPRGATAVDFAYLVHTDIGNRCVAAKVNGELVPLRTELRNGDRVEIITASH
                     AKPNPAWLQYVRTGKARANIRHFLKTMQYDESAALGERLLEQALKGLGAGLAEIDDAN
                     WERVLRETVGRTKQELLADIGLGKQLPAVVARRLLKRVDAAQDAKGKAALVVIRGTEG
                     MAVQLASCCRPIPGDAIVGSMKKGQGLVVHSDECGNIERSRRNEPEQWIDVEWDPASS
                     RLYQSAIRVMVANQRGVLAKVASEIADSGSNIDSISMDEDRSVYTNMLLVVEVSNRRH
                     LARVMRALRRLPDVKKLARVRE"
     gene            15145..16206
                     /locus_tag="VFB53_06335"
     CDS             15145..16206
                     /locus_tag="VFB53_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86386.1"
                     /translation="MPLMRLLLAVCVAACSPGAAAQDAATPKDGSWVARDFRFHTGET
                     LPELRLHYTTLGNPASEPVLILHGTTGSGTGMLNPRFGGELFGPGQPLDAAKHYVILP
                     DSIGHGKSSKPSDGMRTRFPRYNYEDMVLAQYRLLTEGLGIRHLRLILGNSMGGMQAW
                     IWGVKYPEFMDALVPMASMPTEMASRNWMLRRLITDSIRNDPEWKNGDYTAQPRSAQF
                     ASVFFGIATNGGDQAHFRAAPTREKADALLDQRLKAPFRGDANDILYQWDSSRDFNPS
                     AALERIRAAVLAINSADDERNPPQLGVMERELKRMHNARLYLIPASDQTAGHGTTGQA
                     RWWKQELGAFLQGAPRRGK"
     gene            16196..16972
                     /gene="fabG"
                     /locus_tag="VFB53_06340"
     CDS             16196..16972
                     /gene="fabG"
                     /locus_tag="VFB53_06340"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN86387.1"
                     /translation="MASEKALARAAELAGKVALVTGGARNIGRAIACALAAGGAAVMV
                     HARSSRAEAEETAGMIRSAGGRVALHFADVTDAAAVAALVEATLAEFGRLDLLVNNAA
                     TRAESPFAEMRFDDWRRVLATILDGAFLCTQACLPHLASAGGGAVVNIGGMTAHTGAR
                     DRAHVVAAKAGLVGLTRALARDLAPQGITVNCVIPGTIETVRGLPGAPERPAHRQSLP
                     PLGRRGEPEEVAAAVRFLCGPGARYITGQSLHVNGGGFMA"
     gene            16969..18336
                     /locus_tag="VFB53_06345"
     CDS             16969..18336
                     /locus_tag="VFB53_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN86388.1"
                     /translation="MTAAAPAVAESLAHCAAALRPAQIPDEVRGRCRDLLVDVAGLCV
                     AARNRDYVAALKGSLDGGGPCTALGHARGWSPEDAALLNGTAAHGEDFDDTFEGGPVH
                     SGAVVVPAVLAACERFQRDGSDALLGIATGVETLCRLSLVAPQRIHKAGFHPTAVLGA
                     IAAALAVSRTLGASGKQTVDALGIAGSMAAGIIEYLAEGAWTKRLHAGWAAHCGLHAA
                     RLALQGFVGPRTVLEGTHGLFHAFARDSRGDYAALTRGFGDEWRMTGLTFKPYATGTM
                     NQPYVDCALRLAQRGFAAEDVAAVLCETAEGYVHRLWEPLADKQRPPNDYAAKFSTPF
                     NVAVAFVTGGAGFDAFSERTVRDARILALAGKVRYAVDPHNPYPQAYTGHVRMTLRDG
                     RVFEERQPHIRGGVHEPLAREDIERKFRGNAAYGGWAESRAAQFLAFAENAFHGTPDL
                     SPFRV"
     gene            18302..19279
                     /locus_tag="VFB53_06350"
     CDS             18302..19279
                     /locus_tag="VFB53_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86389.1"
                     /translation="MARRTCLLSAFSLLLAALLPGLAGAQAYPAKPVRIIVPAQPGGG
                     LDLVGRTLADQLGRALGQSFIVENVSGGGGTIAALTVARSAPDGYTLMVGYVGTHGTN
                     PAVRKVPYDAVGDFTPIAMVGGTANILVVNPALPVAGLKSFVEYAKGNAATLNIASGG
                     IGTLNHLAMEQFRTAAGFTGVTAHFRGIGPAITALRAGHIQVMMPGLAAALPHIRARN
                     MRAIAVTGLQRHPLVPDVPTFEESGYRGFDAVQWYGIVGPARMPGAVAQRLNGEINHA
                     LASGELRQRLSAEAIDPMPMSPQEFGKFIQADIARWRALARAQGLSLDD"
     gene            19293..20522
                     /locus_tag="VFB53_06355"
     CDS             19293..20522
                     /locus_tag="VFB53_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN86390.1"
                     /translation="MSAPAFAPFRVRSFRFQWPADLATSWAFEMEALILGWFVLSTTG
                     SVQLLVLVGALAWLGSLFSPLFGIAGDRIGVRTLLCITRGAYAMLAAVLAALTLAEAL
                     APWHVLVIAGLAGLMRPSDMAMRHVLVGQTMQPESLMGALAISRTTSDTARIAGALAG
                     TGGVALIGMGPAYVAVSAIYVAAFLLSLGVERHPPRAAGAKVDQLFAGLRLGVRYVWD
                     KPDLLGAFSMAFLVNLLAYPFFLGLLPYVARDVYGIGQSGLGYLAGAFASGALAGSLA
                     VSANRVPLPAARVMLLSGAFWFVAIILFGQIRSVGVGLALLFVTGFVQSFCLTPLAAV
                     MLRASSEDMRGRVMGIRMLAIWGLPLGLLAAGPLIERLGYSATTLLYAGLGLAATFAI
                     GMRWRRALWHHSAAANL"
     gene            complement(20534..20609)
                     /locus_tag="VFB53_06360"
     tRNA            complement(20534..20609)
                     /locus_tag="VFB53_06360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20574..20576),aa:Lys,seq:ctt)
     gene            complement(20638..20713)
                     /locus_tag="VFB53_06365"
     tRNA            complement(20638..20713)
                     /locus_tag="VFB53_06365"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20678..20680),aa:Lys,seq:ttt)
     gene            complement(20765..21436)
                     /gene="queC"
                     /locus_tag="VFB53_06370"
     CDS             complement(20765..21436)
                     /gene="queC"
                     /locus_tag="VFB53_06370"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZN86391.1"
                     /translation="MNAVVLLSGGLDSATALAWARREGYRCHALSVDYGQRHAAELGA
                     AARIALRLGAIEHRVVKLDLAQFGGSALTDRGIAIPQSGLHPGIPVTYVPARNTILLS
                     LALAWAETLQAQHIFVGANAVDYSGYPDCRPEYLKAFEAMANLATKSAVEGRSIEVRA
                     PLIDLPKKEIVRLAIELGVDPADTVTCYQADAQGRACGACDACRIRKNGFVDAGIRDS
                     TRYIA"
     gene            complement(21433..22206)
                     /gene="ybgF"
                     /locus_tag="VFB53_06375"
     CDS             complement(21433..22206)
                     /gene="ybgF"
                     /locus_tag="VFB53_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZN86392.1"
                     /translation="MRWLLLAAVLGWAGSAAAQLFGDSETKRRLDELRQEFQASQQRI
                     DARLGSLESAAVDRRVILDLAGQIEALRAELARTRGQVEVMLNQLEVAEKRQKDLYVD
                     IDTRLRRLEQVQEQASAAALKPPADAGPSDAEKKTYESALNHFKLGNYSAAIDLLQNM
                     LSSYPSSKLAPNAQYWIGMGYSGRRDYRNAIAAHQKVISTWPDDPKAPDAMLSIASAQ
                     EAMGDKNGAQKTLQNVLAKYPGSSAAANAKLRLTQSGKR"
     gene            complement(22206..22751)
                     /gene="pal"
                     /locus_tag="VFB53_06380"
     CDS             complement(22206..22751)
                     /gene="pal"
                     /locus_tag="VFB53_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZN86393.1"
                     /translation="MRTLSLAFIACTLAACTATQTPDRQPPAAVEDRAPGAVAQPAPS
                     APPAGVDLTGRPASGAAALKDPNSILSKRSVYYDFDKFNIKDDYRPMIEAHARYLREN
                     GGTKMLVQGNTDERGSREYNLALGQRRSEGVKRMLMLLGGREEQIEAVSLGEEKPRMA
                     GSNEEAWAQNRRSDMLYPGEY"
     gene            complement(22766..24046)
                     /gene="tolB"
                     /locus_tag="VFB53_06385"
     CDS             complement(22766..24046)
                     /gene="tolB"
                     /locus_tag="VFB53_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZN86394.1"
                     /translation="MRNLLAAAALSFAACSGHAQLSIEITGAGAQRIPVAIVPFVGEG
                     ALAGGANAAISAIVRADLERSGLFRGVEVPSLVPAPTEASSVNYAEWKSRLADALVLG
                     SVAARPDGRFEARFRLHDVVKQSSIGGVAYTMSREQLRATAHRIADYVYEKLTGEKGV
                     FSTRIAYVVKRGSRYELQIADADGANEQAMLVSNEPIISPAWSPNGKRIAYVSFQNKK
                     PIVYVQDVEVPKQNIVANFKGSNSAPAWSPDGTRLAVALTRDGSSQIYLINPDGGNVR
                     RLTFSGGIDTEPRYSPDGQWIYFTSDRGGSPQIYRMAAAGGDAQRVTFEGSYNVSPRI
                     SPDGRSLAYIMRNGSKYQVALLDLGTRQVQVLTDSDLDESPSFAPNGRQILLASIAGG
                     RGVLTAVSADGRVKQRLTVNAGDVREPAWGPLLQ"
     gene            complement(24062..24811)
                     /locus_tag="VFB53_06390"
     CDS             complement(24062..24811)
                     /locus_tag="VFB53_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZN86395.1"
                     /translation="MSAALAGPFLELRREEDLAPALVLAVVVHLLLAVVLTFGVRWQS
                     RAPEVVQVELWQPPPPPPAAVQPEPPKPAPVVQPKPEPRIEKPDIALKAPKPKPKPEP
                     KPEAKPKPKPAAKADDAALRRLLAEAAQREQHQFAAERERQAIKDQLTRDAANARFKG
                     VAAWVDRIRNKIRGNIFLPLDIKGNPEALFLVTLLPNGEVLQARLVISSGHAGYDEAV
                     LRAILKSSPLPKPDSPGLFERELKLTFRPMD"
     gene            complement(24808..25236)
                     /gene="tolR"
                     /locus_tag="VFB53_06395"
     CDS             complement(24808..25236)
                     /gene="tolR"
                     /locus_tag="VFB53_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HZN86396.1"
                     /translation="MPALHRKRAPMHEINIVPYVDVMLVLLVIFMVTAPLVTPGVIDL
                     PSVSKASNAPVSPLEVVIRADRSILLRSRDAKGVPGEERRVPRADLARVIKERLAKNP
                     EQPVLIAGDKDVRYETVLQVMDELHKLQVKRVGLLVKPAQ"
     gene            complement(25239..25922)
                     /gene="tolQ"
                     /locus_tag="VFB53_06400"
     CDS             complement(25239..25922)
                     /gene="tolQ"
                     /locus_tag="VFB53_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZN86397.1"
                     /translation="MNVTADLDIWTLILGASLVVKAVMLLLLAVSFLSWMFIFSKWFA
                     IRRARGQTEQFEREFWGGKDLNLIYQGAVNNRHTIGSLERIFEAGFREFAKLRNQRST
                     TDPSVMVDGARRAMRATYQRETDHLERHLAFLASTGSVSPYVGLFGTVWGIMHAFRSL
                     ANVQQATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRYSHQIDRLAIRFESFMEEFS
                     NILQRQAVK"
     gene            complement(25927..26337)
                     /gene="ybgC"
                     /locus_tag="VFB53_06405"
     CDS             complement(25927..26337)
                     /gene="ybgC"
                     /locus_tag="VFB53_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HZN86398.1"
                     /translation="MQRRDARVFSWPVRVYYEDTDFGGVVYYANYLRYFERARTEWLR
                     TLGIDHQGLAREAGLQFVVRRAEIDFLRGARLDDQLEITVELTERKRSYLMLKQRALR
                     GTEPMAEALVQAACVRQSDFRPAAIPATFAAQLG"
     gene            complement(26361..27407)
                     /gene="ruvB"
                     /locus_tag="VFB53_06410"
     CDS             complement(26361..27407)
                     /gene="ruvB"
                     /locus_tag="VFB53_06410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZN86399.1"
                     /translation="MSVETDRLIAAAPASAQEEQFERSLRPVRLADYVGQAKVRDQLG
                     IFIEAARGRSEALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAAL
                     LTNLEPRDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLPPFTLV
                     GATTRAGMLTNPLRDRFGIVARLEFYSEPELAAIVRRSAGLLAVTLATEGGAEIARRA
                     RGTPRVANRLLRRVRDYAQVKAEGAVTRAVAEAALKMLDVDALGLDVMDRKLLLAVIE
                     RFGGGPVGVENLAHAIGEESDTIEDVLEPYLIQQGYLQRTARGRVATLAAYRHFGLVA
                     PSGAHDLLGPDARG"
     gene            27477..28067
                     /locus_tag="VFB53_06415"
     CDS             27477..28067
                     /locus_tag="VFB53_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN86400.1"
                     /translation="MATIRTFVAALSAAFWLPGALAQAPADNGWYLGSGLLPVKPRLQ
                     LAGPAIPGEPREAAEPGYQQFGGFRFTRNWSLDIGYAGGNRPAAGDGSIHANAWTFSG
                     TGLLPITRSFSLQGRLGVAMPTAEFSLTSAASTLSSLAGDSARNRMNMLWGVGGQYEF
                     SPTVGLRMDYNSRYADDTGYNRARTDLWSINAVVRF"
     gene            complement(28074..28637)
                     /gene="ruvA"
                     /locus_tag="VFB53_06420"
     CDS             complement(28074..28637)
                     /gene="ruvA"
                     /locus_tag="VFB53_06420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZN86401.1"
                     /translation="MIGRLSGTLVAKHPPQVMVDCNGVAYELDVPMSTFYNLPATGQP
                     VVLLTHLVVREDVQALYGFGTLEERAAFRQLLKISGVGARTALSVLSGMSVPELAQAV
                     ALQEPARLTKVPGIGKKTAERLLLELKGKLADARPAVGGGAADVLNALLGLGYSEKEA
                     AAALQGLAPGLPVAEGIRAALKALAKS"
     gene            complement(28663..29178)
                     /gene="ruvC"
                     /locus_tag="VFB53_06425"
     CDS             complement(28663..29178)
                     /gene="ruvC"
                     /locus_tag="VFB53_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZN86402.1"
                     /translation="MRILGIDPGLQVTGFGVIEHSGRRLDYVASGCVKSGRGELAERL
                     KVLLEGLAEVIATHRPAQVVVEKVFVNVNPQSTLALGQARGTAICAAVLAGLPVAEYT
                     ALQVKQAVVGNGHAKKEQVQHMVKRLLALPGSPGADAADALACAICHAHGGRLGALPM
                     HGLRMKRGRLA"
     gene            complement(29175..29903)
                     /locus_tag="VFB53_06430"
     CDS             complement(29175..29903)
                     /locus_tag="VFB53_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN86403.1"
                     /translation="MAGHSKWANIKHRKAAADAKKGKVFTRLIKEITVAAKMGGGDPN
                     MNPRLRLAMDKGREANMAKDSVQNAIKRGTGQLEGVSYEEVRYEGYGPGGAALIVDCL
                     TDNRTRTVADVRHAFTKNGGNLGSDGSVAFMFKHCGLFMFAPGASEEKVMEAALEAGA
                     EDVVANDDGSIEVLSAPHDFHQVKAALEKAGLKPELAEVTMKPVNDSALAGGDAQRMR
                     SLLDALEVLDDVQDVYTTAAFEDA"
     gene            29920..31413
                     /locus_tag="VFB53_06435"
     CDS             29920..31413
                     /locus_tag="VFB53_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HZN86404.1"
                     /translation="MATALLVARSGKTDLGLLPALANRHGLITGATGTGKTVTLQTLA
                     EQLSRIGVPVFMADVKGDLSGMAKAGGANAKVAERAKLLKLELTYAACPLVFWDVFGE
                     SGHPVRATVSDMGPLLLARMLGLNDTQEGVLNLVFKIADDAGLLLLDTKDLRAILQFV
                     GENAAKFTTEYGNVSTASIGAIQRGLLTLETQGADRFFGEPMLNIADLMQVEGGKGVV
                     NILAADRLMNSPKLYGTFLLWMLAELFEQLPEVGDVDKPKLVFFFDEAHLLFDDAPRA
                     LLDKVEQMVRLIRSKGVGVYFVTQNPLDIPESVLGQLGNRVQHALRAFTPKDQKAVKS
                     AAETMRPNPKLRIEQAITELGVGEALVSLLDEKGRPGVTERAFVVPPGSQLGPLTDAE
                     RKAAIASSPLAGHYGKAVDRESAYEKLKARADQAAQEAAAQTTQAQPKESSVASDILF
                     GKTGPRGGRQSQGLLEAMAKSAARTVGSQVGRELIRGVLGSLLGKRR"
     gene            31410..32306
                     /locus_tag="VFB53_06440"
     CDS             31410..32306
                     /locus_tag="VFB53_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN86405.1"
                     /translation="MSPAAYRGWGLLFALAGTVAFAFRPVLIKLGYEAAPVSATTLLF
                     LRMVLSLPFFAAVAWWLRREPGLARRDWLGIVGLGFLGYYLASLLDFLGLQYVPAGHG
                     RLIMFLYPTLVVILSALFLGKRATARELAALAVTYTGIALVLSHQLGAPAENRLFFLG
                     AALVFGSAMCYAVYLVTGSQLIKRVGSMRFTAYTMMVSTVPAVIQFGVLEGPASLSLP
                     GIVWIYAIVLATVCTVLPVFLVAESLKRIGASQFALIGGLGPVTTVLADFALLEGSLS
                     VLQVLGGALVIGGVLLVTLKKG"
     gene            32312..33091
                     /locus_tag="VFB53_06445"
     CDS             32312..33091
                     /locus_tag="VFB53_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86406.1"
                     /translation="MDLGIKGRNALVCAASKGLGKGCAMALAREGVSLVITARGKEAL
                     EATAEEIRRATGAKVVAVAGDITTAEGRAQALAACPSPDILVNNAGGPPPGDFRNWTR
                     EDWIKAVDANMLTPIELIKATIDAMIAKKFGRIVNITSGAVKMPIPELGLSNGARTGL
                     TGFVAGLARTTVQHNVTVNNLLPGPFDTDRLRSNIEFNAKKLGKTPAEFAQARREANP
                     AKRFGTAEEFGDACAFLCSAQAGYITGQNLLLDGGAYPGTF"
BASE COUNT         4785 a        11879 c        11384 g         5095 t
ORIGIN      
        1 tcatgctgcg ccacgtgctg cccaacgtca ccgggccggt gctggtgatc gccaccatcc
       61 acgtcgccac cgccatcatc accgaggcga cgctgtcgtt cctcggcgtc ggcgtgccgc
      121 cgacgcagcc ttcgctcggc acgctgatcc gcatcggcaa cgatttcctc atctcgggcg
      181 agtggtggat caccgtgttt cccggcgtcg cgctggtggt gctggtgctg tcggtgaacc
      241 tgctgggcga ctggctgcgc gacgccctga acccgaagct gcggtgagcc ccgcgccgct
      301 gctcgaggtg cgcggactgc gcgtcgaatt cccgacgcgc cgcggcacgc tggtggcggt
      361 ggacgacctg tccttcgcca tcgcgcccgg cgaagtgctc ggcgtggtgg gcgaatccgg
      421 cgccggcaag tcgctcaccg gcatggcgat catcgggctg ctggagccgc cggggcgcat
      481 cgccgccggc gagatccggc tagagggcga gcgcatcgac cagctgccgc ccgagcgcat
      541 gcgcaggatc cgcggccggc gcatcggcgc gatcttccag gatccgctca cttcgctcaa
      601 cccgctgtac accgtgggcg accagctggt cgagaccatc ctcacgcacc tggaggtgga
      661 ccgggacgag gcgcgcgacc gcgccctcaa gctgctggcc gaggtcggca tcacggcgcc
      721 cgcggcgcgc atcgaccact acccgcacca gttctccggc ggcatgcgcc agcgcgtggt
      781 gatcgccctt gccctgtgcg ccgagccgag cctgatcgtc gccgacgagc cgaccaccgc
      841 gctcgacgtg tcgatccagg cccaggtcat cgcgctgttg aagcgcctgg cgcgcgagca
      901 cggcaccgcg gtgatgctga tcacgcacga catgggcgtg atcgccgaga ccgccgaccg
      961 cgtcgcggtg atgtacgcgg ggcgcctggc cgagctcggg ccggtgcgcg aggtgatcca
     1021 ccagccgcgc cacccctaca ccgccgggct gatgggctcg atccccaaga tcggcgcgtt
     1081 gaagcgccac gaccgcctgg tgcagatcga cggcgccatg ccgcgcttga acgccattcc
     1141 ggcgggctgc gccttcaatc cccgctgccc gaagcgcttc gagcgctgct tcgccgaacg
     1201 gcccgagccg ctgcaggtcg gcaacgcgct cgcgtcctgc tggctgtacg ccgatgcacg
     1261 ccgctagccg gccgctggtc cacttgaccg acgtcgggag ggatttcgac gtctcgaagc
     1321 cctggctcaa ccgcctcgtc gagggcgcgc cgcgccagct gctgcgcgcg gtggacggcg
     1381 tgagcttcga cgtgaagcgg ggcgagacgc tggcgctggt gggcgagtcg ggctgcggca
     1441 agtccaccgt cgcccgcctg atcgtcggcc tgtacgcgct gtcgcgcggc cgcatcgaat
     1501 tcgacggcat cgatctcgcc gcgccgggca ccgacgcgcc cgccctgcgc cggcgcatgc
     1561 agatgatctt ccaggacccc tatgccagcc tgaatccccg ctggcgcgtg cgcgacatcg
     1621 tcgccgagcc gatccgcgtg ctcggcctcg ccgcctcgcc gcaggccgag cgcgaccggg
     1681 tggccgagct gctgcgccag gtgggcctcg cgcccgagga cggcgagaag tacccgcacg
     1741 agttctccgg cgggcagcgc cagcgcatct cgatcgcgcg cgccctctcg ggcaacccgg
     1801 agttcctggt atgcgacgag cccacctcgg cgctcgacgt ctcggtgcag gcgcagatcc
     1861 tcaatctgat gaccgagctg caggagcgcc tcggcctgac gtacctgttc atctcccaca
     1921 acctggcggt ggtatcgcac atcgccgacc gtgtcggcgt catgtacctg gggcgcgtgg
     1981 tcgagcttgc cggggccgca gagctgttcg ccgcgccccg gcacccctat acccgcatgc
     2041 tgatggacgc gatccccgac ctgcagatga ccggcaaggc gcgcacgccg gtcgcgggag
     2101 aagtgcccaa cccgctcgcg ccgcccgccg gctgcgcctt ccatccgcgc tgcccgcacg
     2161 ccaatgcgcg ctgccgcagc gagcggcccc tgttgaccgg ctccggcgcg gccgcggtcg
     2221 cctgccacgc cgtggaagaa cggcgcctct agcgcttgag caggtcgcgc agcttgtctt
     2281 tcaccttctc cttcgccttg gccttcgccg cgccggtggc gagcgagcgg tagtcgatct
     2341 cgaccttcgg ggcatcgaac ggcccgctga gcttcaccgg cacggtgagc ccggcgaggt
     2401 gatcgaggtc cgccccgccc tgccccttcg aggtcgcgac caccgtcgcg cgggcgacat
     2461 agttcagctg cgaattgccg atgtcgatgt cgcccgcgcc gcccaggcgc accagcggcg
     2521 ccttcacgtc gaggtcctcg ttgtgcgcca cgccgccctt gatcgcgaag ctcgccgtca
     2581 tctcggagaa atcggtgcgc tgcgacgggt cggcgctctg ctgctgcgcc gacttggagc
     2641 cgaaggcggc cttggtcctg cgcagggcct cggtgaggtt gaagcccttc agcgcgccgt
     2701 cgcgcagcgc cacgcgcgcc gtgccgccga gggatttctt catggcgttg accgtcgccc
     2761 ccgccgccgc caggtccagc gtcacgtcgc cgcgcccttc caccgcgtcg cgacccatca
     2821 ggtccttgat cagcggaccg acggcgacgt tggcgagcga ctccttcatc gccacgcggt
     2881 tgccgccggc ctccagcgtc agggctccct gcagcgtgcc ctcgtagagc ttcgccgagt
     2941 gcggcgccag ctcggccctg ccgtcggcgg cgcgcagctg ggccttcagc tcggcaagct
     3001 tcaggccctg cagctgcagc tcgccgacgg cgagcctgcc ggtggcgcgc aggcccttga
     3061 gcgcacccag gtcgaccggc acatcgacgt cgaccttggc gttcgcgccg ggcgccggct
     3121 tcgccttggg gctcgccacc agatagcggt cgaggttgat ccgatcgacg tccaggtcga
     3181 agctgtagcg cggggcatcg aaccccgcca tgccggtttt ggccttgatg gtggtgtcgt
     3241 cgaacttcac cgtgaggtcg gcattcacgc tctccttcgc cacgtcggcc cgtacgtcgc
     3301 ccgacagggc gagcgccaag cggctgaaat ccgccgcggt cccctccacc ttgagcgcga
     3361 ggccgctgag cgcgtactcc tggcgcgcga agctgaggcg gtagcccgcc tccagcgcca
     3421 ccttggcgtc gaccagcggg ttcttgccct tcagcgcggc gcccaggctc atcctgccgg
     3481 gcacatcctc ggcgatgcgc ccggtgctca ggtccaggtg ggcgatctcc agggtctgcc
     3541 cgctgccttc atcgctgtag gcgagcgtgc cgtcctccag gcggatgccg gcgatgtcga
     3601 acttcacttt ccctgcctgc ggcggcgcct tggccggcgg cttcgcgccg accgcgccca
     3661 gcaggtcctc gaagtcgaac ttgccgccct tggcgcggat cacgctcgcc ttcagtcccg
     3721 cgacgcgcac ctggtccacc agcacctcgc cgcgcagcag cggcagcagg cgcaccgaga
     3781 cgtgcgccgc gtcgaaggcc gcgaagtcgt gcgcgctcgc gcgccgcgac agcgtcacct
     3841 tgcccaccgc gacgccgatc gagggccaga aggcgagctt cacgtcgcca tggaacttca
     3901 gcgtgcggcc gcttttttcc ttcaccagct tttccacttc gtccttgtac tggttcggat
     3961 cgaagctgag cgccaggatc gccgcgcccg ccaccagcag cagcagcagc ccggacaggg
     4021 tgatgagcga gaccttgagc catttcatgc gggtttcctc cccgcgacgc cgaagggcac
     4081 atggccagtg gcgacgtgct tgccggcgga ctcgacgtgc ccgcgctggt cgtcgaagta
     4141 gaagtcgggc tcgaaggcct tcaggaactc gcccttgtcc aggccgccga ggaacagcgc
     4201 ctcgtccacc gcgatcttcc agtgcatcag cgtgcgcacc gcgcgctcgt gggcgggagc
     4261 gctgcgcgcg gtgaccagcg cggtgcgcag ccgcatcggg gtgcccgcgg tctgcagccg
     4321 gtgcagcgcc tcgagcagcg gcttgaaggg cccgggcggc agcggcgtgt cgacatgcct
     4381 ggtttcgtgg cgcgtgaagg cctccagccc gtcggcctgg tagacgcgct cggcctcgtc
     4441 ggaaaacagc accgcatcgc cgtcgaaagc gatgcgcacc tcgtccaggt ggcggctcgc
     4501 cgcctgcgcc gactccggca ccacgcgcgc cgccgggatc ttcgcgtcga gcgcgcgcat
     4561 cacgtcctgc tcgttggcgg acagaaacaa attcgcgcgc agcgcgtcca ggtacttgta
     4621 gggctggcgc ccgcgcgtga acaccccgcg ctccacgcgc agcccggcgc gctgcgccga
     4681 gcggaatacc cggaggccgc tcaccgggtc gttcttggag aggatcacca cttcgacgcg
     4741 ctgcgcgtcg ggcgcattga aggcgaggag cttcttcacc aggggaaacg ccacgccctg
     4801 cttcgccggc acgtcgatgc gccccatctg cagcgcgacg taggccgcct cgccgtcccg
     4861 gtcgaagatg cggttctcct cctcgaaatc gaacagcgcc cgggaggaga tggcgaccac
     4921 gagttttccg tcgaagttga ctggcatggc gctggagcgg gtgaagggaa tcgaaccctc
     4981 gtatgcagct tgggaagttg ccgttctgcc attgaactac acccgcgggt tgaacgcgag
     5041 tgtatcattc ccccttatga ttcaagtgat tgtgaatggt tccgcgcacc gcctggagcg
     5101 gccgctcgac gtcgcggcgc tgatcgcccg cctggagctc tccggcaagc gcatcgcggt
     5161 ggaaaggaac ggcgagatcg tgccgaagag cgcgcacggc tcgactctag tcgccgacgg
     5221 cgaccgcctc gaggtggtgg tcgcggtggg gggcggatga gcgaggcctt cgtcatcgcc
     5281 ggcaaggcgt acacctcgcg cctgctgatc ggcaccggca agtaccggga tttcgcgcag
     5341 acgcgcgcgg cggtggaagc ctcgggcgcg gaaatcgtca cggtcgccat ccgccgcacc
     5401 aacatcgggc agagcgcggg cgagccgagc ctgctcgagg cgctgccgcc ctcgaagtac
     5461 acctacctgc cgaacaccgc cggctgctac agcgcggaag acgccgtgcg cacgctgcgc
     5521 ctggcacgcg agctgctcga cgggcacgac ctggtgaagc tcgaagtcct gggcgacccg
     5581 cacacgctgt atccgaacat gcccgagacg ctcgcagcgg cgaagcagct ggtgcgggag
     5641 ggcttcaagg tgatggtgta ctgctccgac gacccgatcc aggcgaagat gctccaggac
     5701 ctgggctgcg tcgccatcat gccgcttgcc tcgctcatcg gctccgggat ggggatcctc
     5761 aacccctgga acgtgcagct ggtgatcgac gctgtggacc tcccggtgat cgtggacgcg
     5821 ggcgtgggca cggcctccga cgcggcgatc gccatggagc tgggctgcga cggcgtgctg
     5881 atgaataccg cggtggcggc ggccaaggac ccggtgcgca tggcccgggc gatgaagcag
     5941 gcggtggaag cgggccgcga agcctacctc gccggccgca tgccgaagaa gctctacagc
     6001 gcggcgccct cctccccggt cgaaggcaag atcgcgcctt cccgatccaa agccgcgtga
     6061 agcgcacggt ccgcagttac gtgctccgcc aggggcgcgt gaccgccggg caggagcgcg
     6121 ccctcgccga actgatgccg cgctacggca tcgcgttcca ggaccggacg ctgcggcccg
     6181 ccgaggtttt cggccgcgag gcgccgctgg tgctggagat cggctcgggc atgggggaga
     6241 ccacggcgcg catcgcgcag gcgcggccgg agaccgattt catcgccgtg gaagtgcacg
     6301 gtcctggcgt gggcagcctg ctgaagcgca tcgacgccga ccggctgagg aacctgcgcg
     6361 tcatccggca cgacgcgctg gaagtcctgg agaagatgat ccccgacggc gcgctggccg
     6421 gaatccacct ctacttcccc gatccctggc cgaagaagcg ccaccacaaa cgccgcctgg
     6481 tgcaacccgc attcgccgcg ctcgccgcgc gcaagctcgc ccccgggggc atcctgcatg
     6541 ccgccaccga ctgggacgac tatgcggccc agatcctcga ggtgctgtcg ggaacgcccg
     6601 acttcgcgcg cgagcagggc gccgccgcgc gcccgccgac caaatttgaa cttcgcggca
     6661 tgcagcttgg tcacaaggtg cacgatttcc tttttcgcag gcgttcctga gatgctgcgc
     6721 tggctgttgc tgtccactgc cctggtcgtc ggcgcgcacg cccgcgccga gccctccgcc
     6781 cacgacctgg attggacgga cgaatcgcgc cagcgggcgg tgccggtccg cctgtactgg
     6841 cccgctggcg cccagccggt gcccctggtc gtgttctcgc acggcatcgg cggctcccgg
     6901 ctcggctaca gctatctcgg cgagtacttc gccagccagg gcatggcgag cctgcacctg
     6961 cagcacgtgg gcagcgatcg cagtctctgg ggcggcaacc ccttgtcgct cgtcggccgg
     7021 ctgcaggacg cggcgcagga gcacgaagcc ctggagcgcg tgaaggactt gagcttcgcg
     7081 ctcgaccagc tgctcacgca tcacgagttc ggcgctcgca tcgaccgcgc gcgcatcgcc
     7141 gcggcgggac actcctacgg cgccaatacg gtgatgctgg cggccggcgc gagcgtgcag
     7201 cgcgcgggcc gcaggctgaa cgtgcgcgac ccgaggatca aggcggcggt gctgctctcg
     7261 gcgccgccat tctacggcga ggacgacgcg cgggcgatcc tgcaatcggt aacgctgccg
     7321 accctgcacg tcacggcgac cgaggacatc atccgcatcc ccggctacta ctccccggcc
     7381 agcgaccgcg tcgcggtgta cgaggccgtc ggcagcccgt tcaaggcgct ggcggtgttc
     7441 gagggcggct cgcacagcat tttcaccaac cgcggcggca gcggcggggc ggagctcaat
     7501 ccgaaggtga aatcggcgac caaggaactg gccatcgcgt tcctgcagcg cgtgttcgaa
     7561 ggcaagagcg aggcgctcgc gcgctggccg gaaacctacc gcgcgctgct cgcgcgctgg
     7621 actagtccag gaacatctgc agcagctcgt tgaggaacag ccgtccgcgc cgtgtcgggc
     7681 ggatgcgcag ggcgtcttcc tccagcaatc ccctgtcgcg cgctgcctgc agcctgcgca
     7741 ggatcgtcgc gccggccaag ccggtgcgct cggcgaacaa ctgcgccggc accccgtcca
     7801 ccaggcgcag cgcgttcagc atgaactcga aaggcaactc gcgcggcgcg accactttct
     7861 cctgcagcgt ccccgccgac gccatgtact cgcgcggctg cttggcgcgc tcgtgacgcg
     7921 tcacgcggtc gacgaaggag agcttgccgt gcgcgccggc gccgatgcca aggtagtccc
     7981 cgaactccca gtagttcatg ttgtgccggc agcgccggcc gggccgggcg aaggccgaag
     8041 tctcgtaatg ctcgaagccg gcggcggcgg cgagctcctc gaccgcgagc tgcatgtccg
     8101 cgcaggcatc gtgctcgggc agcctgggcg gcttgctgta gaaaacggta ttctgctcca
     8161 gcgtcagctg gtaggccgaa aagtgcgtgg tgccgaaacg cagtccttct gcggcatccg
     8221 cacgcgcctc ctccaacgtc tgtccgggca acccgtacat cacatccaga ttgacgttgt
     8281 cgaacaccgc ctgcgccatg tcgatcgcgc ggcgcgcctc gtccgccgag tggatccgcc
     8341 ccagggcttt cagtttcgcg tcgtcgaaac tctggatgcc cagcgacagc cggttcacgc
     8401 ccgcgtcgcg gtagccgcgg aagcgcccgg cctccgccgt gcccgggttc gcctccagcg
     8461 tcacttccgc atcgggctcg agcggcacgc gcgcgcgcac cgcggccagc agccggtcga
     8521 tggcgccggg ggcgaacagg cttggcgtgc cgccgccgat gaacaccgag cgcaggcgcc
     8581 tcccccatac cgagggcagc agcccctcca ggtccgccac cagcgcgtcc acgtactcgc
     8641 gctcgggcag cgcatccgtc ttctcgtgtg agttgaagtc gcagtagggg catttgcgca
     8701 cgcaccaggg caggtgcgcg tacagcgcga gcggcggcag cgcggcaaag cgcagctcgc
     8761 ccggggcgcg catcacggcg ctcaccgcag cagctcgagc agcctggccg ccgcgcgtcc
     8821 gcggtggctg atgcggttct tctcctccgg cccgagctcc gcggccgtca ggccgcgctc
     8881 cggcagcagg aacagcgggt cgtagccgaa gccgttggcg ccgcgcgcgg cacgcgcgat
     8941 ctcgccgcgc caggtgcctg cggcgacgat cggctgcgcg tccgcggcgc tgcgcaccag
     9001 cgccatcacg caggcgtagt gcgcctcgcg ctgctcgccc aggctggcga gcagcttggc
     9061 attgttgcgc gcatcgcgct ccgcgcggct gccctcgcgc ccggcgaagt aggccgaatg
     9121 cacgccgggc gcgccgccga gcgcgtccac gcacaggccc gaatcgtcgg cgagcgccgg
     9181 cagtccgctc gcgccgctcg cgtggcgcgc cttggccagc gcgttgtcca gaaagctgtc
     9241 gtgcggctcg tcggcctcgg cgatgccgag actctcctgc gggatcacct cgatcgccag
     9301 cggcgcgagc agcgccctga tctcgcgcag cttgcccggg ttgcccgacg ccagcaccag
     9361 ccgtctcatc gcggcgcctt gccgcgcttc aaggcttccc tgatcccggc gatggcgcgc
     9421 tcgatgtcgt cgtcgctgag gcgcaggaag tccgagtcgg tggcgccgag gtcctgcgcc
     9481 gccgcggcga cttcggcgag cggcacggtg ctcggcccgg ccggctcgac gcggcgctcc
     9541 tcgccccagc ccagcgcaag cgccgtgatg ttgtccgcgc ccggaccgcc ctgcaattcg
     9601 gcgaggccgg cgagccgcgg caccgcctcg gccggcggcg cgcccgcgaa tgccccgagg
     9661 atctggcgtt cgctcaaggg cccccacagt ccgtccgagc acagcagcag cacgtcttcc
     9721 ttttccagca gcgcgtgcgc ggtgggcagg acgcgcggcg cctgcacccc gcccaggcat
     9781 tgcagcagac ggttgcgctc cgggtgcgag ctcacgtcgg cggccggcag cctgccttcg
     9841 tccaccagct gctgcaccag cgtatggtcc cgcgtgcgca ccagcacctc gcccttgcgc
     9901 aggaggtaca ggcgcgagtc gccgacgtga gaccagtagg cgcgcccttc ttgcaccacg
     9961 caggcgacca ccgtggtgcg cggcgtgtcc tgcagcccga ggtttcccgc ttcccgcagc
    10021 agcatgccgt gcgagcgccc caccgcgcgg aacaggaaca ggtcggggtc ggcaagacgc
    10081 gggcgcgcgt cgcgccggaa ggcggcggcg atgtggcgca tgacgagttc cgccgcgatc
    10141 tcgccgtgcg cgtggccgcc catgccgtcg cacaccacca gcagcagggc ctcgccggtg
    10201 ctccagtggc cggcgcggtc ctcgttgtag ggccgcccgc cgacgcgcgt ttcctgggcg
    10261 atggcgaatt tcatcggccg gccagcctca gcagggcgcc cttgagctgc tccagcaccg
    10321 gcgcctcgcc ctggtactcg ggcgccttct cgccgagcag ggccttttgc agcgtgaaca
    10381 cgctctgcgg ccgctccagg tgattgaggc gcaggcacca gtccacggtg ttgagcagct
    10441 cgctggtgaa gccgccgccg gccttgcgcg ccggcggcat gcggtccttc tccaggcgcg
    10501 cttccgccga gggcggcgcc tgcgccgtga ggcaggcgta cagcgtcgcg cccaccgagt
    10561 acacgtcgct ccacggcccg agcttggcgc ggtcggtgtg gtgctccggc gaggcgaagc
    10621 ccggcgtgta catcggcggc agcgccggcg cctcgcccga gagcatctgg cgcgcggcgc
    10681 cgaaatcgat cagcagcggc gtgccgtcgc tcctcaggta gacgttgccc ggcttgatgt
    10741 ccaggtgcaa tagcttgttg gagtgcactt cgcgcaagcc gttcagcagg ctggcgaagg
    10801 tgttgcggat ccaggtttcc ttcagcgcgc cgcggcgcgc gtggatatgc tcctgcagcg
    10861 tgcgcccgcg ctcgtagcgc atcaccaggt acaccgtttc gttggcgcgg aagaaattca
    10921 gcacgcgcac cacgttgggg tgcgccagcg tggcgagggc gcgcccctcc tcgaagaagc
    10981 atttcatgcc gtagcggaac gcggcggcgt gcgactccgg aaccaccggc acgctgccgg
    11041 tgccggtgcg cagcgccagc gtcgagggca ggtactcctt gatcgccacc ggcgcctcgt
    11101 tctcgtcgtg cgccaggtag acgaaggaaa agccgcccga ggccagcacg cgcaggatgc
    11161 ggtagttctg cagctgatgc ccttccggca gcggctggtt ggcttgggcc ggcatctccc
    11221 ctatgatacg cgcttcgcat cgcgcacttt cctgcgttcc cctcccttga tcttcagcat
    11281 gaccggatac gcgcttgccg ccgccgagca cccgcgcggc gcactgagcc tcgagctgaa
    11341 gtcggtcaat tcgcgcttcc tcgacctgca gttccgcgtc gccgacgagc tgcgcgcgct
    11401 ggagcccgtg ctgcgcgagg cgatcaccgc gcgcgtgacg cgcggcaagg tcgattgccg
    11461 gctgtcgttc gcaccgggcg tcgccggcgc gcagtcgctc gacccggcgg cgctcgaggc
    11521 gctgcgccgc atcgccggcg aggcgcagcg cgccttcccg caggccggtc cgctgcgcct
    11581 gggcgacgtg ctgcgctggc ccggcgtgct cgccggcgcg gcgcccgacg ccgaggagtt
    11641 gcggggcgcg ctagccgcgc tcgccgggcg cgcggtggac gagctgtgcg cggcgcgcgc
    11701 gcgcgagggc gccaagctcg cggcgctgct tgccggccgc gtcgccgaga tgcgccggcg
    11761 cctcgccgaa gtggcgccgc tggtgccgga agccaacgcc gcctaccagg cgcggctcgc
    11821 cgagcggctg cgcgaagcgc tgggcgcggc ggacgaggag cgcctgcgcg ccgagctggc
    11881 gctgttcgcg gtgaagaccg acgtggcgga ggagctgcag cgcctgggct cgcacctcga
    11941 ggaagtggag cgcacgctgg ccaggggcgg ggccgccggc aagcggctcg atttcctcgc
    12001 ccaggagctc aaccgcgaag ccaacacgct cgccgccaag gcggcgacgc agagaatcgc
    12061 cgactgcgcc ctcgagctga agctgctcat cgagcagctg cgcgagcagg tgcagaacat
    12121 cgaatgacag gcctgctcta cgtggtcacc gcgccttcgg gcgcgggcaa gtcctcgctc
    12181 atcaaggcgc tgctggcgca ggacccgggc cttgtgctgt cggtgtccta caccacgcgc
    12241 gcgccgcgcc ccggcgagca ggacggccgg gagtaccact tcgtcgacct gccccgcttc
    12301 caggccatgc tcgagcgcgg cgagttcgtg gagagcgcgg aggtgcacgg ccaccgctac
    12361 ggcacctcgc agaaggtgat cgaacaaacg cgcgccgccg gcagggacct gctgctggag
    12421 atcgactggc agggcgccaa gcaggtgcgg cggctgcacg ccgattgcat cggcattttc
    12481 atcctgccac cgtcgctggc cgagctggag cggcgcctgc gcaagcgcgc gcaggattcc
    12541 gaggcggcca tccgccgccg tctggagaat gcggtcgagg aaatgaggca cgccgtggag
    12601 ttcaaatacg ctataattaa caataacttc gacgacgcgc tgcgggacat tgcggcggtc
    12661 gtgcgcgccg agcgcctggc caccggccgg cagctggcgc ggcaccccga agtcttcaaa
    12721 ccggaaggtt gaccatggcc cgcatcaccg tagacgactg cctgcaccgc atccccaatc
    12781 gcttcatgct cacgctcgcc gcgacctacc gtgcgcgcca gctcggcgcc ggcgcctcgc
    12841 cgctggtcga gcccaaccgc gacaagtcca ccgtgatcgc cctgcgcgag atcgccagcg
    12901 gcaaggtggg caccgaagtg ctcaagcgca acccgacagc gcctacgccc cagaccccct
    12961 gagcagcgaa gtgccccccg aggggggcgg gcctgcggta acctttccgc atggccgccg
    13021 tacagagcct cgaccagagc cacttcagct acctgaagaa ggcggatatc gcgcgcctgg
    13081 cggacgccta ccgcttctcc gaggccgcgc acgccgggca gaggcgccag tcgggcgacc
    13141 cctacatctc gcatccgctg gcggtggccg agatcctcgc cggctggcac ctggacggcc
    13201 aggcgctgat ggccgcgctg ctgcacgacg tgatggagga cacctcggtc accaaggccg
    13261 agatctccga caccttcggc aagccggtgg ccgatctggt cgacggcctt tccaagctcg
    13321 acaagatcga attccagtcg gccgaggacg cgcaggccga aaacttccgc aagatgctgc
    13381 tggcgatggc gcgcgacgtg cgcgtgatcc tgatcaagct cgccgaccgg ctgcacaaca
    13441 tgcgcaccct cgacgcggtg ccgccggcca agcgccggcg cgtggcgcgc gagaccatgg
    13501 agatctacgc gccgatcgcc aaccgcctgg ggctgaacgc gctgttccac gagctgcagg
    13561 agctggcctt cgcgcacctg tactccctgc gctaccgcgt gctctccaag gccaccaagg
    13621 cggcgcgcgg caaccggcgc gaggtggtgg gcaagatcga ggcggcgatc aaggccaagc
    13681 tcgacgcggc cggcatcgcc gccaccgtgt tcgggcgcga aaagcacgtg tattcgatct
    13741 accgcaagat ggtcgagaag cacctgtcgt tttccgaggt gcacgacatc tacggcttcc
    13801 gcgtcatcgt caaggacgtg cccacctgct atctggcgct cggcgcgctg catgcgctgt
    13861 acaagccggt gccgggcaaa ttcaaggact acatcgcgat cccgaaggcc aacggctacc
    13921 agtcgctgca cacggcgctg atcggcccgt acggcgtgcc ggtggaggtg cagatgcgca
    13981 ccgagcagat gcatcgcctg gccgaggccg gcgtcgcctc gcactggatg tacaaggacg
    14041 acggcgcctc gctgtccgac ctgcagaaga agacccacca gtggctgcag tcgctgctgg
    14101 agatccagaa ccagtccggc gacccgcacg aattcctcga gcacgtgaag gtggacctgt
    14161 tcccggacga ggtctacgtg ttcaccccga aaggcaggat cctgtcgctg ccgcgcggcg
    14221 ccaccgcggt ggacttcgcc tacctggtgc ataccgacat cggcaaccgc tgcgtcgccg
    14281 cgaaggtgaa cggcgagctg gtgccgctgc gcaccgagct gaggaacggc gaccgcgtcg
    14341 agatcatcac cgccagccac gccaagccca acccggcctg gctgcagtac gtgcgcaccg
    14401 gcaaggcacg cgccaacatc cgccacttcc tcaagaccat gcagtacgac gagtccgcgg
    14461 cgctcggcga gcggctgctg gagcaggcgc tgaagggcct gggcgccggg ctcgccgaga
    14521 tcgacgacgc caactgggag cgcgtgctgc gcgagaccgt cggccgcacc aagcaggaac
    14581 tgctcgccga catcggcctg ggcaagcagc tgcccgcggt ggtcgcccgg cgcctgctga
    14641 agcgggtgga tgccgcgcag gacgcgaaag gcaaggccgc cctggtggtg atccgcggca
    14701 ccgagggcat ggcggtacag ctcgccagct gctgccggcc gatacccggc gacgcgatcg
    14761 tcgggtcgat gaagaagggc cagggcctgg tggtgcacag cgacgagtgc ggcaacatcg
    14821 agcgctccag gcgcaacgag cccgaacagt ggatcgacgt ggagtgggac ccggcctcgt
    14881 cgcgcctgta ccagtcggcg atccgcgtga tggtcgccaa ccagcgcggg gtgctggcga
    14941 aagtcgcctc cgagatcgcc gattccggat cgaacatcga ttccatcagc atggacgaag
    15001 accgctcggt ctacaccaac atgctgctgg tggtggaggt gtccaaccgc cggcacctgg
    15061 cgcgcgtgat gcgcgccctg cgccggctgc cggacgtgaa gaagctcgcg cgcgtgcgcg
    15121 agtaatccag gggaggagca tgcaatgccg ttgatgcgac tgctgttggc tgtttgcgtt
    15181 gccgcgtgct cgccgggcgc cgcggcccag gacgcggcga cgccgaagga cgggagttgg
    15241 gtcgcccgcg acttccgttt ccacaccggc gaaacgctgc ccgagctgcg cctgcactac
    15301 acaaccctgg gcaatcctgc gtccgagccg gtgctgatcc tgcacggcac cacgggctcg
    15361 ggcaccggca tgctcaaccc caggttcggc ggcgagctgt ttggccccgg ccagcccctg
    15421 gacgcggcga agcactacgt catccttccc gactcgatcg gccacggcaa atcgagcaag
    15481 ccctcggacg gcatgcgcac caggttcccg cgctacaact acgaggacat ggtgctcgcc
    15541 cagtaccggc tgctgacgga aggactcggc atcaggcacc tgcggctgat cctcggcaat
    15601 tccatgggcg gcatgcaggc ctggatctgg ggcgtgaagt atccggagtt catggacgcc
    15661 ctggtgccca tggcgtcgat gccgaccgag atggcgagcc gcaactggat gctgcggcgc
    15721 ctgatcaccg attccatccg caacgacccg gaatggaaga acggcgacta cacggcgcag
    15781 ccgcgcagcg cgcaattcgc ctcggttttc ttcggcatcg ccaccaacgg cggcgaccag
    15841 gcgcatttca gggcggcgcc gacgcgcgag aaggccgatg cgctgctcga ccagcggctc
    15901 aaggcgccgt tccgcggcga cgccaacgac atcctttacc agtgggattc gtcgcgcgac
    15961 ttcaatcctt ctgcggcgct cgagcgcatc cgggcggcgg tactggcgat caattcggcg
    16021 gacgacgaac gcaacccgcc gcaactcggc gtcatggagc gcgagctgaa gcgcatgcac
    16081 aacgcgcgcc tgtacctgat tccggcctcc gaccagaccg cggggcacgg cacgaccggc
    16141 caggcgcggt ggtggaagca ggagctgggc gcgttcctgc aaggcgcgcc gcggcgtggc
    16201 aagtgagaaa gcgctagcgc gggcggcgga gctcgcgggc aaggtcgccc tggtcaccgg
    16261 cggcgcgcgc aacatcggac gtgccatcgc ctgcgcgctg gcagcgggcg gcgccgccgt
    16321 gatggtgcac gcgcgcagct cgcgcgccga ggcggaggaa accgcaggca tgatccgttc
    16381 cgcgggcggg cgggtggcgc tgcatttcgc cgacgtcacc gacgccgccg cggtcgcggc
    16441 gctggtcgaa gccacgctgg cggagttcgg ccggctggac ctgctggtca acaatgcggc
    16501 gacgcgcgcc gagtccccgt ttgccgagat gcggttcgac gactggcggc gcgtgctggc
    16561 cacgatcctc gacggcgcgt tcctctgcac gcaggcctgc ctgccgcacc tggcgagcgc
    16621 cggcggcggc gcggtggtca acatcggcgg gatgacggcc cacaccggcg cccgggaccg
    16681 cgcgcacgtc gtcgctgcga aagccgggct ggtggggctg acgcgcgccc tggcccgcga
    16741 tcttgcaccc cagggcatca ccgtgaattg cgtgatcccc ggcacgatcg agacggtgcg
    16801 cggcctgccc ggcgcgcccg agcgtccggc ccaccgccag tccctgccgc cgctcggccg
    16861 gcgcggcgag ccggaagaag tcgccgccgc cgtgcgcttt ctctgcggac ccggcgcgcg
    16921 ctacatcacg ggtcagtcgc tgcacgtcaa tggcggcgga ttcatggcat gacggcggcc
    16981 gctcccgccg ttgcggagtc gctggcgcat tgcgccgccg cgctgcgacc cgcgcaaatt
    17041 cccgacgagg tacgcggccg ctgccgggac ctgctggtcg acgtcgccgg cttgtgcgtc
    17101 gccgcgcgca accgggacta cgtggccgcg ctgaaaggca gcttggacgg cggcggcccg
    17161 tgcacggcgc tggggcatgc ccggggctgg agccccgagg atgccgccct gctgaacggc
    17221 accgccgcgc acggcgagga cttcgacgac accttcgaag gcgggccggt gcattcgggc
    17281 gcggtcgtcg tgccggcggt gctcgccgcc tgcgaacgct tccagcggga cggcagcgac
    17341 gcgctgctgg gcatcgccac cggcgtcgag accctgtgcc gcctgagcct ggtggcaccg
    17401 cagcgcatcc acaaggccgg gttccatccc accgccgtcc tcggcgcgat cgccgcggcg
    17461 ctcgcggtgt cgcggaccct gggcgcgagc ggaaagcaga ccgtcgacgc cctcggcatc
    17521 gccggcagca tggccgcagg catcatcgag taccttgccg agggcgcctg gaccaagcgc
    17581 ctgcacgcgg gctgggcagc gcattgcggg ctgcatgccg ctcggctcgc actgcaaggg
    17641 ttcgtcggcc cgcgcaccgt gctcgagggt acccatggac tgttccatgc cttcgcgcgg
    17701 gacagccggg gcgattacgc ggcgctcacc cgcggtttcg gcgacgaatg gcgcatgacg
    17761 ggcctgacgt tcaaacccta tgccaccggc accatgaacc agccctacgt cgactgcgcg
    17821 ctgcgcctgg cgcagcgggg cttcgccgcg gaggatgtcg ccgcggtgct gtgcgaaacc
    17881 gccgagggct atgtgcaccg cctctgggag ccgctcgccg acaagcagcg cccgcccaat
    17941 gactacgccg caaagttcag cacgccgttc aacgtcgccg tcgcctttgt caccggcggc
    18001 gcggggttcg acgccttcag cgagcgcacc gtacgcgacg cgcgcatcct cgcgctggcc
    18061 ggcaaggtgc gctacgcggt cgatccgcac aatccctacc cgcaggccta caccggccac
    18121 gtgcggatga ccctcaggga cgggcgtgtc ttcgaggagc gccagcccca tatccgcggc
    18181 ggcgtgcacg agcccctcgc gcgcgaggac atcgagcgga aattccgcgg caacgccgcc
    18241 tacggcggct gggcggagtc tcgcgccgcg caatttctcg ctttcgccga aaacgccttt
    18301 catggcacgc cggacctgtc tcctttccgc gtttagcctc ctgctcgccg cgctgctgcc
    18361 cggcttggcc ggcgcgcaag cctatcccgc caagcccgtg cgaatcatcg tgcccgcgca
    18421 gccgggcggc ggcctcgacc tggtcgggcg caccctggcg gaccagctcg gccgcgcgct
    18481 gggccaatcg ttcatcgtcg agaacgtctc gggcgggggc ggcaccatcg ccgcgctgac
    18541 ggtggcgcgc tcggcacccg atggctacac cctgatggtc ggctacgtcg gcacccatgg
    18601 caccaacccg gccgtgcgca aagtccccta cgatgccgtc ggggacttca ccccgatcgc
    18661 gatggtgggc ggcaccgcca acatcctggt ggtgaatccc gcgctcccgg tggccggctt
    18721 gaagagcttt gtcgagtacg cgaagggcaa cgccgcgaca ttgaacatcg cctccggcgg
    18781 catcggcacg ctcaaccacc tcgcgatgga gcagttcagg acggcggccg gcttcaccgg
    18841 cgtcacggcg cattttcgcg gcatcgggcc tgcgatcacc gccctgcgcg cgggccacat
    18901 ccaggtcatg atgcccgggc tggccgccgc cctgccgcac atccgcgcgc ggaacatgcg
    18961 ggcgatcgcc gtcaccgggc tgcagcgcca cccgctggtg cccgacgttc ccacgttcga
    19021 ggagtcgggc taccggggat tcgacgcggt gcagtggtac ggcatcgtcg gcccggcgcg
    19081 catgcccggc gctgtcgcgc agcggctcaa cggcgaaatc aaccatgcgc tggcctcggg
    19141 cgagctgcgg caacgcctct ccgcggaagc catcgatccg atgcccatgt cgccgcagga
    19201 attcggcaag ttcatccagg cagacatcgc ccgctggcgc gcactcgccc gcgcgcaggg
    19261 cctgtcgctg gacgactaga gcccgcagcc cggtgtcagc gcccgccttc gcgccttttc
    19321 gcgtccgcag cttccgtttc cagtggccgg ccgatctcgc cacctcgtgg gccttcgaga
    19381 tggaggcgct gatcctcggc tggttcgtgc tctccaccac gggctcggtg cagctgctgg
    19441 tactcgtcgg cgccctggcc tggctcgggt cgctgttttc gccgctcttc ggcatcgcgg
    19501 gagaccgcat cggcgtgcgc acgctgctgt gcatcacgcg cggcgcctac gccatgctcg
    19561 cggccgtgct ggcggcgctg acgctcgccg aggcgctcgc gccatggcac gtgctggtga
    19621 tcgcgggcct cgccggcctg atgcggccct ccgacatggc gatgcggcac gtcctcgtcg
    19681 gccagaccat gcagcccgag tccctgatgg gggcgctcgc catctcgcgc accacgtccg
    19741 acacggcgcg gatcgcgggc gcgctcgccg gcacgggcgg cgtggcgctg atcggcatgg
    19801 gcccggccta tgtggcggtg agcgcgatct atgtggctgc cttcctgctg tcgctcggcg
    19861 tcgagcgcca tccgccgcgc gccgcgggcg ccaaggtgga ccagctgttc gccggcctgc
    19921 ggctgggcgt gcgctatgtc tgggacaagc ccgatctgct cggcgccttc agcatggcct
    19981 tcctggtcaa cctgctcgcc tatccgttct tcctcggcct gctgccctat gtcgcacgcg
    20041 acgtctacgg catcggccag tcgggcttgg gctacctggc cggggccttc gccagcgggg
    20101 ccctggccgg ctcgctggcg gtgagcgcga accgagtgcc gctgcctgcc gcccgcgtca
    20161 tgctcctcag cggcgcgttc tggttcgtgg cgatcatcct gttcgggcag atccgctccg
    20221 tcggcgtggg cctcgcgctg ctctttgtca ccgggttcgt gcagagcttc tgcctcacgc
    20281 cgctcgccgc ggtcatgctg cgcgcctcga gcgaggacat gcgcggccgc gtcatgggca
    20341 ttcgcatgct cgcgatctgg ggattgccgc tcgggctgct cgcggccggg ccgctcatcg
    20401 agcgcctggg ctattccgcc accaccctgc tctatgccgg tctcggcctg gcggccacct
    20461 tcgccatcgg catgcgctgg cggcgcgcgc tctggcacca ctcggcggcg gccaatctct
    20521 aggcgatgga atctggtggg gacaccaggg ctcgaacctg ggacctactg attaagagtc
    20581 agctgctcta ccaactgagc tatgtccccc gcggacagcg tcttcccgcg cggagcttgg
    20641 taggccgtgg cggattcgaa ccgccgacca acggattaaa agtccgctgc tctaccagct
    20701 gagctaacgg cccccgggga agggcgcgat tttatcagcc ctgcaccccg gttcgccaga
    20761 aaaatcaggc gatgtagcgc gtcgagtccc ggattcccgc gtccacgaac ccgttcttcc
    20821 gaatgcggca cgcatcgcac gcaccgcagg ccctgccttg cgcatccgcc tggtagcagg
    20881 tcaccgtgtc ggcgggatcc acgcccagct cgatcgccag ccgcacgatc tccttcttcg
    20941 gcagatcgat gagcggggcg cgcacctcga tactgcggcc ctccaccgcc gacttcgtcg
    21001 ccaggttcgc catcgcctcg aacgccttca ggtactccgg ccggcaatcg gggtagccgg
    21061 aatagtccac cgcgttcgcg ccgacgaaga tgtgctgcgc ctgcagcgtt tcggcccagg
    21121 ccagggcgag cgacagcagg atggtgttgc gggcgggcac gtaggtcacc ggaatgccgg
    21181 ggtgcaggcc ggactgcgga atcgcgatgc cgcggtcggt cagggcggag ccgccgaact
    21241 gcgccaggtc gagcttgacc acacgatgct cgattgcgcc cagccgcagc gcaatgcgcg
    21301 ccgcggcgcc gagctccgcc gcgtgccgct ggccatagtc caccgacagc gcatggcagc
    21361 ggtacccttc gcgccgcgcc caggcgagcg cggtggccga atccagtccg ccggagagaa
    21421 ggacgacggc gttcagcgct tgccggactg ggtgaggcgc agcttggcgt tggcggccgc
    21481 cgagctgccc ggatatttcg ccagcacgtt ctgcagcgtc ttttgcgccc cgttcttgtc
    21541 gcccatcgct tcctgggcgc tggcgatgga aagcatcgcg tcgggcgcct tggggtcgtc
    21601 gggccaggtg ctgatcacct tctggtgcgc ggcgatcgcg ttgcgatagt cgcgccgccc
    21661 cgagtagccc atgccgatcc agtactgggc gttgggcgcg agcttgctgc tgggatagct
    21721 ggacagcatg ttctgcagca ggtcgatggc ggcggagtag ttgccgagct tgaaatggtt
    21781 gagcgcgctc tcgtaggtct tcttctccgc atccgagggc cccgcgtcgg ccgggggctt
    21841 gagcgcggcg gcggaggcct gctcctgcac ctgctcgagc ctgcgcaggc gcgtgtcgat
    21901 gtccacgtac aggtccttct gccgcttctc ggcgacctcg agctggttga gcatcacctc
    21961 gacctgcccg cgggtgcgcg cgagctccgc gcgcagcgcc tcgatctgcc cggccaggtc
    22021 gaggatcacg cgccggtcga ccgccgcgga ctccaggctg ccgaggcgcg cgtcgatgcg
    22081 ctgctggctc gcctggaact cctggcgcag ctcgtcgagc cggcgcttgg tctccgaatc
    22141 gccgaacagc tgcgccgcgg cgctccccgc ccagcccagc accgccgcga gcagcagcca
    22201 gcgcatcagt actcgcccgg atacagcatg tcgctgcggc ggttctgcgc ccaggcctct
    22261 tcattcgaac cggccatgcg cggcttctcc tcgcccaggc tcaccgcttc gatctgttcc
    22321 tcgcggccgc ccagcagcat gagcatgcgc ttcacgccct ccgagcgccg ctggccgagc
    22381 gcgaggttgt attcgcggct gccgcgctcg tcggtgttgc cctgcaccag cattttcgtg
    22441 ccgccgttct cgcgcaggta cctggcatgc gcctcgatca tcggccggta atcgtccttg
    22501 atgttgaact tgtcgaagtc gtaatacacg gagcgcttgg acaggatgct gttcgggtcc
    22561 ttgagcgccg cggcgccgct ggcgggcctg ccggtgaggt cgacgccggc cggcggcgcg
    22621 gacggcgccg gctgcgctac ggcgcctggg gcgcggtctt ccacggccgc cggcggctgc
    22681 cggtccgggg tctgcgtcgc cgtgcaggcg gcgagggtgc aggcgatgaa ggcaaggctg
    22741 agcgttcgca tggctgatct ccttctcatt gcaacaaggg gccccaggcc ggctcgcgga
    22801 catcgcccgc gttgaccgtg aggcgctgct tgacgcgtcc gtcggcggac acggcggtga
    22861 gcacgccgcg cccgcccgcg atcgaggcga gcaggatctg gcggccgttc ggggcgaagc
    22921 tgggggactc gtcgaggtcg ctgtcggtga ggacctgcac ctggcgggtg cccaggtcga
    22981 gcagcgcaac ctggtacttg ctgccgttgc gcatgatgta ggcgagactc ctgccgtccg
    23041 gactgatgcg cggggagaca ttatagctcc cctcgaaggt cacgcgttgc gcgtcgccgc
    23101 cggccgccgc catgcggtag atctgcgggc tgccgccgcg gtccgaggtg aagtagatcc
    23161 actgcccgtc cggcgagtag cgcggctcgg tgtcgatgcc gccggaaaag gtcaggcggc
    23221 gcacgttgcc gccgtcggga ttgatcaggt agatctgcga agagccgtcg cgcgtcagcg
    23281 ccaccgcgag gcgcgtgccg tcgggcgacc aggcgggcgc ggagttcgag cccttgaaat
    23341 tggcgacgat gttctgcttg ggcacctcga cgtcctgcac gtagacgatc ggcttcttgt
    23401 tctggaacga aacgtaggcg atgcgcttgc cgttgggcga ccaggcgggc gagatgatcg
    23461 gctcgttgga caccagcatc gcctgctcgt tcgcgccgtc ggcgtcggcg atctgcagct
    23521 cgtagcgcga gccgcgcttc accacgtagg cgatgcgcgt ggagaacacg cccttctcgc
    23581 cggtgagctt ctcgtagacg tagtcggcga tgcggtgcgc cgtcgcgcgc agctgctcgc
    23641 gcgacatggt gtaggcgacg ccgccgatgg acgactgctt caccacgtcg tgcagccgga
    23701 agcgcgcctc gaagcgcccg tccgggcgcg ccgccaccga gccgagcacc agcgcatcgg
    23761 ccagccgcga cttccactcg gcgtagttga cgctcgaggc ctcggtcggc gccggcacca
    23821 gcgagggcac ctcgacgccg cggaacagtc cgctgcgttc caggtcggcg cgcacgatgg
    23881 cgctgatggc cgcgttcgcg ccgccggcca gcgcgccctc tcccacgaac ggcacgatgg
    23941 ccaccgggat gcgctgcgcc cccgcgccgg tgatctcgat cgagagctgg gcgtggccgg
    24001 agcacgcggc gaaggacagc gcggctgccg cgagcagatt tctcatgcgg tcgattatat
    24061 ttcagtccat cggccggaac gtcagcttca gctcgcgctc gaacaggccg ggactgtcgg
    24121 gcttaggcaa cggcgaggat ttgaggatcg cgcgcagcac cgcctcgtcg tagccggcgt
    24181 ggccgctgga aatcaccagc cgcgcctgca ggacttcgcc gtttggcagc agcgtcacga
    24241 ggaacagcgc ctccgggttt cccttgatgt ccagcggcag aaagatgttg ccgcggatct
    24301 tgttgcggat cctgtccacc caggccgcga ctcccttgaa gcgcgcattg gcggcatcgc
    24361 gcgtcagctg gtccttgatc gcctggcgct cgcgctccgc ggcgaactgg tgctgctcgc
    24421 gctgcgccgc ctcggcgagc agccgccgca gcgccgcgtc gtccgccttc gccgcgggct
    24481 tgggtttcgg cttggcctcg ggcttcggct cgggcttcgg cttgggcttg ggcgccttca
    24541 gcgcgatgtc cggcttctcg atgcgcggct ccggctttgg ctgcaccacc ggcgcgggct
    24601 tggggggctc gggctggacc gccgccggcg gcgggggcgg cggctgccac agctccacct
    24661 gcaccacctc gggcgcgcgg ctctgccagc gcacgccgaa ggtcagcacc accgccagca
    24721 gcaggtgcac cacgacggcg agcaccagcg cgggcgcgag gtcctcttcg cgccgcagct
    24781 cgaggaacgg cccggcgagc gccgcgctca ttgcgccggc ttcaccagca ggccgacgcg
    24841 cttcacctgc agcttgtgca gctcgtccat cacctgcagc accgtctcgt agcgcacgtc
    24901 cttgtcgccg gcgatcagca ccggctgctc cgggttcttc gccaggcgct ccttgatcac
    24961 gcgcgccagg tccgcgcgcg gcacgcgccg ctcctcgccc gggacgccct tggcgtcgcg
    25021 gctgcgcagc aggatcgagc ggtcggcgcg gatcaccacc tcgagcggcg acaccggcgc
    25081 gttggaggcc ttggacaccg agggcaggtc gatcacgccg ggggtcacca gcggcgcggt
    25141 caccatgaag atgacgagca gcaccagcat cacgtccacg tagggcacga tgttgatctc
    25201 gtgcatcggg gcgcgcttgc gatgcagcgc cggcatggtt acttcaccgc ctgccgctgc
    25261 aggatgttgg agaactcctc catgaagctc tcgaagcgga tggcgaggcg gtcgatctgg
    25321 tgcgagtagc ggttgtaggc caccaccgcg gggatagcgg cgaacaggcc gatggcggtg
    25381 gccaccagcg cctcggcgat gccgggggcg acctgcgcca gcgtcgcctg ctgcacgttc
    25441 gccaggctgc ggaaggcgtg catgatgccc cacaccgtgc cgaacaggcc gacgtagggg
    25501 ctcaccgagc cggtggaagc gaggaacgcg aggtggcgct cgaggtggtc cgtctcgcgc
    25561 tggtaggtcg cgcgcatggc gcgccgcgcg ccgtccacca tcacgctggg gtcggtggtg
    25621 ctgcgctgat tcctcagctt ggcgaactcg cggaagcccg cctcgaagat gcgctccagg
    25681 ctgccgatgg tgtggcggtt gttcaccgcg ccctggtaga tcaggttcag gtccttgccg
    25741 ccccagaact cgcgctcgaa ctgctcggtc tgcccgcgcg cccggcggat ggcgaaccac
    25801 ttgctgaaga tgaacatcca ggacaggaac gacaccgcga gcagcagcag catcaccgcc
    25861 ttcaccacca ggctcgcgcc gaggatcagg gtccagatgt cgaggtcggc ggtgacattc
    25921 atggcttcag cccagttgcg cggcaaaggt ggccgggatc gcggcgggcc ggaaatcgct
    25981 ttgccgcacg cacgccgctt ggaccagcgc ctcggccatc ggttcagtgc cgcgcagcgc
    26041 gcgctgcttg agcatcaggt agctgcgctt gcgttcagtc agttccacgg tgatctccag
    26101 ttggtcgtcg agccgcgcgc cgcgcaggaa gtcgatttcc gcgcgccgca ccacgaattg
    26161 cagccccgcc tcgcgcgcca ggccctggtg gtcgatgccg agggtgcgca gccactcggt
    26221 gcgcgcgcgc tcgaagtagc gcaggtaatt cgcgtagtac accacgccgc cgaagtcggt
    26281 gtcctcgtaa tagacccgta ccggccagga aaagactctg gcgtcccgtc gctgcaccgc
    26341 gcgattgtag tccgcgccgc tcagccgcgc gcgtcggggc cgagcaagtc gtgcgccccc
    26401 gacggcgcga cgaggccgaa gtggcggtag gcggcgaggg tcgccacgcg cccgcgcgcc
    26461 gtgcgctgca ggtagccctg ctggatcagg tagggctcga gcacgtcttc gatggtgtcg
    26521 gactcctcgc cgatggcgtg cgccaggttc tccacgccca ccgggccgcc gccgaaccgc
    26581 tcgatgacgg cgagcaggag cttgcggtcc atcacgtcca ggccgagcgc atccacgtcc
    26641 agcatcttga gcgcagcctc ggccaccgcc ctggtcaccg cgccctcggc cttgacctgc
    26701 gcgtaatcgc gcacccggcg cagcagccgg ttggcgacgc gcggcgtgcc gcgcgcgcgg
    26761 cgcgcgatct cggcgccacc ctcggtcgcc agcgtcaccg ccagcagccc cgccgagcgg
    26821 cgcacgatcg ccgcgagctc gggctcgctg tagaactcca gccgggcgac gatgccgaag
    26881 cggtcgcgca gcggattggt cagcatgccg gcgcgcgtgg tggcgccgac cagcgtgaac
    26941 ggcggcaggt cgagcttgat ggagcgcgcc gccggtccct cgccgatcat gatgtcgatc
    27001 tggtagtcct cgagcgccgg gtacaggatc tcctcgacca ccggcgagag gcggtggatc
    27061 tcgtcgatga acaggacgtc gcgcggctca aggttcgtca acagcgccgc caggtcgccc
    27121 gggcgctcca gcaccggccc ggaggtctgg cgcagattca cccccatctc gcgggcgatg
    27181 atgtgcgcca gggtggtctt gcccagtccc ggcgggccga acagcagcac gtggtccagc
    27241 gcctcgctcc tgccgcgcgc ggcctcgatg aagatcccca gctggtcgcg caccttggcc
    27301 tggccgacgt agtccgccag ccgcacgggc cgcagcgagc gctcgaactg ctcctcctgc
    27361 gccgaggccg gggcggcggc gatcaggcga tcggtctcga cggacatggc ggaattctgc
    27421 cagacccggg acgtttgccc ttgaagatca cccgggacag gcatacgatt tcttccgtgg
    27481 caaccatccg gaccttcgtc gcagcgctgt cggctgcctt ctggctgccc ggcgccctcg
    27541 cccaggcgcc ggcggacaac ggctggtacc tcggctcggg cctgctcccg gtcaagcccc
    27601 ggctgcagct cgcggggccg gcgatccccg gcgagccgcg cgaggccgcc gagcccggct
    27661 accagcagtt cgggggcttc cggtttacgc gcaactggag cctcgacatc ggctatgccg
    27721 ggggcaaccg cccggctgcg ggtgacggct cgatccacgc caacgcctgg accttctccg
    27781 gcacgggcct gctgccgatc acccgctcgt tctcgctgca gggccggctg ggcgtcgcga
    27841 tgcccaccgc cgagttctcg ctgacatcgg cggccagcac cctgtcctcg ctcgccgggg
    27901 acagcgcgcg caaccgcatg aacatgctgt ggggagtcgg gggccagtac gaattcagcc
    27961 ccacggtcgg cctgcggatg gactacaaca gccgctacgc cgacgacacg ggctacaacc
    28021 gcgcgcgcac cgacctgtgg tcgatcaacg ccgtggtccg cttctagggc agttcaggac
    28081 ttggccagcg ccttgagcgc ggcgcggatg ccctcggcga ccggcaatcc cggcgccaat
    28141 ccctgcagag ccgcggcggc ctccttctcg ctgtagccca gcccgagcag ggcattgagc
    28201 acgtccgcgg cgccgccgcc gaccgccggc ctcgcgtcgg cgagcttgcc cttgagctcc
    28261 agcagcagcc gctcggcggt cttcttgccg atgccgggaa ccttggtgag gcgcgccggc
    28321 tcctgcagcg ccaccgcctg ggcgagctcc ggcaccgaca tgcccgacag aaccgagagc
    28381 gcggtgcgcg cgcccacccc cgagatcttg agcagctggc ggaacgcggc gcgctcctcc
    28441 agcgtgccga agccgtagag cgcctgcacg tcctcgcgca ccaccaggtg ggtgagcagc
    28501 accacgggct ggccggtcgc cggcaggttg tagaaggtgc tcatcggcac gtcgagctcg
    28561 taggcgacgc cgttgcagtc caccatcacc tgcggcggat gcttggcgac gagcgtgccg
    28621 ctgagccgcc cgatcacgga cggcgctcct ctcgcctgcc gatcatgcca gccgcccgcg
    28681 cttcatgcgc agcccgtgca tcggcagggc gcccaggcgt cccccgtgcg catggcagat
    28741 ggcgcaggcg agcgcatcgg ccgcgtccgc gccgggagac ccgggcaagg cgagcagccg
    28801 cttcaccatg tgctggacct gctccttctt ggcgtggccg ttgccgacca ccgcctgctt
    28861 gacctgcaga gcagtgtact cggcgaccgg cagcccggcg agcaccgccg cgcagatggc
    28921 ggtgccgcgc gcctggccga gggcgagggt cgactgcggg ttgacgttga cgaacacttt
    28981 ctcgacgacc acctgcgccg ggcgatgcgt ggcgatcact tcggccaggc cctcgagcag
    29041 gaccttcagc cgctcggcca actcgccgcg gcccgacttc acgcagccgc tggccacata
    29101 gtcgaggcgc cggccgctgt gctcgatgac accgaaaccc gtgacctgca ggccgggatc
    29161 gatgcccagg attctcacgc gtcctcgaac gccgcggtgg tatacacgtc ctgcacgtcg
    29221 tccagaacct ccagcgcatc gagcaggctg cgcatgcgct gcgcatcgcc gccggcgagc
    29281 gcgctgtcgt tgaccggctt catggtcacc tcggccagct cgggcttcag tcccgccttc
    29341 tccagagccg ccttgacctg gtggaagtcg tgcggcgcgc tgagcacctc gatcgagccg
    29401 tcgtcgttcg ccaccacgtc ctcggcgccc gcctcgagcg ccgcctccat caccttctct
    29461 tcgctcgcgc caggggcaaa catgaacagg ccgcaatgct tgaacatgaa cgccaccgag
    29521 ccgtccgagc cgaggttgcc gccgttcttg gtgaaagcgt ggcgcacatc ggccacggtg
    29581 cgcgtgcggt tgtcggtcag gcaatcgacg atcagcgcgg cgccgccggg accgtagccc
    29641 tcgtagcgca cttcctcgta ggacacgccc tccagctggc cggtgccgcg cttgatggcg
    29701 ttctgcacgc tgtccttggc catgttggcc tcgcggccct tgtccatcgc caggcgcagg
    29761 cgcggattca tgttcgggtc gccgccgccc atcttcgccg cgacggtgat ttccttgatc
    29821 agccgggtga agaccttgcc cttcttcgcg tcggccgcgg ccttgcggtg cttgatgttc
    29881 gcccatttgc tgtgcccggc catgccataa ttttacgtca tggccaccgc actcctcgtc
    29941 gccagaagcg gcaagaccga cctcggcctg ctgcccgcgc tcgccaaccg ccatggcctg
    30001 atcaccggcg ccaccggcac cggcaagacc gtgacgctgc agacgctcgc cgaacagttg
    30061 tcgcgcatcg gggtcccggt gttcatggcc gatgtgaagg gcgatctgtc gggcatggcg
    30121 aaagccggcg gcgcgaacgc caaggtagcc gagcgcgcca agctgctcaa gctcgaattg
    30181 acctacgcgg cctgccccct ggtgttctgg gacgtgttcg gcgagagcgg ccacccggtg
    30241 cgcgccaccg tctcggacat ggggccgctc ctgctcgcgc gcatgctggg cctcaacgac
    30301 acccaggaag gcgtgctgaa cctggtgttc aagatcgccg acgacgcggg cctgctgctg
    30361 ctcgacacca aggacctgcg cgccatcctg cagttcgtcg gcgagaacgc cgcgaaattc
    30421 accaccgaat acggcaacgt ttcgacggcc tcgatcggcg ccatccaacg cgggctgctg
    30481 acgctggaga cgcagggcgc cgaccgcttc ttcggcgagc cgatgctcaa catcgccgac
    30541 ctgatgcagg tggaaggcgg caagggagtg gtgaacatcc tcgccgccga ccggctgatg
    30601 aattcgccca agctctacgg cacgttcctg ctgtggatgc tggcggagct gttcgagcag
    30661 ctgcccgaag tcggcgacgt ggacaagccg aagctcgtgt tcttcttcga cgaggcgcac
    30721 ctgctgttcg acgacgcgcc cagggcgctg ctcgacaagg tcgagcagat ggtgcgcctg
    30781 atccgctcca agggcgtggg cgtgtacttc gtgacgcaaa acccgctcga catccccgag
    30841 tcggtgctcg ggcagctcgg caaccgggtg cagcacgcgc tgcgcgcctt cacgcccaag
    30901 gaccagaagg cggtgaagtc cgccgccgag accatgcgcc ccaacccgaa gctcaggatc
    30961 gagcaggcga tcaccgagct cggcgtgggc gaagcgctgg tgagcctgct cgacgaaaaa
    31021 ggccgccccg gggtcaccga gcgcgcgttc gtcgtgcctc cgggcagcca actcgggccg
    31081 ctcaccgacg ccgagcgcaa ggccgccatc gcgtcctcgc cgctcgcggg gcactacggg
    31141 aaagcggtgg accgcgaatc ggcctacgag aaactcaagg cgcgcgcaga ccaggcggcg
    31201 caggaggccg cagcgcagac aacgcaggcg cagccgaagg aatcgagcgt cgcctcggac
    31261 atcctgttcg gcaagacggg cccgcgcggc ggccggcaat cgcagggact gctcgaagcc
    31321 atggcgaaga gcgcggcgcg cactgtcggc tcgcaggtcg ggcgcgagct gatccgcggg
    31381 gtcctgggct ccttgctcgg caaacgccgt tgagtcccgc ggcgtaccgc ggctgggggc
    31441 tgctgttcgc gctcgccggc acggtggcct tcgccttccg cccggtgctg atcaagctgg
    31501 gctacgaggc ggcgccggtg agcgcgacga cgctgctgtt cctgcgcatg gtgctgtcgc
    31561 tgccgttttt cgccgccgtg gcctggtggt tgcgccgcga accgggactc gcgcgccgcg
    31621 actggctggg catcgtcggc ctgggcttcc tcggctacta cctcgccagc ctgctcgact
    31681 tcctcgggct gcagtacgtg cccgccggcc acggacggct gatcatgttc ctctacccga
    31741 cgctggtggt gatcctgtcc gcgctgttcc tcggcaagcg cgccaccgcg cgcgagctcg
    31801 ccgcgctcgc cgtgacctac accggcatcg cgctggtgct gtcgcaccag ctcggcgcgc
    31861 cggccgagaa ccgcctgttc ttcctcggcg cggcgctcgt cttcggctcg gccatgtgct
    31921 acgccgtgta cctggtcacc ggcagccagc tcatcaagcg cgtcggctcg atgcgcttca
    31981 ccgcttacac catgatggtg tccaccgtcc cggcggtgat ccagttcggg gtgctggagg
    32041 gcccggcgtc gctctcgctg ccgggcatcg tctggatcta cgccatcgtc ctggcgacgg
    32101 tgtgcacggt actgccggtg ttcctggtcg ccgaatcgct caagcgcatc ggcgccagcc
    32161 agttcgcgct catcggcggc ctggggccgg tcaccaccgt gctggccgat ttcgcgctgc
    32221 tcgagggttc gctgtccgtg ctgcaggtgc tcggcggcgc gctggtcatc ggcggcgtct
    32281 tgctcgtcac actgaagaaa ggctgaacct catggacctc ggaatcaagg gaaggaacgc
    32341 attggtctgc gccgcctcga agggcctcgg caagggctgc gcgatggcgc tggcgcgcga
    32401 aggcgtgagc ctcgtgatca ccgcgcgcgg caaggaggcc ctggaggcga cggcggagga
    32461 gatccgccgg gccaccgggg cgaaggtcgt cgccgtcgcc ggcgacatca ccacggcgga
    32521 gggccgcgcg caggcgctcg ccgcctgtcc gagccccgac atcctggtga acaacgccgg
    32581 cggcccgccg ccgggggatt tccgcaactg gacgcgcgag gactggatca aggcggtgga
    32641 cgccaacatg ctgacgccga tcgagctcat caaggcgacc atcgacgcca tgatcgcgaa
    32701 gaagttcggc cgcatcgtca acatcacctc cggcgccgtg aagatgccga ttccggagct
    32761 gggactgtcg aacggcgcgc gcaccggcct caccggcttc gtcgccgggc tcgcgcgcac
    32821 cacggtgcag cacaacgtca cggtgaacaa cctcctgccc gggcccttcg acaccgatcg
    32881 cctgcgctcg aacatcgagt tcaacgcgaa gaagctcggc aaaaccccgg ccgagttcgc
    32941 gcaggcgcgc cgcgaggcca acccggcaaa gcgcttcggc acggcggagg agttcggcga
    33001 cgcctgtgca ttcctttgca gcgcgcaggc ggggtacatt acgggccaga acctgctgct
    33061 ggacggcggg gcctatccgg ggactttctg atgaaaacca tcgcggcaat cctgacggcg
    33121 ctgtttctcg ccggcgcagc ctg
//
LOCUS       DASRIE010000151         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_936088, whole genome shotgun sequence.
ACCESSION   DASRIE010000151 DASRIE010000000
VERSION     DASRIE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936088"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFB53_06450"
     CDS             <1..1300
                     /locus_tag="VFB53_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86407.1"
                     /translation="GAVAPSHEGMLHGLRQLLAMRVAAPLAQGATLLVATAGHGIPVP
                     VAAAAALLAFDAAVAAATWRRVRRAPRIGVPEIVAQSLLDVLTLAAMLYLTGGANNPF
                     AMLLLLPVAIAASAAPAHGAALTAAASTAAVLVLVERHLPLAHPAGDAALQALLRAGW
                     VASFALTAALLAVVVSGTHAAMRRREQLLATAREAQMRNEYTSSIGELAARCAHELSS
                     PLSTMAVVVGELLREQRGNAPLLRDLRLLHGQIQASKQIVSNLANAAGHRRAESAGGA
                     KLDQFLHAIVDRARALHPGATIAESLDDATPPPCILAEETLRQAVTSLIDHAVQCSPH
                     QVQVSADWSGTDLRVVVRDRGPGFAPELLDRLEQPLDAVHAGEAGSGVLLSAVTLQRM
                     GGRLALVNEPIRGARAELTLPLRTISIEKTRGRVDGQPVA"
     gene            1281..1829
                     /locus_tag="VFB53_06455"
     CDS             1281..1829
                     /locus_tag="VFB53_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN86408.1"
                     /translation="MDNPSHDRPRLLLADDDRTFCQVLARAFDARGFAVDVVHDGRTA
                     VQLINRTVPDYAVIDLRLPDVSGLKLVEHLKHLAEHVPVVVLTGYGSIATAIEAIKLG
                     ATHYLTKPSDADQIVAAFAHRKANPDVPAAERPLSVDRVEWEHIQRVLAGFDGNVSAT
                     ARALDMHRRSLQRKLNKHPPRE"
     gene            1836..2312
                     /gene="gspG"
                     /locus_tag="VFB53_06460"
     CDS             1836..2312
                     /gene="gspG"
                     /locus_tag="VFB53_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HZN86409.1"
                     /translation="MRPAFTTHTPSGVPVENAPASLRRAARGFTLLELLVVMVIIGLL
                     ASYVGPRYFAQIGKSEAKAARAQIDAFEKALEQYRIDTGHFPSAEQGLTALNVKPNDE
                     PRWSGPYLKKAAPPDPWGHPYQYKAPGEHGGEYDLFSLGKDRRAGGTGEDADIGNW"
     gene            2322..3509
                     /locus_tag="VFB53_06465"
     CDS             2322..3509
                     /locus_tag="VFB53_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN86410.1"
                     /translation="MKFNVKALRGAGDVVALTLQAADIDEARRHADRQGLRVLSIKAS
                     NPLAGWLKPRTTFDLELFSQELVSLLDAGLSLVEAIELLAEKEQARGAVTALNELNAD
                     LHRGLKLSDALARQAAVFPPLFVATVRAAERTGDIQEAIGRFLQHHVQIGRIRRQVVA
                     ASVYPVLLMGVGVLVSGFMLLYVVPKFSQIYEEIGTNLPFLTRVLVGWGQVLEAHATL
                     ALGTAAALVGGTVFVFTRPGVRQWMGPQLWKVPALGNRLRIYELARFYRTLGMLLDSG
                     IPLPTAIDMAGGLLSPMLRGALQRAAAQVREGQAVSRAFQQHGLTTQVAFRLLAVGER
                     TGRMPHMVERIALFYEDELARWIGWFTKLFEPVLMVMLGALVGLIVILLYLPIFELAE
                     NVK"
     gene            3635..>5295
                     /locus_tag="VFB53_06470"
     CDS             3635..>5295
                     /locus_tag="VFB53_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN86411.1"
                     /translation="MDLAVAPQTFRAEPFPADVIAAARAEARAAGTRVVDALEAGAGL
                     QPAEFVRRLAATVRFPAMTMDDLHALAAAFDAMPFAEAAAHECLALRDAAGALVVVFG
                     DPFDPGVRAWAEERIAADFTFRLAHPADIRAYLARHEESLRAMDAVLGASMGGEDAHA
                     ENAETLSLTSISEDDSPVVKLVSSTIYDALKSQASDIHVETTPAGLTIRYRIDGVLVA
                     VGNVGSPEIAEQVLSRIKVMAELDIAERRVPQDGRFKVSVKGRTIDLRVSVMPSIFGE
                     DAVLRILDKQSLTAEMLGLLLTNLGFDADSIARMRRLAREPYGMLLVTGPTGSGKTTT
                     LYATLSEINSGQDKIITIEDPVEYQLPGVLQIPVNEKKGLTFSRGLRSILRHDPDRIL
                     VGEIRDSETAEIAVQAALTGHLVFTTVHANNVFDVIGRFRHMGVDPFTFVAAMNGIVA
                     QRLVRKVCTECAVEDHPDDATLAASGLTRERVRGFGFRMGRGCGRCRGTGYRGRRAIA
                     EILMMNDEIREMILRNEPVRLLREAAARNGTHFLREVALDCVRRG"
BASE COUNT          732 a         1973 c         1862 g          728 t
ORIGIN      
        1 cggcgcggtc gcgccgtcgc acgaggggat gctgcacggc ctgcggcagc tgctcgcgat
       61 gcgcgtcgcc gcgccgctcg cgcagggcgc gacgctgctc gtcgcgacgg cggggcacgg
      121 tatccccgtg ccggtcgcgg ccgccgccgc gctgctcgcg ttcgacgcgg ccgtggccgc
      181 ggccacgtgg cgacgcgtgc gtcgcgcgcc gcgcatcggc gtgccggaga tcgtcgcgca
      241 gtcgctgctc gacgtgctga cgctggccgc gatgctctac ctcaccggcg gcgcgaacaa
      301 tccgttcgcc atgctgctgc tgctgcccgt ggcgatcgcc gcctcggccg caccggcgca
      361 cggcgcggcg ctcaccgcgg cggcgtccac ggcggcggtg ctcgtcctcg tcgagcgcca
      421 cctgccgctc gcgcacccgg ccggagacgc cgcgctgcag gcgctgttgc gcgccggatg
      481 ggtcgcgagc ttcgcgctca ccgcggcgct gctcgccgtc gtggtgtccg gcactcacgc
      541 ggccatgcgc cggcgcgaac agctcctcgc gaccgcgcgc gaggcgcaga tgcgcaacga
      601 gtacacctcc tcgatcggcg agctggcggc gcgctgcgcc cacgagctga gctcgccgct
      661 gtccaccatg gcggtggtgg tcggcgagct gctgcgcgag cagcgcggca acgcgccgct
      721 gctgcgcgac ctgcgcctcc tgcacggcca gatccaggcg agcaagcaga tcgtctcgaa
      781 cctcgcgaac gcggccggcc accggcgcgc cgagtcggcc ggcggcgcca agctcgacca
      841 gttcctgcac gcgatcgtcg accgcgcccg cgcgctgcac cccggcgcga ccatcgccga
      901 gtcgctcgac gacgccacgc cgccgccctg catcctcgcc gaggagacgc tgcgccaggc
      961 ggtcaccagt ctcatcgacc atgccgtcca gtgctcgccg catcaggtgc aggtgagcgc
     1021 cgactggtcc ggcacggacc tgcgcgtcgt cgtgcgcgac cgcgggccgg gcttcgcgcc
     1081 cgagctgctc gaccgcctcg agcagccgct ggacgcggtg cacgcgggcg aggccggcag
     1141 cggcgtgctg ctgtccgccg tcacgctgca gcgcatgggc ggccggctgg cgctggtcaa
     1201 cgagccgatc cgcggcgcgc gcgccgagct gacgctgccg ctgcgcacga tatcgatcga
     1261 gaagacaaga ggaagagtcg atggacaacc cgtcgcatga ccgcccccgg ctgctgctgg
     1321 ccgacgacga ccgcaccttc tgccaggtgc tcgcgcgggc gttcgacgcc cgcggcttcg
     1381 ccgtcgacgt ggtgcacgac ggccgcaccg cggtgcagct catcaaccgc accgtgcccg
     1441 actacgccgt gatcgacctg cgcctgcccg acgtgtccgg gctcaagctg gtcgagcatc
     1501 tcaagcacct ggccgagcac gtgccggtcg tcgtgctgac cggctacggc agcatcgcca
     1561 ccgcgatcga ggcgatcaag ctcggcgcga cgcactacct caccaagccg agcgatgccg
     1621 accagatcgt cgccgcgttc gcgcaccgca aggccaaccc cgacgtgccc gccgccgagc
     1681 gcccgctgtc ggtcgaccgc gtcgagtggg agcacatcca gcgcgtgctc gcgggcttcg
     1741 acggcaacgt gtcggccacc gcgcgtgcgc tcgacatgca ccgccgctcg ctgcagcgca
     1801 agctgaacaa gcatcccccg cgggagtagc gcgccatgcg tcccgccttc accacccaca
     1861 cgccgtccgg agtccccgtg gaaaacgccc ctgcctccct gcgccgcgct gcccgcggct
     1921 tcacgctgct cgagctgctc gtcgtgatgg tcatcatcgg cctgctcgcg agctacgtcg
     1981 gcccgcgcta cttcgcgcag atcggcaagt ccgaggcgaa ggcggcgcgc gcgcagatcg
     2041 acgccttcga gaaggcgctc gagcagtacc gcatcgacac cggccacttc cccagcgccg
     2101 agcagggcct gaccgcgctc aacgtcaagc cgaacgacga gccgcgctgg agcggcccgt
     2161 acctgaagaa ggcggcgccg cccgacccgt ggggccaccc gtaccagtac aaggcgcccg
     2221 gggagcacgg cggcgagtac gacctgttct ccttgggcaa ggaccggcgc gccggcggca
     2281 ccggcgagga cgccgacatc ggcaactggt aggaccgcgc ggtgaagttc aacgtcaagg
     2341 ccttgcgcgg cgccggcgac gtcgtcgcgc tgacgctgca ggccgcggac atcgacgagg
     2401 cgcgtcgcca tgcggaccgg cagggcctgc gcgtgctgtc gatcaaggcg agcaacccgc
     2461 tcgccggctg gctgaagccg cgcacgacgt tcgacctcga gctgttcagc caggagctgg
     2521 tgtcgctgct cgacgccggc ctgtcgctgg tggaggcgat cgagctgctc gccgagaagg
     2581 agcaggcgcg cggggccgtg accgcgctga acgagctgaa cgccgacctg catcgcggcc
     2641 tgaagctgtc cgacgcgctg gcgcgccagg ccgcggtgtt cccgccgctg ttcgtcgcca
     2701 ccgtgcgcgc ggccgagcgc accggcgaca tccaggaggc gatcgggcgc ttcctgcagc
     2761 accacgtgca gatcggccgc atccgccgcc aggtggtggc ggcctcggtg taccccgtgc
     2821 tgctgatggg cgtgggcgtg ctggtgtcgg gcttcatgct gctctacgtg gtgcccaagt
     2881 tcagccagat ctacgaggag atcggcacca acctgccgtt cctcacgcgc gtgctggtgg
     2941 gctgggggca ggtgctcgag gcgcacgcga cactggcgct cggcacggcc gccgcgctcg
     3001 tgggcggcac ggtgttcgtg ttcacccggc ccggcgtgcg ccagtggatg gggccgcagc
     3061 tgtggaaggt cccggcgctc ggcaaccggc tgcgcatcta cgagctggcg cgcttctacc
     3121 gcacgctcgg catgctgctc gacagcggga tcccgctgcc gacggccatc gacatggcgg
     3181 gcggcctgct gtcgccgatg ctgcgcggcg cgctgcagcg cgcggcggcg caggtgcgcg
     3241 aggggcaggc ggtgtcgcgc gcgttccagc agcacgggct cacgacgcag gtggcgttcc
     3301 gcctgctcgc ggtgggcgag cgcaccggcc gcatgccgca catggtggag cgcatcgccc
     3361 tgttctacga ggacgagctg gcgcgctgga tcggctggtt caccaagctg ttcgagccgg
     3421 tgctgatggt gatgctcggc gcgctcgtcg gcctgatcgt gatcctgctg tacctgccca
     3481 tcttcgagct cgccgagaac gtcaagtagc cacgtcaaag gaccgtcgat gcgccgcctc
     3541 acgtccttgc cctgcgcgct tttgccgcag ccgcgccgcc cgcaagcgcg cagggcgacg
     3601 ggtagcatgc ctgcactctc gcggaagtaa tcgtatggat ctcgccgtcg cgccgcaaac
     3661 cttccgggcg gagccgtttc ccgccgacgt gatcgcagcg gcccgcgcgg aggcccgggc
     3721 ggcgggcacg cgcgtcgtcg acgcgctgga agcgggtgcg ggcctgcagc ccgccgagtt
     3781 cgtgcgccgc ctcgcggcca cggtgcgctt ccccgccatg acgatggacg acctgcacgc
     3841 gctggccgcg gcgttcgacg cgatgccctt cgccgaggcc gccgcgcacg aatgcctcgc
     3901 gctgcgcgac gcggccggcg cgctcgtcgt cgtgttcggc gatccgttcg accccggcgt
     3961 gcgcgcctgg gccgaggagc gcatcgccgc cgacttcacc ttccgcctgg cgcacccggc
     4021 cgacatccgc gcgtacctcg cgcgccacga ggaatcgctg cgcgcgatgg acgcggtgct
     4081 cggcgcctcg atgggcggcg aggacgcgca cgccgagaac gccgagacgc tgtcgctcac
     4141 ctcgatcagc gaggacgaca gcccggtggt caagctggtc agctccacca tctacgacgc
     4201 gctcaagtcg caggcgagcg acatccacgt cgagaccacg ccggccgggc tcaccatccg
     4261 ctaccgcatc gacggcgtgc tggtggcggt gggcaacgtc ggcagcccgg agatcgccga
     4321 gcaggtgctc tcgcgcatca aggtgatggc cgagctcgac atcgccgagc gccgcgtgcc
     4381 gcaggacggt cgcttcaagg tgtcggtcaa gggccgcacc atcgacctgc gcgtgtcggt
     4441 gatgccgagc atcttcggcg aggacgcggt gctgcgcatc ctcgacaagc agtcgctcac
     4501 ggccgagatg ctcgggctgc tgctcaccaa cctcggcttc gacgccgaca gcatcgcgcg
     4561 catgcgccgc ctcgcgcgcg agccgtacgg catgctgctc gtgaccggcc ccaccggcag
     4621 cggcaagacg accacgctct atgccacgct gagcgagatc aacagcggcc aggacaagat
     4681 catcaccatc gaggacccgg tggagtacca gctgcccggc gtgctgcaga tcccggtgaa
     4741 cgagaagaag gggctcacgt tctcgcgcgg cctgcgctcg atcctgcgcc acgacccgga
     4801 ccgcatcctc gtcggcgaga tccgcgactc ggaaacggcg gagatcgcgg tgcaggccgc
     4861 gctcaccggc cacctcgtgt tcaccaccgt gcacgccaac aacgtgttcg acgtgatcgg
     4921 ccgcttccgc cacatgggcg tggacccctt caccttcgtc gccgcgatga acggcatcgt
     4981 cgcgcagcgc ctggtgcgca aggtgtgcac cgaatgcgcg gtcgaggacc accccgacga
     5041 cgcgacgctc gccgcctccg gcctcacgcg cgagcgcgtg cgcggcttcg gcttccgcat
     5101 ggggcgcggc tgcggccgct gccgcggcac cggctaccgc ggccggcgcg cgatcgccga
     5161 gatcctcatg atgaacgacg agatccgcga gatgatcctg cgcaacgagc cggtgcggct
     5221 gctgcgcgag gcggcggcgc gcaacggcac ccacttcctg cgcgaggtgg cgctcgactg
     5281 cgtgcggcgc ggcga
//
LOCUS       DASRIE010000152         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1211743, whole genome shotgun sequence.
ACCESSION   DASRIE010000152 DASRIE010000000
VERSION     DASRIE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211743"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="ribD"
                     /locus_tag="VFB53_06475"
     CDS             complement(<1..428)
                     /gene="ribD"
                     /locus_tag="VFB53_06475"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZN86412.1"
                     /translation="MSAFTAFDHAMMRRALALAEKGLYTATPNPRVGCVLVNGGQVIG
                     EGFHEKTGGPHAEAHALVQASAAARGATAYVTLEPCNHHGKTPPCAQALIKAGVARVV
                     AAMRDPNPGSKQGGAALAAAGIPFEPGLLEEEARELNIGFV"
     gene            complement(425..889)
                     /gene="nrdR"
                     /locus_tag="VFB53_06480"
     CDS             complement(425..889)
                     /gene="nrdR"
                     /locus_tag="VFB53_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZN86413.1"
                     /translation="MRCPFCGNEDTQVLDTRSSTEASAIRRRRKCPSCDKRFTTYENV
                     DLKMPRLVKKDGSRTEFARDKLTGSMMLALRKRPVATEALEAAVQRIEDRLRSLGERE
                     VPTSRVGDLVMRELAKLDKIAYIRFASVYRSFETPEEFREAVQEVKTKKRRK"
     gene            complement(901..2142)
                     /gene="glyA"
                     /locus_tag="VFB53_06485"
     CDS             complement(901..2142)
                     /gene="glyA"
                     /locus_tag="VFB53_06485"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZN86414.1"
                     /translation="MSASDLLARTDPELWDAIAQENRRQEDHVELIASENYVSPAVLA
                     AQGSRLTNKYAEGYPGKRYYGGCEYVDLAETLAIARARKLFGADWANVQPHSGSQANQ
                     AVYVTVLKPGDKVLGMSLAHGGHLTHGSSVNLSGKLYQFISYGLDAKEEIDYDAAEQL
                     ALEHRPRLIVAGASAYSLRIDWRRFRDIADRCGALLMADIAHYAGMVAVGLYPSPIGV
                     AHYVTSTTHKTLRGPRGGLVLGMGDTGKGIDSAIFPGLQGGPLMHVIAAKAVAFGEAL
                     RPEFKSYQQQTLDNARAMARVLTARGVRIVSGRTESHMMLLDLRGKKITGKDAEAALG
                     RAHITVNKNAIPNDPEKPTVTSGVRIGSPAMTTRGFRVAEAEILAGLVADVLDRPADD
                     GHLRRVAGEVQALCARFPVYA"
     gene            complement(2180..3079)
                     /locus_tag="VFB53_06490"
     CDS             complement(2180..3079)
                     /locus_tag="VFB53_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN86415.1"
                     /translation="METAQRAVLFADVVDSTRIYESLGDTRALALINSLFGLLDKAAS
                     RHGGAVVKKLGDGMVCLYPDAGAAFQSACDMQMEATSPAHAAERLGIKVGFTFGPVVL
                     SEGDVFGDTVNVCARLVALANAEQVLTTQLTVDALPPGLRGRCRQMFPIRVRGRHEEI
                     IACSVLWRSDPDVTETNLARAKLTRSRETVLKITSQGEVRVVRSSDSGMRIGRDKVND
                     LVVDSHFASRVHARLFAREGHFVLQDLSSNGTYLLSDGNSAEILLRREEAVLSDRGWI
                     GLGNTAARHGDHTLRYRVEPEGD"
     gene            complement(3114..3692)
                     /locus_tag="VFB53_06495"
     CDS             complement(3114..3692)
                     /locus_tag="VFB53_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN86416.1"
                     /translation="MKFAGLLLAAGAATRFGSDKLQHALPHGVAIAVQAARHLRAELP
                     RVLAVVRPGADATARALAGEGCEVVVCERAGEGMGASLACAARAAGAADGYLVALADM
                     PFVRPSTIAAVRDALAQGAILAAPYWRARRGHPVGFAGSLRAELEALAGDAGAKSVLA
                     AHAAQLVKVPVGDPGALRDIDRPEDLAPPLAV"
     gene            complement(3689..>3965)
                     /locus_tag="VFB53_06500"
     CDS             complement(3689..>3965)
                     /locus_tag="VFB53_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN86417.1"
                     /translation="GLKSAAFYVGAIGSKKNNDARRKRLLEFDLAEAEIARLRGPVGL
                     YIGSKTPPEIAVAILAELTAVRHGVSEPSWSAKKDARFDPSACEIKT"
BASE COUNT          530 a         1422 c         1402 g          611 t
ORIGIN      
        1 acgaagccga tgttgagctc gcgcgcctcc tcctccagca gccccggctc gaacgggatc
       61 cccgccgcgg cgagcgccgc gcccccctgt ttcgagcccg gattggggtc gcgcatcgcg
      121 gcgaccacgc gcgccacccc ggccttgatc agcgcctgcg cgcagggcgg cgtcttgccg
      181 tggtggttgc agggctcgag cgtcacgtag gcggtggcgc cgcgcgccgc ggcgctcgcc
      241 tgcaccagcg cgtgcgcctc ggcatgcgga ccgcccgtct tttcgtggaa gccttcgccg
      301 atcacctgcc cgccattcac cagcacgcaa cccacgcggg gattaggggt cgcggtatac
      361 agccccttct ccgcgagagc cagcgcgcgg cgcatcatcg cgtggtcgaa cgcggtgaag
      421 gcgctcactt ccggcgtttc ttcgtcttca cttcctgcac cgcctcgcgg aactcctcgg
      481 gcgtctcgaa gctgcggtac acggacgcga agcggatgta ggcgatcttg tcgagcttgg
      541 ccagctcgcg catcaccagg tcgccgacgc ggctggtggg cacttcgcgc tcgccgagcg
      601 agcgcaggcg gtcctcgatc ctctgcaccg cggcctccag cgcctcggtg gccaccgggc
      661 gcttcctgag cgcgagcatc atgctgccgg tgagcttgtc gcgcgcgaac tcggtgcggc
      721 tgccgtcctt cttcaccagg cgcggcatct tcaggtccac gttctcgtag gtggtgaagc
      781 gcttgtcgca ggacgggcac ttgcgccggc ggcggatcgc gctcgcctcg gtgctcgagc
      841 gcgtgtcgag aacctgggtg tcctcgttgc cgcagaaggg gcagcgcatg gcgcgctact
      901 ttacgcgtag accgggaaac gcgcgcacag cgcctgcacc tcgccggcga cgcgccgcag
      961 atggccgtcg tccgccggcc ggtcgagcac gtcggcgacc aggccggcga ggatctcggc
     1021 ctccgccacc cggaagccgc gcgtggtcat ggccggcgag ccgatgcgca ctcccgaggt
     1081 gaccgtcggt ttctccgggt cgttggggat ggcgttcttg ttcaccgtga tgtgcgcgcg
     1141 gccgagcgcc gcttccgcat ccttgccggt gatcttcttg ccgcgcaggt cgagcagcat
     1201 catgtgcgac tcggtgcggc ccgacacgat gcgcacgccg cgcgcggtca gcacgcgcgc
     1261 catggcccgg gcgttgtcca gggtctgctg ctggtaggac ttgaattccg gccgcagcgc
     1321 ctcgccgaac gccacggcct tggcggcgat gacgtgcatc agcggcccgc cctgcagccc
     1381 cgggaagatc gccgagtcga tccccttgcc ggtgtcgccc atgccgagca ccagcccgcc
     1441 gcgcgggccg cgcagcgtct tgtgcgtggt gctggtgacg tagtgcgcca cgccgatggg
     1501 cgagggatac aggcccaccg cgaccatgcc ggcatagtgc gcgatgtcgg ccatcagcag
     1561 cgcgccgcag cggtcggcga tgtcgcggaa gcgccgccag tcgatgcgca gcgaataggc
     1621 cgaggcgccg gcgacgatga gcctgggccg gtgctccagc gcgagctgct cggcggcgtc
     1681 gtagtcgatc tcctccttcg cgtccaggcc gtaggagatg aactggtaca gcttgcccga
     1741 gaggttgacg ctcgacccgt gcgtcaggtg cccgccgtgc gcgagcgaca tgccgagcac
     1801 cttgtcgccc ggcttgagca cggtgacgta cacggcctgg ttggcctgcg agcccgaatg
     1861 cggctgcacg ttcgcccagt cggcgccgaa cagtttgcgc gcgcgcgcga tggcgagcgt
     1921 ctcggcgagg tcgacgtatt cgcagccgcc gtagtagcgc ttgccgggat agccctcggc
     1981 gtacttgttg gtgagccgcg agccctgcgc cgcgagcacc gcggggctca cgtagttctc
     2041 cgaggcgatc agctcgacgt ggtcctcctg gcgccggttc tcctgggcga tggcgtccca
     2101 gagctcggga tcggtgcggg cgagcaggtc ggaagcggac atggggcggg ccggagggcg
     2161 aagcccgcga ttctacaggc tagtccccct cgggctccac gcggtagcgc agcgtatggt
     2221 cgccgtggcg cgcggcggtg ttgcccaggc cgatccagcc gcggtcggag agcaccgcct
     2281 cctcgcggcg caggaggatc tcggcgctgt tgccgtccga cagcaggtag gtgccgttgg
     2341 aggacaggtc ctgcagcacg aaatggcctt cgcgcgcgaa cagcctggcg tgcacgcgcg
     2401 acgcgaagtg gctgtcgacc accaggtcgt tgaccttgtc gcggccgatg cgcatgccgc
     2461 tgtcgctgga gcgcaccacg cgcacctccc cctggctggt gatcttcagc accgtttcgc
     2521 gcgagcgcgt cagcttggcg cgcgccaggt tggtctcggt gacatcgggg tcggagcgcc
     2581 acagcacgct gcaggcgatg atctcctcgt gccggccgcg cacgcggatc gggaacatct
     2641 gccggcagcg gccgcgcagg ccgggcggca gcgcgtccac ggtcagctgg gtggtcagca
     2701 cctgctcggc gttggccagg gccaccagcc gcgcgcacac gttgacggta tcgccgaaca
     2761 cgtcgccctc ggacagcacg accgggccga aggtgaagcc gaccttgatg cccaggcgct
     2821 cggcggcgtg cgcgggcgag gtggcctcca tctgcatgtc gcatgcggac tggaacgcgg
     2881 cgcccgcgtc cggatacagg cacaccatgc cgtcgccgag cttcttcacc acggcgccac
     2941 cgtggcgcga agccgcctta tccaggagtc cgaacagcga attgatcagg gccagcgccc
     3001 gcgtatcgcc gagcgattcg tagatcctgg tgctgtcgac gacgtcggcg aacagtaccg
     3061 cgcgctgcgc agtctccacg ctgtgatttt cccccggcga tagagcggcg agtttacacc
     3121 gcgagcggcg gtgcgaggtc ctccggtcgg tctatgtcgc gcagcgctcc cgggtcgccc
     3181 acgggcacct tcaccagctg cgccgcgtgc gccgcgagca cgcttttcgc gcctgcgtcg
     3241 ccggccagtg cctcaagctc cgcgcgcagg ctgccggcga accccaccgg atgtccgcgc
     3301 cgcgcgcgcc agtagggcgc cgccaggatg gcgccctgcg ccagcgcatc gcgcaccgcg
     3361 gcgatggtgc tcgggcgcac gaacggcatg tcggcgagag ccaccaggta gccgtcggcc
     3421 gcgccggccg cgcgcgccgc gcaggcgagg ctcgcgccca tgccctcgcc ggcccgctcg
     3481 cacaccacca cttcgcagcc ctcgccggcc agcgcgcgcg ccgtcgcatc ggcgccggga
     3541 cgcaccaccg ccagcacgcg cggcagctcc gccctgaggt ggcgcgccgc ctgcacggcg
     3601 atcgcgacgc cgtgcggcag cgcatgctgc agcttgtcgg aaccgaaacg ggtggcggcg
     3661 cctgcggcga gcagcaggcc ggcgaacttc aagtcttgat ttcgcaagcc gaggggtcga
     3721 agcgcgcgtc cttcttcgcc gaccagctcg gctcgctcac gccgtggcgc accgcggtca
     3781 gctcggcgag gatcgccacc gcgatctcgg gcggcgtctt ggagccgatg tacaggccca
     3841 ccgggccgcg cagtcgcgcg atctcggcct cggcgaggtc gaactcgagc aggcgcttgc
     3901 ggcgcgcgtc gttgttcttc ttcgagccga tggcgccgac gtagaacgcg gccgacttga
     3961 ggccc
//
LOCUS       DASRIE010000153         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_161075, whole genome shotgun sequence.
ACCESSION   DASRIE010000153 DASRIE010000000
VERSION     DASRIE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161075"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFB53_06505"
     CDS             complement(<1..1026)
                     /locus_tag="VFB53_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN86418.1"
                     /translation="MIDLATLDAARAEAAQARRRLVEVLEERLGMDPDAFVAHLGSVH
                     RIACIQMAQLREYPPAFDLLPFSEGAERGCVLVRDSAGALLLVCDDPFSLENHAWAEE
                     RIPAAFEWRLAHRRDLAAYLSSHEETLRALDAVRAETGAAGEAGSSIEDLSLKNISEE
                     KSEVVRLVRSTLRDALLQGASDVHMETGPAGLMIKFRVDGILTQAKTLQDPAQAEQAI
                     ARVKVLAELDITERRIPQDGRFKALHREQPIDFRVSIMPSIHGEDAVLRILDRRALNE
                     DETHAQQRQLSLDALGFEPADVKRLRRLSREPYGMLLVTGPTGSGKTTTLYAAITEIN
                     SGQDKIVT"
     gene            complement(1023..>2167)
                     /locus_tag="VFB53_06510"
     CDS             complement(1023..>2167)
                     /locus_tag="VFB53_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN86419.1"
                     /translation="VESHVLQAADEAYARRQLEGRGYTVLQVRGWRGFALRRRERFPV
                     VLFSQELIALLDAGLPLLEAIETLGEKERAPAAKNALSSLALTLREGRPFSYALEKLG
                     DIFPPLYIATVRAAERTSDLQPALARYVAYQAQVDALKRRVVHASIYPALLVGVGALV
                     SFFLLLYVVPRFSLIYEDRAVDLPLLSRLLLSWGQLVEHHGMAIIIALLCSLGAAIWA
                     MRLPAVRSGTQNLLWRLPGAGERMKLYQLARFYRTIGMLLRGGMPVVASLQLGAELLH
                     PALRSRLQTATRAIREGRPVSESMEANGLTTPVALRLLAVGEHGGDMGEMMERIAAFH
                     DDEMARWVDWLTRLVEPLLMAAIGLVIGAIVILMYMPIFELAGAIT"
BASE COUNT          322 a          756 c          781 g          308 t
ORIGIN      
        1 ggtgacgatc ttgtcctggc cggagttgat ctcggtgatc gcggcgtaga gcgtggtggt
       61 cttgccgctg ccggtgggcc cggtgaccag cagcatgccg tagggctcgc gcgacaggcg
      121 ccgcaggcgc ttcacgtcgg ccggctcgaa gccgagcgcg tcgagcgaga gctggcgttg
      181 ctgggcgtgc gtttcatctt cgttcagggc gcgccggtcg aggatgcgca gcaccgcgtc
      241 ctcgccgtgg atgctgggca tgatcgagac gcggaagtcg atcggctgct cgcggtgcag
      301 cgccttgaag cgcccgtcct gcgggatgcg gcgctcggtg atgtcgagct cggccagcac
      361 cttgacgcgc gcgatcgcct gctcggcctg cgccgggtcc tgcagggtct tggcctgcgt
      421 gaggatgccg tcgacgcgga acttgatcat caggccggcg gggccggtct ccatgtgcac
      481 gtcgctcgcg ccctgcagca gggcgtcgcg cagcgtcgag cgcaccaggc ggaccacctc
      541 gctcttttct tccgagatat tcttcagcga caaatcttcg atcgagctgc cggcttcgcc
      601 cgccgcgccg gtctcggcgc gcaccgcgtc gagcgcgcgc agcgtctcct cgtggctcga
      661 gaggtaggcg gcgaggtcgc gccggtgcgc gagccgccac tcgaacgcgg ccgggatgcg
      721 ttcctcggcc caggcgtggt tctcgagcga gaacgggtcg tcgcacacca gcagcagcgc
      781 cccggccgag tcgcgcacca gcacgcagcc gcgctcggcg ccctcggaga acggcagcag
      841 gtcgaaagcc ggggggtact cgcgcagttg cgccatctgg atacaggcaa tcctgtgaac
      901 agaacccagg tgggcgacga acgcatccgg gtccatgccg aggcgctcct cgagcacctc
      961 caccagccgg cggcgcgcct gcgccgcctc ggcgcgcgcc gcgtcgagcg tggcgaggtc
     1021 gatcacgtga tcgccccggc gagctcgaag atcggcatgt acatgaggat cacgatcgcg
     1081 ccgatcacca ggccgatcgc ggccatcagc agcggctcga ccaggcgcgt gagccagtcc
     1141 acccagcgcg ccatctcgtc gtcgtggaac gcggcgatgc gctccatcat ctcgcccatg
     1201 tcgccgccgt gctcgcccac cgcgagcagg cgcagcgcca ccggcgtggt caggccgttc
     1261 gcctccatcg attcggatac cggccgtccc tcgcggatcg cgcgcgtcgc ggtctgcagg
     1321 cggctgcgca gggcggggtg caggagctcg gcgccaagct gcagcgacgc caccaccggc
     1381 atgccgccgc gcagcagcat gccgatggtg cggtagaagc gcgcgagctg gtagagcttc
     1441 atgcgctcgc cggcgcccgg caggcgccag agcaggtttt gcgttcccga cctgacggcg
     1501 ggcagccgca tcgcccagat cgccgcgccc agcgagcaca gcagcgcgat gatgatcgcc
     1561 atgccgtggt gctcgaccag ctggccccac gacagcagca gccgcgagag cagcggcagg
     1621 tcgaccgcgc ggtcctcgta gatcaggctg aagcgcggca cgacatagag cagcaggaag
     1681 aaactcacca gcgcgccgac gccgaccagc agggcggggt agatcgacgc gtgcacgacg
     1741 cggcgcttga gcgcgtcgac ctgcgcctgg taggcgacgt agcgcgcgag cgcgggctgc
     1801 aggtcgctgg tgcgctcggc ggcgcgcacc gtggcgatgt agagcggcgg aaagatgtcg
     1861 cccagcttct ccagggcgta ggagaacggc cggccttcgc gcaaggtgag ggcgaggctg
     1921 ctcaaggcat tcttggcggc gggcgcgcgt tccttttcgc ccagggtctc gatcgcctcg
     1981 agcagcggca gcccggcgtc gagcagcgcg atcagctcct ggctgaacag caccaccggg
     2041 aagcgctcgc gccggcgcag cgcgaagccg cgccagccgc gcacctgcag cacggtatag
     2101 ccgcggcctt cgagctggcg gcgcgcgtac gcctcatccg cggcctgcag cacgtgcgac
     2161 tccacgc
//
LOCUS       DASRIE010000154         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1717311, whole genome shotgun sequence.
ACCESSION   DASRIE010000154 DASRIE010000000
VERSION     DASRIE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717311"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFB53_06515"
     CDS             <1..1090
                     /locus_tag="VFB53_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZN86420.1"
                     /translation="FALETVASLPRGARISFDGRVLSTTPVRARLRYEAASVTEPEAV
                     AVERAGALRWATRLPPDSNPRAVALAQEWRAAAASDLEVLARAIAFLRDGRFLYTLEP
                     PLLGANPVDEFLFTTRAGFCEHFSSAFVVLMRAAGVPARVVTGYQGGERNIVDNILTV
                     RQSDAHAWAEVFLAGRGWVRVDPTAAAVPGRVEAGLARAVPETTTLPLMMRPQLEWLR
                     GVRDRWEATVHQWNVWVLGYNPERQRDLMTSFGMRDADWRALTAVLFTFLGAMTAVLL
                     IWSLQRLVRPDPVQRAWRAFCRKLAARGVERAPHEGPRDFSARAARAVPAAGGSIERI
                     GALYIALRYGAHATQPGVARLRRMVREL"
     gene            1087..2067
                     /gene="mltB"
                     /locus_tag="VFB53_06520"
     CDS             1087..2067
                     /gene="mltB"
                     /locus_tag="VFB53_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZN86421.1"
                     /translation="MIRILIVLLLCVPALARASYAGRPEIEAFVRELAQRHGFVEGEL
                     QRVFSRVQRVEPVLEAIARPAERVRSWEEYRALLITERRIAEGTEFWKKYRRTLERAE
                     RKYGVPPQYVVAIIGVETYYGRNTGSWRVVDALTTLAFDYPPRARFFRNELESYLLMA
                     REAGIDVFSVRGSYAGAIGIPQFMPSSARRYAVDFDGNGVIDLQKSRSDSIGSVANFL
                     KVHGWQRDADVMLHARVTGDAWRAYSDGQFEPKHSMAELRQAGVEFDSAGRPGASGAL
                     IELANAKRPNDYRVGLRNFYVITRYNRSALYAAAVADLARELSRAYDLGK"
     gene            complement(2049..2867)
                     /locus_tag="VFB53_06525"
     CDS             complement(2049..2867)
                     /locus_tag="VFB53_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZN86422.1"
                     /translation="MRVIALVVAAAALGSACSTPGRWQAPVEDRRPSPRAAAKPPVAA
                     APAPAAQAAADSYTVKRGDTLYSIALEHGADYRDVAQWNALDDPSKLRVGQVLHVKPP
                     PAQQGVTLGAAPSGGRVEARPLGAPPEQKVEPKVEPKPALREEPKLSAQALQFIWPAK
                     GKILAGFAEPRQKGIDIEGKTGDPVIAAAAGRVTYVGSGIPGLGKLVVIRHDQGYITV
                     YAHNKDILVKEQQSVTRGQRIAELGSTDADRPKLHFQIRKGSSAVDPLLYLPKS"
     gene            complement(2864..3544)
                     /locus_tag="VFB53_06530"
     CDS             complement(2864..3544)
                     /locus_tag="VFB53_06530"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZN86423.1"
                     /translation="MAREVKNPSGVGMTSQRTRRRMVERLREQGIRDERVLAAMAAVP
                     RHLFVEEALASRAYEDTALPIGFGQTISQPYIVARMTETLLAGRPPGRILEIGTGCGY
                     QAAVLAHLFPEVYSIERIKGLLERARANLLALRLANLRLAHADGHAGLEKAAPFQSII
                     VAAAARSVPEALRRQLAPGGRMVLPLEQFGKRGGAQRLLMVERNRRGFVESELDAVRF
                     VPMEAGKA"
     gene            complement(3529..4272)
                     /gene="surE"
                     /locus_tag="VFB53_06535"
     CDS             complement(3529..4272)
                     /gene="surE"
                     /locus_tag="VFB53_06535"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZN86424.1"
                     /translation="MRILVSNDDGYFAPGITLLAEGLRQLGEVTVVAPERDRSGASNS
                     LTLDRPLTVRRAPNGYFSVNGTPTDCVHIAVSGLLDFMPDVVVSGVNLGANMGDDTIY
                     SGTVAAAAEGYLLGIPSLAVSLAGQSGEHFASAIEVALELVGRLKRAPLREPVLLNVN
                     VPDVAPAMLRGVEVTRLGRRHKAEPVVKLQTPRGDTAYWIGPAGAAADAGPGTDFHAV
                     GASRVSVTPLRMDLTHTAQLPLAKEWLAK"
     gene            complement(4341..4805)
                     /locus_tag="VFB53_06540"
     CDS             complement(4341..4805)
                     /locus_tag="VFB53_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HZN86425.1"
                     /translation="MDTGISPKDRAAIAKGLSRLLADTYVVYLKTHNFHWNVEGPMFQ
                     TLHVMFMEQYTEAWNAIDLVAERIRSLGHYAPGTYREYLELARVKETPGHPQATQMVR
                     LLIEGQEAIVKTAREVLPLAEKADDQPTLDLLTQRMQVHEKNAWMLRSLLKK"
BASE COUNT          676 a         1779 c         1803 g          786 t
ORIGIN      
        1 gttcgcgctc gaaacggtcg ccagcctgcc gcgcggcgcg cgcatcagct tcgacggccg
       61 ggtcctgtcg accaccccgg tgcgtgcgcg cctacgctac gaggcggcct cggtcaccga
      121 gcccgaggcc gtcgcggtcg agcgcgcggg cgcgctgcgc tgggcaacgc gcctgccgcc
      181 cgactccaac ccgcgcgccg tcgcgctggc gcaggaatgg cgcgcagccg cggccagcga
      241 tctcgaggtg ctggcgcgcg ccatcgcctt cctgcgcgac ggccgcttcc tgtacacgct
      301 ggagccgccg ctcctcggtg cgaacccggt cgacgagttc ctgttcacca ccagggccgg
      361 cttctgcgag cacttctcgt cggcgttcgt ggtcctgatg cgcgccgcgg gcgtcccggc
      421 gcgagtggtg accggctacc agggcggcga gcgcaatatc gtcgacaaca tcctcacggt
      481 gcggcagtcc gacgcccacg cctgggccga ggtattcctc gccgggcgcg gctgggtgcg
      541 cgtcgacccg accgccgcgg cggtgcctgg acgggtcgag gccggcctgg cgcgcgcggt
      601 tcccgaaacc accacgttgc cgctgatgat gcggccgcag ctcgaatggc tgcgcggcgt
      661 gcgcgaccgc tgggaagcca cggtgcacca gtggaacgtc tgggtgctgg gctacaaccc
      721 ggagcggcag cgcgacctga tgacgagctt cggcatgcgc gacgccgact ggcgcgcgct
      781 gaccgcggtg ctgttcacgt tcctcggcgc gatgacggcg gtgctgctca tctggtcgct
      841 gcagcggctg gtgcgccccg acccggtgca gcgcgcctgg cgcgccttct gccgcaagct
      901 cgccgcgcgc ggcgtcgagc gcgcgccgca cgagggcccg cgcgacttct cggcgcgcgc
      961 ggcgcgcgcg gtgcccgcgg ccggcggcag catcgagcgc atcggcgcgc tgtatatcgc
     1021 gctgcgctac ggcgcgcacg ccacgcagcc tggggtagcg cggctgcgca gaatggtccg
     1081 ggaactttga tccgcatcct catcgtgctg ctgctctgcg tcccggcgct cgcccgagct
     1141 tcgtacgccg ggcggccgga gatcgaggcg ttcgtgcgcg agctggcgca gcgccatggc
     1201 ttcgtcgaag gcgagctgca gcgcgtattt tcgcgggtgc agcgcgtcga gccggtgctg
     1261 gaagcgatcg cccggccggc ggaacgcgtg cgctcgtggg aggaataccg ggcgctgctg
     1321 atcaccgagc ggcgcatcgc cgagggcacc gagttctgga agaaataccg ccgcacgctc
     1381 gagcgcgccg agcgcaagta cggcgtgccg ccgcagtacg tggtggcgat catcggcgtc
     1441 gagacctact acggccgcaa caccggcagc tggcgcgtgg tcgacgcgct caccaccctc
     1501 gccttcgact acccgccgcg ggcgcgcttc ttccgcaacg agctcgagag ctacctgctg
     1561 atggcgcgcg aggcgggcat cgacgtgttc tcggtgcgcg gctcctacgc cggcgccatc
     1621 ggcatcccgc agttcatgcc gtcgagcgcc cggcgctacg cggtggattt cgacggcaac
     1681 ggcgtgatcg acctgcagaa gagccgctcg gattccatcg gcagcgtcgc caatttcctc
     1741 aaggtccatg gctggcagcg cgacgccgac gtgatgctgc acgcccgcgt gaccggcgac
     1801 gcctggcgcg cctattccga cgggcagttc gagccgaaac actcgatggc cgagctgcgc
     1861 caggcggggg tggagttcga ttcggccggg cgcccgggcg cctccggcgc gctgatcgag
     1921 ctggcgaacg ccaagcggcc aaacgactac cgcgtgggac tgcgcaactt ctacgtgatc
     1981 acgcgctata accgcagcgc gttgtacgcc gccgcggtcg ccgacctcgc ccgggagctg
     2041 tcccgcgcct acgacttggg caagtagagc agcgggtcga ccgcggacga gcccttgcgg
     2101 atctggaagt gaagcttggg ccggtcggcg tcggtgctgc cgagctcggc gatgcgctgg
     2161 ccgcgcgtca cgctctgctg ctccttcacc aggatgtcct tgttgtgcgc gtacaccgtg
     2221 atgtagccct ggtcgtgccg gatcaccacc agcttgccca ggcccggaat gccgctgccg
     2281 acgtaggtga cgcgccccgc cgccgcggcg atcaccgggt cgccggtctt gccctcgatg
     2341 tcgatgccct tctggcgcgg ctcggcgaag ccggcgagga tcttgccctt cgccggccag
     2401 atgaactgca gcgcctgggc gctgagcttg ggctcttcgc gcagcgccgg ttttggctcg
     2461 actttgggtt cgactttctg ttccggtggc gcaccgaggg ggcgcgcttc cacccggccg
     2521 ccgctcggcg cggcaccgag cgtcacgccc tgctgcgccg gcggcggctt cacgtgcagc
     2581 acctggccga cgcgcagctt ggaggggtcg tcgagcgcgt tccattgcgc cacgtcgcgg
     2641 tagtcggcgc cgtgctcgag cgcgatgctg tagagcgtat ccccgcgctt gacggtgtag
     2701 gaatcggccg cggcttgcgc cgcgggcgcg ggcgccgccg cgaccggcgg ctttgcggcg
     2761 gcccgcggcg acggccggcg gtcctccacc ggcgcctgcc agcggcccgg agtgctgcag
     2821 gccgagccca gcgcggcggc tgcaacgacc agagcaatga ccctcacgcc ttgcctgcct
     2881 ccatcgggac gaaacgcacc gcgtcgagct cggattcgac gaagccgcgg cggttgcgct
     2941 cgaccatcag cagccgctgc gccccgccgc gcttaccgaa ctgctccagc ggcagcacca
     3001 ttctgccacc aggagcgagc tggcggcgca gcgcctcggg cacgctgcgc gccgccgccg
     3061 cgacgatgat cgactggaac ggcgcggcct tctccagccc ggcgtggccg tcggcgtgcg
     3121 cgagccgcag gttcgccagg cgcaaggcga gcaggttggc gcgcgcgcgc tcgagcagcc
     3181 ccttgatgcg ctcgatcgag tacacctcgg gaaacaggtg ggccagcacc gccgcctggt
     3241 agccgcagcc ggtgccgatt tcaaggatcc tgccgggcgg ccttccggcg agcagggtct
     3301 cggtcatcct ggccacgata taaggctgcg agatcgtctg gccgaagccg atgggcaggg
     3361 cggtgtcctc gtaggcgcgg ctggccagcg cctcctcgac gaacagatgg cgcggcaccg
     3421 ccgccatcgc cgcgagcacg cgctcgtcgc ggatgccctg ctcgcgcagc cgctcgacca
     3481 tgcggcggcg ggtgcgctgc gaggtcatgc cgacgccgga cgggttcttc acttcgcgag
     3541 ccactccttc gccagcggca gctgcgcggt gtgcgtgagg tccatgcgca gcggcgtcac
     3601 cgacacgcgg ctcgccccga ccgcgtgaaa atcggtcccc gggccggcgt cggcggcggc
     3661 gcccgcagga ccgatccagt aggcggtgtc gccgcgcggc gtttgcagct tcaccaccgg
     3721 ctcggccttg tggcgccggc cgaggcgcgt cacctcgacg ccgcgcagca tggccggcgc
     3781 gacgtcgggc acgttgacgt tgagcagcac cggctcgcgc aacggcgcgc gcttcagccg
     3841 gccgacgagc tcgagcgcga cttcgatcgc gctcgcgaag tgctcgccgc tctggcccgc
     3901 cagcgaaacc gcgagcgagg gaatgccgag caggtagcct tcggcggcgg cggcgacggt
     3961 gccggaatag atcgtgtcgt cgcccatgtt ggcgccgagg ttgaccccgg acaccaccac
     4021 gtcgggcatg aagtcgagca ggccgctgac cgcgatgtgc acgcagtcgg tcggcgtgcc
     4081 gttcaccgag aagtagccgt tcggcgcccg ccgcaccgtg agcggccggt cgagggtgag
     4141 cgagttcgag gccccggagc ggtcgcgttc cggcgccacg accgtcacct cgcccagctg
     4201 gcgcaggccc tcggcgagga gcgtaatgcc cggcgcgaag taaccgtcat cgttggaaac
     4261 caggattctc acgcgctcgg atttgaacag acccgatgcc cgctgtctaa cgggtctgcc
     4321 gctgcttcgg ggactgaagc ctatttcttc agcagcgagc gcagcatcca ggcgttcttc
     4381 tcgtgcacct gcatgcgctg cgtgagcaga tcgagcgtcg gctggtcgtc cgctttctcg
     4441 gcgagcggca gcacctcgcg cgcggtcttc acgatcgcct cctgcccctc gatcagcagc
     4501 ctgaccatct gggtggcttg cgggtggccg ggggtctcct tgacgcgcgc gagctccagg
     4561 tactcgcggt aggtgccggg cgcgtaatgg ccgagcgagc ggatgcgctc ggcgaccagg
     4621 tcgatcgcgt tccaggcctc ggtgtactgc tccatgaaca tcacgtgcag cgtctggaac
     4681 atcgggcctt ccacgttcca gtggaaattg tgcgtcttca gatagacgac gtaggtgtcg
     4741 gcgagcaggc gcgacaggcc cttggcgatc gccgcgcggt ctttgggtga gattccggta
     4801 tccatggcgc cgatcctatt gggctgggta cccggccggc caatggtatt tcccgatgcc
     4861 gggagttgat tgaagctatc gcggcggcgc cgggaagtcg ccgatgggcg gaggatggcc
     4921 gagggtacgc gctgcgttga accacctgag cgactgctgg cgatcggcag gtaccccgcc
     4981 caggcccttt tcgtagatct ctccgagccg gaaggcggcc ttgccatcgc cgctgcgtgc
     5041 cgcc
//
LOCUS       DASRIE010000155        11123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2484256, whole genome shotgun sequence.
ACCESSION   DASRIE010000155 DASRIE010000000
VERSION     DASRIE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484256"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFB53_06545"
     CDS             <1..631
                     /locus_tag="VFB53_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN86426.1"
                     /translation="GIAISLMLATMADRVLRGAMAYKTLLIWPYAVAPAIAGVLWAFL
                     FAPSIGIVTYVLKGWGVDWNWILKGDQAMVLIVVASVWKQISYNFLFFLAGLQSVPRS
                     LIEAAAIDGASPLRRFWTIVFPLLSPTTFFLLVVNIVYAFFDTFGVIDATTQGGPGQS
                     TQILVYKVYHDGVKAADLGSSSAQSVILMFIVIALTVVQFRFVERKVQY"
     gene            631..1479
                     /gene="ugpE"
                     /locus_tag="VFB53_06550"
     CDS             631..1479
                     /gene="ugpE"
                     /locus_tag="VFB53_06550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HZN86427.1"
                     /translation="MVENRPWLTAFSHGVLILGVLAVAFPLYVTFIASTLTLEQIMQV
                     PMQLTPGDQFVENYSQVLTAGSTKGSKAAASSMLFNSLVMALVIPLGKMAISVISAFA
                     IVYFRFPLRMFFFWMIFVTLMLPVEVRIIPTFKVIADLGLLNTYAGLTVPLIASATAT
                     FLFRQFFMTIPDELAEAARVDGASPMRFFWDVLLPLSKTTMAALFVIQFIYGWNQYLW
                     PLLITTDESMYTTVIGIKRMIVGSDSANEWNLIMATAILAMLPPALVVMLMQKWFVKG
                     LVETEK"
     gene            1490..2488
                     /locus_tag="VFB53_06555"
     CDS             1490..2488
                     /locus_tag="VFB53_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HZN86428.1"
                     /translation="MAAVSLRDVRKSYTPELQVIHGVSMEIKDGEFIVILGPSGCGKS
                     TLLRMVAGLETITAGEIAIGGRVVNQLEPKDRDIAMVFQNYALYPHMSVYDNMAYGLR
                     IRGLAKDEIDTRVQKAAGILELKKFLDRKPRALSGGQRQRVAMGRAIVREPKVFLFDE
                     PLSNLDAKLRVQMRGELQALHRRLGTTSLFVTHDQVEAMTLAQRMIVMNAGRAEQIGA
                     PLEVYARPATTFVAGFIGSPPMNLIPKDGRLLGVRPEHLEPCAASEALLTIDVDLIEP
                     LGADIIVYGRIGDGESARVAARLPADAKAQAGKLAVRYAPQNAHWFDPGTGKRIEQ"
     gene            2499..2966
                     /locus_tag="VFB53_06560"
     CDS             2499..2966
                     /locus_tag="VFB53_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HZN86429.1"
                     /translation="MKLNARLLRRGMGWWPPFLGAGIRVRGLTEDFREAVVELRFGRL
                     NRNYVGTHFGGSLYSMTDPFFAIMLMRNLGGEYLVWDKSGSIEYVAPGRGTVRARFVL
                     TEQRLAEIRSQAAGGEKVLPEFQVEVRHAEDDSVVALVRKTLYVRLKPRHRPA"
     gene            2963..3682
                     /gene="ugpQ"
                     /locus_tag="VFB53_06565"
     CDS             2963..3682
                     /gene="ugpQ"
                     /locus_tag="VFB53_06565"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZN86430.1"
                     /translation="MTRWPYPKVLAHRGGGKHAPENTLGAMRLGAQLGFQGVEFDVML
                     AGDGTPVLMHDETLERTTSGRGRVPELAYAQLAALDAGAGEKIPRFEDAARLCRALGL
                     WANVEIKPAQGYERRTGEAVARMAAEFWRGAPLQPVLSSFSPVALLAARQAEPQLPRG
                     LLVGRLPLRWREMLRDLECVALHANYRVLNARVVDEAHRAGAAVLAWTVNDLRSARKL
                     LGWGVDCLVTDALQRIRPDTA"
     gene            complement(3679..4221)
                     /locus_tag="VFB53_06570"
     CDS             complement(3679..4221)
                     /locus_tag="VFB53_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZN86431.1"
                     /translation="MRRCLSSFLLLASCAMPAAAACPALLDRSMDTILEKPQSLCEYA
                     GKVLLVVNTASQCGYTPQYEGLEALYRRYRARGLVVLGFPSNDFGGQEPGSNKEIAAF
                     CVNQYAVEFPMFAKTAVRGAQANPLFADLARASGAAPRWNFHKYLVDRSGRQVASFDT
                     KVAPTDPTLVGAIEKLLEAR"
     gene            complement(4202..5560)
                     /locus_tag="VFB53_06575"
     CDS             complement(4202..5560)
                     /locus_tag="VFB53_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZN86432.1"
                     /translation="MNLPAEIFRAYDIRGIAGRTLTPEVVRAVGRALGAMAPRYAIGR
                     DGRHSGPALAGALAQGINESGADVIDIGMAPTPLTYFAAHHLGCGSCVSVTGSHNPPD
                     YNGLKMVVEGHTLSGEEIQDLRRRIERGGAPAGSGRRTSASVLDAYVARIVGDVKLAR
                     PMRIAVDCGNGVAGMVAARLYRALGCEVEELYCEVDGDFPNHHPDPSHPENLADLITR
                     VKSGGSELGFAFDGDGDRLGVVTKRGAIIFPDRQLMLFAADVLTRNAGAQIIYDVKCT
                     RLLAPWIERHGGRPLIWKTGHSLIKAKLAETGAPLAGEMSGHTFFKERWYGFDDALYA
                     GARLLEILSRNANACAPLEALPDAPSTPELNWRLAEGEPHALVAKLQAAQPFPGARSI
                     LAIDGVRVEYADGFGLARASNTTPVIVLRFEGDTQAALARIQGEFRAALQPLKPDAPL
                     PF"
     gene            complement(5557..6477)
                     /locus_tag="VFB53_06580"
     CDS             complement(5557..6477)
                     /locus_tag="VFB53_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86433.1"
                     /translation="MSPYQAPWWLPGGHLQTVYAALSPAPRIAWRRERWDTPDGDFID
                     LDWAGGGDGPLLALFHGLEGGSSSHYARRIAAEAQRLGWRVVVPHFRGCSGVINRLPR
                     AYHSGDSAELDWILRRLRPAYAAGVSLGGNVLLKWLGEQGAGAAQLLRRAAAVSAPLD
                     LAASGGALDRGVNRRLYTRVFLTTMKAKTYARITLYRTPIDERRLARARTLHEFDDIV
                     TAPLHGFRDADDYWARASSGPWLEHIRVPTLLLNARNDPFLPARALEAAARRAAPAVQ
                     REFPAGGGHAGFPGRGHWLARRILAFLDAP"
     gene            complement(6474..6908)
                     /locus_tag="VFB53_06585"
     CDS             complement(6474..6908)
                     /locus_tag="VFB53_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN86434.1"
                     /translation="MSARIFPTAQDAEAAFYEALERADLELMMAVWSEDEEIVCVHPG
                     GARLVGQDQVRDSWRQIFAAGARARLHVAQQVAIAGPMFAVHSVHENFSVPGEKRALQ
                     PVLATNVYVRTAAGWRMAVHHGSPAPGAAEPAPAPAPPKTLH"
     gene            complement(6905..7870)
                     /gene="waaF"
                     /locus_tag="VFB53_06590"
     CDS             complement(6905..7870)
                     /gene="waaF"
                     /locus_tag="VFB53_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZN86435.1"
                     /translation="MARLLVVAPNWIGDALMAQPLLARLRARLPQARIDVLAPAWVAA
                     VLRRMPEVAGVIETPFAHGRLALRERWRLGRTLKALGYQQAIVLANTWKSALLPFFAD
                     IPIRSGYVGESRYGLLNLLYRKQPGREPMALHYARLAEAPGAEIALPLPEPRLAVDAQ
                     SAQAARARFGIEGSYAVLCPGAEYGPAKRWPYFAELAQRLALPVVVLGSAQERALGAG
                     IKGRNLAGETTLDEAIELMAGAALVVSNDSGLMHVAAALGRPQVALFGSSSPRHTPPL
                     SAQARVVWLGIECSPCYARVCPLGHFRCMQELSVERVLQEAQALA"
     gene            complement(7874..8065)
                     /locus_tag="VFB53_06595"
     CDS             complement(7874..8065)
                     /locus_tag="VFB53_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZN86436.1"
                     /translation="MSTDAARLVDVTEKDLPLHCPVPGAPLWARHPRVFLDVVHEGEA
                     LCPYCGTRYVYKGAAPRGH"
     gene            complement(8062..9036)
                     /locus_tag="VFB53_06600"
     CDS             complement(8062..9036)
                     /locus_tag="VFB53_06600"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZN86437.1"
                     /translation="MAKAKAAPKAPLSMADRDGWIWHDGKLVPWRSATTHVLTHSLHY
                     GLAVFEGVRAYKTLQGTAIYRLAEHTQRLINSAHIYLMKIPYSKAQLMQAQIDVVRAN
                     GHEACYVRPIAFYGSEKMGVSPVGAKVHVAIAAWPWGAYLGPEALEKGIRVKTSSYAR
                     HHVNVTMARSKMSGTYPNSVLATLEATMHGYDEGLLLDVDGFVAEGAGENIFIVKDGV
                     IVEPELTSALTGITRASVIELAAGLGYRVEARRLTRDDIYIADECFFTGTAAEVTPIR
                     ELDGRVIGAGKAGPVTKKLQKAFFDVVTGKDRKHLHWLTPVPMKKGKA"
     gene            9105..9425
                     /locus_tag="VFB53_06605"
     CDS             9105..9425
                     /locus_tag="VFB53_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN86438.1"
                     /translation="MSVNPQERVFAALASPPRRRVLAFLAQTALSTSDLAARFGMSPP
                     AVSRHLAVLQRAGLVSAERQGQRVLYRLNRDVLLDALAGFASLIDGPLRRATQLPRTP
                     REAI"
     gene            9454..10248
                     /locus_tag="VFB53_06610"
     CDS             9454..10248
                     /locus_tag="VFB53_06610"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HZN86439.1"
                     /translation="MKIRFHLLPLLAGLLLLGGCATSPEDDGLLASTPIVRALARESG
                     DLVEVARFSRMRVGAPLPADWLGWGLRSGKRPTEYRLVDNGGATVLEAYANAAASGFY
                     RRVRVDPLRQPLLEWSWRIERMIPGADLRVGAREDSMARLVVSFHGDPKKLDFEDRAK
                     LRLAKLFAGEPLPYAMLIYVWSNQIPVETALPSPQIDRIRMVVVESGAAQVGKWRKYR
                     RNILEDYRRVFGDEPGDIVAVGMLTDSDNTQQVARSYYGDITLRAP"
     gene            complement(10245..>11123)
                     /locus_tag="VFB53_06615"
     CDS             complement(10245..>11123)
                     /locus_tag="VFB53_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HZN86440.1"
                     /translation="SLTVEKLADHLSELADRVLGATLALCWAQLRTRHREAPRFAVVA
                     YGKLGGKELGYASDLDLIFLYDDADERAPENYARLAQRLNHWLTARTSSGALFDTDLR
                     LRPSGESGLLVSSLEAFARYQREAAWVWEHQALTRARFCAGDAALGARFEALRVQVLQ
                     QPREPRALAREVRAMRERLHAAHPNPSRLFDVKHDGGGMIDLEFIVQFLVLAHAARHA
                     ELAGNLGNIALLGIASRLGLIPADLAERCREAYRELRRVQHALRLDGAQFARVPQAQV
                     AAHASAVRALWALLPG"
BASE COUNT         1566 a         3874 c         4020 g         1663 t
ORIGIN      
        1 cggcatcgcc atctcgctga tgctcgcgac catggcggac cgcgtgctgc gcggcgcgat
       61 ggcctacaag acgctgctga tctggcccta cgcggtggcg ccggcgatcg cgggcgtgct
      121 gtgggcgttc ctgttcgcgc cttcgatcgg catcgtcacc tacgtgctga agggctgggg
      181 cgtggactgg aactggatcc tcaagggcga ccaggccatg gtgctgatcg tggtggcctc
      241 ggtgtggaag cagatcagct acaacttcct gttcttcctc gccggcctgc aatcggtgcc
      301 gcgctcgctc atcgaggcgg cggcgatcga cggcgccagc ccgctgcggc gcttctggac
      361 catcgtcttt ccgctcctct cgcccaccac ctttttcctg ctggtggtga acatcgtcta
      421 cgccttcttc gacaccttcg gcgtgatcga cgccaccacc caggggggac cggggcagtc
      481 gacccagatc ctggtctaca aggtctacca cgacggggtc aaggcggccg atctgggaag
      541 ctcctcggcg cagtcggtga tcctgatgtt catcgtcatc gcgctgacgg tggtgcagtt
      601 ccgcttcgtc gagcgcaagg tgcagtactg atggtcgaga atcgcccctg gctgacggcc
      661 ttctcgcacg gcgtgctgat cctcggcgtg ctggcggtcg cattcccgct ctacgtcact
      721 ttcatcgcct cgacgctgac gctggagcag atcatgcagg tgccgatgca gctcaccccg
      781 ggcgaccagt tcgtcgagaa ctattcgcag gttctgacgg ccggctcgac caagggatcc
      841 aaggcggcgg cctcctcgat gctgttcaac agcctggtga tggcgctggt gatcccgctg
      901 ggcaagatgg cgatctcggt catttccgcg ttcgccatcg tctacttccg ctttccgctg
      961 cgcatgttct tcttctggat gatcttcgtg acgctgatgc tgccggtgga agtgcgcatc
     1021 atcccgacct tcaaggtgat cgccgacctg gggctgctca acacctacgc cggactgacg
     1081 gtgccgctga tcgcctcggc caccgcgacc ttcctgttcc gccagttctt catgaccatc
     1141 ccggacgaac tggcggaggc cgcgcgcgtg gacggcgcct cgccgatgcg cttcttctgg
     1201 gacgtgctgc tgccgctgtc gaagaccacc atggcggccc tgttcgtgat ccagttcatc
     1261 tacggctgga accagtacct gtggccgctg ctcatcacca ccgacgaatc catgtacacc
     1321 acggtgatcg gcatcaagcg catgatcgtc ggcagcgact cggccaacga gtggaacctg
     1381 atcatggcca ccgcgatcct cgcgatgctg ccgccggcgc tggtggtgat gctgatgcag
     1441 aagtggttcg tgaagggact cgtggagacg gaaaagtgag aacagcataa tggccgccgt
     1501 atcgctgagg gacgtgcgca agagctacac gccggagctg caggtgatcc acggcgtgtc
     1561 gatggagatc aaggacggcg agttcatcgt catcctcggc ccctcgggct gcggcaagtc
     1621 cacgctgctg cgcatggtgg cgggcctgga gaccatcacc gcgggcgaga tcgcgatcgg
     1681 cggacgcgtg gtgaaccagc tcgagcccaa ggaccgcgac atcgccatgg tgttccagaa
     1741 ctacgcgctc tatccgcaca tgagcgtgta cgacaacatg gcctatgggc tgcgcatccg
     1801 gggcctggcg aaagacgaga tcgatacccg cgtgcagaag gccgccggca tcctcgagct
     1861 gaaaaaattc ctcgaccgca agccgcgcgc gctctcgggc ggccagcgcc agcgcgtcgc
     1921 gatgggacgc gccatcgtgc gcgagccgaa ggtgttcctg ttcgacgagc ccttgtcgaa
     1981 cctggacgcc aagctgcgcg tgcagatgcg cggcgaactg caggccctgc accggcgcct
     2041 gggcaccacc agcctgttcg tgacccacga ccaggtcgag gcgatgacgc tcgcccagcg
     2101 catgatcgtg atgaacgccg gccgcgccga gcagatcggc gcgccgctcg aggtgtacgc
     2161 caggcccgcc accaccttcg tcgccggctt catcggctcg ccgccgatga acctgatccc
     2221 gaaggacggc aggctgctcg gcgtgcgtcc ggagcatctc gagccctgcg ccgcgtcgga
     2281 ggccctgctg acgatcgacg tggacctgat cgagccgctg ggcgccgaca tcatcgtcta
     2341 tggccgcatc ggcgacggcg aaagcgcgcg ggtggcggcg cgcctgccgg cggacgcgaa
     2401 ggcgcaggcg ggcaagctcg cggtgcgcta cgcgccgcag aacgcgcact ggttcgatcc
     2461 gggcaccggc aagcgcatcg agcagtgagg tttccgccat gaagctgaat gcccgtctgc
     2521 tgcgccgggg catgggctgg tggccgccgt tcctcggcgc cgggatccgc gtgcgcggcc
     2581 tgacggagga ctttcgcgaa gcggtggtgg agctcaggtt cggccgcctc aaccgcaact
     2641 acgtggggac gcatttcggc ggcagcctgt actccatgac ggacccgttc ttcgcgatca
     2701 tgctgatgcg caacctcggc ggcgagtacc tggtgtggga caagtccggc tcgatcgagt
     2761 acgtggcgcc ggggcgcggc acggtgcgcg cccgcttcgt gctgaccgag cagcgcctgg
     2821 ccgagatccg cagccaggcg gcgggcggcg agaaggtgct gcccgaattc caggtcgagg
     2881 tgcggcatgc cgaggacgat tcggtggtcg ccctggtgcg caagacgctc tacgtccgtc
     2941 tcaagccgcg gcaccggccc gcgtgacgcg ctggccctac ccgaaggtcc ttgcgcaccg
     3001 cggcggcggc aagcatgcgc cggagaacac gctcggcgcc atgcgcctgg gcgcgcagct
     3061 cggcttccag ggggtggagt tcgacgtcat gctggcgggc gacggcacgc cggtgctgat
     3121 gcacgacgag acgctggagc gcaccaccag cggccgcggc cgcgtgccgg aactggccta
     3181 tgcgcagctg gcggccctcg atgcgggggc gggcgagaaa atcccgcgct tcgaggatgc
     3241 ggcgcggctg tgccgcgcgc tcggcctgtg ggccaacgtc gagatcaagc ccgcgcaggg
     3301 ctacgagcgg cgcaccggcg aggcggtggc gcgcatggcg gccgaattct ggcgcggcgc
     3361 gccgctgcag ccggtgctgt cgtcgttttc cccggtggcg ctgctggcgg cgcgccaggc
     3421 ggagccgcaa ttgccgcgcg ggctgctggt cgggcgcctg cccctgcgct ggcgcgagat
     3481 gctgcgcgac ctggagtgcg tcgcgctgca cgcgaactac cgggtgctca atgcgcgcgt
     3541 ggtcgacgag gcgcaccgcg ccggggccgc ggtgctggcc tggacggtga acgacctgcg
     3601 cagcgcgcgc aaactgctcg gctggggcgt ggactgcctg gtgaccgacg cgctgcagcg
     3661 catccgtccg gataccgcct agcgcgcctc gagcagcttc tcgatggcgc cgaccagcgt
     3721 cgggtcggtg ggcgcgacct tggtgtcgaa gctcgccacc tgccggccgc tgcgatccac
     3781 caggtacttg tggaagttcc agcgcggcgc ggcgccgctc gcgcgcgcga ggtccgcgaa
     3841 cagcgggttg gcctgcgcgc cgcgcaccgc ggtcttggcg aacatcggga actcgaccgc
     3901 gtactggttg acgcagaacg cggcgatttc cttgttgctg ccgggctcct ggccgccgaa
     3961 gtcgttggac gggaagccga gcaccaccag gccgcgggcg cggtacctgc ggtacagcgc
     4021 ctccagcccc tcgtactgcg gcgtgtagcc gcactggctg gcggtgttca ccaccagcag
     4081 caccttgccg gcgtactcgc acagcgactg cggcttctcc aggatcgtgt ccatgctgcg
     4141 gtcgagcagg gcggggcagg cggcggcagc gggcatggcg cagctcgcga ggaggaggaa
     4201 gctagaaagg cagcggcgca tcgggcttga gcggttgcag ggcggcgcgg aactcgcctt
     4261 ggatgcgcgc cagcgccgcc tgcgtgtcgc cctcgaagcg tagcacgatc acaggcgtgg
     4321 tattggaggc gcgcgccagg ccgaagccgt cggcgtactc gacgcgcacg ccgtcgatgg
     4381 cgaggatgct ccgcgcgccg gggaagggct gcgccgcctg cagcttcgcc accagcgcgt
     4441 gcggctcgcc ttcggcgagc cgccagttga gttccggcgt ggacggggcg tcgggcagcg
     4501 cctcgagcgg ggcgcaggcg ttcgcgttgc gcgacaggat ctccagcagg cgcgcgccgg
     4561 catacagcgc gtcgtcgaag ccgtaccagc gctccttgaa gaaggtgtgg ccggacatct
     4621 cgccggcgag cggcgcgccg gtctcggcca gcttggcttt gatgagcgaa tgcccggtct
     4681 tccagatgag gggccggccg ccatggcgct cgatccaggg cgccagcagg cgcgtgcact
     4741 tcacgtcgta gatgatctgc gccccggcat tgcgcgtcag cacgtccgcc gcgaacagca
     4801 tcaattggcg gtcgggaaag atgattgcgc cgcgcttggt gaccacgccg agccggtcgc
     4861 cgtccccgtc gaaggcgaag cccagctcgc tgccgcctga tttcacgcgg gtgatgaggt
     4921 cggcgaggtt ctccgggtgg gaagggtctg gatggtggtt ggggaagtcg ccgtcgacct
     4981 cgcagtagag ctcctcgact tcgcagccca gcgcgcggta cagccgcgcg gcgaccatgc
     5041 cggcgacgcc gttgccgcag tccaccgcga tgcgcatcgg gcgcgcgagc ttcacgtcgc
     5101 cgacgatgcg cgccacgtag gcatcgagga cgctcgcgct cgtgcgccga ccgctcccgg
     5161 ccggggcgcc gccgcgctcg atgcgccggc gcaggtcctg gatctcctcg cccgacagcg
     5221 tgtgcccctc gaccaccatc ttgaggccgt tgtagtcggg cggattgtgg ctgccggtca
     5281 ccgacacgca gctgccgcag cccaggtggt gcgcggcgaa ataggtcagg ggcgtcggcg
     5341 ccatgccgat gtcgatgacg tcggcgccgg attcgttgat gccctgggcc agagcgcccg
     5401 ccagcgcggg gccggagtgc cgcccgtcgc gcccgatggc gtagcgcggc gccatggcgc
     5461 ccagcgcgcg gccgaccgcg cgtaccacct cgggcgtgag cgtgcgcccg gcgatgccgc
     5521 ggatgtcgta ggcgcggaag atttcggcgg gcaggttcat ggcgcgtcga ggaacgcaag
     5581 aatacgccgg gcgagccaat gtcccctgcc cggaaagccg gcgtgcccgc cgccggcggg
     5641 gaattcgcgc tgtaccgccg gcgcggccct gcgcgccgcc gcctcgagcg cgcgggcggg
     5701 caggaacggg tcgttgcgcg cgttgagcag cagcgtcggc acgcggatgt gctccagcca
     5761 ggggccgctc gaggcccgcg cccagtagtc gtcggcgtcg cggaagccgt gcaggggagc
     5821 agtgacgatg tcgtcgaact cgtgcagggt gcgcgcgcgc gccagccgcc gctcgtcgat
     5881 cggcgtgcgg tacagggtga tgcgcgcgta ggtcttggcc ttcatcgtgg tgaggaacac
     5941 gcgggtgtag agccgccggt tgacgccgcg gtccagcgcg ccgcccgagg ccgccaggtc
     6001 gagcggcgcg gacacggcgg cggcgcgtcg cagcagctgc gccgcgccgg cgccctgctc
     6061 gccgagccat ttcagcagca cgttgccgcc cagcgacacg cccgccgcgt acgcgggccg
     6121 caggcggcgc aggatccagt cgagctccgc gctgtcgccg gagtggtagg cgcgcggcag
     6181 gcggttgatc acgcccgagc agccgcggaa gtgcggcacc accacgcgcc agccgaggcg
     6241 ctgcgcctcg gcggcgatcc tgcgcgcgta gtggctggag gagccgccct ccagcccgtg
     6301 gaacagcgcg agcagcgggc cgtcgccgcc gccggcccag tccaggtcga tgaagtcccc
     6361 gtcgggcgtg tcccagcgct cgcggcgcca ggcgatgcgc ggggccggcg agagcgcggc
     6421 gtagaccgtc tgcaggtggc cgccgggcag ccaccagggc gcctggtagg ggctcaatgc
     6481 agggtcttcg gcggcgccgg cgccggcgcc ggctcggccg cgccgggcgc cggcgagccg
     6541 tggtgcacgg ccatgcgcca gcccgccgcg gtgcgcacgt agacgttggt ggcgagcacc
     6601 ggctgcagcg cgcgcttctc gccgggaacc gagaagttct cgtgcacgct gtgcaccgcg
     6661 aacatcggcc ccgcgatcgc cacctgctgc gcgacgtgca gccgcgcgcg agcgccggcg
     6721 gcgaagatct ggcgccagct gtcgcgcacc tggtcctgcc cgaccaggcg cgcgccgccc
     6781 ggatgcacgc agacgatctc ctcgtcctcc gaccacaccg ccatcatcag ctcgagatcg
     6841 gcgcgctcca gcgcctcgta gaaggcggcc tcggcgtcct gcgcggtggg gaagatgcgc
     6901 gcgctcacgc cagcgcctgc gcttcctgca gcacgcgttc cacgctcagc tcctgcatgc
     6961 agcggaaatg ccccagcggg cagacgcgcg cgtagcaggg gctgcactcg atgccgagcc
     7021 agacgacgcg cgcctgcgcc gagagcggcg gcgtgtgccg cgggctggag gagccgaaca
     7081 gcgcgacctg cgggcggccc agcgccgccg ccacgtgcat caggcccgag tcgttcgaca
     7141 ccacgagcgc cgcgcccgcc atcagctcga tcgcctcgtc gagcgtggtc tcgccggcga
     7201 ggttgcgccc cttgatcccc gcgcccagcg cgcgctcctg cgccgagccg agcaccacca
     7261 cgggcagcgc gaggcgctgc gcgagctcgg cgaaataggg ccagcgcttg gccggcccgt
     7321 actccgcgcc cgggcacagc acggcgtagc ttccctcgat gccgaagcgc gcgcgcgccg
     7381 cctgcgccga ctgcgcgtcc accgccagcc gcggctcggg caggggcagc gcgatctccg
     7441 cgcccggcgc ctcggcgagg cgcgcatagt gcagcgccat cggttcgcgg cccggctgct
     7501 tgcggtacag caggttgagc aggccgtagc gcgactcgcc gacgtagccg ctgcggatcg
     7561 ggatgtcggc gaagaacggc agcagcgccg acttccaggt gttggcgagc acgatggcct
     7621 gctggtagcc gagcgccttg agcgtgcgcc cgaggcgcca gcgctcgcgc agggcgaggc
     7681 ggccgtgcgc gaacggggtc tcgatcacgc cggccacttc gggcatgcgg cgcagcaccg
     7741 ccgccaccca ggcgggggcc agcacgtcga tgcgcgcctg cggcagcctg gcgcgcagcc
     7801 gggcgagcag gggctgcgcc atcagggcgt cgccgatcca gttgggcgcg acgacgagaa
     7861 ggcgcgccaa gcgtcagtgc cctctcggcg ccgcgccctt gtagacgtac ctggtgccgc
     7921 agtacgggca cagcgcctcg ccttcgtgca ccacgtcgag gaacacgcgc gggtgccgcg
     7981 cccacagcgg cgcgcccggg accgggcagt gcagcggcag gtctttctcc gtcacgtcga
     8041 ccagccgcgc ggcatcggtg ctcacgcctt gccctttttc atcggcaccg gggtcagcca
     8101 gtggaggtgc ttgcggtcct tgccggtgac cacgtcgaag aaggccttct gcagcttctt
     8161 ggtcaccggg ccggccttgc cggccccgat cacgcggccg tcgagctcgc ggatcggcgt
     8221 cacttccgcc gcggtgccgg tgaagaagca ctcgtcggcg atgtagatgt cgtcgcgcgt
     8281 caggcgccgc gcctcgaccc ggtagcccag gcccgcggcc agctcgatca ccgaagcgcg
     8341 cgtgatgccg gtgagcgcgg aggtgagctc gggttcgacg atcacgccgt ccttgacgat
     8401 gaagatgttc tcgcccgcgc cctcggcgac gaagccgtcg acgtcgagca gcagcccctc
     8461 gtcgtagccg tgcatggtgg cttcgagggt cgccagcacc gaattcgggt aggtgcccga
     8521 catcttggag cgcgccatgg tgacgttgac gtggtggcgc gcataggacg aggtcttcac
     8581 gcggatgccc ttttccagcg cctcggggcc caggtaggcg ccccagggcc aggcggcgat
     8641 cgccacgtgc accttggcgc ccaccggcga cacgcccatc ttctccgagc cgtagaacgc
     8701 gatcggccgc acgtagcagg cctcgtggcc gttggcgcgc accacgtcga tctgcgcctg
     8761 catcagctgc gccttcgaat acgggatctt catcaggtag atgtgcgcgg aattgatcag
     8821 gcgctgcgtg tgctccgcca ggcggtagat cgccgtgccc tgcagcgtct tgtaggcgcg
     8881 cacgccttcg aacacggcga ggccgtagtg cagcgagtgc gtcaggacgt gggtggtggc
     8941 ggagcgccag ggcaccagct tgccgtcgtg ccagatccag ccgtcgcggt ccgccatcga
     9001 gagcggcgcc ttgggcgccg cctttgcttt tgccatggag cggggtctcc ctgagtgagg
     9061 cgccattcta accacaggcg cgaaaactgc gccatactat taacatgtct gttaatcctc
     9121 aggagcgcgt gttcgccgcg ctcgcctcgc cgccgcggcg ccgggtgctc gccttcctcg
     9181 cgcagacggc gctgtccacc tccgacctgg cggcgcgctt cggcatgagc ccgccggcgg
     9241 tgtcgcgcca cctggcggtg ctgcagcgcg ccgggctggt gtcggcagag cgccagggcc
     9301 agcgcgtgct gtaccgcctg aaccgcgacg tcctgctcga cgcgctggcc ggattcgcgt
     9361 cgctgatcga cgggccgctg cggcgcgcca cgcagctgcc gcgcacgccg cgcgaggcga
     9421 tctagtgcta ggcttcgccg actgggagtc ctggtgaaga tccggttcca cttgctgcca
     9481 ctgctggcgg ggctgctgct gctcggcggc tgcgccacgt cgccggagga cgacggcctg
     9541 ctcgcctcga cgccgatcgt gcgcgcgctg gcgcgcgagt ccggcgacct cgtcgaagtg
     9601 gcgcgcttct cgcgcatgcg ggtgggcgcg ccgctgcccg ccgactggct gggctggggg
     9661 ctgcgctcgg gcaagcggcc gaccgagtac cggctggtgg acaacggcgg cgccacggtg
     9721 ctcgaggcct acgccaacgc ggcggcctcc gggttctacc ggcgcgtgcg cgtcgacccg
     9781 ctgcgccagc cgctgctgga atggagctgg cgcatcgagc gcatgatccc gggcgccgac
     9841 ctgcgcgtcg gcgcgcgcga ggattccatg gcgcgcctgg tggtgtcctt ccacggcgac
     9901 ccgaagaagc tcgacttcga ggaccgcgcc aagctgcgct tggccaagct gttcgccggc
     9961 gagccgctgc cttacgccat gctgatctac gtgtggtcga accagattcc ggtggagacg
    10021 gcgctgccca gcccgcagat cgaccgcatc cgcatggtgg tggtggagag cggcgcggca
    10081 caggtgggca aatggcgcaa gtaccggcgc aacatcctcg aggattaccg gcgcgtgttc
    10141 ggcgacgagc ccggcgacat cgtcgcggtc ggcatgctca ccgattccga caacacgcag
    10201 caggtggcgc gcagctacta cggcgacatc accctgcgcg cgccctagcc gggcagcagg
    10261 gcccagagcg cgcgcaccgc gctcgcgtgc gccgccacct gcgcctgcgg cacgcgcgcg
    10321 aactgcgcgc cgtcgaggcg cagcgcatgc tgcacgcggc gcagctcgcg gtaggcctcg
    10381 cggcagcgtt ccgccaggtc cgcggggatc agtccgagcc gcgacgcgat gccgagcagc
    10441 gcgatgttgc ccaggttgcc ggcaagctcg gcatggcgcg cggcgtgcgc cagcaccagg
    10501 aactgcacga tgaactcgag gtcgatcatg ccgccgccgt cgtgcttgac gtcgaacagg
    10561 cgcgaggggt tcgggtgcgc cgcgtgcagg cgctcgcgca tcgcgcgcac ctcccgcgcc
    10621 agcgcgcgcg gctcgcgcgg ctgctgcagc acctgcacgc gcagcgcctc gaagcgcgcg
    10681 ccgagcgcgg cgtcgccggc gcagaagcgc gcgcgcgtca gcgcctggtg ctcccatacc
    10741 caggcggctt cccgctgata gcgggcaaac gcctccagcg aggacaccag caggccggac
    10801 tcgcccgagg gccgcaggcg caggtcggtg tcgaacagcg cgccgctcga ggtgcgcgcg
    10861 gtgagccagt ggttcagccg ctgcgccagg cgcgcgtagt tctccggcgc gcgctcgtcg
    10921 gcgtcgtcgt agaggaagat caggtcgagg tccgaggcat agccgagctc cttgccgccg
    10981 agcttgccgt aggccaccac cgcgaagcgc ggcgcctccc ggtgacgggt gcgcagctgc
    11041 gcccagcaca gcgccagcgt cgcgccgagc acgcggtcgg cgagctcgga cagatggtcg
    11101 gccagcttct ccaccgtcag gct
//
LOCUS       DASRIE010000156        28605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_402754, whole genome shotgun sequence.
ACCESSION   DASRIE010000156 DASRIE010000000
VERSION     DASRIE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 28605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402754"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFB53_06620"
     CDS             <1..939
                     /locus_tag="VFB53_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86441.1"
                     /translation="ELSHMFFLPAVARWWSWPSHPPIEERIRLAHPRFQQEDYRRTRH
                     AATYRDGRVAVLDGAGNVVKVAGGLGALAALVEAPRPEHVDHARRLLERLPATLKMRL
                     AGPEAAATAILELLAGHGELAGVGRHQALLLIELALPALKQLPQKRRDAFLAEVNRVV
                     EADRKVTLSEFVQATLLRQHLREGAGKPIRTEFSKVEQVGQDAHAVLSLIAHASGGDA
                     AAAHAKGAGVLGIALAAPMPVAELSTARLGEALERMRHLQPFQKPRVLKACLETAAAD
                     GKFRLAEAELVRAVAATLDCPLPPVIGALDPATLAA"
     gene            936..3731
                     /locus_tag="VFB53_06625"
     CDS             936..3731
                     /locus_tag="VFB53_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZN86442.1"
                     /translation="MKVLQAALAAAIDGEVRFDAGARAAYASDASNYRQVPIGVVLPR
                     SAEDVVRAMGLCRAHGAPVLARGGGTSLCGQSVNVAVVFDFSKYMDRVLAIDPAARTA
                     LVEPGAVCDSLRGAAEAHRLTFGPDPATHSRCTLGGMIGNNSCGAHSVMAGKTAENIE
                     ALEVLTYDGARFWCGPTDEAEFRRILALGNRQSEIYAALKALADKYAGLIRDGFPKIK
                     RRVSGYNLDQLLPENGFNVARALVGSEGSCALTLQAKATLVKSPPHRVLLVQGYADIY
                     AAGDAVPEVLAAGPIACEGLDERIVGGLRERRLKLDDLALLPEGRAWLMVEFGADTLE
                     QAIDKASALKGRIVTEPGETARLWAIRETGASATSLNLKGTPGPDPVVGWEDAAVDPL
                     RLGDYLREFQALVDRHGYRTSLYGHFGDGCIHARITFELRTPEGLANWRAFLQAATAL
                     VVKYGGSLSGEHGDGQARAEFLPAMYGPELMQAFREFKRIWDPQNRMNPGKLVDAEGK
                     AIPVDAHLRLGPQYRPVTLQTRLAFTSDVGDGFGRAAEHCIGMGKCRSKLGGTMCPSY
                     RATGEERYSTRGRARLLWEMLQGEVIKDGWQSEEVKEALDWCLACKGCRSDCPTHTDM
                     AAYKAEFLSHYYEKKTRPLAVRALARIGEWAPLAARVPGLANALGALGRPLAGLATDR
                     PLPRVAARSFRSRFAPQGKGERVILFDDTFNGYFRPGTAAAAAKVLAHAGCRVELPRK
                     HLCCGRPYYDAGMLDRAQAALEVALQVLEPGVPVVILEPACLSVFRDELAQLFPGDAR
                     AEALARRTLSLAEFLERRGYAPRRAFGRAILQGHCHQKALAGTGPDVRLLRAAGMEVE
                     APDTGCCGMAGSFGFRQETAAASRRIAELALLPRLRAAAPDAVVVADGFSCREQIESL
                     AGRPAQHLAEVLAAALK"
     gene            3728..4348
                     /locus_tag="VFB53_06630"
     CDS             3728..4348
                     /locus_tag="VFB53_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN86443.1"
                     /translation="MRTVAAAGLLAAAAVAQAQTPGERLLEAIAQDKEPVAAILVLGG
                     KADVHARNARRETPLHLAAERDMRALTRLLLDAGARVRARSASGETALHHAALNADPA
                     CAALLLEAGADPRAQNDHGETALHWAAYGGQLQVARLLLERGADADAANLDGNLALHG
                     AASAGHADMVRLLLPRTRAPASRNLEGKSAQDLARERGHEDVAALF"
     gene            complement(4345..5349)
                     /locus_tag="VFB53_06635"
     CDS             complement(4345..5349)
                     /locus_tag="VFB53_06635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN86444.1"
                     /translation="MSLKLSILDQSPVISGHTPAQAVQATLELARRADELGYRRYWLA
                     EHHAIAALADPCPEVLLARLGAETRRMRVGTGGVLLPYYSAFKVAEVFRMLEALYPGR
                     VDLGLGRAPGGDQRTAHAVGGGQFPSADDFPQRVWELVGHLDGTLPDDHPHKRVRLQP
                     GGTTAPEVWLLGSSDYSGALAAQLGLRFAFAHFINAKGGDAVTRAYRERFQPSAREGA
                     SAALVCVFMICAETDARAERLAASIDLRRLHMALNLDTPVPTLEEAERHVYAEEQRRY
                     VLSQRDRAIIGGPEKCRREATALAERYGAEELMVLTITGDYPSRLRSYELLAASFGLA
                     "
     gene            5406..6176
                     /locus_tag="VFB53_06640"
     CDS             5406..6176
                     /locus_tag="VFB53_06640"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HZN86445.1"
                     /translation="MRYALGLVLACLVTPAQAAQCEARSGPRTTALVELYTSEGCDSC
                     PPADRWLQGLAGRGLAPDRVVPLSLHVNYWDYIGWKDPYAQQRFADRQRRLAQVMRAR
                     IIYTPQVLLQGRDFRGWHGAGFEAAVAAINARAPGARIDLSLRAGGAETFDVTARAEL
                     LDASQGPDAALYLASYENKLVSRVTAGENRGRSLAHDYVVLEWVGPIAFAGGRAEGRR
                     SVPLLPKAVPANSGVAAFVQNRRSGEVLQALMLPACPG"
     gene            6270..7382
                     /locus_tag="VFB53_06645"
     CDS             6270..7382
                     /locus_tag="VFB53_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HZN86446.1"
                     /translation="MLATQIRQKDGIFYFVSYPAKDLLGRVRFISRFYGEGEQIAPSR
                     IAQDDDIAQFISRIERTDHAFQRGLSQAKVRQLRNFYETAVTQPPIPGTVLLFTSETL
                     RYQSAGDGIGHLSEPNQKFLIIDGQHRLAALYFYMLDKPAEAATIRVPCMIFDGRSED
                     FSTEMFVIINSTPTRINKSHLVDLYEKVSWAAPDRKFAARLVDKLYREGDSPLRYRIN
                     RLGGRSQRDKWILQAELFNELHRWVRLEWRKIQVSGGSAKNVDKYYGAIRDFLKAGKD
                     VFGDTWGKDNYMVTKPVTLKAMIRVCTALVREDAEPADGRQARWAKRLAPWGEMVREF
                     REEGFYERFAAKGEVERVARIQRDLARAAGIEVKKG"
     gene            complement(7369..8559)
                     /locus_tag="VFB53_06650"
     CDS             complement(7369..8559)
                     /locus_tag="VFB53_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN86447.1"
                     /translation="MRRPLEGVTIVALEQVIAGPFCTRQLAELGARVIKIERPEAGDQ
                     SRAMDRSVRGLSSHFVWTNRSKESLALDVKHPQAKEVLARLLARADVFVQNLAPGAVE
                     RLGLGAAELRARHPKLVWCGISGYGPSGPYAAKKAYDLLVQCEAGLLSVTGTPEAPAK
                     AGIPVADIAAGMYAMSAILAALLRREREGVGATLDITMFEALGEWMGFPAYFTAYGGS
                     APPRSGAYHATIVPYGPFRAGDGKAVFLSIQNEREFARFCGQVLGDAALAQDARFSSS
                     PARMQNRDAMHAAIDKVFGTLTAAQAEARLEAAGIANARLNTMQEFWEHPQLAARDRW
                     REVGTSAGAVRAPKPPFNLSEFEPRMDEVPALGAHSRSILAELGYREPDIARLAAAGA
                     ISPS"
     gene            complement(8556..9368)
                     /locus_tag="VFB53_06655"
     CDS             complement(8556..9368)
                     /locus_tag="VFB53_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZN86448.1"
                     /translation="MPESTLRAVSEELIELAANGHTAPQFSTRHPALDAELGYRAMRR
                     LHQHRLAIGWKPVGRKIGFTNRSIWPRYGVHQPIWGFVYDQTLVRADRDHAEVALEGL
                     PQPRIEPEICFGLKAPPPRSHDAHAILAAIDWVAHACEIVQCFHPDWKVTLPDCTAAN
                     GLHGRLVLGTQLPVAEIPDLEARLPAARVALRRGKQTIDEGTGANVLDSPLYALAHLV
                     ELLAIQPEFDQLHAGEIVSTGTWTDAHPVKPGETWSTAFKGLGLPGLTVAFR"
     gene            9431..9856
                     /locus_tag="VFB53_06660"
     CDS             9431..9856
                     /locus_tag="VFB53_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZN86449.1"
                     /translation="MEAHLIWLLLGLALVVVELITGTFYLLVLGAAALGGAAAAWAGA
                     AFPAQVITTSVVAGVGLYFVHAYRARNAAQQMAPIDAGQPANFESWVDQPAGLARVRY
                     RGASWEARLEGGEAPQPGAVLYVLAAEGNTLRVTARRPA"
     gene            9885..10886
                     /locus_tag="VFB53_06665"
     CDS             9885..10886
                     /locus_tag="VFB53_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HZN86450.1"
                     /translation="MLAKLIVAVVLAVATGVLTSKPSYAVYVFLIAAVVAFVLEGVRV
                     VPQQSAFVVERFGRFRATLEPGLNFIVPVFDRVAYRHSLKEVPLDVPEQVCITKDNTQ
                     LGVDGILYFQVTDPRLASYGSSNYIAAISQLAQTTLRSEIGKMELDKTFESREEINKQ
                     IVQALDEAGRNWGIKVLRYEIKSLTPPEAILRSMQQQITAEREKRALIAKSEGERQQE
                     INVADGEKQAAVLRSEGEKQAAVNRAQGEATALRLIAEANAAAVRAVGEAISDKGGMD
                     AANLKVAQEYVAAFSELAKQSNTLIVPGNAADIAAMITTAMTVLDKTRQTQAAAKGA"
     gene            10888..11283
                     /locus_tag="VFB53_06670"
     CDS             10888..11283
                     /locus_tag="VFB53_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN86451.1"
                     /translation="MSVIAVNHFTILTDKLPETLAFYEEHLNLKAGARPPFTFPGAWL
                     YADGGRGRDAILHIIAGVDGKRLVKGVIDHMAFSGAGLAQAVARLKAKGIAYELRRLP
                     AYGTWQLFFFDPNGAKVEIDFDPAEAAPA"
     gene            11280..11519
                     /locus_tag="VFB53_06675"
     CDS             11280..11519
                     /locus_tag="VFB53_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86452.1"
                     /translation="MNLVQRVAGAAIGVLLIVVGLVFASVVLAAGAAIALLVWAWLWW
                     RTRDLRRAAQRADGTVIEGEFRVEREVSRLDEPPR"
     gene            complement(11491..13383)
                     /locus_tag="VFB53_06680"
     CDS             complement(11491..13383)
                     /locus_tag="VFB53_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZN86453.1"
                     /translation="MAEYEEEKPATAPTERALKWVVSVVAIAMSAYHMYVAGFGPPEA
                     VIFRGTHLLFALTLIFILYPLKPGGGPLWRLADLALLALGWGFVLHIFFNYEAFTNRI
                     IYIDDLTPWDMFFGWVSILITIEATRRVLGWGLVLTALGFLAYPLFFTTVRTPVLLEQ
                     IYLSTEGIFGSTLGVSASYVMLFVVFGAFMEKSGTGKLFMDFALSLTGHTAGGPGKVS
                     VISSSLFGTVSGSAVANVMVDGPMTIPLMKRSGFRPHFAAAVEAVASTGGQIMPPVMG
                     AAAFVMAEFLAVPYAQVALWALIPAILYYVAVFFAVHFEAKRHGLHGVPRSQLPQLGR
                     VMLERGQLFIPIFMIFIGLIAGYSAPLCALFAALACLPIALLRKTTREGISWRTVFEA
                     LEEGAKNTLSVAVACAVAGIVIAATTITGLGIVFTQVVLELAQNSLVLALALTAIAGI
                     ILGLGLPTTPAYIVMVALLVPAVIKLGAPTPAAHMFAFYFAILSAITPPVALAVFASA
                     SLAKADMWDSGWAAMRVGAAGYIVPFMFVYEPALLMINGWGQVHVSLLALASASLGCI
                     CLAAGLHGYLLANARLWERGLLVAAALLLIAPEWISSVVGLGLLATVAAVQWPRRPSA
                     AARPAG"
     gene            complement(13453..14394)
                     /locus_tag="VFB53_06685"
     CDS             complement(13453..14394)
                     /locus_tag="VFB53_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZN86454.1"
                     /translation="MKIRLSLLALAAGAAFATPAAAQQVTFMTGPQGGSWIPLGGALK
                     GMWEKNVPGLAITQTPGAGISNVRGVDEGKAQIGLANSNTTVLGFQGAAPYPKKVTKV
                     CQLANLYPQYFQVVAVADANVNSFADLKGKSVVTQPKGNTGELLTEQVLKLNGMTYQS
                     LSKINFQASYNDAVSLVKDGHAQVFTLGTTAPASAVMDLASARDVKLVPVDDKTFNAI
                     KQSNAGHNKLIIKAGTYPKQDKDVPVIGYSTHVVVACDLPEATVYGMAKTMAAGVNDM
                     AAVVKAIGGSTPKDMALDIGVPFHKGAVKFYKEAGAM"
     gene            complement(14494..15432)
                     /locus_tag="VFB53_06690"
     CDS             complement(14494..15432)
                     /locus_tag="VFB53_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN86455.1"
                     /translation="MKPEIIATTKIFPRTQERLDREFACHKLHEAADRAAFLKDLAPR
                     ARALATFGPAGADAKLMDALPKLEIISNFGVGVDAIDLAAAKQRGIIVTNTPEVLNDC
                     VADTAMALTVNVMRKFPQAEGYLRTGAWAAKGGTPLTTSLGGKVMGILGLGRIGEAIA
                     RRAQAFGMRIRYHNRNRKDVAYPYDADAVSLAQNSDVLMVVTPGGAATEKLVNAKVLD
                     ALGPEGYLVNIARGSVVDEPVLLKYLQEKKIAGAGLDVFADEPRVPPAFFTLDNAVLL
                     PHVGSATMETRTAMGNLQVDNLLAHFAGKPVLTRVV"
     gene            complement(15429..16196)
                     /locus_tag="VFB53_06695"
     CDS             complement(15429..16196)
                     /locus_tag="VFB53_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HZN86456.1"
                     /translation="MAELPKNAFKRALRAGQHQIGLWSSLSSNYSVEVIAGAGYDWIL
                     LDMEHSPNDLESLLAQLQAAAPYPTHPVVRVPWNDMVTIKRVLDAGAQSLLIPYVQNA
                     AEAQAAVAHTRYPPGGLRGVAGTTRATRFGRIKDYARRAHEEICVLVQLETRPALEEI
                     EKVASVEGVDGIFIGPADLHASMGYPGETANPAVLPLIEESMRRIRKAGKAPGYLSPV
                     EADAKRMLAAGAQFVAVGADVGLLARGAENLLAKFRT"
     gene            complement(16196..17161)
                     /locus_tag="VFB53_06700"
     CDS             complement(16196..17161)
                     /locus_tag="VFB53_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86457.1"
                     /translation="MRPIHAFVAAAALSAFAATAGAQTYPNKPIRVIVPYAAGGTSDI
                     LARQIGPKITEAWGQPVIVENKPGANGNVGADFVAKSAPDGYTMLLADIGALCISPSV
                     FPHLPFDPRKDFAPVVMVSYSPHVLAVHPSVPVNTVKELIDYAKANPGKLNFAISGIG
                     GAPHLAGIEFAQRTGVNWAYIPYKGGSQAVADVVGGQANVLFNGMLATWPSVTGGRLK
                     ALAISSSKRVASAAGTPTVAETLPGFETGSVQGVVAAAGTPREIVAKLNAEFKRVLGN
                     PEIKDKLAAQGTEVRADTPDSFGAFIRNETARWAKVVKESGAKFE"
     gene            complement(17190..18203)
                     /locus_tag="VFB53_06705"
     CDS             complement(17190..18203)
                     /locus_tag="VFB53_06705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86458.1"
                     /translation="MPRTAFVTGATGFVGLNLVEQLLAQGWRVLALHRAGAELKYLSR
                     MAAERVAGDITDARSVLDALPEGIDAVFHVAGDTNLWSRNNAAQDRVNIDGTRNVVHA
                     ALERRARRFLHTSSISAYGLQRGRLDERTPQRGKYSKVNYNRSKHLAEEAVRAGIARG
                     LDAVILNPGAILGPYDTRNYARLVTMVAGGTLPGVPPGALPFCHVREVARAHIAAFER
                     GRTGENYLLGGTDARLLELVQAIGGALGKPVPSRPTPAWVLRILGALGALRGALSGKQ
                     PALTPETALQAARELSCDSAKAMRELGYRAVPLREMVADCVGWLAAEGRLPGVQSARL
                     DRS"
     gene            complement(18196..19200)
                     /locus_tag="VFB53_06710"
     CDS             complement(18196..19200)
                     /locus_tag="VFB53_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HZN86459.1"
                     /translation="MGAALRDCRFLLALTTAAAPVAGHWLGAYWHAFFVFWVVVPLLD
                     RLVGRSAQASGAEEARRLEGNAFFRFVLHAWIPLQLGLIGWGAWLVGTGGVTGFDAVL
                     FALSVGLATGGAGITIAHELGHKRAPLDRLLSRVLLVTVSYGHFTVEHNRGHHVRVAT
                     PEDPASARYGESYWAFLPRTLIDSVRHAWELDRREVLLSSLATLACAAALGAAFGPLA
                     VAFFFGQSAMAVALLEAVNYIEHYGLVRRKLPDGRYERPAAQHAWDAYEWLSNSFLVH
                     LQRHADHHLNPTRPYAALQPQSDSPRLPMGYPGMVPLAFLPPLWFAVMNPRVPKVADA
                     "
     gene            complement(19206..20930)
                     /gene="araD"
                     /locus_tag="VFB53_06715"
     CDS             complement(19206..20930)
                     /gene="araD"
                     /locus_tag="VFB53_06715"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HZN86460.1"
                     /translation="MKKRPEELRSHRWYGVKDLRSFGHRSRTAQMGYDRSDYAGKPVI
                     AIVNTWSDINHCHSHFRQRAEEVKRGVWQAGGFPLEMPAMALAETMQKPTTMMYRNFL
                     AMETEELLRSYPVDGCVLMGGCDKTTPALLMGATSMNLPAIYLPAGPMLNSHWRDQTL
                     GSGSDTWKYWDELRAGRITERDWNDIEDAIARSPGTCMTMGTAATMMSLAEALGFTLP
                     GYSSIPAPDSNHARMATLTGKRIVDMVWEDLKPRDILTAGSFVNAITTLMAMGGSTNA
                     IVHLVAMAGRAGLAFPLERYNEISARTPVIANVRPSGQRYLMEDFFYAGGLRALLWQL
                     RDLLDLSARTVNGRTLGENIEGAKVWNAEVILPRDKPLKASGGIVMLRGSLAPDGAVI
                     KTSAADAKLMQHTGRAVVFEDYNDMAARIDRDDLEVDASSVLVLKNAGPLGGPGFPEW
                     GMLPMPKKLVKQGVKDMVRLSDGRMSGTSYGTCILHVAPEAYVGGPLAFVRNGDLIEL
                     DVAKKALNLKIPEAEMAQRRAAWKRPPPKYERSYGAIFSRHVKQANEGCDFDFLEGTA
                     PTAEPEIH"
     gene            20965..22266
                     /locus_tag="VFB53_06720"
     CDS             20965..22266
                     /locus_tag="VFB53_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN86461.1"
                     /translation="MGRKRPDPRIVRPDDIDPQHDWRRPLQAPGHTQVDFEGRVDFRR
                     LHDYRLARTRAALAGSGLGALLCFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGDPY
                     VWDFGSAARHHRLYAPWLHQDHCRAGLTGMRGAVGADTTLFREAAREIKAILAAEGVA
                     RMPLGVDMVEPPMLFALQKEGIEVRDGQQAMLQAREVKNTDELTLLNMAAAMVDGVYQ
                     DIAAALKPGIKESDIVALATKRLYEMGSDCVEAINAISGERCSPHPHNFTDRLIRPGD
                     QAFFDVIQSYMGYRTCYYRTFSVGSATGPQRTAYRKAREWMDKVIELLKPGMSTDRIA
                     AALPKAQAAGFASEMEAFGLNFCHGLGLGLHERPLISRLNSFREPIELKAGMVFAVET
                     YCPASDGISAARIEEEVILTPKGAQVISLYPAQELPIANPA"
     gene            22286..22690
                     /locus_tag="VFB53_06725"
     CDS             22286..22690
                     /locus_tag="VFB53_06725"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN86462.1"
                     /translation="MQPRPELEALMPYAPAVRVEGPADILFVSGATPSPLYHRHPHVL
                     EEHVHPNDIEGQTRRAMENIRLVLEGEGLGWRHVVKVTKYLTDMRDMDGMVAVLEGYF
                     GDWKPASTTVCVNCLSTPGARVELDMIAVKPR"
     gene            22621..23601
                     /locus_tag="VFB53_06730"
     CDS             22621..23601
                     /locus_tag="VFB53_06730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN86463.1"
                     /translation="MRQLPEHSRRARRARHDRREAKMSAAPTVGWIGAGRMGYAMAER
                     LAKGGADVLVWNRTRAKAEPLARVGARVAGALAELAARDIVFCMVSTWKDVQQVIDAL
                     LAGKAQPRMVIECSSISLEGSAELRRRLAARGVDYLAAPVSGNAKVIKAGKLSFVCSG
                     PRQSFDAALPLLRMIAPAASYVGEGELARIVKICHNVFLGVVTQSLAEVTVLAQKAGV
                     PRHAFLEFLNQSVMGSLFTRYKTPAFVNLDFKVTFTPDLLRKDMDLGLDAARRLEVPL
                     PLAAATRDLIQSMMGRGMKEEDFATLLLQQAQASGIALQPENVEVKDGLS"
     gene            23588..24547
                     /locus_tag="VFB53_06735"
     CDS             23588..24547
                     /locus_tag="VFB53_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86464.1"
                     /translation="MGFPKLLVAALALAASQAFAQNFPTKPVRLVISFTPGSSTDIIG
                     RAVAATLSEVWKQPVVAENRAGAGGTIGSTLVAKAAPDGYTLLANSSAHAANPGIYKN
                     MQYDTLKDFTNLALLGGGPNVLMVSPEAGWTRLRDLVDVAKASPGKLNFSSAGIGSGT
                     HFNLEKLKIATGIDVVHVPYKGTPEAIADTISGRVCCYWAPLNAALPHVNGGKALALA
                     VSSAQRSPLLPNVPSVAEQGYPGFDYTLWVGLWGPAGMPADVAAKINRDVNAALASAE
                     LKGRLSKLGTVPGNLTIPQFTDFVKKEVEETSRILQAAGIKPQ"
     gene            complement(24534..25184)
                     /locus_tag="VFB53_06740"
     CDS             complement(24534..25184)
                     /locus_tag="VFB53_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZN86465.1"
                     /translation="MKKLDRNQIAARVARDIPDGATVNLGIGLPTLVANHLPKDREVV
                     LHSENGILGLGPKPAAGAEDEDLIDAGKNPATALAGASYFHHADSFAMIRGGHIDICV
                     LGAFQVSATGDIANWHTGEPDAIPAVGGAMDLAAGAKQVFVTMEHLTKDGRSKIVERC
                     SYPLTGVRCVTRIYTDLAVIDVRPDGLHVVDIVEGLSFEELQRLSGVPLRRLATAA"
     gene            complement(25181..25861)
                     /locus_tag="VFB53_06745"
     CDS             complement(25181..25861)
                     /locus_tag="VFB53_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HZN86466.1"
                     /translation="MIDKFIDSAAAALADVPDGATVMIGGFGNAGMPAELIDALIAQG
                     ARDLTVVNNNAGNADAGLAALIGAGRVRRIICSFPRQTDSWHFDKAYRAGKLELELVP
                     QGTLAERIRAAGAGIGAFYTPTAFGTRLAEGKETRRIGERDYVLEFPIHADFALIKAD
                     RADRWGNLAYRMTARNFGPIMAAAATCTVAQVRETVALGALDPEVVVTPGIFVRRVVR
                     IAPLRRAA"
     gene            25903..26979
                     /locus_tag="VFB53_06750"
     CDS             25903..26979
                     /locus_tag="VFB53_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HZN86467.1"
                     /translation="MATQRVFKPAPLARAIEAIVAAGGSEPREAQLVADNLVTANLTG
                     HDSHGIGMIPRYVDSLQEGGLAPNRHPAVKFDGGALVALDGQAGYGQVIGMEATAIAI
                     ERAKAHGVAVMALANSHHLCRIGQWAEQAVAEGLVSISFVNVISRSIVAPFGGSDARF
                     GTNPCTIGIPLAGQAPFILDMATSGVAQGKVRVAHNKGEKLPPGLLLDDRGHPTLEAK
                     WGVIEPFGALTTFGLHKGYGLAVVCELLGGALSGGGTWHTADRSKKRVLNGMLSILID
                     PQKLGTAEGFAREAREFIDWVKRSPPAPGHDRVRIAGDPERETRAKREKEGIAVDANT
                     WSEIHAAAAKVGVAGETIDRLAKG"
     gene            27166..27615
                     /gene="mraZ"
                     /locus_tag="VFB53_06755"
     CDS             27166..27615
                     /gene="mraZ"
                     /locus_tag="VFB53_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZN86468.1"
                     /translation="MSGAFHGATVVTLDAKGRLAIPTRHREALLASAKHVVLTAHPEG
                     CVLLYPSAAWEPVRARAETFPSFHPQASWWKRLLLGFEEHVSPDGAGRILVSGALRLH
                     AKLERDVMLVGQGGYFELWDSGLWNAKLAQALSGPAVPPPGVEDFSL"
     gene            27634..>28605
                     /gene="rsmH"
                     /locus_tag="VFB53_06760"
     CDS             27634..>28605
                     /gene="rsmH"
                     /locus_tag="VFB53_06760"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZN86469.1"
                     /translation="MSAAPLQHRAVLAAEAVAALALRPDGTYVDGTFGRGGHSRLILE
                     QLGPRGRLIAFDRDPQAVAAARQIRDARFSIAHTSFGRLAQALDAQGAGQVQGMLFDL
                     GLSSPQIDDPARGFSFRGDGPLDMRMDPTQGTPAADWLATASEAQLREVIGGYGEERF
                     AKQVAAAIAAARRSAPIRTTRQLADLVGAAVRTREPGQDPATRTFQAVRIFVNRELEE
                     VSLMLPQAVERLAPGGRLAVIAFHSLEDRIVKRFLAACARPALPRDLPVRASEMPQAL
                     LTIAGKPMRPSAAEAAANPRARSAVLRVAARTAAPFDPSLITRIEPDA"
BASE COUNT         4296 a         9969 c        10144 g         4196 t
ORIGIN      
        1 gagctgtcgc acatgttctt cctgccggcg gtggcgcgct ggtggagctg gccctcgcac
       61 ccgccgatcg aggaacgcat ccggctcgcg cacccgcgct tccagcagga agactaccgg
      121 cgcacgcgcc acgccgcgac ctaccgcgac gggcgcgtcg cggtcctcga cggcgcgggc
      181 aacgtggtca aggtggcggg cgggcttggc gcacttgccg cgctggtcga ggccccgcgc
      241 cccgagcacg tggaccacgc gcgccggctc ctcgagcgcc tgccggcgac gttgaaaatg
      301 cgcctggcgg ggcccgaagc cgcggccacg gcgatcctcg agctgctcgc cgggcacggc
      361 gagctcgcgg gggtcgggcg ccaccaggcg ctgctcctca tcgagctcgc gctgcccgcg
      421 ctcaagcagc tgccgcaaaa gcgccgcgac gccttcctcg ccgaagtgaa ccgcgtggtc
      481 gaggccgacc gcaaggtgac gctttccgag ttcgtgcagg ccacgctgct gcggcagcac
      541 ctgcgcgagg gcgcgggcaa gccgatccgc acggaattct ccaaggtcga gcaggtcggt
      601 caggacgcgc acgccgtgct gtcgctgatt gcgcacgctt cgggcggcga cgcggcggca
      661 gcgcacgcga agggcgccgg cgtcctcggc atcgccctgg cggcgccgat gccggtcgcc
      721 gaactgtcca ccgcgcgcct gggcgaggcg ctcgagcgga tgcgccacct gcaaccgttc
      781 cagaaaccgc gcgtgctcaa ggcctgcctg gagaccgccg ccgccgacgg caagtttcgc
      841 ctggcggaag ccgagctggt gcgcgcggtg gcggcgacgc tcgattgccc gctgccgccg
      901 gtgatcggcg cgctcgaccc cgctacactg gcggcatgaa ggttttgcag gccgcgctcg
      961 cggccgcaat cgacggggag gtgcgcttcg acgccggcgc gcgcgccgcg tacgcgtcgg
     1021 atgcgtccaa ctaccgacag gtaccgatcg gcgtggtgct gccgcgctcg gcggaggacg
     1081 tcgtccgcgc gatgggcctg tgccgcgcgc atggcgcgcc ggtcctcgcg cgcggcggcg
     1141 gcacctcgct ctgcgggcag agcgtgaacg tggcggtggt gttcgatttc tccaagtaca
     1201 tggaccgggt gctggcgatc gaccccgccg cgcgcaccgc gctcgtcgag ccgggcgcgg
     1261 tgtgcgacag cctgcgcggc gcggccgagg cgcaccgcct caccttcggc cccgatccgg
     1321 ccacccacag ccgctgcacg ctcggcggca tgatcggcaa caactcctgc ggcgcgcact
     1381 cggtgatggc gggcaagacg gcggagaaca tcgaggcgct ggaagtgctg acctacgacg
     1441 gcgcacgctt ctggtgcggg ccgacggacg aggcggaatt ccggcgcatc ctcgcgctcg
     1501 gcaaccggca gtccgagatc tacgccgcgc tgaaggcgct ggcggacaag tacgccggcc
     1561 tgatccgcga cggcttcccg aagatcaagc gccgcgtctc gggctacaac ctggaccagc
     1621 tgttgcccga gaacggcttc aacgtggcgc gcgcgctggt gggatccgag ggcagctgcg
     1681 cgctcacgct gcaggcgaag gcgacgctgg tgaaaagtcc tccgcaccgc gtcctgctgg
     1741 tgcagggcta cgccgacatc tacgccgcgg gcgacgcggt gcccgaagtc ctggcggccg
     1801 gcccgatcgc ctgcgaaggc ctggacgaga ggatcgtcgg cggcctgcgc gagcgccgcc
     1861 tgaagctgga cgacctcgcg ctgctccccg aaggccgggc ctggctgatg gtcgagttcg
     1921 gcgccgacac gctcgagcag gcgatcgaca aggcgagcgc gctcaagggc cgcatcgtca
     1981 ccgagccggg ggagaccgcg cggctgtggg caatccgcga gaccggcgcg tcggcgacct
     2041 cgctcaacct gaagggcacg ccggggcccg atccggtggt gggctgggag gatgccgcag
     2101 tggacccgct gcgcctgggg gactacctgc gcgagttcca ggccctggtg gaccgccacg
     2161 gctaccgcac ctcgctctac gggcacttcg gcgacggctg catccatgcg cgcatcactt
     2221 tcgaattgcg cacgcccgag gggctggcga actggcgcgc cttcctgcag gcggccacgg
     2281 cgctggtggt gaaatacggc ggctcgctct cgggggagca tggcgacggc caggcgcgcg
     2341 ccgagttcct gccggcgatg tacgggccgg agctgatgca ggcgttccgc gaattcaagc
     2401 gcatctggga cccgcagaac cgcatgaacc cgggcaagct cgtcgacgcc gagggcaagg
     2461 cgattccggt ggacgcgcat ctgcgcctcg ggccgcagta caggccggtg acgctgcaga
     2521 cccggctcgc cttcacgagc gacgtcggcg acggcttcgg ccgcgccgcc gagcactgca
     2581 tcggcatggg caagtgcaga tcgaaactcg gcggcaccat gtgcccgagc tatcgcgcca
     2641 cgggcgagga gcgctactcg acgcgcggcc gcgcccgcct gctgtgggag atgctgcagg
     2701 gcgaggtgat caaggacggc tggcagagcg aggaggtgaa ggaagcgctc gactggtgcc
     2761 tcgcctgcaa gggctgccgc agcgattgcc cgacgcacac cgacatggcc gcctacaagg
     2821 cggagttcct gtcgcactac tacgagaaga agacgcggcc gctggcggtg cgggcgctcg
     2881 cgcgcatcgg ggaatgggcg ccgctcgccg cgcgcgtgcc ggggctggcc aacgccctcg
     2941 gcgccctcgg ccgcccgctc gcgggcctgg cgacggatcg tcccttgccg cgcgtcgcgg
     3001 cgcggagctt ccggtcccgg ttcgcgcccc agggcaaagg cgagcgcgtg atcctgttcg
     3061 acgacacctt caacggctac ttccgtcccg gcaccgcggc tgccgcagcg aaagtgctcg
     3121 cgcatgccgg ctgccgcgtg gagctgccgc gcaagcacct gtgctgcggg cggccgtact
     3181 acgacgcggg catgctggac cgcgcgcagg cggcgctgga ggtcgcgctg caggtgctcg
     3241 agccgggcgt gccggtggtg atcctcgagc ccgcctgcct ctcggtgttc cgcgacgagc
     3301 tcgcgcagct gttccccggc gacgcgcgcg ccgaggcgct cgcgcggcgc acgctgtcgc
     3361 tcgccgagtt cctcgagcgg cgcggctacg cgccgcggcg cgccttcggc cgcgccatcc
     3421 tgcagggcca ctgccaccag aaggcgctcg ccggcaccgg cccggacgtg aggctgttgc
     3481 gcgccgccgg catggaggtc gaggcgcccg acaccggctg ctgcggcatg gcgggatcct
     3541 tcggcttccg gcaggaaacc gcggccgcct cgcgccgcat cgccgagctg gcgctgctgc
     3601 cgcgcctgcg cgccgcggcg cccgacgccg tggtggtggc cgacggcttt tcctgccgcg
     3661 agcagatcga gagcctggcc gggcggccgg cgcagcacct cgccgaggtg ctcgccgccg
     3721 cgctcaagtg aggaccgtcg ccgcggcggg gctgcttgcc gcggccgccg tcgcgcaggc
     3781 gcagacgccc ggcgagcgcc tgctcgaggc gatcgcgcag gacaaggagc cggtcgccgc
     3841 catcctggtg ctgggcggca aggcggacgt ccacgcgcgc aacgccaggc gcgagacccc
     3901 gctgcacctg gcggccgaga gggacatgcg cgcgctgacg cggctgctgc tcgacgcggg
     3961 cgcgcgcgtg cgcgcgcgca gcgccagcgg cgagacggcg ctgcaccacg ccgcgctgaa
     4021 cgccgatccg gcctgcgcgg cgctgctgct cgaggcgggc gccgatcccc gggcgcagaa
     4081 cgaccacggc gagacggcgc tgcactgggc ggcctacggc gggcagctgc aggtggcccg
     4141 gctgctcctc gagcgcggcg ccgatgcgga tgcggcgaac ctcgatggca acctggcgct
     4201 gcacggcgcg gccagcgcgg gccatgccga catggtgcgg ctgctgctgc cgcgcacgcg
     4261 cgcgcccgca tcgaggaatc tggaaggcaa gagcgcgcag gacctggcgc gcgagcgcgg
     4321 ccacgaggac gtggccgcgc tgttctaggc caggccgaag ctcgcggcga gcagctcgta
     4381 ggagcgcagg cggctggggt agtcgcccgt gatggtgagc accatcagct cctcggcgcc
     4441 gtagcgctcg gcaagcgccg tcgcctcgcg gcggcatttc tccggcccgc cgatgatggc
     4501 ccgatcgcgc tgggagagca cgtagcgccg ctgttcctcg gcatacacgt ggcgctccgc
     4561 ttcctccagc gtcggcaccg gcgtgtccag gttgagcgcc atatgcaggc gccgcaggtc
     4621 gatcgaggcc gccaggcgct cggcccgcgc atcggtctcg gcgcagatca tgaacacgca
     4681 gacgagcgcc gcgctcgcgc cctcccgcgc cgaaggctgg aagcgttcgc ggtaggcgcg
     4741 cgtcaccgcg tcgccgccct tggcattgat gaaatgcgcg aaagcaaaac gaaggcccag
     4801 ctgggccgcc agcgccccgc tgtagtccga ggagcccagc agccagacct cgggggccgt
     4861 cgtgccgccg ggctgcaggc gcacccgctt gtgcgggtga tcgtcgggca gcgtgccgtc
     4921 caggtgcccg accagttccc agacgcgctg cgggaagtcg tccgcgctgg ggaactgccc
     4981 gccgcccacc gcgtgcgcgg tacgctggtc gccgcccggg gcgcgcccca ggccgaggtc
     5041 cacgcgcccc gggtagagcg cctcgagcat gcggaacacc tcggccacct tgaaggcgct
     5101 gtagtagggc agcagcaccc cgcccgtgcc cacgcgcatg cgccgcgtct cggcgcccag
     5161 gcgcgcgagc agcacctcgg ggcaggggtc ggcgagcgcg gcgatggcgt ggtgctcggc
     5221 gagccagtag cgccggtagc ccagttcgtc cgcgcggcgc gccagttcca gcgtggcctg
     5281 gaccgcctgg gccggggtgt gccccgagat caccggggac tggtccagga tggagagttt
     5341 cagggacacc gcgcattatc ctaggcggcc cgtaagcagc ggacggcccg tttcgactga
     5401 atcccatgcg ctacgcgctc ggcctcgtcc tggcgtgcct ggtgaccccg gcacaggcgg
     5461 cccaatgcga ggcccgctcc gggccccgga cgaccgccct ggtggagctc tatacctccg
     5521 agggctgcga cagctgcccc ccggcggacc gctggctgca gggcctcgcc gggcggggcc
     5581 ttgccccgga ccgggtggtc ccgctttccc tgcacgtcaa ctactgggac tacatcggct
     5641 ggaaggatcc ctacgcccag cagcggttcg cggaccgcca gcggcgcctg gcgcaggtca
     5701 tgcgggcgcg catcatctac accccgcagg tgctgctgca gggccgggat ttccggggct
     5761 ggcacggcgc cggattcgag gcggccgtgg cggcgatcaa tgcccgagcg cccggggcgc
     5821 ggatcgacct ctcgctccgg gcgggaggcg cagagacgtt cgacgtgacg gcgcgggccg
     5881 agctgctgga cgcctcccag gggcccgacg cagcgctcta cctggccagc tacgagaaca
     5941 agctggtgtc ccgggtgacc gccggtgaga accggggacg gtccctcgcc catgactacg
     6001 tggtgctgga gtgggtgggc ccgatcgcct ttgcgggcgg cagggcggag ggccggcgct
     6061 cggttccgct gctgcccaag gccgtcccgg ccaactccgg ggtggccgcg ttcgtccaga
     6121 accggcgctc cggcgaggtt ctgcaggccc tgatgctccc ggcctgcccc ggctagggcc
     6181 ccgaaaagct tccctgcggg ggtcggccgc cttgggtatt attccggcgg tcttgatcgc
     6241 aggggaaacc ctgcccggag gcagcagctg tgctcgcgac ccagattcgc cagaaggacg
     6301 gaatcttcta tttcgtcagc tatcccgcca aggacctcct gggccgggtg cggttcatca
     6361 gccgcttcta tggcgaaggc gagcaaatcg ccccctcgcg catcgcccag gacgacgaca
     6421 tcgcgcagtt catctcgcgc atcgagcgca ccgaccacgc cttccagcgc ggcctgtcgc
     6481 aggccaaggt gcgccagctg cgcaatttct acgagaccgc ggtgacccag ccgccgatcc
     6541 ccgggacggt gctgctgttc acgtccgaga ccctgcgcta ccagtccgcg ggggacggca
     6601 tcgggcacct ctcggagccg aaccagaagt tcctcatcat cgacggccag caccgcctgg
     6661 cggcgctgta cttctacatg ctggacaagc cggcggaggc cgccaccatc cgcgtgccct
     6721 gcatgatctt cgacggccgc agcgaagact tctccaccga gatgttcgtc atcatcaatt
     6781 ccacgccgac gcgcatcaac aagagccacc tcgtcgacct gtacgagaag gtgtcctggg
     6841 cggcgccgga ccgcaagttc gccgcgcgcc tggtggacaa gctgtaccgc gagggcgaca
     6901 gccccctgcg ctaccgcatc aaccgcctgg gcgggcgcag ccagcgcgac aagtggatcc
     6961 tgcaggcgga gctgttcaac gagctgcacc gctgggtgcg cctcgaatgg cgcaagatcc
     7021 aggtgtcggg cggcagcgcg aagaacgtgg acaagtacta cggcgcgatc cgcgacttcc
     7081 tcaaggcggg gaaggacgtg ttcggcgaca cctggggcaa ggacaactac atggtcacca
     7141 agccggtgac cctgaaggcg atgatccgcg tgtgcaccgc gctggtgcgc gaggatgccg
     7201 agcccgccga cggccgccag gcgcgctggg ccaagcgcct cgcgccctgg ggcgagatgg
     7261 tgcgcgaatt ccgggaagaa ggcttctacg agcgcttcgc cgccaagggc gaggtcgagc
     7321 gcgtggcgcg catccagcgc gacctggcgc gcgccgccgg catcgaggtc aagaagggct
     7381 gatcgcgcct gccgccgcga ggcgcgcgat gtccggctcg cggtatccca gctcggcgag
     7441 gatcgagcgg ctgtgcgcac ccagcgccgg cacctcgtcc atgcgcggct cgaactccga
     7501 caggttgaac ggcggcttgg gcgcgcgcac cgcgcccgcg gaggtgccga cttcgcgcca
     7561 gcggtcgcgc gccgcgagct ggggatgttc ccagaactcc tgcatggtgt tgaggcgcgc
     7621 gttggcgatg ccggccgctt ccagccgcgc ctcggcctgc gccgccgtga gggtgccgaa
     7681 caccttgtcg atcgccgcgt gcatcgcgtc gcggttctgc atgcgcgcgg ggctcgagga
     7741 gaagcgcgca tcctgcgcca gcgcggcgtc gcccagcacc tggccgcaga agcgcgcgaa
     7801 ctcgcgctcg ttctggatgc tgaggaacac cgccttgccg tcgcccgcgc ggaacgggcc
     7861 gtagggcacg atggtggcgt ggtaggcgcc cgagcgcggc ggcgcgctgc cgccgtaggc
     7921 ggtgaagtag gcggggaagc ccatccactc gcccagcgcc tcgaacatgg tgatgtccag
     7981 cgtcgcgccc acgccctcgc gctcgcggcg caggagcgcg gcgaggatcg cgctcatggc
     8041 gtacatgccg gcggcgatat cggccacggg gatgccggcc ttggccgggg cttccggcgt
     8101 gccggtcacc gacagcagtc ccgcctcgca ctgcaccagc aggtcgtagg ccttcttcgc
     8161 ggcgtagggc ccggagggcc cgtagccgga aatcccgcac cacaccagct tcgggtggcg
     8221 cgcacgcagc tcggccgcgc ccaggccgag tcgctccacc gcgccgggcg cgaggttctg
     8281 cacgaagacg tcggcccttg ccagcagccg cgcgaggacc tccttcgcct gcgggtgctt
     8341 cacgtcgagc gccaggcttt ccttggagcg gttggtccac acgaaatgcg acgacagccc
     8401 ccgcacggaa cggtccatgg cgcgcgactg gtcgcccgcc tccgggcgct cgatcttgat
     8461 cacgcgggcg ccgagctcgg cgagctggcg ggtgcagaac ggcccggcga tcacctgctc
     8521 gagcgccacg atcgtgacgc cctccagcgg gcgcctcacc ggaacgcgac cgtcaacccg
     8581 ggaagcccga ggcccttgaa cgccgtgctc caggtttccc ccggcttcac cggatgggcg
     8641 tcggtccagg tgccggtgga gacgatctcg ccggcgtgca actggtcgaa ctcgggctgg
     8701 atggcgagca actccaccag gtgcgcgagc gcgtacagcg ggctgtcgag cacgttggcg
     8761 ccggtgcctt cgtcgatggt ctgcttgccg cggcgcagcg ccacccgcgc cgccgggagc
     8821 cgcgcctcga ggtcggggat ctcggccacc ggcagctggg tgccgagcac cagccggccg
     8881 tgcaggccgt tggccgcggt gcaatcgggc agcgtcacct tccagtccgg gtggaagcac
     8941 tgcacgatct cgcaggcgtg ggccacccag tcgatggcgg cgaggatggc gtgcgcgtcg
     9001 tgcgagcgcg gcggcggcgc cttgagaccg aagcagattt ccggctcgat gcgcggctgc
     9061 ggcagcccct cgagcgcgac ttcggcatgg tcgcgatcgg cgcgcaccag ggtctggtcg
     9121 tacacgaagc cccagatcgg ctggtgcacc ccgtagcgcg gccagatgct gcggttggtg
     9181 aagccgatct tgcgccccac gggcttccag ccgatcgcca ggcggtgctg gtgcaggcgg
     9241 cgcatcgcgc ggtagcccag ctcggcgtcg agcgccgggt ggcgcgtgct gaattgcggc
     9301 gccgtgtgcc cgttggcggc cagctcgatc agctcctcgg agaccgcgcg cagggtggat
     9361 tccggcatgg agaaaattct acctccccgg cgtggttgca ccccgccgtt tcgcgtgcaa
     9421 aatacccgcc atggaagccc atctgatctg gctgctgctc ggcctggcgc tggtcgtcgt
     9481 ggagctgatc accggcacct tttatctgct cgtcctcggc gcggcagcgc tgggcggcgc
     9541 cgctgcggcc tgggccgggg cggcgttccc ggcccaggtg atcaccacca gcgtggtggc
     9601 gggcgtcggc ctgtacttcg tgcacgccta ccgcgcccgc aacgccgcgc agcagatggc
     9661 gccgatcgac gcgggacagc cggcgaattt cgagagctgg gtggaccagc cggcgggatt
     9721 ggcgcgcgtg cgctaccgcg gcgcgagctg ggaggcgcgg ctcgagggcg gcgaggcgcc
     9781 gcagcccggc gcggtgttgt acgtgctcgc cgccgaaggc aatacactga gggtcaccgc
     9841 ccggcgcccc gcctagggcg cgcgcacgct gccgaaggaa aaccatgctc gccaagctga
     9901 tcgtcgccgt agtcctcgcc gtggccaccg gcgtgctgac ctcgaaaccg agctatgccg
     9961 tgtacgtgtt cctcatcgcg gcggtggtgg cgttcgtgct ggagggcgtg cgcgtggtgc
    10021 cgcagcagag cgctttcgtg gtggagcgct tcgggcgctt ccgcgccacc ctcgaaccgg
    10081 gcctcaactt catcgttccg gtgttcgacc gcgtcgccta ccggcattcg ctcaaggagg
    10141 tgccgctcga cgtgcccgag caggtgtgca tcaccaagga caacacgcag ctcggcgtcg
    10201 acgggatcct gtatttccag gtcaccgacc cgcggctcgc gtcctacggc tcgtcgaact
    10261 acatcgccgc gatctcgcag ctggcgcaga ccacgctgcg cagcgagatc ggcaagatgg
    10321 aactggacaa gactttcgag tcgcgcgagg agatcaacaa gcagatcgtg caggcgctgg
    10381 acgaagcggg acgcaactgg ggcatcaagg tgctgcgcta cgagatcaag agcctcacgc
    10441 cgcccgaggc aatcctgcgc tcgatgcagc agcagatcac cgccgagcgc gagaagcgcg
    10501 cgctgatcgc caagtccgag ggcgagcgcc agcaggagat caacgtcgcc gacggcgaga
    10561 agcaggcggc ggtgctgcgc tccgagggcg agaagcaggc cgcggtcaac cgcgcgcaag
    10621 gcgaggccac cgcgctgcgc ctcattgcgg aggccaacgc cgccgcggtg cgcgcggtgg
    10681 gggaggcgat ctccgacaag ggcggcatgg acgcggcgaa cctcaaggtg gcgcaggaat
    10741 acgtcgccgc gttctccgag ctcgccaagc agtccaacac cctgatcgtg ccggggaacg
    10801 ccgccgacat cgcggcgatg atcaccaccg cgatgaccgt gctcgacaag acgcggcaga
    10861 cgcaggccgc ggcgaagggg gcctgagatg tccgtcatcg cggtcaacca cttcaccatc
    10921 ctcaccgaca agctgcccga aacgctcgcc ttctacgagg agcacctcaa cctcaaggcc
    10981 ggcgcgcgcc cgccgttcac ctttccgggc gcctggctgt acgccgacgg cggccgcggc
    11041 agggacgcga tcctgcacat catcgccggc gtggacggca agcgcctggt gaagggcgtc
    11101 atcgaccaca tggccttcag cggcgcgggg ctggcgcagg cggtggcgag gctgaaggcg
    11161 aagggcatcg cctacgagtt gcgccggctg ccggcctacg gcacctggca gctgttcttc
    11221 ttcgacccga acggcgccaa agtcgagatc gatttcgacc cggcggaagc cgcgccggct
    11281 tgaacctggt gcagcgcgtc gcgggcgccg ccatcggcgt cctgctgatc gttgtcggcc
    11341 tggtcttcgc gtccgtggtg ctcgcggcgg gcgcggccat cgcgctcctc gtgtgggcct
    11401 ggctgtggtg gcgcacgcgc gacttgcgcc gcgccgcgca gcgcgccgac ggcacggtga
    11461 tcgaaggcga gttccgcgtc gagcgcgagg tcagccggct ggacgagccg ccgcgctagg
    11521 gcgccgcggc cactgcaccg cggccaccgt cgcgagcagc cccaggccca ccacgctgga
    11581 gatccactcc ggggcgatca gcagcagggc ggcggccacc agcagcccgc gctcccacag
    11641 ccgcgcgttg gccagcaggt agccgtgcag ccccgcggcg aggcagatgc agccgaggct
    11701 tgccgaggcc agggccagca ggctgacgtg cacctgcccc cagccgttga tcatcagcag
    11761 cgccggttcg tagacgaaca tgaagggcac gatgtagccc gccgcgccga cgcgcattgc
    11821 ggcccagccc gagtcccaca tgtcggcctt ggcgaggctc gcagaggcga acaccgccag
    11881 cgccaccggc ggcgtgatcg ccgacaggat ggcgaagtag aaggcgaaca tgtgcgccgc
    11941 cggcgtcggc gcgccgagct tgatcaccgc cggcaccagc agcgccacca tgacgatgta
    12001 ggcgggcgtc gtcggcaggc cgaggcccag gatgatgccg gcgatggcgg tcagcgcgag
    12061 cgccagcacg agcgagttct gcgccagctc gagcaccacc tgcgtgaaca cgatgcccag
    12121 tccggtgatg gtggtcgcgg cgatcacgat gccggccacc gcgcaggcca cggccaccga
    12181 cagcgtgttc ttcgcccctt cctccagcgc ctcgaacacg gtgcgccacg agatgccctc
    12241 gcgcgtggtc ttgcgcagca gggcgatcgg caggcaggcg agggcggcga acagcgcgca
    12301 cagcggagcg ctgtagccgg cgatgaggcc gatgaagatc atgaagatcg ggatgaacag
    12361 ctggccgcgc tcgagcatca cgcggcccag ctgcggcagc tggctgcgcg gcacgccgtg
    12421 caggccgtgg cgcttggcct cgaagtgcac ggcgaagaac accgccacgt agtacaggat
    12481 cgccgggatc agcgcccaca gcgcgacctg ggcgtagggc acggcgagga actcggccat
    12541 cacgaacgcc gccgcgccca tcaccggcgg catgatctgg ccgccggtcg aggccacggc
    12601 ctcgaccgcc gcggcgaagt gcggccggaa gcccgagcgc ttcatcagcg gaatggtcat
    12661 cgggccgtcg accatcacgt tggccaccgc gctgcccgac accgtgccga acaggctcga
    12721 ggaaatcacc gacaccttgc cgggaccgcc agcggtgtgg ccggtgagcg acagcgcgaa
    12781 atccatgaac agcttgccgg tgccgctttt ctccatgaac gcgccgaaca ccacgaacag
    12841 catcacgtag ctcgccgaca cgcccagcgt cgagccgaat atgccctcgg tcgacaggta
    12901 gatctgctcg agcagcaccg gggtccggac ggtggtgaaa aacagcgggt aggcgaggaa
    12961 gcccagcgcg gtgagcacca gcccccagcc gagcacgcgc cgcgtcgcct cgatggtgat
    13021 caggatcgac acccagccga agaacatgtc ccagggcgtg aggtcgtcga tatagatgat
    13081 gcggttggtg aaggcctcgt agttgaagaa gatgtgcagc acgaatcccc agccgagggc
    13141 gagcagcgcg agatcggcga ggcgccacag cggcccgccg ccgggcttga gcgggtagag
    13201 gatgaaaatc agcgtgagcg cgaacagcag gtgcgtgccg cggaagatca ccgcttccgg
    13261 cgggccgaag ccggcgacat acatgtggta ggcggacatc gcgatggcga cgaccgaaac
    13321 cacccacttc agcgcgcgct cggtgggcgc cgtcgccggt ttttcttcct cgtattccgc
    13381 cattccttcc ccctgacgag aaacggggcg gccgtgccgc cccgtcctcc cgcgtccgcg
    13441 gtcaagccgc agttacattg cgccggcttc cttgtagaac ttcaccgcgc ccttgtggaa
    13501 cggcacgccg atgtcgagcg ccatgtcctt gggggtcgag ccgccgatcg ccttcaccac
    13561 cgcggccatg tcgttcacgc cggcggccat ggtcttcgcc atgccgtaca cggtggcctc
    13621 cgggaggtcg caggcgacca ccacgtgcgt cgagtagccg atcaccggca cgtccttgtc
    13681 ctgcttcggg taggtgccgg ccttgatgat cagcttgttg tggccggcgt tggattgctt
    13741 gatggcattg aaggtcttgt cgtccaccgg caccagtttc acgtcgcgcg cgctggcgag
    13801 gtccatcacc gccgaggccg gggccgtggt gccgagggtg aacacctggg catggccgtc
    13861 cttcaccagc gacaccgcgt cgttgtagct cgcctggaaa ttgatcttgg acagcgactg
    13921 gtaggtcatc ccgttcagct tgaggacctg ctcggtgagc agctcgccgg tgttgccctt
    13981 gggctgcgtc accaccgact tgcccttcag gtcggcgaag gaattgacgt tggcgtcggc
    14041 caccgccacc acctggaagt actgcgggta caggttcgcc agctggcaga ccttggtcac
    14101 cttcttcgga tagggcgccg cgccctggaa tccgaggacc gtggtgttcg agttggcgag
    14161 gccgatctgc gccttgccct cgtcgacgcc gcgcacgttg gaaatgccgg caccgggcgt
    14221 ctgcgtgatg gcgaggccgg gaacgttctt ctcccacatg cccttcagcg cgccgccgag
    14281 cgggatccac gagccgccct gcgggccggt catgaacgtg acttgctggg ccgcggctgg
    14341 cgtggcgaaa gcggcgcccg ccgcaagggc gagcagggac agtctgattt tcatttctcc
    14401 tccttcgttg gacttgctga aaggctgcga atgcgccggg gatgatagcg cgagtcacgc
    14461 gccagaggat gctgcgctgc gcaaaaacgg gcgtcagaca acccgcgtga gcaccggttt
    14521 gcccgcgaaa tgggcaagga ggttgtccac ctgcaggttt cccatggcgg tgcgggtttc
    14581 catcgtcgcg ctgcccacgt ggggcagcag cacggcgttg tcgagcgtga aaaacgccgg
    14641 cggcacgcgc ggctcgtcgg cgaacacgtc caggccggcg ccggcgatct tcttctcctg
    14701 caggtatttc agcagcaccg gctcgtcgac caccgagccg cgcgcgatgt tcaccaggta
    14761 gccctcgggc cccagcgcat cgagcacctt cgcgttgacc agtttctcgg tcgcggcgcc
    14821 ccccggcgtc accaccatca gcacgtccga gttctgcgcc agcgacacgg cgtcggcgtc
    14881 gtaggggtag gcgacgtcct tccggttgcg gttgtggtac cggatccgca tgccgaaggc
    14941 ctgcgcgcgc ctcgcgatcg cctcgccgat gcgccccagc ccgaggatgc ccatcacctt
    15001 gccgccgagc gacgtggtga gcggcgtgcc gcccttggcc gcccaggcgc cggtgcgcag
    15061 gtagccttcc gcctgcggga acttgcgcat cacgttgacg gtgagcgcca tcgcggtgtc
    15121 ggcgacgcag tcgttcagca cctcgggggt gttggtgacg atgatgccgc gctgcttcgc
    15181 cgcggcgagg tcgatggcgt ccacgccgac gccgaaattg gagatgattt ccagcttggg
    15241 cagcgcgtcc atcagcttcg catccgcgcc ggccggcccg aaggtggcga gcgcgcgggc
    15301 gcgcggcgcc aggtccttca ggaacgcggc acggtcggcg gcctcgtgca gcttgtggca
    15361 ggcgaattcg cggtccaggc gctcctgggt gcgcgggaag attttcgtgg tggcgatgat
    15421 ctcgggtttc atgtcctgaa ttttgccagc aggttttcgg cgccgcgcgc cagcagcccg
    15481 acatcggcgc ccaccgcgac gaattgcgcg ccggcggcga gcatgcgttt tgcatccgcc
    15541 tccaccggcg agaggtagcc cggcgccttg cccgccttgc ggatgcggcg catcgactcc
    15601 tcgatcagcg gcagcaccgc cggattggcg gtctcgccgg gatagcccat cgaggcgtgc
    15661 aagtcggccg gcccgatgaa gatgccgtcc acgccctcca ccgacgccac cttctcgatc
    15721 tcctccagcg ccggcctggt ctccagctgc accagcacgc agatttcctc gtgcgcgcgc
    15781 ctggcgtagt ccttgatgcg tccgaagcgc gtggcgcgcg tggtgccggc cacgccgcgc
    15841 aggccccccg gcgggtagcg cgtatgggcc accgccgcct gcgcttccgc ggcgttctgc
    15901 acgtagggaa tcagcagcga ctgcgcgccg gcgtcgagca cgcgcttgat ggtcaccatg
    15961 tcgttccacg gcacgcgcac gaccgggtgg gtcgggtagg gcgccgccgc ctgcagctgc
    16021 gcgagcaggc tctccaggtc gttcggcgag tgctccatgt cgagcaggat ccagtcgtag
    16081 cccgccccgg cgatcacctc caccgagtag ttcgaggaca ggctcgacca caggccgatc
    16141 tggtgttgtc ccgcgcgcag cgcgcgcttg aaagcgttct tcggcagctc ggccatcatt
    16201 cgaacttggc gcccgattcc ttcaccacct tcgcccagcg cgccgtctcg ttgcggatga
    16261 aggcgccgaa ggaatccggc gtgtcggcgc gcacttcggt gccctgcgcc gcgagcttgt
    16321 ccttgatctc tgggttgccg aggacgcgct tgaactccgc attcagcttc gcgacgatct
    16381 cgcgcggcgt accggcggcg gcgaccaccc cctgcaccga gccggtctcg aagcccggca
    16441 gcgtctcggc caccgtcggc gtgccggcgg ccgacgccac gcgcttggag ctggagatgg
    16501 cgagcgcctt gagccggccg ccggtgaccg agggccaggt cgccagcatg ccgttgaaca
    16561 gcacgttcgc ctgcccgccg accacgtcgg cgaccgcctg cgagccgccc ttgtagggga
    16621 tgtaggccca gttgacgccg gtgcgctgcg cgaactcgat gccggcgagg tgcggcgcgc
    16681 cgccgatgcc ggaaatcgcg aagttgagct tgcccgggtt ggccttggcg tagtcgatca
    16741 gctccttcac cgtgttgacc ggaaccgagg gatgcaccgc cagcacgtgc ggcgagtagc
    16801 tcaccatcac caccggcgcg aagtccttgc gcgggtcgaa cggcaggtgc ggaaacacgc
    16861 tcgggctgat gcacagcgcg ccgatgtcgg cgagcagcat ggtgtagccg tcgggcgcgc
    16921 tcttggcgac gaagtccgcg ccgacgttgc cgttggcgcc gggcttgttc tccacgatca
    16981 ccggctggcc ccaggcctcg gtgatcttcg gcccgatctg gcgcgccagg atgtcggaag
    17041 tgccgcccgc ggcgtacggc acgatgacgc ggatcggctt gttcgggtag gtttgcgcgc
    17101 ccgcggtggc ggcgaaggcc gacagcgccg cggcggcgac aaaggcgtgg attggacgca
    17161 tggcttgctc ctgggtttga accgtctggt taactgcggt cgaggcgcgc cgattgtacc
    17221 cccggcaagc ggccttcggc agccagccag ccgacgcaat cggcgaccat ttcgcgcagc
    17281 ggcaccgcgc ggtagcccag ctcgcgcatc gccttcgcgc tgtcgcagct cagctcgcgc
    17341 gccgcctgca acgccgtttc gggggtcaag gcgggttgct ttccgctgag cgcgccgcgc
    17401 agcgcgccga gcgcacccag gatgcgcagc acccacgccg gcgtgggccg cgacggcacc
    17461 ggcttgccga gcgcgccgcc gatcgcctgc accagttcga gcaagcgtgc gtccgtgccg
    17521 cccaggaggt agttctcgcc cgtgcggccg cgctcgaagg cggcgatatg cgcacgcgcc
    17581 acttcgcgca cgtggcagaa gggcagcgcg ccgggcggca cgccgggcaa ggttccgccg
    17641 gcgaccatcg tcaccaggcg cgcgtaattg cgcgtgtcgt aggggccgag gatcgcgccc
    17701 gggttgagga tcacggcatc caagccgcgc gcgatgccgg cgcgcacggc ttcctcggcc
    17761 aggtgcttcg agcggttgta gttcaccttg gagtacttgc cgcgctgcgg cgtgcgctcg
    17821 tcgaggcggc cgcgctgcag gccgtaggcg gagatgctcg aggtgtgcag gaagcgccgc
    17881 gcgcgccgct cgagcgccgc atgcaccacg ttcctggtgc cgtcgatgtt gacgcggtcc
    17941 tgcgctgcgt tgtttcgcga ccacaggttc gtgtcgcccg cgacgtggaa cacggcgtcg
    18001 atcccctccg gcagggcgtc cagcacggac ctcgcgtcgg tgatgtcgcc ggcgacgcgc
    18061 tcggccgcca tgcgcgagag gtacttcagc tccgcgccgg cgcgatgcag cgcgagcacg
    18121 cgccagcctt gcgccagcag ctgttcgacc aggttcaggc cgacgaaacc tgtggcgccg
    18181 gtgacgaacg ccgtcctagg catccgccac cttcggcacc cgcggattca tcaccgcgaa
    18241 ccatagcgga ggcaggaacg cgagcggcac cattccgggg tagcccatcg gcagccgcgg
    18301 gctgtccgac tgcggctgca gcgccgcgta gggccgcgtc gggttcaagt ggtggtccgc
    18361 atggcgctgc aggtgcacga ggaaactgtt gctcagccat tcgtaggcgt cccaggcgtg
    18421 ctgcgccgcg ggccgctcgt agcggccgtc gggaagcttg cgccgcacga ggccgtagtg
    18481 ctcgatgtag ttgaccgcct ccagcagcgc taccgccatc gcgctctggc cgaagaagaa
    18541 cgcgaccgcg agcggaccga aggcggcgcc gagcgccgcg gcgcaggcga gcgtcgcgag
    18601 cgaggacagc agcacttcgc gccggtcgag ttcccaggcg tggcgcacag aatcgatgag
    18661 cgtgcgcggc aggaacgccc agtagctctc gccgtagcgc gcgctggccg gatcctcggg
    18721 cgtggccacg cgtacgtggt ggccgcgatt gtgctcgacg gtgaagtggc catagctcac
    18781 ggtgacgagg agcacgcgcg acagcagccg gtcgagcggc gcgcgcttgt gcccgagctc
    18841 gtgcgcgatg gtgatgccgg cgccgccggt ggcgaggccg accgagaggg cgaagaggac
    18901 cgcgtcgaac cccgtcacgc cgccggtgcc gacgagccaa gcgccccagc cgatcaggcc
    18961 gagctgcagc gggatccacg cgtgcagcac gaagcggaag aaggcattgc cttccaggcg
    19021 ccgcgcttcc tccgcgccgg acgcctgcgc gctgcggccg acaaggcgat cgagcagtgg
    19081 aaccacgacc cagaagacga aaaaggcgtg ccagtacgcg cccagccagt ggccggcgac
    19141 cggtgccgcc gcggtcgtca gcgcgaggag gaagcggcag tcccggagcg ccgcgcccat
    19201 ggtcttcagt ggatttccgg ctccgcggtc ggcgcggtgc cctcgaggaa atcgaagtcg
    19261 cagccttcgt tcgcctgctt cacgtggcgc gagaagatgg cgccgtagct gcgctcgtac
    19321 ttgggcggcg ggcgcttcca ggcggccctg cgctgcgcca tctctgcctc cgggattttc
    19381 aggttgagcg ccttcttcgc gacgtcgagc tcgatcaggt cgccgttgcg cacgaaggcg
    19441 agcgggccgc cgacgtaggc ctcgggcgcc acgtgcagga tgcaggtgcc gtagctggtg
    19501 ccgctcatgc ggccgtccga caggcgcacc atgtccttca cgccctgctt caccagcttc
    19561 ttcggcatcg gcagcatgcc ccactcgggg aagcccggcc cgccgagcgg ccccgcgttc
    19621 ttcagcacca gaaccgagga ggcgtcgacc tccagatcgt cgcggtcgat cctcgccgcc
    19681 atgtcgttgt agtcctcgaa caccaccgcg cgcccggtgt gctgcatcag cttcgcgtcg
    19741 gccgcgctgg tcttgatcac cgcgccgtcg ggcgcgagcg agccgcgcag catgacgatg
    19801 ccgccggagg ccttcagcgg cttgtcgcgc ggcaggatca cctcggcgtt ccacaccttg
    19861 gcgccctcga tgttctcgcc cagagtcctg ccgttcaccg tccgcgccga gaggtccagc
    19921 aggtcgcgca gctgccagag cagcgcgcgc aggccgccgg cgtagaagaa atcctccatc
    19981 aggtagcgct ggcccgaggg ccgcacgttg gcgatcaccg gggtcctcgc gctgatctcg
    20041 ttgtagcgct ccagcgggaa cgcgagcccg gcgcgccccg ccatggcgac caggtgcacg
    20101 atggcgttgg tcgagccgcc catcgccatc agcgtggtga tggcgttgac gaaggagccc
    20161 gcggtcagga tatcccgcgg cttcaagtcc tcccacacca tgtcgacgat gcgcttgccg
    20221 gtgagcgtgg ccatgcgcgc gtgattggaa tcgggcgccg ggatcgagga gtagccgggc
    20281 agggtgaagc cgagcgcctc ggcgagcgac atcatggtcg ccgccgtgcc catcgtcatg
    20341 caggtgccgg gcgagcgcgc aatcgcatcc tcgatgtcgt tccagtcacg ctccgtgatg
    20401 cggccggcgc gcagctcgtc ccagtacttc caggtgtcgg atcccgagcc gagcgtctgg
    20461 tcgcgccagt ggctgttcag catcggcccg gcgggcaggt agatcgccgg caggttcatg
    20521 ctggtggcgc ccatcagcag cgccggcgtg gtcttgtcgc agccgcccat cagcacgcag
    20581 ccgtccaccg ggtaggagcg cagcagttcc tcggtctcca tggcgaggaa gttgcggtac
    20641 atcatcgtgg tgggcttctg catggtttcg gcgagggcca tcgccggcat ctccagcggg
    20701 aagccgcccg cctgccacac gccgcgcttc acctcttcgg cgcgctggcg gaaatggctg
    20761 tggcaatggt tgatgtccga ccaggtgttg acgatggcga tcaccggctt gccggcgtag
    20821 tcgctgcggt cgtagcccat ctgcgcggtg cgcgagcggt ggccgaacga gcgcaggtcc
    20881 ttgacgccgt accagcggtg cgaacgcagt tcctcggggc gttttttcat ggcgcgattg
    20941 tatatactcc gcgccggagg agacatggga cgcaagcgcc ccgatccgcg catcgtccgc
    21001 ccggacgaca tcgacccgca gcacgactgg cgccggccgc tgcaggcgcc cggccacacg
    21061 caggtcgatt tcgaggggcg cgtcgatttc cgccgcctgc acgactaccg cctcgcgcgc
    21121 acgcgcgcgg cgctcgccgg ctcgggcctg ggcgctctgc tgtgcttcga ccagcacaac
    21181 atccgctaca ccaccagcac ggtgatcggc gaatgggcgc gcgacaagct cacgcgctat
    21241 tcgctgctca ccggcaacgg cgacccgtac gtctgggact tcggctcggc cgcgcgccac
    21301 caccggctat acgcgccctg gctgcaccag gaccactgcc gcgccgggct gacggggatg
    21361 cgcggcgcgg tgggcgccga cacgacgctg ttccgcgagg cggcgcggga aatcaaggcg
    21421 atcctcgccg ccgagggcgt ggcccggatg ccgctcggcg tggacatggt cgagccgccg
    21481 atgctgttcg cgctgcagaa ggagggcatc gaggtgcgcg acggccagca ggcgatgctg
    21541 caggcgcgcg aggtgaagaa cacggacgaa ctgacgctgc tcaacatggc cgccgcgatg
    21601 gtggacggcg tgtaccagga catcgccgcg gcgctcaagc ccggcatcaa ggaatccgac
    21661 atcgtggcgc tcgccaccaa gcgcctgtac gagatgggct cggattgcgt cgaggcgatc
    21721 aacgcgattt ccggcgagcg ctgctcgccg catccgcaca acttcaccga ccgcctgatc
    21781 cgtcccgggg accaggcgtt cttcgacgtg atccagtcct acatgggcta ccgcacctgc
    21841 tactaccgca ccttcagcgt cggcagcgcc accggcccgc agcgcacggc ctaccgcaag
    21901 gcgcgcgagt ggatggacaa ggtgatcgaa ctcctgaagc ccggcatgag caccgaccgc
    21961 atcgccgccg cgctgccgaa ggcgcaggcg gcgggcttcg ccagcgaaat ggaggccttc
    22021 ggactgaact tctgccacgg gctggggctc gggctgcacg agcgcccgct gatctcgcgc
    22081 ctgaactcct tccgcgagcc gatcgagctg aaggccggca tggtgttcgc ggtggagacc
    22141 tactgcccgg cgagcgacgg catctccgcc gcgcgcatcg aggaggaagt gatcctgacg
    22201 ccgaaaggcg cgcaggtgat ttccctctat cccgcccagg agctgccgat tgccaacccc
    22261 gcctgagggc cgcctgccag cggtggtgca gccgcgtccg gaactggagg cgctgatgcc
    22321 ctatgcgccg gcggtgcgcg tggaagggcc ggccgacatc ctgttcgtct ccggcgccac
    22381 gccgtcgccg ctgtaccacc ggcatccgca cgtgctcgag gagcacgtgc acccgaacga
    22441 catcgagggc cagacgcggc gcgcgatgga gaacatccgc ctggtgctcg agggcgaagg
    22501 gctgggctgg cgccacgtgg tgaaagtcac caagtacctg accgacatgc gcgacatgga
    22561 cggcatggtc gccgtgctgg agggctactt cggcgactgg aagccggcga gtaccacggt
    22621 gtgcgtcaat tgcctgagca ctcccggcgc gcgcgtcgag ctcgacatga tcgccgtgaa
    22681 gccaagatga gcgccgcccc gaccgtcggc tggatcggcg ccggccgcat gggctacgcg
    22741 atggcggagc gcctggccaa gggcggcgcc gacgtgctgg tgtggaaccg cacccgggcg
    22801 aaagccgagc cgctcgccag ggtcggcgcc agggtggcgg gcgcgctggc cgaactcgcc
    22861 gcgcgcgaca tcgtgttctg catggtgtcc acctggaagg acgtgcagca ggtgatcgac
    22921 gctctgctcg ccggcaaggc gcagccgcgc atggtgatcg agtgctcgtc gatctcgctc
    22981 gagggctcgg ccgagctgcg ccggcggctc gccgcgcgcg gcgtcgacta cctcgccgcg
    23041 cccgtgtcgg gcaacgccaa ggtgatcaag gcgggcaagc tctccttcgt gtgctcgggg
    23101 ccgaggcaat ccttcgacgc ggcgctgccg ctgctcagga tgatcgcgcc cgccgcgagc
    23161 tacgtcggcg agggcgagct ggcgcgcatc gtcaagatct gccacaacgt gttcctcggc
    23221 gtggtgacgc agtcgctcgc cgaggtcacc gtgctggcgc agaaggcggg cgtgccgcgc
    23281 cacgccttcc tcgagttcct caaccagagc gtgatgggct cgctgttcac gcgctacaag
    23341 accccggcgt tcgtcaacct ggacttcaag gtgacgttca cgccggacct gctgcgcaag
    23401 gacatggacc tgggcctgga cgcggcgcgc cggctggagg tgccgctgcc gctcgccgcg
    23461 gccacccgcg atctcattca gtctatgatg ggccgtggca tgaaggagga ggatttcgcg
    23521 acgctcctgc tgcagcaggc gcaggcgtcc gggatcgcat tgcaacccga aaacgtggag
    23581 gtcaaagatg ggctttccta agttgctggt cgccgcgctg gcgctcgccg cctcgcaggc
    23641 tttcgcgcag aatttcccca ccaagccggt gcggctggtg atctcgttca ccccgggcag
    23701 ctcgaccgac atcattggcc gcgcggtggc ggccacgctg agcgaggtgt ggaaacagcc
    23761 cgtcgtcgcg gagaatcgcg ccggtgccgg cggcaccatc ggctcgacgc tggtcgccaa
    23821 ggctgcgccc gacggctaca cgctgctcgc caattcctcg gcgcatgcgg cgaacccggg
    23881 catctacaag aacatgcagt acgacacgct gaaggacttc accaacctcg cgctgctcgg
    23941 cggcgggccg aacgtgctga tggtaagccc cgaggcgggc tggaccaggc tgcgcgacct
    24001 cgtcgacgtg gcgaaggcga gccccggcaa gctcaacttc tcctcggccg gcatcggcag
    24061 cggcacgcat ttcaacctgg agaagctgaa gatcgcgacc ggcatcgacg tggtgcacgt
    24121 cccgtacaag ggcacgcccg aggccatcgc cgacaccatc agcggccggg tgtgctgcta
    24181 ctgggcgccg ctcaacgccg cgctgccgca cgtgaacggc ggcaaggcgc tcgcactggc
    24241 ggtgtcctct gcgcaacgct cgccgctgct gcccaacgtg ccgagcgtgg ccgagcaggg
    24301 ctaccccgga ttcgactaca cgctgtgggt cggactgtgg gggccggcgg gcatgcccgc
    24361 ggacgtcgcc gccaagatca accgcgacgt gaacgcggcc ctcgccagcg cggaactcaa
    24421 gggccggctg agcaagctgg gcaccgtgcc gggcaacctg acgatcccgc agttcaccga
    24481 cttcgtgaag aaggaagtcg aggaaaccag caggatcctt caggccgcgg gcatcaagcc
    24541 gcagtagcga ggcgcctgag cggcacgccg ctcaggcgct gcagttcctc gaaggacagc
    24601 ccttcgacga tgtccacgac gtgcaggccg tcggggcgca cgtcgatcac cgcgaggtcg
    24661 gtgtagatgc gcgtgacgca gcgcaccccg gtgagcgggt agctgcaccg ctcgacgatc
    24721 ttgctcctgc cgtccttggt gaggtgctcc atcgtcacga agacctgctt cgcgccggcc
    24781 gcgaggtcca tcgcgccgcc gaccgcggga atcgcatcgg gttcgccggt gtgccagttc
    24841 gcgatgtcgc ccgtggcgga cacctggaag gcgcccagca cgcagatgtc gatgtggccg
    24901 ccgcggatca tggcgaagga atccgcgtgg tggaaatagg aggccccggc aagcgcggtc
    24961 gccggattct tgcctgcgtc gatcaaatcc tcgtcctcgg ctcccgcggc cggcttgggc
    25021 ccaagcccca ggatcccgtt ttcgctgtgc aggacgacct cccggtcctt cggcaggtgg
    25081 tttgccacca gcgtcggcag gccgatgccg aggttgacgg tggcgccatc ggggatgtcg
    25141 cgcgccacgc gcgcggcgat ctggttgcgg tcgagcttct tcatgcggcc ctcctcagcg
    25201 gcgcgatgcg caccacgcgc cgcacgaata tccccggcgt gacgaccacc tccgggtcga
    25261 gtgcgccgag cgccacggtc tcgcgcacct gcgccacggt gcatgtcgcg gctgccgcca
    25321 tgatcgggcc gaaattgcgc gcggtcatgc ggtacgcgag gttgccccag cgatcggcgc
    25381 ggtcggcctt gatcagcgca aagtccgcgt ggatcgggaa ttcgagcacg taatcgcgct
    25441 cgccgatgcg ccgcgtttcc ttgccctcgg cgagccgggt gccgaaggcg gtcggcgtgt
    25501 agaaggcgcc gatgccggcg cccgcggcgc ggatgcgctc ggcgagcgtg ccctgcggca
    25561 ccagttcgag ctccagcttg cccgcgcggt aggccttgtc gaaatgccac gaatcggtct
    25621 ggcgcggaaa cgagcagatg atcctgcgca cgcgcccggc gccgatcagc gcggcgagcc
    25681 ccgcatccgc gttgccggca ttgttgttga ccaccgtgag gtccctggcg ccctgcgcga
    25741 tcagcgcgtc gatcagctcg gcgggcatgc cggcgttgcc gaagccgccg atcatcaccg
    25801 tggcgccgtc gggcacgtcg gcgagggcgg cggcggcgga atcgatgaac ttgtcgatca
    25861 tggcgggcgg ggaaggctgc gggccattat aaactcgcgc ccatggccac ccaacgcgta
    25921 ttcaagccgg cgccgctcgc gcgcgccatc gaggcgatcg tcgccgccgg aggcagcgag
    25981 ccgcgcgaag cgcagctcgt cgccgacaac ctcgtcaccg ccaatctcac cgggcacgac
    26041 tcgcacggca tcggcatgat cccgcgctac gtcgattcgc tgcaggaggg cggcctcgcc
    26101 cccaaccgcc atccggcggt gaagttcgac ggtggcgcgc tggtggcgct cgacggacag
    26161 gccggctacg gccaggtgat cggcatggag gccaccgcca tcgccatcga gcgcgccaag
    26221 gcgcacggcg tggccgtgat ggcgctcgcc aattcgcatc acctgtgccg catcgggcag
    26281 tgggccgagc aggcggtggc cgagggcctg gtgtcgatca gcttcgtcaa cgtcatctcg
    26341 cgctccatcg tcgcgccgtt cggcggcagc gacgcgcgct tcggcaccaa tccctgcacc
    26401 atcggcatcc cgctcgccgg ccaggcgccg ttcatcctcg acatggcgac cagcggcgtg
    26461 gcgcagggca aggtgcgcgt tgcgcacaac aagggcgaga agctgccgcc gggactgctg
    26521 ctcgatgacc ggggacatcc cacgctcgag gcgaagtggg gcgtgatcga gcccttcggc
    26581 gcgctcacca ccttcggcct gcacaagggc tacggcctgg cggtggtgtg cgagctgctc
    26641 ggcggcgcgc tctcgggcgg cggcacctgg cacaccgcgg accgctcgaa aaaacgcgtg
    26701 ctgaacggca tgctcagcat cctcatcgat ccgcagaagc tcggcaccgc ggagggcttt
    26761 gcgcgcgaag cgcgcgagtt catcgactgg gtgaaacgct cgccgccggc cccgggccac
    26821 gatcgcgtgc gcatcgccgg ggacccggag cgcgagacgc gcgcgaagcg cgagaaggag
    26881 ggcatcgccg tcgatgccaa cacctggagc gagatccacg ccgcggccgc aaaggtcggc
    26941 gtggcggggg aaaccatcga ccgcctggcc aagggctagg gctcgtactc ttctgagaag
    27001 gcactttcag tagattttcc aagctactga aagatataaa tatttaattt ccagaatcag
    27061 cctgtaaaaa ctgcgctaag tctttgtcag gcctgaagat ttggcttgac ctgccttttt
    27121 ctttccccta aagtggggaa aagtagaaaa aggtggttaa aagggatgag cggggcattt
    27181 cacggcgcaa cggtggtgac gctggacgcg aaagggcgcc tggctatccc cacgcgccat
    27241 cgcgaggcgc tgctcgccag cgcgaagcac gtggtgctca ccgcacaccc cgagggttgc
    27301 gtgctgctgt atccgagcgc ggcctgggag ccggtgcgcg ccagggcgga gaccttcccg
    27361 agctttcatc cgcaggcgag ctggtggaag cgcctgctgc tcggcttcga ggagcacgtc
    27421 agccccgacg gcgccggccg catcctcgtg tcgggcgcgc tgcgcctgca cgcgaagctc
    27481 gagcgcgacg tgatgctggt cgggcagggc ggctacttcg agctgtggga ttccgggctg
    27541 tggaacgcca agctcgcgca ggcgctctcg gggcccgccg tgccgccgcc cggcgtcgag
    27601 gatttctcgc tgtgagcgcg ggcgcgcccg ccgatgagcg ccgctcccct ccagcaccgc
    27661 gcagttctcg ccgccgaagc agtcgcagcg ctcgcgctgc ggccggacgg cacctatgtc
    27721 gacggcacct tcggccgcgg cggccacagc cggctgatcc tcgagcagct gggcccgcgc
    27781 ggacgcctca tcgcgttcga ccgcgacccg caggcggtgg cggctgcgcg ccagatccgg
    27841 gacgcgcgct tctccatcgc gcacacgtcc ttcggcaggc tcgcgcaggc gctcgacgcg
    27901 cagggcgcgg ggcaggtgca gggaatgctg ttcgatctgg gcctgtcctc gccgcagatc
    27961 gacgatccgg cgcgcggctt ctcgttccgc ggcgacgggc cgctcgacat gcgcatggat
    28021 ccgacccaag ggacgccggc cgccgactgg ctggcgacgg cgagcgaagc gcaactcagg
    28081 gaggtcatcg gggggtatgg ggaagaacgg tttgctaaac aggttgcagc ggcgattgca
    28141 gctgctcgcc ggagcgcgcc catccgcacg acccgccaac ttgccgatct cgtgggtgcg
    28201 gcggtccgca cacgggagcc gggccaggat ccggcgacgc gcacctttca ggctgtacgg
    28261 attttcgtca atcgggagct tgaggaagtg tcgctgatgc tgccgcaggc ggtcgagcgc
    28321 ctggcccccg gcgggcgcct ggcggtgatc gccttccatt cgctggagga ccgcatcgtc
    28381 aagcgcttcc tcgccgcctg cgcgcgcccg gcgctgccac gggatttgcc cgtgcgcgcc
    28441 agcgagatgc cacaagccct cctcacaatc gccggcaagc cgatgcgccc ctccgccgcc
    28501 gaagccgccg ccaatccgcg cgcgcgcagc gccgtgctgc gcgtcgccgc gcgcacggcg
    28561 gccccgtttg acccgtcatt gatcacccga atcgagcccg acgcg
//
LOCUS       DASRIE010000157         6273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2236171, whole genome shotgun sequence.
ACCESSION   DASRIE010000157 DASRIE010000000
VERSION     DASRIE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236171"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFB53_06765"
     CDS             complement(<1..917)
                     /locus_tag="VFB53_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN86470.1"
                     /translation="MKLRQLLAAALLAFSATTQAATLRWGAQNDILTLDPHSQNHATT
                     SAILQHTYEGLTRYSKTYGVEPCLATAWQQISPTQLRFTLRKGVKFHDGSPFTADDVV
                     FSYTRVLQPQGTMQIYVSGVKEVKKVDDFTVDFLLSGPNPVLLRNIADFRIMSKSWSE
                     KHKSQNVQDYKAREETYSSRNANGTGPYILKLWEPDKRTVFAANPNWWGKLEGNVTEI
                     IYTPIKADATRIAALLSGDVDLVTDLPVQDVTRLRGEAKLKVLDGHEVRTIFIGMDQH
                     SAELKYSNVKGKNPFKDMRVRKALNVAVDR"
     gene            complement(939..2123)
                     /locus_tag="VFB53_06770"
     CDS             complement(939..2123)
                     /locus_tag="VFB53_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HZN86471.1"
                     /translation="MKNDPLEMLRLFHAELVHIRRDIHANPELGFEEKRTSQLVADKL
                     AEWGVEVHRGLAKTGVVGVIKGRKDSGRAIGLRADMDCLPMHEVSDVPHKSKVAGRMH
                     ACGHDGHTTMLLGAARYLAQSRNFDGTVNLIFQPAEEGGGGGRVMVEQGLFEKFPVDA
                     VYALHNWPGLPAGKIAVRPGPMMAATDEIKIKVSGRGGHAALPHLAADPVVATAHIIT
                     ALQTIASRNANPIDALVVSVCSMQTSQVGAFNVIPGHVELIGTVRTFSPEMRELAEKR
                     IREIAQSVAQGLGASAEVEYRRGYPSTVNTEKEAVFAAKVGEKVFGKGNVVTDGEPTM
                     GGEDFSYMLQAKPGAYVFLGQGGPQGGCFLHNPGYDFNDEVIPLGAGYLAALVEESLP
                     LK"
     gene            complement(2131..3114)
                     /locus_tag="VFB53_06775"
     CDS             complement(2131..3114)
                     /locus_tag="VFB53_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN86472.1"
                     /translation="MLAFILRRLAQAVLVMLTVGLIAFSLFRFVGDPVLFMLGQDATP
                     EDRERITRLLGMDQPFYVQYAKFVGRAVQGEFGLSLRQVQPVSKLFVERLPATLELSF
                     VAASLALLAGVGMGVYTALRRKSWLTQAMLALSLIGVSLPTFLIGILLILVFAVLLGW
                     LPSFGRGETVALGWWSTGLLTWSGIKALILPSITLGLFQMTLIQRLVRSEMLEVLRTD
                     YIKFARARGLSDRAVHFGHALKNTLVPVITITGLQVGAVIAFAIITETVFQWPGMGLL
                     FIQAVSVADVPVMAAYLCLIALFFVVINLAVDLLYYAVDPRLRIERAAMAH"
     gene            3154..4113
                     /gene="mutY"
                     /locus_tag="VFB53_06780"
     CDS             3154..4113
                     /gene="mutY"
                     /locus_tag="VFB53_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZN86473.1"
                     /translation="MPDFAASLIAWQPRHGRHGLPWQGTRDPYRVWLAEVMLQQTQVA
                     AAIPYYRRFLARFPDLGTLAAASQDEVLRLWSGLGYYARGRNLHAAAQAVAAAGGFPT
                     TARGLAELPGVGRSTAAAIAVSAFGRRAAILDGNAKRVFARCFGVEGDKAQWRLAESL
                     LPLRAVRTYTQALMDLGATVCTRAQPACGRCPVAARCVARREDRIHELPRRRARAPLP
                     LRRATWYVLLRDGRVLLERRPGAGLWGGLWTFPERRPRGLAQLRRLPPLDHGFTHFRL
                     RAQPLLGTVRKAAPGQVWLDLEDAVGAAVPAPVRSVLQSLLAA"
     gene            complement(4099..4683)
                     /locus_tag="VFB53_06785"
     CDS             complement(4099..4683)
                     /locus_tag="VFB53_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZN86474.1"
                     /translation="MSAAADLPLFPLQTVLFPGGRLPLRIFEQRYLEMAKACLRDGAA
                     FGVCLIREGSEVGEPALPEDVGCTARIVEWDMPQLGVLQVVARGERRFRIRERRVQAD
                     GLARAAAEFLAGETDAPLDAADLCARVLRRILDAAPAGLVEAPPRYDSSAWVSARLAE
                     LLPLSGAAKQRLLEMDDARARLEILRRLISSSGK"
     gene            complement(4680..5519)
                     /gene="rapZ"
                     /locus_tag="VFB53_06790"
     CDS             complement(4680..5519)
                     /gene="rapZ"
                     /locus_tag="VFB53_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZN86475.1"
                     /translation="MQLILVSGLSGAGKSIALDVLEDGGYYCVDNLPATLLEDVVRFL
                     GDAGHERVALSVDARSAALAALPEAVARLKAGGADCRLLFLEANPPTLVRRFSETRRR
                     HPLTVEGLTLAEAIERERGLLAGVAELGHRIDTSELQPKVLRNWIRDLLGLGGGTLAL
                     LFESFAFRDGIPLDADWVIDARMLPNPHYDPALRPLTGRDAAVIAYLEGQEPVRRLLE
                     DVRAFLARWLPEVVKENRSYLTVAIGCTGGRHRSVYLAEQLAAAFAGSWRVLVRHRGL
                     AKE"
     gene            complement(5528..5785)
                     /locus_tag="VFB53_06795"
     CDS             complement(5528..5785)
                     /locus_tag="VFB53_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HZN86476.1"
                     /translation="MAAAATLAMGAAQAQDKKPTAQQQRMTQCNKEAAGKKGDERKKF
                     MSECLSSKANTQQDRMTACNKQAAGMKGDERKKFMSGCLKG"
     gene            complement(5833..>6273)
                     /locus_tag="VFB53_06800"
     CDS             complement(5833..>6273)
                     /locus_tag="VFB53_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HZN86477.1"
                     /translation="ELISRGHGLVADDVVEISRLAANVLEGRCPPVLKDFIEVRGLGL
                     LDVRTIFGETAVRPKMRLRLIAHLDKPAPAGNRDIAERLPLAEQSEEILGVTVRKVVI
                     PVAAGRNLAVLLEAAVRNYILQLRGINSSAEFVARQKRAMEAGE"
BASE COUNT          889 a         2205 c         2183 g          996 t
ORIGIN      
        1 cgatcgaccg cgacgttcag cgccttgcgc acgcgcatgt ccttgaacgg gttcttgccc
       61 ttgacgttcg agtacttcag ctcggcgctg tgctggtcca tgccgatgaa gatggtgcgc
      121 acctcatggc cgtccaggac cttgagcttc gcctcgccgc gcaggcgcgt cacgtcctgc
      181 acgggcaggt cggtcaccag gtccacgtcg ccggacagca gcgccgcgat gcgcgtggcg
      241 tcagccttga tgggcgtgta gatgatctcc gtgacattgc cttccagctt gccccaccag
      301 ttcgggttcg cggcgaacac cgtgcgcttg tccggttccc acagcttgag gatgtacgga
      361 ccggtcccgt tggcgttgcg cgacgaatag gtctcttccc gcgccttgta atcctggacg
      421 ttctgcgact tgtgtttctc cgaccaggat ttgctcatga tgcggaagtc ggcgatgttg
      481 cgcagcagga ccgggttggg gccggagagc aggaagtcca cggtgaagtc gtcgaccttc
      541 ttcacttcct tcacgccgga gacgtaaatc tgcatcgtgc cctgcggctg caggacgcgg
      601 gtgtacgaga acaccacgtc gtccgcggta aacgggctgc cgtcgtggaa tttcacgccc
      661 ttgcgcaggg tgaagcgcag ctgcgtcggc gaaatctgct gccacgcggt ggcgaggcag
      721 ggctcgacgc cgtaggtctt cgagtagcgc gtgaggcctt cgtaggtatg ctgcaggatc
      781 gcgctggtcg tggcgtggtt ctgcgaatgc gggtcgaggg tcaggatgtc gttctgcgcc
      841 ccccagcgga gcgtggcggc ctgggtggtg gccgagaagg cgagcagcgc cgctgccagc
      901 agctgtctga gtttcattgc gtcggtctcc tgttgccgtt acttcagtgg cagtgattcc
      961 tcgaccagcg cggccaggta gccggcgccg agcgggatca cctcgtcgtt gaaatcgtaa
     1021 cccggattgt gcaggaagca gccgccctgg gggccgccct gcccgaggaa cacgtaggcg
     1081 cccggcttcg cctgcagcat gtaggagaag tcctccccgc ccatggtcgg ctcgccgtcg
     1141 gtcaccacgt tgcccttgcc gaacaccttc tcgccgacct tggcggcgaa cacggcttcc
     1201 ttctccgtgt tgacggtcga cgggtagccg cgccggtatt ccacctcggc gctcgccccc
     1261 aggccctgcg ccacggactg cgcgatttcc cggatgcgct tttcggccag ctcgcgcatc
     1321 tccgggctga acgtgcgcac cgtgccgatc agctcgacgt ggccggggat gacgttgaat
     1381 gcgccgacct ggctggtctg catcgagcac accgagacca ccagcgcgtc gatcggattg
     1441 gcgttgcggc tggcgatggt ctgcagcgcg gtgatgatgt gcgcggtggc caccaccggg
     1501 tcggccgcca ggtgcggcag cgccgcgtgg ccgcccctgc cgctcacctt gatcttgatc
     1561 tcgtcggtcg ccgccatcat cgggccgggg cgcacggcga tcttgcccgc cggcagcccg
     1621 ggccagttgt gcagggcgta caccgcgtcc accggaaact tctcgaacag gccttgctcc
     1681 accatcacgc gcccgccgcc gccgccttcc tccgcgggct ggaagatcag gttcacggtg
     1741 ccgtcgaaat tgcgcgactg cgcgaggtag cgcgccgcgc cgagcagcat ggtggtgtgg
     1801 ccgtcgtggc cgcaggcgtg catgcgcccg gccaccttcg acttgtgcgg cacgtcgctc
     1861 acctcgtgca tcggcaggca gtccatgtcg gcgcgcaagc cgatggcgcg tccggagtcc
     1921 ttcctgccct tgatgacccc gaccacgccg gtcttcgcga ggccgcggtg cacctcgacg
     1981 ccccattcgg cgagcttgtc ggcgaccagt tgcgaggtgc gcttctcctc gaagccgagt
     2041 tcgggattgg cgtggatgtc gcgccggatg tgcaccagct cggcgtggaa caggcgcagc
     2101 atctcgagag ggtcgttttt catggtttcc tcaatgcgcc atggccgcgc gctcgatccg
     2161 caggcgcgga tcgacggcgt agtacagcag gtccacggcg aggttgatga cgacgaagaa
     2221 tagcgcgatc aggcacagat aggcggccat caccggcacg tcggcgacgc tgaccgcctg
     2281 gatgaacagc aggcccatgc cgggccactg gaacacggtc tcggtgatga tggcgaaggc
     2341 gatcacggcg ccgacctgca ggccggtgat ggtgatcacc ggcaccaggg tgttcttgag
     2401 ggcgtggccg aaatgcaccg cgcggtccga gaggccgcgc gcgcgggcga acttgatgta
     2461 gtcggtgcgc agcacctcca gcatctcgga gcgcaccagc cgctggatca gcgtcatctg
     2521 aaagaggccc agcgtgatcg agggcaggat cagcgccttg atgccgctcc aggtcagcag
     2581 gcccgtgctc caccagccga gggccaccgt ctcgccgcgc ccgaaggagg gcagccagcc
     2641 gagcagcacc gcgaacacga ggatcaggag gatgccgatg aggaaggtgg gcagcgacac
     2701 gccgatgagc gacagcgcga gcatcgcctg cgtcagccag gacttgcgcc gcaacgcggt
     2761 gtacacgccc atccccaccc cggcgagcag cgccagcgag gcggccacga acgacagctc
     2821 gagcgtcgcg ggcagccgct cgacgaacag cttggacacc ggctgcacct ggcgcagcga
     2881 caggccgaac tcgccctgca ccgcgcggcc gacgaatttc gcgtactgga cgtagaacgg
     2941 ctggtccatg ccgagcaggc gcgtgatgcg ctcgcggtcc tccggcgtcg catcctgccc
     3001 gagcatgaac agcaccgggt cgccgacgaa gcggaacagc gagaaggcga tcaggcccac
     3061 ggtcagcatg acgaggaccg cctgtgcgag ccggcgcagg atgaaggcga gcatgggaag
     3121 gggctgggct gcgaggcaat catacaatgc gacatgccgg atttcgcagc ttcgctcatc
     3181 gcctggcagc cccgccacgg ccgccacggc ctgccctggc agggcacgcg cgacccctac
     3241 cgggtctggc tggccgaggt gatgctgcag cagacccagg tcgccgccgc catcccctac
     3301 taccggcgct tcctggcgcg ttttcccgac ctgggtacgc tggccgccgc atcgcaggac
     3361 gaggtgctgc ggctctggag cggcctgggc tactacgcgc gcgggcgcaa tctgcacgcc
     3421 gccgcgcagg cggtggccgc cgccggtggc tttccgacca cggcgcgcgg cctggcggag
     3481 ctgcccgggg tcggccggtc caccgccgcg gcgatcgcgg tgtccgcctt cggccgccgc
     3541 gccgcgatcc tcgacggcaa tgcgaagcgc gtgttcgcgc gctgcttcgg cgtcgaaggc
     3601 gacaaggcgc agtggcgcct ggcggaatcc ctgctgccgc tgcgcgccgt gcgcacctac
     3661 acccaggcgc tgatggacct gggggcgacc gtgtgcacgc gcgcgcagcc cgcctgcggg
     3721 cgctgcccgg tggccgcgcg ctgcgtggcg cgccgcgaag accgcatcca tgagctgccc
     3781 aggcggcgtg cgcgcgcgcc gctgccgctg cgccgcgcca cctggtacgt cctcctgcgc
     3841 gacggaaggg tgctgctcga gcgccgcccg ggcgccggcc tgtggggcgg gctgtggacg
     3901 tttcccgagc ggcgcccgcg cggcctggcg cagctgcgcc ggctgccgcc gctcgatcac
     3961 ggcttcacgc acttccgcct gcgcgcgcag ccgctgctgg gcacggtgcg caaggccgcc
     4021 cccggccagg tgtggctcga tctcgaggat gcggtgggcg ccgcggtgcc cgcgcccgtg
     4081 cgcagcgtgc tgcagtccct acttgccgct tgagctgatc agccggcgca ggatctcgag
     4141 gcgcgcgcgc gcgtcgtcca tctcgagcag gcgctgcttg gccgcgccgg acagcggcag
     4201 caactcggcc aggcgcgcgc tcacccaggc gctcgagtcg tagcgcggcg gcgcttcgac
     4261 caggccggcg ggcgccgcgt cgaggatcct cctgaggacg cgcgcgcaga ggtcggcggc
     4321 atcgagcggt gcatcggttt ccccggcgag gaactcggcc gcggcgcgcg cgaggccgtc
     4381 ggcctgcacg cggcgctcgc ggatgcgaaa gcgcctctcg ccgcgcgcca ccacctgcag
     4441 cacgccgagc tgcggcatgt cccattcgac gatgcgcgcg gtgcacccca cgtcctcggg
     4501 cagggccggc tcgccgactt cgctgccctc gcggatcagg cagacgccga aggccgcgcc
     4561 gtcgcgcagg caggccttgg ccatttccag gtagcgctgc tcgaagatgc gcagcgggag
     4621 gcgcccgccc gggaacagca ctgtctgcag cgggaacagc ggcaggtcgg ccgccgcgct
     4681 cactctttgg cgaggccgcg gtggcgcacc agcacgcgcc agcttccggc gaaggctgcg
     4741 gcgagctgtt ccgccaggta gacggaacgg tgccggccgc cggtgcagcc gatggccacg
     4801 gtgaggtagc tgcggttctc cttcaccacc tctggcagcc agcgcgcgag gaatgcgcgc
     4861 acgtcctcca gcaggcgccg caccggctcc tggccttcga gataagcgat caccgcggcg
     4921 tcgcgcccgg tgagcggccg cagcgccgga tcgtagtgcg ggttgggaag catgcgcgcg
     4981 tcgatcaccc agtcggcgtc cagcgggatg ccgtcgcgga aggcgaagga ctcgaacagc
     5041 agcgcgagcg tgccgccgcc caggccgagc aggtcgcgga tccagttgcg cagcaccttg
     5101 ggctgcagct cgctggtgtc gatgcggtgg ccgagctcgg cgacgccggc cagcaggccg
     5161 cgctcgcgct cgattgcctc ggccagcgtc aggccctcca ccgtcagcgg gtggcgccgg
     5221 cgcgtctccg aaaagcgccg caccagcgtc ggcggattgg cctcgaggaa cagcagccgg
     5281 cagtcggcgc cgcccgcctt gagccgcgcc accgcctcgg gcagcgcggc gagcgccgcg
     5341 ctgcgcgcat ccacgctgag cgccacgcgc tcgtgccccg cgtcgccgag gaagcgcacc
     5401 acgtcctcga gcagcgtcgc cggcaggttg tcgacgcagt agtagccgcc gtcctccagc
     5461 acgtcgagcg cgatgctctt gcccgcgccc gacagcccgc tgaccaggat gagctgcaat
     5521 tgccgcgcta gcccttgagg cagccgctca tgaacttctt gcgctcgtcg cccttcatgc
     5581 cggcggcctg cttgttgcac gcggtcatcc tgtcttgctg cgtgttggcc ttggaagaca
     5641 ggcactcgct catgaacttc ttgcgctcgt cacccttctt gcccgcggct tccttgttgc
     5701 actgggtcat gcgctgctgc tgcgcggtgg gtttcttgtc ctgcgcctgc gccgcgccca
     5761 tcgccaatgt cgcggccgct gccatcacca tcgtcctaag catgttgcct ctcctccggt
     5821 tgcggttgca catcattcgc cggcttccat ggcgcgcttc tggcgcgcca cgaactcggc
     5881 gctggaattg attccccgca gctgcaggat gtagttgcgc accgccgcct cgagcagcac
     5941 ggcgaggttg cgcccggcgg ccaccgggat caccaccttg cgcaccgtca cgccgaggat
     6001 ctcctcggac tgctcggcga gcggcagccg ctcggcgatg tcgcggttgc ccgcgggcgc
     6061 gggcttgtcc aggtgggcga tcaggcgcag gcgcatcttc gggcgcaccg cggtctcgcc
     6121 gaagatggtg cgcacgtcga gcagccccag gccgcgcacc tcgatgaagt ccttcagcac
     6181 cggcggacag cggccctcga gcacgttggc cgccaggcgc gagatctcca ccacgtcgtc
     6241 ggccaccagg ccgtggccgc ggctgatcag ctc
//
LOCUS       DASRIE010000158        23979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_167556, whole genome shotgun sequence.
ACCESSION   DASRIE010000158 DASRIE010000000
VERSION     DASRIE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167556"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFB53_06805"
     CDS             <1..546
                     /locus_tag="VFB53_06805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZN86478.1"
                     /translation="NARTFGHDQRKFLLTTSFIPANLLDSWKAVTEQALSAVERCYAN
                     SSALKNIRLHGDCHAGNILWTDAGPHFVDFDDARSGPALQDLWMLLSGDRAAMSAQLG
                     QVLSGYEQFREFDARELHLLEALRTLRLIHYSAWIARRWDDPAFPAAFPWFGTQRYWQ
                     DRILELREQIALMDEPPLERP"
     gene            complement(543..998)
                     /locus_tag="VFB53_06810"
     CDS             complement(543..998)
                     /locus_tag="VFB53_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZN86479.1"
                     /translation="MAPQFEFLMRIAADVGELLTMGGGPLGERRVVAISGGTFEGPAL
                     KGAIVPGGADWQIARADGVLDIDARYALRTDAGALIRVVSQGYRHGAPEVLAALGRGE
                     EVPAERYFFRTVMRFETGAPGLDWLNRSIAVASAERKARRVLLEAWRLL"
     gene            1021..1875
                     /locus_tag="VFB53_06815"
     CDS             1021..1875
                     /locus_tag="VFB53_06815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86480.1"
                     /translation="MGGHQSEDLEQAAALARAQAEALCRRDPRTGISCSWHHGFWPTL
                     RLLGLVTEPALHGAFLRRALAAIPAARPRVLISGTADHALLAQALAAFGARPFDAVVL
                     DLCETPLMLNRWFAGRAGFAIETRQSNILDFSDDRPYDAVCTHAFLGNFDPAQRAALA
                     AKWRALLRPGGRVVTVNRLRPGSEPLWIAFSTDQVWAFRERVEDAAKARGLRFPRLGR
                     AAEAYAGRQAVYPLGTAEELRALFEQGGFDIEELSLAALDPAARQALGVPAVPGGDSY
                     AQLVAVRR"
     gene            complement(1872..2507)
                     /locus_tag="VFB53_06820"
     CDS             complement(1872..2507)
                     /locus_tag="VFB53_06820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN86481.1"
                     /translation="MAIARICAIRHGETAWNAEGRVQGQTDVPLSAVGEAQARALVPA
                     LAGERFAALYASDLARVLQTAAPAARALGLAPRLDAALRERHYGKFETLTYAEARLRH
                     PEDFARFKAKEPDYDFGSGESLRRFYARSVACLDAIAARHAGEAVLVFTHGGVLEMLR
                     RHAQGTGLSTPRDFEVPNAAINWLEAGPRGWRVLAWADTAHLDAALDDLPG"
     gene            2545..3618
                     /locus_tag="VFB53_06825"
     CDS             2545..3618
                     /locus_tag="VFB53_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN86482.1"
                     /translation="MPESRPHVPRDTVPSIEQEELRGISRTVAEIEWLLLVMVLVYVI
                     FGRPIPDIEPALSMALFFFAAFVMSFRYANFYKRETRWKIAVETLAMIAFISWALWFT
                     GGLASPLLNGYLLVVITAALTLGKTLTLVEVALIGACMLLLDWRLGANDMARLAYWGG
                     FAAQFAPVILVAYITTMFSADIRYGLSRAKLLSETDELTGLYNMRGFVVIASRLFAQA
                     QRYERVASFLMVDSDSLKQVNDAHGHDAGNRLLQHVVKALQGQLRATDVAARYGGDEF
                     VVMLPDTPARGALEVAERIRHAVESAPFAVASAHVPCTVSIGLASFPQDGRTIEALLA
                     CADRALYEAKAGGRNRVAPMRAA"
     gene            complement(3621..4985)
                     /locus_tag="VFB53_06830"
     CDS             complement(3621..4985)
                     /locus_tag="VFB53_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZN86483.1"
                     /translation="MRLTRLILLSLALCLPRAASPAGDSLAAALQEGGAGSGGAVRAE
                     RSPYRGHGTRYDYLPVNLFLGERLYVHADRVGLKLDLDAARRVDLFLAHRYEGTPLDK
                     AAAVLAGMALREQGTDLGIALRIPAAGGTLSAEFLRNVDDASDGAELRLRYGLEWRRG
                     ALRLWPQAVLAWRDAKLNDYYYGVRAAEATADRPAYAAGAGLNLQLALTAAYPLGRDL
                     QLLAGVSATHWAAGVRNSPIVERGLQTAASVGLLFEHSPQPRDWAGTRPLWVKAYHGK
                     ASECDVLQIVLLRCTSTQTPEQTRVTSLEVGRTLIERWNGWHMDLAGYVGLLHHDENG
                     FQPDHWQVNAYFKPYWYGLPWRDRVKTRLGLGTGLSYAGRVPFIEARDQARRGERTNK
                     LITYLDPTIDFSLGDLVGSRALKDTYLGLGVSHRSGIFGLSRLLGNVDGGSNYIYAYL
                     EFAL"
     gene            5084..6175
                     /locus_tag="VFB53_06835"
     CDS             5084..6175
                     /locus_tag="VFB53_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN86484.1"
                     /translation="MPRPRIARAAFVLAACLAAAPACAIEPWKLLAQSRKQMPQPPWP
                     AGDERGMANQIGAATFARCAWHLGQPNAKSYELSQLRSNTMPLTPFAGPYALKAKPSA
                     GIPGSRHGFNSEAYNEGAEPAAQATQFDALGHFAYLRDAWDGKPPYPAEQLRYYGGLA
                     QGEVKPTPDSPLLRLGVEKAPPIVTSAVLLDAQAAVGGGRPLQAGELVTAKHLAAMLK
                     AQGLERRGIQPGDVVYVRTGWGEHWRDPDTEKFYYAKAPGLAYNAAKFLGERRVVAIG
                     LDTPFVDPVPEGMLTGKAGPAAGAPDGLPFAVHHHMLTQMGIHHIENAKLDELARDKV
                     WTSCTIVLPLRDKGAAGSPVRPVAIGAPG"
     gene            6220..7188
                     /locus_tag="VFB53_06840"
     CDS             6220..7188
                     /locus_tag="VFB53_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86485.1"
                     /translation="MHTLLSRCALAALGLALCAAAQAQSFPSRPVRIVVNFPPGGAAD
                     QIARPLAAALQEKLGQPFVVENKPGANGHIGVTEVARQPADGHTLLMSSGGAITVNGQ
                     LNAAISPNPMQALEPVAPAAVVSVYLVVKPAIPANTLAEFIGHARANAGKLNYGSPGT
                     GSSPHLAGEMFNLAAKVKTTHVPYKGAAPVVTDLLGGQLDFTFDPGIGLQHVKAGKLR
                     LLAVGSPRRHPAYPDAPTVAEVVGAEFDADTVFGLYAPAGTPKNVIALLNREISAALQ
                     TDALKARLAAIAAQGLALTPEAFAARNAAEFQRMGALIRETGLAAK"
     gene            7191..8153
                     /locus_tag="VFB53_06845"
     CDS             7191..8153
                     /locus_tag="VFB53_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN86486.1"
                     /translation="MRAWFLQSRGGRSELELREAAAPQPGPGQLVVRVRAAGLNRGEI
                     LGAGAAPKPAGMEAAGEVTQLGAGAAQFKPGDRVMGRCPGAFAEFGLMDERETLPVPG
                     RLSWEEAGAVPVAFLVVYDMLVAQGRLKAGEWLLVAGVTSGVGVYALQAAKALGARVI
                     GTSGSAQKLEQLKALGLDLGLATRAPDFGDAVLKATGGKGADLCINNVGGTVFAECVR
                     SLAFEGRLAVVGYLDGTLSAQIDLDALHARRLTLFGVSNKLRNSEQRSTTVRGFARDF
                     LPLLADGRVRPLLDRSYGFDELPAAVARMEANAHLGKIVLKNGV"
     gene            complement(8163..9125)
                     /locus_tag="VFB53_06850"
     CDS             complement(8163..9125)
                     /locus_tag="VFB53_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86487.1"
                     /translation="MQRLLFGLAALACACGAAAQGGFPGRTISMVVGFAPGGGTDAAA
                     RIIAKAASEGLGQPVVVENRAGAGGNIATEQVAKAPPDGHTILLASVGPLTVAPHMIA
                     NLPYDPLKDLAPVTMAVTFANVLVVHDEVPVRSFVEYVKHAQQQGGNLTYGSSGIGGA
                     GHLSGELFAMMAKVKIVHVPYKGGGPAMQGLLGKQVASIFATPISAGPHIKSGRIRAL
                     AVTSAQRTEFMPEVPTVAESGYPGFEATNWYAYVLPAKTPNEIVARWNRELVKVLNDA
                     GVRKALLDQGMEPAPGSREQLAAYIKREYDTWARVVKEAKITAN"
     gene            complement(9137..10330)
                     /locus_tag="VFB53_06855"
     CDS             complement(9137..10330)
                     /locus_tag="VFB53_06855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN86488.1"
                     /translation="MSAPAGTAALSHLRILDLSRVRAGPSCVRQFADFGADVIKIETP
                     AGLDPNEGMGGARNGSDFQNLQRNKRAITLNLKVAEGLEAFRRLVKTADVVVENYRPD
                     VKFRLGIDYESLAKINPRVILASISGFGQDGPYRDRPGFDQIAQGMSGLMSVTGFPGQ
                     GPVRTGAAIADVSAGLLAALGIMTALLERERSGKGQWVQSSLLQAGLALLDFQAARYL
                     KEGEVAPQVGNDHPTSMPTSAYKTADGYINVAATGDGMWKRVCAALGREDLLARPEFA
                     DHKSRAQNRAALNAALNEALATRRSAEWIELLNAAGVPCGPIYAMDQVFADPQVRHLG
                     VAAEVAHKKLGRFRLLNQAVRLSRTPAAIRTAAPEIGEHTDEILAELGYGSDEVARLR
                     AAKAI"
     gene            complement(10394..10804)
                     /locus_tag="VFB53_06860"
     CDS             complement(10394..10804)
                     /locus_tag="VFB53_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86489.1"
                     /translation="MREIQGVGEEAQLQRRWFHDEYFDLFVWQAPGGAVARFDLCYGA
                     GASERALVWLGEGRFFQDGAPESTSDPRELLRRFAQAGAALPQRVRREVTARIQECAG
                     LGPPLPGRRARFRRAPWQQRETSRQAGKGAARQA"
     gene            complement(10869..12164)
                     /locus_tag="VFB53_06865"
     CDS             complement(10869..12164)
                     /locus_tag="VFB53_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN86490.1"
                     /translation="MPEAMDLGLSLQVGKYDVQKMLGKGATGTVYLAKDTFSGHEVAL
                     KTIEPEVFRDPEFGTVYRSQFLNEASLAGKLKHPHIVTILDAVVGEDSGYIAMELVTG
                     GDLAAHTKPDKLLPVADVLQIGFKCCGALDYAFHEGIVHRDIKPANLMIGKGTDVKIA
                     DFGAAYLRKSQVVQTAMMGSPYYMSPEQIAGRTLTHHSDMYSLGAVLYELLTGQLPAR
                     AENLEALLKKITQLDPLPPSEIRKDLPRELDAVILRAMRKDPAQRYPTWAEFALELSK
                     VVRQVLPANAVPDTEKYVALKNVEMLAKLADAEIWELVNAGKWTRVDKGRIVVRENEK
                     GSSFFFLAEGEVKVTRAGRLLNVVNHGECFGEMAYIWGGALPRHATVESMTKLMLAEF
                     EPAALGRMSGGAQLQLTRALVRNLVDRLVLSNTRFTAAR"
     gene            12281..13510
                     /locus_tag="VFB53_06870"
     CDS             12281..13510
                     /locus_tag="VFB53_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HZN86491.1"
                     /translation="MAHKEVPVEYLEFGMFVARLDRPWTETPFVYQGFVLKTEQQLLA
                     LKKYCKKVYVDPEKMERVEPPKLTLPPNARAAIAAQAKEVVRGTEVYKELASVEEELP
                     RARSVFQQTGAVVEALSRSVLEGNALDGNRARQAVVEITDSVVRNPDAMMLLVTMREK
                     SAQTLNRATQVSVYMTVFGRFLQLARDRLELLGLMGLLQDVGMLKLPAGLPERRSNLT
                     GAEEESFKSHIGHSVEILSQTPGLPAELPGLASLHHERYDGSGYARGLKGAAISQMGA
                     IAGIVDTFDVLTAPAPYGMQLPPSNALNVLFQNRGTQFHAALIEQFIQCVGAFPVGSV
                     VELNSGEVGVVITQNLVKRLQPRVMVVQDADGNPMHPHKILDLVNEPMANEDEPYRIR
                     RTLDQTTVSINSRELFL"
     gene            13524..15269
                     /locus_tag="VFB53_06875"
     CDS             13524..15269
                     /locus_tag="VFB53_06875"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HZN86492.1"
                     /translation="MDPAAIQHLMDEFGVDAAAIAALDALGAALRAQAPEALLAARGE
                     SALSAATSPFFLPLLPTGMSEPQRALVNALSLGALARESLAQLLQEWAATLNPEEPTE
                     RAQRDCRPLQDLLRAGALRMYAGEPARCEAALAAAERLGALQSAIMAEVAARGAHARP
                     AAVAGLGAFLQRLEACVAARAADGQQLALLFVDCGIIGRIDGVWGYTVGDGVRDRLSA
                     RLGSEVLRAQDLLGDPGRDEFACVLASVQSAGVSVLAAEKILRTLDAPLWVDVEEVYA
                     RPAVGIALYPEDGASGVELLQRARVAARAARERPERFAAYSAEQEGLEAELLVYENRL
                     RTAIAQDALDIVFQPQRDQRNGQIVGAESQLRWRDASLGAVPVQRAIAAAESAGLVNE
                     VTLWLVNGALRNCREFRDAAGLDLRVGVNLSAKSLRQADLPDFIASALKTWNLRPSRL
                     ALGITETAVLARSLESVETLQRLREAGVRLCLDDPGAGYGSLAWLSTLPFHELRVDLA
                     AVGDVAESPQHLALVRTLVDFAHRLKMEVLVSGVDDEATLAKVQPLGCDYLQGALVGA
                     PLDAAAFAGRHRADQ"
     gene            15283..16245
                     /locus_tag="VFB53_06880"
     CDS             15283..16245
                     /locus_tag="VFB53_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN86493.1"
                     /translation="MRNRLICTLAALALPLAALAQSGYPRKPILMLVPLQAASAVDNA
                     ARVVAQKMSENMGQAIVIENQPGAAGLIGAERVAKAAPDGYVIGGFNDSIMTMLPNVH
                     ARMPWDILRDFEPVSLVATMEWALVAPPGSPYRSAADLIAAARARPGALNYSSGGNGS
                     PQHVAMELFKSRAGVFMTHIPYRGAAAAAVDTAAGQVDASFSGMPTVFGMIKGGKLRL
                     LGVASPARSALFPEAPAVGESLPGFVFNSNFSVVAPAGTPKEIVARLNAEIAKATSAP
                     EVRDKLVAQGFTVNATSPQELARFTREQLARYAALFKQAGIRAD"
     gene            complement(16242..16751)
                     /locus_tag="VFB53_06885"
     CDS             complement(16242..16751)
                     /locus_tag="VFB53_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZN86494.1"
                     /translation="MTPISMYQASAPRFANTLNNLSAILDKAQAHCEAKKIDPLALTS
                     FRLYPDMLPFTRQITIACDTAKGAVARLAGVENPKHEDSEKTFEELKARIAKTVAFIG
                     GVKAAQLDGSEDKVIVLKLGPREVTFQGLQYLLGFAQPNFYFHAVTAYDILRHNGVEL
                     AKRDYIGNP"
     gene            16837..17667
                     /locus_tag="VFB53_06890"
     CDS             16837..17667
                     /locus_tag="VFB53_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN86495.1"
                     /translation="MAAPNALYDPAVALAFFRSAGKAERYAPGATIFAENAKARPLLL
                     QQDKMYYVYDGEVSLMAKNKVIGRVLEGEVFGELAPLTRAPRSAAAVAKTACRVISLD
                     DEEFRTALMKRPAFALMLMSVMVTRLRQTIAALRDAGVLSVSGAWKEAAVFDKRQLAD
                     LVQGLANDPQIYYERGAMVIKEGAMGARMYTVLEGRVAVTIAGSMVERVGPGGVFGEA
                     ALVDQATRMASVQAETDCSLQPVGRAAFLELVKVSPVFAERMLAALAQRLRLLTARLD
                     "
     gene            complement(17648..18211)
                     /locus_tag="VFB53_06895"
     CDS             complement(17648..18211)
                     /locus_tag="VFB53_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86496.1"
                     /translation="MGLLRGVWGHSNCAVKGAGESVNRRRAAGVVRRMVRLRCPEEPG
                     VALHLVQRGGAADPAAYLERLRAAAAERGCPVHAYVLMGSHAHFLLTPARRAAATAML
                     LELGGEVDATPVIAPRYLLACMRYIELNPVRAGIVRRPAQYPWSSYRANALGEADALV
                     TPHPWYYALGRTPQARRAAYRASPGAR"
     gene            18210..19160
                     /locus_tag="VFB53_06900"
     CDS             18210..19160
                     /locus_tag="VFB53_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN86497.1"
                     /translation="MRQLVAALLFVAAAAAQAQYPQRPVQVIVPWGAGGGTDATARII
                     ATLLEKELKQPFNVVNRTGGSGVVGHDAIAKSAPDGYTIGMITVEITMMHHVGLTPLK
                     HTDYTPIGLVNADPAGVHVRVDSPYKSVKDLLAAIKASPGKMKASGTGQGGIWHLAIA
                     GLLKDQGIDPNALPWVPSNGAAPGLQDMVAGGVEVVPCSVPEARGMIDAGKVRALAIM
                     DANPPALYPNVPTLKKELGTDWAIAAWRVIAAPRGIPAEAQKTLTTALKKVYDSKEYK
                     DFMASRGFGVIWADPAATAKFMAASDANLGAALKAVGLAK"
     gene            19162..19623
                     /locus_tag="VFB53_06905"
     CDS             19162..19623
                     /locus_tag="VFB53_06905"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN86498.1"
                     /translation="MKVNDALSGAGLAALGGVVLWHIQGFPAMPGQKFGPAWFPGAIA
                     IGLLACGLLLMLRAERGPWIALPPWTRRARPLAGVAAVVGGLLAYVLAADRLGFHLTG
                     FALLALWCRILGAGWRASVAVSAIATLAIHLSFYKLLRIPLPWGVLERFAF"
     gene            19625..21121
                     /locus_tag="VFB53_06910"
     CDS             19625..21121
                     /locus_tag="VFB53_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN86499.1"
                     /translation="MSAEILAQALAQVFDPYVLWVILGSAVFGLFVGAVPGLTATMAT
                     ALLVPVTFFMPPVPAIASIVTATAMAIFAGDIPSCFLRMPGTPASAAYTDEAYAMTRK
                     GQGELALGASLVFSVIGGLFGTAVLILAAPALAEVALRFSSFEYFWLVVLGLASAVFL
                     ALANPLKGIVSLLLGLLVASVGLDNPAGFPRFTFGNAELAGGLTLIPIMIGMFAVSEV
                     IRNVAAIERPWEMAQATIGNVFQGMWGLLRRYPLQTLRGSVLGTAIGALPGAGADIAA
                     WMSYAVSKKFSRERGKFGSGHVEGIIEAGASNNSALAGAWIPALVFGIPGDSITAIVI
                     GVLYIKGLNPGPTIFMTNAVAIYAIFMVFILANLIMLPLGVAAIKGAKQMLRAPRELL
                     LPVILLFCIVGSFAINNSAFGVLLMLAFGVAAYLMEENGFPVAPAILGMVLGAMLEEN
                     FISSMIKADGRLLAFFERPIAGGLGALAIAVLLVPVLRARLRRGAPAR"
     gene            21154..21435
                     /locus_tag="VFB53_06915"
     CDS             21154..21435
                     /locus_tag="VFB53_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86500.1"
                     /translation="MSAPQKPAAAAPHDPIQEMAEKLYVALCARVYSAAGEKPQPKAV
                     AQLCFKLAETFQAANYEFNPTAVAARAAKDKASVSLDSVNIDFGAVGKS"
     gene            21438..22868
                     /locus_tag="VFB53_06920"
     CDS             21438..22868
                     /locus_tag="VFB53_06920"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN86501.1"
                     /translation="MRVGIIGAGFAGLGAAIRLRQAGFTDVTVYERAAELGGTWRDNT
                     YPGCACDIPSVLYSFSFAPNPEWTRMYPSQPEIWDYLRRLARAHGLLERIRFGAEMRA
                     ARWEEASRTWRLSFKDGSEASVDVLVSASGFLNKPALPQLPGLGGFEGAAFHSSQWRH
                     DVDLAGKRVALVGTGASAIQILPEIAKTAAKVHVFQRTPPWVLPRLDRAFSARERRWF
                     RRLPLLQRLSRWRLYLRQELLVTGFLGGAWIQKRFRQYAAGLLERQVPDAALRAKLTP
                     AYAPGCKRLLVSNDWYPALQRPNVELLAEPAARLEGRRIVGASGAAREVDAVVFATGF
                     IATEFVAPVKVHGRGGVELGELWKDGAASHLGISVAGFPNAFFIVGPNTGLGHNSIIF
                     MIEAQLNYLVGALKSGAQVLELLPEVQSKSYAAVQRRMKKTVWASGCRSWYLSADGRN
                     DTLWPGSTLEYWWRTLRFDAASYRSG"
     gene            complement(22860..23921)
                     /gene="leuB"
                     /locus_tag="VFB53_06925"
     CDS             complement(22860..23921)
                     /gene="leuB"
                     /locus_tag="VFB53_06925"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZN86502.1"
                     /translation="MNIAVMPGDGIGKEVTAEAVKVLQALAGSDAKFTEAPIGAAGVA
                     AAGVPLPPATLELARKADAILFGAVGTPGDESGPRDKRPGLGLLTLRRELKLFANFRP
                     AFLFPELVGASSLKPELVQGLDLIILRELNGDIYYGEPRGIRVGDSGRREGVNTMRYT
                     EPEIERIAHVAFRTARERRRKVCSVDKANVLETMQLWRETVARIGRQYPNVALTHLFV
                     DAASMQLLRAPKQFDVMVMGNMFGDILSDAAAMLTGSIGMLPSGSIGENRFGLYEPVH
                     GSAPDIAGKDLANPLAAILSAAMMLRMSFGDRDAAERIERAVRKALAQGLRTADMMQP
                     GKRQVGCAAMGDAVAAALA"
BASE COUNT         3298 a         8721 c         8484 g         3476 t
ORIGIN      
        1 aacgcccgga ccttcggcca cgatcagcgc aagttcctcc tcaccaccag cttcatcccc
       61 gccaacctgc tcgattcatg gaaggcggta accgagcagg cgttgtccgc agtcgaacgt
      121 tgttatgcaa attcttctgc gctgaaaaac atccggctgc acggcgactg ccacgccggc
      181 aacatcctgt ggaccgacgc ggggccccac ttcgtcgact tcgacgacgc gcgcagcggc
      241 ccggccctgc aggacctgtg gatgctgctc tcgggggacc gggcggcgat gagcgcgcaa
      301 ctcggccagg tgctcagcgg ctacgagcag ttccgcgagt tcgatgcgcg cgagctgcac
      361 ctgctggaag ccctgcgcac cctgcgcctg atccactact cggcctggat cgcgcggcgc
      421 tgggacgatc cggccttccc cgccgccttc ccctggttcg gcacgcagcg ctactggcag
      481 gaccggatac tcgagctgcg cgagcagatc gcgctgatgg acgagccgcc gctcgagcgg
      541 ccctaaagca agcgccacgc ctccagcagc acccggcgcg ccttgcgctc ggcgctggcg
      601 acggcgatgc tgcggttgag ccagtcgagc cccggtgcgc ccgtctcgaa gcgcatcacg
      661 gtgcggaaga aataccgctc cgccggcacc tcctcgccgc gaccgagcgc cgccagcacc
      721 tcgggcgcgc cgtggcggta gccctggctc accacccgga tgagggcgcc ggcgtcggtg
      781 cgcagcgcat agcgcgcgtc gatgtcgagc acgccgtcgg cgcgcgcgat ctgccagtcc
      841 gcgccgcccg ggacgatggc gcccttgagc gcggggccct cgaatgtccc gccgctgatc
      901 gccaccacgc gccgctcgcc cagcgggccg ccgcccatgg tcagcaactc gccgacgtcg
      961 gcggcgatgc gcatcaggaa ttcgaattgc ggggccattg ccggtatcct gccatgctgg
     1021 atgggggggc atcaaagcga ggacctggag caggcggcgg cgctcgcgcg cgcgcaggcg
     1081 gaggcgcttt gccggcgtga cccgcgcacc ggcatctcct gcagctggca ccacggcttc
     1141 tggccgacgc tgcgcctgct cgggctggtg accgagcccg cgctgcacgg cgcgttcctg
     1201 cgccgcgcgc tcgccgcgat cccggccgcg cgcccgcgcg tcctgatctc gggcaccgcc
     1261 gaccacgcgc tcctcgcgca ggcgctcgcc gccttcggcg cgcggccttt cgacgccgtg
     1321 gtgctcgacc tgtgcgagac gccgctgatg ctcaaccgct ggttcgccgg gcgcgccggc
     1381 ttcgccatcg agacgcggca gtcgaacatc ctcgattttt ccgacgaccg gccctacgac
     1441 gcggtctgca cgcacgcctt cctcggcaac ttcgacccgg cgcagcgcgc cgcgctggcg
     1501 gccaagtggc gcgcgctgct gcgcccgggc ggcagggtgg tcacggtcaa tcgcctgcgg
     1561 ccgggcagcg agccgctgtg gatcgcgttc agcaccgacc aggtgtgggc gttccgcgag
     1621 cgcgtggagg acgccgccaa ggcgcgcggc ctgcgctttc cccgcctggg ccgcgccgcc
     1681 gaggcctatg ccggccggca ggccgtctac ccgctgggca ccgcggagga actgcgcgcg
     1741 ctgttcgagc agggcggctt cgacatcgag gagctctcgc ttgccgccct cgacccggcg
     1801 gcgcgccagg cgctcggcgt gcccgccgtg ccgggcggcg acagctacgc gcaactcgtc
     1861 gcggtgcggc gctagcccgg caggtcgtcc agcgccgcgt cgaggtgcgc ggtgtcggcc
     1921 caggcgagca cgcgccagcc gcgcggcccc gcctcgagcc agttgatggc ggcgttgggc
     1981 acctcgaagt cgcgcggcgt cgacaggccg gtgccctgcg cgtgccggcg cagcatctcc
     2041 agcacgccgc cgtgcgtgaa caccagcacc gcctcgccgg cgtggcgcgc ggcgatggcg
     2101 tccaggcagg ccacgctgcg cgcgtagaag cgccgcaggc tctcgccgct gccgaagtcg
     2161 tagtcgggct ccttcgcctt gaagcgcgcg aagtcctccg ggtggcgcag gcgcgcctcg
     2221 gcgtaggtca gcgtctcgaa cttgccgtag tgccgctcgc gcagcgcggc gtccaggcgc
     2281 ggcgccaggc cgagcgcgcg cgccgccggc gccgcggtct gcagcacgcg cgccagatcg
     2341 ctggcgtaca gcgcggcgaa gcgctccccg gcgagcgccg gcaccagcgc gcgtgcctgg
     2401 gcctcgccca ccgccgacag cggcacgtcg gtctgtccct gcacgcgccc ttccgcgttc
     2461 caggcggtct cgccgtgacg gatggcgcag atcctcgcaa ttgccatttt tgcatcccgc
     2521 tccagccggt atcatagcgg cgccgtgccc gaatcccgcc cccacgtgcc gcgcgacacc
     2581 gtgcccagca tcgagcagga ggaactgcgc ggcatctcgc gcacggtcgc cgagatcgaa
     2641 tggctgctgc tggtgatggt gctggtgtac gtgatcttcg gccggccgat ccccgacatc
     2701 gagccggcgc tgtcgatggc cctgttcttc ttcgcggcct tcgtcatgag cttccgctac
     2761 gccaacttct acaagcgcga gacgcgctgg aagatcgcgg tggagaccct ggcgatgatc
     2821 gcgttcatca gctgggcgct gtggttcacc ggcggcctcg ccagcccgct gctcaacggc
     2881 tacctgctgg tggtgatcac cgccgcgctc accctgggca agaccctgac cctggtcgag
     2941 gtggcgctga tcggcgcctg catgctgctg ctcgactggc ggctgggcgc caacgacatg
     3001 gcgcgcctcg cctactgggg cggcttcgcc gcgcagttcg cgccggtgat cctggtggcc
     3061 tacatcacca ccatgttctc ggccgacatc cgctacggcc tgtcgcgcgc caagctgctg
     3121 tcggagaccg acgagctcac cggcctgtac aacatgcgcg gcttcgtggt gatcgccagc
     3181 cgcctgttcg cgcaggcgca gcgctacgag cgcgtggcca gcttcctgat ggtggattcc
     3241 gacagcctga agcaggtgaa cgacgcgcac ggccacgacg ccggcaaccg gctgctgcag
     3301 cacgtggtga aggcgctgca ggggcagctg cgcgccaccg acgtggcggc gcgctacggc
     3361 ggcgacgagt tcgtggtgat gctgccggac accccggcgc gcggcgcgct ggaagtcgcc
     3421 gagcgcatcc gccacgcggt cgagtccgcc cccttcgccg tcgcgtcggc gcacgtgccc
     3481 tgcacggtga gcatcggcct ggccagcttc ccgcaggacg ggcgcaccat cgaggcgctg
     3541 ctcgcctgcg ccgaccgcgc gctgtacgag gcgaaggcag gcgggcgcaa ccgcgtggcg
     3601 ccgatgcgcg ccgcctgacg ctacagcgcg aactcgagat aggcgtagat gtagttcgag
     3661 ccgccgtcga cgttgcccag cagccgtgac aggccgaaga tgcccgagcg gtgcgatacg
     3721 ccgaggccga ggtaggtgtc cttcagcgcg cggctgccga cgaggtcgcc gaggctgaag
     3781 tcgatggtcg gatccaggta ggtgatgagc ttgttggtgc gctcgccgcg ccgcgcctgg
     3841 tcgcgcgcct cgatgaatgg cacgcggccg gcgtaggaca gccccgtgcc caggccgagg
     3901 cgcgtcttca cgcggtcgcg ccagggcagg ccgtaccagt agggcttgaa gtaggcgttg
     3961 acctgccagt gatcgggctg gaagccgttc tcgtcgtggt gcagcaggcc gacgtagccg
     4021 gcgaggtcca tgtgccagcc gttccagcgc tcgatcagcg tgcgcccgac ctccagcgag
     4081 gtgacgcgcg tctgctccgg cgtctgcgtg gaggtgcagc gcagcagcac gatctgcagc
     4141 acgtcgcact cgctcgcctt gccatggtag gccttcaccc acagcgggcg cgtcccggcc
     4201 cagtcgcgcg gctgcggcga gtgctcgaac agcaggccga cgctggccgc ggtctgcaag
     4261 ccgcgctcga cgatcgggct gttgcgcacg ccggccgccc agtgcgtcgc cgacacgccg
     4321 gcgagcagct gcaggtcgcg tcccagggga taggccgcgg tcagcgcgag ctgcaggttg
     4381 aggcccgcgc cggccgcgta ggcggggcgg tccgcggtgg cttccgcggc gcgcaccccg
     4441 tagtaatagt cgttcagctt cgcgtcgcgc caggcgagca cggcctgcgg ccacaggcgc
     4501 agcgcgccgc ggcgccattc gaggccgtag cgcaggcgca gctccgcgcc gtccgaggca
     4561 tcgtccacgt tgcgcaggaa ctcggcgctc agcgtgccgc cggcggccgg gatgcgcaag
     4621 gcgatgccga ggtcggtgcc ctgctcgcgc agcgccatgc ccgcgagcac cgccgccgcc
     4681 ttgtccagcg gcgtgccctc gtagcggtgc gcgagaaaca gatccacgcg gcgcgcggcg
     4741 tccaggtcca gcttcaggcc gacgcggtcc gcgtgcacgt acagccgctc gccgaggaac
     4801 aggttgaccg gcaggtagtc gtagcgcgtg ccgtgcccgc ggtacggcga gcgctcggcg
     4861 cgcaccgccc cgcccgatcc cgcgccgccc tcctgcagcg cagcggcgag gctgtcgccg
     4921 gcgggcgacg cggcgcgcgg caggcagagc gcgagcgaca acaggataag gcgcgtcagc
     4981 cgcacgcggc gcaatttcga ctctctcccc ttgcagaccg cgcgagctta cagggaatca
     5041 cgctagcatt gcggcgacga accgttttcc tcccggagtc cgcatgcctc gtcctcgcat
     5101 cgcccgcgcc gccttcgtcc tcgccgcctg cctcgccgcc gcgcccgcct gcgccatcga
     5161 gccgtggaag ctgctggcgc agtcgagaaa gcagatgccg cagccgccct ggcccgcggg
     5221 cgacgagcgc ggcatggcca accagatcgg cgccgcgacc ttcgcgcgct gcgcctggca
     5281 cctcggccag ccgaacgcga agtcctacga gctgtcgcag ctgcgctcca acaccatgcc
     5341 gctcacgccg ttcgccgggc cctacgcgct caaggcgaag ccgagcgccg gcattcccgg
     5401 cagccgccac ggcttcaaca gcgaggccta caacgaaggc gccgagcccg ccgcgcaggc
     5461 gacgcagttc gacgcgctcg ggcatttcgc ctacctgcgc gatgcctggg acggcaagcc
     5521 gccgtatccc gcggagcagc tgcgctacta cggcggcctg gcgcaaggcg aggtcaagcc
     5581 cacgcccgac tcgccgctgc tcaggctcgg cgtggaaaag gcgccgccca tcgtcaccag
     5641 cgccgtgctg ctcgacgccc aggccgcggt cggcggcggc cggccgctgc aagcgggcga
     5701 gctggtcacc gccaagcacc tcgcggcgat gctgaaggcg cagggcctgg agcggcgcgg
     5761 catccagccc ggcgacgtgg tctacgtgcg caccggctgg ggcgaacact ggcgcgaccc
     5821 ggacaccgag aagttctact acgcgaaggc gccgggcctg gcctacaacg cggcgaaatt
     5881 tctcggcgag cggcgcgtgg tggcgatcgg cctcgatacg ccgttcgtgg acccggtgcc
     5941 cgagggcatg ctgacgggca aggccgggcc cgcggcgggc gcgcccgacg ggctgccgtt
     6001 cgcggtgcac caccacatgc tcacgcagat gggcatccac cacatcgaga acgcgaagct
     6061 ggacgagctc gcgcgcgaca aggtgtggac ctcgtgcacc atcgtcctgc cgttgcgcga
     6121 caagggcgcc gcgggctcgc cggtgcggcc ggtggccata ggagcgcctg gctgacgggc
     6181 gcaatgctag gattgccccg cccacaggag ggggccccga tgcacacact gctgtcccgc
     6241 tgcgcgctcg ccgcgctcgg cctcgcgctc tgcgccgcgg cgcaagcgca gtccttcccg
     6301 tccaggccgg tgcgcatcgt cgtcaacttc ccgcccggcg gcgccgccga ccagatcgcg
     6361 cggccgctcg ccgccgcgct gcaggaaaag ctcggccagc ccttcgtggt ggagaacaag
     6421 cccggcgcca acggccacat cggcgtcacc gaagtggcca ggcagcccgc cgacgggcac
     6481 accctgctca tgtcctcggg cggcgccatc accgtcaacg gccagctgaa cgcggccatc
     6541 tcgcccaacc cgatgcaggc gctcgagccg gtggcgccgg cggcggtggt gagcgtgtac
     6601 ctggtggtga agccggcgat ccctgccaat acgctggccg aattcatcgg ccacgcgcgc
     6661 gccaacgccg gcaagctcaa ctacggctcg cccggcaccg gcagctcgcc gcacctcgcc
     6721 ggcgagatgt tcaacctggc ggcgaaggtg aaaaccacgc acgtgcccta caagggcgcg
     6781 gcgccggtgg tcaccgacct cctcgggggc cagctcgact tcaccttcga cccgggcatc
     6841 ggcctgcagc atgtgaaggc cggcaagctg cgcctgctgg cggtgggcag ccccaggcgc
     6901 caccccgcct accccgacgc gccgacggtc gccgaggtgg tgggcgccga gttcgacgcc
     6961 gacacggtgt tcggcctcta cgctcccgcc ggcacgccga agaacgtgat cgcgctgctc
     7021 aaccgcgaga tcagcgccgc gctgcagacg gatgccctga aagcgcgcct cgcggcgatc
     7081 gcggcgcagg gcctcgccct cacgccggaa gccttcgcgg cgcgcaacgc cgccgagttc
     7141 cagcgcatgg gcgcgctgat ccgcgaaacg ggcctggcgg cgaagtagcc atgcgcgctt
     7201 ggttcctcca gtcgcgcggc ggccgcagcg agctcgagct gcgcgaagcg gcggcgccgc
     7261 agcccggacc cggccagctc gtggtgcgcg tgcgcgccgc cgggctgaac cgcggcgaga
     7321 tcctcggcgc cggcgccgcg ccgaagccgg ccggcatgga ggccgcgggc gaggtgacgc
     7381 agctcggcgc cggcgccgcg cagttcaagc ccggcgaccg cgtgatgggc cgctgccccg
     7441 gcgccttcgc cgagttcggc ctgatggacg agcgcgagac cctgccggtg cccgggcgcc
     7501 tgtcctggga ggaagccggc gcggtgccgg tggccttcct ggtggtgtac gacatgctgg
     7561 tcgcccaggg ccggctgaag gcgggcgagt ggctgctggt cgccggcgtc acctcgggcg
     7621 tgggggtgta cgcgctgcag gcggccaagg ccctcggcgc cagggtgatc ggcacctcgg
     7681 gctcggcgca gaagctcgaa cagttgaagg cgctgggact cgacctgggg ctcgccacgc
     7741 gcgcgccgga tttcggcgac gcggtgctca aggccaccgg cggcaagggc gccgacctgt
     7801 gcatcaacaa tgtcggcggc acggtgttcg ccgaatgcgt gcgttcgctc gccttcgagg
     7861 gcaggctggc cgtcgtcggc tacctggacg gcacgctcag cgcgcagatc gacctcgacg
     7921 cgctgcacgc caggcgcctc acgctgttcg gcgtgtccaa caagctgcgc aattccgagc
     7981 agcgcagcac cacggtgcgc ggcttcgcgc gcgatttcct gcccctgctc gccgacggcc
     8041 gcgtgcggcc gctgctcgac cgcagctacg gcttcgacga gctgccggcg gccgtggcgc
     8101 gcatggaagc caacgcgcac ctgggaaaaa tcgttctaaa aaatggggtc tgaccccatt
     8161 tttcagttgg cggtgatttt cgcttccttc accacccgcg cccaggtgtc gtactcgcgc
     8221 ttgatgtagg cggcgagctg ctcgcggctg cccggcgccg gctccatgcc ctggtcgagc
     8281 agcgccttgc gcacgccggc gtcgttgagc accttgacca gctcgcggtt ccagcgcgcg
     8341 acgatctcgt tgggggtctt cgccggcagc acgtaggcgt accagttggt ggcctcgaag
     8401 cccgggtagc ccgactcggc gacggtcggc acctcgggca tgaactcggt gcgctgcgcg
     8461 ctggtcacgg cgagcgcgcg gatgcgtccg gacttgatgt gcggcccggc cgagatcggc
     8521 gtggcgaaga tcgaggcgac ctgcttgccg agcaggccct gcatcgccgg cccgcccccc
     8581 ttgtagggca cgtgcacgat cttcaccttc gccatcatgg cgaacagctc gcccgacagg
     8641 tggcccgcgc cgccgatgcc cgaggagccg taggtgaggt tgccgccctg ctgctgcgcg
     8701 tgcttcacgt actcgacgaa gctgcgcacc ggcacttcgt cgtgcaccac caggacgttg
     8761 gcgaaggtca ccgccatggt gaccggcgcc aggtccttca gcgggtcgta aggcaggttg
     8821 gcgatcatgt gcggcgccac ggtgagcgga cccaccgagg cgagcaggat ggtgtggccg
     8881 tcgggcggcg ccttcgccac ctgctcggtg gcgatgttgc cgccggcgcc ggcgcgattc
     8941 tccaccacca ccggctggcc gaggccttcg ctcgccgcct tggcgatgat gcgcgccgcc
     9001 gcgtcggtgc cgccgcccgg cgcgaagccg accaccatcg agatggtgcg gccgggaaaa
     9061 cctccttgcg ccgcggcgcc gcaggcgcag gccagcgcgg ccaatccgaa cagcagtcgt
     9121 tgcatggcgc tcctccctag attgccttcg ccgcgcgcag ccgcgcgacc tcgtcgctgc
     9181 cgtagcccag ctcggcgagg atctcgtcgg tgtgctcgcc gatctccggc gccgccgtgc
     9241 ggatggcggc cggcgtgcgc gacagcctca ccgcctggtt gagcaggcgg aaacggccga
     9301 gcttcttgtg cgccacctcg gccgcgacgc cgaggtgccg cacctgcggg tcggcgaaca
     9361 cctggtccat ggcgtagatc gggccgcagg gcacgcccgc ggcgttgagc agctcgatcc
     9421 actcggcgct gcgcctcgtg gcgagcgcct cgttgagcgc cgcgttcagc gccgcgcggt
     9481 tctgcgcccg cgacttgtgg tcggcgaact ccggccgggc gagcaggtcc tcgcgcccga
     9541 gggccgcgca cacgcgcttc cacatgccgt cgccggtggc ggcgacgttg atgtagccgt
     9601 cggcggtctt gtaggccgag gtcggcatgc tggtggggtg gtcgttgccc acctgcggcg
     9661 cgacttcgcc ctccttcagg tagcgcgcgg cctggaagtc gagcagcgcc agccccgcct
     9721 gcagcagcga ggattgcacc cactgcccct tgcccgagcg ctcgcgctcc agcagcgcgg
     9781 tcatgatgcc cagcgccgcc agcagccccg ccgacacgtc ggcgatcgcc gcgccggtgc
     9841 gcaccggccc ctggccggga aagcccgtca ccgacatcag gccgctcatg ccctgcgcga
     9901 tctggtcgaa acccggccgg tcgcggtacg ggccgtcctg gccgaagccc gagatgctgg
     9961 cgaggatcac gcgcggattg attttcgcca gcgactcgta gtcgatgccg aggcggaact
    10021 tcacgtccgg ccggtagttc tccaccacca cgtcggcggt cttcaccagg cgccggaacg
    10081 cctccagccc ctcggccacc ttgaggttga gcgtgatggc gcgcttgttg cgctgcaggt
    10141 tctggaaatc cgagccgttg cgcgccccgc ccatgccctc gttgggatcc aggccggccg
    10201 gggtctcgat cttgatcacg tcggcgccga agtcggcgaa ctggcgcacg cagctcgggc
    10261 cggcgcgcac gcgcgacagg tcgaggatgc gcagatgcga gagcgcggcg gtgccggcgg
    10321 gtgcggacat ggcgcgcatg ttagcccgag ccgcgcccgc cgcgccatgc cgggcaggcc
    10381 gggttcgccg gcgctaggcc tggcgcgccg cgcccttgcc cgcctgccgg ctcgtctcgc
    10441 gctgctgcca gggcgcgcgc cggaagcgcg cgcgccgccc gggcagcggg ggtccgagcc
    10501 cggcgcattc ctggatgcgc gcggtcacct cgcgccgcac gcgttgcggc agcgcggcac
    10561 cggcctgcgc gaagcggcgc agcagttcgc gcggatcgga ggtggactcg ggcgcgccgt
    10621 cctggaagaa acgcccctcg cccagccaga ccagggcgcg ctcgctggcg cccgcgccgt
    10681 agcacaggtc gaagcgcgcc accgccccgc ccggcgcctg ccagacgaaa aggtcgaagt
    10741 actcgtcgtg gaaccagcgc cgctgcagct gcgcctcttc gcccacgccc tggatctcgc
    10801 gcatcacccg cgttagacgc ccgcgcgcgc ccgcagttcg cggcacgcgc tgtcgccgcc
    10861 gggcgacgct agcgcgcggc ggtgaaccgg gtgttggaga gcaccaggcg gtcgaccagg
    10921 ttcctcacca gcgcgcgcgt gagctgcagc tgggcgccgc cgctcatcct gccgagggcc
    10981 gccggctcga actcggcgag catcagcttg gtcatcgact cgacggtggc gtgccgcggc
    11041 agcgccccgc cccagatgta ggccatctcg ccgaagcact cgccgtggtt caccacgttg
    11101 agcagccggc cggcgcgcgt caccttgacc tccccctcgg cgaggaagaa gaagctgctg
    11161 cccttctcgt tctcgcgcac cacgatcctg cccttgtcca cgcgcgtcca cttgccggcg
    11221 ttcaccagct cccagatctc ggcgtcggcg agcttggcga gcatctccac gttcttcagc
    11281 gccacgtact tctcggtgtc cggcacggcg ttcgccggca gcacctggcg caccaccttg
    11341 gacagctcga gggcgaactc cgcccaggtc gggtagcgct gggccggatc cttgcgcatg
    11401 gcgcgcagga tcaccgcgtc gagctccctg ggcaggtcct tgcggatctc gctcggcggc
    11461 agcgggtcga gctgggtgat cttcttcagc agcgcttcca ggttctcggc gcgcgccggg
    11521 agctgcccgg tgagcagctc gtagagcacc gcgccgagcg agtacatgtc gctgtggtgg
    11581 gtgagcgtgc gcccggcgat ctgctcgggc gacatgtagt agggcgagcc catcatcgcg
    11641 gtctgcacca cctggctctt cctcaggtag gcggcgccga aatcggcgat cttcacgtcg
    11701 gtgcccttgc cgatcatcaa attggcgggc ttgatgtcgc ggtgcacgat gccttcgtga
    11761 aacgcgtagt cgagcgcgcc gcagcacttg aagccgatct gcagcacgtc ggccaccggc
    11821 agcagcttgt cgggcttggt atgcgccgcg aggtcgccgc cggtcaccag ctccatggcg
    11881 atgtagcccg agtcctcgcc caccaccgcg tcgaggatgg tgacgatgtg cgggtgcttg
    11941 agcttgccgg cgagcgaggc ctcgttgagg aactggctgc ggtacacggt gccgaactcc
    12001 gggtcgcgga acacctcggg ctcgatggtc ttcagcgcca cctcgtggcc cgaaaaggtg
    12061 tccttggcga ggtacaccgt gccggtggct cccttgccga gcatcttctg cacgtcgtac
    12121 ttgccgacct gtaggctcag gccgaggtcc atggcttccg gcaccgccgt ttccttcccc
    12181 agtgggctgc gcagcgccga tgttactgca tggcgcgctt gccgagcgcg cggcgaggaa
    12241 cgagaatcgg gccactccca ccgcccggtc gcccccgcgc atggcccaca aggaagtccc
    12301 ggtcgaatac ctcgagttcg gcatgttcgt cgcccggctc gaccgcccat ggaccgagac
    12361 gccgttcgtc taccagggct tcgtgctgaa gaccgagcag cagctgctcg cgctgaagaa
    12421 gtactgcaag aaggtgtacg tcgacccgga gaagatggag cgggtcgagc cgcccaagct
    12481 cacgctgccg cccaacgcgc gggcggcgat cgccgcgcag gccaaggagg tggtgcgcgg
    12541 caccgaggtc tacaaggaac tcgccagcgt cgaggaggag ctgccgcgcg cgcgcagcgt
    12601 gttccagcag accggcgcgg tggtggaggc gctgtcgcgc agcgtgctcg agggcaacgc
    12661 gctcgacggc aaccgcgcgc gccaggcggt ggtcgagatc accgacagcg tggtgcgcaa
    12721 ccccgacgcc atgatgctgc tggtcaccat gcgcgaaaaa tcggcgcaga ccctgaaccg
    12781 cgccacccag gtctcggtgt acatgacggt gttcggccgc ttcctgcagc tggcgcgcga
    12841 ccggctggag ctcctgggcc tgatgggcct gctgcaggac gtcggcatgc tcaagctccc
    12901 cgccggcctg cccgagcggc gcagcaacct gaccggggcg gaggaggaga gcttcaagag
    12961 ccacatcggg cacagcgtgg agatcctcag ccagacgccg ggcctgccgg cggagctgcc
    13021 gggcctcgcc tcgctgcacc acgagcgcta cgacggcagc ggctatgcgc gcggcctgaa
    13081 gggcgcggcg atctcgcaga tgggcgccat cgccggcatc gtcgacacct tcgacgtgct
    13141 caccgccccc gcgccctacg gcatgcagct gccgccctcc aacgcgctga acgtgctgtt
    13201 ccagaaccgc ggcacgcagt tccacgccgc gctcatcgag cagttcatcc agtgcgtggg
    13261 cgccttcccg gtgggcagcg tggtggagct caacagcggc gaggtcggcg tggtgatcac
    13321 gcagaacctg gtcaagcgcc tgcagccgcg cgtgatggtg gtgcaggacg ccgacggcaa
    13381 cccgatgcac ccgcacaaga tcctcgacct ggtgaacgag cccatggcca acgaggacga
    13441 gccctaccgc atccgccgca cgctcgacca gaccacggtg agcatcaact cgcgcgagct
    13501 gttcctgtag gcggccgcgc gccttggacc ccgccgcgat ccagcacctg atggacgagt
    13561 tcggcgtcga cgccgccgcc atcgcggcgc tggacgcgct cggcgccgcg ctgcgcgcgc
    13621 aggcgcccga ggcgctgctg gcggcgcgcg gcgagagcgc gctgtcggcc gccacctcgc
    13681 cgttcttcct gccgctgctg cccaccggca tgtccgagcc gcagcgcgcg ctggtgaacg
    13741 cgctgtcgct gggcgcgctg gcgcgcgagt cgctcgcgca gctgctgcag gaatgggcgg
    13801 cgacgctcaa cccggaggag cccaccgagc gcgcgcagcg cgactgccgc ccgctgcagg
    13861 acctgctgcg cgcgggcgcc ctgcgcatgt acgccggcga gccggcgcgc tgcgaggccg
    13921 cgctcgccgc cgccgagcgc ctgggcgcgc tgcagtccgc catcatggcc gaggtggcgg
    13981 cgcgcggcgc gcacgcgcgc ccggcggcgg tcgccggcct gggcgcgttc ctgcagcggc
    14041 tcgaggcctg cgtcgcggcg cgcgccgccg acggccagca gctcgcgctg ctgttcgtgg
    14101 actgcggcat catcggccgc atcgacggcg tgtggggcta caccgtgggc gacggcgtgc
    14161 gcgaccggct gagcgcgcgc ctgggcagcg aggtgctgcg cgcgcaggac ctgctcggcg
    14221 accccgggcg cgacgagttc gcctgcgtgc tcgccagcgt gcagagcgcc ggcgtgtcgg
    14281 tgctggcggc ggagaagatc ctgcgcacgc tggatgcgcc gctgtgggtg gacgtggaag
    14341 aggtgtacgc gcggccggcg gtgggcatcg ccctgtatcc ggaggacggc gcgagcggcg
    14401 tggagctgct gcagcgcgcg cgcgtcgcgg cgcgcgccgc gcgcgagcgc cccgagcgct
    14461 tcgccgccta cagcgcggag caggagggcc tggaggccga gctgctggtg tacgagaacc
    14521 gcctgcgcac cgccatcgcc caggacgcgc tggacatcgt gttccagccg cagcgcgacc
    14581 agcgcaacgg ccagatcgtc ggcgccgaga gccagctgcg ctggcgcgac gccagcctcg
    14641 gcgcggtgcc ggtgcagcgc gcgatcgccg ccgccgagtc cgccggactg gtgaacgagg
    14701 tgaccctctg gctggtgaac ggcgcgctgc gcaactgccg cgagttccgc gacgccgccg
    14761 gcctggacct gcgcgtcggg gtgaacctgt cggccaagag cctgcgccag gccgacctgc
    14821 ccgatttcat cgccagcgcg ctgaagacct ggaacctgcg ccccagccgc ctcgcgctcg
    14881 gcatcaccga gaccgcggtg ctggcgcgga gcctggagtc ggtggagacg ctgcagcggc
    14941 tgcgcgaagc cggcgtgcgg ctgtgcctgg acgaccccgg cgccggctac ggctcgctcg
    15001 cctggctctc gacgctgccg ttccacgagc tgcgcgtcga cctcgccgcg gtgggcgacg
    15061 tggccgagtc gccgcagcac ctggcgctgg tgcgcacgct ggtggacttc gcccaccgcc
    15121 tgaagatgga ggtgctggtg tccggcgtgg acgacgaggc caccctggcg aaggtgcagc
    15181 ccctcggctg cgactacctg cagggcgcgc tggtcggcgc gccgctcgac gcggcggcct
    15241 ttgccggccg ccaccgcgcg gaccaatgag gaggaggcaa ggatgcgcaa ccgcctgatc
    15301 tgtactctcg ccgccctggc gctgccgctc gccgcgctcg cgcaatcggg ctacccgcgc
    15361 aagccgatcc tgatgctggt gccgctgcag gcggcgagcg cggtggacaa cgccgcgcgc
    15421 gtcgtcgcgc agaagatgtc cgagaacatg ggccaggcga tcgtgatcga gaaccagccc
    15481 ggcgccgccg ggctgatcgg cgccgagcgc gtggcgaagg cggcgcccga cggctacgtc
    15541 atcggcggct tcaacgacag catcatgacc atgctgccca acgtgcacgc caggatgccc
    15601 tgggacatcc tgcgcgattt cgagccggtg tcgctggtgg cgaccatgga gtgggcgctg
    15661 gtcgcgccgc ccggctcgcc gtaccgcagc gcggcggacc tgatcgccgc ggccagggcg
    15721 cggccgggcg cgctcaacta ttcctccggc ggcaacggca gcccgcagca cgtggcgatg
    15781 gagctgttca agtcgcgcgc cggggtgttc atgacccaca tcccgtaccg cggcgcggcc
    15841 gccgccgcgg tggacaccgc ggcgggccag gtggacgcga gcttctcggg catgccgacg
    15901 gtgttcggga tgatcaaggg cggcaagctc cggctgctcg gcgtcgccag ccccgcgcgc
    15961 tcggcgctgt tcccggaggc gccggcggtg ggcgagtcgc tgccgggctt cgtgttcaac
    16021 tccaatttca gcgtggtggc gcccgccggc acgccgaagg agatcgtcgc gcgcctgaac
    16081 gccgagatcg cgaaggcgac cagcgcgccc gaggtgcgcg acaagctggt cgcgcagggc
    16141 ttcacggtga acgccacctc gccccaggag ctggcgcgct tcacgcgcga gcagctcgcg
    16201 cgctacgcgg cgctgttcaa gcaggccggc atccgcgccg actagggatt gccgatgtag
    16261 tcgcgcttgg ccagctcgac gccgttgtgg cgcaggatgt cgtaggcggt gacggcgtgg
    16321 aagtagaagt tgggctgcgc gaatcccagc aggtactgca ggccctggaa ggtcacttcg
    16381 cgcgggccga gcttgagcac gatcaccttg tcctcgctgc cgtcgagctg cgccgccttg
    16441 acgccgccga tgaacgccac cgtcttcgcg atgcgcgcct tgagctcctc gaaggtcttc
    16501 tcgctgtcct cgtgcttcgg attctccacc ccggccaggc gcgccaccgc gcccttggcc
    16561 gtgtcgcagg cgatggtgat ctggcgcgtg aagggcagca tgtccgggta cagccggaag
    16621 gaagtcagcg ccagcgggtc gatcttcttc gcctcgcaat gcgcctgcgc cttgtcgagg
    16681 atggcggaca ggttgttcag ggtgttggcg aagcgcggcg cgctcgcctg gtacatcgag
    16741 atcggcgtca tgggtttctc cttggggccg gattctagcc gggcgccgtg acgcagtagc
    16801 gcgcagcggg gtttcccggc gatacttcgc cccgccatgg ccgcccccaa cgcgctctac
    16861 gaccccgcgg tcgccctcgc cttcttccgc tccgccggca aggccgagcg ctacgcgccg
    16921 ggcgccacca tcttcgccga gaacgccaag gcgcggcccc tgctgctgca gcaggacaag
    16981 atgtactacg tctacgacgg cgaagtgagc ctgatggcga agaacaaggt catcggcagg
    17041 gtgctggagg gcgaagtgtt cggcgagctg gcgccgctca cgcgcgcacc gcgcagcgcc
    17101 gccgcggtgg cgaagaccgc ctgccgcgtc atctccctgg acgacgagga gttccgcacg
    17161 gcgctgatga agcggcccgc cttcgcgctg atgctgatga gcgtgatggt gacgcgcctg
    17221 cgccagacca tcgccgcgct gcgcgacgcc ggcgtgctgt cggtgagcgg cgcctggaag
    17281 gaggccgcgg tgttcgacaa gcgccagctc gccgacctgg tgcaggggct ggccaacgac
    17341 ccgcagatct actacgagcg cggcgcgatg gtgatcaagg aaggcgccat gggcgcgcgc
    17401 atgtacacgg tgctcgaggg gcgcgtcgcg gtgaccatcg ccggcagcat ggtcgagcgc
    17461 gtcggcccgg gcggcgtgtt cggcgaggcg gcgctggtcg accaggccac gcgcatggcg
    17521 agcgtgcagg ccgaaaccga ctgctcgctg cagccggtgg gccgcgccgc cttcctcgag
    17581 ctggtcaagg tgagcccggt gttcgccgag cgcatgctcg cggcgctggc gcagcgcctg
    17641 cggctgctca ccgcgcgcct ggactagcgc ggtacgcggc gcggcgcgcc tgcggcgtgc
    17701 gccccagcgc gtagtaccag gggtgcggcg tgaccagcgc gtccgcctcg cccagcgcgt
    17761 tggcgcggta gctcgaccag gggtactgcg ccggccggcg cacgatgccg gcgcgcaccg
    17821 ggttcaattc gatatagcgc atgcaggcga gcaggtagcg cggcgcgatc accggcgtcg
    17881 catccacttc gccgccgagc tccagcagca tcgcggtcgc cgccgcgcgg cgcgccgggg
    17941 tgaggagaaa atgggcatgg ctccccatca gcacgtaggc gtgcacgggg cagcctcgct
    18001 cggcggccgc ggcgcgcaag cgctccagat acgccgccgg atcggcggcg ccgccgcgct
    18061 gcaccaggtg cagcgccacg ccgggttcct cggggcagcg cagccggacc atgcgccgaa
    18121 caacgcccgc agcgcggcgg cggttgacgc tctcgcccgc gcccttgacc gcgcagttag
    18181 aatgccccca caccccacgg aggagaccca tgaggcaact cgtcgcagca ctgctgttcg
    18241 tcgcggccgc ggccgcgcaa gcccagtacc cgcagcgccc ggtgcaggtg atcgtgccct
    18301 ggggcgcggg cggcggcacc gacgccaccg cgcgcatcat cgccacgctg ctggagaagg
    18361 aattgaagca gccgttcaac gtggtcaacc gcaccggcgg ctcgggcgtg gtcggccacg
    18421 acgccatcgc caagtcggcg cccgacggct acaccatcgg catgatcacg gtggagatca
    18481 cgatgatgca ccacgtcggc ctcacgccgc tcaagcacac cgactacacg ccgatcggcc
    18541 tggtgaacgc cgaccctgcg ggcgtgcacg tgcgcgtgga ctcgccctac aagtcggtga
    18601 aggacctgct cgccgcgatc aaggccagcc cgggcaagat gaaagcctcc ggcaccggcc
    18661 agggcggcat ctggcacctc gccatcgccg gattgctgaa ggaccagggc atcgacccga
    18721 acgcgctgcc ctgggtgccc tccaacggcg ccgcgcccgg gctgcaggac atggtggcgg
    18781 gcggcgtgga agtggtgccc tgctcggtgc ccgaggcgcg cggcatgatc gacgcgggca
    18841 aggtgcgcgc gctggcgatc atggacgcca acccgccggc gctctatccg aacgtgccca
    18901 cgctgaagaa ggaactgggc accgactggg cgatcgccgc ctggcgcgtg atcgccgcgc
    18961 ccaggggcat tccggccgaa gcgcagaaga cgctgaccac ggcgctgaag aaggtctacg
    19021 actccaagga gtacaaggac ttcatggcga gccgcggctt cggcgtgatc tgggccgatc
    19081 cggcggccac cgcgaaattc atggcggcct cggacgcgaa cctcggcgcg gcgctcaagg
    19141 cagtaggcct ggccaagtag cttgaaggtc aacgatgcgc tgagcggcgc ggggctcgcc
    19201 gctctgggcg gggtcgtcct ctggcacatc caggggttcc ccgccatgcc gggccagaaa
    19261 ttcggcccgg cctggttccc cggggcgatc gcgatcggcc tgctcgcctg cggcctgctg
    19321 ctgatgctgc gcgccgagcg cgggccctgg atcgcgctgc cgccctggac ccggcgcgcg
    19381 cggccgctcg ccggcgtcgc cgcggtggtg ggcgggctgc ttgcctacgt gctcgccgcc
    19441 gacaggctcg gcttccacct caccgggttc gcgctgctcg cgctctggtg ccggatcctg
    19501 ggcgccggct ggcgggcgtc ggtcgcggtg tcggcgatcg cgaccctggc gatccacctt
    19561 tcgttctaca agctgctgcg catcccgctg ccctggggcg tgctcgagcg cttcgccttc
    19621 tgagatgagc gccgagatcc tcgcgcaggc gctcgcgcag gtgttcgatc cctacgtgct
    19681 gtgggtgatc ctcggctcgg cggtatttgg tctcttcgtc ggcgcggtgc cggggctcac
    19741 cgccaccatg gccaccgcgc tgctggtccc ggtgaccttc ttcatgccgc cggtgccggc
    19801 gatcgcttca atcgtcaccg ccaccgcgat ggcgatcttc gccggcgaca tcccgagctg
    19861 cttcctgcgc atgcccggca cgccggcctc cgccgcctac accgacgagg cctacgccat
    19921 gaccaggaag ggccagggcg agctcgccct gggcgcgagc ctggtgttct cggtgatcgg
    19981 cggcctgttc ggcaccgcgg tgctgatcct cgcggcgccg gcgctcgccg aggtggcgct
    20041 caggttctcc tccttcgaat acttctggct ggtggtgctg ggcctggcga gcgcggtgtt
    20101 cctcgccctc gccaacccgc tgaagggcat cgtgtcgctg ctcctcggcc tgctggtcgc
    20161 ctcggtcggc ctggacaacc cggcgggctt cccgcgcttc accttcggca acgccgagct
    20221 ggccggcggc ctcacgctga tcccgatcat gatcggcatg ttcgcggtgt cggaagtgat
    20281 ccgcaacgtg gcggcgatcg agcggccctg ggagatggcg caggcgacga tcggcaacgt
    20341 gttccagggc atgtgggggc tgctgcggcg ctatcccctg cagacgctgc gcggcagcgt
    20401 gctcggcacc gccatcggcg cgctgcccgg cgcgggcgcg gacatcgccg cctggatgtc
    20461 ctacgccgtg tcgaagaagt tctccaggga acgcggcaag ttcggcagcg ggcacgtcga
    20521 gggcatcatc gaggccggcg cctccaacaa cagcgcgctc gccggcgcct ggatcccggc
    20581 gctggtgttc ggcatccccg gcgactcgat caccgccatc gtcatcggcg tgctctacat
    20641 caagggcctg aacccggggc cgacgatctt catgaccaac gccgtggcga tctacgccat
    20701 cttcatggtg ttcatcctcg ccaacctgat catgctgccg ctgggcgtgg ccgccatcaa
    20761 gggcgccaag cagatgctgc gcgcgccgcg cgagctgctg ctgccggtga tcctgctgtt
    20821 ctgcatcgtc ggctccttcg ccatcaacaa ctccgccttc ggcgtgctgc tgatgctcgc
    20881 cttcggcgtc gccgcctacc tgatggagga gaacggcttc ccggtggcgc cggcgatcct
    20941 cggcatggtg ctgggcgcga tgctggagga gaacttcatc agctcgatga tcaaggccga
    21001 cgggcggctg ctcgccttct tcgagcggcc gatcgccggc ggcctcggcg cgctggcgat
    21061 cgcggtgctg ctggtgccgg ttttgcgcgc ccggctgcgc cgcggcgcgc cggcgcggta
    21121 ggatgctcgc ctgaccgaac agcgaggacc gccatgagcg ccccccagaa acccgccgcc
    21181 gccgcgcccc acgacccgat ccaggagatg gcggagaagc tctacgtcgc gctgtgcgcc
    21241 cgcgtctaca gcgcggccgg cgagaagccg cagcccaagg cggtggcgca gctgtgcttc
    21301 aagctcgccg agaccttcca ggccgccaac tacgaattca accccaccgc cgtcgccgcg
    21361 cgcgcggcga aggacaaggc ctcggtgagc ctcgattcgg tgaacatcga cttcggcgcg
    21421 gtcggcaagt cctagccttg cgcgtcggca tcatcggcgc cggcttcgcc ggcctcggcg
    21481 cggcgatccg cctgcggcag gccggcttca ccgacgtcac cgtctacgag cgcgccgccg
    21541 agctgggcgg cacctggcgc gacaacacct acccgggctg cgcctgcgac atcccctcgg
    21601 tgctgtattc gttctccttc gcgcccaacc cggaatggac gcgcatgtat ccctcgcagc
    21661 cggagatctg ggactacctg cggcgcctcg cgcgcgcgca cgggctgctg gagcggatcc
    21721 gcttcggcgc cgagatgcgc gcggcgcgct gggaagaagc ctcgcgcacc tggcgcctgt
    21781 ccttcaagga cggcagcgag gcgagcgtgg acgtcctcgt cagcgcctcc ggcttcctca
    21841 acaagcccgc gctgccgcag ctccccggcc ttggcggctt cgaaggcgcc gccttccact
    21901 cctcgcagtg gcgccacgac gtggacctcg ccggcaagcg cgtggcgctg gtgggcaccg
    21961 gcgcgagcgc gatccagatc ctgcccgaga tcgcgaagac cgcggcgaag gtgcatgtgt
    22021 tccagcgcac gccgccctgg gtgctgccgc ggctggaccg ggcgttctcg gcgcgcgagc
    22081 ggcgctggtt ccgccgcctg cccttgctgc agcgcctgtc gcgctggcgc ctctacctgc
    22141 gccaggaact gctggtgacc gggttcctcg gcggcgcctg gatccagaag cgcttccgcc
    22201 agtacgccgc ggggctcctg gagcgccagg tgcccgacgc cgcgctgcgc gcgaagctca
    22261 cccccgccta cgcccccggc tgcaagcggc tgctggtgtc caacgactgg tacccggcgc
    22321 tgcagcggcc caacgtcgag ctcctcgccg agccggcggc gcggctggaa ggccggcgca
    22381 tcgtcggcgc ctcgggcgcg gcgcgcgagg tggacgcggt ggtgttcgcc accggcttca
    22441 tcgccaccga attcgtcgcg ccggtgaagg tgcacggccg cggcggcgtg gaactcggcg
    22501 agctgtggaa ggacggcgcc gcgagccacc tcggcatcag cgtggcgggc tttcccaacg
    22561 ccttcttcat cgtcggcccg aacaccggcc tggggcacaa ctcgatcatc ttcatgatcg
    22621 aggcgcagct gaactacctc gtcggcgcgc tcaagtccgg ggcccaggtc ctggagctgc
    22681 tgccggaagt gcagtcgaag agctacgccg ccgtgcagcg gcgcatgaag aagaccgtgt
    22741 gggcgtcggg ctgccgcagc tggtacctct ccgccgacgg gcgcaacgac acgctgtggc
    22801 ccggctcgac gctggaatac tggtggcgca cgctgcgctt cgacgccgcg agctaccgct
    22861 caggctagcg ccgccgccac cgcgtcgccc atcgccgcgc agcccacctg gcgcttgccc
    22921 ggctgcatca tgtccgcggt gcgcagcccc tgcgccaggg ccttgcgcac cgcgcgctcg
    22981 atgcgctccg ccgcatcccg gtcgccgaag ctcatgcgca gcatcatcgc cgccgagagg
    23041 atcgcggcga gcgggttggc gaggtccttg ccggcgatgt cgggcgcgct gccgtgcacc
    23101 ggctcgtaca ggccgaagcg gttctcgccg atcgagcccg agggcagcat gccgatggag
    23161 cccgtgagca tggcggcggc gtccgacagg atgtcgccga acatgttgcc catcaccatc
    23221 acgtcgaact gcttcggcgc gcgcagcagc tgcatcgagg ccgcgtcgac gaacaggtgc
    23281 gtcagcgcca cgttcggata ctgcctgccg atgcgcgcca ccgtctcgcg ccacagctgc
    23341 atggtctcca gcacgttcgc cttgtccacc gagcagacct tcctgcggcg ctcgcgcgcg
    23401 gtgcggaagg cgacgtgcgc gatgcgctcg atctcgggct cggtgtagcg catggtattc
    23461 acgccttcgc gccggccaga gtcgccgacg cggatgccgc gcggctcgcc atagtagatg
    23521 tcgccattca gctcgcgcag gatgatgagg tcgagcccct gcaccagctc gggcttgaga
    23581 gacgaggcgc cgaccagctc gggaaacagg aacgccggcc ggaaattggc aaacagcttg
    23641 agctcgcgcc gcagcgtcag caggccgagc ccggggcgct tgtcgcgcgg tccgctctcg
    23701 tcgcccggag tgcccaccgc gccgaacagg atggcgtcgg ccttgcgcgc cagctcgagc
    23761 gtcgccggcg gcagcggcac gccggccgcg gcgaccccgg cggcgccgat cggcgcttcg
    23821 gtgaacttcg cgtcgcttcc cgcgagcgcc tgcagcactt tgaccgcctc ggcggtgact
    23881 tccttgccga tgccgtcgcc cggcatgacc gcgatgttca tccgtcgtat tatcccgcgc
    23941 tcacccatgg cgaaaaagaa accggaagcg aaacaccca
//
LOCUS       DASRIE010000159         4995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2237579, whole genome shotgun sequence.
ACCESSION   DASRIE010000159 DASRIE010000000
VERSION     DASRIE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237579"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFB53_06930"
     CDS             complement(<1..421)
                     /locus_tag="VFB53_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN86503.1"
                     /translation="MRAEIGKALIGQPEVVDAVLCALLAGGHVLVEGVPGLGKTLLVK
                     ALARTISGSFGRIQFTPDLMPANVTGHTLYDPKNQVFTTRRGPVFVNLLLADEINRAP
                     AKTQAALLEVMQEAQVTIEGEAHKVEPPFMVLATQNPI"
     gene            complement(472..1572)
                     /locus_tag="VFB53_06935"
     CDS             complement(472..1572)
                     /locus_tag="VFB53_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZN86504.1"
                     /translation="MKAKLVLLLGLAALIGLSVLWFRANFELVPVTERVGATGEARLR
                     QFLAAERFAERMGLAAREARSLPALDALPAAGVLMMPARRQALTVERLADLVRWAERG
                     GHLIVEAEFLGVPDPLLGQLGVQRADAPPAKSLVAEVGGRRLQLLVASGMKLVAPAAE
                     IRLRAGDQLISFGRGRGAVTVATSLDFARNPSIGALDHAEFLWHLLRLTPGRELVVYW
                     APERLSLWGFLKQNAAAALAASALLLALWLWSIAPRFGPLAPEAPPGRRRLLDHLRAS
                     GRYYWAKGLRSRLVVAARDAALRRLARAQPDFAAAPHSEKIARLSSLAGISKEEAARF
                     LSAAGATRGADFIRVMQHAQRVHSALEKGTKP"
     gene            complement(1569..2972)
                     /locus_tag="VFB53_06940"
     CDS             complement(1569..2972)
                     /locus_tag="VFB53_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86505.1"
                     /translation="MQPASIAISLRRRSPWEAMDLGFTMLRRWWRPVYSAHALVFVPL
                     NALVLLAGWLSGMLWLAMLAVWWLEPLYDRVVLHVLSRAVFGDVPSSGFLRQPSQYAS
                     GLLWALTFDRFDLARSFNLPVRQLEGSRGRERRARKAVLGRRTRSYAVWLTIVWVHFE
                     GLLMWSADGLASLLLPAQAERARGDDGSFFTDLFEALSRASLEDALVYAVVIFVLEPF
                     YVASGFALYLNRRTLLEGWDIEVALRRIAEKHVAVLLIVVFTFGFCVPSSAQEKDPRK
                     EIAEVLKAKEFGYERTVERWQAKKKPDEPQKQDRSGWLALGYALAKAAEVLLWIAAGA
                     LAAYALWWAARMLPRRRAPPAEPYRPPAALFGMELAPETLPADVGAAAAALAREGRLR
                     EALSLLYRGALSELVHRRGVQLLASHTEGDVLRLSGELPYLHDLVQSWKACAYARRVP
                     PSAEIERLAHDYRAAFA"
     gene            complement(2959..3912)
                     /locus_tag="VFB53_06945"
     CDS             complement(2959..3912)
                     /locus_tag="VFB53_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZN86506.1"
                     /translation="MKQAPFELAHAREWGEFEAFLERKSAFDPAEMPARFRRLCQHLA
                     LAAERRYSADLVDRLNRLALRGHHALYANRRRESQQVLGFVLAGFPQLVRREWRLVAA
                     AALLFFGPLLALIALLQQFPDFVHYLLSPDQIASFHQMYDPANRRIGMREADTNVMMF
                     GFYIWNNVRIGFQTFAGGLLAGVGSAWFLGANGVVIGAVAGYLTQAGFGETFWSFVAG
                     HSALELTAIVLSGAAGLRLGLGVIAPGNLTRKAALVAAAKPAVRIMYGAALMFLGAAF
                     VEAFWSPLTEIPFDVKIGAGIAAWAVLLAYFLFAGRARAAR"
     gene            complement(3909..4595)
                     /locus_tag="VFB53_06950"
     CDS             complement(3909..4595)
                     /locus_tag="VFB53_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN86507.1"
                     /translation="MLDTTRRVATPEGIELTLRLAGPVPRALAWSVDLAIRIAIVLTV
                     MLLASQLGRAGWGVVLLAAFFVEWLLPAWFEAQMGGQTPGKRLFGLAVLNDDGTPLRW
                     PGALTRNLLRAVDFLPFMYAFGLVAMLLNRDFKRLGDLAAGTVVVYLSAATEPGRKIP
                     EAAPIPPPVALDLDEQRAVLDLAERSAGLTRERVEELAALPTPLVGALEGERAAARLL
                     GMANYLAGRQ"
     gene            complement(4635..>4995)
                     /gene="rho"
                     /locus_tag="VFB53_06955"
     CDS             complement(4635..>4995)
                     /gene="rho"
                     /locus_tag="VFB53_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001137.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN86508.1"
                     /translation="FGAARNVEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGNMEVH
                     LDRRMHEKRIYPAINVNRSGTRREELLIEAPLLQKVWVLRKLLFPMDELEAAEFLIDK
                     LRATKSNAEFFDSMRRG"
BASE COUNT          801 a         1781 c         1753 g          660 t
ORIGIN      
        1 cgatcgggtt ctgcgtggcg agcaccatga acggcggctc caccttgtgc gcctcgcctt
       61 cgatcgtgac ctgcgcttcc tgcatcacct cgagcagcgc cgcctgggtc ttcgccggcg
      121 cacggttgat ctcgtcggcg agcagcaggt tcacgaagac cggcccgcgc cgggtggtga
      181 acacctggtt cttcgggtcg tacagcgtgt gcccggtcac gttcgccggc atcaggtcgg
      241 gcgtgaactg gatgcggccg aagctgcccg aaatggtgcg cgccagcgcc ttcaccagca
      301 gcgtcttgcc caggccgggc acgccctcga ccagcacgtg gccgccggcg agcagcgcgc
      361 acagcaccgc gtcgaccacc tccggctggc cgatcagcgc cttgccgatc tcggcgcgca
      421 tcgcgcccgc gaccttggcc gcctggttga tctgctcgga ggtgggcttg ttcatggctt
      481 ggttcccttt tccaatgcgc tgtgcacgcg ttgcgcgtgc tgcatgaccc tgatgaagtc
      541 ggcgccgcgc gtcgcgccgg ccgccgacag gaagcgggcg gcttcctcct tcgaaattcc
      601 ggcgagcgac gagagcctgg ctatcttttc gctgtgcggg gcagcggcga aatccggctg
      661 cgcgcgcgcg aggcgccgca gcgcggcgtc gcgcgcggcg accacaaggc gcgagcgcaa
      721 tcctttcgcc cagtagtagc ggccgctcgc gcgcaaatgg tcgagcaggc gccgccgtcc
      781 gggcggcgcc tcgggcgcga gcgggccgaa gcgcggcgcg atgctccata gccacaaggc
      841 gagcagcagc gcggacgcgg cgagggcggc tgccgcgttc tgcttgagga agccccacag
      901 cgaaaggcgc tccggcgccc agtacacgac cagctcgcgc ccgggggtca gcctgagcag
      961 gtgccacagg aactcggcat ggtccagcgc cccgatcgag gggttgcgcg cgaaatcgag
     1021 cgaggttgcc accgtgaccg cgccgcggcc cctgccgaac gagatcagct ggtcgcccgc
     1081 gcgcagccgg atctccgccg ccggcgcgac cagcttcatg ccgctcgcaa cgaggagctg
     1141 gagcctgcgc ccgccgacct cggcgacgag cgacttcgcc ggcggcgcat cggcgcgctg
     1201 cacgccgagc tggccgagga gcggatcagg cacgccgagg aattcggcct cgacgatcag
     1261 gtggccgccg cgctccgccc agcgcaccag gtccgcgagc cgctcgacgg tgagcgcctg
     1321 cctgcgcgcc ggcatcatca ggacgcccgc cgccggaagc gcgtccagcg cgggcagcga
     1381 gcgcgcctcg cgcgccgcga ggcccatgcg ctcggcgaag cgctccgcgg cgaggaactg
     1441 gcgcagccgc gcctcgcccg ttgcgcccac ccgctcggtg accgggacca gctcgaagtt
     1501 cgcccggaac cagagcacgc tcagcccgat cagcgccgcg agcccgagca gcaggacgag
     1561 tttcgccttc atgcgaaggc cgccctgtag tcgtgcgcca gccgctcgat ctcggcggac
     1621 ggcgggacgc ggcgcgcgta ggcgcacgct ttccacgact gcacgagatc gtggagatag
     1681 ggcagctcgc cggagaggcg cagcacgtcc ccctcggtat ggctcgcaag gagctgcacc
     1741 ccgcgccggt gcacgagctc cgagagcgcg ccgcgataga gcaggctgag cgcctcgcgc
     1801 agcctgcctt cgcgcgcaag cgcggccgcg gccgcgccga cgtcggcggg cagcgtttcg
     1861 ggcgcgagct ccatgccgaa gagcgccgcc ggggggcggt acggctcggc cgggggtgcg
     1921 cggcgccgcg gcagcatgcg agccgcccac caaagcgcat acgccgccag cgcgccggcc
     1981 gcgatccaca gcagcacctc ggccgccttg gcgagcgcgt agccgagcgc aagccagccg
     2041 ctgcggtcct gcttctgcgg ctcgtcgggc ttcttcttcg cctgccaccg ctcgaccgtg
     2101 cgctcgtagc cgaactcttt cgccttcagc acctcggcga tttcctttct tgggtctttc
     2161 tcctgcgcag acgaaggcac gcagaaacca aaggtaaaaa cgacgatcag cagcacggcc
     2221 acgtgctttt cggcgatgcg gcgcagggcg acctcgatgt cccagccctc gagcagcgtg
     2281 cgacggttca ggtagagcgc gaacccggag gcgacgtaga acggctcgag cacgaagatg
     2341 accacggcgt acaccagcgc gtcctcgagc gaagcgcggc tcagcgcctc gaacaggtcc
     2401 gtgaagaaag agccgtcgtc gccgcgcgcg cgctcggcct gcgcgggcag cagcagcgac
     2461 gcgagcccgt cggccgacca catcagcagg ccttcgaagt gcacccacac gatggtgagc
     2521 cacaccgcat agctgcgcgt ccttcgcccg agcaccgcct tgcgcgcgcg gcgctcgcgc
     2581 ccgcggctgc cttcgagctg gcgcaccggc aggttgaacg agcgcgcgag gtcgaagcgg
     2641 tcgaacgtga gcgcccagag gaggcccgag gcgtactgcg acggctgcct gaggaagccg
     2701 ctcgagggca catccccgaa taccgcgcgc gacaggacgt gcagcacgac gcggtcgtag
     2761 agcggctcga gccaccacac ggcgagcatc gcgagccaca gcatgccgct cagccacccg
     2821 gcgaggagca ccagcgcgtt gagcggcacg aacacgagcg cgtgagccga gtagaccggg
     2881 cgccaccagc gccgcagcat ggtgaagccg aggtccatcg cctcccacgg cgagcgccgg
     2941 cgaaggctga tcgcgatgct agcgggctgc acgcgcgcgc ccggcgaaga ggaagtaggc
     3001 gagaagcacg gcccacgccg cgatcccggc gccgatcttc acgtcgaaag gaatctcggt
     3061 gagcggcgac cagaaggcct cgacgaacgc agcgccgagg aacatgagcg ccgcgccgta
     3121 catgatgcgc accgcgggct tcgccgccgc gaccagcgca gccttgcgcg tgaggtttcc
     3181 cggcgcgatc acgccgagcc ccaggcgcaa gccggccgcg cccgaaagca cgatggcggt
     3241 gagctcgagc gccgagtgcc cggcgacgaa ggaccagaag gtctcgccga accccgcctg
     3301 cgtcaggtag ccggcgaccg cgccgatcac cacgccgttc gcgccgagga accaggccga
     3361 cccgaccccg gccagcagcc cgccggcgaa ggtctggaag ccgatgcgca cgttgttcca
     3421 gatgtagaag ccgaacatca tcacgttggt atcggcctcg cgcatgccta tgcgccggtt
     3481 cgccgggtcg tacatctggt ggaagctcgc gatctgatcg ggcgagagca ggtagtggac
     3541 gaaatccgga aactgctgca gcagcgcgat cagcgcgagc agcggcccga agaacagcag
     3601 cgcggcggcc gcgaccagcc gccattcccg gcgcacgagc tggggaaagc cggcgagcac
     3661 gaagccgagg acctgctggg attcgcggcg ccggttcgcg tagagcgcgt ggtggccgcg
     3721 cagcgcgagc cggttcaaac ggtcgaccag gtcggcgctg tagcgccgct ccgccgcgag
     3781 cgcgaggtgc tggcacaggc ggcggaagcg cgccggcatc tcggcggggt cgaaggcgct
     3841 tttcctttcg agaaaagcct cgaactcgcc ccattccctt gcatgcgcga gctcgaacgg
     3901 cgcctgcttc actgccgtcc agccaggtaa ttcgccatgc cgagcaggcg cgccgcggcg
     3961 cgctcgcctt ccagcgcgcc gacgagcggc gtcggcaggg cggcgagctc ctcgacgcgc
     4021 tcgcgcgtaa ggcccgcgga gcgttcggcg aggtcgagca ccgcgcgctg ctcgtcgagg
     4081 tcgagcgcca ccggcggcgg gatcggcgcg gcctccggga tcttgcgccc cggctccgtc
     4141 gcggccgaca ggtacaccac caccgtcccg gcggccaggt cgccgaggcg cttgaagtcg
     4201 cggttgagga gcatcgcaac caggccgaag gcgtacatga aaggcaggaa gtcgaccgcg
     4261 cgcagcaggt tgcgcgtgag cgcacccggc cagcgcagcg gggtgccgtc gtcgttcagc
     4321 accgcgaggc cgaagagccg cttgcccggg gtctggccgc ccatctgcgc ctcgaaccac
     4381 gccggcagca gccactcgac gaaaaacgcc gccagcagca ccacgcccca gccggcgcgg
     4441 cccagttgcg aggcgagcag cattaccgtc agcacgatcg cgatgcggat cgcgaggtcg
     4501 accgaccagg cgagcgcgcg cggcaccggg ccggcgaggc gcagcgtgag ctcgattcct
     4561 tcgggtgtcg cgactcgacg ggtggtgtcg agcatgcggt ttcccctccc agggaaaccg
     4621 attctagttt cttcttatcc gcgccgcatc gagtcgaaga attcggcgtt cgacttggtg
     4681 gcgcgcagct tgtcgatcag gaattccgcc gcctcgagct cgtccatcgg gaagagcagc
     4741 ttgcgcagca cccacacttt ctgcaggagg ggcgcctcga tcagcagctc ctcgcgccgc
     4801 gtgccgctgc ggttgacgtt gatcgccggg tagatgcgct tctcgtgcat gcggcggtcg
     4861 aggtgcacct ccatgttgcc ggtgcccttg aattcctcgt agatcacgtc gtccatgcgg
     4921 ctgccggtgt cgatcagcgc ggtggcgatg atggtgagcg agccgccttc ctcgacgttg
     4981 cgcgccgcgc cgaag
//
LOCUS       DASRIE010000160         9960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2781327, whole genome shotgun sequence.
ACCESSION   DASRIE010000160 DASRIE010000000
VERSION     DASRIE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781327"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFB53_06960"
     CDS             complement(<1..195)
                     /locus_tag="VFB53_06960"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZN86509.1"
                     /translation="MQKPELSIEFFPPQTPDGVEKLAATRAKLAKLNPAFFSVTYGAG
                     GSTRERTFATVKEIAAGGCEA"
     gene            complement(206..1621)
                     /gene="ahcY"
                     /locus_tag="VFB53_06965"
     CDS             complement(206..1621)
                     /gene="ahcY"
                     /locus_tag="VFB53_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZN86510.1"
                     /translation="MNAAVMNAKSGDFRVADLKLAGWGRKEIAIAESEMPALMAIRDE
                     YARSRPLKGARITGSLHMTIQTAVLIETLKALGAEVRWASCNIFSTQDHAAAAIAAGG
                     TPVFAVKGETLDEYWDYTHRIFEFGDKGPNMILDDGGDATLLVHLGQKAERDPSVLAS
                     PSSEEETSLFNAIRKKLAASPGWYTKVGKDIKGVTEETTTGVHRLYEMAKEKRLLFPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVALIAGYGDVGKGCASALRAL
                     SAQVWVTEIDPICALQAAMEGYRVVTMDYAADKADIFVTATGNYHVITHDHMAKMKDQ
                     AILCNIGHFDNEIDVASVEGYEWEEIKPQVDHVIFPDGKRIILLAKGRLVNLGCATGH
                     PSYVMSSSFANQTIAQIELFTRTADYPVGVYTLPKHLDEKVARLQLKKLNAQLTELRA
                     DQAAYIGVPVEGPYKSTHYRY"
     regulatory      complement(1661..1737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1800..2510
                     /locus_tag="VFB53_06970"
     CDS             1800..2510
                     /locus_tag="VFB53_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86511.1"
                     /translation="MPAAQPSVFASVAFLKIHDFGRRPVVEQTRLRAQLEAAVAVTLH
                     ALAEPSRVVLDAADGVALVVFGDPRGALRIAERALSAGAAGLPLAIGITHGAVRQTGE
                     GAGEGLLGDGIAVAAAIAELAGPQKLLLTREFRAALADAAPGAEAAVAGAGTHTDASL
                     RSHELYRPDAFAARRRARRYAAATVLAALCLVGSGAGYRASIEGPRAYAGRVAADLKG
                     AAAKAQERALRLLQQTGR"
     gene            2510..3658
                     /locus_tag="VFB53_06975"
     CDS             2510..3658
                     /locus_tag="VFB53_06975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN86512.1"
                     /translation="MAKKRKENQIGRYRILGELGRGAMGVVYRAEDPSLDRVVALKTI
                     SLANADEDRKSYEKRFLLEAKAAGKLTHPNIVTIFDYGEEEDLAYMAMELLEGADLRT
                     RMRQGEIAPLEAVEIALQVADGLGFAHETGVVHRDIKPGNIMLLERGAVKIMDFGIAR
                     MRHADHKTSTGMVLGTPRYMSPEQIAGQPVDQRSDIFSLGTVLYEMLTRTMLFAGDDV
                     NQIAHNLANAEPVPPSRSNPAIPQIVDFITARALKKDPAVRYQDAYEMAADLRDALAE
                     MRGRAPAPRNGDAEATHTMKLEPGAHKPPLAPAASAIARDTRLPLSRLFDSEAALERL
                     AVPKHSARLARAPRQVGLLRRILRDRPVRRLCAVALVAALLGSWIAIG"
     gene            3756..4859
                     /locus_tag="VFB53_06980"
     CDS             3756..4859
                     /locus_tag="VFB53_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86513.1"
                     /translation="MQKIVPGRRPFIVRALGALASLPLLQACMGGAGGQARSAPAAPG
                     SFDFGVVGDIPYTRAQEAEYLRVMQDINSRDLEFVAHIGDTMFDPRPYERDPSLARTP
                     LTDENYAYVLGTFQSCRHPLVLTPGDNDWSDAVQFQKVKVDPLERLAKLRATFYPKGR
                     SLGQRTMAVESQAQDPVYATYVENLTWAVRGLRFATLHIVGSNDNARTSMDEHKARQA
                     AVIAWMRKAFAAAKAGGSLGLVLITHANPDFENRWTRSYASRYARSVRGAEAPKAPEA
                     TPYDPFLDALIEQMQGYDKPVLYVHGDTHIFRIGNPLMNPKTQRFFENLTRLETFGWP
                     DSAWVRVTANPSNPHLFEIHPEYVRGNSANYKA"
     gene            complement(4864..6021)
                     /gene="metK"
                     /locus_tag="VFB53_06985"
     CDS             complement(4864..6021)
                     /gene="metK"
                     /locus_tag="VFB53_06985"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZN86514.1"
                     /translation="MSEFLFTSESVSEGHPDKVADQISDAVLDAILSQDKTSRVAAET
                     LVKDNLVVLAGEITTGAKVDYQSVVRRTVKRIGYDDPVIGFDTHTCTVIVAYGEQSKD
                     IAQGVDEGKGLDLDQGAGDQGLMFGYACDETPSLMPMAIYLSHRLVERQSELRRDGRL
                     AWLRPDAKSQVTIRYANGKPDSIDTVVLSTQHKPGMTHKQIEEAVIEQVIKPVLPKNL
                     LKGKVKYLVNPTGSFEIGGPKGDCGLTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLASKCLVQVSYAIGVAQPTSVMVTTQGTGKIADEKLSQLVLA
                     NFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWERTDKVLALKQAA"
     gene            6057..6875
                     /locus_tag="VFB53_06990"
     CDS             6057..6875
                     /locus_tag="VFB53_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN86515.1"
                     /translation="MMQLAARLPLRVLHALGSLLGWAMYALSPTYRRRLRANLEQAGY
                     TDARTRREAIAEAGKLITESPKLWLRPREEVLKLVHGFEGLEEAARVHAAGRGIVFLT
                     PHLGCFEITAQAAAERVPLTVLYRPPKVDWLRPLMERGRGGRNMTLASADLGGVRQLL
                     SALKRRETVGILPDQVPGEGEGEWAEFFGRPAYTMTLAAKLIARGDVACFLAVGRRLP
                     RGAGYALRMAPLPSALPGESGTRRINRALEAAIRECPGQYLWGYNRYKKPRGGR"
     gene            6872..7741
                     /locus_tag="VFB53_06995"
     CDS             6872..7741
                     /locus_tag="VFB53_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZN86516.1"
                     /translation="MSRVVFFLMWLVHFLPLRIQAAIGNAAGALLYWLIPERRRVTRI
                     NLAKCFPQMDPAARERLARAHFRSFCRGFVEHGLLWWAPRERIERLVRVEGLEHLRAL
                     GAAPALLFVPHFAGLDAGFTRLCMELDMVGIYSRQKDARFGRLLALGRGRFGNQRQVS
                     RQEGVRAAITAMLREHRAFYYLPDQDHGPLNAVFVPFFGVPAATVPGLSRIAKLSGAR
                     VLPCVTRMLPGGQGYLVRIEPPWEGFPTADLLADTRRMNAHIEAVCREIPEQYLWMHK
                     RFKTRPEGEARFY"
     gene            7755..8708
                     /locus_tag="VFB53_07000"
     CDS             7755..8708
                     /locus_tag="VFB53_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN86517.1"
                     /translation="MKKTLRAAVIGVGYLGQFHAEKLAALQGVELVAVVDADAARAKQ
                     VAAKHACAALADPGALAGRVDLVSIAVPTDRHHAVALPLLEAGVHALVEKPIARSLEE
                     ADAMLAAARRGGALLAVGHLERFNPAFRALAAASTKPLFIDCERLSAFKQRGIEVDVV
                     LDLMIHDLDLVLSLARGEIAQISACGFQVLTDSIDIANVRIEFADGCVANLSASRVSQ
                     SPVRKLRVFQGDGYASADLQAGKLRMVRRDARKGGIVEDEATFDDRDALRAEIEGFVD
                     AVRGGRRPPVAGEDGRKALALALEVGRLVRARLERFKAVAA"
     gene            8705..9799
                     /locus_tag="VFB53_07005"
     CDS             8705..9799
                     /locus_tag="VFB53_07005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZN86518.1"
                     /translation="MKVPILDLAAELREVGAEVRAALERVLASGHFILGPEGAALERE
                     IAALLGVPHAVACNSGTDALHLALRAAGIGPGDEVVTPAFTFIATAEAIAYVHARPAF
                     ADVDPATFNMTAATLERALTPRTRAAIVVHLFGQCAEMPPIAELCRKKGLVLIEDCAQ
                     SIGADWDGRQSGAWGDFGCFSFYPTKNLGAYGDAGLVTARTAQHDAALRMLRHHGSRV
                     TYSHEVIGLNSRLDEMQAAILRVKLKHLARFSAARRAHAARYRELLAGAALRLPAEHG
                     HGTHIYHQFTLRLARRDAAKQALAEAGVASGVYYPIPLHRQPVFAKEFGALSLPAAEA
                     AAREVLSLPIYPQLADEQLRHVAASLRACL"
     gene            9814..>9960
                     /locus_tag="VFB53_07010"
     CDS             9814..>9960
                     /locus_tag="VFB53_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZN86519.1"
                     /translation="MEGAGNDFVVLDATAKPFALSGEQLRRLADRHFGVGCDQVLVVE
                     KARQP"
BASE COUNT         1426 a         3481 c         3518 g         1535 t
ORIGIN      
        1 ggcctcgcag ccgccggcgg cgatctcctt gacggtcgcg aacgtgcgct cgcgcgtcga
       61 gccgccggcg ccataggtca ccgagaagaa agcaggattg agcttcgcga gcttcgcacg
      121 ggtggcggcg agcttctcga cgccgtcggg cgtctgcggc ggaaagaact cgatcgaaag
      181 ttcaggcttc tgcatgacgc cctcgtcagt agcggtagtg cgtcgacttg taggggccct
      241 cgaccggcac gccgatgtag gcggcctggt ccgcgcgcaa ctcggtcagc tgcgcgttga
      301 gcttcttcag ctgcagccgc gcgaccttct cgtcgagatg cttcggcaag gtgtagacgc
      361 ctaccgggta gtccgcggtg cgagtaaaga gctcgatctg cgcgatcgtc tggttcgcga
      421 acgacgacga catcacgtag ctcgggtggc cggtcgcgca gccgaggttc acgaggcggc
      481 ccttggcgag caggatgatg cgcttgccgt ccgggaagat cacgtgatcg acctgcggct
      541 tgatctcttc ccactcgtag ccctcgaccg acgcgacgtc gatctcgttg tcgaagtggc
      601 cgatgttgca gaggatcgcc tggtccttca tcttcgccat gtgatcgtgc gtgatcacgt
      661 ggtagttgcc ggtcgcggtc acgaagatgt cggccttgtc ggcggcgtaa tccatggtaa
      721 cgacccggta gccttccatc gcggcctgca gcgcgcagat cgggtcaatc tcggtcaccc
      781 acacttgcgc ggacaaagcg cgcagcgcgc tcgcgcagcc cttgccgacg tcgccgtagc
      841 ccgcgatcaa cgcaaccttg cccgcgatca tgacgtcggt ggcgcgcttg atgccgtcca
      901 ccagcgactc gcggcagccg tacaggttgt cgaacttcga cttggtcacc gagtcgttga
      961 cgttgatcgc cgggaacagc aggcgctttt ccttcgccat ctcgtacagg cggtgcacgc
     1021 cggtggtggt ttcttcggtc acgcccttga tgtccttgcc gaccttcgtg taccagccgg
     1081 ggctggcggc gagcttcttc ctgatcgcgt tgaacagcga ggtctcctcc tcgctcgagg
     1141 ggctggccag caccgaggga tcccgctccg ccttctgccc gaggtgcacc agcagcgtgg
     1201 cgtcgccgcc gtcgtccagg atcatgttcg gccccttgtc gccgaactcg aagatgcggt
     1261 gggtgtagtc ccagtactcg tcgagcgtct cgcccttgac ggcgaacacc ggggtgccgc
     1321 cggcggcgat cgccgccgcg gcgtggtcct gggtggaaaa gatgttgcag gaggcccagc
     1381 gcacttcggc gccgagcgcc ttcagcgtct cgatcagcac cgcggtctgg atggtcatgt
     1441 gcagcgaccc ggtgatgcgc gcgcccttca gcggccgcga cctggcgtac tcgtcgcgga
     1501 tcgccatcag cgccggcatc tccgattcgg cgatggcgat ttccttcctg ccccagccgg
     1561 cgagcttgag gtcggcgacg cggaagtcgc cggacttggc gttcatgacg gcagcattca
     1621 tggttccagc ctccagaaag caaaagcgcc cgcaccgttt ccgatgcgag cgccgttgaa
     1681 tttagctgag cctgaccccc tcggatggcc gtcggggacg caacgctcct cagcaagcgc
     1741 gattctagtc gcattgccct ccttttaaaa gacctgcaaa ccctgcatgc ttggcggcca
     1801 tgccggccgc ccagccctcc gtcttcgcca gcgtcgcgtt cctgaagatc cacgacttcg
     1861 gccgccggcc ggtggtcgag cagacgcgcc tgcgcgccca gctcgaggcc gcggtggcgg
     1921 tgaccctgca cgcgctggcg gaaccgagcc gcgtggtgct ggatgccgcc gacggcgtcg
     1981 ccctggtggt gttcggcgat ccgcgcggag cgctgcgcat cgccgagcgc gcgctctccg
     2041 ccggcgccgc gggactgccg ctcgccatcg gcatcacgca cggcgcggtg cgccagaccg
     2101 gcgagggcgc cggcgaaggc ctgctgggcg acggcatcgc ggtggcggcg gcgatcgccg
     2161 agctcgccgg gccgcagaag ctgctgctga cgcgcgaatt ccgcgccgcc ctcgccgacg
     2221 ccgcgccggg cgccgaggcg gcggtggcgg gcgccggcac ccacaccgac gccagcctgc
     2281 gctcgcacga gctctaccgg cccgatgcgt tcgcggcgcg ccgccgtgcg cgccgctacg
     2341 ccgccgcgac ggtgctcgcc gcgctctgcc tggtcggctc gggcgctggc taccgcgcct
     2401 cgatcgaagg accgcgcgcc tacgccgggc gcgtggccgc cgacctgaag ggcgctgcgg
     2461 ccaaggcgca ggagcgcgcg ctgcggctcc tgcagcagac gggccgctga tggcgaaaaa
     2521 acgcaaggag aaccagatcg ggcgctaccg catcctcggc gagctcggcc gcggggcgat
     2581 gggcgtggtg taccgggccg aggacccgtc gctcgatcgc gtggtggcgc tgaagaccat
     2641 ttcgctcgcc aacgccgacg aagaccgcaa gtcctacgag aagcgcttcc tgctcgaggc
     2701 gaaggccgcg ggcaagctca cccacccgaa catcgtcacc atcttcgact acggcgagga
     2761 ggaggacctc gcctacatgg cgatggagct gctcgagggc gccgacctgc gcacgcgcat
     2821 gcgccagggc gagatcgcgc cgctggaggc ggtggagatc gcgctgcagg tggccgacgg
     2881 cctgggcttc gcgcacgaga ccggggtcgt gcaccgcgac atcaagccgg gcaacatcat
     2941 gctgctggag cgcggcgccg tgaagatcat ggacttcggc atcgcgcgca tgcgccacgc
     3001 cgaccacaag accagcaccg gcatggtgct cggcacgccg cgctacatgt cccccgagca
     3061 gatcgccggg cagccggtgg accagcgctc ggacatcttc tcgctcggca ccgtgctcta
     3121 cgagatgctc acgcgcacca tgctgttcgc cggcgacgac gtcaaccaga ttgcgcacaa
     3181 cctcgccaac gccgagccgg tgccgccgtc gcgcagcaat ccggcgatcc cgcagatcgt
     3241 cgacttcatc accgcgcgcg ccctgaagaa ggacccggcg gtgcgctacc aggacgccta
     3301 cgaaatggcg gccgatctgc gcgacgcgct cgcggagatg cgcggccgcg cgccggcgcc
     3361 gcgcaacggc gatgccgagg ccacgcacac catgaagctc gagccgggcg cgcacaagcc
     3421 gccgctcgcg cccgcggcga gcgccatcgc gcgcgacacg cggctgccgc tgtcgcgcct
     3481 gttcgacagc gaggcggcgc tcgagcggct ggccgtgccc aagcacagcg cgcgcctggc
     3541 gcgcgcgccg cggcaggtgg ggctgttgcg gcggatcttg cgcgaccggc cggtgcggcg
     3601 gctgtgcgcc gtggcgctcg tcgccgccct gctcggcagc tggatcgcga tcggctgagt
     3661 gcggcgcgcc tgcactatcg tgccgccggc aggcggcttg tcagtgtcgg accttcgcgc
     3721 caagatgggc actcatctct gggaggagat tcgccatgca gaagatcgtg cccggccgcc
     3781 ggcccttcat cgtccgcgca ctcggggcgc tcgcgtcgct gccgctgctc caggcctgca
     3841 tgggcggcgc tggcggacag gcgcgctccg cgccggcggc tcccggaagc tttgatttcg
     3901 gcgtcgtcgg cgacatcccc tacaccaggg cgcaggaagc cgagtacctg cgggtcatgc
     3961 aggacatcaa ttccagggat ctcgaattcg tcgcccacat cggcgacacc atgttcgacc
     4021 cgcggcccta cgagagggat ccgtcgctcg ctcgcacgcc cctgaccgac gaaaactacg
     4081 cctatgtgct cgggaccttc cagagttgtc ggcatccgct ggtgttgacg ccgggcgaca
     4141 acgactggtc ggacgcggtg cagttccaga aagtgaaggt cgacccgctc gagcgtctgg
     4201 cgaagctgcg cgccaccttc tacccgaagg gccgcagcct cggccagcgc acgatggccg
     4261 tggaaagcca ggcgcaggat ccggtgtacg cgacctacgt ggagaacctc acctgggccg
     4321 tgcgggggct gcggttcgcg accctgcaca tcgtgggcag caacgacaat gcgcgcacct
     4381 cgatggacga gcacaaagcg cgtcaggccg cggtcatcgc gtggatgcgc aaggccttcg
     4441 cggcagccaa ggcgggcggc agcctgggcc tggtgctgat cacgcatgcc aatccggatt
     4501 tcgagaaccg ctggacgcgc tcctacgcct cgcgctacgc gaggagcgtg cgcggcgccg
     4561 aagcgcccaa ggcgcccgag gccacgccct acgacccgtt cctcgacgcc ctgatcgagc
     4621 agatgcaggg ctacgacaag ccggtgctgt acgtgcacgg cgacacgcac atcttccgca
     4681 tcgggaatcc gctgatgaat ccgaagacgc agcgcttctt cgagaacctg acgcggctgg
     4741 agacctttgg ctggcccgac tcggcctggg taagggtgac ggcgaacccg tccaaccccc
     4801 acctgttcga gatccacccc gagtatgtcc ggggcaattc ggcgaactac aaagcctagg
     4861 cggtcaggct gcctgtttga gcgccaggac cttgtcggtc cgctcccagg tgaattcggg
     4921 ctcctcgcgg ccgaagtggc cgtaggcggc ggtcttctcg tagatcgggc gcaggaggtc
     4981 cagcatctgg atgatgccct tgggccgcag gtcgaagttg gcgagcacca gctgcgagag
     5041 tttctcgtcg gcgatcttgc cggtgccctg ggtggtgacc atcacgctgg tgggctgcgc
     5101 cacgccgatg gcgtaggaca cctgcaccag gcacttcgag gcgagccccg cggcgacgat
     5161 gttcttcgcc acgtagcgcg ccgcgtaggc ggccgagcgg tccaccttcg agggatcctt
     5221 gccggagaag gcgccgccgc cgtgcggcgc tgagccgccg taggtgtcga cgatgatctt
     5281 gcggccggtg aggccgcagt cgcccttcgg gccgccgatc tcgaagctgc cggtggggtt
     5341 gacgaggtac ttgaccttgc ccttcagcag gttcttcggc agcaccggct tgatcacctg
     5401 ctcgatcacc gcctcctcga tctgcttgtg ggtcatgccc ggcttgtgct gcgtcgagag
     5461 caccacggtg tctatcgaat cgggcttgcc gttggcgtag cggatggtga cctgggactt
     5521 cgcgtccggc cgcagccagg cgaggcgccc gtcgcggcgc agctcggact ggcgctcgac
     5581 caggcggtgc gacaggtaga tggccatcgg catcaggctc ggcgtctcgt cgcaggcgta
     5641 gccgaacatc aggccctggt cgccggcgcc ctggtcgagg tcgagcccct tgccttcatc
     5701 gacgccctgg gcgatgtcct tggactgctc gccgtaggcc acgatcaccg tgcaggtgtg
     5761 cgtgtcgaag ccgatcaccg ggtcgtcgta gccgatgcgc ttgacggtcc tgcgcaccac
     5821 gctctggtaa tccaccttgg cgccggtggt gatctcgccg gccagcacca ccaggttgtc
     5881 cttcaccagc gtctcggcgg cgacgcgcga ggtcttgtcc tggcttagaa tcgcgtccag
     5941 caccgcgtcg gaaatctggt cggcgacctt gtccggatgg ccctcggaca ccgactcgga
     6001 agtgaaaaga aattcgctca tggggctcgc ctgaaaaaag ggttgaagga tagcagatga
     6061 tgcagctggc ggcgcgcctc ccgctgcgcg tcctgcacgc gttgggcagc ctcctcgggt
     6121 gggcgatgta cgcgctgtcg cccacctacc ggcgccgcct gcgcgccaac ctcgagcagg
     6181 ccggctacac cgatgcgcgc acgcgccgcg aagcgatcgc cgaggcgggc aagctgatca
     6241 ccgagtcgcc caagctctgg ctgcgcccgc gcgaagaggt cctcaagctg gtgcacggct
     6301 tcgaagggct ggaggaggcg gcgcgcgtgc acgccgcggg ccgcggcatc gtgttcctga
     6361 cgccgcacct ggggtgcttc gagatcaccg cgcaggcggc ggccgagcgc gtgcccctca
     6421 ccgtgctcta ccgcccgccc aaggtcgact ggctgcgccc gttgatggag cggggcaggg
     6481 gcggccggaa catgaccctg gcgagcgcgg acctcggcgg cgtgcggcaa ctgctctccg
     6541 cgctcaagcg acgcgagacg gtgggcatcc tccccgacca ggtgcccggc gagggcgagg
     6601 gcgagtgggc cgaattcttc ggccggccgg cctacaccat gacgctcgcc gcgaagctca
     6661 tcgcgcgcgg cgacgtggcc tgcttcctcg ccgtcgggcg gcggctgccg cgcggcgcgg
     6721 gctacgccct gcgcatggcg ccgctgccga gcgccctgcc cggcgaaagc gggacgcggc
     6781 gcatcaaccg cgccctcgaa gcggcgatcc gcgagtgccc cggccagtac ctgtggggct
     6841 acaaccgcta caagaagccg cggggcgggc gatgagccgc gtggtgttct tcctcatgtg
     6901 gctggtccac ttcctgccgc tccggatcca ggcggcgatc ggcaatgcgg cgggcgcgct
     6961 cctctactgg ctgatcccgg agcgccggcg cgtgacgcgc atcaacctcg ccaagtgctt
     7021 cccgcagatg gacccggcgg cgcgcgagcg cctggcgcgc gcgcacttcc gctcgttctg
     7081 ccgcggcttc gtcgagcatg gcctgctgtg gtgggcgccg cgcgagagga tcgagcgcct
     7141 ggtgcgcgtc gagggcctgg agcacctgcg cgcgctcggc gccgcgccgg cgctgctgtt
     7201 cgtgccgcac ttcgccggcc tggatgcggg tttcacgcgg ctgtgcatgg agctggacat
     7261 ggtgggcatc tactcgcggc agaaggacgc gcgcttcggg cggctgctgg cgctgggccg
     7321 cgggcgcttc ggcaaccagc gccaggtgtc gcggcaggaa ggggtgcgcg ccgcgatcac
     7381 cgccatgctg cgcgagcacc gggccttcta ctacctgccc gaccaggacc acggtccgct
     7441 gaacgcggtg ttcgtgccgt tcttcggcgt gcccgcggcg accgtgccgg gcctgtcgcg
     7501 catcgccaag ctctcgggcg cccgggtgct gccctgcgtg acgcgcatgc tgcccggcgg
     7561 acagggctac ctggtgcgca tcgagccgcc ctgggagggc tttcccaccg cggacctgct
     7621 ggccgacacg cggcgcatga acgcccacat cgaagcggtg tgccgcgaga tcccggagca
     7681 gtacctgtgg atgcacaagc gcttcaagac gcgccccgag ggcgaggcgc gcttctactg
     7741 actagaatgc agccatgaaa aagacgctgc gcgccgccgt gatcggcgtc ggctacctcg
     7801 gccagttcca cgccgagaag ctggcggcgc tgcagggcgt cgagctggtg gcggtggtcg
     7861 acgcggatgc cgcgcgcgcg aagcaggtcg ccgcgaagca cgcctgcgcg gcgctcgccg
     7921 acccgggcgc cctggccggg cgcgtggacc tggtgagcat cgcggtgccc accgaccggc
     7981 accacgccgt ggcgctgccg ctgctcgagg cgggagtgca cgcgctggtc gagaagccca
     8041 tcgcgcgctc gctcgaggag gccgacgcga tgctcgccgc ggcgcgccgc ggcggcgcgc
     8101 tgctcgcggt cgggcacctg gagcgcttca atcccgcgtt ccgcgccctc gccgccgcgt
     8161 cgacgaagcc cctgttcatc gattgcgagc ggctctcggc cttcaagcag cgcggcatcg
     8221 aggtggacgt ggtcctcgac ctgatgatcc acgacctgga cctggtgctg tcgctcgcca
     8281 ggggcgagat cgcgcagatc tccgcctgcg gcttccaggt gctcaccgat tcgatcgaca
     8341 tcgccaacgt gcgcatcgag ttcgccgacg gctgcgtcgc caacctgtcg gccagccgcg
     8401 tcagccagtc gccggtgcgg aagctgcgcg tgttccaggg cgacggctac gcctcggccg
     8461 acctgcaggc gggcaagctg cgcatggtgc gtcgcgacgc gcgcaaaggc gggatcgtcg
     8521 aggacgaggc cacgttcgac gaccgcgatg cgctgcgcgc ggagatcgag ggcttcgtcg
     8581 acgcggtgcg cggcggccgc cggccgccgg tggcgggcga ggacggccgc aaggcgctgg
     8641 cgctggcgct ggaagtcggg cgcctggtgc gcgcgcggct ggagcgcttc aaggcggtcg
     8701 cggcgtgaag gtcccgatcc tcgacctggc cgccgagctg cgcgaggtgg gggcggaggt
     8761 gcgcgcggcc ctggagcgcg tgctcgcctc cggccacttc atcctcggcc cggagggcgc
     8821 ggcgctggaa cgggagatcg cggcgctgct gggcgtgccg cacgcggtgg cctgcaactc
     8881 gggcaccgac gcgctgcacc tcgcgctgcg cgccgcgggc atcggcccgg gcgacgaggt
     8941 ggtgacgccg gcctttacgt tcattgccac cgccgaggcc atcgcgtacg tgcacgcgcg
     9001 accggcgttc gccgacgtgg accccgcgac cttcaacatg accgcggcga cgctggagag
     9061 ggcgctcacg ccgcgcacgc gcgcggcgat cgtggtgcac ctgttcggcc agtgcgccga
     9121 gatgccgccg atcgccgagc tgtgccgcaa gaagggactg gtgctgatcg aggactgcgc
     9181 gcagtccatc ggcgccgact gggacggccg gcagtcgggc gcctggggcg atttcggctg
     9241 cttttccttc tacccgacga agaacctcgg cgcctacggc gacgccggcc ttgtgaccgc
     9301 gcgcacggcg cagcacgacg cggcgctgcg catgctgcgc caccacggca gccgggtgac
     9361 ctactcgcac gaggtgatcg gcctgaacag ccggctcgac gagatgcagg cggcgatcct
     9421 gcgcgtgaag ctcaagcacc tggcgcgctt cagcgcggcg cggcgcgcgc atgcggcgcg
     9481 ctaccgcgag ctgctggcgg gcgccgcgct gcggctgccc gccgagcacg ggcacggtac
     9541 gcacatctac caccagttca cgctgcgcct ggcgcgccgc gacgcggcca agcaggcgct
     9601 cgccgaggcc ggcgtcgcga gcggcgtgta ttacccgatc ccgctgcacc gccagccggt
     9661 gttcgcgaag gagttcggcg cgctgtcgct gccggccgcg gaagccgcgg cgcgcgaagt
     9721 gctgtcgctg cccatctatc cgcagctcgc cgacgagcag ctgcgccacg tcgccgccag
     9781 cctgcgcgct tgcctctagt cgccttcacc aagatggagg gcgcgggcaa cgacttcgtc
     9841 gtgctcgacg cgaccgcgaa gccgttcgcg ctctccggcg agcagctgcg gcgccttgcc
     9901 gaccggcatt tcggcgtcgg ctgcgaccag gtgctggtgg tggagaaggc gcggcaaccc
//
LOCUS       DASRIE010000161         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2781629, whole genome shotgun sequence.
ACCESSION   DASRIE010000161 DASRIE010000000
VERSION     DASRIE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781629"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFB53_07015"
     CDS             complement(<1..330)
                     /locus_tag="VFB53_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86520.1"
                     /translation="MRGFGLLAWLLISGCAATAPAAPDSGVAVVWNRVEDPHTTCEAL
                     SGRKSFFNVLGCSQWQQPVAQGAPRVCAIYAPAPRNERDMQRFATLGHELMHCFDGNW
                     HDRWGRMN"
     gene            complement(327..1007)
                     /locus_tag="VFB53_07020"
     CDS             complement(327..1007)
                     /locus_tag="VFB53_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZN86521.1"
                     /translation="MNAPLAAKAFQARSISLASRYGGRTVLAIGAHPDDLELAIGGML
                     ARLSRESARVVMAVVSIPADFETRRAEAERAAEILGCELRILMDDEAKRIDDIKNYQL
                     VGMLDALVRELQPAAVLTHSANEFHRDHVTVHNACISTQRLKPFDFFHFNPTMCRPVP
                     VQFYPRAYVDVTSTLDLKMEAIAAHVSQFGARGLEAEMYRDLARLTGRMVGVKYAEGI
                     DVSRMLLA"
     gene            complement(1004..1738)
                     /locus_tag="VFB53_07025"
     CDS             complement(1004..1738)
                     /locus_tag="VFB53_07025"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HZN86522.1"
                     /translation="MIIAIHQPHYLPWLGYLHRMAQVDAFVLLDHVQFERRNYQNRTM
                     VRMNGEARWLTVPVIQHSQKERIVDKQVDNRLQGPKSWSSVHFATLRHAYGASGFFAG
                     YAAQFKQLFETRFDRLVDLNQAGLDLLRAAFAIRTPLVRSSELAVEGARGDLILNICR
                     ALGADTLLVGFGGSRGYLDTDAFERAGVGIAQHQFTHPEYAQGETKAGEAAPFLRGLS
                     AVDMLFNCGPQSRERLLAPRALAKAA"
     gene            complement(1792..>2128)
                     /locus_tag="VFB53_07030"
     CDS             complement(1792..>2128)
                     /locus_tag="VFB53_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86523.1"
                     /translation="ETYEKCSPGILAEVDNVRQFMEAPAPRWIDSNTSRDNTSYGRVW
                     KDRITVQRVAVGTGGLARIAVAALPMLRLAAQWRRSLAGRVGLQRRRAPATAVASPRP
                     ATRDVRQAA"
BASE COUNT          324 a          723 c          770 g          311 t
ORIGIN      
        1 attcatccgg ccccagcggt cgtgccagtt gccgtcgaag caatgcatca gctcgtggcc
       61 gagcgtggcg aagcgctgca tgtcgcgctc gttgcgcggc gccggcgcgt agatcgcgca
      121 cacgcgcggc gcgccctgcg cgaccggctg ctgccattgc gagcagccga gcacgttgaa
      181 gaagctcttg cggccgctca gcgcctcgca ggtggtgtgc ggatcctcga cccggttcca
      241 caccacggcc acgccggaat cgggcgctgc gggcgcggtg gcggcgcagc ccgagatcag
      301 caaccaggcg agcagaccga aacccctcat gccagcagca tgcggctgac gtcgatgccc
      361 tcggcatact tcacgcccac catgcggccg gtgagccgcg ccaggtcgcg gtacatctcg
      421 gcttccaggc cgcgcgcgcc gaactggctg acgtgcgccg cgatcgcctc catcttcagg
      481 tcgagcgtgc tggtgacgtc gacataggcg cgcgggtaga actgcaccgg caccgggcgg
      541 cacatggtcg ggttgaagtg gaagaaatcg aacggcttga ggcgctgcgt ggagatgcac
      601 gcgttgtgca ccgtcacgtg gtcgcggtgg aactcgttgg cgctgtgcgt gagcaccgcg
      661 gccggctgca gctcgcgcac cagcgcgtcg agcatgccga ccagctggta gttcttgatg
      721 tcgtcgatgc gcttggcctc gtcgtccatc aggatgcgca gctcgcagcc gaggatctcg
      781 gcggcgcgct cggcctcggc gcggcgggtc tcgaagtcgg cgggaatcga gaccaccgcc
      841 atcaccacgc gcgccgactc gcgcgacagc cgcgccagca tgccgccgat cgcgagctcg
      901 aggtcgtcgg gatgcgcgcc gatggcgagc acggtgcgcc cgccgtagcg cgaggcgagc
      961 gagatgctgc gcgcctggaa cgctttcgcc gcgagcgggg cgttcatgcg gccttggcca
     1021 gcgcgcgcgg cgcgagcagc cgctcgcggc tctgcggacc gcagttgaac agcatgtcca
     1081 ccgccgacag gccgcgcagg aacggcgccg cttctccggc cttggtctcg ccctgcgcgt
     1141 actcggggtg cgtgaactgg tgctgcgcga tgccgacgcc ggcgcgctcg aacgcgtcgg
     1201 tgtcgaggta gccgcgcgag ccgccgaagc cgaccagcag cgtatcggcg ccgagcgcgc
     1261 ggcagatgtt gaggatcagg tcgccgcgcg cgccctcgac cgcgagctcg gagcttcgga
     1321 cgagcggcgt gcggatggcg aaggccgcgc gcagcaggtc gagcccggcc tggttgagat
     1381 ccaccaggcg atcgaagcgc gtctcgaaca gctgcttgaa ttgcgcggcg tagccggcga
     1441 agaagccgga cgcgccgtag gcatggcgca gcgtggcgaa gtgcaccgac gaccacgact
     1501 tcgggccctg gagccggttg tccacctgct tgtcgacgat gcgctccttc tgcgagtgct
     1561 ggatcaccgg caccgtgagc cagcgcgcct cgccgttcat gcggaccatg gtgcggttct
     1621 ggtaattgcg gcgctcgaac tggacgtggt cgagcagcac gaacgcgtcg acctgcgcca
     1681 tgcgatgcaa gtagccgagc cacggcaggt aatgcggctg atggatcgcg atgatcatga
     1741 gagtgccggg cagcgtaccg aggagcggct cgactatgga accggcgcga cctaagccgc
     1801 ttggcgcacg tcgcgggtgg cgggacgcgg cgaagcgacc gcggtggccg gagcgcgacg
     1861 tcgctgcagc ccgacgcgcc ccgccagcga gcgccgccat tgcgcggcca gcctcagcat
     1921 cggcagcgcg gccaccgcga tgcgcgccag cccgccggta ccgaccgcga cgcgctgcac
     1981 ggtgatacgg tccttccaca cccggccgta gctggtgttg tcgcgcgaag tattggagtc
     2041 gatccagcgc ggcgccggcg cctccatgaa ctggcgcacg ttgtcgacct cggccaggat
     2101 gccgggcgag cacttctcgt aggtctcg
//
LOCUS       DASRIE010000162         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3832692, whole genome shotgun sequence.
ACCESSION   DASRIE010000162 DASRIE010000000
VERSION     DASRIE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832692"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..586
                     /locus_tag="VFB53_07035"
     CDS             2..586
                     /locus_tag="VFB53_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZN86524.1"
                     /translation="MLQSRIAAHFAESAQLKLAAAETMAAPVARAAELFVEVLRGGGK
                     ILSCGNGGSAADAQHFAAELLNRFEIERAPLAAIALAMDSSTMTSIGNDYSYQQVFSK
                     PLRALGRKGDALFAISTSGNSGNVIEAIKVAHEFGIRVVALTGNGGGKIAGLLRAEDV
                     HLCVPHKRTIRIQEVHLLCLHCLCDAIDTLLFPR"
     gene            550..1191
                     /locus_tag="VFB53_07040"
     CDS             550..1191
                     /locus_tag="VFB53_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN86525.1"
                     /translation="MRRHRYLAVPALIFVLGGALPGLQGCAAIIGVGGIAAVSTLEDR
                     RSTGAQFDDNTIESRAASRISERFGERAHVNVAAFNRAVLLTGETWDEATRAEIEKIV
                     AEVPNVRTVSNEIQVGGLSSTTSRANDAALTARVKGRFLNSREFSSLHVKVVTEAGVV
                     YLLGLVTESEAAAATELARTTSGVRKVVRVFDYCKDRDELCRPGPSSKPKPAA"
     gene            1215..1670
                     /locus_tag="VFB53_07045"
     CDS             1215..1670
                     /locus_tag="VFB53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZN86526.1"
                     /translation="MRDPAALGRWISGLARPLVFTNGVFDLLHRGHVTYLARARALGA
                     ALLVGLNTDHSARRLGKGADRPLSTLADRAALLAALECVDAVTWFDEDTPEALIAACR
                     PQVLAKGGDWPAERIAGAGGVLARGGQVVSIPFEHQLSTTRLIERIRKG"
     gene            complement(1667..>2018)
                     /locus_tag="VFB53_07050"
     CDS             complement(1667..>2018)
                     /locus_tag="VFB53_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN86527.1"
                     /translation="CCFVDFERELASLPGEYRLFFADEVAGCVAVRLLNPETAEMKRL
                     YVRSAHRGKGLGRALAEAAIAAAREAGCGRIVLDTLPKMRAAQALYRALRFKEISPYL
                     EHPTPGALCFELKL"
BASE COUNT          332 a          688 c          704 g          294 t
ORIGIN      
        1 ggtgcttcag tcccgtatcg cggcgcattt cgccgaaagc gcgcagctca agctcgccgc
       61 cgccgagacg atggccgcac cggtcgcgcg cgcagccgag ctgttcgtgg aagtgctgcg
      121 cggtggcgga aagatcctct cctgcggtaa cggcggctcg gccgccgacg cgcagcactt
      181 tgccgccgaa ctccttaacc gcttcgagat cgagcgggcg ccgcttgccg cgatcgcgct
      241 cgcgatggac agctcgacca tgacctcgat cggcaacgac tacagctacc agcaggtatt
      301 ctccaagccg ttacgcgcgc ttggccggaa gggcgacgcg ctgttcgcga tctcgaccag
      361 cggcaactcg ggcaatgtga tcgaggcgat caaggtcgcg cacgagttcg ggattcgcgt
      421 ggtcgcgctc accggaaacg gcggcggcaa gatcgcaggc ctgctgcgcg ccgaggacgt
      481 ccacctgtgc gtgccgcaca agcgcaccat ccggatccag gaagtccacc tgctgtgcct
      541 tcactgccta tgcgacgcca tcgatacctt gctgttcccg cgctgatttt cgtcctcggc
      601 ggcgcgctgc ccggcctgca gggctgcgcg gcgatcatcg gcgtgggcgg catcgcggcc
      661 gtcagcacgc tcgaggaccg gcgcagcacc ggcgcccagt tcgacgacaa cacgatcgaa
      721 tcgcgcgcgg caagccgcat cagcgagcgc ttcggcgagc gcgcccatgt gaatgtcgcc
      781 gcgttcaacc gtgcggtgct gctcacgggt gaaacatggg acgaggcgac ccgcgccgag
      841 atcgagaaaa tcgtggccga ggtaccgaac gtccgcaccg tcagcaacga gatccaggtg
      901 gggggcctgt cgtcgaccac gtccagggcg aacgacgccg cgctcacggc gcgggtcaag
      961 ggccggttcc tgaactccag ggagttcagc tcgctgcacg tcaaggtggt cactgaagcg
     1021 ggcgtggtct acctgctcgg cctggtcacc gaaagcgagg ccgcggcggc gaccgaactc
     1081 gcgcgcacca ccagcggcgt gcgcaaggtg gtcagggtgt tcgactactg caaggaccgc
     1141 gacgagcttt gccgcccggg accctccagc aagcccaagc ccgcggcgtg aagccgcctg
     1201 aattcgaggc gaagatccgc gacccggcgg cgctggggcg ctggatcagc ggcctggcgc
     1261 ggcccctggt gttcaccaac ggcgtgttcg acctcctgca ccgcgggcat gtgacgtacc
     1321 tcgcgcgggc gcgcgcgctc ggcgcggcgc tcctggtcgg gctgaacacc gatcactccg
     1381 caaggcggct gggcaagggg gcggatcgcc ctctcagcac gcttgccgac cgtgcagcgc
     1441 tgctggcggc gctcgagtgc gtcgatgcgg tgacctggtt cgacgaggac acgccggaag
     1501 cgctgatcgc cgcctgccgc ccgcaggtgc tcgcgaaagg cggcgactgg ccggccgaac
     1561 gcatcgccgg ggcgggcggc gtgctcgcgc gcggcggcca ggtggtgtcg attcctttcg
     1621 agcaccagct ttccaccacg cgcctgatcg aacggatccg gaaaggctag agcttcagct
     1681 cgaagcagag cgcgccgggc gtgggatgtt cgaggtaagg agaaatctcc ttgaaacgca
     1741 gggcgcggta gagcgcctgg gccgcgcgca tcttcggcag cgtgtcgagc acgatgcgcc
     1801 cgcagccggc ctcgcgcgcc gcggcgatcg cggcttcggc gagcgcgcgc cccaggccct
     1861 tgccgcggtg cgcgctgcgc acgtagagcc gcttcatttc ggcggtttca ggattcagca
     1921 agcgcaccgc aacgcaaccg gcgacctcgt cggcgaaaaa cagccggtac tcgccgggca
     1981 gggaagcgag ctcacgctcg aagtcgacga agcagcag
//
LOCUS       DASRIE010000163         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3560099, whole genome shotgun sequence.
ACCESSION   DASRIE010000163 DASRIE010000000
VERSION     DASRIE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3560099"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFB53_07055"
     CDS             <1..256
                     /locus_tag="VFB53_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN86528.1"
                     /translation="GLLPFRTGAFQVAAQAGIPVVPIALRGVRSVLRDGTWFPRRYPI
                     AVTIGAPITPEGNDWNAAVKLRDRVRAEVLKFCGEADAAG"
     gene            complement(253..2091)
                     /locus_tag="VFB53_07060"
     CDS             complement(253..2091)
                     /locus_tag="VFB53_07060"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86529.1"
                     /translation="MLKRLFGAKAAAPDSAEACFRLGTDLAQRGQLDQAIVLLRRATE
                     LAPHGAGAYHNLASAYRDRGDLQAALAAYRDAAQLDPNFADAHYGVGLMLMVERRYEE
                     ALASLRRAIKLDPRLAEARFQAGNAHMGLGDWQAALAEFKAAIDARPDYAEARWARAM
                     SQLPAVYGAGTDPVSRRHAFTAQLEALRKWFHTTRRPDAHLAVGVHQPFYLAYQEAPN
                     RELLGKYGALCTELMGAWQSGAGLALPAGRREAGKTRGKPRIGIVSAHIHDHSVWTAL
                     VRGWVERLSAANELHLFHLGTTQDAETKFARRRAAGFLDGIRPFDDWARAIHGAQLDL
                     LLYPEIGMDATSAKLAALRLAPAQAASWGHPETSGLPTIDYYLSAQALEPADAQENYT
                     EKLVALPNLGCWLVPGEEASAKPPVLEVAADEVLLLCPGNAFKYAPQQDRVLTALAQR
                     VPQARLVFFRSRPDALAERLRERLRESFRRAGVDFERRVIFLPWQSRASFRALLVRAD
                     LYLDTIGFSGFNTAVHALECGLPIVAREGRFLRGRLASGMLRHLGLGELVAESDEAYV
                     ELAAALAADRERRQALRRRIPSARQRLYRDAAPLDALQRFVESVVG"
     gene            complement(2085..>2502)
                     /locus_tag="VFB53_07065"
     CDS             complement(2085..>2502)
                     /locus_tag="VFB53_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZN86530.1"
                     /translation="PLKPATPVVKVDVGPVWVIGEMTDAAALAALKPDMAALAEWSLS
                     LGVSGATVFAPAGESASAIHVRSFAPAHAIPEDPVCGTGNLSVAAFVEKRFGPEYVAR
                     QGMQMGRDGRVFVRVGPQGVYLGGHAVTCVDGTLAC"
BASE COUNT          362 a          887 c          914 g          339 t
ORIGIN      
        1 ggggctgctg ccgtttcgca ccggcgcgtt ccaggtcgcg gcgcaggccg gcatcccggt
       61 ggtgccgatc gcgctgcgcg gcgtgcgctc ggtgctgcgc gacggcacct ggttcccgcg
      121 ccgctacccg atcgcggtca ctatcggtgc cccgatcacg cccgaaggca acgactggaa
      181 tgctgcggtg aaattgcgcg accgggtgcg cgcggaagtg ctgaagttct gcggcgaggc
      241 cgacgccgcg ggctagccga cgacgctttc gacgaagcgc tgcagcgcgt cgagcggcgc
      301 ggcatcgcgg tacaggcgct ggcgggcgga cgggatgcgg cggcgcaggg cctggcggcg
      361 ctcgcgatcg gcggcaagcg cggcggcaag ctcgacgtag gcttcgtccg attccgccac
      421 cagctcgccg aggccgagat ggcgcagcat gccgctcgcc agccgcccgc gcaggaaacg
      481 gccttcgcgc gccacgatcg gcaggccgca ttcgagcgcg tgcaccgcgg tgttgaagcc
      541 ggagaagccg atcgtgtcca ggtagagatc ggcgcgcacg agcagcgcgc ggaacgacgc
      601 ccggctctgc cacggcagga aaattacccg ccgctcgaag tcgacgccgg cgcggcggaa
      661 cgactcgcgc aaccgctcgc gcagcctttc cgccagcgca tcggggcggc tgcggaagaa
      721 caccagccgc gcctgcggca cgcgttgcgc gagcgcggtc agcacgcggt cctgctgcgg
      781 cgcgtacttg aatgcgttgc ccgggcagag cagcagcacc tcgtcggcgg ccacctccag
      841 caccggcggc ttggcggacg cctcctcgcc cgggaccagc cagcagccga ggttcggcag
      901 cgccaccagc ttttccgtgt agttctcctg cgcatcggcg ggctccagcg cctgcgccga
      961 aaggtaatag tcgatcgtgg gcaggccgct ggtttcggga tggccccagc tcgccgcctg
     1021 cgccggcgcg aggcgcagcg cagcgagctt cgcgctggtc gcgtccatgc cgatctcagg
     1081 gtagagaagg agatcgagct gcgcgccgtg gatggcgcgc gcccagtcgt cgaaaggccg
     1141 gatgccatcg aggaagccgg ccgcacggcg gcgggcgaac ttggtctccg cgtcctgtgt
     1201 cgtgccgagg tggaagagat gcagctcgtt ggccgcggag agccgctcga cccagccgcg
     1261 caccagcgcg gtccacaccg aatgatcgtg gatgtgcgcc gagacgatgc cgatgcgcgg
     1321 cttgccccgc gtcttacctg cctcgcgccg cccggccggg agcgcgagcc cggcacccga
     1381 ctgccacgcg cccatcagct ccgtgcacaa ggcaccgtac ttcccgagca gctcgcggtt
     1441 cggcgcctcc tggtaggcaa gatagaacgg ctgatgcacc ccgaccgcca gatgggcgtc
     1501 cggccggcgc gtggtgtgaa accacttgcg cagcgcctcg agctgcgctg tgaacgcatg
     1561 gcggcgcgag acggggtcgg tgccggcgcc gtacacggcc ggcagctgcg acatggcgcg
     1621 cgcccagcgc gcctcggcat agtcggggcg cgcgtcgatc gccgccttga actcggcgag
     1681 cgccgcctgc cagtcgccga ggcccatgtg ggcattgccc gcctggaagc gcgcttctgc
     1741 gagccgggga tccagcttga tcgcgcggcg caggctggcg agcgcctctt catagcggcg
     1801 ctcgaccatc agcatcagtc cgacgccgta gtgggcgtcg gcgaagttcg ggtcgagctg
     1861 cgccgcgtcg cggtaggcgg ccagcgccgc ctggaggtcg ccgcggtcgc ggtaggcgct
     1921 cgcgaggttg tggtacgcgc cagcgccgtg cggcgcgagc tcggtcgccc ggcgcagcag
     1981 cacgatcgcc tggtcgagct gcccgcgctg cgcgaggtcg gttccgaggc gaaagcaggc
     2041 ctcggccgaa tccggcgccg cagccttggc gccgaacaat cgcttcagca tgcgagcgtg
     2101 ccgtccacgc aggtgacggc atggccgccc aggtagacgc cctgcggccc tacgcgcacg
     2161 aaaacgcggc cgtcgcgtcc catctgcatc ccctgccgcg cgacgtattc gggaccgaag
     2221 cgcttctcga cgaacgcggc aacgctgagg ttgccggtgc cgcaaaccgg gtcttccgga
     2281 atggcgtggg ccggtgcgaa cgagcgcaca tggatcgcgc tcgcggactc gccggccggc
     2341 gcgaacacgg tggcgccgct gacgccgagc gacaggctcc actccgcgag cgcggccatg
     2401 tcgggcttca gcgcggccag cgcggctgcg tcggtcatct cgccgatcac ccacaccgga
     2461 ccgacgtcga ccttcaccac cggagtggct ggcttgagcg ga
//
LOCUS       DASRIE010000164        10926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_171119, whole genome shotgun sequence.
ACCESSION   DASRIE010000164 DASRIE010000000
VERSION     DASRIE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171119"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFB53_07070"
     CDS             <1..734
                     /locus_tag="VFB53_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN86531.1"
                     /translation="VSGKKIFASLSGAADYYGILCTERAEGEQASRRNTLYLAVPANA
                     PGVSVVGDWDPLGMRGTVSRTLLMKDVFVPESELLMPHGVYFRAATSWPHMFLTLSPT
                     YMGLAQAAYDFTVAYLRGEAPGTPPVKRRMFPTKQIAVAEMWVRLEQTRALFLRMLED
                     ARVDPPRDARLRAYATQYTIMENANDICRLAIRTCGGQSMLKALPLERLYRDSRCGSL
                     MLPWTAELCLDRIGREALYEPGEED"
     gene            751..2178
                     /locus_tag="VFB53_07075"
     CDS             751..2178
                     /locus_tag="VFB53_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN86532.1"
                     /translation="MVEHWARHAPQKTALRFQQQSWTYAQLWQRIEQATRALGAARGE
                     RIAWLGYNHPDMLALLLAAARLGAILVPLNWRLTAAEHAAILRDCAPRVLYCDAAFAA
                     QARGLGIPLGDWSSKTDARAAGCDEDDLLLVYTSGTTGTPKGAVLQQRALLYNGYNAA
                     HAHDLRQDDHVLSALPMFHVGGLNNQTLPALQAGASVTLHARFEAGAWLHDVAAQRPT
                     LSLLVPATMAAVIAHAEWRTTDLSSLRLLMTGSMVVPLALIDAFHARGVPVGQIYGST
                     ETAPIAIVLQREAARRKAGSTGRPAMHCEVKLVDGEIWVRGPNVMRGYWNDAAASAAV
                     LDDAGWFRTGDLARRDEDGDYWILGRSRELIISGGENVYPAELENVLADCPLIAESAV
                     IGIADAKWGEAACAFVVRKPGAEMDETAVLGLFKDRLARYKHPRRVVFADSLPKNALG
                     KVQKHELKKLLPSPAGLSTIRLGDK"
     gene            2188..2556
                     /locus_tag="VFB53_07080"
     CDS             2188..2556
                     /locus_tag="VFB53_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN86533.1"
                     /translation="MVHHLYGNALVLAPSGRLDHDNCEAFREELAPHLERSAKDGAPI
                     VLDLSGLEYVSSAGLRCFMLAAKQAKAQNSRVVIASMQPVVAEIFQIARFNLVFEAYP
                     RVRDALAALSPGAAAAFDKG"
     gene            2562..3518
                     /locus_tag="VFB53_07085"
     CDS             2562..3518
                     /locus_tag="VFB53_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN86534.1"
                     /translation="MRVRFWGTRGSIPVALTSSDIRDKVALALLRADGRRFASVGEAH
                     QFAEEHLDFALSHTFGGHSACVELETGGDEYFVCDMGSGARPFGAHVLARQSGRPATV
                     NVFMSHVHWDHIMGFPFFGPAYVPGTRIRIHGCHDVMEQAFRLQQAPPCFPVEFAQLS
                     ADIEFVKLAPGRMHEVSGVQVTPAPQMHSGDSYGYRFELQGKSVVYTTDSEHKLENRS
                     QTERFVRFFHGADLVVFDAMYSLADAISVKADWGHSSNIIGVELCQMAQVKHLALFHH
                     EPAYDDAAIEGVLKETRRFEELTRGARTLQVSAAYDGLEIDV"
     gene            3569..5644
                     /locus_tag="VFB53_07090"
     CDS             3569..5644
                     /locus_tag="VFB53_07090"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZN86535.1"
                     /translation="MVLLAAEPPFVFGLRHALFDAYQRLHPRERLSAPAVIVAIDEIA
                     LERHGQWPWPRTRIAQLVAAIAAARPAAIGVDLLFPEPDRFSPGEVAQEVQEMSADVA
                     RRLRSLPSNDRALANAIRERSVVLGIAGLETPDPRFARPPAAPAILIANAPDLALRRF
                     AGHLQSLPAIDQAAAGRGLLSVDPEGGVIRRALVAGRVGEMIVPALSVEMLRVASGAP
                     LALVGRERDEIELRIGDISVPAHADGRFWIRYGPHDPARFVSAAQVLAGEVAAQELES
                     KLVLIGVTGLGLLDFQTTALGERVPGVEIHAQILEQIFDGAYLRRPDVLRTLELGLLA
                     AVAVMLALAVPTLGVGNAIALYAAVIAALAALGISAFAREGLLLDIAWPAIGATVVFG
                     IVLAEALSEADEQRRRLREQAARVSGELESARRIQMGLLPVPRVLFAHEPRFAVDALL
                     EPARTVGGDFYDCFMIDSRRLFFIVADVSGKGLPASLFMALAKTLLKSAARRAADVGG
                     LMVRANAEVSSENPESLFITAFAGILDTRTGTLEFCNAGHEPPIAHRPGGTLERLEHS
                     GGPPLCVMENFAYPVEYRNVAPGEWICVVTDGVTEAMNEAGALYGAARLQKVLAKLPD
                     AVTPTELLAAVRADVGRFVGSAEPSDDLTLLCLRWNGAAAAPQDDELADADLDAAVAG
                     LGDFVASRH"
     gene            complement(5579..6166)
                     /locus_tag="VFB53_07095"
     CDS             complement(5579..6166)
                     /locus_tag="VFB53_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZN86536.1"
                     /translation="MIVALVGALMVLFGAENVGDELVVVLPGPDGKVGTVVVERRGEQ
                     AVLNTAYAASRINEGSTPQALSVGAGEVRKEFRAALGALPGRPKSFLVYFLEGKDEFT
                     DESRVAFDRILAELRERGAPDIVVIGHTDRVGGLQFNDRLSLQRAERVRGELVTLGIP
                     ESRVQVAGRGEREPLVATGDEIPEPRNRRVEISVR"
     gene            complement(6177..6629)
                     /locus_tag="VFB53_07100"
     CDS             complement(6177..6629)
                     /locus_tag="VFB53_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZN86537.1"
                     /translation="MRQTIAVAVTMCALAAPGWALANDVGQVKVAKGTVHVERDGKRV
                     PAKIGMAIRQADTLVTGADGSAGITFSDNSLLSTGPNSVLVVDRYDFNSTTHAGHFDA
                     SLKKGTLAVISGKMVKQSPDAMRVRTPAAIMGVRGTEFVVQVDEPVKK"
     gene            6688..7107
                     /locus_tag="VFB53_07105"
     CDS             6688..7107
                     /locus_tag="VFB53_07105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86538.1"
                     /translation="MNKSRVAQFIARLDQLRAMQAFLEAFCAESGTPRPDCLRLNLVL
                     EELFTNTVKHGHRGDCDAPVWIGLSRTGDAVQVVYEDKAPPFNPYAHLPEDPPDTTLE
                     MRKVGGLGVLLTKQLAASRDYTYLFGRNRIRLTLGQA"
     gene            complement(7111..7197)
                     /locus_tag="VFB53_07110"
     tRNA            complement(7111..7197)
                     /locus_tag="VFB53_07110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7161..7163),aa:Leu,seq:taa)
     gene            complement(7218..7291)
                     /locus_tag="VFB53_07115"
     tRNA            complement(7218..7291)
                     /locus_tag="VFB53_07115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7257..7259),aa:Cys,seq:gca)
     gene            complement(7342..7417)
                     /locus_tag="VFB53_07120"
     tRNA            complement(7342..7417)
                     /locus_tag="VFB53_07120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7382..7384),aa:Gly,seq:gcc)
     gene            complement(7441..8007)
                     /gene="pgsA"
                     /locus_tag="VFB53_07125"
     CDS             complement(7441..8007)
                     /gene="pgsA"
                     /locus_tag="VFB53_07125"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZN86539.1"
                     /translation="MPLNLPNLITWLRIILIPLMIGVFYLPDDWLSYTGRNIAACAIF
                     VVAALSDWLDGYLARALGQTSAFGAFLDPVADKLVVVAALIMLLHLERVEPVVALIII
                     GREIAISALREWMASIGKSKSVAVAFVGKFKTFGQMVAIPLLLYQDDLFGALNAEWLG
                     TILINIAAVLTVISMLVYLRRALPHARG"
     gene            complement(8007..9773)
                     /gene="uvrC"
                     /locus_tag="VFB53_07130"
     CDS             complement(8007..9773)
                     /gene="uvrC"
                     /locus_tag="VFB53_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZN86540.1"
                     /translation="MDDAGFDAKRFVAGLPSLPGVYRMLGASGEALYVGKARDLKKRV
                     SSYFQKSVASPRTRMMLGQVARVEITATRSEAEALLLENNLIKSLAPRYNILFRDDKS
                     YPYLTLSGHRFPRLGFHRGARDARHRYFGPFPHASAVRESIQLLQRVFRLRTCEDSVF
                     ENRSRPCLLHQIRRCTAPCTGLIGAQAYAQDVQHAELFLKGGEDDVMRRLTERMQAAA
                     AARQYEAAAGFRDQVRALSRVQARQYVESDRGVDADVIACVAQGALACVNLAMIRGGR
                     HVGDRSFFPANADAAAPEEVLQAFLEQHYLEQPAPPLVLVSHAVEFDAVVPAQGERRV
                     WLGMAMKNAELALAQRARERGAQEERLAALREALGLPDSVQRIECFDVSHTMGEAAVA
                     ACVVYDRHALQKGEYRRFNLRELAPGDDYAAMRQALARRYARVSAEEGRMPDLILIDG
                     GRGQVSAARLALAETGLHEVAVVGVAKGEARKPGLEELIVAAGERALSLPPTHPGLHL
                     IQQIRDEAHRFAIVGHRARRAKSRTSSALAEIPGIGAQRRQRLLAHFGGLREVQAAAA
                     DEIAQVEGISRPLAERIYKALH"
     gene            9849..10415
                     /gene="efp"
                     /locus_tag="VFB53_07135"
     CDS             9849..10415
                     /gene="efp"
                     /locus_tag="VFB53_07135"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZN86541.1"
                     /translation="MAKVSAVELRVGNLLEWDKRVWRVLKCYHVHVGGRGGAFMQVEM
                     KDIESGTKTNQRFRTEDKIERAFVDARDMQFLYQDGSGFVFMDQENYEQLTLPADFLE
                     GQAGYLLPNAEVQINFYNGRAIGVEIAPSVVLTVTDTEPGIRNATATNTFKPATMETG
                     ITVQVPPFINKGEKIKIDTNQGTYMERG"
     gene            complement(10412..>10926)
                     /gene="nagZ"
                     /locus_tag="VFB53_07140"
     CDS             complement(10412..>10926)
                     /gene="nagZ"
                     /locus_tag="VFB53_07140"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZN86542.1"
                     /translation="GKHFPGHGYAQADSHLAVPTDERSLAEIRRKDLPPYAAAIAAGL
                     AGVMPAHVIYPKVDGEPAGYSRVWLQDVLRGELKFGGLIFSDDLSMAGATTAGDVAAR
                     ARAALAAGCGMVLLCNDPQGQERLLESLAGVPLAQPAQAERMRRRGGARDLRKSVAYR
                     DAQQALEALA"
BASE COUNT         1635 a         3916 c         3717 g         1658 t
ORIGIN      
        1 tggtcagcgg caagaagatc ttcgcgtcgc tctcgggggc tgcggactat tacggcatcc
       61 tctgcaccga gcgcgcggag ggcgagcagg caagccgccg caatacgctc tatctcgcgg
      121 tgcccgccaa tgcgcccggc gtcagcgtgg tcggcgactg ggacccgctc ggcatgcgcg
      181 gcacggtctc gcgcacgctg ctgatgaagg acgtgttcgt tcccgagagc gaactgctga
      241 tgccgcacgg cgtctacttc cgcgccgcga cctcttggcc gcacatgttc ctcacattgt
      301 cgccgaccta tatgggcctc gcgcaggcgg cctacgattt caccgtcgcc tatctgcgcg
      361 gcgaagcccc cgggacgccg ccggtcaagc ggcgcatgtt cccgaccaag cagatcgcgg
      421 tcgccgagat gtgggtgagg ctcgagcaga cgcgcgccct gttcctgcgc atgctggagg
      481 atgcgcgcgt cgatccgccc agggacgcgc gcctgcgcgc ctacgcgacg cagtacacga
      541 tcatggagaa cgcgaacgac atctgccggc tcgccatccg cacctgcggc gggcagtcga
      601 tgctcaaggc gctgccgctg gagcgcctgt accgcgactc gcgctgcggc tcgctgatgc
      661 tgccctggac cgcggagctg tgcctggacc gcatcggccg cgaagccctg tacgaacccg
      721 gcgaggagga ttgagctgga cctttcgcgc atcgtcgagc actgggcgcg gcacgcgccg
      781 cagaagaccg cgctgcggtt ccagcagcag agctggacct acgcgcagct gtggcagcgc
      841 atcgagcagg ccacgcgcgc gctgggcgcg gcgcgcggcg agcgcatcgc ctggctcggc
      901 tacaaccatc cggacatgct ggccctgctc ctcgccgcgg cgcgcctggg cgcgatcctg
      961 gtgccgctca actggcggct gaccgccgcc gagcacgcgg ccatcctgcg cgactgcgcg
     1021 ccgcgcgtcc tgtactgcga tgcggcgttc gccgcccagg cgcgcggcct gggaattccg
     1081 ctcggcgact ggagcagcaa gaccgatgcg cgggccgccg gctgcgacga ggacgatctc
     1141 ctgctggtct acacctccgg caccaccggg acccccaagg gcgccgtgct gcagcagcgc
     1201 gccctgctgt acaacggcta caacgccgcg cacgcgcacg acctgcgcca ggacgaccac
     1261 gtcctctccg cgctgcccat gttccacgtc ggcggcctca acaaccagac gctgccggcg
     1321 ctgcaggcgg gcgccagcgt caccctgcac gcgcgcttcg aggcgggagc gtggctgcac
     1381 gatgtcgccg cgcagcgccc gacgctgagc ctgctggtgc ctgccaccat ggccgccgtg
     1441 atcgcgcatg ccgagtggcg caccacggac ctgtcgtcgc tgcgcctgct gatgaccggg
     1501 tcgatggtgg tgcccctcgc cctgatcgat gcgttccacg cccgtggcgt tccggtcggc
     1561 cagatctacg gctcgacgga aacggcgccg atcgccatcg tgctccagcg cgaggcggcc
     1621 aggcgcaagg ccggcagcac gggcaggccg gcaatgcact gcgaggtcaa gctggtcgac
     1681 ggcgagatct gggtgcgcgg ccccaacgtg atgcgcggct actggaacga cgccgcggcg
     1741 agcgccgccg tgctggatga cgcaggctgg ttcaggaccg gcgaccttgc ccgcagggac
     1801 gaggacggcg actactggat cctgggccgc tcccgggaat tgatcatctc cggcggcgaa
     1861 aacgtgtatc cggcggagct ggaaaacgtg ctggccgact gcccgctgat cgcggaaagc
     1921 gcggtgatcg gcatcgcgga cgccaagtgg ggcgaagccg cctgcgcgtt cgtggtgcgc
     1981 aagcccgggg ccgagatgga cgaaacggcg gtgctcgggc tgttcaagga ccggctggcc
     2041 cgctacaagc acccccggcg cgtggtgttc gcggacagcc tgcccaaaaa cgcactcggc
     2101 aaggtgcaga agcacgagct caagaagctg ttgccgtccc cggcgggttt gtcgacaata
     2161 cgactcggag ataaataaaa aatgcccatg gtccaccacc tgtacggcaa cgcgctggta
     2221 ctcgcgccct cgggccggct cgaccacgac aactgcgagg ccttccgcga ggagctggcg
     2281 ccccacctgg agcgctcggc caaggatgga gcgccgatcg tgctcgacct gtccgggctg
     2341 gagtacgtgt cgagcgccgg cctgcgctgc ttcatgctcg cggcgaaaca ggccaaggcg
     2401 cagaacagcc gcgtcgtcat cgcctcgatg cagccggtgg tggcggaaat cttccagatc
     2461 gcccgcttca acctggtctt cgaggcctat ccccgggtgc gcgacgcgct agccgcgctg
     2521 tcccccggcg ccgccgccgc gttcgacaag ggctagggca agtgcgcgtg cgcttctggg
     2581 gaacgcgcgg ttcgatcccg gtcgccctca cctcctccga catccgcgac aaggtcgcgc
     2641 tcgcgctgct gcgcgccgac ggccggcgct ttgccagcgt cggcgaggcg caccagtttg
     2701 ccgaggagca cctggacttc gcgctgtcgc ataccttcgg cggacattcc gcctgcgtcg
     2761 agctcgagac cggcggcgac gagtactttg tctgcgacat gggcagcggc gcgcggccct
     2821 tcggcgcgca cgtgctcgcg cgccaatccg ggcgcccggc gacggtgaac gtgttcatgt
     2881 cgcacgtgca ctgggaccac atcatggggt tcccgttctt cggccccgcc tacgttcccg
     2941 gcacccgcat ccgcatccat ggctgccacg acgtgatgga gcaggccttc cgcctgcagc
     3001 aggcgccgcc ctgcttcccg gtggaattcg cgcagctctc cgccgacatc gagttcgtca
     3061 agctcgcgcc gggccgcatg cacgaggtct ccggcgtcca ggtgacgccc gcgccgcaaa
     3121 tgcattccgg cgactcctac ggctaccgct tcgagctgca gggcaagtcg gtggtctaca
     3181 ccacggactc ggagcacaag ctggagaacc gctcgcagac cgagcgcttc gtgcgcttct
     3241 tccacggcgc ggacctggtg gtgttcgacg ccatgtactc gctcgccgac gcgatctcgg
     3301 tgaaggcgga ctggggacac tccagcaaca tcatcggcgt cgagctgtgc cagatggcgc
     3361 aggtcaagca cctggcgctg ttccaccacg agcccgccta cgacgatgcg gcgatcgaag
     3421 gggtgctgaa ggagacgcgg cgcttcgagg agctgacgcg cggcgcgcgc acgctgcagg
     3481 tctcggcagc ctacgacggc ctggagatcg acgtctgaag tccgcctggt ccaagcgggc
     3541 cggcccgctg gcgctggtca tcgcactgct ggtcctgctt gccgccgagc cgccgttcgt
     3601 gttcggcctg cgccacgcgc tgttcgacgc ctaccagcgg ctgcatccgc gcgagcgatt
     3661 gagcgccccg gcggtgatcg tcgccatcga cgagatcgcg ctggagcggc atggccaatg
     3721 gccctggccg cgcacgcgca tcgcgcagct ggtcgcggcg atcgccgccg cgcggcctgc
     3781 cgccatcggc gtcgatctgc tgtttcccga gcccgaccgc ttctcgccgg gcgaagtcgc
     3841 ccaggaagtg caggagatgt ccgcggacgt ggcgcggcgg ctgcgctccc tgccttccaa
     3901 cgaccgcgcg ctggccaatg cgatccgcga gcggagcgtg gtactgggca tcgccggcct
     3961 ggaaacgccc gatccgcgct tcgcgcggcc gccggccgcg cccgccatcc tgatcgcgaa
     4021 cgcccccgac ctggcgctgc gccgctttgc cgggcacctg cagagcctgc ccgccatcga
     4081 ccaggccgcc gccgggcgcg ggctgctcag cgtggatccg gagggcggcg tcatccggcg
     4141 cgcgctggtc gccggccgcg tcggcgagat gatcgtgccc gcgctgagcg tggagatgct
     4201 gcgcgtggcg agcggcgcgc cgcttgccct ggtcggccgc gagcgcgatg agatcgaatt
     4261 gcgcatcggc gacatctcgg tgccggcgca tgccgacggc cgcttctgga tccgctacgg
     4321 gccgcacgat ccggcgcgct tcgtctccgc cgcccaggtg ctggccggcg aagtcgccgc
     4381 gcaggagctg gagagcaagc tggtgctgat cggcgtcacc gggctcggcc tgctcgactt
     4441 ccagaccacc gcgctgggcg agcgcgttcc cggcgtcgag atccacgcgc agatcctcga
     4501 gcagatcttc gacggcgcct acctgcggcg gcccgacgtc ctgcgcaccc tggagctcgg
     4561 gctgctcgcc gcggtggcgg tgatgctcgc cctcgccgtg ccgacgctgg gcgtgggcaa
     4621 cgccatcgcc ctgtacgccg ccgtgatcgc cgccctcgcc gcgctcggca tctcggcatt
     4681 cgcgcgcgaa ggactgctgc tcgacattgc ctggcccgcc atcggcgcca ccgtggtgtt
     4741 cggcatcgtg ctggccgagg cgctgagcga ggccgacgag cagcggcggc ggctgcgcga
     4801 acaggcagcg cgcgtcagcg gcgagctgga atccgcgcgc cgcatccaga tgggactgct
     4861 gcccgtcccg cgcgtgctgt tcgcgcacga gccgcgcttc gcggtggatg cgctgctcga
     4921 gccggcgcgc accgtgggcg gcgatttcta cgactgtttc atgatcgatt cgcgccgcct
     4981 gttcttcatc gtcgctgacg tgtccgggaa gggattgccg gcgagcctgt tcatggccct
     5041 ggccaagacc ctgctgaaaa gcgcggcacg ccgcgccgca gacgtcggtg gcctgatggt
     5101 gcgcgccaac gccgaggtgt ccagcgagaa cccggaatcc ctgttcatca ccgccttcgc
     5161 cggcatcctc gacacgcgca ccggcactct ggagttctgc aatgccggcc acgagccgcc
     5221 gatcgcgcat cggccgggcg ggacgctgga gcggctggag cattccggcg gcccgccctt
     5281 gtgcgtgatg gagaatttcg cctaccccgt cgagtaccgc aacgtcgcgc caggcgaatg
     5341 gatctgcgtg gtgaccgacg gcgtcaccga ggccatgaac gaggcgggcg cgctctacgg
     5401 cgccgcgcgg ctgcagaagg tgctggccaa gctgccggat gcggtgaccc cgaccgagct
     5461 gctggcggcg gtgcgcgccg atgtcgggcg cttcgtcggc tcggccgagc cctcggacga
     5521 cctgacgctg ctgtgcctgc gctggaacgg cgccgccgcg gcgccgcagg acgacgagct
     5581 agcggacgct gatctcgacg cggcggttgc ggggctcggg gatttcgtcg ccagtcgcca
     5641 ctagcggctc gcgctcgccg cgccccgcga cctgcacgcg cgactcgggg atgccgagcg
     5701 tcaccagctc gccccgcacc cgctcggcgc gctgcagcga cagccggtcg ttgaactgca
     5761 atccgccgac gcggtcggta tgcccgatca ccacgatgtc cggcgcgccg cgctcgcgca
     5821 gctcggccag gatcctgtcg aaggccacgc gcgactcgtc ggtgaactcg tccttgcctt
     5881 cgagaaagta gaccaggaag gacttcggcc gtcccggcag ggcgccgagc gcggcccgga
     5941 actccttgcg cacctcgccg gcgccgacgc ttagcgcctg cggcgtgctg ccttcgttga
     6001 tgcggctggc cgcgtaggcg gtgttcagca ccgcctgctc gccccgccgc tccaccacca
     6061 ccgtgccgac cttgccgtcc ggccccggca gcacgacgac cagttcgtcg ccgacgtttt
     6121 ccgcgccaaa cagaaccatg agcgccccga cgagcgcgac gatcacggcc cccatttcat
     6181 ttcttgaccg gctcgtccac ctgcaccacg aattcggtgc cccgcacgcc catgatcgcg
     6241 gccggcgtgc gcacgcgcat ggcgtccggc gactgcttga ccatcttgcc ggagatcacc
     6301 gccagcgtgc cctttttcag cgaggcgtcg aagtggcccg cgtgggtggt gctgttgaag
     6361 tcgtaccggt cgacgaccag aacgctgttc ggcccggtgg acagcaggct gttgtcgctg
     6421 aacgtgatgc cggccgagcc gtcggcgccg gtgaccaggg tgtcggcctg gcggatggcc
     6481 atgccgatct tggccggcac gcgcttgccg tcccgctcga cgtgcaccgt tcccttggcg
     6541 accttgacct gcccgacgtc gttggcgagc gcccagcccg gggccgccaa cgcgcacatc
     6601 gtcaccgcta ccgcgatagt ctgacgcata cgccctcccg ctctatgaca gggattctat
     6661 aatgcccggc aggaacccga tagcaccgtg aacaaatccc gagttgcaca gtttattgca
     6721 cgcctggacc agctgcgggc catgcaggcg ttcctggagg cgttttgcgc cgaatccggc
     6781 accccgcgcc cggactgcct gcgcctcaac ctggtgctgg aggagctgtt caccaacacg
     6841 gtgaagcatg gccaccgcgg cgactgcgac gccccggtct ggatcggcct gtcgcgcacc
     6901 ggggacgccg tccaggtggt gtacgaggac aaggcgccgc ctttcaatcc ctacgcccat
     6961 ctgccggagg acccgccgga caccaccctc gaaatgcgca aggttggcgg cctcggcgtg
     7021 ctgctcacca agcagctggc ggcatcgcgc gactacacct acctgttcgg gcgcaaccgc
     7081 atccgcctga ccctcggcca ggcctaggcc tggtgcccgg ggctggattt gaaccagcat
     7141 ggccttgcgg ccgagggatt ttaagtccct tgcgtctacc agtttcgcca cccgggcggc
     7201 gacgcggcgg gaagaattgg aggcgggagt cggaatcgaa ccggcgtcca cggctttgca
     7261 ggccgctgca tgaccactct gccatcccgc ccggggatgc aacgaaaaag ggaaggcctc
     7321 ccagccttcc ctagcctaaa ctggagcggg agacgagtct cgaactcgcg acctcaacct
     7381 tggcaaggtt gcgctctacc aactgagcta ctcccgcgtc atggagcgca aaattatagc
     7441 tcatcccctg gcgtgcggca gggcgcggcg caagtacacc agcatcgaga tcaccgtcag
     7501 caccgccgcg atgttgatca ggatggtgcc gagccactcg gcgttgagcg cgccgaacag
     7561 gtcgtcctgg tacagcagga gcgggatggc gaccatctgg ccgaaagtct tgaacttgcc
     7621 gacgaacgcc accgccacgc tcttcgactt gccgatgctc gccatccatt cgcgcagcgc
     7681 cgagatggcg atctcgcgcc cgatgatgat cagcgccacc accggctcga cgcgctccag
     7741 gtgcagcagc atgatcagcg cggcgaccac caccagcttg tcggcgaccg gatcgaggaa
     7801 cgcgccgaag gcggaggtct ggccgagcgc gcgcgccaga tagccgtcga gccagtcgga
     7861 gagcgccgcc accacgaaaa tcgcgcaggc ggcgatgttc ctgcccgtgt aggacagcca
     7921 gtcgtcgggc aggtagaaca cgccgatcat cagcgggatc aggatgatgc gcagccaggt
     7981 gatcaggttg ggcaggttca gcggcatcag tgcagggcct tgtagatgcg ctcggcgagc
     8041 ggccggctga tgccctcgac ctgcgcgatc tcgtcggcgg ccgcggcctg cacctcgcgc
     8101 aggccgccga aatgcgccag caggcgctgg cgccgctgcg cgccgatgcc cgggatctcg
     8161 gcgagcgccg agctggtgcg cgatttcgcg cgccgcgcgc gatggccgac gatggcgaag
     8221 cggtgcgcct cgtcgcggat ctgctggatc aggtgcagtc cgggatgcgt cggcggcagg
     8281 ctcagcgcgc gctccccggc ggcgacgatc agttcctcca gccccggctt gcgcgcctcg
     8341 cccttggcca cgccgaccac ggcgacttcg tgcaggccgg tctccgccag cgccagccgc
     8401 gccgccgaca cctgtcccct gccgccgtcg atcaggatca ggtccggcat cctgccctcc
     8461 tccgcgctga cgcgcgcata gcgccgcgcc agcgcctgcc gcatggccgc atagtcgtcc
     8521 cccggggcga gctcgcgcag gttgaagcgc cggtactcgc ccttctgcag cgcatgccgg
     8581 tcgtagacca cgcaggcggc gaccgcggcc tcgcccatgg tgtggctgac atcgaagcac
     8641 tcgatgcgct gcaccgagtc cggcaggccg agcgcctcgc gcaacgccgc caggcgctcc
     8701 tcctgcgcgc cgcgctccct ggcgcgctgc gcgagcgcca gctcggcgtt cttcatcgcc
     8761 atgccgagcc atacccgccg ctcgccctgc gccggcacca ccgcgtcgaa ttccactgcg
     8821 tggctcacca gcaccagcgg cggcgcgggc tgctccaggt agtgctgctc gagaaaggcc
     8881 tgcagcacct cctccggcgc cgcggcatcg gcgttcgccg gaaagaagct gcggtcgccg
     8941 acgtgccgcc cgccgcggat catcgccagg ttgacgcagg ccagcgcgcc ctgcgccacg
     9001 caggcgatga cgtcggcgtc cacgccgcgg tccgattcga cgtactgccg cgcctgcacg
     9061 cgcgacaggg cgcgcacctg gtcgcgaaat cccgccgcgg cttcgtactg gcgcgccgcc
     9121 gcagccgcct gcatgcgctc ggtcagtcgc cgcatcacgt cgtcctcgcc ccccttgagg
     9181 aacagctcgg cgtgctgcac gtcctgcgca taggcctgcg cgccgatcag gccggtgcag
     9241 ggcgcggtgc agcggcggat ctggtgcagc aggcaggggc gcgagcgatt ctcgaacacc
     9301 gagtcctcgc aggtgcgcag gcggaacacg cgctgcagca gctggatgct ctcgcgcacc
     9361 gccgaggcgt gcgggaacgg gccgaaatag cggtggcggg cgtcgcgcgc gccgcgatga
     9421 aatcccaggc gcgggaagcg gtgcccgctg agggtgaggt agggatagga cttgtcgtcg
     9481 cggaacagga tgttgtagcg cggcgccagg ctcttgatca ggttgttctc gagcagcagc
     9541 gcctcggctt ccgagcgcgt cgcggtgatc tcgacacgcg ccacctggcc gagcatcatg
     9601 cgggtgcgcg ggctggcgac gctcttctgg aagtaggaac tcacgcgctt cttcaggtcg
     9661 cgcgccttgc cgacgtacag cgcttcgccc gacgcgccga gcatgcggta caccccgggc
     9721 aggctgggca gcccggccac aaagcgcttc gcgtcgaagc cggcatcgtc catggcaagg
     9781 gggattctag cgccccgtgc ccgaatcggt gtagactgcg cgcctttttt caggtcttcc
     9841 ctccgcccat ggccaaggtc tccgcagtcg aacttcgcgt cggcaatctc ctcgagtggg
     9901 acaagcgcgt gtggcgcgtg ctcaagtgct accacgtgca cgtcggcggc cgcggcggcg
     9961 cattcatgca ggtggagatg aaggacatcg agtccggcac caagaccaac cagcgcttcc
    10021 gcaccgagga caagatcgag cgcgcgtttg tcgacgcgcg ggacatgcag ttcctctacc
    10081 aggacggctc gggcttcgtg ttcatggacc aggagaacta cgagcagctg acgctgccgg
    10141 cggatttcct cgaggggcag gccggctacc tgctgcccaa cgccgaggtg cagatcaatt
    10201 tctacaatgg ccgcgccatc ggcgtcgaga tcgcgccctc ggtggtgctc acggtgaccg
    10261 acacggaacc cggcatccgc aacgcgaccg ccaccaacac cttcaagccg gcgaccatgg
    10321 aaaccggcat cacggtgcag gtgccgccct tcatcaacaa gggcgagaag atcaagatcg
    10381 acaccaacca gggcacctac atggagcgcg gctaggccag cgcttcgagc gcctgctgcg
    10441 catcgcggta ggcgacgctc ttgcgcaggt cgcgcgcgcc gccccggcgg cgcatccgct
    10501 cggcctgcgc cggttgggcc agcgggacgc cggcgaggga ttccagcagc cgctcctggc
    10561 cctgcgggtc gttgcacagc agcaccatgc cgcagccggc ggcgagcgcg gcgcgcgccc
    10621 gcgcggcgac gtcgccggcc gtggtggcgc ccgccatcga caggtcgtcg ctgaagatga
    10681 ggcccccgaa cttcagctcg ccgcgcagca cgtcctgcag ccagaccctg gagtagcccg
    10741 cgggttcgcc gtcgaccttc gggtagatga catgcgccgg catcaccccc gccagtccgg
    10801 ccgcgatggc ggcggcataa ggcggcaggt ccttgcgccg gatctcggcg agcgaacgct
    10861 cgtcggtcgg caccgcgagg tgcgagtccg cctgtgcgta gccgtgtccc gggaaatgct
    10921 tgccga
//
LOCUS       DASRIE010000165         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2245629, whole genome shotgun sequence.
ACCESSION   DASRIE010000165 DASRIE010000000
VERSION     DASRIE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245629"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1315)
                     /locus_tag="VFB53_07145"
     CDS             complement(86..1315)
                     /locus_tag="VFB53_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZN86543.1"
                     /translation="MRTAAVVFSACLALSPAAVHGFETADQIPFPSRGAFPAWEGDPL
                     RPWSVFAYGGLMYDSNALRRSTNEQSDIVARYGVGGRTLNRIMGRQRLLLEGFGEYYD
                     FDRFSEIDHFGYGLRGDLLWEIGNDINGAVAYARRRQHADLGEFRSETRVMSTTDRFI
                     VDGGYRFAPDWRVFAGAEHISASRDSAFSPDLEVTTVRSSLTYGTPLGNRLAAEVRGT
                     RGDTRIVDDITLTVLSDKFEEREVAAILVYGLTGQVRVDGRLGHTTREYDTLQAFDFS
                     GGTYRGRVEWLPTQKLIFSVSAYRQLDTAIDIDASHVVRRGAGFGASWAPTFKLVFTA
                     RFVNDRLLYQGDPAVQALGIAQRDETLRVWRFGAGWEPQRRWQVGAGIDVGERTSNRV
                     GRDYDYTQVMLNVRWTY"
     gene            1521..2468
                     /locus_tag="VFB53_07150"
     CDS             1521..2468
                     /locus_tag="VFB53_07150"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZN86544.1"
                     /translation="MVHSGWRRFALVLAAGAALACAAALAGPQRYIARGGVLLPGGML
                     KAEFAAPDPHAAAALVLAFIEKQPSALLVDPPLVVLEGPRLTAPAALGALALLLGGLV
                     LVRRRPAARSEKDLVAALGVPIVAVRPLAAQDLSRQLAAHWFGRGRPVLAVVSAETGE
                     GRTRVAVELARAFSSGGESTLLIDANFRAPGVHRAFGLRNRAGLADFLDGGDTRIAHC
                     AENLSVMVAGRSADDPLELLSRPRMLELLAAAAKRYRVVLVDTPEAALGPDLQLFAAF
                     AGGALVVTQREAGLDGLKTLEGYLRAASARVVGTVLTPA"
     gene            2469..3818
                     /locus_tag="VFB53_07155"
     CDS             2469..3818
                     /locus_tag="VFB53_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HZN86545.1"
                     /translation="MENLSNYRGDDRGYVAQALFERLEGRGSRWRLLGDHGVYPEHAE
                     DLVQLAVSRAELAEMPRAVALFCHDLDLQLVHLAPEDSRAWRFILGWSDEIGRPRFLA
                     VRICSDYCRGLRCYLRAEDLLSGTPETLFGHALVDAVETGAIGDARAAWLCSLWNEDP
                     RGAIERIARFWRSSGHIRLLAQAAKHGEWSAARASLPSLRRALRRGQMPEPGDVLARL
                     GVAARNLVEPARAAIAFMGRETALRSEVMRQVERDLSPLGLTLFEARTHAPRAGLRVV
                     FDGEERDADVVSVRSDRGLPAIVVEVERAILRWLECRVERRYPRALVGENPVAARVLQ
                     FAARHRLPLVQPLMNCHIGCRLGSPVLMPYPFGIVVERGARIGSRVTLMHQVSLLGGP
                     TIEDNVTIGPGAKVIGAVRIGRGATIGANAVVTDDVPSHDTVVVEKRRRERRVVVNV"
     gene            3856..>5225
                     /locus_tag="VFB53_07160"
     CDS             3856..>5225
                     /locus_tag="VFB53_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN86546.1"
                     /translation="MTFYPRSFLALLLAGFTLVILPLVGALAYSAFNTERLAGKSRTA
                     VFNASQAARASRSLVDRIAAIERVAQQMTLFNDPALATDYSRVHRSFKQLAEEIAQLP
                     LDNAQADALNVTVQHEQALFEMITREGGNGQKLDAQAVAGRIDALIDSARDVLAINNR
                     VQDREVERLRVSAEMVQRNIILLLIFSTAVALAIALALTRYIARPIAEIDAAIRQLGG
                     ADFSRAISVRGPEDLRYLGRRLDWLRRRLDEFETQKNRFLRHVSHELKTPLTALREGA
                     ELLNDQVAGPLAPPQRQVVAIMRDNSVKLQRLIEELLDYQRALHAAASLQVQPLQLAE
                     LVRDATAPHELAAQAKGLRLAVEAEPAVVEADAEKLRSIVDNLISNAVKFTPSGGTVT
                     ISAHAVAGEAIIEVLDTGPGVPQEERESIFNLFFRGRTKADGSAVRVKGSGLGLAIAR
                     ELVEAHG"
BASE COUNT          732 a         1795 c         1907 g          791 t
ORIGIN      
        1 caactacggc cgcagcgaaa aaggggggca ggacccttct tccggcgagc aacacgcggc
       61 tccggaaaaa gggtcctgac cccttttagt aggtccagcg cacgttcagc atgacctggg
      121 tgtagtcgta gtcgcggccg acgcggttgg aagtgcgctc gcccacgtcg atgccggcgc
      181 cgacctgcca gcggcgctgc ggctcccagc cggcgccgaa gcgccagacg cgcagcgtct
      241 cgtcgcgctg cgcgatgccc agcgcctgga ctgccggatc gccctggtac aggagccgat
      301 cgttgacgaa gcgcgcggtg aacaccagct tgaaggtcgg cgcccagctc gcgccgaagc
      361 cggcgccccg ccgcaccacg tggctcgcgt cgatgtcgat ggcggtgtcg agctggcggt
      421 aggccgagac gctgaagatc agcttctgcg tcggcagcca ttcgacccgg ccgcggtagg
      481 tcccgccgct gaagtcgaag gcttggagcg tgtcgtattc gcgggtcgta tggccgaggc
      541 ggccgtccac gcgcacctgc ccggtcagtc catagaccag gatcgccgcg acctcgcgct
      601 cttcgaactt gtcgctcagc acggtgaggg tgatgtcgtc gacgatgcgc gtgtcgccgc
      661 gcgtgccgcg cacctccgcc gccaggcggt tgccgagggg cgtaccgtag gtcaggctgc
      721 tgcgcaccgt ggtgacttcg aggtccgggc tgaacgcgct gtcgcggctc gcgctgatat
      781 gctcggcgcc ggcgaacacg cgccagtccg gcgcgaagcg atagccgccg tcgacgatga
      841 agcgatcggt ggtcgacatg acgcgggtct ctgagcggaa ctcgcccagg tcggcgtgct
      901 ggcgccggcg cgcgtacgcg accgcgccgt tgatgtcgtt gccgatctcc cacagcaggt
      961 ctccgcgcaa gccgtagccg aagtggtcga tctcgctgaa gcggtcgaag tcgtagtact
     1021 cgccgaatcc ctcgagcagc agccgctgcc gccccatgat ccggttcagc gtgcgcccgc
     1081 ccacgccgta gcgcgcgacg atgtcggatt gctcgttggt cgagcggcgc agcgcattgc
     1141 tgtcgtacat gaggccgccg taggcgaaca cgctccaggg tcgcagcggg tcgccctccc
     1201 atgccgggaa tgctcccctg gaaggaaaag ggatctggtc ggcggtttca aacccgtgca
     1261 ccgcggccgg cgacagggca aggcaggcgg agaacacgac ggcggcggta cgcatccggc
     1321 ggtgagtcat gcgtcgtttc ttcctcttgt tggttgaaga gggcgcttag tctatgaggg
     1381 tgtagttgca cctgtctgcc gtcagggaac tacaaacgga ctgtcgtcgt ccggcgacga
     1441 tcccccggga cgggggtagg caaagtcgtt gatttgcaac gcaccgccca ttggcatgca
     1501 cgttgcaggt ggcggtaccc atggtccatt ctgggtggcg tcggttcgcg ctggtgcttg
     1561 cggcgggcgc ggcgctcgcc tgcgcggccg cgctcgccgg gccgcagcgc tacatcgcgc
     1621 gcggcggcgt gctcctgccc ggcggcatgc tgaaggcaga gttcgccgct ccggatcccc
     1681 atgccgccgc cgccctcgtc ctcgccttca tagagaaaca gccatccgcg ctgctggtgg
     1741 atccgccgct cgtggtgctc gaagggccgc ggctcactgc gcccgcggcg ctcggcgccc
     1801 tggcgctgct gctgggcggg ctggtgctgg tgcgccgccg gccggcggcg cgctcggaga
     1861 aggacttggt cgccgccctc ggcgtgccga tcgtggcggt gcggccgctg gccgcgcagg
     1921 atctctcgcg ccagctcgcc gcgcactggt tcgggcgcgg ccggccggtg ctcgcggtgg
     1981 tgagcgccga gaccggcgag ggccgcacgc gggtggcggt cgagctcgcg cgcgcattct
     2041 cctcgggcgg cgaatccacg ctgctcatcg acgcgaactt ccgcgcgccg ggcgtgcacc
     2101 gcgcgttcgg gctgcgcaac cgcgcggggc tggccgactt cctggacggc ggcgacacgc
     2161 gcatcgcgca ttgcgccgag aacctttcgg tgatggtggc ggggcgttcg gccgacgatc
     2221 cgctcgagct cctgagccga ccgcgcatgc tggagctgct cgccgcggcg gcgaagcgct
     2281 accgcgtggt gctggtcgac actcccgagg cggcgctcgg cccggacctg cagctcttcg
     2341 cggctttcgc cggcggcgcg ctggtggtga cgcagcgcga ggccgggctg gacgggctga
     2401 agacgctcga ggggtacctg cgcgccgcat ccgcgcgagt ggtcggcacg gtgctcaccc
     2461 cggcctagat ggaaaacctt tcaaactacc gcggggatga ccgcggctac gtcgcccagg
     2521 ccttgttcga gcgcctggag ggacggggca gccgctggcg cctgctcggc gatcacgggg
     2581 tctatcccga gcatgcggag gatctggtcc agctcgcggt atccagggcc gagcttgccg
     2641 agatgccgcg cgcggtggcg ctcttctgcc acgacctcga cctgcagctc gtgcacctcg
     2701 cgccggaaga cagccgcgcg tggcgcttca tcctcggctg gagcgacgag atcgggcgcc
     2761 cccgcttcct cgcggtacgg atttgcagcg actactgccg cgggctgcgc tgctacctgc
     2821 gcgccgagga cctgctctcc ggcacgccgg agaccctctt cggccacgcc ctggtcgacg
     2881 ccgtggaaac cggcgcgatc ggcgacgcgc gcgcggcctg gctgtgctcg ctctggaacg
     2941 aggacccgcg cggtgcgatc gagcgcatcg cgcgcttctg gcgctcgagc ggccacatcc
     3001 ggcttctggc gcaggcagcg aagcacggcg aatggagcgc ggcgcgcgcg agcctgccgt
     3061 cgctgcgccg cgcgctgcgg cgcggccaga tgccggagcc gggcgacgtg ctcgcgcgcc
     3121 tgggggtggc ggcgcgcaac ctggtggagc cggcgcgcgc cgcgatcgcg ttcatgggac
     3181 gcgagaccgc gctgcgctcg gaggtcatgc gccaggtcga gcgcgacctg tcgccgctcg
     3241 gcctgacgct gttcgaggcc cgcacccacg cgccgcgcgc cgggctgcgt gtggtgttcg
     3301 acggagagga aagggacgcc gacgtggtct cggtgcgcag cgaccgcggg ctcccggcga
     3361 tcgtggtcga ggtcgagcgc gcgatcctgc gctggctcga gtgccgggtc gagcgccgct
     3421 acccgcgcgc gctggtggga gaaaacccgg tcgcggcccg ggtcttgcag ttcgccgcgc
     3481 ggcatcgcct gcccctggtc cagcccctga tgaactgcca catcggctgc cggctgggct
     3541 cgccggtgct gatgccctat ccgttcggca tagtggtgga acgcggcgcg cgcataggca
     3601 gccgggttac gctcatgcac caggtcagcc tgctgggcgg gccgaccatc gaggacaacg
     3661 tgaccatcgg cccgggggcg aaggtgatcg gcgcggtgcg catcgggcgc ggcgccacca
     3721 tcggcgccaa cgcggtggtc acggacgacg tgccgtcgca cgatacggtc gtcgtggaaa
     3781 agcgacgtcg ggagagaaga gtcgtcgtga acgtgtgaca ttggccccgg tccaatgtcg
     3841 tggaaaactc acagcatgac tttttacccg cgctcgttcc tggcgctgtt gctcgccggc
     3901 ttcaccctcg tgatcctgcc gctggtcggc gcgctcgcgt attcggcgtt caacaccgag
     3961 cgcctcgccg gcaagagccg caccgcggtg ttcaacgcct cgcaggccgc gcgcgccagc
     4021 cgctcgctgg tcgaccgcat cgcggcgatc gagcgcgtcg cgcagcagat gacgctgttc
     4081 aatgatccgg cgctcgccac cgactattcg cgcgtgcacc gcagcttcaa gcagctcgcc
     4141 gaggagatcg cgcagctgcc gctcgacaac gcgcaggccg acgcgctgaa cgtcacggtg
     4201 cagcacgagc aggcgctctt cgagatgatc acgcgcgagg gcggcaacgg gcagaagctc
     4261 gacgcgcagg cggtggcggg gcgcatcgac gcgctgatcg acagcgcgcg cgacgtgctc
     4321 gcgatcaaca accgcgtgca ggaccgcgag gtcgagcgcc tgcgggtgag cgcggagatg
     4381 gtccagcgca acatcatcct gctgctcatc ttcagcaccg cggtggcgct cgccatcgcg
     4441 ctcgccctca cgcgctacat cgcgcggccg atcgcggaga tcgacgcggc gatccgccag
     4501 ctcggcggcg cggacttctc gcgcgcgatc tcggtgcgcg ggccggagga cctgcgctac
     4561 ctgggccgca ggctcgattg gctgcgccgg cggctggacg agttcgagac gcagaagaac
     4621 cgcttcctgc gccacgtctc gcacgagctg aagacgccgc tcaccgcgct gcgcgagggc
     4681 gcggagctgc tgaacgacca ggtcgccggg ccgctcgcgc cgccgcagcg ccaggtcgtc
     4741 gccatcatgc gcgacaacag cgtgaagctg cagcgcctga tcgaggagct gctcgactac
     4801 cagcgtgcgc tgcacgcggc ggcgtcgctg caggtgcagc cgttgcagct cgccgagctg
     4861 gtgagggacg ccacggcgcc gcacgagctc gcggcgcaag cgaagggcct gcgcctagcg
     4921 gtcgaggccg agccggcggt cgtggaggcc gatgcggaaa agctgcgctc gatcgtcgac
     4981 aacctgatct cgaacgcggt gaagttcacg ccctcgggcg gcacggtgac gatctcggcg
     5041 cacgccgtcg ccggcgaggc gatcatcgaa gtgctcgata ccggccccgg ggtgccccag
     5101 gaggagcgcg agtcgatctt caacctgttt ttccggggga gaaccaaggc ggacggcagc
     5161 gcggttcgcg tcaagggcag cggcctgggc ctcgccatcg cgcgcgagct ggtcgaggcg
     5221 cacgg
//
LOCUS       DASRIE010000166         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1989135, whole genome shotgun sequence.
ACCESSION   DASRIE010000166 DASRIE010000000
VERSION     DASRIE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989135"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2978
                     /locus_tag="VFB53_07165"
     CDS             21..2978
                     /locus_tag="VFB53_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN86547.1"
                     /translation="MDWTQLIEALLLVAAAGVVLASWMQARLRDRRATLALRISEERF
                     RNLTALSADWFWECDTEYRITWLTGGPAVLGFFGAGLAFGKRLWELPGVELDGRAFTA
                     HLEQLRRRLPFFELELARTDAAGARHTHLVSGQPRHDAAGNFLGYRGVGRDVTERRRG
                     ERELAEAKERLELALEGGSLAVWDCEIGSDRIYLSEGWARLLGDAPKARNVRSADLLQ
                     HVHPQDAVAARAAFVGAIKGTTPAYLAEYRLRMRDGDWKWVLSTGRVTERDAGGRALR
                     ISGVTADIDARKHAEGELAQAKERLQLALEGSGASLWDTDLQSGKVYLSEGWAEFLGL
                     PRAPTATTVEALIQGVHEEDLPRALEQQRQAVTGASADYAEEHRFRAADGAWKWLLSR
                     GKVTERDAAGRALRMTGINLDITARKLTEQALQEVEKRYRDVVDASGEYVWETDAQWR
                     YTYLSERVESVLGYPRAALLGRTPREFMPLGEARALDAWFERNAQEGQPFRGLQHRSI
                     ARSGQVIWQQVSGMPVRDAQGLLAGYRGTGADITASKLAEDRIQYLATRDALTGLPNR
                     ALLADRARQAILAAARGRGMLAVLLVDLDRFKLVNDSLGHQAGDALLRAVGERLQGTL
                     RREDTLARLGGDEFVLLWDRLKGAPEAALVAQRIQGILQRPFEIDARALNVTASIGIS
                     IYPEDGRDFSELLKNADTALYDAKDAGRNSMRFFSPQLHARAAARLTLESDLRGALAR
                     GELVLHWQPVLRQRRIVGAEALVRWNHPVRGLLLPDTFIPAAEESGLIRAVGEWTLAR
                     ALAQAGAWRRSLPGEPWVSMNVSALELAQGAAYVERLREALATHGVAAGRVELEVTER
                     VLMAHLPENIETLRQIGALGVRLAIDDFGTGYSSLAYLRRLPIDKLKIDRGFLRELDS
                     SSGDAMIVRTIAAMARGLGLQVAAEGVETESQLRELLAVGCDEWQGHLYSAPLEAAAF
                     ERLALAGRLAAAG"
     gene            complement(2994..3803)
                     /locus_tag="VFB53_07170"
     CDS             complement(2994..3803)
                     /locus_tag="VFB53_07170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86548.1"
                     /translation="MRLSQLVLNLRRNLPPDPTRSSSLERVLRRAGRLAGWLRADLNL
                     LAALYGTDKYGEHDYTPVYEELMRTARGKPVRLLEIGVGGYAGALGGESLRMWESYFR
                     RGRITGIDLYDKTALSGGRVTVRQCSQVDAPQLGALAQELGPFDFIIDDGSHLNTHQI
                     ETFRILWPHLSDGGAYIVEDVQTSYWPAYGGGPLGTEGYRKSCMQWFKDYADSVNLPE
                     FLAPAPGALDPGLLSIAFHHNLIVLKKDLAARYSNANLDLPEARRALLQPG"
     gene            complement(3834..>4061)
                     /locus_tag="VFB53_07175"
     CDS             complement(3834..>4061)
                     /locus_tag="VFB53_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86549.1"
                     /translation="TMTNGRIRGPVAITLRASRLDLAGVRVEGRDAAVLGQDVGPAPA
                     SSVVFSVSRVRSPHTDGAMHGYMVVVSGKPL"
BASE COUNT          557 a         1423 c         1542 g          539 t
ORIGIN      
        1 cggctaggga cgcgggcgcg atggactgga cgcagctgat cgaggcgctc ctgctggtcg
       61 cggcagccgg ggtggtgctc gcatcctgga tgcaggcgcg gctgcgcgac cggcgcgcca
      121 ccctcgccct gcgcatcagc gaagagcgct tccgcaatct caccgcgctg tcggcagact
      181 ggttctggga gtgcgacacc gagtaccgca tcacctggct caccggcggc ccggcggtgc
      241 tcggcttctt cggtgcgggc ctcgccttcg gcaagcggct gtgggagctg cccggggtgg
      301 agctcgacgg gcgcgcgttc accgcgcacc tggagcagct gcgccggcgc ctgccgtttt
      361 tcgagctgga actggcgcgc accgacgccg cgggcgcgcg ccacacgcac ctggtgtcgg
      421 ggcagccgcg gcacgacgcc gcgggcaatt tcctcggcta ccgcggcgtc ggccgcgacg
      481 tcaccgagcg gcgccgcggc gagcgcgagc tggccgaggc gaaggaacgc ctcgaactcg
      541 ccctggaagg cggcagcctg gcggtgtggg attgcgagat cggcagcgac cgcatctacc
      601 tgagcgaggg ctgggcgcgg ctgctgggcg acgcgcccaa ggcgcgcaat gtgcgctcgg
      661 cggacctgct gcagcacgtg catccgcagg acgccgtcgc ggcgcgcgcc gcgttcgtgg
      721 gcgcgatcaa gggcacgacc cccgcgtacc tcgccgagta ccggctgcgc atgcgcgacg
      781 gcgactggaa atgggtgctc tcgaccgggc gcgtcaccga gcgcgacgcc ggcgggcgtg
      841 cgctgcgcat ttcgggcgtc acggcggaca tcgacgctcg caagcacgcc gagggcgagc
      901 tggcgcaagc caaggagcgc ttgcagctgg cgctggaggg ctcgggcgcc agcctgtggg
      961 acaccgacct gcagagcggc aaggtgtacc tgagcgaggg ctgggccgaa ttcctcggct
     1021 tgccgcgcgc gcccaccgcg accacggtcg aggcgctgat ccagggcgtg cacgaggaag
     1081 acctgccgcg cgcgcttgag cagcagcgcc aggcggtgac gggcgcaagc gccgactatg
     1141 cggaggagca ccgcttccgc gccgcggacg gcgcctggaa gtggctactg tcgcgcggca
     1201 aggtcaccga gcgcgacgcg gcggggcgcg cgctgcgcat gaccggcatc aacctggaca
     1261 tcaccgcgcg caagctcacc gagcaggcgt tgcaggaagt cgagaagcgc taccgcgacg
     1321 tggtggacgc ctcgggcgag tacgtctggg aaaccgacgc gcagtggcgc tacacctacc
     1381 tgtcggagcg cgtcgagtcg gtgctcggct acccgcgcgc cgcgctgctg gggcgcacgc
     1441 cgcgcgagtt catgccgctg ggcgaggcgc gcgcgctcga tgcctggttc gaacgcaacg
     1501 cgcaggaagg ccagccgttc cgcggcctgc agcaccgctc catcgcgcgc tcgggccagg
     1561 tgatctggca gcaggtgagc ggcatgccgg tgcgcgacgc gcaggggctg ctggccggct
     1621 accgcggcac cggcgccgac atcaccgcga gcaagctggc cgaggaccgc atccagtacc
     1681 tcgccacgcg cgacgcgctc accggcctgc ccaaccgcgc gctgctcgcc gaccgggcgc
     1741 ggcaggcgat cctcgccgcg gcgcgcggcc gcggcatgct ggcggtgctg ctggtcgacc
     1801 tggaccgctt caagctggtc aacgactcgc tcggccacca ggccggcgac gcgctgctgc
     1861 gcgccgtggg cgagcgcctg cagggcaccc tgcgccgcga ggacacgctg gcgcggctgg
     1921 gcggcgacga gttcgtgctg ctgtgggacc ggctgaaggg cgcgcccgag gcggcgctgg
     1981 tggcgcagcg catccagggc atcctgcagc ggccgttcga gatcgacgct cgcgcgctca
     2041 acgtcaccgc ctcgatcggc atcagcatct atccggagga cgggcgcgat ttcagcgagc
     2101 tgctgaagaa cgccgacacg gcgctgtacg acgccaagga cgccggccgc aacagcatgc
     2161 gcttcttctc gccgcagctg catgcgcgcg ccgccgcgcg cctcacgctg gagagcgacc
     2221 tgcgcggggc tctggcgcgc ggcgagctgg tgctgcactg gcagccggtg ctgcgccagc
     2281 gccggatcgt gggcgcagag gcgctggtgc gctggaacca cccggtgcgc gggctgctgc
     2341 tgcccgacac cttcatcccg gcggcggagg agagcgggct gatccgcgcc gtcggcgagt
     2401 ggacgctggc gcgcgccctg gcgcaggcgg gcgcctggcg gcgcagcctg ccgggcgagc
     2461 cctgggtgtc catgaacgtg tcggcgctcg aactggcgca gggcgcggcc tacgtcgagc
     2521 gcctgcgcga ggccctggcg acgcacggcg tggccgccgg ccgcgtcgag ctggaagtga
     2581 ccgagcgggt gctgatggcg cacctgccgg agaacatcga gacgctgcgc cagatcggcg
     2641 cgctgggcgt gcgcctggcg atcgacgatt tcggcaccgg ctactcgagc ctcgcctacc
     2701 tgcggcgcct gccgatcgac aagctgaaga tcgaccgcgg cttcctgcgc gagctcgact
     2761 cgagcagcgg cgacgccatg atcgtgcgca ccatcgcggc gatggcgcgc ggcctggggc
     2821 tgcaggtggc ggccgagggc gtggagaccg agtcgcagct gcgcgagctg ctggcggtgg
     2881 gctgcgacga atggcagggc cacctgtaca gcgcgccgct cgaggcggcg gccttcgagc
     2941 gcctggcgct ggcgggccgc ctggctgcgg cgggctagcc cggctgcggc gggctagccc
     3001 ggctgcagca gcgcgcgccg cgcctcgggc aggtcgaggt tggcgttgga atagcgcgcc
     3061 gcgaggtctt tcttcagcac gatcaggttg tggtggaagg cgatgctcag caatccgggg
     3121 tcgagcgcgc ccggcgccgg cgcgaggaat tcgggcaggt tgacgctgtc ggcgtagtcc
     3181 ttgaaccact gcatgcacga cttgcggtag ccctcggtcc ccagcggccc gccgccgtag
     3241 gcgggccaat aggacgtctg cacgtcctcc acgatgtagg cgccgccgtc gctgaggtgc
     3301 ggccagagga tccggaaagt ctcgatctgg tgggtgttca ggtggctccc gtcgtcgatg
     3361 atgaagtcga acggccccag ctcctgcgcc agggcgccga gctgcggggc gtccacctgc
     3421 gagcactggc gcaccgtcac gcgccccccc gagagcgcgg tcttgtcgta caggtcgatg
     3481 ccggtgatgc gcccgcggcg gaagtagctc tcccacatgc gcagcgactc gccgcccagg
     3541 gcgccggcgt agccaccgac gccgatttcc agcaggcgca ccggcttgcc gcgcgcagtg
     3601 cgcatcagtt cctcgtacac cggcgtgtag tcgtgctcgc cgtatttgtc ggtgccatag
     3661 agcgcggcca gcaagttgag gtcggcgcgt agccacccgg cgaggcgccc cgcgcggcgc
     3721 agcacgcgct ccaggctgga ggagcgcgtc gggtccggcg gcaggttgcg ccgcaggttg
     3781 agcaccagct ggctgaggcg catgcccact gcgctatctt atggcagcgc cggctacagc
     3841 ggcttgccgc tcaccacgac catgtagccg tgcatcgcgc cgtcggtatg ggggctgcgc
     3901 acccggctga cggaaaacac caccgacgag gcgggcgcag gcccgacgtc ctgccccagc
     3961 accgccgcgt cccggccctc gacgcgcacg ccggccaggt cgagccggct ggcgcgcagc
     4021 gtgatcgcca ccggcccgcg gatgcgcccg ttggtcatgg t
//
LOCUS       DASRIE010000167         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_945179, whole genome shotgun sequence.
ACCESSION   DASRIE010000167 DASRIE010000000
VERSION     DASRIE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945179"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /gene="sctR"
                     /locus_tag="VFB53_07180"
     CDS             <1..528
                     /gene="sctR"
                     /locus_tag="VFB53_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HZN86550.1"
                     /translation="NALGLQQVPPNVVLNGLALVLSVYVMYPVGTEMAAKLQGVKDIG
                     NSTQGMMKAADLAKEPLRDFLVKHSRPIERAFFFRTAQKALKPEQAATLTDRDFLVIV
                     PAFTVSELSVAFEIGFLIFLPFLVIDLVISNILMAMGMMMLSPTTVSMPFKLLLFVLI
                     DGWVKLTHGLILTYV"
     gene            542..814
                     /gene="sctS"
                     /locus_tag="VFB53_07185"
     CDS             542..814
                     /gene="sctS"
                     /locus_tag="VFB53_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HZN86551.1"
                     /translation="MIHGDILQLTQQALWFVLLLSAPPIIVAAVVGLVIAFVQAATQL
                     QEQTLQYVAKFFAIVITLFITASMLGGGLYQFGDRVFTEFAGLVRR"
     gene            814..1617
                     /gene="sctT"
                     /locus_tag="VFB53_07190"
     CDS             814..1617
                     /gene="sctT"
                     /locus_tag="VFB53_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HZN86552.1"
                     /translation="MDFGAFGPLSDAVLLLGLAATRVAVAFLLVPLFTADLIPALVRN
                     AMFIAIATLSIAVQPSSPAPLALDSTLQWVSLFAKEAFIGGAIGLFFAGTMWAFEAAG
                     QLIDNKVGTTQAQIVDPLSGHQTSLNGALFARLASWVFMAGGGFMLMIGAVLESYALW
                     PVRQPLPALAESGVRLFESAFGRIMLMTVLIAGPAIVLLYLVEGILGLVNRFAQQLNV
                     TFLAMSLKAMAATWIIWVQLASLVQLLQDDLLAQGGAVIGMLRRLFAGS"
     gene            complement(1719..2054)
                     /locus_tag="VFB53_07195"
     CDS             complement(1719..2054)
                     /locus_tag="VFB53_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86553.1"
                     /translation="MHFVEHHEGDLRIYAGAIEAAPEGYHAAVVVHRVRPGDGAAPQE
                     VFREDRVCGGYAWPSPEAAMAFAVQRARSVMRREAHPHTHGLSDAAKAAAADGRSVGR
                     RQRPARLAA"
     gene            complement(2178..2948)
                     /gene="xth"
                     /locus_tag="VFB53_07200"
     CDS             complement(2178..2948)
                     /gene="xth"
                     /locus_tag="VFB53_07200"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN86554.1"
                     /translation="MKIATYNVNGIGSRLPRLLEWLRETAPDVACLQELKTPDATFPI
                     AAIEEAGYGAIWTGQKSFNGVAILAKGQSPQLLRRGLPGDPDDLHSRYIEGEVEGVTI
                     ASIYLPNGNPQPGPKFDYKLAWFERLVRHAAELLATGRPTVLAGDYNVVPTDEDIYNP
                     ASWRNDALLQPESREAYRRLLAQGWTDAVRAAHPGERIYTFWDYFRNRWQRNAGLRID
                     HLLLSPDVAPRLADANVDREQRGKEKASDHAPTWVVLR"
     gene            complement(3031..3249)
                     /locus_tag="VFB53_07205"
     CDS             complement(3031..3249)
                     /locus_tag="VFB53_07205"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HZN86555.1"
                     /translation="MANEQEPTSGRIESTARIDMLSDTDVAYWCRVLDVDHSELRRAV
                     QKVGPRADAVVRCLGAKRTPRPEFDTGF"
     gene            complement(3344..3922)
                     /locus_tag="VFB53_07210"
     CDS             complement(3344..3922)
                     /locus_tag="VFB53_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HZN86556.1"
                     /translation="MKLKMAENSLFAILLRSRWWVSFLLALATALVARLVLPKDYEPL
                     AVFSALPFLVVGAVAAWKQFQQPSPARVQDTLRAVGDMPWAQFADALEEAFRADGHAV
                     ARLPGPAADFEVTRAGRVTLVSGRRWKAARTGVEPLRELHAAMTARDAASGIHVCLGE
                     PSDAAVAFARANGIEILRGPALAVLLRRMAPR"
     gene            complement(3960..>4791)
                     /locus_tag="VFB53_07215"
     CDS             complement(3960..>4791)
                     /locus_tag="VFB53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN86557.1"
                     /translation="PAPGAAPMLRRRAGPLGAIPVVGMGTFDVGDDAGELARRREVLR
                     RFFAAGGGMIDSSPMYGRAEAVLGELLPGLPHAGRLVAATKVWTPFERLGPRQLEHSL
                     ALWRVPRFDVVLVHNLLEWRAHLRLLRRWKEGGRARAIGVSTSHGSRHDEAARIVRSE
                     PLDVLQITYNLADASAEPLMQLAAERGMAVVVNRPFDGGALFDRIGRTPLPAWAPELG
                     CDTWARFLLAWVVSHPVVACAIPATRQPAHMDDNMGTARTPLPDAAARRRMQQALASL
                     "
BASE COUNT          607 a         1704 c         1756 g          724 t
ORIGIN      
        1 aacgcgctcg gcctgcaaca ggtgccgccc aacgtggtac tcaacggcct ggccctggtg
       61 ctcagcgtct atgtgatgta cccggtgggc accgagatgg cggccaagct gcaaggcgtg
      121 aaggacatcg gcaacagcac gcagggcatg atgaaggcgg ccgacctggc caaggagccg
      181 ctgcgcgatt tcctcgtcaa gcacagccgc ccgatcgagc gtgccttctt cttccgcacc
      241 gctcagaagg ccctcaagcc cgaacaggcg gccaccctga ccgaccgcga cttcctggtc
      301 atcgtgccgg cgttcaccgt gagcgagctg tcggtggcgt tcgagatcgg cttcctgatc
      361 ttcctgccgt tcctggtgat cgacctcgtc atctccaaca tcctcatggc gatgggcatg
      421 atgatgctgt cgcccaccac cgtgtcgatg ccgttcaagc tgctgctgtt cgtgctgatc
      481 gacggctggg tgaagctcac ccacggcctc atcctcacct acgtctgacg gggcgcgcct
      541 catgatccac ggcgacatcc tccagctcac ccagcaggcg ctctggttcg tgctgctgct
      601 gtcggccccg cccatcatcg tggcggcggt ggtcggcctg gtgatcgcgt tcgtgcaggc
      661 ggccacgcag ctgcaggagc agacgctgca gtacgtggcg aagttcttcg cgatcgtcat
      721 cacgctgttc atcaccgcgt cgatgctggg cggcgggctg taccagttcg gcgaccgcgt
      781 gttcacggag ttcgcggggc tggtgaggcg ctgatggact tcggcgcgtt cgggccgctg
      841 tcggacgcgg tgctcctgct cggcctggcc gcgacgcgcg tcgccgtggc cttcctgctc
      901 gtgccgctgt tcactgccga cctcattccg gcgctggtgc gcaacgcgat gttcatcgcc
      961 atcgcgacgc tcagcatcgc ggtgcagccg tcgtcgccgg cgccgctcgc gctcgacagc
     1021 acgctgcagt gggtgagcct gttcgccaag gaagccttca tcggcggcgc gatcgggctg
     1081 ttcttcgcgg gcacgatgtg ggccttcgag gccgccgggc agctgatcga caacaaggtc
     1141 ggcaccacgc aggcgcagat cgtcgatccg ctgtcgggcc accagacctc gctcaacggc
     1201 gccctgttcg cgcggctcgc gagctgggtg ttcatggccg gcggcggctt catgctgatg
     1261 atcggcgcgg tgctcgagag ctacgcgctg tggccggtgc gccagccgct gcccgcgctc
     1321 gcggagtccg gcgtgcgcct gttcgagagc gcgttcggcc gcatcatgct gatgaccgtg
     1381 ctgatcgcgg gccccgcgat cgtgctgctc tacctcgtcg agggcatcct cgggctggtc
     1441 aaccggttcg cgcagcagct caacgtcacc ttcctcgcga tgtcgctgaa ggcgatggcc
     1501 gccacctgga tcatctgggt ccagctcgcg agcctggtgc agctgctgca ggacgacctg
     1561 ctggcccagg ggggcgccgt gatcgggatg ctgcggcggt tgttcgcggg gtcgtagctc
     1621 ggccggcgcc gcgcgcgggc gggcgtcgat gccggcgggc gtcgatgcgg gcgccccgcc
     1681 ggatgccgcc ggcggcgggt gacggaacgc cgcttcgctc aggcggccag gcgcgccggg
     1741 cgctggcgcc gcccgacgct gcggccgtcg gcggccgccg ccttggcggc gtcggagagg
     1801 ccgtgcgtgt gcggatgggc ctcgcggcgc atcacgctgc gcgcacgctg caccgcgaac
     1861 gccatcgccg cttccgggct cggccaggcg tagccgccgc acacgcggtc ctcgcggaag
     1921 acctcctgcg gcgcggcgcc gtcgccgggc cgcacccggt gcacgacgac cgccgcgtgg
     1981 tagccctcgg gggccgcttc gatcgcgccg gcgtagatgc gcaggtcgcc ttcgtggtgc
     2041 tcgacgaaat gcatggggcc tcctggcttc ggatgccctg ggtatgcatg cggcgccgca
     2101 ggcgttcagt gaagtcggcg cgcgagcgct cgacagcgcg cccttgcgtg acgcggttct
     2161 cgcttcggcg cgccccgtca gcgcagcacg acccacgtgg gggcgtggtc gctcgccttc
     2221 tccttgccgc gctgctcgcg gtcgacgttg gcgtcggcca gccgcggcgc gacgtcgggg
     2281 ctgagcagca ggtggtcgat gcgcagcccc gcgttgcgct gccagcggtt gcggaaatag
     2341 tcccagaagg tgtagatgcg ctcgccggga tgggcggcgc gcaccgcgtc ggtccagccc
     2401 tgcgccagca ggcgccggta cgcctcgcgg ctttcgggct gcagcagcgc gtcgttgcgc
     2461 cacgaggccg ggttgtagat gtcctcgtcg gtgggcacga cgttgtagtc gccggccagc
     2521 acggtgggcc ggccggtcgc cagcagttcg gccgcatggc ggaccaggcg ctcgaaccag
     2581 gccagcttgt agtcgaactt ggggccgggc tgcgggttgc cgttgggcag gtagatcgag
     2641 gcgatggtca cgccctccac ctcgccttcg atgtagcggc tgtgcaggtc gtcggggtcg
     2701 ccgggcagtc cgcggcgcag cagctgcggc gactgaccct tggcgaggat ggccacgccg
     2761 ttgaaggact tctgcccggt ccagatggcg ccgtagccgg cttcctcgat cgccgcgatg
     2821 gggaacgtgg cgtcgggcgt cttcagctcc tgcaggcagg cgacatcggg cgcggtctcg
     2881 cgcagccatt cgagcaggcg cggcaggcgg ctgccgatgc cgttcacgtt gtaggtggcg
     2941 atcttcatgg gcgcggatga tggcatgcca ccgcgccccg gaccgtggcc gccccggggg
     3001 acctgcccgc gccgcgcggg cgcgcgagcg tcagaagccg gtgtcgaact ccggccgcgg
     3061 cgtgcgcttc gcgccgaggc agcgcacgac ggcgtccgcg cgcgggccca ccttctggac
     3121 cgcgcggcgc agctcgctgt ggtccacgtc gagcacgcgg caccagtacg cgacgtcggt
     3181 gtcgctcagc atgtcgatgc gcgcggtgga ctcgatgcgc ccgctggtcg gctcttgctc
     3241 gttggccatg tcgtctcctg gaggggtctc cacgggcctg acgctagcac cacgccgggc
     3301 gggggcgcaa tgcccctgat gcttggaaac attcgcatcc gggctaacgt ggagccatgc
     3361 ggcgcagcag caccgccagc gccggcccgc gcaggatctc gatgccgttc gcccgcgcga
     3421 aggccaccgc cgcgtcgctg ggctcgccga ggcagacgtg gatcccggaa gcggcatcgc
     3481 gcgccgtcat cgccgcgtgc agttcgcgca ggggctcgac gccggtgcgc gccgccttcc
     3541 agcgccgccc gctcaccagc gtgacgcggc cggcgcgcgt cacctcgaag tcggcggcgg
     3601 gtccgggcag ccgcgcgacc gcgtggccgt cggcgcggaa cgcctcctcg agcgcgtcgg
     3661 cgaactgcgc ccacggcatg tcgcccaccg cgcgcagcgt gtcctgcacg cgcgccgggc
     3721 tgggctgctg gaactgcttc cacgcggcga ccgcgccgac caccaggaac ggaagcgcgg
     3781 agaacaccgc gagcggctcg tagtccttcg gcagcacgag ccgcgcgacc agcgccgtcg
     3841 cgagcgcgag caggaagctc acccaccagc gcgagcgcag caggatcgcg aacagcgagt
     3901 tctcggccat cttcagcttc acggtcgccc cccttgcgca gccggagtat cggcgctcgt
     3961 cacagcgacg ccagcgcctg ctgcatgcgc cgccgcgccg ccgcgtcggg cagcggcgtg
     4021 cgcgccgtgc ccatgttgtc gtccatgtgc gccggctggc gcgtggccgg gatggcgcag
     4081 gcgacgaccg ggtgcgagac cacccaggcg aggaggaagc gcgcccaggt gtcgcagccg
     4141 agctcggggg cccaagcagg cagcggcgtg cggccgatgc ggtcgaagag cgcgccgccg
     4201 tcgaacggcc ggttgaccac caccgccatg ccgcgctcgg cggcgagctg catgagcggc
     4261 tcggcgctcg cgtccgccag gttgtaggtg atctgcagca cgtcgagcgg ctcgctgcgc
     4321 acgatgcgcg cggcctcgtc gtggcggctg ccgtgcgagg tggacacgcc gatggcacgc
     4381 gcgcggcccc cctccttcca gcggcgcagc agccgcaggt gggcgcgcca ctccagcagg
     4441 ttgtgcacca gcacgacgtc gaagcgcggc acgcgccaca gcgcgagcga atgctcgagc
     4501 tggcgcggcc cgagccgctc gaacggcgtc cacaccttgg tggcggcgac gagccggccc
     4561 gcgtgcggca ggcccggcag cagctcgccc agcaccgcct cggcgcggcc gtacatcggc
     4621 gacgagtcga tcatgccgcc gcccgcggcg aagaagcggc gcaggacctc gcggcggcgc
     4681 gcgagctcgc cggcgtcgtc gccgacgtcg aacgtgccca tgccgacgac cgggatcgcg
     4741 ccgagcgggc ccgcccggcg gcgcagcatg ggcgcggcgc cgggggcggg g
//
LOCUS       DASRIE010000168         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2788035, whole genome shotgun sequence.
ACCESSION   DASRIE010000168 DASRIE010000000
VERSION     DASRIE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788035"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFB53_07220"
     CDS             <1..557
                     /locus_tag="VFB53_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN86558.1"
                     /translation="GDDAREIQRILPSLILERTPLWAQVVFFGALLSAILSTASGAII
                     APTSLFTENVIRPFVRNMSDRQFMLLLRVILVTFSLAALLFALNSKSTMYEMVQNAYK
                     VTLVSCIVPLAAGVFWKRANVPGAMLSVVFGLVSWTIAEWVAADATIPPQLVGLAFSI
                     VGMVLGSLVPRAAASPAIHHHGKH"
     gene            601..1626
                     /locus_tag="VFB53_07225"
     CDS             601..1626
                     /locus_tag="VFB53_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN86559.1"
                     /translation="MFLVYVLLVAYASLYPLSGWRDVGLSPFAYLTGPWPRYVTAFDI
                     AVNLAGYVPFGFLAVTALHPRVRGAAALLAAVAGAAVLSLALEAAQSYLPARVPTNQD
                     VLCNVAGGALGALLGGHFTPFIAGGPLSRRRIAAFEPGAGADFGLVLIALWLFIQLNP
                     VALLFGAGDLRDFLAPFEGRARRPEFFVAIEAFTTAANLVAVALILSALAAPGGRVRA
                     LVIAVVLAALAVKTAAVAILMRAEDVFGWLTHGAQIGLGVGIVVAAVAVALPRTARLV
                     LAAVLLMAATVLVNLTPPNPYLADSLKVWQQGHFLNFNGLTRLVSALWPFIALGYLIY
                     LASRRLK"
     gene            1631..1942
                     /locus_tag="VFB53_07230"
     CDS             1631..1942
                     /locus_tag="VFB53_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZN86560.1"
                     /translation="MSYYAKHVFFCCNQREGTDRPSCNAKGASKLRDYAKGRIKALGM
                     AGEGRVRINQAGCLDRCEEGPCIVVYPEGVWYTYVDQHDIDEIIEEHLQNGRIVERLR
                     M"
     gene            1943..>2106
                     /locus_tag="VFB53_07235"
     CDS             1943..>2106
                     /locus_tag="VFB53_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86561.1"
                     /translation="MRASTRREFVAGPAGRIECAVDQPEGTARGIALIAHPHPLFGGT
                     LDNKVVQTLAR"
BASE COUNT          259 a          772 c          706 g          369 t
ORIGIN      
        1 ccggcgacga cgcccgcgag atccagcgca tcctgcccag cctgatcctc gagcgcacgc
       61 cgttgtgggc ccaggtggtg tttttcggcg cgctgctctc cgcgatcctg tccaccgcga
      121 gcggcgcgat catcgccccg acctcgcttt tcaccgagaa cgtgatccgc cccttcgtgc
      181 gcaacatgag cgaccgccag ttcatgctgc tcctgcgggt gatcctggtg accttcagcc
      241 tcgcggcgct gctcttcgcg ctcaactcga agagcaccat gtacgagatg gtgcagaacg
      301 cctacaaggt gacgctcgtc tcctgcatcg tgccgctcgc cgcgggcgtg ttctggaagc
      361 gcgccaacgt gcccggcgcc atgctgtcgg tggtgttcgg gctggtgtcg tggaccatcg
      421 ccgagtgggt ggcggccgac gccaccatcc cgccgcagct cgtcggcctc gccttctcca
      481 tcgtgggcat ggtgctcggc tccctcgtgc cgcgcgccgc ggcctcgccg gcgatccacc
      541 accacggcaa gcactgagca agtcgtagcg tccgcgcgcg cctccgggct cgcgcgcatc
      601 ctgtttctcg tctatgtcct gctggtcgcg tacgcctcgc tctacccgct ttccggctgg
      661 cgcgacgtcg ggctttcgcc cttcgcctac ctgaccgggc catggccgcg ctacgtcacc
      721 gccttcgaca tcgcggtgaa cctcgcgggc tacgtgccgt tcggcttcct cgcggtcacc
      781 gcgctgcacc cgcgcgtgcg cggcgccgcc gcgctgctcg ccgcggtggc gggcgcggcc
      841 gtgctgtcgc tcgcgctcga ggcggcgcag agctacctgc cggcgcgcgt gccgaccaac
      901 caggacgtgc tgtgcaacgt ggccggcggc gcgctcggcg ccctgctcgg gggccacttc
      961 acgcctttca tcgccggcgg cccgctgtcg cgccgccgca tcgccgcgtt cgagcccggc
     1021 gcgggcgcgg atttcgggct ggtgctgatc gcgctgtggc tcttcatcca gctcaacccg
     1081 gtggcgctgc ttttcggcgc gggcgacctg cgcgacttcc tcgcgccgtt cgaggggcgc
     1141 gcgcggcgcc cggagttctt cgtcgcgatc gaggccttca ccaccgcggc gaacctggtc
     1201 gcggtcgcgc tcatcctctc ggcgctcgcc gcgcccggcg gccgggtgcg cgcgctggtg
     1261 atcgccgtgg tgctcgccgc gctcgcggtg aaaaccgcgg ccgtggccat cctgatgcgc
     1321 gccgaggatg tgttcggctg gctcacgcac ggcgcgcaga tcggcctggg ggtgggcatc
     1381 gtggtagcgg cggtggccgt cgcgctgccg cgcaccgcgc gcctcgtgct cgccgcggtg
     1441 ctgctcatgg cggcgacggt gctcgtcaac ctgacgccgc ccaaccctta cctcgccgat
     1501 tcgctcaagg tgtggcagca gggtcacttt ctcaacttca acggccttac acgcctggtg
     1561 agcgcgctgt ggcccttcat cgcgctgggg tatctgattt acctcgcatc gcggcggtta
     1621 aaatagggcg atgtcgtatt acgcgaagca cgtcttcttc tgttgcaacc agcgcgaagg
     1681 caccgaccgg ccctcctgca acgccaaggg ggcctcgaag ctgcgcgact acgccaaggg
     1741 gcgcatcaag gcgctcggca tggcgggcga gggccgcgtg cgcatcaacc aggccggctg
     1801 cctcgaccgc tgcgaggaag gcccctgcat cgtggtgtat cccgagggcg tctggtacac
     1861 ctacgtcgac cagcacgaca tcgacgagat catcgaggag cacctgcaga acgggcgcat
     1921 cgtggaacgc ctgcgcatgt agatgcgggc gagcacccgt cgcgagttcg tcgccggccc
     1981 ggccggccgt atcgaatgcg ccgtcgacca gcccgaggga acagcgcgcg gcatcgccct
     2041 gatcgcgcat ccgcatccgc tcttcggcgg cacgctcgac aacaaggtgg tgcagacgct
     2101 ggcgcg
//
LOCUS       DASRIE010000169         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3840481, whole genome shotgun sequence.
ACCESSION   DASRIE010000169 DASRIE010000000
VERSION     DASRIE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840481"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFB53_07240"
     CDS             <1..594
                     /locus_tag="VFB53_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN86562.1"
                     /translation="LLYANGITPTVLDHDADQIETMRRFGWRVFYGDATRLDLLRTAG
                     AQKARVLVLAVDDIDQSVAIARMVREHFPALTVVARARNVDHLYALRDAGVALVERET
                     LDSALMSGRSVLESLGWQPHLARNLALRFRRHNVAQVASMAPHWKDEARRIAAAKQGR
                     RQLEEQFAQEREEAQRRQARAAWSERMASEDDDDDAA"
     gene            complement(614..1369)
                     /locus_tag="VFB53_07245"
     CDS             complement(614..1369)
                     /locus_tag="VFB53_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN86563.1"
                     /translation="MTLPVVAEAGFYAIAVPAVLLMGLAKSGFLGGFGSLAVPLMSLA
                     VPVPQAAAIMLPLLFVMDAVGLQQLWKQRDRELLRLLLPAGLLGTVVGTASFGLLPAR
                     TVAGVVGALTLLFLAQRLVFPPRADAPPPSKRLGFALGIASGFTSFVAHAGSPPIAAY
                     VLPLRLPPMTFAGTMAVFFAVVNLSKWIPYAWLGLIDARNMSTSLVLLPLAPLGVWLG
                     VWLTRRIASTWFYRLAYTGMFLTGAKLLWDGVA"
     gene            1462..1905
                     /locus_tag="VFB53_07250"
     CDS             1462..1905
                     /locus_tag="VFB53_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HZN86564.1"
                     /translation="MFAPSQNDVRRFFCDALRKQRDGLPLTPLETLAADWIAQHPEYH
                     GELADADAALAAVYDVEAGRTNPFLHLSMHLSISEQVGIDQPRGIRQAVELLAARRGS
                     LHAAQHEVMECLGEMMWASQRSGRPPDGEKYIDCVRRRATRDPAR"
     gene            complement(1902..2726)
                     /gene="panC"
                     /locus_tag="VFB53_07255"
     CDS             complement(1902..2726)
                     /gene="panC"
                     /locus_tag="VFB53_07255"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZN86565.1"
                     /translation="MRTVHTIADLRAAVAGGGCAFVPTMGNLHDGHLALVRQAGAHGL
                     PVVASIFVNRLQFAPHEDFDRYPRTLEHDSALLAGAGCDIVFAPAERELYPQPQTFKV
                     QPDPALADILEGHFRPGFFTGVCTVVMKLFTVVQPRVAVFGKKDYQQLMVIRRMVEQF
                     ALPVAVEAGETRREADGLALSSRNGYLSAAERAQAPELAAALRALADGVRAAPERRET
                     LEAQALAALAAQGWQPDYLAVRRRADLLAPAAGDRELVALGAARLGTTRLIDNLEF"
     gene            complement(2752..>3496)
                     /locus_tag="VFB53_07260"
     CDS             complement(2752..>3496)
                     /locus_tag="VFB53_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZN86566.1"
                     /translation="LQIPQDLYIPPDALEVILDAFEGPLDLLLYLIRRQNFNILDIPL
                     ADVTRQYLAYVDQIRKTHLELASEYLLMAAMLIEIKSRMLLPPKKSADGHEAEDPRAE
                     LVRRLIEYEQIKLAAARLEALPVLGRDFLRAQVVIEQALAPRFPDVDPDELRAAWADI
                     LKRARLNQHHTISREQLSVREHMSLVLRTLQGRRFIEFHELFDAARGTPVVIVTFIAL
                     LELAREHLLEITQAEAFAPIYVRLAYQPS"
BASE COUNT          527 a         1264 c         1279 g          426 t
ORIGIN      
        1 ctgctgtacg ccaacggcat cacgcccacc gtgctggacc acgacgccga ccagatcgag
       61 accatgcggc gcttcggctg gcgcgtgttc tacggcgacg ccacgcggct cgacctgctg
      121 cgcaccgcgg gcgcgcagaa ggcgcgcgtg ctggtgctcg ccgtggacga catcgaccag
      181 agcgtcgcca tcgcgcgcat ggtgcgcgag cacttcccgg cgctcaccgt cgtcgcgcgg
      241 gcgcgcaacg tcgaccacct ctacgcgctg cgcgacgcgg gcgtggcgct ggtcgagcgc
      301 gagacgctcg actccgcgct gatgagcggg cgcagcgtgc tggagtcgct cggctggcag
      361 ccgcacctcg cacgcaacct ggcgctgcgc tttcgccgcc acaacgtggc gcaggtggcg
      421 tcgatggcgc cgcactggaa ggacgaggcc aggcgcatcg cggccgccaa gcaggggcgg
      481 cggcagctcg aggagcagtt cgcgcaggag cgcgaggagg cgcagcggcg gcaggcgcgc
      541 gcggcatggt ccgagcgcat ggcgtccgag gacgacgacg acgacgcggc ctgacgaccg
      601 gcccgcgcgg gcctcaggcc acgccgtccc acagcagctt cgctcccgtg aggaacatcc
      661 cggtgtacgc gaggcggtag aaccacgtcg aggcgatgcg ccgggtgagc cacacgccga
      721 gccacacgcc gagcggcgcg agcggcagca gcaccagcga ggtcgacatg ttgcgcgcgt
      781 cgatcagccc gagccacgcg tacgggatcc acttggacag gttcacgacg gcgaagaaca
      841 ccgccatcgt gcccgcgaag gtcatgggcg gcaggcgcag cggcagcacg tacgccgcga
      901 tgggcgggct gccggcatgc gccacgaagc tggtgaagcc cgaggcgatc ccgagcgcga
      961 agcccagccg cttcgacggc ggcggcgcgt ccgcgcgcgg cgggaagacg agccgctgcg
     1021 cgaggaacag cagcgtgagc gcccccacca cgccggccac cgtgcgcgcc ggcagcagcc
     1081 cgaacgacgc ggtgccgacc accgtgccga gcagccccgc cggcagcagc agccgcagca
     1141 gctcgcggtc gcgctgcttc cacagctgct gcaggcccac cgcgtccatc acgaacagca
     1201 gcggcagcat gatcgcggcc gcctgcggca ctggcacggc gagcgacatc agcggcaccg
     1261 cgagcgagcc gaagccgccg aggaagccgc tcttggccag ccccatgagc agcaccgcgg
     1321 gcaccgcgat ggcgtagaag cccgcttccg cgacgaccgg cagcgtcatc ggcgccccct
     1381 cgccggccgc gcggtcccgc cgggcgggtc gggcgtcacc gccggcccgg ccgtgcggcg
     1441 gcgctcggcg acaatccccg catgttcgcg ccttcccaga acgacgtccg ccgcttcttc
     1501 tgcgacgcct tgcgcaagca gcgcgacggc ctgccgctca ccccgctgga aacgctcgcc
     1561 gccgactgga tcgcccagca ccccgaatac cacggcgagc tggccgacgc cgacgccgcg
     1621 ctggccgccg tgtacgacgt ggaagcgggc cgcaccaatc cgttccttca tctgtcgatg
     1681 cacctgtcga tcagcgagca ggtggggatc gaccagccgc gcggcatccg ccaggcggtt
     1741 gagctgctcg ccgcccggcg cggctcgctg catgccgcgc agcacgaggt gatggagtgc
     1801 ctcggcgaga tgatgtgggc gtcgcagcgc agcggccggc cgcccgacgg ggagaagtac
     1861 atcgactgcg tgcggcggcg ggccacgcgg gacccggcgc gctagaactc gaggttgtcg
     1921 atcaggcgcg tcgtgcccag gcgcgccgcg cccagcgcca ccagctcgcg atcgcccgcg
     1981 gccggcgcca gcaggtcggc gcggcggcgc accgcgaggt agtcgggctg ccagccctgc
     2041 gccgcgagcg cggccagcgc ctgcgcttcc agcgtttcgc ggcgttcggg cgcggcgcgc
     2101 acgccatcgg cgagcgcgcg cagggccgcc gccagctcgg gcgcctgcgc gcgctcggcc
     2161 gcgctcaggt agccgttgcg cgacgagagc gccaggccgt cggcctcgcg ccgcgtctcg
     2221 cccgcctcga cggcgaccgg cagggcgaac tgctccacca tgcggcggat gaccatgagc
     2281 tgctggtagt ccttcttgcc gaacaccgcc acgcgcggct gcaccaccgt gaacagcttc
     2341 atcaccaccg tgcagacccc ggtgaagaac ccggggcgga agtgaccttc gaggatgtcg
     2401 gccagcgccg ggtccggctg caccttgaag gtctgcggct gcgggtacag ctcgcgctcc
     2461 gcgggcgcga agacgatgtc gcagccggcg ccggccagca gcgcgctgtc gtgctcgagc
     2521 gtgcgcgggt agcgatcgaa gtcctcgtgc ggcgcgaact gcaggcggtt cacgaagatg
     2581 ctcgcgacca cgggcaggcc gtgcgcgccc gcctgccgca cgagcgcgag gtgcccgtcg
     2641 tgcaggttgc ccatggtggg cacgaaggcg cagccgccgc ccgcgacggc ggcgcgcagg
     2701 tcggcgatgg tgtggacggt gcgcatcgcg gcgctctcct gaaggcaaag gctaggacgg
     2761 ctggtacgcc agccggacgt agatcggcgc gaacgcctcg gcctgggtga tctccagcag
     2821 gtgctcgcgc gccagctcga gcagcgcgat gaaggtgacg atgaccaccg gcgtgccgcg
     2881 cgccgcgtcg aacagctcgt ggaactcgat gaagcgccgg ccctgcagcg tgcgcagcac
     2941 caggctcatg tgctcgcgca ccgagagctg ctcgcgcgag atggtgtggt gctggttcag
     3001 ccgggcgcgc ttgaggatgt cggcccacgc ggcgcgcagc tcgtcggggt ccacgtccgg
     3061 gaagcgcggc gcgagcgcct gctcgatcac cacctgcgcg cgcaggaagt cgcgccccag
     3121 caccggcagc gcctccaggc gcgcggcggc gagcttgatc tgctcgtact cgatcagccg
     3181 gcgcaccagc tcggcccgcg gatcctcggc ttcgtgcccg tcggccgact tcttcggcgg
     3241 cagcagcatg cgcgacttga tctcgatgag catcgccgcc atgagcaggt actcgctggc
     3301 gagctcgaga tgggtcttgc ggatctggtc gacgtaggcc aggtactggc gcgtcacgtc
     3361 ggccagcgga atgtccagga tgttgaagtt ctgccggcgg atcaggtaca gcagcaggtc
     3421 gagcggaccc tcgaaggcat cgaggatgac ctccagcgcg tccggcggga tgtacagatc
     3481 ctgtgggatc tgcagg
//
LOCUS       DASRIE010000170         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1225733, whole genome shotgun sequence.
ACCESSION   DASRIE010000170 DASRIE010000000
VERSION     DASRIE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225733"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFB53_07265"
     CDS             <1..623
                     /locus_tag="VFB53_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN86567.1"
                     /translation="RLIERTSGEVIFEGTDVMSLSGDALIQFRQRAQIVFQDPYASLN
                     PRKTIERAIGEPLRVHRGMNREQTRARVQELLQLVGLSPEHASRYPHQFSGGQRQRIC
                     IARALAMEPELLIADEAVSALDVSVQAQVLSLLEEIRERLKLAMLFITHDLRVAAQIC
                     DHVAVMSQGRVVEYGPAAQVFGAPRHAYTRALFDAAPGRDFAFGAA"
     gene            641..1399
                     /locus_tag="VFB53_07270"
     CDS             641..1399
                     /locus_tag="VFB53_07270"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZN86568.1"
                     /translation="MDQLDSSAIRVPGAEVRVVRRCTSTNTLLLALGAGQPVLLAADE
                     QSAGRGRRGRRWHSPAGSGVLFSLALPMRRPVRELGGLSIVAGLAAVRALRALGAAEA
                     ALKWPNDLLVRGAKLGGILVETRARDAGSLAVVGIGINHLPASGIAARLRRSVAALAG
                     LLRPLPARNAVIGALGRELFAALRAFDATGFEPFRAAWEGAHAHAGQRLRVRLADGRV
                     VAGMAAGLAADGALQLRTQRGLRTVRNGRVVSAA"
     gene            1396..2118
                     /locus_tag="VFB53_07275"
     CDS             1396..2118
                     /locus_tag="VFB53_07275"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZN86569.1"
                     /translation="MILAIDAGNSRVKWGWHDRRDWHNLATVSLIEFAAANHDINPFA
                     ATHENPQRIVISNVAGEGAHQLLINWTSIFDAEPLWLKGEAERCGVRSLYQRPEQLGP
                     DRWAALVAARALHRGPCLVVNAGTATTADVLSSGGEFLGGLILPGVDLMRFVLHEHTG
                     RLPLQEGRFAPTPRNTVDAIETGCRHAQAGAVERMFRVAGPGAQCLVSGGAGKALIEQ
                     LDIPCRYVENLVLEGLARIAWS"
     gene            2115..2798
                     /locus_tag="VFB53_07280"
     CDS             2115..2798
                     /locus_tag="VFB53_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZN86570.1"
                     /translation="MRLLFLLLLAANLAFFAWMRFLALPDPAVDRQPQSQQLDPGKLR
                     IVPASELARTPPPAAAPAKPKPAPPPPPPIACLEWGSFSPADASRAAQRLEPLSLGAR
                     LAQVRGEEIAGWWVHIPPQGNRAGALRKAAELKKLGVPDYFVVQEEGRWRWALSLGVF
                     RAEDAAKSHLEALRAKGVRSAVIGQRETRVPKVWFQVRAVEPALQQRLQELAREFEGA
                     ALQACVQRG"
     gene            complement(2802..3182)
                     /locus_tag="VFB53_07285"
     CDS             complement(2802..3182)
                     /locus_tag="VFB53_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86571.1"
                     /translation="MAARILIVDDDESLRELLRLHLAAAGYEVQVAPDAIAAGYLVLQ
                     GAPDLIITDVNMPHMDGFEFVAALRADKALPHIPVIFLTSVEEGEQRGKELGAVGYVT
                     KPVRADRLLALVAEHVPGGTFPIG"
     gene            complement(3201..3273)
                     /locus_tag="VFB53_07290"
     tRNA            complement(3201..3273)
                     /locus_tag="VFB53_07290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3238..3240),aa:Thr,seq:cgt)
     gene            3466..3885
                     /locus_tag="VFB53_07295"
     CDS             3466..3885
                     /locus_tag="VFB53_07295"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HZN86572.1"
                     /translation="MQSNQRGMTIVELLVVMTIVGILAAVAFPAYEGQQRKGTRAAAQ
                     AAMVNIANKQAQILLDARNYALGAGALAALNVTLPVEVTSFYTVTVTDKDGATAGTNP
                     PTFTITATPIAGKRQVADGVLTLNHTGAKTRDGKTGW"
     gene            3888..4337
                     /locus_tag="VFB53_07300"
     CDS             3888..4337
                     /locus_tag="VFB53_07300"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZN86573.1"
                     /translation="MRTAAGFTLPELLIVMTVLAILVAAGLPSFGEFVRNQRLKTASF
                     DLFSTLVVARSEAINRNASVTLAPVVAGNWSGGWTVTAAGGTVIRSQEAIPDITIAGP
                     DNLVYSGSGRLSGAVAQFSLTASGSNVVNRCISIDLSGRPVSKAAAC"
     gene            4331..4915
                     /gene="pilV"
                     /locus_tag="VFB53_07305"
     CDS             4331..4915
                     /gene="pilV"
                     /locus_tag="VFB53_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HZN86574.1"
                     /translation="MLSRGSLRRSRGVTMLEVLIAIVILTIGLLGVAGMQAQMQNAEV
                     EAYQRAQAIVLLQDMVDRINANRRNAANYVTGTPLGTASDIDCSAPATVAERDQCAWD
                     ASLRGAAETAGGAQLGAMNGARGCISNPVAVMPRQVIVAVAWQGNSPTAAPGGTNCGQ
                     GEFGADDKFRRAMVASVVIGCLQNDPATGNCVTP"
     gene            4912..5928
                     /locus_tag="VFB53_07310"
     CDS             4912..5928
                     /locus_tag="VFB53_07310"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HZN86575.1"
                     /translation="MTARRQTGLSLIELMISLTLGLLVLSGVLMVFVNTSASRNEVER
                     TSRQIENGRFAVELVSTDLRLAGFYGELDVSSVTPPAAVPANPCSLAAADWNAWIPVH
                     LQGFDNAGFVSADCPFDGLKAGTDVLVVRRARACAAGVAGCDAAINDEAYVQAGLCGT
                     VTTTHRLGVRGVQAFDLEKKDCATAADLRQYLVHVYYVSTNNGAGVNVPTLKRFELKL
                     GEFVSTPLVEGIEEFQVEYGLDTDGDGVPDSYVANPSNFPKGACAGACPLNNWMNVVT
                     ARVHLLARNLETSPGFTDAKTYALGNDIDGAPVTVTPGGAYRRHVYSSLVRVANAAGR
                     RDKP"
     gene            5925..>6187
                     /locus_tag="VFB53_07315"
     CDS             5925..>6187
                     /locus_tag="VFB53_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HZN86576.1"
                     /translation="MMRSAIRQRGATLVIALIMLVLLTLFAVSSMNTANTNLRVVGNM
                     QSRNEALYAAQETLETVISTPLFLANPANAVLNPCGAQNTMCN"
BASE COUNT          924 a         2203 c         2193 g          867 t
ORIGIN      
        1 tgcggctgat cgagcggaca tcgggcgagg tgatcttcga gggcaccgac gtcatgtcgc
       61 tcagcggcga cgcgctgatc cagttccgtc aacgcgccca gatcgtgttc caggacccgt
      121 acgcgtcgct caacccacgc aagacgatcg agcgggcgat cggcgagcct ctgcgcgtcc
      181 accgcggcat gaaccgcgag cagacgcggg cgcgggtgca ggagctgctg cagctcgtcg
      241 gcctgtcgcc ggagcatgcc agccgctacc cgcaccagtt ctccggcggc cagcgccagc
      301 gcatctgcat cgcgcgcgcg ctcgccatgg agcccgagct gctgatcgcc gacgaggcgg
      361 tgtccgcgct cgacgtctcg gtgcaggcgc aggtgctttc gctgctggag gaaatccgcg
      421 agcgcctgaa gctggccatg ctgttcatca cccacgacct gcgcgtcgcg gcgcagatct
      481 gcgaccacgt ggcggtgatg tcgcaggggc gcgtggtcga gtacggcccg gcggcgcagg
      541 tgttcggcgc cccgcggcac gcctacacgc gcgcgctgtt cgacgcggcg ccggggcgcg
      601 atttcgcgtt cggcgccgcg taaaccgtaa actacggccg atggaccagc tcgacagcag
      661 cgcgatccgc gtcccgggcg ccgaggtgcg cgtggtgcgg cgctgcacct cgaccaacac
      721 gctgctgctg gcgctcggcg ccgggcagcc ggtgctgctc gccgccgacg agcagagcgc
      781 cggccgcggc aggcgcggca ggcgctggca cagcccggcg ggcagcggcg tgctgttctc
      841 tctcgccctg ccgatgcgcc ggccggtgcg cgagctcggc gggctgtcga tcgtcgccgg
      901 cctcgccgcg gtgcgcgcgc tgcgcgcgct cggcgccgcc gaggcggcgc tgaagtggcc
      961 caacgacctg ctggtgcgcg gggccaagct cggcggcatc ctggtggaga cgcgcgcgcg
     1021 cgacgccggc agcctggcgg tggtcggcat cggcatcaac cacctgccgg cgtccgggat
     1081 tgcggcccgc ctgcggcgca gcgtcgccgc gctcgccggg ctgctgcgcc cgctgccggc
     1141 gcgcaacgcc gtcatcggcg cgctgggacg cgagctgttc gccgcgctgc gcgccttcga
     1201 cgccaccggc ttcgagccct tccgcgccgc ttgggagggc gcgcacgccc acgccgggca
     1261 gcgcctgcgc gtgcgcctgg ccgacgggcg cgtggtggcc ggcatggccg cgggactggc
     1321 cgccgacggc gccctgcagc tgcgcacgca gcgcgggctg cgcacggtgc gcaacggccg
     1381 cgtggtgtcg gcggcatgat cctcgccatc gacgccggca attcccgcgt caaatggggc
     1441 tggcacgacc gccgcgactg gcacaacctc gccaccgtgt cgctcatcga gttcgccgcg
     1501 gccaaccacg acatcaaccc cttcgccgcg acgcacgaga acccgcagcg catcgtcatc
     1561 tccaacgtcg ccggcgaggg cgcgcaccag ctgctgatca actggacgag catctttgac
     1621 gccgagccgc tgtggctcaa gggggaagcc gagcgctgcg gcgtgcgcag cctgtaccag
     1681 cgtcccgaac agctggggcc ggatcgctgg gcggcgctgg tggccgcgcg ggccctgcat
     1741 cggggaccct gcctggtcgt caatgcgggc accgcgacca ccgccgacgt gctgtcgtcc
     1801 ggcggcgagt ttctcggcgg cctgatcctg cccggggtgg acctgatgcg cttcgtcctg
     1861 catgagcaca ccgggcgcct gccgctgcag gaaggccgct tcgcgccgac gccgcgcaac
     1921 acggtggacg ccatcgagac cggctgccgc cacgcgcagg cgggcgcggt cgaacgcatg
     1981 ttccgcgtcg cggggccggg cgcgcagtgc ctggtgtcgg gcggcgccgg caaggcgctc
     2041 atcgagcagc tggacattcc ctgccgctac gtggaaaacc tggtgctgga aggactggcg
     2101 cggatcgcct ggtcatgagg ctgctgttcc tgctgctgct ggccgccaac ctcgcgtttt
     2161 tcgcctggat gcgcttcctc gccctgcccg acccggcggt cgaccgccag ccccagtcgc
     2221 agcagctcga cccgggcaag ctgcgcatcg tgccggccag cgaactcgcc aggacgccgc
     2281 cccccgccgc cgccccggcc aaaccgaagc cggccccgcc gcccccgccg ccgatcgcct
     2341 gcctcgagtg gggcagcttc agcccggccg acgcgtcgcg tgccgcccag cgcctcgagc
     2401 cgctctccct cggcgcccgg ctggcgcagg tgcgcggcga ggagatcgcc ggctggtggg
     2461 tccatatccc gccgcaaggc aaccgggccg gggcgctgcg caaggcggcc gagttgaaga
     2521 agctgggggt gccggattat ttcgtggtgc aggaggaagg ccgctggcgc tgggcgctgt
     2581 cgctcggcgt gttccgcgcc gaagacgcgg ccaagtccca cctggaagcg ctgcgcgcca
     2641 agggcgtcag gagcgcggtg atcggccagc gcgagacccg cgtgccgaag gtctggttcc
     2701 aggtgcgcgc cgtcgagccg gccctgcagc agcgcctgca ggagctggcg cgcgaattcg
     2761 agggcgccgc gctgcaggcc tgcgtgcagc gcggctgaag gctagccgat gggaaacgtg
     2821 ccgcccggca cgtgctcggc cacgagcgcc agcagccggt cggcgcgcac cggcttggtg
     2881 acgtagccca ccgcgccgag ctccttgccg cgctgctcgc cctcctcgac cgaagtgagg
     2941 aagatcaccg ggatgtgcgg cagcgccttg tcggcgcgca gcgccgcgac gaactcgaag
     3001 ccgtccatgt gcggcatgtt gacgtcggtg atgatgagat cgggggcgcc ttgcagcacc
     3061 aggtagccgg cggcgatggc gtcgggggcc acctgcacct cgtagccggc ggcagccagg
     3121 tgcaggcgca gcagctcgcg caggctctcg tcgtcgtcca cgatcaggat gcgtgcagcc
     3181 atcggcgagt gtcgtctcgg tgctggtggc ccgactcgaa cgggcgacct actgattacg
     3241 aatcagttgc tctaccagct gagctacacc agcggggaat tggtgctgca aaagggcgca
     3301 attatacgcc gggccgccga gtgtgacgct tttggcagga acgcgcgccc cggctagcat
     3361 gcaccggggg cgaaatgccg ccggtccgcg gtcgccgacc actcgtcgcg ccggctggcg
     3421 gcaggcgccg gtccgagaat attcttccgg gggagggaaa tgcacatgca atccaatcaa
     3481 cgaggcatga ccatcgtcga gctgctcgtg gtgatgacga tcgtcggcat ccttgccgcg
     3541 gtcgcctttc cggcctacga gggccagcag cgcaagggca cgcgcgcggc agcgcaggcg
     3601 gcgatggtca acatcgcgaa caagcaggcg cagatcctgc tcgacgcgcg caattatgcc
     3661 cttggcgcgg gcgcattggc cgcgctgaac gtcacgctgc cggtggaggt cacctcgttc
     3721 tacacggtga ccgtgacgga caaagacggc gccacggccg ggaccaaccc ccctaccttc
     3781 actatcactg ccacgccgat cgcgggcaag cggcaggtcg ccgacggggt cctcacgctg
     3841 aaccacaccg gcgcgaaaac gcgcgacggc aagaccggat ggtaaggatg cgcaccgcgg
     3901 cgggattcac gctgcccgag ctgctgatcg tgatgacggt gctcgcgatc ctggtcgcgg
     3961 ccggcctgcc gagcttcggc gagttcgtgc gcaaccagcg cctcaagacc gccagcttcg
     4021 acctgttttc caccctggtg gtggcgcgca gcgaggcgat caaccgcaac gcgagcgtca
     4081 cgctggctcc ggtggtggcc ggcaactgga gtggcggctg gaccgtcacc gcggccggcg
     4141 gcacggtgat ccgctcgcag gaagccatcc ccgacatcac catcgccggc ccggacaacc
     4201 tggtctacag cggcagcggc aggctgagcg gcgccgtggc gcagttctcg ctgacggcgt
     4261 ccggcagcaa cgtggtcaac cgttgcatct ccatcgacct gagcggacgg cccgtgtcga
     4321 aggcggccgc atgctgagcc gcggcagcct gcggcgcagc cgcggcgtga ccatgctgga
     4381 ggtgctgatt gccatcgtga tcctcaccat cggcctgctc ggcgtggccg gcatgcaggc
     4441 gcagatgcag aacgccgagg tcgaggccta ccagcgggcc caggcgatcg tgctgctgca
     4501 ggacatggtc gaccgcatca acgcgaaccg caggaacgcg gcgaactacg tcaccggcac
     4561 cccgctcggc accgcaagcg acatcgactg cagcgcgccg gcgaccgtgg cggaaaggga
     4621 ccagtgcgcc tgggacgcgt cgctgcgcgg cgccgcggaa accgccggcg gcgcgcagct
     4681 cggcgcgatg aacggggcgc gcggctgcat cagcaacccg gtggccgtca tgccgcgcca
     4741 ggtgatcgtc gccgtggcct ggcagggcaa ctcgccgacc gccgcgcccg gagggaccaa
     4801 ttgcggccag ggcgagttcg gcgccgacga caagttccgg cgcgccatgg tggcgagcgt
     4861 ggtgatcggc tgcctgcaga acgacccggc gacgggcaac tgcgtgaccc catgaccgcg
     4921 cgccgccaga caggcctgtc gctcatcgag ctgatgattt cgctcaccct cgggctgctg
     4981 gtgctctcgg gcgtgttgat ggtgttcgtc aataccagcg cctcgcgcaa cgaggtcgag
     5041 cgcaccagcc gccagatcga gaacggccgc ttcgccgtgg agctcgtctc caccgacctg
     5101 cgcctcgccg ggttctacgg ggaactggac gtcagctcgg tgacgccccc cgccgccgtg
     5161 cccgccaacc cctgttcgct ggccgccgcg gactggaacg cctggatccc ggtccacctg
     5221 cagggcttcg acaacgccgg attcgtgtcg gcggactgcc cgttcgacgg tctcaaggcc
     5281 ggcaccgacg tgctggtggt gcgccgcgcg cgcgcctgcg ccgccggcgt cgccggatgc
     5341 gatgcggcga tcaacgacga ggcgtacgtg caggcgggtc tgtgcgggac ggtgaccacc
     5401 acgcacaggc tcggcgtgcg cggcgtccag gccttcgacc tggaaaagaa ggattgcgcg
     5461 acggccgcgg acctgcgcca gtacctggtg cacgtctact atgtcagcac gaacaacggc
     5521 gcgggcgtca acgtgccgac gctcaagcgc ttcgagctga agctgggcga attcgtgagc
     5581 acgccgctgg tcgagggcat cgaagagttc caggtcgaat acggcctgga caccgacggc
     5641 gacggggtgc cggactccta tgtcgccaat ccgagcaatt tccccaaggg ggcctgcgcg
     5701 ggcgcctgcc cgctcaacaa ctggatgaac gtggtcaccg cgcgcgtcca cctgctggcg
     5761 cgcaatctgg aaacctcccc tggcttcacc gacgccaaga cctacgcgct cggcaacgat
     5821 atcgacggcg ccccggtcac ggtgaccccc ggcggcgcct accgccggca cgtctacagc
     5881 agcctggtgc gcgtcgccaa cgccgcgggc aggagggaca agccatgatg cgctctgcaa
     5941 tccggcaacg cggcgccacg ctggtgatcg cgctgatcat gctggtgctg ctcaccctgt
     6001 tcgcggtgag ctcgatgaac accgccaaca ccaatctgcg cgtggtcggc aacatgcagt
     6061 ccaggaacga ggcgctgtac gcggcgcagg agacgcttga aaccgtgatc agcacgccgc
     6121 tgttcctcgc caacccggcg aacgctgtgc tcaacccctg cggcgcgcag aacaccatgt
     6181 gcaacga
//
LOCUS       DASRIE010000171         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1730738, whole genome shotgun sequence.
ACCESSION   DASRIE010000171 DASRIE010000000
VERSION     DASRIE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730738"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..916)
                     /locus_tag="VFB53_07320"
     CDS             complement(104..916)
                     /locus_tag="VFB53_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZN86577.1"
                     /translation="MNDVLLRMDLGAWKPVLGALVVPPVPLLLLVVAGALGVARRRAW
                     GALLVALGVAGLWFGATGVASDWLERRLLKPPPALPAARIEALRRDASARGTGAIVVL
                     GGGMDALAPEYGTSSLSDASLQRLRYGLWLGRQTGLPVGFSGGTGWGGDAGTSEAEVA
                     SRIAAQEFGRPLRWTEGASRDTRENAARMVPLLQRAGITHVVLVTHGWHMPRALAQFE
                     RAAAPAGIRIEPAPMGLAKRSGRGAIDWLPSAHGFARMRHVVHEWVARTAGH"
     gene            973..1476
                     /locus_tag="VFB53_07325"
     CDS             973..1476
                     /locus_tag="VFB53_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN86578.1"
                     /translation="MDEDRRAALRSRLIRNIDEVERVRETRDPLYDTLTARLATGTAA
                     SKLGISVDTLAPGRRGCPYHLHHAQEEAFVILRGHGSLRVAGELLPLRAGDVVFIPAG
                     PEYPHQIVNTSDAPLEYLSISTMDSPEICEYPDSRKVYATARHAGGRFAHLHRTGDDL
                     DYWDAEP"
     gene            complement(1545..2741)
                     /locus_tag="VFB53_07330"
     CDS             complement(1545..2741)
                     /locus_tag="VFB53_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN86579.1"
                     /translation="MKAAFQWDDPFRLDDQLSEDERAVRDAAREYAQGRLQPRVLMAA
                     RHETFDREIMNEMGALGLLGSTIEGYGCPGVNHVSYGLVAREVERVDSGYRSAMSVQS
                     SLVMHPIHAYGNEAQRQKYLPKLATGEWVGCFGLTEPNHGSDPGSMESRARAVDGGYV
                     LKGNKMWITNSPIADVFVVWAKLVDADGRAGGQEAIRGFVLDKGMKGLSAPKIEGKMS
                     LRASITGEIVMDDVFVPAENLLPDVRGLKGPFGCLNKARYGIAWGALGAAEFCWHAAR
                     QYTLDRKQFGRPLAATQLVQRKLADMQTEITLGLQGCLRVGRLMDEGQAAPEMVSLIK
                     RNSCGKSLEIARVARDMHGGNGIHDEFHVIRHMINLETVNTYEGTHDVHALILGRAQT
                     GLQAFG"
     gene            2840..3382
                     /gene="msrA"
                     /locus_tag="VFB53_07335"
     CDS             2840..3382
                     /gene="msrA"
                     /locus_tag="VFB53_07335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZN86580.1"
                     /translation="MNTTTIDTITLGGGCFWCLEAVYERVDGVTAVESGYANGHVKNP
                     SYEQVCRGDTGHAEVVRVSFDTARIGLREILEIFFTIHDPTTPNRQGNDAGPQYRSGI
                     YVHHPEQERVAREVLAEVNQHHGGRVVTELAPVANYTRAEEHHQHYFARNPSQGYCAF
                     VVAPKVEKFRRTFAHRVRGA"
BASE COUNT          498 a         1255 c         1194 g          486 t
ORIGIN      
        1 cccttcgaca agctcaggac aggcccttgc gaggagcccg gctttgccgg gcctcctcgc
       61 accgatcaaa aacccactga aaaaattgcg tccagcaatt ttttcagtgc ccggcggtcc
      121 gggccaccca ctcgtgaacc acgtgacgca tgcgcgcgaa gccgtgggcg ctcggcagcc
      181 agtcgatcgc gccgcgcccg gagcgcttcg ccaggcccat cggcgcgggc tcgatgcgga
      241 tgccggccgg cgccgccgcg cgctcgaact gcgcgagcgc gcgcggcatg tgccagccgt
      301 gcgtgaccag cacgacgtgc gtgattccgg cgcgctgcag cagcggcacc atgcgcgccg
      361 cgttctcgcg ggtgtcgcgc gaggcgccct cggtccagcg cagcggccgc ccgaactcct
      421 gcgccgcgat gcgcgatgcc acctccgcct cgctggtgcc cgcgtccccg ccccagccgg
      481 tgccgccgct gaagcccacc ggcagcccgg tctggcggcc gagccagagg ccgtagcgca
      541 gccgctgcag cgaggcgtcg ctcaggctgc tcgtgccgta ctcgggcgcc agcgcatcca
      601 tgccgccgcc cagcacgacg atcgcccccg tgcctcgagc gctcgcgtcg cgtcgcaggg
      661 cctcgatgcg cgccgccggc agcgcgggcg gcggcttcag cagccggcgc tccagccagt
      721 cgctcgcgac gccggtcgcg ccgaaccaga ggcccgccac gccgagcgcg accagcagcg
      781 cgccccaggc ccgccgccgg gcgacaccga gcgcgcccgc gacgacgagc agcagcagcg
      841 gcacgggcgg aaccaccagt gcgccgagga ccggtttcca ggccccgagg tccatgcgca
      901 ggaggacgtc gttcacggtt gtgcgaaggg cggcgggcgc tgcatgatgc ctgcctgccc
      961 cggaggacgc acatggacga agaccgccgc gccgcgttga ggtcccggct gatccgcaac
     1021 atcgacgagg tggagcgggt gcgcgagacg cgcgatccgc tgtacgacac gctcaccgcg
     1081 cgcctggcga ccggcacggc ggcgtcgaag ctcgggatca gcgtggacac gctggcgccg
     1141 ggccggcgcg gctgtcccta ccacctgcat cacgcgcagg aggaggcatt cgtcatcctg
     1201 cgcggccatg ggtcgctgcg cgtggcgggc gagctgctgc ccctgcgcgc cggcgacgtc
     1261 gtcttcatcc cggccgggcc ggagtacccg caccagatcg tcaacacctc cgacgcgccg
     1321 ctcgagtacc tgtcgatcag cacgatggac agcccggaga tctgcgagta cccggattcg
     1381 cgcaaggtct acgcgacggc gcggcatgcg ggcggccggt tcgcgcacct gcaccgcacg
     1441 ggcgacgacc tcgactactg ggacgcggag ccctgagccg gcgcagcgca gtcggcttgc
     1501 aggcgatccg acggtgcgca gccaaatgcc ccacgcgccg cgagtcagcc gaacgcctgc
     1561 aggccggtct gggcgcggcc caggatcagc gcgtgcacgt cgtgcgtgcc ctcgtacgtg
     1621 ttgaccgtct cgaggttgat catgtggcgg atgacgtgga actcgtcgtg gatgccgttg
     1681 ccgccgtgca tgtcgcgcgc cacgcgggcg atctcgagcg acttgccgca gctgttgcgc
     1741 ttgatgagcg acaccatctc gggggcggcc tgcccctcgt ccatcaggcg gcccacgcgc
     1801 aggcagccct gcaagccgag ggtgatctcg gtctgcatgt cggccagctt gcgctgcacg
     1861 agctgcgtgg cggccagcgg gcggccgaac tgcttgcggt ccagcgtgta ctgccgggcg
     1921 gcgtgccagc agaactcggc cgcgccgagc gcgccccagg cgatgccgta gcgtgccttg
     1981 ttgaggcagc cgaacgggcc cttcaggccc cgcacgtcgg gcagcaggtt ctccgccggc
     2041 acgaagacgt cgtccatcac gatctcgccg gtgatcgagg cgcgcaggct catcttgccc
     2101 tcgatcttcg gcgcggagag gcccttcatg cccttgtcga gcacgaagcc gcggatcgct
     2161 tcctggccgc cggcgcgccc gtcggcgtcc accagcttgg cccagaccac gaacacgtcg
     2221 gcgatcggcg agttggtgat ccacatcttg ttgcccttca gcacgtagcc gccgtcgacg
     2281 gcgcgcgcgc ggctctccat gctgcccggg tcgctgccgt ggttcggctc ggtgaggccg
     2341 aagcagccga cccattcgcc ggtcgcgagc ttcggcaggt acttctggcg ctgcgcctcg
     2401 ttgccgtagg cgtggatcgg gtgcatcacc agcgagctct gcacgctcat cgcgctgcgg
     2461 tagccgctgt cgacgcgctc cacctcgcgc gccaccagcc cgtagctcac gtggttcacg
     2521 ccgggacagc cgtagccttc gatggtggag ccgagcaggc cgagcgcgcc catctcgttc
     2581 atgatctcgc ggtcgaacgt ctcgtggcgc gcggccatca gcacgcgcgg ctgcaggcgg
     2641 ccctgggcgt attcgcgcgc cgcgtcgcgc accgcgcgct cgtcctcgct cagctggtcg
     2701 tcgagacgga agggatcatc ccattggaag gcggctttca tggcttcacg acctcgcgga
     2761 tacggatccc gtcgatgata cggggccgtc gtcgacgggg tcgtcggcgc ggcgatcggc
     2821 cttcaccgca ggagcgcgca tgaacaccac gaccatcgac accatcaccc tgggcggcgg
     2881 ctgcttctgg tgcctggaag cggtgtacga gcgcgtggac ggggtcaccg cggtcgaatc
     2941 cggctatgcc aacggccacg tgaagaaccc ctcctacgag caggtgtgcc gcggcgacac
     3001 cgggcatgcc gaagtggtgc gggtgagctt cgacaccgcg cgcatcgggc tgcgcgagat
     3061 cctcgagatc ttcttcacca tccacgaccc caccacgccg aaccggcagg gcaacgacgc
     3121 ggggccccag taccgctccg gcatctacgt ccaccatccc gagcaggaac gcgtggcgcg
     3181 cgaggtgctg gccgaggtga accagcacca cggcgggcgc gtggtcaccg agctggcgcc
     3241 ggtggcgaac tacacgcgcg cggaggagca tcaccagcac tacttcgcgc gcaacccgtc
     3301 gcaggggtat tgcgcgttcg tcgtcgcgcc gaaggtggag aagttccgca ggaccttcgc
     3361 gcatcgcgtg cgcggcgcgt gagcagcgcc gggccgctcc cgagcctgcc ctgagcgtgt
     3421 cgaaggggcg aac
//
LOCUS       DASRIE010000172         5071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2498964, whole genome shotgun sequence.
ACCESSION   DASRIE010000172 DASRIE010000000
VERSION     DASRIE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498964"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VFB53_07340"
     CDS             complement(<1..1201)
                     /locus_tag="VFB53_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN86581.1"
                     /translation="MLLTRKTSGAGVAAPSSSLVSSLARGVSRAIPTMDRRAFLRRSG
                     LGVGAGLAASQLTLVRKAHAADAPKSGGPRIEVKRTVCGHCSVGCAVDAVVQNGVWIR
                     QEPVFDSPINLGAHCAKGAALREHGHGEYRLKYPMKLVDGKYRRISWDQALNEISAKM
                     LELRKQSGPDSVFLVGSSKHSNEQAYLMRKWVSLWGSNNCDHQARICHSTTVAGVANT
                     WGYGAMTNSYNDMQNAKAALYIGSNAAEAHPVSMLHMLHAKETGTKMIVVDPRFTRTA
                     AKADHYIRIRSGTDIPFLFGMLHHIFKNGWEDKKYIADRVYGMDKIRDEVLAKWTPDK
                     VQEVCGVDEATCLNAAKIMAENRPSTIVWCMGQTQHTIGNAIVRASCIVQLALGNIGV
                     SGGGANIF"
     gene            complement(1234..1458)
                     /locus_tag="VFB53_07345"
     CDS             complement(1234..1458)
                     /locus_tag="VFB53_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HZN86582.1"
                     /translation="MKPTGPEAPGASPNQRRSLLLGAGAAGAAVLAARAWPTAPADAA
                     APGAAAPEAAAKGGYQLTQHVLRYYETTKV"
     gene            complement(1596..2225)
                     /locus_tag="VFB53_07350"
     CDS             complement(1596..2225)
                     /locus_tag="VFB53_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HZN86583.1"
                     /translation="MTTRPLRFEPAPDDDREELARAESYGLLAALFYAPPPPQLHAQL
                     RVAPTVAPAPGGLLEGSWSEVVAAARRRTVEEIAGEYAALFLGLGKPEVFLYGSHHLA
                     GALNETPLVALRETLARLGLERVEGMYETEDHVAYLCEAMRFLIAGDDPAVCNLGTQQ
                     RFFDEHLRGWTVRMCEQIAAHPAADFYRAAALFAHAFFTVEAQAFDMLD"
     gene            complement(2233..4377)
                     /locus_tag="VFB53_07355"
     CDS             complement(2233..4377)
                     /locus_tag="VFB53_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZN86584.1"
                     /translation="MKTLICDCNRTMPLDPAALAKALPADAADGLQTLHSTLCRREAG
                     AFQRAAKEGDDLLVACTQESRLFLELNAETQGAPSVQERPIRFVNIRETGGWSKDAKA
                     AMPKIAALLAAAQLPPPEPVPTVSYRSAGRVLVIGDADAAQRAAAMLADACEVSLLLT
                     RPGGSLPQQRDIPVHEGRVQSITGWLGAFEVAWTSGNPIDLDLCTRCNACIEACPEGA
                     IDFSYQVDLARCTAHRDCVRVCEAAGAIDFTRAPQAASETFDAVLDLGAAALLGMHQP
                     PQGYFRPGADERRLFDAVVELRGLVGEFEKPKFVGYRPRLCAHSRNEQIGCDACIEVC
                     SARAIASEATTTGGIRVEPHLCVGCGACATVCPSGALTYATPSPDHLGRRLRTMLQTY
                     RRAGGPAATGPAALLVHSEGAGRRLLHELGRAARVDPAVHGVPARVLPVEVFHTASVG
                     LDLWMAAFAYGAADIWVLLTDEEAPGYREALQEQAAVANALMEGLGFAGGRVRLVQAR
                     DARDLAALDAALLAPHAPVEVAPAAFGVQADKRATLELALDHLLQSAPARAEAIALPA
                     AGSPFGSVTVDADRCTLCLACVGACPESALVDNPERPQLRFIEKNCVQCGLCATTCPE
                     RAVTLQPRLWLADEGRARKQARVLNEAEPFRCVRCGKPFGTLKAIEAMVGRLAGHAMF
                     QGAAAERLRMCGDCRVVDIHTSRDELHITDVR"
     gene            complement(4301..4954)
                     /locus_tag="VFB53_07360"
     CDS             complement(4301..4954)
                     /locus_tag="VFB53_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HZN86585.1"
                     /translation="MSGDDPFLARWSRRKAQAKQGGAAPEARPGTPPEALPPERTAGV
                     GAGEPGDRPAPASTLPQGGREDAPASTDAPEPPPPTLDDVATLPEGADISRFMAPKVD
                     DDVKRAALRKLFADPHFNTMDGLDTYIEDYGRPDPIPLAMLRTMAQSKVLGLFQDEAE
                     PAQETAAPQASPDGAAPPAVPQSATDCPAVPPDENPDLRLQQDHAARPRGTGEGAAG"
     gene            complement(4951..>5071)
                     /locus_tag="VFB53_07365"
     CDS             complement(4951..>5071)
                     /locus_tag="VFB53_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HZN86586.1"
                     /translation="VPLDPELAAWLQAYTDAHYRPEPKQRKRPASFQRPEDRK"
BASE COUNT          645 a         1803 c         1872 g          751 t
ORIGIN      
        1 ggaagatgtt ggcgccgccg cccgacacgc cgatgttgcc gagcgcgagc tgcacgatgc
       61 acgaggcgcg cacgatggcg ttgccgatgg tgtgctgcgt ctggcccatg caccacacga
      121 tcgtgctggg ccggttctcg gccatgatct tcgccgcatt gaggcaggtg gcctcgtcga
      181 cgccgcagac ctcctggacc ttgtcgggcg tccacttcgc gagcacctcg tcgcggatct
      241 tgtccatgcc gtacacgcgg tcggcgatgt acttcttgtc ttcccagccg ttcttgaaga
      301 tgtggtgcag catgccgaac aggaacggga tgtccgtgcc cgagcggatg cggatgtagt
      361 ggtcggcctt cgccgccgtg cgggtgaagc gcggatcgac cacgatcatc ttcgtgcccg
      421 tctccttcgc atgcagcatg tgcagcatcg acaccgggtg cgcctcggcg gcgttggagc
      481 cgatgtagag cgccgccttg gcgttctgca tgtcgttgta ggagttggtc atcgcgccgt
      541 agccccacgt gttcgcgacg cccgcgacgg tggtggagtg gcagatgcgc gcctggtggt
      601 cgcagttgtt gctgccccac aggctgaccc acttgcgcat caggtacgcc tgctcgttgc
      661 tgtgcttgga cgagccgacg aggaagacgg aatcggggcc gctctgcttc ctcagctcga
      721 gcatcttcgc gctgatctcg ttcagcgcct ggtcccagga gatgcgccgg tacttgccgt
      781 ccaccagctt catcgggtac ttcagccggt actcgccgtg gccgtgctcg cgcagcgcgg
      841 cgcccttggc gcagtgcgcg cccaggttga tgggcgagtc gaacaccggc tcctggcgga
      901 tccacacgcc gttctgcacc accgcgtcca ccgcgcagcc caccgagcag tggccgcaca
      961 cggtgcgctt cacctcgatc ctggggccgc cgctcttggg cgcgtcggcc gcgtgcgcct
     1021 tgcgcacgag ggtgagctgc gaggcggcca ggccggcgcc gacgcccagg cccgagcggc
     1081 gcaggaaggc gcgccggtcc atcgtgggaa tcgcgcgcga gacgccgcgc gcgaggctgg
     1141 acaccaggga agacgacggc gcggcgacac ccgcgccgga tgttttacgc gtgagcaaca
     1201 tgagcactcc tcgggggcgc cgagcgccca ccatcaaacc ttggtggtct cgtagtagcg
     1261 cagcacgtgc tgcgtgagct ggtatccacc cttggcggcg gcctccgggg ccgcggcgcc
     1321 cggcgcggcc gcgtcggcgg gggcggtggg ccaggcccgc gccgcaagca cggcggcgcc
     1381 ggcggcgccg gcgccgagca gcaggctgcg tcgctggttc ggggaggcgc cgggggcctc
     1441 cggaccggtt ggcttcacac tcgacttcat gaatgcactc ccaacaattt cccgactgtc
     1501 ccgtggccgc ccggtttttg tgcatcgacg ccacactgcg actgtatgca tcgtgttgca
     1561 tcgaacagcc ggggtttttc cgtagctgcg gtgcatcaat ccagcatgtc gaaggcctgc
     1621 gcctccaccg tgaagaacgc gtgtgcgaac agcgccgccg cgcgatagaa atcggccgcc
     1681 ggatgcgcgg cgatctgctc gcacatgcgc accgtccagc cgcgcaggtg ctcgtcgaag
     1741 aagcgctgct gcgtgccgag gttgcacacc gccggatcgt cgccggcgat gaggaagcgc
     1801 atcgcctcgc acaggtaggc gacgtggtct tccgtctcgt acatgccctc cacgcgttcg
     1861 aggccgaggc gcgccagcgt ctcgcgcagc gccaccagcg gcgtctcgtt gagcgcgccc
     1921 gcgaggtgat gcgagccgta caggaacacc tccggcttgc cgaggccgag gaagagcgcc
     1981 gcgtactcgc cggcgatctc ctcgaccgtg cgccggcgcg ccgccgccac cacctccgac
     2041 cagctgccct cgagcagtcc gccgggcgcg ggcgcgacgg tgggcgccac ccgcagctgc
     2101 gcatgcagct gcggcggcgg cggcgcgtag aacagcgcgg ccagcaggcc gtacgactcg
     2161 gcgcgcgcga gctcctcgcg gtcgtcgtcg ggcgcgggct cgaagcgaag cggccgggtg
     2221 gtcatcgcgg cgtcagcgca cgtccgtgat atgcagttcg tcgcggctgg tgtggatgtc
     2281 gacgacgcgg cagtccccgc acatcctgag ccgctcggcg gccgcgccct ggaacatggc
     2341 atggccggcg agccggccga ccatggcctc gatcgccttc agggtgccga acggcttgcc
     2401 gcagcgcacg cagcggaagg gctcggcctc gttcagcacg cgcgcctgct tgcgcgcgcg
     2461 cccctcgtcc gccagccaca ggcgcggctg cagcgtgacg gcgcgctcgg ggcaggtggt
     2521 ggcgcaaagc ccgcactgca cgcagttctt ctcgatgaag cgcagctgcg ggcgctcggg
     2581 gttgtcgacc agcgcgcttt ccggacaggc gccgacgcag gccaggcaca aggtgcaccg
     2641 gtccgcgtcc acggtcacgc tgccgaacgg gctgccggcg gccggcaggg cgatcgcctc
     2701 ggcgcgcgcc ggggcgcttt gcagcaggtg gtccagcgcg agctcgagcg tcgcgcgctt
     2761 gtcggcctgc acgccgaagg cggcgggcgc gacctcgacc ggcgcgtgcg gcgccagcag
     2821 ggcggcatcc agcgcggcca ggtcgcgcgc gtcgcgcgcc tgcaccagcc gcacgcggcc
     2881 gccggcgaag ccgaggcctt ccatcagcgc attggccacg gcggcctgct cctgcagcgc
     2941 ctcgcggtac ccgggcgctt cctcgtcggt gagcagcacc cagatgtcgg ccgcgccgta
     3001 ggcgaaggcg gccatccaca ggtcgaggcc cacgctcgcg gtgtgaaaga cttccaccgg
     3061 cagcacgcgc gccggcacgc cgtgcacggc gggatcgacg cgcgccgcgc ggcccagctc
     3121 gtgcagcagc cggcgcccgg cgccctcgct gtgcaccagc agcgcggcgg gcccggtggc
     3181 ggccggcccg ccggcgcggc gatacgtctg cagcatcgtg cgcagccgcc tgccgaggtg
     3241 gtcgggcgac ggcgtcgcgt aggtcagcgc gccgctcggg cagacggtgg cgcaggcgcc
     3301 gcagcccacg cacaggtgcg gctcgacccg gatgccgccg gtggtcgtgg cctcgctggc
     3361 gatggcgcgc gccgagcaca cctcgatgca ggcgtcgcag ccgatctgct cgttgcggct
     3421 gtgggcgcag agcctcggcc ggtacccgac gaacttcggc ttctcgaact cgccgacgag
     3481 gccgcgcagc tccaccaccg cgtcgaacag ccggcgctcg tccgcccccg ggcggaaata
     3541 gccctggggc ggctggtgca tgccgagcag cgcagcggcg cccaggtcga gcaccgcgtc
     3601 gaaggtttcg ctcgcggcct gcggcgcgcg ggtgaagtcg atggcgccgg ccgcctcgca
     3661 gacccggacg cagtcgcgat gcgccgtgca gcgggccagg tccacctggt agctgaagtc
     3721 gatcgccccc tcggggcagg cctcgatgca ggcgttgcag cgcgtgcaca ggtcgaggtc
     3781 gatcgggttg ccgctggtcc aggcgacctc gaacgcgccg agccagcccg tgatcgactg
     3841 cacccggccc tcgtgcacgg ggatgtcgcg ctgctgcggc aggctgccgc cggggcgcgt
     3901 gagcagcagg ctcacttcgc aggcgtcggc cagcatggcg gcggcacgct gcgcggcgtc
     3961 ggcgtcgccg atcacgagca cgcggccggc gctgcggtag gagaccgtgg gcaccggctc
     4021 gggcggcggc agctgggccg cggcgagcag cgccgcgatc ttcggcatcg cggccttggc
     4081 gtccttcgac cagccgccgg tctcgcggat gttcacgaag cggatcggcc gctcctgcac
     4141 gctcggcgcg ccctgcgtct cggcgttcag ctcgaggaac aggcggcttt cctgcgtgca
     4201 ggcgacgagc aggtcgtcgc cttccttggc cgcgcgctgg aacgcgcccg cctcgcggcg
     4261 gcagagggtg gaatgcagcg tctgcaggcc gtcggcggcg tcagccggca gcgccttcgc
     4321 cagtgccgcg gggtcgagcg gcatggtcct gttgcaatcg cagatcaggg ttttcatcgg
     4381 ggggtactgc cgggcaatcc gtggccgact gtggcaccgc cgggggcgcg gcgccatccg
     4441 gggatgcctg tggcgccgcg gtctcctgcg cgggctcggc ctcgtcctgg aacaggccga
     4501 gcaccttgga ctgggccatc gtgcgcagca tcgcgagcgg aatcgggtcc ggcctgccgt
     4561 agtcctcgat gtacgtgtcc aggccgtcca tcgtgttgaa gtgcgggtcg gcgaagagct
     4621 tgcgcagcgc ggcgcgcttg acgtcgtcgt cgaccttcgg ggccatgaag cggctgatgt
     4681 cggcgccctc cgggagggtg gcgacgtcgt cgagggtggg cggcggcggc tcgggagcat
     4741 ccgtgctcgc gggagcgtcc tccctccccc cctggggcag ggtcgaggcg ggggccggcc
     4801 ggtcgcccgg ctcgccggcc cccacccccg ccgtccgctc cgggggaagg gcctccggtg
     4861 gcgtgccggg acgggcttcg ggcgcagcgc ccccctgctt cgcctgcgcc ttcctgcgcg
     4921 accaccgcgc caggaacggg tcgtcgccgc tcacttgcga tcctccggcc gctggaacga
     4981 cgccgggcgc ttgcgctgct tgggctcggg ccggtagtgc gcgtcggtgt acgcctgcag
     5041 ccaggccgcg agctccgggt cgagcggcac g
//
LOCUS       DASRIE010000173         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1226996, whole genome shotgun sequence.
ACCESSION   DASRIE010000173 DASRIE010000000
VERSION     DASRIE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226996"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VFB53_07370"
     CDS             <1..1246
                     /locus_tag="VFB53_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZN86587.1"
                     /translation="QIEGSHAVAEAVALARPEVICAYPISPQTHIVEGLGEMVKSGAL
                     APCEFMNVESEFAAMSVAIGSSAAGARTYTATASQGLLFMAEAVYNASGLGLPIVMTV
                     ANRAIGAPINIWNDHSDSMSQRDCGWLQLFAETNQEALDLHIQAFKLGEELSMPVMVC
                     MDGFILTHAVERVDVPAQAEVDAFLPPYEPRQVLDPAAPVSIGAMVGPEAFMEVRYIA
                     HAKQTMALERIPQIADEFAERFGRASGGLVRPYRCEGAETIVVALGSVLGTLKDTVDE
                     LRAAGHRIGVLGIQSFRPFPLAAVRAALQEARRVVVLEKSFSVGLGGVVSTDVRMALS
                     GIQLHGYTVVAGLGGRAITRASLARTLREAMADTLAPLTFMDLDWAIVNRQLAREAQM
                     RRSGPIAENLLRDLGTVASKVS"
     gene            1251..2258
                     /locus_tag="VFB53_07375"
     CDS             1251..2258
                     /locus_tag="VFB53_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN86588.1"
                     /translation="MNAPIKFYQTGTFTVGNRLLSPQQRSVQASPERHNALTSGHRAC
                     QGCGEALGARYAVDAALRAAQGQLIAANATGCLEVFSTPYPETSWQLPWIHSLFGNAA
                     AVGSGIAAAMRVKGKSRVRVIAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNEAYM
                     NTGVQRSSATPPAARTATTMAVGEQPGADFGKGKNVPLIAMAHHIPYVATATVADLRD
                     LEAKVEKAMRLRGARYLHVLVPCPLGWGAASHDTIRLARLARETGIFPVFEAEHGEVS
                     AVTRIRRLLPVQDYLKPQKRFAHLFGNPGQPQMVEKIQAAANRNIERFGLLDKEEETL
                     A"
     gene            2255..3886
                     /locus_tag="VFB53_07380"
     CDS             2255..3886
                     /locus_tag="VFB53_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZN86589.1"
                     /translation="MTASKKPYAITLEVGSSLANKTGSWRTQRPVYVDRLPPCNAQCP
                     AGEDIQGWLFHAESGDYETAWRRLTRDNPFPAIMGRVCYHTCEGACNRGKLDQAVGID
                     SVERFLGDQALERGWRFEAPPRESGKSVLVVGAGPSGMSAAYHLRRLGHRVTVLDAGP
                     RMGGMMRFGIPKYRLPREVLDAEMQRIVDLGVEVKLDTRVDRIEEAMAGFDAAFLAVG
                     AHIAKRAFIPAKDSARILDAVSVLRSMDGEAPPLLGRRVVVYGGGNTAIDVARTARRL
                     GATEAVIVYRRTREKMPAHDFEVEEALEEGVLVKWLSTIRQAAEGALTIEKMVLDAHG
                     NPQPTGEFETLQADSLVLALGQDVDLSLLDGVPGLERKDGVVQVDAHTLMTGHPGLFA
                     GGDMVPAERNVTVAVGHGKKAARHIDAWLRGTRVAAAPKHAPASFERLNPWYYSDAPK
                     TLRPQLDVARRTSSFDEVQGGLTEDNALFEARRCLSCGNCFECDNCYGVCPDNAVVKL
                     GPGKRFQINLDYCKGCGVCMAECPCGAIEMAPEAV"
BASE COUNT          613 a         1464 c         1473 g          544 t
ORIGIN      
        1 gcagatcgaa ggctcgcatg ccgtggccga ggcggtggcg ctcgcgcgcc ccgaggtcat
       61 ctgcgcctac cccatctcgc cgcagacgca catcgtcgaa ggcctgggcg agatggtgaa
      121 gagcggcgcg ctcgcgccgt gcgagttcat gaacgtggag agcgagttcg ccgcgatgag
      181 cgtggcgatc ggcagctcgg ccgcgggcgc gcgcacctac accgcgaccg ccagccaggg
      241 cctgctgttc atggccgagg cggtctacaa cgcctccggc ctgggcctgc cgatcgtgat
      301 gacggtggcc aaccgcgcga tcggcgcgcc catcaacatc tggaacgacc acagcgactc
      361 gatgagccag cgcgactgcg gctggctgca gctgttcgcc gagaccaacc aggaggcgct
      421 ggacctgcat atccaggcct tcaagctcgg cgaggagctg tcgatgccgg tgatggtgtg
      481 catggacggc ttcatcctca cgcacgccgt cgagcgcgtc gacgtgccgg cgcaggccga
      541 ggtggacgcg ttcctgccgc cgtacgagcc gcgccaggtg ctcgacccgg cggcgccggt
      601 gtcgatcggc gcgatggtcg gccccgaggc gttcatggag gtgcgctaca tcgcgcacgc
      661 caagcagacg atggcgctcg agcgcatccc gcagatcgcc gacgagttcg ccgagcgctt
      721 cggccgcgcc tcgggcggcc tggtgcggcc ctatcgctgc gagggcgccg agacgatcgt
      781 cgtcgcgctg ggctcggtgc tcggcacgct gaaggacacg gtggacgagc tgcgcgcggc
      841 cggccacagg atcggcgtgc tcggcatcca gtcgttccgc ccgttcccgc tggccgccgt
      901 gcgcgcggcc ttgcaggagg cgcgccgcgt ggtcgtgctc gagaagagct tctcggtggg
      961 cctgggcggc gtggtgtcca ccgacgtgcg catggcgctg tcgggcatcc agctgcacgg
     1021 ctacaccgtg gtcgccgggc tcggcgggcg cgccatcacc cgcgcgtcgc tcgcgcgcac
     1081 cctgcgcgag gcgatggccg acacgctggc gccgctcacc ttcatggacc tggactgggc
     1141 gatcgtcaac cggcaactgg cacgcgaggc gcagatgcgc cgcagcggcc cgatcgccga
     1201 gaacctgctg cgcgacctgg gcacggtcgc ctccaaagtg agctgacgcg atgaacgcac
     1261 cgatcaagtt ctaccagacc ggcaccttca ccgtcggcaa ccgcctgctc tcgccgcagc
     1321 agcgcagcgt gcaggcgagc cccgagcgcc acaacgcgct cacgtcgggc caccgcgcct
     1381 gccagggctg cggcgaggcg ctcggcgcgc gctacgcggt ggacgcggcg ctgcgcgccg
     1441 cgcagggcca gctgatcgcg gccaacgcga ccggctgcct cgaggtgttc tccacgccct
     1501 accccgagac gtcgtggcag ctgccgtgga tccactcgct gttcggcaac gccgcggcgg
     1561 tgggcagcgg catcgccgcg gcgatgcgcg tcaagggcaa gtcgcgcgtg cgcgtgatcg
     1621 cccaaggcgg cgacggcggc accaccgaca tcggcttcgg ctgcctgtcg ggcatgttcg
     1681 agcgcaacga cgacgtgctc tacatctgct acgacaacga ggcctacatg aacaccggcg
     1741 tgcagcgcag ctcggcgacg ccgccggccg cgcgcaccgc caccaccatg gcggtgggcg
     1801 agcagccggg cgcggacttc ggcaagggca agaacgtgcc gctgatcgcg atggcgcacc
     1861 acatccccta tgtcgccacc gcgacggtgg cggacctgcg cgacctggaa gccaaggtcg
     1921 agaaggcgat gcgcctgcgc ggcgcgcgct acctgcatgt gctcgtgccc tgcccgctcg
     1981 gctggggcgc ggccagccac gacaccatcc gcctcgcgcg gctcgcgcgc gagaccggca
     2041 tcttcccggt gttcgaggcc gagcacggcg aggtgagcgc cgtcacgcgg atccgccgcc
     2101 tgctgccggt gcaggactac ctgaagccgc agaagcgctt cgcccacctg ttcggcaacc
     2161 ccggacaacc gcagatggtc gagaagatcc aggccgccgc caaccgcaac atcgagcgct
     2221 tcgggctgct cgacaaggaa gaggagacgc tcgcatgacc gcgtcgaaga agccctacgc
     2281 gatcacgctg gaggtcggct cgtcgctggc caacaagacc ggcagctggc gcacgcagcg
     2341 ccccgtgtac gtcgaccggc tgccgccgtg caacgcgcag tgccccgcgg gcgaggacat
     2401 ccagggctgg ctgttccacg ccgagagcgg cgactacgag acggcgtggc gccgtctcac
     2461 gcgcgacaac ccgttccccg cgatcatggg gcgcgtgtgc taccacacgt gcgaaggcgc
     2521 ctgcaaccgc ggcaagctcg accaggcggt gggcatcgac tcggtggagc gcttcctggg
     2581 cgaccaggcg ctcgagcgcg gctggcgctt cgaggcgccg ccgcgcgaga gcggcaagtc
     2641 ggtgctggtc gtgggcgcgg ggccctcggg catgtccgcg gcctatcacc tgcgccgcct
     2701 cggccaccgc gtcaccgtgc tcgacgccgg cccgcgcatg ggcggcatga tgcgcttcgg
     2761 catccccaag taccgcctgc cgcgcgaggt gctcgacgcc gagatgcagc gcatcgtcga
     2821 cctgggcgtc gaggtgaagc tcgacacgcg cgtcgaccgc atcgaggagg cgatggccgg
     2881 cttcgacgcg gccttcctcg cggtcggtgc gcacatcgcc aagcgcgcct tcatcccggc
     2941 caaggacagc gcgcgcatcc tcgacgcggt gtcggtgctg cgctcgatgg acggcgaggc
     3001 gccgccgctg ctcggccggc gcgtcgtcgt gtacggcggc ggcaacacgg ccatcgacgt
     3061 ggcacgcacc gccaggcgcc tcggcgcgac cgaggcggtg atcgtctatc ggcgcacgcg
     3121 cgagaagatg cccgcgcacg acttcgaggt cgaggaggcg ctcgaggaag gcgtgctggt
     3181 gaagtggctg tccaccatcc ggcaggccgc cgagggcgcg ctgacgatcg agaagatggt
     3241 gctcgatgcg cacggcaacc cgcagcccac gggcgagttc gagaccttgc aggccgattc
     3301 gctggtgctc gcgctggggc aggacgtgga cctctcgctg ctcgacggcg tgcccggact
     3361 cgagcgcaag gacggcgtgg tccaggtcga tgcgcacacg ctgatgaccg gccacccggg
     3421 gctgttcgcc ggcggcgaca tggtgccggc cgagcgcaac gtgaccgtcg ccgtgggcca
     3481 cggcaagaag gccgcgcgcc acatcgacgc gtggctgcgc ggcacgcgcg tggccgcggc
     3541 gcccaagcac gcgccggcga gcttcgagcg gctgaacccc tggtactaca gcgacgcgcc
     3601 caagaccctg cgcccgcagc tcgacgtggc gcgccgcacc agcagcttcg acgaggtgca
     3661 aggcggcctc accgaggaca acgcgctgtt cgaggcgcgc cgctgcctga gctgcggcaa
     3721 ctgcttcgag tgcgacaact gctacggcgt gtgccccgac aacgcggtgg tcaagctcgg
     3781 tcccggcaag cgcttccaga tcaatctcga ctactgcaag ggctgcggcg tgtgcatggc
     3841 cgaatgcccg tgcggcgcca tcgagatggc gcccgaggcc gtctgaccga gggcgtccct
     3901 gcgcgcgggg cgcctgcacg gccccgagcg gcaaggcaag ccatggcgcg ggcatccccc
     3961 gcgccatttc gtttctgtaa cgcgcgggag gcgggtgcgc ggaacgcgct ggccgcaggc
     4021 gctgtgcgac agtggcgcga tgcccgccac cgccctgctc gtgtgcgtcg tcatcggcat
     4081 cgccgacggc gaca
//
LOCUS       DASRIE010000174         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1993705, whole genome shotgun sequence.
ACCESSION   DASRIE010000174 DASRIE010000000
VERSION     DASRIE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993705"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..446
                     /locus_tag="VFB53_07385"
     CDS             78..446
                     /locus_tag="VFB53_07385"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HZN86590.1"
                     /translation="MERHEYVVQGRLDLVRGNMVRIEDGRGMLVRVTSGSVWITEEGD
                     ARDRFVSAGGRFRVVSSGLTLVSAISRTTIALSSPYEHGFARRIDLVRASWLMRLRAR
                     LLKAWKGWYVPGARPTTAAL"
     gene            443..1396
                     /locus_tag="VFB53_07390"
     CDS             443..1396
                     /locus_tag="VFB53_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZN86591.1"
                     /translation="MTAQYRTGLPQLGDALFVTDGGIETALIFNEGIELPDFAAFDLF
                     RRPGGEAALRRYYGAYVRIAAQFRAGMILESATWRASADWGRKLGYSAAQLAEANRQA
                     IRLLEDIRREHRELSMVISGCVGPRGDGYSPSRVMSTDEAQGYHREQIAIFSDTAADM
                     VAAITMNYVEEAIGVARAAQDAGMPVAISFTVETDGRLPTGQPLQEAIEQVDGATALY
                     PVYYMLNCAHPEHFQKVLEKPWARRIRGLRANASRKSHAELNESATLDIGDPAELGAD
                     YAALKRGLLPNLNVVGGCCGTDHRHVQRIAAACAPLFRVAA"
     gene            1408..1887
                     /locus_tag="VFB53_07395"
     CDS             1408..1887
                     /locus_tag="VFB53_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HZN86592.1"
                     /translation="MKRISIVLSVMTAACASTVQVPDNLKPDAGESLARIVPAKGVQI
                     YECRDAKWAFVAPEAELFDRAGKKIGTHYAGPHWEAADGSKVVGAVKARADAPGEGNI
                     PWLLLGAKSVGNDGAFSKVTSIQRVATAGGVAPAGACSSGKRVSVPYTADYYFYTAR"
BASE COUNT          326 a          686 c          707 g          281 t
ORIGIN      
        1 tggagctcga ggccaacagc gcgcgcgcgc gcggccggcg cctcgacctg cgcttcagca
       61 tgtactgaga gcgcgccatg gaacgacacg aatacgtagt gcaaggccgc ctcgacctgg
      121 tgcgcggcaa catggtgcgc atcgaggacg gccgcggcat gctggtgcgc gtcaccagcg
      181 gcagcgtgtg gatcacggaa gagggcgacg cgcgcgaccg cttcgtcagc gccggcggca
      241 ggttccgcgt ggtctccagc ggcctcacgc tggtgtcggc gatcagccgc accacgatcg
      301 cgctcagctc gccgtacgag cacggcttcg cccgccgcat cgacctggtg cgcgcgagct
      361 ggctcatgcg cctgcgcgcg cggctgctca aggcgtggaa gggctggtac gtcccgggcg
      421 cgcggccgac cacggcggcg ctatgaccgc ccagtaccgc accggcctgc cgcagctcgg
      481 cgacgcgctg ttcgtcaccg acggcggcat cgagaccgcg ctcatcttca acgagggcat
      541 cgagctgccg gatttcgcgg cgttcgacct gttccggcgc ccgggcgggg aggccgcctt
      601 gcgcaggtat tacggcgcct acgtgcgcat cgccgcgcaa ttccgcgccg gcatgatcct
      661 ggagagcgcc acctggcgcg cgagcgcgga ctggggccgc aagctcggct acagcgcggc
      721 gcagctcgcc gaagcgaacc gccaggcgat ccgcctgctg gaggacatcc gccgcgagca
      781 ccgcgagctg tcgatggtga tcagcggctg cgtcggcccg cgcggcgacg gctacagccc
      841 gagccgcgtc atgtcgaccg acgaggcgca gggctatcac cgcgagcaga tcgcgatctt
      901 cagcgacacc gcggccgaca tggtggcggc gatcaccatg aactacgtcg aggaggcgat
      961 cggcgtggcg cgcgcagcgc aggacgccgg catgccggtg gcgatctcct tcaccgtcga
     1021 gaccgacggc cgcctgccga ccgggcagcc gctgcaggaa gcgatcgagc aggtggacgg
     1081 cgcgaccgcg ttgtacccgg tctactacat gctgaattgc gctcacccgg agcatttcca
     1141 gaaggtgctg gagaagccct gggcacggcg catccgcgga ttgcgcgcga atgcctcgcg
     1201 caagagccac gccgagctga acgaatcggc gacgctggat atcggcgatc ccgccgagct
     1261 gggcgcggac tacgccgcgc tcaagcgcgg cctgcttccg aatctcaacg tggtcggcgg
     1321 ctgctgcggc accgaccacc gccacgtgca gcgcatcgct gccgcctgcg cgccgctttt
     1381 ccgggtcgca gcttaaaaag ggaaaccatg aaacgcatca gcatcgtgtt gtccgtcatg
     1441 accgccgcgt gtgcgagcac tgtccaggtg cccgataacc tcaagccgga cgcgggcgaa
     1501 tcgcttgcgc gcatcgtgcc ggcgaagggg gtacagatct acgagtgccg cgacgccaag
     1561 tgggcgttcg tcgcgccgga ggcggagctc ttcgaccgcg ccgggaagaa gatcggcacg
     1621 cactacgccg gtccgcactg ggaggcggcc gacggcagca aggtcgtcgg cgccgtgaaa
     1681 gcccgcgccg atgcgccggg cgaggggaac atcccgtggc tgctgctcgg cgcgaaatcg
     1741 gtcggaaacg acggcgcctt cagcaaggtg accagcattc agcgtgtcgc caccgcgggc
     1801 ggtgtggccc ccgcgggcgc ctgtagttcg ggcaagcgcg tcagcgtgcc gtacacggcg
     1861 gattactact tctacacggc caggtaggga cggcagtcct ggccgatccc ggcgtcttcg
     1921 ccggattccg ggaccgccgc ctcaggcgcc gtgccgagga agcaaggaca ctaagccatg
     1981 actttcccgt ggttcatggt
//
LOCUS       DASRIE010000175         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_411685, whole genome shotgun sequence.
ACCESSION   DASRIE010000175 DASRIE010000000
VERSION     DASRIE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411685"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFB53_07400"
     CDS             <1..258
                     /locus_tag="VFB53_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZN86593.1"
                     /translation="SPLRRLAALAALPVLKALRRRIDPGRYNGASLLGLRGVVIKSHG
                     SADVAAFGQALERAVEQVRNDIIQRISQRMARAHGTAQAAE"
     gene            255..1214
                     /locus_tag="VFB53_07405"
     CDS             255..1214
                     /locus_tag="VFB53_07405"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZN86594.1"
                     /translation="MTFARIVGTGSYLPPRIVTNAEFAARLDTSDAWIRERTGITQRH
                     IAEESQASSDLALQASQAALAAAGVRAADLDLIIVATSTPDYIFPSTACLLQAKLRVA
                     NAAAFDLQAVCSGFVYALATADSFIRAGTHRRALVVGAEVFSRILDWNDRGTCVLFGD
                     GAGAVVLAAGERPGVHASVLRADGSHAGILSVPGNVCGGRIVGSPFLQMQGGQVFKFA
                     VKVLDEVARETVAAAGMRLEDIDWLIPHQANVRILEATAKKLGLPLDKLVVTVDHHGN
                     TSAASVPLALDEYVRAGRIRAGHRVMMQGVGGGFTWGATLATM"
     gene            1218..2156
                     /gene="fabD"
                     /locus_tag="VFB53_07410"
     CDS             1218..2156
                     /gene="fabD"
                     /locus_tag="VFB53_07410"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZN86595.1"
                     /translation="MKLAMVFPGQGSQAVGMLQAYAGLPALDELRRAIGGALGEEFLR
                     LLDEGPAEALGLTVNTQPAMVSAGYAAYRAWRALGGPEPEVVAGHSLGEYTALVAAGA
                     LEFRDCLPLVRYRAQAMQEAVPEGMGAMAAILGLEDDAVRAACAEAAQGEVVQAVNFN
                     APAQVVIAGHRDAVARGMEACKARGAKRALPLPVSAPFHSSLMRPAAERLRSYLADVK
                     LSVPKLQLINNVDVGIETQPAAIKDALVRQAAAPVRWVETIRRMRQLGVTHVLECGPG
                     KVLAGMVKRIDAGLQSHALADKASLEQALAALQAAA"
     gene            2153..2869
                     /gene="fabG"
                     /locus_tag="VFB53_07415"
     CDS             2153..2869
                     /gene="fabG"
                     /locus_tag="VFB53_07415"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN86596.1"
                     /translation="MTLQGKLALVTGASRGIGQAIAAELARQGATVIGTATTEAGAGK
                     IPGTGRVLDVRDAAACDALIDSLQKDFGDILILVNNAGITRDNLALRMKDAEWDEVID
                     INLKAVFRLSRAVMRGMMKARWGRIINITSVVGAAGNAGQSNYAAAKAGVVGMTKSLA
                     REFGGRGITVNSVAPGFIDTDMTRALTEAQRTALLGQIPLGRLGAPEDVAAAVAYLAS
                     PAAAYVTGCVLHVNGGMYMA"
BASE COUNT          423 a          997 c         1106 g          395 t
ORIGIN      
        1 agccccctgc gccggctggc ggcgctggcg gcgctgccgg tgctcaaggc gctgcggcgc
       61 cgtatcgacc cggggcgcta caacggcgcc agcctgctcg gcctgcgcgg cgtggtgatc
      121 aagagccacg gctcggccga cgtggctgcc ttcggccagg cgctggagcg cgcggtagag
      181 caggtgcgca acgacatcat ccagcgcatt tcgcagcgca tggcgcgcgc gcacggcacc
      241 gcgcaggcgg ccgaatgaca tttgcgcgca tcgtcggcac cggcagctac ctgccgccgc
      301 gcatcgtgac caatgcggag ttcgcggcgc gcctggacac cagcgacgcg tggatccgcg
      361 agcgcaccgg catcactcag cggcacatcg cagaggagtc gcaggcctcg agcgacctcg
      421 cgctgcaggc gagccaggcg gcgctcgcgg cggccggcgt ccgggcggcg gacctggatc
      481 tgatcatcgt cgccacctcg acgccggact acattttccc cagcaccgcc tgcctgctgc
      541 aggccaagct tcgcgtggcc aatgccgcgg ccttcgacct gcaggcggtc tgcagcggct
      601 tcgtttacgc gctggccacc gccgacagct tcatccgcgc cggcacgcac cggcgcgcgc
      661 tggtggtggg cgccgaagtg ttttcgcgca tcctcgactg gaacgaccgc ggcacctgcg
      721 tgctgttcgg cgacggcgcc ggcgccgtgg tgctggcagc cggcgagcgg cccggcgtgc
      781 acgcctcggt gctgcgcgcc gacggcagcc acgccggcat cctgtcggtc ccgggcaacg
      841 tctgcggcgg ccgcatcgtc ggctcgccgt tcctgcagat gcagggcggc caggtgttca
      901 agttcgcggt caaggtgctc gacgaggtgg cgcgcgagac ggtggccgcg gccggcatgc
      961 ggctggagga catcgactgg ctgatcccgc accaggcgaa cgtgcgcatc ctggaggcga
     1021 cggcgaagaa gctcgggctg ccgctcgaca agctggtggt gaccgtggat caccacggca
     1081 acacgtcggc ggcctccgtg ccgctggcgc tcgacgaata cgtgcgcgcg ggcaggatcc
     1141 gcgccgggca tcgcgtcatg atgcaaggcg tcggcggcgg cttcacctgg ggcgcgactc
     1201 tcgcgacgat gtagcccatg aaactggcga tggtatttcc ggggcagggc tcgcaggcgg
     1261 tcggcatgct gcaggcgtac gccggcctgc ccgcgctcga cgagctgcgg cgcgcgatcg
     1321 gcggcgcgct cggcgaggaa ttcctgcgcc tgctggacga gggcccggcg gaggcgctcg
     1381 gcctgacggt gaatacgcag cccgccatgg tcagcgccgg ctacgccgcc taccgcgcgt
     1441 ggcgcgcgct gggcgggccg gagccggaag tggtggcggg gcacagcctc ggggaataca
     1501 ccgcgctggt ggccgccggc gcgctcgaat tccgcgattg cctgccgctg gtgcgctacc
     1561 gcgcgcaagc catgcaggag gcggtgccgg aggggatggg cgcgatggcc gcgatcctcg
     1621 gcctggagga cgacgcggtg cgcgcggcct gcgccgaggc cgcgcagggc gaggtggtgc
     1681 aggcggtgaa tttcaacgcg ccggcgcagg tggtgatcgc cggccaccgc gacgcggtgg
     1741 cgcgcggcat ggaggcctgc aaggcgcgcg gcgccaagcg cgcgctgccg ctgccggtga
     1801 gcgcgccgtt ccattccagc ctgatgcgcc ccgccgcgga gcgcctgcgc agctatctcg
     1861 ccgacgtgaa gctgtcggtc cccaagctgc aactgatcaa caacgtggac gtcggcatcg
     1921 agacgcagcc cgccgccatc aaggacgcgc tggtgcggca ggcggcggcg ccggtgcgct
     1981 gggtcgagac catccgccgg atgcggcagc tgggcgtgac gcatgtcctg gagtgcggcc
     2041 ccggcaaggt gctggccgga atggtgaagc gcatcgacgc cgggctgcag agccatgcgc
     2101 tcgccgacaa ggcgtcgctc gagcaggcgc tcgccgcgct gcaggcggct gcatgacgct
     2161 gcagggcaag ctcgccctgg tcacgggcgc cagccggggc atcggacagg cgatcgccgc
     2221 cgagctggcc aggcagggcg cgacggtgat cggcacggcg accacggagg caggcgcagg
     2281 gaagatcccc gggaccggcc gcgtgctcga cgtccgggat gccgcggcat gcgatgcgct
     2341 gatcgattcg ctgcagaagg atttcggcga catcctgatc ctggtgaaca acgccggcat
     2401 cacgcgcgac aacctggcgc tgcgcatgaa ggacgcggaa tgggacgagg tgatcgacat
     2461 caacctgaaa gccgtgttcc ggctgtcgcg cgcggtgatg cgcggcatga tgaaggcgcg
     2521 ctggggccgc atcatcaaca tcacgtcggt ggtcggggcg gccggcaacg ccggccagtc
     2581 caactacgcc gccgccaagg cgggggtggt cggcatgacc aagtcgctgg cgcgcgaatt
     2641 tggcggccgc ggcatcaccg tcaacagcgt cgcgccgggg ttcatcgata ccgacatgac
     2701 gcgcgcgctg accgaggcgc agcgcactgc gctcctcggc cagattccgc tgggacggct
     2761 gggagcgccg gaggatgtcg ccgccgcagt ggcctacttg gcgtcgccgg cggcggccta
     2821 tgtcaccgga tgcgtgctac acgtgaacgg cggcatgtac atggcctgag gccgcgcgcg
     2881 ccgatttggt aaaatccggg ccgccgcttg gccccaacag g
//
LOCUS       DASRIE010000176         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3570620, whole genome shotgun sequence.
ACCESSION   DASRIE010000176 DASRIE010000000
VERSION     DASRIE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570620"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VFB53_07420"
     CDS             complement(<1..1438)
                     /locus_tag="VFB53_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86597.1"
                     /translation="MAAVALAASPQARKLRVGLFADAPLQPRWVVDAFAKAAADGCAE
                     IVLIACADAAPRAVPWWWKLYGALDRKAFGARTDPGAGLELARHVPHQRSGALGADPR
                     ELLGLDLDVAFALGEIDDRVLDGVARLGVWRFQVDGMAEVAAGEPLSGAGLKVRLAAG
                     DTPRLAYQSWSRTFAFSVARNRDQLFAKTAEFATRALREAQRSGRGWLEQCPPVAERT
                     EAGGTPPAQPCNPSPILGRIARRALQNALHVEQWFLAFRFAGGDARAGQAELRADLQG
                     FTRLMPPKDRFWADPFALEKNGRYFIFFEDLPFAAGKAHIAVTEVRRDGTWSPPVPVL
                     ERDYHLSYPFLLEHEGQLYMIPETAQNGTVEIYRCVDFPLRWKLEKTLLQGVRLVDAT
                     LHRDAERWWMFANSSTGRVFNDELHLFHAERLLGEWRAHPRNPVKSDARCARPAGKLY
                     WQNGALHRPAQICVPRYGAGLSLNRVLQL"
     gene            complement(1426..>2032)
                     /locus_tag="VFB53_07425"
     CDS             complement(1426..>2032)
                     /locus_tag="VFB53_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZN86598.1"
                     /translation="KLGLDTVEVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAE
                     LLGYHPQVILAGRRINDGMGPHVARKAIQQMIHAGRNIKGARVNVLGLTFKENCPDIR
                     NSKVADILAELREFGVEAHVHDPMASAEDALHEYGVKLRSWDELPAADALILAVPHKR
                     YLEMPTAQIVKKIVRGGCVIDVKSVLDAQALRREGLRVWRL"
BASE COUNT          283 a          731 c          735 g          283 t
ORIGIN      
        1 tgagttgcag cacgcggttg agcgacagcc cggcgccgta gcgcggcacg cagatctgcg
       61 ccggcctgtg cagcgcgccg ttttgccagt acagcttgcc cgcgggccgg gcgcagcgcg
      121 cgtcggactt gaccgggttg cgcgggtgcg cgcgccactc gccgagcagg cgctcggcat
      181 ggaaaaggtg cagctcgtcg ttgaacacgc gccccgtgct gctgttcgcg aacatccacc
      241 agcgctcggc gtcgcgatgc agggtcgcgt ccaccagccg cacgccctgc agcagcgtct
      301 tctccagctt ccagcgcagc ggaaagtcga cgcagcggta gatctcgacc gtaccgttct
      361 gggcggtctc cggaatcatg tagagctgcc cctcgtgctc gagcagaaac ggatacgaga
      421 ggtggtagtc gcgctcgagc accggcaccg gcggcgacca ggtgccgtcg cggcgcacct
      481 cggtcacggc gatatgcgcc ttgcccgcgg cgaacggcag gtcctcgaag aagatgaaat
      541 agcggccgtt cttctcgagc gcgaacgggt cggcccagaa gcggtccttc ggcggcatca
      601 ggcgggtaaa gccctgcagg tcggcccgca gctcggcctg ccccgcgcgc gcgtcgccgc
      661 ccgcgaagcg gaaggcgaga aaccattgct cgacgtgcag cgcgttctgc agcgcgcggc
      721 gcgcgatgcg gccgaggatc gggctcgggt tgcagggctg cgcgggcggc gtgccgcccg
      781 cctcggtccg ctcggccacc ggcgggcact gctcgagcca gccgcggccg gagcgctgcg
      841 cctcgcgcag ggcgcgggtc gcgaactccg cggtcttcgc gaacagctgg tcgcggttgc
      901 gcgccaccga gaacgcaaag gtgcgcgacc acgactggta ggcgaggcgc ggcgtgtcgc
      961 ccgcggccag gcgcaccttg aggccggcgc cgctgagcgg ctcgccggcc gccacttcgg
     1021 ccatgccgtc gacctggaag cgccagaccc cgagccgcgc cacgccgtcg agcacgcggt
     1081 cgtcgatctc gccgagcgcg aacgccacgt cgagatccag gccgagcagc tcgcgcgggt
     1141 ccgcgccgag cgcgccgctg cgctggtgcg gcacgtggcg cgcgagctcg aggcccgcgc
     1201 cgggatcggt gcgcgcgccg aacgccttgc ggtcgagcgc gccgtacagc ttccaccacc
     1261 agggcacggc gcgcggcgcc gcgtcggcgc aggcgatgag cacgatctcg gcgcagccgt
     1321 ccgccgccgc cttggcgaag gcgtcgacca cccagcgcgg ctgcagcggc gcatcggcga
     1381 acagcccgac gcgcagcttg cgcgcctgcg ggctggccgc gagcgctaca gccgccatac
     1441 ccgcaggcct tcacgccgca acgcctgcgc gtcgagcacc gacttcacgt cgatcacgca
     1501 gccgccgcgc acgatcttct tgacgatctg cgccgtcggc atctcgaggt agcgcttgtg
     1561 cggcacggcg aggatcagcg cgtcggccgc cggcagctcg tcccacgagc gcagcttgac
     1621 gccgtactcg tgcagcgcgt cctcggcgct cgccatcggg tcgtgcacgt gcgcctcgac
     1681 gccgaactcg cgcagctcgg ccaggatgtc ggccaccttg gagttgcgga tgtcggggca
     1741 gttctccttg aaggtcaggc cgagcacgtt gacgcgcgcg cccttgatgt tgcgcccggc
     1801 gtggatcatc tgctggatcg ccttgcgcgc gacgtgcggc cccatgccgt cgttgatgcg
     1861 ccgcccggcg aggatcacct gcgggtggta gccgagcagc tccgccttgt gcgtgaggta
     1921 gtacgggtcg acgccgatgc agtggccgcc gaccaggccg ggacggaacg gcaggaagtt
     1981 ccacttggtg ccggcggcct tgagcacctc cacggtgtcg aggccgagct tc
//
LOCUS       DASRIE010000177         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_182305, whole genome shotgun sequence.
ACCESSION   DASRIE010000177 DASRIE010000000
VERSION     DASRIE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182305"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1659)
                     /locus_tag="VFB53_07430"
     CDS             complement(34..1659)
                     /locus_tag="VFB53_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86599.1"
                     /translation="MRRTPVKTRGLVLLGGLAGLLCIAFILLRTGFPQLPERQYIFDY
                     LLRAQDIPGGAVVIFIAIAAAWPPLARPGLALVAAMGGKPWATALITFLVLCAGQLLV
                     AKDHALAGDEHLILLQAKAFAAGRLTAQFPPELVTWVVPQPYINLWLYASPQTGEVVS
                     VYWPGFALLLAPFALLGIPWACNALLASGALLLIGRLAGRLAGAPQAAGWAMLLTLAS
                     PAFTGMALSYFSMTAHLFFNLLYVWLLLERRLFVAGVAGSFALVLHNPWPHSLFALPW
                     IGWIAWQDGRRAVLRLIAGYLPLTLAIGAGWWLLMRKVQGPVWFAPQGGEFLFHWQLQ
                     LTRVFTFPDAATTTHRAAELVKLWLWTVPGLPALMVAGWWLARREVPLRVLGLSFAIT
                     LAGYLMVWFDQGYGWGVRYLHPALAALPVLGAAALVSLPSEPLRRYAAGAALLSLAFA
                     VPFRWGQIESFMREQLARRPPFEKQVRQIVFVTPDYPYYTQDFVQNDPFLRAPVIFML
                     SRGRAADEKLLRERFPGARLAHETARGHVWRVD"
     gene            complement(1641..>2596)
                     /locus_tag="VFB53_07435"
     CDS             complement(1641..>2596)
                     /locus_tag="VFB53_07435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN86600.1"
                     /translation="RAAGVPYPFWSPAALLRLARSVRSANVVHLHDCLYLPVLAAFAA
                     ARFGRRRPVVVTQHIGHVPYRNPLLRAVHAAANRLLGAWVLGGADQVVFESESVRAYF
                     AQRVRFRSPPLLIENGVDTQVFRPCSAAERRERREQLGIPAGKPLLLFIGRFVEKKGM
                     NVLRELAGNIRGAHWLFAGWGPMDPAAWALANVTVRLRPGHEELPALYQAADLLVLPS
                     VGEGFPLSVQEAMACGTPALIGEDTAAGCPQGADALLSERVGGTDTAERWAARIQALL
                     AAPGELEALRPRVAAFARTHWSWERCGERYAALLQACAARQ"
BASE COUNT          487 a          887 c          920 g          302 t
ORIGIN      
        1 cggcggccga tgggctttac gcgcccggcg cgctcaatcc acgcgccaga cgtgaccgcg
       61 cgcggtctcg tgggcgagcc gcgcgcccgg aaagcgctcg cgcaagagct tctcatcggc
      121 ggcgcggccg cggctcagca tgaagatcac cggcgcgcgc aggaacggat cgttctgcac
      181 gaaatcctga gtgtagtagg ggtagtccgg cgtcacgaac acgatctggc gcacctgttt
      241 ctcgaatggc gggcggcgcg cgagctgctc gcgcatgaag gactcgatct gtccccagcg
      301 aaagggcacg gcgaacgcga ggctgagcag cgccgccccg gcggcgtagc gccgcaaagg
      361 ttcgctaggc aggctcacga gggccgctgc gccgagaacc ggcagggcgg caagcgcggg
      421 gtgcaggtag cgcacgcccc agccgtagcc ttggtcgaac cagaccatga ggtagcccgc
      481 gagggtgatg gcgaacgaga ggccgagcac ccgcaacggc acttcgcgcc gcgcgagcca
      541 ccagcccgcg accatcagcg cgggtagccc cggaacggtc catagccaca gcttgacgag
      601 ctcggcggcc cggtgcgtgg tggtcgctgc atccggaaag gtgaacacgc gcgtgagctg
      661 gagctgccag tggaaaagaa actctccgcc ttgcggcgca aaccagaccg gcccttgcac
      721 cttgcgcatc agcagccacc agccggcgcc gatagcgagc gtcaggggga ggtaaccggc
      781 gatcagccgc aggactgcgc gccgcccgtc ctgccaagcg atccagccga tccagggcag
      841 ggcaaagagc gagtgcggcc agggattgtg cagcacgagg gcgaaggagc cggcgactcc
      901 cgcaacgaaa aggcggcgct cgaggagcag ccagacgtaa agcaggttga aaaacaggtg
      961 cgcggtcatc gagaaatagc tgagcgccat gccggtgaac gcgggcgagg cgagcgtgag
     1021 cagcatcgcc cagcctgcgg cctgcggcgc gccggcgagg cgcccggcga gacggccgat
     1081 gagcaggagc gcgccgctcg ccagaagggc attgcaggcc cacggaatgc ccagcagggc
     1141 gaacggcgcg agcagcagcg caaaccccgg ccagtacacc gacaccactt cgccggtctg
     1201 cggcgaggcg tagagccaca ggttgatgta aggctgcggc accacccagg tgacgagctc
     1261 cggcggaaac tgggcggtca ggcggccagc ggcgaacgcc ttggcctgca gaaggatgag
     1321 gtgctcatcg ccggcgagcg cgtgatcctt cgcgactagc agctggccgg cgcacaggac
     1381 gagaaaggtg atgagcgccg tagcccacgg cttgcccccc atcgccgcga ccagcgcgag
     1441 tccgggccgc gcgagcggtg gccacgcggc ggcaatggcg atgaagatca cgactgcacc
     1501 gccgggtata tcctgcgcgc gcagcagata gtcgaagatg tactggcgct ccggcaactg
     1561 cggaaacccg gtgcggagca ggatgaaagc gatacacagc agcccggcga gcccgccgag
     1621 cagcacgagc ccgcgcgtct tcactggcgt gcggcgcacg cttgcaggag cgccgcgtag
     1681 cgctcaccgc accgctccca cgaccagtgc gtgcgtgcga aggcggcgac gcgcggccgc
     1741 agcgcctcca gctcgcctgg cgcggcgagc agtgcctgga tccgcgccgc ccatcgctct
     1801 gccgtatcgg tgccgccgac gcgctcgctg agcagcgcgt cggcgccttg cgggcagccc
     1861 gcggcggtgt cctcgccgat caaagccggc gtgccacagg ccatggcctc ctgcaccgag
     1921 agcgggaacc cctcgccgac gcttggcagc acgagcaggt ctgctgcctg gtagagcgca
     1981 ggcagctcct cgtggccggg tcgcagccgc accgttacgt tcgcaagcgc ccatgccgcc
     2041 gggtccatcg gaccccagcc agcgaacagc caatgggcgc cccggatgtt cccggcaagt
     2101 tcgcgcagaa cgttcatccc tttcttttcc acgaaccggc cgatgaacag gagcagcggc
     2161 ttacccgcag ggattccaag ttgctcgcgg cgctcgcgtc gctcggccgc ggagcagggc
     2221 cggaagacct gcgtatccac gccattttcg atcagcagcg gcggcgagcg aaaccgcacc
     2281 cgttgcgcga aataggcgcg caccgactcg ctttcgaaca ccacctggtc ggcgcccccc
     2341 aggacccagg cgcccagcag ccggttcgcc gcggcgtgca ccgcccgcag aagaggattg
     2401 cgatagggca catggccgat gtgctgcgtt acgacgaccg ggcgacgtcg gccgaagcgc
     2461 gcggcggcaa aggcggccag cacaggcagg tacaggcaat cgtgcaggtg cacgacgttc
     2521 gccgagcgga ccgagcgcgc caggcgcaac aaggcagcgg gtgaccagaa aggatagggc
     2581 acgcccgccg cacgct
//
LOCUS       DASRIE010000178         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1997457, whole genome shotgun sequence.
ACCESSION   DASRIE010000178 DASRIE010000000
VERSION     DASRIE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997457"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFB53_07440"
     CDS             <1..218
                     /locus_tag="VFB53_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86601.1"
                     /translation="AAAHAMAHETVLEVLREALRRARREGRREGLGRLLHSLEGWFAE
                     HARTGDAAFARHMAQAGHVGRSVRGAG"
     gene            283..891
                     /gene="gstA"
                     /locus_tag="VFB53_07445"
     CDS             283..891
                     /gene="gstA"
                     /locus_tag="VFB53_07445"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HZN86602.1"
                     /translation="MKLYYSPGACSLSPHIVLRESGLPFELVLASTKTHALQDGTDYY
                     TINPKGYVPLLELDDGQRLTEGPAIVQYIADRVPGKKLAPPNGTMERYRLQEWLNFVS
                     SEIHKSFSPLFNPAMPQDAKAAFKERVVKRLAWVDTQLQGRAYLMGDDFTAADAYLFV
                     TTNWSKPMAIDMSAWPHLAAFRERVGARPAVQEAMKAEGLVK"
     gene            complement(971..1936)
                     /locus_tag="VFB53_07450"
     CDS             complement(971..1936)
                     /locus_tag="VFB53_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN86603.1"
                     /translation="MRRTLPSTAALAAFESAARHQSFTKAADELSVTQSAVCRQIGAL
                     EEFVGVKLFRRSSRGVMLTEAGLAYSRQVTARLDEVERDTLDLMAKGGRGGTLELGVV
                     PTFGTRWLLPRLPQFAARHPGITVNLTARTRPFLFDDTPFDAAIGVGESAWPGTEGVF
                     LMHEQMVPIVHPGYLRRHRGARGEADWARCTLLQQSTRPYAWRHWFGSRGVPVDGDMT
                     GPRFELFSMLIEAAIHEMGVALVPRMLVEAELARGSLVLASRHESLSDRSYHLVYPAR
                     KSEHHALAPFRDWLQHEAKVYREAHGLGAPARPHAPAQDAGERGE"
     gene            2036..>2307
                     /locus_tag="VFB53_07455"
     CDS             2036..>2307
                     /locus_tag="VFB53_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN86604.1"
                     /translation="MSSSHKYAAFQWDDPLLLGPQLTEDERMVADSARAYCQERLAPR
                     VLEAFRHERTDPAIFREMGELGLLGATIPEAYGGAGLNDVCYGLIAR"
BASE COUNT          333 a          823 c          839 g          312 t
ORIGIN      
        1 aggccgcggc ccatgcgatg gcgcacgaga ccgtgctcga ggtgctgcgc gaggcgctgc
       61 gccgcgcgcg ccgggagggc cggcgcgagg gcctgggccg gctgctgcac agcctcgaag
      121 gctggttcgc cgagcacgcg cgcaccgggg atgcggcttt cgcgcggcac atggcgcagg
      181 ccggccacgt ggggcgctcg gtgcgcggcg ccggctgacg tcgccgcacg gggcgcgctc
      241 aggcgcgcta cattgcgcag cctgttccac cacgaggtcc gcatgaagct ctactacagc
      301 cccggcgcct gctcgctgtc gccgcacatc gtcctgcgcg aatccggcct gccgttcgag
      361 ctggtgctcg cgagcaccaa gacgcacgcc ctgcaggacg gaacggacta ctacaccatc
      421 aaccccaagg gctacgtgcc gctgctcgag ctggacgacg gccagcgcct caccgagggg
      481 ccggcgatcg tgcagtacat cgccgaccgc gttcccggca agaagctcgc gccgccgaac
      541 ggcaccatgg aacgctaccg gctgcaggaa tggctgaact tcgtctcctc cgagatccac
      601 aagagcttca gcccgctgtt caacccggcg atgccgcaag acgcgaaggc ggcgttcaag
      661 gagcgcgtcg tgaagcgcct ggcgtgggtc gacacgcagc tgcaggggcg cgcctacctg
      721 atgggcgacg acttcaccgc cgccgacgct tacctgttcg tgacgacgaa ctggagcaag
      781 ccgatggcca tcgacatgag cgcctggccg catctcgcgg ccttccgcga gcgcgtgggc
      841 gcgcgcccgg cggtgcagga ggcgatgaag gccgaagggc tggtgaagta gggcggcgac
      901 gcgcgcggcg ccggcgccga ggccggggcg tcgcgcctgc gcggcgacgc gccgcaggcg
      961 gctcgcccgc tcattcgcca cgctccccgg cgtcctgcgc cggcgcgtgc gggcgggccg
     1021 gcgcgcccag cccgtgcgcc tcgcggtaca ccttcgcctc gtgctgcagc cagtcgcgaa
     1081 acggcgccag cgcatggtgt tcggacttgc gcgcggggta gaccaggtgg tagctgcggt
     1141 cgctcaggct ctcgtgcctg gaggccagca cgagcgagcc ccgggcgagc tccgcttcga
     1201 ccagcatgcg cggcaccagc gccacgccca tctcgtggat ggccgcctcg atcagcatcg
     1261 agaacagctc gaagcgcggg cccgtcatgt cgccgtccac cggcacgccg cgcgagccga
     1321 accagtgccg ccaggcgtac ggccgcgtgc tctgctgcag cagcgtgcag cgcgcccagt
     1381 cggcctcgcc gcgcgcgccg cgatggcgcc gcaggtagcc ggggtggacg atcggcacca
     1441 tctgctcgtg catcaggaac acgccctcgg tcccgggcca ggcgctttcg cccacgccga
     1501 tggccgcatc gaacggcgtg tcgtcgaaca ggaacgggcg cgtgcgggcc gtgaggttga
     1561 ccgtgatgcc ggggtgccgg gcggcgaact gcggcagccg cggcagcagc caccgcgtgc
     1621 cgaacgtggg cacgaccccc agctccagcg tgccgccgcg gccgcccttg gccatgaggt
     1681 ccagcgtgtc gcgctcgact tcgtcgagcc gggcggtgac ctgccggctg taggcgagcc
     1741 cggcctcggt cagcatgacg ccgcggctgc tgcgccggaa cagcttcacg ccgacgaact
     1801 cctccagcgc gccgatctgc cggcacacgg cgctctgggt gaccgacagc tcgtcggccg
     1861 ccttcgtgaa gctctggtgg cgcgcggccg attcgaacgc cgccagggcg gcggtggacg
     1921 gaagggtgcg tctcatgaag tgagttttcg gaacaggtga gttcgaataa gtcgcttgcg
     1981 tcccggggcg gcccctggcc aaatggcggc gcctggacga tcaaccgaga cccgaatgag
     2041 ttcttcgcac aagtatgccg cgttccagtg ggacgacccg ctgctgctcg gcccgcagct
     2101 caccgaggac gagcgcatgg tggccgactc cgcgcgcgcc tactgccagg agcggctggc
     2161 gccgcgcgtg ctggaggcct tccgccacga gcgcaccgat ccggcgatct ttcgcgagat
     2221 gggcgagctg ggcttgctcg gggcgaccat tcccgaggcg tacggcggcg cggggctgaa
     2281 cgacgtgtgc tacgggctga tcgcgcg
//
LOCUS       DASRIE010000179         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3574621, whole genome shotgun sequence.
ACCESSION   DASRIE010000179 DASRIE010000000
VERSION     DASRIE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574621"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFB53_07460"
     CDS             complement(<1..382)
                     /locus_tag="VFB53_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZN86605.1"
                     /translation="MSGAADYLRFFVTLTAVVDPFLAVPFFLAFTAARSDAERKRLAR
                     VVAFTVFLVLAISVFVGEALLNFIGASLPAFRVGGGLVLLLMALAMLNAQAGGVRQSH
                     AEARELEQGELQGVVPLAMPLLAGP"
     gene            complement(379..1023)
                     /locus_tag="VFB53_07465"
     CDS             complement(379..1023)
                     /locus_tag="VFB53_07465"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZN86606.1"
                     /translation="MIRLCGFRISNYHNKVRIALLEKGVPFEEDDSVHGSQKPEYLAK
                     SPMGKVPYLEVDGQLLRESEVILEYLEDAFPQKPLLPKDPLERARVRELVTFIELHME
                     LVVRRLYNGLFFGGSFSEETKAAVEKDIAKGVRALKAVAKFDPYIAGKDLTLADCAAF
                     VHLPIVTLVTRTAFGRDFLEDIPPMKPYLKMLGERPAFAKVNEERKAASAAAKK"
     gene            complement(1020..1790)
                     /locus_tag="VFB53_07470"
     CDS             complement(1020..1790)
                     /locus_tag="VFB53_07470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86607.1"
                     /translation="MDLKGKVAVITGAASGIGRAAARRFARAGAAGLVLADLNMAGLK
                     PVAEELGGLAVACDVSREADIKRLVLQAEEKHGRIDVYFSNAGIVQRGGLAASDADWD
                     RNWRIHVMAHVWAARATVEKMVARGGGYFLVTASAAGLLNIVESATYGVTKHGAVAFA
                     EWLAIAYGRRGLKVSCLCPQAVITGMYKGDGGSAGVDGALSAEQVAEEVVKAMAAEKF
                     LVLPHPTVLDYMRNKTADYDRWIGGMQKLYAKHQGERK"
     gene            complement(1792..>2263)
                     /locus_tag="VFB53_07475"
     CDS             complement(1792..>2263)
                     /locus_tag="VFB53_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN86608.1"
                     /translation="GPGRIHHCMRLIGQAERALEKMCKRATQRVAFGRPVADQGVTRE
                     RIAEARIMIDSARFMVLNAAWKMDTVGNKVAQKEIAMIKVLAPTMACQVIDWAMQVHG
                     GGGVTDQFGLANMYAHARTLRFADGPDEVHRNAIAKLEIAEQTTLAQALAGRKH"
BASE COUNT          321 a          828 c          748 g          366 t
ORIGIN      
        1 ccgggcccgc gagcagcggc atggcgagcg gcaccacgcc ctgcagctcc ccctgctcca
       61 gctccctcgc ctcggcgtgc gactggcgca ccccgcccgc ctgggcgttc agcatcgcga
      121 gcgccatcag cagcagcacc aggccgccgc cgacgcggaa cgccggcagg ctcgcgccga
      181 tgaagttgag cagcgcctcg ccgacgaaca ccgagatggc gagcaccagg aacacggtga
      241 acgccaccac ccgcgcgagg cgcttgcgct cggcgtcgct gcgcgcggcg gtgaaggcga
      301 ggaaaaaggg gaccgcgagg aacggatcga cgaccgcggt cagcgtgacg aagaagcgca
      361 ggtagtcggc ggcgccgctc actttttcgc cgcggcgctc gccgccttgc gctcttcgtt
      421 caccttggcg aaggcggggc gctcgcccag catcttcagg tagggcttca tcggcgggat
      481 gtcctcgagg aagtcgcgcc cgaaggcggt cctggtcacc agcgtgacga tgggcaggtg
      541 cacgaaggcc gcgcagtcgg ccagcgtcag atccttgccg gcgatgtagg gatcgaactt
      601 cgccaccgcc ttgagggcgc gcacgccctt ggcgatgtcc ttctccaccg cggccttcgt
      661 ttcctccgag aagctgccgc cgaagaacag gccgttgtac aggcgccgca ccaccagctc
      721 catgtgcagc tcgatgaagg tcaccagctc gcgcacgcgc gcgcgctcga gcggatcctt
      781 cggcagcagg ggcttctgcg gaaaggcgtc ctccaggtat tcgaggatca cttccgactc
      841 gcgcagcagc tgcccgtcga cttccaggta gggcaccttg cccatcgggc tcttcgccag
      901 gtactcgggc ttctgcgagc cgtgcacgct gtcgtcttcc tcgaacggca cgcccttctc
      961 cagcagcgcg atgcgcacct tgttgtggta gttgctgatg cgaaagccgc aaaggcggat
     1021 catttcctct ccccctggtg tttcgcgtag agcttctgca tgccgccgat ccagcggtcg
     1081 tagtcggcgg tcttgttgcg catgtagtcg agcaccgtgg ggtgcggcag gacgaggaat
     1141 ttctccgcgg ccatcgcctt caccacctcc tcggccacct gctccgcgct gagcgccccg
     1201 tccacgccgg ccgagccgcc gtcgcccttg tacatgccgg tgatcaccgc ctgcgggcac
     1261 aggcaggaca ccttgaggcc gcgccgcccg taggcgatcg ccagccactc ggcgaaagcc
     1321 accgcgccgt gcttggtgac gccgtaggtg gccgattcga cgatgttgag cagtcccgcg
     1381 gccgaggcgg tgacgaggaa gtagccgccg ccgcgcgcca ccatcttctc caccgtcgcg
     1441 cgcgcggccc acacgtgcgc catgacgtgg atgcgccagt tgcggtccca gtcggcgtcg
     1501 ctcgccgcca ggccgccgcg ctgcacgatg ccggcgttgg aaaaatacac gtcgatgcgc
     1561 ccgtgcttct cctcggcctg cagcaccagg cgcttgatgt ccgcctcgcg gctcacgtcg
     1621 caggccaccg ccaggccgcc gagctcctcc gccaccggct tgaggcccgc catgttgagg
     1681 tcggcgagca ccaggccggc cgcgcccgcc cgcgcgaagc gccgcgccgc ggcgcggccg
     1741 atgccgctcg ccgcgccggt gatcaccgcc accttgccct tgaggtccat gtcaatgctt
     1801 gcggccggcg agcgcctggg cgagcgtggt ctgctcggcg atttcgagct tggcgatggc
     1861 gttgcggtgc acttcgtccg ggccgtcggc gaacctgagc gtgcgcgcgt gcgcgtacat
     1921 gttggcgagg ccgaactggt cggtgacccc gccgccgccg tgcacctgca tcgcccagtc
     1981 gatcacctgg caggccatgg tcggcgccag caccttgatc atggcgatct ccttctgcgc
     2041 caccttgttg cccaccgtgt ccatcttcca ggcggcgttg agcaccatga agcgcgccga
     2101 gtcgatcatg atgcgcgcct cggcgatgcg ctcgcgcgtc acgccctggt cggcgaccgg
     2161 ccggccgaac gccacgcgtt gcgtcgcgcg cttgcacatc ttctccagcg cgcgctccgc
     2221 ctggccgatg aggcgcatgc agtggtggat gcgccccggc ccg
//
LOCUS       DASRIE010000180        20048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1233918, whole genome shotgun sequence.
ACCESSION   DASRIE010000180 DASRIE010000000
VERSION     DASRIE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20048
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233918"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFB53_07480"
     CDS             complement(<1..646)
                     /locus_tag="VFB53_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HZN86609.1"
                     /translation="MKAFVVGGAVRDELLGLAVADRDWVVVGATPEDMANQGYKPVGK
                     DFPVFLHPETHEEYALARTERKSGRGYKGFTVHAAPDVTLEQDLERRDLTINAMAKDE
                     AGRLVDPYGGEKDLREGVLRHVSAAFAEDPVRILRVARFTARFGFRIAEETLELMQAM
                     VASGEADHLVKERVWQEFARGLAEPNPKLMFQALESCGLRAKLLPELERVPRHFD"
     gene            complement(643..1269)
                     /locus_tag="VFB53_07485"
     CDS             complement(643..1269)
                     /locus_tag="VFB53_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZN86610.1"
                     /translation="MLLVIGNKNYSSWSLRPWLAMTVLGIPFQEQRIPLYVEGAKEKL
                     RAYSPAGKVPCLVDGEQRVWDSLAILETLAERHPGVWPADPAQRARARSISAEMHSGF
                     ANLRNHMSMNIRKRYPGKGRTPEVLAEIARVVEIWSERRGPFLFGAFTAADAMYAPVV
                     LRFRTYEVELPPVCRAYADAVLALPALQEWIADAEREAESLPQFEQYA"
     gene            complement(1279..2253)
                     /locus_tag="VFB53_07490"
     CDS             complement(1279..2253)
                     /locus_tag="VFB53_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HZN86611.1"
                     /translation="MTRSRDVLVVGGSGFVGRHLVAALAARGVNVTVPTRRRERAKHL
                     LMLPTVELVEADVHRPAALARLADGKDAVVNLVGVLHSRRGRPDERGPNDYGPDFAHV
                     HVELAQAVVRACRSAGVKRLLHMSALGAAPDAPSEYLRSKGIGERAALAAEDLAVTVF
                     RPSVIFGPEDAFLNLFAALTRFLPVVALACPGARFQPVYVGDVAAAFVAALEDRASFG
                     RRYDLGGPRQYTLRELVEAVCAITHRRRMIVGLGPRLSMLQARMLELLPVPPMTRDNV
                     RSMQLPNVCAAAFPFGIEPAALETLAPAWLAPVGPRVRYPDLRSHARR"
     gene            complement(2250..4178)
                     /locus_tag="VFB53_07495"
     CDS             complement(2250..4178)
                     /locus_tag="VFB53_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZN86612.1"
                     /translation="MIYRRFLTIALGCATFATGSASGNDEAVLGANAAFRAGDPDKLA
                     RHAAALKGHTLEPWAEYWRIRLRIEEARPAEVRGYLARHAGTYLADLLRADWLKELGR
                     RGAWQDFEHDLGPLVQDDLEIQCHAWSARLARGDESAAAEARAAWLEPRELPDACLPL
                     TERMAKNGGPSAERAWQRARRLLAASQLSAARRTLGALPAAEQPDERLLSQAATAPKR
                     LLSQPPRDLERRAVREMVLFALTRLARSDARAAAEVLEGALGERLPEADRAYLWGQVA
                     AQASYELDADALAWFARAGATPLSDEQLAWKARAALRAGDWQVLREAVAPMSPVARQD
                     MAWTYWTGRALAAQGDAEGARAQFARIAGQPLYYSLLATEELGGEAGIVLPPREPTEK
                     EVDDARRNVELARALELFRLGLRTEATSEWIFAVRNLDDQRLLAAAELARRAAVYDRA
                     INTAGRTERLHDYRVRYLAPFRDVFREYARAYDLEEAWVLGLVRQESRFIVDARSGAG
                     ATGLMQLLPHTARWVAQRIGYKGYNLKRVAEVETNVTLGTRYLRHVLDGTGHPVLASA
                     AYNAGPNRARRWQPAQAMEGAVFVESIPYGETREYVKRVLTNTVHYALLLEGRSVPLK
                     RRLGTIPGRDAAAIESMQ"
     gene            4200..4868
                     /locus_tag="VFB53_07500"
     CDS             4200..4868
                     /locus_tag="VFB53_07500"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZN86613.1"
                     /translation="MRPEELKAWRKTERQRLLAAREALDPRAVEGFRRRIDAHLERGF
                     PGLKAATFAFCWPIRNEYDARHLAKTLRAAGALTALPVVVAPKTPLAFREWHPGIALA
                     RGPLDIPYPADSRELAPKAVLLPMNGWDAQGYRLGYGGGFFDRTLASLREKPLAIGVS
                     YELARMPTIHPQAWDIPMDYVVTERGVYRRDGGALAFLGEPGPASELASPVCYADERI
                     LRGD"
     gene            complement(4865..6178)
                     /locus_tag="VFB53_07505"
     CDS             complement(4865..6178)
                     /locus_tag="VFB53_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86614.1"
                     /translation="MHLRASRFGFVLALFLSLAAGSAGAADRTQVFDDAVAAFRAGDY
                     DAALRSFLVARSLGLDTPGLRYNLGVTYFRLQRYAEAQAAFEALSRDPMWSALAHYNL
                     GLVAQRMGDLRQARTHFDLAHRATTDPKLRTLAGVALERVDAATLPPRPGTLVSLGGG
                     YDSNPAVYTENTAAGGGGDYFGELLAVATRNLSGNTALGWNGHGALIWRKYLDQGEFD
                     LQGLRAGISRDTDSANTQFSLGGNYGLVYFDGKLLEQAASLELQARTRLDAGRDLRGR
                     YHLARVDGGGHYRQLDGWQHRASADAGFGWGRALWRAGLQFEIEDRRDLAQGGEFLSY
                     SPKRQLLFATAELPDVAGWQADLRLEYRASRYRDPYRLDGGTRVVERTDDRTSVALRG
                     SRGAGAGRRVFVEFSYYRNASSIDQYDYRRGQLLAGIELDLEKAP"
     gene            complement(6225..6950)
                     /locus_tag="VFB53_07510"
     CDS             complement(6225..6950)
                     /locus_tag="VFB53_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZN86615.1"
                     /translation="MRVAAASADTRPAVRGQRLLAGSTVSTAAGSRAILSFDDGQIAA
                     LHENTQFRIDGFRYQLEKPQADRAVFVLLQGALRIVTGALGQRNPAAFHLRTGETTVG
                     IRGTDFMVVKVNPVYLSVLQGRIAATNSAGTTLFGAGDFGAVASGKSLAAAVRENALP
                     AGASSAFGRLGALPLAPPAPPGAGGVKVPASAVSQPQDPGAFGRETADKARALKDDLA
                     RKAKKAAAERARDPATRPGKGPQ"
     gene            complement(7127..7438)
                     /locus_tag="VFB53_07515"
     CDS             complement(7127..7438)
                     /locus_tag="VFB53_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86616.1"
                     /translation="MKRLTIIAAGAALALALPGLAWAQQRDLEVTMDVVPANAASDSA
                     TKVIELPATASDRARERSAFGHSVANKARESRGELGREFGQSVSEAARERGKPATPGK
                     P"
     gene            complement(7455..8063)
                     /locus_tag="VFB53_07520"
     CDS             complement(7455..8063)
                     /locus_tag="VFB53_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN86617.1"
                     /translation="MDCRNFALRVDDWLDGRLGAAEQEPLRLHLQSCEGCRRGHADAE
                     SLRAEMRKLAAPAMRPGFAAQALAKAVAAPAGAAPAMALAASLALGVGVAAILFAVRP
                     EPAPTVSLSVQQPESIHLVFNSAKALQGATLSLWLPENVQIVGYGDRRELTWSTDLRE
                     GANLLQLPLVLQARANGEFVAVLSHGESSKRFRLKIEARPQG"
     gene            complement(8075..8659)
                     /locus_tag="VFB53_07525"
     CDS             complement(8075..8659)
                     /locus_tag="VFB53_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN86618.1"
                     /translation="MVRVLPFGLTRIAKASFEALLRPHVEFLHRLAWRFTGSTADAED
                     LVQDVLLKLYARSRQLAAVEQLRPWLAKVLYRTYVDFVRRQARSPASGLADDAGGDEA
                     RLDAFPAAEDGPPELAERGELRERVLAALRRLNPEQRAVVVMHDIEGYALEELEGILQ
                     APLGTLKSRLHRARQQLRAHLGMEPFAASERVKR"
     gene            complement(8737..9399)
                     /locus_tag="VFB53_07530"
     CDS             complement(8737..9399)
                     /locus_tag="VFB53_07530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86619.1"
                     /translation="MTARTLPLDERLQRYVLEHGARETAVQRELRSVTQRLPQSSMQI
                     GAEQGALMQMLVRVMGARRCLEIGTFTGYSALAVALALPPAGRVICCDVSEEWTAIAR
                     RYWKRAGVAGKIELRLAPALRTLDALLEEGRAGRFDFAFIDADKANYQNYYERCLRLV
                     RRGGLIAVDNTLWGGSVADPRKKSVDTLAIRAFNRQLVRDRRVEIALVPVGDGLTLAQ
                     KN"
     gene            complement(9396..10910)
                     /gene="ilvA"
                     /locus_tag="VFB53_07535"
     CDS             complement(9396..10910)
                     /gene="ilvA"
                     /locus_tag="VFB53_07535"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZN86620.1"
                     /translation="MKREDYLERILRARVYDVAIESPLELAPALSRRLHNRLLLKRED
                     LQPVFSFKLRGAYNKMANLPRRRLRQGVIAASAGNHAQGVALAAQKLGCRAVIVMPVT
                     APKIKVDAVAARGAKVVLHGDSYDEAHAHAMQLKRRQGLSFVHPYDDPDVIAGQGTIG
                     MEILRQSEKPIDAVFVAVGGGGLISGIASYLKHLRPGIRVIGVEPADADAMTRSLRAG
                     RRVKLDHVGLFADGVAVKEVGRETFRLCRKLVDGMVRVDTDEMCAAIKDVFEDTRVVL
                     EPAGALAIAGAKAWVAKTGARGKTLVAVASGANTNFDRLRFVAEQAELGEHREAVLAV
                     TIPERRGSFRRFLEALGNRNVTEFNYRMSGPSAAHIFVGVSVAGRAETARLARRLARA
                     GCRTLDLSDNEIAKAHVRHMVGGPAALARHELLYRFEFPERQGALMKFLRAMNPSWNI
                     SLFHYRNHGADYGSVLVGIEVPPREMGDFRRFLARLGYPWADETRNPAYRLFLK"
     gene            10936..11343
                     /locus_tag="VFB53_07540"
     CDS             10936..11343
                     /locus_tag="VFB53_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZN86621.1"
                     /translation="MDTLLSPEGGLAGVLVAGFLAATLVPFSSEAVLFGYLKLHPGNA
                     GIAVALATLGNTAGGMTSYLLGRFLPRKELPHLELVRKYGAPALLFAWLPLVGDAFCV
                     AAGWLRVHWLAALVCMAAGRLARYLAVAALAPS"
     gene            11397..12371
                     /locus_tag="VFB53_07545"
     CDS             11397..12371
                     /locus_tag="VFB53_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN86622.1"
                     /translation="MRTWLIAAALAAAAPAHAQIVKVDGSSTVFPVTEAVAEDFQKAG
                     KGAVKVTVGISGTGGGFKKFCRGETDVSNASRPILRKEMEDCKAAGIEYIELPVAFDA
                     LTVVVHPKNSFLKQLTVAEMKKMWEPGAQGKVMRWNQVNAAWPDAPMKLFGPGADSGT
                     FDYFTEAVVGKSKSSRGDFTASEDDNVLVQGVSRDANALGYFGFAYYVENKDKLKAVP
                     IVNEKGQAVLPSLEAVEKGSYTPLARPIFIYVSTKALAKPEVKQFVQYYMTHGAKLAK
                     EVKYVPLPASAYQTAWDHVQKGKKGTVFGGVAEVGVTIEELLRREARL"
     gene            12428..13294
                     /gene="pstC"
                     /locus_tag="VFB53_07550"
     CDS             12428..13294
                     /gene="pstC"
                     /locus_tag="VFB53_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZN86623.1"
                     /translation="MRERAIEAVLFAAALVSVFTTVGIVYILVKESVVFFQQVPIVDF
                     LTDRQWTPLFDDAHFGIMVLLSGTLTSSLVALSIAIPLGTVIAIYLSEFAPFKLREIA
                     KPFLELLGGVPTIVYGYFALLFVTPLLQKIYPGLPGFNLLAAGLVMGVMIIPYVASIS
                     EDAMRAVPMALREGSYATGATRLQTALRVVFPAAFSGVAAAYILGISRAVGETMILAV
                     AAGMQPNLTFNPLEPAATITAYIVQVALGDLPHGSVGYQTIFAAGLTLMLITLVFNIA
                     GHMLRKRFREAY"
     gene            13291..14190
                     /gene="pstA"
                     /locus_tag="VFB53_07555"
     CDS             13291..14190
                     /gene="pstA"
                     /locus_tag="VFB53_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZN86624.1"
                     /translation="MKDNLWRRKRYEAIFAVVGLLALMIGVLTFATLFAEMAINGMGR
                     LDWSFFTSFPSRRPGEAGILSAWIGSTLVMVVTAFFAVPLGVAAGVYLEEYARKNWVT
                     DLIEINITNLAGVPSIVYGLLALGLFVYQFGLGQSILAAGLTLALLILPVVIVATREA
                     IRSIPQAIREGSYALGATQWQTVRYHILPYSSAGILTGVIIGMARAIGETAPIITIGA
                     LTFIAFLPPSPVQAAPPFLSFEWLLSPFTVMPIQMFNWTSRPEAAFQVNAAAAGFVLV
                     IMTLAMNGLAIWLRYRLRRNIKW"
     gene            14237..15019
                     /gene="pstB"
                     /locus_tag="VFB53_07560"
     CDS             14237..15019
                     /gene="pstB"
                     /locus_tag="VFB53_07560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZN86625.1"
                     /translation="MRERKVALAPKAQVSGLDFYYGDFRALKSINLTVYEQQVTALIG
                     PSGCGKSTFLRCFNRMHDLYPGNRYAGSIVLHPDNTDLLSREVDPIEVRMRIGMVFQK
                     PNPFPKSIFENVAYGLRVRGEVSRRRMAEKVEQALESAALLDEVKDRLHQPAYNLSGG
                     QQQRLCIARALATDPELLLFDEPTSALDPIATASIEELIHDLKKQVTILIVTHNMQQA
                     ARVSDYTAYMYLGELIETGVTDELFIKPRMKQTEDYITGRFG"
     gene            15016..15705
                     /gene="phoU"
                     /locus_tag="VFB53_07565"
     CDS             15016..15705
                     /gene="phoU"
                     /locus_tag="VFB53_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZN86626.1"
                     /translation="MTEHASKQYDLDLDSIRSRVLQMGGLVESQIRRALEAFGSGERA
                     LIEQVIAEDHRVNAMEVALDGDCSQVIVKRQPAAGDLRMIFAITKTVTDLERIGDEAR
                     KIARMARSIHERGAQSVPLAVDVRHAAELAIAMLQRSLDAFARLDADAAAEVIAADAG
                     IDSEFRSILRQLITYMMEDPRTISMALEIVWIAKAVERIGDHAKNMAEYVIYIVKGTD
                     VRHTNPEQLEA"
     gene            15702..16388
                     /gene="phoB"
                     /locus_tag="VFB53_07570"
     CDS             15702..16388
                     /gene="phoB"
                     /locus_tag="VFB53_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZN86627.1"
                     /translation="MSATVLVVEDEPQIRELVVVNLEHAGHRVLRAGNAEQAEQSIRG
                     ELPDVVVLDWMLPGQSGLDFARRLRADPRTRELPILMLTARAMEADKLAGLEAGADDY
                     LTKPFSPRELIARIKAVLRRRAPQYAGDPVEIAGLRLDPAAHLVSAGEQRLELSPVEY
                     RLLQFFMTHPGRVYSRGQLLDQVWGDHVYLEERTVDVHIRRLRKALEPSGHDRLIDTV
                     RGSGYALRPA"
     gene            16385..17749
                     /gene="phoR"
                     /locus_tag="VFB53_07575"
     CDS             16385..17749
                     /gene="phoR"
                     /locus_tag="VFB53_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HZN86628.1"
                     /translation="MRRIARGAVVPFAALALLAGLAGAAGGAAWGWGALAAGLAALLA
                     HHLLNLARLAEWLSGHRPGEVPEGSGSWFEVFSALHRQERSAHQRGALLAEALARFRR
                     AVQALPDGVVILDAESRIEWCNATAEAQLGLDARADLGQPIANLVREPAFVAYLGAGE
                     DAAPLRVEPRPGRALVLQLIPYGGTERLLLSRDVTQAERLETMRRDFVANVSHELRTP
                     LTVLSGFLETLRELKLDPQRQRDYLDMMREQSARMQGIIGDLLTLSTLESAPPPAAAR
                     VRVAPLLERLHADALGLSGGRHEISLQGRPAVDLAGSEAELASAFGNLVSNAVRYTPR
                     GGKVTLLWSDDALGAQFAVADTGVGVAPEHIPRLTERFYRVDRGRSRESGGTGLGLAI
                     VKHALARHQATLHIESTPGAGSRFAVRFPAQRTLPLTAEVDAMPPAASPPPPPATSRP
                     AGRN"
     gene            complement(17685..19868)
                     /locus_tag="VFB53_07580"
     CDS             complement(17685..19868)
                     /locus_tag="VFB53_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN86629.1"
                     /translation="MSLHLEHLNPEQLAAVTLPEGHALILAGAGSGKTRVLTTRIAWL
                     IKEGSVSPSGVLAVTFTNKAAREMLTRLGTMLPANPRGMWIGTFHGLCNRMLRAHHQE
                     AGLPRTFQILDSQDQLAAVKRLLRTMNVDEDRFPPRDVQYFIMGKKEEGLRARDVPLT
                     DDNTRRFVEFYAAYDEQCQREGVVDFAELLLRSFELLSRNEPLREHYRARFRHILVDE
                     FQDTNKLQYRWLKLLAGPLTSLFAVGDDDQSIDAFRGANVGNMADYERDYKVAKVVRL
                     EQNYRSQGNILDAANALIANNKNRLGKNLWTAAGAGEPLRVFEGQSDVDEARWMVEEI
                     KALQREGSRLDQIAVVYRSNAQSRVLEHALFTAALPYRVYGGLRFFERAEVKHALAYL
                     RLVAAPADDNAFLRVVNFPSRGIGARTVEALQDAAKAAGTSLYETALAAPGKSAPFIA
                     LIESLREETQGLTLAETVEQALAKSRLIEHYKTEREGADRIENLNELVNAAAAFSLQD
                     AAVESGEEADPLTAFLTHAALEAGEHQAGEGQDALQLMTMHSAKGLEFNAVFVSGLEE
                     GLFPHENSLLEKDGPEEERRLMYVALTRARKRLYLSFAQTRMLHGQTRYNLPSRFLDE
                     IPQGLLKWLTPRFAQKPKPAVSHFTAGSFTRAPKRDPRFGGAPAGTGGAGGAFRIGQN
                     VNHAKFGSGVIVDAEGQGSDARVQVNFGRQGVKWLAVAVAKLQAA"
BASE COUNT         2902 a         7174 c         6968 g         3004 t
ORIGIN      
        1 cgtcgaagtg tcgcggcacc ctctccagct cgggcaggag cttcgcgcgc agcccgcagc
       61 tctccagcgc ctggaacatg agcttcggat tcggctcggc gagcccgcgc gcgaactcct
      121 gccagacgcg ctccttcacc aggtggtcgg cctcgcccga ggcgaccatc gcctgcatca
      181 actccagcgt ctcctcggcg atgcgaaagc cgaagcgcgc ggtgaagcgc gccacgcgca
      241 ggatgcgcac cgggtcctcc gcgaaagccg ccgagacgtg ccgcagcacg ccttcgcgca
      301 ggtccttctc gccgccgtag ggatcgacca ggcgcccggc ctcgtccttc gccatggcgt
      361 tgatggtgag gtcgcgccgc tcgaggtcct gctcgagggt aacgtcggga gcggcgtgca
      421 cggtgaaccc cttgtagccg cggccgctct ttcgctcggt gcgcgcgagc gcgtactcct
      481 cgtgcgtctc cggatgcagg aacaccggga aatccttgcc cacgggcttg tagccctggt
      541 tcgccatgtc ctccggcgtg gcgccgacca ccacccagtc gcggtcggcc accgcgaggc
      601 cgagcagctc gtcgcgcacg gcgccgccga cgacgaaggc cttcacgcgt actgttcgaa
      661 ctgcgggagc gactccgcct cgcgctccgc gtcggcgatc cattcctgca gcgcgggcag
      721 cgcgagcacc gcgtcggcgt aggcgcgaca gacgggcggc agctcgactt cgtaggtgcg
      781 aaagcgcagc accaccggcg cgtacatggc atcggcggcg gtgaaagcgc cgaacaggaa
      841 cgggccgcgc cgttcgctcc agatctcgac gacgcgcgcg atctccgcca gcacctccgg
      901 cgtgcgcccc ttgcccgggt agcgcttgcg gatgttcatg ctcatgtggt tgcgcaggtt
      961 cgcaaagccc gagtgcatct ccgcgctgat cgaacgcgcg cgcgcgcgct gcgcagggtc
     1021 ggcgggccac accccgggat ggcgctcggc gagggtctcc aggatggcga gcgaatccca
     1081 cacccgctgc tcgccgtcca ccaggcaagg caccttgccc gccggggaat aggcgcgcag
     1141 cttctccttc gcgccctcga cgtacagcgg gatgcgctgc tcctggaacg ggatgccgag
     1201 caccgtcatc gccagccagg ggcgcagcga ccaggacgag tagttcttgt tgccgatgac
     1261 cagcagcatg cggggccttc agcgcctggc gtggctgcgc aggtccgggt agcgcacgcg
     1321 cggcccgacg ggagcgagcc acgcgggcgc gagcgtctcc agcgccgccg gctcgatgcc
     1381 gaacgggaac gccgcggcgc agacattggg cagctgcatc gagcgaacgt tgtcgcgcgt
     1441 catcggcggg accggcagca gctcgagcat gcgcgcctgg agcatcgaca ggcgcggccc
     1501 caggccgacg atcatgcggc gccggtgcgt gatggcgcag accgcctcga ccagctcgcg
     1561 cagggtgtac tgcctcgggc cgccgagatc gtagcgcctg ccgaacgagg cgcgatcctc
     1621 cagcgccgcg acgaacgccg ccgcgacgtc gccgacgtac accggctgga aacgcgcccc
     1681 ggggcaggcg agcgccacca ccggcaggaa gcgcgtcagc gccgcgaaca ggttgaggaa
     1741 ggcgtcctcc gggccgaaga tcaccgaggg gcggaacacc gtcaccgcca ggtcctccgc
     1801 ggcgagcgcc gcgcgctcgc cgatgccctt ggagcgcagg tactcggaag gcgcatcggg
     1861 cgcggcgccg agcgcgctca tgtgcagcag gcgcttcacg ccggcgctgc ggcaggcgcg
     1921 caccaccgcc tgcgccagtt ccacgtgcac gtgagcgaaa tccgggccgt agtcgttggg
     1981 cccgcgctcg tcgggccgcc cgcgccggct gtgcagcacg ccgaccaggt tcaccaccgc
     2041 gtccttgccg tcggcgaggc gcgcgagcgc ggcgggccgg tgcacgtcgg cctcgaccag
     2101 ctcgacggtg ggcagcatga gcaggtgctt ggcgcgctcg cgccggcgcg tgggcacggt
     2161 gacattgacg ccgcgcgccg cgagcgccgc caccaggtgg cggccgacga agcccgagcc
     2221 gccaaccacc agaacgtcgc ggctgcgcgt cattgcatcg actcgattgc tgccgcgtcg
     2281 cggcccggga tggtacccag gcgccgcttg agcgggacgc tgcggccctc cagcagcagc
     2341 gcgtagtgca cggtgttggt cagcacacgc ttcacgtact cgcgcgtctc gccgtaggga
     2401 atggattcga cgaacaccgc gccctccatc gcctgcgccg gctgccagcg gcgcgcccgg
     2461 ttcggcccgg cgttgtaggc ggccgaggcg agcaccgggt ggccggtgcc gtcgagcacg
     2521 tgccgcaggt agcgcgtgcc cagggtcacg ttggtctcca cttcggcgac gcgcttcagg
     2581 ttgtagccct tgtagccgat gcgctgcgcc acccagcgcg cggtatgcgg cagcagctgc
     2641 atcaggccgg tggcgccggc ccccgagcgc gcatcgacga tgaagcggct ttcctggcgc
     2701 accaggccga gcacccaggc ctcctccagg tcgtaagccc tggcgtattc gcggaacacg
     2761 tcgcggaacg gggcgaggta gcgcacgcgg tagtcgtgca ggcgctcggt acggccggcg
     2821 gtgttgatgg cgcggtcgta caccgcggcg cggcgcgcca gctcggcggc ggcgagcagg
     2881 cgctggtcgt ccaggttgcg caccgcgaag atccattcgc tggtcgcctc ggtgcgcagc
     2941 ccgaggcgga acagctcgag cgcgcgcgcc agctccacgt tgcgccgcgc atcgtcaact
     3001 tccttctccg tcggctcgcg cggcggcagc acgatgcccg cctcgccgcc gagttcctcg
     3061 gtggcgagca ggctgtagta gagcggctgg ccggcgatgc gcgcgaactg cgcgcgcgcg
     3121 ccttcggcgt cgccctgcgc ggcgagcgcg cgcccggtcc agtaggtcca ggccatgtcc
     3181 tggcgcgcca cgggggacat cggcgcgacg gcctcgcgca ggacctgcca gtcgccggcg
     3241 cgcagtgcgg cgcgcgcctt ccaggcgagc tgctcgtcgc tcaacggcgt ggcgccggcg
     3301 cgcgcgaacc aggcgagcgc gtcggcgtcg agctcgtagg aggcctgcgc cgcgacctgc
     3361 ccccacaggt aggcacgatc cgcctccggc aggcgctcgc cgagcgcacc ctcgagcact
     3421 tccgccgcgg cgcgcgcgtc gctgcgcgca aggcgcgtca gcgcgaacag caccatctcg
     3481 cgcaccgcgc ggcgctccag gtcgcgcggc ggctgcgaca gcaggcgctt gggagcggtc
     3541 gccgcctgcg acagcaggcg ctcgtcgggc tgctccgcgg ccggcagggc gccgagcgtg
     3601 cgccgcgccg cgctcagctg cgaagccgcg agcaatctcc gcgcgcgctg ccaggcgcgc
     3661 tcggcggagg gaccgccgtt cttcgccatg cgctcggtga gcggcaggca ggcgtcgggc
     3721 agctcgcgcg gctcgagcca cgcggcgcgc gcctccgcag cggcgctctc gtcgccgcgc
     3781 gccaggcgcg cgctccaggc gtggcactgg atctccaggt cgtcctgcac cagcggcccg
     3841 aggtcgtgct cgaagtcctg ccacgcgccg cggcggccga gctccttcag ccagtcggcg
     3901 cgcagcaggt ccgcgagata agtgccggcg tggcgcgcga ggtagccgcg cacctcggcc
     3961 ggcctggcct cttcgatgcg cagccggatg cgccaatact ccgcccaggg ctcgagcgta
     4021 tgccccttca acgcagcggc atggcgcgcg agcttgtccg ggtcgccggc gcggaaagcg
     4081 gcgttggcgc cgagcaccgc ttcgtcgttt ccgctggccg aacccgtggc aaacgtcgcg
     4141 cagcccagtg ctatagtcag gaaacgccta taaatcatga actaagaata gcacgtggca
     4201 tgcgaccgga agagctgaag gcctggcgca agaccgagcg gcagcgcttg ctcgccgcgc
     4261 gggaggcgct cgatccgcgg gcagtcgagg gcttccgccg ccggatcgac gcgcacctgg
     4321 agcgcggctt tcccggcctg aaggcggcca ccttcgcgtt ctgctggccg atccgcaacg
     4381 agtacgacgc gcgccacctg gctaaaacgc tgcgcgcggc cggcgcgttg accgcgctgc
     4441 cggtggtggt ggcgccgaag acgccgctcg cgttccgcga gtggcacccg ggaatcgcgc
     4501 tcgccagggg cccgctcgac ataccgtacc cggcagactc gagggagctc gcgccgaagg
     4561 cggtgctgct gccgatgaac ggctgggacg cgcagggcta tcgcctgggc tacggcggcg
     4621 gattcttcga ccgcacgctg gcgagcctgc gggaaaaacc gctggcgatc ggcgtgagct
     4681 acgagctggc gcgcatgccg accatccatc cgcaagcctg ggacatcccg atggactacg
     4741 tggtgaccga gcgcggcgtg taccggcgcg acggcggcgc gctcgccttc ctcggcgagc
     4801 ccgggccggc cagcgaactt gcctcgcccg tgtgctacgc cgacgagcgc atcctgcggg
     4861 gcgactaggg ggctttttcc agatcgagct cgatgccggc gagcagctgg ccgcgacggt
     4921 agtcgtactg gtcgatgctc gatgcgttgc ggtagtagct gaattcgacg aacacgcgcc
     4981 gcccggcgcc cgcgccgcgg ctgccgcgca gcgcgacact ggtgcgatcg tcggtgcgct
     5041 cgaccacgcg ggttccgccg tccaggcgat acgggtcgcg gtagcggctg gcgcggtact
     5101 ccaggcgcag gtcggcttgc cagcccgcga cgtcgggcag ctccgcggtg gcaaagagca
     5161 gctgccgctt gggcgaatag ctgaggaact cgcccccttg cgcgaggtcg cggcggtcct
     5221 cgatctcgaa ctggaggccc gcgcgccaca gcgcgcgccc ccagccgaac cccgcgtccg
     5281 cagatgcccg atgctgccag ccgtcgagct gccggtaatg gccgccgccg tcgacgcgcg
     5341 ccaggtgata gcgcccgcgc aggtcgcggc cggcgtccag gcgggtgcgc gcctgcagct
     5401 cgagcgaagc ggcctgctcc agcagcttgc cgtcgaaata caccaggcca tagttgccgc
     5461 ccaggctgaa ttgcgtgttc gcggaatccg tgtcgcggct gatgccggcc cgcaggccct
     5521 gcaggtcgaa ctcgccctga tcgaggtact tgcgccaaat gagagcgccg tgcccattcc
     5581 agcccagggc ggtattgccg ctcaggttgc gggtcgccac ggcgagcaat tcgccgaaat
     5641 aatcgccgcc gccgccggcc gccgtattct ccgtgtacac cgccggattg ctgtcgtagc
     5701 cgcccccgag cgacaccagg gtgccggggc gcgggggcag ggtcgcggcg tcgacccgct
     5761 ccagcgcgac cccggccagc gtacgcagct tcgggtcggt ggtcgcgcgg tgggccaggt
     5821 cgaagtgcgt gcgcgcctgc ctgaggtccc ccatgcgctg cgccaccagc cccaggttgt
     5881 agtgcgcgag ggcgctccac atcgggtcgc gcgagagcgc ctcgaaggcg gcttgcgcct
     5941 ctgcgtagcg ctgcagccgg aaataggtga caccgaggtt gtagcgcagg ccgggcgtgt
     6001 cgaggccgag gctccgcgcg acgagaaacg agcgcagtgc cgcgtcgtaa tcgccggcgc
     6061 ggaacgcggc taccgcgtca tcgaaaacct gcgtgcggtc ggctgcgccg gcggatcccg
     6121 ccgcgaggct gaggaacagc gcaagtacga agccaaagcg ggacgcccgg aggtgcatgc
     6181 gcttcggcgc aagcataccc ggccggcggg gaaaactcga cgccctattg cggccccttg
     6241 cccgggcgcg ttgccgggtc gcgggcgcgc tcggccgccg ccttcttcgc cttgcgcgcg
     6301 agatcgtcct tcagcgcgcg cgccttgtcg gcggtctcgc gcccgaaggc cccggggtcc
     6361 tgcggctgcg acaccgcgct cgcgggaacc ttcacgccgc ccgcgccggg cggcgcgggc
     6421 ggcgcaaggg gcagcgcgcc gaggcggccg aacgccgacg aggccccggc aggcaaggcg
     6481 ttctcgcgca cggcggcggc aagcgacttg ccgctcgcga ccgcgccgaa atctccggcc
     6541 ccgaacagcg tggtgcccgc ggaattcgtc gccgcgatgc gcccctggag gacggacagg
     6601 tagacgggat tcaccttcac gaccatgaag tcggtgcccc ggatgccgac cgttgtttcc
     6661 ccggtgcgca gatggaaagc ggccggattg cgctgcccca gcgcgccggt gacgatgcgc
     6721 agggcgccct gcagcaggac gaacaccgcg cgatcggcct gcggcttttc cagctgatag
     6781 cgaaagccgt cgatgcggaa ctgcgtgttt tcgtgcagcg ccgcgatctg cccgtcgtcg
     6841 aagctcagga tggcgcggga accggcggcg gtgctcaccg tgctgcctgc cagcagccgc
     6901 tggccgcgca ccgcgggccg ggtatcggcg ctcgccgcag cgacgcgcac gtcgccctgc
     6961 atctcggaga tgactgccgc cgcgcacgcg ctcgccgccg acagaagacc cgcgattccc
     7021 gttgcgagga ctgcggacgc aaaacgccac cccttgcctg cccccattcc ccctcccttt
     7081 tgcatcgcgg cgccggaacg gccgcgatgt tcagtactgc gccgaactac ggcttgccgg
     7141 gcgtggccgg cttgccgcgc tcgcgcgcgg cctcggaaac cgactggccg aactcgcggc
     7201 cgagttcgcc gcgcgattcg cgcgccttgt tggccacgct gtgtccgaac gccgagcgtt
     7261 cacgtgcccg gtcggacgcg gtggcgggca gctcgatcac cttggtggcg gagtcggacg
     7321 cggcattcgc cggcacgacg tccatcgtca cctcgaggtc acgctgctgc gcccaggcca
     7381 ggccgggcag cgccagcgcg agcgccgcgc cggcagcaat aatcgtcagt cgtttcatcg
     7441 gttgactcct tttcctaccc ctgcggtctt gcttcgatct tcagtcgaaa cctcttgctg
     7501 ctctcgccgt gcgacagcac cgccacgaat tcgccgtttg ccctggcctg cagcaccagc
     7561 ggcaactgca gcaggttggc gccttcgcgc aggtccgtgc tccaggtcag ttcgcgccgg
     7621 tcgccatagc caacgatctg cacgttctcg ggcagccaga ggctcagcgt cgcaccctgc
     7681 agcgccttcg cggaattgaa caccagatgg atgctctcgg gttgctgcac gctgagcgac
     7741 accgtcggcg ccggttccgg gcgaaccgca aaaaggatcg ccgccacgcc caccccgagc
     7801 gccaggctgg ccgcaagcgc catggccggc gccgcgcctg ccggagcggc aacggccttg
     7861 gcaagcgcct gcgccgcaaa gcccgggcgc atcgcgggtg cggcaagctt gcgcatttcc
     7921 gcacgcagcg actccgcgtc cgcatggccc cggcggcagc cctcgcagct ctgcaggtgc
     7981 agccgcaacg gctcctgttc ggcggcgcca agccgcccgt ccagccaatc gtccacccgc
     8041 agcgcaaagt tccggcaatc catggcattc cctcctagcg cttaacacgc tcggaggcgg
     8101 caaaaggttc catccccagg tgggcgcgca gctgctggcg ggcccgatgc aggcgcgact
     8161 tgagggtgcc gaggggcgcc tgcaggatgc cttcgagctc ttcgagcgcg tagccctcga
     8221 tgtcgtgcat cacgacgacc gcgcgctgct cggggttgag ccgccgcagg gcggcaagga
     8281 cgcgctcgcg cagctcgccg cgctcggcaa gctccggcgg gccgtcttcg gccgccggaa
     8341 aggcgtcgag gcgggcttcg tcaccccccg catcgtcagc caggccggag gccggcgagc
     8401 gggcctgccg gcggacaaaa tccacatagg tgcggtagag caccttggcc agccagggcc
     8461 ttagctgctc gactgctgcc agttggcggc tgcgggcgta gagcttgagc aatacgtcct
     8521 gcaccaggtc ctcggcgtcg gccgtgctgc cggtgaagcg ccaggccaga cggtggagga
     8581 actcgacgtg cggccgcaac agggcctcga aactcgcctt ggcgatccgg gtcaggccga
     8641 acggcaagac gcgcaccatg cgcgccacta tacgtccgcc gcggcccgcc tgctttgccc
     8701 tctgcggccc gctttgccaa gtattacgat tgccgcctag ttcttctggg ccagcgtcag
     8761 gccgtcgccg accggaacca gggcaatttc cacacgccgg tcgcgcacca gctggcggtt
     8821 gaaggcgcgg atggcgagcg tgtccaccga cttcttgcgc ggatcggcga cgctgccgcc
     8881 ccacagggtg ttgtccaccg cgatcagccc gccgcggcgc accagccgca ggcaccgctc
     8941 gtagtagttc tggtagttcg ccttgtccgc gtcgatgaag gcgaagtcga acctgccggc
     9001 gcgaccctcc tcgagcagcg catcgagcgt gcgcagcgcg ggcgcgaggc gcagctcgat
     9061 cttcccggcc acgcccgccc gcttccagta gcgccgcgcg atcgcggtcc actcctcgct
     9121 cacatcgcag cagatcaccc gcccggcggg cggcagcgcg agcgccaccg ccagcgccga
     9181 gtagccggtg aaggtgccga tttccaggca gcgccgcgcg cccatcacgc gcaccagcat
     9241 ctgcatcagc gcgccctgct cggcgccgat ctgcatgctc gattgcggca ggcgctgcgt
     9301 gacgctgcgc agctcgcgct gcaccgcggt ctcgcgcgcg ccgtgctcca gcacatagcg
     9361 ctgcaggcgc tcgtccagcg gcagggtgcg cgcggtcact tgaggaacag ccggtaggcc
     9421 gggttgcgcg tctcgtccgc ccagggatag cccaggcgcg cgaggaacct gcggaaatcg
     9481 cccatctcgc gcggcggcac ctcgatgccg accagcacgc tgccgtagtc ggcgccgtgg
     9541 ttgcggtagt ggaacaggct gatgttccag gacggattca tggcgcgcag gaacttcatc
     9601 agcgcgccct ggcgctccgg aaactcgaag cggtagagca attcgtgccg cgccagcgcg
     9661 gccggcccgc cgaccatgtg gcgcacgtgc gccttggcga tctcgttgtc cgacaggtcg
     9721 agggtgcggc agccggcgcg cgccaggcgc cgcgcgagcc gcgcggtctc tgcgcggccc
     9781 gccacgctca cgccgacgaa gatgtgcgcc gcgctcgggc ccgacatgcg gtagttgaac
     9841 tcggtgacgt tgcggttgcc gagcgcctcg aggaagcgcc ggaagctgcc gcggcgctcc
     9901 ggaatcgtca ccgccagcac cgcctcgcgg tgctcgccga gctcggcctg ctcggcgacg
     9961 aagcgcaggc ggtcgaaatt ggtgttcgcg ccggaggcca ccgccaccag ggtcttgccc
    10021 cgggcgccgg tcttcgccac ccaggccttg gcgccggcga tggccagcgc ccctgcgggc
    10081 tccagcacca cgcgcgtgtc ctcgaacacg tccttgatcg ccgcgcacat ctcgtcggtg
    10141 tccacccgca ccatcccgtc gacgagcttc cggcacaggc ggaaggtctc ccggccgacc
    10201 tccttcaccg ccacgccgtc ggcgaacagg ccgacgtgat cgagcttgac gcggcggccc
    10261 gccctcagcg agcgcgtcat cgcgtccgcg tcggcgggct cgacgccgat cacccggatg
    10321 ccggggcgca ggtgcttgag gtagctggcg atgccggaaa tcagcccgcc cccgcccacc
    10381 gcgacgaaca ccgcgtcgat cggcttttcg ctctggcgca ggatctccat gccgatggtc
    10441 ccctgcccgg cgatcacgtc ggggtcgtcg taggggtgca cgaagctcag gccctggcgg
    10501 cgcttgagct gcatcgcgtg ggcgtgggcc tcgtcgtagg agtcgccgtg cagcaccacc
    10561 ttcgcgccgc gcgccgccac cgcgtccacc ttgatcttcg gcgcggtcac cggcatcacg
    10621 atcacggcgc ggcagcccag cttctgcgct gcgagcgcca cgccctgcgc atggttgccg
    10681 gcgctcgccg cgatcacgcc ctggcgcagc cgccggcgcg gcaggttggc catcttgttg
    10741 taggcgccgc gcagcttgaa ggagaacacc ggctgcaggt cctcgcgctt gagcagcagg
    10801 cggttgtgca ggcgccgcga cagcgccggc gcgagctcga gcggcgactc gatcgccacg
    10861 tcgtacaccc gcgcgcgcag gatgcgttcc agatagtctt cgcgtttcac cgcaaaaagc
    10921 ctagcacacc gctccgtgga caccctgctc tcgcccgaag gcggactcgc cggcgtgctc
    10981 gtcgccggtt ttctcgccgc gacgctggtg ccgttctcgt ccgaggcggt gctgttcggc
    11041 tacctgaagc tgcatccggg caatgccggg atcgccgtag cgctcgcgac gctgggcaat
    11101 acggcgggcg ggatgaccag ctacctgctt ggacggttcc tcccgcgcaa ggagttgccg
    11161 catctcgaac tcgtcaggaa atacggtgcg cccgcgctgc tcttcgcctg gctcccgctg
    11221 gtgggcgacg cgttctgcgt ggccgcgggc tggctgcggg tgcactggct cgcggcgctg
    11281 gtctgcatgg ccgcaggcag gctggcgcgc tacctcgcgg tcgccgccct cgcgccgtca
    11341 tgaagccgca acaatcggcc gctagcctgc gcaacacggt gacaaggaga gtgaaaatga
    11401 gaacctggct gatcgccgcg gcgctggccg ccgcggcccc cgcgcatgcg caaatcgtca
    11461 aggtcgacgg ctcgagcacc gtgttccccg tgaccgaggc ggtggccgag gatttccaga
    11521 aagcgggcaa gggcgccgtc aaggtgacgg tgggcatctc cggcaccggc ggcggcttca
    11581 agaagttctg ccgcggcgaa acggacgtgt cgaacgcctc gcgccccatc ctgaggaagg
    11641 agatggagga ctgcaaggcg gccggcatcg agtacatcga gctgccggtg gccttcgacg
    11701 cgctgacggt ggtggtgcac ccgaagaaca gcttcctcaa gcagctcacc gtcgccgaga
    11761 tgaagaagat gtgggagccg ggcgcgcagg gcaaggtgat gcgctggaac caagtgaatg
    11821 ccgcctggcc ggacgcgccg atgaagctct tcggccccgg cgccgactcg ggcaccttcg
    11881 actacttcac cgaggcggtg gtgggcaagt ccaagtcctc gcgcggcgac ttcaccgcct
    11941 cggaagacga caacgtgctg gtgcagggcg tgtcgcgcga tgccaatgcg ctgggctact
    12001 tcggattcgc ctattacgtg gagaacaagg acaagctgaa ggcggtgccg atcgtcaacg
    12061 agaagggcca ggcggtgctg ccttcgctgg aagcggtcga gaagggcagc tacacgccgc
    12121 tggcgcggcc gatcttcatc tacgtcagca ccaaggcgct cgccaagccg gaagtgaagc
    12181 agttcgtgca gtactacatg acccacggcg ccaagctcgc caaggaagtg aagtacgtgc
    12241 cgttgccggc gagcgcctac cagaccgcct gggaccacgt gcaaaagggc aagaaaggca
    12301 ccgtgttcgg cggggtcgcc gaggtcggcg tcaccatcga ggaactgctg cgacgggaag
    12361 ccaggctctg agcaccgcta ccctgcaaca ccacggcgcg cggcttgccg cgcgccgttt
    12421 acgcatcatg cgcgagcgcg ccatcgaggc ggtgctgttc gccgccgcgc tggtgtcggt
    12481 gttcaccacg gtgggcatcg tctacatact ggtgaaggaa tcggtggtgt tcttccagca
    12541 ggtgccgatc gtcgacttcc tcaccgaccg gcagtggacg ccgctgttcg acgacgcgca
    12601 cttcggcatc atggtgctgc tctcgggcac gctcaccagc tcgctggtgg cgctgtccat
    12661 cgccattccg ctcggcacgg tgatcgcgat ctacctgtcg gagttcgcgc ccttcaagct
    12721 gcgcgagatc gccaagccct tcctcgagct gctgggcggc gtgccgacca tcgtctacgg
    12781 ctacttcgcg ctgctgttcg tcacgccgct cctgcagaag atctaccccg ggctgcccgg
    12841 cttcaatctg ctcgccgccg ggctggtgat gggcgtgatg atcatcccct acgtcgcctc
    12901 gatctcggag gacgcgatgc gcgcggtgcc gatggcgctg cgcgaaggct cctatgcgac
    12961 aggcgcgacg cgcctgcaga cggcgctgcg cgtggtgttc ccggcggcct tctccggcgt
    13021 cgccgcagcc tacatcctgg gcatctcgcg cgccgtgggg gagaccatga tcctcgcggt
    13081 ggccgccggc atgcagccca acctcacttt caatccgctg gagcccgccg ccaccatcac
    13141 cgcctacatc gtgcaggtgg ccctgggcga cctgccgcac ggctcggtgg gctaccagac
    13201 catcttcgcg gccggcctga cgctgatgct gatcacgctg gtgttcaaca tcgccgggca
    13261 catgctgcgc aagcgcttcc gggaggccta ttgaaagaca acctctggcg ccgcaagcgc
    13321 tacgaggcga tcttcgccgt cgtcggcctg ctcgcgctga tgatcggcgt gctgaccttc
    13381 gccaccctgt tcgccgaaat ggcgatcaac ggcatgggcc ggctcgactg gagcttcttc
    13441 acctcgttcc cgtcgcgccg gcccggcgag gcgggcatcc tctcggcctg gatcggctcg
    13501 acgctggtga tggtggtgac ggccttcttc gccgtgcccc tcggcgtcgc cgccggcgtg
    13561 tacctcgagg agtacgcgcg caagaactgg gtcaccgatc tcatcgagat caacatcacc
    13621 aacctcgccg gtgtgccctc gatcgtctac ggcctgctgg cgctcggcct cttcgtgtac
    13681 cagttcggcc tgggccagag catcctcgcg gcggggctca ccctcgccct gctgatcctg
    13741 ccggtggtga tcgtcgccac gcgcgaggcg atccgctcga tcccgcaggc gatccgcgag
    13801 ggctcctatg ccctcggcgc cacgcagtgg cagacggtgc gctaccacat cctgccgtac
    13861 tcctcggcgg gcatcctcac cggcgtgatc atcggcatgg cgcgcgccat cggcgagacc
    13921 gcgccgatca tcaccatcgg cgcgctcacc ttcatcgcct tcctgccgcc ttcgccggtg
    13981 caggcggcgc cgccgttcct gtccttcgaa tggctgctgt cgccgttcac ggtgatgccg
    14041 atccagatgt tcaactggac ctcgcgcccc gaggcggcgt tccaggtcaa cgcggcggcg
    14101 gccggattcg tgctggtgat catgaccctg gcgatgaacg gcctggccat ctggctgcgc
    14161 taccgcctgc ggcgcaacat caaatggtag gagcgttaag atgtccgcac cggatacgct
    14221 gatgagcacc gagctgatgc gggaaaggaa ggtggcgctg gcgcccaagg cgcaggtcag
    14281 cggcctggac ttctactacg gcgacttccg cgcgctgaag tcgatcaatc tgaccgtgta
    14341 cgagcagcag gtcacggcgc tgatcggtcc ctcgggctgc ggcaagtcca ccttcctgcg
    14401 ctgcttcaac cgcatgcacg atctctaccc gggcaaccgc tacgcgggca gcatcgtgct
    14461 gcaccccgac aacaccgacc tgctctcgcg cgaggtggat ccgatcgagg tgcgcatgcg
    14521 gatcggcatg gtgttccaga agcccaaccc ctttcccaag tcgatcttcg agaacgtcgc
    14581 ctacggcctg cgcgtgcgcg gcgaggtgtc gcggcgcagg atggcggaga aggtcgagca
    14641 ggcgctggag agcgcggcgc tgctcgacga ggtgaaggac cggctgcacc agcctgcgta
    14701 caacctgtcc ggcggccagc agcagcgcct gtgcatcgcc cgcgccctgg cgacggaccc
    14761 ggaactgctg ctgttcgacg agcccacctc cgccctcgac ccgatcgcca ccgcgtcgat
    14821 cgaggagctg atccacgacc tgaagaagca ggtcacgatc ctgatcgtga cgcacaacat
    14881 gcagcaggcg gcgcgcgtgt cggactacac ggcgtacatg tacctgggcg agctgatcga
    14941 gaccggcgtc accgacgagc tgttcatcaa gccgaggatg aagcagaccg aggactacat
    15001 caccgggcgc ttcggatgac cgagcacgcc tccaagcagt acgacctcga cctcgactcg
    15061 atccgctcgc gcgtgctgca gatgggcggg ctggtggagt cgcagatccg ccgcgccctg
    15121 gaggccttcg gctcgggcga gcgagcgctg atcgagcagg tgatcgccga ggaccaccgc
    15181 gtcaacgcga tggaggtggc cctggacggg gactgcagcc aggtgatcgt caagcgccag
    15241 cccgccgccg gcgacctgcg catgatcttc gccatcacca agacggtcac cgacctggag
    15301 cgcatcggcg acgaggcgcg caagatcgcg cgcatggcga ggagcatcca cgagcgcggc
    15361 gcgcagagcg tgccgctggc agtcgacgtg cgccacgccg ccgagctcgc catcgccatg
    15421 ctgcagcgct cgctcgacgc cttcgcgcgg ctggacgccg acgcggccgc cgaggtgatc
    15481 gccgccgacg ccggcatcga cagcgagttc cgctccatcc tgcgccagct catcacctac
    15541 atgatggaag acccgcgcac catcagcatg gcgctggaga tcgtgtggat cgcgaaggcg
    15601 gtcgagcgca tcggcgacca cgccaagaac atggccgagt acgtgatcta catcgtcaag
    15661 ggcaccgacg tgcggcacac caacccggag cagctggagg catgagcgcc accgtcctcg
    15721 tggtcgagga cgagccgcag atccgggagc tcgtcgtcgt caacctcgag cacgccggcc
    15781 accgcgtgct gcgcgccggc aacgccgagc aggcggagca gtcaatccgc ggcgagctgc
    15841 ccgacgtggt ggtgctcgac tggatgctgc ccgggcagtc cggcctcgac ttcgcgcgcc
    15901 gcctgcgcgc cgacccgcgc acgcgcgagc tgccgatcct gatgctgacg gcgcgcgcca
    15961 tggaagccga caagctcgcc gggctggagg ccggcgccga cgattacctc accaagccgt
    16021 tctcgcccag ggagctcatc gcgcgcatca aggcggtgct gcgccggcgc gcaccgcagt
    16081 acgccggcga cccggtggag atcgcggggc tgcgcctcga cccggcggcg catctggtga
    16141 gcgccggcga gcagcggctc gagctctcgc cggtggagta ccgcctgctg cagttcttca
    16201 tgacgcatcc gggccgcgtg tactcccgcg gccagctgct cgaccaggtc tggggcgacc
    16261 acgtgtacct cgaggagcgc acggtggacg tgcatatccg gcgcctgcgc aaggcgctcg
    16321 agccctcggg gcacgaccgc ctgatcgaca cggtgcgcgg ctcgggttac gcgctgcggc
    16381 cggcgtgagg cgcatcgccc gcggcgcggt cgtcccgttc gctgcgctgg ccttgctggc
    16441 cggacttgcc ggcgccgccg gcggggcagc ctggggctgg ggcgcgctcg ccgcggggct
    16501 cgccgccctg ctcgcgcacc atctgctgaa cctggcgcgg ctcgccgagt ggctgtctgg
    16561 gcaccggccg ggcgaggtgc cggaaggctc gggcagctgg ttcgaggtgt tctcggcgct
    16621 gcaccggcag gagcgctccg cccatcagcg cggcgcgctg ctcgccgagg cgctggcgcg
    16681 cttccgccgc gcggtgcagg cgctgcccga cggcgtggtg atcctcgacg ccgaaagccg
    16741 catcgagtgg tgcaacgcca ccgcggaagc gcagctcggc ctggacgccc gcgccgacct
    16801 cgggcagccg atcgcgaacc tggtgcgcga gccggcgttc gtggcctacc tcggggccgg
    16861 cgaggacgcg gcgccgctgc gcgtcgagcc gcgccccggc cgcgccctgg tgctgcagct
    16921 gattccctac ggcggcaccg agcggctgct gctgtcgcgc gacgtcacgc aggccgagcg
    16981 cctggagacc atgcggcgcg acttcgtcgc caacgtctcg cacgagctgc gcacgccgct
    17041 caccgtgctc tcgggcttcc tcgagacgct gcgcgagttg aagctcgacc cgcagcgcca
    17101 gcgcgactac ctcgacatga tgcgcgagca gtccgcgcgc atgcagggga tcatcgggga
    17161 cctgctcacg ctctccacgc tggaatcggc gccgccgccg gccgccgcgc gcgtgcgcgt
    17221 ggcgcctctc ctcgagcgcc tgcacgccga cgcgctcggc ctgtcgggcg gccggcacga
    17281 gatctcgctg caggggcggc cggcggtgga cctcgccggg tcggaggccg agctcgccag
    17341 cgccttcggc aacctggtga gcaacgcggt gcgctacacg ccgcgcggcg gcaaggtgac
    17401 gctgctgtgg agcgacgatg cgctcggcgc gcagttcgcc gtggcggaca ccggcgtggg
    17461 cgtcgccccg gagcacatcc cgcggctcac cgagcgcttc taccgcgtgg accgcggccg
    17521 ctcgcgcgag agcggcggca cggggctggg cctggcgatc gtcaagcacg cgctggcgcg
    17581 ccaccaggcg acgctgcaca tcgagagcac gcccggcgcc ggcagccgct tcgcggtccg
    17641 cttcccggcg cagcgcaccc tgcccctcac cgcggaggtg gacgctatgc cgcctgcagc
    17701 ttcgccaccg ccaccgccag ccacttcacg ccctgccggc cgaaattgac ctgcacgcgc
    17761 gcgtcgctgc cctggccttc ggcgtccacg atcacgccgc tgccgaactt cgcatggttc
    17821 acgttctggc cgatgcggaa ggcgccgccc gcgccgccgg tgccggccgg cgcgccgccg
    17881 aagcgcgggt cgcgcttggg cgcgcgcgtg aacgagccgg cggtgaagtg cgacaccgcc
    17941 ggcttgggct tctgcgcaaa gcgcggcgtg agccacttga gcaagccctg cggaatttcg
    18001 tcgaggaagc gcgaaggcag gttgtagcgc gtctgcccgt gcaacatgcg cgtctgcgcg
    18061 aaggagaggt acaggcgctt ccgcgcgcgc gtcagcgcca cgtacatcag gcggcgctcc
    18121 tcttccgggc cgtccttctc cagcaggctg ttttcgtggg gaaagaggcc ctcctccagg
    18181 ccgctgacga acaccgcgtt gaattcgagg cccttggccg agtgcatggt catcagctgc
    18241 agcgcgtcct ggccctcgcc cgcctggtgc tcacccgcct ccagcgcggc atgggtcagg
    18301 aacgcggtga gcgggtcggc ctcctcgccc gattcgaccg ccgcatcctg caggctgaag
    18361 gcggcggcgg cgttcaccag ctcgttcagg ttctcgatgc ggtcggcccc ctcgcgctcg
    18421 gtcttgtagt gctcgatgag gcggcttttc gccagcgctt gctcgaccgt ctcggcgagc
    18481 gtgaggccct gcgtttcctc gcgcagcgac tcgatgagcg cgatgaaggg ggcggacttg
    18541 ccgggcgcgg cgagcgccgt ctcgtacagg ctcgtgcccg ccgccttggc cgcgtcctgc
    18601 agcgcctcca cagtgcgcgc gccgatgccg cgcgagggga agttcaccac gcgcaggaag
    18661 gcgttgtcgt cggccggggc ggcgaccagg cgcaggtagg cgagcgcgtg cttcacctcg
    18721 gcgcgctcga agaagcgcag gccgccgtac acgcggtagg gcagcgcggc ggtgaacagg
    18781 gcgtgctcca gcacgcgcga ctgggcgttc gagcggtaca ccaccgcgat ttggtcgagg
    18841 cgcgagccct cgcgctgcag ggccttgatc tcctccacca tccagcgcgc ctcgtccacg
    18901 tccgactgcc cctcgaacac gcgcagcggc tcgccggcgc cggccgcggt ccacaggttc
    18961 ttgcccaggc ggttcttgtt gttggcgatg agcgcgttgg cggcgtcgag gatgttgccc
    19021 tgcgagcggt agttctgctc caggcgcacc accttcgcca ccttgtagtc gcgctcgtag
    19081 tcggccatgt tgccgacgtt ggcgccgcgg aaggcgtcga tcgactggtc gtcgtcgccc
    19141 accgcaaaca ggctcgttag cggcccggcg aggagcttca gccagcggta ctgcagcttg
    19201 ttcgtgtcct ggaactcgtc caccagaatg tggcggaagc gcgcgcggta gtgctcgcgt
    19261 aaaggctcat tcctactcag cagctcgaag cttctcagca gcaactcggc gaagtccacc
    19321 actccctcgc gctggcactg ctcgtcgtag gcggcgtaga actccacgaa gcggcgcgtg
    19381 ttgtcgtcgg tgagcggcac gtcgcgcgcg cgcaggccct cctccttctt ccccataatg
    19441 aagtactgca cgtcgcgcgg cgggaagcgg tcttcgtcga cgttcatcgt gcgcagcagc
    19501 cgcttcactg cggcaagctg gtcctgcgaa tcgaggatct ggaaagtgcg cggcagcccg
    19561 gcttcctggt ggtgcgcgcg cagcatgcgg ttgcacagcc cgtggaaggt gccgatccac
    19621 atcccgcgcg ggttcgccgg cagcatcgtg cccaggcgcg tcagcatctc gcgcgccgcc
    19681 ttgttggtga aagtgaccgc cagcacaccg gagggcgaaa cgctgccttc cttgatcagc
    19741 caggcgatgc gcgtggtgag cacgcgcgtc ttgccgctgc cggcgccggc gaggatcagc
    19801 gcatgccctt cgggcagcgt gacggcggcg agctgttcgg ggttgaggtg ttcgaggtga
    19861 agcgacaagc ctgcatttta ctggggctcg agcttcagtt cctttgcaat tgcggtccag
    19921 cgctcgatgt cggcgcggat cagcgcttcg aattctttcg gcgtgctgcc gacggccagc
    19981 agcgcagcct gcgcgaggcg ctcctttacg tcctgcagct cgagcgcggt tctggcttct
    20041 cgatggat
//
LOCUS       DASRIE010000181         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3576422, whole genome shotgun sequence.
ACCESSION   DASRIE010000181 DASRIE010000000
VERSION     DASRIE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576422"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFB53_07585"
     CDS             <1..208
                     /locus_tag="VFB53_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN86630.1"
                     /translation="LLVEIAELTPPLGFNIFVLQTMTGRDSNYVALASAPFFLMMVLC
                     IALITLFPELATWLPDTLIGVAKK"
     gene            205..888
                     /locus_tag="VFB53_07590"
     CDS             205..888
                     /locus_tag="VFB53_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZN86631.1"
                     /translation="MSRRMAAARDTLVVTVRQRCFWLFAVLLLLLVSVPFLEASPGGR
                     IVLNVASLLTLIAGATAITRRAGATLITLLLAVPTALSLLLAVVYDQPQFRVWSQVFG
                     AGFYFAVTSYLLSYTLRRDVMTMDKLYGAAAAFLLLGVLWGFLYSILLTFYPGALAMN
                     GVPMTGAAAPSTMLYFSFVTLTATGMSDVMPVHPVARMLCSLEMITGVLFIAVLIARL
                     AGSYPPREG"
     gene            888..1523
                     /locus_tag="VFB53_07595"
     CDS             888..1523
                     /locus_tag="VFB53_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN86632.1"
                     /translation="MATRAVVLDVGGVLARDIWEPMFDDLAVRYGADRERLHEAGRLL
                     WETFAYVPETPANTARDMERRYWELFLLICRLPLTVEALIALTDDYIVPVPGMRAIVE
                     ALHARGTPLVICSNNNEIWWERQAAKLELDRFFRPECLILSSRTGAPKDSPGLEMFRA
                     VVAAAGVPAAACFFVDDREPNIVRARAFGLDAVLFRGPDAFADELRKRALL"
     gene            complement(1520..>2118)
                     /locus_tag="VFB53_07600"
     CDS             complement(1520..>2118)
                     /locus_tag="VFB53_07600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN86633.1"
                     /translation="FYVCPGLVDRAKWLWTHETRRRLARLSPAARAELAREWAASADS
                     NEFVAWMKTLDSAARRRVEQRVQEATPQFTPTPEERDDYDLADWKALRRLDPSIITIG
                     SHTLTHPILPGVPADELEAEVRDSRRQLERSLERPVDSFAYPNGDLDAAADACVRRHY
                     RVALRASGGCVNRGVDLHRLPRIAAPRGVLRLARRIYS"
BASE COUNT          273 a          743 c          728 g          374 t
ORIGIN      
        1 gctgctggtc gagatcgccg agctcacccc gccgctcggc ttcaacatct tcgtgctgca
       61 gaccatgacc gggcgcgaca gcaactacgt cgcgctcgcc tcggcgccgt tcttcctgat
      121 gatggtgctg tgcatcgcgc tcatcaccct gttccccgag ctcgccacct ggctgccgga
      181 tacgctcatc ggcgtggcga agaaatgagc cgccgcatgg cggcggcgcg cgacaccctg
      241 gtcgtcaccg tgcggcagcg ctgcttctgg ctgttcgcgg tgctgctgtt gctcctggtc
      301 tcggtgcctt tcctggaggc gagcccgggt ggacgcatcg tgctcaacgt ggccagcctg
      361 ctgacgctga tcgccggcgc cacggcgatt acccggcggg caggtgccac gctgatcacg
      421 ctgctgctcg cagtgccgac ggcgctgtcc ctgctgctcg cggtggtcta cgaccagccc
      481 cagttccggg tctggtcgca ggtcttcggc gcggggttct acttcgccgt gaccagctac
      541 ctcctgtcct acaccctgcg gcgcgacgtg atgaccatgg acaagctcta cggcgcggcc
      601 gcggcgtttc tgctgctcgg cgtcctgtgg ggcttcctgt actccatcct gctcaccttc
      661 taccccggcg cgctcgcgat gaacggcgtg ccgatgaccg gcgccgccgc tccgagcacg
      721 atgctgtact tcagcttcgt caccctcacc gccaccggca tgagcgacgt catgccggtc
      781 catccggtcg cgcgcatgct gtgctcgctg gagatgatca ccggcgtgct gttcatcgcc
      841 gtcctgatcg cgcgcctggc cggcagctac ccgccgcgcg agggctgatg gcgacgcgtg
      901 ccgtcgtcct ggatgtcggc ggcgtgctcg cgcgcgacat atgggagccg atgttcgacg
      961 acctggcggt gcgctacggt gccgatcgcg agcggcttca cgaggcggga cgcctgttat
     1021 gggaaacctt cgcgtatgtg ccggagaccc cggccaatac cgcgcgcgac atggaacggc
     1081 gctactggga gctcttcctc ctcatctgca ggctgccgct cacggtggag gctctgattg
     1141 cgcttaccga cgactacatc gtcccggtac cgggcatgcg ggcaatcgtc gaagcgctgc
     1201 acgcacgcgg cacgccgctc gtcatctgct cgaacaacaa cgagatctgg tgggaacggc
     1261 aggctgcaaa gcttgagctc gaccgtttct tccggccgga atgcctgatc ctctcgtcgc
     1321 ggaccggcgc gcccaaggac agccccgggc tggagatgtt ccgcgccgtg gtcgccgccg
     1381 ccggcgtgcc ggccgccgcc tgcttcttcg tcgacgatcg cgagccgaac atcgttcgcg
     1441 ccagggcgtt cgggctcgac gccgtgctgt tccgcgggcc ggacgccttc gcggacgagc
     1501 tcaggaagcg cgcgctgctc tagctgtaga tgcggcgcgc gagccgcagc acgccgcgcg
     1561 gcgcggcgat gcgcggcagg cgatgcaggt cgacgccgcg gttcacacag ccgccgctcg
     1621 cgcgcagcgc gacgcggtaa tgccggcgca cgcaggcatc ggcggcggca tcgaggtcgc
     1681 cgttcggata ggcgaagctg tcgaccgggc gctcgaggct gcgctcgagc tggcgccggc
     1741 tgtcgcgcac ctcggcctcg agctcgtccg ccggcacgcc gggcagtatc ggatgggtaa
     1801 gcgtgtgcga gccgatggtg atgatggacg ggtcgagacg gcgcagcgct ttccagtcgg
     1861 cgaggtcgta atcgtcgcgc tcctcgggcg tgggcgtgaa ctgcggggtt gcttcctgca
     1921 cgcgctgctc gacgcgcctg cgcgcggcgc tgtcgagcgt cttcatccag gcgacgaact
     1981 cgttgctgtc cgcgctcgcc gcccactcgc gcgccagctc ggcgcgcgcc gcggggctga
     2041 ggcgcgcgag gcggcggcgc gtttcgtgcg tccagagcca tttcgcgcga tccaccagcc
     2101 cggggcagac gtagaacg
//
LOCUS       DASRIE010000182         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_191076, whole genome shotgun sequence.
ACCESSION   DASRIE010000182 DASRIE010000000
VERSION     DASRIE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191076"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFB53_07605"
     CDS             <1..1130
                     /locus_tag="VFB53_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN86634.1"
                     /translation="LVMVVPPALERRGPAIPREAIFVIDTSGSMQGASIAQAREALEL
                     AIRRLAGADRFNVIEFNSHARLLYAEARPATPDNIAGAVRWVRDLRAQGGTEMAKALD
                     LALDGRETAGRIRQIVFLTDGAVGNEDHLLRMIRERLGDSRLFTVGIGSAPNSHFMTK
                     AAQFGAGTFTYIGRIEEVKEKMDALFARLESPVLKGVRIDWGGAEEVEAWPKQVPDLY
                     AGEPVMVLFSAARLGSGGITVSALAGDRPWQERVLLVPAAGENALSVLWARERIGALM
                     DQLRGGAPEQEVRDAVLKLALEHRLVSRYTSLVAVDKTPARGAEAALKSASLATNLPE
                     GWTYEGVFGELPRGATGMRFDFLAGALLLLLAGLLWRRAGA"
     gene            1109..1759
                     /locus_tag="VFB53_07610"
     CDS             1109..1759
                     /locus_tag="VFB53_07610"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HZN86635.1"
                     /translation="MAARRRLTLVAALVLAFAGAACLGQGAWIYAKAQLAQLLIERAW
                     QRALAGESAPKPWPWADTWPVARLDAPGRGISLFVLAGSSGRTLAFGPGHEQGSPLPG
                     TRGNSVIGGHRDTHLGFLEKIEPDDSIWIQRPDGVRIEYRVTQLDVLDKRDTWVTRND
                     GESRLTLITCWPFDALRAGGPERYVVIAVASTPAPRAPGSRAGSAGAPAATASRSR"
     gene            complement(1680..>2193)
                     /locus_tag="VFB53_07615"
     CDS             complement(1680..>2193)
                     /locus_tag="VFB53_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZN86636.1"
                     /translation="PWVRWPLMAWVDFFRAFPPLVLLILLFAGLPFAGLELSGFACVC
                     IAFFLNTGAYYGEILRAGIDSVPAGQTEAARSTGLGRLQTTLFVVLPQAVRNVLPDLL
                     SNTLEVVKLTSLGSVVAMPELLFQARQAQSLTYNPTPIVLAALIYFVMLWPLVRLLSR
                     LENRALAGRG"
BASE COUNT          340 a          769 c          777 g          307 t
ORIGIN      
        1 gcctggtcat ggtcgtgccg ccggcgctcg agcgccgcgg cccggcgatc ccgcgcgagg
       61 ccattttcgt catcgacact tccggctcga tgcagggagc gtcgatcgcg caggcgcgcg
      121 aggcgctcga gctcgcgatc cgcaggctgg cgggcgccga tcgcttcaac gtgatcgagt
      181 tcaacagcca tgcacgcctc ctctacgcgg aggcgcgccc ggccacgccc gacaacatag
      241 ccggcgccgt acgctgggtg cgggacctgc gcgcgcaggg cgggaccgag atggcgaagg
      301 cgctcgacct cgcgctggat ggccgcgaaa cggcggggcg catccggcag atcgtgttcc
      361 tcaccgacgg cgcggtgggc aacgaggacc acctgctgcg catgatccgc gagcgcctgg
      421 gcgactcgcg cctgttcacg gtgggcatcg gctcggcgcc caacagccat ttcatgacca
      481 aggcggcgca gttcggcgcc ggcaccttca cctatatcgg ccgcatcgag gaggtgaagg
      541 aaaagatgga cgccctgttc gccaggctgg aatcgccggt gctcaagggg gtgcgaatcg
      601 actggggcgg cgccgaagag gtcgaagcct ggccaaagca ggtgcccgac ctctacgccg
      661 gcgagccggt gatggtgctg ttctccgccg cgcgcctggg cagcggcggg attaccgtct
      721 cggcgcttgc cggcgatcgg ccctggcagg agcgcgtgct gcttgtcccg gccgcaggcg
      781 agaacgcgct gtcggtgctg tgggcgcggg agcggatcgg cgccctcatg gaccagctgc
      841 gcggcggcgc gccggagcag gaggtccgcg acgcggtgct gaaactcgcg ctcgagcatc
      901 ggctggtgtc gcgctacacc agcctggtcg ccgtcgacaa gacgccggcc cgcggcgccg
      961 aggccgcgct caagtccgcg tcgctggcga ccaacctgcc tgaaggctgg acctacgaag
     1021 gcgtcttcgg cgagctgccg cgcggcgcca ccggcatgcg cttcgatttc ctggccggcg
     1081 ccctgctcct cctgctggcc ggtctgctgt ggcggcgcgc cggcgcctga cgcttgtcgc
     1141 agcgctggtc ctggcgtttg cgggcgcggc ctgtcttgga cagggcgcgt ggatctacgc
     1201 caaggcgcag ctcgcccagc tcctcatcga gcgcgcctgg cagcgcgccc tcgccgggga
     1261 gtccgcgccc aagccctggc cgtgggccga cacctggccc gtcgcgcgcc tcgacgcccc
     1321 cggccgggga atctcgctct tcgtcctcgc cggatcgagc ggccgcaccc tcgccttcgg
     1381 ccccggccac gagcaaggct cgccgctccc cggcacgcgc ggcaacagcg tcatcggcgg
     1441 gcaccgcgat actcatcttg gttttcttga aaagatagaa ccggacgact ccatctggat
     1501 ccagcgcccc gacggcgtgc gcatcgaata ccgcgtaact caactcgacg tgctggacaa
     1561 gcgcgatacc tgggtgacgc gcaacgacgg cgaaagccgc ctgaccctca tcacctgctg
     1621 gccattcgac gcactgcgcg ccggcggccc cgagcggtac gtcgtgatcg ccgttgcctc
     1681 tacccccgcc ccgcgagcgc ccggttctcg agccggctca gcaggcgcac cagcggccac
     1741 agcatcacga agtagatgag ggccgcgagg acgatgggcg tggggttgta ggtcaggctc
     1801 tgcgcctgcc gcgcctggaa gagcagctcc ggcatggcga cgacgcttcc cagtgaagtc
     1861 agcttgacca cctcgagcgt gttggagagg agatcgggca gcacgttgcg cacggcctgc
     1921 ggcaggacca cgaacagcgt ggtctgcagg cgacccagac cggtgctgcg cgcggcttcg
     1981 gtctggcccg ccggaacgga gtcgatgccg gcgcgcagga tctcgccgta gtaggcgccg
     2041 gtgttgagga agaaggcgat gcagacgcag gcgaaaccgc tcagctccag cccggcgaac
     2101 ggcaggccgg cgaaaagcag gatcagcagc accagcggcg gaaacgcgcg gaagaaatcg
     2161 acccaggcca tcagaggcca gcgcacccag ggc
//
LOCUS       DASRIE010000183         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3050715, whole genome shotgun sequence.
ACCESSION   DASRIE010000183 DASRIE010000000
VERSION     DASRIE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050715"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1028
                     /locus_tag="VFB53_07620"
     CDS             21..1028
                     /locus_tag="VFB53_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN86637.1"
                     /translation="MALAIFGLAIIGAATAQEKVVNVYSGRHYQTDEALYSGFTKATG
                     IKVNRIEAGEDAIIERIRNEGARSPADVMITVDAGRLWRAEQLGLFQPVKSAVLDERV
                     PASLREPGGQWFGFSSRARVIAYNKAKVQPGEIKTYEELAGPKWKGRVCMRSSANVYN
                     LSLMGALIDHLGEARAEAWAKNVRANLAQEPKGGDTDQLKAVAAGLCDVTISNQYYYA
                     RLVRSDKAAEKQAAERLGIVLPNQESWGTHVNISGAGVLKNAPHRDHAVKFLEYLASD
                     DAQRYFADGNNEWPVVRSVKVDNPVLNALGDFKRDALNVAVLGKNQPGSQKIYDRVAW
                     K"
     gene            complement(1052..2653)
                     /locus_tag="VFB53_07625"
     CDS             complement(1052..2653)
                     /locus_tag="VFB53_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZN86638.1"
                     /translation="MLGATAVTIAALLALPVVYVVTSFFGGAGDTWAHLVATVLPRYV
                     LNTALLLALVACGVISIGVVSAWLVTAYRFPGRDLLEWALLLPLAMPAYVMAYAYTDW
                     LQFTGAVQTALRELTGWQARDYWFPEVRSLPGAAVMLSFALYPYVYLVARTAFHDLSR
                     SAIEAGRLAGYGALGAFLRVAVPLARPAIAAGAALALMETLADFGTVSYFGIEVFTTG
                     IFKAWLSMGDSVAAAQLSACLLAFVVMLLALERVNRGRAAYHGPAPRRARPHPLRGGV
                     AVLALAACAAPVAFGFALPALLLGWLAWGETLSAARLGALMANSFSIAALTALIAVVA
                     ALVLAYAGRLTRSPLVTLGNRLAGLGYAVPGAVIAVGVLVPLGRLDNALAGWLEGAFG
                     MKPGLLLTGTIAALVYAYLVRLLAVALQTTNAGLAKITPSMEDAARSLGATPAAALAR
                     VHVPLLAPSLATAALLVFVDVLKELPATFALRPFNFDTLAVAAYNLAKDERLAEAAAP
                     SLAIVAAGLLPVIYVSKRYFQGVRG"
     gene            2722..3774
                     /locus_tag="VFB53_07630"
     CDS             2722..3774
                     /locus_tag="VFB53_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86639.1"
                     /translation="MLLEVENLRHAYDDHQVIAGLSFTLARGAIGCLLGPSGCGKTTV
                     LRCIAGFEALQGGEIKLAGRIVSGAGVMLPPERRSIGMVFQDYALFPHLPVAANIAFG
                     LHAAPATERAARVRELAELVGLSAVLDKYPHEISGGQQQRVALARALAPRPELLLLDE
                     PFSNLDIDLRERLSHEVRDIIKASGATAVLVTHDQQEAFATADEIGVLHEGRIQQWAS
                     PYNLYHRPANRFVADFVGQGVFLPAKAVDAGRLEIELGVLQGQIGMEKRQGTHNLEVL
                     LRPDDVVHDDDAPARAEVVHKAFRGAEILYTLRLPSGRKVLALVPSHHNHALGEKIGI
                     RLDVDHVVAFQPETAT"
     gene            complement(3771..>4363)
                     /locus_tag="VFB53_07635"
     CDS             complement(3771..>4363)
                     /locus_tag="VFB53_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZN86640.1"
                     /translation="TARRAVSIPPLLIARLMAPEAPPAPAPVVPEEKSAAAPPAPPTE
                     KLPKPAPAISVEKPAPRVEAPAPPAPVAAPPVAAVEAQPASPPAAAEAPPAQAAESLS
                     RDQYRVQLIDEARRHKRYPALARENNWQGNVLVGVVVGPSGRASVTLKGSSGHEVLDR
                     QALDMFRQAARAVRVPAPLRGKEFALEVRAVYGLED"
BASE COUNT          768 a         1511 c         1509 g          575 t
ORIGIN      
        1 ctgtttttgc agcgcaaagg attgcgctgg ctatcttcgg gctggcgatt atcggggccg
       61 ccacggccca ggaaaaggta gtgaacgttt actccgggcg gcattaccag accgacgagg
      121 ccctctactc ggggttcacg aaggcgaccg gcatcaaagt caaccgcatc gaggccggcg
      181 aggacgccat catcgagcga attcgcaacg aaggtgcgcg cagcccggcc gacgtgatga
      241 tcaccgtcga cgccggacgc ctgtggcgcg ccgagcagct cggcctgttc cagccggtca
      301 agtccgcggt gctcgacgag cgcgttccgg cgagcctgcg cgagccgggc ggccagtggt
      361 tcggcttctc gagccgggcg cgcgtgattg cctacaacaa ggcaaaggtg cagccgggcg
      421 agatcaagac ctacgaagag ctcgccggcc ccaagtggaa gggccgcgtc tgcatgcgct
      481 cctcggccaa cgtctacaac ctgtcgctga tgggcgcgct gatcgaccat ctgggcgaag
      541 cccgagccga agcgtgggcc aagaacgtgc gcgccaacct cgcgcaggaa ccgaagggcg
      601 gcgataccga ccagctcaag gcggtggccg cgggcctgtg cgacgtcacg atctcgaacc
      661 agtactacta cgcgcgcctg gtgcgctcgg acaaggccgc cgagaaacag gccgccgagc
      721 gtctcggcat cgtgctgccc aaccaggaga gctggggaac gcatgtcaac atctccggcg
      781 ccggcgtgct caagaacgcg ccgcaccgcg accacgcggt caagttcctc gagtacctgg
      841 cgagcgacga tgcacagcgc tacttcgccg acggcaacaa cgagtggccg gtggtgcgga
      901 gcgtgaaggt cgacaacccg gtgctgaatg ccctgggcga tttcaagcgc gacgccctga
      961 acgtagccgt gctcggcaag aaccagcccg gctcgcagaa aatctacgac cgcgtagcct
     1021 ggaagtagag gaagcggggg tgagaagcac cttacccccg caccccctga aaatagcgct
     1081 tcgagacgta gatcaccggt agcagccccg ccgcgacgat cgcgagcgac ggggctgcgg
     1141 cttcggcgag gcgctcgtct ttcgccaggt tgtacgccgc caccgccagc gtgtcgaagt
     1201 tgaacggccg cagcgcaaag gtggcgggca gttccttcag cacatcgacg aataccagca
     1261 gcgcggcggt cgccaggctg ggcgcgagca gcggcacgtg cacccgggcg agcgccgccg
     1321 ccggcgtcgc gccgagcgag cgcgccgcgt cctccatgct cggcgtgatc ttcgcgagcc
     1381 cggcgttggt cgtctgcagc gccaccgcga gcagccgcac gagataggcg tagacgagcg
     1441 ccgcgatcgt gccggtgagc agcaggccgg gcttcatccc gaaggctccc tcgagccagc
     1501 cggcgagcgc attgtccagc cggccgagcg gcaccagcac gccgaccgcg atgaccgcgc
     1561 cgggcaccgc gtagccgagg cccgcgaggc ggttgcccag cgtcaccaga gggctgcgcg
     1621 taagccgacc cgcgtaggcc aggacgaggg ccgcgacgac ggcgatcaac gcggttagcg
     1681 ccgcgatgct gaagctgttc gccatcaacg cgcccagccg cgccgccgag agcgtctcgc
     1741 cccaggccag ccagccgagg aggagcgccg gcagcgcgaa accgaacgcc accggcgctg
     1801 cgcacgccgc tagggcgagg acggctacgc cgccacgcag cggatgcggg cgcgcacgcc
     1861 gcggcgccgg accatggtag gcggcgcggc cgcggttcac gcgctcgagc gccagcagca
     1921 tgacgacgaa ggcgagcagg caggcggaga gctgcgccgc cgccacgctg tcgcccatcg
     1981 acagccaggc cttgaagatt ccggtggtga atacctcgat gccgaaatac gacacggtgc
     2041 cgaaatcggc gagcgtctcc atcagcgcga gggcggcgcc ggcggcgatc gcgggacgcg
     2101 ccaagggcac cgcgacacgc aggaatgcac cgagcgcgcc gtagcccgcc agccggcccg
     2161 cctcgattgc gctgcgcgaa aggtcgtgga acgcggtgcg cgcgaccagg tatacatagg
     2221 gatacagcgc gaacgaaagc atcaccgcgg cgccgggaag cgagcgcact tcggggaacc
     2281 agtaatcgcg cgcctgccag cccgtcagct cgcgcagcgc ggtctgcacc gccccggtga
     2341 actgcagcca gtcggtatag gcgtacgcca tgacgtaggc cggcatcgcc agcggcagga
     2401 gcagcgccca ctccaggaga tcgcgccccg ggaagcgata ggcggtcacc agccatgcgc
     2461 taaccacgcc gatcgagatc acgccgcacg cgaccagcgc gagcagcagc gcggtgttca
     2521 gtacgtagcg tggcagcacg gtggcgacca ggtgtgccca cgtgtcgccg gctccgccga
     2581 aaaagctcgt gacgacatag acgaccggca gcgcgagcag cgcagcgatg gtgacggccg
     2641 tggctcccag cacgcctaag ttcttgtaat tcaatgaact aggattgtaa caagaatgag
     2701 aattattact ataatcgcat catgctgctc gaagtcgaga acctgcgcca cgcctacgac
     2761 gatcaccagg tcatcgcggg cctgagcttt acgctcgcgc gcggcgccat cggctgccta
     2821 ctcggaccga gcggctgcgg caagacgacc gtgttgcgct gcatagcggg gttcgaggcg
     2881 ctgcaaggcg gcgagatcaa gctcgccggc cggatcgtgt ccggcgccgg cgtcatgctg
     2941 ccccccgaga ggcgaagcat cggcatggtg ttccaggatt acgcgctgtt cccgcatctt
     3001 ccggtggcgg cgaacatcgc cttcggcctg cacgccgccc cggcgacgga gcgcgcggcg
     3061 cgggtgcgcg agctcgccga actggtcggc ctgtcggccg tgctcgacaa gtatccgcac
     3121 gagatttccg gcggccagca gcagcgcgtc gcgctcgcgc gcgcgctcgc gccgcgcccg
     3181 gagctcctgc tgctcgacga gccgttctcc aacctcgaca tcgacctgcg cgagcggctg
     3241 tcgcacgagg tgcgcgacat catcaaggcg agcggcgcca ctgcggtcct ggtgacccac
     3301 gatcagcagg aggcgttcgc cacggccgac gagatcggcg tcctgcacga ggggcgcatc
     3361 cagcagtggg ccagccccta caacctctac caccggccgg ccaaccgatt cgttgccgac
     3421 ttcgtcggtc agggcgtgtt cctgccggcg aaggcggtcg atgccggccg gctggagatc
     3481 gagctcggcg tgctgcaggg ccagataggc atggaaaagc ggcagggcac gcacaacctc
     3541 gaggtcctgc tgcggcccga cgacgtagtc cacgacgacg acgctccggc gcgcgccgaa
     3601 gtcgtgcaca aggcgttccg cggcgcggag atcctctaca cgctgcgcct gccgagcggg
     3661 cgcaaggtcc tggcgctggt gccgagccat cacaaccacg cactcggcga gaagatcggc
     3721 atccgcctcg acgtcgacca cgtcgtggcg tttcagccgg aaacagcgac ctagtcctcc
     3781 aggccgtaca ccgcccgcac ctccagggcg aactccttgc cgcgcaacgg cgcgggcacc
     3841 cgcactgcgc gcgccgcctg cctgaacatg tcgagtgcct gccggtcgag gacctcgtga
     3901 cccgagcttc ccttcagcgt caccgaagcg cggccgctcg ggccgacgac cacgccgacc
     3961 agcacgttgc cctgccagtt gttctcgcgc gccagcgccg gatagcgctt gtggcggcgc
     4021 gcctcgtcga tcagctgcac gcggtactgg tcgcgcgaca gcgactcggc cgcctgcgcg
     4081 ggcggcgcct cagccgcggc gggaggagaa gcgggttgag cctcgaccgc tgcgaccggc
     4141 ggcgccgcaa ccggagccgg cggtgcggga gcctcgaccc gtggtgccgg cttttcgacg
     4201 gatatcgccg gcgctggctt cggcagcttc tcggtcggcg gcgccggtgg tgcggccgca
     4261 gatttttctt ccggcaccac gggcgccggc gcgggcggag cttccggcgc catcaggcgt
     4321 gcgatgagca gcggcggaat gctcaccgcg cggcgcgcgg tat
//
LOCUS       DASRIE010000184        10440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1237221, whole genome shotgun sequence.
ACCESSION   DASRIE010000184 DASRIE010000000
VERSION     DASRIE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237221"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..959
                     /locus_tag="VFB53_07640"
     CDS             225..959
                     /locus_tag="VFB53_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86641.1"
                     /translation="MRQADAGARLRAALQELETLHQELADFSYSVSHDLRAPLRHIDG
                     FARLLEAELGAPGGKAGRYLATIGAAARRMDALLEDLLQFSRAAREPLAPQRVEPAAL
                     VQALIREAAGTIGERRVEWVVDELPAVTADPALLRTVLRNLLSNALKFTRAREPARIE
                     IRARRLDGGAVELQVRDNGAGFDMQFAEKLFGVFQRLHDDEEFEGRGIGLATARRILH
                     RHGQRIRGEGAPQAGAAFTFTMPAAD"
     gene            981..2387
                     /locus_tag="VFB53_07645"
     CDS             981..2387
                     /locus_tag="VFB53_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN86642.1"
                     /translation="MRVLLLEDDPADAELALAALAAAGFACEAEVAGGRADFERLHAA
                     GGWDLVLADYSLPGYTGLDALARVRAADPLLPFILVSGALGEERAVDALRAGANDFVL
                     KGNLRRLAPAVLRALDEARERREHRAVQQALELSERRYRAVVEDQSELIIRMLPDSTV
                     TFVNRAYCRYYGLQPDQLLGRAVLHSLSPENRAALQRSLAGLSAQSPTYERDALVRDA
                     AGEERWTHWVARGIFDARGTLAEVQSVGRDVTELRRLSRRLLEVQETERRNLARDLHD
                     DIGQALTALKLNLEAVARARDGGALQARLDEALDTTRHALARVRALSLNLRPLQLDDL
                     GLVAALRSHLDRQAALARIAPHLELGEPPAALGLEIESACFRVAQEAINNVVRHAAAG
                     NLWLGLSFGDGRMALTVRDDGRGFDVRAAQRRAAAGESLGVVSMQERVALAGGSFDIR
                     STPGEGTVVSASFPLEAP"
     gene            2387..3037
                     /locus_tag="VFB53_07650"
     CDS             2387..3037
                     /locus_tag="VFB53_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN86643.1"
                     /translation="MAAQRILLVDDHALVRAGMKSLLHEIPGVEVVAEAADGAEALRL
                     AAELRPDVVLLDIAMKGMNGLDAAARLREQLPATKVIVLSMHTSEEYVLLALRAGAAG
                     YLIKDSATSELELALKAVARGETYLSPAISRQVVDGYVQRVGAAATPDPLTPRQREVL
                     KRIAEGRSTKEIAFDLKLSVKTVETHRAQIMERLGIRDVAGLVRYAMRAGLVPPET"
     gene            3135..3464
                     /locus_tag="VFB53_07655"
     CDS             3135..3464
                     /locus_tag="VFB53_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86644.1"
                     /translation="MTQSAAPTRILVLDANPSFVAAAARFIERLPGCARVAADVALVD
                     LGLAGLTLAQRLKAANPALGVIALALFPSPELLAEAQRAGIDAVIAKESFADELPGAL
                     ARLRGAR"
     gene            3461..5614
                     /locus_tag="VFB53_07660"
     CDS             3461..5614
                     /locus_tag="VFB53_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN86645.1"
                     /translation="MSRAFAPPARDAAAEAAAAHRRIEQALRDSEARFRSLTELSSDW
                     YWEQDAELRFVHTGGVASARGGITAAQHVGLRRWELPGTSTLSGSWAEHQAVLAARAP
                     FRDLILQRAGNDGTLRYVSVSGQPIFDAAGRFTGYRGVAKDVTASHQAVMALRESERR
                     LSALIASLPGAAYRCRNDANWSSEFFSEGVRALTGYGPEEFTAGKVSFGDLVHVEDRT
                     RIWEAAQVALRERQPYTMEYRIRSADGQQKWIWERGAGVYGEGGEVLALEGFISDITE
                     RKLAEQRLAHLVQFDPLTGLPNRTLLHDRLSQTVAQAQRQGRQAAVLFVDLDRFKLIN
                     DTLGHHVGDLLIAEVARRLLRCVRAGDTVGRISGDEFGVVLADLAHADDAALVSQKLL
                     EALAAPYQLAGSEAYTSASIGIAVCPGDGTHAEDLLRNADMAMYRAKESTRNAYCFFT
                     PEMNQRASARMQLNNDLRHAIERREFVLHYQPKVDLVSGRMQGLEALLRWNHPERGLV
                     SPAEFIPALEDSGLILPVGEWVLEEACAQLGRWLAAGLVPVPVAINLSAKQFRRRDLD
                     DVIARALAAAGVPSALLELEITESSLMDDPEQAVRLLDSLRRQGVRTSVDDFGTGYSS
                     LSYLTRLPLSALKIDRSFVRDAMTNPEAASIVRAVIDMAHNLRFTVVAEGVETEQQVA
                     FLRQHACDQAQGYLYGRPGTADQVRGRLAPALPRA"
     gene            5611..>10440
                     /locus_tag="VFB53_07665"
     CDS             5611..>10440
                     /locus_tag="VFB53_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN86646.1"
                     /translation="MSNVVPLKPGAAGAVRRILMVEDDANDAELLQACLAKACPEGSV
                     ILHARTLAEGLALQRAHEVQLTLLDLDLPDSSGFGTLERMRAAASGPVVVVSGNSHPA
                     LVDEALARRAYDVIPKDELDAATLRRVLRLATLHHEAGRALRATEGRLRALVENSAEA
                     LVLFDAAGRIEYASASLRRALGYDAAEVIGRNGLMFVQEEDRRAVQEAFPRLVAAEDA
                     SVTMRVRFVHKDGSARLLEATLVNRLADAEVVAIVCSFRDLTREEEQRARFDAAFDNA
                     AVGMAHVDLDGRILLANRRLGEMLGYAREALAGRTMFELSHPEDADATSEERAQLHAG
                     AIPHFAKRKRYLRSDGGVLWAQITVAAERDAQGLVRYETAAFEDVGEQVRAEERLRAS
                     EARLRAIIGAEPECVKVLDDAGLLLDMNPAGLAMIEADSLATVAGQCVYNLVTPASRG
                     AFRALTERACRGERGILEFEVVGLKGTRRWMETHAVPLRDETGRALMLGITRDITERK
                     AAELALRESEARFRSLTELSSDWYWEQDAEYRFTRFDGGAGDAAWGGDQSKSLGLQRW
                     EIPGLSPLNCTWEEHRALLDARKPFRGVEYLRLTDQGERRYVTTSGEPVFGADGAFLG
                     YRGTAADITERKTAELAVRESESRFRSLTELSSDYYWETDAEHRLSLRGSGGTAVARS
                     TFGPGGGVGQRRWELPFKSPDMDGWMAHKAVLDQHEPFAGFEFSRPTPEGGERFFSIT
                     GGPVYDAEGRFAGYRGVGSDITARKLAERRLRLEHEVARILAAAPDARAGLQGAIRVV
                     CETEVWDCGRYFEVDAGAGLLRHSEAWGVDDPEVRRFLEDSRGMTLARGDGLAGRVWD
                     SGEPAWVADIRKDARVSPQGLAIMGEMRGAFVFPVTLHSGTVGVLAISGREVREPDAA
                     LLRTMATVGNQLGQFLSRQRAEEATRRSEERFRSLTKMSSDFYWETDAAHRFTQRGTG
                     AKNTAVPAFRDGTMFGKTRWELPFVKPDMAGWMAHKAVLDAHLPFRDFEFSRLGADGA
                     EHAIVISGEPVFDGAGNFTGYRGVGSDITERKLAERRVHLEHQVAQLLAAAPEARSAL
                     RGVIRTICETEGWDCGRYFEVDAGANLLRYSEGWGVDDPEVQRFLRETRVLTHRPGEG
                     VRGMVWQSGEPIWVADVRNDPRVTARAQATLGEIRGAFVAPVSAGATVVGVLSISSRV
                     MRQPDERLLKAMGVIGSQLGQFLQKTRGEQALRDSEERFRNLTELSSDWYWEQDAEFR
                     FTKFGMDASSPVYGTQSEPLGKTRWELPLTPLSSTFEQHRETLRAHRTFRDFEYSYAG
                     ADGKTRYVAASGYPVHDAEGRFLGYRGIARDITPRMQSEARMRVHAERQAAIAGFGQF
                     AIGRRSTEELYTEAARALRCEGVDAVCLLEMHTERREFLVRAAQGEGPHASVGLTGPV
                     ASDSVWPEILRENASRIAGREYLAGRPIDRPWRAWLHGMGSAVYTPVRDDEKPIAMLC
                     MYAVREQAFGADDVRFAESMSHVLSTALQRQKAEERLARMAQFDALTGLPNRTLLQDR
                     LGQTIVQSRRRQLHAGVLFVDLDRFKLVNDTLGHHQGDALIRQVGQRLLACVRPGDTV
                     "
BASE COUNT         1437 a         3781 c         3888 g         1334 t
ORIGIN      
        1 gctcagctgg gagagcgccg cgttcgcaat gcggaggtcg agggttcgat ccccttccac
       61 tccaccagat gacccaaggg ccggcaccat gtccacgcgc cgggcccttt ctctttttgt
      121 cccgggcgcc aacgagtgtt aaatagttag cgcattgcgg ctgtccgccc ctcgcgggcg
      181 ggggagaatg aacgcaccat ggcggcgctc gagtggtgac gcagatgcgg caggccgatg
      241 ccggggcgcg cctgcgcgcc gcgctgcagg agctcgagac gctgcaccag gagctggcgg
      301 acttcagcta ttccgtgtcg cacgacctgc gcgcgccgct gcgccacatc gacggcttcg
      361 cgcggctgct ggaggcggag ctcggcgcgc cgggcggcaa ggcggggcgc tatctcgcca
      421 ccatcggcgc cgccgcaagg cgcatggacg ccctgctcga agacctgctg cagttctcgc
      481 gcgccgcgcg ggagccgctc gcgccgcagc gcgtcgagcc ggcggcgctc gtgcaggcgc
      541 tgatccgcga agcggcgggc acgatcggcg agcgccgggt ggagtgggtg gtggacgagc
      601 tgccggcggt gaccgccgac ccggcgctgc tgcgcaccgt gctgcggaac ctgctgtcca
      661 acgcgctcaa gttcacgcgc gcgcgggagc cggcgcgcat cgagatccgc gcacggcgcc
      721 tggacggggg cgcggtcgag ctgcaggtgc gcgacaacgg cgcgggcttc gacatgcagt
      781 tcgcggaaaa gctgttcggc gtgttccagc gcctgcacga cgacgaggaa ttcgagggcc
      841 gcggcatcgg cctcgccacc gcgaggcgca tcctgcaccg gcacggccag cgcatccggg
      901 gcgaaggcgc gccgcaggcg ggagcggcgt tcacgtttac catgccggca gcggactaaa
      961 ggaccgggga ggccgcccgc atgcgcgtgc tgctgctgga ggacgacccc gccgacgccg
     1021 agctcgccct ggctgcgctc gccgccgccg ggttcgcctg cgaggccgag gtggcgggcg
     1081 ggcgcgccga cttcgagcgg ctgcacgcgg cgggcggctg ggacctggtg ctcgccgact
     1141 acagcctgcc cggctacacc ggcctggacg cgctcgcgcg ggtgcgcgcc gccgacccgc
     1201 tgctgccgtt catcctggtg tcgggggcgc tcggcgagga gcgcgcggtg gatgcgctgc
     1261 gcgccggggc gaacgacttc gtgctgaagg gcaacctgag gcgcctcgcg ccggcggtgc
     1321 tgcgcgccct ggacgaggcg cgcgagcgcc gcgagcaccg cgccgtgcag caggcgctcg
     1381 agctctccga gcggcgctac cgcgccgtgg tcgaggacca gtccgagctg atcatccgca
     1441 tgctgccgga ttccaccgtg accttcgtca accgcgccta ctgccgctac tacggcctgc
     1501 agcccgacca gctgctcggc cgcgcggtgc tgcacagcct gtcgcccgaa aaccgtgccg
     1561 ccctgcagcg cagcctggcg ggcttgagcg cgcagtcgcc cacctacgag cgcgacgcgc
     1621 tggtgcgcga cgcggcgggg gaggagcgct ggacgcactg ggtggcgcgc ggcatcttcg
     1681 acgcgcgcgg cacgctcgcc gaggtgcagt cggtgggccg cgacgtgacc gagctgcgcc
     1741 gcctgtcgcg gcgcctgctc gaagtgcagg agaccgagcg gcgcaacctg gcgcgcgacc
     1801 tgcacgacga catcggccag gcgctcaccg cgctcaagct caacctggag gcggtggcgc
     1861 gcgcgcgcga cggcggcgcg ctgcaggcgc gcctcgacga ggcgctggac accacgcgcc
     1921 acgccctcgc gcgcgtgcgc gcgctgtcgc tcaatctgcg cccgctgcag ctcgacgacc
     1981 tgggcctcgt cgccgcgctg cgctcgcacc tcgaccggca ggcggcgctg gcgcgcatcg
     2041 cgccgcacct ggagctcggc gagccgcccg ccgcgctcgg cctggagatc gagagcgcct
     2101 gtttccgcgt ggcgcaggag gcgatcaaca acgtggtgcg ccacgccgcc gccggcaacc
     2161 tctggcttgg cctgagcttc ggcgacgggc gcatggcgct cacggtgcgc gacgacggca
     2221 ggggcttcga cgtgcgcgcg gcgcagcgcc gcgccgccgc cggcgagagc ctcggcgtgg
     2281 tgagcatgca ggagcgcgtg gcgctcgccg gcggcagctt cgacatccgc tcgacgccgg
     2341 gcgagggcac ggtggtgagc gcctcattcc cgctcgaggc gccctgatgg cggcccagcg
     2401 catcctgctg gtggacgacc acgcgctggt gcgcgccggc atgaagtcgc tgctgcacga
     2461 gatcccgggg gtggaagtgg tggccgaggc cgccgacggc gccgaggcgc tgcgcctggc
     2521 cgccgagctg cgcccggacg tggtgctgct ggacatcgcc atgaagggca tgaacgggct
     2581 ggacgccgcc gcgcggctgc gcgagcagct gcccgcgacc aaggtgatcg tgctgtcgat
     2641 gcacaccagc gaggagtacg tgctgctcgc gctgcgcgcg ggcgcggccg gctacctgat
     2701 caaggactcg gccacctcgg agctggaact cgcgctgaag gccgtggcgc gcggcgagac
     2761 ctacctgagc ccggcgatct cgcgccaggt ggtcgacggc tacgtgcagc gcgtgggcgc
     2821 ggccgccact cccgacccgc tcaccccgcg ccagcgcgag gtcctcaagc gcatcgccga
     2881 ggggcgcagc accaaggaaa tcgccttcga cctcaagctc tcggtgaaga cggtggagac
     2941 ccaccgcgcc cagatcatgg agcgcctggg catccgcgac gtcgccggcc tggtgcgcta
     3001 cgccatgcgc gcgggcctgg tgccgccgga aacctgagat ttttcagtgt tttgcagttc
     3061 ataggggata tccctagcag ctttagggaa gcaccctatt ctgcgcgccc ggaggcgcgg
     3121 atactgtgcc tgccatgacg cagtccgccg cccccacccg catcctggtg ctcgacgcca
     3181 atccgagctt cgtcgccgcc gcggcgcgct tcatcgagcg cctgcccggc tgcgcgcgcg
     3241 tcgccgccga cgtcgcgctg gtggacctgg ggctggcggg gctcacgctc gcgcagcggc
     3301 tcaaggcggc caacccggcg ctgggcgtga tcgcgctggc cctgttcccg agccccgagc
     3361 tcctcgccga ggcgcagcgc gcgggcatcg acgcggtgat cgccaaggag tccttcgccg
     3421 acgaactgcc cggcgcgctg gcgcgcctgc ggggggcgcg ttgagccgcg ccttcgcccc
     3481 gccggcgcga gacgctgcgg ccgaggccgc cgccgcgcac cggcgcatcg agcaggcgct
     3541 gcgcgacagc gaagcgcgct tccgcagcct gaccgagctc tcctcggact ggtactggga
     3601 gcaggacgcg gagctgcgct tcgtgcacac cggcggcgtg gcgagcgcgc gcggcggcat
     3661 cacggccgcg cagcacgtcg gcctgcgccg ctgggagctg ccgggcacct ccacgctgag
     3721 cggcagctgg gccgagcacc aggcggtgct cgccgcgcgc gcgccgttcc gcgacctgat
     3781 cctgcagcgc gccggcaacg acggcacgct gcgctacgtc agcgtctccg gccagccgat
     3841 cttcgacgcc gccggccgct tcaccggcta ccgcggcgtg gcgaaggacg tcaccgccag
     3901 ccaccaggcg gtgatggcgc tgcgcgagag cgagcggcgc ctgtcggcgc tgatcgccag
     3961 cctgccgggg gccgcctacc gctgccgcaa cgacgccaac tggagctcgg aattcttcag
     4021 cgaaggggtg cgcgcgctca ccggctacgg gccggaggag ttcaccgcgg gcaaggtgtc
     4081 cttcggcgac ctggtgcacg tggaggaccg cacgcgcatc tgggaggcgg cgcaggtggc
     4141 gctgcgcgag cgccagccct acaccatgga ataccgcatc cgcagcgccg acggccagca
     4201 gaagtggatc tgggagcgcg gcgccggcgt gtacggggag ggcggcgagg tgctcgcgct
     4261 ggagggtttc atctccgaca tcaccgagag gaagctcgcc gagcagcgcc tggcgcacct
     4321 ggtgcagttc gacccgctca ccgggctgcc caaccgcacg ctgctgcacg accgcctgtc
     4381 gcagaccgtg gcgcaggcgc agcgccaggg gcgccaggcc gcggtgctgt tcgtcgacct
     4441 ggaccgcttc aagctgatca acgacacgct gggccaccac gtcggcgacc tgctgatcgc
     4501 cgaggtggcg cggcggctgc tgcgctgcgt gcgcgccggc gacaccgtcg ggcgcatcag
     4561 cggcgacgag ttcggcgtgg tgctcgccga tctggcgcac gccgacgacg ccgcgctggt
     4621 gtcgcagaag ctgctcgagg cgctcgccgc gccgtatcag ctggccggca gcgaggccta
     4681 caccagcgcg agcatcggca tcgcggtgtg cccgggcgac ggcacgcacg ccgaggacct
     4741 gctgcgcaac gccgacatgg cgatgtaccg cgccaaggaa tcgacgcgca acgcctactg
     4801 cttcttcacc ccggagatga accagcgcgc ctcggcgcgc atgcagctga acaacgacct
     4861 gcgccacgcc atcgagcggc gcgagttcgt gctgcattac cagcccaagg tggacctggt
     4921 gagcggccgc atgcaggggc tggaggcgct gctgcgctgg aaccaccccg agcgcggcct
     4981 ggtgtcgccg gcggagttca tccccgcgct ggaggattcg ggcctgatcc tgccggtggg
     5041 cgaatgggtg ctggaggagg cctgcgcgca gctcgggcgc tggctggccg ccggcctcgt
     5101 gccggtgccg gtggcgatca acctgtcggc caagcagttc cgccggcgcg acctggacga
     5161 cgtcatcgcg cgcgcgctcg ccgccgccgg ggtgccgagc gcgctgctcg agctcgagat
     5221 caccgagagc agcctgatgg acgatccgga gcaggcggtg cgcctgctcg acagcctgcg
     5281 ccgccagggc gtgcgcactt cagtggacga cttcggcacc ggctactcga gcctgtccta
     5341 cctcacgcgg ctgccgctct cggcgctgaa gatcgaccgc agcttcgtgc gcgacgccat
     5401 gaccaacccg gaggccgcct cgatcgtgcg cgcggtgatc gacatggcgc acaacctgcg
     5461 cttcaccgtg gtcgccgagg gcgtggaaac cgagcagcag gtggcgttcc tgcgccagca
     5521 cgcctgcgac caggcgcagg gctacctcta cggccggccg ggcaccgccg accaggtgcg
     5581 cgggcgcctg gcgcccgcgc tgccgcgcgc atgagcaacg tcgttcccct caagcccggc
     5641 gcggcgggcg ccgtgcggcg catcctgatg gtcgaggacg acgccaacga cgccgagctg
     5701 ctgcaggcct gcctcgccaa ggcctgcccc gagggcagcg tgatcctgca cgcgcgcacg
     5761 ctcgccgagg gcctcgccct gcagcgcgcg cacgaggtgc agctcaccct gctcgacctc
     5821 gacctgcccg acagctcggg cttcggcacc ctggagcgca tgcgcgccgc ggcgagcggc
     5881 ccggtggtgg tggtgtcggg caacagccac ccggcgctgg tggacgaggc gctcgcgcgc
     5941 cgcgcctacg acgtgatccc caaggacgaa ctggacgccg ccacgctgcg ccgcgtgctg
     6001 cgcctggcga ccctgcacca cgaggcgggg cgcgcgctgc gcgccaccga ggggcgcctg
     6061 cgcgccctgg tggagaacag cgccgaggcc ctggtgctgt tcgacgccgc cggccgcatc
     6121 gagtacgcca gcgcctcgct gcgccgcgcg ctgggctacg acgcggccga ggtgatcggc
     6181 cgcaacggcc tgatgttcgt gcaggaggag gaccgccgcg cggtgcagga ggccttcccg
     6241 cggctggtcg ccgccgagga cgcgagcgtc accatgcgcg tgcgcttcgt gcacaaggac
     6301 ggcagcgcgc gcctgctgga ggccacgctg gtcaaccgcc tggccgacgc cgaggtggtg
     6361 gccatcgtgt gcagcttccg cgacctgacg cgcgaagagg agcagcgcgc gcgcttcgac
     6421 gcggccttcg acaacgccgc cgtcggcatg gcgcacgtcg acctggatgg gcgcatcctg
     6481 ctcgccaacc ggcgcctggg cgagatgctg ggctacgcgc gcgaggccct ggccgggcgc
     6541 acgatgttcg agctgtcgca tcccgaggac gccgacgcca ccagcgagga gcgcgcgcag
     6601 ctgcacgccg gcgcgatccc gcatttcgcc aagcgcaagc gctacctgcg cagcgacggc
     6661 ggcgtgctgt gggcgcagat caccgtggcg gcggagcgcg acgcgcaggg cctggtgcgc
     6721 tacgagaccg ccgccttcga ggacgtgggc gagcaggtgc gcgccgagga gcggctgcgc
     6781 gcgagcgagg cgcgcctgcg cgcgatcatc ggcgcggagc ccgaatgcgt gaaggtgctg
     6841 gacgacgccg gcctgctgct cgacatgaac cccgccggcc tcgccatgat cgaggccgac
     6901 agcctggcga cggtggccgg gcagtgcgtg tacaacctgg tgacgccggc ctcgcgcggg
     6961 gccttccgcg ccctcaccga gcgcgcctgc cgcggcgagc gcggcatact cgaattcgag
     7021 gtggtcgggc tgaagggcac gcgccgctgg atggagacgc acgcggtgcc gctgcgcgac
     7081 gagaccgggc gcgcgctgat gctcggcatc acgcgcgaca tcaccgagcg caaggccgcc
     7141 gagctcgcgc tgcgcgagag cgaggcgcgc ttccgcagcc tgaccgagct ttcgtcggac
     7201 tggtactggg agcaggacgc ggaataccgc ttcacgcgct tcgacggcgg cgcgggcgac
     7261 gccgcctggg gcggcgacca gagcaagtcc ctcggcctgc agcgctggga gatcccgggc
     7321 ctgagcccgc tcaactgcac gtgggaggag caccgcgcgc tgctcgatgc gcgcaagccc
     7381 ttccgcggcg tcgagtacct gcgcctcacc gatcagggcg agcggcgcta cgtcaccacc
     7441 agcggcgagc cggtgttcgg cgccgacggc gcattcctcg gctaccgcgg caccgccgcc
     7501 gacatcaccg agcgcaagac cgcggagctc gcggtgcgcg aaagcgagtc gcgcttccgc
     7561 agcctgaccg agctgtcctc cgactactat tgggagaccg acgccgagca ccgcctgagc
     7621 ctgcgcggca gcggcggcac cgcggtggcg cgctccacct tcggcccggg cgggggcgtc
     7681 ggccagcgcc gctgggagct gccgttcaag tcgcccgaca tggacggctg gatggcgcac
     7741 aaggcggtgc tcgaccagca cgagcctttc gccggtttcg aattctcgcg gccgacgccc
     7801 gagggcgggg aacgcttctt ctccatcacc ggcgggccgg tgtacgacgc cgagggcagg
     7861 ttcgccggct accgcggcgt gggcagcgac atcaccgcgc gcaagctcgc cgagcggcgg
     7921 ctgcggctgg agcacgaggt ggcgcgcatc ctggccgctg cgcccgacgc gcgcgccggg
     7981 ctgcagggcg cgatccgcgt cgtctgcgag accgaggtct gggactgcgg ccgctatttc
     8041 gaggtcgacg ccggggccgg gctgctgcgc cacagcgagg cctggggcgt ggacgacccc
     8101 gaggtgcggc gcttcctgga ggattcgcgc ggcatgaccc tggcgcgcgg cgacggcctc
     8161 gccgggcggg tatgggacag cggcgagccg gcctgggtgg ccgatatccg caaggatgcg
     8221 cgcgtgtcgc cgcagggcct ggcgatcatg ggcgagatgc gcggcgcctt cgtgttcccg
     8281 gtgacgctgc attcggggac cgtcggggtg ctcgcgatct ccggccgcga ggtgcgcgaa
     8341 cccgacgcgg cgctgctgcg caccatggcc accgtcggca accagctggg ccagttcctc
     8401 agccgccagc gcgccgagga agcgacgcgc cgcagcgagg agcgcttccg cagcctcacc
     8461 aagatgtcct cggacttcta ctgggagacc gacgccgccc accgcttcac gcagcgcggc
     8521 accggcgcga agaacaccgc ggtgccggcg ttccgcgacg gcaccatgtt cggcaagacg
     8581 cgctgggagc tgcccttcgt caagcccgac atggccggct ggatggcgca caaggcggtg
     8641 ctcgatgcgc acctgccgtt ccgcgacttc gagttctcgc gcctcggcgc ggacggtgcc
     8701 gagcacgcca tcgtcatcag cggcgaaccg gtgttcgacg gcgccgggaa cttcaccggc
     8761 taccgcggcg tgggcagcga catcaccgag cgcaagctcg ccgagcggcg cgtgcacctg
     8821 gagcaccagg tggcgcagct gctggcggcg gcgccggagg cgcgcagcgc gctgcgcggc
     8881 gtgatccgca ccatctgcga gaccgagggc tgggactgcg gccgctactt cgaggtcgac
     8941 gccggggcca acctgctgcg ctacagcgag ggctggggcg tggacgaccc ggaggtgcag
     9001 cgcttcctca gggagacccg cgtgctcacg caccggccgg gcgaaggcgt gcgcggcatg
     9061 gtgtggcaaa gcggcgagcc gatctgggtc gccgacgtgc gcaacgaccc gcgcgtcacc
     9121 gcgcgggcgc aggccacgct gggcgagatc cgcggcgcct tcgtcgcgcc ggtgagcgcc
     9181 ggggcgacgg tggtcggcgt gctgtccatc tccagccgcg tgatgcgcca gcccgacgag
     9241 cggctgctca aggcgatggg cgtgatcggc agccagctcg gccagttcct gcagaagacg
     9301 cgcggcgagc aggcgctgcg cgacagcgaa gagcgcttcc gcaacctgac cgagctgtcc
     9361 tccgactggt actgggagca ggacgccgag ttccgcttca ccaagttcgg catggacgct
     9421 tcctcgccgg tgtacggcac gcagtccgag cccctcggca agacgcgctg ggagctgccg
     9481 ctcacgccgc tctccagcac cttcgagcag caccgcgaga ccctgcgcgc gcaccgcacg
     9541 ttccgcgact tcgagtacag ctacgccggc gccgacggca agacgcgcta cgtcgccgcc
     9601 agcggctacc cggtgcacga cgccgagggg cggttcctcg gctaccgcgg catcgcgcgc
     9661 gacatcaccc cgcgcatgca gtcggaggcg cgcatgcgcg tgcacgccga gcgccaggcg
     9721 gcgatcgccg gcttcggcca gttcgccatc ggccggcgca gcaccgagga gctgtacacc
     9781 gaggcggcgc gcgcgctgcg ctgcgagggc gtggacgcgg tgtgcctgct ggagatgcac
     9841 accgagcggc gcgagttcct ggtgcgcgcg gcgcagggcg agggcccgca cgcctcggtc
     9901 ggcctcaccg gcccggtggc gagcgacagc gtgtggccgg aaatcctgcg cgagaacgcc
     9961 tcgcgcatcg ccgggcgcga gtacctcgcc gggcggccga tcgaccggcc ctggcgcgcc
    10021 tggctgcacg gcatgggcag cgcggtgtac acgccggtgc gcgacgacga gaagcccatc
    10081 gccatgctgt gcatgtacgc ggtgcgcgag caggccttcg gcgcggatga cgtgcgcttc
    10141 gccgagtcca tgagccacgt gctctccacc gcgctgcagc ggcagaaggc cgaggagcgc
    10201 ctggcgcgca tggcgcaatt cgacgcgctc accgggctgc ccaaccgcac cctgctgcag
    10261 gaccggcttg gccagaccat cgtgcagtcg cgccgccggc agctgcacgc cggggtgctg
    10321 ttcgtcgacc tggaccgctt caagctggtg aacgacaccc tcggccacca ccagggcgac
    10381 gcgctgatcc gccaggtggg ccagcgcctg ctcgcctgcg tgcggccggg cgacacggtc
//
LOCUS       DASRIE010000185         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_960002, whole genome shotgun sequence.
ACCESSION   DASRIE010000185 DASRIE010000000
VERSION     DASRIE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960002"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..491)
                     /gene="yidD"
                     /locus_tag="VFB53_07670"
     CDS             complement(162..491)
                     /gene="yidD"
                     /locus_tag="VFB53_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZN86647.1"
                     /translation="MRSLRVLAEAPRRVLIALVRAYRFWLSPWLGSRCLYLPTCSAYA
                     LQALERHGALAGSYLTLARLARCGPWCEAGHDPVPDERPKLFTRLLHPAAAPAEPASS
                     SPDKNIP"
     gene            complement(494..982)
                     /locus_tag="VFB53_07675"
     CDS             complement(494..982)
                     /locus_tag="VFB53_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZN86648.1"
                     /translation="MIGRIERSADFERVLAAPSRARSAHFAVHHLDAAPAAPKRSSKR
                     VIPSLSTELSTGLTTAAAAVVDDGMGGRAWLGAVVPKRHARRAVTRSLLKRQIRAAAA
                     RRPGLPAGLWVVRLRAPFAREQFPSAASRALGAAARTELDGLLDEATRRAAAAPRRPR
                     GD"
     gene            complement(1014..1148)
                     /gene="rpmH"
                     /locus_tag="VFB53_07680"
     CDS             complement(1014..1148)
                     /gene="rpmH"
                     /locus_tag="VFB53_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZN86649.1"
                     /translation="MKRTYQASKVRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            1513..>2739
                     /gene="dnaA"
                     /locus_tag="VFB53_07685"
     CDS             1513..>2739
                     /gene="dnaA"
                     /locus_tag="VFB53_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZN86650.1"
                     /translation="MSADLWQRGCERLAAELPEQQFNTWIRPLPAADVADGVVTVRVP
                     NRFKLDWIRNQYAGRIEAVLTELAGKPVRLEIALAPRENSSAANAPTYPPPGPARPAG
                     AGVSAAAALAGAAGLPALAAVPSSPAAVRELTRHRLNTNLTFDNLVPGRANQMARTAA
                     LHVAGAPGQMYNPLFIYGGVGLGKTHLIHAVGNALLSDKPHARILYLHAEQFITDVVR
                     NYQRKTFDELKAKYHSLDLLLIDDVQFFAGKERTQEEFFNAFEALLAKRAHIIMTSDT
                     YPKGLVDIDERLTSRFDAGLTVAIEPPELEMRVAILIRKADAEGTSMPEDVAFFVAKN
                     VRANVRELEGALRKILAYSRFSHKEVNIQLARDALKDLLSIQNRQISVENIQKTVADF
                     YKIKVADMYSKKRPASI"
BASE COUNT          458 a          970 c          953 g          358 t
ORIGIN      
        1 gggcgcggcg cccggcgccg ggccgttcgc caccgcggcg ggcggcttgg gcggcggcgg
       61 cgcgaagatc gagggctgcc cggtgtgctt gttccacgcg tcgtacagca ggaacagcga
      121 gatcaggaag acaccccaga gcagggtgcg gcgcatgtcg gtcatgggat gttcttgtcg
      181 ggcgaggagg aggccggttc ggccggggcg gcagccggat gcagcaggcg cgtgaagagc
      241 ttgggtcgct cgtcgggcac cgggtcgtgg cccgcctcgc accacggacc gcagcgcgcc
      301 aggcgagcca aggtgaggta gctgcccgcg agcgcgccat ggcgctcgag cgcctgcagc
      361 gcatacgccg agcaggtggg caggtagagg cagcggctgc ccagccacgg gctgagccag
      421 aagcgatagg cgcgcaccag cgcgatcagc acgcggcgcg gcgcttcggc cagcacgcgg
      481 agcgagcgca tggtcagtcg ccgcgcgggc gacgcggcgc ggcggcggcc cgccgtgtcg
      541 cctcgtcgag caggccgtcg agctcggtgc gcgcggcggc gccgagcgca cgcgacgcgg
      601 cgctcgggaa ttgctcgcgt gcgaagggcg cgcgcaggcg caccacccac aagccggcgg
      661 gcagaccggg ccggcgcgcg gcggccgcgc ggatctggcg cttgagcagc gagcgcgtca
      721 cggcgcgccg ggcatggcgc ttgggcacga cggcaccgag ccaggccctc ccgcccatcc
      781 cgtcatccac aacggcggcc gccgcggtgg tcaagcctgt tgacaactcg gtggacaagg
      841 aggggatgac ccgcttggaa cttcgtttcg gcgcggccgg cgcagcgtcc aggtgatgga
      901 cggcgaaatg cgcgctgcgc gcccgcgagg gcgcagcgag cacgcgttcg aagtcggcgg
      961 aacgctcgat gcgtccgatc acgctgcccc cggcgggcga gaccccgagg acgtcagacc
     1021 gcgaggcgct tgcggccctt ggcgcggcgc gcgttgatga ccgcacggcc cccgcgggtc
     1081 ttcatgcgaa cgagaaagcc gtgcgtgcgg gcgcggcgga ctttggaggc ttggtaggtg
     1141 cgtttcatgg tggttcctgc gtggcctgcg gtggggcccg atgcgcctgg aaggctgccc
     1201 gacacgggcg gctccccgaa gcccaggcgc ggcgttccgg tgcgctctgg cgatctcgcg
     1261 cgcctcttgc tgcgcgccca acgagactgc caaaagcatc cccgaagcgc caggcaagcc
     1321 gaaatgcccg cccgccccgc gcatccggga aacccactat tacagcaaaa agggccaacg
     1381 aagtcaaaga cttggacggc agcatgcggc ccggaccggg acttgacccg cgattcgaaa
     1441 ctgtggataa ccacgccctc gacccacccg gggaccgtac aatccggccg ctccaaaaag
     1501 agttttccac aaatgagcgc tgatctctgg caacgcggct gcgagcgtct tgcagccgag
     1561 ctgcccgaac aacaattcaa cacctggatc cgtcccctgc cggctgccga cgtggccgac
     1621 ggcgtggtca cggtgcgggt gccgaaccga ttcaagctcg actggatccg caaccagtac
     1681 gccggccgca tcgaggccgt gctcaccgag ctcgccggca agccggtccg gctggagatc
     1741 gcgctggcgc cgcgcgagaa cagctccgcg gccaacgccc ccacctatcc gccccccggg
     1801 ccggcgcgtc ccgcgggcgc cggcgtttcg gcagctgccg cgctcgccgg tgcggcaggc
     1861 ctgccggcgc tggcggcggt gccgtcctcg ccggccgcgg tgcgcgaact gacgcgccac
     1921 cgcctcaaca ccaacctcac attcgacaac ctggtccccg gccgcgccaa ccagatggcg
     1981 cgcacggccg cgctgcacgt ggccggcgcc ccgggccaga tgtacaaccc gctgttcatc
     2041 tacggcggcg tggggctggg caagacgcac ctcatccatg ccgtgggcaa cgcgctgctg
     2101 tccgacaagc cccacgcccg catcctgtac ctgcacgcgg agcagttcat caccgacgtc
     2161 gtgcgcaact accagcgcaa gaccttcgac gagctgaagg ccaagtacca ctcgctggac
     2221 ctgctgctga tcgacgacgt gcagttcttc gccggcaagg agcgcacgca ggaggagttc
     2281 ttcaacgcct tcgaggcgct gctcgccaag cgcgcgcaca tcatcatgac cagcgacacc
     2341 tatcccaagg gcctggtcga catcgacgag cggctcacct cgcgcttcga cgcggggctc
     2401 acggtggcca tcgagccgcc cgagctggag atgcgcgtgg ccatcctgat ccgcaaggcc
     2461 gacgccgagg gcacctccat gcccgaggac gtggcgttct tcgtcgccaa gaacgtgcgc
     2521 gccaacgtgc gcgagctcga gggcgcgctg cgcaagatcc tggcctactc gcgcttctcg
     2581 cacaaggaag tgaacatcca gctcgcgcgc gatgccctca aggacctgct gtcgatccag
     2641 aaccgccaga tttcggtcga gaacatccag aagacggtgg cagacttcta caagatcaaa
     2701 gtcgccgaca tgtattccaa gaagcgcccc gcctcgatc
//
LOCUS       DASRIE010000186        11306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1745960, whole genome shotgun sequence.
ACCESSION   DASRIE010000186 DASRIE010000000
VERSION     DASRIE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745960"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..477
                     /locus_tag="VFB53_07690"
     CDS             145..477
                     /locus_tag="VFB53_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86651.1"
                     /translation="MAVPWFAVGKLVLDNLDSIIGVVKPVLTRKKVEGLPTQTDLLNQ
                     QIAELQAAASGNAEQIKQLAAQLKEVVAAAEQASANAAMERRILRSLCIAAIAISVVS
                     IIAAMAGG"
     gene            478..933
                     /locus_tag="VFB53_07695"
     CDS             478..933
                     /locus_tag="VFB53_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZN86652.1"
                     /translation="MPAPYPGGCLCGATRYRVTEEPLTVYACHCTDCQKRTGGAFGLS
                     MWVRRSAVEVTKGEAAVQKSLAADGSPRQGRICAQCSTRLWSEPPKHAALAVVRPGTL
                     DDTSWLKPVAHIWTRSAQPWFVFPPDAVKIEKQPTDLREMMTLWKNRAT"
     gene            912..1592
                     /locus_tag="VFB53_07700"
     CDS             912..1592
                     /locus_tag="VFB53_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86653.1"
                     /translation="MEKSRDLSRVLAPLLFAALAACSDLPQRADPASDAPEGQRPRTA
                     EFKDAASYPQALQAWRTPEDVNAWIGAKFQYDMSRAMMLSESQRSRSGPVHIHEAEEF
                     FSAPSGICVDLSRFAVETLQRIAPELKAHYLMIEFSPVSIGGNTLRMHWLAAFTRGGQ
                     HYFFADSKRPGHLAGPYASTAEYIAEYSAYRNRQVVAFRELESYKRKQRTMAAKQKRE
                     QRSPEPAR"
     gene            complement(1589..2746)
                     /locus_tag="VFB53_07705"
     CDS             complement(1589..2746)
                     /locus_tag="VFB53_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZN86654.1"
                     /translation="MRSSSRPEAFFAPCPRGLEAPLAKELASFGARAPEVTSGGVGFS
                     ANWETCYRANLWSRIASRVLWSVAEFDYRSEDDIYAAARAVNWFELFDVNRTLRVYVT
                     AQKSPVKSLEFVTLRVKDAVCDRFRDDVGRRPNVERADPDVRVHVFLDEKRGALYLDT
                     SGEALFKRGWRMQTVEAPLRENLAAGLVMLSGWKFEEPLLDPMCGGATILVEAAAMAR
                     GRAPGMKRSFGFEKLDSFNASLWKALREEASSPAQKAPRLQLFGSDSDATALTAARRN
                     LGAAGVERWVTLEASDVLSRKAPADSGVMIANPPYGERIGESEELSRFYPKLGDALKR
                     NFAGWNCYFFTADRRMEKLIRLSTTRRTVLYNGPIECRLFEFKVVAGSNRP"
     gene            complement(2724..3137)
                     /locus_tag="VFB53_07710"
     CDS             complement(2724..3137)
                     /locus_tag="VFB53_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZN86655.1"
                     /translation="MLWLKSLHIVFMVTWFAGLFYLPRLFVYHALAQDPLSRERFTVM
                     ERKLFWGIMTPGAALTIVFGVWLWLGYGFSGAWLHAKLALVALLLAYHAWCGKLMLDL
                     RAGRNTKSHVWFRWFNEFPVLVLFAVVFLAVFKPA"
     gene            complement(3145..3738)
                     /locus_tag="VFB53_07715"
     CDS             complement(3145..3738)
                     /locus_tag="VFB53_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86656.1"
                     /translation="MRAATRRESVAGPAGRLECAVDEPAGTARGVALIAHPHPLFGGT
                     LDNKVVQTLARAFVELGYAAWRPNFRGVGASESVHDEGRGEVEDLAAVLRHAGAVKPV
                     LAGFSFGAAMQAKLAARVDCERLVLVGVAVTKVELPAVPKGTLLIHGEADETIPLAAV
                     LDWARPQELPVLVVPGADHFFHHKLPALRSAVTANWK"
     gene            complement(3738..4052)
                     /locus_tag="VFB53_07720"
     CDS             complement(3738..4052)
                     /locus_tag="VFB53_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZN86657.1"
                     /translation="MTYYARHVFFCCNQRDPAEGRPSCNAKGASAMRDYAKQRVKELG
                     MAGEGKTRVNQAGCLDRCEEGPCIVVYPEAVWYTYVDKADVDEIVEEHLRHGRIVGRL
                     RI"
     gene            complement(4065..5123)
                     /locus_tag="VFB53_07725"
     CDS             complement(4065..5123)
                     /locus_tag="VFB53_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZN86658.1"
                     /translation="MRGSPLARLLLLVYLGLVAYASLYPLSGWRDHGLSPFEFALAPW
                     PRHVLAVDVLADVLGYMPLGLLAVLAMQPRLRGLRSALAATLLGALLSLALEALQTYL
                     PARVPSNVDLLCNAAGALAGALAGLLALRWTSPLGGWRARAFLPGAQADLGLALLGLW
                     LFMQLNPAMLLFGGADLRDLLAGPAGPARAPELFVAIEALTAAANLAAVGLLASAIAA
                     PGAPVRAMVFALTLAALAVKTLAFAIILRAQHSFAWLTPGAAQGLAAGLAIAMIAVSL
                     PRVARLALAAVLLMAAAVLVNFAPHNPYLAAILKLWQQGHFLNFNGLTRLVSWLWPLA
                     AIAFLIFAAARRRDEALR"
     gene            complement(5132..6556)
                     /locus_tag="VFB53_07730"
     CDS             complement(5132..6556)
                     /locus_tag="VFB53_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZN86659.1"
                     /translation="MLIASVVVYLLVTIAIGLWAAKRVANSKDFLVAGRRLPLYMTVT
                     TVFATWFGAETVLSVSATFTRDGLGGVIADPFGSSFCLVFVALFFARAFYRMDLLTIG
                     DFYRKRFGKPVEVTTSLAITASYLGWTSAQLTALGLVFHVLSGGALTLNEGILLGAAI
                     VLGYTIWGGMWSVAWTDLFQTVVIIVGLWIVALLVADMAGGTGKVIAAAAEAGKFSFW
                     PEGGGKEWLAFAAAWATLAIGSIPQQDVFQRVTSARDESTAVKGTLIGGVVYFAFAFV
                     PIFIAYAALVVDPQLAQLFKSDDAREIQFILPHFIRDHVPVWAQVMFFGALLSAILST
                     ASGALLAPTALFTENVLRPFYGSMGERQFMLTLRIVMVCFTLAALLFGLNSKSTMYEM
                     VQNAYKVTLAGAFVPLVAGLFWKRATAAGAMTSILLGLSTWLLAEAFAADGLWPTQFV
                     GLGFAVLGMVVGSLASQPAHPHPA"
     gene            complement(6550..8034)
                     /gene="ubiB"
                     /locus_tag="VFB53_07735"
     CDS             complement(6550..8034)
                     /gene="ubiB"
                     /locus_tag="VFB53_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HZN86660.1"
                     /translation="MSRLLRILAVALRFRLYRFLPRFARAGDPDRSVRLRRALEALGP
                     IFVKFGQVLSTRRDLLPLDIADELAKLQDQVPPFPAAEAAAEIEKGIGSRIEDVFAEF
                     GSAPVASASVAQVHLARLKDGREVAVKVLRPGVEDAIAKDVALLETAAALVERLWADG
                     RRLKPREVVAEFARHLADEMDLMREAANASQLRRNFADSPLLAVPQVHWEWCSQRVMV
                     MERMHGTPVSQVDALRAGGVDIPQLARAGVEIFFTQVFRDGFFHADMHPGNIFVGTEG
                     AMRGKYVALDFGIMGTLTEVDKNYLAQNFLAFFNRDYRRVAQAHLDAGWVPAATRVDE
                     FEAAVRAVCEPIFARPLKEIYFGKLLLRLFQTSRRFGVEIQPQLVMLQKTLLNIEGLG
                     RELDPDLDLWQTAKPFLERWMGEQVGWRGLVRTLRQEAPYWAATLPQLPRLLHRLLAE
                     DRVGGLQRAMERLAEQNARRNRLLAALAALLATGLILFIIAAQC"
     gene            complement(8031..8594)
                     /locus_tag="VFB53_07740"
     CDS             complement(8031..8594)
                     /locus_tag="VFB53_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZN86661.1"
                     /translation="MTLETPFLLALNRLLDAEPWARERLAPHAGESVELRAPPLPALR
                     FAIAAGGRLAPGGEASLSITLGPEALPALARGEEHFMRSVKIEGNARLAQEVLHLARH
                     LRWDFEEDLSRLLGDAAAHTIAGAVRDFAAWQADAARRLGAAFMEYAVEEGRLLVPRA
                     QFAAHAAAVSRLRDALERLEKRLERLA"
     gene            complement(8649..9485)
                     /locus_tag="VFB53_07745"
     CDS             complement(8649..9485)
                     /locus_tag="VFB53_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HZN86662.1"
                     /translation="MGKVWLGIAALVLGFAVALPDAEARRLGGGRTLGAQRNITAPPP
                     AAAPAKQASPAAPAQQGSRMLPILGGLAIGAFLGAMFADHPIMSTLLTAMLAGFLAFV
                     ALAVFRALRAPQSARAAPQPLQYAGLGNETVAAPPPSQSAGFDARTFGGGAAPRANVP
                     AGFDVAGFLRAAKSNFIRLQIANDNGNLEELREVTTPEMFEALRQDTAAGQQTDVVTL
                     NADLLEVATEGARHWASVRFTGLVREAPGAEPAGFEEVWNLAKPVDGSTGWVLAGIQQ
                     MH"
     gene            complement(9524..10258)
                     /locus_tag="VFB53_07750"
     CDS             complement(9524..10258)
                     /locus_tag="VFB53_07750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86663.1"
                     /translation="MAETDFGFERIAEGEKRARVGGVFDRVAPRYDLMNDLMSLGLHR
                     LWKAFAVGVARVRPGERVLDVASGSGDLARALARRAAPGGEVWVSDINRRMLERGRER
                     LTDAGCLLPAVQCDAERLPFPAASFDCVSVGFGLRNMTRKDAALAEMARVLKPGGRLV
                     VLEFSRIWKPLEKPYDLYSFRLLPWLGGRVAGDAGAYRYLAESIRTHPDQRALAAMME
                     RAGFGAVEYFNLSAGVAAVHRGVKLE"
     gene            complement(10279..10626)
                     /locus_tag="VFB53_07755"
     CDS             complement(10279..10626)
                     /locus_tag="VFB53_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZN86664.1"
                     /translation="MPTPTEIKLHQQSRVLELTFDDGRSFRLPYEYLRVFSPSAEVRG
                     HGPGQEVLQVGKRDIEIRHVEPVGSYAIQPTFSDGHATGIYSWEYLYQLGLEQESRWS
                     GYLRQMEAAGASR"
     gene            complement(10649..>11306)
                     /locus_tag="VFB53_07760"
     CDS             complement(10649..>11306)
                     /locus_tag="VFB53_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86665.1"
                     /translation="RGAERSRVDDAVQACTDIAAIHYYISGSVFRGATSFKELTARQR
                     EEIATFGRVSTRDEQDFGVAQDFLLEHWQLEDESAQGLKMLRRAGTPGRRMAHGQLVG
                     VRPADGKNFQLGQVRWLMANDAGDLYCGVKLLPGLPLAAAVRGVGLGAAEERYVQAVA
                     LAAVPALEAPPTLVTAPGWFKPKRVIEAALGKPLRVRLLEVLERGTDFERLSYEVLPD
                     "
BASE COUNT         1849 a         4057 c         3888 g         1512 t
ORIGIN      
        1 cgtgctagct cttcttctat tcggaccggt ggttgtagtg tctttgtggt ggtggacata
       61 tgcctagcag cgcgttgttg accgacgcct tcagctcgct acgctgcgca tgcggagcgg
      121 caaaacgcga acgttagaga ttccatggcc gtaccctggt tcgccgtcgg aaaactggtc
      181 ctcgacaatc tcgattcgat cattggcgtg gtgaagcccg tgctcacccg gaagaaggtc
      241 gaggggctgc cgacccagac cgatctcctg aaccagcaga ttgctgagct ccaggctgca
      301 gcctcaggca acgcggagca gatcaagcaa cttgccgcgc agctgaagga agtcgtcgcc
      361 gcggccgagc aggcatcggc caatgccgcg atggagcgca ggatcctgcg cagcctgtgt
      421 atcgcagcga tcgcgatctc cgtcgtgtcg atcattgcgg cgatggccgg cgggtagatg
      481 cccgccccct accccggcgg ctgcctctgc ggcgccaccc gctaccgcgt caccgaggaa
      541 cccctcaccg tctacgcctg ccattgcacc gactgccaga agcgcaccgg cggcgcattt
      601 ggcctgtcga tgtgggtacg caggtccgcc gttgaagtga caaaaggcga agcagccgtg
      661 caaaagtcat tggccgccga cggcagcccc cggcagggca ggatctgcgc gcaatgcagc
      721 acgcggctgt ggtcggagcc gccgaagcac gccgccctcg cggtcgtccg cccgggcacg
      781 ctggacgaca cttcctggct caagcccgtc gcgcacatct ggacgaggag cgcgcagccc
      841 tggttcgtct tcccgccgga cgctgtcaag atcgaaaaac agcccacgga cctgcgtgag
      901 atgatgacgc tgtggaaaaa tcgcgcgacc tgagcagagt tctggcgccg ttgctgttcg
      961 ccgccctggc cgcctgcagc gacctgccac agagggccga cccggcttcg gatgcgccgg
     1021 aaggccaacg cccccgcacc gccgaattca aggacgctgc ctcctacccg caggccctgc
     1081 aagcctggcg cacccccgag gacgtcaacg cctggatcgg cgcgaagttc cagtacgaca
     1141 tgtcgagggc gatgatgctc tcggagagcc agcgcagccg ctccggccca gtgcacatcc
     1201 acgaggccga ggagttcttc agcgcgccct cgggcatctg cgtggacttg agccgcttcg
     1261 cagtggagac gctccagcgc attgccccgg agctgaaagc gcattacctg atgatcgaat
     1321 tctctccggt cagtatcggc ggcaacaccc tgcgaatgca ctggctcgcc gcgttcacgc
     1381 gcggcggcca gcactacttc ttcgccgact cgaagcgccc gggacacctg gcgggcccct
     1441 acgccagcac ggcggagtac atcgccgaat actcggccta ccgcaaccgg caggtcgtcg
     1501 ccttccgcga gctcgagtcc tacaagcgaa agcagcgcac catggccgcg aaacagaaac
     1561 gcgaacagcg ctcccctgag ccggcgcgct agggccggtt gcttcccgcc accaccttga
     1621 attcgaacaa ccggcactcg atcggcccgt tgtagagcac cgtgcgccgc gtcgtcgaga
     1681 gccggatcag cttctccatc ctccggtcgg cggtgaagaa gtagcaattc cacccggcga
     1741 agttccgctt cagcgcatcc cccagcttgg gatagaagcg cgacaactcc tcgctctccc
     1801 cgatcctctc gccgtagggc gggttggcga tcatcacgcc ggaatccgcc ggcgccttgc
     1861 gcgagagcac atcgctcgcc tccagcgtca cccagcgctc cacccccgcc gcgccgagat
     1921 tgcgccttgc cgccgtcagc gccgtcgcat ccgaatcgct gccgaagagc tgcagccggg
     1981 gcgccttctg cgccgggctg gaagcctcct cgcgcagcgc cttccacagc gaagcattga
     2041 agctgtcgag cttctcgaag ccgaacgaac gcttcatccc cggcgcccgc ccccgcgcca
     2101 tcgccgccgc ctccaccaga atcgtcgccc cgccgcacat ggggtcgagc aggggctcct
     2161 cgaacttcca gcccgagagc atcaccagcc ccgccgcgag gttctcgcgc aggggcgctt
     2221 ccacggtctg catgcgccag ccgcgcttga acagcgcctc gccggaagta tccaggtaca
     2281 gcgcgccgcg cttctcgtcc aggaacacgt gcacccgcac gtccgggtcg gcgcgctcca
     2341 cgttcggccg gcgccccacg tcgtcgcgga agcggtcgca caccgcgtcc ttcacgcgca
     2401 gggtgacgaa ctccaggctc ttcaccgggc tcttctgcgc ggtgacgtag acgcgcagcg
     2461 tgcggttgac gtcgaacagc tcgaaccagt tcaccgcgcg cgccgcggcg tagatgtcgt
     2521 cttccgagcg gtagtcgaat tcggccacgc tccagagcac gcgcgaggcg atcctcgacc
     2581 acaggttcgc ccggtagcag gtctcccagt tcgccgagaa ccccactccg cccgaagtga
     2641 cttccggcgc gcgcgcgccg aaggatgcga gctccttcgc gagcggcgcc tccagccccc
     2701 gcgggcaggg ggcgaagaaa gcctcaggcc ggcttgaaga ccgcaaggaa cacgaccgcg
     2761 aagagcacca gcaccgggaa ctcgttgaac cagcggaacc agacgtggga tttcgtgttg
     2821 cgcccggcgc gcaggtcgag catcagcttg ccgcaccagg cgtggtaggc gaggagcagc
     2881 gccaccagcg cgagcttggc gtggagccag gcgccggaga acccgtagcc cagccacagc
     2941 cagacgccga acacgatggt gagcgcggcg ccgggggtca tgatgcccca gaagagcttc
     3001 ctctccatca ccgtgaagcg ctccctggac agggggtcct gcgcgagcgc gtggtagacg
     3061 aatagccgcg gcaggtagaa caggcccgcg aaccaggtca ccatgaacac gatatgcagc
     3121 gacttcagcc agagcatgcg cgcctcattt ccagttggcc gtcaccgcgc tcctgagcgc
     3181 ggggagcttg tggtggaaga aatgatcggc ccccggcacc accagcaccg gcagctcctg
     3241 cggccgcgcc cagtcgagca cggcggcgag cggaatggtc tcgtcggcct cgccgtggat
     3301 caggagggtc cccttgggca ctgcaggcag ctcgaccttg gtcaccgcga cgcccaccag
     3361 caccaggcgc tcgcagtcca cccgcgcggc gagcttcgcc tgcatggccg cgccgaagga
     3421 aaacccggcc agcaccggct tcaccgcccc agcgtggcgc agcaccgcgg cgaggtcctc
     3481 gacctcgccg cgcccttcgt cgtgcacgct ctcgctcgcc cccacgccgc ggaagttcgg
     3541 ccgccaggcg gcgtagccca gctcgacgaa tgcgcgcgcc agcgtctgca ccaccttgtt
     3601 gtccagggtg ccgccgaaca gcgggtgcgg gtgcgcgatc agcgcgacgc cgcgcgccgt
     3661 cccggcgggc tcgtccaccg cgcattcgag ccggccggcc ggccccgcga ccgactcgcg
     3721 gcgcgtggcc gcgcgcatca gatcctcaat cgcccgacga tacggccgtg ccgcaggtgc
     3781 tcctcgacga tttcgtcgac gtcggccttg tcgacgtagg tgtaccagac cgcctcgggg
     3841 tacacgacga tgcagggccc ctcttcgcag cggtccaggc aacccgcctg gttgacccgg
     3901 gtcttgccct cgcccgccat gcccagctcc ttcacgcgct gcttggcgta gtcgcgcatc
     3961 gccgaggcgc ccttggcgtt gcagctgggg cggccctcgg cgggatcgcg ctggttgcag
     4021 cagaaaaaca cgtggcgcgc gtaatacgtc atggggggcg gattctaccg cagcgcctcg
     4081 tcgcgccggc gcgcggcggc gaagatgagg aaggcgatgg cggcgagcgg ccacagccag
     4141 gacaccaggc gcgtcaggcc gttgaagttg aggaagtgcc cctgctgcca gagcttgagg
     4201 atcgccgcca ggtaggggtt gtgcggcgcg aagttcacca gcaccgcggc ggccatcagc
     4261 agaaccgcgg caagcgcgag ccgcgccacg cgcggcagcg acaccgcgat catcgcgatc
     4321 gccagccccg cggcgagccc ctgcgcggcg ccgggggtga gccaggcgaa gctgtgctgg
     4381 gcgcgcagga tgatggcgaa ggcgagcgtc ttcaccgcca gcgcggcgag ggtcagggcg
     4441 aacaccatgg cgcgcaccgg ggcgccgggc gccgcgatgg ccgaggcgag gagccccacg
     4501 gcggcgagat tcgcggctgc ggtgagggcc tcgatggcga cgaacagttc gggcgcgcgc
     4561 gccggcccgg ccgggccggc gaggaggtcg cgcaggtccg cgccgccgaa cagcagcatc
     4621 gccggattga gctgcatgaa cagccacagg ccgagcagcg cgaggcccag gtcggcctgc
     4681 gccccgggca ggaaggcgcg cgcgcgccag ccgcccaggg gcgaagtcca gcgcagcgcc
     4741 aggagcccgg ccagcgcgcc ggcgagcgcg cccgcggcgt tgcacaggag gtccacattg
     4801 gagggcacgc gcgccggcag gtaggtctgc agcgcttcca gcgcgagcga caggagcgca
     4861 ccgaggagcg tggcagcgag cgccgagcgc aggccgcgca gccgcggttg catggccagc
     4921 acggcgagca agccgagcgg catatagccc aggacgtcgg cgagcacgtc gaccgcgagc
     4981 acgtggcgcg gccagggcgc cagtgcgaac tcgaagggcg acaggccgtg gtcgcgccag
     5041 ccggacaggg gatagagcga ggcgtaggcg acgagcccca ggtagacgag gaggaggagt
     5101 cgcgcgagcg gcgagccgcg caccggatcg cctaggccgg gtgcgggtgc gccggctgcg
     5161 aggcgagcga ccccaccacc atgccgagaa cggcgaagcc gaggcccacg aactgcgtcg
     5221 gccacagccc gtcggcggcg aaggcctccg ccagcagcca ggtggaaagg ccgagcagga
     5281 tggaggtcat ggcgccggcg gcggtggcgc gcttccagaa caggcccgcc accagcggca
     5341 cgaaagcgcc ggccagcgtc accttgtagg cgttctgcac catctcgtac atggtgctct
     5401 tcgagttcag gccgaacagc agcgccgcca gcgtgaagca caccatcacg atgcgcaggg
     5461 tgagcatgaa ctggcgctcg cccatgctgc cgtagaaggg ccgcagcacg ttctcggtga
     5521 acagcgccgt gggcgcgagc agcgcgcccg aggcggtgga caggatcgcc gagagcagcg
     5581 cgccgaagaa catcacctgc gcccacaccg gcacgtggtc gcggatgaag tgcggcagga
     5641 tgaactggat ctcgcgcgcg tcgtccgact tgaacagctg cgcgagctgc gggtccacca
     5701 ccagtgcggc gtaggcgatg aagatcggca cgaaggcgaa ggcgaagtac accacgccgc
     5761 cgatcagcgt gcccttcacc gcggtcgact cgtcgcgcgc cgaggtgacg cgctggaaca
     5821 cgtcctgctg cgggatcgag ccgatggcga gcgtcgccca ggccgcggcg aaggcgagcc
     5881 actccttgcc gccgccctcc ggccagaagc tgaacttgcc ggcctcggcg gcggcggcga
     5941 tcactttgcc ggtgccgccc gccatgtccg ccaccagcag ggcgacgatc cacagcccga
     6001 cgatgatcac cacggtctgg aagagatcgg tccaggccac cgaccacatg ccgccccaga
     6061 tggtgtagcc cagcacgatc gccgcgccga gcaggatgcc ctcgttcagg gtgagcgcgc
     6121 cgcccgagag cacatggaac accaggccga gcgcggtgag ctgcgccgag gtccagccca
     6181 ggtaggacgc ggtgatcgcc aggctggtgg tcacctcgac cggcttgccg aagcgcttgc
     6241 ggtagaaatc gccgatggtc agcaggtcca tgcggtagaa ggcacgcgcg aagaacagcg
     6301 ccacgaacac caggcagaac gaggagccga agggatcggc gatcaccccg ccgaggccgt
     6361 cgcgcgtgaa ggtcgccgac accgagagca cggtctcggc gccgaaccag gtggcgaaca
     6421 ccgtggtgac ggtcatgtaa agcggcaggc gccgcccggc caccaggaag tccttcgaat
     6481 tggcgacgcg cttggccgcc cacaggccga tcgcgatggt gaccaggaga tagacgacga
     6541 ccgaggcgat cagcattgcg ccgcaataat aaacaagatc agcccggtcg caagcagggc
     6601 ggcgagcgcc gccagcaggc ggttgcggcg cgcgttctgc tccgccaggc gctccatcgc
     6661 cctttgcagg ccgccgacgc ggtcctcggc gagcaggcgg tgcaggaggc gcgggagctg
     6721 cggcagcgtc gccgcccagt agggtgcctc ctgccgcagc gtgcgcacca ggccccgcca
     6781 gccgacctgt tcgcccatcc agcgctcgag gaagggcttg gcggtctgcc acagatccag
     6841 gtccgggtcg agctcgcgcc ccagcccctc gatgttgagc agcgtctttt gcagcatcac
     6901 cagctgcggc tggatctcga cgccgaagcg gcgcgaggtc tggaacaggc gcaagaggag
     6961 ttttccgaag tagatctcct tcagcggccg cgcgaagatc ggctcgcaca ccgcgcggac
     7021 cgccgcctcg aactcgtcca cgcgcgtcgc cgccggcacc cagcccgcgt cgaggtgcgc
     7081 ctgcgccacg cgccggtagt cgcggttgaa gaaggcgagg aaattctgcg ccaggtagtt
     7141 cttgtccacc tcggtgagcg tgcccatgat gccgaagtcg agcgcgacgt acttgccgcg
     7201 catcgcgccc tcggtgccga cgaagatgtt gcccgggtgc atgtcggcgt ggaaaaagcc
     7261 gtcgcggaac acctgggtga agaagatctc gacgccggcg cgcgcgagct gcgggatgtc
     7321 cacgcccccg gcgcgcagcg cgtccacctg cgacaccggc gtgccgtgca tgcgctccat
     7381 caccatcacg cgctgcgagc accactccca gtgcacctgc ggcaccgcga gcaggggcga
     7441 atccgcgaag ttgcgccgca gctggctggc gttggccgcc tcgcgcatca ggtccatctc
     7501 gtcggccagg tgccgcgcga actcggccac cacctcgcgc ggcttcaggc gccggccatc
     7561 ggcccacagc cgctccacca gcgccgccgc cgtctcgagc agcgccacgt ccttggcgat
     7621 cgcgtcttcc accccggggc gcagcacctt caccgccacc tcgcggccgt ccttgaggcg
     7681 cgcgaggtgc acctgggcga ccgaggcgct cgccaccggc gcgctgccga actcggcgaa
     7741 cacgtcctcg atcctgctgc cgatgccctt ctcgatctcc gcggcggctt ccgcggccgg
     7801 gaacggcggc acctggtcct gcagcttggc cagctcgtcg gcgatgtcca gcggcaggag
     7861 gtcgcggcgc gtggacagca cctgcccgaa cttgacgaag atcggtccca gcgcctccag
     7921 cgcccggcgc aggcgcacgg agcgatccgg gtcgccggca cgggcgaagc gcggcaggaa
     7981 ccggtacagc cggaagcgca gcgcgacggc gaggatgcgc agcagccggc tcatgcgagc
     8041 cgctcgaggc gcttttccag gcgctccagc gcgtcgcgca gccgcgacac tgctgccgcg
     8101 tgcgcggcga actgcgcgcg cggcacgagc aggcgccctt cctccaccgc gtattccatg
     8161 aacgccgcgc ccaggcgccg cgcggcgtcc gcctgccacg cggcgaagtc gcgcaccgcg
     8221 ccggcgatgg tgtgcgccgc ggcgtcgccg agcagccggg agaggtcctc ctcgaaatcc
     8281 cagcgcaggt ggcgcgcaag gtgcagtacc tcctgcgcga ggcgcgcatt gccttcgatc
     8341 ttcacgctgc gcatgaaatg ctcctcgccg cgcgccagcg ccggcagcgc ctcgggcccg
     8401 agggtgatgc tgaggctcgc ctccccgccg ggcgccaggc gcccgcccgc ggcgatggcg
     8461 aagcgcagcg ccggcagggg cggcgcgcgc aactccacgc tctccccggc gtggggcgcc
     8521 aggcgctcgc gcgcccaggg ctcggcgtcg agcaggcggt tgagcgccag caggaagggg
     8581 gtttccagcg tcaccataga aacaaaaagg gccaccctag ggtggccctt ctcatgagta
     8641 tagagggatc agtgcatctg ctggatgccg gcgagcaccc accccgtcga gccgtccacc
     8701 ggcttggcga ggttccacac ttcctcgaag cccgccggct cggcgcccgg ggcctcgcgc
     8761 accagtccgg tgaagcgcac gctggcccag tgccgcgcgc cctcggtcgc cacctcgagc
     8821 aggtcggcgt tcagcgtgac cacgtcggtc tgctggccgg cggcggtgtc ctggcgcagg
     8881 gcctcgaaca tctccggcgt ggtgacttcg cgcaactcct ccagattgcc gttgtcgttg
     8941 gcgatctgca ggcggatgaa gttgctcttc gcggcgcgca ggaaccccgc gacgtcgaag
     9001 cccgccggca cgttggctcg cggcgcggcg ccgccgccga aggtgcgcgc gtcgaatccg
     9061 gcggactgcg aaggcggcgg tgcggcgacc gtctcgttgc ccaggccggc gtactgcaaa
     9121 ggctgcggcg cggcgcgcgc gctttgcggc gcgcgcagcg cgcggaacac ggcgagcgcg
     9181 acgaaggcga ggaagccggc cagcatcgcg gtgagcagcg tggacatgat cgggtggtcg
     9241 gcgaacatcg cgcccaggaa tgcgccgatc gccaggccgc cgaggatcgg cagcatgcgg
     9301 ctgccctgct gcgcgggcgc ggcggggctc gcctgcttgg cgggcgcggc ggccggcggc
     9361 ggggcggtga tgttgcgctg cgcgccgagc gtgcggccgc cgcccaggcg gcgcgcttcc
     9421 gcatcgggca gcgccaccgc gaaacccagg accaatgccg caattcccag ccagaccttg
     9481 cccatgactt gcgctccccc cttcatcagt tcgtccagcg atgctactcc agtttcacgc
     9541 cgcgatgcac ggccgcgacg cccgcgctga gattgaaata ttcgaccgcg ccgaagccgg
     9601 cgcgttccat catcgcggcc agcgcgcgct ggtcggggtg cgtgcggatc gactcggcga
     9661 ggtagcggta ggcgccggcg tcgccggcga cgcgcccccc cagccaggga aggaggcgga
     9721 aggagtaaag gtcgtagggt ttctccaggg gcttccagat cctggagaac tccagcacca
     9781 ccaggcggcc cccgggcttg agcacccgcg ccatctcggc gagcgccgcg tccttgcgcg
     9841 tcatgttgcg cagcccgaag cccacgctca cgcagtcgaa gctcgccgcc ggaaacggca
     9901 ggcgttcggc gtcgcactgc acggcgggca ggaggcagcc ggcgtcggtg aggcgctcgc
     9961 gcccccgctc gagcatgcgg cggttgatgt cgctgaccca cacttcgccg ccgggcgcgg
    10021 cgcggcgcgc cagggcgcgg gcgaggtcgc cgctgccgga ggcgacatcc agcacccgtt
    10081 cccccggccg cacgcgcgcc acgcccacgg cgaacgcctt ccagagccgg tgcaggccca
    10141 gggacatcag gtcgttcatc aggtcgtagc ggggcgccac acggtcgaat acgccgccca
    10201 cgcgcgcgcg cttctcgccc tcggcgatgc gctcgaaacc gaagtcggtt tcagccatga
    10261 agtgagcgca ggctttctct aacggctagc acctgcggcc tccatttgcc tcaaatagcc
    10321 agaccagcgc gattcctgct cgaggcccag ctggtacagg tattcccagg aatagatccc
    10381 ggtcgcatgt ccgtccgaga aggtcggctg gatggcgtag gagcccacgg gctcgacgtg
    10441 gcggatctcg atgtcgcgct tgccgacctg cagcacctcc tggccggggc cgtgcccgcg
    10501 cacctccgcc gagggcgaaa acaccctcag gtactcatag ggcagccgga agctgcggcc
    10561 gtcgtcgaag gtcagctcca ggacccgcga ctgctggtgc agcttgatct cggtcggcgt
    10621 gggcatggcg cgacgatgat accccgcgct agtcgggcag cacctcgtag gacaggcgct
    10681 cgaagtcggt gccgcgctcg agcacttcga gcaggcgcac gcgcagcggc ttgccgagcg
    10741 ccgcctcgat cacgcgcttg ggcttgaacc agcccggcgc ggtgaccaga gtgggcggcg
    10801 cctcgagcgc cggcaccgcg gcgagcgcca cggcctgcac atagcgctcc tcggcggcgc
    10861 ccaggccgac gccgcgcacc gcggccgcca gcggcagccc gggcagcagc ttcacgccgc
    10921 agtacaggtc gcctgcgtcg ttggccatca gccagcgcac ctgcccgagc tggaaattct
    10981 tgccgtcggc gggccgcacc cccaccagct ggccgtgcgc catgcgccgc cccggcgtgc
    11041 cggcgcggcg cagcatcttc aggccctgcg ccgattcgtc ctccagctgc cagtgctcga
    11101 ggagaaagtc ctgcgccacg ccgaaatcct gctcgtcgcg cgtgctgacg cggccgaagg
    11161 tggcgatctc ctcgcgctgc ctcgcggtga gctccttgaa gctggtggcg ccgcggaaca
    11221 cgctgccgga gatgtagtag tggatcgccg cgatgtcggt gcaggcctgc accgcgtcgt
    11281 cgacgcgcga gcgctcggcg ccgcgc
//
LOCUS       DASRIE010000187         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3054695, whole genome shotgun sequence.
ACCESSION   DASRIE010000187 DASRIE010000000
VERSION     DASRIE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054695"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFB53_07765"
     CDS             <1..192
                     /locus_tag="VFB53_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HZN86666.1"
                     /translation="YKLAWDLIGSEFAGRHLQYEKFYAGASFIVRNHSFREAPWEGFH
                     KVVDDLMGTYDVPVRRVEG"
     gene            189..1625
                     /locus_tag="VFB53_07770"
     CDS             189..1625
                     /locus_tag="VFB53_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZN86667.1"
                     /translation="MRQFFAGVLFAAWVAAATAQPQTPMAFIDAQIAARPGQSGAYVL
                     ETGAHALISRYWLADHARESIEVQYFIWSTDNIGILAAEALLRAADRGVRVRVLVDAL
                     LIDAPDVSLVALARHPKVEIRIYNPKRLWSLVGDFRGYNQRMHDKTLIVDGRIAITGG
                     RNMAAEYYDYNREYNFRDRDVLLLGAVVKDMTTSFERFWASELAVPVEQLHEGKRLDD
                     QDVSRVYGELHEYARAPANFAPEVRAAIAAAPAAFPGIARDIAWGKVDFISDAPGKNT
                     SRGLRGGGATATALAAMVENARTRVVIQSPYLVPSERAFTLLREARARGVKVRISTNS
                     LASTDNIQAFSGYRNQRSRLLRMGIDVYEYRPDPEVQRELVAALPSGQRKPPIFSLHA
                     KTMVVDGAILYVGTFNFDPRSENLNTEVGVIIENRALAAAVEGAIEIDMRPANSWNAS
                     TDDPDSHVSLLKRGRTRFWQFVPIKPLL"
     gene            1678..>2917
                     /locus_tag="VFB53_07775"
     CDS             1678..>2917
                     /locus_tag="VFB53_07775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN86668.1"
                     /translation="MGAALPLPQADRLTAAPAVVVLRGAQASGEIVAAWDDLAAHAIE
                     PNPFYESWMLRPALEWLAAGRDIRLVTVWHDGKLGALLPLERKASYKGLPVGTLRSWR
                     HRHCMLCTPLVRAGGAEGYLAAMLEWLRRAASVVELDYMPADGPAYRLLAQMLPAAQV
                     WEGYERALFRRARDADTYIASAVSRQDRQEVRRRERRLAERGAVAHLTLRPGDPVERW
                     LEELMQLEAGGWKGKQGSALASNEANRRFAAEIFSAAFRRGRLHMVGLDLDGKPLSRC
                     SSILAGDGGYAFKTAYDEAFSRFSPGIIAELDRIRALHELPNVQWMDSFTADGNVVLN
                     RLWNQRVKIRGLTFATGPRGRLALGVREALRRARRWLQRRRTKASAASTSAMPASMVS
                     VTASPSSSQPQSTPSTGTARP"
BASE COUNT          460 a         1006 c         1014 g          437 t
ORIGIN      
        1 tacaagctcg cctgggacct gatcggctcg gagttcgccg gccggcacct gcagtacgag
       61 aagttctacg ccggcgcgtc gttcatcgtg cgcaaccatt cgttccgcga ggcgccctgg
      121 gagggctttc acaaggtggt cgacgacctg atgggcacct acgatgttcc cgtgcggcgg
      181 gtcgagggat gaggcagttc ttcgccggcg ttctcttcgc cgcctgggtc gcagccgcga
      241 ccgcgcagcc gcagacgccg atggcgttca tcgacgcgca gatcgcggcg cgtccggggc
      301 agagcggcgc ttacgtgctg gagaccggcg cgcacgccct gatctcgcgc tattggctcg
      361 ccgaccatgc gcgggagtcg atcgaagtgc agtactttat ctggagcacc gacaacatcg
      421 gcatcctcgc ggccgaggcg ctgctgcgcg ccgccgaccg cggggtgcgc gtgcgcgtgc
      481 tggtggacgc gctgctgatc gacgcgcccg acgtctcgct ggtcgcgctg gcgcgccacc
      541 ccaaggtgga gatccgcatc tacaacccga agcgcctgtg gagcctggtg ggggatttcc
      601 ggggctacaa ccagcgcatg cacgacaaga cgctgatcgt ggacggccgc atcgccatta
      661 ccggcgggcg caacatggcg gcggagtact acgactacaa ccgcgaatac aacttccgcg
      721 accgcgacgt gctgctgctc ggcgcggtcg tcaaggacat gacgacgagc ttcgagcgct
      781 tctgggcgag cgagctggcg gtgccggtgg agcagctgca cgagggcaaa cgcctcgacg
      841 atcaggacgt gagccgcgtc tacggcgagc tccacgagta cgcgcgagca cccgcgaact
      901 tcgcgcccga agtgcgcgcg gcgatcgccg ccgcgcctgc ggcattcccc gggatcgccc
      961 gcgacattgc gtggggcaag gtggatttca tcagcgatgc gccgggaaag aacaccagcc
     1021 gcggcctgcg cggcggcggc gccaccgcga cggcccttgc cgcgatggtg gagaacgcgc
     1081 gcacgcgcgt ggtcatccag tcgccgtacc tcgtgccgtc ggagcgcgcc ttcacgctgc
     1141 tgcgggaagc gcgcgcccgg ggcgtgaagg tgcgcatcag caccaactcg ctcgcctcca
     1201 ccgacaacat ccaggccttc agcggctatc gcaaccagcg ctcgcggctg ctccgcatgg
     1261 gaatcgacgt ttatgagtac aggcccgacc ccgaggtgca gcgcgagctg gtggcggcct
     1321 tgccgagcgg gcagcgcaag ccgccgatat tttcgctcca cgccaagacc atggtggtgg
     1381 acggcgccat cctctacgtc ggcactttca acttcgatcc gcgctcggag aacctgaaca
     1441 ccgaagtggg ggtgatcatc gagaaccggg cgctcgccgc ggcggtggaa ggcgcgatcg
     1501 agatcgatat gcggcccgcc aacagttgga acgcgtccac cgacgatcct gacagccatg
     1561 tcagcctgct caagcgcggc cgcacgcggt tctggcagtt cgttccgatc aagcccctgc
     1621 tctaagcgct agcgctggaa gaatcgcttt taacaaacac cctgtagaaa ggcgggggtg
     1681 ggtgccgccc tcccgttgcc gcaagccgac aggctgactg ccgcgccagc ggtcgtcgtc
     1741 ctgcgcgggg cgcaggcgag cggcgagatc gtcgccgcct gggacgacct ggccgcgcac
     1801 gcgatcgagc caaacccgtt ctacgaatcg tggatgctgc ggccggcgct cgagtggctg
     1861 gctgccggcc gggacatccg gctggtcacc gtctggcacg acggcaagct gggcgcgctg
     1921 ctgccgctgg agcgcaaggc gagctacaag ggcttgccgg tgggcacttt gcgctcctgg
     1981 cggcatcgtc actgcatgct ttgcactccg ctggtgcgcg ctggtggtgc ggaaggctac
     2041 ctcgccgcca tgctcgagtg gttgcgccgt gccgcctcgg tggtggagct cgactacatg
     2101 ccggccgacg ggccggccta tcgcctgttg gcgcagatgc ttcccgccgc gcaggtgtgg
     2161 gagggatacg agcgcgcgct tttccgtcgc gccagggatg ccgacaccta tatcgcgagc
     2221 gcggtgtcgc gccaggaccg ccaggaggta cgccgccgcg agcgccgcct ggccgagcgc
     2281 ggcgcggtgg cgcacctcac gctgcgcccc ggcgacccgg tcgagcgctg gctggaagaa
     2341 ttgatgcagc tcgaggccgg cggctggaag ggcaagcagg gcagcgcgct cgccagcaac
     2401 gaggccaacc gccgcttcgc agccgagatc ttcagcgccg ctttccgccg cgggcgcctg
     2461 cacatggtcg ggctcgacct ggacggcaag ccgctctcgc gctgctcgag catcctcgcc
     2521 ggcgacggcg gctacgcgtt caagaccgcc tacgacgagg ccttctcgcg cttctcgccc
     2581 ggcatcatcg cggagctgga ccgcatccgc gccctgcacg agctgccgaa tgtccagtgg
     2641 atggattcgt tcaccgccga cggcaatgtc gtgctcaacc gcttgtggaa ccagcgcgtg
     2701 aaaatccggg gcctgacctt cgccaccggg ccgcggggca ggctcgcgct gggcgtgcgc
     2761 gaggcgctac gccgcgcccg gcgctggctt cagcgccgca ggaccaaagc gagcgcggcg
     2821 agcaccagcg ccatgccggc gagcatggtg agcgtcaccg cctcgccgag cagcagccag
     2881 ccccagagca cgccgagcac cggcaccgcc aggccga
//
LOCUS       DASRIE010000188         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3295163, whole genome shotgun sequence.
ACCESSION   DASRIE010000188 DASRIE010000000
VERSION     DASRIE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3295163"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFB53_07780"
     CDS             complement(<1..608)
                     /locus_tag="VFB53_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZN86669.1"
                     /translation="MNTQTQARRLVRAGWLIVLGAVLPLGAWMALAPLSMAVVAPAFV
                     KVDLNRRPVQHLEGGIVRSVLVRDGERVSAGQPVLILGDVGVDADRNRLTYRAEVERA
                     GIARLDAEQMRAATLAFPPALVAAARRDARVQEALAKEQALFRTRRDALASETVLLAA
                     QRERIEQEIAALRDQIAQVQASLDLQKSELGQQRKLLKDGFIS"
     gene            complement(608..2290)
                     /locus_tag="VFB53_07785"
     CDS             complement(608..2290)
                     /locus_tag="VFB53_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZN86670.1"
                     /translation="MTKLWQELRPLLGSLAGLSFFINLLYLVPAVFMMQVFDRVLPSN
                     SQETLLVLLGGTMVALLLLLLIDYVRTRLQHVVGGLVEERLSPPVVSAVVAAAARAGQ
                     GGAADGVRDVTALRTLFSANGLIALFDAPWVVFYVAVIWAFHPALGMGAALAALVMVG
                     LAWLNDRLSRRSLDRLQQEGRRASHYVESSMRNAEVLQALGMTGHLLARWRELQGRIA
                     AEQASTGRTSVAFATGTKILRQSIQIFMLSLGAYLVLSQLASPGIMIATTILLGRAVQ
                     PVEQLVGSWRLLAEARSAYRRLLELSAAFERGEPRVALPRPQGQLLIESLLYRVPGSE
                     KAIVSGVALSLAPGEALAVIGPSAAGKSTLARLITGIWAPTAGCVRLDGADVATWPRE
                     ALGPSIGYVPQDVELFDGSVADNIARLGKVDSEKVVAAAKRANAHELILGLAQGYDTQ
                     VGPQGSRLSPGQRQRVALARALYGEPKLLVLDEPNSNLDGDGEVALAQALGAVRKEGV
                     TAIVVTHRPSLIAHVDKILVLAAGRVQQYGPAAQVMKAMREQAQSAVAARAA"
     gene            complement(2287..3192)
                     /locus_tag="VFB53_07790"
     CDS             complement(2287..3192)
                     /locus_tag="VFB53_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86671.1"
                     /translation="MKQALALLVCVAAGLAQAQGLGEVALAPEGDAPGGPEALAYDGR
                     HIWVARPFADTVVRISASSGAHAGSFSVERPGAVLFALSAIWVANTRAHSVVKLGPGD
                     GKVLGTYPVGHLPAAMVSDGEHLWVANSGSNSVMKLDRNGRRLLTVGTQARPLALAFD
                     GANVWVANNKARSVSKLRASDGAVLGVFPAGDGPSALAFDGTDIWVANYFSGNVMKLR
                     AADGAALGTYPTAHGAAGLAFDGHNLWVTSTSTNRVLALRRDGSVMATYATGQRPHQV
                     VFDGANIWLANAASDSVSSTALGWR"
     gene            complement(3189..3704)
                     /locus_tag="VFB53_07795"
     CDS             complement(3189..3704)
                     /locus_tag="VFB53_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZN86672.1"
                     /translation="MSQSSREAIRKRYLPDIVVQTHEGKHALFYEDLVKDRIVVFNFM
                     YLSCEDTCPTTLHTLMEVHTLLRPRIGRDLFMYSITLDPERDSVAALRKEARSHGVRP
                     GWLFLRAAPADTELLRRKLGFVSSDPKVDANKSAHAAMVRFGNEPRQLWSMVSASASP
                     EAIARAIRWVV"
     gene            complement(3704..>4938)
                     /locus_tag="VFB53_07800"
     CDS             complement(3704..>4938)
                     /locus_tag="VFB53_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZN86673.1"
                     /translation="IANATFYDQHYPNVLAGFSVAPTAPNGDLNESLSTLWYHDHRVG
                     FTAQNVYKGLAGFYLLYNHLDGGDETTGFRLPGVRDPNDFYAPVQYDIPLMLADRLFD
                     VESGAMFFDLFNRDGIIGDKFLVNGKVQPYLEVEPRRYRFRILDGGPSRFYQLFLTDQ
                     DANTAIPFWQVSNDGNLLPKPLKVQSLTLSVAERADVVIDFSPWAGKTLYFENRLNQS
                     DGKGPDANLGLPMATAPAGQGQFILQFRVGTQAVDDFSHDLETTSPSYYALPSRPLPR
                     ISRSFRFERELDQWVVNGKPFPDSADVVHFRVKKNSAEQWTIWNTSGGWMHPIHIHFE
                     EFRMISRNGAAIGPGNVEYSRKDVMRLQHGESNVAAWRFRDFEGRYVIHCHNTIHEDH
                     AMMMRFDIDATGDTKQVP"
BASE COUNT          719 a         1731 c         1751 g          737 t
ORIGIN      
        1 gagatgaaac cgtccttgag cagcttgcgc tgctgcccga gctcgctctt ctgcaggtcg
       61 agcgaggcct gcacctgcgc gatctggtcg cgcagcgccg cgatctcctg ctcgatgcgc
      121 tcgcgctgcg ccgcgagcag cacggtctcg ctcgcgagcg cgtcgcgccg ggtgcggaac
      181 agcgcctgct ccttggcgag cgcttcctgc acgcgggcgt cgcgccgggc ggcggcgacg
      241 agcgctggcg ggaaggcgag cgtggcggcg cgcatctgct cggcgtcgag gcgcgcgatg
      301 ccggcgcgct cgacctcggc gcgataggtc agccggttgc ggtcggcgtc gacaccgacg
      361 tcacccagga tcagcaccgg ctggccggcg ctcacgcgct cgccgtcgcg caccagcacg
      421 ctgcgcacga tgccgccttc gaggtgctgc accggacggc ggttgaggtc caccttgacg
      481 aacgccggcg ccaccaccgc catcgagagc ggcgcgagcg ccatccaggc gccgagcggc
      541 agcaccgcgc cgagcacgat cagccatccc gcgcgcacca ggcgcctggc ctgggtttgc
      601 gtattcatca ggccgccctc gcggcgaccg cgctctgcgc ctgctcgcgc atcgccttca
      661 tcacctgcgc cgccggcccg tactgctgca cccgtccggc ggcgagcacc aggatcttgt
      721 cgacgtgcgc gatgagcgac ggccggtgcg tgaccacgat cgcggtcacg ccttccttgc
      781 gcaccgcgcc cagcgcctgc gccagcgcga cctcgccgtc gccgtcgagg ttcgagttcg
      841 gctcgtccag caccagcagc ttcggctcgc cgtagagcgc gcgcgcgagc gcgacgcgct
      901 ggcgctggcc gggcgagagg cgcgagccct gcgggccgac ctgggtgtcg tagccctgcg
      961 ccaggccgag gatcagctcg tgcgcattgg cgcgcttcgc cgccgccacc accttttctg
     1021 aatcgacctt gccgaggcgc gcgatgttgt ccgccacgct gccgtcgaac agctccacgt
     1081 cctgcggcac gtagccgatc gacgggccga gcgcctcgcg cggccaggtc gcgacgtcgg
     1141 cgccgtccag gcgcacgcaa ccggcggtcg gcgcccagat gccggtgatc aggcgcgcga
     1201 gcgtggactt gcccgcggcg ctcgggccga tcacggcgag cgcttcgccc ggcgccaggc
     1261 tgagcgcgac gccggagacg atggccttct ccgagccggg gacgcgatag agcaggctct
     1321 cgatcaggag ctggccctgc ggcctgggca gcgcgacgcg cggctcgccg cgctcgaagg
     1381 cggcggacag ctcgagcagc cggcggtagg cggaacgcgc ttcggccagc aggcgccagc
     1441 tgccgaccag ctgctcgacc ggctgcaccg cgcggccgag caggatggtg gtggcgatca
     1501 tgatgccggg cgaggcgagc tggctcagca ccaggtaggc gccgagcgag agcatgaaga
     1561 tctggatcga ctggcgcagg attttcgttc cggtggcaaa ggcgacgctg gtgcgcccgg
     1621 tgctcgcctg ctcggccgcg atgcgcccct gcagctcgcg ccagcgcgcg agcaggtgcc
     1681 cggtcatgcc gagcgcctgc agcacctccg cgttgcgcat cgagctttcc acatagtgcg
     1741 aggcgcgccg gccttcctgc tgcagccggt ccaggctgcg ccggctgagg cggtcgttca
     1801 gccaggcgag gccgaccatc accagcgcgg cgagcgccgc gcccatgccg agcgccggat
     1861 ggaacgccca gatcacggcg acataaaaga ccacccacgg cgcgtcgaac agcgcgatca
     1921 ggccgttggc ggaaaacagg gtgcgcagcg cggtcacgtc gcgcacgccg tcggcggcgc
     1981 cgccctggcc ggcgcgcgcg gcggcggcga ccaccgcgct caccaccggc ggtgagagcc
     2041 gctcctcgac cagcccgccg accacgtgct gcaggcgcgt gcgcacgtag tcgatcagca
     2101 ggagcagcag cagcgcgacc atggttccgc cgagcagcac gagcagcgtt tcctggctgt
     2161 tgctcggcag cacgcggtcg aacacctgca tcatgaagac cgcgggtacc agatacagca
     2221 ggttgatgaa gaacgacagc ccggcgaggc tgccgagcag cgggcgcagc tcctgccaca
     2281 gcttcgtcat cgccacccca gcgcggtgga tgacaccgag tcgctcgccg cgttggcgag
     2341 ccagatgttg gcgccgtcga acaccacctg gtgcgggcgc tgcccggtgg cgtaggtcgc
     2401 catcaccgag ccgtcgcgcc gcagcgccag cacgcggttg gtgctcgtgc tagtcaccca
     2461 caggttgtgg ccgtcgaacg ccaggccggc cgcgccgtgc gcggtcgggt aggtgccgag
     2521 cgccgcgccg tcggccgcgc gcagcttcat cacgttgccg ctgaagtagt tcgccaccca
     2581 gatgtcggta ccgtcgaacg cgagcgccga aggcccgtcg ccggcgggaa acacgccgag
     2641 cacggcgccg tcgctcgcgc gcagcttgct cacgctgcgc gccttgttgt tcgccaccca
     2701 cacgttcgcg ccgtcgaacg cgagcgcgag cggccgcgcc tgcgtgccga ccgtgagcag
     2761 cctgcgcccg ttgcggtcga gcttcatcac gctgttgctg ccgctgttcg ccacccacag
     2821 gtgctcgccg tcggacacca ttgccgccgg caggtggccg accgggtagg tgccgaggac
     2881 cttgccgtcc ccggggccga gcttcaccac gctatgcgcg cgcgtgttgg ccacccagat
     2941 cgcggaaagc gcgaacagca ccgcgcccgg ccgctcgacg ctgaagctgc cggcgtgggc
     3001 gccgctcgag gcgctgatgc gcaccaccgt atcggcgaac gggcgcgcca cccagatatg
     3061 ccggccgtcg taggcgagcg cttccggtcc gccgggcgca tcgccctccg gggcgagggc
     3121 gacttccccc aagccttgcg cctgggcaag gcccgccgcc acgcacacca ggagggcgag
     3181 cgcctgcttc ataccaccca gcggatggcg cgcgcgatcg cttcgggcga agcggacgcc
     3241 gagaccatcg accagagctg gcgcggctcg ttgccgaagc gcaccatggc cgcgtgcgcc
     3301 gacttgttgg cgtcgacctt cgggtcgctg ctgacgaagc cgagcttgcg gcgcagcagc
     3361 tcggtgtccg ccggcgcggc gcgcaggaac agccagcccg ggcgcacgcc gtggctgcgc
     3421 gcttccttgc gcagcgcggc cacgctgtcg cgctccggat cgagcgtgat cgagtacatg
     3481 aagagatcgc gcccgatgcg cggcctgagc agcgtgtgca cctccatcaa cgtgtgcagc
     3541 gtggtcgggc aggtgtcctc gcagctcaga tacatgaagt tgaagaccac gatcctgtcc
     3601 ttgaccaggt cctcgtagaa cagcgcgtgc tttccctcgt gggtctgcac cacgatgtcc
     3661 ggcaggtagc gcttgcggat cgcctcgcgg ctcgactggc tcatcacggc acctgtttgg
     3721 tgtcgccggt ggcgtcgatg tcgaagcgca tcatcatcgc gtggtcctcg tggatcgtgt
     3781 tgtggcaatg gatcacgtag cggccctcga agtcgcggaa gcgccacgcg gcgacgttgc
     3841 tctcgccgtg ctgcaggcgc atcacgtcct tgcgcgagta ctcgacgttg cccggcccga
     3901 tggccgcgcc gttgcgcgag atcatgcgga actcctcgaa gtggatgtgg atcgggtgca
     3961 tccagccgcc cgaggtgttc cagatcgtcc actgctcggc gctgttcttc ttcacccgga
     4021 agtgcaccac gtcggcgctg tcggggaacg gcttgccgtt caccacccac tggtcgagct
     4081 cgcgctcgaa gcggaacgag cggctgatgc gcggcagcgg gcgcgacggc agcgcgtaat
     4141 acgacgggct ggtggtctcc aggtcgtggc tgaagtcgtc caccgcctgc gtgccgacgc
     4201 ggaactgcag gatgaactgg ccctgtccgg caggcgcggt cgccatcggc aggccgaggt
     4261 tggcgtcggg ccccttgccg tccgactggt tgaggcggtt ctcgaagtag agcgtcttgc
     4321 ccgcccacgg gctgaagtcg atcaccacgt cggcgcgctc ggcgacgctc agcgtgaggc
     4381 tctgcacctt gagcggcttg ggcagcaggt tgccgtcgtt cgacacctgc cagaacggga
     4441 tcgcggtgtt ggcgtcctgg tcggtgagga acagctgata gaagcgcgag ggcccgccgt
     4501 cgaggatgcg gaagcggtag cggcgcggct cgacctcgag atacggctgg accttgccat
     4561 tgaccaggaa cttgtcgccg atgatgccgt cgcggttgaa caggtcgaag aacatcgcgc
     4621 ccgactcgac gtcgaacagg cgatcggcga gcatcagcgg gatgtcgtac tgcaccggcg
     4681 cgtagaagtc gttgggatcg cgcacccccg gcaggcggaa accggtggtc tcgtcgccgc
     4741 cgtccaggtg gttgtagagc aggtaaaagc ctgccaggcc cttgtagacg ttctgcgcgg
     4801 tgaagccgac gcggtggtcg tggtaccaga gcgtgctcag cgactcgttg aggtcgccgt
     4861 tcggcgcggt cggcgcgacg ctgaagccgg cgagcacgtt cggatagtgc tggtcgtaga
     4921 acgtggcatt cgcgatcg
//
LOCUS       DASRIE010000189         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1501452, whole genome shotgun sequence.
ACCESSION   DASRIE010000189 DASRIE010000000
VERSION     DASRIE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501452"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFB53_07805"
     CDS             <1..555
                     /locus_tag="VFB53_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HZN86674.1"
                     /translation="GGDQREPYLETDRPQPLQVYGATRLAGENLALAAAPQRAVVIRT
                     CGLYGTAGSRSKGGNFVDGRVADGRAGKRIEMASEQTVAPTSTHDLSRAVHALLQHPK
                     LAPGLYHLVNEGSCTWYELTRAIVETLRLPAEVVPVDRGGRSGAMRRPLYSVLANTRA
                     RALGIRLRPWREALVDYLRAKYPS"
     gene            555..1436
                     /locus_tag="VFB53_07810"
     CDS             555..1436
                     /locus_tag="VFB53_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86675.1"
                     /translation="MADVGVVHLVREANGLAPLERFIASYREQPAGHAHELILVCKGF
                     PGRLPAEYERALEGVAYRTLHVADRGFDLAPYFAATRRFEHRYFCFLNSFSRPLAAGW
                     LEILHRCASAPGVGLVGATGSYQSFAQSSEERRRLGTRARLRHVARGGSPAMVAQRTG
                     AWVLGGLGLWKPARHFPAFPNYHIRTNAFMASKDVLLRIRVRPILFKLSAFLLESGRS
                     GLTRQVMAMGLRALVVDRAGRAFEKESWHLANTFRQSRQEDLLVEDNQTVAYEHAQPE
                     ERGNLSRIAWGEFARPA"
     gene            1464..2315
                     /locus_tag="VFB53_07815"
     CDS             1464..2315
                     /locus_tag="VFB53_07815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN86676.1"
                     /translation="MRPYLRTRDLNRDASDEEFRYERCPDCGLASLANAPADLGRYYA
                     AGYHEIPASAEGLERGAAHDSYKIDLVRQFATRGRLVEIGPGWGAFCLLARRAGFEVE
                     AIERDPRCCEFLQKELGVRAIRSDDPATALAQAATADAIVLWHVVEHLADPWAVLEAA
                     ARKLAPGGVLLIATPNPESFQLGLFGRFWTHVDAPRHLHLIPVRLLRSRMARLGLDAE
                     LATTTDPGSIGWNDFGWRFSLANLAPHRALKRPLRLAGRVLGKLAALIDNREGRGSAY
                     TAVFRKR"
     gene            2312..3460
                     /locus_tag="VFB53_07820"
     CDS             2312..3460
                     /locus_tag="VFB53_07820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN86677.1"
                     /translation="MKFSILLPTRERLELLRHAVESVRAQDDADWEVVISDNASSQDV
                     AGYVAALGDARVRYSRTDQLLPVTQNWNRALEASRGEYLIMLGDDDALLPRCLSRARA
                     LLAQWRDPDLVYTQALQYAYPGVVPGAAEGFVQVGYNAFLTQGATRPFVLAPETAHTM
                     VREAMRFRIRYGFNAQHFIFSRRLVDALRAKGPFFQSPYPDYYAANAMLLAARNIVAN
                     PEPLVLIGISPKSFGFYHHNRREGEGVDFLQNFPSPEMRARLAPTWVPGSNMNDSWLC
                     AMETLAMNFPERALSVNYRRYRLLQYQATLREHGLRAALRHVRWWEPLLYAPAVLAFG
                     FAYLLPRGLRSRVQDKVKAMFSAYPRFDAQRRTVPYRDILEAARGVSR"
     gene            3508..>3994
                     /locus_tag="VFB53_07825"
     CDS             3508..>3994
                     /locus_tag="VFB53_07825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN86678.1"
                     /translation="MERAVDSALDQGAVEVVVVDNRSTDGTWDVVQKFAAAGVRVHRN
                     ERNLGLFGNFNRCLELATTRYLRLLAGDDALAPGCLAAEIELMGRHADVVMLSTRGLF
                     VDGQGAPLGEFAGDFPPGIYDGRRFSRAWLEYYVHYRRNTLNYPSGVLLRRSSLGGVR
                     FD"
BASE COUNT          561 a         1408 c         1375 g          650 t
ORIGIN      
        1 ggcggcgacc agcgcgagcc ttatctcgaa acggaccgcc cgcagccgct gcaggtctat
       61 ggcgcgaccc gtctcgcggg cgagaacctg gcgctggccg cggcgccgca gcgtgcggtg
      121 gtcatccgca cctgtggcct gtatggcacg gcgggctcgc gctcgaaggg cggcaacttc
      181 gtcgacgggc gggtggccga cggccgcgcc ggcaagcgca tcgagatggc gagcgagcag
      241 acggtcgcgc cgacctcgac gcacgacctt agccgggcgg tgcacgcact cctgcagcat
      301 ccgaaactgg cgccgggcct gtatcacctg gtcaacgaag gaagctgtac ctggtacgag
      361 ctcacgcgcg cgatcgtgga gacgctgcgc ctgccggccg aggtggtgcc ggtggaccgc
      421 ggcgggcgca gcggtgcgat gcgccgcccg ttgtactcgg tgctcgccaa cacccgtgcg
      481 cgagcgctgg gcatccgcct gcggccgtgg cgggaagcgc tggtcgatta cctgcgcgcc
      541 aagtacccgt cctgatggcc gacgtcggcg tcgtccatct ggtgcgtgag gcgaacggcc
      601 tggcgccgct cgagcggttc atcgcttcct accgcgagca gcccgcgggc cacgcgcacg
      661 agctgatcct ggtgtgcaag ggcttcccgg gacgcctgcc cgccgagtat gaacgcgcgc
      721 tcgaaggcgt cgcgtatcgc accctgcacg tggcggaccg cggcttcgac ctcgcgccgt
      781 acttcgccgc gacgcgccgt ttcgagcacc gctacttctg cttcctgaat tccttcagcc
      841 ggcccctcgc cgccggctgg ctcgagatcc tgcatcgctg cgcgagcgct ccaggagtcg
      901 gcctggtggg cgcgaccggc tcctatcaga gcttcgcgca gtcgagcgag gagcgtcgac
      961 gcctcggcac gcgcgcgcgg ctgcggcatg tggcgcgcgg cggcagcccc gccatggtgg
     1021 cgcagcgaac cggagcctgg gtgctgggtg gccttggcct gtggaagccg gcccggcatt
     1081 ttcccgcctt ccccaactac catatccgca ccaatgcctt catggcctcg aaggacgtgc
     1141 tgttgcgcat ccgcgtgcgg ccgatcctgt tcaagctgtc cgctttcctg ctcgagagcg
     1201 ggcgcagcgg cctcacgcgc caggtgatgg ccatgggact gcgcgcgctg gtggtcgatc
     1261 gcgccggccg cgccttcgag aaggaaagct ggcacttggc gaacacgttc cgccagtcgc
     1321 gccaggagga cctgctggtc gaggacaacc agaccgtcgc gtatgagcac gcgcagcctg
     1381 aggaacgggg gaatctgtcc agaatcgcct ggggcgaatt tgccaggccg gcctgaatga
     1441 tctgcagtgg ctgcggcggc agcatgcgcc cgtatctgcg cacgcgcgat ctcaaccggg
     1501 atgcgtcgga cgaggaattc cgctacgagc gctgcccgga ctgcgggctc gcctctctcg
     1561 cgaacgcgcc agccgacctc gggcgctatt acgccgccgg ttaccacgaa atccccgctt
     1621 cggccgaagg cctcgagcgc ggcgccgcgc acgactcgta caagatcgac ctggtgcggc
     1681 agttcgcgac gcgcggccgc ctggtggaaa tcggcccggg ctggggcgcg ttctgcctgc
     1741 tggccaggcg cgccggcttc gaggtggaag cgatcgagcg cgacccgcgc tgctgcgagt
     1801 tcctgcagaa ggagcttggc gtgcgcgcga tccgcagcga cgacccggcg acggcgctgg
     1861 cgcaggccgc caccgctgat gcgatcgtcc tctggcacgt ggtcgagcac ctcgccgatc
     1921 cgtgggccgt gctcgaagcc gctgcgcgca agctcgcgcc gggcggggtg ctgctgatcg
     1981 ccacgccgaa ccctgaatcg ttccagctcg ggctgttcgg ccgcttctgg acccacgtcg
     2041 atgcgccgcg ccacctccac ctgattccgg tgcgcctgct gcgcagccgg atggcgcgac
     2101 tcggcctgga cgccgagctc gccacgacca ccgacccggg cagcatcggc tggaacgact
     2161 tcggctggcg cttctcgctt gccaacctcg cgccgcatcg cgcgctcaag cgtccgctgc
     2221 gcctcgcggg ccgcgtcctc ggcaagctcg cggccttgat cgacaaccgc gaaggtcgcg
     2281 gcagcgcgta tacggcggtg ttccgcaagc gatgaagttt tcgatcctgc tgccgacgcg
     2341 tgagcggctc gagcttttgc gccatgcggt cgaatcggtg cgagcccagg acgatgccga
     2401 ctgggaagtc gtgatctcgg acaatgcgtc gtcgcaggac gtagcgggct acgtcgccgc
     2461 gctgggcgac gcgcgcgtgc gctactcgcg caccgaccag ctcctgccgg tgacgcagaa
     2521 ctggaaccgc gcgctcgagg cgagccgcgg cgagtacctg atcatgctcg gcgacgacga
     2581 cgcgctgctg ccgcgctgcc tgagccgcgc ccgggccctg ctcgcgcaat ggcgcgatcc
     2641 cgacctcgtg tatacgcagg ccctgcagta cgcctatccc ggcgtcgttc cgggggcggc
     2701 cgagggcttc gtgcaggtcg gctacaacgc attcctgacg caaggcgcaa cccggccctt
     2761 cgtcctcgcg ccggaaacgg cgcacaccat ggtgcgcgaa gcgatgcgtt tccgcatccg
     2821 ctacggcttc aatgcccagc atttcatttt ttcgcgccgc ctggtggacg ccctgcgcgc
     2881 gaaggggccg ttcttccagt cgccctaccc ggactactac gcggcgaacg ccatgctgct
     2941 cgccgcgcgc aacatcgtgg ccaatcccga gccgctggtg ctgatcggca tcagccccaa
     3001 gtccttcggc ttctaccatc acaaccggcg cgagggcgag ggcgtcgact tcctgcagaa
     3061 tttcccctcg ccggaaatgc gcgcgcgcct cgctcccacg tgggtgccgg gctcgaacat
     3121 gaacgactcc tggctgtgcg cgatggaaac cctggcgatg aactttccgg agcgtgcgct
     3181 aagcgtgaac taccggcgct accgtctgct gcaatatcag gcgacgctcc gcgagcacgg
     3241 cttgcgcgcg gccctgcgtc acgtgcgctg gtgggagccg ctgctgtacg cgcccgcggt
     3301 gctggctttc ggcttcgctt acctgctgcc gcgcggcctg cgctcgcggg tgcaggacaa
     3361 ggtcaaggcg atgttcagcg cctacccgcg cttcgatgcg cagcgccgca cggtgcctta
     3421 ccgggacatc ctcgaagccg cgcggggagt gtcccgctga gcgccgcctg caccgtcgcc
     3481 atcccggtct tcaaccagcg cgccttcatc gagcgcgccg tcgattcggc gctggaccag
     3541 ggggcggtcg aagtcgtggt ggtcgacaat cgctcgaccg acggcacctg ggacgtggtg
     3601 cagaaattcg ccgccgccgg cgtgcgcgtg caccggaacg agcgcaacct cggcctgttc
     3661 gggaatttca accgctgcct ggagctcgca acgacgcgct acctgcgcct gctcgccggc
     3721 gacgacgcgc tggcgccggg ctgcctggcg gccgagatcg agttgatggg gcggcacgcg
     3781 gacgtggtga tgctcagcac gcggggcttg ttcgtggacg ggcagggtgc gccgctcggc
     3841 gagttcgccg gtgatttccc gccgggcatt tacgacggtc gccgtttctc gcgcgcctgg
     3901 ctcgagtact acgtgcacta ccggcgcaat acgctcaact atccttcagg cgtgctgctg
     3961 cggcgctcga gcctgggcgg cgtgcgcttc gacg
//
LOCUS       DASRIE010000190         6879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1746791, whole genome shotgun sequence.
ACCESSION   DASRIE010000190 DASRIE010000000
VERSION     DASRIE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746791"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFB53_07830"
     CDS             <1..553
                     /locus_tag="VFB53_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN86679.1"
                     /translation="TARCRVIAATNRDLRRAVREGAFRADLYHRLSVFTLSVPPLREL
                     DQDKLLLLDHFRKNAFELDPAATERWLRYGFPGNVRELRNIVLRLSAKYPGQRISVAE
                     LEAELDVDSAEPGAGRDSIADQALRQLERGAPFDLDQTLKAWERGYIEAALRLTRGNV
                     TQAAKLLGINRTTLYSRMGSDEK"
     gene            572..2011
                     /locus_tag="VFB53_07835"
     CDS             572..2011
                     /locus_tag="VFB53_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN86680.1"
                     /translation="MALYLEHFGLTEPPFRITPHTDFFFEGADRGATLEALIYAVLHD
                     EGIVKVSGEVGSGKTMLCRVLMERLPQHVDTLYLATPSLASHEILHAIGDELELKLSP
                     ERRSVALRELQEHLISLYGAGRRVVILVDEAHVMPDDTLEQVRLLSNLESNRHKLLQI
                     VLFGQPELDETLAKPSLRQLRDRITHSFRMRPLAVPEVGKYLSFRMRAAGYRGPEVFT
                     PRAVRRVARASGGLTRRINILADKALLAAYTESTHAITGRHVRAAIADSEFAAPRKPR
                     LLAYVAAAAVAGLAIGAASQWIVRSGAPAAAPATALQAPAAPAHSAAPPSPPAPAAEA
                     APLPPEPKPEAQSAPETEPMLSPEQARRLAGYSAAGHRLLAERLTAGRELLERAGDEH
                     YALELFVTENTDPARMERFLLRARELVPLEELYVIPVAGGEDYRLWVLFGEFATREEA
                     VAAARRLPPRYQQAFRAAPRNFGELRAQL"
     gene            2101..3858
                     /gene="mshL"
                     /locus_tag="VFB53_07840"
     CDS             2101..3858
                     /gene="mshL"
                     /locus_tag="VFB53_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HZN86681.1"
                     /translation="MNTGNALALASVALTLTIGGCGQTPIKPSPTHIRSDEPRAEGSI
                     PPPVQLTPVLPQPKPTARPETYSVVVNNVRVQELLFALARDARLNVDIHSDIAGTVTL
                     NAIDQTLPQLLTRIARQVDMRWEIEGQNLIVMRDTPYLRMYRVDYVNLARDARSVQNL
                     STQVSGASQPGAAAAASQNNSTAVINSISANKFWDTLVANIKEILQETDKVLPAGAAS
                     AAGVPPVASTTAPVQPGGAPAPLAVAAPTFREAASVISNPETGILNIRATSRQHEKVQ
                     EFLDQVLTSAKRQVLIEATVAEVQLNNEYQRGIDWQRLRTNAANVGVPAFGLGNSGME
                     FRQSSANTPANITTNAFVFGGAIGSLNFNFALKLLESFGDVRVLSSPKISVLNNQTAV
                     LKVVDNLVFFTIQAQSTANQTTTVTTFTTTLNSVPVGFILSLVPQISDTDTVVLNLRP
                     TISRKIGDVADPNPNLATANVVSLIPVIQTREMESVLRVQSGQVAVLGGLMQDASSRV
                     EDTIPGINRIPVLGALFEQRRDTNQKTELVIFLRPVVIRDASVDGDYSSYRGLLPGAD
                     FLSKPNPSRPLRPSERAGD"
     gene            3865..5232
                     /locus_tag="VFB53_07845"
     CDS             3865..5232
                     /locus_tag="VFB53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86682.1"
                     /translation="MSLLLEALKKAEKAKEEAQRRAQGPHSATAASPATLEAAAAPEE
                     PAVRTRDDLPDIRRPVEAASAEAPPRAQPELALEEEQPRATPERPAPAAAPRAQPRRE
                     TETQASPRAAARNVFEAKFREPSPRLPFFITLGALGAFAIGTMIYFWMQLRPPAPLVN
                     LNPKPPAGELQVAAAASTAPAAFAPAPTAQPTGIPGLPAASVASAAPSSLARAAKPAP
                     QALERAPETPRLQPQLRVPAPATRGPVPAASETEFSARRLAPQVHPKVGSAYAAYQAG
                     DLAAARADYQDALRDEPANRDALLGLAALDVRAGRLEAAEGAYLRLLQADPRDAHAQA
                     ALIALRAGRVDPVAAESRVKVMLADNPGAHVLNFTLGNQFAQQGRWAEAQQEYFKAFA
                     AEPENADFAYNLAVSLDHLRQPQLALQYYQRAITLAKARGAGFDLAAAEKRAAQLPAA
                     DPLRR"
     gene            5234..>6879
                     /locus_tag="VFB53_07850"
     CDS             5234..>6879
                     /locus_tag="VFB53_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZN86683.1"
                     /translation="MNQPAPPQAKRHLGQILIDQGILTEDQLRIALLEQTKQHIPVGK
                     LLVQLGFVSEATLRDALSEKLGLQSVDLSRIIVDPAALKHLPREMARRNRMFPVALDR
                     QQRKFIVALADTNDIVALDQLRAHLKGDYEVELRLAGDSEIERAIEHYYGHEFSIDGI
                     LKEIETGEVDYTTIAEGDEYSQPVVRLISALLADSVEHGASDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRLKVMAKMNIAETRAPQDGRISATMSGRVVDFRVASLP
                     TSHGENLVLRILDRQKGIVPLDKLGLEAGQLELLKRMIARPEGIILVTGPTGSGKTTT
                     LYSIMNHINSDGINVMTLEDPVEYPMTLIRQTSVSEAAKLDFANGIRALMRQDPDVIL
                     VGEIRDHDTAEMAFRAAMTGHQVYSTLHTNSAVGAFPRLLDIGIVPDILAGNIIGILA
                     QRLVRRLCRTCRQSYDPDVKERRLLGLSPTEPAALFRAVGCEKCQYQGYSGRQSILEL
                     LKIDAGIDELVARRATAREILTAARASGFRTLADDGVRLVR"
BASE COUNT         1118 a         2591 c         2253 g          917 t
ORIGIN      
        1 caccgcgcgc tgccgcgtca tcgccgccac caaccgcgac ctgcgccgcg cggtgcgcga
       61 aggcgccttc cgcgccgacc tgtaccaccg cctgtcggta tttaccctaa gcgtgccgcc
      121 gctgcgcgaa ctggaccagg acaagctgct gctgctggat catttccgca agaacgcttt
      181 cgagctggac ccagccgcga ccgagcgctg gctgcgctac ggctttcccg gcaacgtgcg
      241 cgagctgcgc aacatcgtgc tgcggctgtc ggccaagtat ccggggcagc gcatctccgt
      301 cgcggagctc gaagcggagc tcgacgtcga cagcgccgag cccggcgcgg gcagggactc
      361 gatcgccgac caggcgctgc gccagctcga gcgcggcgcg ccgttcgacc tcgaccagac
      421 gctcaaggcc tgggagcgcg gctacatcga ggcggcgctg cgtctcacgc gcggcaacgt
      481 cacccaggcg gcgaagctcc tgggaatcaa ccgcaccacg ctctactccc gcatgggaag
      541 cgacgagaaa tgagcgagga ggcggcgtag cgtggccctc tacctcgagc acttcggcct
      601 caccgagccg ccgttccgca tcacgccgca caccgacttc ttcttcgagg gcgccgaccg
      661 cggcgcgacg ctcgaggccc tgatctacgc ggtgctacac gacgagggca tcgtcaaggt
      721 gtcgggcgag gtcggcagcg gcaagaccat gctgtgccgg gtgctgatgg agcggctgcc
      781 gcagcacgtc gacaccctct acctcgccac gccgtcgctc gcgagccacg agatcctgca
      841 cgcgatcggc gacgagctcg agctcaagct ctcgcccgag cggcgcagcg tcgccctgcg
      901 cgagctgcag gagcatttga tcagcctgta cggcgccggg cggcgcgtcg tcatcctggt
      961 cgacgaggcg cacgtcatgc ccgacgacac gctggagcag gtgcggctgc tctccaacct
     1021 cgagtcgaac cgccacaagc tgctgcagat cgtgctcttc ggccagcccg agctcgacga
     1081 gaccctcgcc aagccgagcc tgcggcagct gcgcgatcgc atcacccatt ccttccgcat
     1141 gcgcccgctc gccgtgcccg aggtcgggaa gtacctgtcg ttccgcatgc gagcggcggg
     1201 ctatcgcggc cccgaggtgt tcacgccgcg cgcggtgcgc cgcgtggcgc gcgcctccgg
     1261 aggactgacg cggcgcatca acatcctcgc cgacaaggcg ctgctcgcgg cctacaccga
     1321 gagcacgcac gccatcaccg gtcgccacgt ccgcgcggcg atcgccgatt cggagttcgc
     1381 ggcgccgcgc aagccgcggc ttctcgccta tgtcgcggcg gcggccgtgg ccggacttgc
     1441 gatcggcgcc gcctcgcaat ggatcgtgag gtcgggcgcg ccggcagcgg ctccggccac
     1501 cgcgctgcaa gcgcccgcgg caccggcgca cagcgcggcg ccgccgagcc cgcccgcccc
     1561 ggcggccgaa gcggcgccgc tcccacccga gccgaaaccc gaagcccagt ccgcgccgga
     1621 gaccgagccg atgctttccc ccgagcaggc gcgccgcctc gcggggtatt ccgccgccgg
     1681 ccatcgtctg ctcgccgagc gactgacggc cggacgcgag ctgctcgagc gcgccggcga
     1741 cgagcattac gcccttgagc tcttcgtcac cgagaacacc gaccccgcgc gcatggagcg
     1801 ctttctcctg cgcgcgcgag agcttgtgcc gctcgaggag ctctacgtga tccccgtcgc
     1861 gggcggcgag gactaccgcc tctgggtgct gttcggcgag ttcgcgacgc gcgaagaagc
     1921 cgtggccgcc gcgcggcgcc tgccgccgcg ataccagcag gcattccgcg ccgcgccgcg
     1981 caacttcggc gagttgcggg cgcagttgta aaactgggaa aaggcctgcg cgacaggcgt
     2041 ttaccgcatc ttcttcattc gtgatcgtgt tatccttgcc gcgggaggga gtgcccggca
     2101 atgaacacgg ggaacgcgct cgcgctggcg agcgttgcgc ttactttgac catcggcggt
     2161 tgcggccaga cgccgatcaa gccgtcgccc acgcacatcc gttccgacga accgcgcgcc
     2221 gagggatcga tcccgccgcc ggtgcagctc acgccggtgc tgccgcagcc caagcccacc
     2281 gcccggccgg agacctacag cgtggtggtg aacaacgtgc gcgtgcagga gctgctgttt
     2341 gcgctggcac gcgacgcccg cctgaacgtc gacatccatt ccgacatcgc gggcaccgtg
     2401 acgctgaacg ccatcgacca gacgctgccg cagctcctta cgcgcatcgc gcgccaggtc
     2461 gacatgcgct gggagatcga gggccagaac ctcatcgtga tgcgcgatac gccctacctg
     2521 cgcatgtacc gggtcgatta cgtgaatctg gcgcgcgacg cgcgcagcgt gcagaacctc
     2581 tcgacgcagg tgagcggcgc ctcgcagccc ggcgcggcgg cggccgcctc gcagaacaac
     2641 tccaccgcgg tgatcaactc gatctcggcg aacaagttct gggacacgct ggtcgccaac
     2701 atcaaggaaa tcctgcagga gaccgacaag gtgctgccgg cgggcgcggc ttcggccgcc
     2761 ggagtgccgc cggtggcctc caccaccgcc ccggtgcagc ccggcggggc acccgcgccg
     2821 ctcgccgtgg cggcgccgac gttccgcgaa gccgcctcgg tcatctccaa ccccgaaacc
     2881 ggcatcctca acatccgcgc gacctcgcgc cagcacgaga aagtgcagga attcctcgac
     2941 caggtgctca ccagcgcgaa gcgccaggtg ctgatcgagg cgaccgtggc cgaggtgcag
     3001 ctcaacaacg agtaccagcg cggcatcgac tggcaacgcc tgcgcacgaa cgcggcgaac
     3061 gtgggcgtgc ccgccttcgg cctcggcaac agcggcatgg agttccggca gtcctcggcc
     3121 aacacgccgg ccaacatcac gaccaacgcc ttcgtgttcg gcggcgcgat cggcagcctg
     3181 aacttcaatt tcgcactcaa gctgctcgag tcgttcggcg acgtgcgcgt gctctccagc
     3241 ccgaagatca gcgtgctcaa caaccagacc gcggtgctga aggtggttga caacctggtg
     3301 ttcttcacca tccaggcgca gagcaccgcg aaccagacca ccaccgtgac gaccttcacc
     3361 accacgctga actccgtgcc cgtcggcttc atcctcagcc tggtgccgca aatcagcgac
     3421 accgacaccg tagtactgaa cctgcggccc accatctcgc gcaagatcgg cgacgtggcg
     3481 gatccaaacc ccaacctcgc cactgccaac gtcgtcagcc tgatcccggt gatccagacg
     3541 cgcgagatgg aatcggtgct gcgggtgcag agcggccagg tcgcggtgct gggcggcctg
     3601 atgcaggacg cctcgagccg tgtcgaggac acgatcccgg gtatcaaccg catcccggtg
     3661 ctcggcgcgc tgttcgagca gcgccgggat acgaaccaga agaccgagct cgtcatcttc
     3721 ttgaggccgg tggtgattcg cgacgcgagc gtcgacggcg actactccag ctaccgcggc
     3781 cttctgccgg gcgccgactt cctgtccaag cccaacccga gccggccgct gcgcccctcc
     3841 gaacgcgccg gcgattgagc tgacatgagc ctgctgctag aggcgctgaa gaaagccgag
     3901 aaggcgaaag aggaagcgca acgccgcgcg cagggcccgc acagcgccac cgcggcgtcg
     3961 ccggcaacgc tggaggccgc ggccgcgccc gaggagccgg cggtgcgcac ccgcgacgac
     4021 ctgcccgaca tccgccggcc cgtcgaggcg gcgagcgccg aggcgccgcc gcgcgcccag
     4081 cccgagcttg ccctcgagga agagcagccg cgcgccacgc ccgagcggcc ggcgcccgcc
     4141 gccgcgcctc gcgcccagcc gcgccgcgaa accgagaccc aggccagccc gcgcgcagcc
     4201 gcccgcaacg tgttcgaggc gaagttcagg gagcccagtc cccggcttcc cttcttcatc
     4261 acgctcggcg cgctcggcgc gttcgccata ggcacgatga tttatttctg gatgcagctg
     4321 cgcccgccgg cgccgctcgt caacctcaac ccgaaaccac cggccggcga gctgcaggtc
     4381 gccgccgccg cgagcaccgc gcccgccgca ttcgcccccg cgccgaccgc gcagccgacc
     4441 gggatcccgg ggctgccggc ggcctctgtg gcaagcgcgg ctcccagctc gctggcccgg
     4501 gcggcgaagc ccgcgcccca ggcgctcgag cgcgcccccg agacgccgcg cctccagccg
     4561 cagctgcgcg tgccggcgcc agccacgcgc ggtcccgtcc ccgcggcgtc cgaaaccgag
     4621 ttcagcgccc ggcgcctcgc gccgcaagtg cacccgaagg tcggatcggc ctacgcggcc
     4681 taccaggccg gcgacctcgc cgcggcgcgc gccgattacc aggacgcgct gcgcgacgag
     4741 cccgcgaatc gcgacgcgct tctcggcctc gcggcgctcg acgtgcgcgc cggccgcctc
     4801 gaggcggcgg agggcgcgta cctgcgcctg ctgcaggccg acccgcgcga tgcgcatgcg
     4861 caggccgcgc tgatcgcgct gcgcgcgggc cgcgtcgacc cggtcgcggc cgaaagccgc
     4921 gtcaaggtga tgctcgccga caaccccggc gcgcacgtgc tcaacttcac cctgggcaac
     4981 cagttcgccc agcagggccg ctgggccgag gcgcagcagg aatacttcaa ggctttcgcc
     5041 gccgagcccg aaaacgcgga cttcgcttac aacctcgcgg tcagcctgga ccacctgcgc
     5101 cagccgcagc tcgcgctgca gtattaccag cgcgcgatca ccctcgccaa ggcgcgcggc
     5161 gccggcttcg acctcgcggc ggccgaaaag cgcgcggcgc agcttcccgc cgccgacccg
     5221 ctgcgccgct gacatgaacc agcccgcgcc accgcaggcg aagcgccacc tcggccagat
     5281 cctgatcgac cagggcatcc tgaccgagga tcagctgcga atcgcgctcc tcgagcagac
     5341 caagcagcac atcccggtcg gcaagctgct ggtgcagctc ggcttcgtgt ccgaggccac
     5401 cctgcgcgac gcgctctcgg agaagctcgg cctgcagtcg gtcgacctgt cgcgcatcat
     5461 cgtcgacccg gcggcgctca agcacctgcc gcgcgagatg gcgcgccgca accgcatgtt
     5521 cccggtggcg ctcgaccgcc agcagcgcaa gttcatcgtc gcgctcgccg acaccaacga
     5581 catcgtcgcg ctcgaccagc tgcgcgcgca cctcaagggc gactacgagg tcgagctgcg
     5641 ccttgccggc gactcggaga tcgagcgcgc catcgagcac tactacgggc acgagttctc
     5701 gatcgacggg atcctgaagg agatcgagac cggcgaagtg gactacacca ccatcgccga
     5761 gggcgacgag tactcgcagc cggtggtgcg gctgatctcc gcgctgctcg ccgactcggt
     5821 cgagcacggc gcctccgaca tccacttcga gccggagcag aacttcctgc gcatccgcta
     5881 ccggatcgac ggcgtgctgc gccagatccg ctcgttgcac aagacctact ggccggcgat
     5941 ggcggtgcgc ctgaaggtga tggcgaaaat gaacatcgcc gagacccgcg cgccgcagga
     6001 tggccgcatc tcggcgacga tgtcgggccg cgtcgtcgac ttccgcgtcg ccagcctgcc
     6061 gaccagccac ggcgagaacc tggtgctgcg gatactggac cgccagaagg gcatcgtgcc
     6121 gctcgacaag ctcggcctgg aggcggggca gctcgagctc ctgaagcgca tgatcgcgcg
     6181 cccggagggg atcatcctcg tgaccgggcc gaccggcagc ggcaagacca ccaccctgta
     6241 ctcgatcatg aaccacatca actccgacgg catcaacgtg atgacgctgg aggatcccgt
     6301 cgagtacccg atgacgctga tccggcagac ctcggtctcc gaggcggcga agctcgactt
     6361 cgccaacggc atccgcgcgc tcatgcggca ggaccccgac gtcatcctgg tgggcgagat
     6421 ccgcgaccac gacaccgccg agatggcgtt ccgcgccgcg atgacgggcc accaggtgta
     6481 ctccacgctg cataccaatt cggcggtcgg cgccttcccg cgcctgctcg acatcggcat
     6541 cgtccccgac atcctcgccg gcaacatcat cggcatcctg gcgcagcgcc tggtgcgccg
     6601 gctgtgccgc acctgccggc agtcctacga cccggacgtg aaagagcgcc ggctgctcgg
     6661 gctctcgccc accgaaccag cggcgctgtt ccgcgcggtg ggctgcgaga agtgccagta
     6721 ccagggctat agcgggcggc agagcatcct cgagctgctg aagatcgacg ccggcatcga
     6781 cgagctggtc gcgcggcgcg cgaccgcgcg cgagatcctc accgcggcgc gagcgagcgg
     6841 cttcaggacg cttgccgacg acggcgtgcg gctggtgcg
//
LOCUS       DASRIE010000191         8469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_427543, whole genome shotgun sequence.
ACCESSION   DASRIE010000191 DASRIE010000000
VERSION     DASRIE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427543"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFB53_07855"
     CDS             <1..513
                     /locus_tag="VFB53_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN86684.1"
                     /translation="STDEELHRYWAENLAQADALIFGRVTYEMMEAAWRPSATGVTPD
                     WMADWMNPFARTIDAAKKYVVSSTLERVDWNAELVRGDLEKAVRQLKGEPGKGLLTGG
                     VKLALALAELGLIDEYEFVVHPRLAGHGPALFAGLSKHVELKLVGRQELNSGAVAMRY
                     EPRRPPRRHS"
     gene            548..937
                     /locus_tag="VFB53_07860"
     CDS             548..937
                     /locus_tag="VFB53_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN86685.1"
                     /translation="MSRVSVRYIIEDVPAAVRFYTTLLGFAVEQDASPAFAAVVRDGV
                     RLLLSGDGSSGKRPLPDGRRQLAGGWNRVHLEVADLAAEVARLREAGVTFRMSDIVSG
                     PGGAQIILDDPSGNPVELFQPRRNPQK"
     gene            978..1640
                     /locus_tag="VFB53_07865"
     CDS             978..1640
                     /locus_tag="VFB53_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86686.1"
                     /translation="MLLQFDIVEEAIAEHDDWHTHEHLPERLAIPGFIRGTRWVALQG
                     RPRYFVMYEVEQLAVLTSQPYLERLNNPSPWTTKIMPSYRGMNRGLCSVAGSFGFGIG
                     HAGLLIRFKARTGAEGSARNRLLQDILPGLPSKPGIGSAHLFEGSLAAPMTNEQRIRG
                     ADAGVDSALLVTGYSQDALAALAQAELGNARLEAHGASGIAGATYRLDYSLAAREVRA
                     AP"
     gene            1710..1997
                     /locus_tag="VFB53_07870"
     CDS             1710..1997
                     /locus_tag="VFB53_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZN86687.1"
                     /translation="MSSVGYHEPVEELSDETRDMHRAIVSLMEELEAIDWYNQRADAC
                     KNPELKAILGHNRDEEKEHAAMVLEWIRRKDPVLSRELKDYLFTTKDIAHK"
     gene            2005..2262
                     /locus_tag="VFB53_07875"
     CDS             2005..2262
                     /locus_tag="VFB53_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86688.1"
                     /translation="MSEDQQREALEILREIRDRQREAVELLKAQRALVEEQLQKSRAS
                     VQESIGLQRTALARQRQVTLIAVPGIIACLAAIAYLVIRYF"
     gene            2279..2611
                     /locus_tag="VFB53_07880"
     CDS             2279..2611
                     /locus_tag="VFB53_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HZN86689.1"
                     /translation="METYFKPQHLADFPRVSDASPELGKKFFDYYGAVFAEGALTARE
                     KSLIALAVAHTVQCPYCIDAYTQDCQQKGVDLEQMTEAVHVAVAIRGGSSLVHGMQMR
                     EQAKKAGM"
     gene            2633..3700
                     /gene="arsS"
                     /locus_tag="VFB53_07885"
     CDS             2633..3700
                     /gene="arsS"
                     /locus_tag="VFB53_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255296106.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HZN86690.1"
                     /translation="MEELAADLAADRASPRAAQSLRRRAAPLAAPAEQLRLLGSLPLR
                     AEFGALAEGLAPAQLEIFQVNLGKLCNMTCRHCHVDAGPDRSDAMMDDATVDLVIQSI
                     RASGARTVDLTGGAPELHPRFKDLVQAARGAGKHVMDRCNLTVLLLPRNAGLVDWLAE
                     RQVEVVASLPHWRRPNTDAQRGDGVFERSVRALRILNAAGYGKGDPRRRLTLVSNPAG
                     AFLGAAQAAAEREWREGLRKGFGISFDRLFVLNNMPISRFLEWLQASGNLEAYMERLV
                     NAYNPGAIAGLMCRNTLSVGWDGRLYDCDFNQMLELSPAASAHIRDFDEAAWRRRDIV
                     TARHCFGCTAGAGSSCGGQTS"
     gene            complement(3702..4430)
                     /gene="dnaQ"
                     /locus_tag="VFB53_07890"
     CDS             complement(3702..4430)
                     /gene="dnaQ"
                     /locus_tag="VFB53_07890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZN86691.1"
                     /translation="MRWPTWGWTVSVPRQILLDTETTGLNAKLGDRVVEIGCIELLSR
                     RVTDNHFHRYINPERKSEEAAARVHGIADEFLAAQPKFADIAKEFVDYVRGTELIIHN
                     AGFDVEFLDQELARVGLGKLADHVAGIVDTLALARELHPGKKNSLDALCERYAVDNAH
                     RTLHGALLDARLLAEVYLAMTRGQDSLMMDQETPAEQAATAASIDASRLAVLRATPAE
                     LQAHEEYLERMAKEAGGKVLWRKP"
     gene            complement(4390..4833)
                     /gene="rnhA"
                     /locus_tag="VFB53_07895"
     CDS             complement(4390..4833)
                     /gene="rnhA"
                     /locus_tag="VFB53_07895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZN86692.1"
                     /translation="MTDGVVEVYADGACRGNPGPGGWGALLVEGGKERELHGGEPATT
                     NNRMELTAVIRALDALPRGSRVRLFTDSQYVQKGMTEWLAAWKRRGWRTADRQPVKNE
                     DLWRALEEAARGHQVEWHWVKGHAGHPGNERADALANLGVDSVRS"
     gene            complement(4826..5620)
                     /locus_tag="VFB53_07900"
     CDS             complement(4826..5620)
                     /locus_tag="VFB53_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN86693.1"
                     /translation="MDRQSFVSVSEAAAKYNGERASFSEWCTTPLGRHVLGWELAQFD
                     NAVEDVFGFRAAQVGLPEFDFLRQNRISYRFTLALEAGAALAADPLQLPLASQSLDLM
                     VLPHALESHLDAHHVLREAERVLRPEGQLVISGFNTASLWGLRRKLARRGSGSPWDAR
                     VVGLLKLKEWLQLLGFELNGGRFGCYAPPFASALWLERFAFMEQAGARWWPILGGVYV
                     VRAVKRVHGMRVITPAWRKERARRRAVAALPQKNGHPGHAARRRHD"
     gene            5619..6383
                     /gene="gloB"
                     /locus_tag="VFB53_07905"
     CDS             5619..6383
                     /gene="gloB"
                     /locus_tag="VFB53_07905"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZN86694.1"
                     /translation="MIDIVPLQAFKDNYVWTLRNATHAAVVDPGEARPVLDYLARERL
                     TLAAILATHHHPDHVGGIPELLAAHRVPVYGPKGEPIATLTHPVAEGDEVAIPALGVT
                     LSVLDIPGHTRAHIAYYGVESLFCGDTLFACGCGRLFEGTPEQMMASLSKLAALPDAT
                     RVYCGHEYTLANIQFAQEVEPRNAALALRLARDRKLREAGRPTLPSTLGEERATNPFL
                     RWREPAVIESANKYLGARVSDPVRVFAAIRDWKNRF"
     gene            6401..7822
                     /locus_tag="VFB53_07910"
     CDS             6401..7822
                     /locus_tag="VFB53_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZN86695.1"
                     /translation="MQWLSARTGSIFAIAAGLALAGCSTLPEAPAEAEPAVIAPPRAA
                     APETIAIPEVRLLDATRLPPFEGEKRELDAPRSVAKLDRTQPPADLWQRLRNGFAMQD
                     IESPQVAERTAWYVANQDYLKRVFDRSRLYLYHIVEELEKRGMPMEIALLPIVESAFN
                     PMAYSRAHASGLWQFIPGTGKRYELKQNWWYDGRRDIVASTSAALDYLKDVYDMHGDW
                     HLALASYNWGENAVARAIAKNRKEGKPTDYMSLPMPRETRGYVPKLQALKNLIANPAA
                     HGITLQAIPNEPYFAVITKTRDIDVLLAAKLAEIPVDEFIALNPGFSRPLIRSGGAAR
                     IVLPADKVDVFHENLEEYEDRSLVSWKTYKPQRGDSLEGIARKHGLTLGQLREVNGIH
                     PRSARIPELLVVPIAGGAESFARLPIMYAPPIPKGGGRVHVVRRGETVWSIAQRYRMS
                     VAELKRWNRIGRYLAVGQRLVIR"
     gene            complement(7819..>8469)
                     /locus_tag="VFB53_07915"
     CDS             complement(7819..>8469)
                     /locus_tag="VFB53_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN86696.1"
                     /translation="ESGSGKTTVGKALLQLVRPTGGSVRHGGEELTRLARGALKAHRA
                     RMQIIFQDPYASLNPRMRVADILAEGMRSLGVGRDEADRTGRIDALLGQVGLPAQAKG
                     RYPHEFSGGQRQRIAIARALAVEPRLIVCDEPTSALDVSVQAQILNLLAELQARLGLA
                     YLFITHNLAVVEHLAHEVAVMRAGRIVEHGTAAQVLRAPRHAYTRELLAAVPKLQG"
BASE COUNT         1307 a         3049 c         2888 g         1225 t
ORIGIN      
        1 tcgacggacg aagagttgca tcgttactgg gccgagaacc tcgcgcaggc cgatgcacta
       61 atcttcggcc gggtaaccta cgaaatgatg gaggccgcct ggcggccgtc ggcgactgga
      121 gtgacgcccg attggatggc cgactggatg aaccctttcg cacggacgat cgacgcggca
      181 aagaagtacg tcgtgtcgag caccctcgag cgggtcgact ggaacgcgga gctcgtgcgc
      241 ggggatctgg agaaggccgt ccggcagctc aagggggagc ctggcaaggg actgcttacg
      301 ggaggcgtga agctcgcgct ggcgttggcg gagctgggat tgatcgatga gtacgagttc
      361 gtggtgcacc ccaggctggc gggccacggg ccggcgttgt tcgcggggct gtcgaagcat
      421 gtcgagctga agctcgtagg ccggcaggag ttgaattccg gtgcagtggc gatgcggtat
      481 gagccgagac ggcctccgcg ccgccattca tgaaatgatg cgagccattg gcgtgaggta
      541 aatagcgatg tccagggtca gcgtgcgcta catcatcgaa gacgtcccgg cggcggtgcg
      601 cttctatacc accctcctcg gcttcgcagt ggagcaggac gcctcgccgg ccttcgcggc
      661 ggtcgtgcgg gacggcgtgc gcctcctgct cagtggtgac ggcagttcgg gcaagcgccc
      721 cctgccggac ggccgccggc agcttgcggg cggctggaac cgcgtgcacc tggaggtggc
      781 cgatctcgcc gccgaggttg cgcgcctgcg cgaggcgggc gtcaccttcc gcatgagcga
      841 catcgtgtcc ggccccggcg gcgcgcagat catcctcgac gatccttcgg gcaacccggt
      901 ggagctgttc cagccccggc gcaatccgca aaagtgacca gggagtagcg cagctgccgc
      961 tgttcggaaa cgccgccatg ctcctccagt tcgacatcgt cgaggaggcg atcgcggagc
     1021 acgacgactg gcacacgcac gagcacctgc cggagcggct cgccattccg ggcttcatcc
     1081 gcggcacgcg ctgggtggcg ctgcagggtc ggccgaggta cttcgtgatg tacgaagtcg
     1141 agcagctcgc cgtcctcacc tcgcagccct atctcgagcg cctgaacaac ccgagcccct
     1201 ggaccacgaa aatcatgccc tcctaccggg gcatgaaccg gggcctgtgc tcggtggcgg
     1261 gcagtttcgg cttcggcatc ggccacgccg gcctgctgat ccgcttcaag gcaaggacgg
     1321 gggcggaagg atcggcgcga aaccggctgc tgcaggacat ccttcccggc ctgccttcga
     1381 agccggggat cggcagcgcc caccttttcg aggggagcct ggcggcgccg atgaccaacg
     1441 agcagcgcat ccgcggcgcc gacgccggcg tggactcggc gctcctggtc accgggtaca
     1501 gccaggacgc cctggcggct ctcgcgcaag ccgagcttgg caacgcccgg ctcgaagcgc
     1561 atggcgcgtc cggcatcgcg ggcgccacgt accggctgga ctactcgctc gccgctcgcg
     1621 aggtccgcgc cgcaccctga gcgcctcatc gccgccgggc attgcgtcat aattggcggc
     1681 ctgaccaacg ccatcccatg gagaactgaa tgtccagcgt cggctatcac gaaccggtgg
     1741 aggaactgtc cgacgaaacg cgcgacatgc accgcgccat cgtctccctg atggaggaac
     1801 tcgaagcgat cgactggtac aaccagcgcg ccgacgcctg caagaacccg gagctgaagg
     1861 cgatcctggg ccacaaccgc gacgaggaga aggagcacgc cgccatggtg ctggagtgga
     1921 tccgccgcaa ggacccggtg ctctccaggg aattgaagga ctacctgttc accacaaagg
     1981 acatcgcgca caagtgaggc tcgcatgagc gaagaccagc aacgcgaggc gctcgagatc
     2041 ctgcgcgaga tccgcgaccg ccagcgcgag gcggtggagc tgctcaaggc gcagcgcgcg
     2101 ctggtcgagg agcagctgca gaaatcgcgc gcctcggtgc aggaatcgat cggcctgcag
     2161 aggaccgcgc tcgcgcgcca gcggcaggtg acgctgatcg ccgtgcccgg gatcatcgcc
     2221 tgcctcgccg ccatcgccta tctcgtgatc cgctatttct gagcgcagaa gggttgccat
     2281 ggaaacctac ttcaagccgc agcacctcgc cgacttcccc agggtctccg acgccagccc
     2341 ggagctcggc aagaaattct tcgactacta cggcgccgtg ttcgccgagg gcgccctcac
     2401 cgcgcgcgag aagagcctca tcgcgctggc cgtcgcgcac accgtgcaat gcccctactg
     2461 catcgacgcc tacacccagg actgccagca gaaaggcgtg gacctggagc agatgaccga
     2521 ggcggtgcac gtggcggtgg ccatccgcgg cggctcgtcc ctcgtccacg gcatgcagat
     2581 gcgcgagcag gcgaagaagg cgggcatgta ggtgcgccag gccgtcatcc ggctggaaga
     2641 actcgcggcc gatctcgccg ccgaccgcgc ctccccgcgc gcggcgcaga gcctgcgccg
     2701 gcgcgccgcg ccgctcgccg cgccggccga gcagctgagg ctgctgggct ccctcccgct
     2761 gcgcgcggaa ttcggcgcgc tggccgaggg cctcgcgccc gcgcaactgg aaatcttcca
     2821 ggtgaacctg ggcaagctct gcaacatgac ctgcaggcac tgccacgtgg acgcgggccc
     2881 ggaccgcagc gacgccatga tggacgacgc caccgtggac ctggtcatcc agagcatcag
     2941 ggccagcggc gcccgcacgg tggacctcac cggcggcgcg cccgagctgc acccgcgctt
     3001 caaggacctc gtgcaggcgg cgcgcggcgc cggcaagcac gtcatggacc gctgcaacct
     3061 caccgtgctc ctcctgccgc gcaacgccgg cctggtggac tggctggccg agcgccaggt
     3121 ggaggtcgtc gcctcgctcc cccactggcg ccgccccaac accgacgcgc agcgcggcga
     3181 cggcgtgttc gagcgctcgg tgcgcgcgct gcgcatcctc aacgccgcgg gctacggcaa
     3241 gggcgacccg cggcgccgcc tcactctggt gtccaacccg gccggcgcct tcctcggcgc
     3301 cgcgcaggcc gcggcggagc gcgaatggcg cgaaggactg cgcaagggat tcggcatcag
     3361 cttcgatcgc ctcttcgtgc tgaacaacat gccgatctcg cgcttcctcg aatggctgca
     3421 ggcctcgggc aacctcgagg cctacatgga gcgcctggtg aacgcctaca acccgggcgc
     3481 gatcgcgggc ctgatgtgcc gcaacacgct ctcggtgggc tgggacgggc ggctgtacga
     3541 ctgcgacttc aaccagatgc tggagctctc gcccgccgcg agcgcgcaca tccgggattt
     3601 cgacgaggcg gcgtggcgcc ggcgggacat cgtcaccgcg cgccactgct tcggctgcac
     3661 cgccggcgcc ggcagctcct gcggcgggca gacgtcctag ctcagggctt gcgccacagc
     3721 accttgccgc cggcctcctt cgccatgcgc tcgaggtatt cctcgtgcgc ctgcagctcg
     3781 gcgggcgtgg cgcgcagcac cgccagcctg gaagcgtcga tcgaggccgc cgtcgccgcc
     3841 tgctccgcgg gcgtctcctg gtccatcatc aggctgtcct ggccgcgcgt catcgccagg
     3901 tagacctccg ccagcaggcg cgcatcgagc agcgcgccgt gcagcgtgcg gtgcgcgttg
     3961 tccaccgcgt agcgctcgca cagcgcgtcg agcgaattct tcttgcccgg atgcagctcg
     4021 cgcgccagcg cgagcgtgtc gacgatgccg gcgacgtggt ccgcgagctt gcccaggccc
     4081 acgcgcgcga gctcctggtc gaggaattcc acgtcgaagc ccgcgttgtg gatgatcagc
     4141 tccgtgccgc gcacgtagtc cacgaactcc ttcgcgatgt cggcgaattt cggctgcgcg
     4201 gcgaggaatt cgtcggcgat gccgtgcacg cgcgccgcgg cctcctcgct cttcctctcc
     4261 gggttgatgt agcggtggaa atggttgtcg gtgacgcggc ggctgagcag ctcgatgcag
     4321 ccgatctcca ccacgcggtc gcccagcttg gcgttgaggc cggtggtctc cgtgtccagc
     4381 aggatctgcc taggaacgga cactgtccac ccccaggttg gccagcgcat ccgcgcgctc
     4441 gttgccgggg tgcccggcgt ggcccttcac ccagtgccac tccacctggt ggccgcgcgc
     4501 ggcctcctcg agcgcgcgcc acaggtcctc gttcttcacc ggctgcctgt ccgcggtgcg
     4561 ccagccgcgc cgcttccagg ccgccagcca ctcggtcatg cccttctgca cgtactgcga
     4621 atcggtgaac agccgcacgc ggctgccgcg cggcagcgca tcgagcgcgc ggatcaccgc
     4681 ggtcagctcc atgcggttgt tggtggtggc gggctcgccg ccgtgcagct cgcgttcctt
     4741 gccgccctcg acgagcagcg cgccccagcc cccgggcccg gggttgccgc ggcaggcgcc
     4801 gtcggcgtac acctccacca cgccgtcagt catgccgccg gcgcgcggcg tggcccgggt
     4861 ggccgttctt ctgcggcagc gccgccaccg cgcggcgccg cgcccgctcc ttgcgccagg
     4921 ccggcgtgat cacgcgcatg ccgtgcacgc gcttcaccgc gcgcaccacg tacaccccgc
     4981 cgaggatcgg ccaccagcgc gcgccggcct gctccatgaa cgcgaagcgc tccagccaca
     5041 gcgcgctggc gaacggcggc gcgtagcagc cgaagcgccc gccgttcagc tcgaagccga
     5101 gcagctgcag ccattccttg agcttgagca ggccgaccac gcgcgcgtcc cagggcgagc
     5161 ccgagccgcg gcgcgccagc ttgcggcgca ggccccacag cgaggccgtg ttgaagcccg
     5221 agatcaccag ctggccctcg gggcgcagca cgcgctcggc ctcgcgcagc acgtggtgcg
     5281 cgtcgaggtg cgattccagc gcgtgcggca gcaccatcag gtccaggctc tgcgaggcga
     5341 gcggcagctg cagcgggtcg gcggcgagcg ccgcgcccgc ctccagcgcc agcgtgaagc
     5401 ggtaggagat gcggttctgg cgcaggaagt cgaattccgg cagcccgacc tgcgcggcgc
     5461 ggaagccgaa cacgtcctcc accgcgttgt cgaactgcgc cagctcccag ccgagcacgt
     5521 ggcgcccgag cggcgtggtg caccactccg agaaggacgc gcgctcgccg ttatacttgg
     5581 ccgcggcctc ggagaccgag acgaatgact ggcgatccat gatcgatatc gttccgctgc
     5641 aggctttcaa ggacaactac gtctggacgc tgcgcaatgc cacccatgcc gcggtggtgg
     5701 atcccggcga ggcgcgcccg gtgctggact acctcgcgcg cgagcgcctg accctggcgg
     5761 cgatcctcgc cacccaccac caccccgacc atgtcggcgg catcccggaa ctcctcgccg
     5821 cgcaccgggt gccggtgtac gggccgaagg gcgagcccat cgccaccctc acgcacccgg
     5881 tggccgaagg cgacgaagtc gccattcccg cgctgggcgt cacgctctcg gtgctcgaca
     5941 tccccggaca tacccgggcc catatcgcct attatggcgt ggaatcattg ttttgcggcg
     6001 acaccctgtt cgcctgcggc tgcgggcgcc tgttcgaagg caccccggag cagatgatgg
     6061 cttcgctgtc caagctcgcc gcgctgcccg atgcgaccag ggtctactgc gggcacgaat
     6121 acacgctcgc caacatccag ttcgcgcagg aagtggagcc gcgcaacgcc gcgctcgccc
     6181 tgcgcctcgc gcgcgaccgg aagctgcgcg aggcgggccg gcccaccctg cccagcacgc
     6241 tcggcgagga gcgcgccacc aaccccttcc tgcgctggcg cgagccggcg gtgatcgagt
     6301 cggccaacaa gtacctcggg gcacgggttt ccgacccggt ccgggtgttc gccgccatcc
     6361 gggactggaa gaaccgcttt taggctagta tccttcttcc gtgcaatggc ttagcgcaag
     6421 gactggaagt attttcgcga ttgccgcggg tcttgcgctc gccggctgct cgacgctgcc
     6481 ggaggcaccg gcggaggccg agccggcggt gatcgcgccg ccgcgggcgg cggcgcccga
     6541 gaccatcgcg attcccgagg tgcggctgct cgacgccacg cgcctgccgc cgttcgaagg
     6601 cgagaagcgc gagctcgatg cgccgcgcag cgtcgccaag ctcgaccgca cgcagccgcc
     6661 cgccgacctg tggcagcgcc tgcgcaacgg cttcgccatg caggacatcg aatcgccgca
     6721 ggtggccgag cgcaccgcct ggtacgtcgc caaccaggat tacctcaagc gcgtcttcga
     6781 ccgcagccgg ctgtacctct accacatcgt cgaggagctg gagaagcgcg gcatgccgat
     6841 ggagatcgcg ctgctgccga tcgtggagag cgccttcaac ccgatggcct actcgcgcgc
     6901 ccacgcctcc gggctgtggc agttcattcc cggcaccggc aagcgctacg agctgaagca
     6961 gaactggtgg tacgacggcc gccgcgacat cgtcgcctcg accagcgccg cgctcgacta
     7021 cctgaaggac gtgtacgaca tgcacggcga ctggcacttg gcgctcgcct cctacaactg
     7081 gggcgagaac gcggtggcgc gcgccatcgc gaagaaccgc aaggaaggca agccgaccga
     7141 ctacatgagc ctgccgatgc cgcgcgagac gcgcggctac gtgcccaagc tccaggcgct
     7201 gaagaacctg atcgccaacc cggcggcgca cggcatcacg ctgcaggcga tccccaacga
     7261 gccctacttc gccgtcatca ccaagacgcg cgacatcgac gtgctgctcg ccgccaagct
     7321 ggccgagatc ccggtggacg agttcatcgc gctcaacccc ggcttcagcc ggccgctgat
     7381 ccgcagcggc ggcgcggcgc gcatcgtgct gccggccgac aaggtggacg tgttccacga
     7441 gaacctggag gagtacgagg accggtcgct cgtttcctgg aagacctaca agccgcagcg
     7501 cggcgactcg ctggagggca tcgcgcgcaa gcacggcctt accctcggcc agctgcgcga
     7561 ggtgaacggc atccacccgc gctcggccag gatccccgag ctgctggtgg tgccgatcgc
     7621 gggcggcgcg gagagcttcg cgcgcctgcc gatcatgtac gcgccgccca tccccaaggg
     7681 cggcgggcgc gtgcacgtcg tgcggcgcgg cgagacggtc tggagcatcg cgcagcgcta
     7741 ccgcatgagc gtcgccgagc tcaagcgctg gaaccgcatc ggccgctacc tggccgtcgg
     7801 ccagcgcctg gtgatccgct agccctggag tttcggcacc gcggcgagca gctcgcgggt
     7861 gtaggcgtgc cgcggcgcgc gcagcacctg cgccgccgtg ccgtgctcga cgatgcgccc
     7921 ggcgcgcatc accgccacct cgtgcgccag gtgctccacc accgccaggt tgtgggtgat
     7981 gaacaggtag gcgaggccca gtcgcgcctg cagctcggcg agcaggttga ggatctgcgc
     8041 ctgcaccgac acgtcgagcg cgctggtggg ctcgtcgcac acgatgagcc gcggctccac
     8101 cgccagcgcg cgcgcgatgg cgatcctctg gcgctgcccg ccggagaatt cgtgcggata
     8161 gcggcccttg gcctgcgccg gcaggcccac ctgcccgagc agcgcgtcga tgcgcccggt
     8221 gcgatcggcc tcgtcccgcc ccacgccgag gctgcgcatg ccctcggcga ggatgtcggc
     8281 cacgcgcatg cgcgggttga gcgaggcgta agggtcctgg aagatgatct gcatgcgcgc
     8341 gcggtgcgcc ttgagcgccc cgcgcgcgag gcgcgtcagc tcctccccgc cgtgccgcac
     8401 gctgccgccc gtcgggcgca ccagctgcag cagcgccttg ccgacggtgg tcttgcccga
     8461 gcccgattc
//
LOCUS       DASRIE010000192         6487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1502794, whole genome shotgun sequence.
ACCESSION   DASRIE010000192 DASRIE010000000
VERSION     DASRIE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502794"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFB53_07920"
     CDS             complement(<1..545)
                     /locus_tag="VFB53_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZN86697.1"
                     /translation="MSTTVSRSAALLTTSRVRQFYALTKPRVVQLIVFCAVIGMLLAV
                     PGLPDWRTALAATVGIWLVASAAAAFNCLVEEKIDARMARTAWRPTAKGELTHAQTLT
                     FSAVLCGAGSALLVVFVNPLTMALTLATFVGYAVVYTVVLKPLTPQNIVIGGASGAMP
                     PVLGWAAMRAEVGPEALMLCL"
     gene            complement(557..1708)
                     /locus_tag="VFB53_07925"
     CDS             complement(557..1708)
                     /locus_tag="VFB53_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZN86698.1"
                     /translation="MAAPIDLAPLARMAALGAVLALGPLAWIWLRHRGGSPAARLQAL
                     TALTLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYGQVSPLGAHAEIQAAQEALPTGPV
                     THGKAWIEMVHRYLATGVGVLIVVLAAARWRESRRDPRAPSPGWAVATLAWVCVQGAF
                     GALTVTMKLYPAIVTLHLLGGIGLLALLAAQAQAPRVGSLPLPRGLRAGAWGVLAIAI
                     AQCALGGWVSANYAVLACSDFPTCQGAWWPAMDARQGFAVWRPLGVGPDGAAIPFAAL
                     TAIHFAHRLGALVALAALVLLAWRLAATRGARERRWAGALLGIGGWQLATGLGNVVLG
                     WPLAAAVSHTGGAAALVVVLTALLARAHRADAALPRPAPATVARRASPA"
     gene            complement(1722..2432)
                     /locus_tag="VFB53_07930"
     CDS             complement(1722..2432)
                     /locus_tag="VFB53_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86699.1"
                     /translation="MSGSNSSGRGGSVATDAPLAFTVHSLPSPALADDEARRTASGRW
                     KMLGILAVCAAPVVASYFTYFVVRPQARTNYGELVTPARAAPDGLPLATLAGEAVAPS
                     SLQGQWLLVVVGGAACERTCESQLLLQRQLREALGPEKGRVDKVWLVSDAAAPRAEVL
                     QAVAHGDPVTVLRVPAEALARWLEPAPGHALGDHLYVVDPMGRWMMREPASPDASRVK
                     RDLDRLLRASAGWDRAGR"
     gene            complement(2386..3111)
                     /locus_tag="VFB53_07935"
     CDS             complement(2386..3111)
                     /locus_tag="VFB53_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZN86700.1"
                     /translation="MTTFIRSHYGVLLAALAAVAASVSLGAWQLDRAAQKRALQEALD
                     TRAALAPLGAADLATTADAARSQHHRRVRLHGRWLDRFTVFLDNRQMNGRPGFFVVTP
                     LLPDGAAVAVVVQRGWMPRDPADRTRLRPVPAPAGPAVVEGRIAPPPSRLLELAPAAG
                     GVIRQNLDLAAFAGETGVPLAPLSVVQTSGPDDGLSRQWPAPAADVHKHYGYAFQWFA
                     LAALIAGLYVWFQLLRPRRQRRH"
     gene            3098..3322
                     /locus_tag="VFB53_07940"
     CDS             3098..3322
                     /locus_tag="VFB53_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZN86701.1"
                     /translation="MKVVIIVAFIAILGALAFAGVAMIRGREQEGKPKPKTMARALAW
                     RIGLSVALFLLILLAYRFGWIQPTGVPTGR"
     gene            complement(3362..4246)
                     /locus_tag="VFB53_07945"
     CDS             complement(3362..4246)
                     /locus_tag="VFB53_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZN86702.1"
                     /translation="MSAATPHGHTPYYFVPAPSRFPVMAAIGLFFVIFGAGQWVNGAS
                     WGKYSLLLGLLFWAFTMQGWFRQAISESEGGLYGDRVDVSFRWSMSWFIFSEVMFFGA
                     FFGALFWSRLYTIPVLGSLQNAILWPDFKAVWPSVQPGATASPAGIVEPFTTMGPWPI
                     PTINTALLLASGVTLTIAHHLLIAGRRGATVFWMWITVLLGVTFLSLQIYEYMHAYQD
                     LNLKLNSGIYGSTFFMLTGFHGFHVFVGMLMLLFITLRIMKGHFTPQRHFGFEGAAWY
                     WHFVDVVWLGLYVIVYWF"
     gene            complement(4323..4565)
                     /locus_tag="VFB53_07950"
     CDS             complement(4323..4565)
                     /locus_tag="VFB53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HZN86703.1"
                     /translation="MSPADNGGLKHAVRRKGSFLQTMRAVAWSFFGVRKSAEYEKDVS
                     QLNPVHVIVAGIVAAVLFIVVLVLLVNWVISSGVAS"
     gene            complement(4642..5277)
                     /locus_tag="VFB53_07955"
     CDS             complement(4642..5277)
                     /locus_tag="VFB53_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZN86704.1"
                     /translation="MNAPPRSTAPHDAGLRRDNRRMVGKLVVVTAAMFGFGYALVPVY
                     RSICEALGVNVLSLAERTRPGAAAAPAAGTQVDRSRKVTVEFDANARGVWDFKPEQRS
                     VEVHPGEMTTVMYEFRNLQPRAMAAQAIPSYAPKQASPYFDKLECFCFNEYTLQPGEV
                     RRWPVVFVIDPRLPKDVSTITLSYTFFEVGGRVPPAPAARAANPPAQEARS"
     gene            complement(5295..5402)
                     /locus_tag="VFB53_07960"
     CDS             complement(5295..5402)
                     /locus_tag="VFB53_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HZN86705.1"
                     /translation="MTDRRKQNQRLGWILASVVAALFVGFVAKAALLGI"
     gene            complement(5399..>6487)
                     /locus_tag="VFB53_07965"
     CDS             complement(5399..>6487)
                     /locus_tag="VFB53_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN86706.1"
                     /translation="MRAPGMTLMKMPLFCWTWLITAYLLIAVMPVLAGAITMTLTDRH
                     FGTSFFNPAGGGDPVMYQHIFWFFGHPEVYIMILPAFGIISAIVPAFARKRLFGYTSM
                     VYATASIAILSFIVWAHHMFTTGMPVTGQLFFMYATMLIAVPTGVKIFNWVATMWRGS
                     MTFETPMLFAVGFIFVFTIGGFTGLILAMAPIDIQLQDTYYVVAHFHYVLVAGSLFAL
                     FGGVYYWGPKWTGVMYDESRGKIHFWGSIIFFNVTFFPMHFLGLAGMPRRYADYPLQF
                     TDFNVVASVGALGFGLMQVYFFLMVVLPMMRGRGAPAPQQPWEGAEGLEWEIPSPAPY
                     HTFETPPRLNATATKVIGASATAAPHHA"
BASE COUNT         1053 a         2290 c         2346 g          798 t
ORIGIN      
        1 atgaggcaca gcatcagcgc ctcggggccg acctcggcgc gcatcgcggc ccagccgagc
       61 accggcggca tcgcgcccga ggcgccgccg atcacgatgt tctgcggcgt cagcggcttc
      121 agcacgacgg tgtagaccac cgcgtagccg acgaaggtcg cgagcgtcag cgccatcgtc
      181 agcggattga cgaagacgac cagcagcgcg ctgcccgcgc cgcacagcac cgctgagaac
      241 gtgagcgtct gcgcgtgcgt gagctcgccc ttggcggtgg ggcgccatgc cgtgcgcgcc
      301 atgcgcgcgt cgatcttctc ctcgaccagg cagttgaacg cggctgccgc ggaggccacc
      361 agccagatgc cgacggtggc cgccagcgcg gtgcgccagt ccggcaggcc gggcacggcg
      421 agcagcatgc cgatcaccgc gcagaagacg atgagctgca cgacccgcgg cttggtgagg
      481 gcgtagaact gccgcacgcg ggacgtcgtg agcaatgcgg cggagcggga gacggtggtc
      541 gacatggtcg cgaaggtcag gccggcgagg cgcgtcgggc caccgtggcc ggcgccgggc
      601 gcggcagggc ggcatccgcg cggtgtgcgc gcgcgagcag agcggtcagc acgacgacca
      661 gcgccgccgc gccgcccgtg tgcgacacgg ccgcggcgag cggccagccg agcacgacgt
      721 tgccgaggcc ggtggcgagt tgccatccgc cgatgccgag cagcgccccg gcccagcggc
      781 gctcccgcgc gccgcgcgtg gcggccaggc gccacgccag cagcacgagg gccgcgaggg
      841 ccaccagcgc gccgaggcga tgcgcgaagt ggatggcggt gagcgccgcg aacgggatgg
      901 ccgcgccgtc gggcccgacg ccgagcggcc gccacaccgc gaagccctgg cgggcatcca
      961 tcgcgggcca ccaggcaccc tggcaggtgg ggaagtcgga gcacgcgagc accgcgtagt
     1021 tcgcgctgac ccagccgccg agcgcgcatt gcgcgatcgc gatcgcgagc accccccagg
     1081 cccccgcgcg caggccgcgc ggcagcggca gcgagcccac ccgcggcgcc tgcgcctgcg
     1141 ccgccagcag cgcgagcagc ccgatgccgc cgagcaggtg cagcgtgacg atcgcggggt
     1201 acagcttcat cgtcaccgtc agcgcgccga acgcgccctg cacgcacacc caggccaggg
     1261 tggcgacggc ccagcccggc gacggggcgc gcgggtcgcg ccgcgactcg cgccagcgcg
     1321 ccgcggcgag cacgacgatg agcacgccga cgcccgtggc caggtagcgg tggaccatct
     1381 cgatccatgc cttgccatgg gtgaccggac cggtgggcag cgcctcctgc gccgcctgga
     1441 tctcggcgtg cgcgcccagc gggctgacct gcccgtagca gccgggccag tccgggcagc
     1501 cgagccccga gtcggtcagg cgcgtgaatg cgccgaacag caccaggtcg aaggtgagga
     1561 acagcgtgag cgccgtgagc gcctgcagcc gcgctgccgg cgacccgccg cgatgacgca
     1621 gccagatcca tgcgagcggg ccgagcgcca gcacggcgcc cagtgccgcc atgcgggcga
     1681 gcggtgcgag gtcgatcggc gcagccatcg cgagcgtgtc ctcagcgccc cgcgcggtcc
     1741 cagcccgccg acgcgcgcag cagccggtcc aggtcgcgct tgacgcgcga cgcgtcgggc
     1801 gaggctggct cgcgcatcat ccagcggccc atcgggtcca cgacgtacag atggtcgccg
     1861 agcgcgtggc ccggcgccgg ctccagccag cgcgccagcg cctcggcggg cacgcgcagc
     1921 accgtcaccg ggtcgccgtg cgcgaccgcc tgcagcactt cggcgcgcgg cgcggcggcg
     1981 tcggacacga gccacacctt gtcgacccgc cccttctcgg gccccagcgc ttcgcgcaat
     2041 tgccgctgca gcaggagctg ggattcgcac gtgcgctcgc acgccgcgcc gcccacgacc
     2101 accagcagcc actgcccctg caggctcgac ggcgccaccg cctcgccggc cagcgtggcg
     2161 agcggcaggc cgtcgggcgc cgcgcgggcc ggggtgacca gctcgccgta gttcgtgcgc
     2221 gcctgcggtc gaaccacgaa gtacgtgaag tacgacgcca cgaccggcgc cgcgcacacc
     2281 gccaggatgc ccagcatctt ccagcgaccg ctcgccgtgc gccgcgcctc gtcgtccgcg
     2341 agcgcgggcg acggcagcga gtgcaccgtg aaggcgagcg gcgcgtcagt ggcgacgctg
     2401 ccgccgcggc cggaggagtt ggaaccagac atacagtcct gcgatgaggg cggccagcgc
     2461 gaaccactgg aaggcgtagc cgtagtgctt gtggacgtcg gccgccgggg cgggccactg
     2521 gcgcgacagc ccgtcgtcgg ggccgctcgt ctgcaccacg gaaagcggcg cgagcggcac
     2581 gccggtctcg ccggcgaacg cggccaggtc gagattctga cggatcacgc cgccggccgc
     2641 cggcgcgagc tccaggagcc gcgacggggg cggggcgatg cggccctcga ccaccgcggg
     2701 acccgcgggc gcgggcaccg ggcgcagccg ggtgcggtcc gcggggtcgc gcggcatcca
     2761 gccgcgctgc accaccaccg cgaccgccgc gccgtccggc agcagcgggg tgacgacgaa
     2821 gaaacccggg cggccgttca tctggcggtt gtccaggaag accgtgaagc ggtccagcca
     2881 tcgcccgtgc agccgcacgc gacggtggtg ctggctccgc gccgcgtcgg ccgtggtggc
     2941 gaggtcggcg gcgccgagcg gggccagcgc ggcgcgcgtg tccagcgcct cctgcagcgc
     3001 gcgcttctgc gccgcgcgat cgagctgcca cgcgccgagc gagacgctcg ccgccactgc
     3061 ggcgagcgcc gcgagcagga cgccataatg gcttcgaatg aaggtcgtca tcatcgtcgc
     3121 gttcatcgcg atcctgggcg cgctggcctt cgccggcgtc gcgatgatcc gcggccgcga
     3181 gcaggaggga aagccgaagc cgaagacgat ggcgcgcgcg ctcgcctggc gcatcggcct
     3241 gtcggtggcg ctgttcctgc tcatcctgtt ggcctatcgc ttcggctgga tccagccgac
     3301 gggcgttccc acggggcgct gaccgcgccc tcgctcccgc gggcggcagc ggcggaggcg
     3361 gtcagaacca gtagacgatc acgtacagcc cgagccacac gacgtcgacg aagtgccagt
     3421 accacgccgc gccttcgaag ccgaagtgcc gctgcggcgt gaagtgtccc ttcatgatgc
     3481 gcagggtgat gaacagcagc atcagcatgc ccacgaacac gtggaagccg tggaagccgg
     3541 tgagcatgaa gaaggtggaa ccgtagatcc cggagttcag cttgaggttg aggtcctggt
     3601 acgcgtgcat gtactcgtag atctgcaggc tcaggaacgt cacgcccagc agcaccgtga
     3661 tccacatcca gaacaccgtg gcgccgcgcc ggccggcgat gagcaggtgg tgcgcgatcg
     3721 tgagcgtcac gccggaggcg agcagcaagg cggtgttgat cgtggggatc ggccacgggc
     3781 ccatcgtcgt gaagggctcg acgatgccgg ctggcgacgc cgtcgcgccc ggctgcacgc
     3841 tcggccacac ggccttgaag tcgggccaga ggatggcgtt ctgcaggctt ccgagaaccg
     3901 ggatggtgta cagccgcgac cagaacagcg cgccgaagaa ggcgccgaag aacatcacct
     3961 ccgagaagat gaaccagctc atgctccagc ggaacgacac gtcgacgcga tcgccgtaga
     4021 ggccgccttc gctctcgctg atcgcctggc ggaaccaccc ctgcatcgtg aaggcccaga
     4081 acagcaggcc cagcagcagc gaatacttgc cccaggacgc cccgttgacc cattgccccg
     4141 caccgaagat gacgaagaac aggccgatgg cggccatcac cgggaagcgc gatggcgccg
     4201 ggacgaagta gtaaggggtg tgcccgtgag gggtcgctgc cgacattgcc gtttctcctg
     4261 aaatccgtct cgctgttgtt ccgttccatc cgcggcacgc gcaccgcgcg ccgtccgaaa
     4321 acctagctcg ccacgccgct ggagatcacc cagttcacca gcagcaccag caccacgatg
     4381 aacagcaccg cggcgacgat gcccgcgacg atcacgtgca ccgggttgag ctggctcacg
     4441 tccttctcgt actccgccga cttgcgcacg ccgaagaacg accatgccac cgcgcgcatc
     4501 gtctgcagga acgagccctt gcggcgcacc gcgtgcttga gaccgccgtt gtccgcgggg
     4561 ctcatcgatg caccctcgcg cgccgcgcgg tcccgccggg aacggcgttg cgcgcccttc
     4621 gatgcgtccg ggcggcggcg ctcacgagcg cgcctcctgc gccggcgggt tcgcggcgcg
     4681 cgccgcgggc gccggcggga ccctgccccc gacctcgaag aacgtgtagg acagcgtgat
     4741 cgtgctcacg tccttcggca gcctggggtc gatgacgaac accaccggcc agcgccggac
     4801 ctcgcccggc tgcagcgtgt actcgttgaa gcagaagcat tccagcttgt cgaagtaggg
     4861 cgacgcctgc ttcggcgcgt agctcgggat cgcctgggcc gccatggcgc gcggctgcag
     4921 gttgcggaac tcgtacatca cggtcgtcat ctcgcccgga tgcacctcga cgctgcgctg
     4981 ctcgggcttg aagtcccaca cgccgcgggc gttggcgtcg aactccacgg tcaccttgcg
     5041 cgagcggtcg acctgcgtgc cggccgccgg cgcggccgcc gcgcccgggc gcgtgcgctc
     5101 ggccagcgac agcacgttca cccccagcgc ctcgcagatc gagcggtaca ccggaaccag
     5161 cgcatagccg aagccgaaca tcgccgcggt gaccacgacg agcttgccga ccatgcgccg
     5221 gttgtcgcgg cgcaggccgg cgtcgtgcgg tgcggtggag cgcggtggcg cgttcatgtg
     5281 cgggcccggc acggtcagat cccgagcagc gcggccttcg cgacgaagcc cacgaagagc
     5341 gccgccacca cggacgcgag gatccagccg aggcgctggt tctgcttgcg gcggtcggtc
     5401 atgcgtgatg gggggccgcg gtggcgctgg cgccgatcac cttggtggcc gtggcgttga
     5461 gccgcggcgg cgtctcgaag gtgtggtacg gcgccggaga cgggatttcc cactccaggc
     5521 cctcggcgcc ttcccagggc tgttgcggcg ccggggcgcc ccggccgcgc atcatcggca
     5581 gcacgaccat gaggaagaag tacacctgca tgagcccgaa gcccagggcg cccaccgacg
     5641 ccaccacgtt gaagtcggtg aactgcagcg ggtagtcggc atagcggcgc ggcatgccgg
     5701 ccaggccgag gaagtgcatc gggaagaagg tgacgttgaa gaagatgatc gagccccaga
     5761 agtggatctt gccgcgcgac tcgtcgtaca tcacgccggt ccacttcggg ccccagtagt
     5821 acacgccgcc gaacagcgcg aacagcgagc ccgccaccag cacgtagtgg aagtgcgcca
     5881 cgacgtagta cgtgtcctgg agctggatgt cgatcggcgc catggcgagg atgaggccgg
     5941 tgaagccgcc gatggtgaac acgaagatga agcccaccgc gaagagcatc ggcgtctcga
     6001 aggtcatcga cccgcgccac atcgtggcga cccagttgaa gatcttcacg ccggtgggca
     6061 ccgcgatcag catggtggcg tacatgaaga acagctgccc cgtcaccggc atgccggtgg
     6121 tgaacatgtg gtgcgcccag acgatgaacg acaggatggc gatcgacgcc gtcgcgtaca
     6181 ccatcgaggt gtagccgaac aggcgcttgc gcgcgaaggc cggcacgatg gcgctgatga
     6241 tgccgaacgc cggcaggatc atgatgtaga cctcggggtg gccgaagaac cagaagatgt
     6301 gctggtacat caccgggtcg ccgccgcccg cggggttgaa gaaggaggtg ccgaagtgcc
     6361 ggtcggtgag cgtcatcgtg atggcgccgg cgagcaccgg catcacggcg atcagcaggt
     6421 acgcggtgat gagccacgtc cagcagaaca gcggcatctt catgagcgtc atgccgggcg
     6481 cgcgcat
//
LOCUS       DASRIE010000193         8662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3060690, whole genome shotgun sequence.
ACCESSION   DASRIE010000193 DASRIE010000000
VERSION     DASRIE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060690"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFB53_07970"
     CDS             <1..366
                     /locus_tag="VFB53_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN86707.1"
                     /translation="PVPEPGGLGVHVTLDLGGRARFGPDVEWVEAIDYRVDPRRAERF
                     YAAIRRYWPGLPEGALAPGYAGIRPKISAQGEPAADFLVQGPRAHGVAGLVHLFGIES
                     PGLTASLALADDVAAQLPH"
     gene            380..1345
                     /locus_tag="VFB53_07975"
     CDS             380..1345
                     /locus_tag="VFB53_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZN86708.1"
                     /translation="MKARIAVLLAASAAFGVAQAQTKWDMPTPYSDGEFHTRNIKQFA
                     EDVKQATKGGLDITLHTAGSLIKHPDILRAVSTGQVNIAEFLLGQFGNEDPVFAADNV
                     PFVATGYDNAWKFYQAQKPLLTKKLEGRGLTLLYSVPWPGQGIYTKEPLKSVADLKGT
                     KFRTYSPLTARLAELLGASPTVIQVPEVPQMFATGAVHAMITSSATGTSTKAWEFVKN
                     YYTTSAFHPKNVVVANARAFARLPKAQQAAVLAAAKAAEPRGWEMSKAREKEGDELLA
                     KNGMAVRAPDQGMKDAFAKVGAQMAQEWEKSAGADGQAILKAYKK"
     gene            1350..1883
                     /locus_tag="VFB53_07980"
     CDS             1350..1883
                     /locus_tag="VFB53_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZN86709.1"
                     /translation="MRRFLDRLYAASGALAALCLAGIALMMLAQAFGREAGVLVRGAD
                     DIVSWLCAASAFLALGHTFRRGELVRVGLWLDMLGARARWIAELCALGVTALFAGYMV
                     WSVLRFVYESWQFNEVAQGLIQLPIWIPQMSFVAGALIFLAAVLDELVAVARKRKPAY
                     QLAEEERRAAGDFSETV"
     gene            1884..3197
                     /locus_tag="VFB53_07985"
     CDS             1884..3197
                     /locus_tag="VFB53_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZN86710.1"
                     /translation="MTVLGSSLVLLALLLVLLVGGVWIAVALAITAWFGLQAFTSSPP
                     DVNLFQSFWGSSASWTLAALPMFVWMGEILYRSKLSEQMFEGLAPWLGWLPGRLMHVN
                     IFGCAIFGTVSGSSTATCATIAKVALPELKKRGYDEKTALGSLAAAGTLGILLPPSII
                     MVVYAVAAEVSIIKVFIAGLLPGLVLTLLFSGYIVAWALLNPGKTPRDEFRLPLREKL
                     YRIRHLVPCLLLIVFIVWVMVIGWATATEAAAYGVLGALAIAGWSGGLNRATFWASLM
                     GATRLSCMILFILAGASFLSSCMAFTGIPRALAEWVASLQPNKYALIGILAGIYLLLG
                     TALDGVSMIVLTTSIVIPMIEKAGIDLVWFGIFIVLLVEIAELTPPLGFNIFVLQTMT
                     GRDSNYVALASLPFFLMMVLCIALITAFPELCTWLPDTLVGVTKK"
     gene            3215..4624
                     /locus_tag="VFB53_07990"
     CDS             3215..4624
                     /locus_tag="VFB53_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZN86711.1"
                     /translation="MSAPIYLSKDIRRIEQAAKDAPLMQRAGEAAAALAARLCADTAK
                     DVLVLAGPGDNGGDARIVAEVLRQQFFRVTVAGAAEAASLPVDRRWGLVVDGLFGIGL
                     ARDVEGEYARLIEYANSQDCAVLALDIPSGIAADTGRVLGRAVRATHTITFIALKPGL
                     LTLDGPDHCGAVTVADLGLDLKKLARPGAWVAHPQLFADVLKPRPRNFHKGKAGSLGI
                     LGGAAGMTGAALLAGRAALKLGAGRVYVGMLEETSIDPVTPELMLRHPDDALGQDLDA
                     MVIGPGLGRSERAQALLGAVLASELPCVLDADALNLLADDSELRATCQKRGADTLVTP
                     HPGEAARLLAVPVAAVQSDRLGAAQRIATELNAHVVLKGNGSVLVARDGHWFINTTGN
                     PGMASAGMGDVLSGILGALLAQRLSGEAALVLGVHLHGAAADAAGIGVGLTASELIEP
                     ARALWNRWLQGSGPEPGLH"
     gene            complement(4621..5142)
                     /locus_tag="VFB53_07995"
     CDS             complement(4621..5142)
                     /locus_tag="VFB53_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86712.1"
                     /translation="MTAIIAGLLAAAAAAALVQGIFGSGQEGAPGFTGVGVLIADVNL
                     ALQALLLLGLTFGFYLARRGSIEAHRVNQTVWVLVNTVLVVLIMLASMQDVRLGSLAD
                     LGNARAAVTWLHALLGAFTAVAGLWLVLQMNDLLPRRWHLAGWKTLMRLTLAGYWASA
                     LLGFGAYLTWYAG"
     gene            complement(5139..5486)
                     /locus_tag="VFB53_08000"
     CDS             complement(5139..5486)
                     /locus_tag="VFB53_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86713.1"
                     /translation="MRLLLVLLPLLPLLPLPALALTPAAQEFMDITARLEPVQCEKRQ
                     LRRKMALATAEQRGAELEKFRARFAELNGDRDTARLEQRLAELEKRISAARDPADLDA
                     ISLQQRQAFYRCD"
     gene            complement(5483..6964)
                     /locus_tag="VFB53_08005"
     CDS             complement(5483..6964)
                     /locus_tag="VFB53_08005"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZN86714.1"
                     /translation="MEVLSYGIGTLIAFAILLAVLFVFISDITQKKHGVLRNYPVVGH
                     LRYFFEQLGEYFRQYFFSGDREEMPFNRATRGWIYRLAKNEGGTIGFGSTYDLHQPGA
                     LIFVNHPFPVLEEERLPTPALALGEGYCREPFIGRSIVNVSGMSYGAISAPAVRSLSR
                     GAAVAGCWMDTGEGGLSPYHLEGGCDIVMQIGTAKYGVRDAQGNLSEERLLELSKVVK
                     AFEVKLSQGAKPGKGGVLPGRKVTAEIAQIRGIPEGRDSISPNRHRDIANMAELLDRL
                     AWIRNLTGRPVGVKTAVGGWRFMNEMAEIVHKKGLEFAPDFLVIDGGEGGSGAAPQAL
                     ADHMSLSIDEALPRVVDALVETGLRQRVRVVASGKIVTSARAAWALCVGADFINTARG
                     FMFSLGCIQALRCHTNTCPTGVTTHNKRLQRALVVEEKYLRVANYALNMNREIDMIAH
                     SCGVRHARELRREHVRIVQANHQSIAMNMLYPYPEPKARVKAA"
     gene            complement(7005..7475)
                     /locus_tag="VFB53_08010"
     CDS             complement(7005..7475)
                     /locus_tag="VFB53_08010"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN86715.1"
                     /translation="MLQPGQAAPTFVLPDADMQNVSLSAFKGRKHVVVYFYPRDGTPG
                     CILEAAEFSDHEAQFGKHDCVIIGISRDDCLSHAEFRDKHGLSIGLLSDTEGEVCRRY
                     GVLQEKETEGVRRECLVRSTFVIDKQGVIRHAHYGVAPRGHATEIYDVVKRLKS"
     gene            7572..8645
                     /locus_tag="VFB53_08015"
     CDS             7572..8645
                     /locus_tag="VFB53_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN86716.1"
                     /translation="MKQPLLGGLSAKDFLRRHWQKKPLLVRGALPGFRGVASRAQLRR
                     LAARPDVESRLVRRRGARWRVVHGPLACTALQRNSTLLVQGVNLHLAAADRLLRRFHF
                     LPQARLDDVMVSWAAPGGGVGPHFDSYDVFLLQGEGRRVWRLQRPRPWRLAEGAPLKQ
                     IEDFAPDEEILAEPGDLLYLPPGWGHDGAALDECFTCSVGLRAPQGAELAAAFLDYLH
                     ERGLPRADYRDPGLRPTARGAEIPREMAQHAARTLSRIRWSGSDAARFLGRFLTTPKP
                     HVVFRPPAQPLARAAFARRGTVVLDAKTQLLYRGKYCYCNGEEYAATPALRVLADQRR
                     ALVRAAQADLFYEWYLAGFLHPE"
BASE COUNT         1204 a         3127 c         2993 g         1338 t
ORIGIN      
        1 ccggtaccgg agcccggcgg gctcggcgtg cacgtgacgc tcgaccttgg cgggcgcgcg
       61 cgcttcggcc cggacgtcga gtgggtggag gcgatcgact accgggtgga tccgcggcgc
      121 gccgagcgct tctacgccgc gatccgccgc tactggcccg ggctgcccga gggcgcgctc
      181 gcgccgggct atgccggcat ccggccgaag atctccgccc agggcgagcc cgcggcggat
      241 ttcctcgtgc aggggccgcg cgcgcacggc gtcgcggggc tggtgcacct gttcggcatc
      301 gagtcgccgg gattgacagc gtcgctcgcg ctggccgatg atgtggcggc gcaacttccg
      361 cactaatcca ggggaatcca tgaaagccag aatcgcagtg ctgctcgccg cgtcggccgc
      421 cttcggcgtc gcccaggcgc agaccaagtg ggacatgccc acgccctact ccgacggcga
      481 gttccacacc cgcaacatca agcagttcgc cgaggacgtg aagcaggcga ccaagggcgg
      541 cctcgacatc acgctgcaca ccgcgggctc tctcatcaag catcccgaca tcctgcgcgc
      601 cgtctccacc ggccaggtga acatcgccga attcctgctc ggccagttcg gcaacgagga
      661 cccggtgttc gcggcggaca acgtgccctt cgtcgccacc ggctacgaca acgcctggaa
      721 gttctaccag gcgcagaagc ccctgctgac gaagaagctc gagggccgcg gcctgacgct
      781 gctgtattcg gtgccctggc ccggccaggg gatctacacc aaggagccgc tcaagtcggt
      841 cgccgacctg aagggcacca agttccgcac ctacagcccg ctcaccgcgc gcctcgccga
      901 gctgctcggc gcgagcccca ccgtgatcca ggtgcccgag gtgccgcaga tgttcgccac
      961 cggcgcggtg cacgcgatga tcacctcgtc ggccaccggc acctcgacca aggcctggga
     1021 gttcgtgaag aactactaca ccaccagcgc cttccacccc aagaacgtgg tggtggccaa
     1081 tgcgcgcgcc tttgcgcgcc tgcccaaggc gcagcaggcc gcggtgctcg ccgcggccaa
     1141 ggccgccgag ccgcgcggct gggagatgtc caaggcgcgc gagaaggaag gcgacgagct
     1201 gctcgccaag aacggcatgg cggtgcgcgc gcccgaccag gggatgaagg acgccttcgc
     1261 caaggtgggc gcgcagatgg cgcaggagtg ggagaagtcc gccggcgccg acggccaggc
     1321 gatcctcaaa gcgtacaaga agtagcggct tgaggcggtt tctcgaccgc ctctacgccg
     1381 cgagcggcgc gctcgcggcg ctctgcctcg ccggcatcgc gctgatgatg ctggcgcagg
     1441 ccttcgggcg cgaggccggc gtgctggtgc gcggcgccga cgacatcgtg tcgtggctgt
     1501 gcgcggcgag cgctttcctc gcgctcggcc acaccttccg ccgcggcgag ctggtgcgcg
     1561 tcggcctgtg gctggacatg ctcggcgcgc gcgcgcgctg gatcgccgag ctgtgcgccc
     1621 tcggcgtgac ggccctgttc gccggctaca tggtctggtc ggtgttgcgc ttcgtctacg
     1681 aaagctggca gttcaacgag gtggcgcagg gcctgatcca gctgccgatc tggatcccgc
     1741 agatgagctt cgtcgccggc gcgctgatct tcctcgccgc ggtgctcgac gagctggtgg
     1801 cggtggcgcg caagcgcaag cccgcctacc agctggcgga ggaggagcgc cgcgccgccg
     1861 gcgacttctc cgagacggtc tgaatgacgg tcctcggctc ctcgctggtc ctgctggcgc
     1921 tgctgctggt gctgctggtg ggcggcgtgt ggatcgccgt cgcgctcgcc atcaccgcct
     1981 ggttcgggct gcaggcgttc accagctcgc cgcccgacgt caacctgttc cagtccttct
     2041 ggggctcgag cgcctcctgg acgctggcgg cgctgccgat gttcgtgtgg atgggcgaga
     2101 tcctgtaccg ctcgaagctc tcggagcaga tgttcgaggg cctcgcgccc tggctcggct
     2161 ggctccccgg gcggctgatg cacgtcaaca tcttcggctg cgccattttc ggcacggtgt
     2221 cgggctcctc caccgccacc tgcgccacca tcgccaaagt ggcgctgccc gagctgaaga
     2281 agcgcggcta cgacgagaag accgccctcg gctcgctcgc cgccgcgggc accctcggca
     2341 tcctgctgcc gccctccatc atcatggtgg tgtatgcggt ggccgccgag gtctccatca
     2401 tcaaggtgtt catcgccggg ctcctgcccg ggctagtgct gacgctgctg ttctcgggct
     2461 acatcgtcgc ctgggcgctg ctcaatcccg gcaagacgcc gcgcgacgag tttcgcctgc
     2521 cgctgcgcga gaagctgtac cgcatacgcc acctggtccc ctgcctgctg ctcatcgtgt
     2581 tcatcgtctg ggtgatggtg atcggctggg ccaccgccac cgaggccgcc gcctacggcg
     2641 tgctgggcgc gctcgccatc gccggctggt cgggcggcct gaaccgcgcc accttctggg
     2701 cgagcctgat gggcgccacg cgcctgtcgt gcatgatcct gttcatcctc gccggcgcga
     2761 gcttcctctc ctcctgcatg gccttcaccg gcatcccgcg cgcgctcgcc gagtgggtgg
     2821 cctcgctgca gcccaacaag tacgccctga tcggcatcct cgccggcatc tacctgctgc
     2881 tcggcacggc gctcgacggc gtgtcgatga tcgtgctcac cacctcgatc gtgatcccga
     2941 tgatcgagaa ggccggcatc gacctggtct ggttcggcat cttcatcgtg ctgctggtgg
     3001 agatcgccga gctcaccccg ccgctcggct tcaacatctt cgtgctgcag accatgaccg
     3061 ggcgcgactc caactacgtg gcgctcgcct cgctgccctt cttcctcatg atggtgctct
     3121 gcatcgcgct gatcaccgcg ttccccgagc tgtgcacctg gctgcccgac acgctggtgg
     3181 gcgtgaccaa aaaatagggt cagaccccat tttcgtgtcc gcgcccatct acctgtcgaa
     3241 ggacatccgc cgcatcgagc aggcggcgaa ggacgcgccg ctgatgcagc gcgccggcga
     3301 agcggcggcg gcgctcgcgg cgcggctgtg cgcggacacg gcgaaggacg tgctggtgct
     3361 cgccggcccg ggcgacaacg gcggcgacgc gaggatcgtc gccgaggtgc tgcggcagca
     3421 gttcttccgc gtcaccgtgg ccggcgccgc cgaggcggca tcgctgccgg tggaccggcg
     3481 ctggggcctg gtggtggacg gcctgttcgg catcggcctg gcgcgcgacg tcgaaggcga
     3541 gtacgcgcgg ctcatcgagt acgccaactc ccaggactgc gcggtgctgg cgctcgacat
     3601 cccgagcggc atcgccgcgg acacgggacg cgtgctcggc cgcgcggtgc gcgccacgca
     3661 caccatcacc ttcatcgcgc tcaagcccgg cctgctcacg ctcgacggcc ccgaccactg
     3721 cggcgcggtg acggtggcgg acctcggcct ggacctgaag aagctggcgc ggcccggcgc
     3781 ctgggtggcg cacccgcaac tcttcgccga cgtgctcaag ccgcggccgc gcaatttcca
     3841 caagggcaag gcgggctcgc tcggcatcct cggcggcgcc gccggcatga ccggcgcggc
     3901 gctgctcgcg gggcgcgccg cgctcaagct cggcgccggc cgcgtctacg tcggcatgct
     3961 ggaggaaacg agcatcgacc cggtgacgcc cgagctcatg ctgcgccatc ccgacgacgc
     4021 gctcggccag gacctcgacg cgatggtgat cgggccgggc ctgggccgga gcgagcgcgc
     4081 gcaggcgctg ctcggcgcgg tgctggcgag cgaactgccc tgcgtcctcg acgccgacgc
     4141 gctcaacctg ctcgccgacg acagcgagct gcgcgcgacc tgccagaagc gcggcgctga
     4201 cacgctggtg acgccgcacc cgggcgaggc ggcgcggctg ctggccgtcc ccgtcgccgc
     4261 agtgcagagc gaccgccttg gcgccgcgca gcgcatcgcc acggagctga acgcgcacgt
     4321 ggtgctgaag ggcaacggca gcgtgctcgt ggcgcgcgac ggccactggt tcatcaacac
     4381 cacgggcaat cccggcatgg cctccgccgg catgggcgac gtgctctcgg gcatcctcgg
     4441 cgcgctgctg gcgcagcggc tctcgggcga ggccgccctg gtgctcggcg tgcacctgca
     4501 tggcgccgcc gccgacgccg cgggcatcgg cgttggtctc accgcctcgg agctgatcga
     4561 gcccgcgcgc gcgctatgga accgctggct gcaagggtcc gggccggaac ccggtttgca
     4621 ctagcctgcg taccaggtga ggtaggcgcc gaatccgagc agcgcgctcg cccaataccc
     4681 cgccagcgtc aggcgcatca gggtcttcca cccggcgagg tgccagcgcc gcggcaggag
     4741 gtcgttcatc tgcagtacca gccacagccc ggcgaccgcc gtgaacgcgc cgagcagcgc
     4801 atgcagccag gtgacggcgg cgcgcgcatt gcccaggtcg gcgaggctgc cgagccgcac
     4861 gtcctgcatc gacgcaagca tgatcagcac caccagcacc gtgttcacca gcacccagac
     4921 ggtctggttc acccggtgcg cttcgatgct gccgcggcgc gcgagataga agccgaaggt
     4981 caggccgagg agcagcagcg cctgcagcgc gaggttcacg tcggcgatga gcacgcccac
     5041 gccggtgaat cccggcgcgc cctcctggcc gctgccgaag atgccctgca ccagggccgc
     5101 cgccgcggcc gccgccagca ggccggcgat gatcgccgtc aatcgcaccg gtagaaggcc
     5161 tgccgctgct gcaggctgat ggcgtcgaga tccgccgggt cgcgcgcggc gctgatgcgc
     5221 ttctccagct cggccaggcg ttgctcgagc cgcgcggtgt cgcggtcgcc gttcagctcg
     5281 gcgaagcgcg cgcggaattt ctccagctcg gcgccgcgct gctcggccgt cgccagcgcc
     5341 atcttgcgcc gcagctggcg cttctcgcac tgcaccggct cgagccgcgc cgtgatgtcc
     5401 atgaactcct gcgcggcggg cgtgagcgcc agcgccggca gcggcagcag cggcagcagc
     5461 ggcagcagca cgagcagcag cctcacgccg ccttcacccg ggccttgggt tccggatagg
     5521 ggtacagcat gttcatggcg atcgactggt ggttggcctg cacgatgcgc acgtgctcgc
     5581 ggcgcagctc gcgcgcgtgc cgcacgccgc agctgtgcgc gatcatgtcg atctcgcggt
     5641 tcatgttcag ggcgtaattc gccacgcgca ggtatttttc ctccaccacc agcgcgcgct
     5701 gcagccgctt gttgtgggtg gtgacgccgg tggggcaggt gttggtgtgg cagcgcagcg
     5761 cctggatgca gcccagcgag aacatgaagc cgcgcgccgt gttgatgaaa tcggcgccca
     5821 cgcacagcgc ccaggcggcg cgcgccgagg tgacgatctt gcccgaggcc accacgcgca
     5881 cgcgctgccg caggccggtc tcgacgagcg cgtcgaccac gcgcggcagc gcctcgtcga
     5941 tcgacaggct catgtggtcg gcgagcgcct gcggcgccgc gcccgagccg ccctcgccgc
     6001 cgtcgatcac caggaaatcg ggcgcgaact ccaagccctt cttgtgcacg atctcggcca
     6061 tctcgttcat gaagcgccag ccgccgaccg cggtcttcac gcccaccggc cggccggtga
     6121 ggttgcggat ccacgccagg cggtccagca gctcggccat gttggcgatg tcgcggtgcc
     6181 ggttcgggct gatcgagtcg cgcccctcgg ggatgccgcg gatctgcgcg atctccgcgg
     6241 tcaccttcct gcccggcagc accccgccct tgccgggctt ggcgccctgc gacagcttca
     6301 cctcgaaggc cttcaccacc ttcgagagct cgagcagccg ctcctccgac aggttgccct
     6361 gcgcgtcgcg caccccgtac ttggcggtgc cgatctgcat cacgatgtcg cagccgccct
     6421 ccaggtggta aggcgacagg ccgccctccc cggtgtccat ccagcagccg gccaccgccg
     6481 cgccgcgcga cagcgagcgc accgccggcg ccgagatcgc gccgtagctc atgccgctca
     6541 cgttgacgat cgagcgcccg atgaagggct cgcggcaata gccctcgccc agcgccagcg
     6601 cgggcgtcgg caggcgctcc tcctccagca ccgggaacgg gtggttgacg aaaatcagcg
     6661 cgcccggctg gtgcaggtcg taggtcgagc cgaagccgat ggtgccgccc tcgttcttcg
     6721 ccaggcggta gatccagccg cgcgtggcgc ggttgaaggg catttcctcg cggtcgccgg
     6781 agaagaagta ctggcggaag tactcgccca gctgctcgaa gaagtagcgc aggtggccga
     6841 ccacggggta gttgcgcagc accccgtgct tcttctgcgt gatgtcgctg atgaacacga
     6901 acagcaccgc cagcaggatc gcgaacgcga tcagcgtgcc gatgccgtag gacagcactt
     6961 ccatcatctg gcggcccttt cccccgagcc gggatgatag cgcctcagga cttcaggcgc
     7021 ttgacgacgt cgtagatctc ggtggcgtgg ccgcgcggcg ccaccccgta gtgcgcgtgg
     7081 cggatcacgc cctgcttgtc gatgacgaag gtcgagcgca ccaggcactc gcgccgcacg
     7141 ccctcggtct ccttttcctg cagcacgccg tagcgccggc acacctcgcc ctcggtgtcc
     7201 gacagcaggc cgatggacag gccgtgcttg tcgcggaact cggcgtgcga caggcagtcg
     7261 tcgcgcgaga tgccgatgat cacgcagtcg tgcttgccga actgcgcctc gtggtcggag
     7321 aactcggcgg cctccaggat gcagcccggc gtgccgtcgc gcggatagaa atacaccacc
     7381 acgtgcttcc gtcccttgaa cgccgacagg ctcacgttct gcatgtcggc gtcgggaagc
     7441 acgaacgtcg gtgccgcttg tcccggttgc agcatggctg gtgttgtcgc gtccgattct
     7501 aatgcacatc gtttgcccgc aggcaagcgg gcggcgcgcg atgtcgcgcc gctgccacat
     7561 ataatcttcc gatgaaacaa ccgctgctag ggggcttgtc ggcgaaggat tttctgcgcc
     7621 gccactggca gaaaaaaccg ctgctggtgc gcggcgcgct gcccggattc cgcggcgtcg
     7681 cgtcgcgcgc gcagctacgg cggctcgccg cgcgccccga cgtcgagtcg cgcctggtgc
     7741 gccggcgcgg cgcgcgctgg cgcgtggtgc acggcccgct cgcgtgcacc gcgctgcagc
     7801 gcaactccac ccttttggtg cagggcgtca acctgcactt ggcggcggcc gaccggctgc
     7861 tgcgccgctt ccacttcctg ccgcaggcgc ggctggacga cgtgatggtg agctgggccg
     7921 cgcccggcgg cggcgtcggg ccgcacttcg actcctacga cgtgttcctg ctgcagggcg
     7981 agggccggcg cgtatggcgc ctgcagcggc cgcgcccctg gcgcctggcg gagggcgcgc
     8041 cgctcaagca gatcgaggat ttcgcgccgg acgaggagat cctcgccgag cccggcgatc
     8101 tgctctacct gccgccgggc tggggccacg acggcgcggc gctcgacgag tgcttcacct
     8161 gctcggtcgg gctgcgcgcg ccccagggcg cggagctcgc cgccgctttc ctcgactacc
     8221 tgcacgagcg cggcctgccg cgcgccgact accgcgaccc gggcctgcgc ccgacggcgc
     8281 gcggcgccga gatcccgcgc gagatggcgc agcacgcggc gcgcacgctg tcgcggatcc
     8341 gctggtcggg cagcgacgcg gcccgcttcc tcggccgctt cctcaccacg ccgaagccgc
     8401 acgtcgtatt ccggccgccg gcgcagcccc tggcgcgggc ggcgttcgcg cgccgcggga
     8461 cagtggtgct cgatgcgaag acgcagttgc tctatcgcgg caagtattgc tattgcaacg
     8521 gcgaggaata cgccgcgacg cccgctttgc gcgtgctggc cgaccagcgg cgcgcgcttg
     8581 tgcgcgcggc gcaggccgat ttattctacg agtggtacct ggcgggattc ctgcacccgg
     8641 agtgagtagc gtggccgacg aa
//
LOCUS       DASRIE010000194        11846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2011667, whole genome shotgun sequence.
ACCESSION   DASRIE010000194 DASRIE010000000
VERSION     DASRIE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011667"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VFB53_08020"
     CDS             complement(<1..1779)
                     /locus_tag="VFB53_08020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HZN86717.1"
                     /translation="MSKNLIIAEKPSVAADIARALGGFTRKGDYFESDEYVLSSAVGH
                     LLELSLPPDHDVKRGKWSFANLPVIPPHFELAPIERGAERLNLLLRLLKRKDVVGLIN
                     ACDAGREGELIFRYIVQYARSKKSIRRLWLQSMTTASIRDGFDRLRADKELLPLADAA
                     KSRSEADWLMGINGTRAMTAFNSREGGFYLTTVGRVQTPTLAILVEREERIREFKARD
                     YWEVHARFGAKAGEYAGRWFDPGFKKDDDGERKPERLWQAAAADAIAADCRGRPGTVS
                     EETKPTTQASPLLFDLTSLQREANGRFGFSARGTLALAQSLYERHKVLTYPRTDARAL
                     SEDYLGTVQKAMDMLAADREYAPFAKMVLKNGWVKPNRRIFDNSKISDHFAIIPTLQA
                     PKNLNEAERKLYDLVVRRFLAVFHPSAEFLQTTRITAVGEHRFKTEGKVLTSPGWLAV
                     YGRATEGENENLPPVGAGEKVKTLEAASAAQQTKPPARYSEATLLSAMEGAGKLVEDD
                     ELREAMEAKGLGTPATRAAIIEGLIREEYVHRNGRELVPTPKAFSLMFALHMLGIVEL
                     ASPELTGEWEHKLRLIEQGKLTREEFM"
     gene            complement(1849..2307)
                     /locus_tag="VFB53_08025"
     CDS             complement(1849..2307)
                     /locus_tag="VFB53_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HZN86718.1"
                     /translation="MFDVLVYLYANCHQADVSHDPDRVARKLSAAGFEEAEISAALHW
                     FAGVVRAPQRSLAPLPDRGRAFRAFAPKELAKLDVECRGFLLALEQSGILTPQQREHI
                     IERALATADEALALEQFKLIVLMVLWNQHAPSSQLVAAELMTAPGARPPN"
     gene            complement(2331..3416)
                     /gene="dprA"
                     /locus_tag="VFB53_08030"
     CDS             complement(2331..3416)
                     /gene="dprA"
                     /locus_tag="VFB53_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZN86719.1"
                     /translation="MTQDPGLASWLQLTLTPGLGAAAIRGLLQQFGLPEKVLGATRAQ
                     LERAAGPAAAQLLRPEGIAPAIERSLRWAEEPGHAIITLADAEYPRLLLEIQDPPPLL
                     YAIGQPALLQRPALAVVGSRNATTQGARNAEQFARAFSDAGLTIVSGLALGIDAAAHR
                     GGLAGAGSTIAVLGTGVDQVYPRQNAALAQHIAEAGLLLSEFPLGTPGAAHHFPRRNR
                     LISGLAQGCLVVEAALASGSLITARAAAEQGREVFALPGSIHSPLSKGCHALIKQGAK
                     LVESADDVLAELTAFRRSGYASTRAASAAPEDEPLLVQMGFDPVDVDTLCARSGLAAK
                     QVAAELLRLELAGQVAALPGGMYQRLA"
     gene            complement(3459..4640)
                     /locus_tag="VFB53_08035"
     CDS             complement(3459..4640)
                     /locus_tag="VFB53_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZN86720.1"
                     /translation="MHDQLRKSITALVFVVSGLAAGAAFAQVPATPIAFKQDAPDRYV
                     VVRGDTLWGISERFTDSPWRWPEIWNFNREQIRNPHWIYPGDVIVLDRVSGKLSIAGA
                     DGGPGAGAGAGAGAGADGGPGAAGAGDARRGLGAGGVVGTVKLSPRLRAESTARDAIP
                     SIPANAIEPFLSRPLVIEPDGLDNAPTIIATQENRVIIEAGNQAYVRGIGNSKEENWF
                     VYRRGRPLVDPDTNLTLGYEAIYLGTARLTRAGDPAIVQLTTVTQEVGTGDKLVPVGM
                     PEVPKYAPHAPAVFMQGRVMSIYGGLGKVGESGPQSIITLNRGRSDGVEVGHVFALYR
                     PGPVIADASKATGGAPATFKLPDERYGLAFVFRLYDRVSYALVMRISRPVRPLDVVQT
                     P"
     gene            4786..5289
                     /gene="def"
                     /locus_tag="VFB53_08040"
     CDS             4786..5289
                     /gene="def"
                     /locus_tag="VFB53_08040"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZN86721.1"
                     /translation="MSLLNILRYPDGRLHKVASPVTVFDEPLRKLVRDMAETMYAAPG
                     VGLAATQVDVHKQVIVLDVSERHDSLVVLVNPEIVDASGISDIEEGCLSVPGVYDVVE
                     RAERVRVRACDPSGKPFTLEAQGLLAVCIQHEMDHLKGKVFVEYLSQLKQQRVRAKLA
                     KQLRKTA"
     gene            5296..6219
                     /gene="fmt"
                     /locus_tag="VFB53_08045"
     CDS             5296..6219
                     /gene="fmt"
                     /locus_tag="VFB53_08045"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZN86722.1"
                     /translation="MRLVFAGTPEFAERALSALLDAGHAVSLVLTQPDRPAGRGMRAS
                     PSAVKQLALARGIEVFQPAALREPAALERIRAAEPDALVVAAYGLLLPRAVLDVAPQG
                     ALNIHASLLPRWRGAAPIQRALIAGDRETGISIMRMDAGLDTGPVLARQRIAIDTQDD
                     AQTLHDKLAALGARMIVAALAEVAAGRARAVPQPEDGATYARKIDKREALIDWQAPVV
                     EVERLVRALRPWPGAAAPWRGEALKIWRATADAAGGKPGTVLESGPSGILVACGKGAL
                     RILELQRAGGRRLGADAFLRGCAIDAGERLQ"
     gene            6216..7475
                     /gene="rsmB"
                     /locus_tag="VFB53_08050"
     CDS             6216..7475
                     /gene="rsmB"
                     /locus_tag="VFB53_08050"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZN86723.1"
                     /translation="MKRNADLAPALAEAARAVARVGAGRSLDDAFERRAGRGALLDLC
                     YGTLRRYGRVQAIVQALSHKGAPEPRVQALLWCALYALDCGRYAEYTVVDQAVRACAL
                     LEQWPARGYVNGMLRGYLRSRGALERRIAAEPQARYQHPQWWIDSLQRAYPERWGAIL
                     EAGNTHPPMCLRINRRRTDQPSYLQQLAQAGIAACPAGAHGLLLDKPVPVERLPGFDQ
                     GLVSVQDAGAQHCAAMLGLRDGQRVLDACAAPGGKSAQLLESAEIRLLALDSDAARAA
                     RIGPGLARLGLEARVQIADCTAPPSWWDGQPFDRVLADVPCTASGIARRHPDIKWLRR
                     ADDVAAFARRQAAILDALWGVLAPGGKLLYVTCSVFPEENGMVLDAFCARVPQARRPA
                     LPAGAPAQLLPCAQHDGFFYGLLEKAG"
     gene            7493..8113
                     /locus_tag="VFB53_08055"
     CDS             7493..8113
                     /locus_tag="VFB53_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZN86724.1"
                     /translation="MSATCARFLVALACAASLCARADDIEVLDARLAASEEGLLLSAD
                     FAFEFNSRLNETVANGVPLYFVVEFELTRPRWYWWDEHTVSRATQLRLSYHALSRQYR
                     LTTGPLHQNFATLDEALDVMRRLRNWQVLGRSATLRSTSYDAAVRMRLDTAQLPRPLQ
                     VSALTSRELRLESPWKRFIFAPPAPPPPAPPPAPAEAREAQEGSPR"
     gene            8110..10233
                     /locus_tag="VFB53_08060"
     CDS             8110..10233
                     /locus_tag="VFB53_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86725.1"
                     /translation="MRSLLIVGAALAGVLIFLLATASAADSTMLAQHYPLLLGLNAAL
                     AAALAALVAYQLAGVVRRYRGRAFGSRLTLRFLLLFAMMAVVPGVLVYTVSVQFLTKS
                     IESWFDVKVDAALEGGISLGQSAIDRMLAEMQTKGRAMALELAERSPAQQVALLNRLR
                     EQAGVQEAVLVGANGTLVASATEDVARFLPELPTAQMLRQARSARGYTSIDALQGRPL
                     ALRVVLPVTGLAIADESRFLQLRQMVPAGLARSAEAVEAAYRDYRELALSRQGLRRIY
                     IVTLTLALLMGLFVAVSLAAILSARLSAPLANLAQATQAVARGDFSQRPPVTSRDELG
                     VLTESFNSMTRQLDEARKVVESNRIALETANASLENILANLSAGVLVFDRTLRLTISN
                     RGAAEILGNELQAHAGRPLAAWSAALETFAAGVRAQFLEHGERTWQQEISLRGTGKTL
                     LARGAALPEATGGGYVLVFDDITQLIQAQRATAWAEVARRLAHEIKNPLTPIQLSAER
                     LEMKLEGRLAPDDAEVVKRSTQTIVNQVTALKSMVDDFGDYARLPAPVPAGLDLNALI
                     TDVLALYETSRVPITARLAPGLPPVWADTAQIRQVIHNLVQNAQDALENRKEPRLLPA
                     IEVGTELVGDKLRLSVTDNGDGFPEELMTRIFEPYVTTKPRGTGLGLAIVKKIVDEHR
                     GSIAIENRPDSGASVSVLLPLAQAA"
     gene            10241..10588
                     /locus_tag="VFB53_08065"
                     /pseudo
     CDS             10241..10588
                     /locus_tag="VFB53_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            10637..10987
                     /locus_tag="VFB53_08070"
                     /pseudo
     CDS             10637..10987
                     /locus_tag="VFB53_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11303..11488
                     /locus_tag="VFB53_08075"
                     /pseudo
     CDS             11303..11488
                     /locus_tag="VFB53_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            11532..>11846
                     /locus_tag="VFB53_08080"
     CDS             11532..>11846
                     /locus_tag="VFB53_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN86726.1"
                     /translation="MKIVILGAGQVGSSVAESLVSEQNDITVVDVEPGRLRALQDRLD
                     LRTVIGSASHPSVLIEAGIEDADLLIAVTQSDETNLVACKLAARMFNVPRRIARIRAN
                     AFL"
BASE COUNT         1723 a         4091 c         4164 g         1868 t
ORIGIN      
        1 catgaactct tcgcgcgtca gcttgccctg ctcgatcagc cgcagcttgt gctcccattc
       61 gcccgtcagc tccggcgagg cgagttcgac gatgccgagc atgtgcaggg cgaacatcaa
      121 cgagaacgcc ttgggcgtcg gcaccagctc cctgccattg cggtgcacgt actcctcgcg
      181 gatcagcccc tcgatgatcg ccgcccgcgt cgccggcgtg cccaggccct tggcctccat
      241 ggcctcgcgc agctcgtcgt cctccaccag cttgccggcg ccttccatgg ccgacagcag
      301 cgtggcttcg ctgtaccgcg ccggcggctt ggtctgctgg gcggcgctcg ccgcctccag
      361 cgtcttgacc ttctcccctg cgcccaccgg cggcaggttc tcgttctcgc cttccgtggc
      421 gcggccgtag accgccagcc agcccgggct cgtcaggacc ttgccttccg tcttgaagcg
      481 atgctccccc accgcggtga tgcgcgtggt ctgcaggaat tcggccgacg ggtggaacac
      541 ggcgaggaag cgccgcacca ccaggtcgta gagcttgcgc tcggcctcgt tcaggttctt
      601 gggcgcctgc agcgtgggaa tgatggcgaa gtggtcagag atcttgctgt tgtcgaagat
      661 gcggcggttg ggttttaccc agccgttctt cagcaccatc ttggcgaacg gcgcgtactc
      721 gcggtcggcg gcgagcatgt ccatcgcctt ctgcaccgtg ccgaggtaat cttccgacag
      781 cgcgcgcgcg tccgtccgcg gataggtgag caccttgtgg cgctcgtaaa gcgactgcgc
      841 cagggccagc gtgccccgcg ccgagaagcc gaagcgcccg ttcgcctcgc gttgcaggct
      901 ggtcaggtcg aacagcaggg gcgaggcctg cgtggtcggt ttggtttcct cgctgaccgt
      961 gccgggcctg ccgcggcagt ccgcggcgat cgcgtcggcc gcagccgcct gccacaaccg
     1021 ctcgggcttg cgctcgccgt cgtcgtcctt cttgaagccg gggtcaaacc agcggcccgc
     1081 atactcgccg gccttggcgc cgaagcgtgc atgcacttcc cagtagtcgc gcgccttgaa
     1141 ctcgcgaatg cgctcctcgc gctccaccag gatggccagg gtcggcgtct gtacgcggcc
     1201 cacggttgtg aggtagaagc cgccttccct ggaattgaac gcggtcatgg cgcgcgtgcc
     1261 gttgatgccc atcagccaat cggcctccga gcggctcttg gcggcatcgg cgagcggcag
     1321 cagctccttg tcggcgcgca gccggtcgaa gccgtcgcga atcgaggcgg tggtcatcga
     1381 ctgcagccac aggcgccgga tcgatttctt gctgcgcgcg tactgcacga tatagcggaa
     1441 gatcagttcg ccctcgcggc ccgcgtcgca ggcattgatc aacccgacaa cgtccttgcg
     1501 cttgagcagg cgcagcagca ggttgagccg ctcggcgccc cgctcgatcg gcgccagctc
     1561 gaaatgtggg gggatgaccg gcaggttggc gaacgaccat ttgccgcgct tgacgtcgtg
     1621 gtccggcggc agggagagct cgagcagatg gccgacggca gacgacagga catactcgtc
     1681 gctttcgaag taatcgccct tgcgcgtgaa gccgcccagc gcgcgcgcga tgtccgcggc
     1741 gacggaaggc ttctcggcaa tgatcagatt cttgctcatg cggcgcgaaa ggccgcagag
     1801 gatacccgca aagatcgcgc ttttacaacc gcggcgcgcg cgcggcgctc agttcggcgg
     1861 cctggcgccc ggcgccgtca tcaattcggc cgccaccagt tggctggacg gggcatgctg
     1921 gttccacagc accatcagca cgatcagctt gaattgctcg agggccagcg cctcgtccgc
     1981 ggtcgcgagc gcgcgttcga tgatgtgctc gcgctgctgc ggcgtgagga ttcccgactg
     2041 ctccagggca agcaggaagc cgcggcactc gacgtcgagc ttggccaatt ccttgggtgc
     2101 gaaggcgcgg aacgcccggc cccggtcggg cagcggggcg agtgaacgct gcggcgcgcg
     2161 taccacgccg gcgaaccagt gcagcgcggc gctgatctcc gcctcctcga accctgccgc
     2221 cgagagcttg cgcgccaccc tgtccgggtc gtgcgacacg tcggcttggt ggcagttcgc
     2281 gtacaggtag acgaggacat cgaacatgtt cttgttctcc cggttgaggt tcaggcaagc
     2341 cgttggtaca tcccgcccgg cagcgcggcg acctgccccg ccagctcgag ccgcagcaat
     2401 tccgccgcca cctgcttcgc cgccaacccg gaccgcgcgc acagcgtatc cacgtccacg
     2461 ggatcgaacc ccatctgcac cagcagtggt tcatcctccg gcgccgcgct cgccgcgcgc
     2521 gtggaggcgt atccgctgcg ccggaaggcg gtgagctcgg ccagcacatc gtcggcggat
     2581 tcgaccagct tggcgccctg cttgatgagg gcgtggcaac ccttggacag gggcgagtgg
     2641 atcgatcccg gcagcgcgaa cacctcgcgc ccctgctcgg ccgccgcgcg tgcggtgatg
     2701 agcgagccgg aggcgagggc ggcctcgacc accagacagc cttgcgcgag tccgctgatc
     2761 aggcggttgc ggcgcgggaa gtggtgcgcc gcgcccggcg tgccaagggg aaattccgag
     2821 agcagcaggc cggcttcggc gatgtgttgc gccagcgcgg cgttttgccg cgggtagacc
     2881 tggtcgactc cggtgcccag caccgcaatg gtcgaaccgg cgcccgccag tccgccgcga
     2941 tgcgcggccg cgtcgatgcc gagcgccagt cccgacacga tggtcaaccc ggcgtcactg
     3001 aacgcccggg cgaactgctc ggcgttgcgc gcgccctggg tcgtcgcatt gcggctgccc
     3061 accacggcga gcgcgggacg ctgcagcagg gccggctggc cgattgcgta cagcagcggc
     3121 ggcggatcct ggatttccag caacagcctg ggatattccg cgtcggcgag cgtgatgatg
     3181 gcgtgtccgg gttcctccgc ccagcgcagg ctgcgttcga tcgccggggc tatgccttcg
     3241 ggtcgcagca gctgcgccgc tgcgggaccg gcggcgcgct ccagttgcgc gcgcgtggcg
     3301 ccgagcacct tctcgggcag gccgaactgc tgcagcaggc cgcgtattgc ggcggcgccg
     3361 agcccgggcg tgagcgtgag ttgcaaccag cttgccagcc cgggatcttg cgtcatcccg
     3421 gaggcaaggt gcggagggac tgtcccccgc agttcaggtc agggggtctg aacgacatcc
     3481 agcggtctta ccggcctgga tatgcgcatc accaaagcgt aggagacccg gtcatagagg
     3541 cgaaacacga atgcgagccc gtagcgctcg tcgggaagct tgaaggtcgc cggcgcgccc
     3601 cccgtcgcct tggaggcgtc ggcgattacc gggccggggc ggtacagcgc gaagacatgg
     3661 cccacttcca cgccgtcgga gcggccccga ttcagcgtga tgatggattg cggtccggac
     3721 tcgccgacct ttcccaggcc gccgtagatg ctcatcaccc tgccctgcat gaatacggcc
     3781 ggggcatgcg gcgcgtattt cggcacttcc ggcatgccta ccgggaccag cttgtcgccg
     3841 gtgccgactt cctgcgtgac ggtcgtcagc tgcacgatcg cagggtctcc ggcgcgggtg
     3901 aggcgcgccg tgccgaggta gatggcctcg tatcccagcg tgagattcgt atccgggtcg
     3961 acgagcggcc tgccgcggcg atagacgaac cagttctctt ccttcgaatt gccgatgccc
     4021 cgcacgtagg cctggtttcc cgcttcgatg atgacgcggt tctcctgcgt ggcgatgatg
     4081 gtcggcgcgt tatccaggcc gtcgggctcg atcaccaagg ggcgcgacag gaaaggctcg
     4141 atcgcattgg cgggaatgct cgggatggcg tcgcgcgcgg tcgactcggc gcgcaggcgc
     4201 ggcgagagtt tgaccgtgcc gacgacaccg ccggcgccga gaccgcgccg cgcgtcgccc
     4261 gcaccagccg cgccggggcc tccgtccgcg cccgcaccgg cgcccgcacc ggcgcccgcg
     4321 ccggggccgc cgtccgcgcc cgcgatggac agcttgccgc tgacccggtc cagcacgatc
     4381 acgtcgccgg ggtagatcca gtgcgggttt ctgatctgct cgcggttgaa gttccagatt
     4441 tcgggccagc gccacggcga atcggtgaaa cgctcggaga tgccccaaag ggtgtcgccc
     4501 cgcaccacga cgtagcgatc gggggcgtcc tgcttgaacg caatcggggt tgccggaacc
     4561 tgcgcaaagg ccgccccggc ggccaggccg gatacgacaa aaacgagcgc tgtgatagac
     4621 ttacgcaatt ggtcgtgcat ggtgcccccg aaccttgtcc atgtccccgg gctgccttga
     4681 aaagtccatt ggccagctaa atttagcaca aacggcacat tgtttatctg ctgccttaga
     4741 ttctgcatct aatcagttca atttgcaaca ctatttgcca atccgatgtc cctactgaat
     4801 atcctccgct accctgatgg ccggctccac aaggtcgcct ctcccgtaac cgtattcgac
     4861 gagccgctga ggaagctggt gcgcgacatg gcggaaacca tgtatgcggc gcccggcgtg
     4921 ggcctggccg ccacgcaggt cgacgtgcac aagcaggtta tcgtgctcga tgtctccgag
     4981 cgccatgatt ccctggtggt cctggtgaat ccggagatcg tcgatgcttc cggaatttcg
     5041 gacatcgagg aaggctgcct gtcggtgccc ggcgtgtacg acgtcgtgga gcgcgccgag
     5101 cgcgtgcggg tgcgcgcctg cgatccgagc ggaaagccgt tcacgctcga ggcccagggc
     5161 ctgctggcgg tgtgcatcca gcacgaaatg gatcacctca aaggcaaggt gttcgtcgag
     5221 tatctctcgc agctcaagca gcagcgcgtt cgcgccaagc tcgccaagca gctgcgcaag
     5281 actgcctaag ccttcctgcg cctagttttc gcgggcaccc ctgaattcgc cgagcgcgcg
     5341 ctgagcgcgc tgctcgatgc cggccacgcg gtatccctgg tgctgacgca accggaccgc
     5401 ccggcggggc gcggcatgcg cgcgtccccc agcgccgtca agcaactggc gcttgctcgc
     5461 ggcatcgaag tattccagcc cgcggccctg cgcgagcccg ctgcgctgga gcggatccgc
     5521 gcggcagagc ccgacgccct ggtggtcgcc gcatacggcc ttctcctgcc gcgggcggtg
     5581 ctggacgtcg cgccccaggg agccctgaac atccacgcct cgctgctgcc gcgatggcgc
     5641 ggcgccgcgc cgatccagcg cgccctgatc gccggcgacc gtgaaaccgg catttccatc
     5701 atgcgcatgg acgcgggcct cgataccggc ccggtgcttg cgcggcagcg cattgccatc
     5761 gacacgcaag acgacgcgca gacgctgcac gacaagctcg ccgctctggg cgcccgcatg
     5821 atcgtcgccg cgctggcgga ggtcgccgcg ggacgcgccc gcgcggtgcc gcagcccgag
     5881 gacggggcga cctatgcgcg caagatcgac aagcgcgagg cattgatcga ctggcaggcc
     5941 cccgtggtcg aggtggagcg cctggtgcgg gcgctgcgcc cttggccggg cgcggcggct
     6001 ccctggcgcg gcgaggcgct caagatctgg cgtgcgacgg ccgatgccgc cggcggaaag
     6061 cccggaaccg tgctggagtc ggggccctcc gggatactgg tcgcctgcgg caagggagca
     6121 ctgcgcattc tggagttgca gcgcgcgggg ggacggcggc tcggcgccga cgcgttcctg
     6181 cgcggttgcg cgatcgacgc aggcgagcgc ctgcaatgaa gcgcaacgcg gatctcgcgc
     6241 cggcgcttgc cgaagcggcg cgcgcggtgg cgcgcgttgg cgccgggcgc agcctcgacg
     6301 acgcgtttga gcgccgcgca gggcgcggtg ccttgctcga cctttgctac ggcaccctgc
     6361 ggcgctacgg gcgcgtgcag gcgatcgtgc aggcgctgtc gcacaagggc gcacccgagc
     6421 cccgcgtgca ggcgctgctc tggtgcgcgc tgtatgcgct ggactgcggc cgctatgccg
     6481 agtacaccgt ggtcgatcag gcggtgcgtg cctgcgcgct gctggagcag tggcccgcgc
     6541 gcggctacgt caacggcatg ctgcgcggct acctgcggtc caggggggcg ctggagcgcc
     6601 gcatcgccgc cgagccgcag gcacgctacc agcatccgca gtggtggatc gattccctgc
     6661 agcgcgccta cccggagcgc tggggcgcga tcctcgaagc gggaaatacg cacccgccga
     6721 tgtgcctgcg catcaaccgg cggcgcaccg atcagccgag ctatctgcag cagctggcac
     6781 aggcagggat cgccgcctgc ccggcaggcg cgcacggcct gctgctggac aagccggtgc
     6841 cggtggagcg gctgccggga ttcgaccagg gcctggtgtc ggtgcaggac gccggcgcgc
     6901 agcactgcgc ggcgatgctc ggcctgcggg acggccagcg cgtgctcgac gcctgcgcgg
     6961 cgcccggcgg caagagcgca cagctgctgg agagcgccga gattcgcctg ctcgcgctcg
     7021 actccgatgc cgcgcgtgcg gcgcgcatcg ggcccggatt ggcgcggctc ggcctcgagg
     7081 cgcgcgtgca gatcgcggac tgcacggccc cgccatcgtg gtgggacggg cagccgttcg
     7141 accgcgtgct ggccgacgtt ccctgtaccg cgtcgggcat cgcgcgccgc catcccgaca
     7201 tcaagtggct gcgccgcgcc gacgatgttg ccgccttcgc gcggcggcag gcggcgattc
     7261 tcgatgcgct ttggggggtg ctcgccccgg gtggtaaatt gctgtatgtc acctgctcgg
     7321 tgtttccgga ggagaacggc atggtgctgg atgccttctg tgcgcgcgtg ccgcaggcac
     7381 ggcgccccgc gctgccggcc ggcgccccgg cgcaattgct gccgtgcgcg cagcacgacg
     7441 gttttttcta cggtctgcta gagaaggccg gctgacgcgg cgcgcgatgc gggtgagcgc
     7501 cacctgtgcc agattcctcg tcgccctggc ctgcgccgcc tcgttgtgcg ctcgcgccga
     7561 cgacatcgag gtgctcgacg cgcgcctcgc cgcctccgag gaggggctgc tgctcagcgc
     7621 ggatttcgcc ttcgagttca attcccggct gaacgagacg gtggccaacg gcgtaccgct
     7681 gtacttcgtg gtcgagttcg aactcacccg cccccgctgg tactggtggg acgagcatac
     7741 cgtgtcgcgc gcgacgcaat tgcgcctgtc gtaccacgcg ctgtcgcgcc agtaccgact
     7801 gaccaccggg cccctgcatc agaacttcgc cacgctggac gaagcgctcg acgtcatgcg
     7861 ccggctgcgc aactggcagg tcctgggacg ttcggcgaca ctgcgcagca cgtcctatga
     7921 cgctgccgtg cgcatgcgcc tggataccgc gcaattgccc aggccgctgc aggtgagcgc
     7981 gctcaccagc cgggaactgc gcctggagtc tccctggaag cgcttcattt tcgccccgcc
     8041 ggcgccgccg ccgccggctc ccccgcccgc gccggccgag gcgcgcgagg cgcaggaagg
     8101 cagcccgcga tgaggagcct gctgatcgtc ggcgcggcgc tcgccggggt gctgatattc
     8161 ctgctggcga cggccagcgc ggcggattcg acgatgctgg cgcagcacta tccgctgctg
     8221 ctcggcctga acgcggcgct cgccgccgcg ctcgccgcgc tggtcgccta ccagttggcg
     8281 ggggtcgtgc ggcgctaccg cgggcgtgcg ttcggctcgc gcctgacgct gcgtttcctg
     8341 ctgctgttcg ccatgatggc ggtggtgccg ggcgtgctgg tctacacggt atcggtgcag
     8401 ttcctcacca agagcatcga atcctggttc gacgtcaagg tggacgcggc gctcgaaggc
     8461 ggcatcagcc tcggtcaatc ggccatcgac cgcatgctcg ccgagatgca gaccaagggc
     8521 cgcgccatgg cgctcgagct cgccgaacgc tcgccggcgc agcaggttgc gctgctcaac
     8581 cggctgcgcg agcaggctgg cgtgcaggag gcggtgctgg tcggcgccaa cggcacgctg
     8641 gtggcgagcg ccacggagga cgtcgcccgg ttcctgcccg aactgccgac ggcgcagatg
     8701 ctgcgccagg cgcgctcggc gcggggctac acctcgatcg acgccttgca gggcagaccg
     8761 ctggccctgc gcgtggtgct gccggtgacc ggcctggcga tcgccgacga atcgcgcttc
     8821 ctgcagctgc gccagatggt gcccgccggg ctggcgcgca gcgccgaggc ggtggaagcg
     8881 gcctaccgcg actaccgcga gcttgcgctg tcgcgccagg gcctgcggcg tatctacatc
     8941 gtcacgctga ccctggcgct gctgatgggc ctgttcgtgg cggtttcgct ggcggcaatc
     9001 ctgtcggcgc gcctgtcggc gccgctcgcc aacctggcgc aggcgacgca ggcggtggcg
     9061 cgcggcgact tttcgcagcg gccgccggtc accagccgcg acgaactcgg cgtgctcacc
     9121 gagtcgttca attccatgac gcgccagctc gacgaggcgc gtaaggtggt ggagagcaac
     9181 cgcatcgccc tggagacggc gaacgcctcg ctggagaata tcctcgccaa cctgtcggcc
     9241 ggcgtactgg tgttcgaccg cacgctgcgg ctgacgatct ccaaccgcgg cgccgcggag
     9301 attctcggca acgaactgca ggcccacgcc ggccggccgc tggccgcgtg gagcgcggcg
     9361 ctggagactt ttgccgccgg cgtgcgcgcc cagttcctgg agcacggcga gcgcacctgg
     9421 cagcaggaga tcagtttgcg cggcaccggc aagacgctgc tggcgcgcgg cgcggcgctc
     9481 ccggaggcga ccgggggcgg ctacgtgctg gtgttcgacg acatcacgca gctgatccag
     9541 gcgcagcgcg ctaccgcatg ggccgaggtg gcgcggcggc tcgcccacga gatcaagaac
     9601 ccgctcacgc cgatccagct ttccgccgaa cgcctggaga tgaagctcga gggccgcctc
     9661 gccccggacg acgcggaagt ggtgaagcgc agtacgcaaa ccatcgtcaa ccaggtcacg
     9721 gccctcaagt ccatggtgga cgatttcggc gactacgcga ggctgccggc gccggtgcct
     9781 gcgggcctcg atctgaatgc gctgatcacg gacgtgctgg cgctctatga aacctcgcgc
     9841 gtgccgatca ccgcgcggct ggcgcccggc ctgccgccgg tatgggccga taccgcgcag
     9901 atccgccagg tgatccacaa cctggtgcag aacgcccagg atgccctgga gaaccgcaag
     9961 gagccgcgcc tgctgccggc gatcgaggtg ggcaccgaac tggtcggcga caagctgcgc
    10021 ctgtcggtga ccgacaacgg cgacggcttt cccgaggaat tgatgacgcg catcttcgag
    10081 ccctatgtga ccaccaagcc gcgcggcact ggccttgggc tcgcgatcgt gaagaaaatc
    10141 gtcgacgagc accgcggcag catcgccatc gagaaccggc ccgacagcgg cgcgtcggtc
    10201 agcgtgctgc tgccgctggc ccaggcggcc tgagggcgcc atggcgcaga tcctggtggt
    10261 ggacgacgag atcggcatcc gcgagctgct ctcggaaatc ctggccgacg aggggcacgg
    10321 cgtgctgctc gccgacagcg ccggcgccgc gcggcggctg cgcggggcga accgcccgga
    10381 cctggtgctg ctcgatatct ggatgcccga taccgacggc atcacgctgc tcaaagaatg
    10441 ggccggcagc ggccagctga cgatgccggt ggtgatgatg tccggccacg gcaccatcga
    10501 gaccgcggtc gaggcaacgc gcatcggcgc gctcgactac ctggagaagc cgatcgccct
    10561 gcagcgcctg ctggcgacgg tgaagcgcgc gctgcgcaac caggagctcg ccgtgccgcg
    10621 ccagctgtcg atggcgatgc tcggccgctc ggcgtcgctc gccgaactgc gcaagcggct
    10681 ggcgcagctg gcacagacct cggcgccgct gctgctgcgc ggcgagcagg gcatgctgcc
    10741 cgaattgttc gcgcgccagc tcgcggcgcc cggcgcgccg ttcctggccg gcgccgaggt
    10801 gctggcggat gcctccagcg atctgctggc acgcgccggc ggcggcatcc tgtttctgcc
    10861 cgagctgtcg cgcctgacga ggggcgagca gaagaacgtc gagttcctgc aggcgcgcgc
    10921 cgaccggcac aagacgcgca tcgtgtcgtt ttccacgctc gacgcgaagg cgctggtcga
    10981 gcgctgcggc tacgatgccg ccctggtggc gcgcttgggc gagctgacgt tgcgcctgcc
    11041 gccgctgcgc gagttcgccg aggacgtgcc cgatcttgcc gcgcagatgc tggcgcagct
    11101 ggtcgaatcg cgcgcctgtc cgccgcggcg cttcgcggtg gcggcgctga acgcgctgcg
    11161 ccagcaccgt tggccgggga acctcgtcga attgcagcac gcggtgaaga acctcgccct
    11221 cagctcgctg gaggaggaag tgcgcgccga cgatgtcgag cgggtgctcg cggcgcagac
    11281 ggagcaggaa gcggccgccg aactggcgct cgaccggccc taccgcgagg cgcgcgaggc
    11341 gttcgagcgc gtgtacttcg agaacctgct ggccgcggag aacggcagca tgtcgcgcgt
    11401 cgccgagcgc agcggcctcg agcgcaccca cctgtaccgc aagctgaagg cgctcggctt
    11461 gccggtgggc cggcgcgagg aaggttgaaa gagcctagaa tgccccgctt tgcgcaagga
    11521 gcaggcgcgg cgtgaagatc gtcatcctcg gagccgggca ggtcggcagc tcggtcgcgg
    11581 agagcctggt ctccgagcag aacgacatca ccgtggtcga cgtcgagccg gggcgcctgc
    11641 gcgccctgca ggaccggctc gacctgcgca ccgtcatcgg cagcgcctcg cacccatcgg
    11701 tgctcattga ggcgggcatc gaggatgccg acctgctgat cgcggtgacg caatccgacg
    11761 agaccaacct ggtggcctgc aagctggcgg cgcgcatgtt caacgtgccg cggcgcatcg
    11821 cgcgcatccg ggccaacgcc ttcctc
//
LOCUS       DASRIE010000195        32260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2809517, whole genome shotgun sequence.
ACCESSION   DASRIE010000195 DASRIE010000000
VERSION     DASRIE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809517"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="ispH"
                     /locus_tag="VFB53_08085"
     CDS             complement(<1..599)
                     /gene="ispH"
                     /locus_tag="VFB53_08085"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZN86727.1"
                     /translation="MDVLLANPRGFCAGVERAIDIVERALEERGAPIYVRHEIVHNKS
                     VVEDLRKKGAVFVEELDEVPAGATVIFSAHGVAKTVRSDAEARGLKVFDATCPLVTKV
                     HVEVSKMLREGYQIVMIGHRGHPEAEGTMGQSASGMHLVETLEDVARLAVQEGARLAY
                     VTQTTLSVDDTQVIVAALKARFPQIRGPKKDDICYATQNR"
     gene            complement(610..1074)
                     /gene="lspA"
                     /locus_tag="VFB53_08090"
     CDS             complement(610..1074)
                     /gene="lspA"
                     /locus_tag="VFB53_08090"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZN86728.1"
                     /translation="MPKAAPWFLVAALVLAADRLTKWLILGWLRPGESYEVTGFLELV
                     LVFNRGAAFSFLADAPGWQRPFFIAVAVVVSAIVCWMLWRHPGRRLLGAGLALILGGA
                     LGNLWDRVAIGAVVDFLDFHAYGWHWPAFNVADSAISAGAALVIADGFRHRG"
     gene            complement(1067..3841)
                     /gene="ileS"
                     /locus_tag="VFB53_08095"
     CDS             complement(1067..3841)
                     /gene="ileS"
                     /locus_tag="VFB53_08095"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZN86729.1"
                     /translation="MPDYKSTLNLPDTPFAMRGDLARREPLQVKQWQDQGVYRRLRAA
                     ARGRPTFVLHDGPPYANGDIHIGTAVNKILKDMVVKSKLLAGFDAPYVPGWDCHGMPI
                     EVQIEKQHGKNLPVEQTLKLCRAFATEQIANQKKDFQRLGVLGDWDHPYLMMAPGNEA
                     DEIRVLGKLLEKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDPAIDVGFPFVENEK
                     LAKAFNARIDKPGFIVVWTTTPWTIPANQALNVHPEVTYHLVDTVKGLLILAADLQEA
                     CLKRINLQGRAIAACKGRALENIRFRHPFYERESPVYLGEYVTLDAGTGIVHSSPAYG
                     VEDFESCRRNGMQDGEILTPVMGDGKYAPSLPLFGGLSIWEANPKVVQAIEARGALFA
                     VDWKFTHSYMHCWRHKTPIILRATTQWFAAMDKPVNGESLRATALRGIGNTQFFPSWG
                     QARLHGMIANRPDWTLSRQRQWGVPMPFFLHKETGALHPRTPELLEVVAKRVEKGGIE
                     AWQSITAEELLGAESSSYEKSKDTLDVWFDSGSTHFTVLRNSHKAGSAYPADMYLEGS
                     DQHRGWFHSSLLTACMLDGHPPYRSLLTHGFVVAGDGRKMSKSLGNTMAPQKISGTLG
                     AEILRLWVASTDYSGELSISDEILKRVVESYRRIRNTLRFLLANTSDFDVAQDAVPVA
                     QMVEIDRYALALAARLQEQVRRDYAAYQFHLVAQKILAFCAEDLGAFYLDILKDRLYT
                     CGARSAARRSAQTALWHVAQALARIMAPILSFTAEEAWQVMAGNADQSVFELVFHDIP
                     DAGLDDAAMRRWEDIRQLRELAAKKIEEQREAKQIGSSLAAELDIRAGDPLYASLASL
                     GEDLKYVFITSRASIQRAQGAATTVAVQPSRHPKCERCWHYRADVDAAGLCGRCEGNL
                     KGPGERRTHA"
     gene            complement(3834..4730)
                     /locus_tag="VFB53_08100"
     CDS             complement(3834..4730)
                     /locus_tag="VFB53_08100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZN86730.1"
                     /translation="MALTIGNFDGVHLGHQAMLERTVARARELRVPACVLTFEPHPRE
                     FFAPATAPARLTRLREKLELMARAGVGRVHVARFDARLAALAAERFVEQIVAQGLRAA
                     WLLVGRDFRFGARRTGDYALLGALAARHGFELEAMPDVALQGARVSSSAVRAALAAGD
                     LRAAERLLGRPYAMSGRVAHGEKLGRELGFPTANIVLRRRPALGGIFVVECRVEDEAA
                     WRPGVASVGRRPTVNPTALPLLEVHLLDFAGELYRRHLQVRFVAKLRDEEKYDDLGAL
                     RAAIARDAAQARSHFAKKKTNA"
     gene            4822..5358
                     /locus_tag="VFB53_08105"
     CDS             4822..5358
                     /locus_tag="VFB53_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZN86731.1"
                     /translation="MQTLEQQMSFYAAYHQDARNKASHFVGVPMIIFGLFIALGWARV
                     EIGGVPVTAAMLLAAAVLVWYFLLDVPLALAMLAVNAALLVLADMVSVQPIGAGLLWF
                     LAFFGGGWVIQLVGHVWEGRKPALVDNFFQIFVAPIFLAAEVFFALGYKPALHAAVQK
                     RALEMRAAAGVAERTAAA"
     gene            complement(5363..6214)
                     /locus_tag="VFB53_08110"
     CDS             complement(5363..6214)
                     /locus_tag="VFB53_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN86732.1"
                     /translation="MERLHPFAELMAREEAGIDLGRACLLIARDAYPGLDVEGYLGEI
                     ERLAARLRGRLPRDGSAEARVVALNEFLFDDLGYSGNADDYYDPRNSYLNEVLDRRTG
                     IPITLSVLYLEIGRRVGLTLEGVSFPGHFLVSLRLRGATLVLDPFSGGAPQSEADLRE
                     RLARVIPENATGNVPVAQLPLDQFLEPAGKRQILARLLRNLKGIYRNTDQPQRLLEVL
                     NRMLEVAPAAHGERRERGLLYQRLECYRAALKDLQDYSTLAPEAPDIDEVRARLVELG
                     ALCARLN"
     gene            complement(6214..7755)
                     /gene="murJ"
                     /locus_tag="VFB53_08115"
     CDS             complement(6214..7755)
                     /gene="murJ"
                     /locus_tag="VFB53_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZN86733.1"
                     /translation="MNLLRALATVSSMTFLSRVLGYVRDFFIARLFGAGLAADAFFVA
                     FRIPNLLRRMFAEGAFSQAFVPILSETRNRQTPEETRSLVDAIGTLLFLALLAATALG
                     MLAAPLIGYLFAPGWFHAEPEKFGLTVQMLRITFPYIFFISLVALAAGVLNTWSRFAV
                     PAFTPVLLNVSFIVAAAFFAERFDPPVLALAWAVFVGGALQLALQLPFLARIGMLPRW
                     RLDWRHPGVQRVLKLMLPALFGVSVAQISLLINTIFASFLASGSVSWLYYADRLMEFP
                     AGVLGVAVGTILLPSLSRHHAGGDGARYGQLLDWGLRVTLLLALPAAAALAVLAVPLI
                     ATLFHYGRFTAEDVWMTRQALMAYSLGLVGMILVKILAPGFYARQNVATPVKIGLATL
                     AATQLMNLAFIVPFKHAGLALAIGLGACLNAALLYRGLRKAGIYAPQPGWILFTLKIT
                     LAVAAMAAALHFAMGAAAWWLQAPWQDKLLRIAVLVPGGALGYGLLLLAMGFRLRDFA
                     RREAG"
     gene            7794..8057
                     /gene="rpsT"
                     /locus_tag="VFB53_08120"
     CDS             7794..8057
                     /gene="rpsT"
                     /locus_tag="VFB53_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZN86734.1"
                     /translation="MANIKSSRKSARQAIVRRAHNFTLRTEVRSAIKRVKKAIAAGDK
                     AAAAAALEKSRSVIDRVAAKGVLHRNAAARHKSRLAQSIKAMQ"
     gene            complement(8062..8391)
                     /locus_tag="VFB53_08125"
     CDS             complement(8062..8391)
                     /locus_tag="VFB53_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HZN86735.1"
                     /translation="MPEPAIVEFVIQGVTLDGKPFRPSDWAERLCGVMAAFGSDQRMQ
                     YSPFVHPVNAGGVRCVVVDTRLEAVEPMAYRFLLSFAKDNELRTRDGRVPERTELAKL
                     QRAGDTD"
     gene            8415..10388
                     /locus_tag="VFB53_08130"
     CDS             8415..10388
                     /locus_tag="VFB53_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN86736.1"
                     /translation="MAKPHSRILVVNDEHLVLREFVKGLNAAARSLDNPLGISFAGVT
                     TAKEALAAIESDGDIQAVLVDDRLYTLGKHNGSRKLQVSALEFVQRITRFRPELDVYI
                     LIAQEKEDDVVDALFTESVDGYFYREERDYRGIYRILNAQIQERARTPFTDQLKNYVW
                     MAKDQWHTPGHSSGESLRGSPWVNDFYEFMGEHVFDADLSVSVPMLDSLMEPKGVIAE
                     AQAMAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLLLDRNCHKSVHHGVVLSGA
                     HPVYLDSALNRKYGLYGPVPKKTLLAAIRNHPDAQALILTSCTYDGLRYDLAPIVAAA
                     HAKGIKVIVDEAWYGFARFHPEFRPTALEAGADYATQSTHKVLSAFSQASMIHVNDPG
                     FREHLFRENFNMHTSTSPQYGLIASLDVARKQAVMEGYKLLSRTLALGKELRAQINST
                     GVFRVLELEDLLPDEVKQDGIRLDPTKVTIDISGCGTTVDELQKELFERYNVQTEKST
                     FNTLTLLLTIGTTRSKVSRLYDALMRIARERRAPRRLVRTPEVPHFTRLRYLPRDAYY
                     CGGELVPVFDDKEGVNRALARRICADQIVPYPPGIPVLVPGQAITREIAEYLASLLRS
                     QKRMELHGIVMEGYQPCVRVLRSAEERSLHRIG"
     gene            10446..11651
                     /locus_tag="VFB53_08135"
     CDS             10446..11651
                     /locus_tag="VFB53_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN86737.1"
                     /translation="MSHVMNTYARQPVAFVRGQGVWLYDEAGKQYLDALAGIAVNTLG
                     HNHPRLVKALTEQIGRIIHSSNLFQVPLQEQAADRIAQITGLDEVFFCNSGLEANEAA
                     IKVARKYGHDRGVAEPAIIVMEKAFHGRSLATLSASGSRKVQAGFEPLVQGFVRVPLN
                     DLDAVRQVAANNRNVVAVFIEPIQGEGGINVSRLEYLRGLKEICDANQWLFMSDEVQC
                     GLGRTGKWFVYQHAGFQPDVVPLAKGLAGGVPVGCCVVGNRAKGVFKPGNHGSTFGGN
                     PLAMCGVVTTLDTVRDEGLLENAARVGEAIRGGLAAALAGVAGVVEVRGMGLMLGVEL
                     DRPCGELVKMGLDAGLVCNVTADNVIRLLPALVMSDVEGRQVVERLVPLVKEFLARSG
                     AQPRLAAAR"
     gene            11671..12594
                     /gene="argF"
                     /locus_tag="VFB53_08140"
     CDS             11671..12594
                     /gene="argF"
                     /locus_tag="VFB53_08140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZN86738.1"
                     /translation="MAKLRHFLQFNDFSRAEFERLFARTRWIKDQFKRYKRYWPLEDR
                     TLVMIFEKQSTRTRLSFEAGMHQLGGAAIFLNTRDSQLGRGEPVEDAAQVISRMCDVV
                     MIRTFEQEIIERFAANSRVPVINGLTNEYHPCQVLADIYTYVEQRGAIKGRTVAWIGD
                     SNNVCNTWLQAAEVFDFNVHVSTPPGYEVEPERANLYGTSHYEEFRDPMQAAKGAHLV
                     TTDVWTSMGFEAENEARKRDFEDWQVDAEMMRAARKDALFMHCLPAHRGEEVAAAVID
                     GKQSVVWEEAENRLHTQKALLEFLVLGKVEK"
     gene            12594..13205
                     /locus_tag="VFB53_08145"
     CDS             12594..13205
                     /locus_tag="VFB53_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZN86739.1"
                     /translation="MFGVADYWAFVVAVVVFLAIPGVGNLAIITSTTKGRIAGGLAAT
                     LGVIVADQLLMWLAVAGVAAVLTAHPAVFGVVQYAGALYLAYLGIRMILAKPGDAPVL
                     DMAPRRFFRQAMLITLLNPKAIVFYMAFFPLFVDPARHQGLLTFGVMALTIAVLTFLY
                     CLIVIAITHFAADRMRASPRTAVWLNRVAGTMLVGFGVKLSVS"
     gene            complement(13192..14529)
                     /gene="argG"
                     /locus_tag="VFB53_08150"
     CDS             complement(13192..14529)
                     /gene="argG"
                     /locus_tag="VFB53_08150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZN86740.1"
                     /translation="MATILGTLPKGEKVGIAFSGGLDTSAAVHWMRAKGAIPYAYTAN
                     LGQPDEPDCAAIPRQALAYGAEKARLVECRPQLVAEGIAALQCGAFHISTGGATYFNT
                     TPLGRAVAGTLLVVAMREDAVGIWSDGSTFKGNDIERFYRYGLLANPALRIYKPWLDQ
                     RFIDELGGRKEMSAYMAKAGFDYKMRAEKAYSTDSNILGATHEAKDLEFLDKGLSIVQ
                     PIMGVASWRAEVAVKPEKVAVRFEEGVPVALNGQQTRDAVALLAEANAIGGRHGLGMC
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRSNGRRLGRLLYQG
                     RWFDPQTMMLRETAQRWVAKAVTGEVTLELRRGNDWSILDTRSPNLSYKPERLTMEKG
                     DEVFFTPADRIGQLTMRNLDIADTRDKLRVYGEAGLLGDGGVPLLGAEKPKKR"
     gene            14568..15230
                     /locus_tag="VFB53_08155"
     CDS             14568..15230
                     /locus_tag="VFB53_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86741.1"
                     /translation="MTIVRALAFPLLAALSLSCAAAPFAVRLGTERVALDAPPGFADA
                     LELGSPRLQELAESLTSASNRILLFALTDADLRRFTLGDPPDLRRYLLVATPRAMERD
                     RVGLAQFRSYAADATRGLGNPARPADLRRHLDAQPPGRPSMIAELRRTPQAITLLVGS
                     RLAGPDGKNVYLLSTTTLLLVQAKTLSLAAYTGYDDDADLDWLRDTTLRWVDELQRLN
                     SR"
     gene            15202..17400
                     /locus_tag="VFB53_08160"
     CDS             15202..17400
                     /locus_tag="VFB53_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZN86742.1"
                     /translation="MSSSASIPADPAELSGVGPYAGRVADAYRAPDLLAALAGLPALL
                     AAPAARLVATGRNRNLRVELPVHGRRVAVMVKAFGAQSWLRDLRDLRRGSKARRTWQA
                     ATHLAERGVGTPPPIAFLERWEGARLRESYYLAEFQDGATTFREALLELFERRPPLAA
                     QFVQLLECVAAGVRRMHDAGFLHNDLGNQNVLLRPGGDAAWRDFMLVDLNRGRVRPAL
                     GLRQRGRDLSRLELPSDLLQILVEMYWRGTPPRALLGTARLYRRLYGLQVLKRRLLQV
                     LRGGAREAPGRDYPAPRELWIWDEPTAQPISALLRRDRARLFPLSRYRRVLVDGLRAA
                     PGVWREYRALRRAAYAHPVQFAGRIGMALEPTPATREQELALLAGLGRIPALVRFYHH
                     DDAARRAFRVELVHALHRAGHPVAAALVQDRRALREPASWRAFAREVLERIAPVVQEV
                     EVGHAINRVKWGVWGFEELRALYAPLPDLQSRFPALRFMGPAAIDFEYPAVFPALREW
                     PRAVPLAALSHHLYVDRRGAPENPQDGFGAPEKFALGRAAARASGAARFIVSEVNWPL
                     HGMGAQSPIHAPYFPPWRQASDPGVGEEQYGDYLLRYLCLALGSGMVERVYWWRLAAR
                     GYGLADDAQPAALRPRPAYFMLRHFLALLGDATLLSARLPARRGERHGRYRFAFRRAD
                     GEIVALTYAHGPGLPFPADDACARVEDAFGETAAPAGLGGRPVYLRGARA"
     gene            17410..17895
                     /locus_tag="VFB53_08165"
     CDS             17410..17895
                     /locus_tag="VFB53_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZN86743.1"
                     /translation="MPSFDIVSQVDQQEVRNAVEQANKEIQNRYDFKGSDARIEQDGL
                     ALTVFADDEFKLGQVMDVLRQRMAKRGIDVRSLELGAVEKISGDKAKRPVTVKTGIAQ
                     EKAKQIQKLVKDARLKVQPSIQGDLVRISGAKKDELQAAIQLVRQQVADLPLQFINFR
                     D"
     gene            complement(17879..18592)
                     /locus_tag="VFB53_08170"
     CDS             complement(17879..18592)
                     /locus_tag="VFB53_08170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZN86744.1"
                     /translation="MKPVAIFRHAPSEGPGYFATALERKSIPWTLVALDEGRGVPRDA
                     RRYAGLAFMGGPMSANDDLPWIGPALALIGDAVRKDVPVLGHCLGGQLMARALGGEVR
                     AAPVTEIGWGEVRACANGVAREWLGGLRAFEAFHWHGETFSIPPGGTRVLENAHCDNQ
                     AFALGKHFGMQCHVEMTAELIGDWLQGGAREIEASRASAGVQQPGQVRKDMERRLTAL
                     HAVADRIYERWCEGLSRGS"
     gene            18576..19226
                     /locus_tag="VFB53_08175"
     CDS             18576..19226
                     /locus_tag="VFB53_08175"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZN86745.1"
                     /translation="MATGFMTRHCSALVLALAALPALALDVSLIGTFGDKAAVLVIDG
                     GAPKTVKVGQRAGGVTLVSVERDRAVIEADGGRRTLARGQHYISGAAGDQRQSVTLAA
                     DSRGHFFSDGAVNGGAVRFVVDTGATLIALPASDAVRLGLDYRSGKLALTKTAGGLVP
                     VYRVKLDSVRLGGIELAAVEGIVIEQGLDIALLGMSFLNRVEMRHEGRVMTLTRRF"
     gene            complement(19234..19989)
                     /locus_tag="VFB53_08180"
     CDS             complement(19234..19989)
                     /locus_tag="VFB53_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN86746.1"
                     /translation="MRESGEALVETFGITKGLKVLDLGCGDGTTAIPAAKRGADVLGV
                     DIAANLVAAGNKRAKDLGLANCRFQEGDASDMKGVPDRSFDLTVTIFGAMFAPKPFDV
                     AKEMVRVTRPGGRIIMGNWIPNDPTLVAQILKISSAFTPPPPDGFVSPMTWGIEANVT
                     ERFAAAGVPAKKISFVRDTFTFNLSGTPEDFVGLFRKYYGPTMNAFDAAEKNGRATDL
                     QRQLEDLVQAQNKSTRKDKTSIPATFLRVTVAL"
     gene            complement(20194..20874)
                     /locus_tag="VFB53_08185"
     CDS             complement(20194..20874)
                     /locus_tag="VFB53_08185"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZN86747.1"
                     /translation="MSAADAQDHAAQRARLVAEVEAMVAATRRETGIAALSAPVRAAL
                     GKLERHRYVPADRQDLAYGNHPVPIGAGQTISQPYIVALSTELIAPRPGQRVLEIGTG
                     SGYQAAMLAEIVSTVYSIELIPALGKEAAQRLRTLGYSNVEVRIGDGYAGWPEQAPFD
                     GIVVTAAAPQVPQALVDQLRPGGRMVIPVGASLAAQELLLLEKRADGSVERRSVLPVR
                     FVPLVPGK"
     gene            complement(20897..21700)
                     /gene="folE2"
                     /locus_tag="VFB53_08190"
     CDS             complement(20897..21700)
                     /gene="folE2"
                     /locus_tag="VFB53_08190"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZN86748.1"
                     /translation="MNAKDPHHIPDIQSLPDARKLAIDKVGIKAIRHPVQVMQRAGGV
                     QHTIANFNMYVGLPHQFKGTHMSRFVEILNAHERELTVASFNLMLREMVDKLEAKEGR
                     IEMAFPYFIDKAAPVSGVRSLMDYEVTFVAQILKGEQCFAMKVLVPVTSLCPCSKKIS
                     DYGAHNQRSHVTVTAHTGDFVWIEEIVDLVEKQASSELYGLLKRPDEKYVTERAYDNP
                     KFVEDMVRDVAAALNRDKRITRYVVESENFESIHNHSAYALIERDKKAG"
     gene            complement(21726..23564)
                     /gene="dxs"
                     /locus_tag="VFB53_08195"
     CDS             complement(21726..23564)
                     /gene="dxs"
                     /locus_tag="VFB53_08195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZN86749.1"
                     /translation="MFELLETIDDPAALRKLERRQLHQLAEELRAFVLDSVSKTGGHL
                     SSNLGTVELTIALHAVFDTPEDRIVWDVGHQTYAHKILTGRRAAMARLRMQDGISGFP
                     RRAESPYDTFGTAHSSTSISAALGMAVAAKAKGEARKCIAVIGDGAMSAGMAFEAMNN
                     AGAMDADLLVVLNDNEMSISPPVGALTNYLARLLSGRMYSTARRASEHVLKRVGVWDL
                     ARRTEEHLKGMVTPSTLFEEFGFNYIGPIDGHDLDTLIATLANIKQLKGPQFLHIVTR
                     KGQGYKLAEDDPVAYHGPGKFDPAQGLQKAAPGKPTYSQVFGDWLCDMARADERLVAV
                     TPAMREGSGMVRYSEEFPARYFDVGIAEQHAVTFAAGLACEGLRPVVAIYSTFLQRGY
                     DQLIHDVAIQNLPVSFALDRGGLVGGDGATHNGAFDYAYLRAVPNLTVMAPSDANECR
                     QMLTTAFRLGKPAAVRYPRGVAPGEAGKDLSDLPVGKGELRRRGRRVAILAFGAMLKP
                     ALEAAEALDATVANMRFVKPLDVELVRQLAQDHALLVTVEEHQVMGGAGSAVCEALNQ
                     LGIERRVLLLGLPDRFVDHGEPGKLLSSVGLDAPGIRQAIETVIAE"
     gene            complement(23567..24415)
                     /locus_tag="VFB53_08200"
     CDS             complement(23567..24415)
                     /locus_tag="VFB53_08200"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZN86750.1"
                     /translation="MEAALARLLPGAQIAPARLHEAMRYATLEGGKRVRPLLVFAAGE
                     VAQARPERLEVAAAAVELIHAYSLVHDDLPCMDDDVLRRGKPTAHVEFDEATALLAGD
                     ALQSLAFQLLAEHPLADDARAQLEMVRLLAAAAGSRGMAGGQQIDLESTGKQLEPPAL
                     ESMHIHKTGALIRAAVLLGVCCAGAPAADLRARLDQYAKYVGLAFQVVDDLLDHEAST
                     ATLGKTSGKDARQGKSTYVSVMGLPRARELAEELRQNALRALEPLGAGAQRLRGLADF
                     IVLRKF"
     gene            complement(24456..24698)
                     /locus_tag="VFB53_08205"
     CDS             complement(24456..24698)
                     /locus_tag="VFB53_08205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZN86751.1"
                     /translation="MAKTAQEPGFEKALAELEKLVAQMEGGKLSLEQALEAHKRGLEL
                     ARYCQERLQAAQRQVQVLEGEVLKRLAADAEPGERA"
     gene            24785..25882
                     /locus_tag="VFB53_08210"
     CDS             24785..25882
                     /locus_tag="VFB53_08210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN86752.1"
                     /translation="MSDLPPLRQLKPATCQLPVSWYFDPQIFAAEKKLLFDAGANYVG
                     HELWVPQPGDYRTLEWLDHAKVLVRNDTGVELLSNVCRHRQSIMLEGRGRTENIVCPL
                     HRWTYDLRGELLGAPRFAEQPCVRLHSTPLSNWQGLLFAGPADPRQALAGMRNLGDWD
                     FSGYQLDRVMVEEYNFNWKTFIETYLEVYHVDFFHPGLGNFTDCDNFAVDYGRDWSVQ
                     IVPAKAGLQKPGTPAYRKWQDACLQQLSGRTPKQGALWMTYFPGLMLEWYPNVLVVSR
                     LVPRSPTRTTNVVEFYYPEEIVLFEREFIEAQQAAYVETAIEDDEICLRMDRGRRALW
                     EEGRDDAGPYQSPMEDTMVHFHEWVRRGLGR"
     gene            25855..26703
                     /locus_tag="VFB53_08215"
     CDS             25855..26703
                     /locus_tag="VFB53_08215"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZN86753.1"
                     /translation="MGAAGPRALNTLVATGELARHPEWRVFDCRHDLFKHDLGEQQYR
                     ASHIPGAAFAHLDRDLSAPKNGANGRHPLPDPNVFIAWLGRQGLKPADQVVAYDGGPG
                     AMAARLWWMLRWVGHDKVAVLDGGFAKWVQEGRPVTADIPAFPPTAYPGKVRADRAVD
                     VTFVQQHLGEISLLDGRAPARWRGETEPIDPVAGRIPGSLNRFSSDNLTAEGTFKQPE
                     VLKHEFLSVLGTRKLEAIVNSCGSGVAACHNLLAMEIAGLKGARLYAGSWSEWIADPA
                     RPRERG"
     gene            26690..27052
                     /locus_tag="VFB53_08220"
     CDS             26690..27052
                     /locus_tag="VFB53_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HZN86754.1"
                     /translation="MSAADALLVVHFAIVAFIAGGLVLTWVGAVLGWGWVRNRWFRYV
                     HLAAIAFVAVEALIGMTCPLTEWEDALRGGARPESFIGRWVQQLLYYRAPEWVFTTLY
                     LAWAAATLLTLRLVPPAR"
     gene            27086..28123
                     /gene="dctP"
                     /locus_tag="VFB53_08225"
     CDS             27086..28123
                     /gene="dctP"
                     /locus_tag="VFB53_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZN86755.1"
                     /translation="MNKPLRMRTAAPVLAAAALLAAPALAQTVDGPKVDWKLATWGKP
                     RAFTAGIETLAKHVGERTGGKFTITIGYSTFGGEKEILDILNSGGLQMTTICSSYHPD
                     KHPAYTGLDLPFLPLPDFDTQWAVHDAYHAHPYIQKELARWNAMPYSSNLLPQYEFIG
                     KGKAPRSLADFKGMRVRAIGGIGDAMKNLGAVPTSVPATEVYTSIERGTVEAASFPST
                     YAHAAYKIHEVGSWYTTNLSPGTQACPTLINRNAWAQIPAQYQKLFEEARPRVKQALT
                     KAYGAADEKNLPLFKRRLAFVTYTPAELAQFHQVGAKPVWDAWVAEMSAKGVPARELL
                     DLIFASAKARK"
     gene            28150..28839
                     /locus_tag="VFB53_08230"
     CDS             28150..28839
                     /locus_tag="VFB53_08230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZN86756.1"
                     /translation="MAGPSASADHAPRPPAGAPLLARADWRLARLENGLNLVAAAAIF
                     GVMAFSVAQIVSRSLSSWLHALIPAIPQIAIHGYIDYIEFIAVLYAVLGIAYCQRLGG
                     HIRMEIVVANFRGRALWMMEALAVLLAVAVTVLLIAGTGDNFLNAWTKGDSSMDIKLQ
                     LWPSKLVVPLMLGVLLARLLLQLWAYLRLAADPSLAPIAVPVPETPEQAARREIEEAI
                     GKLGPDDAPRG"
     gene            28844..30148
                     /locus_tag="VFB53_08235"
     CDS             28844..30148
                     /locus_tag="VFB53_08235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZN86757.1"
                     /translation="MDPLTIGICGAAALVVLAVLGVRIAYAAALVGTLGLMILMRDPY
                     AALVTAGTVPYAKGTSYELSVLPMFILIGYLAYYAGMTTTSFEAAKRWFGWLPGGLAI
                     GTIFAVAAFSAVSGASTAAAAVFARVAIPEMLKAGYNRALAAGVVAAGSTLDSLIPPS
                     ALLVIYGIIVEQSIGDLLIAGFVPGILSALVYCAIVVLRCRLDPALGRPIRGYTWTQR
                     WQSLPPTAPIWIVVVLIFTAMLFGWATPTEAGALGAFSTLLFAWRHGMSWGQFRGAML
                     DAAKLSVMIFTIIWGVLIFVRFLGYANLPLAFAGWIQSLALHPLLVLLAIYFIYLVLG
                     CFMDAIGMMLLTLPIVFPVIEGLGYDPIWFGIIVVKLCGIGLLTPPVGLCAFVVNGVR
                     PDIPLGDVFRGIWPFVLADVATVALLTAYPELVTFVLTLKPR"
     gene            complement(30145..31497)
                     /locus_tag="VFB53_08240"
     CDS             complement(30145..31497)
                     /locus_tag="VFB53_08240"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZN86758.1"
                     /translation="MLKHTRWLAVLAVFLAACAPVPVAQQQQPKNIILLFADGTAATQ
                     WELGRYSSRALRKSGFAVTDVVFHQGSLGFLTTHPYEAFATDSASAASAMSTGVKTTI
                     GAIAVGPDGKPARTAAEVARAQGKRIGLVTTAEIYDASPAAFSVHARSRRDAASIVDQ
                     YLALAPDVLMGGGADQFKPAHLEGFRGKGYALARNAAELKAAAGPRLLGLFAEESMAY
                     AIDRDAGEPSFADMMRAALKALATDNPRGFFLFIENENTDTAGHRNDAAALMRDLWAF
                     DEALRVALEFQRGSPDTLIIVTGDHETGGLSATYAFRDLSSTSSKNRFYTGDAQLQLL
                     AGISISFDKALEQLGKKPTNEGIDKLLAAHFPGFRLDADLREAILKGQILERNNSYPV
                     RNALGRMVARRTGFYWGTSGHSTEPVVVGAIGPGAERFRGYHDNTDFGRILHGLLGQR
                     "
     gene            complement(31523..>32260)
                     /locus_tag="VFB53_08245"
     CDS             complement(31523..>32260)
                     /locus_tag="VFB53_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZN86759.1"
                     /translation="AELQTELDAMAKERTTQRARRNERQDLARVALVGYTNAGKSTLM
                     RALTGNAALVADKLFATLDTTVRALQPESVPRILVSDTVGFIKNLPHGLVASFKSTLD
                     EALEASLLAHVVDASDAGFERQLAVTEEVLDEIGAKGVPRLLVFNKIDRGGDAAALRD
                     RYPGCVVMSARRAADVAALREAIVAFFRRDLVEAELFLPWAAQKLRGEIFAACEVLEE
                     RADGDGVLLRVRGAPEALQRLRAVHRR"
BASE COUNT         4740 a        11505 c        11002 g         5013 t
ORIGIN      
        1 cggttctgcg tggcgtagca gatatcgtcc ttcttcggcc cgcggatctg cggaaaacgc
       61 gccttgagcg ccgccacgat cacctgcgtg tcgtccaccg acagcgtggt ctgcgtgacg
      121 taggccagcc tggcgccctc ctgcaccgcc agcctggcca cgtcctcgag cgtctcgacc
      181 aggtgcatgc cgctcgccga ctgccccatg gtgccttcgg cttccgggtg cccgcgatgg
      241 ccgatcatca cgatctggta gccctcgcgc agcatcttcg acacctcgac gtgcaccttg
      301 gtgaccagcg ggcaggtggc gtcgaatacc ttgaggccgc gcgcctcggc gtcgctgcgc
      361 accgtcttgg cgacgccgtg cgcgctgaag atgacggtgg cgccggcggg cacctcgtcg
      421 agctcctcga cgaacaccgc gcccttcttg cgcagatcct cgaccacgga cttgttgtgc
      481 acgatctcgt ggcgcacgta gatcggcgcg ccgcgctcct cgagcgcgcg ctcgacgatg
      541 tcgatggcgc gctcgacgcc ggcgcagaag ccgcgcggat tggcgagcag cacgtccatg
      601 gcggccattc tatcccctgt gccggaagcc gtcggcgatc accagggccg cgccggcgct
      661 gatggcggag tcggcgacgt tgaacgccgg ccagtgccag ccgtaggcgt ggaagtcgag
      721 gaaatccacc accgcgccga tggcgacgcg gtcccacagg ttgcccagcg ccccgcccag
      781 gatcagcgcc agcccggcgc ccagcagccg cctgccgggg tggcgccaga gcatccagca
      841 gacgatggcg gacaccacca ccgccaccgc gatgaagaag ggacgctgcc atcccggcgc
      901 gtcggcgaga aagctgaagg ccgcgccgcg gttgaacacc agcaccagct cgaggaagcc
      961 ggtcacttcg taggactcgc ccggccgcag ccagccgagg atcagccatt tcgtcagccg
     1021 gtcggccgcc agcaccagcg ccgccaccag gaaccagggc gccgccttag gcatgcgtgc
     1081 gccgctcgcc ggggcccttc aggttgcctt cgcagcggcc gcaaagccct gccgcgtcca
     1141 cgtccgcacg gtaatgccag cagcgctcgc acttcgggtg tctgctgggc tggacggcga
     1201 ccgtggtcgc cgctccctgc gcgcgctgga tcgaggcccg cgaggtgatg aacacgtact
     1261 tcaggtcctc gcccaggctg gcgagcgatg cgtagagcgg atcgccggcg cggatatcga
     1321 gttccgccgc cagcgaagaa ccgatctgct tcgcttcccg ctgctcctcg atcttcttgg
     1381 cggcgagttc ccgcagctgc cggatgtctt cccaccggcg catcgccgca tcgtccagcc
     1441 ctgcatcggg gatgtcgtgg aacaccagct cgaagacgct ctggtccgcg tttcccgcca
     1501 tcacctgcca ggcttcctcc gcggtgaagc tgaggatcgg cgccatgatg cgcgccagcg
     1561 cctgcgcgac gtgccacagc gcggtctgcg ccgagcgccg cgccgccgac ctggcgccgc
     1621 aggtgtacag gcggtccttc aggatgtcga ggtagaacgc gcccaggtcc tcggcgcaga
     1681 aggccaggat tttctgcgcc accaggtgaa actggtaggc cgcatagtcc ctgcgcacct
     1741 gctcctgcag gcgcgccgcc agcgccagcg cgtagcggtc gatctcgacc atctgcgcga
     1801 ccggcaccgc atcctgcgcg acgtcgaagt ccgaggtatt ggcgagcagg aagcgcaggg
     1861 tgttgcggat gcgccggtag ctctccacca cgcgcttgag gatctcgtcg gagatggaaa
     1921 gctcgcccga gtagtcggtg ctggcgaccc acaggcgcag gatctcggcg cccagcgtgc
     1981 cggagatctt ctgcggcgcc atggtgttgc cgagcgactt cgacatcttg cggccgtctc
     2041 ccgccaccac gaagccatgg gtcagcaggc tgcggtacgg cgggtggccg tccagcatgc
     2101 aggcggtgag cagcgacgag tggaaccagc cgcggtgctg gtcggatccc tcgagataca
     2161 tgtccgccgg ataggccgac cccgccttgt gcgagttgcg cagcacggtg aagtgcgtgc
     2221 tgcccgagtc gaaccacacg tcgagggtgt ccttgctctt ctcatacgaa gaactctcgg
     2281 cgccgagcag ttcttcggcg gtgatgctct gccaggcctc gatgccgccc ttttccaccc
     2341 gcttcgccac cacctcgagg agctcggggg tgcgcggatg cagcgccccg gtctccttgt
     2401 gcaggaagaa cggcatcggc acgccccact ggcgctgccg cgacagcgtc cagtcggggc
     2461 ggttggcgat catgccgtgc aggcgcgcct ggccccagga cgggaagaac tgcgtgttgc
     2521 cgatgccgcg cagggccgtg gcgcggagcg attctccgtt cacgggcttg tccatcgcgg
     2581 cgaaccactg cgtggtcgcg cgcaggatga tcggcgtctt gtggcgccag caatgcatgt
     2641 agctgtgggt gaacttccag tccacggcga acagcgcgcc ccgcgcctcg atcgcctgca
     2701 ccaccttcgg gttcgcctcc cagatcgaca acccgccgaa cagcggcagc gacggcgcgt
     2761 acttgccgtc gcccatgacg ggcgtgagga tttcgccgtc ctgcatgccg ttgcggcggc
     2821 aggactcgaa gtcctcgacg ccgtaggccg gcgacgaatg cacgatgccg gtgccggcgt
     2881 cgagcgtgac gtactccccg aggtagaccg gcgactcgcg ctcgtagaac ggatgcctga
     2941 aacggatgtt ttccagcgcc cggcccttgc acgccgcgat cgccctgccc tgcagattga
     3001 ttcttttcag gcacgcttcc tggagatcgg cggcgaggat cagcagcccc ttcaccgtgt
     3061 ccaccaggtg gtaggtgact tccgggtgga cgttgagcgc ctggttggcg ggaatcgtcc
     3121 acggcgtggt ggtccagacg acgatgaagc cgggcttgtc gatgcgggca ttgaacgctt
     3181 tcgccagctt ctcgttctcc acgaagggaa agccgacgtc gatggcggga tccttgcggt
     3241 cctcgtactc gacctcggcc tcggcgagcg ccgaaccgca gtcgaagcac cagttcaccg
     3301 gcttgaggcc gcgatagacg tagcccttct cgagcagctt gcccagcacc cggatctcgt
     3361 cggcctcgtt gcccggcgcc atcatcaggt aggggtggtc ccagtcaccg agcacgccaa
     3421 ggcgctggaa atccttcttc tggttggcga tctgctcggt ggcgaacgcc cggcagagct
     3481 tgagcgtctg ctcgacgggc aggttcttgc cgtgctgctt ttcgatctgc acttcgatcg
     3541 gcatgccgtg gcagtcccag cccggcacgt agggtgcgtc gaagccggcg agcagcttcg
     3601 atttgaccac catgtccttc aggatcttgt tgaccgcggt gccgatgtgg atgtcgccgt
     3661 tggcgtacgg cggcccgtcg tgcagcacga aggtcgggcg ccccctggcc gccgcgcgca
     3721 ggcgccggta gacgccctgg tcctgccatt gcttcacctg cagcggctcg cgccgggcga
     3781 ggtcgccgcg catcgcgaac ggcgtatcgg ggaggttcag cgtgctcttg tagtcaggca
     3841 ttcgtcttct tcttggcaaa atggctccgc gcctgcgccg cgtcgcgcgc gatcgccgcg
     3901 cgcagcgcgc ccaggtcgtc gtacttctcc tcgtcgcgca gcttggcgac gaacctcacc
     3961 tgcaggtgcc gccggtacag ttccccggcg aaatccagca ggtgcacttc cagcagcggc
     4021 agcgccgtgg ggttgaccgt cggccgccgt cccacgctcg ccacccccgg gcgccaggcc
     4081 gcctcgtcct cgacgcggca ttcgacgacg aagatccctc ccagcgcggg ccgcctgcgc
     4141 agcacgatgt tcgccgtcgg gaaaccgagc tcgcgcccga gtttctcgcc gtgcgccacg
     4201 cggccgctca tggcgtaggg cctgccgagc aggcgctcgg cggcgcgcag gtcgccggcg
     4261 gcgagcgccg cgcgcaccgc cgaactggat acgcgcgcgc cctgcagcgc gacatccggc
     4321 atcgcttcca gctcgaagcc gtggcgcgcg gccagcgcgc cgagcagcgc gtagtcgccg
     4381 gtgcgccgcg ccccgaagcg gaagtcgcgc cccaccagca gccaggcggc gcgcaggccc
     4441 tgcgccacga tctgctcgac gaagcgctcc gcggcgagcg ccgcgagccg cgcgtcgaag
     4501 cgcgccacgt gcacccggcc caccccggcg cgcgccatca gttccagctt ttcgcgcagg
     4561 cgcgtcagcc gcgccggggc cgtcgccggc gcgaagaact cgcgcgggtg cggctcgaag
     4621 gtcagcacgc aggcgggcac ccgcagctcg cgcgcgcgcg ccaccgtgcg ctcgagcatc
     4681 gcctgatggc cgaggtgcac gccgtcgaaa ttgccgatgg taagcgccat ccggctgccc
     4741 cccggcgcaa tggacccgtg cgtgacctgc atcagcgcgt gtgtaaaatg ctgaattata
     4801 gtggctttca gggaggctcc gatgcagacc ctcgaacagc agatgagttt ctacgcggcc
     4861 taccaccagg acgcgcgcaa caaggcctcg cacttcgtcg gcgtgccgat gatcatcttc
     4921 ggcctgttca tcgcgctggg ctgggcgcgc gtggagatcg gcggcgtccc cgtcacggcg
     4981 gcaatgctgc tcgccgccgc ggtgctggtc tggtatttcc tgctcgacgt gccgctcgcg
     5041 ctcgccatgc tggcagtcaa tgcggccctg ctggtcctcg cggacatggt gtcggtgcag
     5101 ccgatcggcg ccgggctcct ctggttcctt gcgttcttcg gcggcggctg ggtgatccag
     5161 ctcgtcggcc acgtctggga gggccgcaag cccgccctgg tggacaactt cttccagatc
     5221 ttcgtcgcgc ccatcttcct cgcggcggag gtgttcttcg ccctgggcta caagcccgcg
     5281 ctgcacgccg cggtgcagaa gcgcgccctg gagatgcgcg ccgcggccgg cgtggccgag
     5341 cgcaccgccg ccgcctagcg cctcagttca ggcgcgcgca cagcgcgccg agctccacca
     5401 gcctggcgcg cacctcgtcg atgtccggcg cctcgggcgc cagcgtggaa tagtcctgca
     5461 ggtccttgag cgccgcgcgg tagcactcca ggcgctggta cagcaggccg cgctcgcgcc
     5521 gctcgccgtg cgccgcgggc gccacctcga gcatgcggtt gagcacctcc agcaggcgct
     5581 gcggctggtc ggtgttgcgg tagatcccct tcaggttgcg cagcagccgc gccaggatct
     5641 ggcgcttgcc cgccggctcg aggaactggt cgagcggcag ctgggccacc ggcacgttgc
     5701 cggtggcgtt ctcgggaatc acgcgcgcca gccgctcgcg caggtcggct tccgactgcg
     5761 gcgcgccgcc ggagaacggg tcgagcacca gcgtcgcccc gcgcagccgc aggctcacca
     5821 ggaaatgccc gggaaaggac acgccctcca gcgtcaggcc gacgcggcgc ccgatctcga
     5881 ggtacagcac cgacagggtg atcgggatgc cggtgcgccg gtcgaggacc tcgttcaggt
     5941 agctgttgcg cgggtcgtag tagtcgtctg cattgccgct gtagcccagg tcgtcgaaca
     6001 ggaactcgtt gagcgccacc acccgcgcct cggcacttcc gtcgcgcggc aggcggccgc
     6061 gcaggcgcgc ggcgaggcgt tcgatctcgc ccaggtagcc ctccacgtcc agccccgggt
     6121 aggcgtcccg cgcgatgagc aggcaggcgc gtcccaggtc gatgccggcc tcctcgcgcg
     6181 ccatcagctc ggcgaacggg tgcagccgct ccatcacccc gcctcgcgcc gcgcgaaatc
     6241 gcgcagccgg aatcccatgg cgagcagcag cagcccgtag ccgagcgcac ctccgggaac
     6301 cagcacggcg atccggagca gcttgtcctg ccagggcgcc tgaagccacc acgcggccgc
     6361 gcccatcgcg aaatgcagtg ccgccgccat cgccgccaca gccagcgtta tcttcagcgt
     6421 gaacaggatc cagcccggct gcggcgcgta gatgccggcc ttgcgcaggc cgcggtagag
     6481 gagcgccgcg ttcaggcagg cgcccagccc gatggccagc gcgagccccg cgtgcttgaa
     6541 cggcacgatg aaggcgaggt tcatcagttg cgtcgcggcg agcgtcgcga ggccgatctt
     6601 caccggcgtg gccacgttct ggcgggcgta gaaccccggc gccaggatct tcaccaggat
     6661 catgccgacc agccccaggc tgtaggccat cagcgcctgg cgcgtcatcc acacgtcctc
     6721 cgcggtgaag cgcccgtaat ggaacagcgt ggcgatgagc ggcaccgcca ggacggcgag
     6781 cgccgccgcc gccggcaggg cgaggagcag cgtcacgcgc aggccccagt cgagcagctg
     6841 gccgtaccgc gcgccgtcgc cgccggcgtg gtggcgcgac aggctgggca gcaggatggt
     6901 gccgacggcg acgcccagca cgccggcggg gaattccatc aggcggtcgg cgtagtacag
     6961 ccaggacacg ctgcccgagg cgaggaagga ggcgaagatg gtgttgatga ggagagaaat
     7021 ctgcgccacc gacaccccga acagcgccgg cagcatcagc ttgagcacgc gctgcacgcc
     7081 gggatggcgc cagtcgaggc gccagcgcgg cagcatgccg atgcgcgcga ggaacggcag
     7141 ctgcagcgcg agctgcagcg cgccgccgac gaacaccgcc caggcgagcg ccagcaccgg
     7201 cgggtcgaag cgctcggcga agaatgccgc cgcgacgatg aacgacacgt tgagcagcac
     7261 cggggtgaac gccggcacgg cgaagcggct ccaggtgttg agcacgccgg cggccagcgc
     7321 caccagggag atgaagaaga tgtacggaaa ggtgatgcgc agcatctgca ccgtgaggcc
     7381 gaacttctcc ggctcggcgt ggaaccagcc cggggcgaac aggtagccga tgagcggcgc
     7441 cgccagcatg ccgagcgcgg tcgccgccag cagcgcgagg aacagcagcg tgccgatcgc
     7501 atccaccagc gagcgcgtct cctcgggcgt ctggcggttg cgggtctccg acaggatcgg
     7561 cacgaaggcc tgcgagaacg cgccctcggc gaacatgcgg cgcagcaggt tcgggatgcg
     7621 gaaggcgacg aagaaggcgt cggcggcgag gccggcgccg aacaggcgcg cgatgaagaa
     7681 gtcgcgcacg tagcccagca cgcgcgacag gaaagtcatg ctgctcacgg tggccagggc
     7741 acgcaggagg ttcatgctag aattcgcccc cttttcgcaa ccggttcaag gccatggcca
     7801 acatcaagtc ctcgcgcaag tccgcccgcc aggcgatcgt gcgccgcgcg cacaatttca
     7861 ccctgcgcac cgaagtgcgc tccgccatca agcgcgtcaa gaaggcgatc gccgcgggcg
     7921 acaaggccgc cgccgccgcg gcgctggaga agtcgcgcag cgtgatcgac cgcgtcgccg
     7981 ccaagggcgt cctgcaccgc aacgccgccg cgcgccacaa gagccgcctc gcccagtcca
     8041 tcaaggcgat gcagtagggc ttcaatcggt atcgccggcc cgctgcagct tggcgagctc
     8101 ggtgcgctcc gggacgcgcc cgtcgcgggt gcgcagttcg ttgtccttgg cgaagctcag
     8161 caggaagcgg taggccatcg gctcgaccgc ctcgagccgc gtatccacca ccacgcagcg
     8221 cacgccgccg gcgttcacgg gatggacgaa cggcgagtac tgcatgcgct ggtcgctgcc
     8281 gaaggcggcc atcacgccgc acaggcgctc ggcccagtcg ctcggccgga agggcttgcc
     8341 gtcgagcgtg acgccctgga tgacgaactc gacgatggcc ggctcgggca tgcggaatta
     8401 tacttgcgcc cctcatggcc aagccgcaca gccgcatcct cgtcgtcaac gacgagcacc
     8461 tcgtcctgcg cgagttcgtc aagggcctga acgcggcggc gcgctcgctc gacaacccgc
     8521 tcggcatcag cttcgccggc gtgaccaccg ccaaggaagc gctcgccgcg atcgagtccg
     8581 atggcgacat ccaggccgtg ctggtggacg accggctgta caccctcggc aagcacaacg
     8641 gctccaggaa gctgcaggtc tccgcgctcg agttcgtgca gcgcatcacg cgctttcgcc
     8701 ccgagctcga cgtctacatc ctcatcgcgc aggagaagga ggacgacgtg gtggacgcgc
     8761 tgttcaccga gtcggtggac ggctatttct accgcgagga gcgcgactac cgcggcatct
     8821 accgcatcct caacgcgcag atccaggagc gcgcgcgcac gccgttcacc gaccagctga
     8881 agaactacgt gtggatggcc aaggaccagt ggcacacgcc ggggcactcc tcgggcgagt
     8941 ccttgcgcgg cagcccctgg gtgaacgact tctacgagtt catgggcgag cacgtgttcg
     9001 acgccgacct gtcggtgtcg gtgccgatgc tcgactcgct gatggaaccc aagggcgtga
     9061 tcgccgaggc gcaggccatg gcggccaagg ccttcggcgc gcgccgcacc ttcttcgcca
     9121 ccaacggcac gtcgaccgcc aacaaggtca tcttccagac gctgctcgcc cctggcgaaa
     9181 agctgctgct ggaccgcaac tgccacaaga gcgtgcacca cggcgtggtg ctgtccggcg
     9241 cgcaccccgt gtacctggac tcggcgctca accgcaagta cggcctgtac ggcccggtgc
     9301 cgaagaagac gctgctcgcc gcgatccgca atcacccgga cgcgcaggcg ctgatcctga
     9361 cctcctgcac ctacgacggg ctgcgctacg acctggcgcc gatcgtcgcg gccgcccatg
     9421 cgaaaggcat caaggtgatc gtggacgagg cctggtacgg cttcgcgcgc ttccatccgg
     9481 aattccgtcc cacggcgctg gaagccggcg ccgactacgc gacgcaatcc acgcacaagg
     9541 tgctgtcggc cttctcgcag gcctcgatga tccacgtcaa cgacccgggc ttccgggagc
     9601 atctgtttcg cgagaacttc aacatgcaca cctcgaccag cccgcagtac gggctgatcg
     9661 ccagcctcga cgtggcgcgg aagcaggcgg tgatggaagg ctacaagctg ctgtcgcgca
     9721 ctctcgcgct gggcaaggaa ctgcgcgcgc agatcaactc gaccggcgtg ttccgggtgc
     9781 tcgagctgga ggacctgctg cccgacgagg tgaagcagga cggcatccgc ctcgacccga
     9841 ccaaggtgac catcgacatc tcgggctgcg gcaccaccgt cgacgagctg cagaaggagc
     9901 tgttcgagcg ctacaacgtg cagaccgaga agagcacctt caacaccctc accctgctgc
     9961 tcaccatcgg caccacgcgc tccaaggtga gccgcctgta cgacgcgctg atgcgcatcg
    10021 cgcgcgagcg ccgggcgccg cggcggctgg tgcgcacgcc cgaggtgccg catttcaccc
    10081 ggctgcgcta cctgccgcgc gacgcctact actgcggcgg cgagctggtg ccggtgttcg
    10141 acgacaagga gggcgtcaac cgcgccctcg cgcgccgcat ctgcgccgac cagatcgtgc
    10201 cctacccgcc cggcatcccg gtgctggtgc ccggccaggc gatcacccgc gaaatcgccg
    10261 agtacctggc aagcctgctg cgctcgcaga agcgcatgga actgcacggc atcgtgatgg
    10321 agggctacca gccctgcgtg cgggtgctca ggagcgccga ggagcgcagc ttgcaccgca
    10381 tcggctagcc cattaacatc ccggggacgg cggcttgggc cgccgttttc attcaggggg
    10441 atgccatgag ccacgtcatg aacacctacg cccggcagcc ggtggcgttc gtgcgcggac
    10501 agggcgtctg gctctacgac gaggcgggca agcagtacct cgacgcgctg gccggcatcg
    10561 ccgtcaacac gctcggccac aaccacccgc gcctggtgaa ggcgctcacc gagcagatcg
    10621 gccgcatcat ccacagctcg aacctgttcc aggtgccgct gcaggagcag gccgccgacc
    10681 gcatcgcgca gatcaccggg ctggacgagg tgttcttctg caattccggg ctggaagcca
    10741 acgaggcggc catcaaggtg gcgcgcaagt acgggcacga ccgcggcgtc gccgagcccg
    10801 cgatcatcgt gatggagaag gccttccacg ggcgctcgct cgccacgctc tcggcctccg
    10861 gcagccgcaa ggtgcaggcc ggcttcgagc cgctggtgca gggcttcgtg cgcgtgccgc
    10921 tgaacgacct cgacgcggtg cgccaggtgg cggcgaacaa ccgcaacgtg gtggcggtgt
    10981 tcatcgagcc gatccagggc gaaggcggca tcaacgtctc gcgcctggag tacctgcgcg
    11041 gcctgaagga gatctgcgac gccaaccagt ggctgttcat gtccgacgag gtgcagtgcg
    11101 gcctcggccg caccggcaaa tggttcgtct accagcacgc cggcttccag cccgacgtgg
    11161 tgccgctcgc caaggggctc gccggcggcg tgccggtggg ctgctgcgtg gtcggcaacc
    11221 gggcgaaggg cgtgttcaag cccggcaacc acggctccac cttcggcggc aatccgctcg
    11281 ccatgtgcgg cgtggtgacg acgctggaca ccgtgcgcga cgagggcctg ctggagaacg
    11341 cggcgcgcgt gggcgaggcg atccggggcg gcctcgcggc ggcgctcgcc ggcgtcgccg
    11401 gcgtggtcga ggtgcgcggc atggggctga tgctcggcgt ggagctcgac cggccctgcg
    11461 gcgagctggt gaagatgggc ctggacgccg ggctggtgtg caacgtcacc gccgacaacg
    11521 tgatccggct cctgccggcg ctggtgatga gcgacgtcga gggccggcag gtggtggagc
    11581 ggctggtgcc gctggtgaag gagtttctcg cgcgcagcgg cgcgcaaccc cgcctcgccg
    11641 cggcgcgctg accggccacc gggcccgcgc atggcgaagc tgcgccattt cctgcaattc
    11701 aacgacttct cgcgcgccga gttcgagcgc ctgttcgcgc gcacgcgctg gatcaaggac
    11761 cagttcaagc gctacaagcg ctactggccg ctggaagacc gcacgctggt gatgatcttc
    11821 gagaagcagt ccacgcgcac tcgcctctct ttcgaggccg gcatgcacca gctgggcggc
    11881 gcggccatct tcctcaacac gcgcgattcc cagctggggc gcggcgagcc ggtggaggac
    11941 gcggcgcagg tgatctcgcg catgtgcgac gtggtgatga tccgcacctt cgagcaggag
    12001 atcatcgagc gcttcgccgc caattcgcgc gtgccggtga tcaacgggct gaccaacgag
    12061 taccacccct gccaggtgct ggccgacatc tatacctacg tcgagcagcg cggcgccatc
    12121 aagggcagga ccgtcgcctg gatcggcgac tcgaacaacg tctgcaacac ctggctgcag
    12181 gcggcggagg tgttcgactt caacgtgcac gtctccacgc cgccgggcta cgaggtcgag
    12241 cccgagcgcg ccaacctgta cggcacctcg cactacgagg agttccgcga cccgatgcag
    12301 gcggccaagg gcgcgcacct cgtcaccacc gacgtgtgga ccagcatggg cttcgaggcc
    12361 gagaacgagg cgcgcaagcg cgacttcgag gactggcagg tggacgccga gatgatgcgc
    12421 gcggcgcgca aggacgccct gttcatgcac tgcctgccgg cgcaccgcgg cgaggaagtc
    12481 gccgccgcgg tgatcgacgg caagcagagc gtggtgtggg aggaggcgga gaaccgcctg
    12541 cacacgcaga aggcgctgct cgaattcctc gtgctgggga aggtggaaaa atagtgttcg
    12601 gcgtcgcgga ctactgggcc ttcgtcgttg ccgtggtcgt gttcctcgcc atccccggcg
    12661 tcggcaacct cgccatcatc acctccacca ccaagggccg gatcgccggc ggcctggcgg
    12721 cgacgctggg ggtgatcgtc gcggaccagt tgctcatgtg gctcgccgtc gccggcgtgg
    12781 cggcggtgct caccgcgcat ccggccgtgt tcggcgtcgt gcagtacgcc ggcgcgctct
    12841 acctggccta cctcggcatc cgcatgatcc tcgccaagcc gggcgacgcg ccggtcctcg
    12901 acatggcgcc gcgccgcttc ttccgccagg cgatgctgat cacgctcctc aaccccaagg
    12961 cgatcgtgtt ctacatggcg ttctttccgc tgttcgtcga ccccgcgcgg caccagggac
    13021 tgctcacctt cggcgtgatg gcgctgacga tcgccgtgct gaccttcctc tactgcttga
    13081 tcgtgatcgc catcacgcac ttcgccgccg accgcatgcg cgcgagcccg cgcaccgccg
    13141 tctggctcaa ccgggtcgcc gggaccatgc tcgtcggctt cggcgtcaag ctcagcgttt
    13201 cttaggcttt tccgcgccga ggagcggcac gccgccgtcc ccgagcagcc ccgcctcgcc
    13261 gtacacgcgc agcttgtcgc gcgtgtccgc gatgtcgagg ttgcgcatgg tcagctggcc
    13321 gatgcggtcc gcgggcgtga agaacacctc gtcgcccttt tccatcgtca agcgctcggg
    13381 cttgtagctg aggttcgggc tccgcgtgtc gaggatcgac cagtcgttgc cccggcgcag
    13441 ctccagcgtc acttcgccgg tgacggcctt cgccacccag cgctgcgcgg tctcgcgcag
    13501 catcatggtc tgcgggtcga accagcgccc ctggtacagc agccgcccga gccgccggcc
    13561 gttgctccgg tactgctcga tggtgtcctc gttgtggatg ccggtcacca ggcgctcgta
    13621 ggcgatgtgc agcaaggcca tgcccggcgc ctcgtagatg ccgcggctct tcgcctcgat
    13681 gatgcggttc tcgatctggt cgcacatccc caggccgtgg cggccgccga tggcgttggc
    13741 ctccgccagc agcgccaccg catccctggt ctgctggccg ttcagcgcca ccgggacgcc
    13801 ctcctcgaag cgcaccgcca ccttctcggg cttcaccgcc acttccgcgc gccaggaggc
    13861 gacgcccatg atcggctgca cgatcgacag ccccttgtcg aggaactcga gatccttcgc
    13921 ctcgtgcgtc gcgccgagga tgttggaatc ggtcgaatag gccttctcgg cgcgcatctt
    13981 gtagtcgaag cccgccttgg ccatgtacgc cgacatctcc ttgcggccgc ccagctcgtc
    14041 gatgaagcgc tggtcgagcc agggcttgta gatcctgagc gccgggttgg cgagcaggcc
    14101 gtagcggtag aagcgctcga tgtcgttgcc cttgaaggtg ctgccgtcgc tccagatgcc
    14161 gaccgcgtcc tcgcgcatcg ccaccacgag cagcgtcccg gcgaccgccc ggccgagcgg
    14221 cgtggtgttg aagtaggtcg caccgccggt ggagatgtgg aacgcgccgc attgcagcgc
    14281 cgcgatgccc tcggcgacga gctgcggccg gcactcgacg aggcgcgcct tctcggcgcc
    14341 gtaggcgagc gcctgccgcg ggatcgcggc gcagtcgggc tcgtccggct ggccgaggtt
    14401 cgccgtgtag gcgtagggga tcgcgccttt cgcgcgcatc cagtgcaccg cagcgctcgt
    14461 gtccaggccg cccgagaacg cgatgccgac cttttccccc ttgggaagcg tgccgaggat
    14521 cgtcgccaag tccgtgctct ccgaaaatta gagatgatac ggatagaatg acgatcgtgc
    14581 gcgccctggc ctttccgctc ctcgccgccc tctccttgag ctgcgccgcg gcgcccttcg
    14641 cggtgcggct gggcaccgag cgggtggcgc tcgatgcgcc gcccggcttc gccgacgcgc
    14701 tcgagctggg ctcgccgcgg ctgcaggaac ttgccgaatc gctcacctcc gcgtccaacc
    14761 gcatcctgct gttcgcgctc accgacgcgg acctgcgccg cttcaccctg ggcgatccgc
    14821 cggacctgcg ccgctacctg ctggtcgcca cgccgcgcgc catggagcgc gatcgcgtcg
    14881 gcctggcgca gtttcgcagc tacgccgccg atgcgacgcg cggcctggga aacccggcgc
    14941 ggcccgccga cctgcgccgg cacctcgacg cgcagccgcc gggcaggccc agcatgatcg
    15001 ccgagctgcg ccgcacgccg caggccatca cgctcctggt cggctcgcgc ctcgccggcc
    15061 ccgacggcaa gaacgtgtac ctcctgtcca ccaccaccct gctgctggtg caggccaaga
    15121 cgctcagcct cgcggcctat accggctacg acgacgacgc ggacctcgac tggctgcgcg
    15181 acacgacgct gcgctgggtc gatgagctcc agcgcctcaa ttcccgctga cccggccgag
    15241 ctgagcggcg tcggtcccta cgcgggacgc gttgccgacg cctatcgcgc gcccgacctg
    15301 ctcgccgccc tggccggcct gcccgcattg ctggcggcgc ctgccgcgcg gctggtggcg
    15361 acggggcgca accgcaacct ccgcgtcgag ctgccggtgc atggccggcg cgtcgcggtg
    15421 atggtcaagg ccttcggcgc gcagtcctgg ctgcgcgacc tgcgcgacct gcgccgcggc
    15481 tccaaggcgc ggcgcacctg gcaggcggcg acgcacctcg ccgagcgcgg cgtcggcacg
    15541 ccgccgccga tcgccttcct cgagcgctgg gagggcgcgc gcctgcgcga gagctactac
    15601 ctggccgagt tccaggacgg cgccaccacc ttcagggaag cgctgctcga gctgttcgag
    15661 cgccggccgc cgctcgccgc gcagttcgtg cagctgctgg agtgcgttgc ggcgggcgtg
    15721 cggcgcatgc acgacgcggg cttcctgcac aacgatctcg gcaaccagaa cgtgctgctg
    15781 cggcccggcg gcgacgccgc ctggcgcgac ttcatgctgg tggacctgaa ccgcggccgc
    15841 gtccgccctg cgctcggcct gcgccagcgc gggcgcgacc tgtcgcgcct ggagctgccc
    15901 tcggacctgc tgcaaatact ggtggaaatg tactggcgcg gcacgccgcc gcgcgcgctc
    15961 ctggggacgg cgcgcctgta ccggcggctc tacggcctgc aggtgctcaa gcgccgcctg
    16021 ctgcaggtcc tgcgcggcgg ggcccgcgaa gcgcccgggc gcgactatcc cgcgccgcgc
    16081 gagctgtgga tctgggacga gcccacggca cagccgatca gcgcgctatt gcggcgcgac
    16141 cgcgcgcgcc tgttcccgct gtcgcgctac cgccgcgtgc tggtggacgg cctgcgcgcc
    16201 gcgcccggcg tgtggcgcga ataccgcgcg ctgcggcgcg cggcgtacgc gcatcccgtg
    16261 caattcgccg gccgcatcgg catggcgctg gagccgacgc cggcgacgcg cgagcaggag
    16321 ctggcgctgc tcgccggcct ggggcgcatc cccgcgctgg tgcgcttcta ccaccacgac
    16381 gatgccgcga ggcgcgcgtt ccgcgtggag ctggtgcatg cgctgcatcg cgccgggcac
    16441 ccggtggcgg cggcgctggt gcaggatcgg cgcgcgctgc gcgaaccggc ttcctggcgc
    16501 gcgttcgcac gcgaagtgct ggagcgcatc gccccggtgg tgcaggaggt cgaggtcggc
    16561 cacgcgatca accgcgtgaa gtggggcgtg tggggattcg aggagttgcg cgcgctctac
    16621 gcgccgctgc cggacctgca gtcgcgcttc cccgcgctgc gcttcatggg ccccgcggcg
    16681 atcgacttcg agtacccggc ggtattcccc gccctgcgcg agtggccgcg ggcggtcccg
    16741 ctcgccgcgc tgtcgcacca cctttacgtc gaccgccgcg gggcgcccga gaacccgcag
    16801 gacgggttcg gagcgccgga gaaattcgcg ctgggccgcg ccgccgcccg cgccagcggc
    16861 gcggcacgtt tcatcgtctc ggaggtcaac tggcccctgc acggcatggg cgcgcagtcg
    16921 ccgatccacg cgccctactt cccgccgtgg cgccaggcga gcgacccggg ggtcggcgaa
    16981 gagcagtacg gcgactacct gctgcgctac ctgtgcctgg cgctcggctc gggcatggtg
    17041 gagcgcgtgt actggtggcg cctggcggcg cgcggctacg gcctggcgga cgacgcgcag
    17101 ccggcggcgc tgcggccccg gcccgcctac ttcatgctgc ggcatttcct cgcgctgctg
    17161 ggcgacgcca cgctgctgtc ggcgcgcctg ccggcgcgcc gcggcgagcg gcacgggcgc
    17221 taccgcttcg ccttccgccg tgcagacggc gagatcgtcg ccctgaccta tgcgcacggg
    17281 cccgggctgc cgtttcccgc ggacgatgcc tgcgcccggg tggaggacgc cttcggcgag
    17341 acggcagcgc ccgccggcct cggcggccgg ccggtctacc tgcgcggcgc ccgcgcgtag
    17401 aatcgcgcca tgccctcctt cgacatcgtc tcgcaggtcg accagcagga agtgaggaac
    17461 gccgtcgagc aggcgaacaa ggaaatccag aaccgctacg acttcaaggg ttcggacgcg
    17521 cgcatcgagc aggacggcct ggcgctcacc gtgttcgccg acgacgagtt caagctcggc
    17581 caggtcatgg acgtgctgcg ccagcgcatg gccaagcgcg gcatcgacgt gcgcagcctg
    17641 gagctgggcg cggtggagaa gatctccggc gacaaggcga agcgaccggt caccgtgaag
    17701 accggcatcg cgcaggagaa ggccaagcag atccagaagc tggtgaagga cgccaggctc
    17761 aaggtgcagc cctcgatcca gggcgacctg gtgcgcatct cgggcgccaa gaaggacgag
    17821 ctgcaggcgg cgatccagct ggtgcggcag caggtcgccg acctgcccct gcaattcatc
    17881 aacttccgcg actgagccct tcgcaccagc gctcgtagat ccggtcggcg accgcgtgca
    17941 gcgccgtcag gcgccgctcc atgtccttgc gcacctgccc gggctgctgc acgccggcgc
    18001 tggcgcgcga ggcctcgatc tcgcgcgcgc cgccctgcag ccagtcgccg atcagttccg
    18061 cggtcatctc gacgtggcat tgcatgccga agtgcttgcc gagggcgaag gcctggttgt
    18121 cgcagtgtgc gttctccagc acgcgcgtgc cgcccggcgg gatggagaag gtctcgccgt
    18181 gccagtggaa cgcctcgaag gcgcgcagcc cgccgagcca ctcgcgcgcc acgccgttgg
    18241 cgcaggcccg cacctcgccc cagccgatct ccgtcaccgg cgcggcgcgc acctcgccgc
    18301 cgagcgcccg ggccatcagc tgcccgccga ggcagtgccc gagcaccggc acgtccttgc
    18361 gcaccgcgtc gccgatcagc gcgagcgccg gaccgatcca gggcaggtcg tcgttcgcgc
    18421 tcatcggccc gcccatgaag gcgaggccgg cgtagcgccg ggcgtcgcgc ggcacgccgc
    18481 ggccctcgtc gagcgcgacg agcgtccagg gaatcgactt gcgctcgagg gcggtggcaa
    18541 aatagccggg accctcgctc ggggcatggc ggaatatggc gaccggcttc atgacgcggc
    18601 attgtagtgc gctcgtcctc gcgctggcgg ccctgccggc cctggccctc gacgtgagcc
    18661 tgatcggcac cttcggcgac aaggcggcgg tgctggtgat cgacggcggc gcgccgaaga
    18721 cggtcaaggt cgggcagcgg gccggcggcg tcacgctggt ctcggtggag cgcgaccgcg
    18781 cggtgatcga agccgacggc ggccgccgca cgctggcgcg cggccagcat tacatcagcg
    18841 gcgccgccgg cgaccagcgc cagagcgtga cgctggccgc cgactcgcgc ggccacttct
    18901 tcagcgacgg cgcggtcaac ggcggcgcgg tgcgcttcgt cgtggacacc ggcgccaccc
    18961 tgatcgcgct gccggcgagc gacgcggtgc gcctcggcct ggattaccgc agcgggaaac
    19021 tggcgctcac caagaccgcc ggcggcctgg tgcccgtgta ccgcgtcaag ctggacagcg
    19081 tgcgcctggg cggcatcgag ctcgcggcgg tcgagggcat cgtcatcgag cagggactgg
    19141 atatcgccct gctcggcatg agcttcctca accgcgtcga gatgcgccac gagggccgcg
    19201 tgatgactct cacgcgccga ttctgactcc ccgttacagc gcgaccgtca cccgcagaaa
    19261 cgtcgccgga atggaggtct tgtccttgcg ggtgctcttg ttctgcgcct gcaccaggtc
    19321 ttcgagctgc cgctgcaggt ccgtcgcgcg cccattcttc tccgcggcgt cgaaggcgtt
    19381 catcgtcggc ccgtagtatt tcctgaacag gccgacgaag tcctccggcg tgccggacaa
    19441 gttgaaggtg aaagtatcgc gcacgaagga aatttttttc gccgggacgc cggcagccgc
    19501 gaagcgttcc gtgacgttgg cctcgatgcc ccaggtcatc ggactgacga aaccatcggg
    19561 cggcggcggc gtgaacgccg aactgatttt caggatctgc gcgaccagcg tcggatcgtt
    19621 gggaatccag ttgcccatga tgatgcgtcc cccgggccgg gtgacgcgga ccatttcttt
    19681 cgccacgtcg aagggcttgg gcgcaaacat cgcgccgaag atggtgacgg tcaggtcaaa
    19741 gctgcgatcc ggcacgccct tcatgtccga agcgtcgccc tcctggaacc tgcagttggc
    19801 aaggccgagg tccttcgcgc gcttgtttcc cgcggcgacc aggttggccg cgatatccac
    19861 gcccagaaca tcggcgccgc gtttcgctgc cgggatggcg gtggttccgt cgccgcagcc
    19921 gaggtcgagc accttgaggc ccttcgtgat gccgaacgtc tcaactagcg cttcgccgct
    19981 ttcgcgcatg gtgctcgcga cgcgcgtgaa gtcgcctttt tcccacagtg ccttgtttgg
    20041 gttcatccga tgctccctct cgccttcaat agtcgataaa actgcgggct atcacgcccc
    20101 cccaccggaa ccacttgcca atcagcctaa tacgaatcta cccgccccga taggcgccag
    20161 gaaacgcaat ttcatccgat tccgagacag cacctatttc cccggcacca gcggcacgaa
    20221 cctgacgggc agcacgctgc ggcgctccac gctgccgtcg gcgcgcttct ccagcagcag
    20281 aagctcctgc gccgccaggc tggcgcccac cgggatcacc atgcggccgc ccggtctgag
    20341 ctggtccacc agcgcctgcg gtacctgggg cgcggcggcc gtcaccacga tgccgtcgaa
    20401 cggcgcctgc tccggccacc cggcgtagcc gtcgccgatc ctgacttcga cgttgctgta
    20461 gccgagggtc ctcagccgtt gcgccgcttc cttgcccaat gccgggatga gctcgatcga
    20521 gtaaacagtc gagacaatct cggccagcat cgccgcctgg tagcccgaac cggtgccgat
    20581 ctcgagcacg cgctgcccgg gccggggcgc gatcagctcg gtggacagcg cgacgatgta
    20641 gggctgcgag atggtctggc cggcgccgat gggaaccggg tggttgccgt acgccaggtc
    20701 ctgccggtcc gcgggaacgt agcgatggcg ctcgagcttg ccgagcgcgg cgcgcaccgg
    20761 cgcggaaagc gccgcgatgc cggtctcgcg ccgggtggcg gccaccatcg cctcgacctc
    20821 ggcgaccagg cgcgcgcgct gcgcggcatg gtcctgggcg tcggcggcgc tcatgccgag
    20881 ccaggcgaga acgcagctag ccagccttct tgtcgcgctc gatgagggcg taggcggagt
    20941 ggttgtggat cgactcgaaa ttctccgact cgaccacgta gcgcgtgatg cgcttgtccc
    21001 ggttcagcgc cgcggccacg tcgcgcacca tgtcctcgac gaacttgggg ttgtcgtagg
    21061 cgcgctcggt gacgtacttc tcgtcggggc gcttgagcag gccgtagagc tcgctcgagg
    21121 cctgcttttc caccaggtcg acgatctcct cgatccacac gaagtccccg gtatgcgcgg
    21181 tgaccgtgac gtgcgagcgc tggttgtgcg cgccgtagtc cgagatcttc ttcgagcagg
    21241 gacacaggct ggtcaccggc accagcacct tcatggcgaa gcactgctcg cccttcagga
    21301 tctgcgcgac gaaggtcacc tcgtagtcca tcaggctgcg cacgccggac accggcgccg
    21361 ccttgtcgat gaagtagggg aaggccatct cgatgcgccc ctccttggcc tcgagcttgt
    21421 ccaccatctc gcgcagcatc aggttgaacg atgcgaccgt gagctcgcgc tcgtgcgcgt
    21481 tcaggatctc gacgaagcgc gacatgtggg tgcccttgaa ctggtgcggc aggcccacgt
    21541 acatgttgaa gttggcgatg gtgtgctgca ccccgcccgc gcgctgcatc acctggacgg
    21601 ggtggcggat cgccttgatg ccgaccttgt cgatggcgag cttgcgcgca tcgggcaggc
    21661 tctggatgtc cgggatgtgg tgcgggtcct tggcgttcat ggcgccatta taactcccga
    21721 gtaatttact cggctattac cgtttcgatc gcctgccgga tccctggcgc atccaacccc
    21781 acggaagata agagttttcc cggttctccg tggtcgacga agcggtccgg caggccgagc
    21841 agcagcacgc ggcgctcgat gcccaactgg ttcagcgcct cgcacacggc gctgcccgcc
    21901 ccgcccatca cctggtgctc ttcgaccgtc accagcaggg cgtggtcctg cgccagctgc
    21961 cgcaccagct cgacgtccag aggcttcacg aagcgcatgt tggccacagt ggcgtccagc
    22021 gcctcggcag cctccagcgc gggcttcagc atcgcgccga aggcgaggat ggcgacgcgc
    22081 ctgccccggc gccgcagctc gcccttgccc accggcaggt cgctcaagtc cttgccggcc
    22141 tctcccgggg cgacgccgcg cgggtagcgc acggccgccg gcttgccgag gcggaacgcc
    22201 gtggtcagca tctgccggca ttcgttcgcg tccgagggcg ccatcaccgt gaggttgggc
    22261 accgcgcgca ggtaggcgta gtcgaaagcg ccgttgtgcg tagcgccgtc gccgcccacc
    22321 agcccgccgc ggtccagggc gaagctcacc ggcaggttct ggatggcgac gtcgtggatc
    22381 agctggtcgt agccgcgctg caggaaagtg gagtagatcg ccaccaccgg gcgcaggccc
    22441 tcgcaggcga ggcccgcggc gaaggtcacc gcgtgctgct cggcgatgcc gacgtcgaaa
    22501 tagcgcgcgg ggaattcctc cgagtagcgc accatgcccg agccctcgcg catcgccggc
    22561 gtcaccgcga ccaggcgctc gtcggcccgc gccatgtcgc acagccagtc gccgaacacc
    22621 tggctgtagg tcggcttgcc gggcgccgcc ttctgcagcc cctgcgccgg gtcgaacttg
    22681 cccgggccgt ggtaggcgac cgggtcgtcc tcggcgagct tgtagccctg gcccttgcgc
    22741 gtgacgatgt gcaggaactg cggacccttg agctgcttga tgttggccag ggtcgcgatc
    22801 agcgtgtcca ggtcgtggcc gtcgatcggg ccgatgtagt tgaagccgaa ctcctcgaac
    22861 agcgtggaag gcgtcaccat gcccttcagg tgctcctcgg tgcggcgcgc gaggtcccag
    22921 accccgacgc gcttgagcac gtgctcgctc gcgcgccgcg cggtcgaata catgcgcccc
    22981 gacagcagcc gcgccaggta gttggtgagc gccccgaccg gcggcgagat cgacatctcg
    23041 ttgtcgttca gcaccaccag caggtccgcg tccatggcgc cggcgttgtt catcgcctcg
    23101 aaggccatgc cggccgacat cgcgccgtcg ccgatcacgg cgatgcactt gcgcgcctcg
    23161 cccttcgcct tcgccgccac cgccatgccg agcgccgccg agatcgaggt cgaggaatgc
    23221 gcggtgccga aggtgtcgta gggcgactcg gcgcggcgcg ggaagccgga gatgccgtcc
    23281 tgcatgcgca gccgcgccat cgccgcgcgc cgcccggtga ggatcttgtg cgcgtaggtc
    23341 tggtggccga cgtcccagac gatgcggtcc tccggcgtgt cgaacaccgc gtgcagcgcg
    23401 atggtcagct cgaccgtgcc caggttcgac gacaggtgcc ccccggtctt ggacaccgag
    23461 tcgagcacga aggcgcgcag ctcctcggcc agctggtgca attgcctgcg ctccagcttg
    23521 cgcagcgcgg cgggatcgtc gatcgtctcg agcagctcga acatcgctag aacttccgca
    23581 gcacgatgaa atcggcgagc ccgcgcaggc gctgcgcgcc cgcgcccagc ggctcgagcg
    23641 cgcgcagcgc attctggcgc agctcctcgg ccagttcgcg cgcgcgcggc aggcccatca
    23701 ccgacacgta ggtcgacttg ccctggcgcg cgtccttgcc cgatgtcttg cccagcgtcg
    23761 cggtgctcgc ctcgtggtcg agcaggtcat ccaccacctg gaacgcgagg cccacatact
    23821 tcgcatactg gtccaggcgc gcgcgcaggt cggcggcggg cgcgccggcg cagcaaacgc
    23881 cgagcagcac cgcagcgcgg atcagcgcgc cagtcttgtg gatgtgcatg gattcgagcg
    23941 ccggcggctc gagctgcttg cccgtcgact ccaggtcgat ctgctggccg cccgccatgc
    24001 cgcgcgagcc ggcggcggcg gcgagcagcc tgaccatctc gagctgcgcg cgcgcatcgt
    24061 cggcgagcgg gtgctcggcg agcagctgga aggcgagcga ctgcagcgcg tcgcccgcca
    24121 gcagcgcggt ggcctcgtcg aattcgacgt gcgccgtcgg cttgccgcgg cgaagcacgt
    24181 cgtcgtccat gcacggcagg tcgtcgtgca ccagggagta ggcgtggatc agctcgaccg
    24241 ccgcggcggc cacctccagc cgctcggggc gggcctgcgc cacctcgccg gcggcgaaca
    24301 ccagcagcgg gcgcacgcgc ttgccgcctt ccagcgtggc gtagcgcatc gcctcgtgca
    24361 ggcgcgccgg cgcgatctgc gcgccgggca gcaggcgcgc cagcgccgcc tccatccgcg
    24421 cctggacgcc gcccatccag gccgcgaaag cggcgtcagg cgcgctcacc gggctccgca
    24481 tcggcggcga ggcgcttcaa cacctcgcct tcgagcacct ggacctgccg ctgcgcggcc
    24541 tgcaggcgct cctggcagta gcgcgccagt tccaggccgc gcttgtgcgc ttccagcgcc
    24601 tgctccaggg acagcttgcc gccttccatc tgcgccacca gcttctccag ctcggccagc
    24661 gccttctcga agcccggctc ctgcgcggtt ttcgccatgg catgaactta gccgaggcgc
    24721 ccgcgggcgg tcaaaccttg cctggggtag aattcgcccc attttttcgc cacccccgga
    24781 ctccatgtcg gatctgccgc ccctgcgcca gctgaagccc gcgacctgcc agttgccggt
    24841 cagctggtat ttcgacccgc aaatcttcgc ggcggagaag aagctcctgt tcgacgccgg
    24901 cgccaactac gtcggccacg agctctgggt gccgcagccg ggcgactacc gcacgctcga
    24961 gtggctggac cacgccaagg tgctggtgcg gaacgacacc ggcgtcgagc tgctgtccaa
    25021 cgtctgccgc caccgccagt cgatcatgct cgagggccgc ggccgcacgg aaaacatcgt
    25081 ctgcccgctg caccgctgga cctacgacct gaggggcgag ctgctcggcg cgccgcgctt
    25141 cgccgagcag ccctgcgtca ggctgcattc gacgccgctc agcaactggc agggcctgct
    25201 gttcgccggg ccggccgacc cgcgccaggc gctcgccggc atgcgcaacc tcggcgactg
    25261 ggacttctcc ggctaccagc tcgaccgcgt gatggtggag gagtacaact tcaactggaa
    25321 gaccttcatc gagacctacc tcgaggtcta ccacgtcgac ttcttccatc ccggcctggg
    25381 caacttcacc gactgcgaca atttcgccgt cgactacggc cgcgactggt cggtgcagat
    25441 cgtgcccgcc aaggccggcc tgcaaaaacc cggcacgccc gcgtaccgca agtggcagga
    25501 cgcctgcctg cagcagctct cgggccgcac gccgaagcag ggcgcgctgt ggatgaccta
    25561 cttcccgggg ctgatgctgg agtggtatcc gaacgtgctg gtggtgagcc gcctcgtccc
    25621 gcgctcgccg acgcgcacca ccaacgtggt cgagttctac tatcccgagg aaatcgtgct
    25681 cttcgagcgc gagttcatcg aggcgcagca ggccgcctac gtggagaccg cgatcgagga
    25741 cgacgaaatc tgcctgcgca tggaccgcgg ccggcgcgcg ctgtgggaag agggccgcga
    25801 cgacgccggt ccctaccagt cgccgatgga agacaccatg gtgcacttcc acgaatgggt
    25861 gcggcggggc ctcgggcgct gaacacgctc gtcgccaccg gcgagctggc gcgccacccg
    25921 gaatggcggg tgttcgactg ccggcacgat ctcttcaagc acgacctggg cgagcagcag
    25981 taccgcgcgt cgcacatccc cggcgccgcc ttcgcgcacc tcgaccgcga cctctcggcg
    26041 ccgaagaacg gcgccaacgg ccgccatccc ctgcccgatc cgaacgtgtt catcgcctgg
    26101 ctcggcaggc agggattgaa gcccgcggac caggtcgtcg cctacgacgg cggcccgggc
    26161 gctatggccg cgcgcctgtg gtggatgctg cgctgggtcg ggcacgacaa ggtggccgtg
    26221 ctcgacggcg gcttcgccaa gtgggtgcag gaaggccggc cggtgacggc ggacatcccc
    26281 gcgttcccgc ccaccgcata tccgggaaag gtgcgcgcgg accgcgccgt cgatgtgacc
    26341 tttgtccagc agcaccttgg ggagatatcg ctgctggatg gacgcgcgcc ggcgcgctgg
    26401 cgcggcgaga ccgagccgat cgacccggtg gccgggcgca tccccggctc gctcaaccgc
    26461 ttcagcagcg acaacctgac ggcggagggc acgttcaagc agcccgaggt gctgaagcac
    26521 gagttcctct cggtgctcgg cacgcgcaag ctcgaggcga tcgtcaacag ctgcggctcg
    26581 ggcgtcgccg cctgccacaa cctgctggcg atggagatcg ccggcctgaa gggcgcgcgc
    26641 ctgtacgccg gttcctggag cgagtggatc gcggatcccg cgagaccgcg tgagcgcggc
    26701 tgacgccctc ctcgtcgtcc acttcgcgat cgtcgctttc atcgccggcg ggctggtcct
    26761 gacctgggtc ggggccgtcc tgggctgggg ctgggtgcgc aatcgctggt ttcgctatgt
    26821 gcacctggcg gcgatcgcct tcgtcgcggt ggaggcgctc atcggcatga cctgtccgct
    26881 gacggaatgg gaagatgcgc tgcgcggcgg cgcgcgcccc gagtctttca tcgggcgctg
    26941 ggtgcagcaa ctgctctact accgcgcgcc ggaatgggtg ttcaccacgc tctaccttgc
    27001 ctgggctgcg gccacgctcc tcaccttgcg cctggtcccg cccgcaaggt aggctaggtc
    27061 tccacccgag gaggaggaga cgacaatgaa caaaccattg cggatgcgca ctgccgcgcc
    27121 cgtgctcgcc gcggccgccc tgctcgccgc ccccgcgctg gcgcagacgg tcgacggtcc
    27181 gaaagtggac tggaagctcg ccacctgggg caagccgcgc gccttcaccg ccggcatcga
    27241 aacgctcgcc aagcacgtcg gcgagcgcac cggcggcaag ttcaccatca ccatcggcta
    27301 cagcaccttc gggggcgaga aggagatcct cgacatcctg aactccggcg gcctgcagat
    27361 gaccaccatc tgctcgtcct accacccgga caagcacccg gcctataccg gcctcgacct
    27421 gccgttcctg ccgctgccgg acttcgacac gcagtgggcg gtgcacgacg cctaccacgc
    27481 gcacccgtac atccagaagg agctggcgcg ctggaacgcg atgccctact cctccaacct
    27541 gctgccgcag tacgagttca tcggcaaggg caaggcgccc aggtcgctcg ccgacttcaa
    27601 gggcatgcgg gtgcgcgcca tcggcggcat cggcgacgcg atgaagaacc tcggcgcggt
    27661 gcccacctcg gtgcccgcca ccgaggtcta cacctcgatc gagcgcggca ccgtggaggc
    27721 ggcctcgttt ccctccacct atgcgcatgc tgcctacaag atccacgagg tgggcagctg
    27781 gtacaccacc aacctgtcgc ccggcaccca ggcctgcccg acgctcatca accgcaacgc
    27841 ctgggcgcag atcccggcgc agtaccagaa gctgttcgag gaggcgcggc ccagggtgaa
    27901 gcaggcgctg accaaggcct acggcgccgc ggacgagaag aacctgccgc tgttcaagcg
    27961 ccggctcgct ttcgtcacct acacgccggc cgagctcgcg cagttccacc aggtgggcgc
    28021 caagcccgta tgggatgcct gggtggccga gatgagcgcc aagggcgttc cggcgcgcga
    28081 attgctcgac ctgatcttcg cctccgccaa ggcgcgcaag tagcggcgcg ccgagacggg
    28141 ccgagcgcgg tggccggacc gtccgcatcc gcagaccacg cgccccggcc gccggccggg
    28201 gcgccgctgc tcgcgcgcgc cgactggcgc ctggcgcggc tggagaacgg cctcaacctc
    28261 gtcgccgccg cggccatctt cggcgtgatg gccttcagcg tggcacagat cgtgagccgc
    28321 agcctgtcgt cctggctgca cgcgctgatc cccgcgatcc cgcagatcgc gatccacggt
    28381 tacatcgact acatcgagtt catcgccgtg ttgtatgccg tcctcggcat cgcctactgc
    28441 cagcgcctgg gcggccacat ccgcatggag atcgtggtcg ccaatttccg cggacgcgcg
    28501 ctgtggatga tggaagcgct ggcggtgctg ctcgcggtgg cggtcaccgt cctgctgatc
    28561 gccggcaccg gggacaactt cctcaacgcc tggaccaagg gcgactcgtc gatggacatc
    28621 aagctgcagc tgtggccctc gaagctcgtg gtgccgctga tgctcggcgt gctgctcgcg
    28681 cggctgctgc tgcagctctg ggcctacctg cggctcgccg ccgatccgtc gctcgcgccg
    28741 atcgcggtgc cggtgcccga gacccccgag caggcggcgc ggcgcgagat cgaggaagcg
    28801 atcggcaagc tcggcccgga cgacgccccg cgcggctgat gccatggacc cgctgaccat
    28861 cggcatctgc ggcgccgccg ccctcgtcgt gctggcggtg ctcggcgtgc gcatcgccta
    28921 tgccgcggcg ctggtcggca cgctcggcct gatgatcctg atgcgggatc cctacgcggc
    28981 cctggtcacc gccggcaccg tgccctacgc caagggcacc agctacgaac tctccgtgct
    29041 gccgatgttc atcctcatcg gctacctcgc ctattacgcc ggcatgacca ccaccagctt
    29101 cgaggcggcc aagcgctggt tcggctggct gcccggcggc ctcgccatcg gcaccatctt
    29161 cgcggtggcc gccttctcgg cggtgtccgg cgcgagcacc gccgcggccg cggtgttcgc
    29221 gcgcgtggcg atcccggaaa tgctcaaggc gggctacaac cgggcgctcg ccgccggcgt
    29281 ggtggcggcg ggctcgacgc tcgattcgct gatcccgccc tcggcgctgc tcgtcatcta
    29341 cggcatcatc gtcgagcagt cgatcggcga cctgctgatc gcgggcttcg tccccggcat
    29401 tctctcggcg ctggtgtact gcgcgatcgt ggtgttgcgc tgcaggctcg acccggcgct
    29461 cggccggccg atccggggct acacctggac gcagcgctgg cagtcgctgc cgccgacggc
    29521 gccgatctgg atcgtggtgg tgctgatctt caccgcgatg ctgttcggct gggcgacgcc
    29581 caccgaggcg ggcgcgctcg gcgcgttctc cacgctgctg ttcgcctggc gccacggcat
    29641 gagctggggc cagttccgcg gcgcgatgct cgacgccgcc aagctctcgg tgatgatctt
    29701 caccatcatc tggggcgtgc tgatcttcgt gaggttcctg ggctacgcca acctgccgct
    29761 cgccttcgcc ggctggatcc agagcctggc gctgcacccc ctgctggtgc tgctcgccat
    29821 ctacttcatc tacctggtgc tcggctgctt catggacgcc atcggcatga tgctgctcac
    29881 cctgccgatc gtgttcccgg tgatcgaggg cctaggctac gacccgatct ggttcggcat
    29941 catcgtggtg aagctctgcg gcatcgggct gctgacgccc ccggtgggcc tgtgcgcctt
    30001 cgtggtgaac ggggtgcgcc ccgacatccc gctcggcgac gtgttccgcg gcatctggcc
    30061 tttcgtgctg gccgacgtcg ccaccgtggc tctgctcacc gcctatccgg agctggtcac
    30121 cttcgtcctg acgctcaagc cgcgctagcg ctggccgagc aggccgtgca ggatgcggcc
    30181 gaaatcggtg ttgtcgtggt agccgcggaa gcgctcggcg ccgggtccga tggcgcccac
    30241 caccaccggc tcggtgctgt gccccgaggt gccccagtag aagccggtcc ggcgcgccac
    30301 catgcggccc agcgcattgc gcaccggata gctgttgttg cgctcgagga tctgcccctt
    30361 gaggatcgcc tcgcgcaggt cggcgtcgag cctgaacccc gggaaatgcg cggcgagcag
    30421 cttgtcgatg ccctcgttcg tcggcttctt ccccagctgc tcgagcgcct tgtcgaagga
    30481 gatgctgatg ccggcgagaa gctgcagctg ggcgtcgccg gtatagaagc ggttcttgct
    30541 cgaggtgctg ctcaggtccc tgaaggcgta ggtagcgctc aggccgccgg tctcgtggtc
    30601 gccggtgacg atgatcaggg tgtcgggcga gccgcgctgg aattcgagcg ccacgcgcag
    30661 ggcttcgtcg aaggcccaca ggtcgcgcat cagcgccgcg gcgtcgttgc ggtggcccgc
    30721 ggtgtcggtg ttctcgttct cgatgaacag gaagaagccc cgcggattgt ccgtcgcgag
    30781 cgccttgagc gcggcgcgca tcatgtcggc gaagctcggc tcgccggcat cgcggtcgat
    30841 cgcgtaggcc atcgactcct cggcgaacag gccgagcagc cgcgggcccg cagccgcctt
    30901 cagctcggcg gcgttgcgcg ccagcgcata gcccttgccg cggaacccct cgaggtgcgc
    30961 cggcttgaac tggtcggcgc cgccgcccat caggacgtcg ggcgcgagcg ccaggtactg
    31021 gtcgacgatc gaggcggcgt cgcggcggct cctggcgtgc accgagaacg cggccggcga
    31081 agcgtcgtag atctcggcgg tggtgaccag gccgatgcgc ttgccctgcg ccctggccac
    31141 ttcggcggcg gtgcgcgccg gcttgccgtc gggcccgacg gcgatggcgc cgatggtggt
    31201 cttcacgccc gtcgacatcg ccgaggcggc ggaggcggag tccgtcgcga acgcctcgta
    31261 gggatgggtg gtgaggaagc ccaggctgcc ctggtggaac accacgtcgg tgaccgcgaa
    31321 gccggatttg cgcagcgccc ggctggaata gcgccccagc tcccactgcg tggcggcggt
    31381 accgtcggcg aacagcagga tgatgttctt cggctgctgc tgctgcgcga ccggcaccgg
    31441 ggcgcaggca gccaggaaca cggcgaggac cgccagccag cgggtatgtt tgagcatcgc
    31501 acttcccccc tttgcgtcga ttttaccgcc gatgaaccgc gcgcagccgc tgcagcgcct
    31561 cgggcgcgcc gcgcacgcgc agcagcacgc cgtcgccgtc cgcgcgctcc tccagcacct
    31621 cgcaggccgc gaagatctcg ccgcgcagct tctgcgccgc ccagggcagg aacagctccg
    31681 cctcgaccag gtcgcgccgg aagaacgcga cgatcgcctc gcgcagcgcc gcgacgtcgg
    31741 ctgcgcggcg cgcgctcatg acgacgcacc cgggatacct gtcgcgcagc gccgccgcgt
    31801 cgccgccgcg gtcgatcttg ttgaacacca gcagccgcgg cacgcccttg gcgccgatct
    31861 cgtcgagcac ctcctcggtc accgcgagct ggcgctcgaa gcccgcatcg ctcgcgtcga
    31921 cgacgtgggc gagcagtgac gcttccagtg cctcgtcgag cgtcgacttg aacgacgcga
    31981 ccagcccgtg cggcaggttc ttgatgaagc cgactgtgtc gctgaccagg atgcgcggca
    32041 cgctctcggg ctgcagggcg cgcacggtgg tgtcgagcgt ggcgaacagc ttgtcggcga
    32101 ccagcgctgc gttgccggtg agcgcgcgca tcagggtgga cttgcccgcg ttggtgtagc
    32161 cgacgagcgc cacgcgcgcg agatcctggc gctcgttgcg ccgcgcgcgc tgcgtggtgc
    32221 gctccttcgc catcgcgtcg agctccgtct gcagctcggc
//
LOCUS       DASRIE010000196         6807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_203770, whole genome shotgun sequence.
ACCESSION   DASRIE010000196 DASRIE010000000
VERSION     DASRIE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203770"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="VFB53_08250"
     CDS             <1..1886
                     /locus_tag="VFB53_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86760.1"
                     /translation="DWLDYTLSRPDGARIASSLPPSVSAQTALADRESFELAVRNGEP
                     VVGSLAQWPNGQKWGIPLRMPVVENGVARYVLTAVLRPDAFTDLLRAQRLPEGWIAGI
                     ADRHGRFIGRLPPAPHGQLASESWRERMQRSPQGWFRGRTIEGRETYTPYVTSSLSGW
                     ALGIAIPVQTVEAAAWRSLWLVLIMVLLASGIAVGLAVLLGWRIARPIAALAQAAEAA
                     GRGERVGFPLRTPIAELDSLSAALAQAAQAMHDKHELAEREKAALQAADRAKDEFLAM
                     LSHELRNPLAALTAAAHVLRLAAPGDAAAVQARAVVDRQTKHMARLVGDLLDISRVAM
                     GKVALERERFDLAESAKNVINMWRASGRLERHQLALDIRPAWIDADRARIEQILSNLL
                     DNAVKFTPAGRSIAVSVGPQGESARLQVSDQGEGLAPGSAERMFDLFVQGERGLDRAA
                     GGLGVGLALVKRLSQLHGGSVSATSAGPGKGATFTVLLPLVQPATTRSGEVVREPLLA
                     PRRVLIVEDNDDARQMLHEALVYGGHEVREARDGASGLALAAESPPDVALIDIGLPDV
                     DGYEVARRLRASPGGRRMGLIAITGYGQAEDQRLAYEAGFDAHLTKPVAPERLKQVMA
                     GLQ"
     gene            1897..2256
                     /locus_tag="VFB53_08255"
     CDS             1897..2256
                     /locus_tag="VFB53_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86761.1"
                     /translation="MKRLLVVDDDADMRLTLKLALELAGYAVEVAANGREALHAQRRQ
                     PADVLITDIFMPDADGFEAIDAFRKEFPRTKIVVVSGGAQFSKRDYLPDAELIGVDAT
                     LQKPFDVDTLLGLIKTL"
     gene            2260..2457
                     /locus_tag="VFB53_08260"
     CDS             2260..2457
                     /locus_tag="VFB53_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86762.1"
                     /translation="MAPTDRVHLPFRWSFIPQQDARDGSIRWIWRAYTQSGDLVLECK
                     GPFETFTECMNDARTQGYGGK"
     gene            2444..4348
                     /locus_tag="VFB53_08265"
     CDS             2444..4348
                     /locus_tag="VFB53_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN86763.1"
                     /translation="MAGNSSLVDPHLYQLLVEQAKDYALFVLDTDGRILTWNLGAQRL
                     KGYAGEEIVGRHFSVFYTREALDSGWPQHELKVAAAEGRFEDEGWRVRKDGSRFWANV
                     VITALRDPAGKLLGYSKITRDLTVRKLHEEALRQSEERFRLLIEGVVDYAIFMLDTEG
                     IVTSWNAGAERIKGYGRDEIVGRHFSRFYLEEDIEAGKPWEELANARNHGRTESEGWR
                     VRKDGKRFWARAVVSALYDDAGHLRGYAKVTQDLSDRRHLQDLEQATRHMNEFIATLA
                     HELRNPLAPIRAAVQVMAKAGPNDPAQEQLRQTIDRQSAQLARIVNDMIDISKITRGA
                     ISIERGPVDMADVARRAVEAAAPLIQSAKHTLEVEVPPEPLCVDGDIYRLTQIATNLL
                     NNAARYTPPGGTIWLSVGADASAATLRVRDTGRGIEPELMQRIFDMFVRGRAPLQRVG
                     GGLGIGLALSRKLAELHGGTLEASSGGPGQGSEFTLRLPLPEAHRRPGPPAPPPALRP
                     SVAQRVLVVDDNADAARMLELLLRSLGHETRIAHDGQEALRVAGVFRPQIVLLDIGMP
                     GLDGYEVARRLRAMRDGPSLRIVAITGWGQDADREKSRQAGFDVHLVKPVEPTELVRV
                     LDEKGGATLH"
     gene            complement(4355..6004)
                     /locus_tag="VFB53_08270"
     CDS             complement(4355..6004)
                     /locus_tag="VFB53_08270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZN86764.1"
                     /translation="MTRKPDLSRLALGAGLGLAGVALVEGLLLLGLQPLTPIGWPALG
                     TFAGAALGGVWGFAGGTLAIAVYYVINLLQPHRFPDFYGSAFNMVSWLVGLGAVGLAI
                     LAARPRVLRAAASEAELTALRAYEEALRDSEARLRMVTDNIPALVSYIDAQQRYRFNN
                     RAYEQWVRMPRDEIAGRKVREIWGEEMYARFKPNIERALRGERVSYEYSVTDAGVERH
                     VLASYVPDFDGAGRVKGFFVLGSDVTQLVTTRRELAAQRARLEAALDGSSVALWETDL
                     QNGRVYLSDAWAQMLGAPAGETVATMDELLALLHPDDVEAVRRASLEALRGPRGVYAV
                     EHRVRARNGEWMWVLSRGRVTERDPQSGRALRMIGTNYDITDRRRVEEAVQSAAQSDP
                     LTGLANRLLFTDRLRLALARSARTGSRLAVLYLDVDRFKEINDRLGHAAGDALLKDFA
                     ARLRASVRATDTVARFGGDEFVVLLDDVKEAGHAVRVAEKIVAECREPLRIEGHDVVA
                     TASIGLAYGAEGAEVETLLRRADTALYEAKSAGRNGYRVAP"
     gene            complement(6015..>6807)
                     /locus_tag="VFB53_08275"
     CDS             complement(6015..>6807)
                     /locus_tag="VFB53_08275"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN86765.1"
                     /translation="AGFGVQGYDVAPKARARLRQAGGKAVSSIAGIDAAVVITSLPSA
                     KALHDVSNELKGRRVVLETSTLPIEEKERARATLAKKGITLLDCPLSGTGAQARARDL
                     VVFASGERKAFERAKPFLPGFSRAHYYLGAFGNGSRMKFVANLLVAIHNVSTAEAFVL
                     GMKAGLAPKTIYEVMANSAGSSRMFQVRGPQMVAGRYGEATISVELFQKDMKIIGEFA
                     AALGAPTPLFNASAALYNAAMGMGHAREDTASVCAVLERMGGVRR"
BASE COUNT          991 a         2437 c         2438 g          941 t
ORIGIN      
        1 ccgactggct cgactacacg ctgagccgtc cggacggcgc gcgcatcgcg agctcgctgc
       61 cgccctcggt gagcgcgcag accgcgctcg cggaccgcga gagcttcgag ctcgcggtgc
      121 gcaacggcga gccggtggtg gggagcctcg cgcaatggcc gaacggccag aagtggggca
      181 ttccgctgcg catgccggtg gtggagaacg gggtggcgag gtacgtgctg accgcggtgc
      241 tgcgccccga cgcgttcacc gacttgctgc gcgcgcagcg ccttcccgag ggctggatcg
      301 cgggcatcgc cgaccggcac ggccggttca tcggccgcct gccgccggcc ccgcacgggc
      361 agctcgcctc cgagagctgg cgcgagcgca tgcagcgctc gccgcagggc tggttccgcg
      421 gccgcaccat cgagggacgc gagacctaca cgccgtacgt gacctccagc ctgagcggct
      481 gggcgctcgg catcgcgatt ccggtgcaga ccgtcgaggc ggcggcctgg cgcagcctgt
      541 ggctggtgct gatcatggtg ctgctcgcga gcggcatcgc ggtggggctc gccgtgctgc
      601 tcggctggcg catcgcccgg ccgatcgccg cgctcgcgca ggcggcggag gcggcggggc
      661 gcggcgagcg cgtcggcttt ccgctgcgca cgccgatcgc cgagctcgac tcgctctcgg
      721 ccgcgctcgc ccaggccgcg caggcgatgc acgacaagca cgagctcgcc gagcgcgaga
      781 aggccgcgct gcaggccgcg gatcgggcca aggacgaatt cctggccatg ctgtcgcacg
      841 agctgcgcaa cccgctcgcg gcgctcaccg ccgcggcgca cgtgctcagg cttgccgcgc
      901 cgggcgacgc cgccgcggtc caggcgcgcg ccgtggtcga ccggcagacc aagcacatgg
      961 cgcggctggt gggcgacctg ctcgacatca gccgcgttgc catgggcaag gtcgcgctcg
     1021 agcgcgagcg cttcgacctc gccgagtcgg cgaagaacgt gatcaacatg tggcgagcct
     1081 ccgggcgcct ggagcggcac cagctcgccc tggacatccg cccggcctgg atcgacgccg
     1141 accgcgcgcg catcgagcag atcctgtcga acctgctgga caacgcggtc aagttcacgc
     1201 ccgcggggcg cagcatcgcg gtgtcggtcg ggccgcaggg tgaatccgcg cggctgcagg
     1261 tgagcgacca gggcgagggg ctggcgccgg gctcggccga gcgcatgttc gatctcttcg
     1321 tgcaggggga gcgcggcctc gaccgcgccg ccggcggcct cggcgtcggg cttgcgctgg
     1381 tgaagcggct gagccagttg cacggcggca gcgtgtcggc gacgagcgcc ggcccgggaa
     1441 agggggcgac tttcacggtg ctgctgccgc tggtgcagcc ggccaccacg agatcgggtg
     1501 aggtggtgcg cgagccgctg ctcgcgccgc gccgcgttct gatcgtcgag gacaacgacg
     1561 acgcgcgcca gatgctgcac gaagcgctcg tttacggcgg gcacgaggtg cgagaggcgc
     1621 gcgacggcgc ctcgggcctc gcgctggccg cggaatcgcc gccggacgtt gcgctgatcg
     1681 acatcgggct gcccgacgtc gacggctacg aggtcgcgcg ccgcctgcgc gcatcgccgg
     1741 gcggccggcg catggggctg atcgcgatca ccggctacgg ccaggccgag gaccagcgcc
     1801 ttgcctacga ggcgggcttc gatgcccacc tcaccaagcc cgtggcgccg gagcgcctca
     1861 agcaggttat ggcaggcctg caataataac gggctgatga aacgcctcct cgtagtcgac
     1921 gacgacgccg acatgcgcct gacgctcaag ctcgcgctcg agctcgcggg ctacgccgtg
     1981 gaggtcgcgg ccaacggccg cgaggcgctg cacgcgcagc gccgccagcc cgccgacgtg
     2041 ctgatcacgg acatcttcat gcccgacgcc gacggcttcg aggcgatcga cgccttccgc
     2101 aaggagtttc cgcgcaccaa gatcgtggta gtctcgggcg gcgctcagtt ctcgaagcgc
     2161 gattaccttc ccgacgccga gctgatcggg gtcgacgcga cgctgcagaa gcccttcgac
     2221 gtcgacaccc tgctcgggct gatcaagacg ctgtaagcga tggcgcccac ggacagagtg
     2281 catctgccgt ttcgctggtc gttcatcccg cagcaggacg cgagggacgg ttcgatccgc
     2341 tggatctggc gcgcctacac gcagagcggc gacctggtgc tcgaatgcaa gggcccgttc
     2401 gaaaccttca cggagtgcat gaacgacgca agaacccagg ggtatggcgg gaaatagctc
     2461 cctcgtcgac ccgcacctct accagctgct ggtcgagcag gcgaaggatt acgccctgtt
     2521 cgtgctcgac acggacgggc gcatccttac ctggaacctc ggcgcgcagc gcctcaaggg
     2581 atacgccggc gaggagatcg tggggcggca tttctcggtg ttctacaccc gcgaggcgct
     2641 ggacagcggc tggccgcagc acgagctcaa ggtcgccgcg gccgaggggc gcttcgagga
     2701 cgaaggctgg cgcgtgcgca aggacggctc gcgcttctgg gcgaacgtgg tgatcaccgc
     2761 gctgcgcgac cccgccggca agctgctcgg ctattccaag atcacgcgcg acctcacggt
     2821 gcgcaagctg cacgaggagg cgttgcggca gagcgaggag cgcttccgcc tgctgatcga
     2881 gggggtggtg gactacgcga tcttcatgct cgacacggag ggcatcgtga ccagctggaa
     2941 cgcgggcgcc gagcgcatca agggatacgg ccgcgacgag atcgtcggca ggcacttctc
     3001 gcgcttctac ctcgaagagg acatcgaggc cggcaagccg tgggaggagc tggcgaacgc
     3061 gcgcaaccac gggcgcacgg agtccgaggg ctggcgcgtg cgcaaggacg gcaagcgttt
     3121 ctgggcgcgc gcggtggtga gcgcgcttta cgacgacgcc gggcacttgc gcggctacgc
     3181 caaggtaacg caggatctct ccgaccgacg ccacctgcag gacctcgagc aggcgacccg
     3241 gcacatgaac gagttcatcg ccacgctcgc gcacgagctg cgcaatccgc tggcgccgat
     3301 ccgcgccgcg gtgcaggtga tggccaaggc cgggccgaac gacccggcgc aggagcagct
     3361 gcgccagacg atcgaccggc agagcgcgca gctcgcgcgc atcgtcaacg acatgatcga
     3421 catctcgaag atcacgcgcg gcgcgatctc gatcgagcgc ggcccggtcg acatggccga
     3481 cgtcgcgcgc cgcgcggtgg aggcggccgc gccgctgatc cagtcggcga agcacacgct
     3541 ggaggtcgag gtgccgccgg agccgctctg cgtcgacggc gacatttacc gcctgacgca
     3601 gattgcgacc aacctgctga acaacgccgc gcgctacacg ccgcccggcg gcaccatctg
     3661 gctttcggtg ggcgcggacg cgagcgccgc cacgctgcgc gtgcgggaca ccggccgcgg
     3721 catcgagccg gaactgatgc agcgcatctt cgacatgttc gtgcgcggcc gcgcgccgct
     3781 gcagcgcgtg ggcggcggcc tgggtatcgg cctcgcgctg tcgcgcaagc tcgccgagct
     3841 gcatggcggc acgctggagg cgagcagcgg gggaccgggg caggggagcg agttcacctt
     3901 gcggctgccg ctgcccgagg cgcaccggcg tcccgggccg cccgcgccgc ccccggcgct
     3961 gcggccctcg gtcgcgcagc gcgtgctggt ggtggacgac aacgcggatg cggcgcgcat
     4021 gctggagctg ctcctgcgct cgctcgggca cgagacccgc atcgcgcacg acggccagga
     4081 agcgctgcgc gtggcgggcg tgttccggcc gcagatcgtg ctgctcgaca tcggcatgcc
     4141 gggcctggac ggctacgagg tcgcgcgccg cctgcgcgcc atgcgcgacg gcccgtcgct
     4201 gcgcatcgtg gcgatcaccg gctgggggca ggacgccgac cgggagaaat cacgccaggc
     4261 cggcttcgac gtccacctgg tgaagccggt ggagcctacc gagctggtcc gcgtcctgga
     4321 cgaaaaaggc ggcgccaccc tgcactgagc gcgatcacgg cgctacgcgg taaccgttgc
     4381 gcccggcgct tttcgcctcg tacagcgcgg tgtcggcccg gcggagcagg gtctccactt
     4441 ccgcgccttc ggcgccatac gccagcccga tgctcgcggt cgcgaccacg tcgtgcccct
     4501 cgatgcgcag cggctcgcgg cattccgcca cgatcttctc ggcgacccgc accgcgtggc
     4561 cggcctcctt cacgtcgtcg agcagcacca cgaactcgtc gccgccgaag cgcgccacgg
     4621 tatcggtggc gcgcacgctg gcgcgcagcc gcgccgcgaa gtccttgagc agcgcgtcgc
     4681 ccgcggcgtg gcccagccgg tcgttgatct ccttgaagcg gtcgacgtcg aggtagagca
     4741 ccgcgagtcg cgacccggtg cgcgcgctgc gcgcgagcgc gagccgcagc cgatcggtga
     4801 acagcagccg gttggcgagt cccgtgagcg ggtcggactg cgccgccgac tgcaccgcct
     4861 cctcgacgcg ccggcggtcg gtgatgtcgt agttggtgcc gatcatgcgc agcgcccgcc
     4921 cgctctgggg gtcgcgctcg gtgacgcggc cgcgcgacag cacccacatc cactcgccgt
     4981 tgcgcgcgcg cacgcgatgc tccaccgcgt acacgccgcg cggcccccgc agcgcttcca
     5041 gcgaggcgcg cctcaccgcc tcgacgtcgt ccgggtgcag cagcgcgagc agctcgtcca
     5101 tcgtggcgac ggtctcgccc gcgggcgcgc cgagcatctg cgcccacgcg tcgctcaggt
     5161 agacccggcc gttctgcagg tcggtctccc acaacgcgac gctcgagccg tccagcgccg
     5221 cctccaggcg cgcgcgctgc gccgcgagct cgcggcgcgt cgtgacgagc tgcgtgacgt
     5281 cgctgccgag cacgaaaaac cccttcacgc gcccggcgcc gtcgaagtcg ggcacgtagc
     5341 tcgccagcac gtggcgctcg acgccggcgt cggtcaccga gtactcgtac gagacgcgct
     5401 cgccgcgcag cgcgcgctcg atgttcggct tgaaccgggc gtacatctcc tcaccccaga
     5461 tctcgcgcac cttgcgcccc gcgatctcgt cgcgcggcat gcgcacccat tgctcgtagg
     5521 cccggttgtt gaagcggtag cgctgctggg cgtcgatata ggacacgagc gccgggatgt
     5581 tgtcggtgac catgcgcagg cgcgcttcgc tgtcgcgcag cgcctcctcg taggcgcgca
     5641 gcgccgtgag ctcggcctcg ctcgccgcgg cgcgcagcac gcgggggcgc gccgcgagta
     5701 tcgcgagccc gaccgcgccg agcccgacca gccacgacac catgttgaac gcgctgccat
     5761 agaaatccgg gaagcggtgc ggctgcagca ggttgatgac gtagtacacg gctatcgcga
     5821 gcgtgccgcc ggcgaagccc cacacgccgc cgagcgccgc gccggcaaaa gtgccgagcg
     5881 ccggccagcc gatcggcgtc agcggctgca ggccgaggag gagcagcccc tccaccagcg
     5941 cgacgccggc gaggccgagg cccgcgccga gcgcaaggcg ggacaggtcc ggcttgcggg
     6001 tcatgcgcgc gccgtcagcg ccgcacgccg cccatgcgct cgagcacggc gcatacgctt
     6061 gcggtgtcct cgcgcgcgtg gcccatgccc atcgccgcgt tgtagagcgc cgccgatgca
     6121 ttgaagagcg gcgtcggcgc gccgagcgcg gcggcgaatt cgccgatgat cttcatgtcc
     6181 ttctggaaca gctccacgct gatcgtcgcc tcgccgtagc ggcccgccac catctgcggc
     6241 ccgcgcacct ggaacatgcg cgagctgccc gcgctgttcg ccatgacctc gtagatcgtc
     6301 ttcggcgcga gccccgcctt catgccgagc acgaaagctt cggcggtcga gacgttgtgg
     6361 atcgccacca gcaggttggc gacgaacttc atccggctgc cgttgccgaa cgcaccgagg
     6421 tagtaatgcg ctcgggaaaa gcccggcagg aagggcttcg cccgctcgaa ggccttgcgc
     6481 tcgccgctcg cgaacaccac caggtcacgg gcgcgtgcct gcgccccggt gccggaaagc
     6541 gggcagtcga ggagcgtgat gcctttcttc gccagcgtcg cgcgcgctct ctctttctcc
     6601 tcgatcggca gcgtgctcgt ctcgagcacc acgcgcctgc ctttcaattc attgcttacg
     6661 tcgtgcagcg ccttggcgct gggcagtgag gtgatcacca ccgccgcgtc gatgcccgcg
     6721 atggacgata ccgccttgcc gccggcctgc ctgagccgcg cgcgcgcctt gggcgcgacg
     6781 tcgtatccct ggacgccgaa gccggcc
//
LOCUS       DASRIE010000197        21596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3304479, whole genome shotgun sequence.
ACCESSION   DASRIE010000197 DASRIE010000000
VERSION     DASRIE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304479"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFB53_08280"
     CDS             <1..621
                     /locus_tag="VFB53_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZN86766.1"
                     /translation="KQIEARTSLEAIDLYGLSEVIGPGVAQECAETKDGLTIWEDHFY
                     PEIVHPETGAVLPEGERGELVFTSLTKEAMPVIRYRTRDLTRLLPPTARSMRRMEKIT
                     GRSDDMLIIRGVNVFPTQIEELILKQKELAPHYVMEVSKDGPLDHLTVLVELAPGAAD
                     AGSAAKLQHLIKSYVGVSADVKLVPISTIERSIGKAKRVVDRRPKA"
     gene            complement(618..1097)
                     /locus_tag="VFB53_08285"
     CDS             complement(618..1097)
                     /locus_tag="VFB53_08285"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN86767.1"
                     /translation="MTTYDYFKSPQGRMLLVADGKGLAHVGFIGQKYAPRIAADWHRD
                     AAHPLLRKAKRELGEYFSGKRQRFSVKLAPRGTPFQRSVWKAISGVGFGRTIAYAELA
                     RRAGRPGSARAAGAATGRNPIGVIVPCHRIVGADGSLTGYAGGLAKKKALLALEARA"
     gene            complement(1094..2545)
                     /locus_tag="VFB53_08290"
     CDS             complement(1094..2545)
                     /locus_tag="VFB53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HZN86768.1"
                     /translation="MTLDSATCYRALRARDARFDGRFFVAVSSTRIYCRPVCTVKPPK
                     RENCRFYPSAAAAESGGYRPCLRCRPELAPGNASVDAVSRLAQAAASLIEDRTLDADG
                     LDAVAARLGITDRHLRRAFGAEFGVSPVEFAQTQRLLLAKRLLTDTGLPVTEIAFASG
                     FGSLRRFNALFKQRYRLQPGQLRRHMRAATSPAADALNFELSFRPPYDWPAVGAFLGA
                     RAIAGVEMLEGGCYRRTVRIAQEGKEHLGWVEVALSAKKPALRVAVSPSLARVLPPVL
                     SRVKALMDLACNPAEVAQALGALAKGHPGVRVPGAFDGFEVAVRAILGQQVSVAAART
                     VAGRFAEAFGDPVDTPFAALNRIFPAAERIAGLPYGRIARLGMPGARARTVLALARAV
                     ADGELDLVPNADIEATLDKLRALPGVGEWTAQYIAMRSLAWPDAFPHTDLGVMKALGM
                     KDAKHVLAAGEAWRPWRAYAVMHLWNSLAKDRP"
     gene            2656..3513
                     /locus_tag="VFB53_08295"
     CDS             2656..3513
                     /locus_tag="VFB53_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN86769.1"
                     /translation="MTPADYARLVALAALWGAAFIFMRIAAPVFGPAWTAELRVLLGG
                     LALLAWFRIAGFDPGLRAHWRFYLLMGTVGIAVPFVLYSFASIHAPASLLSITNSTAP
                     IFGLAWAALAGDERVTARKATGLALGVAGVALIARPDAAAAADPLFGWAVAAALGACC
                     LYGVVGVLIKRYARGATPRGMAAGNQLAAALVLIPLLPLLPPLAPPTPLAVANLLALA
                     LLASAAAFVLYFRLIADIGATRALTVTYLIPLFGILWGWLFLGEALPAAALAGGALIL
                     AGTVLVTRR"
     gene            3527..5524
                     /locus_tag="VFB53_08300"
     CDS             3527..5524
                     /locus_tag="VFB53_08300"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HZN86770.1"
                     /translation="MSAYPQLFRPLDLGFVTLPNRILMGSMHMGLEARPDGMERLAAF
                     YAERAKGGAALIVTGGFSPNGAGNLTTHRAQLSTAEDAARHRVIPRAVHAAGGRIVLQ
                     LLHAGRYGYHPQIVAPSPLKAPINQHAPRELPAAEIESTIADFASAAKLAREAGYDGV
                     ELMGSEGYLLTQFLVPRTNQRQDAWGGSLENRMRFPVEVVKAVRAAAGRDFIIVYRIS
                     VLELVEGGLSTKEMQQLARAVEAAGADILNSGIGWHEARIPTIAQAVPRGGFAWATRP
                     IKEAVKIPVIASNRINAPEVAEAILARGDADMVSLARAFLADEAFASKARAGDRAGIN
                     ICIACNQACLDHYFINQPASCVVNPRAGRETQLVFAPARKRKRVAVVGGGPAGLSCAA
                     VAAERGHEVVLFEKAAELGGQFNLAKRIPGKLEFGESVAYYAERLRRAGAKVLLDHAP
                     SEAELGQFDEVVLATGIDPRKPAIPGIDHRSVASYVDLLSGRVQAGRNVVIIGAGGIG
                     FDVALYLLERNGRATLDAKAFAQHWGIVQDPAVSGALDPRGLPPPHPAHHITMLKRSS
                     TPFGATLGRTTGWVHRAELGRNGVQSIKGVEYRRIDDAGVHVAVEGKERVIAADTVVI
                     CAGQEPLRPVAARHLIGGAREAGELDAKRAMLEGAELAAAL"
     gene            5526..6389
                     /locus_tag="VFB53_08305"
     CDS             5526..6389
                     /locus_tag="VFB53_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN86771.1"
                     /translation="MNAATLAGGAAVLLWSTLALLTAGATRIPPLQLLALSFAVASVV
                     GLLYAAARRVNLLRAARAHRGALLLAVAALGAYHLLYFVALANAPVVDASLIAYLWPL
                     LIVVIAAASGAAQVNRWHLAGVALGLAGAWLVIASRGALALSPRYALGYGAALACALV
                     WSAYSVANRRHAGAPSELVALACVAVTLLALVAHALLETTVLPSRREWLAILLLGAGP
                     AGAAFFLWDHGTKRGSLPLLGALSYAAPVLSTALLVASGDASPSWLLAASCALVAGGA
                     ALAALAERRLG"
     gene            6399..7127
                     /locus_tag="VFB53_08310"
     CDS             6399..7127
                     /locus_tag="VFB53_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN86772.1"
                     /translation="MDKAPVAVIAGVGEGLGFALARRFAAAGYRVALAARSAERLARL
                     AGEIGKAGGRAFAAPTDLREEQEVLALFDALESEHGPVEVAVFNAGANFRASLLDTPA
                     DMFEKVWRLGCYAGFLFGREAARRMAPRGKGTILFTGATASIRGAAQFAAFAAAKNGL
                     RAVAQSMAREFGPRNLHVAHVVIDGMIDTEAVRARFADRVADLGPDAMLDTAAIAELY
                     YQLHAQPRSAWTFEADLRPWAEKF"
     gene            7130..8071
                     /locus_tag="VFB53_08315"
     CDS             7130..8071
                     /locus_tag="VFB53_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN86773.1"
                     /translation="MAASDARRLFERIAANIEQVMRGQHAQVRKLLAAFAAGGHVLVD
                     DFPGTGKTTLAKSLAASIGARFARVQFTPDLLPSDILGVSVFNQRDQSFEFREGPVFT
                     CILLADEINRASPRTQSALLEVMAEGQVSVEGRTHPMDPLFFVVATQNPVEFRGTYPL
                     PEAQMDRFALCLALGYVDADTEVAVLNAQELGHPLESIGAVASREEVLALKEAARRVR
                     VADELKHYVVELVRRTRAAPQVLLGCGPRASIALTHTAKALALFDGDEFVRPAHVQEL
                     AVATLAHRMVLDPQARFAGVSAASLVGEILREVKVPA"
     gene            8068..9414
                     /locus_tag="VFB53_08320"
     CDS             8068..9414
                     /locus_tag="VFB53_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN86774.1"
                     /translation="MKRAVYAALRAVSALDHGLRQRLTPAGWLALGAGLAALAAGLDT
                     TQNASYQAAALLGSLLLLAWASSLAFRARVDIERELPRYATAGEPFTYAVAVANRGAR
                     PLEGATLVEALRDPRPKYADWKRAREPGEERRNWFDRNAGYFRWRWLIERRTPETPQE
                     AVLPALAPGARRTLRLALTPRRRGRIELAGLALARTDPLGLVKGLSRVPLPAQVIALP
                     RRYRLPPLALPGRRRHQPGGVTQSSSVGDTEEFLALREYRPGDPLQRIHWKSFARTGR
                     PIVKEFQDEFFERHALVLDTGGAHGEDAAFEDAVAVAASFVYTIDTQECLLDLLFVGG
                     AVRHYTAGRGQLQAEHMLEVLAGVGASAPQDFAALARVVLEQRAQLASCVLVLVGWDE
                     ARRRLAEQLAASGIEVRAILVCERARAPRAAPPWLLAVHPGAIEAGLAGLERRVLR"
     gene            9411..11333
                     /locus_tag="VFB53_08325"
     CDS             9411..11333
                     /locus_tag="VFB53_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZN86775.1"
                     /translation="MRLPPLIAGAALLAWGWHSGNLALAAPIALALEAPRWLRLRFAL
                     DAADYARIADLCTVFFVGLVVVLAANRGVAHGVIGAFQWAPVVLAPILLAQRLGEADR
                     VPLTALFRYLRKLKERQPELKVPMVDTGGVFVALCVICGGVGNPGAPGYYALAVLLAA
                     WALYPSRPRRASAAAWALLFAAGAGLGYAGHQGLSQLQHWVGGVIADWHLRRGEADPS
                     RGMTDIGTLGRLKQRDTIVARVTGKPQDVARARLLHRASYNAYVGTTWVGRRSTRGDL
                     DAEEGGVDWRLAPGPGAQRVQVALRTERRATLLALPPGVTRVTGLAATGALRTGLGVL
                     QATVDGDWIQYGAEFGPAIAYYDPPAVDDDQLPPQERETFAGLAHALGLAGLPAAAAV
                     ARVMRELSGFTYSIWRASPPPAGVTPLADFMTRTKSGHCEYFAAAATLLLRAAGVPAR
                     YATGYAVMEYSALEGAYVVRARHAHAWTRAFVDGRWIDLDPTPPDWFGEETNRLAPLW
                     EKVLDFFRWAAYRWSQREALEASDAWWGVLALLVAILAWRLLRGKRVRRPGASAAAGR
                     RYPGMDSEFYALTGDLPTRAEGEPLEAWLERVAPGKYGEALRLHQRYRFDPQGLEQAE
                     RARLRELCRAQPASSG"
     gene            complement(11658..11733)
                     /locus_tag="VFB53_08330"
     tRNA            complement(11658..11733)
                     /locus_tag="VFB53_08330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11698..11700),aa:Thr,seq:tgt)
     gene            complement(11763..11930)
                     /locus_tag="VFB53_08335"
     CDS             complement(11763..11930)
                     /locus_tag="VFB53_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86776.1"
                     /translation="MKRKSGAARATESLAEQRMREAAKEKGDNSFNFGSFLRKGSPQR
                     GKKRGRVYRIK"
     gene            complement(11958..12413)
                     /locus_tag="VFB53_08340"
     CDS             complement(11958..12413)
                     /locus_tag="VFB53_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HZN86777.1"
                     /translation="MSEISTKPYLLRALYEWCVDNGYTPHVAVKVDSKTQVPSEYVKN
                     GEITLNVAPSAVHKLQMGNELIEFSARFAGVARQISVPVGNVYAVYARETGHGMTFDV
                     DSAKPATQAVAEAEPAAPKDAPPALPAPANSAGEPPKKPGGKPTLRRVK"
     gene            complement(12418..13017)
                     /locus_tag="VFB53_08345"
     CDS             complement(12418..13017)
                     /locus_tag="VFB53_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZN86778.1"
                     /translation="MMQLYSGTTCPFSHRCRFVLYEKGMDFQVIDVDMYNKPEDLAVM
                     NPYNRLPVLVERDLILYESNIINEYIDERFPHPQLMPADPVMRARARLMLFNMEVELF
                     SQVEALESGKEKPMDKARQQVTDRLTELSPVFTRTKHMLGDDFTMLDVAIAPLLWRLD
                     HYGIKLGKPAAPLMKYAERIFSRPAFIEALTPSEKVMRK"
     gene            complement(13088..13732)
                     /locus_tag="VFB53_08350"
     CDS             complement(13088..13732)
                     /locus_tag="VFB53_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HZN86779.1"
                     /translation="MRKLLAALLFAPAALLAAGDGAPLEQAPIEPRDLVSLQSGARSF
                     VNYCLGCHGLQYMRYNRLTELGLTEAQIRDNLMFSADKVGETMKVAMTAKDGKAWFGV
                     PPPDLSVIARSRGADWLYGYLRGFYRDAKSAHGWNNKVYPNAAMPHVLWQVQAESSAV
                     QYDIAVRDLVNFLVYVGEPSAQSRKSIGIVVLFVLGVLFILAYAMKKAFWKDVR"
     gene            complement(13744..15012)
                     /locus_tag="VFB53_08355"
     CDS             complement(13744..15012)
                     /locus_tag="VFB53_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZN86780.1"
                     /translation="MALWDWFDARMPTVGEEWRKHAAEYYAPKNFNFWYYFGALSLLV
                     LVIQIVSGIFLTMHYKPDAALAFGSVEYIMRDVPGGWLIRYIHTTGASAFFIVVYLHM
                     FRALLYGSYKEPRELVWIFGVLIYLTLMGEAFFGYLLPWGQMSYWGAQVIVNLFGTVP
                     FVGETLALWIRGDYVVSDVTLNRFFALHVIALPLVLLGLVVAHILALHDVGSNNPDGV
                     EIKKNKDASGKPVDGIPFHPYYTVKDTVGAIVFLAVFCIVMFFMPELGGYFIEHNNYI
                     PADPLKTPEHIAPVWYFTPYYSILRAVPPLFNSQFPGVAAMGIATMILFLLPWLDRSP
                     VKSIRYRGAYWKSAVAIFVVVFLVLGYLGVKPTTVWGQFGFDAFFLDTKDRATWVARL
                     CTVLYFAFFILMPWYTARDKVKPVPDRVTM"
     gene            complement(15031..15621)
                     /gene="petA"
                     /locus_tag="VFB53_08360"
     CDS             complement(15031..15621)
                     /gene="petA"
                     /locus_tag="VFB53_08360"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZN86781.1"
                     /translation="MSDNQKVDKTRRNLVVATSVAGGAASVGAAVPFVASMFPSERAK
                     AAGAPIDVDISAIAPGELKVFEWRGKPVWVIRRTEDMLAALKKSAGLTDPASKSSQQP
                     DYASNEYRSLKPEVMVMEGVCTHLGCSPQIRTAEAKAEMGADWLGGFYCPCHGSKFDL
                     AGRVFRGAPAPTNLPVPPYQFLSDSTLRIGDDKKGA"
     gene            15747..16910
                     /locus_tag="VFB53_08365"
     CDS             15747..16910
                     /locus_tag="VFB53_08365"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZN86782.1"
                     /translation="MLRRLWLVFAQAATVCLAVLFVVSTLRPEWLPAGSPAPQVATVV
                     PVQTAVATAGPAPRPASSYHDAVVRATPSVVNIFTSKEAPRNPLLNDPTFRRFFGDQF
                     GDEAQRAASLGSGVIVSSAGYILTNHHVVEAAAEIEVALPDGKKLLAKLVGNDPETDL
                     AVLRVSAGKLPAITLGQSDALRVGDVVLAIGNPFGVGQTVTSGIVSALGRSGLHINTF
                     ENFIQTDAAINPGNSGGALVDGRGNLVGINTAIYSRSGGSMGIGFAIPVSTAKMVMEQ
                     IIRSGSVTRGWIGVEVQEITPALAESFKLPEPRGAIIAGVLRGGPADKAGVKPGDVLT
                     AINGAPVSDPTSMLNQVAALQPGASAQMKLRRQAQTLDLAITVGRRPKPQPRE"
     gene            16963..18153
                     /locus_tag="VFB53_08370"
     CDS             16963..18153
                     /locus_tag="VFB53_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN86783.1"
                     /translation="MKCISRWLVAPVLAAAAGLAFAQAKEIRIAHIYDKTGPLEAYAK
                     QSSNGLMMGLEYLTGGKMELLGRKIVVIEKDSQLKPDIGKAALAAAFGDDKADIAIGP
                     VGSGVALAMLPVAEEYKKILVVEPAVADSITGDKWNRYIFRTGRNSTQDAVAGAAALP
                     QDAVIGTLAQDYAFGRDGIKAFKDALAAVGSKAQIIHEEYAPQATTDFTAPAQRLFEA
                     LRGKPGRRIIVVVWAGAHPMAKLMDLKPERFGIEVAPGGNILPAMKGWKEFAGTEGAI
                     YYYYGFPKNKMNDWLVAEHQKRFGAPPDFFTAGGFAAASAVVAGIRKAGGTDTEKLIA
                     AMEGLSFDTPKGPMTFRKEDHQALQAMYHFRIKKAQKGEWDLLELVSEIPAAKLPIPI
                     RNRK"
     gene            18164..18922
                     /locus_tag="VFB53_08375"
     CDS             18164..18922
                     /locus_tag="VFB53_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86784.1"
                     /translation="MAHPVLETHGLTIRFGGHVAVDAVSCAFAPGTLTAIVGPNGAGK
                     TTYFNLISGQLKASAGRVSLYGEDISRLGAPQRTHRGIGRAFQLTNLFPRLTVLENVR
                     LAVQSRAGLGRDAWSMWGRHVELVEKAEHTLQRVGLADRREAVASALPHGDQRKLEVA
                     ILLALEPAVFMFDEPTAGMSVDEVPVILELIENIKAGGDKTVLLVEHKMDVVRSLADR
                     IIVLHQGALVADGDPAEVIASPIVQEAYLGVARA"
     gene            18927..19640
                     /locus_tag="VFB53_08380"
     CDS             18927..19640
                     /locus_tag="VFB53_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86785.1"
                     /translation="MLSLSGVNTHIGQYHILHGVDFAVERNSLTMLLGRNGAGKTTCL
                     RTIMGLWRASAGEVRYEGRDVTRLPTPDRARLGIAYVPENMGIFAGLTVQENLRLAAR
                     AGEPEAKRLAWITGLFPAMKTFRRSLAGNLSGGQKQMLAIARAIVEPRKLLLVDEPTK
                     GLAPAIIANMVEAFRELKRTDTTILVVEQNFHFARSLGDAVAVMDSGRIVHTGRMDAL
                     AADAAMQTRLLGLSLDSHQ"
     gene            19637..20611
                     /locus_tag="VFB53_08385"
     CDS             19637..20611
                     /locus_tag="VFB53_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86786.1"
                     /translation="MKADWLPLALVPLLALAALPLVGSVPTWVTLTVAGLAMGMMIFM
                     MASGLTLVFGLMDVLNFAHGIFISVGAYGAATVLTRMRPQMEAGSLAADLSAMIPAVL
                     AAIALAGLLGWFFERVLIRPVYGDHLKQILITMGGLIVFEQLIHVVWGADMIPLPRPS
                     AITGSFILGEAAIERYRVLAVIVGLAVFAAMWLLLNRTRIGLLVRAGVENPEMVESLG
                     YRIRRLFVGVFVAGSGLAGLGGAMWAMYQEQVTAQMGGQVLVMIFIVVIIGGLGSVGG
                     CFLGALLVGLMANYTGYLAPKLALGSNILLMVLVLLWRPRGLFPIGKA"
     gene            20617..>21596
                     /locus_tag="VFB53_08390"
     CDS             20617..>21596
                     /locus_tag="VFB53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86787.1"
                     /translation="MLARLLSNDTPRSPVLAVLLVAILVALALAPFVFPGTKSLNVAA
                     KVAVFVLLVASYDLLLGYTGIVSFAHTMFFGIGGYGVGIAMAKLGSGWGALALGIVAA
                     LLASLAIALAIGLLSLRVRAIFFAMITLAVAAFFQILASQLSAFTGGEDGLTFRLPEL
                     LRDRLTAYYAVLGVALALFLVLLRVVNSPFGRVLLSIRENEFRSEALGYRIVTYRTVA
                     NCLAALGATVAGVLYAVWLRYTGPDTTLSFNIMIDILLMVVIGGMGTMYGAVIGAALF
                     VVAQNYLQDLMRLASGATQGIPLLPGLLHPDRWLLWLGVLFILCVYYFPAG"
BASE COUNT         3050 a         7621 c         7650 g         3275 t
ORIGIN      
        1 aagcagatcg aggcgcgcac ctcgctcgag gcgatcgatc tctatggctt gtccgaagtg
       61 atcggcccgg gggtggcgca ggagtgcgcg gagaccaagg acggcctcac gatctgggag
      121 gaccatttct accccgagat cgtgcacccg gaaaccggcg cggtgctgcc cgagggcgag
      181 aggggcgaac tggtgttcac ttcgctcacc aaagaggcga tgccggtgat ccgctaccgc
      241 acgcgcgacc tcacgcgcct tctgccgccc accgcgcgca gcatgcgccg catggagaag
      301 atcaccgggc gttccgacga catgctgatc atccgcggcg tgaacgtgtt cccgacgcag
      361 atcgaggaac tgatcctcaa gcagaaggaa ctcgccccgc actacgtgat ggaagtgtcg
      421 aaggacggcc cgctggacca tctcacggtg ctggtggagc tcgcgcccgg cgcggccgac
      481 gccggctccg cagcgaagct gcagcacctg atcaagtcct acgtcggcgt gtcggcggac
      541 gtgaagctgg tccccatcag caccatcgag cgctcgatcg gcaaggcgaa gcgcgtggtg
      601 gacaggcgcc cgaaggccta ggcgcgggcc tccagcgcga ggagcgcctt tttcttcgcc
      661 agcccgccgg cgtagccggt gagcgagccg tccgcgccga cgatgcggtg gcagggcacg
      721 atgacgccga tcgggttcct gccggtcgcg gcccccgcgg cgcgcgcact gccgggccgg
      781 ccggcccgcc gcgccagctc cgcgtaggcg atggtgcggc cgaaaccgac ccccgagatg
      841 gccttccaca ccgagcgctg gaacggtgtg ccgcgcgggg cgagcttcac cgaaaagcgc
      901 tgacgcttgc ctgaaaagta ttcgcccagc tcgcgcttcg ccttgcgcag caacgggtgc
      961 gccgcatcgc ggtgccagtc ggcggcgatc cgcggtgcgt acttctgccc gatgaagccg
     1021 acatgggcca ggcctttgcc gtcagccacc agcagcatgc gcccctgcgg gctcttgaaa
     1081 tagtcgtagg tcgtcatggt cggtcctttg cgagtgagtt ccacaagtgc atcaccgcgt
     1141 acgcgcgcca gggccgccag gcttcgccgg cggccagcac gtgtttggcg tccttcatcc
     1201 cgagcgcctt catgacgccc aggtcggtgt ggggaaatgc atccggccag gcgagcgagc
     1261 gcatggcgat gtactgcgcg gtccattcgc ccacgcccgg cagcgcgcgc agcttgtcca
     1321 gggtcgcctc gatgtcggcg ttcggcacca ggtcgagctc gccgtccgcc acggcgcggg
     1381 cgagcgcgag caccgtgcgg gcgcgcgcgc ctggcatgcc gaggcgggcg atgcgcccgt
     1441 agggcagtcc ggcgatgcgc tccgcagccg ggaaaatcct gttcagtgcc gcgaagggcg
     1501 tgtccaccgg atcgccgaac gcctcggcga accggcccgc cacggtgcgc gcggcggcaa
     1561 cgctgacctg ctggccgagg atcgcgcgca ccgcgacctc gaagccgtca aaggcgccgg
     1621 gcacgcgcac accgggatgg cccttcgcca gtgcgcccag ggcttgcgcc acctcggccg
     1681 ggttgcaggc gaggtccatc agcgccttca cgcgcgagag caccggcggc agcacgcggg
     1741 cgagcgacgg ggacaccgcg acgcgcagcg cgggcttctt cgccgacagg gccacctcga
     1801 cccagcccag atgctccttg ccttcctgcg cgatgcgcac cgtcctgcgg tagcaaccgc
     1861 cttccagcat ctcgacgccg gcaatcgcgc gcgcgccgag gaaagcgccg accgcaggcc
     1921 agtcgtaggg cgggcggaag ctcaattcga agttcaaggc gtcggccgcg ggcgaggtcg
     1981 cggcgcgcat gtgtcggcgc agctgtcccg gctgcaggcg atagcgctgc ttgaacagcg
     2041 cgttgaagcg ccgcaggctg ccgaagccgc tggcgaacgc gatctcggtc accggcaggc
     2101 cggtgtcggt cagcaggcgc ttggcaagca gcaggcgctg ggtctgcgcg aactccaccg
     2161 gcgagacgcc gaactcggcg ccgaaggcgc gccgcaagtg ccggtcggtg atgccgaggc
     2221 gcgccgcgac ggcatccagg ccgtcggcgt ccagcgtgcg gtcctcgatc agacttgcgg
     2281 cggcctgcgc caggcgcgag accgcgtcca cgctggcatt gcccggcgcg agctcgggac
     2341 ggcagcgcag gcagggccgg tagccgcccg attcggccgc cgcggcgctg ggatagaagc
     2401 ggcagttctc gcgcttcggc ggcttcaccg tgcacacggg acggcagtag atgcgcgtcg
     2461 aggacacggc gacgaagaag cgcccgtcga agcgtgcgtc gcgcgcgcgc agtgcccggt
     2521 agcaggtggc ggagtcaagc gtcatggacg tgatggtggc gacaaaagcc aaagcctgct
     2581 cgccattttc ggactttact ccgcgacgtc cgaaaatggc gagcccgggg aaatgcaagg
     2641 acatacaatc gggtgatgac cccggccgac tatgcccgcc tggtggccct ggccgcgctc
     2701 tggggcgccg ccttcatctt catgcgcatc gccgcgccgg tgttcggccc ggcctggacc
     2761 gcggagttgc gcgtgctcct cgggggcctc gcgctgctcg cctggttccg catcgcgggc
     2821 ttcgatcccg ggctgcgcgc gcactggcgc ttctacctgc tgatgggcac ggtcggcatc
     2881 gcagtgccct tcgtcctcta ctccttcgcg tcgatccacg cgccggcctc gctgctgtcc
     2941 atcaccaact cgaccgcgcc catcttcggc ctcgcctggg ccgcgctcgc gggcgatgag
     3001 cgcgtcacgg cgcgcaaggc gacggggctg gcgctcggcg tggcaggcgt ggcgctgatc
     3061 gcgcgccccg acgccgccgc cgcggccgat cctctgttcg gctgggctgt cgcggcggcg
     3121 ctcggcgcct gctgcctgta cggcgtggtg ggcgtgctga tcaagcgcta cgcgcggggg
     3181 gcgacgccgc gcggcatggc ggccggcaac cagctcgcgg cggccctggt cctcattccg
     3241 ctgcttccgc tgctgccgcc ccttgcgccg ccgacgccgc tcgccgtcgc caacctgctg
     3301 gcgctcgcgc tgctggcgag cgcggccgcc ttcgtgctct atttccggct gatcgccgac
     3361 atcggcgcca cgcgcgcgct caccgtcact tacctgatcc cgctgttcgg catcctctgg
     3421 ggatggctgt tcctcggcga agcgctgccc gccgccgcgc tggccggcgg cgccctgatc
     3481 ctcgccggga cggtgctcgt cacccgcaga tagaattgcg ggccgcatga gcgcctaccc
     3541 gcagctgttc cgcccgctcg acctcggctt cgtcacgctg ccgaaccgca tcctcatggg
     3601 ctcgatgcac atggggctcg aggcgcggcc cgacggcatg gagcggctgg cggcgttcta
     3661 cgccgagcgc gcgaaaggcg gcgcggcgct gatcgtcacc ggcggcttct cgccgaacgg
     3721 cgcgggcaat ctcaccacgc accgcgcgca gctgagcacg gcagaggacg cggcgaggca
     3781 ccgagtcatc cctcgggcgg tgcatgccgc gggcgggcgc atcgtgctgc agctgctgca
     3841 cgccgggcgc tacggctacc acccgcagat cgtcgcgccc tcgccgctca aggcgccgat
     3901 caaccagcac gcgccgcgcg agctgcccgc cgccgagatc gagagcacca tcgccgattt
     3961 cgcctcggcg gcgaagctcg cgcgcgaggc gggctacgac ggcgtcgagc tgatgggctc
     4021 ggagggctac ctcctcaccc agttcctggt gccgcgcacc aaccagcgcc aggacgcctg
     4081 gggcgggtcg ctggagaacc gcatgcgctt ccccgtggaa gtggtgaagg cggtgcgcgc
     4141 cgcggccggc agggacttca tcatcgtcta ccgcatttcc gtgctcgaac tggtcgaggg
     4201 cggcttgtcc accaaggaga tgcagcagct cgcgcgcgcg gtcgaggcgg ccggcgccga
     4261 catcctcaac tccggcatcg gctggcacga agcgcgcatc ccgaccatcg cgcaggccgt
     4321 gccgcgcggc ggcttcgcct gggcgacgcg gccgatcaag gaggcggtga agatcccggt
     4381 gatcgcctcg aaccgcatca acgcccccga ggttgccgag gcgatcctcg cgcgcggcga
     4441 cgccgacatg gtgtccctgg cgcgcgcctt cctcgccgac gaggctttcg ccagcaaggc
     4501 cagggcgggc gaccgcgccg gcatcaacat ctgcatcgcc tgcaaccagg cctgcctgga
     4561 ccactacttc atcaaccagc cggcgagctg cgtggtcaac ccgcgcgccg ggcgcgagac
     4621 gcagctggtg ttcgcgccag cgcgcaagcg caagcgcgtc gcggtggtcg gcggcggccc
     4681 ggcgggactg tcctgcgcgg cggtcgccgc cgagcgcggc cacgaagtgg tgctgttcga
     4741 gaaggccgcc gagctcggcg ggcagttcaa ccttgccaag cggattcccg gcaagctcga
     4801 gttcggcgaa tcggtcgcct actacgccga gcggctgcgc cgcgccggcg ccaaggtgct
     4861 gctcgaccat gcgccctcgg aggccgaact cgggcagttc gacgaggtgg tgctcgccac
     4921 cggcatcgat ccgcgcaagc ccgccatccc cggcatcgat caccggagcg tcgcgagcta
     4981 cgtggacctg ctctccggcc gcgtgcaggc cggtcggaac gtggtgatca tcggcgccgg
     5041 cgggatcggc ttcgacgtcg cgctgtacct cctcgagcgc aacgggcgtg cgacgctcga
     5101 cgccaaggcc ttcgcgcagc actggggcat cgtgcaggac cccgcggttt cgggcgcgct
     5161 cgaccccagg ggcctgccgc cgccgcatcc cgcgcaccac atcaccatgc tcaagcgctc
     5221 gagcaccccg ttcggcgcga cgcttggccg caccaccggc tgggtgcacc gcgcggagct
     5281 cgggcgcaac ggggtgcagt ccatcaaggg cgtggagtac cgccgcatcg acgacgccgg
     5341 cgtgcatgtc gcggtggagg gcaaggagcg cgtcattgcc gccgataccg tggtgatctg
     5401 cgccgggcag gagccgctgc gcccggtggc ggccaggcac ctgatcggcg gcgccaggga
     5461 agcaggcgag ctcgacgcca agcgcgccat gctggaaggc gcggagctgg ccgcggcgct
     5521 ctagcatgaa cgccgccacg ctcgccggcg gcgccgcggt cctgctctgg tcaacgctcg
     5581 cgctgctcac cgccggcgcc acgcgcatcc cgccgctgca gctgctcgcc ctcagcttcg
     5641 ccgtggcgag cgtcgtgggg ctgctctacg ccgccgcgcg ccgcgtgaac ctgttgcgcg
     5701 cggcgcgcgc gcaccgcggc gccctgctgc tcgccgtcgc cgcgctcggc gcataccacc
     5761 tgctctactt cgtggccctg gccaatgcgc cggtggtgga tgcgagcctc atcgcctatc
     5821 tgtggccgct gctcatcgtg gtgatcgccg cggcgagcgg cgcagcgcag gtcaaccgct
     5881 ggcacctcgc gggcgtcgcg ctcggcctgg cgggcgcctg gctggtcatc gcctcgcgcg
     5941 gcgcgctcgc gctgtcgccg cgctacgccc tcggctatgg cgcggcgctc gcctgcgccc
     6001 tcgtctggtc ggcatactcc gtggccaacc ggcgccacgc cggcgcgccc tcggaactgg
     6061 tggcgctcgc ctgcgtcgcg gtgacgctcc tcgcgctcgt cgcgcatgcg ctgttggaga
     6121 cgaccgtcct gccctcgcgg cgcgaatggc tcgccatcct gctgctcggc gccggcccgg
     6181 cgggcgcggc gttcttcctc tgggaccacg gcaccaagcg cggcagcctg ccgctgctcg
     6241 gcgcgctgtc ctatgccgcg ccggtgctct ctaccgcgct gctggtggcg agcggcgacg
     6301 catcgcccag ctggctgctg gcggcgagct gcgcgctggt ggcgggcggc gctgcgctgg
     6361 cggcgctggc cgaacggcgc ctgggctaac atccggccat ggacaaggcg ccggtcgcgg
     6421 tcatcgcggg cgtgggcgaa gggctgggtt tcgcgctggc gcggcgcttc gcggccgccg
     6481 gctaccgcgt ggcgctcgcc gcgcgcagcg ccgagcgcct cgcgcgcctc gccggggaga
     6541 tcggcaaggc gggcggcagg gccttcgccg cgcccaccga cctgcgcgag gagcaggagg
     6601 tgctggccct gttcgacgcg ctggaatccg agcacggccc ggtggaggtg gcggtgttca
     6661 acgccggcgc caacttccgc gcttcgctgc tggacacgcc cgccgacatg ttcgagaagg
     6721 tctggcgcct gggctgctac gccggattcc tcttcggccg cgaggcggcg cggcgcatgg
     6781 cgccgcgcgg caagggcacc atcctgttca ccggcgccac cgcctcgatc cgcggcgccg
     6841 cgcagttcgc cgccttcgcc gcggcgaaga acggcctgcg cgccgtggcg caatccatgg
     6901 cgcgcgagtt cgggccgagg aacctgcatg tcgcgcacgt cgtcatcgac ggcatgatcg
     6961 acaccgaggc ggtgcgcgcg cgcttcgccg accgggtggc ggacctcggg ccggacgcga
     7021 tgctggacac ggcggcgatc gccgagctct attaccagct ccacgcgcag ccgcgctcgg
     7081 cctggacctt cgaggccgac ctgcgtccct gggcggagaa gttctagccg tggcggcgag
     7141 cgacgcgcgg cgcctgttcg agcgcatcgc cgccaacatc gagcaggtga tgcgcggcca
     7201 gcatgcgcag gtgcgaaagc tcctggcggc gtttgccgcc ggcggccacg tgctggtgga
     7261 cgatttcccc ggcaccggca agaccaccct cgccaagagc ctcgccgcct cgatcggcgc
     7321 gcgcttcgcg cgcgtgcagt tcacccccga cctgctgccc tcggacatcc tcggcgtgtc
     7381 ggtgttcaac cagcgcgacc agagcttcga gttccgcgaa gggccggtgt tcacctgcat
     7441 cctgctcgcc gacgagatca accgcgcctc gccgcgcacg cagtcggcgc tgctcgaggt
     7501 gatggccgag ggccaggtgt cggtcgaagg ccgcacccac ccgatggacc cgctgttctt
     7561 cgtggtggcg acgcagaacc cggtcgaatt ccgcggcacc tacccgctgc ccgaggcgca
     7621 gatggaccgc ttcgccctgt gcctggcgct cggctacgtc gatgcggaca ccgaggtggc
     7681 ggtgctcaac gcccaggagc tgggccatcc gctggagtcg atcggcgccg tggcctcgcg
     7741 cgaggaggtg ctggcgctga aggaggcggc gcgccgggtg cgcgtcgccg acgagctcaa
     7801 gcactacgtg gtggagctgg tgcgccgcac ccgcgccgcg ccgcaggtgc tgctcggctg
     7861 cgggccgcgc gcctcgatcg ccctcacgca taccgccaag gcgctggcgc tgttcgacgg
     7921 cgatgagttc gtgcgccccg cgcacgtgca ggagctcgcg gtggcaacgc tcgcgcaccg
     7981 catggtgctc gacccgcagg cgcgcttcgc cggcgtctcg gccgcctcgc tggtgggcga
     8041 gatcctgcgc gaggtgaaag tccccgcgtg aagcgggcgg tctatgccgc gctgcgcgcc
     8101 gtcagcgcgc tcgatcacgg cctgcggcag cggctcacgc cagccgggtg gctggccctg
     8161 ggcgccgggc tcgccgcgct ggcggcgggg ctggatacca cgcagaacgc aagctaccag
     8221 gcggcggcgc tgctcggttc gctgctcctg ctcgcctggg cctcgagcct cgcgttccgc
     8281 gcgcgcgtcg acatcgagcg cgaactgccg cgctatgcca cggcgggcga gcccttcacc
     8341 tacgccgtcg cggtggccaa ccgcggcgcg cggccgctgg agggcgcgac cctggtggag
     8401 gcgctgcgcg acccgcgccc gaagtacgcg gactggaagc gcgcgcgcga gccgggcgag
     8461 gagcggcgca actggttcga ccgcaacgcg ggctatttcc gctggcgctg gctgatcgag
     8521 cggcgcacgc cggagacgcc gcaggaggcg gtgctgccgg cgctcgcacc gggagcgcgc
     8581 cgcacgctgc gcctcgcgct caccccgcgc cgccgcgggc gcatcgagct cgccggcctg
     8641 gcgctggcgc gtaccgaccc gctcggcctg gtaaaggggc tctcgcgagt gccgctgccg
     8701 gcgcaggtga tcgcgctgcc gcgccgctac cggctgccgc cgctggcgct ccccgggcgc
     8761 cgccggcacc agccgggcgg ggtgacgcag tcgagctcgg tgggcgacac cgaggagttt
     8821 ctcgcgctgc gcgagtaccg gcccggggac ccgctgcagc gcattcactg gaagagcttc
     8881 gcgcgcaccg ggcgcccgat cgtcaaggag ttccaggacg agttcttcga gcggcacgcg
     8941 ctggtgctgg acaccggcgg cgcgcacggc gaggacgcgg ccttcgagga cgcggtggcg
     9001 gtggcggcgt ccttcgtcta caccatcgac acgcaggaat gcctgctcga cctcctgttc
     9061 gtcggcggcg cggtgcgcca ctacacggcg ggccgcggcc agctgcaagc cgagcacatg
     9121 ctcgaagtgc tggccggcgt cggtgcgagc gcgccgcagg atttcgcggc gttggcgcgc
     9181 gtggtgctgg agcagcgcgc gcagctcgcc agctgcgtcc tggtgctggt gggctgggac
     9241 gaggcgcgcc gccgtctcgc cgagcagctg gcggcgagcg gcatcgaggt gcgcgccatc
     9301 ctggtgtgcg agcgcgcacg cgcgccgcgc gccgccccgc cctggctgct ggccgtgcac
     9361 ccgggggcga tcgaggccgg tctggcgggc ctcgagcgca gggtgctgcg gtgagactgc
     9421 cgccgctgat cgccggcgcc gcgctcctcg cctggggctg gcacagcggc aacctggcgc
     9481 tcgccgcgcc gatcgcgctc gcgctcgagg cgccgcgctg gctgcggctg cgcttcgcgc
     9541 tggacgccgc cgactacgcg cgcatcgccg acctgtgcac cgtgttcttc gtcggcctcg
     9601 tggtggtgct cgccgccaac cgcggcgtgg cgcacggcgt gatcggcgcc ttccagtggg
     9661 cccccgtggt gctcgcgccg atcctgctgg cgcagcgcct gggcgaggcg gaccgcgtgc
     9721 cgctcaccgc gctgttccgc tacctgcgca agctgaagga gcgccagcca gagctcaagg
     9781 tgccgatggt ggacaccggc ggcgtgttcg tcgccctgtg cgtgatctgc ggcggcgtcg
     9841 gcaaccccgg cgcgcccggc tattacgcgc tcgccgtgct gctggccgcc tgggcgctct
     9901 acccgtcgcg gccgcgccgc gcgagcgccg ccgcctgggc gctgctgttc gccgccggcg
     9961 caggactggg ctacgccggc caccaagggc tgtcccagct gcagcactgg gtgggcggcg
    10021 tgatcgccga ctggcacctg cgccgcggcg aggcggatcc atcgcgcggc atgaccgaca
    10081 tcggcacgct cgggcggctc aagcagcgcg acaccatcgt ggcgcgcgtc accggcaagc
    10141 cgcaggacgt ggcgcgcgcg cggctgctgc accgcgcgag ctacaacgct tacgtcggca
    10201 cgacctgggt ggggcgccgc tcgacgcgcg gcgacctgga cgccgaggaa ggcggcgtcg
    10261 actggaggct cgcgccaggt cccggagcgc agcgcgtgca ggtcgcgctg cgcaccgagc
    10321 gccgcgccac cctgctcgcg ctgccgccgg gcgtcacgcg ggtcaccggc ctggcggcga
    10381 ccggcgcgct gcgcaccggc ctgggggtgc tgcaggccac ggtggatggc gactggatcc
    10441 agtacggcgc cgagttcggc ccggccatcg cctactacga tccgccggcg gtggacgacg
    10501 accagctgcc gccgcaggag cgggagacct tcgcgggact ggcgcatgcg ctcggcctcg
    10561 ccggcctgcc agccgccgcg gccgtggcgc gggtgatgcg cgaactctcc ggcttcacct
    10621 actcgatctg gcgcgcgtcg ccgccgcccg cgggcgtgac gccgctcgcc gatttcatga
    10681 cgcgcacgaa gagcgggcac tgcgagtatt tcgccgccgc ggcgacgctg ctgctgcgcg
    10741 ccgccggcgt tccggcgcgc tacgccaccg gctacgcggt gatggagtac agcgccctcg
    10801 aaggcgccta cgtggtgcgc gcgcgccacg cccatgcctg gacgcgcgcc ttcgtcgacg
    10861 gccgctggat cgacctcgac cccacgccgc cggactggtt cggcgaggaa acgaaccgcc
    10921 ttgcgccact ctgggagaaa gtgctggatt tcttccgctg ggcggcctac cgctggagcc
    10981 agcgcgaggc gctggaggcg agcgatgcct ggtggggcgt gctggcgctg ctggtcgcga
    11041 tcctcgcctg gcggctgttg cgcggcaagc gcgtgcggcg ccctggcgca agcgctgcgg
    11101 cggggcgccg ctatccgggg atggattcgg agttctacgc gctcaccggg gacctgccga
    11161 cgcgcgcgga gggcgagccg ctggaggcgt ggctggagcg cgtcgcgccc gggaaatacg
    11221 gcgaggcgct gcgcctgcac cagcgctacc gctttgaccc gcagggcctg gaacaggcgg
    11281 aacgcgcccg gctgagggaa ctgtgccggg cgcagcctgc ctcaagcgga tgagatttct
    11341 gctgctgctc gcctttgccc tgcccgcgtc cgcccaggag caggagatcc agcgggcgct
    11401 tatgcagcgc gaccagcagt cggcggagtt cgcctcgcgc ctgcgcggcg cgagcctcat
    11461 cgagcagcag cgcctggaaa acctctcggc gcgccagctg caggaagtgg cgaaggacat
    11521 gccgcaggag ctgcggccct acgaaaggca gaaggcggcc ggcgagcacg tcctcgccct
    11581 gccgccgccc gtcgtccacg tcaaggcgcc gaagaagccg gcgcccctgc ctgccaaatt
    11641 gccctgcgcg gtggaagtgg tgccggctgg aggaatcgaa ccaccgacca acggtttaca
    11701 aaaccgctgc tctaccaact gagctaagcc ggcccgaaaa tagggtcaga ccctattttc
    11761 cgctatttga tgcggtagac gcggccgcgc ttcttgccgc gctgcggcga gccctttctc
    11821 aggaacgagc cgaagttgaa gctgttgtcg cccttttcct tggcggcctc gcgcatgcgc
    11881 tgctcggcga gggattcggt ggcgcgcgcg gcgccggatt tgcgtttcat gatggctcca
    11941 gggttgcgtt gcgattccta cttgacccgg cgcagcgtcg gcttgccgcc gggcttcttc
    12001 ggcggctcgc cggcggagtt cgccggcgcc ggcagggcgg gcggcgcgtc cttcggcgcc
    12061 gccggctcgg cctccgccac cgcctgcgtc gccggcttgg cgctgtccac gtcgaaggtc
    12121 atgccgtgcc cggtttcgcg cgcgtacacg gcgtagacgt tgcccaccgg caccgagatc
    12181 tggcgcgcca cgccggcgaa gcgcgcggag aactcgatca gctcgttgcc catctgcagc
    12241 ttgtgcaccg ccgagggcgc gacgttgagc gtgatctcgc cgttcttcac gtattcggag
    12301 ggcacctggg tcttggagtc caccttcacc gccacatgcg gcgtgtagcc gttgtccacg
    12361 caccactcgt acagggcgcg cagcaggtag ggcttggtgg agatctcgct cacggcgcta
    12421 ctttcgcatc accttctcgg agggcgtcag cgcctcgatg aacgccgggc gcgagaagat
    12481 gcgctcggcg tacttcatca gcggcgccgc cggcttgccg agcttgatgc cgtagtggtc
    12541 caggcgccac agcagcggcg cgatcgccac gtccagcatg gtgaagtcgt cgcccagcat
    12601 gtgcttggtg cgcgtgaaca ccggcgagag ctcggtgagc cggtcggtga cctgctggcg
    12661 cgccttgtcc atcggctttt ccttgcccga ttcgagcgcc tcgacctggc tgaacagctc
    12721 cacttccatg ttgaacagca tgaggcgcgc ccgggcgcgc atcaccgggt ccgccggcat
    12781 cagctgcggg tgcggaaagc gctcgtcgat gtactcgttg atgatgttgg actcgtacag
    12841 gatcaggtcg cgctccacca gcaccggcag ccggttgtac gggttcatca ccgccaggtc
    12901 ctcgggcttg ttgtacatgt ccacgtcgat gacctggaag tccatgccct tctcgtagag
    12961 cacgaagcgg cagcggtggc tgaaggggca cgtggtgccg gaatacagtt gcatcatggg
    13021 gcggaggtcc tgatgaaaaa gccgggcggc aaaatgcgcc cggctcctgg tttggtgttg
    13081 cgcgggctta gcgcacgtcc ttccagaatg ccttcttcat ggcatacgcg aggatgaaca
    13141 gcacgccgag cacgaacagc acgacgatgc cgatcgactt gcgcgactgc gccgagggct
    13201 cgccgacgta gacgaggaag ttgaccaggt cgcgcaccgc gatgtcgtac tgcacggcgc
    13261 tggactcggc ctgcacctgc cacaacacgt gcggcatggc ggcgttgggg tagaccttgt
    13321 tgttccagcc gtgggcggac ttcgcgtcgc ggtagaagcc gcgcaggtag ccgtacagcc
    13381 agtccgcgcc gcgcgagcgc gcgatcaccg acaggtcggg cggcggcacg ccgaaccagg
    13441 ccttgccgtc cttcgccgtc atggcgacct tcatggtctc gccgaccttg tcggcgctga
    13501 acatcaggtt gtcgcggatc tgcgcctcgg tcaggccgag ctcggtgagg cggttgtagc
    13561 gcatgtactg caggccgtgg caaccgaggc agtagttgac gaagctgcgc gccccggact
    13621 gcaggctcac caggtcgcgc ggctcgatcg gcgcctgctc cagcggcgcg ccgtcgccgg
    13681 cggcgagcag cgcggcgggg gcgaacagga gggcagccag cagttttctc acggtggcgg
    13741 ccctcacatc gtcacccggt cggggaccgg cttcaccttg tccctggccg tgtaccaggg
    13801 catcaggatg aagaaggcga aatacagcac cgtgcacagg cgcgctaccc aggtggcgcg
    13861 gtccttggtg tcgaggaaaa acgcgtcgaa gccgaactgg ccccacaccg tggtcggctt
    13921 cacgccgagg tagcccagca ccaggaacac caccacgaag atcgccaccg cgctcttcca
    13981 gtaggcgccg cggtagcgga tcgacttcac cggcgagcgg tcgagccagg gcagcaggaa
    14041 caggatcatg gtggcgatgc ccatcgccgc cacgcccggg aactgcgaat tgaacagcgg
    14101 cggcaccgcg cgcaggatcg agtagtaggg cgtgaagtac cacaccgggg cgatgtgctc
    14161 gggcgtcttc agcggatcgg ccggaatgta gttgttgtgc tcgatgaaat agccgcccag
    14221 ttccggcatg aagaacatga cgatgcagaa cacggcgagg aacacgatgg cgcccacggt
    14281 gtccttcacc gtgtagtagg ggtggaaggg gatgccgtcc accggcttgc cgctggcatc
    14341 cttgttcttc ttgatctcga cgccgtcggg gttgttggag cccacgtcgt gcagcgcgag
    14401 gatgtgcgcc accaccaggc cgagcagcac cagcggcagc gcgatcacgt gcagcgcgaa
    14461 gaagcggttc agggtcacgt cggacaccac gtagtcgccg cggatccaca gcgccagcgt
    14521 ctcgccgaca aagggaacgg tgccgaacag gttcacgatc acctgcgcgc cccagtagga
    14581 catctggccc cagggcagga ggtagccgaa gaaggcttcg cccatcagcg tgaggtagat
    14641 cagcacgccg aagatccaca ccagctcccg cggctccttg taggagccgt acagcagcgc
    14701 gcggaacatg tgcaggtaca ccacgatgaa gaaggccgac gctccggtgg tatggatgta
    14761 gcggatcagc cagccgccgg gcacgtcgcg catgatgtac tcgaccgagc cgaaggcgag
    14821 cgcggcgtcg ggcttgtagt gcatggtcag gaagatgccc gagacgatct ggatcaccag
    14881 caccagcagc gacagcgcgc cgaagtagta ccagaagttg aaattcttcg gcgcgtagta
    14941 ctcggcggcg tgcttgcgcc actcctcgcc caccgtgggc atgcgcgcat cgaaccagtc
    15001 ccacagggcc atcaggcggg aggccatcgt tcaggctccc ttcttgtcgt cgccgatccg
    15061 cagcgtgctg tcggacagga actggtaggg cggcaccggc aggttggtcg gcgccggcgc
    15121 gccgcggaac acgcgcccgg ccaggtcgaa cttggagccg tggcaggggc agtagaaacc
    15181 gcccagccag tcggcgccca tctcggcttt ggcttccgcg gtgcggatct gcggcgagca
    15241 gcccaggtgc gtgcacacgc cttccatcac catcacttcc ggcttgagcg agcggtattc
    15301 attggacgcg taatcgggct gctgcgagga cttcgacgcc ggatccgtga ggccggcgga
    15361 tttcttcagc gccgccagca tgtcctcggt gcgccggatc acccacaccg gcttgccgcg
    15421 ccactcgaac accttcagct cgcccggcgc gatcgccgag atgtcgacgt cgatcggcgc
    15481 gcccgccgcc ttggcgcgtt ccgacggaaa catgctggcg acgaagggga ccgccgcccc
    15541 gaccgaggcc gcgccgcccg ccaccgaggt cgcgaccacg aggttgcggc gcgtcttgtc
    15601 gactttctgg ttatcgctca tggagttgat tcgaggctgg cgtgtgcgaa agaggcgcca
    15661 ttctagcgat cgagcgtcga aatgtccacg atttgccgcc taagcggttc aattttcagg
    15721 gttttgtgct aaattagccg ccccctatgc tgcgccgcct ctggttggtt tttgcgcagg
    15781 ccgcgaccgt ctgcctggcg gtgctgttcg tcgtttcgac gctgcggccc gagtggctgc
    15841 cggccggttc ccccgcgccg caggtcgcca ccgtcgtgcc ggtgcagacc gcggtcgcca
    15901 ccgccggccc cgcgccgcgg ccggcctcct cgtaccacga cgcggtggtt cgcgcgacgc
    15961 catcggtggt caacatcttc acgtcgaaag aagccccgcg caacccgctg ctcaacgatc
    16021 cgacgttccg gcgtttcttc ggcgaccagt tcggcgacga agcgcagcgc gccgccagcc
    16081 tgggctcggg cgtcatcgtc agttccgccg gctacatcct caccaaccac cacgtggtcg
    16141 aggccgccgc cgagatcgag gtcgcgctgc ccgacggcaa gaagctgctc gccaagctgg
    16201 tgggcaacga cccggagacc gacctcgcgg tgctgcgcgt gagcgccggc aagctgccgg
    16261 cgatcaccct cgggcaatcc gacgcgctgc gcgtcggcga cgtggtgctg gcaatcggca
    16321 atccattcgg cgtcggccag acggtgacct cgggcatcgt ttccgcgctg ggccgcagcg
    16381 gcctgcacat caacaccttc gagaatttca tccagaccga cgccgccatc aacccgggga
    16441 actcgggcgg cgccctggtg gacgggcgcg gcaacctggt gggcatcaac accgcgatct
    16501 actcgcgctc gggcggctcg atgggcatcg gcttcgcgat tcccgtgtcc acggccaaga
    16561 tggtgatgga gcagatcatc cgcagcggct cggtgacgcg cggctggatc ggggtggaag
    16621 tgcaggagat cacgccggcg ctggccgaat ccttcaagct gcccgagccg cgcggcgcga
    16681 tcatcgccgg ggtgctgcgc ggcggcccgg cggacaaggc gggcgtcaag cccggcgacg
    16741 tgctcacggc gatcaacggc gcgccggtca gcgatccgac cagcatgctc aaccaggtgg
    16801 ccgcgctgca gccgggcgcg agcgcgcaga tgaagctgcg gcgccaggcg cagaccctcg
    16861 acctggcgat caccgtgggc cgccggccga aaccgcaacc gcgcgagtag aaaatggaag
    16921 aaaatggggt ctgaccccat tttcgttctt cgaggagaga gcatgaaatg catttcgcgt
    16981 tggctcgtcg cgcccgttct ggccgcggcc gccggcctcg ccttcgccca ggcgaaggaa
    17041 atcaggatcg cgcacatcta cgacaagacc gggccgctgg aggcctacgc caagcagagc
    17101 agcaacgggc tgatgatggg cctggagtac ctcaccggcg gcaagatgga gctgctcggc
    17161 cgcaagatcg tggtcatcga gaaggactcg cagctgaagc ccgacatcgg caaggccgcc
    17221 ctcgccgccg cgttcggcga cgacaaggcg gacatcgcca tcgggccggt gggctcgggc
    17281 gtcgccctcg ccatgctgcc ggtggccgag gagtacaaga agatcctggt ggtggagccc
    17341 gcggtcgcgg actcgatcac gggcgacaag tggaaccggt acatcttccg taccggccgc
    17401 aattccacgc aggacgcggt ggccggcgcc gccgcgctgc cgcaggacgc cgtgatcggc
    17461 acgctggcgc aggactacgc cttcggccgc gacggcatca aggccttcaa ggatgcgctc
    17521 gccgccgtcg gctccaaggc gcagatcatc cacgaggagt acgcgccgca ggccaccacc
    17581 gacttcacgg cgcccgcgca gcgcctgttc gaggccctgc gcggcaagcc gggccgcagg
    17641 atcatcgtgg tggtatgggc gggcgcgcac ccgatggcga agctgatgga cctcaagccc
    17701 gagcgcttcg gcatcgaggt ggctcccggc ggcaacatcc tgccggcgat gaagggctgg
    17761 aaggaattcg ccggcaccga gggcgccatc tattactact acggcttccc gaagaacaag
    17821 atgaacgact ggctggtcgc cgagcaccag aagcgcttcg gcgcgccgcc ggacttcttc
    17881 accgcgggcg gcttcgcggc ggcgagcgcg gtcgtggcgg gcatccgcaa ggccggcggc
    17941 accgacaccg agaaactgat cgcggcgatg gaagggttgt cgttcgacac ccccaagggc
    18001 ccgatgacct tccgcaagga agaccaccag gcgctgcagg cgatgtacca cttccgcatc
    18061 aagaaggcgc agaaaggcga atgggatctg ctcgagctgg tgagcgagat cccggccgcg
    18121 aagctgccga tcccgatccg caacaggaag taagggctcc ggggtggcac accccgtcct
    18181 ggagacgcac ggcctgacga tccgcttcgg cgggcacgtc gcggtggatg cggtgtcctg
    18241 cgccttcgcg ccgggcaccc tcaccgcgat cgtcgggccg aacggcgcgg gcaagaccac
    18301 gtatttcaac ctgatctccg gacagctgaa ggcgagcgcg ggacgcgtct cgctctacgg
    18361 cgaggacatc agccggctcg gcgcgccgca gcgcacgcac cgcggcatcg gccgcgcgtt
    18421 ccagctcacc aacctgttcc cgcgcctcac ggtgctggag aacgtgcggc tcgcggtgca
    18481 gtcgcgtgcc ggcctgggac gcgacgcctg gtcgatgtgg ggccgccacg tggagctcgt
    18541 cgagaaggcc gagcacacgc tgcagcgcgt cggcctggcc gaccggcgcg aggccgtcgc
    18601 ctcggcgctg ccgcacggcg accagcgcaa gctcgaggtg gcgatcctgc tcgcgctcga
    18661 gccggcggtg ttcatgttcg acgaacccac cgcgggcatg agcgtggacg aggtgccggt
    18721 gatcctcgag ctgatcgaga acatcaaggc cgggggcgac aagacggtgc tcctggtcga
    18781 gcacaagatg gacgtggtgc gctcgctcgc cgaccgcatc atcgtgctgc accagggcgc
    18841 gctggtggcc gacggcgacc cggccgaggt gatcgcctcg ccgatcgttc aggaagccta
    18901 cctgggagtg gcgcgtgcct gaaccgatgc tctcgctgtc gggagtcaac acccatatcg
    18961 ggcagtacca catcctgcac ggcgtggact tcgcggtgga gcgcaattcc ctgaccatgc
    19021 tcctgggccg caacggcgcc ggcaagacca cctgcctgcg caccatcatg ggcctgtggc
    19081 gcgcctccgc gggcgaggtc cgctacgagg gccgcgacgt cacccggttg ccgacgcccg
    19141 accgcgcgcg cctgggcatc gcctacgtgc cggagaacat gggcatcttc gccggcctca
    19201 cggtgcagga gaacctgcgc ctcgccgcgc gcgccggcga gccggaggcg aagcgcctgg
    19261 cgtggatcac cggcctgttt ccggcgatga agaccttccg gcgctcgctc gccggcaatc
    19321 tttccggcgg ccagaagcag atgctcgcca tcgcgcgcgc catcgtcgag ccgcgcaagc
    19381 tcctcctggt ggacgagccg accaagggcc tcgcgcccgc gatcatcgcc aacatggtcg
    19441 aggcgttccg cgaactgaag cgcaccgata ccacgatcct ggtggtggag cagaacttcc
    19501 acttcgcccg gtcgctcggc gacgcggtcg cggtgatgga ctccggccgc atcgtgcaca
    19561 cgggccgcat ggacgcgctg gccgccgacg ccgcgatgca gacgcgcctg ctcggcctgt
    19621 cgctggactc gcaccagtga aggccgactg gctgcccctc gccctcgtcc cgctgctcgc
    19681 gctggcggcc ctgccgctgg tcggcagcgt gccgacctgg gtgacgctga cggtggcggg
    19741 cctggccatg gggatgatga tcttcatgat ggcctccggc ctcacgctgg tgttcggcct
    19801 gatggacgtg ctgaacttcg cgcacggcat cttcatctcg gtgggcgcct acggcgcggc
    19861 cacggtgctc acgcgcatgc ggccgcagat ggaagccggc agcctcgccg ccgacctctc
    19921 ggcgatgatt cccgcggtgc tggccgcgat cgcgctcgcc ggcctgctcg gctggttctt
    19981 cgagcgcgtc ctcatccggc cggtgtacgg cgaccacctg aagcagatcc tcatcaccat
    20041 gggcggcctc atcgtgttcg agcagctgat ccacgtggtg tggggcgcgg acatgatccc
    20101 gctgccgcgg cccagcgcca tcaccggctc gttcatcctg ggcgaagccg cgatcgagcg
    20161 ctaccgcgtg ctcgcggtga tcgtggggct cgccgtgttc gcggcgatgt ggctgctgct
    20221 gaaccgcacg cgcatcgggc tgctggtgcg cgccggggtc gagaacccgg aaatggtgga
    20281 atcgctcggc taccgcatcc ggcgcctgtt cgtcggcgtg ttcgtcgccg gctcggggct
    20341 cgcgggcctg ggcggcgcga tgtgggccat gtaccaggag caggtcaccg cgcagatggg
    20401 cggccaggtg ctggtgatga tcttcatcgt ggtgatcatc ggcgggctgg gctcggtcgg
    20461 cgggtgcttc ctcggcgcgc tgctggtggg gctgatggcc aactacacgg gctacctcgc
    20521 gcccaagctc gcgctcggct cgaacatcct gctgatggtg ctggtgctgc tgtggcgccc
    20581 gcgcggcctg tttccgatcg gcaaggcctg aggccgatgc tcgcgcgcct gctctccaac
    20641 gacaccccgc gcagccccgt gctcgcggtc ctgctggtcg cgatcctcgt cgccctggcg
    20701 ctggcgccct tcgtcttccc gggcaccaag tcgctgaacg tggccgccaa ggtcgcggtg
    20761 ttcgtgctgc tggtggcgag ctacgacctg ctgctcggct acacgggcat cgtgtccttc
    20821 gcgcacacca tgttcttcgg catcggcggc tacggcgtgg gcatcgccat ggcgaagctg
    20881 ggcagcggct ggggcgcgct cgcgctgggc atcgtcgcgg cgctccttgc gtccctggcc
    20941 atcgcgctcg ccatcgggct gctgtccctg cgcgtgcgcg cgatcttctt cgccatgatc
    21001 accctcgcgg tggcggcctt cttccagatc ctcgcctcgc agctctccgc cttcaccggc
    21061 ggcgaggacg ggctcacctt ccggctgccg gagctgctgc gcgaccgcct caccgcctac
    21121 tacgcggtgc tcggcgtcgc gctggcgctg ttcctcgtcc tcctgcgcgt ggtgaactcc
    21181 ccgttcgggc gcgtcctgct ctcgatccgc gagaacgaat tccgctccga ggcactgggc
    21241 taccgcatcg tcacctaccg cacggtggcc aactgcctcg ccgcgctggg cgcgacggtg
    21301 gccggcgtgc tgtacgcggt gtggctgcgc tacaccgggc cggacaccac gctgtcgttc
    21361 aacatcatga tcgacatcct gctgatggtg gtgatcggcg gcatgggcac gatgtacggc
    21421 gcggtgatcg gcgccgcgct gttcgtcgtg gcgcagaact acctgcagga cctgatgcgg
    21481 ctcgcgagcg gcgcgacgca gggcatcccg ctgctgcccg gcctgctgca cccggaccgc
    21541 tggctgctgt ggctgggcgt gctgttcatc ctgtgcgtgt actacttccc cgccgg
//
LOCUS       DASRIE010000198         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2524400, whole genome shotgun sequence.
ACCESSION   DASRIE010000198 DASRIE010000000
VERSION     DASRIE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524400"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFB53_08395"
     CDS             <1..432
                     /locus_tag="VFB53_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HZN86788.1"
                     /translation="PAAVLGLGATITTTKGTHAADKFFKGLYETALKPGEIITAVSFP
                     IPKRAAYTKFKNQASRFAIVGVFVADTAAGVRVAVTGAGPCALRLAEMEKALAAKFAP
                     ESVAGLKIKPDGLNSDLHASAEYRAHLITVMARRAVEAAMK"
     gene            478..1401
                     /locus_tag="VFB53_08400"
     CDS             478..1401
                     /locus_tag="VFB53_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN86789.1"
                     /translation="MPTPLPASLPSSIDDTLQLLTSADYVADRSLATALYLSLALRRP
                     LFLEGEAGTGKTEIAKVLASALGRELIRLQCYEGLDIAQAAYEWNYPRQMIEIRLAEA
                     SGERSKDKMSHDIFDNRFLIKRPLALALEGKPGMAPVLLIDELDRTDEPFEAYLLEVL
                     SDWQITVPEIGTIRAAEPPIVVITSNRTREIHDAVKRRCFYHWVDYPDARRELEILRR
                     KAPKSSAALSREVVAFVQRLRGVDLFKLPGVAETIDWANCLVALDKLALDPETVNNTL
                     GVLLKYQDDIERIAGEEAARLVGEAKSAATA"
     gene            1402..>2536
                     /locus_tag="VFB53_08405"
     CDS             1402..>2536
                     /locus_tag="VFB53_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN86790.1"
                     /translation="MLPQLEPGAPRGKLSENVMHFARLLRGAGLRVGPDRVVDCVRAL
                     EIAGAERRDDWYWTMSAVLLSREEQRPVFDQAFRLFWRDPRLTEKMLQLMLPKTYGRT
                     PKPEEQQSQRLTDALFGQKKPDDSREERKVEMDARLSFSAREVLQRMDFDTMSAAELA
                     EAKRMIRQLRLPLPLVRTRRHAPDLRGRRVHLRATLRESLREGGDVIPLVRSRPVEIH
                     PPLVVLCDISGSMNPYARMFLHFLHALTNDRDRVHTFVFGTRLTNVTRQLRHRDVDVA
                     MTRVADAIKDWSGGTRIGACLREFNWRWARRLLGQNACLLLVSDGLDREAGEGLGEEM
                     ERLAKSCRYLVWLNPLLRYDKFEARPAGVRAMLPHVDLFLPVHN"
BASE COUNT          404 a          903 c          867 g          362 t
ORIGIN      
        1 ccggcggcgg tcctcggcct gggggcgacc atcaccacca ccaagggcac gcacgcggcc
       61 gacaagttct tcaagggcct gtacgagacc gcgctcaagc ccggcgaaat catcaccgcg
      121 gtgtccttcc ccatccccaa gcgcgccgcc tacaccaagt tcaagaacca ggcgtcgcgc
      181 ttcgccatcg tcggggtgtt cgtggccgac accgccgccg gggtgcgcgt ggcggtgacc
      241 ggcgcggggc cctgcgcgct gcgcctcgcc gagatggaaa aggcgctcgc cgccaagttc
      301 gcgcccgagt cggtcgccgg cctgaagatc aagcccgacg ggctgaacag cgacctgcac
      361 gcctcggccg agtaccgcgc ccacctgatc acggtgatgg cgcggcgcgc ggtcgaggca
      421 gcgatgaaat agaatagcgc cacgcccgga caagcccgcc tcgagcgggc ttttttcatg
      481 cccacacccc tgcccgcctc tctcccctcc tcgatcgacg acaccctcca gctgctgacc
      541 tcggccgact acgtcgccga ccgcagcctg gccaccgcgc tgtacctgtc gctcgcgctg
      601 cggcggccgc tgttcctcga gggcgaggcc ggcaccggca agaccgagat cgccaaggtg
      661 ctcgccagcg cgctggggcg cgagctgatc cgcctgcagt gctacgaggg cctggacatc
      721 gcgcaggccg cctacgagtg gaactacccg cggcagatga tcgagatccg gctggccgag
      781 gcgtcggggg aaagatcgaa ggacaagatg tcgcacgaca tcttcgacaa ccgcttcctc
      841 atcaagcgac cgttggcgtt ggcgctggag ggcaagcccg gcatggcgcc ggtgctgctg
      901 atcgacgagc tggaccgcac cgacgagccg ttcgaggcct acctgctgga agtgctctcc
      961 gactggcaga tcacggtgcc cgagatcggc accatccgcg ccgcggagcc gccgatcgtg
     1021 gtcatcacct cgaatcgcac gcgcgaaatc cacgatgcgg tgaagcggcg ctgcttctac
     1081 cactgggtgg attatccgga tgcgcgccgc gagctcgaga tcctcaggcg caaggcgccg
     1141 aaatcctcgg ccgccctgtc gcgcgaagtg gtggccttcg tgcagcgcct gcgcggcgtc
     1201 gacctgttca agcttcccgg ggtggcggag accatcgact gggccaactg cctggtggcg
     1261 ctcgacaagc tggcgctcga cccggagacg gtgaacaaca ccctcggggt gctgctcaag
     1321 taccaggacg acatcgagag aatcgccggc gaggaggccg cgcgcctggt cggcgaagcc
     1381 aagagcgccg ccaccgccta gatgctgccg cagctcgagc cgggcgcccc gcgcggcaag
     1441 ctgtccgaga acgtgatgca cttcgcgcga ctgctgcgcg gcgcgggcct gcgcgtcggc
     1501 ccggaccgcg tggtggactg cgtgcgcgcg ctggagatcg caggcgccga gcgccgcgac
     1561 gactggtact ggaccatgtc ggcggtgctg ctgtcgcgcg aggagcagcg cccggtgttc
     1621 gaccaggcgt ttcgcctgtt ctggcgcgat cccaggctca ccgagaagat gctgcagctg
     1681 atgctgccca agacctacgg ccgcacgccc aagcccgaag agcagcagag ccagcgcctg
     1741 accgacgccc tgttcggcca gaagaagccc gacgacagcc gcgaggagcg caaggtggag
     1801 atggacgcgc gcttgagctt ctcggcgcgc gaggtgctgc agcgcatgga cttcgacacc
     1861 atgtcggccg cggagctggc cgaggcgaag aggatgatcc ggcagctgcg cctgccgctg
     1921 ccgctcgtgc gcacgcggcg ccacgcgccc gacctgcgcg gcaggcgcgt gcacctgcgc
     1981 gccaccttgc gcgaaagcct gcgcgagggc ggcgacgtga tcccgctggt gcgctccagg
     2041 cccgtcgaga tccatccgcc gctggtggtg ctgtgcgaca tctccggctc gatgaacccc
     2101 tacgcgcgca tgttcctgca cttcctgcac gcgctgacca acgaccgcga ccgcgtgcac
     2161 accttcgtgt tcggcacccg cctcaccaac gtcacgcgcc agctgcgcca ccgcgacgtc
     2221 gacgtggcga tgacgcgggt ggccgacgcc atcaaggact ggtcgggcgg cacgcgcatc
     2281 ggcgcctgcc tgcgcgagtt caactggcgc tgggcgcggc gcctgctcgg gcagaacgcc
     2341 tgcctgctgc tggtgtcgga cggcctggac cgcgaggcgg gcgagggcct cggcgaggag
     2401 atggagcggc tggcgaaatc ctgccgctac ctggtgtggc tcaacccgct cctgcgctac
     2461 gacaagttcg aggcgcggcc ggccggcgtg cgcgcgatgc tgccgcacgt cgatctcttc
     2521 ctgccggtgc ataatc
//
LOCUS       DASRIE010000199         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1511670, whole genome shotgun sequence.
ACCESSION   DASRIE010000199 DASRIE010000000
VERSION     DASRIE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511670"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFB53_08410"
     CDS             complement(<1..218)
                     /locus_tag="VFB53_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86791.1"
                     /translation="MTLNTTLAALGLALAAAAPLAALAQSKPDPAKTAAKEKARMQEL
                     QKQVRSYRCVTKAGRKFYGSTVPPQCAG"
     gene            283..972
                     /locus_tag="VFB53_08415"
     CDS             283..972
                     /locus_tag="VFB53_08415"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZN86792.1"
                     /translation="MHSADRAVIAETTAKMLLEVKAVLFSEDQPFIFTSGWASPVYID
                     CRKLIFYPRIRTQLVDFATATLARDAGFEQFDVVAGGETAGIPYAAWIADRMGLPMQY
                     IRKKPKGFGRNAQIEGDIREGARTLLVEDLTTDGRSKINFCKALREAGAVVDHVFVNF
                     YYDIFPESKKVMRELGVRLHHLATWWDVLRVVKQGNYLQAAQVAQVEKFLNAPAEWSA
                     AHGGVSSFPSS"
     gene            969..1745
                     /locus_tag="VFB53_08420"
     CDS             969..1745
                     /locus_tag="VFB53_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN86793.1"
                     /translation="MRVITLNVNGIRSAAKKGLYPWLAAQKADVVCLQEIKAQEADLG
                     AEVLAPKGLYGHFYPAEKKGYAGVALYSRAKPDRVVKGFGAKEFDAEGRYLEASFGTL
                     KVVSLYLPSGSAGEHRQASKFRFLEAFVPQLKKLARSGNEVLLCGDWNIAHREIDLKN
                     WRSNQKNSGFLPEERAWLSRVLDELGWVDVFRRLNQKPEQYTWWSSRGQAWAKNVGWR
                     IDYQVGTPGIAARAAKESIYKRRRFSDHAPLTIDYDRPLG"
     gene            1726..3024
                     /locus_tag="VFB53_08425"
     CDS             1726..3024
                     /locus_tag="VFB53_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN86794.1"
                     /translation="MTGRWASALQPYLERAPLAALFLGISSGFAFAMIGATLTTRLAQ
                     HGISKGAVTAFALTFLAYNLKFLWAPLVDHVRLPLLGRFGQRRSWLWFSGALVMAAVA
                     FLGAADPRESLALVAAAAIAVGFAGATFDIIIDAYRIELLEPRQLGAGSGMSQYGWRI
                     GAAAAGGLALVLAARFGWAAAYAACAAFALPAMLVGVFAGEPARHLAPARPRGLIGAV
                     AAYVSPLVEFFRRQGALVVLLFVLVHKIGDTLANLTLRLLFQDLGFTNDEVAFYDVGI
                     GFAALLAGILAGGVLYARLGMKRSVLVSLVLMAVSNLSFAGLAAAGHSNAGMAAAVGF
                     ENFASGIGGVTVVAYLSALCNLRFTATQYALLSALASIAGRFLTGTTAGGLIDAMGYV
                     NFYLLTTLVALPGVALYWYMLRAGLADVPAGAAGAPPATA"
     gene            3062..>3291
                     /locus_tag="VFB53_08430"
     CDS             3062..>3291
                     /locus_tag="VFB53_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86795.1"
                     /translation="MRRRQLGLGWFGTLIVLALAVGAGWYAYTEVFGDDDAPPPSCAA
                     AQNACLQKCRRSSTEAPAAQSCQQACQREAEA"
BASE COUNT          445 a         1146 c         1155 g          545 t
ORIGIN      
        1 tcgccggcgc actgcggcgg caccgtggag ccgtagaact tgcgccccgc cttggtgacg
       61 cagcggtagg agcgcacctg cttctgcagc tcctgcatgc gcgccttctc cttggcggcc
      121 gtcttggccg ggtcgggctt ggactgcgcg agcgccgcga gcggcgcggc cgcggccagc
      181 gccaggccga gcgcggcgag cgtggtgttc agcgtcatcg tggcccccgt ggtcggtgca
      241 tcgcgcgaat tctaaccctg ctagaatccg cgcgcctccg ccatgcattc cgccgaccgc
      301 gccgtcatcg ccgagaccac cgccaagatg ctcctcgagg tgaaggcggt gctgttctcc
      361 gaggaccagc cgttcatctt cacttcgggc tgggcgagcc cggtgtacat cgactgcagg
      421 aagctcatct tctacccgcg catccgcacg cagctggtgg atttcgccac cgccacgctg
      481 gcgcgcgacg ccggcttcga gcagttcgac gtggtggcgg gcggcgagac cgcgggcatt
      541 ccctacgccg cctggatcgc cgaccgcatg ggcctgccga tgcagtacat ccgcaagaag
      601 ccgaagggct tcggccgcaa cgcgcagatc gagggcgaca tccgcgaagg cgcgcgcacg
      661 ctgctggtgg aggacctgac caccgacggg cgctcgaaga tcaatttctg caaggcgctg
      721 cgcgaggcgg gcgcggtggt ggatcacgtg ttcgtgaatt tctattacga catcttcccc
      781 gagtcgaaga aggtgatgcg cgagctcggc gtgcgcctgc accacctcgc cacctggtgg
      841 gacgtgctgc gcgtggtgaa gcagggcaac tatctgcagg ccgcgcaggt ggcgcaggtg
      901 gagaagttcc tcaacgcgcc ggccgagtgg tccgcggccc acggcggcgt ctccagcttt
      961 ccctcctctt gagggtaata accctcaacg tcaacggcat ccgctccgcg gcgaagaagg
     1021 gcctctaccc ctggctcgcc gcgcagaagg cggacgtggt ctgcctgcag gagatcaagg
     1081 cgcaggaagc cgacctcggt gcggaggtgc tcgcgcccaa agggctgtac ggccacttct
     1141 atccggcgga gaagaaaggc tacgccggcg tcgcgctgta ttcgcgcgcc aagccggacc
     1201 gcgtggtgaa ggggtttggt gctaaggagt tcgatgccga ggggcgctac ctcgaggcga
     1261 gcttcggcac gctgaaggtg gtgtcgctgt acctgccttc cggctccgcc ggcgagcacc
     1321 gccaggcctc gaagttccgc ttcctcgagg ccttcgtccc gcagctgaag aagctcgcgc
     1381 gctcgggaaa cgaggtgctg ctgtgcggcg actggaacat cgcgcaccgc gagatcgacc
     1441 tgaagaactg gcgctcgaac cagaagaatt caggtttcct gcccgaggag cgcgcctggc
     1501 tttcgcgcgt gctcgacgag ctgggctggg tggacgtgtt ccgccgcctc aaccagaagc
     1561 ccgagcagta cacctggtgg tccagccgcg gccaggcctg ggcgaagaac gtgggctggc
     1621 gcatcgacta ccaggtcggc acgccgggca tcgccgcgcg cgcggcgaag gaatcgatct
     1681 acaagcggcg gcgcttctcc gaccatgccc ccttgacgat cgactatgac cggccgctgg
     1741 gctagcgcgc tgcagcccta cctcgagcga gcgccgctcg cggcgctgtt cctcggcatc
     1801 tcctcgggct tcgccttcgc catgatcggg gcgacgctta ccacgcgcct cgcgcagcac
     1861 ggcatctcga agggcgcggt caccgccttc gcgcttacct tccttgccta caacctcaag
     1921 ttcctttggg cgccgctggt cgaccatgtc cgcctgccgc tcctcggccg gttcggccag
     1981 cgccgcagct ggctgtggtt ctcgggcgcg ctggtgatgg cagcggttgc gttcctcggc
     2041 gccgccgacc cgcgcgagtc gctcgcgctc gtcgccgccg ccgcgatcgc ggtgggcttc
     2101 gccggggcca ccttcgacat catcatcgac gcctaccgca tcgagctgct cgagccgcgc
     2161 caactcggcg ccggctccgg catgtcgcaa tacggctggc gcatcggcgc cgccgccgcc
     2221 gggggcctgg cgctggtgct cgccgcgcgc ttcggctggg cggccgccta cgccgcctgc
     2281 gcggccttcg ccctgccggc gatgctggtg ggcgtgttcg ccggcgagcc ggcgcgccat
     2341 ctggcgccgg cgcggccgcg cggcttgatc ggcgcggtgg cggcctatgt cagcccgctc
     2401 gtggagttct tccggcgcca aggcgcgctg gtcgtcctgc tgttcgtgct ggtgcacaag
     2461 atcggcgaca cgctcgccaa cctcacgctg cggctgctgt tccaggacct cggcttcacc
     2521 aacgacgaag tggccttcta cgacgtgggc atcggcttcg ccgcgctgct cgccgggatc
     2581 ctggcgggcg gggtgctcta tgcacggctg gggatgaagc gatcggtgct ggtgagcctg
     2641 gtgctgatgg cggtgtcgaa cctgagcttc gccggcctgg ccgccgcggg ccacagcaac
     2701 gccggcatgg ccgcggccgt cggtttcgag aatttcgcga gcggcatcgg cggggtgacg
     2761 gtggtcgcct acctgtcggc gctctgcaac ctgcgcttta ccgcgacgca gtacgcgctg
     2821 ctctcggcgc tggcgagcat cgccgggcgc ttcctcaccg gaaccacggc gggcgggctc
     2881 atcgacgcca tgggctacgt gaacttctac ctgctgacca cgctggtcgc gctgcccggc
     2941 gtcgcgctgt actggtatat gctgcgcgcg ggcctcgccg acgttccggc cggcgccgcc
     3001 ggcgcgcccc ctgcaacggc ttgatctcgc cgccgccggc cgcgggtcga ggaggtgcgt
     3061 gatgcgcagg cggcaactgg ggttgggctg gttcgggacc ctgatcgtgc tggcgctcgc
     3121 ggtgggggcg ggctggtacg cctacacgga agtcttcggc gacgacgacg cgccgccgcc
     3181 gagctgcgcc gcggcgcaaa acgcctgcct gcagaagtgc cggcgctcga gcaccgaggc
     3241 gcccgccgcg caaagctgcc agcaggcctg ccagcgcgag gcggaggcct g
//
LOCUS       DASRIE010000200        17399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1511778, whole genome shotgun sequence.
ACCESSION   DASRIE010000200 DASRIE010000000
VERSION     DASRIE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511778"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2984
                     /locus_tag="VFB53_08435"
     CDS             <1..2984
                     /locus_tag="VFB53_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN86796.1"
                     /translation="KAQLNVNNRAKQVEPRLPAEVRRQGLTVEKGSSAFLQVLAFYSP
                     DGRHDDIFISNYVTLNVLDEIKRVPGTTLVQIFGAKDYAMRIWLRPDRLATLKLAPSD
                     VIRAVNEQNAQFAAGKVGETPTAEPRELVYSVTTKGRLSEVREFEDIILRANGDGSLL
                     RLKDVARVELAGKDYSFLGKYNGRPATLMGVFLQPGANQLEVGEAVRKRITELSQRFP
                     AGFTYAIPFDTTMFVEVSIREVVITLLEAMLLVFLVVFLFLQNWRATLIPFAAVPVSL
                     IGTFAGLLLLGYSINTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEGLQPRDAAIKA
                     MKEVTSPIIAIVLVLCAVFIPIAFLGGLTGELYRQFAVTISIAVIISGFVALTLTPSL
                     CAIVLRPQHGERSQRWFGWFNRGFARVTDRYESGVAWLIRRGGVALLLFAGMVAVTGF
                     LWRTTPGSLVPDEDQGWYFAPVILPDGASLQRTDKMVEEVTAAIRSNPFNEHVMAFTG
                     FDFAGGFVFRENAATIFVTQTHWDKRPMPVGALVGEFYGKTAHLKEGLAIAFPPPAIF
                     GLGTAGGFEFYLQNRGEGGAKRLWEVSQQFIGAASQSPKIAMVNTFWRANVPQLYVDV
                     DREKAKSLGVPLDDVFSTLAATLGSYYVNDFNKYGRTWQVLMSAEPGQRASPEDAGAI
                     HVRSQKGDMIPLRSLARIEYTSGPGTLTRFNNLPAVQLLGGGRPGVSSGEAIAEIERI
                     AREVLPPEFSLDWSGASFQEKRSGGTSVLVLALGALMAFLILAAQYEKWSLPLSVMLA
                     LPFGTFGALAAIWINNTLGSLYHIVGGLAPGLQWLVAPLQGLARLSNDVYFQIGLVTL
                     LGLAAKNAILIVEYAVMKHHEGLSASAAAVEAARLRFRPILMTSLAFILGVLPLVVSD
                     GAGAGARNSAGTGVMGGMLAATFLAIFFVPTFFKLMTDRHLTEERSSAEIKAEAGQAR
                     TLAHRAVAGPRHAPHRGDADA"
     gene            2977..4353
                     /locus_tag="VFB53_08440"
     CDS             2977..4353
                     /locus_tag="VFB53_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZN86797.1"
                     /translation="MRSAALALLVAAAGCAVQPDYRPPDADLPQAWRESAPRPAGEAP
                     WWTVYGDAELERLMQEALAQNSDLLVAAARVDEARALLGEAQSGLRPQVQAGASAGRS
                     LSSAATGLLPPGTPRERDNYRATLDLSWEIDLFGRLRAAREAARAELAASEALRDGVR
                     LALTARVAASYYGLLALDAQVELTRRALALREESLALQRRRRAAGVISDYDLRQIEAE
                     AATLRAQLPPLERDREIEEAALAALLGRSPRAVFESALARSALAQAPAATAVPAGMPS
                     ELLLRRPDLVEAERRLAAANARVAAARAAYFPRISLTGLLGSESAELGDLFTGPAGIW
                     SFAAALAQPIYGGGRLAAQRDAAAARERAALAQYQGAVRNAFREVRQALAAQGRARES
                     FEAQAERVRALEETLRLARLRYASGIASQLDVLDAERGLLAAQTARVDALRAQRAAVA
                     DLYRALGG"
     gene            4422..5474
                     /locus_tag="VFB53_08445"
     CDS             4422..5474
                     /locus_tag="VFB53_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86798.1"
                     /translation="MADEVYALTERGKSELQGGATSCTPRELEILVLVDGKAGAGAVA
                     ARAKSVPAAEAPGVLQRLLQLGLIQKAGADAGQGYSFIGGGELLSSASREADAGTADL
                     RRNGYYVSIARRAAQARTPAAGKALSVLIVEDDPVLAKMLGHLMTLEGFAPQVAFRSG
                     EIAAAFGREPPPDLVLLDVVLPDEDGFAVLARMKGDARLRAIPVIMVTGRTTREGVMR
                     GLAGGADGYVTKPIQYEALANGVKAVLGLGVAGEPPAASAEQLGADDTLSELRREYRR
                     GLPLKLARIEELAKAGKLEELLRMAHSMAGAAPTLGLAPVGAAAKALEEALLAKDPEK
                     AARLVAALRAAAKAAA"
     gene            complement(5476..6216)
                     /locus_tag="VFB53_08450"
     CDS             complement(5476..6216)
                     /locus_tag="VFB53_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86799.1"
                     /translation="MDLGLAGKTALVTGASAGIGRGIALALAAEGVRLAVAARRRELL
                     ETLAKETGAQLVIIEADFLAEGAPQRIAEAALAGLGGVDILVNNAGGSRAFKLDAGEA
                     QWEEALTLNFTRQRQLTHALLGQMMARKWGRIVNITGKSEPEGINGAFAAKAAMHAWA
                     KGLSREVGKHGITVNSIPPGRILSEQILRNYTPEYRQWQSEHEIPVGEYGKPEDIANL
                     VCFLASPRARYITGAVIPVDGGLRRYQF"
     gene            complement(6216..7025)
                     /locus_tag="VFB53_08455"
     CDS             complement(6216..7025)
                     /locus_tag="VFB53_08455"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZN86800.1"
                     /translation="MQATGAALPTVFLSHGSPMHALEAGASGKAWAALGERLPRPRAL
                     LIASAHWEAEQPMLGGSARPETIHDFHGFPQPLYQIRYPSPGAPALALRAQALLRAAG
                     LQADIDPERGLDHGAWSPLLYAYPGADVPVLQVSVQTRLGPRHHLALGAALAPLAGEG
                     VLIIGSGHLTHNLRELRSLRDTGVGSAQPYVAEFQAWVRERIEAHELERLADYRRLSA
                     AGARAHPTEEHFLPLFAALGAAHADYRAERFVDHVEGGVLAMDAYLFHPAH"
     gene            complement(7032..7538)
                     /locus_tag="VFB53_08460"
     CDS             complement(7032..7538)
                     /locus_tag="VFB53_08460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN86801.1"
                     /translation="MSVHEVSIRLAAPRDAQAIALMSRDLVETGLGWKYDAARMLQAI
                     RAPDTLAPLACAGGRIAGFALMEFGEERAHLVLLAVRPAERRLGIGRRLVEWLVESAR
                     VAGIASLHLELRAANEAARGFYRALGFDETIVVPGYYRGREAALRMIRVLRATGPVPY
                     TWRPSSTI"
     gene            complement(7571..10126)
                     /locus_tag="VFB53_08465"
     CDS             complement(7571..10126)
                     /locus_tag="VFB53_08465"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZN86802.1"
                     /translation="MRARAALFLLIAALGAPAQAQAPPRLEPGVSQELARWRARHYRD
                     PRYDLRLRLDESQDAVAGRLELRVTLARRVDLVLDWRGAPVRALRVNGKPAKARHARE
                     HLVVPRAALRQGENRVALEFAARAAPAGSALTRYRDQEDGSSYLYSLFVPADASSAFP
                     CFDQPDLKARFTLELALPPGWRAVSNAPALEEAQGRARFAATAPISTYLFAFAAGPFE
                     VLEQPGEPVRLFVRRSQLERARAQAPEVLRLNRAALGYFAKEFARPYPFAKYDLVLIP
                     ELAYGGMEHAGATFLSEQAVLFPYAPSNPDLLRRAQLLFHEASHQWFGNLVTMRWFDD
                     LWLKEGFANFMAAKAAAALLPELEPWSAFHALKTGAARTDATAGTTPLRQPLANLSAA
                     KSAYGAIVYAKGPALLRQAEFYLGERAFRRALRDFLARHAWGAADWSDLVRAFERASR
                     RDLRAWAGTWVTRRGLPEISLRRSEGRLLLAQRDALGEGGLWAQKLVVAAAGTDGQIR
                     TSETRLESRQVRVVGPAAHPTARWLYANAGDFGYGRFLLDAQSRDALLAEPRALPDGL
                     LRAQLVEALWESVRDAALAPDAFIEFALALAPEEPDDVILAGLLGRVETAFRRYLSDA
                     QRERLAPRLERVLLDALLAEAPPSRRILLLRTYAALAWSGAALASLKQLLEGSLALPG
                     VELRVRDRFRLAERLVLRGDPDGPRALAQLAATERGDDARRYAFAASAALPDAEAKRR
                     MFERFAGDAGLPEGWVEEALAPFNAPEHAAYTQPLLGQALARLPELKRSRKIFFVEHW
                     LGAFLAGQSDARALEEVRRVLAGDLDADLRLKLLEAADGLQRSVRIRARHAGG"
     gene            complement(10140..10697)
                     /locus_tag="VFB53_08470"
     CDS             complement(10140..10697)
                     /locus_tag="VFB53_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86803.1"
                     /translation="MPSMHPQTPQQRLGQLLLGPLDDALGLARRYRDVDAFRDYVAER
                     WQLMIPAVLLVVVAAIACGLTPVLLLVGTQPAAALAGLLLAPVVLVGSLFVLVLVLFS
                     WLEERALARSLGHRTGPAQGPAARWLRKKLGADLGKAPRVPWLLAALFVIGPLLLLAT
                     RAPGVAVPLVILLVLAPIGFARLDR"
     gene            10804..12183
                     /locus_tag="VFB53_08475"
     CDS             10804..12183
                     /locus_tag="VFB53_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN86804.1"
                     /translation="MKPDFIVILIDDLRYDEFGAGGHPYMKTPQVDRLAAEGALFERA
                     FHTTPICSPNRASIVTGQYASRHGIIDNVARDAMSHRLPNYHLALQKLGYETGHVGKW
                     HMGNDGMPRPGYDHWVSFDGHGSLSDPRLNVNGAYRRRAGYMTDILNRAALEFVEQAR
                     RKPYALFFAHKAVHPDAHQAADGTLDMSRFGGYLPAERHRGLYRGAVFPRKPNMLSPR
                     RLVKQAPAWAEAFTLRGTARSRALLASIQAGTQEEIRQRARMMAAVDEGVGMLLASLA
                     RRGRLDDTLILFLGDNGYFFGEHGLGPERRFAYEEGIRAPFVLRYPRRVRAGSRPRDL
                     VICQDIAPTLIELAGGRPGAQVQGRSLLPLLSGRRRSWRKSFLIEYWAEQAMPWLVGM
                     TYKAVRTGRHKLIRWVNRGAGGELDELYDLERDPFELQNLARRASHARIRASLRRDLR
                     RLVADAAGL"
     gene            12207..13433
                     /locus_tag="VFB53_08480"
     CDS             12207..13433
                     /locus_tag="VFB53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZN86805.1"
                     /translation="MDSIDRPTIQRSATDIIAEVKLQSPPAAAERLAHFGGAEVAAVL
                     TRLSAGFAQEILGALPAESRERALAAAPLEFARQWQRNALYGEGTIGRMMEPVYAAFP
                     PQATVGQAIEALHELVKSAFITYIYVCDDDERLLGIVTMRDLLFNEHAKALRDVMLTE
                     VFALHAPVVLEDAMKQVLDRHYPVYPVVDAENRLRGLVRGQSMFEARAFDIAAQPGSM
                     VGVDKEERLGTPWQRSFFMRHPWLQLNLLTAFLAAAVVGYFQDTVDRLVILALFLPVL
                     AGQSGNTGCQALAVTLRGMTIGELKPGSQKALITKEAGLGLLNGAGVGVAAAIGMYVT
                     ASSQSSPNALMLSFVVFLAMIGACVASGLSGALVPLTLKRLGFDPATASSIFLTTATD
                     VVSMGMLLGLATILVR"
     gene            13468..13902
                     /locus_tag="VFB53_08485"
     CDS             13468..13902
                     /locus_tag="VFB53_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN86806.1"
                     /translation="MQVKEILRVKGNRLLSIEPSGRAVDAVRTMAEQNLGSLIVLDKE
                     RMVGVLTFREILQALAARAGALGDLRVSDVMVRDPVTATPDMEVNELRRTMLDSGARY
                     LPVVQDGKLVGVISFRDVAKAVLEEQDFENKMLKGYIKNWPA"
     gene            14069..15127
                     /locus_tag="VFB53_08490"
     CDS             14069..15127
                     /locus_tag="VFB53_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN86807.1"
                     /translation="MWDAHAHLVGTGDAGSGVVVNPRMESLLNPAEYARRLFFLNAGC
                     AHDAPGSVDRAYVERMRNLVAGLRPGAKLLLFAFERAHDERGAPQPERSGFFVPDAYA
                     RDTARAHPEAFEWAASIHPYRADCVAALERARAEGARAVKWLPAAMGMDPGSPLCDRF
                     YAALARLRLPLITHAGLERAVLGTDLQDYGNPLRLRRALDAGVRVVVAHCASMGEDRD
                     LDRGPHGPATDSFALFSRLMDEPRHARHLFGDISAVTQVNRAGPTLARLLERADWHPR
                     LLNGSDYPLPGLMPIFSVDYIVSLGLLEPAAAPVLGEIRAHNPLLFDFVLKRSLRLQG
                     RALASSVFETRRFFEGGV"
     gene            15138..16223
                     /gene="aroC"
                     /locus_tag="VFB53_08495"
     CDS             15138..16223
                     /gene="aroC"
                     /locus_tag="VFB53_08495"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZN86808.1"
                     /translation="MSGNTFGTLYCVTSFGESHGPAYGGVVDGCPPGLALEAADLQRE
                     LDRRKPGSSRHVTQRRETDTVEILSGVFEGKTTGTPIGFLVRNEDQRPKDYAEIARKF
                     RPGHADYSYWQKYGLRDPRGGGRASARETVVRVAAGAIARKWLAARYGVQVRGYLAQL
                     GPKKIPFQDWAAVAANPFFAPNAGMVAELEKFMDGLREAGDSCGARVNVVAHRVPVGW
                     GEPVYGRLDADIAAAMMSINAVKGVEIGAGFAAVEQKGSEHGDEMTPQGFAGNNNGGI
                     LGGISSGQDVTVSIALKPTSSIRLPRRTIDLEGKATSVQTTGRHDPCVGIRATPIAEA
                     MLACVLMDHALRHRAQCGDVTPPTRPV"
     gene            16282..16521
                     /gene="hfq"
                     /locus_tag="VFB53_08500"
     CDS             16282..16521
                     /gene="hfq"
                     /locus_tag="VFB53_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZN86809.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNSVTQMVYKHAISTVVPARAVSLGVEPDKDA"
     gene            16518..>17399
                     /gene="hflX"
                     /locus_tag="VFB53_08505"
     CDS             16518..>17399
                     /gene="hflX"
                     /locus_tag="VFB53_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZN86810.1"
                     /translation="MIPAAAPDNQAAVLVSLDIGTAAPEDAAEELLRLAESAGIRRHA
                     AVRGRRQRPDPKFYAGSGKVAQIGAAVAALNAGWAIFNHELSPAQERNLEAELNCRVL
                     DRTGLILEIFSQRAQTSEGKLQVELAQLERASTRLVRGWTHLERQRGGLGKTGGPGEK
                     QIELDRRYIARRVRVLRDRLDVLRRQRRVQRRSRVRREVLSVSLVGYTNAGKSTLFNA
                     LAHAGAYAADQLFATLDTTTRRVWLPEAGNVAVSDTVGFIRDLPHGLVSAFRATLEET
                     VHADLLLHVVDAASPVRE"
BASE COUNT         2438 a         6257 c         6383 g         2321 t
ORIGIN      
        1 acaaggcgca gctcaacgtc aacaaccgcg ccaagcaggt ggagccccgc ttgccggcgg
       61 aggtgcgccg ccagggcctg acggtggaga agggctcgtc ggccttcctc caggtgctgg
      121 cgttctattc gcccgacggg cgccacgacg acatcttcat ctccaactac gtgaccctca
      181 acgtgctgga cgagatcaag cgcgtgccgg ggaccacgct ggtgcagatc ttcggcgcca
      241 aggactacgc gatgcgcatc tggctgcggc ccgaccgcct cgccacgctc aagctcgcgc
      301 cgagcgacgt gatccgcgcg gtgaacgagc agaacgcgca gttcgccgcc ggcaaggtgg
      361 gcgagacgcc gaccgccgag ccgcgcgagc tggtctacag cgtcaccacg aaggggcgcc
      421 tgtcggaagt gcgggagttc gaggacatca tcctgcgcgc gaacggcgac ggttcgctcc
      481 tgcgcctgaa ggacgtggcg cgcgtcgagc tcgcgggcaa ggactacagc ttcctcggca
      541 agtacaacgg caggccggcg accctgatgg gcgtgttcct gcagccgggc gccaaccagc
      601 tcgaggtcgg cgaggcggtg cgcaagcgca tcaccgagct ctcgcagcgc ttccctgcgg
      661 gcttcaccta cgccattcct ttcgatacca ccatgttcgt cgaggtgtcg atccgcgagg
      721 tcgtgatcac gctgctggag gccatgctgc tggtgttcct ggtggtgttc ctgttcctgc
      781 agaactggcg cgccaccctc atcccgttcg ctgcggtccc ggtgtcgctg atcggcacct
      841 tcgcggggct gctgctgctc ggctactcca tcaacaccct gacgctgttc ggcatggtgc
      901 tcgccatcgg catcgtggtg gacgacgcca tcgtcgtgct ggagaacgtc gagcgcatca
      961 tgcacgagga gggcctccag ccgcgcgacg ccgcgatcaa ggccatgaag gaagtgacca
     1021 gcccgatcat cgccatcgtg ctggtgctgt gcgcggtttt catccccatc gccttcctcg
     1081 gcggcctgac cggcgagctg taccgccagt tcgcggtcac catctccatc gccgtcatca
     1141 tctcgggctt cgtcgcgctc acgctcaccc cgagcctgtg cgcgatcgtg ctgcggccgc
     1201 agcacggcga gcgctcccag cgctggttcg gctggttcaa ccgcggcttc gcgcgcgtca
     1261 ccgaccgcta cgagtcgggt gtcgcctggc tgatccggcg cggcggcgtg gcgctcctgc
     1321 tgttcgccgg catggtggcc gtcaccggct ttctctggcg caccaccccg ggctcgctgg
     1381 tccccgacga agaccagggc tggtacttcg cgccggtgat cctgccggac ggcgcgtcgc
     1441 tgcagcgcac cgacaagatg gtggaagagg tcaccgccgc gatccgctcc aacccgttca
     1501 acgagcacgt gatggccttc accggcttcg acttcgccgg cggcttcgtc ttccgcgaga
     1561 acgccgccac catcttcgtc acccagaccc actgggacaa gcggccgatg ccggtgggcg
     1621 cgctggtcgg cgagttctac ggcaagaccg cgcacctcaa ggaaggcctg gcgatcgctt
     1681 tcccgccgcc ggcgatcttc ggcctgggga ccgcgggcgg cttcgagttc tacctgcaga
     1741 accgcggtga aggcggcgcc aagcggctgt gggaggtgtc gcagcagttc atcggcgccg
     1801 cctcgcagag cccgaagatc gcgatggtca atacgttctg gcgcgccaac gtgccgcagc
     1861 tctacgtcga cgtcgaccgc gagaaggcca agagcctcgg cgtgccgctc gacgacgtct
     1921 tcagcacgct cgccgcgacc ctcggcagct actacgtcaa cgacttcaac aagtacggcc
     1981 gcacctggca ggtgctgatg tcggccgagc ccggccagcg cgcgagcccc gaggacgccg
     2041 gcgcgatcca tgtcagaagc caaaagggcg acatgatccc gctgcgctcg ctcgcgcgca
     2101 tcgagtacac gagcgggccg ggcacgctga cgcgcttcaa caacctgccg gcggtgcagc
     2161 tcctcggcgg cggccgcccc ggggtgagtt cgggcgaggc gatcgccgag atcgagcgca
     2221 tcgcgcgcga agtgctgccg ccggagttca gcctcgactg gagcggcgcg tcattccagg
     2281 agaagagatc gggcggcacc tccgtgctgg tgctggccct cggcgcgctg atggccttcc
     2341 tgatcctcgc ggcgcagtac gaaaagtggt cgctgccgct ctcggtgatg ctggcgctgc
     2401 cgttcggcac cttcggcgcc ctggcggcga tctggatcaa caacacgctc ggctcgctct
     2461 accacatcgt cggcggcctc gcgccagggc tgcaatggct ggtggcgccg ctccaagggc
     2521 tcgcccgcct gagcaacgac gtgtatttcc agatcggcct ggtgaccttg ctcggcctgg
     2581 cggcgaagaa cgccatcctg atcgtcgagt acgcggtcat gaagcatcac gaagggctgt
     2641 cggcttcggc tgccgcggtg gaggcggccc ggctgcgctt ccggccgatc ctcatgacgt
     2701 ccctggcgtt catcctcggc gtgctgccgc tggtggtgtc cgacggcgcg ggggcgggcg
     2761 cgcgcaactc cgccggcacc ggcgtgatgg gcgggatgct ggcggccacc ttcctcgcca
     2821 tcttcttcgt gccgacgttc ttcaagctga tgacggaccg ccacctcacc gaggagcgct
     2881 cctcggccga gatcaaggcc gaggcggggc aggcgcgcac gctcgcgcat cgcgcggtcg
     2941 cagggccgcg ccacgcgccg caccggggcg acgccgatgc gtagcgccgc gctcgcgctg
     3001 ctcgtcgccg cggccggctg cgcggtgcag ccggattacc ggccgccgga cgccgacctg
     3061 ccgcaggcgt ggcgcgagtc cgcgccgcgg cccgccggcg aggcgccctg gtggacggtc
     3121 tacggcgacg cggagctcga gcgcctgatg caggaggcgc tggcgcagaa cagcgacctg
     3181 ctggtcgccg cggcgcgcgt cgacgaggcg cgcgcgctgt tgggcgaggc gcagtccggc
     3241 ctgcgcccgc aggtacaggc gggcgcgagc gcgggccggt ccctgtcctc cgccgccacg
     3301 gggctgctgc cgccgggcac gccgcgcgag cgcgacaact accgcgccac gctcgacctg
     3361 tcctgggaaa tcgacctgtt cggccggctg cgcgcggcgc gcgaggcggc gcgcgccgag
     3421 ctcgcggcga gcgaggcgct gcgcgacggc gtgcgcctgg cgctcaccgc gcgcgtggcc
     3481 gcctcgtact acggcctgct ggcgctcgac gcgcaggtcg agctgacgcg gcgcgcgctc
     3541 gcgctgcgcg aggagtcgct cgcgctgcag cgccggcggc gcgcggccgg ggtgatctcg
     3601 gactacgacc tgcgccagat cgaggcggag gccgcgaccc tgcgcgcgca gctgccgccg
     3661 ctggagcgcg accgcgagat cgaggaggcg gcgctcgccg cgctgctcgg gcgctcgccg
     3721 cgcgcggtgt tcgagtcggc gctggcgcgc agcgcgctgg cgcaggcgcc ggcggcgacc
     3781 gcggtgccgg cgggcatgcc ttccgaactc ctgctgcgcc ggcccgacct ggtggaagcc
     3841 gagcggcgcc tcgcggcggc gaacgcgcgc gtggcggcgg cgcgcgcggc atacttcccc
     3901 aggatctcgc tcaccggcct gctcggcagc gaaagcgccg agctcggcga cctgttcacg
     3961 ggtcccgccg gcatctggag cttcgccgcc gcgctggcgc agccgatcta cggcggcggg
     4021 cggctggcgg cgcagcgcga cgcggccgcg gcgcgcgagc gcgcggcgct ggcgcagtac
     4081 cagggcgcgg tgcgcaacgc gttccgcgaa gtgcggcagg cgctggcggc gcaggggcgc
     4141 gcgcgggaga gcttcgaggc gcaggccgag cgcgtgcgcg cgctggagga aacgctgcgc
     4201 ctcgcgcgcc tgcgctacgc cagcggcatt gccagccagc tcgacgtgct cgacgccgag
     4261 cgcggcctgc tcgccgcgca gaccgcgcgc gtcgacgcgc tgcgcgcgca gcgcgccgcg
     4321 gtggccgacc tgtaccgggc cctcggcggc tagccccggc gccggcgcgc ggccggccgt
     4381 atactgccct ccggcggcgc atcctgcgga gggagctgcg catggcggac gaggtctacg
     4441 cgctcaccga gcgcggcaag tcggagctgc agggcggcgc gacgtcctgc acgccgcgcg
     4501 aactcgagat cctggtgctg gtggacggca aggccggcgc gggcgcagtc gcggcgcgcg
     4561 ccaagagcgt gccggcggca gaggcgcccg gcgtgctgca gcggctgttg cagctcggcc
     4621 tgatccagaa ggcgggcgcc gatgcgggcc agggctacag cttcatcggc ggcggcgagc
     4681 tcctgtcctc ggcctcccgc gaggcggatg ccggcaccgc cgacctgcgg cgcaacggct
     4741 attacgtgag catcgcgcgg cgcgcggcgc aggcgcgcac gcccgctgcc ggcaaggcgc
     4801 tcagcgtgct gatcgtcgag gacgacccgg tgctggcgaa gatgctcggc cacctgatga
     4861 cgctggaggg cttcgcgccg caggtggcgt tccgcagcgg cgagatcgcg gcggcgttcg
     4921 ggcgcgagcc gccgcccgac ctggtgctgc tcgacgtggt gctgccggac gaggacggct
     4981 tcgcggtgct cgcgcgcatg aaaggcgacg cgcggctgcg cgccatcccg gtgatcatgg
     5041 tcaccggccg caccacgcgc gaaggggtga tgcgcggcct cgccgggggc gccgacggct
     5101 acgtcaccaa gccgatccag tacgaggcgc tggccaacgg ggtgaaggcg gtgctggggc
     5161 tgggcgtcgc cggcgagccg ccggccgcct cggccgagca gctcggcgcc gacgatacct
     5221 tgagcgaatt gcggcgggaa taccgccgcg gcctgccgct caagctggcg cgcatcgagg
     5281 agctggcgaa agccggcaag ctcgaagagc tgctgcggat ggcgcacagc atggcgggcg
     5341 ccgcgcccac gctcggcctg gctccggtcg gcgcggccgc gaaggcgctg gaagaggcgc
     5401 tgctggcgaa agacccggag aaggcggccc gcctggtggc ggcgctgcgc gcggcggcga
     5461 aggccgccgc ctgactcaga actggtagcg gcgcaggccg ccgtcgaccg ggatcaccgc
     5521 gccggtgatg tagcgcgcgc gcggcgaggc gaggaagcac accaggttgg cgatgtcctc
     5581 gggcttgccg tactcgccca ccgggatctc gtgctcggac tgccactggc ggtactcggg
     5641 cgtgtagttg cgcaggatct gctcggagag gatgcgcccc ggcgggatcg agtttacggt
     5701 gatgccgtgc ttgccgacct cgcgcgacag ccccttcgcc caggcgtgca tcgccgcctt
     5761 ggcggcgaag gcgccgttga tgccttcggg ctcgctcttg ccggtgatgt tgacgatgcg
     5821 gccccacttg cgcgccatca tctggccgag cagggcgtgg gtaagctggc gctggcgcgt
     5881 gaagttgagc gtcagcgcct cctcccactg cgcttcgccg gcgtcgagct tgaaggcgcg
     5941 cgagccgccg gcgttgttga cgaggatgtc gacgccgccc aggcccgcga gcgccgcttc
     6001 ggcgatgcgt tgcggcgcgc cctcggcgag gaagtcggcc tcgatgatca ccagttgcgc
     6061 gccggtttcc ttcgccagag tctcgagcag ttcgcgccgc cgcgccgcca ccgcgaggcg
     6121 aacgccctcg gcggcgagcg ccagcgcgat gccgcggccg atgccggccg aggcgccggt
     6181 gaccagcgcg gtcttgcccg cgaggccgag gtccatcagt gcgcgggatg gaacaggtag
     6241 gcgtccatcg ccagcacgcc gccctcgacg tgatcgacga agcgctcggc gcggtaatcc
     6301 gcgtgcgcgg cgcccagcgc cgcgaacagc ggcaggaaat gctcctcggt cgggtgcgcg
     6361 cgcgcgcccg cggcgctcag gcggcggtag tcggcgagcc gctcgagctc gtgcgcctcg
     6421 atgcgctcgc gcacccaggc ctggaattcg gcgacgtagg gctgggcgct gcccacgccg
     6481 gtgtcgcgca ggctgcgcag ctcgcgcagg ttgtgcgtca ggtgcccgga gccgatgatc
     6541 agcacgccct cgccggcgag cggcgcgaga gcggcgccca gcgcgaggtg atggcgcggc
     6601 ccgagccgcg tctgcaccga cacctgcagc accgggacgt cggcgccggg ataggcgtag
     6661 agcagcggcg accaggcgcc gtggtccaga ccgcgctcag ggtcgatgtc ggcctgcagg
     6721 cccgcggcgc gcagcagcgc ctgcgcgcgc agcgcgagcg cgggggcgcc cggcgagggg
     6781 tagcggatct ggtacagggg ctgcgggaag ccgtggaaat cgtggatcgt ctccgggcgc
     6841 gcgctgccgc ccagcatcgg ctgctcggcc tcccagtgcg ccgaggcgat cagcagggcg
     6901 cgcggccgcg gcaggcgctc gccgagcgcc gcccaggcct tgcccgaggc gcccgcttcg
     6961 agcgcgtgca tcggcgagcc gtgcgacagg aacacggtgg gcagcgctgc gccggtcgcc
     7021 tgcatcgccg ttcagatggt ggaggagggg cgccaggtgt agggcaccgg gcccgtggcg
     7081 cgcaacacgc gaatcatgcg cagcgccgct tcgcgcccgc ggtagtaacc gggcacgacg
     7141 atggtttcgt cgaagcccag cgcgcggtag aagccgcgcg ccgcctcgtt ggcggcgcgc
     7201 agctccaggt gcaggctggc gatgccggcg acgcgcgccg attccaccag ccactcgacc
     7261 agccgccggc cgatgcccag gcgccgctcg gccgggcgca ccgccagcag caccaggtgc
     7321 gcgcgctcct cgccgaactc catcagggcg aagccggcga tgcgcccgcc cgcgcaggcg
     7381 agcggcgcca gcgtgtccgg cgcgcggatc gcctgcagca tgcgggcggc gtcgtacttc
     7441 cagcccagcc cggtttccac caggtcgcgc gacatgagcg cgatcgcctg cgcatcgcgc
     7501 ggcgcggcga gccggatgct gacctcgtgc acgctcatgc gcgaagtcta gtcgcaatcg
     7561 cgccgcgttg tcagccgccg gcgtggcgcg cgcgtatcct gacgctgcgc tgcagtccgt
     7621 cggccgcctc cagcagcttc agccgcaggt cggcgtcgag atccccggcg aggacgcgcc
     7681 gcacttcctc cagcgcgcgc gcgtccgact ggccggcaag aaacgcgccc agccagtgct
     7741 cgacgaagaa gatcttgcgc gagcgcttca gttcgggcag gcgcgccagc gcctgcccga
     7801 ggagcggctg cgtgtaggcg gcgtgctccg gggcgttgaa cggcgcgagc gcctcctcca
     7861 cccagccctc gggcaggccg gcgtcgccgg cgaagcgctc gaacatgcgg cgcttggcct
     7921 cggcatcggg cagggcggcg gaggcggcga aggcatagcg gcgcgcatcg tcgccgcgct
     7981 cggttgccgc cagctgcgcc agggcgcgcg ggccgtccgg gtcgccgcgc agcaccagtc
     8041 gctcggcgag gcgaaatcgg tcgcgcacgc gcagctcgac cccgggcagc gcgagcgagc
     8101 cctcgagcag ctgcttcagg ctggcaagcg ccgcgcccga ccaggcgagc gcggcatagg
     8161 tgcgcagcag caggatgcgc cggctcggcg gcgcctcggc gagcagggcg tcgagcagca
     8221 cgcgttccag gcgcggcgcg aggcgttcgc gctgcgcgtc cgacaggtag cggcggaacg
     8281 ccgtctccac ccgacccagc agaccggcga ggatcacgtc gtcgggctcc tccggcgcca
     8341 gcgccagcgc gaactcgatg aaggcgtcgg gcgcgagcgc ggcgtcgcgc acgctctccc
     8401 acagcgcctc gacgagctgc gcgcgcagca ggccgtcggg cagcgcgcgc ggctcggcga
     8461 gcagcgcgtc gcggctctgc gcgtcgagca ggaagcggcc gtagccgaaa tcgccggcat
     8521 tggcgtacag ccagcgcgcc gtcggatgcg ccgccggccc gaccacgcgc acctggcgcg
     8581 actcgagccg cgtctccgag gtgcggatct gcccgtcggt gcccgccgcc gccaccacca
     8641 gcttctgcgc ccacagcccg ccctcgccaa gcgcatcgcg ctgcgcgagc agcaggcgcc
     8701 cctcgctgcg gcgcaggctg atctcgggca ggccgcgccg cgtcacccag gtcccggccc
     8761 aggcgcgcag gtcgcggcgc gaagcgcgct cgaaggcgcg caccaggtcg ctccagtcgg
     8821 cggcgcccca ggcatggcgc gcgaggaaat cgcgcagggc gcggcggaaa gcgcgctcgc
     8881 cgaggtagaa ctccgcctgg cgcagcagcg ccgggccctt ggcgtagacg atcgcgccgt
     8941 aggcggattt cgccgccgac aggttggcca gcggctgccg cagcggcgtg gtgccggcgg
     9001 tggcgtcggt gcgcgcggcg ccggtcttca gcgcgtggaa cgcgctccag ggctccagct
     9061 cgggcagcag cgcggcggcg gccttggccg ccatgaaatt ggcgaagccc tccttcagcc
     9121 acaggtcgtc gaaccagcgc atggtgacca ggttgccgaa ccactggtgc gaggcttcgt
     9181 ggaacagcag ctgcgcgcgg cgcagcaggt ccgggttgct cggcgcgtag gggaacagca
     9241 ccgcctgctc gctgaggaag gtggcgccgg cgtgctccat gccgccgtag gcgagctcgg
     9301 ggatcagcac caggtcgtac ttggcgaagg ggtagggccg cgcgaactcc ttcgcgaaat
     9361 agccgagcgc ggcgcggttc aggcgcagca cttccggcgc ctgcgcgcgc gcgcgctcca
     9421 gctgcgagcg gcgcacgaag aggcgcaccg gctcgcccgg ctgctcgagc acctcgaacg
     9481 gaccggcggc gaaggcgaag agataggtgg agatcggcgc ggtcgcggcg aaacgcgcgc
     9541 gcccctgcgc ctcctccagc gcgggcgcat tggacaccgc gcgccagccc ggcggcaggg
     9601 cgagctcgag cgtgaagcgc gccttcaggt cgggctggtc gaagcaggga aaggcgctgc
     9661 tggcgtccgc cggaacgaac agcgagtaga ggtagctcga gccgtcctcc tggtcgcggt
     9721 agcgcgtcag cgcgctgccg gccggcgctg cgcgggcggc gaactccagg gcgacccggt
     9781 tctcgccctg gcgcagcgcg gcgcgcggca ccaccaggtg ctcccgcgca tggcgcgcct
     9841 tcgccggctt gccgttcacg cgcagcgcgc gcaccggcgc gccgcgccag tcgagcacca
     9901 ggtcgacgcg gcgcgccagc gtcacgcgca gttcgagccg gccggcgacg gcgtcctgcg
     9961 attcgtcgag gcgcagccgc aggtcgtagc gcgggtcgcg gtagtgccgg gcgcgccagc
    10021 gcgccagttc ctgcgacacg cccggttcca ggcgcggcgg ggcctgcgcc tgcgccggcg
    10081 cgccgagcgc cgctatgagg aggaagagcg cggcgcgggc gcgcatgccg cgccggcgct
    10141 cagcgatcca ggcgcgcgaa gccgatggga gcgagcacca gcaggatcac cagcggcacg
    10201 gcgaccccgg gcgccctcgt cgccagcagc agcagcggcc cgatcacgaa cagcgcggcg
    10261 agcagccagg gcacgcgcgg ggccttgccc aggtcggcgc cgagtttctt ccgcagccag
    10321 cgcgccgcgg gtccctgcgc cggtccggtg cggtgcccca gcgagcgcgc cagcgcgcgt
    10381 tcctccagcc aggaaaagag caccagcacc agtacgaaca ggctgccgac caggaccacg
    10441 ggcgcgagca gcaggcccgc cagggccgcg gccggctgcg tgcccaccag cagcagcacc
    10501 ggggtcaggc cgcaggcgat cgccgccacg acgaccagca ggaccgcggg gatcatgagc
    10561 tgccagcgct ccgcgacgta gtcccggaac gcgtccacgt cgcggtagcg ccgcgccagt
    10621 cccaaggcgt cgtccagcgg gccgagcagc aactggccga gccgctgctg gggggtttgc
    10681 ggatgcatgg acggcatggc gcgtgctcct tgtcaggcga tgcaagctca gcatacgaaa
    10741 ctgcgaccgg aaaatccggg aattttgccg cagttcgggg gggctcgctt agactggcgg
    10801 cccatgaaac ccgatttcat tgtcatcctg atcgatgact tgcgctacga cgaattcggc
    10861 gccggcggcc atccgtacat gaaaacgccg caggtggacc gcctggcggc cgagggcgcc
    10921 ctgttcgagc gcgccttcca caccacgccg atctgctcgc ccaaccgggc ctcgatcgtc
    10981 accggccagt acgccagccg ccacggcatc atcgacaacg tggcgcgcga cgccatgagc
    11041 caccgcctgc ccaactacca cctggcgctg cagaagctcg gctacgagac cggccacgtc
    11101 ggcaagtggc acatgggcaa cgacggcatg ccgcgcccgg gatacgacca ctgggtgagt
    11161 ttcgacggcc acggcagcct cagcgacccg cggctgaacg tgaacggcgc ctaccgccgg
    11221 cgcgcgggct acatgaccga catcctcaac cgcgcggcgc tcgagttcgt cgagcaggcg
    11281 cgccgcaagc cctacgcgct gtttttcgcg cacaaggcgg tgcacccgga cgcgcaccag
    11341 gcggccgacg gcacgctcga catgagccgc ttcggcggct acctgcccgc cgagcgccac
    11401 cgcggcttgt accgcggtgc ggtgttccca agaaagccca acatgctttc gccgcgccgg
    11461 ctggtgaagc aggcgcccgc ctgggccgag gctttcaccc tgcgcggcac ggcgcgctcg
    11521 cgggcgctgc tggcttcgat ccaggccggc acgcaggagg agatccgcca gcgcgcgcgc
    11581 atgatggccg cggtggacga gggcgtgggc atgctgctcg cgtcgctcgc gcgccgcggc
    11641 aggctcgacg acaccctgat cctgttcctc ggcgacaacg gctacttctt cggcgagcac
    11701 ggcctgggcc cggagcggcg cttcgcctac gaggagggca tccgcgcgcc gttcgtgctg
    11761 cgctatccgc gccgcgtgcg cgccgggtcg cgcccgcgcg acctggtgat ctgccaggac
    11821 atcgcgccca ccctgatcga gctcgccggc ggccggcccg gcgcgcaggt gcaggggcgc
    11881 tcgctgctgc cgctgctctc cggccgccgc cggagctggc gcaaatcctt cctcatcgag
    11941 tactgggccg agcaggccat gccctggctg gtcggcatga cctacaaggc ggtgcgcacc
    12001 ggccggcaca agctgatccg ctgggtgaac cgcggcgccg gcggcgagct ggacgagctc
    12061 tacgacctcg agcgcgatcc cttcgaactg cagaacctgg cgcgccgcgc cagccacgcg
    12121 cgcattcggg catcattgcg gcgcgacctg cgccgcctgg tggcggacgc cgccggactg
    12181 taacgtcaga ccgcaaggga ggccacttgg actcgatcga ccgccccacc atccagcgct
    12241 cggccaccga catcatcgcc gaggtcaagc tgcagtcgcc cccggcggcg gccgagcggc
    12301 tggcgcattt cggcggcgcc gaggtcgccg ccgtgctcac ccgcctgagt gcgggtttcg
    12361 cccaggagat cctcggcgct ctgcctgccg agtcgcgcga gcgcgcgctg gcggccgcgc
    12421 cgctcgaatt cgcgcgccag tggcagcgca acgcgctgta cggcgagggc accatcgggc
    12481 gcatgatgga gccggtgtac gccgcgtttc cgccgcaggc cacggtcggc caggcgatcg
    12541 aggcgctgca cgagctggtg aagtccgcct tcatcaccta catctacgtg tgcgacgacg
    12601 acgagcggct gctcggcatc gtcaccatgc gggacctcct gttcaacgag cacgccaagg
    12661 cgctgcgcga cgtgatgctg acggaggtgt tcgcgctgca cgcgcccgtg gtgctcgagg
    12721 acgcgatgaa gcaggtgctc gaccgccact atcccgtgta cccggtggtg gacgcggaga
    12781 accgcctgcg cggcctggtg cgcggccaga gcatgttcga ggcgcgcgcc ttcgacatcg
    12841 cggcgcagcc cggcagcatg gtgggcgtcg acaaggagga gcgcctgggc acgccctggc
    12901 agcgcagctt cttcatgcgc cacccctggc tgcagctgaa cctcctgacc gccttcctcg
    12961 ccgcggcggt ggtgggctat ttccaggaca cggtggaccg gctggtgatc ctcgccctgt
    13021 tcctgccggt gctcgccggg cagtccggca acaccggctg ccaggcgctc gcggtgacgc
    13081 tgcgcggcat gaccatcggc gagctcaagc ccggctcgca gaaggcgctg atcacgaagg
    13141 aagccggcct cgggctgctc aacggcgccg gcgtgggcgt cgccgcggcg atcggcatgt
    13201 acgtcaccgc ctccagccag tcgtcgccga acgcgctcat gctgtcgttc gtcgtgttcc
    13261 tggcgatgat cggcgcctgc gtcgcgagcg gcctgtcggg cgccctggtg ccgctcaccc
    13321 tcaagcgcct gggcttcgac ccggccaccg cctccagcat cttcctcacc accgccaccg
    13381 acgtggtgag catgggcatg ctgctggggc tggcgaccat cctggtgcgg taagatgtag
    13441 cgagcgctcc ggccacggga ggtcgacatg caagtcaagg agatcctgcg ggtcaagggc
    13501 aaccggctgc tgagcatcga gccctcgggc cgcgcggtgg acgcggtgag gaccatggcg
    13561 gagcagaacc tcggttcgct catcgtcctc gacaaggagc gcatggtggg cgtgctcacc
    13621 ttccgcgaga tcctgcaggc gctcgccgca cgcgccggcg cgctcggcga cctgcgcgtg
    13681 agcgacgtga tggtgcgcga cccggtgacc gccaccccgg acatggaggt caacgagctg
    13741 cgccgcacca tgctcgattc gggcgcgcgc tacctcccgg tggtgcagga cggcaagctg
    13801 gtcggcgtga tctcgttccg cgacgtcgcc aaggcggtgc tcgaagagca ggacttcgag
    13861 aacaagatgc tcaagggata catcaagaat tggcccgcgt aggaagctcc tcctagccgg
    13921 cggcgcggcg ctcgccgcgg ccggcctcgc cgcctggcgc ctgtggccgg cgcagggaat
    13981 ctggaacccc tgccgcgcgc tgctgccgcg gcgtctcgcc gagcacgagc tggtcaaggc
    14041 tgcctgggac gggctcgacc ccgcgctggt gtgggacgcg catgcgcacc tcgtgggcac
    14101 gggcgacgcg ggcagcgggg tggtggtcaa tccgaggatg gagagcctgc tcaacccggc
    14161 ggagtacgcg cggcgcctgt tcttcctcaa cgcgggctgc gcgcacgacg cgccgggcag
    14221 cgtggaccgg gcctacgtcg agcgcatgcg caacctcgtc gcgggcctgc ggccgggggc
    14281 gaagctcctg ctgttcgcct tcgagcgcgc gcacgacgag cgcggcgcgc cgcagccgga
    14341 gcgctccggc ttcttcgtgc ccgacgccta tgcgcgcgac acggcgcgcg cgcacccgga
    14401 ggcgttcgag tgggcggcct cgatccatcc ctaccgcgcc gactgcgtcg cggcgctgga
    14461 gcgcgccagg gccgagggcg cgcgcgcggt gaagtggctg ccggcggcga tgggcatgga
    14521 ccccggctcg ccgctgtgcg accgcttcta cgccgcgctg gcgcgcttgc gcctgccgct
    14581 gatcacgcac gccgggctgg agcgcgccgt gctgggcacc gacctgcagg actacggcaa
    14641 tccgctgcgc ctgcgccggg cgctggacgc cggcgtgcgc gtcgtggtgg cgcactgcgc
    14701 ctcgatgggc gaggaccgcg acctcgaccg cgggccgcac gggccggcga cggacagctt
    14761 cgcgctgttc tcccgcctga tggacgagcc gcggcacgcg aggcacctct tcggcgacat
    14821 ttccgcggtg acgcaggtga accgcgccgg ccccacgctc gcgcgcctgc tcgagcgcgc
    14881 ggactggcac ccgcggctgc tcaacggctc ggactacccg ctgcccgggc tgatgccgat
    14941 cttctcggtg gattacatcg tctcgctcgg gctgctcgag ccggcggcgg cgccggtgct
    15001 cggcgaaatc cgcgcgcaca acccgctgct gttcgatttc gtgctcaagc gcagcctgcg
    15061 gctgcagggc agggcgctcg cgagctcggt gttcgagacg cggcgcttct tcgagggcgg
    15121 cgtgtagaat cttcggcatg tccggcaaca ctttcggcac gctgtattgc gtcacctcgt
    15181 tcggcgagtc gcacggcccg gcctacggcg gcgtggtcga cggctgcccg ccgggcctgg
    15241 cgctcgaggc ggcggacctg cagcgggaac tggaccggcg caagcccggc agctcgcgcc
    15301 acgtcacgca gcggcgcgag accgacaccg tggagatcct ctccggcgtc ttcgagggga
    15361 aaaccaccgg cacgccgatc ggcttcctgg tgcgcaacga ggaccagcgg cccaaggact
    15421 acgccgagat cgcgcgcaag ttccgcccgg ggcacgccga ctacagctac tggcagaagt
    15481 acggcctgcg cgacccgcgc ggcggcgggc gcgcctcggc gcgcgagacc gtggtgcgcg
    15541 tggcggcggg cgccatcgcc aggaagtggc tcgccgcgcg ctacggcgtg caggtgcgcg
    15601 gctacctcgc ccagctcggg ccgaagaaga tcccgttcca ggactgggcg gcggtggccg
    15661 ccaacccgtt cttcgcgccg aacgccggca tggtggccga gctggagaaa ttcatggacg
    15721 gcctgcgcga ggcgggcgac tcctgcggcg cgcgcgtcaa cgtcgtcgcg caccgggtgc
    15781 cggtgggctg gggcgaaccg gtgtacgggc ggctcgacgc cgacatcgcc gcggcgatga
    15841 tgtcgatcaa cgcggtgaag ggcgtggaga tcggcgccgg cttcgcggca gtggagcaaa
    15901 agggcagcga gcatggcgac gagatgacgc cgcagggatt cgccggcaac aacaacggcg
    15961 gcatcctcgg cggcatctcc agcggccagg acgtgaccgt gtcgatcgcg ctcaagccca
    16021 cttccagcat ccgcctgccg cgccgcacga tcgacctcga aggcaaggcg acgagcgtgc
    16081 agaccaccgg acggcacgat ccctgcgtcg gcatccgcgc cacgccgatc gccgaggcga
    16141 tgctcgcctg cgtgctgatg gaccacgcgc tgcgccaccg cgcgcagtgc ggcgacgtca
    16201 cgccgccgac ccggccggtg tagaaaagcc cgggcgaatc cattagagtg tcaacccacc
    16261 ccacgcaagt gaggcaataa catgagcaac aaggggcagc tgctacaaga cccgttcctg
    16321 aacaccctgc gcaaggaaca cgttcccgtc tcgatctacc tggtgaacgg catcaagctg
    16381 caggggcaga tcgaatcctt cgaccagtac gtggtgctgc tgcgcaactc ggtcacgcag
    16441 atggtgtaca agcacgccat ctccacggtg gtgcccgcgc gcgccgtcag cctcggcgtc
    16501 gaacccgaca aggacgcttg atccccgcgg cggcacccga caaccaggcg gcggtcctcg
    16561 tcagcctgga catcggcact gcggcccccg aggacgccgc cgaagagctg ctgcggctcg
    16621 ccgagagcgc cggcatccgg cgccacgccg cggtgcgcgg ccggcgccag cggcccgacc
    16681 cgaagttcta cgccggcagc ggcaaggtgg cccagatcgg cgcggcggtg gccgcgctca
    16741 atgccggctg ggcgatcttc aaccacgagc tgtcgccggc gcaggagcgc aacctcgagg
    16801 ccgagctgaa ttgccgcgtg ctcgatcgca ccgggctgat cctggagatc ttctcgcagc
    16861 gcgcgcagac cagcgagggc aagctgcagg tggagctggc gcagctcgag cgggcgtcga
    16921 cgcgcctggt gcggggctgg acccacctcg agcgccagcg cggcggcctg ggcaagaccg
    16981 gcggtcccgg cgagaagcag atcgaactgg accggcggta catcgcgcgc cgcgtgcgcg
    17041 tgttgcgcga caggctcgac gtgctgcgcc ggcagcgccg ggtgcagcgc cgctcgcgcg
    17101 tgcggcgcga agtgctgtcg gtgtcgctgg tgggctatac caacgccggc aagtcgacgc
    17161 tgttcaacgc gctcgcccac gccggcgcct acgcagcgga ccagctgttc gccacgctgg
    17221 acaccaccac gcgccgcgtc tggctgcccg aggcgggcaa cgtcgcggtt tccgacaccg
    17281 tcggcttcat ccgcgacctg ccgcacggcc tggtgtcggc gttccgcgcc acgctcgagg
    17341 agacggtgca cgcggacctg cttctgcacg tggtggacgc ggcttctccg gtgcgcgag
//
LOCUS       DASRIE010000201        22880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3865094, whole genome shotgun sequence.
ACCESSION   DASRIE010000201 DASRIE010000000
VERSION     DASRIE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865094"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VFB53_08510"
     CDS             <1..150
                     /locus_tag="VFB53_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HZN86811.1"
                     /translation="AALVCGYAFAWIGHYFFEKNRPATFTYPLWSFMGDWVMWQQILT
                     GKIRF"
     gene            155..1666
                     /locus_tag="VFB53_08515"
     CDS             155..1666
                     /locus_tag="VFB53_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN86812.1"
                     /translation="MLTRLWSSELPYVIVIALVLTALLLRARPGERSTYQNTLWLFLF
                     GMLGVAAAHGALLLHFDTAAGVLQIAFRIVWTIALIRIAGFALFRLLLPKLGKPLPRI
                     IEDLAIIIVYVVYALTQLRVLGVDLTGILATSAIITAVLAFAMQDTLGNVLGGLALQL
                     DHSVRVGDWIQMDGLTGRVTDIRWRSTLVETRTWETVVIPNSALMKGRFVILGRREGA
                     PLQWRRALDFMVDPAVPPARVINLVEEEMREILIANVARAPAPSCVLLRFEHGNLAYR
                     LRYFLTDLQEDEPTDSAVRVHLFASLQRAGLRVAEEQRTVHAVAKDEAHAETVRQREL
                     SRRLSMIKQIDLFAPLSDEERNFVASRLQYAPFARGDIITKQGSVAHWLYIIAFGEAE
                     VRYEQPGRAPRVLGTVRAGQFFGEMGLLAGESRNATVMARSDVECYRLDRASFQGLLL
                     ARPEIADQVSRVIANRKPDLESARAEAADAMAHPAPASEPALLERIQRFFGLK"
     gene            complement(1835..3616)
                     /locus_tag="VFB53_08520"
     CDS             complement(1835..3616)
                     /locus_tag="VFB53_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN86813.1"
                     /translation="MSAYAAPLKDMRFVMQELAGLAEVAKLPGYADATPDTVDAILEE
                     ASKFASQVLDPLNYSGDQEGSVWQEGAVRTPKGFKEAYRQYVDGGWAALPFAQEHGGQ
                     GLPKLVATAVEEMLTSANMSFSLCPLLTQGAIHALELAGSAQLQKAFLHNMVAGKWTG
                     TMNLTEPQAGSDLALVRSKAVREGDHYRISGQKIFITYGEHDLAENIVHLVLARTPEA
                     PEGVKGISLFIVPKFLVKPDGTLGERNGARCASIEHKLGIHASPTAVMLYDNAVGYLV
                     GEENKGLSYMFIMMNAARFSVGLEGVAIAERAFQRALAYAKERLQGRDLAGGGTVPII
                     RHPDVRRMLMLMKSQTEAARALAYVVAAALDVAGRHPDAKLRQQNQAFVDLMIPVVKG
                     WSTETGIEVASLGVQVHGGMGFIEETGAAQHLRDARITTIYEGTTGIQANDLVGRKIA
                     REGGATAKAWLEHLKAFDAELAKSGNADLKAMRAALARGALAVGAAVDYIVANAGKDV
                     TAAFAGAVPFLKLMGIVAGGWQMARAALAAEKKLAAGDKAFYAAKIATARFYADQVLV
                     QAPGLCDTVVKGAGAVMAVPEDSMLAA"
     gene            complement(3627..4562)
                     /locus_tag="VFB53_08525"
     CDS             complement(3627..4562)
                     /locus_tag="VFB53_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN86814.1"
                     /translation="MAVLVLAEHDHQTVRKATLNAVAAAQKIGGEVHVLVAGHNAGAA
                     AKAAAQIAGVAKVLHADAAQLGESLAENVAALLVSLAKGYSHLLAPSTSTGKDVMPRA
                     AALLDVQQISDIVAVESPDTFVRPIYAGNALATVKSSDAIKAITVRTTGFDAVAATGG
                     SAPVESVAAPADSGLSAFVSREVQKSERPELTSARIVVSGGRGMGSGENFKLLEPLAD
                     QLNAAMGASRAAVDAGFVPNDWQVGQTGKIVAPELYVAIGISGAIQHLAGMKDSRVIV
                     AINKDEEAPIFQVADYGIVGDLFQVVPQVIEELKK"
     gene            complement(4562..5311)
                     /locus_tag="VFB53_08530"
     CDS             complement(4562..5311)
                     /locus_tag="VFB53_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZN86815.1"
                     /translation="MKVLVSVKRVVDFNVKVRVKADGTGVETANVKMSMNPFDEIAVE
                     EAVRLKEAGVVTEIVVVSAGVQACQETLRTALAIGADRAILVETEAELQPLAVAKLLQ
                     AVCAKESPQLVILGKQAIDDDCNQTGQMLAALLGWPQATFASKVKIADGRAQVTREVD
                     GGLETLSLRLPAIVTTDLRLNEPRYVTLPNIMKAKKKTLETLKPEALGVDVKPRLATL
                     KVVEPAKRKAGIKVPDAKALVDKLRNEAKVI"
     gene            complement(5343..7406)
                     /locus_tag="VFB53_08535"
     CDS             complement(5343..7406)
                     /locus_tag="VFB53_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZN86816.1"
                     /translation="MTLRAALEPRSIAVVGASDNPHKVGGRPILYMKRYGYRGAIYPI
                     NPGRAEVQGLKAYASIGAAPEAPELAVIAVAGEEAVRAVEECAARGVKVAVVMTSGFG
                     EVGAEGKAAQERMAAAARAAGMRLVGPNCQGLANFATGAVANFSTIFHEMEARDGPVA
                     IVSQSGACSQAIYILARQRGLDVRHVHATGNEADVTVADLAAEIVKEEGVRLLLLYME
                     AIQRPGVLAQAAEFARARDLPIVAVKAGRTASGVRAASSHTGALASEDRVIDAFFEKH
                     GIWRAADPQELVAAGQLYLAGGRPQGRRFVALSNSGASCVMAADAAEEFGLPLPQFAP
                     GTQARLKDVLPAFATPSNPLDVTGALLTDSGLIGAALGVLGEEDACDLLLLAIPVAGT
                     GYDVPRFARDAAAFGARHGKSVALAAPQAAVRAEFEKLGVPAFAREREAMQALSQLAG
                     HSALLRARPAAAAGARGAPALPAGAERFLNEFDSLALLRQAGIAVVEHRLCRDEAEAR
                     AAFRALGPKVVAKACSSSVPHKTEQGLVRLGLESEEAVAQVFRDFKPRLPAADGVLVA
                     RQVSGGRELALGARLDPLFGPVVMLGDGGIHLEALKDFRLLLPPFGEADALRALAGLR
                     AAPLLETQRGKPALDKAAVARMAVALGEAMLGWNGAAASVDVNPVMVFETGACAVDAL
                     VERAL"
     gene            complement(7403..8950)
                     /locus_tag="VFB53_08540"
     CDS             complement(7403..8950)
                     /locus_tag="VFB53_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN86817.1"
                     /translation="MSWDKELEELARRKALAAQLGGAEKVARHKGLGKLTVRERIDGL
                     VDEGSFHEIGSIAGKASYDAAGNLAAFTPANLVMGRARIDGRPVAVAGDDFTVRGGAA
                     DAAIRGKLVTVERMALDLRMPLLRLVDGTGGGGSVKSIEIEGRTLLPSIAMWQICVDN
                     LSQVPVVALALGSVAGLGAARVAASHYSVIVKDTAQMFVAGPPVVARVGEKLEKNELG
                     GSEIHTRNGAIDDEAASETEAFAMARRFLSYLPGSVHELPARTAPQDDPKRRDDWLLS
                     AIPREAKKVYKMRPIVEALVDAGSFFEIGRRWGKPVITGLARLDGWPVAVMASDPYHY
                     GGAWTADACDKVIRFVDLAQTFHLPVVHLVDVPGFLIGREAEEAGTIRHGARAMQAIY
                     QCTVPWCAILVRKVYGVAGAAHMNPARYCMRYAWPSGDWGSLPIAGGLEAAYKAELEA
                     AADPKAKLAEIEARLEKLSSPFRSAESYIVEEIIDPRDTRAILTEFANLAAPLRTPGP
                     PSFGYRP"
     gene            complement(8947..9855)
                     /locus_tag="VFB53_08545"
     CDS             complement(8947..9855)
                     /locus_tag="VFB53_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN86818.1"
                     /translation="MRSPPAPIVRGMLWMAATGALFIVLNTIMKRLSHELDPWLVGFL
                     RYLFGAVVMLGPALHLGAGSLWPKAPGLQFARGLFHAGGMMLWFAALPMVTLAELTAI
                     GFSGPLFICLGAVLFLNERMSGARWAAVLIGFAGVLLVVQPWGDGGVAGISTGMLLML
                     ASAPVFAGSFLVAKALTRRDRSEVVVLWQHLWVSVLLAAFALAYWTRPTPAQWGLLVV
                     CGFLGAGGHYCMTRAFRVADISAVQPVKFLELVWAAILGFLVFGTVPEGATVLGGAVI
                     LVSTLLLARHESRAVAPVKESAREAG"
     gene            9909..10130
                     /locus_tag="VFB53_08550"
     CDS             9909..10130
                     /locus_tag="VFB53_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HZN86819.1"
                     /translation="MAQIRVCCEIAGKVVRLEARAGARVAADDTLLLIECMKMEIPVP
                     APAAGLLEALHVAEGDQVEEGRHVATVRT"
     gene            10108..10467
                     /locus_tag="VFB53_08555"
     CDS             10108..10467
                     /locus_tag="VFB53_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZN86820.1"
                     /translation="MSPPSGPEKQYFERLAAGRFELQKCAACARHVFYPRVLCPHCGG
                     GQLDWVAASGRGTVYSTTVVRRKPADGGDYNVCLVDLAEGVRMMSRVSGIPPAEVRIG
                     MAVKARIAEGLVEFTPA"
     gene            10464..11618
                     /locus_tag="VFB53_08560"
     CDS             10464..11618
                     /locus_tag="VFB53_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HZN86821.1"
                     /translation="MRGSVAIAGVGLAGCGEAPGWSEMEIAAAAAQNALADAGLSARD
                     VDGVVIASTNIFMSGLALAEHLGIRPKFTESSMIGGSSFVGHLIPAALALNAGLCDTV
                     LVAYGSNQRTGTGRAEAGRHKMALDPQPFEHPYKPFNPPSSYALAAARHMHQYGTTRE
                     QLAEVAVAARRWAQLNPEAYAREPLSVQEVLGSRMVSDPLTVRDCCLVTDGGGAYLLT
                     RAERAGDFPRPAAYLLGAASAHWHRQISSMPDLTLTAATESGARAYAMAGLGPRDVDL
                     LALYDAFTINTLLFLEDLGFCAKGEGGAFVSGGRIAPGGSLPVNTNGGGLSCVHPGMY
                     GIFLVIEAVRQLRGEAGARQVPGAQVALAHGNGGVLSSQITALFGTRDTL"
     gene            11615..12394
                     /locus_tag="VFB53_08565"
     CDS             11615..12394
                     /locus_tag="VFB53_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN86822.1"
                     /translation="MTDLSQVKIPSLRSKVSAAEWQARVDLAACYRLIAHFGMNDLVY
                     NHATAGVPGEEGHFLINAYGYAYEEVTASSLVKIDFDGKVVLDSGTGYGINQAGFVIH
                     SAIHRGRRDVACVIHTHSPAGMAVSALKCGLLPLTQNAMFFGEIAYHDYEGPAIDLGE
                     QQRLVRDLGSASAMILRNHGLLTAGRTVCEAFVVLHWLERACQAQLLAMACNTELNHP
                     GAEVIRTTNERYAPGKRRKITELEWPALLRMLDRRDPSFRE"
     gene            complement(12384..13619)
                     /gene="phnA"
                     /locus_tag="VFB53_08570"
     CDS             complement(12384..13619)
                     /gene="phnA"
                     /locus_tag="VFB53_08570"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HZN86823.1"
                     /translation="MGPIEVNGRRYAPLSEPLVVVCVDGCEYDYLTEAAGAGAAPFIA
                     RLLASGSAFKGDCVVPSFTNPNNLSIVTGVPPAVHGICGNYFYDRDADAEVMMNDAKY
                     LRAGTVLAAFSQAGAKVVVITAKDKLRKLLGHGMAGGICFSSEKADQCSLAENGVEDV
                     LGLVGMPLPPVYSAELSEFVFAAGVKLMETRRPGLMYLSTTDYIQHKFAPGSEGANRF
                     YAMMDRYLARLDALGAIVAVTADHGMNAKTRADGSPNVIYLQDAIDGWIGRGRARVIL
                     PITDPYVVHHGALGSFATIYVGEPARAEVAARLARTEGVELVLDNAAACARFELPGDR
                     VGDLVVVSGRHVVLGTSAAKHDLSGLDAPLRSHGGISEQEVPLLFNRRVVDAPRRRLR
                     NFDVLDLALNHLEPRGATP"
     gene            13678..14655
                     /locus_tag="VFB53_08575"
     CDS             13678..14655
                     /locus_tag="VFB53_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HZN86824.1"
                     /translation="MADRVLLTDPIDASGEALLRARGAEVVLAPEGSAATVKRLARDA
                     DGVIIRSKLPEDIFEAAPRLRAVTIHGTGTDLVPLASANARGVLVANLPGENAQSVAE
                     YCVMAMLMLARNAAAITAAMRTRPWDEARKLSGAAREIAGLTAGIVGVGEIGKRVASM
                     CRHGFGMTVLGNQRRMDRLPPEAQAATLDELLARSDFVVIACPLTPQTRHLFNAARLA
                     AMKSSAWLINVGRGPVVDEAALIEALRAQRIAGAMLDVYEHYRIEPGHPLFGLDNVVL
                     TPHLAGSTRESRARASVRAADETLRMLAGEKPRNLVNPEAWHARRPENR"
     gene            14630..15781
                     /locus_tag="VFB53_08580"
     CDS             14630..15781
                     /locus_tag="VFB53_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN86825.1"
                     /translation="MPADLKIAELRAYPTSFPVPPEHRVALGIGTAVKRDAVLVKVTT
                     AGGLVGWGESHHGRAHTAVAKLIETTLRQLVLGMDAADVVGVWQRIYDKQLASHGMGA
                     GTCLAMSGIDMALWDIRGKAAGMPLYRLLGGSRKAIPAYAGGVSLGYQPPAQLIDEVR
                     RSVDAGYKAVKLRVGDSPRRDLERIRAVRKACGDELVILTDANIGYSVEDARQAMPGM
                     DELNVAWLEEPFPAHDHRSYRLAKGFGRTPLAAGENHYTRFEFNRVIEDGAITILQPD
                     LSKTGGITEALRIAAMASAGKLPIHPHSSMTGLNHAASIHFLCAIDNGGYFEGDVSKA
                     NRFRDELVANPGAVDKDGMVWPLEKPGIGLEVNEEFLARHPAIEGPGYI"
     gene            complement(15726..17051)
                     /locus_tag="VFB53_08585"
     CDS             complement(15726..17051)
                     /locus_tag="VFB53_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86826.1"
                     /translation="MGSWRRPWPASLLLLAVLAAYANAFGGSFQFDDFKVIVRQPAVH
                     SLVAWWDSMPGIRPLLKLSYALSWTAAGGGTPAFHAVNVALHAANVLLVAAVLEELWR
                     RIGIADAGFAAFAGALLFALHPAHTEAVTYLSGRSSSMMAFFYLASVLAYLRGGPRWL
                     SPVLFLATLATKEAAVTLPLALLLCDALDPRRPLDLRRQWPHWLVLVAGLALMAALPR
                     YREMLETSLALRSFAEQWQLQLGAIARHAGVLILAVPPNIDPQVAPLPAAAGFAVLAA
                     IGVGFALVRAQPWHAFALLWFFLHLAPTNSLFPRLDAVNDRQLYLASIGPLALAGLAL
                     ALVPRWRVAILTLLAVILGAATALRNMDYRSEAALWSATVEQSPRSARAWNNLGYARQ
                     LEGRRDEACAGYDRALRIDPQYLQARINRLELRCSPDPRSPGAAPGTPR"
     gene            complement(17020..17799)
                     /locus_tag="VFB53_08590"
     CDS             complement(17020..17799)
                     /locus_tag="VFB53_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN86827.1"
                     /translation="MRLALAALLALAGAPAQSQNDPFPGAAAAYLVLVDGAPLWERAA
                     DARRQPASLAKLMSALLVVEEGRLDDEVRVSAAAAKARGARLGLRAGERLPAHALLAG
                     MLVGSANDACLALVERGGGAAGFVARMNAKAPELGLRDTRFGDPCGFDADRQHSTARD
                     LARLATVAMRRAQIAALVGRREIEVATADRARVLRVKNTNALIEHFPGALGVKTGQTG
                     RAGHNLIALAERDGRKVLLVMLGARDRWWDAHGILAQALAR"
     gene            complement(17799..18761)
                     /locus_tag="VFB53_08595"
     CDS             complement(17799..18761)
                     /locus_tag="VFB53_08595"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HZN86828.1"
                     /translation="MRLARSLLAACLSLVAASALAQGGPDELWNMSTRMEMAGMPGQT
                     FPSQVCMKKGEAQPDKMSQDKNCKVTDVRVAGNKTSWKVVCTGRDAMSGTGEITRTRD
                     SMDGRIRLQGKSGGETMDMTMVLSGKLAGGCNAGEEKKKQQAQAKAMVAQGDAQMAKF
                     CKESMDKFMTQMFEGEGAACKAQKAEYCARVSQTAQGMRTSAGYRKSAKAADWEQAGK
                     ACKVNMASVRADACKNAVGGRDWPFVADYCPAEAKRIAAEQCAGRGYTAAMASEYKEV
                     CARHAGRDAEAQKPAAAQQKPAAAPTAGDAVKEGANQLRKLFGR"
     gene            complement(18804..19685)
                     /locus_tag="VFB53_08600"
     CDS             complement(18804..19685)
                     /locus_tag="VFB53_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZN86829.1"
                     /translation="MTRRLAIVNPAAGRGRVRREWPRMAARLREAGLAFDSAETRGPG
                     EAVTLAEHAAKAFDVVIAVGGDGTVHEVANGLLRAGGGAALGVLPLGSGDDFVKMLPA
                     RDAVERLARGTVRRFDAGRIQAGATRYFANGMDIGFGAHASRNVRSVPRLFTGLAAYL
                     GALALTLARYPTLEVRLQLDGGEVFRQSTAMTAVMNGRSFGGSFQVCPDARADDGELD
                     LLIADGVGRLAILGLVPRIMRGTHAGDPRLRLARARRVVIESAAPLLVEADGEIAFED
                     ARRLEIEVLPGALRVLA"
     gene            complement(19682..20851)
                     /locus_tag="VFB53_08605"
     CDS             complement(19682..20851)
                     /locus_tag="VFB53_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN86830.1"
                     /translation="MKLSGLRVIDLSRFLPGPLATQLMADQGAEVIKVESVDEGEPTR
                     EIGAKRDGVSVYFANTQRGKQSLALNLKDPQGVEILLRLAEKSDVLIESFRPGVVDRL
                     GIGYAQVAQRSPRIVYASIAAFGQTGPYRDLATHDLGTEAMTGVLSLNRGGDGKPAIP
                     ALANADMLVATMTLAGVLMALLRRQETGKGDYLDMAMADCLLACLPNNMGVAMAERRQ
                     PDPKSERTLGGNALYNIYETRDADFVVLGGQELKFAQNLLGPLGRPDLVELCRLPPGP
                     GQDPVRAFLRETFLTKTKSQWVEWFKGRDIAFAPMKTLPEVLEDPHFKARRMVLQDAR
                     GWDHLGVPIRFAQEAAKPVLEAPAHGQHSGRILRDLGYADAEVARLKARGVIKDA"
     gene            complement(20851..22032)
                     /locus_tag="VFB53_08610"
     CDS             complement(20851..22032)
                     /locus_tag="VFB53_08610"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN86831.1"
                     /translation="MMLPLAGVRILAVEVYGAGPFGTAHLADLGAEVIKIEQRAGGGD
                     VSRAVGPYFLGADDGHFFQSLNRNKKSLTLDLKHARGREVFMKLAATADGLMGNLRGD
                     QPEKLKITYADLAAANPRIVCAHLSAYGRDGSRKGWPGYDYLMQGEAGWLSLTGEPDG
                     PPSRMGLSVVDYSTGTTCALALLAGILEARRSGRGRDLDVSLFDVAMHTLNYPAAWQL
                     NEGLETGRAPRSAHPFIVPSQLYRTRDGWIFLMAQTQGFWELLCDKLGRQDLKKAYPD
                     FAARREHRDRLTEILDGEFSKRATRDWLEMLRGAVPCGPVLDMAEALENPYFLERGGV
                     QVFDHPDRPGFKLVASPFRLGEPLPARPAPKLGEHTEALLRELGYDEAQIGQLKRCGA
                     V"
     gene            complement(22029..>22880)
                     /locus_tag="VFB53_08615"
     CDS             complement(22029..>22880)
                     /locus_tag="VFB53_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN86832.1"
                     /translation="TEEQKQRFLPGMARGDVRGGLALTEPDCGTDLQAIRTRAVKDGK
                     AYVVNGTKTWISNGIKGSCFALLVKTDPNAQPRHKGMSMFLAEKGKGFRVSKKLEKLG
                     YKGIDSAELIFEDYRLPAENLIGGAEGKGFHHAVGGLELGRINVAARGVGVANAALKD
                     SVAYAQLRKTFGKPICEHQAIQLKLGEMATRCEAARLLVESAARAYDAGERCDMEAGM
                     AKYFASEAALENSIEAVRIHGGYGYSTEFNVERYYRDAPLMCIGEGTNEMQRIIIAKQ
                     LIARNPA"
BASE COUNT         3243 a         8369 c         7908 g         3360 t
ORIGIN      
        1 gcggccctgg tctgcggcta cgccttcgcc tggatcgggc actacttctt cgagaagaac
       61 cgccccgcca ccttcaccta cccgctctgg tccttcatgg gcgactgggt gatgtggcag
      121 cagatcctca ccggcaagat ccggttctag ccccgtgctg acgcgcctct ggagctccga
      181 gctcccctac gtcatcgtca tcgcgctggt gctgaccgcg ctcctgctgc gcgcgcgccc
      241 cggggagcgc agcacctacc agaacacgct gtggctgttc ctgttcggca tgctcggcgt
      301 ggccgccgcc cacggcgcgc tgctgctcca cttcgacacc gccgccgggg tcctgcagat
      361 cgcgttccgc atcgtctgga ccatcgccct gatccgcatc gccggcttcg cgctgttccg
      421 cctgctgctg cccaagctcg gcaagccgct gccgcgcatc atcgaggacc tggcgatcat
      481 catcgtctac gtggtgtacg cgctgacgca gctgcgcgtg ctgggcgtgg acctgaccgg
      541 catcctcgcc acctccgcca tcatcaccgc ggtgctggcc ttcgccatgc aggacaccct
      601 gggcaacgtg ctcggcgggc tggcgctgca gctcgaccac tcggtgcgcg tcggcgactg
      661 gatccagatg gacggcctga ccggccgcgt caccgacatc cgctggcgct cgacgctggt
      721 ggagacgcgc acctgggaga cggtggtgat cccgaacagc gcgctgatga agggccgctt
      781 cgtgatcctc ggccggcgcg agggcgcccc gctgcagtgg cggcgcgcgc tcgatttcat
      841 ggtggacccg gcggtgccgc cggcgcgggt gatcaacctg gtggaagagg agatgcgcga
      901 gatcctgatc gccaacgtgg cgcgcgcccc cgccccgagc tgcgtgctgc tgcgcttcga
      961 gcacggcaac ctcgcctacc ggctgcgcta cttcctcacc gacctgcagg aggacgagcc
     1021 caccgattcc gcggtgcgcg tgcacctgtt cgcctcgctg cagcgcgccg gcctgcgcgt
     1081 cgccgaggag cagcgcaccg tgcacgcggt ggccaaggac gaggcgcacg ccgagacggt
     1141 gcgccagcgc gagctgtcgc ggcgcctgtc gatgatcaag cagatcgacc tgttcgcgcc
     1201 gctgtcggac gaggagcgca acttcgtcgc ctcgcgcctg cagtacgcgc cgttcgcgcg
     1261 cggcgacatc atcaccaagc agggctcggt ggcgcactgg ctctacatca tcgccttcgg
     1321 agaggccgag gtgcgctacg agcagccggg gcgcgcgccg cgcgtcctcg gcacggtgcg
     1381 cgcgggccag ttcttcggcg agatgggcct gctggccggc gaatcgcgca acgccacggt
     1441 gatggcgcgc agcgacgtcg agtgctaccg gctggaccgc gcctcgttcc aggggctgct
     1501 gctggcgcgg ccggagatcg ccgaccaggt gagccgcgtc atcgccaacc gcaagcccga
     1561 cctggagagc gcgcgcgccg aggccgccga cgccatggcc catcccgcgc cggcctcgga
     1621 gccggcgctg ctggagagga tccagcggtt cttcggtttg aagtgaaaat cggaggccgg
     1681 cgccactaac gtggcggtgc ctgcgtttgc ccttccatga tctcgtgttt tttggccggg
     1741 tgaatctttc ggccaaaaaa cacgagaccg ttttgaaagg caaccacccg acctcggcga
     1801 tcgccgtgga gaaaatgggg tctgacccct ttttctacgc cgccagcatc gagtcctcgg
     1861 gcaccgccat caccgcgcct gcgcccttca ccaccgtgtc gcacagcccc ggcgcctgca
     1921 ccagtacctg gtcggcgtag aagcgcgcgg tggcgatctt ggctgcgtaa aacgccttgt
     1981 cgcccgcggc cagctttttc tccgcggcga gcgcggcgcg cgccatctgc cagccccccg
     2041 cgacgatgcc catcagtttc aggaacggta ccgcgccggc gaaggcggcg gtcacgtcct
     2101 tgcccgcgtt ggcgacgatg tagtcgaccg ccgcgcccac ggcgagcgcg cccctggcga
     2161 gcgcggcgcg catcgccttc aggtcggcgt tgcccgactt ggcgagctcg gcgtcgaagg
     2221 ccttcaggtg ctccagccag gccttggcgg tcgccccgcc ctcgcgcgcg atcttgcgcc
     2281 cgacgaggtc gttcgcctgg atgccggtgg tgccctcgta gatggtggtg atgcgcgcgt
     2341 cgcgcaggtg ctgcgcggcg ccggtctcct cgatgaagcc catgccgccg tgcacctgca
     2401 cgccgagcga ggccacctcg atgccggtct cggtgctcca gcccttcacc accgggatca
     2461 tcaggtcgac gaaggcctgg ttctgctggc gcagcttcgc gtccggatgc cgccccgcca
     2521 cgtcgagcgc cgccgccacc acgtaggcga gcgcgcgcgc cgcctcggtc tgcgacttca
     2581 tcagcatcag catgcggcgc acgtcggggt gccggatgat cggcaccgtg ccgccgccgg
     2641 cgaggtcgcg cccctgcagg cgctccttcg cataggcgag cgcgcgctgg aaggcgcgct
     2701 cggcgatcgc cacgccttcc agcccgacgc tgaagcgcgc ggcgttcatc atgatgaaca
     2761 tgtacgacag gcccttgttc tcctcgccca ccaggtagcc gacggcgttg tcgtacagca
     2821 tcaccgccgt cgggctggcg tggatgccga gcttgtgctc gatggaggcg cagcgcgcgc
     2881 cgttcctctc gccgagggtg ccgtctggct tcaccaggaa tttcggcacg atgaagaggg
     2941 aaattccctt gacgccctcg ggcgcctcgg gcgtgcgcgc cagcaccagg tgcacgatgt
     3001 tctcggcgag gtcgtgctcg ccgtaggtga tgaagatctt ctgccccgaa atgcggtagt
     3061 ggtcgccctc gcgcaccgcc ttggagcgca ccagcgcgag gtcggagccc gcctgcggct
     3121 cggtgaggtt catggtgccg gtccacttgc cggccaccat gttgtgcagg aaggcctttt
     3181 gcagctgcgc ggagccggcg agctccagcg cgtggatcgc cccttgcgtg agcagggggc
     3241 agagcgaaaa cgacatgttg gccgaggtca gcatttcctc gaccgcggtg gccaccagct
     3301 tgggcagccc ctggccgccg tgctcctgcg cgaaaggcag cgccgcccag ccgccgtcca
     3361 cgtactgccg gtaggcctcc ttgaatccct tgggcgtcct gaccgcccct tcctgccaga
     3421 ccgagccctc ttgatcacca ctataattca acggatcaag tacttgcgaa gcaaacttcg
     3481 acgcttcctc aagaatggca tcgaccgtgt caggcgtggc gtcggcgtac ccgggcagct
     3541 tcgccacctc ggcaaggccg gcgagttcct gcatcacgaa ccgcatgtcc ttcaatggcg
     3601 cggcgtaggc ggacacggcg acctccctat ttcttcagtt cctcgatcac ctgcgggacc
     3661 acctggaaca ggtctcccac gatgccgtag tcggccacct ggaagatcgg cgcttcctcg
     3721 tccttgttga tcgcgacgat gacgcggctg tccttcatgc ccgccaggtg ctggatcgcc
     3781 ccggaaatgc cgatcgccac gtagagctcg ggcgccacga tcttgccggt ctgcccgacc
     3841 tgccagtcgt tcggcacgaa gcccgcgtcc accgcggcgc gcgaggcgcc catggcggcg
     3901 ttcaactggt cggccagcgg ctcgagcagc ttgaagttct cccccgagcc catgccgcgg
     3961 ccgcccgaga ccacgatgcg cgcggaggtg agctccgggc gctcggactt ctgcacttcc
     4021 cggctgacga aggcggacag cccgctgtcg gccggcgcgg cgacgctctc caccggcgcg
     4081 ctgccgccgg tggcggccac cgcgtcgaac ccggtggtgc gcaccgtgat cgccttgatc
     4141 gcatccgagg atttgacggt ggcgagcgcg ttgccggcgt agatcggccg cacgaaggtg
     4201 tcgggcgact ccaccgcgac gatgtccgag atctgctgca cgtcgagcag cgccgcggcg
     4261 cgcggcatca cgtccttgcc cgtcgaggtc gagggcgcca gcaggtgcga atagcccttg
     4321 gcgagcgaca cgagcagcgc ggcgacgttc tcggcgaggg attcgcccag ctgtgcggcg
     4381 tccgcgtgca gcaccttggc gacgcccgcg atctgcgccg ccgccttggc ggccgcgccc
     4441 gcgttgtggc ccgcgaccag cacgtggacc tcgccgccga tcttctgcgc agcggcgacc
     4501 gcgttgaggg tggccttcct gacggtctgg tggtcgtgct cggcgaggac gaggaccgcc
     4561 atcagatcac cttcgcctcg ttgcgcagct tgtcgaccag cgctttcgcg tccggcacct
     4621 tgatgcccgc cttccgcttg gccggctcca ccaccttgag cgtggcgagg cgcggcttca
     4681 cgtccacgcc gagcgcctcg ggcttgagcg tctcgagcgt cttcttcttc gccttcatga
     4741 tgttcggcag cgtgacgtag cgcggctcgt tgagccgcag gtcggtggtg acgatcgccg
     4801 gcagcctgag cgagagggtc tccaggccgc cgtccacctc gcgcgtgacc tgcgccctgc
     4861 cgtccgcgat cttcaccttc gaggcgaaag tcgcctgcgg ccagccgagc agcgcggcga
     4921 gcatctgccc ggtctggttg caatcgtcgt cgatcgcctg cttgccgagg atcacgagct
     4981 gcgggctctc cttcgcgcag accgcttgca gcagcttggc caccgcgagc ggctgcagtt
     5041 cggcctcggt ctcgacgagg atcgcgcggt cagcgccgat ggcgagcgcg gtgcgcagcg
     5101 tctcctggca ggcctgcacg cccgccgaca ccaccacgat ctccgtgacc acgccggctt
     5161 ccttcagccg caccgcctcc tccaccgcga tctcgtcgaa gggattcatc gacatcttga
     5221 cgttggcggt ctccacgccg gtgccgtcgg ccttgacgcg caccttgacg ttgaaatcga
     5281 ccacgcgctt gacgctgacc aggactttca tcggggagcc gggaggtggg aaacgcgttg
     5341 aattataagg cccgctccac gagcgcgtcg acggcgcagg cgcccgtttc aaacaccatc
     5401 accgggttga cgtcgaccga tgccgccgcg ccgttccagc cgagcatcgc ctcgccgagc
     5461 gcgaccgcca tgcgcgccac cgcggccttg tccagcgccg gcttgccgcg ctgggtttcg
     5521 agcaggggcg ccgcgcgcag cccggcgagc gcgcgcagcg cgtcggcctc gccgaacggc
     5581 ggcagcagca gccggaaatc cttcagcgcc tcgagatgga tgccgccgtc gccgagcatc
     5641 accaccgggc cgaacagcgg gtcgaggcgc gcgccgagcg ccagttcccg gccgccggaa
     5701 acctggcgcg ccaccagcac gccgtcggct gccggcaggc gcggcttgaa gtcgcggaag
     5761 acttgcgcca ccgcctcctc gctctccagc ccgaggcgca ccaggccctg ctcggtcttg
     5821 tgcggcaccg agctggagca ggccttcgcc accaccttcg gcccgagcgc gcggaacgcg
     5881 gcgcgcgcct cggcctcgtc gcggcacagg cggtgctcga ccaccgcgat gcccgcctgc
     5941 cgcagcagcg cgaggctgtc gaactcgttg aggaagcgct cggcgccggc gggcagcgcc
     6001 ggagcgcctc gcgcgccggc agcagcagcc ggccgcgcgc gcagcagcgc gctgtggccg
     6061 gcgagctgac tgagcgcctg catcgcctcg cgttcgcgcg cgaatgcggg caccccgagc
     6121 ttctcgaatt cggcgcgcac cgcggcctgc ggggcggcca gcgccacgct cttgccgtgg
     6181 cgcgcgccga acgccgccgc gtcgcgcgcg aaacgcggca cgtcgtagcc cgtgcccgcg
     6241 acggggatgg cgagcagcag caggtcgcag gcgtcctctt cgcccagcac gccgagggcc
     6301 gcgccgatca gcccgctgtc ggtgagcagc gcgccggtga cgtcgagcgg gttggacggc
     6361 gtggcgaagg cgggcaggac atccttcagc cgcgcctggg tgcccggcgc gaactgcggc
     6421 agcggcaggc cgaattcctc cgccgcgtcg gccgccatca cgcagctcgc ccccgagttg
     6481 ctcagcgcga cgaagcgccg gccctgcggc ctgccgccgg cgaggtagag ctggcccgcg
     6541 gcgaccagtt cctgcgggtc cgccgcgcgc cagatgccgt gcttctcgaa aaacgcgtcg
     6601 atgacgcggt cttcgctcgc cagcgcgccg gtatgcgagg acgcggcgcg cacgccgctc
     6661 gcggtgcgcc cggccttcac cgccacgatc ggcaggtcgc gcgcgcgcgc gaactcggcg
     6721 gcctgcgcca gcacccccgg gcgctggatg gcctccatgt agaggagcag cagccgcacg
     6781 ccctcctcct tgacgatctc cgcggcgagg tccgccaccg tgacgtcggc ctcgttgccg
     6841 gtggcgtgca cgtggcgcac gtcgagcccg cgctggcgcg cgaggatgta gatcgcctgc
     6901 gagcaggcgc cgctctggct gacgatggcc accggcccgt cgcgcgcttc catctcatgg
     6961 aagatggtgg agaagttcgc caccgcgccg gtggcgaagt tcgcgaggcc ctggcaattc
     7021 ggcccgacga ggcgcatccc cgccgcgcgc gccgcggccg ccatgcgctc ctgcgccgcc
     7081 ttgccctcgg cgccgacctc gccgaagccc gaggtcatga ccacggcgac cttcacgccc
     7141 ctcgccgcgc attcctccac cgcgcgcacc gcctcctcgc ccgccaccgc gatcaccgcg
     7201 agctcgggcg cttccggcgc cgcgccgatc gaggcgtagg ccttcaggcc ctgcacctcg
     7261 gcgcggcccg ggttgatcgg atagatggcg ccgcggtagc cgtagcgctt catgtagagg
     7321 atcgggcgcc cgcccacctt gtgcgggttg tccgaggcgc cgaccaccgc gatcgagcgc
     7381 ggctcgagcg ccgcgcgcag cgtcatggcc ggtagccgaa gctcggcggg cctggcgttc
     7441 tcaacggtgc ggccaggttg gcgaactccg tcaggatggc ccgcgtgtcg cgcgggtcga
     7501 tgatctcctc gacgatgtag gactccgcgc tgcggaacgg cgagctgagt ttctccagcc
     7561 gcgcctcgat ctccgccagc ttcgccttcg gatccgccgc cgcttcgagc tccgccttgt
     7621 aggccgcctc cagcccgccc gcgatgggga gcgaacccca gtcgcccgag ggccaggcgt
     7681 agcgcatgca gtagcgcgcg gggttcatgt gcgccgcgcc ggccacgccg tagaccttgc
     7741 gcaccaggat cgcgcaccag ggcaccgtgc actggtagat ggcctgcatg gcgcgcgcgc
     7801 cgtggcggat cgtccccgcc tcctcggcct cgcggccgat gaggaagccc ggcacatcga
     7861 ccaggtgcac caccggcagg tggaaggtct gcgccaggtc cacgaagcgg atcaccttgt
     7921 cgcaggcgtc cgcggtccac gccccgccat agtggtacgg gtcgctcgcc atcaccgcga
     7981 ccggccagcc gtccaggcgc gccaggcccg tgatcaccgg cttgccccag cgccggccga
     8041 tctcgaagaa gctgcccgcg tcgacgagcg cctccacgat cgggcgcatc ttgtagacct
     8101 tcttcgcctc gcgcgggatg gcggagagaa gccagtcgtc gcgacgcttg gggtcgtcct
     8161 gcggcgcggt gcgcgcggga agctcgtgca cggaaccggg gaggtaggac aggaacctcc
     8221 tcgccatggc gaaggcttcg gtttccgagg cggcttcgtc gtcgatcgcg ccgttgcgcg
     8281 tgtgaatctc gctgcccccc agttcgtttt tctccagctt ctcgcccacg cgcgccacca
     8341 ccggcgggcc ggcgacgaac atctgcgccg tgtccttgac gatgaccgaa tagtggctgg
     8401 cggcgacgcg cgccgcgccg aggcccgcca ccgagcccag ggccagcgcc accaccggca
     8461 cctgcgacag gttgtccacg cagatctgcc acatcgcgat gctgggcagg agcgtcctgc
     8521 cctcgatctc gatcgatttc accgagccgc cgccgcccgt gccgtccacc aggcgcagga
     8581 gcggcatgcg caggtcgagc gccatgcgct ccacggtgac cagcttgccg cggatcgccg
     8641 cgtcggcggc gccgccgcgc acggtgaaat cgtcgccggc caccgccacc ggccggccgt
     8701 cgatgcgcgc gcggcccatc accaggttgg ccggcgtgaa ggcggcgagg ttgcccgcgg
     8761 cgtcgtagct cgccttgccg gcgatgctgc cgatctcgtg aaagctcccc tcgtccacca
     8821 ggccgtcgat gcgctcgcgc accgtgagct tgcccagccc cttgtggcgc gccaccttct
     8881 cggcgccgcc gagctgcgcc gccagggcct tgcgccgcgc cagctcctcg agctccttgt
     8941 cccagctcat cccgcctccc gggcggattc tttcacaggc gcgacggcgc gggattcgtg
     9001 ccgcgccagg agcagcgtgc tcaccaggat gaccgcgccg ccgagcacgg tcgcgccttc
     9061 gggcaccgtg ccgaacacga ggaaccccag gatcgccgcc cacaccagct cgagaaattt
     9121 caccggctgg accgccgaga tgtccgccac gcggaaggcg cgcgtcatgc agtagtgccc
     9181 gcccgcgccg aggaagccgc acaccaccag caggccccac tgcgcgggcg tgggccgggt
     9241 ccaataggca agcgcgaagg cggcgagcag caccgacacc cacaggtgct gccagagcac
     9301 caccacctcg gagcggtcgc ggcgcgtcag cgccttggcg acgaggaagg aaccggcgaa
     9361 caccggggcg gaagcgagca tcagcagcat cccggtcgag atgcccgcga cgcccccgtc
     9421 cccccagggc tggaccacca gcagcacgcc ggcgaagccg atcagcaccg cggcccagcg
     9481 cgcgccgctc atgcgctcgt tgaggaacag caccgcgccc aggcagatga acagggggcc
     9541 cgagaagccg atcgccgtca gctcggcgag cgtgaccatc ggcagcgcgg cgaaccacag
     9601 catcatgccc ccggcgtgga acagcccgcg cgcgaactgc agcccgggcg ccttcggcca
     9661 caggctgccc gcgcccaggt gcagcgccgg gccgagcatc acgacggcgc caaagaggta
     9721 tctcaggaag cccaccagcc aggggtccag ctcgtgcgac aggcgcttca tgatcgtgtt
     9781 gagcacgatg aacagcgcgc cggtggcggc catccacagc atgccgcgca cgatcggagc
     9841 gggtggggag cgcatttcga cttccggctc gattgttgca caggagttct gcaataatcg
     9901 cccggcccat ggcgcagatc cgcgtgtgct gcgaaattgc cggcaaggtc gtgcgcctcg
     9961 aggcgcgcgc cggcgcgcgc gtcgccgccg acgacacgct gctcctcatc gaatgcatga
    10021 agatggagat cccggtgccc gcgcccgccg cggggctgct cgaagccctg cacgtcgccg
    10081 agggcgacca ggtcgaggag ggccggcatg tcgccaccgt ccggacctga gaagcagtac
    10141 ttcgagcgcc tggccgccgg gcgcttcgag ctgcagaaat gcgccgcctg cgccaggcac
    10201 gtgttctatc cgcgcgtgct ctgcccgcat tgcggcggcg ggcagctcga ctgggtggcg
    10261 gcgagcggcc gcggcaccgt ctattccacc accgtggtgc gcaggaagcc ggccgacggc
    10321 ggcgactaca acgtctgcct ggtggacctc gccgaagggg tgcgcatgat gagccgcgtg
    10381 tccggcatcc cgcccgccga ggtgaggatc ggcatggcgg tgaaggcgcg catcgccgaa
    10441 ggcctggtgg aattcacgcc ggcatgaggg gctcggtcgc gatcgcgggc gtgggcctcg
    10501 ccggctgcgg cgaggcgccg ggctggagcg agatggagat cgcggccgcc gcggcgcaaa
    10561 atgcgcttgc cgacgcaggg ctcagtgcgc gcgacgtgga cggcgtcgtg atcgccagca
    10621 ccaacatctt catgtcgggc ctggcactcg ccgagcacct gggcatccgc ccgaaattca
    10681 ccgaatcgag catgatcggc ggctcctcct tcgtcgggca cctgatcccg gcggcgctgg
    10741 cgctgaacgc cggcctgtgc gacacggtgc tggtcgccta cggctccaac cagcgcaccg
    10801 gcaccggccg cgccgaggcc ggccggcaca agatggcgct cgacccgcag cccttcgagc
    10861 acccgtacaa gcccttcaac ccgccctcct cctacgcgct cgccgccgcg cgccacatgc
    10921 accagtacgg caccacgcgc gagcagctcg ccgaggtcgc ggtggcggcg cgccgctggg
    10981 cgcagctcaa tcccgaggcc tacgcgcgcg agcccctcag cgtgcaggag gtgctcggct
    11041 cgcgcatggt gagcgacccc ctcacggtgc gcgactgctg cctggtgacc gacggcggcg
    11101 gcgcctacct gctgacgcgc gccgagcgcg ccggcgactt ccccaggccc gccgcctacc
    11161 tcctcggcgc cgcctcggcg cactggcacc ggcagatctc ctcgatgccc gacctgacgc
    11221 tgaccgcggc gaccgaatcg ggcgcgcgcg cctacgccat ggcgggcctc gggccgcggg
    11281 acgttgacct gctggcgctc tacgacgcct tcaccatcaa tacgctcctc tttctcgagg
    11341 acctgggctt ctgcgccaag ggcgaaggcg gcgccttcgt ctcgggcggg cgcatcgcgc
    11401 cgggcgggag cctcccggtg aacaccaacg gcggcggcct ctcgtgcgtg cacccgggca
    11461 tgtacgggat tttcctcgtc atcgaagcgg tgcgacaatt gcgcggcgag gccggcgcgc
    11521 gccaggtgcc gggcgcgcag gtggccctcg cgcacggcaa cggcggggtg ctctccagcc
    11581 agatcaccgc gctcttcggc accagggaca cgctatgacc gacctctcgc aagtgaagat
    11641 tccgtcgctg cgcagcaagg tgagcgccgc cgaatggcag gcgcgcgtcg acctcgccgc
    11701 ctgctaccgc ctgatcgcgc acttcggcat gaacgacctg gtctacaacc atgccaccgc
    11761 gggcgtgccg ggcgaggaag gccatttcct catcaatgcc tacggctatg cctacgagga
    11821 ggtcaccgcc tcgagcctgg tcaagatcga cttcgacggc aaggtggtgc tcgactcggg
    11881 caccggctac ggcatcaacc aggcgggttt cgtcatccac agcgcgatcc accgcgggcg
    11941 ccgggacgtc gcctgcgtga tccatacgca ttcgcccgcc ggcatggcgg tgtcggcgct
    12001 gaaatgcggc ctgctgccgc tcacgcagaa cgcgatgttc ttcggcgaga tcgcctacca
    12061 cgactacgag ggaccggcga tcgacctcgg cgagcagcag cgcctggtgc gcgacctcgg
    12121 ctccgcctcg gccatgatcc tgcgcaacca cggcctgctc accgcgggcc gcacggtgtg
    12181 cgaggctttc gtggtgctgc actggctgga gcgcgcctgc caggcgcagc tgctcgccat
    12241 ggcctgcaac accgagctca accaccccgg cgccgaggtg atccgcacca ccaacgagcg
    12301 ctacgcgccc ggcaagcggc gcaagatcac cgagctggag tggccggcgc tgctgcgcat
    12361 gctcgaccgg cgcgacccct cgttcaggga gtagcgccgc gcggttcgag gtggttcagc
    12421 gcgaggtcca ggacgtcgaa attgcgcagc cggcgccggg gcgcatccac cacgcggcgg
    12481 ttgaacagca gcggcacctc ctgctcggag atcccgccat gcgagcgcag cggcgcgtcg
    12541 agtcctgaca ggtcgtgctt cgccgcgctg gtgccgagca ccacgtgcct gccggagacg
    12601 accaccaggt cgccgacgcg gtcgccgggg agttcgaacc gcgcgcacgc ggcggcgttg
    12661 tccagcacca gttccacgcc ttcggtgcgc gccaggcgcg cggcgacctc ggcgcgcgcc
    12721 ggctccccga catagatcgt ggcgaacgag cccagcgcgc cgtggtgcac cacgtagggg
    12781 tcggtgatcg gcaggatgac gcgcgcgcgg ccgcgcccga tccagccgtc gatcgcatcc
    12841 tgcaggtaga tcacgttggg cgagccgtcc gcccgggtct tcgcgttcat gccgtggtcg
    12901 gcggtcaccg ccacgatggc gcccagcgcg tcgaggcgcg cgagatagcg gtccatcatg
    12961 gcgtagaagc ggttggcgcc ctcgctgccc ggggcgaact tgtgctggat gtagtccgtg
    13021 gtggacaggt acatgagccc gggccgccgg gtctccatca gcttcacgcc ggcggcgaag
    13081 acgaactccg agagctcggc gctgtacacc gggggcagcg gcatgcccac cagcccgagc
    13141 acgtcctcga cgccgttctc cgccaggctg cactggtccg ccttctccga ggaaaagcag
    13201 atgccgcccg ccatgccgtg gccgaggagc ttgcgcagct tgtccttggc cgtaatcacc
    13261 accaccttgg cgcccgcctg cgagaaggcg gccagcaccg tgccggcgcg caggtacttg
    13321 gcgtcgttca tcatcacttc ggcgtcggcg tcgcggtcgt agaaatagtt gccgcagatg
    13381 ccgtgcaccg ccggcggcac gccggtgacg atggacaggt tgttgggatt ggtgaacgag
    13441 ggcaccacgc aatcgccctt gaaggccgag cccgaggcga gcaggcgcgc gatgaacggc
    13501 gccgcgcccg caccggcggc ctcggtgagg tagtcgtact cgcagccgtc gacgcagacc
    13561 accaccagcg gctcgctgag gggcgcgtag cgccggccgt tcacttcgat cgggcccatg
    13621 ctttcagtat gccatcgcgc atcctcgccg cggcgcgggc ctgctacatt ccccgccatg
    13681 gccgaccgcg tcctgctcac cgacccgatc gacgcctcgg gcgaggcgct gctgcgcgcg
    13741 cgcggcgccg aggtggtgct cgcccccgag ggcagcgcgg cgacggtgaa gcgcctggcg
    13801 cgcgatgcgg acggcgtgat catccgctcc aagctgccgg aggacatctt cgaggcggcg
    13861 ccgcgcctgc gcgcggtgac catccacggc acgggcaccg acctggtgcc cctcgcctcg
    13921 gccaacgccc gcggcgtgct ggtggccaac ctgccgggcg agaacgcgca atcggtggcc
    13981 gagtactgcg tgatggcgat gctgatgctg gcgcgcaacg ccgccgccat caccgcggcg
    14041 atgcgcaccc ggccctggga cgaggcgcgc aagctgagcg gcgccgcgcg cgagattgcc
    14101 gggctcacgg cgggcatcgt cggcgtgggc gagatcggca agcgggtggc gagcatgtgc
    14161 cgccacggct tcggcatgac ggtgctgggc aaccagcggc gcatggaccg gctgccgccg
    14221 gaggcgcagg ccgcgacgct ggacgagctg ctcgcccgat ccgacttcgt ggtcatcgcc
    14281 tgcccgctca cgccgcagac gcgccacctg ttcaatgccg cgcggctcgc ggcgatgaag
    14341 tcttccgcct ggctgatcaa cgtcgggcgc ggccccgtgg tggacgaggc ggcgctcatc
    14401 gaggcgctgc gcgcgcaacg catcgcgggg gcgatgctcg acgtctacga gcactaccgc
    14461 atcgaaccgg ggcatcccct gttcggtctc gacaacgtcg tcctcacgcc gcacctcgcc
    14521 ggcagcacgc gcgagtcgcg cgcgcgcgcc agcgtgcgcg ccgccgacga gacgctgcgc
    14581 atgctcgcgg gcgaaaagcc gcgcaacctc gtgaaccccg aagcctggca tgcccgccga
    14641 cctgaaaatc gctgagctca gggcctaccc gacctccttc ccggtgccgc cggagcaccg
    14701 cgtggcgctg ggcatcggca ccgccgtcaa gcgcgacgcg gtgctggtga aggtcaccac
    14761 cgcgggaggg ctggtgggct ggggcgaatc gcaccacggc cgcgcgcaca ccgcggtggc
    14821 gaagctgatc gagaccaccc tcaggcagct ggtcctcggc atggacgcgg cggacgtcgt
    14881 cggcgtgtgg cagcggatct acgacaagca gctcgccagc cacggcatgg gcgccgggac
    14941 ctgcctcgcc atgagcggca tcgacatggc gctgtgggac atccgcggca aggcggccgg
    15001 gatgccgctg tacaggctgc tggggggatc gaggaaagcc atcccggcgt acgcgggggg
    15061 cgtctcgctc ggctaccagc cgccggcgca gttgatcgac gaggtccgca ggagcgtgga
    15121 cgcgggctac aaggcggtga agctgcgcgt gggcgactcg cccaggcggg acctggagcg
    15181 catccgcgcg gtgcgcaagg cctgcggcga cgagctcgtc atcctgacgg acgccaacat
    15241 cggctacagc gtggaagacg cgcgccaggc gatgccgggc atggacgagc tgaacgtcgc
    15301 ctggctggag gagcccttcc ccgcgcacga ccaccgcagc taccggctgg cgaagggctt
    15361 cggccgcacg ccgctcgccg ccggcgagaa ccactacacg cgcttcgagt tcaaccgcgt
    15421 gatcgaggac ggcgccatca ccatcctgca gccggacctg tcgaaaacgg gcggcatcac
    15481 cgaggccctg cgcatcgcgg cgatggcctc cgccggcaag ctccccatcc acccgcacag
    15541 ctcgatgacc ggcctcaacc acgccgcctc gatccacttt ctctgcgcca tcgacaacgg
    15601 cggctacttc gagggcgacg tgtcgaaagc gaaccggttc cgcgacgagc tggtggcgaa
    15661 cccgggcgcc gtggacaagg acggcatggt ctggccgctg gagaagcccg gcatcggcct
    15721 ggaggtcaac gaggagttcc tggcgcggca cccggcgatc gagggtccgg gctacatctg
    15781 agctcgaggc ggttgatcct cgcctgcagg tactgcgggt cgatgcgcaa ggcgcggtcg
    15841 tagccggcgc aggcttcgtc gcggcggcct tcgagctggc gggcatagcc caggttgttc
    15901 caggcgcgcg ccgagcgcgg cgattgctcg accgtggcgc tccacagcgc cgcttcggag
    15961 cggtagtcca tgttcctgag cgcggtcgcg gcgccgagaa tgacagccag cagcgtcagg
    16021 atggcgacgc gccagcgcgg caccagcgcc agcgccagcc cggccagcgc cagcggcccg
    16081 atggacgcca ggtacagctg gcggtcgttc accgcgtcga gacgcgggaa cagcgaattg
    16141 gtcggcgcga ggtgcaggaa gaaccacagc agcgcgaacg catgccaggg ctgcgcccgc
    16201 actaacgcga acccgacccc gatggcggcg agcaccgcaa accccgccgc cgccggcagc
    16261 ggcgccacct gcggatcgat gttgggcggc acggcgagga tcagcacgcc cgcgtgccgc
    16321 gcgatggcgc caagctgcag ctgccactgc tcggcgaatg agcgcagcgc gaggctcgtc
    16381 tccagcatct cgcggtagcg cggcagcgcc gccatgagcg cgagccccgc gaccagcacc
    16441 agccagtgcg gccactgccg ccgcaggtcg aggggccgcc tcgggtcgag cgcgtcgcac
    16501 agcaggaggg caagaggcag cgtcaccgcc gcttccttcg tcgccagggt ggcgagaaac
    16561 agcaccggcg agagccagcg cgggccgccg cgcaggtagg cgagcacgct cgcgaggtag
    16621 aagaaggcca tcatcgagct cgaacggccg gagagatagg tcaccgcctc ggtgtgcgcc
    16681 ggatgcagcg cgaagagcag cgcgccggcg aacgcggcga accccgcatc ggcgatcccg
    16741 atcctgcgcc acagttcttc cagcacggcg gcgaccagga gcacgttggc cgcgtgcagc
    16801 gcgacgttga ccgcatggaa cgcgggcgtg ccgccgcccg ccgcggtcca cgacagggca
    16861 taggagagct tgagcagcgg ccggatgccc ggcatcgagt cccaccaggc gacgagcgag
    16921 tgcacggccg gctggcgcac gatgaccttg aagtcgtcga actggaagct gccgccgaag
    16981 gcgttggcgt aggccgccag caccgcgagg agaagaagac tagcgggcca gggcctgcgc
    17041 caggatccca tgggcgtccc accagcggtc gcgcgcgccg agcatcacca gcagcacctt
    17101 gcggccgtcg cgctcggcga gcgcgatcag gttgtggccg gcgcgccccg tctgcccggt
    17161 cttcacgccc agcgcgccgg ggaagtgctc gatcagcgcg ttggtgttct tcacgcgcag
    17221 cacgcgcgcg cgatcggcgg tcgccacctc gatctcgcgc cggcccacca gcgccgcgat
    17281 ctgcgcgcgg cgcatcgcca ccgtggcgag gcgcgcgagg tcgcgcgcgg tgctgtgctg
    17341 gcggtccgcg tcgaagccgc aggggtcgcc gaagcgcgtg tcgcgcagcc cgagctccgg
    17401 cgccttcgcg ttcatgcgcg cgacgaaccc ggcggcgccg ccgccgcgct ccaccagggc
    17461 gaggcaggcg tcgttggccg agcccaccag catgccggcg agcagcgcgt gcgccggcag
    17521 gcgctcgccc gcgcgcaggc cgagccgcgc gccgcgcgcc ttcgccgccg ccgcgctcac
    17581 ccgcacctcg tcgtccagcc gcccctcctc caccaccagc agcgcgctca tcagcttggc
    17641 gaggctcgcc ggctggcggc gtgcgtccgc cgcgcgctcc cacagcggcg cgccgtccac
    17701 cagcaccagg taggcggccg cggcgccggg aaaggggtcg ttctgggact gcgccggggc
    17761 gcccgccagc gcgagcagcg ccgccagcgc caggcgcact agcgtccgaa cagcttgcgc
    17821 agctggtttg cgccttcctt taccgcgtca ccggcggtgg gcgcggcggc gggcttctgc
    17881 tgcgcggcgg cgggcttctg cgcctcggca tcgcggccgg catggcgcgc gcagacctcc
    17941 ttgtactccg aggccatcgc cgcggtgtag ccgcggcccg cgcactgctc ggcggcgatt
    18001 cttttcgcct ccgccgggca ataatcggcg acgaacggcc agtcgcggcc gcccacggcg
    18061 ttcttgcagg cgtcggcgcg caccgacgcc atgttcacct tgcaggcctt gcccgcctgc
    18121 tcccagtcgg cggccttggc cgacttgcgg tagccggccg acgtgcgcat gccctgcgcg
    18181 gtctggctca cgcgcgcgca gtactcggcc ttctgcgcct tgcacgcggc gccctcgcct
    18241 tcgaacatct gcgtcatgaa cttgtccatc gactccttgc agaacttcgc catctgcgcg
    18301 tcgccctgcg ccaccatcgc tttcgcctgc gcctgctgct tctttttctc ttcgccggcg
    18361 ttgcagccgc cggcgagctt gcccgacagc accatggtca tgtccatggt ctcgccgccg
    18421 ctcttgccct gcaggcggat gcgcccgtcc atgctgtcgc gcgtgcgcgt gatttcgccg
    18481 gtgccggaca tcgcgtcgcg gccggtgcac accaccttcc agctggtctt gttgcccgcg
    18541 acgcgcacgt ccgtgacctt gcagttcttg tcctgggaca tcttgtcggg ctgcgcctcg
    18601 ccttttttca tgcacacctg gctggggaag gtctgccccg gcatccccgc catctccatg
    18661 cgcgtggaca tgttccacag ctcgtccggc ccaccctgcg ccagggcgga tgcggcaacg
    18721 aggctgaggc aggcggcgag cagcgaacgg gcgaggcgca tggttttccc tttggtcgtt
    18781 tgcggcgcga gcatagcaga ggctcaggcg agcacgcgca gcgcgccggg caggacctcg
    18841 atttccaggc ggcgcgcgtc ctcgaaggcg atctcgccgt cggcttccac cagcaggggc
    18901 gcggcggact cgatcaccac gcgccgcgcg cgcgcgaggc gcagccgcgg gtcgccggcg
    18961 tgcgtgccgc gcatgatgcg cggcaccagt cccaggatcg ccagccggcc cacgccgtcg
    19021 gcgatgagca ggtccagttc gccgtcgtcc gcccgggcgt ccgggcagac ctggaagctg
    19081 ccgccgaaac tgcggccgtt catcaccgcc gtcatcgcgg tgctctgccg gaacacctcg
    19141 ccgccgtcga gctgcagccg gacctcgagc gtcgggtagc gcgcaagcgt cagcgccagc
    19201 gccccgaggt aggccgccag gccggtgaac aggcgcggca cgctgcgcac gttgcgcgac
    19261 gcatgcgcgc cgaagccgat gtccatgccg ttggcgaagt agcgcgtggc gccggcctgg
    19321 atgcgccccg cgtcgaagcg gcgcacggtg ccgcgggcga gccgctcgac ggcgtcgcgc
    19381 gcgggaagca tcttgacgaa gtcgtcgccg ctgccgagcg gcagcacgcc gagcgccgcg
    19441 ccgccgcccg cgcgcagcag gccgttggcc acctcgtgca ccgtgccgtc gccgccgacg
    19501 gcgatcacca cgtcgaacgc cttcgccgcg tgctccgcca gggtgacggc ttcgcccggc
    19561 ccgcgcgtct cggcgctgtc gaacgccagc ccggcttcgc gcaggcgagc ggccatgcgc
    19621 ggccattcgc gccgcacccg gccgcgcccg gcggccgggt tgacgatggc gaggcgccgg
    19681 gtcaagcgtc cttgatgacg ccgcgcgcct tcaaccgcgc gacctcggca tccgcgtagc
    19741 ccaggtcgcg caggatacgg ccggagtgct gtccatgcgc cggggcctcg agcaccggtt
    19801 ttgcggcttc ctgcgcgaag cggatgggga cgccgaggtg gtcccagccg cgcgcgtcct
    19861 gcagcaccat gcggcgcgcc ttgaagtgcg ggtcctccag cacctcgggc agcgtcttca
    19921 tcggcgcgaa ggcgatgtcg cgccccttga accattccac ccactgcgac ttggtcttcg
    19981 tcaggaaggt ctcgcgcagg aaggcgcgca ccggatcctg cccgggtccc ggcgggagcc
    20041 ggcacagctc gaccaggtcg gggcggccga gcggcccgag gaggttctgc gcgaacttca
    20101 gctcctgccc tccgagcacg acgaagtccg cgtccctggt ctcgtagatg ttgtagagcg
    20161 cgttgccgcc gagggtgcgc tcgctcttcg ggtcgggctg gcgccgctcc gccatggcga
    20221 cgcccatgtt gttcggcagg caggcaagga gacaatctgc catcgccatg tcgaggtagt
    20281 cgcccttgcc ggtttcctgc ctgcgcagga gcgccatcag caccccggcg agcgtcatcg
    20341 tggcgacgag catgtcggcg ttggcgagcg cgggaatagc gggcttgccg tcgccgccgc
    20401 ggttgagcga gagcacgccc gtcatcgcct cggtgccgag gtcgtgcgtc gcgaggtcgc
    20461 ggtacggccc agtctggccg aaggcggcga tcgaggcgta gacgatccgc ggcgagcgct
    20521 gtgcgacctg cgcgtagccg atgccgaggc ggtccacgac gcccgggcgg aacgactcga
    20581 tcagcacgtc cgacttctcg gcgaggcgca ggaggatctc cacgccctgc gggtccttca
    20641 ggttgagcgc caggctctgc ttgccgcgct gggtgttggc gaaatacacg ctcacgccgt
    20701 cgcgcttcgc gccgatttcg cgcgtcggct cgccctcgtc gaccgactcg accttgatga
    20761 cctcggcgcc ctggtcggcc atcagctggg tggccagcgg ccccggcagg aagcgcgaca
    20821 ggtcgatgac gcgcaggccc gagagcttca tcacaccgct ccgcagcgct tcagctggcc
    20881 gatctgcgct tcgtcatagc ccagttcccg cagcagcgcc tcggtatgct cgccgagctt
    20941 gggcgccggc cttgccggca gcggctcgcc gaggcggaac gggctcgcca ccagcttgaa
    21001 gccgggcctg tccgggtgat cgaacacctg cacgccgccg cgctcgagga agtacgggtt
    21061 ctccagcgcc tcggccatgt cgagcaccgg cccgcagggc accgcgccgc ggagcatttc
    21121 cagccagtcc cgggtggccc gtttcgaaaa ctctccgtcg aggatctccg tcagccggtc
    21181 ccggtgctcg cgccgcgcgg cgaaatcggg gtaggccttc ttcaggtcct ggcggccgag
    21241 cttgtcgcac agcagctccc agaagccctg cgtctgcgcc atgaggaaga tccagccgtc
    21301 gcgcgtgcgg tagagctgcg agggcacgat gaacgggtgc gccgagcgcg gcgcgcgccc
    21361 ggtctccagg ccctcgttca actgccaggc cgcggggtag ttgagcgtgt gcatcgccac
    21421 gtcgaagagc gacacgtcga ggtcgcgccc gcgcccgctg cggcgcgctt ccaggatccc
    21481 ggcgaggagc gccagtgcgc aggtggtgcc ggtggagtaa tccaccaccg acaggcccat
    21541 gcgcgacggc ggcccatctg gctcgccggt cagcgagagc caccccgcct cgccctgcat
    21601 caggtagtcg tagcccggcc agcccttgcg cgagccgtcg cgcccgtagg ccgagaggtg
    21661 ggcgcagacg atcctcgggt tggccgcggc gaggtcggca taggtgatct tcagcttctc
    21721 cggctggtcg ccgcgcaggt tgcccatcag gccgtcggcg gtggcggcga gcttcatgaa
    21781 cacttcgcgg ccgcgcgcgt gcttgagatc gagcgtcagc gacttcttgt tgcggttgag
    21841 cgactggaag aagtggccgt cgtccgcgcc gaggaaatac gggccgacgg cgcgcgagac
    21901 gtcgccgccg ccggcgcgct gctcgatctt gatcacttcg gcccccaggt cggcgaggtg
    21961 cgcggtgccg aaggggccgg cgccgtagac ctccaccgcc aggatgcgca cccccgcgag
    22021 gggcagcatc aggccgggtt ccgcgcgatc agctgcttgg cgatgatgat gcgctgcatc
    22081 tcgttggtgc cctcgccgat gcacatcagc ggcgcgtcgc ggtagtagcg ctcgacgttg
    22141 aactcggtgg agtagccgta gccgccgtgg atgcgcaccg cctcgatcga gttctccagc
    22201 gccgcctcgg aggcgaagta cttcgccatg ccggcttcca tgtcgcagcg ctcgcccgcg
    22261 tcgtaggcgc gcgccgcgct ctccaccagc aggcgcgccg cctcgcagcg ggtcgccatc
    22321 tcgcccagct tcagctggat cgcctggtgc tcgcagatcg gcttgccgaa ggtcttcctc
    22381 agctgcgcgt aggccaccga atccttcagc gcggcgttgg ccacgcccac gccgcgcgcg
    22441 gcgacgttga tgcgccccag ctcaaggccg ccgacggcgt ggtggaaccc tttgccctcg
    22501 gcgccgccga tcaggttctc cgccggcagg cggtagtcct cgaagatcag ctccgccgaa
    22561 tcgatgccct tgtagccgag cttctccagc ttcttcgata cgcggaagcc cttgcccttc
    22621 tcggcgagga acatgctcat gcccttgtgg cgcggctggg cgttggggtc ggtcttcacc
    22681 agcagggcga agcaggagcc cttgatgccg ttggagatcc aggtcttggt gccgttcacc
    22741 acgtaggcct tgccgtcttt cacggcgcgc gtgcggatcg cctgcaggtc ggtgccacag
    22801 tccggctcgg tgagcgcgag gccgccccgc acgtcgccgc gcgccatgcc ggggaggaag
    22861 cgctgcttct gctcctcggt
//
LOCUS       DASRIE010000202         6750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2276391, whole genome shotgun sequence.
ACCESSION   DASRIE010000202 DASRIE010000000
VERSION     DASRIE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276391"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFB53_08620"
     CDS             <1..218
                     /locus_tag="VFB53_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86833.1"
                     /translation="MPHMDGFEFVAALKADTTLPQIPVIFLTSSEDGDERGRALGAVG
                     YLTKPVRADRLLNMVAQHVPGGALPIG"
     gene            complement(240..890)
                     /locus_tag="VFB53_08625"
     CDS             complement(240..890)
                     /locus_tag="VFB53_08625"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZN86834.1"
                     /translation="MRALFLLLLLANLALYAWSRYGVPAAADPALQARPVEPEKLKVI
                     APERLPAPAPAAKKPVPVPVATSCLEWGSFTVADAPRAEQALEPLGLGTRLAQRRTEE
                     TAAWWVFMPSQGSRQGALRKAAELKGLGIDEYFIVQEEGEHRWALSLGVFRSEEAAQA
                     RLAALRTAGVRTAQVGRRETVVPKIWLQVKSVDAALETRLKEIARQIDGSELKACP"
     gene            complement(912..1694)
                     /locus_tag="VFB53_08630"
     CDS             complement(912..1694)
                     /locus_tag="VFB53_08630"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZN86835.1"
                     /translation="MILAIDAGNSRVKWGWLDPATEGRGWHDGVPWASIATVSLIEFA
                     ASSDHVNPFSVTHEDPERIIISNVAGEGAHHLLVNWTRIFDVEPLWLRASVELCGVRN
                     LYERPEQLGPDRWAALIAARALHHAGPALVVNAGTATTVDMLSGAGHFLGGAILPGVE
                     LMRFVLHEHTGRLPLDNGEYRDAPRKTSDAIESGCSHAQAGAVERMYRVLRELELNPL
                     CIVAGGAGRALVDQLSMPRRYVENLVLDGLARIALAERTLAK"
     gene            complement(1691..2431)
                     /locus_tag="VFB53_08635"
     CDS             complement(1691..2431)
                     /locus_tag="VFB53_08635"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZN86836.1"
                     /translation="MDRLDAAAITLPGVEVRVVEHCTSTNDLLLKKIAEGPVLVAAEE
                     QTAGRGRRGRRWHSAPGAGITFSFGRRIERPTRELPALSLVAGVAVARALRGLGVQAK
                     LKWPNDLVVDGAKLGGILVESRNAGHAVIGIGLNCRRDAALEAKLGRKLAYLNNSVTR
                     NEVIRRVGLALLEAIEQFERHGLEALRAEWENMDAHAGQKLRVRLADGRTLSGVAAGL
                     GADGSLQLHTRQGMRAVASGRVVSARAA"
     gene            2448..3950
                     /locus_tag="VFB53_08640"
     CDS             2448..3950
                     /locus_tag="VFB53_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZN86837.1"
                     /translation="MARIAVGGFQHETNTFAPSKADYAAFEAGGGWPGAQYGEPMFSA
                     VEGANIPAEGAIQALRALGHALVGTAWAAANPSAHVTADAFEKILNELVQRLKSAMPV
                     DGVYLDLHGAMVAETFDDGEGEILRRVRDAVGPRVPVAASLDLHANVTPAMTRHADAL
                     VAYRTYPHVDMADTGARAARLLDQMLRTGQRMDGEFRQLDYLTGISSQCSFMEPCRTI
                     YRELGELEEKGGCTLSFTPGFPMADFADCGMSVFGFSKDRGQAGVAVEKLRRIVADSE
                     KDFAIELHPVAEAVQRARRRGKPGAPVILADTQDNPGAGGNGDTTGLLTALIQQGAKD
                     AVLGLLIDPASALLAHDAGPGATLAFNLGGKSGIMGDAPFRGEFTVERLGDGKFTCTG
                     PMYKGVRMTLGPMAVLRSRAAPGVRVVLASRKQQAGDQEMFRHVGIEPRRSRIVALKS
                     SVHFRADFQPMAADVLVVKAPGPALADPVEFKWTKLRKGVRLRPLGPVHA"
     gene            3947..4207
                     /locus_tag="VFB53_08645"
     CDS             3947..4207
                     /locus_tag="VFB53_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86838.1"
                     /translation="MTHEKIKVRNTDSGQVLEVVVFSKRAEMIEVVLGEGVHNVKCSL
                     TPTRTGLAYVGKVMGREIVYERSREQVQADIDRLNPNLKKSR"
     gene            4225..4842
                     /locus_tag="VFB53_08650"
     CDS             4225..4842
                     /locus_tag="VFB53_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZN86839.1"
                     /translation="MAGNMLARFALAIVVACAAGAATAQQMYRWTDQSGRVHITDTPP
                     PPGAKSVRPLKPGGGPASPEAEAAPGSFALQRAIKEFPVTLFTSPNCTEACAGARRVL
                     NQRGVPFSEKQVVDDDSVEELKKVSGGDQVPVIRVGTSVYKGFHPGAYDSLLDSAGYP
                     AAGAVPARAQAAPKPPEDYVAPPEPPKAAPVKPETAAPSGPYAPK"
     gene            4883..6103
                     /locus_tag="VFB53_08655"
     CDS             4883..6103
                     /locus_tag="VFB53_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZN86840.1"
                     /translation="MPALGAEIRLWHSMSGVRGVEFERLVARFNASQPSTRLVAVYKG
                     AYDEASVEAVTARGSRDFAARAPHIVQASEFGGAYLLEQKNAVRPLWQVMGSTGARPG
                     VGPALGGADELLDARGRLLALPLGRATPVLYYNRDSFRIAMLDPARPPATWTDMVPML
                     AALVASGSDCALTLAWPESTLLENMAAWHNQAFVGPDERLMFNTRLSVRWVSTLASWH
                     KAGYFTHSREAEARFAAGECAVLAASSASHADLRGRVKFELGVAQFPYYDDFDSAPLN
                     TVGTGAALWVMAGRPEADYAGVARFLAFFARPEVQADWHQKTGLVPLSAAAYELTRQA
                     GFYKAHPAHEIAVRQLIAKGTPNWKSLRLGQFPRLRSIIDQELESVWQGRKAPVEALN
                     AAVERGNAFLANGR"
     gene            complement(6107..6625)
                     /locus_tag="VFB53_08660"
     CDS             complement(6107..6625)
                     /locus_tag="VFB53_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZN86841.1"
                     /translation="MRLFLTSLLFCGNTAMAACPALLDRTIETIFEKPLSLCEYAGKV
                     LLVVNTASQCGYTPQYDALEALYRKYRARGLVVLGFPSNDFGGQEPGSNKEIAAFCVN
                     QYAIDFPMFAKTNLKANAFYSDLAKATGAPPRWNFHKYLIDRSGRQVQSFDTRVAPND
                     PKLVGAIEKLLE"
     gene            complement(6606..>6750)
                     /locus_tag="VFB53_08665"
     CDS             complement(6606..>6750)
                     /locus_tag="VFB53_08665"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192086.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZN86842.1"
                     /translation="FGLARASNTTPTIVMRFEADNAAALERIKKDFRAALQPLKPNAP
                     LPY"
BASE COUNT         1013 a         2359 c         2360 g         1018 t
ORIGIN      
        1 acatgccgca catggacggc ttcgagttcg tcgccgcgct caaggccgac accacgctgc
       61 cgcagatccc ggtgatcttt ctgacctcgt cggaagacgg cgacgagcgc ggccgggcgc
      121 tcggcgcggt cggctacctg accaagccgg tgcgcgccga ccgcctgctg aacatggttg
      181 cgcagcatgt gccgggcggg gcgctgccca tagggtaaga aggcagggta gaaaggccgc
      241 taggggcagg ccttgagctc gctgccgtcg atctggcggg cgatttcctt cagccgcgtc
      301 tcgagcgccg cgtcgacgct cttcacctgc agccagatct tcggcaccac ggtctcgcgc
      361 cgccccacct gcgcggtgcg tacgcccgcg gtgcgcagcg cggcgagccg cgcctgcgcc
      421 gcctcttcgc tgcggaacac gccgagcgac agcgcccagc ggtgctcgcc ttcctcctgc
      481 acgatgaagt actcgtcgat gccgagcccc ttcaattcgg cggcctttct cagcgcgccc
      541 tggcggctgc cctgcgacgg catgaatacc caccaggcgg cggtctcctc ggtgcgccgc
      601 tgcgccaggc gcgtgccgag cccgagcggc tccagcgcct gctcggcgcg cggggcgtcg
      661 gccacggtga agctgcccca ttcgaggcac gaggtcgcga ccgggaccgg aaccggcttc
      721 ttcgccgccg gagccggcgc cggcaggcgc tcgggcgcga tcaccttgag tttttcgggc
      781 tcgaccgggc gcgcctgcag cgccgggtcg gcggcggccg ggacgccgta gcgcgaccag
      841 gcatagagcg ccaggttggc gagcagcagc aggagaaaca gcgccctcat gccgcgctca
      901 ctgcgccgcc ctcatttggc gagcgtcctc tcggcgagcg cgatgcgcgc gagtccgtcg
      961 agcaccaggt tctcgacgta gcgccgcggc atcgaaagct ggtcgaccag cgcgcgcccg
     1021 gcgccgcccg cgacgatgca cagcgggttc agctcgagct cgcgcagcac gcggtacatc
     1081 cgctccaccg cgccggcctg ggcatggctg cagccgcttt cgatcgcgtc gctcgtcttg
     1141 cgcggcgcgt cgcggtactc gccgttgtcg agcggcaggc gcccggtatg ctcgtggagc
     1201 acgaagcgca tcagctcgac ccccggcagg atcgcgccgc cgaggaagtg gccggcgccc
     1261 gaaagcatgt cgaccgtggt ggcggtgccg gcgttcacca ccagcgccgg gccggcgtga
     1321 tgcagcgcgc gcgcggcgat cagcgccgcc cagcggtcgg ggccgagctg ctccgggcgc
     1381 tcgtagagat tgcgcacgcc gcaaagctcg acgctggcgc gcagccacag cggctcgacg
     1441 tcgaagatgc gggtccagtt gaccagcagg tggtgcgcgc cctcgccggc gacattggag
     1501 atgatgatgc gctcggggtc ctcgtgcgtc accgagaacg ggttgacgtg gtcgctcgac
     1561 gccgcgaact cgatcagcga caccgtcgcg atgctcgccc aggggacgcc gtcgtgccag
     1621 cccctgcctt cagtggcggg gtctagccac ccccacttga cgcgcgaatt gccggcgtcg
     1681 atcgcgagaa tcatgccgcc ctcgccgaca ccacgcgtcc gctggccact gctctcatcc
     1741 cctgccgggt atgaagctgc agtgagccgt cggcacccag gccagcggcg acaccgctca
     1801 gcgtgcgccc gtcggcgagc cgcacgcgca gcttctgccc ggcatgcgcg tccatgtttt
     1861 cccattctgc gcgcaacgcc tccagcccgt gccgctcgaa ctgctcgatg gcctcgagga
     1921 gcgcgagtcc gacccgccgg atgacctcgt ttcgtgtaac cgagttattc aggtaagcga
     1981 gcttgcgccc gagcttggcc tcgagagccg catcgcggcg gcagttgagg ccgatcccga
     2041 tcaccgcatg gccggcattg cggctctcga ccaggatgcc gccgagcttg gcgccgtcga
     2101 cgaccaggtc gttgggccat ttgagcttcg cctggacgcc gaggccacgg agggcgcgcg
     2161 ccaccgcaac cccggcgacc agcgacagcg ccggcagctc gcgcgtcggg cgctcgatgc
     2221 gccggccgaa ggagaaggtg atgcccgcgc cgggcgcgct gtgccagcgc cgcccgcggc
     2281 ggccgcggcc ggcggtctgt tcctcggcgg ccaccagcac cgggccttcg gcgatctttt
     2341 tcagcagcag gtcgttggtg gaagtgcagt gctcgactac gcgcacctct actcccggga
     2401 gggtgatcgc ggccgcatcc agcctgtcca tcctgctagt ctagggcatg gcacgcatcg
     2461 cggtcggcgg cttccagcac gagaccaata cgttcgcgcc gtcgaaggcc gactacgcgg
     2521 cgttcgaggc cggcggcggc tggccgggcg cgcagtacgg cgagcccatg ttcagcgcgg
     2581 tggaaggcgc gaacattcct gccgagggcg cgatccaggc gctgcgcgcc ctgggccacg
     2641 cgctcgtcgg caccgcctgg gccgcggcca atccctcggc ccacgttacc gcggacgcat
     2701 tcgagaagat tctgaatgaa ctggtgcaga gactgaaaag cgccatgccg gtcgacggcg
     2761 tgtatctcga cctgcacggc gcgatggtgg ccgagacctt cgacgacggc gaaggcgaga
     2821 tcctgcggcg cgtgcgcgac gccgtcgggc cgcgcgttcc ggtcgccgcg agcctcgacc
     2881 tgcacgccaa cgtcacgccg gcgatgacgc gccacgccga cgcgctggtc gcctaccgca
     2941 cctacccgca cgtcgacatg gccgacaccg gcgcgcgcgc cgcgcggctg ctggaccaga
     3001 tgctgcgcac cgggcagcgc atggacggcg agttccgcca gctcgattac ctgaccggca
     3061 tctcctcgca gtgctcgttc atggagccct gcaggaccat ctaccgggag ctgggcgagc
     3121 tggaggaaaa aggcggctgc acgctgtcct tcacgcccgg attcccgatg gccgatttcg
     3181 cggattgcgg catgtccgtt tttggtttct cgaaggaccg cgggcaggcc ggggttgcgg
     3241 tcgagaagct gcgccgcatc gtcgccgatt ccgaaaagga tttcgcgatc gaactgcatc
     3301 ccgtggccga ggcggtgcag cgcgcgcgcc gccgcggcaa gccgggcgcg ccggtgatcc
     3361 tcgccgacac ccaggacaac ccgggcgccg gcggcaacgg cgataccacc ggcctgctga
     3421 cggcgctgat ccagcagggc gcgaaagacg cggtgctcgg cctgctgatc gatcccgcgt
     3481 cggcgctgct cgcgcacgac gccgggcccg gcgccacgct ggcgttcaac ctcggcggca
     3541 agtcggggat catgggcgac gcgccgttca gaggcgaatt caccgtcgag cgcctcggcg
     3601 acggcaagtt cacctgcacc ggcccgatgt acaagggcgt gcgcatgacg ctcggtccga
     3661 tggccgttct gcgcagccgc gccgccccgg gcgtgcgcgt ggtgctggct tcgcgcaagc
     3721 agcaggccgg cgaccaggaa atgttccgcc acgtgggcat cgagccgcgc cgcagccgca
     3781 tcgtggcgct gaagagctcg gtccacttcc gcgccgactt ccagccgatg gccgccgacg
     3841 tcctggtggt gaaggcgccg ggtcccgcgc tcgccgaccc ggtggagttc aagtggacca
     3901 agctgcgcaa gggcgtgcgg ctgcggccgc tcgggccggt gcacgcatga cccacgagaa
     3961 gatcaaggtg cgcaacaccg acagcgggca ggtgctcgag gtggtggtgt tcagcaagcg
     4021 cgccgagatg atcgaggtgg tgctgggcga gggcgtccac aacgtcaagt gcagcctgac
     4081 cccgacgcgc accggcctcg cctacgtggg gaaagtaatg gggcgcgaga tcgtgtacga
     4141 gcgcagccgc gagcaggtgc aggccgacat cgaccggctc aacccgaacc tgaagaaatc
     4201 ccgataagcg ctcttcggag taagatggct ggcaacatgc ttgcgcgctt tgccctcgca
     4261 atcgtcgtcg cgtgcgccgc cggcgccgcg acggcgcagc agatgtaccg ctggaccgac
     4321 cagtccggcc gcgtgcacat caccgatacg ccgccgccgc ccggcgcgaa aagcgtgcgc
     4381 ccgctcaagc ccggcggcgg ccccgcgtcg ccggaagccg aggccgcgcc cggctcgttc
     4441 gcgctgcagc gcgcgatcaa ggaatttccg gtgacgctct ttacctcgcc gaactgcacc
     4501 gaggcctgcg cgggggcgcg ccgggttctc aaccagcgcg gcgtgccgtt cagcgagaag
     4561 caggtggtcg acgacgacag cgtcgaagag ctgaagaagg tgtccggcgg cgaccaggta
     4621 ccggtgatca gggtcggcac cagcgtgtac aagggattcc atccgggggc gtacgactcg
     4681 ctgctcgatt ccgccggcta tccggccgcc ggcgcggtgc cggcgcgggc gcaagccgcg
     4741 ccgaagccgc ccgaagacta cgtcgcgcca ccggagccgc ccaaggcagc gccggtcaag
     4801 cccgagacgg ccgcgccgtc cgggccctac gccccgaaat aacctgcgcc tcgtttttct
     4861 cgcgctgttt ttcctctgca gcgtgcccgc gctcggcgcc gagatccgcc tgtggcattc
     4921 gatgagcggc gtgcgcgggg tcgagttcga gcgcctggtc gcgcgcttca atgcgtcgca
     4981 gccgtccacc cggctggtcg ccgtctacaa gggcgcgtac gacgaagcgt cggtcgaagc
     5041 ggtcactgcg cgtggcagcc gcgatttcgc ggcgcgcgcg ccgcacatcg tgcaggcgtc
     5101 ggagttcggc ggcgcgtacc tgctcgagca gaaaaacgcg gtgcggccgt tgtggcaggt
     5161 catgggctca actggcgcgc ggcctggcgt cggacccgcc ctcggcggcg cggacgagct
     5221 cctggacgcg cggggccggc tgctcgcgct accgctcggt cgcgccacgc cggtgctcta
     5281 ctacaaccgc gattccttcc gcatcgcgat gctcgatccg gcgcggccac ccgcgacctg
     5341 gaccgacatg gtgccgatgc tcgcggcgct ggtggcgtcg ggcagcgact gcgcgctcac
     5401 gctggcgtgg cccgagtcga cgctgctcga aaacatggcc gcctggcaca accaggcgtt
     5461 cgtcggtccg gacgagcggc tgatgttcaa cacccggctg tcggtgcgct gggtgtcgac
     5521 gctcgcgagc tggcacaagg cgggctactt cacccacagc cgggaagccg aggcgcgttt
     5581 tgccgccggg gaatgcgccg tgctggccgc gtcctcggcg agccatgcgg acctgcgcgg
     5641 ccgcgtgaag ttcgagctcg gcgtggcgca gttcccctac tacgacgact tcgacagcgc
     5701 gccgctcaac accgtgggca ccggcgccgc gctctgggtc atggcgggca ggcccgaggc
     5761 cgactatgcc ggcgtcgcgc gcttcctcgc gttcttcgcc cggcccgagg tgcaggccga
     5821 ttggcaccag aagaccggcc tggtgcccct cagcgccgcg gcttacgagc tcacgcgcca
     5881 ggcgggcttc tacaaggcgc acccggcgca tgagatcgcg gtgcgccagc tcatcgccaa
     5941 gggcaccccg aactggaaga gcctgcgcct cggccagttt ccgcggctgc gcagcatcat
     6001 cgaccaggag ctcgagtcgg tctggcaggg ccgcaaggcg ccggtcgagg cgctgaatgc
     6061 cgcggtcgag cgcggcaacg cattcctcgc caacggccgg taagggttac tccagcagtt
     6121 tctcgatcgc gccgacgagc ttggggtcgt tcggcgcgac gcgggtgtcg aagctctgca
     6181 cctgccggcc gctgcggtcg atcaggtact tgtggaagtt ccagcgcggc ggcgccccgg
     6241 tggccttggc caggtcggaa tagaaagcat tggcttttag atttgttttt gcaaacatgg
     6301 ggaagtcgat ggcgtattgg ttcacgcaga acgccgcgat ctccttgttg gaacccggct
     6361 cctggccgcc gaaatcgttc gacggaaatc ccagcacgac gaggccgcgc gcgcggtact
     6421 tgcggtagag cgcttccagc gcatcgtatt gcggcgtgta gccgcactgg ctcgcggtat
     6481 tcaccaccag caggaccttg ccggcgtact cgcacagcga caggggtttc tcaaaaatcg
     6541 tttctatcgt gcgatcgagc agggcagggc acgcggccat ggccgtattt ccgcaaaaga
     6601 gaagactagt aaggaagagg cgcattcggc ttcagcggct gcagcgcggc gcggaaatcc
     6661 ttcttgatgc gctcgagcgc ggcggcattg tcggcttcga agcgcatcac gatcgtcggc
     6721 gtggtgttgg aggcgcgtgc cagtccgaag
//
LOCUS       DASRIE010000203         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2277025, whole genome shotgun sequence.
ACCESSION   DASRIE010000203 DASRIE010000000
VERSION     DASRIE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277025"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /gene="selB"
                     /locus_tag="VFB53_08670"
     CDS             complement(<1..1712)
                     /gene="selB"
                     /locus_tag="VFB53_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZN86843.1"
                     /translation="MIVATAGHVDHGKTALVRALTGEDTDRLPEEKRRGMTIDLGFAY
                     LGSIAFVDVPGHERFVRNMAAGVAGIDLALLVVAADDGPMPQTREHLEILDRLGAPRL
                     LAVLSKVDRVAPERAAAARAEVEALLAPGRFAGAEVLPLSSVTGAGLEALRVRLAEHA
                     ARPAARPAARRFRLHVDRAFVLDGQGLVVTGTVLSGEAAVGDEVQLLPAGRRARVRSL
                     RANRGEAELAGAGQRCALALQGLSRGDAERGDIVADAGDAPRSKHLDIALERFAGARL
                     RAGMVGVHLGTAMLAARLAPFGAFGRLSFPKEITAWHGDRLLLRDPGTQRLIASGTVL
                     DPAPPERGAARPERVALLEALSGRDAENAFDALLGLGAVNLDWFARAFHLPPAELQRI
                     AAARPLVRFAQHAMLASQWKAQCDALEHAVAAWHAAQPESVGPRPRDLKGALAAAIDW
                     LVVERRLVRAGPYLHLPGHRPVLSAQDEALWERAAPLLEETPEGKPPRVHELAPLLGM
                     EPKAVADFLQRAARAGRVYRVAPNRFLLPAKVDSLVGLAVALDEESAGRGFPAALYRD
                     RSGLG"
     gene            complement(1709..3082)
                     /gene="selA"
                     /locus_tag="VFB53_08675"
     CDS             complement(1709..3082)
                     /gene="selA"
                     /locus_tag="VFB53_08675"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZN86844.1"
                     /translation="MRLAAIPSVDRILQDPRLAPAIARHGRPLVVEELRALLAEYRSA
                     LKAGSALPASPETIVHALLQRLEAGAQPSLRRVFNLTGTVLHTNLGRALLPAAAIEAA
                     AAALGEPSNLEYDLEAGERGERDDHLEALLCRITGAEAATAVNNNAGAVLLALGALAA
                     GREVPVSRGELVEIGGSFRIPEILAAAGCTLREVGTTNRTHLADYENAVGPDTAALLK
                     VHASNYAVVGFTAAPAEAELARLAQARGLPFVVDLGSGSLVELERYGLPAEPTPMRAL
                     AAGADLVTFSGDKLLGGPQAGLIVGRRELVEKLRKHPLKRALRLDKGRVAALEAVLRL
                     YLDPARLRERLPTLRLLTRPEAEIAALAERMRAPMQGWCGERAAVSVQACASQVGSGS
                     LPVDRLPSAALRLSPSGVKLARLARALRALPIPVIGRTHDDALWLDLRCLEDEAAFLA
                     QLEGRGL"
     gene            3122..4234
                     /locus_tag="VFB53_08680"
     CDS             3122..4234
                     /locus_tag="VFB53_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86845.1"
                     /translation="MRLADAPSLAPAVPEGTDLVEWYYAQGYSDGLPVAPPTPAKVTA
                     MLEALGGEPQQVEARVPPRWGDLTREVLAVNMVMAGCKPEYAPVVRAALLALCEPAFN
                     LNGVQATTHMAAPLLVVNGPLARKIGMNAGCNAFGSGNRANATIGRAIRLVLLNVGGG
                     WPGDLDKATLGHPGKYSYAVAENEELGLLAPYHVEKGFKAEDSTVFVMAAEPPHSVTN
                     HVANDPQGILDSICSAMSTYAHNNAVSSGHCAVVLGPEHATTIARHGWKRHDVKSYLW
                     MNSGNTFGEVSFQHRYGKVYNRSLPVWYKREPDSRIPIVPGAENIHLFVIGGEAGRFS
                     AFIPGWGHMSSPVLREIEGGASAPAGARCVDGTCYL"
     gene            4251..4532
                     /locus_tag="VFB53_08685"
     CDS             4251..4532
                     /locus_tag="VFB53_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86846.1"
                     /translation="MITVYDPRGVVGARQQEIAARIRSLDGLRLGILDNTKWNANKLL
                     RGVRDRLAAGAPLAAVNYYRKESFSRVADPALLEKIRGENDVVVTAIGD"
     gene            4554..4805
                     /locus_tag="VFB53_08690"
     CDS             4554..4805
                     /locus_tag="VFB53_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86847.1"
                     /translation="MHDGVSLETLGVPTAVIVTSTFLHEARVQRAALGMAGLVPVVIE
                     HPLSSLTDAEIGERAAAALEQVLQVWQGRLAEPGGAGVV"
     gene            4815..5021
                     /locus_tag="VFB53_08695"
     CDS             4815..5021
                     /locus_tag="VFB53_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HZN86848.1"
                     /translation="MKTVDLKISGMAGAGAAQALIVALSAVPGVAKADVSLMKKRARI
                     TYDPAKTDVEAFRAAILRAGYRAS"
     gene            5054..>5871
                     /locus_tag="VFB53_08700"
     CDS             5054..>5871
                     /locus_tag="VFB53_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN86849.1"
                     /translation="MRLILCLLLALPPAWASAQPKAPPAMPVRAVPAKLAPAILETGA
                     VGTLRADEAVMIRPEIAGRIDQIKFAEGQQVRQGSLLVTLDAGETRAQVQSSRVQAEL
                     EKQRLERADDLFRKGFSSQQSLDEQRSVHARAAAKLREDQVRLAKSEIRAPFPGVAGL
                     RQVSEGAFVAAGTDIARLEKIDQLKLDFRVPETFLSRIKAGQGVRIAVDSHAGQEFRG
                     AIYALEPAIDEQTRTVLVRARVANADLKLRPGMFARVQLQLGVRENAVWVPEQA"
BASE COUNT          849 a         2154 c         2112 g          756 t
ORIGIN      
        1 cccaggcccg agcggtcgcg gtacagcgcc gccggaaatc cccggcccgc cgactcctca
       61 tcgagcgcga ctgccagccc gaccagggaa tccaccttgg cggggagaag gaatcgattg
      121 ggcgccacgc gatagacgcg acccgcgcgc gccgcgcgct gcaggaaatc ggccaccgcc
      181 ttcggctcca tccccaaaag cggcgcgagc tcgtgcacgc gcggcggctt gccttcgggg
      241 gtttcctcca gcagcggcgc cgcgcgctcc cacagcgctt cgtcctgcgc cgagagtacc
      301 ggccggtggc cgggcagatg cagatacggc cctgcgcgca ccaggcgccg ttctaccacc
      361 agccagtcga tcgccgccgc gagcgcgccc ttcagatccc tgggacgcgg acccaccgac
      421 tccggttgcg ccgcgtgcca ggcagcgacg gcgtgctcca gtgcatcgca ctgcgctttc
      481 cattggcttg cgagcatcgc gtgctgcgcg aaacggacca gcggccgcgc cgccgcgatg
      541 cgctgcaact ccgccggcgg cagatggaag gcgcgcgcga accagtcgag attcaccgcg
      601 ccgaggccga gcagggcgtc gaaggcgttt tcggcgtcgc gcccggagag tgcttcgagc
      661 agcgctacgc gttccgggcg cgccgcgccg cgctccggcg gcgccggatc caaaacggtg
      721 cctgaggcaa tgagccgctg cgtgcccggg tcgcgcagca ggaggcggtc gccgtgccag
      781 gccgtgatct cctttggaaa gctgaggcgc ccgaaggcgc cgaagggcgc gagccgcgcc
      841 gccagcatcg cggtgcccag gtgcacgccg accatgccgg cgcgcaggcg cgccccggcg
      901 aagcgctcca atgcgatgtc gaggtgtttc gagcgcggcg catcgcccgc gtcggcgacg
      961 atgtccccgc gctcggcgtc cccgcgcgag aggccctgca gtgcgagcgc gcagcgctgt
     1021 cccgctcccg cgagttccgc ctcgccgcgg tttgcacgca ggctgcgcac gcgcgcacgg
     1081 cgcccggcgg gcagcaactg cacctcgtcg cccaccgccg cctccccgga gagcaccgtg
     1141 ccggtgacca ccagcccctg gccgtcgagg acgaaggcgc ggtccacgtg caggcgaaag
     1201 cgccgcgcgg cggggcgcgc ggcgggacgg gcggcgtgct cggcaagccg cacacgcagc
     1261 gcctccaggc ctgcgcccgt cacggaggaa agcggcaaca cctcggcgcc ggcgaagcgt
     1321 ccgggcgcga gcagcgcctc gacttcggcg cgcgccgcgg cggcgcgctc cggcgccacg
     1381 cggtcgacct tgctcagcac ggcgagcagg cgcggcgcgc cgagccggtc gaggatctcc
     1441 aggtgctcgc gcgtctgcgg catcggcccg tcgtcggccg ccaccaccag cagcgcgagg
     1501 tcgatgccgg cgacgccggc cgccatgttg cgcacgaagc gctcatgtcc cgggacgtcg
     1561 acaaaggcga tcgatcccag gtaggcgaat cccaggtcga tggtcatgcc gcggcgcttt
     1621 tcctccggca gcctgtcggt gtcttcgccc gtgagggcgc gcaccagcgc ggtcttgccg
     1681 tggtccacgt gcccggcggt ggcgacgatc acaggccgcg gccctcgagc tgcgcgagga
     1741 acgccgcctc gtcctcgagg cagcgcaggt cgagccacag cgcatcgtcg tgggtgcgcc
     1801 cgatcacggg gatcggcagc gcgcgcaagg cgcgcgccag gcgcgcgagc ttcactcccg
     1861 agggcgaaag gcgcagcgcc gcgctcggca ggcggtccac cggcagcgag ccgctgccga
     1921 cctggctcgc gcaggcctgc acgctcaccg cggcgcgctc gccgcaccag ccctgcatcg
     1981 gcgcgcgcat gcgctccgcg agcgccgcga tctccgcctc gggccgcgtc agcaagcgca
     2041 gcgtcggcag ccgctcgcgc aggcgcgcgg gatcgaggta caggcgcagc accgcttcga
     2101 gcgcggcgac gcggcccttg tccaggcgca gcgcgcgctt cagcgggtgc ttgcgcagct
     2161 tctccaccag ctcgcgcctg ccgacgataa gccccgcctg cgggccgccg agcagcttgt
     2221 cgccgctgaa ggtgacgagg tcggcgcccg cggcgagcgc gcgcatcggc gtgggctccg
     2281 caggcaggcc gtagcgctcg agctccacca ggctgccgct gccgaggtcg acgacgaagg
     2341 gcaggccgcg cgcctgcgcc agccgcgcga gctcggcctc ggcgggcgca gcggtgaagc
     2401 cgaccacggc gtagttgctg gcgtgcacct tcaggagcgc cgcggtatcc gggccgaccg
     2461 cgttttcgta gtcggcgagg tgcgtgcggt tggtggtccc cacttcgcgc aaggtgcagc
     2521 ccgccgccgc caggatctcg ggaatgcgga acgagccgcc gatctccacc agctcgccgc
     2581 gcgacaccgg cacctcgcgc cccgcggcga gggcgccgag cgcgagcagc accgcgcccg
     2641 cgttgttgtt caccgcggtc gcggcctcgg cgcccgtgat gcggcaaagc agggcttcga
     2701 gatgatcgtc gcgctcgccg cgctcgcccg cctcgaggtc gtactcgagg ttggagggct
     2761 cgccgagcgc cgccgcggcg gcctcgatcg cggccgccgg cagcagcgca cggccgaggt
     2821 tggtgtgcag caccgtgccg gtgaggttga acacgcgccg cagcgaaggc tgcgcgccgg
     2881 cttccagccg ctgcagcagc gcgtgcacga tggtctcggg cgaggcgggc agcgccgagc
     2941 cggctttcag cgcgctgcgg tattcggcga gcagcgcgcg caattcctcc accaccaggg
     3001 ggcgcccatg ccgggcgatc gccggggcga ggcgcgggtc ctgcaggatc cggtcgaccg
     3061 acgggatggc tgcgagccgc atgctgccat cctaccggcg ccggggtatt cttaggaacc
     3121 tatgcggctc gccgacgctc ccagcctcgc ccccgcggtc cccgagggca ccgatctggt
     3181 cgagtggtat tacgcgcagg gctattccga cggcctgccg gtggcgccgc ccacgccggc
     3241 gaaagtgact gcgatgctcg aggcgctggg cggcgagccg cagcaggtcg aggcgcgcgt
     3301 gccgccgcgc tggggcgacc tcacgcgcga agtgctcgcc gtcaacatgg tgatggcggg
     3361 ctgcaagccg gagtacgcgc cggtggtgcg cgcggcgctg ctcgcgctgt gcgagccggc
     3421 cttcaacctg aacggcgtgc aggcgaccac gcacatggcc gcgccgctcc tcgtggtgaa
     3481 cgggccgctc gcgcggaaga tcggcatgaa cgccggctgc aatgccttcg gctctggcaa
     3541 tcgcgccaac gccaccatcg gccgcgccat ccgcctcgtc ctgctcaacg tcggcggcgg
     3601 ctggccgggg gacctcgaca aggccacgct cggacacccg ggcaagtaca gctacgcggt
     3661 cgcggagaac gaggagctgg gcctgctcgc gccctaccac gtcgagaagg gcttcaaggc
     3721 cgaggactcg accgtgttcg tgatggcggc cgagccgccg cacagcgtca ccaaccacgt
     3781 cgccaacgac ccgcagggga tcctcgactc gatctgctcg gcgatgagca cctacgcgca
     3841 caacaacgcc gtctccagcg gccactgcgc cgtggtgctc gggcccgagc acgccaccac
     3901 catcgccagg cacggctgga agcgccacga cgtcaagagc tacctgtgga tgaattcggg
     3961 caacaccttc ggcgaggtgt cgttccagca ccgctacggc aaggtctaca accgcagcct
     4021 gccggtctgg tacaagcgcg agcccgactc gcgcatcccg atcgtgccgg gcgcggagaa
     4081 cattcatctg ttcgtcatcg gcggggaggc cgggcgcttc tccgccttca tcccgggatg
     4141 ggggcacatg tcctcgcccg tgctgcgcga gatcgagggc ggcgcgtccg cgcccgcggg
     4201 cgcgcgctgc gtcgacggca cctgctatct ttgaacgagg aggtccccgc atgattaccg
     4261 tctacgaccc acgcggcgtc gtcggtgccc ggcagcagga gatcgcggcg cgcatccgct
     4321 cgctcgacgg cctgcgcctc ggcatcctcg acaacaccaa gtggaacgcc aacaagctgc
     4381 tgcgcggcgt gcgcgaccgg ctggcggcgg gcgcgccgct cgccgcggtg aactactacc
     4441 gcaaggagag cttctcgcgc gtcgccgacc cggcgctgct cgagaagatc cgcggcgaga
     4501 acgacgtcgt cgtcaccgcc atcggcgatt gaggctcctg cacatcgtgc tgtatgcatg
     4561 atggcgtcag cctcgagacg ctgggcgtgc cgaccgcggt gatcgtcacc agcactttcc
     4621 tgcacgaggc gcgcgtgcag cgcgccgcgc tcggcatggc gggcctcgtg ccggtggtga
     4681 tcgagcatcc cctgagttcg ctcaccgacg cagagatcgg cgagcgcgcc gccgcggcgc
     4741 tggagcaggt gctgcaggtc tggcagggcc gcctcgcgga acccggcggc gctggtgtag
     4801 tctgacggcc tgcgatgaag accgttgacc tgaagatctc cgggatggcg ggcgccggcg
     4861 cggcgcaggc gctgatcgtg gcgctgtccg cggtacccgg ggtggcgaaa gccgacgttt
     4921 ccctgatgaa gaagcgcgcc cgcatcacct acgacccggc aaagaccgac gtcgaggcgt
     4981 tccgcgccgc catcctgcgc gccggctaca gggcaagcta gggcgcgagc gtatagaatc
     5041 aggcgcttac accatgcgcc tgatcctctg cctgttgctg gccttgccgc cggcctgggc
     5101 gagcgcccag ccaaaggcgc ccccggcgat gcccgtgcgc gccgtgccgg ccaagctcgc
     5161 gcccgcgatc ctcgagaccg gcgcggtcgg caccctgcgc gccgacgagg cggtgatgat
     5221 ccggcccgag atcgccgggc gcatcgacca gatcaaattc gccgaaggcc agcaggtgag
     5281 gcagggcagc ctgctcgtca ccctggatgc gggcgagacg cgcgcgcagg tgcaatcctc
     5341 ccgcgtgcag gcggaactgg aaaagcagcg cctggagcgc gccgacgacc tgttcaggaa
     5401 aggcttcagc agccagcagt cgctcgacga gcagcgctcg gtgcacgcgc gcgccgcggc
     5461 caagctgcgc gaggaccagg tgcgcctcgc caagtcggag atccgcgcgc cgttccccgg
     5521 ggtggcgggc ctgcggcagg tgagcgaagg cgctttcgtg gccgccggca ccgacatcgc
     5581 gcgcctggag aagatcgacc agctgaaact cgacttccgc gtgcccgaga ccttcctgtc
     5641 ccgcatcaag gcgggccagg gagtgcgcat cgcggtcgac tcccacgccg ggcaggaatt
     5701 ccgcggcgcc atctacgccc tggagccggc gatcgacgag cagacgcgca cggtgctggt
     5761 gcgcgcgcgc gtcgccaacg ccgacctcaa gctgcgcccg ggcatgttcg cgcgcgtgca
     5821 gctgcagctc ggggtgcgcg agaacgccgt gtgggtgccg gagcaggcca t
//
LOCUS       DASRIE010000204         6464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2823639, whole genome shotgun sequence.
ACCESSION   DASRIE010000204 DASRIE010000000
VERSION     DASRIE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823639"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..235
                     /locus_tag="VFB53_08705"
     tRNA            161..235
                     /locus_tag="VFB53_08705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Val,seq:cac)
     gene            257..2347
                     /locus_tag="VFB53_08710"
     CDS             257..2347
                     /locus_tag="VFB53_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HZN86850.1"
                     /translation="MSERAIEPQAFLATQPATHAQRRLALGVLLASTVVFLAAAPFAP
                     LRLAAVPAFLPIYQTALVVNELITAVLLFGQFGILRSRALLVLACAYLFSALMAVLHG
                     LSFPGLFAPSGALGGGQQTTAWLYFLWHGAFPLMVIAYALMRDAPEHAEGATRAPLLV
                     GAGSAALAACAFALLTTVGHDALPVIMSGDRDVPAKRIVATASWLLCLAALPVLWRRR
                     PLSTLDLWLMVVMCAWLFDIALAAVLNAGRYDLGWYAGRVYGLLAGSFVLLVMLLENS
                     ALYARLADSHGRHGRRLQILHEIDLALVEEKSPEEIAGAAIQPVREVLGVARAVVNLI
                     DLGAGEAQWLAAAGRQRTHVGPGVRFPLVFMGDVAGLQRGEPQLVQTAALPAGRDRDA
                     LLGSDVHHYMVVPMIAGGELLGALSFGGPRAHFPAAQLTAAREIATQLAIVVQQARLY
                     QRVKVQAGDLETRVRERTAELQAVNKELESFAYSVSHDLRAPLRAVDGYSRLLEEEHA
                     ARLDAEGLRLLGVVRRSATRMGRLIDDLLAFSRFSRQALERQELDMAALVRGVVAELS
                     PQYPAVRVELGELPGARGDRALLSQVWANLVGNALKYSAKSEAPRVHISGKANGAEIE
                     YLVADNGAGFDMRYAEKLFTVFQRLHREDEFPGTGVGLAIVQRIVARHGGRVWAEGET
                     NRGARFGFALPRGG"
     gene            complement(2344..3495)
                     /locus_tag="VFB53_08715"
     CDS             complement(2344..3495)
                     /locus_tag="VFB53_08715"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZN86851.1"
                     /translation="MRRWLFLGLAAASAAQAASEAEVRAIAAHGPWPPAFRADPSNRA
                     SGNPAAIELGERLFFERRLSANGEVSCSRCHLPERNWTDGEARAQGLARVDRNAQSVV
                     NARLQRWFGWDGAQDSLWAQSLRPLLDARELGASAAHVAGLLRADRDLACRYREVFGE
                     LPAEDEAVMVGAAKALAAFQETLVSARTPFDEFRDALLKGDAAGMAKYPEPAKRGLGI
                     FLGKGACNTCHTGPAFSNGEFHDTGVPFFIEPGRVDAGRHEGIRKLRASRFNLLGPYN
                     DDPRRSTATGTTHVTLEHRNFGEFRTPSLRNLALTAPYMHNGRLASLRDVVNHYSTVS
                     PDRLHSDGEAILKPLKLSAQESRDLVAFLESLQDGGASYRRRPLAADCR"
     gene            complement(3492..3857)
                     /locus_tag="VFB53_08720"
     CDS             complement(3492..3857)
                     /locus_tag="VFB53_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HZN86852.1"
                     /translation="MRRWLALALLAPAAQACELPGGAAQSIRSPTHSVVYRAAPRVGE
                     HFAVEFAVCPAPEGVQLDAWMPAHRHGMNYAPSVTALGGGRYRAEGLLFHMPGEWEFV
                     FVLRAGGATQRLVRGLRVE"
     gene            complement(3854..>6464)
                     /locus_tag="VFB53_08725"
     CDS             complement(3854..>6464)
                     /locus_tag="VFB53_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN86853.1"
                     /translation="DVLRIATVVNTMVGQLNAFASEVTRVAREVGTDGKLGGQAQVSG
                     VAGTWKDLTDNVNSMAANLTDQVRNIAEVTTAVARGDLSKKIAVEAKGEVAALADTIN
                     AMIETLATFADQVTDVAREVGIEGKLGGQARVPGAAGLWRDLTDNVNQLAANLTTQVR
                     AIAEVATAVTKGDLTRSIMVEAQGEVAALKDNINEMIVNLKDTTLKNTEQDWLKTHLA
                     RFTRMLQGHRDLVTVSRLILSELAPLVEAQQAAFYIAAGHADAAQLELLASYACPDSL
                     PRSIPLGEGLVGQCAYEKKRIVLGSVPGDYLRVGSALGSGAPGSVMILPVLFEGEVKA
                     VIELASFNRFSDIHQSFLDQLTDSIGVVLHTIAANMRTEDLLQQSQTLTAELQAQQQE
                     LQKTNVRLEQQALNLQQSETLLKSKQEELRGANEQLQEKARQLSEQMRQVELKNREVE
                     QAKAALEDKAEQLALSSRYKSEFLANMSHELRTPLNSLLILARLMADNADGNLSAKQV
                     EFANTIYSSGTDLLSLINDILDLAKIESGTVTLNIAAERFANLRDYVERSFSQLARDK
                     RLPFSVALDPALPQAIQTDGKRLQQIIKNLLSNAFKFTSKGKVALRVGLASAGAAGEA
                     PMVAFSVSDTGIGIPGHKQQVIFEAFQQADGTTSREFGGTGLGLSISRELARLLGGEI
                     RVESEPGRGSTFTLTLPLAYGAGAEGADPAVQVQEPALAAPAALEPAPRRPVSRQARS
                     ALKGKRALLVDDDIRNVFALTSALEQYGMVVAHAENGREGIEALKRLQDVDVVLMDLM
                     MPDLDGYDTIRIVRRHKRYRKLPIIAVTAKAMLGDREKCISAGATDYIAKPVDVEDLL
                     HKLSRHWRRPAAK"
BASE COUNT          813 a         2327 c         2290 g         1034 t
ORIGIN      
        1 cgactgctga tcaaaaagct gtctgaaaat cacgtggcga actttacccc agtcgcatcg
       61 ggctgcggcg gctgctgccc cagcccggcg gtcaccgctc tccgggggtt tgcttgctaa
      121 aatcctgcct ccgaccgtta cgcagtaacc cacaacggca gggtcgttag ctcagcggta
      181 gagcactgct ctcacacagc aggggccact ggttcgatcc cagtacgacc caccaacccc
      241 ccgggatcgt ccccgcatga gcgagcgcgc catcgagcca caggccttcc tggcgacgca
      301 gccggccacc cacgcccagc gccgtctcgc gctcggcgtg ctgctcgcct cgacagtggt
      361 tttcctggcc gcggcgccgt tcgcgccgtt gcgcctcgcc gcggtgcccg ctttcctgcc
      421 gatctaccag acggcgctgg tggtcaacga gctgatcacc gcggtgctgc tgttcggcca
      481 gttcggcatc ctgcgctcgc gcgcgctgct ggtgctcgcc tgcgcctacc tgttcagcgc
      541 gctgatggcg gtgctgcacg ggctcagctt cccgggcctg ttcgcgccgt cgggggcgct
      601 gggcggcggg cagcagacca cggcgtggct gtatttcctc tggcacggcg cctttccgct
      661 gatggtgatc gcctacgcgc tgatgcgcga cgcaccggag cacgcggaag gcgccacgcg
      721 cgccccgctg ctggtcggcg cgggcagcgc ggcgctggcg gcgtgcgcct tcgcgctgct
      781 gaccaccgtg gggcacgacg cgctgccggt gatcatgagc ggcgaccgcg acgttcccgc
      841 caagcgcatc gtcgccaccg cctcgtggct gctgtgcctc gccgccctgc cggtcctctg
      901 gcggcggcgg ccgctgtcga cgctcgacct gtggctgatg gtggtgatgt gcgcctggct
      961 gttcgacatc gcgctggcgg cggtgctgaa cgccggccgc tacgacctgg gctggtacgc
     1021 ggggcgcgtc tacggcctgc tggccggctc cttcgtgctg ctggtgatgc tgctggagaa
     1081 cagcgcgctg tacgcgcggc tggccgattc gcacggccgg cacggcaggc ggctgcagat
     1141 cctgcacgag atcgacctcg cgctggtgga ggagaagtcg cccgaggaga tcgcgggcgc
     1201 ggccatccag ccggtgcgcg aggtcctcgg cgtggcgcgc gcggtggtga acctgatcga
     1261 cctcggcgcc ggcgaggcgc agtggctggc cgccgccggc cgccagcgca cccacgtcgg
     1321 tcccggcgtg cgcttcccgc tggtgttcat gggcgacgtc gcgggcctgc agcgcggcga
     1381 gccgcagctg gtgcagaccg cggcgctgcc ggccggcagg gaccgtgacg cgctgctcgg
     1441 gtcggacgtg caccactaca tggtggtgcc gatgatcgcc ggcggcgagc tgctcggggc
     1501 gctcagcttc ggcggcccgc gggcgcattt tcccgccgcg cagctcaccg cggcgcgcga
     1561 gatcgccacg cagctggcga tcgtcgtgca gcaggcgcgc ctgtaccagc gcgtcaaggt
     1621 gcaggcgggc gatctggaga cgcgggtgcg cgagcgcacc gccgagctgc aggcggtgaa
     1681 caaggaactc gagtccttcg cctactcggt gtcgcacgac ctgcgcgccc cgctgcgcgc
     1741 cgtggacggc tattcgcgcc tgctcgagga ggagcacgcc gcgcggctgg acgccgaggg
     1801 cctgcgcctg cttggcgtgg tgcgccgctc ggccacgcgc atggggcgcc tgatcgacga
     1861 cctgctcgcc ttctcgcgct tcagccggca ggcgctcgag cgccaggaac tcgacatggc
     1921 ggcgctggtg cgcggcgtgg tggcggagct ctcgccgcag tatcccgcgg tgcgcgtcga
     1981 actgggcgag ctgcccgggg cgcgcggcga ccgggcgctc ctgtcgcagg tgtgggccaa
     2041 cctggtgggc aacgccctca agtacagcgc gaagagcgaa gcgccgcgcg tccacatcag
     2101 cggcaaggcg aacggcgcgg agatcgagta cctcgtcgcc gacaacggcg ccggcttcga
     2161 catgcgctac gccgagaagc tgttcaccgt gttccagcgc ctgcaccgcg aggacgagtt
     2221 ccccggcacc ggcgtcgggc tggcgatcgt gcagcgcatc gtcgcccggc acggcggccg
     2281 agtgtgggcc gagggcgaga ccaaccgggg ggcgcgcttc ggtttcgcgc tgccgcgcgg
     2341 cggctagcgg cagtccgcgg cgagcgggcg gcgccggtag ctcgccccgc cgtcctgcaa
     2401 ggactcgagg aacgccacca ggtcgcggga ttcctgcgcc gagagcttga gcggcttgag
     2461 gatcgcctcg ccgtccgagt gcaatcggtc cgggctgacg gtcgaatagt ggttcaccac
     2521 gtcgcgcagg ctggccaggc gcccgttgtg catgtacggc gccgtcaggg ccaggttgcg
     2581 cagcgacggc gtcctgaact cgccgaagtt gcggtgctcc agcgtcacgt gcgtcgtgcc
     2641 ggtcgccgtg ctgcgcctgg gatcgtcgtt gtagggcccg agcaggttga atctgctggc
     2701 gcgcagtttc cggatcccct catgccgccc cgcgtccacc ctgcccggct cgatgaagaa
     2761 cgggaccccg gtgtcgtgga attcgccgtt gctgaacgcg ggccccgtgt ggcaggtgtt
     2821 gcaggcgccc ttgccgagga agattcccag gccgcgcttc gccggttcgg ggtatttcgc
     2881 catgccggcc gcatcgccct tcagcagcgc gtcgcggaac tcatcgaacg gcgtgcgagc
     2941 gctgaccagc gtctcctgga acgccgccag cgccttcgcc gcgcccacca tcacggcctc
     3001 gtcctccgcc ggcaactccc cgaacacctc gcgatagcgg caggcgaggt cccggtctgc
     3061 gcgcaggagc ccggcgacgt gggccgcgct cgcgcccagc tcgcgcgcat cgagcagcgg
     3121 ccggaggctt tgcgcccaca ggctgtcctg cgccccgtcc cagccgaacc agcgctgcag
     3181 gcgcgcgttg acgacgctct gcgcgttgcg atcgacgcgc gccaggccct gcgcgcgcgc
     3241 ttcgccgtcg gtccagttcc tctccggcag gtggcagcgc gagcaggaga cctcgccgtt
     3301 cgccgacagg cgccgctcga agaacaggcg ctcgccgagc tcgatcgccg cgggatttcc
     3361 cgaagcgcgg ttcgagggat cggcgcggaa cgccggcggc cagggtccgt gcgccgcgat
     3421 ggcgcggacc tcggcctcgc tggcggcctg cgccgccgag gcggcggcca ggccgaggaa
     3481 aagccagcgc ctcactcgac gcgcaggccg cgcaccagcc gctgcgtggc gccgcccgcg
     3541 cgcagcacga acacgaactc ccattcgccc ggcatgtgga acagcagccc ctccgcgcgg
     3601 tagcgcccgc cgcccagcgc cgtgacgctc ggcgcgtagt tcatgccgtg gcggtgcgcc
     3661 ggcatccagg cgtccagctg cacgccctct ggcgccgggc agaccgcgaa ctccacggcg
     3721 aagtgctcgc ccacgcgcgg cgccgcgcgg tacaccacgc tgtgggtcgg cgagcggatg
     3781 gactgcgccg ccccgccggg gagctcgcag gcctgcgccg cgggcgccag cagcgccagc
     3841 gccagccaac gcctcatttc gccgcgggcc ggcgccagtg gcgcgagagc ttgtgcagca
     3901 ggtcctcgac gtccaccggc ttggcgatgt agtcggtggc gcccgcgctg atgcacttct
     3961 cgcggtcgcc cagcatcgcc ttggcggtga ccgcgatgat cggcagcttg cggtagcgct
     4021 tgtgccgccg cacgatgcgg atggtgtcgt agccgtcgag gtcgggcatc atcaggtcca
     4081 tcaataccac gtcgacgtcc tgcaggcgct tgagcgcctc gatgccctcg cggccgttct
     4141 cggcgtgcgc caccaccatg ccgtactgct ccagcgcgct ggtgagcgcg aacacgttgc
     4201 ggatgtcgtc gtccaccagc agggcgcgct tgcccttgag cgcgctgcgc gcctgcctgg
     4261 acaccgggcg ccgcggcgcg ggctccagcg ccgccggcgc ggcgagcgcc ggctcctgca
     4321 cctgcactgc cgggtccgcg ccctccgccc cggcgccgta ggcgagcggc agggtcagcg
     4381 tgaaggtgct gccgcggccg ggctcgctct cgacgcggat ctccccgccc agcaggcgcg
     4441 ccagctcgcg gctgatcgac aggccgaggc cggtgccgcc gaactcgcgg ctggtggtgc
     4501 cgtcggcctg ctggaaggcc tcgaagatca cctgctgctt gtgccccggg atgccgatgc
     4561 ccgtatcgct caccgagaag gcgaccatcg gcgcctcccc tgcggcgccc gccgaggcga
     4621 gcccgacgcg cagcgccacc ttgcccttgc tggtgaactt gaaggcgttg gacagcaggt
     4681 tcttgatgat ctgctgcagc cgcttgccgt cggtctggat ggcctgcggc agcgccgggt
     4741 cgagcgccac gctgaacggc agccgcttgt cgcgcgccag ctggctgaag gagcgctcga
     4801 cgtagtcgcg caggttggcg aagcgctcgg cggcgatgtt gagcgtcacc gtgcccgact
     4861 cgatcttcgc caggtcgagg atgtcgttga tcagcgaaag caggtcggtg cccgaggagt
     4921 agatggtgtt ggcgaactcg acctgcttgg cgctgaggtt gccgtcggcg ttgtcggcca
     4981 tcaggcgcgc caggatcagc aggctgttca ggggcgtgcg cagctcgtgc gacatgttgg
     5041 cgaggaactc ggacttgtag cgcgagctga gcgccagctg ctccgccttg tcctccagcg
     5101 ccgccttggc ctgctccacc tcgcggttct tgagctccac ctggcgcatc tgctcggaga
     5161 gctggcgcgc cttctcctgc agctgctcgt tggcgccgcg cagctcctcc tgcttgctct
     5221 tcagcagggt ttccgactgc tgcaggttga gcgcctgctg ctccaggcgc acgttggtct
     5281 tctgcagctc ctgctgctgc gcctgcagct cggcggtcag cgtctgcgac tgctgcagca
     5341 ggtcctcggt gcgcatgttg gccgcgatgg tgtgcagcac cacgccgatg ctgtcggtca
     5401 gctggtcgag gaagctctgg tggatgtcgc tgaagcggtt gaacgaggcc agctcgatca
     5461 ccgccttcac ttcgccctcg aacagcaccg gcaggatcat cacgctgccc ggcgcgcccg
     5521 agcccagcgc cgagccgacg cgcaggtaat cccccggcac gctgccgagc acgatgcgct
     5581 tcttctcgta ggcgcactgc cccaccagcc cctcgccgag cgggatggag cgcggcaggc
     5641 tgtcgggaca ggcgtagctg gcgagcagct cgagctgcgc cgcgtcggcg tggccggccg
     5701 cgatgtagaa cgccgcctgc tgcgcctcca ccagcggcgc cagctccgac aggatcaggc
     5761 gcgacacggt caccaggtcg cggtgcccct gcagcatgcg cgtgaagcgc gccaggtgcg
     5821 tcttcagcca gtcctgctcg gtgttcttga gcgtggtgtc cttgaggttg acgatcatct
     5881 cgttgatgtt gtccttcagc gccgccacct cgccctgcgc ctcgaccatg atcgagcgcg
     5941 tcaggtcgcc cttggtgacc gcggtggcca cctcggcgat ggcgcgcacc tgcgtggtca
     6001 ggttggccgc cagctggttc acgttgtcgg tgaggtcgcg ccacagcccg gcggcgccgg
     6061 gcacgcgcgc ctggccgccg agcttgccct cgatgcccac ctcgcgcgcc acgtcggtga
     6121 cctggtcggc gaaggtggcg agggtctcga tcatggcgtt gatggtgtcg gcgagcgccg
     6181 ccacctcgcc cttggcctcc accgcgatct tcttcgacag gtcgccgcgc gccaccgccg
     6241 tggtcacctc ggcgatgttg cgcacctggt cggtgaggtt ggccgccatc gagttcacgt
     6301 tgtcggtgag gtccttccag gtccccgcca cgcccgacac ctgcgcctgg ccgcccagtt
     6361 tcccgtcggt gcccacctcg cgcgccaccc gcgtcacctc cgaggcgaag gcgttgagct
     6421 ggccgaccat ggtgttgacc accgtggcga tgcgcagcac atcg
//
LOCUS       DASRIE010000205         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3312301, whole genome shotgun sequence.
ACCESSION   DASRIE010000205 DASRIE010000000
VERSION     DASRIE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312301"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFB53_08730"
     CDS             complement(<1..301)
                     /locus_tag="VFB53_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZN86854.1"
                     /translation="MSEPAQLPLAARVARLYGEPMLEIPQDLYIPPDALEVILDAFEG
                     PLDLLLYLIRRENLDILDIPMAPLTRQYLAYVEAMRSRNLDLAAEYLVMAAMLMAI"
     gene            complement(301..1503)
                     /locus_tag="VFB53_08735"
     CDS             complement(301..1503)
                     /locus_tag="VFB53_08735"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZN86855.1"
                     /translation="MFETRVLSGMRPTGALHLGHYHGVLKNWVKLQNEYPCLFFVADW
                     HALTTDYEHPGRVEQNAWEMVVDWLAAGVDPNQAILFVQSHIQEHAELHLLLSMMTPL
                     GWLERVPTYKDQMEKLKDRDLATYGFLGYPLLQSADILIYRARYVPVGEDQVPHVEMT
                     REVARRFNHLYGREEGFEEKAKAAVKKMGTKKARLLNELRTKFLEGGDADALKRAQAL
                     LEEQQNLPRGDRERLSGWLEGGGRKILVEPDALLTESARLPGLDGQKMSKSYGNAIGL
                     REAPESVAKKIRTMPTDPARVKRSDPGTPEKCPVWPLHLVYSDEARRAWVQKGCTTAG
                     IGCLECKQPVIDAVLAELAPMRERAQTYLDDPTLVKSIIADGCDKARKLAVETMRDVR
                     EAMGLTYS"
     gene            complement(1508..2167)
                     /locus_tag="VFB53_08740"
     CDS             complement(1508..2167)
                     /locus_tag="VFB53_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN86856.1"
                     /translation="MNLLVQTVAIYALPVLLAITLHEAAHGYVARHFGDMTAHAQGRI
                     SLNPARHIDLVGTILVPVLILVASKLAGGAGLLFGWAKPVPVNYSALRRPRQHMVWVA
                     AAGPGANLAMALAWAALFKLADVLPANGFTPPMLLMAKAGIEVNVILMLLNLLPILPL
                     DGGRILASLLPNRLAWRYAKLEPWGFPILLLLLFTSALGVILAPMISFSYRVIGTVFQ
                     I"
     gene            complement(2193..2828)
                     /locus_tag="VFB53_08745"
     CDS             complement(2193..2828)
                     /locus_tag="VFB53_08745"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZN86857.1"
                     /translation="MAQYFAVHPTHPQPRLIRQAAEIVRRGGLIAYPTDSCYALGCHL
                     GDAAAQQRLRRVRGVDHRHHLTLMCRDLSEIAAYAIVDNVQFRLLKLATPGSYTFILR
                     ATREVPRRLQHPRRKTIGVRVPAHAAALALLEELGEPMLSATLILPQQTTPLADAPQI
                     RAALEHELDLIVDCGHCGTEPTTVIDLSGAAPAVLRQGKGALAALGRAAAL"
     gene            complement(2833..3663)
                     /locus_tag="VFB53_08750"
     CDS             complement(2833..3663)
                     /locus_tag="VFB53_08750"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HZN86858.1"
                     /translation="MNIDLHCHSNVSDGALAPAAVAQRAARNGVDVWALTDHDQLAGL
                     AEAREAARAAGMRFVDGVEISVTWRGSTIHVVGLRIDPANAALAGGLARVRGGRLERA
                     RRMAQELKDAGIDGAFEGALRHAENPAMVSRTHFARYLAEIGAVPEMKDAFRHYLVPG
                     KPGYVPHQWAALADAVGWICAAGGHAVLAHPGRYGLSGAALDALLAEFRAAGGEAIEV
                     VTGSHSPEQFRHFGAIALQQGFAASRGSDFHGPEEGAEFGTLPPLDPALRAVWRDWAL
                     "
     gene            complement(3660..>4188)
                     /locus_tag="VFB53_08755"
     CDS             complement(3660..>4188)
                     /locus_tag="VFB53_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86859.1"
                     /translation="RPPRVKRLANLEGFGMAVTQPAEAPKRYARWLDELHENGGFRPY
                     PDFSALADRLQKNNPRLSRPRAEFLAQHWGRQLPDGRVLLRGDPSHKIVYPVLYRYEE
                     TRACWQQVTARVLWMDAAQSDTLGRIGLTAEQHAERRAAFRDLRHVTVQNAGHMLHHD
                     QPAEVARLLEEFLRA"
BASE COUNT          596 a         1480 c         1488 g          624 t
ORIGIN      
        1 tgatcgccat cagcatcgcc gccatcacca ggtactcggc cgccaggtcg aggttcctgg
       61 aacgcatcgc ctccacgtac gccaggtact ggcgcgtgag cggcgccatc gggatgtcga
      121 ggatgtcgag gttttccctg cggatcaggt acagcagcag gtcgagcggc ccctcgaagg
      181 cgtcgagaat cacctcgagc gcgtcgggcg ggatgtacag gtcctgcggg atctcgagca
      241 tcggctcgcc gtacaggcgc gcgacgcggg cggcgagcgg cagctgcgcg ggctcgctca
      301 tcagctgtag gtgagcccca tcgcctcgcg cacgtcgcgc atggtttcca ccgccagctt
      361 ccgcgccttg tcgcagccgt cggcgatgat gctcttcacc agcgtcgggt cgtcgaggta
      421 ggtctgcgcg cgctcgcgca tcggcgccag ctccgccagc accgcgtcga tcaccggctg
      481 cttgcactcc aggcagccga tgcccgccgt ggtgcagccc ttctgcaccc aggcgcggcg
      541 cgcctcgtcc gagtacacca ggtgcagcgg ccagaccggg cacttctccg gcgtgcccgg
      601 gtcgctgcgc ttcacgcgcg ccgggtcggt cggcatggtg cggatcttct tcgccaccga
      661 ctcgggcgcc tcgcgcaggc cgatggcgtt gccgtaggac ttggacatct tctgcccgtc
      721 cagtcccggc agccgcgccg actcggtgag cagcgcgtcg ggctcgacca ggatcttgcg
      781 cccgcctccc tccagccagc ccgacaggcg ctcgcggtcg ccgcgcggca ggttctgctg
      841 ctcctcgagc agcgcctgcg cgcgcttgag cgcgtcggcg tcgccgccct cgaggaactt
      901 ggtgcgcagc tcgttcagca ggcgcgcctt cttggtgccc atcttcttca ccgcggcctt
      961 ggctttttcc tcgaagcctt cctcgcggcc gtacaggtgg ttgaagcggc gcgcgacctc
     1021 gcgcgtcatc tcgacgtgcg gcacctggtc ctcgcccacc ggcacgtagc gcgcgcggta
     1081 gatcaggatg tcggccgact gcagcagcgg atagccgagg aagccgtagg tggccagatc
     1141 gcggtccttg agcttctcca tctggtcctt gtaggtcggc acgcgctcca gccagcccag
     1201 cggcgtcatc atcgacagca gcaggtgcag ctcggcgtgc tcctggatgt gcgactgcac
     1261 gaacaggatc gcctggttcg ggtcgacgcc ggcggcgagc cagtccacca ccatctccca
     1321 ggcgttctgc tccacgcgcc ccgggtgctc gtaatcggtg gtgagcgcgt gccagtcggc
     1381 gacgaagaac aggcaggggt attcgttctg cagcttgacc cagttcttca gcacgccgtg
     1441 gtaatggccg aggtgcagcg cgccggtggg gcgcatgccg gagagaacgc gggtctcgaa
     1501 catcgcgcta gatctggaat accgtgccga tgacccggta ggaaaaactg atcatcggcg
     1561 ccaggatcac gcccagcgcg ctggtgaaca gcagcaggag caagatgggg aaaccccagg
     1621 gctccagctt ggcgtagcgc caggcgagcc ggttcggcag caggctggcg aggatcctgc
     1681 cgccgtcgag cggcaggatg ggcagcagat tgagcagcat caggatcacg ttcacctcga
     1741 tgccggcctt cgccatcagc agcatcggcg gcgtgaagcc gttggcgggc agcacgtcgg
     1801 caagcttgaa caacgcggcc caggcgagcg ccatcgcgag gttggcgccg ggaccggccg
     1861 ccgcgaccca caccatgtgc tgccgtgggc ggcgcagcgc ggagtagttg accggcacgg
     1921 gcttggccca gccgaacagc aagccggcgc cgccggccag cttgctcgcc accaggatca
     1981 ggaccggcac caggatcgtc ccgaccagat cgatgtggcg cgcgggattg aggctgatgc
     2041 gcccctgggc gtgcgcggtc atgtcgccga agtggcgcgc gacgtagccg tgggcggcct
     2101 cgtgcagcgt gatcgcgagc agtaccggca gcgcgtagat cgcgaccgtc tgtacgagca
     2161 gattgatgtc catcgggctg cggcgaatct tatcagagcg ccgccgcgcg gccgagcgcg
     2221 gccagcgcgc ccttgccctg gcgcagcacc gccggcgccg cgccgctcaa gtcgatcacc
     2281 gtcgtcggtt ccgtgccgca gtggccgcag tccacgatca ggtcgagctc gtgctcgagc
     2341 gcggcgcgga tctgcggcgc gtcggcaagc ggcgtcgtct gctgcggcag gatcagcgtc
     2401 gcggacagca tcggctcgcc cagctcttcg agcagcgcca gcgccgccgc gtgcgcgggc
     2461 acgcgcacgc cgatggtctt gcgccgcggg tgctgcagcc ggcgcgggac ttcgcgcgtc
     2521 gcgcgcagga tgaaggtgta gctgccgggc gtggccagct tcagcaggcg gaactgcacg
     2581 ttgtcgacga tggcataggc tgcgatctcg gacaggtcgc ggcacatcag cgtcaggtgg
     2641 tgccggtggt cgacgccgcg cacgcgccgc aggcgctgct gcgcggcggc gtcgcccagg
     2701 tggcagccca gcgcatagca ggagtccgtc ggataggcga tcaggccgcc gcggcgcacg
     2761 atttccgccg cctggcggat caggcgcggc tgcgggtggg tcggatggac cgcgaagtat
     2821 tgcgccaagc gctcagagcg cccagtcgcg ccacaccgcc cgcagcgccg ggtcgagcgg
     2881 cggcagcgtg ccgaactcgg cgccctcctc cgggccgtgg aaatccgagc cgcgcgaggc
     2941 ggcgaagccc tgctgcaggg cgatcgcacc gaagtggcgg aattgctccg gcgaatggct
     3001 gccggtcacc acctcgatcg cctcgccgcc cgccgcgcgg aactccgcca gcagcgcgtc
     3061 cagcgcagcg cccgacaggc cgtagcgccc gggatgcgcg agcaccgcgt gcccgcccgc
     3121 ggcgcaaatc cagcccacgg catcggcgag ggccgcccac tggtggggaa cgtagccggg
     3181 cttgccgggc accaggtagt ggcggaatgc gtccttcatc tccggcaccg cgccgatctc
     3241 ggccaggtag cgcgcgaaat gggtgcggct gaccatcgcc gggttctccg cgtgccgcag
     3301 cgcgccctcg aaggcgccgt cgatgccggc atccttcaat tcctgcgcca tgcggcgcgc
     3361 gcgctcgagg cgcccgccgc gcacgcgcgc caggccgccg gcaagcgccg cgttcgccgg
     3421 atcgatgcgc aggccgacca catggatcgt gctgccgcgc caggtgaccg agatctccac
     3481 gccgtcgacg aagcgcatgc cggctgcgcg cgccgcctcg cgcgcctcgg cgagcccggc
     3541 gagctggtcg tgatcggtga gcgcccagac gtcgacgccg ttgcgcgccg cgcgctgcgc
     3601 gacggcggcc ggcgccaggg cgccgtccga gacgttcgaa tggcaatgca ggtcgatgtt
     3661 caagcgcgca ggaactcctc gagcaggcgc gccacttccg ccggctggtc gtggtgcagc
     3721 atatgccctg cattctgcac cgttacgtgc cgcaggtcgc ggaacgcggc gcggcgctcg
     3781 gcgtgctgct ccgcggtcag gccgatgcgc cccagcgtgt cggactgcgc ggcgtccatc
     3841 cacagcacgc gcgccgtcac ctgctgccag caggcgcgcg tctcctcgta gcgatacagc
     3901 accgggtaga cgatcttgtg cgacggatcg ccgcgcagca gcacgcgccc gtcgggcagc
     3961 tggcggcccc agtgctgcgc caggaactcg gcgcgcgggc gcgacaggcg cgggttgttc
     4021 ttctgcagcc ggtcggcgag cgcgctgaaa tcgggatagg gccggaagcc gccgttctcg
     4081 tgcagctcgt ccagccagcg cgcgtagcgc ttgggcgctt cggcgggttg cgtgaccgcc
     4141 atgccgaagc cttccaggtt cgccagccgc ttcaccctcg gcggccgc
//
LOCUS       DASRIE010000206         5238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2537832, whole genome shotgun sequence.
ACCESSION   DASRIE010000206 DASRIE010000000
VERSION     DASRIE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537832"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFB53_08760"
     CDS             <1..253
                     /locus_tag="VFB53_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN86860.1"
                     /translation="VVHSFGPAQWAAMLYLGAFAGALGFYLWVYALQHATPTRVANTI
                     TVSPIAAALLAAVLIGEPIGLSLVVGVIAVGVGIWIAST"
     gene            259..387
                     /locus_tag="VFB53_08765"
     CDS             259..387
                     /locus_tag="VFB53_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86861.1"
                     /translation="MNFRTGEPAQAAKVEDGDAAVAGTNRAVALELLQRGVGARPA"
     gene            complement(400..2199)
                     /locus_tag="VFB53_08770"
     CDS             complement(400..2199)
                     /locus_tag="VFB53_08770"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN86862.1"
                     /translation="MAEEGAAGYLAGRIDALRENLVSLAAALPGLPAELARMRELLAG
                     EWEARGPGGTLALIAGFLLLGFAVEWVYRYSTPRGDVSKRLRAIGLRVARDAGAVAAF
                     AFGSAAVFLAFEWPPRTREAVVGFLVAFILLRITITASRTLLSPRDAPLRILPADDAT
                     AEFWHRRIVLFASWFAFGWVVRGWLLLLGMEPAALRLVVLTLGLGLLAILVEAFWRRS
                     RLSAVYFVALWLIWVTAAFGLMWLALIVAALPVAMRLAQQGVDHVLRPEGAAVAGARS
                     VAAAAIGRALRALLVTGAALLLVHAFGLDVDALAATDSMWMRLVRGAVHALVIVLVAD
                     VLWLVIASLIDQKLASADEEGNTRLRTLLPILRGTMAIVLIVMTVLMALSALGVEVGP
                     LLASAGVVGLALGFGAQTLVRDLFSKFFYLLDDAFRVGEYIQSGSYKGTVEHLGARSI
                     RLRHHRGPVYTIPYGELGAVQNQSRDWVIDKLTIGLAYDSDLDKAKKLIKKIGQELAQ
                     NPEFAPNILEPLKMQGVEQFGDFAIQIRMKMKTKPGQQFTIRRKAYAMIKAAFAANGI
                     KFAQPTVQVAGGGDVAAAAARQALELNKAPTSG"
     gene            complement(2244..2375)
                     /locus_tag="VFB53_08775"
     CDS             complement(2244..2375)
                     /locus_tag="VFB53_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86863.1"
                     /translation="MKTIRLAICIALGLASLHGCNATTGGPQTRSQASAPFPYNSPY"
     gene            2464..4122
                     /locus_tag="VFB53_08780"
     CDS             2464..4122
                     /locus_tag="VFB53_08780"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZN86864.1"
                     /translation="MNGEEVRALKSIRFDPGVLRAALLVGVGYYVAARLGFAFTLQPH
                     PISTLWPPNALLMAALLLTPARAWWVLLSAALPAHLAAELQSGVPLAMVLGWFASNCS
                     EALIGAGLVRAFVPAPLRLDSLRNAALFLLCGALAAPLASSFLDAAMVKLIGFGDSDY
                     WTLVRTRASSNVLADLTVVPLVLTWATFRLERLRDAPPRRYVEVAAVFLGVLATSLVA
                     FDLPLLDANAAPALFYAPLPFLLWAAVRLGPTGAASALALMVVVTIWGAVHGLGPFTG
                     GAPQDTARDLQFFLISVAVPLLLLAVVLEERTRAEREAREQRLQLAHLSRVAMLGELS
                     GGIAHELNQPLTAILANAQAAQHLLAAKRLGQEEMGEILRDIIAADQRASEVIRRLRA
                     LFKKGETQFQRLDANELVAEVLSITRGDLVMRGIETVTELAPAPPAIEGDRVQLEQVM
                     LNLVLNACEAMSAGTPLGRRLTVRTQLLADGVQISFADRGPGFHPGEYEKMFTPFHTT
                     KAQGLGLGLSISRSIIDAHGGRLWGTALPGRGATFFIVLPPRPA"
     gene            4186..4566
                     /locus_tag="VFB53_08785"
     CDS             4186..4566
                     /locus_tag="VFB53_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86865.1"
                     /translation="MPSVAIVDDEEPIRKALRRLLRASGIEAESYASGQEFLDAIAER
                     RPDCLVLDLHMSGMGGLEVLRSLQSVKHKVPTVIITAHDEPETREQCLAAGACAYLRK
                     PLEDRLLLNAISAAVRTRAVPLKR"
     gene            4569..>5238
                     /locus_tag="VFB53_08790"
     CDS             4569..>5238
                     /locus_tag="VFB53_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN86866.1"
                     /translation="MLLRVLLLCIVFPVAAGAQTLDKIRKSGTITLGYIDGAAPFSYA
                     DGNGEAQGYSVELCRAVAAGIAAQLKRANLKTRWVKLTIQNRIDAVRQKKVDLECSTT
                     TWTLSRQQLVDFSLVTFVDGGSLLVRADSDAGRINDFNGKRIAAITGTTTDKALRAAL
                     ARLVVKAEVVPIKTRDEGLGLLSQGKVDALASDRTSLIGLVVMSGGKSGTFKLSDQDF
                     SIEQY"
BASE COUNT          784 a         1872 c         1810 g          772 t
ORIGIN      
        1 tgtggttcat tccttcggcc ccgcgcagtg ggccgcgatg ctgtacctcg gcgcgttcgc
       61 cggcgcgctc ggtttctatc tctgggtgta cgcgctgcag cacgcgacgc cgacgcgcgt
      121 cgcgaacacg attaccgtga gcccgatcgc cgccgcgctg ctcgccgcgg tcctgatcgg
      181 cgagccgatc ggcctcagcc tcgtcgtcgg cgtcatcgcg gtcggcgtgg gaatctggat
      241 tgcttccacc taataggcat gaacttccgg accggcgagc cggcgcaggc cgccaaggtc
      301 gaggatggtg atgctgcggt agccggcacg aaccgggccg tcgcgctcga gcttcttcag
      361 cgcggcgttg gtgcgcggcc ggcgtgatcg cctaggacat cagcccgaag tcggcgcctt
      421 gttcagctcg agggcctggc gcgccgccgc ggccgcgacg tcgccgccgc ccgcgacctg
      481 caccgtcggc tgggcgaact tgatgccgtt ggcggcgaac gccgccttga tcatggcgta
      541 ggccttgcgg cggatggtga actgctggcc gggcttggtc ttcatcttca tgcggatctg
      601 gatcgcgaag tcgccgaact gctccacgcc ctgcatcttg agcggctcga ggatgttggg
      661 cgcgaattcg ggattctgcg ccagctcctg gccgattttc ttgatcagct tcttcgcctt
      721 gtccaggtcg gaatcgtagg ccaggccgat ggtgagcttg tcgatcaccc agtcgcggct
      781 ctggttctgc accgcgccga gctcgccgta gggaatggtg tacaccggcc cgcggtggtg
      841 gcgcaggcgg atcgagcgcg cgccgaggtg ctcgaccgtg cccttgtagc tgccgctctg
      901 gatgtactcg ccgacgcgga acgcgtcgtc gagcaggtag aagaacttgc tgaacagatc
      961 gcgcaccagg gtctgcgcgc cgaagccgag cgccaggccg accacgccgg cgctggcgag
     1021 cagcggcccg acttcgacgc cgagcgccga aagcgccatc aggacggtca tcacgatcag
     1081 cacgatggcc atcgtgccgc gcaggatcgg cagcagcgtg cgcagccggg tattgccctc
     1141 ctcgtcggcc gaggcgagct tctggtcgat gagcgaggcg atcaccagcc agagcacgtc
     1201 ggccaccagc acgatgacca gcgcgtgcac cgcgccgcgc accaggcgca tccacatgct
     1261 gtcggtcgcg gcgagcgcgt ccacgtcgag gccgaaagcg tgcaccagca gcagcgccgc
     1321 gccggtcacc agcagcgcgc gcagcgcgcg gccgatcgcg gccgcggcca cgctgcgcgc
     1381 gccggcaacc gccgcgcctt ccgggcgcag cacgtggtcc accccctgct gcgcgagccg
     1441 catcgcgacc ggcagggcgg cgacgatcag cgccagccac atcaggccga acgccgccgt
     1501 cacccagatc agccacagcg ccacgaagta gaccgcggac aggcgcgagc ggcgccagaa
     1561 cgcttccacc aggatcgcga gcaggcccag gccgagggtc aggaccacca ggcgcagcgc
     1621 ggccggctcc atgccgagca gcaggagcca cccgcggacc acccagccga aggcgaacca
     1681 gctcgcgaac aggacgatgc gccggtgcca gaactccgcc gtggcgtcat ccgccggcag
     1741 gatgcgcagg ggcgcgtcgc gcggcgagag gagcgtacga ctcgcagtga tcgtgattcg
     1801 caaaagaata aaagcgacca gaaagccgac caccgcctcg cgcgtgcgcg gcggccattc
     1861 gaaggcgagg aacaccgcgg cgctgccgaa cgcgaaggcg gcgacggccc cggcgtcgcg
     1921 ggccacgcgc aggccgatcg cgcgcaaacg cttggaaaca tcccccctgg gggtgctgta
     1981 tctgtaaacc cactcgaccg cgaagccgag gagcaggaag ccggcgatca gcgcgagggt
     2041 gccgcccggg ccccgggcct cccactcgcc ggcgagcagc tcgcgcatgc gcgcgagctc
     2101 cgcgggcaac ccgggcaacg ccgcggcgag cgacaccagg ttctcgcgca gcgcgtcgat
     2161 ccggccggcc agatacccgg cggcgccttc ttcagccatc ccttgcctcc cgcggtcatt
     2221 ttcccccgat gcgggggaaa ccgctaatag ggggagttat acgggaacgg ggccgacgct
     2281 tgcgagcgcg tctgggggcc gccggtcgtg gcgttgcaac cgtgcagcga ggcgagcccg
     2341 agcgcgatgc agatcgcgag gcgtattgtc ttcatcgggc aattcttgac ccgccgccgc
     2401 agtaccgcca ttggaccttg gtcccatccg cggagtcgtg tagcctcctc gcagggaaat
     2461 gctttgaacg gcgaggaagt ccgggcactc aagtccatcc gcttcgaccc cggcgtgctg
     2521 cgagccgcgc tgctggtcgg cgtcggctac tacgtcgcgg cgcgcctcgg cttcgccttc
     2581 acgctgcagc cgcacccgat ctcgacgctg tggccgccga acgcgctgct gatggcggcg
     2641 ctgctgctga cgccggcgcg cgcctggtgg gtgctgctgt ccgcggcgct gccggcgcac
     2701 ctcgcggccg agctgcagag cggcgtgccg ctcgccatgg tgctcggctg gttcgccagc
     2761 aactgcagcg aggccctgat cggcgccggc ctggtgcgcg ccttcgtgcc cgcgccgctg
     2821 cgcctggatt cgttgcgcaa cgcggcgctg ttcctgctgt gcggcgcgct cgccgcgccg
     2881 ctcgcttcct cgtttctcga cgccgcgatg gtgaagctga tcggcttcgg cgacagcgac
     2941 tactggacgc tggtgcgtac gcgcgcctcc tcgaacgtgc tcgccgacct gacggtggtg
     3001 ccgctggtcc tcacctgggc gacgttccgc ctcgagcgcc tgcgcgacgc gccgccgcgg
     3061 cgctacgtcg aggtcgcggc ggtgtttctc ggcgtgctgg cgacgagcct ggtggcgttc
     3121 gacctgccgc tgctcgacgc caacgccgcg cccgcgctgt tctacgcgcc gctgccgttc
     3181 ctgctgtggg ccgcggtccg cctcgggccg acgggagctg cgagcgcgct cgcgctgatg
     3241 gtggtggtga cgatctgggg cgcggtgcac ggcctggggc cgttcaccgg cggcgcgccg
     3301 caggacacgg cgcgcgacct gcagttcttc ctgatctcgg tcgcggtgcc gctgctgctg
     3361 ctcgcggtgg tgctcgaaga gcgcacgcgc gccgagcgcg aggcgcgcga gcagcgcctg
     3421 cagctcgcgc acctctcccg ggtggcgatg ctcggcgagc tctccggcgg gatcgcgcac
     3481 gagctgaacc agccgctcac cgcgatcctc gccaacgcgc aggcggcgca gcatctcctc
     3541 gcggcgaagc gcctcggcca ggaggagatg ggcgagatcc tgcgcgacat catcgccgcc
     3601 gaccagcgcg ccagcgaagt gatccggcgc ctgcgcgcgc tgttcaagaa gggcgagacc
     3661 cagttccagc gcctcgacgc caacgagctg gtggccgagg tcctgagcat cacgcgcggc
     3721 gacctggtga tgcgcggcat cgagacggtg accgagctcg cgccggcgcc gccggcgatc
     3781 gagggcgacc gcgtgcagct cgagcaggtg atgctcaacc tggtgctcaa cgcgtgcgag
     3841 gcgatgagcg cggggacgcc gctcggccgg cgcctcaccg tgcgcaccca gcttctcgcg
     3901 gacggggtgc agatcagctt cgccgaccgc gggcccggct tccaccccgg cgagtacgaa
     3961 aagatgttca ccccgttcca caccaccaag gcgcaggggc tcggcctggg gctgtcgatc
     4021 tcgcgctcga tcatcgacgc ccacggcggg cggctgtggg gaaccgcgct gcccggccgc
     4081 ggcgccacct tcttcatcgt gctgccgcct cggccggcgt aagaccttgg tccaataaga
     4141 ccttggtcca attactgcga cccgcccgac ctgctccaat ggggcgtgcc gtccgtcgcg
     4201 atcgtcgatg acgaggagcc gatccgcaag gccctgcgtc gcctgctgcg cgcctcggga
     4261 atagaggccg agagctacgc cagcgggcag gaattcctcg acgccatcgc cgagcggcgg
     4321 cccgattgcc tggtgctcga cctgcacatg tccggcatgg gcggcctgga agtgctgcgc
     4381 tcgctacaat ccgtgaagca caaggtaccc accgtgatca tcaccgccca cgacgagccc
     4441 gaaacccgcg agcagtgcct ggccgccggc gcctgcgcct acctgcgcaa gccgctcgag
     4501 gaccggctgc tcctcaacgc catctcggcc gcggtgcgca cgcgcgccgt ccccctgaag
     4561 aggtgatcgt gctgcttcga gtcttgctgt tgtgcatcgt attcccggtt gccgcgggcg
     4621 cgcagacgct cgacaagatc cgcaagagcg gcaccatcac gctcggctac atcgacggcg
     4681 cagcgccttt ctcgtacgcc gacggcaacg gcgaggcgca gggctactcg gtcgagctgt
     4741 gccgcgcggt ggccgccggc attgcggcgc agctcaagcg cgccaacctc aagacgcgct
     4801 gggtcaagct caccatccag aaccgcatcg acgcggtgcg ccagaagaag gtcgacctcg
     4861 agtgcagcac caccacctgg acgctttcgc gccagcagct ggtcgacttc agcctggtca
     4921 ccttcgtcga cggcggcagc ctgctggtgc gcgccgacag cgacgccggc cgcatcaacg
     4981 acttcaacgg caagcgcatc gcggcgatca ccggcaccac caccgacaag gcgctgcgcg
     5041 cggcgctggc gcggctcgtg gtcaaggccg aagtggtgcc gatcaagacc cgcgacgagg
     5101 gtctcgggct gctcagccag ggaaaggtcg acgcactcgc ctccgaccgc acctcgctta
     5161 tcggcctggt ggtgatgagc ggcggcaaga gcggcacctt caagctgagc gaccaggact
     5221 tctcgatcga gcagtacg
//
LOCUS       DASRIE010000207         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_449762, whole genome shotgun sequence.
ACCESSION   DASRIE010000207 DASRIE010000000
VERSION     DASRIE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449762"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFB53_08795"
     CDS             complement(<1..694)
                     /locus_tag="VFB53_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86867.1"
                     /translation="MLELKDLHAFYGKSHILHGVDMRVGEGEIVSLLGRNGSGRSTTV
                     KTVMGLVDGQGSVRWRDRPVLGLKAFEIAQLGIGYVPENRDIFPRLTVHQNLLLGEKR
                     GRKNPRWTAADMYRMFPRLQERSNVEAGVLSGGEQQMLTLCRTLMGDPDLIMIDEPTE
                     GLAPKIVELVADYLKALERRGVSVLLVEQKLTIALEISERCYVMGHGRIVFEGTPADL
                     RANAYVRKEWLEV"
     gene            complement(709..1452)
                     /locus_tag="VFB53_08800"
     CDS             complement(709..1452)
                     /locus_tag="VFB53_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN86868.1"
                     /translation="MAFGKTQIIRGASLAVAPGERVAIIGPNGAGKSTLFNLISGRFP
                     PTSGEILLNGRRIDGKKPFEINRLGLSRSFQITNIFGRLSVFENLRCGVLWTLGYRYS
                     FWHRLSRLADANERAEQLMEMIKLEKKRDTLATNLTYAEQRALEIGITIAGGADVVLL
                     DEPTAGMSKSETRRFIELIREVTVGKTLLTVEHDMGVVFGLADKIAVLVYGDVIAFDR
                     PAAVRANPKVQEAYLGSALADQQAAEARP"
     gene            complement(1488..2861)
                     /locus_tag="VFB53_08805"
     CDS             complement(1488..2861)
                     /locus_tag="VFB53_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86869.1"
                     /translation="MSAIAHPTTAPPAATAPAAPPLAAPRRAGRRPVYRFRPYNVGRV
                     VVWGGFAVLLAVAPLVFSSGLALTILSQMGIAIVACLSYNMLLGQGGMLSFGHAVYTG
                     LASFVCIHLLNAIGGGLALPVSLVPVAGGVAGMLFAVVLGFVTTRKAGTTFAMITLGI
                     GELVFSMSLMVPEFFGGEGGVQANRMVGKPFLGLTFGPQIQVYGLLAVYTFLCTAAMF
                     AFTRTPLGRMLNAVRDNPERVEFIGYSTQRVRYLAFIAAGFFAGVAGGMYALNFEIAT
                     AEVVGAFRSGSYLFFTFLGGATFFFGPIIGGVLMVLAFVLFSEFTKAWLLYLGFVFLF
                     MVMYAPGGIASLVMMNLRVAAFGKLRPLLAGYLALGGAALVVLAGAGALVEMVYHLQL
                     DTALGGTLRFLGATLDTARADSWIGALFVMLVGVGLFELSRREFARQWGEAQGQIEAE
                     IRRREAM"
     gene            complement(2861..>3603)
                     /locus_tag="VFB53_08810"
     CDS             complement(2861..>3603)
                     /locus_tag="VFB53_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN86870.1"
                     /translation="TFGLSFVILELVQLVWGRTAVEFQPPAALQGPAFTLVNSSVQGL
                     ALVWGAAPAAACSAADAAVRVVCSPFPATRGFMMLVALLMLLALWLMLTRTRIGLVIQ
                     SALTHPDMVEALGHNVPRVFMLVFGAGAALAGLAGVIGGSTFVTEPAMAATVGSVIFV
                     VVVVGGMGSLAGAFLASVLIGVIQTFAVAVDVSVSSLLGSLGLGSGASTNTFMRLTLS
                     QVAPILPYLFLVLILILRPRGLLGTRES"
BASE COUNT          606 a         1279 c         1263 g          455 t
ORIGIN      
        1 acacctcgag ccactccttg cgcacgtacg cgttggcgcg cagatccgcc ggcgtcccct
       61 cgaacacgat gcgcccgtgg cccatgacgt agcagcgctc cgagatctcc agcgcgatgg
      121 tgagcttctg ctccaccagc agcacggaca cgccgcggcg ctcgagtgcc ttcaggtagt
      181 cggcgaccag ctcgacgatc ttcggcgcga ggccctcggt gggctcgtcg atcatgatca
      241 ggtcggggtc gcccatcagc gtgcggcaca gcgtgagcat ctgctgctcg ccgcccgaga
      301 gcacgccggc ctcgacgttg gagcgctcct gcagccgcgg gaacatgcgg tacatgtcgg
      361 ccgcggtcca gcgcgggttc ttccggccgc gcttctcgcc gagcagcagg ttctggtgca
      421 ccgtgaggcg cgggaagatg tcgcggttct ccggcacgta gccgatgccg agctgcgcga
      481 tctcgaaggc cttcagcccg aggaccggcc tgtcgcgcca gcgcaccgag ccctgcccgt
      541 cgaccagccc catgaccgtc ttcaccgtgg tggaccggcc ggagccgttg cgccccagca
      601 ggctcacgat ctcgccctcg cccacgcgca tgtcgacgcc gtgcaggatg tggctcttgc
      661 cgtagaaggc gtgcaggtcc ttcagttcca gcagggcggc cgggggggtc atggccgggc
      721 ctccgccgcc tgctggtcgg ccagcgccga gcccaggtag gcctcctgca ccttgggatt
      781 ggcgcgcacc gccgcgggcc ggtcgaaggc gatgacgtcg ccgtagacca gcacggcgat
      841 cttgtcggcc aggccgaaca ccacgcccat gtcgtgctcc accgtgagca gcgtcttgcc
      901 gacggtgacc tcgcggatca gctcgatgaa gcgccgcgtc tcgctcttgc tcatgccggc
      961 ggtgggctcg tcgagcagga cgacgtcggc cccgcccgcg atcgtgatgc cgatctccag
     1021 cgcgcgctgc tcggcgtacg tgaggttggt ggccagcgtg tcgcgcttct tctcgagctt
     1081 gatcatctcc atcagctgct cggcgcgctc gttggcgtcg gccaggcgcg acagccggtg
     1141 ccagaacgag tagcggtagc cgagcgtcca cagcacgccg cagcgcaggt tctcgaacac
     1201 cgacagccgg ccgaagatgt tggtgatctg gaagctgcgc gacaagccca ggcggttgat
     1261 ctcgaagggc ttcttgccgt cgatgcgccg cccgttgagc aggatctcgc ccgaggtggg
     1321 gggaaagcgg ccgctgatga ggttgaagag cgtggacttg cccgcgccgt tcggtccgat
     1381 gatggccacc cgctcgcccg gcgcgaccgc caggctcgcg ccgcggatga tctgcgtctt
     1441 gccgaacgcc atgccgacgt ccttcagctc gatggcggcc acggcgctca catcgcctcc
     1501 ctgcgccgga tctcggcctc gatctgcccc tgggcctcgc cccactggcg cgcgaactcg
     1561 cgccgcgaga gctcgaacag gcccacgccc acgagcatga cgaagagcgc gccgatccag
     1621 ctgtcggcgc gcgcggtgtc cagcgtggcg ccgaggaagc gcagcgtgcc gccgagcgcc
     1681 gtgtccagct gcaggtggta gaccatctcg acgagcgcgc ccgcgcccgc cagcacgacc
     1741 agcgccgcgc cgcccagcgc caggtagccc gcgagcagcg gccgcagctt gccgaatgcc
     1801 gccacgcgca ggttcatcat caccaggctg gcgatgccgc cgggggcgta catcaccatg
     1861 aagaggaaga cgaagccgag gtagagcagc cacgccttgg tgaactcgga gaacagcacg
     1921 aaggcgagca ccatcagcac gccgccgatg atgggaccga agaagaaggt ggcgccgccg
     1981 aggaaggtga agaacaggta gctgccggag cggaacgcgc ccaccacctc ggcggtggcg
     2041 atctcgaagt tgagcgcgta catcccgccg gccacgccgg cgaagaagcc ggcggcgatg
     2101 aaggcgaggt agcgcacgcg ctgcgtgctg tagccgatga actccacgcg ctccgggttg
     2161 tcgcgcaccg cgttcagcat gcggccgagc ggcgtgcgcg tgaaggcgaa catcgccgcg
     2221 gtgcagagga aggtgtagac ggcgagcagc ccgtacacct ggatctgcgg cccgaaggtg
     2281 agcccgagga acggcttgcc gaccatgcgg ttggcctgca cgccgccttc gccgccgaag
     2341 aactccggca ccatgagcga catcgagaac accagctcgc cgatgccgag cgtgatcatc
     2401 gcgaacgtgg tgccggcctt gcgggtggtg acgaagccca gcaccaccgc gaacagcatc
     2461 cccgccacgc cgccggccac cggcaccagg ctcaccggca gcgccaggcc gccgccgatg
     2521 gcgttgagca ggtggatgca cacgaacgac gcgagccccg tgtacaccgc gtgcccgaag
     2581 ctcagcatgc cgccctgccc cagcagcatg ttgtacgaca ggcaggcgac gatggcgatg
     2641 cccatctgcg acaggatggt gagcgccagg ccgctggaga acacgagcgg cgccaccgcg
     2701 agcagcacgg cgaagccgcc ccacaccacg acgcggccca cgttgtacgg gcggaagcgg
     2761 tagaccggcc ggcgcccggc gcgccggggc gccgcgagcg gcggcgcggc gggcgccgtg
     2821 gcggcgggcg gcgcggtcgt ggggtgcgcg atggccgaca tcaggactcc cgcgtgccga
     2881 gcaggccgcg cgggcgcagg atgaggatca gcacgaggaa gaggtacggc aggatcggcg
     2941 ccacctgcga cagcgtgagc cgcatgaagg tgttggtgga agcgccgctg ccgagcccca
     3001 ggctgccgag caggctggac accgacacgt ccaccgccac cgcgaaggtc tggatcacgc
     3061 cgatcagcac cgaggcgagg aaggcgccgg ccagcgagcc catgccgccc acgaccacca
     3121 ccacgaagat caccgagccc accgtcgccg ccatcgccgg ctcggtgacg aaggtgctcc
     3181 cgccgatcac gccggcgagt cccgcgagcg ccgcgccggc gccgaagacc agcatgaaca
     3241 cgcgcggcac gttgtggccg agcgcctcca ccatgtccgg atgggtgagt gcgctctgga
     3301 tcaccaggcc gatgcgcgtg cgcgtgagca tgagccacag cgccagcagc atgagcagcg
     3361 ccaccagcat catgaagccg cgggtggccg ggaacggcga gcacacgacg cgcacggcgg
     3421 cgtcggcggc gctgcacgcg gcggccggcg cggcgcccca caccagcgcg aggccctgca
     3481 ccgacgagtt gaccagcgtg aacgccggcc cctgcagcgc ggcgggcggc tggaactcga
     3541 ccgcggtgcg gccccacacg agctgcacca gctcgaggat cacgaacgac aggccgaagg
     3601 tga
//
LOCUS       DASRIE010000208         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2830411, whole genome shotgun sequence.
ACCESSION   DASRIE010000208 DASRIE010000000
VERSION     DASRIE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830411"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="ccoN"
                     /locus_tag="VFB53_08815"
     CDS             complement(<1..1109)
                     /gene="ccoN"
                     /locus_tag="VFB53_08815"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HZN86871.1"
                     /translation="METSYNYKVVRQFALATVLWGVVGMAAGVFIAAQLLWPQLNFDI
                     PWLSYGRLRPLHTNAVIFAFGGCALFATSYYVVQRTSHARLFCDRLAAFTFWGWQAVI
                     VLAAVTLPMGITSGKEYAELEWPIDLLIAAVWVSYAVVFFGTLFKRKVAHIYVANWFF
                     GAFIITVALLHIVNSAVVPATLWKSYSAYSGAQDAMVQWWYGHNAVGFFLTAGFLGMM
                     YYFVPKQAGRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPEWTQSLGMVFSLILL
                     APSWGGMINGIMTLSGAWHKLREDPILKFLIVSLSFYGMSTFEGPMMSIKTVNALSHY
                     TDWTVGHVHSGALGWVGLISMGSIYYLIPRLYGRA"
     gene            1379..2956
                     /locus_tag="VFB53_08820"
     CDS             1379..2956
                     /locus_tag="VFB53_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN86872.1"
                     /translation="MAAQAEWIGEAIGDGRIDMRGGELDLFMLGALGTTPQVSDIGVV
                     DASGRARRWMRGARQAVVADWSERTEVREWLEAGRTQPGSAWRPLLWQPAERVPALLH
                     QTPLHRDGVFVGMLGQVVPIAKVSEDLALFGAEHEVTPFILHGADHVLGHPLLAQRRS
                     GELGPLPALGEIGDPVLERIGSPDGSAPLLGALKRAAAVRARVGDRHFLYIYRDISQA
                     GGQTWRIGVYLDPVEGGQRAEMLRLARSLAAVLAMLLGAVVIAALAARRLGRPIEALA
                     RAARLVRKGRIDEVQPLPRSRIYELDEANRSFGEMVEGLRERNMVRETLGQFVPEQVA
                     QELLAGGGRLEPIEDKATVMVCDLEEFAALTDSLGAHRAIEFLNAYFAVLVEIVERYQ
                     GVVTQFQGDAILAVFNLPIAARDHAANALRAALEIVRAADSQTFAGVRVRNRVGLSTG
                     RVVAGAVGSAGRRSYTVHGNAVNLAARLEQLNKDYGTRILLTEKTAERCPGFALRKVA
                     DATVRGYGEPLALYAAF"
     gene            complement(2974..3111)
                     /gene="ccoS"
                     /locus_tag="VFB53_08825"
     CDS             complement(2974..3111)
                     /gene="ccoS"
                     /locus_tag="VFB53_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HZN86873.1"
                     /translation="MESLYLLVPVSIALVFVSGWAFWRALDSGQFDDLERPAHDILDD
                     D"
     gene            complement(3060..>4836)
                     /locus_tag="VFB53_08830"
     CDS             complement(3060..>4836)
                     /locus_tag="VFB53_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZN86874.1"
                     /translation="DVSQLMRWASLLITLPVLAFSCQPFFAGALRELAQRRVGLDTPI
                     ALGIGFGFAASAWATLAGAGEVYFDSISMLAFLLLGARYLENAARRRAARALDPLLRW
                     SAGGKYSPGQELRIAPGERLPADGIVVEGVSSADEALLTGEGRPVAKRPGDEVVGGSV
                     NLEQPLLIRVTRSGADSRAAAIARLAERGAASKPRLVAAAERVARHLTGVIVASAAAA
                     GLYHADPWIAVAVLVVACPCALALAAPIVLTRASGALLGRGVLLTRAAALDSLERVTD
                     VVLDKTGTLTIGRVAVARTTVLGPLDAARCLELAAALEASSRHPVARAFGAPVATRIE
                     SPRNFPGLGIEATAQGRRVRIGSEPFCRALCGAPPAAAAQRDCDGSSVFLAAEDGWLA
                     AFELADSLRPAAAGFVARLKHEGLAVHLASGDRPGLSAALARALSIENAAGGMAPEDK
                     YAYVEALQRAGRVVAMVGDGLNDAPVLARADVSFAMGGGADAAQLKADVVLMNDSLDG
                     VVETLRVARRTMSLVRQNVGWALAYNAVALPLAAAGWIGPWEAALAMGASSLAVLLNA
                     LRPLGRDPEWKASTYSSRSRSPSYS"
BASE COUNT          770 a         1704 c         1732 g          630 t
ORIGIN      
        1 tcggcgcgcc cgtacagcct ggggatcagg tagtagatgc tgcccatgct gatcaggccc
       61 acccagccga gcgcgcccga gtgcacgtgg ccgacggtcc agtcggtgta gtgcgagagc
      121 gcgttcaccg tcttgatcga catcatcggc ccctcgaagg tcgacatgcc gtagaacgag
      181 agcgagacga tgaggaactt gaggatcggg tcctcgcgca gcttatgcca ggcgcccgag
      241 agcgtcatga tgccgttgat catcccgccc cacgacggcg cgagcaggat gagcgagaac
      301 accatgccga gcgactgcgt ccactcgggc agcgcggtgt agtgcaggtg gtgcggcccc
      361 gcccacatgt aggtgaagat gagcgcccag aagtgcacca ccgacaggcg gtaggagtag
      421 atcgggcgcc cggcctgctt cggcacgaag taatacatca tgccgaggaa ccccgcggtg
      481 aggaagaagc cgaccgcgtt gtgcccgtac caccactgca ccatcgcgtc ctgcgcgccg
      541 gagtaggccg agtacgactt ccacaaggtc gccggcacca ccgcgctgtt cacgatgtgc
      601 agcagcgcca ccgtgatgat gaacgcgccg aagaaccagt tggcgacgta gatatgcgcg
      661 acttttctct tgaacaaagt tccgaagaag accacggcgt acgaaaccca caccgcggcg
      721 atcagcaggt cgatcggcca ctcgagctcg gcgtactcct tcccggaggt gatccccatg
      781 gggagcgtca ccgcggcgag cacgatcacc gcctgccagc cccagaaggt gaaggccgcg
      841 aggcggtcgc agaacagcct cgcgtgcgag gttcgctgca ccacgtagta ggaggtggcg
      901 aagagcgcgc agccgccgaa cgcgaaaatc accgcgttgg tgtgcagcgg gcgcaggcgg
      961 ccgtaggaaa gccacgggat gtcgaaattg agctgcggcc acaacagctg cgccgcgatg
     1021 aatacgccgg ccgccatccc caccacgccc cacaggaccg tggcgagcgc gaactggcgc
     1081 accaccttgt agttgtacga agtctccatg agccgccatc gtaaaaagga gcggcgggcg
     1141 ccgtttgatg taggtcaaac gccatggacc gcggcggcta tcatcgcgag cactgaaacg
     1201 catttccttc gccactgtcg ccgtcgccgg cctcggcgcc ttcgtcgcgc tcaccgccgg
     1261 cgtcacgctg tacctaagcg cggcgagcgg ctttcgctcc acccaggcgc tgatcgcgca
     1321 gcaggccgag gcgctgatcg acgcgctcga gcagcgcctc gaggcgcggt tgcgcccggt
     1381 ggccgcgcag gcggagtgga tcggggaggc gatcggcgac ggacgcatcg acatgcgcgg
     1441 cggcgagctc gacctgttca tgctcggcgc gctcggcacc acgccgcagg tgtccgacat
     1501 cggcgtcgtc gacgcctcgg gccgggcgcg gcgctggatg cgcggcgcgc gccaggccgt
     1561 ggtcgcggac tggtccgaaa ggacggaggt gcgggagtgg ctggaagcgg gtcgcacgca
     1621 gccgggaagc gcatggcggc cgctgctgtg gcagccggcg gagcgcgtgc cggcgctcct
     1681 gcaccagacg ccgctgcacc gcgacggcgt gttcgtcggc atgctggggc aggtggtgcc
     1741 gatcgccaag gtctccgagg acctcgcgct gttcggcgcc gagcacgagg taacgccatt
     1801 catcctccat ggcgccgatc acgtgctcgg ccatccgctg ctcgcgcagc gccggagcgg
     1861 cgagctcggc cctttgccgg cgctaggcga aatcggcgac ccggtgctcg agcgcatcgg
     1921 ctcgccggac ggaagtgcgc cgctgctcgg cgcgctcaag cgcgcggcgg cggttcgcgc
     1981 gcgcgtcggc gacaggcact tcctctatat ctaccgcgac atcagccagg ccggcgggca
     2041 gacctggcgg atcggcgtct atctcgatcc cgtggagggc ggccagcgtg ccgagatgtt
     2101 gcggctggcg cgctccctcg ccgcagtcct ggcgatgctg ctcggcgcgg tcgtcatcgc
     2161 cgcgcttgcc gcgcgccggc tcggccggcc gatcgaagcg ctcgcgcgcg ccgcgcgcct
     2221 ggtgcgcaag ggccgcatcg acgaggtgca gccgctgccg cgcagccgca tctacgagct
     2281 cgacgaggcc aaccggtcct tcggcgagat ggtcgaaggc ctgcgcgagc gcaacatggt
     2341 gcgcgaaacc cttggccagt tcgtccccga gcaggtggcg caagagctgc tcgccggcgg
     2401 cggccgcctc gagccgatcg aggacaaggc caccgtgatg gtgtgcgacc tcgaggagtt
     2461 cgcggcgctt accgactcgc tcggcgcgca ccgcgcgatc gagttcctga acgcgtattt
     2521 cgcggtcctg gtggagattg tcgagcgcta ccagggcgtg gtgacgcagt tccagggcga
     2581 cgccatcctg gcggtgttca acctgccgat cgcggcgcgc gaccacgccg ccaacgcctt
     2641 gcgcgccgcg ctcgagatcg tgcgcgccgc cgactcccag accttcgccg gagtgcgcgt
     2701 gcgcaaccgc gtcggcctga gcaccggcag ggtggtcgcc ggcgccgtcg gctcggccgg
     2761 gcggcgaagc tacaccgtgc acggcaacgc ggtgaacctg gcggcgcgcc tggagcagct
     2821 gaacaaggac tacggcacgc gcatcctgct tacggaaaag accgccgagc gctgcccggg
     2881 gttcgcgctg cgcaaggtgg ccgatgcaac ggtccgcggc tacggcgagc ccctggctct
     2941 ctacgcagcc ttctgaccag acccaagccc gcttcagtcg tcgtccagga tgtcatgcgc
     3001 cgggcgctcg aggtcgtcga actgccccga gtcgagcgcg cgccagaacg cccagccgct
     3061 tacgaatacg agggcgatcg agaccgggac gagtaggtag aggctttcca ttcggggtcg
     3121 cgcccgagcg ggcgcagagc gttgaggagc acggcgagcg agctcgcgcc catggcgagc
     3181 gccgcttccc acgggccgat ccagccggcg gcggcgagcg gcagcgccac ggcgttgtag
     3241 gcgagcgccc agccaacgtt ctggcgcacc aggctcatcg tgcgccgcgc gacgcgcaag
     3301 gtctcgacca cgccgtcgag cgagtcattc atcagcacca cgtcggcctt gagctgcgcc
     3361 gcatccgcgc cgccgcccat ggcgaacgag acgtcggcac gcgcgagcac gggcgcatcg
     3421 ttcaagccgt cgcccaccat ggccaccacc cggcccgcgc gctgcagcgc ctcgacgtag
     3481 gcgtacttgt cctccggcgc catcccgccg gcggcgttct cgatcgacag cgcgcgcgcg
     3541 agcgccgcac tgagcccggg acgatcgccg ctcgcgaggt ggaccgcgag gccctcgtgc
     3601 ttgaggcgcg cgacgaagcc cgcggccgcg gggcgcaggc tgtcggcaag ctcgaacgcc
     3661 gcgagccagc cgtcttcggc cgcgaggaac acggacgagc cgtcgcaatc ccgctgcgcc
     3721 gctgcggcag gcggcgcgcc gcacagcgcc cggcagaagg gctcgctgcc gatgcgaacg
     3781 cgccggccct gcgcggtcgc ctcgatcccc aggccgggaa agttgcgcgg cgactcaatc
     3841 cgcgtggcga caggcgcgcc gaacgcgcgc gcgaccgggt gccggcttga agcctcaagc
     3901 gccgccgcga gctcgaggca gcgggccgcg tcgagcgggc cgaggaccgt ggtgcgcgcc
     3961 accgcgaccc ggccaatggt gagcgtgccg gtcttgtcca gcaccacgtc ggtgacgcgt
     4021 tccagcgagt cgagcgccgc ggcgcgggtc aggaggacgc cgcggccgag aagcgcaccg
     4081 ctcgcgcgcg taaggacgat cggcgcggca agcgcgagcg cgcaggggca ggctacgacc
     4141 agcaccgcga cggcaatcca tggatcggcg tggtagagcc ctgcggcggc ggcgctcgcc
     4201 acgatgacgc cggtcaggtg gcgcgccacg cgctcggccg cggccaccag gcgcggcttg
     4261 gaggcggcgc ccctttcggc gagccgcgcg atggcggcgg cgcgggaatc cgcgccgctc
     4321 cgcgtaacgc ggatcaggag gggctgctcg aggttcaccg atccgccgac cacctcgtcg
     4381 ccgggacgct tcgccactgg acggccttcg cccgtgagga gcgcttcgtc cgcgctcgag
     4441 acgccttcca ccacgatgcc gtcggcgggc aggcgctcgc cgggcgcgat gcgcagctcc
     4501 tgccccggcg agtacttgcc gcccgcgctc cagcgcagca gcggatcgag cgcgcgcgcc
     4561 gcccgccgtc gcgccgcgtt ttccaggtag cgcgcgccga gcaggaggaa tgcgagcatg
     4621 ctgatcgagt cgaagtacac ctcgccggcg ccggcgagcg tcgcccaggc gctcgccgcg
     4681 aagccgaacc cgatgccgag cgcgatcggc gtgtcgagcc cgacgcgccg ctgcgccagc
     4741 tcgcgcagcg cgccggcgaa aaagggctgg caggagaagg cgagcaccgg cagcgtgatg
     4801 aggaggctcg cccagcgcat cagctgcgag acatcc
//
LOCUS       DASRIE010000209         8803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2029133, whole genome shotgun sequence.
ACCESSION   DASRIE010000209 DASRIE010000000
VERSION     DASRIE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029133"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..652
                     /locus_tag="VFB53_08835"
     tRNA            562..652
                     /locus_tag="VFB53_08835"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:596..598,aa:Sec,seq:tca)
     gene            complement(657..1157)
                     /locus_tag="VFB53_08840"
     CDS             complement(657..1157)
                     /locus_tag="VFB53_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN86875.1"
                     /translation="MTPDEKLDEALKGTFPASDSFYLPPDTGARLKSRRMKTSLVAGI
                     AGQKSLVVDAGRCISFMGPENAVYATPSMVGDVEYAIRDFLLAYLDAGEDTVGTHVTI
                     DHLAATPRGMQVTIEFTVSAVEGRRVRCNFTVRDAQEECGRGVHERFVVDREKTRARL
                     AKKRAP"
     gene            1242..1943
                     /locus_tag="VFB53_08845"
     CDS             1242..1943
                     /locus_tag="VFB53_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN86876.1"
                     /translation="MRGLKGKTAIVTGGAAGIGKAIVERFEAEGTNVVVFDLHGKPPV
                     DITDYEAVRAAAAAAGPADILVNNAGWDRFRPFLETDPAYWQKIISINLVGAMNVLHN
                     VLPGMAARGGGKVVTIASDAGRVGSSGEAPYSACKGGLIALTKTLARELAKKGVRLNC
                     VCPGLTETAMFQEFVQGAGNPDKLREAYRRGVPLGRLGEPADIAGAVLFLSSDDADFI
                     TGQTISVSGGLTMHG"
     gene            complement(1927..2913)
                     /locus_tag="VFB53_08850"
     CDS             complement(1927..2913)
                     /locus_tag="VFB53_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZN86877.1"
                     /translation="MTDSARRQVAAWLLLCAALVFLLLVVGGATRLTRSGLSIVEWQP
                     LVGVLPPLGEADWQALFAKYRATPEFRLVNREMDLAGFQRIFWWEYAHRLLARAVGLA
                     FLLPLLWFQFRAMLERPLAWKLWGIFALGAAQGAMGWLMVQSGLVDDPKVSPLRLTAH
                     LGLALAIFSAELWLALGLLWPGQERRARLAAALPLLVFLMALSGGMVAGLRAGAAYQT
                     FPLMNGSFVPPDFLLLEPWWNNFLYNMGTVQFVHRAFFWLLLVLVPLAWWKAGRSSSA
                     TLLLCAFLLQAALGIATLLAGVPLALGAAHQAGAVLLLAAALWHAHRSSRAW"
     gene            complement(2933..3214)
                     /locus_tag="VFB53_08855"
     CDS             complement(2933..3214)
                     /locus_tag="VFB53_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86878.1"
                     /translation="MSQPIQETPADYDRSRIVERPDGYYWQERDGGREYGPFATLLEA
                     VQDMEVGGDEDAEPGETLAEAESEIGISDHVDPDTGELAEEQRPRLEDH"
     gene            complement(3267..4412)
                     /locus_tag="VFB53_08860"
     CDS             complement(3267..4412)
                     /locus_tag="VFB53_08860"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HZN86879.1"
                     /translation="MTAGGPDVLAAPVPAPGGLQRLRAYVEQNERAVAVGFFAAGFLF
                     DIVTVGRVDSWLVIGQQAAYLLVIGGVLLHMFLEQGAPAPDLAGLPRLWRWYREYRSA
                     AIHFALGALLNLYAIFYFKSSSLLVSFGFLAFLLVLLLANESRRFKSLGLHVKFALLG
                     LCCLSFAALVVPVFVGSIGLAVFLLSMFAGTLPLVFVAWWIFRRHPALFTQARRQILL
                     PLGVVLLAFLVSYLFRVIPPAPLSIPFIGVYHAVERSGDSYRLVHERPWWRIWHNGDQ
                     WFRAQPGDRVVVYFRVFSPARFADQVQMRWYWRPQARGWLLQDTIPINIVGGRELGFR
                     GYGFKTNYQPGNWKVQVETTDGREIGRVYFKLETAAAGDRIIQTDVD"
     gene            complement(4409..4645)
                     /locus_tag="VFB53_08865"
     CDS             complement(4409..4645)
                     /locus_tag="VFB53_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86880.1"
                     /translation="MKAFLGSLAQRRAQLLERSAAQRGAIAAAAADIRRATAAPLLLG
                     ASAAAALLAASPKLRGWAVRGWAVYAFVRQLAGR"
     gene            complement(4642..5001)
                     /locus_tag="VFB53_08870"
     CDS             complement(4642..5001)
                     /locus_tag="VFB53_08870"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZN86881.1"
                     /translation="MGIVQSGQALLATALELGRTRLELLSVELQELLARLVLLMIGAV
                     AAILLAALALGFGAVALVLAVPAEHRVALTAGLAIAFLVAAALIAWKVAREAKARPFA
                     ASLAELQQDAAALRQRE"
     gene            complement(5004..5294)
                     /locus_tag="VFB53_08875"
     CDS             complement(5004..5294)
                     /locus_tag="VFB53_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZN86882.1"
                     /translation="MTTVSTDQLMSDLRAVVRDAEELLRATAGQTSAQVDELRARAEA
                     SLGAARARLQEAGDDARQAAREIDEQVRANPWAAVGIAAAAGLLLGMLLGRK"
     gene            5337..8042
                     /locus_tag="VFB53_08880"
     CDS             5337..8042
                     /locus_tag="VFB53_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HZN86883.1"
                     /translation="MSGLSERHYLTPLFEPAAVAIVGATERAGSVGAVLVRNMLEAEY
                     GGALFGVNPKHKQVYGVPCFASVAKLPRRADLAVIATPAATVPDVIEQCGKAGVRSAV
                     VISAGFSEAGPAGVKLERAFLEHARRHGVRVVGPNCLGIMRPALGLNASFARGAALPG
                     SLALVAQSGAVCTAMLDWARPNKVGFSSVVSLGGSTDVDFGEIIDYLIEDAHTEHILL
                     YIEGVRSARRFVSALRAAARVKPVIVMKVGRQAAGSRAAVSHTGAIVGLDDVFDAVVK
                     RTGVVRVSSIGQMVAAAQALAAHVRPQGERLAVVTNGGGPGVMAADRAADLGIPLAEL
                     SAATIARLKPELPANWSHGNPIDLIGDAGAGRYRAAVSACLADSGVDGVLAILAPQAM
                     TEPEKSAQAVIEAAKGSSKPVLACWMGEEQTAAARRVLAEARIPVFRTPDPAVEMFGQ
                     VSAFYRNQRALLQTPGPLSVQGEPDLDAARAIVAAALKEKRSVLSEMESKAVLAAFRI
                     PVAKTVAATSAQEAGRIAQEIGFPVAMKIDSPDITHKSEVNGVRLNLGSAQAVQAAWE
                     GIVADARRLRPGARIRGVVLEPMVRRAHGRELMAGVIRDPVFGPAIVFGTGGTAVEVH
                     RDRAVALPPLNQFLIADMIRGTRVARLLGAFRNLPPVDMASLEEVLLRVSELVCELPE
                     VAELDVNPLLADEAGVIAVDARVVLRPGAPRRERYAHMAIHPYPARLVTTWQPREGPL
                     VTLRPIRPEDAEMEQAFVKGLSAQSRYFRFLDTLRELTPAMLVRFTQIDYDREMAFVA
                     TVARDGGEAEIGVCRYVANPDGASCEFAVVIADDWQRKGLGRRMLALLVDTARARGLR
                     SMIGHVLAENRGMLQLCQSLGFVVSDSPEGPMVRRATLALDAGGK"
     gene            8046..>8803
                     /locus_tag="VFB53_08885"
     CDS             8046..>8803
                     /locus_tag="VFB53_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZN86884.1"
                     /translation="MPMREGSLEAPTRHPLDWHNPAFFDEAALQKEQERVFDICHGCR
                     RCVNLCTAFPTLFDLVDNSKTMEVDGVAKADFGKVVDQCYLCDMCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAYQFKAGTMKHPVRDSQLSSTDRNGKLATIPVVVQLVNAAAKSKPL
                     RALGEKVAGVHKDAWVPEFTSRPFRRNAAMSKPHPVKDGERTPGKVVVYSTCYVNYNE
                     PGIGHDLLKLLDHNEIPYVMVEKEACCGMPKFEQG"
BASE COUNT         1362 a         3123 c         3139 g         1179 t
ORIGIN      
        1 cgatggactg gagcgcgccg tcgccgattg gcacgcggcg cagccggagt cggtgggtcc
       61 gcgtcccagg gacctgaagg gcgggctcgc ggcggcgatc gaccggctgg tggaagaacg
      121 acgcctggtg cgcgaagggc cgtatctgca tctgcccggc caccggccgg tcctgtcggc
      181 gcaggacgaa gcgctgtgga agcgcgcggc gccgctgctg gaggaaacac ccgacggcaa
      241 gccgccgcgc gtgcacgagc tcgcgccgct tttggggatg gagccgaagg cggtggccga
      301 tttcctgcag cgcgcggcgc gcgcgggacg cgtctatcgc gtggcgccca atcgattcct
      361 tctccccgcc aaggtggatt ccctggtcgg gctggcagtc gcgctcgatg aggagtcggc
      421 gggccgggga tttccggcgg cgctgtaccg cgaccgctcg ggcctggggc gcaacctcac
      481 catccaggtg ctggaattcc tcgacgaagc gggctacacg aagcgcgcgg gcgaactgcg
      541 ccgcgcgcgc aaatcctttc tggaagagca acgcccccgg tggggcgccc ggacttcaaa
      601 tccggagaga ggcgcctgac gcctctggtg ggttcgactc cttcctcttc cgccgcctat
      661 ggcgcgcgct tcttcgccag gcgcgcgcgc gtcttctccc ggtccacgac gaagcgctcg
      721 tgcacgccac gcccgcactc ctcctgcgcg tcgcgcaccg tgaaattgca gcgcaccctg
      781 cggccctcga ccgcgctcac cgtgaactcg atggtgacct gcatgccgcg cggggtggcc
      841 gccaggtggt cgatggtgac gtgggtgccc accgtgtcct cgcccgcgtc gaggtaggcg
      901 agcaggaaat cgcggatcgc gtactccacg tcgcccacca tgctgggcgt ggcgtacacg
      961 gcgttttccg ggcccatgaa ggaaatgcag cggcccgcgt ccacgacgag ggatttctgt
     1021 ccggcgatgc ccgcgaccag gctggttttc atgcgacggg attttaatcg cgcgccggtg
     1081 tccggcggca ggtagaacga gtcgctcgcc ggaaacgtcc ccttcagcgc ctcgtccagc
     1141 ttttcgtcgg gggtcatgcc cctgtgaccc ggcgcggcgc ggcgagtttc cggcttgccc
     1201 gccttgccgt tcgctgcgac catcgccgga aaggagcagc catgcgagga ctgaaaggca
     1261 agaccgccat cgtgaccggc ggcgcggccg gcatcggcaa ggcgatcgtc gagcgcttcg
     1321 aggccgaagg cacgaacgtg gtggtgttcg acctgcacgg caagccgccg gtggacatca
     1381 ccgactacga ggcggtgcgc gcggcggcgg cggccgccgg cccggcggac atcctggtca
     1441 acaacgccgg ctgggacagg ttccggccgt tcctcgagac cgacccggcg tactggcaga
     1501 agatcatttc catcaacctg gtcggcgcga tgaacgtgct gcacaacgtg ctgccgggca
     1561 tggcggcgcg cggcggcggc aaggtggtga ccatcgcctc cgacgccggg cgcgtgggct
     1621 cctcgggcga agcgccctac tcggcctgca agggcggcct catcgcgctc accaagacgc
     1681 tcgcgcgcga gctggcgaaa aagggcgtgc ggctgaactg cgtctgtccc gggctcaccg
     1741 agaccgccat gttccaggag ttcgtgcagg gcgccggcaa cccggacaag ctgcgcgagg
     1801 cctatcgccg cggcgtgccg ctcgggcgcc tgggcgagcc cgccgacatc gccggcgcgg
     1861 tgctgttcct gtcgagcgac gacgccgact tcatcaccgg ccagacgatc agcgtctcgg
     1921 gcggcctcac catgcacggc tagagcggtg cgcgtgccat agcgcagcgg cgagcagcag
     1981 caccgcgccc gcctgatgcg ccgcaccgag cgcgagcggc acgccggcga gcaaggtcgc
     2041 gatgccgagc gccgcctgca gcaggaaggc gcaaagcagc agcgtggcgg acgaggagcg
     2101 tcccgccttc caccaggcga gcggcaccag cacgagcaga agccagaaaa acgcgcggtg
     2161 cacgaactgc accgtcccca tgttgtagag aaaattgttc caccagggtt cgagcagcag
     2221 gaaatccggc ggcacgaaac tcccgttcat cagcggaaac gtctggtagg ccgcgcccgc
     2281 gcgcaggccc gccaccatgc cgcccgagag cgccatgagg aagacgagga gcggcagagc
     2341 cgcggcgagc cgcgcgcggc gctcctgccc cggccacaga agtcccagcg cgagccacag
     2401 ctcggccgaa aagatcgcca gcgccaggcc caggtgcgcc gtgaggcgca gggggctgac
     2461 tttcggatcg tccaccaggc ccgactgcac catcagccag cccatcgccc cttgcgccgc
     2521 gccgagcgcg aagatgcccc acagcttcca ggcgaggggc cgctcgagca tcgcgcggaa
     2581 ctggaaccac agcagcggca ggaggaacgc gaggcccacc gcgcgcgcga gcaggcggtg
     2641 cgcgtactcc caccagaaga tgcgctggaa acccgcgagg tccatctccc ggttgaccag
     2701 cctgaattcg ggcgtggcgc ggtacttggc gaacagcgcc tgccagtccg cctcgccgag
     2761 cggcggcagc acgccgacca ggggctgcca ctcgacgatg gacagccccg agcgcgtcag
     2821 gcgcgtcgcg ccgcccacca ccagcaggag gaagacgagc gcggcgcaca gcagcagcca
     2881 ggcggcgacc tgccgccgcg cggaatcggt catggcgcgc cgcgcggccg cttcagtgat
     2941 cttccagccg ggggcgctgt tcctccgcga gctcgccggt gtcggggtcc acgtgatccg
     3001 agatgccgat ctcggattcg gcttccgcca gcgtctcgcc cggctccgca tcctcgtcgc
     3061 cgccgacctc catgtcctgc acggcttcga ggagcgtggc gaacgggccg tattcgcggc
     3121 cgccgtcccg ctcctgccag tagtacccgt cggggcgctc cacgatgcgc gagcggtcgt
     3181 agtcggcggg tgtctcctgg atcggctggc tcatcgctta cctccgtggg cacgattcta
     3241 tgcccaggcg gcgcctggat gcgacgctaa tccacgtccg tctggatgat gcggtccccg
     3301 gcggccgccg tctccagctt gaaatacacg cggccgatct cgcggccgtc ggtggtctcc
     3361 acctgcacct tccagttgcc gggctggtag ttggtcttga agccgtagcc gcggaaaccc
     3421 agctcgcggc cgccgacgat gttgatcggg atcgtgtcct gcagcagcca gccgcgcgcc
     3481 tgcggcctcc agtaccagcg catctgcacc tggtcggcga agcgggcggg cgagaacacg
     3541 cggaagtaca ccaccacgcg gtcgcccggc tgcgcgcgga accactggtc gccgttgtgc
     3601 cagatgcgcc accacggccg ctcgtgcacc agccggtagc tgtcgccgct gcgctccacc
     3661 gcgtggtaca cgccgatgaa cgggatggac agcggcgccg gcgggatgac gcggaacagg
     3721 taggacacga ggaaggcgag cagcaccacg ccgagcggca gcaggatctg gcgccgcgcc
     3781 tgcgtgaaca gggccgggtg gcggcggaag atccaccagg cgacgaacac cagcggcagc
     3841 gtgccggcga acatcgacag caggaacacc gcgaggccga tcgagcccac gaacaccggc
     3901 accaccagcg cggcgaagga caggcagcac aggccgagca gcgcgaactt gacgtgcagg
     3961 cccagcgact tgaagcggcg cgattcgttt gccagcagga gcaccagcag gaaggcgagg
     4021 aagccgaagg acaccaggag cgaggagctc ttgaagtaga agatggcgta caggttgagc
     4081 agcgcgccga gggcgaagtg aatcgccgcc gagcggtact cgcggtacca gcgccacagc
     4141 cgcggcaggc ccgccaggtc cggcgccggc gcgccctgct cgaggaacat gtgcagcagc
     4201 accccgccga tgaccagcag gtaggccgcc tgctggccga tcacgagcca ggaatcgacg
     4261 cggccgacgg tgacgatgtc gaacaggaag ccggcggcga aaaaaccgac cgccaccgcg
     4321 cgctcgttct gctcgacgta ggcgcgcagc cgctgcagcc cgccgggcgc cggtaccggc
     4381 gccgccagca cgtcgggccc gcccgcggtc atcgtcccgc cagctgcctg acgaaggcgt
     4441 acaccgccca gcccctgacc gcccagccgc gcagcttcgg cgaggcggcg agcagcgccg
     4501 ccgccgcgct ggcgccgagg aggagcggcg cggccgtggc gcgccggatg tcggcggcgg
     4561 ccgcggcgat tgcgccgcgc tgcgccgcgg agcgctcgag cagctgcgcg cggcgctgcg
     4621 ccagcgagcc gaggaaggcc ttcattcgcg ctgcctgagc gccgcggcgt cctgctgcag
     4681 ctccgccagg ctggcggcga acgggcgcgc cttcgcctcg cgcgccacct tccaggcgat
     4741 gagcgccgcg gcgacgagga acgcgatcgc caggcccgcg gtcaacgcca cgcggtgctc
     4801 ggcggggacc gccagcacca gcgccaccgc accgaagccc agcgccagcg cggcgagcag
     4861 gatcgccgcc accgcgccga tcatcagcag caccagccgc gccagcaact cctgcaattc
     4921 cacgctgagc agctccaggc gcgtgcgccc cagctccagc gcggtcgcga gcagggcttg
     4981 cccggactgc acgatgccca tggctatttg cgaccgagga gcatgccgag cagcagcccg
     5041 gccgccgccg cgatgcccac cgcggcccag ggattcgcgc gcacctgctc gtcgatctcg
     5101 cgcgcggcct ggcgggcgtc gtcgcccgcc tcctgcagcc gcgcgcgcgc cgcgcccagc
     5161 gacgcctcgg cgcgcgcgcg cagttcatcc acctgcgcgc tggtctgccc cgcggtggcc
     5221 cgcaacagtt cctccgcgtc gcgcaccacg gcgcgcaggt cggacatcaa ctggtcggtc
     5281 gagacggtcg tcatctggct ttcccttggg tgggaggacg attgtatagt catccgatgt
     5341 ccggcctgtc cgaaaggcac tacctcacac cgctgttcga gccggccgcg gtggcgatcg
     5401 tcggcgccac cgaacgcgcg gggtcggtcg gcgccgtgct ggtgcgcaac atgctcgagg
     5461 cggaatacgg cggcgccctg ttcggcgtca atccgaagca caagcaggtg tacggggtcc
     5521 cctgcttcgc cagcgtcgcc aagctgccgc ggcgggcgga cctggcggtg atcgccacgc
     5581 cggcggctac cgtgccggat gtcatcgagc agtgcggcaa ggcaggggtg cgttcggcag
     5641 tggtgatcag cgccgggttc tcggaggccg ggcccgcggg cgtgaagctg gagcgcgcct
     5701 tcctggaaca tgcgcgccgc cacggggtgc gcgttgtcgg ccccaactgc ctcggcatca
     5761 tgcgtcccgc gctcggcctg aacgccagct tcgcgcgcgg cgcggcgctg cccggctcgc
     5821 tcgcgctggt ggcgcaatcc ggcgcggtgt gcaccgcgat gctcgactgg gcgcgcccca
     5881 acaaggtggg attttccagc gtggtgtcgc tcggcggctc caccgacgtg gatttcggcg
     5941 agatcatcga ctacctgatc gaggacgcgc acaccgagca catcctgctg tacatcgagg
     6001 gggtgcgcag cgcgcggcgc ttcgtctcgg cgctgcgcgc tgcggcgcgc gtgaagcccg
     6061 tcatcgtcat gaaggtcggc cgccaggccg ccggctcgcg cgccgcggtg tcgcacaccg
     6121 gcgccatcgt cggcctggac gacgtgttcg acgcggtggt caagcgcacc ggcgtggtgc
     6181 gcgtctccag catcggccag atggtggcgg cggcgcaggc gctcgccgcg catgtgcggc
     6241 cgcagggcga gcggctcgcg gtggtgacca acggcggcgg tcccggcgtg atggccgccg
     6301 accgcgccgc cgacctcggc atcccgctgg ccgagctgtc ggccgccacc atcgcgcgcc
     6361 tgaagcccga gttgccggcc aactggtcgc acggcaatcc gatcgacctg atcggcgacg
     6421 cgggcgccgg gcgctaccgc gccgcggtct ccgcctgcct cgccgacagc ggcgtcgacg
     6481 gcgtgctcgc catcctcgcg ccgcaggcga tgaccgagcc cgagaaatcg gcccaggcgg
     6541 tgatcgaggc ggcgaaaggg tcctccaagc cggtgctcgc ctgctggatg ggcgaggagc
     6601 agaccgccgc ggcgcggcga gtgctcgccg aggcacgcat tccggtgttc cgcacccccg
     6661 acccggcggt ggagatgttc gggcaggtgt cggcgttcta ccgcaaccag cgcgcgctgc
     6721 tgcagacgcc gggaccgctg tcggtgcagg gcgaacccga cctcgatgcg gcgcgcgcca
     6781 tcgtcgccgc ggcgctcaag gagaagcgca gcgtgctctc ggagatggag tccaaggcag
     6841 tgctcgccgc gttccgcatc ccggtggcga agaccgtggc ggcgacctcg gcgcaggaag
     6901 ccgggcgcat cgcgcaggag atcggctttc cggtggcgat gaagatcgac tctcccgaca
     6961 tcacgcacaa gagcgaggtc aacggcgtgc gcctgaacct cggcagcgcg caggcggtgc
     7021 aggccgcctg ggagggaatc gtcgccgacg ccaggcgcct gcgccccggg gcgcgcatcc
     7081 gcggcgtggt gctggagccg atggtgcgtc gcgcccacgg gcgcgagctg atggccggcg
     7141 tgatccgcga cccggtgttc ggcccggcga tcgtattcgg caccggcggc acggcggtgg
     7201 aagtgcaccg cgaccgcgcc gtggcgctgc cgccgctcaa ccagttcctc atcgccgaca
     7261 tgatccgcgg cacgcgcgtg gcgcggctgc tgggcgcatt ccgcaacctg ccgccggtgg
     7321 acatggcgtc gctggaggaa gtgctgctgc gggtctcgga gctggtgtgc gagctgccgg
     7381 aagtggcgga actggacgtc aacccgctgc tcgcggacga agcgggcgtg atcgcggtgg
     7441 acgcgcgcgt ggtgctgcgc ccgggcgcgc cgcggcgcga gcgctacgcg cacatggcga
     7501 tccatcccta ccccgcccgc ctggtgacga cctggcagcc gcgcgagggc ccgctggtga
     7561 cgctgcggcc gatccgcccc gaggacgcgg agatggaaca ggcgttcgtg aaaggcctgt
     7621 cggcgcagag ccgctatttc cgcttcctcg acaccctgcg cgagctcacg cccgcgatgc
     7681 tggtgcgctt cacgcagatc gactacgacc gcgagatggc gttcgtcgcc accgtggcgc
     7741 gcgacggcgg cgaagcggag atcggcgtgt gccgctacgt cgccaacccg gacggcgcga
     7801 gctgcgagtt cgccgtcgtc atcgccgacg actggcagcg gaagggcctc ggccggcgca
     7861 tgctggcgct gctcgtcgac accgcgcggg cgcgcggcct gcgctcgatg atcgggcacg
     7921 tgctggcgga gaaccgcggc atgctgcagc tgtgccagag cctgggcttc gtcgtctccg
     7981 acagcccgga ggggccgatg gtgaggcgcg ccacgctggc gctcgatgcg ggaggcaagt
     8041 agcctatccc catgcgcgaa ggatccctcg aagcccccac ccgccatccg ctcgactggc
     8101 acaacccggc gttcttcgac gaggccgcgc tgcagaagga gcaggagcgc gtgttcgaca
     8161 tctgccacgg ctgccggcgc tgcgtgaacc tgtgcaccgc gttcccgacg ctgttcgacc
     8221 tcgtggacaa ctcgaagacc atggaggtgg acggcgtcgc caaggccgat ttcggcaagg
     8281 tggtggacca gtgctacctc tgcgacatgt gctacatgac caagtgcccc tacgtgccgc
     8341 cgcacccctg gaacgtggac ttcccgcacc tgatgctgcg cgccaaggcc taccagttca
     8401 aggcgggcac gatgaagcac ccggtgcgcg acagccagct ctcctcgacc gatcgcaacg
     8461 gcaagctcgc gacgatcccg gtcgtggtgc agttggtcaa cgccgcggcg aagtcgaagc
     8521 cgctgcgagc gctcggcgag aaggtcgccg gcgtgcacaa ggatgcgtgg gtgcccgagt
     8581 tcacttcgcg ccccttccgg cgcaacgccg ccatgagcaa gccgcatccc gtgaaggacg
     8641 gcgagcgcac gccgggcaag gtcgtcgtct attcgacctg ctacgtgaac tacaacgagc
     8701 ccggcatcgg ccacgacctc ctcaagctcc tcgaccataa cgagatcccg tacgtgatgg
     8761 tcgagaagga ggcgtgctgc ggcatgccca agttcgagca ggg
//
LOCUS       DASRIE010000210         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1524831, whole genome shotgun sequence.
ACCESSION   DASRIE010000210 DASRIE010000000
VERSION     DASRIE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524831"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="gmhB"
                     /locus_tag="VFB53_08890"
     CDS             complement(<1..461)
                     /gene="gmhB"
                     /locus_tag="VFB53_08890"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZN86885.1"
                     /translation="MKLIILDRDGTINEDRDDYVKSPEEWVALPGALEAIARLNHAGW
                     HTVLATNQSGLGRGLFDMAMLNAMHARMNQMLGKHGGRIDAVFFCPHAPEEQCDCRKP
                     LPGLMLQIAERYGVDLSEVPVVGDSLRDLQAGQAAGCPTHLVRTGKCAPLD"
     gene            complement(481..2589)
                     /gene="glyS"
                     /locus_tag="VFB53_08895"
     CDS             complement(481..2589)
                     /gene="glyS"
                     /locus_tag="VFB53_08895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZN86886.1"
                     /translation="MNAVKKNLLVELFVEELPPKALKRLGEAFAGALAEGLRGQGLAG
                     ATSVVTPFASPRRLAVHVTDVAASAADRAVSQKLMPVSVGLNSEGQPTPALLKKLGSL
                     GVEASAFPQLKRQPDGKAEALFLDSVVKGATLAGGLQKALDEAIAGLPIPKVMQYQLD
                     DGWTSVNFVRPAHGLVALHGAEVVPVAALGLQAGRTTQGHRFEARAPIVNLRDADGYA
                     AQLREEGAVVASFAERRAEIVRQLGVAAREQGLAPIEDEALLDEVTALVERPHVLLCR
                     FEPEFLQVPQECLILTMKANQKYFPLLDPAGRLTDRFLVVSNIRPDDPSAVVGGNERV
                     VRPRLADAKFFFDQDRKRTLASRVPGLAKVVYHNKLGTQGERVERVRAIARAIAKTLG
                     GDPLVQQADQAAMLAKADLLTDMVGEFPELQGVMGAYYARHDGLGDDIAFAVEDHYKP
                     RFAGDALPRNLAGIAVALADKLETLAGLFGIGQVPTGDKDPFALRRHALGLIRMLVER
                     ALPLDVTELVSVAFHAFPPGHGQAQGEVGFFLRERLTGYLRDAGYSAQEVEAVVEAKP
                     RLWADIPKRLAAVRAFKALPEAEALAAANKRVGNILRKSEQGAAGSVDRARLTEPAEQ
                     ALFEALQRVVPRSDDAFACGDYTASLQALAALKTPVDAFFDDVMVNVDDVGLRANRLA
                     LLSQMHAAMNRVADLSRLAS"
     gene            complement(2594..3511)
                     /gene="glyQ"
                     /locus_tag="VFB53_08900"
     CDS             complement(2594..3511)
                     /gene="glyQ"
                     /locus_tag="VFB53_08900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZN86887.1"
                     /translation="MLTFQQIILKLQSYWDAQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGQNPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     DLRSNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITY
                     GLERLAMYLQGVDNVFDLQWTEGLKYGDVYHQNEVEQSTYNFEHSDVEFLLGAFGAHE
                     RQAKHLMEHQLALPAYEQVLKAAHAFNLLDARGAISVTERAGYIGRIRNLARSVAQSY
                     LESRARLGFPMAPRAWAHDVLAQLEKKAA"
BASE COUNT          488 a         1258 c         1262 g          547 t
ORIGIN      
        1 tcgtccagcg gcgcgcactt gcccgtgcgc acgaggtgcg tcgggcagcc cgccgcctgg
       61 ccggcctgca ggtcgcgcag cgagtcgccc accaccggca cctccgacag gtcgacgccg
      121 tagcgctcgg cgatctgcag catcagcccg ggcagcggct tgcggcagtc gcactgctcc
      181 tcgggcgcgt gcgggcagaa gaacaccgcg tcgatgcggc cgccgtgctt gccgagcatc
      241 tggttcatgc gcgcgtgcat ggcgttgagc atcgccatgt cgaacaggcc gcgaccgagc
      301 cccgactggt tggtggcgag caccgtgtgc cagccggcgt ggttgaggcg cgcgatggct
      361 tccagcgcgc ccggcagcgc cacccactcc tcgggcgact tgacgtagtc gtcgcgatcc
      421 tcgttgatcg tgccgtcacg atcgaggatg atgagcttca tcggggcggt cggcacgacg
      481 tcagctcgcc agcctggaaa gatcggccac gcggttcatc gccgcgtgca tctgcgacag
      541 cagcgccagc cggttggcgc gcagcccgac gtcgtccacg ttgaccatca cgtcgtcgaa
      601 gaacgcatcg accggcgtct tgagcgcggc cagcgcctgc agcgacgcgg tgtagtcgcc
      661 gcaggcgaag gcgtcgtcgg agcgcggcac cacgcgctgc agcgcctcga acagcgcctg
      721 ctcggccggt tcggtgagcc gcgcgcggtc caccgatccg gcggcgccct gctcgctctt
      781 cctgaggatg ttgcccacgc gcttgttggc ggcggccagc gcctcggcct cgggcagcgc
      841 cttgaaggcg cgcaccgcgg ccagccgctt cgggatgtcg gcccacaggc ggggcttcgc
      901 ctcgaccacc gcctccactt cctgcgcgct gtagccggcg tcgcgcaggt agccggtcag
      961 gcgctcgcgc aggaagaagc ccacctcgcc ttgcgcctgg ccatggcccg gcgggaaggc
     1021 gtggaaggcg accgacacga gctccgtcac gtccagcggc agcgcgcgct cgacgagcat
     1081 gcggatgagg ccgagcgcgt ggcggcgcag ggcgaacggg tccttgtcgc cggtggggac
     1141 ctgcccgatg ccgaacaggc ccgcgagcgt ttccagcttg tcggccagcg ccaccgcgat
     1201 gccggcgagg ttgcgcggca acgcgtcgcc ggcgaagcgc ggcttgtaat ggtcctcgac
     1261 ggcgaaggcg atgtcgtcgc ccaggccgtc gtggcgcgcg tagtacgccc ccatgacgcc
     1321 ttgcagctcg gggaactcgc cgaccatgtc ggtgagcagg tccgccttgg cgagcatcgc
     1381 cgcctggtcg gcctgctgca cgagcggatc tcccccgagc gtcttcgcga tggcgcgcgc
     1441 aatggcgcgc acgcgctcca cccgctcgcc ctgggtgccg agcttgttgt ggtagaccac
     1501 cttggcgagc ccgggcacgc gcgaggccag cgtgcgcttg cggtcctggt cgaagaagaa
     1561 cttcgcgtcg gccaggcgcg ggcgcacgac gcgctcgttg ccgccgacca cggcgctcgg
     1621 gtcgtccggg cggatgttgc tcacgacgag gaagcgatcg gtgagccggc ccgccggatc
     1681 gagcagcgga aagtacttct ggttggcctt catcgtgagg atgaggcact cctgcggcac
     1741 ctgcaggaac tcgggctcga agcggcacag cagcacgtgg gggcgctcca ccagcgccgt
     1801 cacctcgtcg agcagcgcct cgtcctcgat gggcgcgagc ccctgctcgc gcgccgcgac
     1861 gcccagctgg cgcacgatct ccgcacggcg ctcggcgaag gaggcgacca ccgcaccctc
     1921 ctcgcgcagc tgtgccgcgt agccgtcggc gtcgcgcagg ttgacgatgg gcgcgcgcgc
     1981 ctcgaagcgg tggccctgcg tggtgcggcc ggcctgcaag ccgagcgcgg ccaccggcac
     2041 cacctcggcg ccgtgcagcg ccaccaggcc gtgggccggg cgcacgaagt tgacgctggt
     2101 ccagccgtcg tcgagctggt actgcatcac cttgggaatc ggcaggcccg cgatcgcctc
     2161 gtcgagcgcc ttctgcaggc cgccggcgag cgtcgcgccc ttcaccacgc tgtcgaggaa
     2221 gagcgcctcg gccttgccgt cgggctgccg cttgagctgc gggaacgcgg acgcctcgac
     2281 gccgagcgag cccagcttct tcagcagcgc cggcgtgggc tgcccctcgc tgttcaggcc
     2341 caccgagacc ggcatcagct tctgcgacac cgcgcggtcg gcggcggacg cggcgacgtc
     2401 ggtcacgtgc accgccaagc ggcgcggcga ggcgaacggc gtgaccacgc tcgtggcgcc
     2461 cgccagtccc tggccgcgca ggccctccgc gagcgcgccg gcgaaggcct cgcccagcct
     2521 cttcagcgcc ttgggcggca gctcctcgac gaacagctcg accagcaggt tcttcttcac
     2581 ggcattcatg cgttcaggcc gccttcttct cgagctgcgc gagcacgtcg tgcgcccagg
     2641 ccctcggcgc catcgggaag cccaggcgcg cgcggctctc gaggtagctc tgcgcgacgc
     2701 tgcgggcgag gttgcggatg cggccgatgt agccggcgcg ctcggtgacg ctgatcgcgc
     2761 cgcgcgcgtc gagcaggttg aaggcgtgcg cggccttgag cacctgctcg taggccggca
     2821 acgcgagctg gtgctccatc aggtgcttcg cctggcgctc gtgcgcgccg aaggcgccga
     2881 gcaggaactc cacgtcgctg tgctcgaagt tgtaggtgct ctgctccacc tcgttctggt
     2941 ggtacacgtc gccgtacttc agcccctcgg tccattgcag gtcgaacacg ttgtccacgc
     3001 cttgcaggta catcgcgagc cgctcgaggc cgtacgtgat ctcgccggtg atgggcttgc
     3061 agtcgatgcc gcccacctgc tggaagtagg tgaactgcgt cacctccatg ccgttcagcc
     3121 acacctccca gccgaggccc caggcgccga gcgtggggtt ctcccagtcg tcctcgacga
     3181 agcggatgtc gttggagcgg aggtcgaagc ccagcgcctc cagcgagccg aggtacagct
     3241 ccaggatgtt ggccggcgcc ggcttcagca ccacctggta ctggtagtag tgctgcaggc
     3301 ggttgggatt ctggccgtag cggccgtcct tcgggcggcg gctcggctgc acgtacgccg
     3361 ccttccacgg ctcggggccg agcgcgcgca ggaaggtggc ggtatggctg gttccggcgc
     3421 ccacctccat gtcgtagggc tgcagcagcg cgcagccctg cgcgtcccag taggactgca
     3481 gtttcaggat gatttgctgg aaggtcaaca tcggggaagc gcaggggaaa accgcagatt
     3541 ttacggtgcg ggtcg
//
LOCUS       DASRIE010000211        36329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3883966, whole genome shotgun sequence.
ACCESSION   DASRIE010000211 DASRIE010000000
VERSION     DASRIE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 36329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3883966"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFB53_08905"
     CDS             <1..656
                     /locus_tag="VFB53_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HZN86888.1"
                     /translation="GTGSFRDYLDQVETASSGEREQFVNALTTNLTAFFREPHHFEML
                     AAKARGAGRLRVWSSACSTGEEPYSAAVALREAGCAAEILATDIDTETLATARNGVYR
                     IDALEAVGIERMRPHFLRGTHENARFAKVRPELRGMVKFDYRNLLSQDWPPRERFDAV
                     FCRNVMIYFERTAQRSLLERFAAVLAPGGLLFLGHSENCATGHPKFRACGKTTYLRQ"
     gene            653..1249
                     /gene="cheD"
                     /locus_tag="VFB53_08910"
     CDS             653..1249
                     /gene="cheD"
                     /locus_tag="VFB53_08910"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HZN86889.1"
                     /translation="MNETVALYFDRQFDREAVKILPGQYYAADRDIVIVTVLGSCVSA
                     CLWDPVKRIGGMNHFMLPGAGPAAGAAGAGARMGIYAMELLINRMLKLGAERGRLVAK
                     VFGGASVLEGMDALNIGSQNSAFVLDFLRVEGIALAAQDLYDVCPRKVYHFPHTGKVL
                     VKKLGALRNDTLQRRERDYLATLKDSAGAGEVEIFGCR"
     gene            1249..2301
                     /locus_tag="VFB53_08915"
     CDS             1249..2301
                     /locus_tag="VFB53_08915"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HZN86890.1"
                     /translation="MDRIRVLVVDDSAVMRGIMSRIINAQPDMTVAAVASDPLVAMER
                     LRTAPPDVIALDVEMPRMDGLQFLARLMQWRPTPVVMVSSLTAQGAETTLRALELGAV
                     DFVAKPLLAGGREFEDYAQEVAEKLRAAAGARVGRRPGGAAAAGTPKPRQGAAGGGSR
                     DQVIVIGASTGGVEALREVLVPLPCGMPPILVAQHMPAGFTRSFAQRLDALCQVEVKE
                     GEDGEPLRRGHAYIAPGGRHLELCARAGKLEARLTQEPPVNRHRPSVDTLFRSAAQAA
                     AARCIGVMLSGMGADGADAMLELRRRGARNIAQDEASCVVYGMPKQAVAAGAVHELLP
                     LREIPSRLIELCLANG"
     gene            2391..2849
                     /locus_tag="VFB53_08920"
     CDS             2391..2849
                     /locus_tag="VFB53_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HZN86891.1"
                     /translation="MRNKLIYALLAAAFAGPAAAGDTATLAVSAVVAGTCKFTTATFN
                     MSFGTLDPSAAANQTQSAGITYKCTKNAAATSFSFDGDAASPASVNITNGIDNIPVAL
                     SWTAPATLGSGFGAGSSAITMNVTGDILGTDYADVSAGNYTRNVAVVVAP"
     gene            complement(2860..3630)
                     /locus_tag="VFB53_08925"
     CDS             complement(2860..3630)
                     /locus_tag="VFB53_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN86892.1"
                     /translation="MPRIAVLEDDPSQAKLLQHWITSQGFVCRLFERGNDILRVLLRD
                     TFDLVILDWRVPDLSGEEVLKAIRKNMREPLPVLFTTGRDREEDIVHALKSGADDYLV
                     KPLRRMEFLARVDALLRRARGAGRDAEEPIEAGAYRIDPHGRTLHKNGQVIEMTQKEF
                     EMAVLLFRNVGRLLSRAYLLDTVWGISADVTTRTVDTHASQLRSKLGLYPEEGWRLSA
                     VYQHGYRLERSAPPSPAAGEAPAPGKPEPAGRPRRKRA"
     gene            3725..4114
                     /locus_tag="VFB53_08930"
     CDS             3725..4114
                     /locus_tag="VFB53_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86893.1"
                     /translation="MTGPRTSILHVEDDPSLQNLVRITLEQLGGYAVRTAANGYQALD
                     LARQALPDLLLLDLDLPGMNGIATLRALREIDGMGEVPVVFLTAASDLMGDAEFRAFG
                     VREVLPKPFRPRQLVQALARVLGPRGD"
     gene            4117..5625
                     /locus_tag="VFB53_08935"
     CDS             4117..5625
                     /locus_tag="VFB53_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN86894.1"
                     /translation="MQPSSYGTHATPDDTGGLARLVADNAPSTLVYLDADLRIRFANR
                     HCYELFGRTPREVLGRPLADLLDPRTLRYALAHRAEVERGNLAPRDYVLSDREGDRKF
                     VKVHAVPNRDRSGRSVGYFACTSDNAAERVTRAALSAAEERLSFALAACEAGIWVWDL
                     AAHTAHYTAEFKALLGYGEGEFPPDFGFFGALHAEDGAAMFDAVAAAIQDGGVFDREF
                     RIRCADGGYRWLRCIGRAIVDPATGSTVRLAGTVRDISPRKQVESRLREAQALVQATL
                     EGQLAAAEQQGERERLDQVKRELVAAAGHELRTPLASIIAALELLCEGKALGTERAAE
                     SFLRLALRNAERLARVVEQWLDVERIDLGVTRVQAVALDLGALVARVIAERAAAAQRH
                     SLRFETGGGLGSVLIRGDAERLRQAVAHLISGAIDRSPPGAVVSVRIEVHGDKATLHV
                     EDEGPELFSGADLGLSLSKAIVERLGGTLGCTGRQGKGAAYQLALPRLAAAN"
     gene            5649..6059
                     /locus_tag="VFB53_08940"
     CDS             5649..6059
                     /locus_tag="VFB53_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZN86895.1"
                     /translation="MRNEALLDLETIRDLAEFDGGPGAPFAVELLEMFTADAHRALLR
                     MRVSAWLGDAGALAREAHRLKGSSGSVGATRLQAECLALERCAREGNLGELVGLEERI
                     EHALDVLQATREGILQCDALFRKAERCSTMERAI"
     gene            6160..9234
                     /locus_tag="VFB53_08945"
     CDS             6160..9234
                     /locus_tag="VFB53_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN86896.1"
                     /translation="MRESSAQFRLAQEALGFVTWIWDVRDDRVQWYGDMSRLLGLAPG
                     SFSGRFEDYLTFVHPEDVAGARATFLDCLKGKRPEYRAVERVVWPDGSVHWLETYGRA
                     EYGADGRAVRAAGAIKDISERKRMESARLRAEQQLARVFDASPDYIAIVRASDGRFVA
                     ANRAFEQVTGYRADEVVGRTVAEIGIWAIPGERERFLADLHKGVAVRDRPMLLRVQGG
                     RIVSGTISASLIEHDGEQLIIGMVHDITEAKRLERRARQSEHKFAALFENSPEAITLY
                     RLSDGMRLEANSAWERTTGYSRAQAKGRPAKEMGLWKDQAQRAAVIARIDTEGSVSNV
                     EARLVRADGSELDALISAVRIDLEGEACVLWCWRDVSAERAVERRAQQSERKFAAMFE
                     TSPVGLVVTRPRDRRIVEINDAALQLIGLGRDEAIGAMTTDVVEILDQEQFQRIRSRG
                     LAGERVAGEPLRFRRRDGKQVEALISGATVEFDGEPHFVVSLLDVTEQRRVERERQQA
                     DARYRALFESTSDSIAIFSPDWKFIDLNAGNRRLSGYAREELIGQPVTMVLDQEDLAQ
                     KPIRDDRDWTLIERTLRRKDGSKRAIEVMSGRLPDGNIIVIARDISERKRSETLLMNV
                     ARGVSAELGEAFFRSLVEHLARELDADFAFIAEVVQPDNERCRTLAYVADGAIAPNFE
                     YPLAGSPCLNAITQRGTVMYPQGVAQQFPLDAGLGRNGIEGYVGTSLHGADGTALGVL
                     VVMHRKPIERSQFWASMIEIFGARAAAEIERSRAEALVRRTNASLEQVVHERTSQLEE
                     ANRDLESYNYSISHDLRQPLNAIAGFAELVRDTAGSALQSGTREFVREIESNAERMER
                     MIEALLQLSRAGRGPLDKAEVQMGPLVEAVLRDLGVGAPLAAQIEVGELPAAQGDEVL
                     LRQVWANLIGNALKYSRLSRAPRIRISGARRDGFVEYEISDNGVGFDMLHAERLCETF
                     HRLPSAVSFEGNGVGLAIVQRVLRRHGGSIRAESAPGAGATFRFTLPDEALA"
     gene            9297..9683
                     /locus_tag="VFB53_08950"
     CDS             9297..9683
                     /locus_tag="VFB53_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86897.1"
                     /translation="MLLATGFLAAAPQLPAHAAEPSISLYCARGVTDYPDAELTLSTR
                     GSHLVKVVFVGYRPSRARADWSLRDCLSTAIKFDGSRDIVASLWYRDRALRSPPEPLR
                     PDGAAASLVYQAARHAVVVQRRETRP"
     gene            9758..10486
                     /locus_tag="VFB53_08955"
     CDS             9758..10486
                     /locus_tag="VFB53_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HZN86898.1"
                     /translation="MSRRLLAAVFWLATTCAGAAEFSVSPIRIELPRGARSAAVSVAN
                     EDRQPLRMQLRLMEWTQDSDGKDLYRESDALIYYPRLMTIQPREKRLVRIGVKAASAA
                     AERTYRLYIDELPGVADDPLRPAVSGINFTLRFALPVFVPPAEPALRGVIEAMSLQDG
                     KLAVTVRNAGNQSFRIASVAARGGAFAAEAGGWYLLAGAARVHRFDIPAEACRELRRI
                     EVAVTADRLSLEGGLDVDPGMCPR"
     gene            10458..12836
                     /locus_tag="VFB53_08960"
     CDS             10458..12836
                     /locus_tag="VFB53_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HZN86899.1"
                     /translation="MSIRACVLAELAAAALGACGLAAAAEEPLRLKLAPALSQARPAL
                     EPLIVEVSVNQHRRGEFTVYRDADGDYYLRASELGALGLAEHAAAPRTVQIDGEAFVS
                     LRAFAPRRLFLDESRLALEIEFPAPALRKSTYDLMPRRPDLPQGPREPGFLNYRLSAS
                     DGSGAERRRFGLAHELAVRAGDVLLRHEAALLHGAGGTRGLRYATQIVHDRPAEQQRL
                     VLGDHVAASGELGSTLAVGGISFAKHYPLAPHFIRQPLAGYAGAVSTPSQVEVRMGGV
                     PVFREQVPAGPFELRNLQQFAGARDVEIVMRDAMGREQTIGFPHYFADQALRRGLHEY
                     SYSAGALREALGVRSNQYGAGVLTAVHRYGLSDRATLGLRAEAAEGLGNVGPTALYRD
                     DRLGVFSGALALSRREGRSGVATALAHAYQTPRFGLRSSARRYSADYAVAQDLVAPAW
                     LQAEYGLGGSLLLGRFGSVFLDRSIVKRNASVSLHDSATTQLSYTYSLGRQGSLFATL
                     ARLHAERTDTRAFVGVLIALDRAKTAHLSARREPDGDAALYGAQLASTVPAGEGLGYR
                     VGVDGIGGSSKQWSGFAQYNARAASLTLDATSTRAAGEGAGRYEIAVAGAATWAGGRL
                     GLARQVEDSFVAVQLAAPLEGVRVYSNNQEVGRTDRHGRLIVPNVGSFHETQIAIDES
                     DVPLDHAIGAMRLVVAPAYRSGSLLRFDVRRQRAIEGVLLVRSREGLRPAENALVRVG
                     DREFTTGRDGRYYVEDLPPGRHQARLGACAFQLVVPDSAEPVATLPEAVACE"
     gene            12829..13302
                     /locus_tag="VFB53_08965"
     CDS             12829..13302
                     /locus_tag="VFB53_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HZN86900.1"
                     /translation="MSRFLCAALLILAPSLACAQVACGFLSTPGAAFGVYDDGAPGAA
                     DTATSVVVRCLRNGGPANVTLTLQIGPSAGSGQIASRALSAGAERLVYNLYRDASRSA
                     VWGQTAGVDTGAVALNGIPNNGSKDATFVVYGRIPALQSVAAGAYADSVQLTISP"
     gene            13316..13714
                     /locus_tag="VFB53_08970"
     CDS             13316..13714
                     /locus_tag="VFB53_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86901.1"
                     /translation="MLARLILCALICPAPAAQAADLGITAVVARSARVTLLDQPSRVI
                     VTPEDVARGYVDLPSGPRLGVRSNSREGIAVAVVSTHPLLGIELRGGHTPLVLPGGVR
                     SLDLHYRVLLAPDALPGIYAWPGQIIATPL"
     gene            complement(13695..13895)
                     /locus_tag="VFB53_08975"
     CDS             complement(13695..13895)
                     /locus_tag="VFB53_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZN86902.1"
                     /translation="MQPVKTVPCPRCGAAAAFSHDNKWRPFCSERCRLIDLGKWASED
                     YRVPTKEEAARQPPEDPTEESR"
     gene            complement(13883..14641)
                     /gene="zapD"
                     /locus_tag="VFB53_08980"
     CDS             complement(13883..14641)
                     /gene="zapD"
                     /locus_tag="VFB53_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HZN86903.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARTDPLDHHVALLTL
                     FEILEVSSRADLKSDLLQELERQKQVLLSFRNNPEISEEALSSVLRDIEQAAAALFSM
                     AGRIGQYIRENDWLMAIKQRTGIPGGACEFDLPSYHYWLHRPAEERIGQLAAWTGPLF
                     PLRDGSAIILRILRESGKPQKLAAPLGTFQQMLGGKSAQMMRVRLDPAANCVPEISAN
                     KYVLNIRFLTQASEDPKPRAADWDVAFELTFCNL"
     gene            complement(14703..15311)
                     /gene="coaE"
                     /locus_tag="VFB53_08985"
     CDS             complement(14703..15311)
                     /gene="coaE"
                     /locus_tag="VFB53_08985"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903899.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZN86904.1"
                     /translation="MAYVVGLTGGIGSGKSAAAEEFGRLGATLVDTDAIAHELTQAGG
                     AAMPLIRRQFGEAYVDAAGAMDRARMRELAFRDGAARKALEALLHPMIRAESERRIAA
                     AAGPYVIHVVPLLVESAGYRQRVARVLVVDCPPEVQLARVRERSGLSADEVGRIIGAQ
                     IKREVRLAAADDVIDNSGTLDALHKQVRELHAHYLQRARAAP"
     gene            complement(15311..16171)
                     /locus_tag="VFB53_08990"
     CDS             complement(15311..16171)
                     /locus_tag="VFB53_08990"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN86905.1"
                     /translation="MAVSGWLVQPAVLPWMALILGLCVGSFLNVVVHRLPKMMEREWR
                     EQCADLAGTALPPAGRYNLVVPRSACPACGARIKALHNVPVFSWLALRGRCAGCGKRI
                     SARYPAIEILAGLIGAYAAWRFGPTLAAAGAMGFGWALLALTAIDLDTQLLPDDITLP
                     LLWAGLLINMTGAFVPLRTALIGAVAGYLALWLVYWGFKLATGKEGMGYGDFKLLAAI
                     GAWLGWQKLPLVILLSSVVGAAVGIGLMVLARHGRDKPIPFGPYLAAAGLIALFWGED
                     IVRRWLPILG"
     gene            complement(16172..17398)
                     /locus_tag="VFB53_08995"
     CDS             complement(16172..17398)
                     /locus_tag="VFB53_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN86906.1"
                     /translation="MATAAAAKAGGPKEFNFVWEGRDKGGKVIRGELRSVSETAVSAS
                     LRRQGIMVQKVKKQKYGSGGKVKEKDITLFTRQLATMMKAGVPLLQCFDIVGKGASNP
                     AVARLLQEIKTEVETGSSLAQAFRKYPLYFDALFCNLVAAGEQAGILETLLDRLASYK
                     EKILAIKSKIKSALFYPIAIIVVAFIITAVIMIFVIPAFKEVFSNFGADLPAPTLMVM
                     AISDWFVANWYIIFPVIIGAVIGFLEAWKRMLPVQIFMDRLMLKLPVFGDLVLKSTIA
                     RWTRTLSTMFAAGVPLVEALDSVGGASGNNVYLTGTKKIQQEVSTGTSLTMAMTNSGL
                     FPNMVLQMCSIGEETGALDSMLSKVADFYEAEVDDAVEALSSLMEPMIMVVLGTLIGG
                     MVIAMYLPIFKMGQAV"
     gene            complement(17560..18879)
                     /locus_tag="VFB53_09000"
     CDS             complement(17560..18879)
                     /locus_tag="VFB53_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZN86907.1"
                     /translation="MEILILACLILLNGLFAMSEVAVLTSRRPRLAALASGGDRLAAA
                     ALRLVEAPTRFLSTVQIGITSIGILNGIIGEAVLAAPVAVWLQSLGLEHKTSAIAATA
                     VVVMAVTYFSIVVGELVPKRLGQHNSEAIARLVAWPMRLLAGASAPFVNLLSLSTEAI
                     LKPLLVLLRIRPMESGAQPLSPEEIRALVLESSSFMPKKHVSILLNLFDLGAITVQDI
                     MLPRARIEAIRLDDDMEAIARHLATSYHMRLPVFRDSRGEVLGILHLRKVLSPLQAGT
                     LNKAEIESLLVPPYFVPATTPVFAQMQYFQENEERIAMVVDEYGELMGLVTLEDIIEE
                     IIGKFTTSMPSASQALAWSEDGTATADGATPVREVNRALGLNLPTEGPKTLNGLILEQ
                     LRDIPEADVSIKVGEVAMEIVHAQGRTIRTVRLFRPQPQLHSQQELP"
     gene            complement(18889..19767)
                     /gene="ccsA"
                     /locus_tag="VFB53_09005"
     CDS             complement(18889..19767)
                     /gene="ccsA"
                     /locus_tag="VFB53_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZN86908.1"
                     /translation="MPDIVIHALAAAGYAGLAWHFWNSRWRARSAGGAAPGGGLRPWE
                     RAAILAALALHGWLLHAVLFAGALRFGFAHALSAMLWLAVLFYWAESLFYNLEGMQAP
                     ALALAALAVPLPALFPGLASGAYADSLEFRLHLVLAMLAYSLFTIAILHALLMAVVER
                     RLHQTRQARGAGGALLADGVAMLQGPLGALPPLLTLERLLFRLIAAAFVLLTCTLATG
                     FAFADALFGRPLRFDHKTLFALLSWATFALLLAGRHFRGWRGRTALRWTLSGFAMLLL
                     AYVGSRFVMEVLLGRS"
     gene            19786..21135
                     /gene="ffh"
                     /locus_tag="VFB53_09010"
     CDS             19786..21135
                     /gene="ffh"
                     /locus_tag="VFB53_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZN86909.1"
                     /translation="MLDNLTARLAKIVKTMRGEARLTEGNVQEALREVRVALLEADVA
                     LPVVRDFVAAVKQKALGAEVVGSLTPGQALVGVVHRELARLMGETSVGLDLAAQPPAV
                     ILLAGLQGSGKTTTAGKLARLLRAQKKKVLLVSCDVVRPAAIEQLAAVAAQAGADVFP
                     SAAGEQPADIGRRALEHARTRYYDILLVDTAGRLAVDEAMMQEIAALHAALRPVETLF
                     VVDAMQGQDAVNVARAFGERLPLTGVILTKLDGDARGGAALSVRQVTGRPIKFAGVGE
                     KLDALEPFHPERMAARVLGMGDILSLVEEAHKQVDLKEAQRVAEKLKKGKGFDLEDFK
                     QQLLQMQKMGGMAAMLDKLPAQLAGQINPGQLQDERQLRRTAGVINAMTPQERAHPEL
                     IKASRKRRIAAGAGVPVQEVNRLLGQFEQMQKMMKMMKGGNLARMMRGLKGALPGMR"
     gene            complement(21137..22102)
                     /gene="corA"
                     /locus_tag="VFB53_09015"
     CDS             complement(21137..22102)
                     /gene="corA"
                     /locus_tag="VFB53_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZN86910.1"
                     /translation="MLVNCVAYQDGRKLAEITRREIKSYLSRPDCFVWVALKDADAAE
                     LAEMQDEFDLHELAVEDARHGHQRPKIEEYGDSLFAVLHTVEAAGEELKVGEMDVFVG
                     RNYVLSVRQHSERGFAEVRARCEREPELLRHGSGYVLYALMDAIVDRYFPVLDLLEVE
                     LEALEEKIFSGKSPRENIEALYYVKQKLMTLKHAVAPLVEASARLYGGRVPQVCAGLG
                     EYFRDVYDHLFRLNQSIDTLRDTVTTAIQVNLAMITIGESENTKRLAAYAALVAVPTM
                     IAGVYGMNFENMPELKWSLGYPVTIAVMVALDSYLFYRFRKSGWL"
     gene            22150..23364
                     /locus_tag="VFB53_09020"
     CDS             22150..23364
                     /locus_tag="VFB53_09020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN86911.1"
                     /translation="MPAISRRAQGLGTENAFVVLAEVNALARQGKDIVSFCIGQPDFP
                     APENVQEAAIRAIKGGKHGYTPSAGIDELRAAAARYMAGMRGGLPIRAEDVVVGAGAK
                     PFIAYAILSTTDYGAGDEVIYPNPGFPIYESQIAANGAVPVPIHLHEARDFAFDPAEL
                     ERLITPKTKLLILNTPHNPTGGMLARKDLEAIAAILRKHPQVWVYADEIYSRLCYAGE
                     FLSIAQLPGMYERSIISDGASKTWAMTGWRIGFTSNPVLAPHFTRWITNTESCASQIS
                     QWAALEAINGPQDAAAMMRASFLERRDLIVELLGKVPGVACRNPGGAFYAWPNVTDAC
                     RMIGAADSEEFRKRLLHEAGVAVLADIHFGSRVPGEGQHLRFSYAASKAQIEAGVARM
                     AEFIRKNTRKAA"
     gene            23408..23863
                     /locus_tag="VFB53_09025"
     CDS             23408..23863
                     /locus_tag="VFB53_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN86912.1"
                     /translation="MTRWRPSVTVAAVIERGGKFLLVEEEASGRRVLNQPAGHLDPGE
                     SLVQAVVRETLEETAHRITPTALLGIYRWRYDKEEDVTFLRFAFLGRVEGVEAGRQLD
                     REIIAALWLTPAELAARRAEHRSPLVQQCVDDCLAGRSFPLDALSRAYA"
     gene            23856..24914
                     /gene="mnmA"
                     /locus_tag="VFB53_09030"
     CDS             23856..24914
                     /gene="mnmA"
                     /locus_tag="VFB53_09030"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZN86913.1"
                     /translation="MPEKVVVGMSGGVDSSVAALLLKRAGHEVVGLFMKNWEDDDDDA
                     YCSTRQDFLDAAAAAEVIGVELEAVNFAAEYRERVFASFLAEYSAGRTPNPDVLCNAE
                     IKFKAFLDHAMRLGAARIATGHYAQLRQAAGKVELLRGADAGKDQSYFLHRLTQAQLS
                     RVLFPIGHLKKTEVRRIAAAAGLPNHAKKDSTGICFIGERPFREFLNRYLPKTPGAMV
                     HPDGKRVGEHIGLAFYTIGQRKGIGLGGAGEPWYVAEKRMATNELVVVQGHDHPLLLR
                     RSLQGLDASWVLAAPAAGAAHAAKTRYRQADSACTVTRVGQGALAVEFPQPQRAVTPG
                     QSVVLYDGEVCLGGAVIA"
     gene            complement(24911..25528)
                     /locus_tag="VFB53_09035"
     CDS             complement(24911..25528)
                     /locus_tag="VFB53_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZN86914.1"
                     /translation="MTMKLLGAPASPYTRKVRIVLAEKRIECDFERVDVQPADNPVNQ
                     HNPLGKIPTLVLDDGTALYDSRVIVEFLDNASPISRLIPEENRERLAVRRWEALADGV
                     LDAGLLVRYESLRPQPEQSAAWAGKQAARIGRGLAQMQADLGDRPWCQGERYTLADIA
                     LGCCVGWIGFRKPAGIEWRTQYPGLAKHYDKLMERAAFADSVPAV"
     gene            complement(25540..26109)
                     /locus_tag="VFB53_09040"
     CDS             complement(25540..26109)
                     /locus_tag="VFB53_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZN86915.1"
                     /translation="MLRNLIKEARACRVCAPHLPLGPRPVFLVGARARLMIVGQAPGR
                     RVHETGIPWNDASGERLRDWLRLDRAQFYDTRRVAIVPTGLCYPGSGERGDLPPRPEC
                     APLWQPRFRAALPGIRLTLLIGMHAQAYFLGRRRKPTLALTVRAWREYLPEYFPLPHP
                     SPRNRLWLERHPWFVKEILPALRRRLRAL"
     gene            26130..27494
                     /gene="purB"
                     /locus_tag="VFB53_09045"
     CDS             26130..27494
                     /gene="purB"
                     /locus_tag="VFB53_09045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZN86916.1"
                     /translation="MPSPLGALSPLDGRYEKSADPLRPYFSEAALIRHRVLVELEWLK
                     ALAAERHIRELPAFSRRTVAALDRLVAGFGDQDATHIKNIEAETNHDVKAIEYWLKGR
                     LAKNAEVQRSLEFIHFACTSEDINNLSYALMLQHARDRVMLPRLDAVIAALRAFARRH
                     AARPMLSRTHGQPASPTTLGKEMANFAARLQRAREAIARVAILGKINGAVGNYNAHAA
                     AYPDFPWEAFCRRFVERLGLEFNPYTTQIEPHDSYAELFDAYARANTVLLGLDRDLWG
                     YVSLGYFRQKAKAGEVGSSTMPHKVNPIDFENSEGNLGVANALLRHLADKLPVSRWQR
                     DLSDSTALRNAGTALGHTLLALQSCLRGLAKLEADPARMAEDLDANWEVLAEAVQQVM
                     RRHGVPGAYEKLKALTRGKRLDRRQLAAFVKALPIPAEAKKRLLALTPASYTGLASAL
                     AKRI"
     gene            27491..28273
                     /locus_tag="VFB53_09050"
     CDS             27491..28273
                     /locus_tag="VFB53_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86917.1"
                     /translation="MRLLLAALLACAAPLHAQELVTLPTRPGVTLSFFIANMGKVQPR
                     AAALMLIGGGGNIRLRMEGGKVHFGQQNFLPRTRREFIAQGILPVILDNPTDQQAGDG
                     MTDDFRAGAKHTEDLRAVLAEVKRRHPGLPVFLVGTSRSTLSAAYQARALGDALAGAV
                     LSSSMFRTREVPVLAVFDFASIRIPFLFVHHRADACHATPYYDAERLGRKYPLVSVSG
                     GKPPETGPCDPLSAHGYFGKEAETVAAIAAWMLGRPFDKEIN"
     gene            28281..30287
                     /locus_tag="VFB53_09055"
     CDS             28281..30287
                     /locus_tag="VFB53_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZN86918.1"
                     /translation="MLHAAVIALSSFLLFLVQPLIARLILPWFGGGAAVWTTCMLFFQ
                     SLLLAGYAYAHASSARLAPRPQAMLHIALLAAAAATLPIAPSERWQPEGHEEPVTAIL
                     LLLGAAVGLPYLLLSATSPLVQAWFARARPGANPYRLFALSNVASLAALVGYPLAVEP
                     LLSNGEQVAGWSWLFALFALLCAALAWRSAAPAAPGAAVLAEAPAPGRRDLLHWLALS
                     ATGSVVLLAVTNHVTQNIAAIPLLWLAPLTLYLLSFILAFEGRLYRSEMFWAPCLAAL
                     LGMAWLLVDADFQFDLPVQLGVFLAGLFVTCLFCHGELYRLRPAPRHLTAFYLTVSAG
                     GALGGLLVAVVAPLAFNGYYELGVAMIAVAFLAALRCASLHRVARFASLAVLMAVSGA
                     AIYDGLRFAQDVRLASRNFYGVLRVKEYGTPGDTGQLRRLMHGVIMHGEQYQHADRRR
                     QATSYYQHSSGIGAALLARQARPGPMRVGVIGLGIGTLAAYGRQGDTYRFYEINPRVM
                     QVAVGAFTYLKDSEARIEMKLGDARLSLGREAPQEFDVLAVDAFSSDAIPVHLITREA
                     LALYLRHVKAGGIVAFHVSNRFLDLVPVVARLAAENGVHAAVLYEEGEEGEARTLSDW
                     VLVSRDPAALKGEDLELAGVVPAEDRAALRTWTDDYSNLVQLLK"
     gene            complement(30288..31229)
                     /gene="secF"
                     /locus_tag="VFB53_09060"
     CDS             complement(30288..31229)
                     /gene="secF"
                     /locus_tag="VFB53_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZN86919.1"
                     /translation="MEFFKIRRVIPFMRHALVFNVISLITFLLAVFFLASKGLNFSVE
                     FTGGTLLELSYQESADVDRIRRALDQGGFKAEVQNFGSSRDVLIRMHPEKGQTSADLS
                     QKVMGLLQAEGKPVELRRVEYVGPQVGEELAEYGALALLVTAVGIVLYLWMRFEWRFG
                     LSAIIANLHDVVIILGCFAYFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRENLRKM
                     RKASVPEVIDSAITAVISRTIITHGCTQMMVTTMLVFGGPALHYFALALTIGILFGIY
                     SSVLVASPLVMWLGVDRAQFIRPAKNPDKDRNEGAVV"
     gene            complement(31237..33096)
                     /gene="secD"
                     /locus_tag="VFB53_09065"
     CDS             complement(31237..33096)
                     /gene="secD"
                     /locus_tag="VFB53_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZN86920.1"
                     /translation="MNRYPNWKYALVALCVAVAALYTLPNFFGESPAVQVSSVRATVR
                     VDAALLQRVESALAQAAISYSGAVLDANSVRVRFADTDIQLKARDTVEKSVNPDPANA
                     SYVVALNLLSSSPAWLTAINALPMYLGLDLRGGVHFLLQVDMPAALTKRLESLTGDIR
                     SLLRDKTVRHGGVTREGQSLRVRFRDAGTRAKARDIIAAGIGDLLLSEREEGADLLLV
                     AALKPEAAKRTQEFALKQNITTLHNRINELGVAEPVIQQQGADRIVVQLPGVQDTAKA
                     KDILGRTATLEIRMVDEEKMAPEILAAAARGEVPFGTEYYVERNGAPLLVRKQVVLTG
                     DRLTDAQPGFDSQTNEPAVHLTLDGTGARIFKEVTRENVGKRMAILLIEKGKGEVVTA
                     PVIRSEIGGGRVQISGRMSTTEANDIALLLRAGSLAAPMEIIEERTVGPSLGRDNIEK
                     GFNSTRWGFTAIAVFMTLYYVVFGLISSLALGVNLLFLVALLSMLQATLTLPGIAAIA
                     LTLGMAIDANVLINERIREELRGGATPQAAIAAGYERAWGTILDSNITTLIAGLSLLI
                     FGSGPVRGFAVVHCLGILTSIFSSVVVSRAIVNLAYGHRRKVERLHIGDTAWK"
     gene            complement(33107..33376)
                     /gene="yajC"
                     /locus_tag="VFB53_09070"
     CDS             complement(33107..33376)
                     /gene="yajC"
                     /locus_tag="VFB53_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZN86921.1"
                     /translation="MGLLPIILMFVLLYFLMIRPQMKRAKEHKQMVEALQKGDEVITA
                     GGMVGRISEMGDAYVTLEVAPNTEVSVQRAAVQTLLPKGTMKSLK"
     gene            complement(33459..34562)
                     /gene="tgt"
                     /locus_tag="VFB53_09075"
     CDS             complement(33459..34562)
                     /gene="tgt"
                     /locus_tag="VFB53_09075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZN86922.1"
                     /translation="MKFTVSHRDGAARRGRLELAHGAVETPAFMPVGTYGTVKAMSPA
                     ELLEGGTQIVLGNTFHLWLRPGTEVIARHGGLHRFMGWERPILTDSGGFQVFSLGALR
                     KITEEGVKFASPINGDRLLLTPEESMRVQRVLDSDIAMVFDDCTPHPATAKQAEKSMR
                     LSLRWARRSKDAFGGSPNALFGIVQGSVYEDLRAESLAGLEDIGFDGYAIGGLAVGEP
                     REARERILAHTAPRLPADRPRYLMGMGTPEDLIDAVLAGVDMFDCVLPTRNARNGWLY
                     TRFGDVKIRNARWRDDPQPLDAQCACATCRNFSRAYLHHLQKANEILGARLNTLHNLH
                     YYQELMRDLRTAIEGAGLAPAAARLREARRRLL"
     gene            complement(34559..35557)
                     /gene="queA"
                     /locus_tag="VFB53_09080"
     CDS             complement(34559..35557)
                     /gene="queA"
                     /locus_tag="VFB53_09080"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZN86923.1"
                     /translation="MRLAEFDYALPEELIAQRPAGARTSSRLLRLDASSGRIEDLAFA
                     DLPGLVGRRDTLVLNDTRVIKARLAGAKASGGRIELFVERVTGAREALALIRSSHHPR
                     EGGELLVGDGVRVRVAGRVDDLYRVRFEEDVDAVLERCGAVPLPPYIRHAPGAEDAER
                     YQTVYARQPGAVAAPTAGLHFDRGLLAQLEARGARIAPLTLHVGAGTFQPVRSEDIDA
                     HRMHQERYDIPRATMDAIAGRRVLAVGTTSLRALESAALTGQLRGETGLFVRPGFQFL
                     LVDRLLTNFHLPKSSLLMLVAAFAGMENIRRAYAHAIAARYRFFSYGDAMLIERAA"
     gene            35597..35683
                     /locus_tag="VFB53_09085"
     tRNA            35597..35683
                     /locus_tag="VFB53_09085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35631..35633,aa:Leu,seq:caa)
     gene            35789..>36329
                     /locus_tag="VFB53_09090"
     CDS             35789..>36329
                     /locus_tag="VFB53_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN86924.1"
                     /translation="MAKAQLLVLPAARNTAMVIDDQSTGRAILEQVVRSLDERVSVEG
                     FGRPVDAVVWATRHIADLVLIDYMMPEMNGIELATRLRALPGYEHVPIMMVTAHDDRK
                     VRYDALDAGVTDFLIKPVDARECMARCRNLLTLRRQHLALEDKRRLLEGMVEEATREV
                     RDRERETLMRLARAGEFRDE"
BASE COUNT         5526 a        12630 c        12815 g         5358 t
ORIGIN      
        1 cgggaaccgg cagcttccgc gactacctcg accaggtgga aaccgccagc tcgggcgaac
       61 gcgagcagtt cgtcaatgcg ctgaccacca acctgacggc tttcttccgc gagccgcacc
      121 acttcgagat gctcgcggcg aaggcgaggg gggccgggcg gttgcgcgtc tggagcagcg
      181 cctgctcgac gggcgaggag ccctattcgg cagcggtggc gctgcgcgag gccggttgtg
      241 cggccgagat actcgcgacc gacatcgaca ccgagacgct cgccactgcg cgcaatggcg
      301 tctaccggat cgacgcgctg gaggcggtcg gcatcgagcg catgcggccg cacttcctgc
      361 gcggcaccca tgagaatgcg cgcttcgcga aggtgcgccc ggagctgcgc ggcatggtga
      421 agttcgatta ccgcaacctg ctgtcgcagg actggccgcc gcgcgagcgc ttcgacgccg
      481 tgttctgccg caacgtgatg atctatttcg agcgtacggc gcagcggagc ctgctcgagc
      541 ggttcgccgc ggtgctcgct cccggcggac tgctgttcct cggccactcc gagaattgcg
      601 ccaccgggca tccgaagttc cgcgcctgcg gcaagacgac ctacctgcgc caatgaacga
      661 gaccgtcgcc ctgtacttcg accggcagtt cgaccgcgag gcggtcaaga tcctgcccgg
      721 ccagtactac gccgccgacc gcgacatcgt catcgtcacg gtgctcgggt cctgcgtctc
      781 ggcctgcctg tgggacccgg tgaagcgcat cggcggcatg aaccacttca tgctgcccgg
      841 ggccggaccg gcggccggcg cggcgggcgc cggagcgcgc atggggatct acgccatgga
      901 gctgctcatc aaccgcatgc tcaagctcgg cgccgagcgc ggccggctgg tcgccaaggt
      961 gttcggcggc gccagcgtgc tcgagggcat ggacgccctc aacatcggca gccagaacag
     1021 cgccttcgtg ctcgatttcc tgcgcgtcga gggcatcgcc ctcgcggcgc aggacctgta
     1081 cgacgtgtgc ccgcgcaagg tgtaccactt cccgcacacc gggaaggtgc tggtgaagaa
     1141 gctcggcgcc ctgcgcaacg acacgctgca gcgccgcgag cgcgactacc tcgccacgct
     1201 gaaggactcg gccggggcgg gcgaagtcga gatcttcggc tgccggtgat ggaccgcatc
     1261 cgcgtcctgg tggtggacga ttcggcggtg atgcgcggca tcatgagccg gatcatcaat
     1321 gcgcagccgg acatgaccgt cgcggccgtg gcgagcgacc cgctggtcgc aatggagcgc
     1381 ctgcgcaccg cgccgccgga cgtcatcgcg ctcgatgtcg agatgccgcg catggacggc
     1441 ctgcaattcc tcgccaggct gatgcaatgg cggccgacgc ccgtggtcat ggtgtcgtcg
     1501 ctgaccgcac agggcgcgga gaccacgctg cgcgcgctcg agctgggcgc cgtggatttc
     1561 gtcgccaagc cgctgctcgc gggcgggcgg gagttcgaag actatgcgca ggaggtggcg
     1621 gagaagctgc gcgcggccgc cggcgcccgg gtcgggcgca ggccgggcgg ggccgccgct
     1681 gcgggcacgc ccaagccgcg gcagggcgcg gccggcggcg gcagccgcga ccaggtgatc
     1741 gtgatcggcg cttccacggg cggggtcgag gcgctgcgcg aagtcctggt gccgctgccc
     1801 tgcggcatgc cgccgatcct ggtcgcgcag cacatgccgg cgggattcac ccgcagcttc
     1861 gcgcagcggc tcgatgcgct ctgccaggtc gaggtcaagg agggcgagga cggcgagccg
     1921 ctgcgccgcg gccacgccta catcgccccc ggcggcaggc acctcgaact gtgcgccagg
     1981 gccggcaagc tcgaggccag gctgacgcag gagccgcccg tcaaccggca tcgcccttcc
     2041 gttgacacgc tgtttcgatc cgccgcccag gccgcggccg cgcgctgcat cggcgtcatg
     2101 ctgtcgggca tgggcgccga cggcgccgac gcgatgctgg agctgcgccg ccgcggcgcg
     2161 cgcaacatcg cccaggacga ggcgagctgc gtcgtgtacg gcatgccgaa gcaggcggtc
     2221 gccgcgggcg cggtgcacga acttctgccg ctgcgcgaga tcccgtcgcg cctgatcgaa
     2281 ctgtgtcttg cgaatggctg acattggctg acgcttgcgc gccggcggaa agccatcatc
     2341 gccacgttcg aatagaccat tccgaccaac ttgaaacgaa gggagatgtc atgaggaaca
     2401 aactgatcta cgcgctgctc gccgccgcct ttgccggccc ggccgcagcc ggcgacaccg
     2461 ccacgctcgc cgtctcggcg gtggtcgcgg gaacctgcaa gttcaccacc gccacgttca
     2521 acatgagctt cggcacgctc gacccgtccg ccgcggcgaa ccagacgcag tccgcgggca
     2581 tcacctacaa gtgcaccaag aacgccgccg cgacctcgtt ctcgttcgac ggcgatgcgg
     2641 cttcgcctgc gtccgtgaac atcaccaacg gcatcgacaa cattccggtg gccctgtcct
     2701 ggaccgcgcc ggccacgctg ggctcgggct tcggcgccgg ctccagcgcg atcacgatga
     2761 acgtcaccgg cgacatcctc ggcaccgact acgccgacgt gtcggcgggc aactatacga
     2821 gaaacgtggc agtcgtcgtc gccccgtaag ggcgcaatgc taggcccgct tgcggcgcgg
     2881 gcgtccggca ggctccggct tgccgggcgc cggcgcctcg ccagcggcgg gagatggcgg
     2941 cgcagagcgc tccaggcgat aaccatgctg ataaacggcg ctcaggcgcc atccctcctc
     3001 cggatacagc ccgagcttgc tgcgcagctg gctggcgtgc gtatccacgg tgcgcgtggt
     3061 gacgtcggcg ctgatgcccc acacggtgtc gagcaggtaa gcgcgcgaca gcagccgccc
     3121 cacgttgcgg aacagaagca cggccatttc gaattccttc tgcgtcatct cgatcacctg
     3181 gccattcttg tgcagcgtgc ggccatgcgg gtcgatgcga tacgcgccgg cctcgatcgg
     3241 ctcctcggca tcgcgcccgg cgccgcgcgc gcggcgcagc agcgcgtcga ctcgcgccag
     3301 gaactccatg cggcgcagcg gcttgaccag gtagtcgtcg gcgccgctct tcagcgcatg
     3361 cacgatgtcc tcctcccggt cgcgcccggt ggtgaacagc accggcaggg gctcgcgcat
     3421 gttcttgcgg atcgccttga gcacctcttc gccgctcagg tccggcacgc gccagtcgag
     3481 gatcaccagg tcgaaggtat cgcgcagcag gacgcgcagg atgtcgttgc cgcgttcgaa
     3541 caggcggcag acgaatccct gcgaggtgat ccagtgctgc agcagcttgg cctgcgaggg
     3601 atcgtcctcc agcacggcga tgcgcggcat gccgccggca ggttttgcgg tgcgctctgg
     3661 atgcatgccg atgccatacc ccccgaagcg tattctaacg acagacccgg aaagaggtga
     3721 ccgcgtgacc ggcccccgca catccattct gcacgtcgag gacgacccga gtctgcaaaa
     3781 cttggtgcgc atcacgctgg agcagctcgg cggctacgcc gtgcgcaccg cggcaaacgg
     3841 ctaccaggcg ctcgacctgg cccgccaggc gctgccggac ctgctgctgc tcgacctgga
     3901 cctgccgggc atgaacggca ttgccacgct gcgcgcactg cgcgaaatcg atggcatggg
     3961 cgaggtcccg gtcgtgtttc tcaccgccgc gtcggacctg atgggcgacg cggaatttcg
     4021 ggcattcggc gtgcgcgaag tgctgccgaa accattccgg ccgcgccagc tggtgcaggc
     4081 gctggcccgc gtactcgggc ccagggggga ctgagcatgc agcccagctc gtacggcacg
     4141 cacgccacgc ccgacgacac cggcggcctc gctcgcctgg tggccgacaa cgccccctcc
     4201 acgctggtct acctggatgc cgacctgcgc attcgcttcg ccaaccggca ctgttacgag
     4261 ctgttcggcc gcacgccgcg cgaggtgctc ggccgcccgc tggccgacct gctcgacccg
     4321 cgcacgctca ggtacgcgct ggcgcatcgc gcggaggtcg agcgcggcaa tctcgcgccg
     4381 cgcgactacg tgctgagcga ccgggaaggc gacaggaaat tcgtcaaggt gcatgccgtt
     4441 cccaatcgcg accggtcggg gcgcagcgtc ggatatttcg cctgcacctc cgacaatgcc
     4501 gccgagcgcg ttacgcgcgc cgcgctgtct gccgccgagg agcgcctcag cttcgcgctg
     4561 gcggcctgcg aagcgggcat ctgggtgtgg gacctggccg cgcatacggc gcactacacg
     4621 gccgaattca aggccctgct cggctacggg gaaggcgaat ttccgccaga tttcggattc
     4681 ttcggcgccc tgcatgcgga ggacggcgcc gccatgttcg atgcggtggc cgcggcgatc
     4741 caggacggcg gcgtgttcga tcgcgagttc cgcatccgct gcgcggacgg cggttaccgc
     4801 tggctgcgct gcatcggccg cgcgatcgtc gacccggcga ccgggagcac cgtacgcctg
     4861 gcggggacgg tgcgggacat ttcgccgcgc aagcaggtcg aatcgcgcct gcgcgaggcg
     4921 caggcgctgg tgcaggcgac gctcgagggc cagctggcgg ccgccgagca gcagggcgag
     4981 cgcgaacggc tcgaccaggt gaagcgcgaa ctggtcgccg ccgccggcca cgagctgcgc
     5041 acgccgctcg cgtccatcat cgccgcgctg gaattgctgt gcgagggcaa ggcactgggc
     5101 acggagcggg ccgccgaatc gtttctgcgg ctggcgctga ggaacgccga gcgcctggcg
     5161 cgggtggtcg agcagtggct cgacgtggaa cgcatcgacc tgggagtgac ccgcgtccag
     5221 gcggtggcgc tcgacctggg cgcgctggtg gcacgcgtca tcgccgagcg cgccgcggcc
     5281 gcgcagcggc attccctgcg cttcgaaacc ggcggcgggc tcggcagcgt gctgatccgc
     5341 ggcgatgcgg aaaggctccg gcaggcggtg gcgcacctga tctccggcgc gatcgatcgc
     5401 tcgccgcccg gcgccgtggt gagcgtcagg atcgaggtgc acggcgacaa ggcgacattg
     5461 cacgtcgagg acgagggccc ggaactgttc tccggcgccg atctcgggct cagcctgtcc
     5521 aaggcgatcg tcgagcggct gggcgggacg ctgggctgca ccggccgcca gggcaagggt
     5581 gcggcatacc agctcgcatt gccgcggctg gcggccgcga actgaatcta cgaccggaag
     5641 gcgacacgat gcgcaacgaa gcactgctgg acctggaaac catccgcgac ctggccgagt
     5701 tcgacggcgg gccgggcgcg ccgttcgccg tggaattgct ggagatgttc acagcggacg
     5761 cccatcgcgc gctgctgcgc atgcgtgtca gcgcctggct cggcgacgcc ggcgcgcttg
     5821 cccgcgaggc gcaccgcctg aagggcagca gcgggtcggt gggcgcgacg cgcctgcagg
     5881 cagagtgcct ggcgctcgaa cgctgcgccc gcgagggaaa cctgggcgag ctggtcgggc
     5941 tcgaagagcg catcgagcac gccctggacg tgctgcaggc cacgcgcgag ggcatcctgc
     6001 agtgcgacgc cctcttccgc aaagcggagc gctgtagcac catggaacgg gcgatctgag
     6061 gaggacggct tggcgtcgag cacgaagcgc agttcggcac gcccgcgttt gcggccgggt
     6121 cgcggcagcg ccgcgcattc gcagcagcgc ggccggcgct tgcgcgagag ttccgcgcag
     6181 ttccggctgg cgcaggaggc gctcggcttc gtcacctgga tctgggacgt gcgcgacgac
     6241 cgcgtccagt ggtacggcga catgtcgcgc ctgctgggac tggcgcccgg cagcttctcg
     6301 ggacgcttcg aggactacct caccttcgtg cacccggagg acgttgccgg ggcgcgcgcg
     6361 accttcctcg actgcctcaa gggcaagcgc ccggagtacc gcgcagtgga gcgcgtggta
     6421 tggccggacg gcagcgtgca ctggctcgaa acctacggcc gcgccgaata tggcgctgat
     6481 ggacgcgccg tgcgcgcagc cggtgccatc aaggacatca gcgagaggaa gcggatggaa
     6541 tccgcgcgcc tcagggcgga acagcagctg gcgcgcgtgt tcgacgcctc gcccgactac
     6601 atcgcgatcg tgcgcgccag cgacgggcgc ttcgtcgccg cgaatcgcgc cttcgagcag
     6661 gtcaccgggt accgggcgga cgaggtggtc gggcgcacgg tcgcggaaat cggcatctgg
     6721 gcgatcccgg gcgagcgcga gcgcttcctc gcggacctgc acaagggcgt ggcggtgcgc
     6781 gaccggccga tgctgctgcg cgtgcagggc ggcaggatcg tctccggcac gatctcggct
     6841 tcgctcatcg agcacgacgg cgaacagctg atcatcggca tggtgcacga catcaccgag
     6901 gcgaaacggc tcgagcgccg cgcgcgccag tccgagcaca agttcgccgc gctgttcgag
     6961 aacagcccgg aagccatcac cctgtaccgg ctgtcagacg gcatgcggct ggaggcgaac
     7021 agcgcctggg agcgcaccac cgggtattca cgcgcccagg cgaaggggcg cccggcgaag
     7081 gagatgggcc tgtggaagga ccaggcgcag cgcgcggcgg tgatcgcgcg gatcgacacc
     7141 gagggcagcg tttccaacgt ggaggcgcgc ctggtgcgcg cggacggcag cgagctggat
     7201 gcgctcatct ccgccgtgcg cattgacctg gagggcgagg cctgcgtgct ctggtgctgg
     7261 cgcgacgtca gcgcggagcg cgcggtggag cgccgcgcgc agcaatccga gcgcaagttc
     7321 gccgcgatgt tcgagaccag cccggtcggc ctggtggtga cccggccgag ggaccggcgc
     7381 atcgtggaga tcaacgacgc ggcgctgcag ctgatcggcc tgggtcgtga cgaggcgatc
     7441 ggggcgatga cgaccgacgt cgtggagatt ctggaccagg agcagtttca gcgcatacgc
     7501 tcgcgcgggc tggcgggcga gcgcgtggcg ggcgagccgc tgcggttccg gcgacgcgac
     7561 ggcaagcagg tggaggcgct gatttccggc gccaccgtgg agttcgacgg cgagccgcat
     7621 ttcgtggtgt cgctcctgga tgtcaccgag cagcgccgcg tcgagcggga acgccagcag
     7681 gcggacgcgc gctaccgcgc gctgttcgaa tcgacctcgg acagcatcgc catcttctcg
     7741 ccggactgga agttcatcga cctgaatgcc ggcaaccgcc ggctgagcgg gtatgcccgc
     7801 gaggaactga tcggccagcc ggtgaccatg gtgctcgacc aggaggatct ggcgcaaaag
     7861 ccgatccgcg acgaccgcga ctggacgctg atcgaacgca ccctgcggcg caaggacggc
     7921 agcaagcgcg ccatcgaggt gatgtccggg cggctgccgg acggcaacat catcgtcatc
     7981 gctcgggaca tctccgagcg caagcgcagc gagaccctgc tgatgaacgt ggcgcgcggc
     8041 gtctcggcgg agctcggcga ggccttcttc cgctcgctgg tggagcacct ggcgcgcgag
     8101 ctcgacgccg atttcgcctt catcgccgag gtggtgcagc cggacaacga gcgctgccgc
     8161 accctggcct acgtcgccga cggcgcgatc gcgccgaatt tcgagtaccc cctcgcgggc
     8221 tcgccctgcc tcaacgcgat cacgcaacgc ggcacggtga tgtacccgca aggcgtggcg
     8281 cagcagttcc cgctggacgc cgggctcggc aggaacggca tcgagggcta tgtcggcacc
     8341 tccctgcacg gcgccgacgg caccgcgctc ggcgtgctgg tggtgatgca ccgcaagccg
     8401 atcgagcgca gccagttctg ggcgtcgatg atcgagatct tcggcgctcg cgcggcggct
     8461 gaaatcgagc gctcgcgggc ggaggcgctg gtgcgccgga ccaacgcgtc gctggagcag
     8521 gtggtgcacg aacgcacgtc gcagctggaa gaggccaacc gcgacctgga gagctacaac
     8581 tactcgatct cgcacgacct gcggcaaccg ctcaacgcca tcgccggctt cgccgagctg
     8641 gtgcgcgaca ccgccggcag cgcgctgcag agcggaacgc gcgaattcgt gcgcgagatc
     8701 gagagcaatg ccgagcgcat ggagaggatg atcgaggcgc tgctgcagct gtcccgcgcc
     8761 gggcgcggac ccctggacaa ggccgaggtg cagatgggcc cgctggtgga ggccgtgctg
     8821 cgtgacctgg gcgtcggtgc gccgctcgcc gcgcagatcg aggtgggcga gctgcccgcg
     8881 gcgcagggcg acgaagtcct gctgcgccag gtctgggcca acctgatcgg caatgcactg
     8941 aagtactcgc ggctgagccg ggcgccgcgc atccgcatca gcggcgcgcg gcgcgacgga
     9001 ttcgtggagt acgagatcag cgacaacggg gtcggcttcg acatgctgca tgccgagcgg
     9061 ctgtgcgaga ccttccaccg gctgccgtcg gcggtttcgt tcgagggcaa cggagtcggc
     9121 ctggccatcg tgcagcgcgt gttgcgccgg cacggcggca gcatccgggc ggaatccgcg
     9181 cctggcgcag gggcgacctt ccgcttcaca ctgcctgacg aagcccttgc atagcctgcc
     9241 aatcgcgccg cctgcgctgg ctacagtggc gcatgcttcg cgatgccgca gtccgattgc
     9301 tcctggcgac agggttcctc gccgccgcgc cgcagttgcc ggcgcatgcg gcggagccgt
     9361 cgatctcgct ctactgcgcg cggggcgtga ctgactatcc ggatgccgaa ctcacgctga
     9421 gcacccgggg cagccacctc gtcaaggtgg tgttcgtcgg ctaccggccg tcgcgcgcgc
     9481 gggccgactg gagcctgcgc gattgcctga gcaccgcgat caagttcgac ggctcgcggg
     9541 acatcgtcgc ttcgctctgg taccgggacc gcgcgctgcg cagtccgccg gagccgctgc
     9601 ggcccgacgg cgccgccgcg agcctggtct accaggcggc acgccatgcg gtcgtagtgc
     9661 agaggcggga aaccaggccc taggcccgct tcacactgcc tgacaaagcc cttgcacagc
     9721 ctgccaatcg cggggcgtgc gctggctaca gtgccgcatg tcccgacgcc tcctcgccgc
     9781 agttttctgg ctcgccacga cctgcgccgg cgccgcggag ttcagcgtgt cgccgatccg
     9841 catcgagctg ccgcgcggcg cgcgctcggc cgccgtatcc gtggcgaacg aggaccggca
     9901 gccgctgcgc atgcagttgc gcctcatgga gtggacccag gacagcgacg gcaaggacct
     9961 gtaccgggag agcgacgcgc tcatctacta cccgcgcctg atgaccattc agccgcgcga
    10021 aaagcgcctg gtgcgcatcg gcgtgaaggc ggccagcgcc gcggccgagc ggacctaccg
    10081 cctgtacatc gacgagctgc ccggggtggc cgacgacccc ctgcggccgg ccgtctccgg
    10141 gatcaatttc acgctgcgct tcgccttgcc cgtcttcgtg ccgcccgccg agccggcgct
    10201 gcgcggcgtg atcgaagcga tgtcgctgca ggacggcaag ctggccgtca cggtgcgcaa
    10261 cgccggcaac cagagcttcc gcatcgcctc ggtcgcggcg cgcggcggcg catttgccgc
    10321 cgaggccggc ggctggtacc tcctggcggg cgcggcgcgc gtgcaccgct tcgacatccc
    10381 ggccgaggct tgccgggaac tgcgccggat cgaggtcgcg gtgacggccg acaggctctc
    10441 gctcgaaggg ggcctggatg tcgatccggg catgtgtcct cgctgagctc gccgccgcgg
    10501 ccctgggagc ctgcggcctc gccgcagccg cagaggagcc gctgaggctg aaactggcgc
    10561 ccgcgctgtc gcaggcgcgc ccggcgctcg agccgctcat cgtcgaggtg agcgtcaacc
    10621 agcaccggcg cggcgagttc acggtctacc gggacgccga cggcgactac tacctgcggg
    10681 cctccgagct cggcgccctc ggcctggccg agcatgccgc tgcgccgcgc acggtgcaga
    10741 tcgacggcga ggcgttcgta tcgctgcgcg ccttcgcgcc gcgcaggctt ttccttgacg
    10801 aatcgcgcct cgccctggag atcgagtttc ccgcacctgc gctgcgcaag agcacctacg
    10861 acctgatgcc gcgccggccg gatctgccgc aggggccgcg cgaaccgggg ttcctcaatt
    10921 accgcctgag cgcgagcgac ggcagcggcg cggagcggcg ccggttcggc ctggcgcatg
    10981 aactggcggt gcgcgcgggc gacgttctgt tgcgccacga agccgcgctg ctgcacggcg
    11041 ccggcggcac gcgcggcctg cgttatgcca cgcagatcgt gcacgaccgc ccggcagagc
    11101 agcagcgcct ggtgctcggc gaccacgtgg cggcgagcgg cgagctcggc tcgacgctcg
    11161 ccgtcggcgg catcagcttc gccaagcact acccgctggc gccgcatttc atccgccagc
    11221 cgctcgccgg ctacgccggc gccgtcagca ccccttcgca ggtcgaggtg cgcatgggcg
    11281 gcgtgccggt gttccgcgaa caggtgccgg cggggccgtt cgagctgcgc aacctgcagc
    11341 agttcgccgg cgcgcgggac gtcgagatcg tcatgcgcga cgccatgggg cgggagcaga
    11401 cgatcggctt tccgcattac ttcgccgacc aggcgctgcg ccggggactg cacgaataca
    11461 gctacagcgc cggcgcgctg cgcgaagcgc tcggcgtgcg cagcaatcag tacggcgccg
    11521 gggtgctgac tgcggtgcac cgctacggcc tgagcgaccg cgccaccctc ggcctgcgcg
    11581 ccgaggcggc cgagggcctg ggcaacgtcg gccccacggc gctctaccgc gacgaccgcc
    11641 tgggcgtgtt ctcgggcgcc ctggcgctca gccggcgcga ggggcgcagc ggcgtcgcga
    11701 ccgcgctcgc gcatgcctac cagacgccgc gcttcggcct gcgctcgagc gcccggcgct
    11761 acagcgcgga ttacgcggtc gcgcaggacc tggtggcgcc cgcctggctg caagcggagt
    11821 acggcctggg cggatcgctg ctcctgggcc ggttcggctc ggtgttcctg gaccgctcga
    11881 tcgtcaagcg gaacgcgagc gtatccctgc acgacagcgc gacgacgcaa ctgagctata
    11941 cctacagcct gggccggcag ggttcgctgt tcgccacgct cgcccgtctg cacgccgagc
    12001 gcaccgacac ccgggccttc gtcggcgtgc tgattgccct ggaccgcgcg aagaccgcgc
    12061 acctgagcgc ccggcgcgag ccggacggcg acgcggccct ctacggcgcg caactggcca
    12121 gcacggtgcc ggcgggcgaa ggcctgggat accgcgtcgg cgtcgacggc atcggcggca
    12181 gctcgaagca gtggagcgga tttgcgcaat acaacgcgcg cgccgcgagc ctgacgctcg
    12241 atgccacgag cacgcgcgcc gccggcgagg gcgccgggcg ctacgaaatc gccgtcgccg
    12301 gcgccgccac ctgggcaggc gggcgcctcg gcctggcgcg ccaggtcgag gacagtttcg
    12361 tcgcggtgca gctcgccgcg ccgctggaag gcgtgcgcgt ctacagcaac aaccaggaag
    12421 tcggccgcac cgaccggcat ggccggctga tcgtgccgaa tgtcggctcg ttccacgaga
    12481 cgcagatcgc catcgacgaa tccgacgtgc cgctcgatca cgcgatcggc gcgatgcgcc
    12541 tggtggtggc cccggcctac cgcagcggtt cgctgctgcg cttcgacgtg cggcgccagc
    12601 gggcgatcga aggcgtactg ctggtgcgct cgcgcgaggg actgcggccc gccgagaatg
    12661 ccctcgtgcg cgtcggcgat cgggaattca ccactggccg cgacgggcgc tactacgtcg
    12721 aggacctgcc gcccggccgt caccaggcgc ggcttggcgc ctgcgcattc cagctcgtcg
    12781 tgcccgactc ggcggagccc gtcgccacgc tgcccgaggc cgtcgcctgt gagtagattc
    12841 ctctgcgcgg cgcttctcat cctggcgccc agcctggcct gcgcgcaagt cgcttgcggc
    12901 tttctcagca cgcctggcgc cgctttcggc gtgtacgacg acggcgcgcc aggcgccgcc
    12961 gacacggcaa cctcggtcgt ggtgcgctgc ctgcgcaacg gcggcccggc caatgtgacc
    13021 ttgacgctgc agatcggccc gagcgcgggc agcggccaga tcgccagccg cgcgctcagt
    13081 gccggcgccg agcggctggt ttacaacctg taccgcgacg cgtcgcggtc tgcggtatgg
    13141 gggcagacgg ccggcgtcga caccggcgcc gtcgcgctca acggcatacc caacaacggc
    13201 tcgaaggacg cgactttcgt ggtttacggc cgcatccctg cgctgcagag cgtagccgcc
    13261 ggcgcctacg ccgacagcgt gcagctgacc atatcgccct gatacactcc ggggcgtgct
    13321 cgcccggttg atcctctgcg cattgatctg ccctgcccca gcggcgcagg cggcggacct
    13381 ggggatcact gctgttgtcg cgcgcagcgc gcgagtcacg ctcctggacc agccgtcccg
    13441 ggtcatcgtt accccggagg acgtggcgcg cggctatgtc gatctgcctt ccggcccgcg
    13501 cctgggcgtc aggagcaata gccgagaagg catcgcggtg gcggtggtct cgacccaccc
    13561 gctgctcggc atcgagctgc gcggcgggca caccccgctc gtgctgcctg gcggcgtgcg
    13621 cagcctcgac ctgcattacc gcgtgctgct cgcgccggat gccctgccgg ggatctacgc
    13681 ctggcccggg cagatcatcg cgactcctct gtagggtcct cgggcggctg cctggccgct
    13741 tcttccttgg tgggcacgcg gtagtcctcg gacgcccact tccccaggtc gatcagccgg
    13801 cagcgctcgc tgcaaaacgg ccgccacttg ttgtcgtggc tgaacgccgc agcggcgccg
    13861 cagcgcgggc aggggactgt ctttacaggt tgcagaaggt gagctcgaag gccacgtccc
    13921 agtccgcggc gcgcggcttg ggatcctcgc tggcctgcgt gaggaagcgg atgttgagca
    13981 cgtacttgtt ggcgctgatc tcgggaacgc agttggccgc cgggtcgagg cgcacgcgca
    14041 tcatctgcgc ggacttgccg ccgagcatct gctggaacgt gcccagcggg gcggcgagct
    14101 tctgcggctt gcccgactcg cgcaggatgc gcaggatgat ggccgagccg tcgcgcagcg
    14161 ggaacagcgg cccggtccac gccgccagct ggccgatgcg ctcctcggcc gggcgatgca
    14221 gccagtaatg gtaggagggc aggtcgaact cgcaggcgcc cccgggaatg ccggtgcgct
    14281 gcttgatcgc catcagccaa tcgttctcgc ggatgtactg gccgatcctg ccggccatcg
    14341 agaacagcgc cgccgccgcc tgctcgatgt cgcgcagcac gctgctgagc gcctcctcgg
    14401 agatctccgg gttgttgcgg aaggacagca gcacctgctt ttgccgctcc agttcctgca
    14461 gcaggtccga tttcaggtcg gcgcggctgg acacctcgag gatctcgaac agcgtgagca
    14521 gcgccacgtg gtgatcgagc ggatcggtgc gcgcgatgaa gtggcgcgag cgctcgaaca
    14581 gatcctccag ccgcagcagg gtccggatgc gctcgttaag cgggtactcg tacgtaatca
    14641 cgtccgttcc cggctggcag aaacctggcg gattctgagt gatttggcgg gggatgacaa
    14701 attcacggcg cggcgcgggc gcgttgcagg tagtgcgcgt gcagttcgcg cacctgcttg
    14761 tgcagtgcgt caagcgtgcc cgaattgtcg atcacgtcgt cggccgcggc gagccggact
    14821 tcccgcttga tctgggcgcc gatgatgcgc ccgacctcgt cggcggacag gccgctgcgc
    14881 tcccgcaccc gcgccagctg cacttccggc gggcaatcga ccaccagcac gcgcgccacg
    14941 cgctgccggt atcccgcgga ttcgaccagc agcggcacga cgtggatcac gtacggcccg
    15001 gccgcggccg cgatgcgccg ctcggattcg gcgcggatca tcgggtgcag cagcgcctcg
    15061 agtgccttgc gtgccgcgcc atcccggaac gccagctccc gcatcctggc gcgatccatg
    15121 gcccccgcgg catcgacgta ggcctcgccg aactgccggc gtatcagcgg catggcggcg
    15181 ccaccggcct gcgtcaactc gtgcgcgatc gcgtcggtgt ccaccagcgt cgcgccgagc
    15241 cgcccgaact cctcggccgc ggcgctcttg ccgctgccga tgcctccggt caggcccacc
    15301 acgtaagcca tcaacccagg atcggcagcc agcggcgaac gatgtcttcg ccccagaaca
    15361 gagcgatcag cccggcggcg gccaggtagg ggccgaaggg aatcggcttg tcgcgcccgt
    15421 ggcgcgcgag caccatcaag ccgatgccga ccgcggcccc gaccaccgag gacaggagga
    15481 tcaccagcgg cagcttctgc cagccgagcc aggcgccgat cgccgccagc agcttgaagt
    15541 cgccgtagcc catgccttcc ttgccggtgg cgagcttgaa tccccagtac accagccaca
    15601 gggcgagata gcccgccacc gcgccgatca gcgcggtgcg caagggcacg aacgcgcctg
    15661 tcatgttgat gagcaggccc gcccacagca gcggcaaggt gatgtcgtcg ggcaggagct
    15721 gcgtatcgag gtcgatcgcg gtgagcgcga gcaaggccca gccgaagccc atggcgcccg
    15781 ccgcggcgag cgtcggcccg aagcgccagg cggcataggc gccgatcagg ccggcgagga
    15841 tttcgattgc cgggtagcgc gccgagatgc gcttgccgca gccggcgcac cggccgcgca
    15901 acgccagcca gctgaacacg gggacattgt gcagcgcctt gatgcgcgcg ccgcaggcgg
    15961 ggcaggcgga gcgcggcacc acgaggttgt agcggcccgc gggcgggagc gccgtgccgg
    16021 cgaggtcggc gcactgctcg cgccactcgc gctccatcat cttcggcagg cgatggacga
    16081 cgacgttgag gaaggagccg acgcacaggc ccaggatcag cgccatccag ggcaggaccg
    16141 ctggctggac cagccagccc gagaccgcca tctagacggc ttggcccatc ttgaagatcg
    16201 gcaggtacat ggcgatcacc atgccgccga tcagggtgcc gaggaccacc atgatcatcg
    16261 gttccatcag cgaggacagc gcctccaccg cgtcgtccac ctcggcctcg tagaagtccg
    16321 ccaccttgct gagcatcgaa tcgagcgcgc ccgtctcctc gccgatcgag cacatctgca
    16381 gcaccatgtt cgggaacagg ccggaattgg tcatcgccat ggtgagcgag gtgccggtgg
    16441 acacctcctg ctggatcttc ttggtgccgg tgaggtacac gttgttgccg gaggcgccgc
    16501 ccaccgaatc gagcgcctcg accagcggca cgccggcggc gaacatggtg gacagcgtgc
    16561 gcgtccagcg cgcgatggtc gacttcagca ccaggtcgcc gaacaccggc agcttgagca
    16621 tcaggcggtc catgaagatc tgcaccggca gcatgcgctt ccaggcctcg aggaagccga
    16681 tcaccgcgcc gatgatcacc gggaagatga tgtaccagtt ggccacgaac cagtccgaga
    16741 tcgccatcac catcagcgtc ggcgccggca ggtcggcgcc gaaattgctg aacacctcct
    16801 tgaacgccgg gatgacgaag atcatgatca ccgcggtgat gatgaatgcc accacgatga
    16861 tggcgatcgg gtagaacagc gccgacttga tcttcgactt gatggcgagg atcttctcct
    16921 tgtagctcgc caggcggtcg agcagggtct cgagaatgcc ggcctgttcg ccggcggcga
    16981 ccaggttgca gaacagcgcg tcgaaataaa gggggtactt gcggaacgcc tgcgccagcg
    17041 aggagccggt ctcgacctcg gtcttgatct cctgcagcag gcgggccacc gccgggttgg
    17101 aggcgccctt gccgacgatg tcgaagcatt gcagcagcgg cacgccggcc ttcatcatgg
    17161 tcgccagctg gcgggtgaac agggtgatgt ccttctcctt gaccttgccc ccgctgccgt
    17221 acttctgctt cttgaccttc tgcaccatga tgccctggcg ccgcagggaa gcgctgaccg
    17281 cggtctcgct caccgagcgc agctcgccgc ggatcacctt gccgcccttg tcgcggcctt
    17341 cccagacgaa attgaattcc ttgggtccgc cggccttcgc cgcagctgcc gttgccatcg
    17401 cgcgttctcc gatcccttcc ccgttaccgc aggactgacc ggcgatgatg ccctgcggcc
    17461 agttttttgt aaagcctcag gtttcgcacc taaaccgctg acgcgccttg gttttgaggc
    17521 acgaaagtcc cctgcaaatc ccccctccgg cggggtgcct cacggcagct cctgctggct
    17581 gtggagctgc ggctgcgggc ggaacaggcg caccgtgcgg atggtgcggc cctgcgcgtg
    17641 cacgatctcc atcgccacct cgccgacctt gatcgacacg tcggcctcgg gaatgtcgcg
    17701 cagctgctcg aggatcaggc cgttgagcgt tttcggcccc tcggtgggca ggttcaggcc
    17761 gagcgcccgg ttcacttcgc gcaccggcgt ggcgccgtcg gcggtcgccg tgccgtcctc
    17821 ggaccaggcg agcgcctgcg acgccgacgg catcgaggtg gtgaacttgc cgatgatctc
    17881 ctcgatgatg tcctccaggg tcaccagccc catcagctcg ccgtactcgt cgaccaccat
    17941 cgcgatgcgc tcctcgttct cctggaagta ctgcatctgc gcgaacaccg gcgtggtcgc
    18001 cggcacgaag tagggcggca ccagcagcga ctcgatctcc gccttgttga gcgtgccggc
    18061 ctgcagcggc gacagcacct tgcgcaggtg caggatgccg agcacctcgc cgcgcgaatc
    18121 gcggaacacc ggcagccgca tgtggtagct ggtggcgagg tggcgcgcga tggcctccat
    18181 gtcgtcgtcc agccggatcg cctcgatgcg cgcgcgcggc agcatgatgt cctgcacggt
    18241 gattgcgccg aggtcgaaca ggttgagcag gatcgacacg tgcttcttcg gcatgaagct
    18301 ggaggactcg agcaccagcg cgcggatctc ctcgggcgac agcggctgcg cgcccgattc
    18361 catcggccgg atgcgcagca ggacgagcag cggcttgagg atcgcctcgg tggacagcga
    18421 cagcaggttg acgaagggcg ccgaggcgcc cgccagcagg cgcatcggcc aggccaccag
    18481 cctggcgatg gcctcggaat tgtgctggcc gaggcgcttg ggcaccagct cgccgacgac
    18541 gatggagaag taggtcaccg ccatcaccac caccgccgtg gcggcgatgg cgctggtctt
    18601 gtgctccagc cccagcgact gcagccagac cgccaccggg gcggcgagca ccgcctcgcc
    18661 gatgatgccg ttgaggatgc cgatcgacgt gatgccgatc tgcacggtgg acaggaagcg
    18721 cgtcggcgcc tcgaccagcc gcagcgctgc ggccgcgagg cggtcgccgc ccgaggcgag
    18781 cgcggcgagc cgtggccggc gcgaggtgag caccgcgact tcggacatgg cgaacagccc
    18841 gttgagtagg atcaggcagg cgaggatcag gatctccatg cgtgcacctc aggatcggcc
    18901 cagcagaact tccatcacga agcggctgcc gacgtaggcg agcagcagca tcgcgaaccc
    18961 gctcagcgtc cagcgcaggg cggtgcgccc gcgccagccg cggaaatgcc ggccggcgag
    19021 cagcagggcg aaggtcgccc aggacagcag cgcgaacagc gtcttgtgat cgaagcgcag
    19081 cggccgcccg aacagcgcat cggcaaaggc gaagccggtg gcgagcgtgc aggtgagcag
    19141 aacgaaggcg gcggcgatca agcggaacag caggcgctcc agcgtcagca gcggcggcag
    19201 cgcgccgagc ggtccttgca gcatggccac gccgtcggcg agcagcgcgc cgccggcgcc
    19261 gcgcgcctgc cgcgtctggt gcagacgccg ctcgacgacc gccatcagca gggcgtgcag
    19321 gatggcgatg gtgaacaggc tgtaggcgag catcgccagc accaggtgca gcctgaactc
    19381 cagcgaatcg gcgtaggcgc cgctggcgag gccggggaac agcgcaggca gcggcacggc
    19441 cagcgccgcg agggcgagcg cgggcgcctg catgccctcg aggttgtaga acaggctttc
    19501 ggcccagtag aacagcaccg ccagccacag catggcggag agcgcatggg cgaagccgaa
    19561 gcgcagcgcg cccgcgaaca gcaccgcgtg cagcagccag ccgtgcagcg ccagcgcggc
    19621 gaggatcgca gcgcgttccc aggggcgcag gccgccgccg ggcgccgcgc cgcccgcgga
    19681 ccgcgcccgc cagcgcgaat tccagaagtg ccacgccagc cccgcgtagc cggccgcggc
    19741 gagcgcgtga attacaatgt cgggcaccca accagtctat cacccgtgct cgacaacctg
    19801 accgcgcgcc tggcgaagat cgtgaagacg atgcgcggcg aggcgcgtct caccgaaggc
    19861 aacgtgcagg aggcgctgcg cgaggtgcgc gtcgcgctgc tcgaggccga cgtcgcgctg
    19921 ccggtggtgc gcgatttcgt cgccgcggtg aagcaaaagg cgctgggcgc ggaagtcgtc
    19981 ggcagcctga cgccgggcca ggcgctggtc ggcgtggtgc atcgcgagct cgcgcgcctg
    20041 atgggcgaga cgagcgtcgg gctggacctc gcggcgcagc cgccggcggt gatcctgctg
    20101 gcggggctgc agggctcggg caagaccacc accgcgggca agctcgcgcg cctgctgcgc
    20161 gcgcagaaaa agaaagtgct gctcgtgtcc tgcgacgtgg ttcggcccgc ggccatcgag
    20221 cagctggcgg cggtggcggc gcaggccggc gccgacgtgt tcccttcagc cgcaggcgag
    20281 cagcccgccg acatcgggcg gcgcgcgctg gagcacgcga ggacgcgcta ctacgacatt
    20341 ttgctggtgg acaccgccgg gcgcctcgcg gtggacgagg cgatgatgca ggagatcgcg
    20401 gcactgcacg cggcgctcag gccggtggag acgctgttcg tggtcgacgc gatgcagggc
    20461 caggacgcgg tcaacgtggc gcgcgccttc ggcgagcggc tgccgctcac cggggtgatc
    20521 ctcaccaagc tggacggcga cgcgcgtggc ggcgccgcgc tgtcggtgcg ccaggtcacc
    20581 ggcaggccga tcaagttcgc cggcgtgggc gagaagctcg acgcgctgga gccgttccat
    20641 cccgagcgca tggccgcgcg cgtgctgggc atgggagaca tcctgtcgct ggtcgaggag
    20701 gcgcacaagc aggtcgacct gaaggaggcg cagcgcgtcg cggagaagct gaagaagggc
    20761 aagggcttcg acctcgagga cttcaagcaa cagctgctgc agatgcagaa gatgggcggc
    20821 atggccgcca tgctcgacaa gctgccggcg cagctcgccg gccagatcaa tccgggccag
    20881 ctgcaggacg agcgccagct gcggcgcacc gcgggcgtga tcaatgccat gacgccgcag
    20941 gagcgcgcgc atcccgagct gatcaaggcc tcgcgcaagc ggcgcatcgc cgccggcgcc
    21001 ggggtgccgg tgcaggaagt gaaccgcctg ctcggccagt tcgagcagat gcagaagatg
    21061 atgaagatga tgaagggcgg caacctggcg cgcatgatgc gcggcctgaa gggcgccctg
    21121 cccgggatgc gctagctcag agccagccgg acttgcggaa gcggtagaac aggtaggaat
    21181 cgagcgcgac catcactgcg atggtcaccg ggtagcccag ggaccacttc aactcgggca
    21241 tgttctcgaa gttcatgccg tagacccccg cgatcatggt gggcaccgcc accagggcgg
    21301 cgtaggcggc gaggcgcttg gtgttttcgc tctcgccgat ggtgatcatc gccaggttca
    21361 cctggatcgc ggtggtgact gtgtcgcgca gggtgtcgat cgactggttg agccggaaca
    21421 ggtggtcgta gacgtcgcgg aagtattccc ccaatccggc gcagacctgc ggcacgcgcc
    21481 cgccgtacag gcgcgcggag gcctccacca gcggcgccac tgcgtgcttg agcgtcatca
    21541 gcttctgctt cacgtaatac agcgcctcga tgttctcgcg cggcgacttg ccggagaaga
    21601 tcttctcctc cagcgcttcc agctcgacct ccagcaggtc gagcaccggg aaatagcggt
    21661 ccacgatcgc gtccatcagc gcgtacagca cgtagcccga gccgtggcgc agcagctcgg
    21721 gttcgcgctc gcagcgcgcg cgcacttccg caaagccgcg ctcggagtgc tgccgcaccg
    21781 acagcacgta gttgcggccg acgaacacgt ccatctcgcc gaccttgagc tcctcgcccg
    21841 ccgcttcgac cgtgtgcagc acggcgaaca gcgaatcgcc gtactcctcg atcttggggc
    21901 gctggtggcc gtggcgcgcg tcttccaccg ccagctcgtg caggtcgaat tcgtcctgca
    21961 tttcggccag ctccgccgcg tcggcgtcct tcagcgccac ccagacgaag cagtccgggc
    22021 ggctgaggta ggacttgatc tcgcgccggg tgatctcggc gagtttcctg ccgtcctggt
    22081 aggcgacgca gttgaccagc atttggccga atcgtagcac tgtaaaatgc gcgccagagg
    22141 gagaacgcca tgccagccat cagccgccgg gcccagggcc tcggcaccga aaacgccttc
    22201 gtcgtgctcg ccgaggtgaa cgcgctcgcg cgccagggca aggacatcgt cagcttctgc
    22261 atcggccagc ccgacttccc ggcgccggag aacgtgcagg aggcggcgat ccgcgccatc
    22321 aaggggggca agcacggcta cacgccctcg gcgggaatcg acgagctgcg tgccgcggcg
    22381 gccaggtaca tggccggaat gcgaggcggc ctgccgatcc gggccgagga cgtggtggtg
    22441 ggcgccgggg ccaagccctt catcgcctac gccatcctca gcaccaccga ctacggcgcg
    22501 ggcgacgagg tgatctatcc caacccgggt ttcccgatct acgaatcgca gatcgccgcc
    22561 aacggggcgg tgccggtacc gatccacctg cacgaggcgc gcgatttcgc cttcgacccg
    22621 gcggagctgg agcgcctgat caccccgaag acgaagctcc tgatcctcaa caccccgcac
    22681 aatcccaccg gcggcatgct ggcgaggaag gacctggagg cgatcgcggc gatcctgcgc
    22741 aagcacccgc aggtatgggt ctacgccgac gagatctatt cgcgcctgtg ctacgcgggc
    22801 gagttcctct ccatcgccca gctgccgggc atgtacgagc gcagcatcat ttccgacggc
    22861 gcgtcgaaga cctgggccat gaccggctgg cgcatcggct tcacctccaa cccggtgctg
    22921 gcgccgcatt tcacgcgctg gatcaccaac accgagtcct gcgcctcgca gatctcgcag
    22981 tgggcggcgc tcgaggccat caacggcccg caggacgcgg cggcgatgat gcgcgcgagc
    23041 ttcctcgagc ggcgcgacct gatcgtggag ctgctcggca aggtgccggg cgtcgcctgc
    23101 cgcaacccgg gcggggcgtt ctacgcctgg ccgaacgtca ccgacgcctg ccgcatgatc
    23161 ggcgcggcgg attcggagga gttccgcaag cgcctgctgc acgaggcggg cgtggcggtg
    23221 ctcgccgaca tccatttcgg ctcgcgcgtc ccgggcgaag gacagcacct gcgcttttcc
    23281 tacgccgcct cgaaggcgca gatcgaggcg ggcgtcgcgc gcatggccga attcatccgc
    23341 aagaacacgc gaaaggcggc gtgacccctc cgtccaggag gggtcgccgt aaggcagggg
    23401 aggtccgatg acgcgctggc gcccgagcgt caccgtcgcg gcggtgatcg agcgcggcgg
    23461 caagttcctg ctggtggagg aggaggccag cggccggcgc gtgctcaacc agccggccgg
    23521 ccacctcgac ccgggcgagt ccctggtgca ggcggtggtg cgcgagacgc tggaggaaac
    23581 cgcgcaccgc atcacgccca ccgcgctgct cggcatctac cgctggcgct acgacaagga
    23641 ggaggacgtc accttcctgc gcttcgcgtt cctcggccgc gtggagggcg tcgaggcggg
    23701 ccgccagctc gaccgggaga tcatcgcggc gctctggctc acgccggccg agctggcggc
    23761 gcggcgcgcc gagcatcgct cgccgctggt gcagcagtgc gtggacgact gcctggcggg
    23821 acgcagcttt ccgctggatg cgctgtccag ggcctatgcc tgaaaaagtc gtcgtcggca
    23881 tgtccggcgg ggtggactcg tcggtggccg cgctgctcct gaagcgcgcg ggccacgagg
    23941 tggtcggcct gttcatgaag aactgggaag acgacgacga cgacgcgtac tgctccacgc
    24001 gccaggactt cctcgacgcg gcagccgcgg cggaggtcat cggcgtcgag ctcgaggcgg
    24061 tgaactttgc cgccgagtac cgcgagcgcg tgttcgccag cttcctcgcc gagtactcgg
    24121 ccgggcgcac gccgaacccg gacgtgctgt gcaacgccga gatcaagttc aaggccttcc
    24181 tcgaccacgc catgcgcctc ggcgctgcgc gcatcgccac cggccactac gcgcagctgc
    24241 gccaggcggc cggcaaggtg gagctgctgc gcggcgcgga cgcgggcaag gaccagagct
    24301 atttcctgca tcggctgacg caggcgcagc tgtctcgcgt gctgttcccg atcgggcatc
    24361 tgaagaagac ggaagtgcgc cgcatcgccg ccgccgcggg attgccgaac cacgccaaga
    24421 aggactcgac cggcatctgc ttcatcggcg agcggccgtt ccgcgaattc ctcaaccgct
    24481 acctgccgaa gacgcccggc gcgatggtgc atcccgacgg caagcgcgtg ggagagcaca
    24541 tcggcctggc cttctacacc atcggccaga ggaagggcat cgggctgggc ggggcgggag
    24601 agccctggta cgtggccgag aagcgcatgg cgaccaatga gctggtggtg gtgcagggcc
    24661 acgaccaccc gctgctgctg cgcaggtcgc tccaggggct ggatgcaagc tgggtattgg
    24721 cggcgccggc ggcgggcgcg gcgcatgccg ccaagacgcg ctaccggcag gcggattccg
    24781 cctgcaccgt gacgcgcgtc gggcaaggcg cgctcgcggt ggaattcccg caaccgcagc
    24841 gcgcggtcac gccgggccag tcggtggtgc tctacgacgg cgaggtttgc ctcggcgggg
    24901 cggtgatcgc ctagacggcg ggcacgctgt cggcgaaagc cgctcgttcc atcagcttgt
    24961 cgtagtgctt cgccaggccc ggatactgcg tgcgccactc gatgccggcg ggcttgcgaa
    25021 agccgatcca gcccacgcag cagcccaggg cgatgtcggc gagcgtgtag cgctcgccct
    25081 ggcaccaggg tctgtcgccc aggtcggcct gcatctgcgc caggccgcgg ccgatgcgcg
    25141 ccgcctgctt gccggcccag gcggcgctct gctcgggctg cggacgcagc gattcgtagc
    25201 gcaccagcag gcccgcgtcc agcacgccgt cggcgagcgc ttcccagcgg cgcaccgcga
    25261 ggcgctcgcg gttctcctcc gggatcaggc gcgagatggg cgaggcgttg tcgaggaatt
    25321 cgacgatcac gcgcgagtcg tacagcgccg tgccgtcgtc caggaccagc gtggggatct
    25381 tgcccagggg gttgtgctgg ttcaccggat tgtcggccgg ctgcacgtcc acccgctcga
    25441 agtcgcactc gatgcgtttc tcggcgagca cgatgcgcac cttgcgcgtg taggggctgg
    25501 caggagcccc gagcagtttc atggtcatgg gggcgcggat tatagcgccc gcaggcgcct
    25561 gcgcagcgcg ggcagaattt ctttcacaaa ccacggatgg cgctccagcc agagccggtt
    25621 gcgcgggctg ggatggggca ggggaaagta ctccggcagg tactcgcgcc aggcgcgcac
    25681 cgtcagcgcc agcgtcggct tgcggcggcg gccgaggaaa taggcctgcg cgtgcatgcc
    25741 gatgagcagc gtgagccgga tgccgggcag ggcggcgcgg aagcgcggct gccacagcgg
    25801 cgcgcactcc gggcgcggcg gcagatcgcc gcgctcgccg ctgcccggat agcacaggcc
    25861 cgtcgggacg atggccacgc gccgcgtgtc gtagaactgc gcccggtcca ggcgcagcca
    25921 gtcgcgcagc cgctcgccgg aggcatcgtt ccaggggatg ccggtttcgt gcacgcgccg
    25981 ccccggcgcc tgcccgacga tcatcaggcg cgcccgcgcg cccaccagaa acaccggccg
    26041 cggcccgagc ggcaggtgcg gcgcgcacac gcggcaggcg cgcgcctcct tgatcaggtt
    26101 ccgcagcatg cccatctata atagaaccta tgccttcgcc gcttggcgcg ctctcgcctc
    26161 tcgatggccg gtacgagaaa tcggcagacc cgctcaggcc ctacttcagc gaagccgcgc
    26221 tcatccgcca ccgcgtgctg gtggaactgg aatggctcaa ggcgctcgcc gccgagcggc
    26281 acatccgcga gctgccggcc ttttcgcgcc gcaccgtggc cgcgctcgac cggctggtgg
    26341 ctggtttcgg cgaccaggac gccacgcaca tcaagaacat cgaagccgag accaaccacg
    26401 acgtcaaggc gatcgagtac tggctgaagg gccggctggc gaagaacgcc gaggtgcagc
    26461 gctcgctgga attcatccac ttcgcctgca cctccgagga catcaacaac ctgtcctatg
    26521 ccctgatgct gcagcatgcg cgcgaccgcg tgatgctgcc gcgcctggac gcggtgatcg
    26581 ccgcgctgcg cgcgttcgcc cgcaggcacg ccgcgcgccc catgctgtcg cgcacccacg
    26641 gccagcccgc gtcgcccacc acgctgggca aggagatggc caacttcgcg gcacgcctgc
    26701 agcgcgcgcg cgaggcgatc gcgcgcgtgg cgatcctcgg caagatcaac ggcgcggtgg
    26761 gcaactacaa cgcgcacgcc gccgcctacc cggacttccc ctgggaggcg ttctgccgcc
    26821 gtttcgtcga gcgcctgggc ctggagttca atccctacac cacgcagatc gagccgcacg
    26881 actcctatgc cgagctgttc gacgcctatg cgcgcgccaa cacggtgctg ctcggcctgg
    26941 atcgcgacct gtggggctac gtgtcgctcg gctacttccg ccagaaggcc aaggcgggcg
    27001 aggtgggctc ctccaccatg ccgcacaagg tcaacccgat cgacttcgag aattccgagg
    27061 gcaacctcgg cgtcgccaac gcgctgctgc gccatctcgc cgacaagctg ccggtgtcgc
    27121 gctggcagcg cgacctgtcg gactccactg cgctgcgcaa tgccggcacc gcgctcggcc
    27181 acacactgct cgccctccag tcctgcctgc gcggcctggc caagctggag gcggacccgg
    27241 cgcgcatggc cgaagacctg gacgccaact gggaagtgct cgcggaggcg gtgcagcagg
    27301 tgatgcgccg gcacggcgtg cccggggcct acgagaagct gaaggcgctg acccgcggca
    27361 agcgcctgga ccgccgccag ctcgcggcct tcgtcaaggc gctgccgatc ccggcggagg
    27421 cgaaaaagcg cctgctcgcg ctcacgcccg cgtcctatac gggcctcgcg agcgcgctgg
    27481 ccaagcggat ttgagactcc tcctcgccgc cctgctcgcc tgcgcggccc cgctgcacgc
    27541 gcaggagctg gtcacgctgc cgacgcggcc cggcgtcacc ctgtcgttct tcatcgccaa
    27601 catgggcaag gtgcagccgc gcgcggcggc gctgatgctg atcggaggcg gcggcaatat
    27661 ccggctgcgc atggaaggcg gcaaggtgca cttcggccag cagaacttcc tgccgcgcac
    27721 ccggcgcgag ttcatcgccc agggcatcct gccggtgatc ctcgacaacc ccaccgacca
    27781 gcaggccggc gacggcatga cggacgattt ccgcgccggc gccaagcaca ccgaagacct
    27841 gcgcgcggtg ctggcggaag tgaagcggcg ccatccgggg ctgccggtgt tcctcgtcgg
    27901 caccagccgc agcacgcttt ccgccgccta ccaggcgcgc gcgctgggcg acgcgctggc
    27961 gggcgccgtg ctctcgtcct cgatgttccg cacgcgcgag gtacccgtgc tggcggtctt
    28021 cgatttcgcc agcatccgca tcccgttcct tttcgtgcac caccgcgcgg acgcgtgcca
    28081 cgcgaccccg tactacgacg cggagcggct gggcaggaaa tacccgctgg tgtcggtgag
    28141 cggcggcaag ccgcccgaga ccggcccctg cgatccgctg tcggcgcacg gctacttcgg
    28201 caaggaggcc gagaccgtgg cggccatcgc cgcctggatg ctgggcaggc ctttcgacaa
    28261 agaaatcaac taggcggcgc atgctgcacg ccgccgtcat tgcgctgtct tcgttcctgc
    28321 tgttcctggt gcagccgctg atcgcgcggc tgatcctgcc ctggttcggc ggcggcgcgg
    28381 cagtgtggac cacctgcatg ctgttcttcc agagcctgct gctcgcgggc tatgcctacg
    28441 cgcacgcgtc gagcgccagg ctggctcccc ggccgcaggc gatgctgcac atcgcgctgc
    28501 tcgcggcggc ggcggcgacg ctgcccatcg caccgtccga gcgctggcaa cccgagggcc
    28561 acgaggagcc cgtcaccgcc atcctgctgc tgctcggcgc cgcggtcggg ctgccctacc
    28621 tgctgctctc ggcgaccagc ccactggtgc aggcctggtt cgcgcgcgcg cgccccgggg
    28681 ccaatcccta ccgcctgttc gcgctgtcca atgtggcatc gctcgcggcg ctggtcggct
    28741 atccgctggc ggtggagccg ctgttgagca acggcgaaca ggtggcgggc tggtcctggc
    28801 tgtttgcgct gttcgccctg ctgtgcgcgg cgctcgcctg gcgcagcgcc gccccggcgg
    28861 cgcccggcgc cgcggtgctg gccgaggcgc cggcgcccgg gcgcagggac ctgctgcact
    28921 ggctggcgct gtcggcgacc ggctcggtgg tgctgctggc ggtcaccaac cacgtgacgc
    28981 agaacatcgc cgcgatcccg ctgctgtggc tggcgccgct gacgctgtac ctgctcagct
    29041 tcatcctcgc cttcgagggg cgcctgtatc gctccgaaat gttctgggcc ccgtgcctcg
    29101 ccgctttgct gggcatggcc tggctgctgg tggacgccga tttccagttc gacctgccgg
    29161 tgcagctggg cgtgttcctc gcggggctgt tcgtcacctg cctgttctgc cacggcgagc
    29221 tgtaccggct gcggccggcg ccgcggcacc tgaccgcgtt ctacctcacg gtgtcggcgg
    29281 gcggcgcgct cggcgggctg ctggtggcgg tggtcgcgcc gctcgccttc aacggctact
    29341 acgagctcgg cgtggcgatg atcgccgtgg ccttcctcgc cgcgctgcgc tgcgcgagcc
    29401 tgcaccgcgt cgcgcgcttc gcgagcctcg cggtgctgat ggcggtgagc ggcgccgcga
    29461 tctacgacgg cctgcgcttt gcgcaggacg tgcgcctcgc ttcgcgcaac ttctatggcg
    29521 tgctgcgcgt caaggagtac ggcacgcccg gcgacaccgg ccagctgcgg cggctgatgc
    29581 acggcgtgat catgcacggc gagcagtacc agcatgccga ccgccgccgc caagcgacct
    29641 cctactacca gcacagctcg ggaatcggcg cggcgctgct cgcgcgccag gcgcgccccg
    29701 ggccgatgcg cgtcggcgtg atcggcctgg gcatcggcac gctcgccgcc tacggccgcc
    29761 agggcgacac ctaccgcttc tacgagatca atccgcgcgt catgcaggtt gccgtgggcg
    29821 ctttcaccta cctgaaggac agcgaggcgc gcatcgagat gaagctcggc gacgcgcgcc
    29881 tgtccctcgg ccgggaggcg ccgcaggagt tcgacgtgct ggcggtggat gccttctcca
    29941 gcgacgcgat cccggtgcac ctgattaccc gggaggcgct cgcgctctac ctgcgccacg
    30001 tgaaggcggg cggcatcgtc gccttccacg tctccaaccg ctttctcgac ctggtgccgg
    30061 tggtggcgcg cctcgccgcc gagaacggcg tgcacgccgc ggtgctttac gaggaggggg
    30121 aagagggcga agcgcgcacg ctgagcgact gggtgctggt gtcgcgcgac cccgcggccc
    30181 tgaaggggga ggacctcgag ctggcggggg tcgtgccggc cgaggatcgc gccgcgctgc
    30241 gcacctggac cgacgactac tccaacctgg tgcagctgct caagtaacta gaccaccgcg
    30301 ccttcgttgc ggtccttgtc ggggttcttc gccggcctga tgaactgcgc gcggtccacg
    30361 cccagccaca tcaccagcgg gctggccacc agcaccgagg aatagatgcc gaacaggatg
    30421 ccgatggtga gcgccagcgc gaagtagtgc agggcgggtc cgccgaacac cagcatggtg
    30481 gtgaccatca tctgcgtgca gccgtgggtg atgatggtgc gcgagatcac ggcggtgatg
    30541 gcgctgtcga tgacctccgg cacgctcgct ttcctcatct tgcgcaggtt ctcgcgcacc
    30601 cggtcgaaca ccaccaccga ttcgttcacc gagtagccga gcaccgccag caccgccgcc
    30661 agcaccggca gcgagaactc ccactggaag taggcgaagc agcccaggat gatcaccacg
    30721 tcgtgcaggt tggcgatgat cgccgacagg ccgaagcgcc actcgaagcg catccacagg
    30781 tacagcacga tgcccaccgc ggtcaccagc agggcgaggg cgccgtactc ggcgagctcc
    30841 tcgcccacct gcgggccgac gtactccacg cggcgcagtt cgaccggctt gccctccgcc
    30901 tgcagcagcc ccatcacctt ctgcgacagg tcggcgctgg tctggccttt ctccgggtgc
    30961 atgcggatca gcacgtcgcg cgaggagccg aagttctgca cttccgcctt gaagccgccc
    31021 tggtccaggg cccggcggat gcggtccacg tcggcggact cctggtagct cagctccagc
    31081 agcgtgccgc cggtgaactc gacggagaag ttgagcccct tgctggcgag gaagaacacc
    31141 gccagcagga aggtgatcaa ggaaatgacg ttgaacacca gcgcgtggcg catgaacggg
    31201 atgacgcggc ggatcttgaa gaactccatg gctagctcac ttccacgccg tgtcgccgat
    31261 gtgcagccgt tccaccttgc ggcggtggcc gtacgcgagg ttgacgatgg cgcgcgacac
    31321 caccaccgag gagaagatcg aggtcaggat gcccaggcag tgcaccaccg cgaagccgcg
    31381 caccggcccg gagccgaaga tcagcagcga cagccccgcg atcagcgtgg tgatattgga
    31441 gtcgaggatc gtgccccagg cgcgctcgta gcccgccgcg atcgccgcct gcggcgtggc
    31501 gccgccgcgc agctcctcgc ggatgcgctc gttgatcagc acgttggcgt cgatcgccat
    31561 gccgagcgtc agcgcgatcg cggcgatgcc gggcaaggtg agggtggcct gcagcatcga
    31621 cagcagcgcc accaggaaca gcaggttcac gcccagcgcc agcgacgaga tcagcccgaa
    31681 caccacgtag tacagcgtca tgaaaacggc gatggcggtg aagccccagc gggtggagtt
    31741 gaagcccttc tcgatgttgt cgcggcccag gctcggcccg acggtgcgct cctcgatgat
    31801 ctccatcggc gccgccagcg accccgcgcg cagcagcagc gcgatgtcgt tcgcctcggt
    31861 ggtgctcatg cggccggaga tctgcacgcg cccgccgccg atttcgctgc ggatcacggg
    31921 cgcggtcacc acctcgccct tgcccttctc gatcagcagg atcgccatgc gcttgccgac
    31981 gttctcgcgc gtcacctcct tgaagatgcg cgccccggtg ccgtccagcg tcaggtgcac
    32041 cgccggctcg ttggtctgcg agtcgaagcc gggctgcgcg tcggtcagcc ggtcgccggt
    32101 gagcaccacc tgcttcctca ccagcagcgg cgcgccgttg cgctccacgt aatactcggt
    32161 gccgaacggc acctcgccgc gcgccgccgc cgccaggatt tccggcgcca tcttctcctc
    32221 gtccaccatg cggatctcga gggtggcggt gcggccgagg atgtccttcg ccttggcggt
    32281 gtcctgcacc ccgggcagct gcaccacgat gcggtcggcg ccctgctgct ggatcaccgg
    32341 ctcggccacg cccagctcgt tgatgcggtt gtgcagcgtg gtgatgttct gcttgagggc
    32401 gaactcctgc gtgcgcttgg ccgcttcggg cttgagcgcg gcgaccagca gcaggtcggc
    32461 gccttcctcg cgctcgctga gcaacaggtc gccgatgccg gcggcgatga tgtcgcgcgc
    32521 cttggcgcgc gtcccggcgt cgcggaagcg cacgcgcagg ctctggccct cgcgcgtcac
    32581 gccgccgtgg cgcacggtct tgtcgcgcag caggctgcgg atgtcgccgg tcaggctctc
    32641 cagccgcttg gtgagcgccg ccggcatgtc gacctgcagc aggaagtgca cgccgccgcg
    32701 cagatccagg cccaggtaca tgggcagcgc gttgatcgcc gtcagccagg cgggcgagga
    32761 ggacagcagg ttgagcgcca ccacgtagga cgcattggcc ggatcggggt tgaccgactt
    32821 ttccaccgtg tcccgcgcct tgagctggat gtcggtgtcg gcgaagcgca cgcgcacgct
    32881 gttggcgtcg agcacggcgc cgctgtagga gatggcggcc tgcgccagcg cgctctcgac
    32941 gcgctgcagc agcgccgcgt cgacgcgcac cgtggcccgc acgctcgata cctggacggc
    33001 gggcgactcg ccgaagaaat tcggcagcgt atacagcgcc gcgacggcca cgcacaaggc
    33061 gaccagcgcg tacttccagt ttgggtagcg gttcacgccg ccggcgctac ttcagcgact
    33121 tcatcgtgcc cttgggcagc agggtctgca ccgccgcgcg ctgcacgctg acctccgtgt
    33181 tgggcgccac ttcgagggtg acgtaggcgt cgcccatctc gctgatgcgg ccgaccatgc
    33241 cgccggcggt gatcacctcg tcgcccttct gcagcgcctc gaccatctgc ttgtgttcct
    33301 tggcgcgctt catctgcggg cggatcatga ggaagtacag cagcacgaac atcaggatga
    33361 tgggcagcag ccccatgaat ccggcatcgc ccgcgggggc ggcctgcgcg taggcgttgg
    33421 aaacgatcac agcggactcc ccagggaaag ggcgcgaatt atagcagccg ccggcgcgcc
    33481 tcccgcaggc gcgcggcggc cggcgcgagc cccgcgccct cgatggcggt gcgcagatcg
    33541 cgcatcagtt cctggtagta gtgcaggttg tgcagggtgt tcaggcgcgc gccgaggatc
    33601 tcgttcgcct tctgcaggtg gtgcaggtag gcgcggctga aattgcggca ggtggcgcag
    33661 gcgcattgcg catcgagcgg ctgcgggtcg tcgcgccagc gcgcgttgcg gatcttgacg
    33721 tcgccgaagc gcgtgtacag ccagccgttg cgcgcattgc gcgtcggcag cacgcagtcg
    33781 aacatgtcga cgccggcgag caccgcatcg atgaggtcct cgggcgtgcc cattcccatc
    33841 aggtagcgcg gccggtccgc cggcagccgc ggcgcggtgt gggcgaggat gcgctcgcgc
    33901 gcctcccgcg gctcgcccac cgccagtccg ccgatggcgt agccgtcgaa gccgatgtcc
    33961 tcgagcccgg cgagggactc cgcgcgcaga tcctcgtaca cgctgccctg cacgatgccg
    34021 aacagcgcgt tcggcgagcc gccgaaggcg tccttcgagc gccgcgccca gcgcaggctg
    34081 aggcgcatcg acttctccgc ctgcttggcc gtcgccggat gcggggtgca gtcgtcgaac
    34141 accatggcga tgtcggagtc gagcacgcgc tgcacgcgca tcgactcctc gggggtgagc
    34201 agcagccggt cgccgttgat gggcgaggcg aacttcacgc cctcctcggt gatcttgcgc
    34261 agggcgccga ggctgaacac ctggaagccg cccgagtcgg tgaggatcgg gcgctcccat
    34321 cccatgaagc ggtgcaagcc gccgtggcgc gcgatgacct cggtgccggg gcgcagccag
    34381 aggtggaaag tgttgccgag gacgatctgc gtcccgccct ccagcaactc ggcgggagac
    34441 atcgccttca ccgtgccgta ggtgcccacc ggcatgaagg cgggcgtctc cacggcgccg
    34501 tgcgcaagtt cgagccgccc gcgccgcgca gcgccgtcgc ggtggctgac ggtgaacttc
    34561 acgcggccct ctcgatcagc atggcgtcgc cgtaggagaa gaagcggtag cgcgcggcga
    34621 tggcatgcgc ataggcgcgg cggatgttct ccatgccggc gaatgccgcc accagcatca
    34681 gcaggctcga cttcggcagg tggaagttgg tcagcagccg gtccaccagg aggaactgga
    34741 accccgggcg cacgaacagc ccggtctcgc cgcgcagctg cccggtgagt gcggcgctct
    34801 ccagcgcgcg cagcgaagtg gtgcccaccg ccagcacgcg ccgcccggcg atcgcgtcca
    34861 tggtcgcgcg cgggatgtca tagcgctcct ggtgcatgcg gtgcgcgtcg atgtcctcgc
    34921 tgcgcaccgg ctggaaggtg ccggcgccca cgtgcagcgt gagcggcgcg atgcgcgcgc
    34981 cgcgcgcctc gagctgcgcg agcaggccgc ggtcgaaatg cagccccgcg gtgggggcgg
    35041 ccaccgcccc cggctggcgc gcgtacaccg tctggtagcg ctccgcgtcc tcggcgccgg
    35101 gggcgtggcg gatgtagggc ggcagcggta ccgcgccgca gcgctcgagc accgcgtcca
    35161 cgtcctcctc gaaccgcacg cggtacaggt cgtccacgcg gcccgccacc ctcacgcgca
    35221 cgccgtcgcc caccagcagc tcgccgccct cgcgcggatg gtggctcgag cggatcagcg
    35281 ccagcgcctc gcgcgcgccg gtgacgcgct cgacgaacag ctcgatcctg ccgccgctcg
    35341 ccttggcgcc ggctaggcgc gccttgatca cgcgcgtgtc gttcagcacc agcgtgtcgc
    35401 gccggccgac cagccccggc aggtcggcga aggccaggtc ctcgatgcgc ccgctggacg
    35461 cgtcgaggcg cagcaggcgg ctcgaggtgc gcgcgcccgc cgggcgctgg gcgatcagct
    35521 cctcgggcag cgcatagtcg aactccgcga ggcgcatgcc gcggcattat agggttctat
    35581 aattccgctc ccccgcgccg gaatggcgga atcggtagac gcagcggact caaaatccgc
    35641 cggccgcaag gtcatgagag ttcgagtctc tcttccggca ccagtcgtgg cgcacatcac
    35701 gttttcttgc ttttgcgtca gccgcttaac tggacaatgg gagcagcgcg gggtaggcta
    35761 gcccggtcag ttttttccag ggaagtgcat ggcgaaagcg cagcttctcg tgctgccggc
    35821 ggcgcgcaac acggcgatgg tgatcgacga ccagtcgacc ggccgcgcga tcctcgagca
    35881 ggtggtgcgt tcgctggacg agcgcgtctc ggtcgagggc ttcgggcgtc ccgtggatgc
    35941 ggtggtgtgg gcgacgcgcc acatcgccga cctggtgctg atcgactaca tgatgccgga
    36001 gatgaacggc atcgagctcg ccacgcgcct gcgcgcgctg cccggctacg agcacgtgcc
    36061 gatcatgatg gtcaccgcgc acgacgaccg caaggtgcgc tacgacgcgc tcgacgccgg
    36121 ggtcacggat ttcctcatca agccggtgga cgcgcgcgaa tgcatggcgc gctgcaggaa
    36181 cctgctgacg ctgcgccgcc agcatctggc gctggaggac aagcgccggc tgctcgaggg
    36241 catggtggag gaggcgacgc gcgaagtgcg cgaccgcgaa cgcgagacgc tgatgcgcct
    36301 ggcgcgcgcc ggcgagttca gggacgagg
//
LOCUS       DASRIE010000212         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2832816, whole genome shotgun sequence.
ACCESSION   DASRIE010000212 DASRIE010000000
VERSION     DASRIE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832816"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="ccoS"
                     /locus_tag="VFB53_09095"
     CDS             <1..140
                     /gene="ccoS"
                     /locus_tag="VFB53_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HZN86925.1"
                     /translation="ESLYLLVPVSVGLVFLTGWLFWRALHSGQFDDLERPAHDILHDD
                     D"
     gene            299..1117
                     /locus_tag="VFB53_09100"
     CDS             299..1117
                     /locus_tag="VFB53_09100"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZN86926.1"
                     /translation="MRSAVDGVLTGVKPPAFFLLAAAWAAIAVPVWLTAYVHGYAPRG
                     PLPALFWHGHEMVFGFGLAAVAGFLPLRGVPLALLALLWLAGRVAMLVPGAALLDLAF
                     PAALIAVVARELIAGRDWRNLPMLAALALLFAGNLLFHLGFYAGLWLGIATLCMLIAL
                     PAPGEPLRFVLRLGYAWLAFGLILLGLDLPGALHAVTVGAIGTMTLAAMTRATPGHAG
                     ATLAICLLVTLAAVLRVASAFAGAQAVVLTSLAGVAWSAAFAALVLRYLIARVR"
     gene            complement(1114..2490)
                     /locus_tag="VFB53_09105"
     CDS             complement(1114..2490)
                     /locus_tag="VFB53_09105"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN86927.1"
                     /translation="MPGRRVSFATVAVTGLGTFVAAAVGVTLYVSAAQSWRSTQALIA
                     QQAEALLDALEARLETRLRPEDLARFRAEHEVTPFVLDAARRVVAHPDGGEMHGPARA
                     LAEFGDPVLARLDSPDGGAPLLRGLTRSRAVRAQVDERHFLFITREMAAGSGPPWTIG
                     IYLDPVAGGQRDAMLRVVWSIAAGLGVLVVAVALAALAGRRVGRPIEALARAARAVRK
                     GQLDQVQALPRSRIYELDEANRSFGEMLQGLRERDRIRDTLGQYVPEQVARELLAGGG
                     RLEPVEAKATVLVCDLENFAALTDALGARRTIEFLNAYFEVVVAIVERHQGVVTQFQG
                     DAILAVFNLPIAARDHGANALRAALEIVRAADAQTFAGVRARNRVGLSTGRVVAGAVG
                     SSGRLSYTVHGNAVNLAARLEQMNKDYGTRILMSGKTAERCPGFALRKIADARIRGYG
                     EAVALYTL"
     gene            2575..>2796
                     /locus_tag="VFB53_09110"
     CDS             2575..>2796
                     /locus_tag="VFB53_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN86928.1"
                     /translation="METSYNYKVVRQFALMTIVWGVVGMAVGVLIAAQLLWPELNFGV
                     PWLSYGRLRPLHTNAVIFAFGGCALFATSY"
BASE COUNT          331 a         1018 c          996 g          451 t
ORIGIN      
        1 tggaaagcct ctacctcctc gttcccgtct ccgtcggcct cgtattcctg accggctggc
       61 tgttctggcg cgcgctgcac tcggggcagt tcgacgacct cgagcgcccg gcgcacgaca
      121 tcctgcacga cgacgattga agcggaagcg gtgcgccgtc ctagtgggcc atcatggcgg
      181 ccgccatcag cgccagcatc acggcgatca tggccgcgat gtgccacggg ctgctcagga
      241 cgcgacgcaa caggacgaag cgcataggcc agcctccttt tcagggactg agcacagctt
      301 gcgctccgcc gtggacggcg tattgacagg ggtcaagccg ccggcgttct ttctgctggc
      361 ggccgcgtgg gcggcgatcg cggtgccggt ctggctgacg gcgtacgtgc acggctacgc
      421 gccgcgcggg ccgctgccgg cgctgttctg gcacggccac gagatggtgt tcggcttcgg
      481 cctggcggcc gtcgccggct tcctgccgct gcgcggcgtc ccgctcgcgc tgctcgccct
      541 gctgtggctg gcgggtcgcg tcgccatgct ggtgcccggc gcggcgctgc tcgatctcgc
      601 ctttccggcg gcactcatcg ccgtggtggc gcgcgagctg atcgccgggc gcgactggcg
      661 caacctgccg atgctcgccg cgctcgcgtt gctcttcgcc ggcaacctgc ttttccatct
      721 cggcttctac gccggcctgt ggctcggcat cgctacgctg tgcatgctga tcgcgctgcc
      781 ggcgcccggc gagccgctgc gcttcgtcct gcgtctgggc tatgcgtggc ttgctttcgg
      841 cctgattctg ctgggtctgg acctgccggg ggcgctccat gcggtgacgg tcggtgcgat
      901 cggcaccatg acgctcgccg cgatgacgcg cgcgacgccc ggccatgccg gcgcgacgct
      961 cgcgatctgc ctgctcgtca cgcttgccgc cgtgctgcgc gtggcgagtg ccttcgccgg
     1021 cgcgcaggcc gtggtgctga cttcgctcgc cggcgtcgcg tggagcgcgg ctttcgccgc
     1081 cttggtcctg cgttacctga tcgcgcgggt gcgctagagg gtgtacagcg ccacggcctc
     1141 gccgtagccg cggatgcgcg cgtcggcgat cttgcgcagc gcgaagccgg ggcagcgctc
     1201 ggcggtcttc ccggacatca ggatgcgcgt gccgtagtcc ttgttcatct gctcgaggcg
     1261 cgctgccagg ttgaccgcgt tgccgtgcac ggtgtagctc agccggccgc tcgagccgac
     1321 cgcgcccgcc accacgcgcc cggtgctcag gcccacccgg ttgcgcgcgc gcacgccggc
     1381 gaaggtctgc gcgtcggccg cgcgcacgat ctcgagcgcg gcgcgcagcg cgttggcgcc
     1441 gtggtcgcgc gcggcgatcg gcaggttgaa caccgcgagg atcgcgtcgc cctggaactg
     1501 ggtgaccacg ccctggtggc gctcgacgat cgccaccacc acctcgaaat aggcgttcag
     1561 gaactcgatc gtgcggcgcg cgccgagcgc atcggtgagc gcggcgaaat tctcgaggtc
     1621 gcacaccagc accgtcgcct tggcctcgac cggctcgagc cggccgccgc cggcgagcag
     1681 ctcgcgcgcc acctgctcgg gcacgtactg gcccagcgtg tcgcggatcc ggtcgcgctc
     1741 gcgcaggccc tgcagcattt cgccgaagga gcggttcgct tcgtcgagct cgtagatgcg
     1801 gctgcggggc agcgcctgca cttggtcgag ctgtcccttg cgcacggccc gcgcggcgcg
     1861 cgccagcgcc tcgatcggcc gtccgacgcg gcgcccggcg agcgcggcga gcgccaccgc
     1921 gaccaccagc acgccgagcc cggcggcgat cgaccacacc acgcgcagca tcgcgtcgcg
     1981 ctggccgccg gccaccgggt ccagatagat gccgatggtc cacggcgggc cgctgcccgc
     2041 cgccatctcc cgggtaatga agaggaaatg ccgctcgtcg acctgggcgc gcaccgcccg
     2101 ggagcgcgtc aggccgcgca gcaacggcgc gccgccgtcg ggcgaatcca ggcgcgcgag
     2161 caccgggtcg ccgaactcgg cgagcgcgcg cgccggcccg tgcatttctc cgccatccgg
     2221 atgcgcgacc acgcggcgcg cggcgtcgag cacgaagggc gtgacttcgt gctcggcgcg
     2281 aaagcgcgcc aggtcttccg gacgcagccg cgtctccagg cgcgcctcga gcgcgtcgag
     2341 cagggcttcg gcctgctgcg cgatcagcgc ctgggtcgag cgccagctct gggcggcgct
     2401 cacgtaaagc gtcacgccca ccgcggcggc gacgaaggtg cccagcccgg tgacggcgac
     2461 cgtggcgaag gaaacgcggc ggcccggcat atcggcgact gtcggctgcg ccggggcggt
     2521 gcggcttgac ctaggtcaaa cggcacgacg gccggtttcc taggatggcg tttcatggaa
     2581 acttcctaca actacaaggt cgtgcgccag ttcgcgctca tgaccatcgt ctggggcgtg
     2641 gtcgggatgg cggtcggcgt gctgatcgcc gcgcagctgc tctggcccga gctcaatttc
     2701 ggcgtgccct ggctgtcgta cggccggctg cgcccgctgc acaccaacgc ggtgatcttc
     2761 gccttcggcg gctgcgcgct gttcgccacc tcctac
//
LOCUS       DASRIE010000213         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_224780, whole genome shotgun sequence.
ACCESSION   DASRIE010000213 DASRIE010000000
VERSION     DASRIE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224780"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFB53_09115"
     CDS             <1..392
                     /locus_tag="VFB53_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN86929.1"
                     /translation="GIATKNSILLVEYAIMARREHGLSRLEALLDACHKRARPIVMTT
                     IAMAAGMTPIAIGLGTDPSFRSPMAIVVIGGLITSTFLSLLVIPVIFTFVDDAVHKIQ
                     GFVQRHLHHAPAAAGGGAVDRGAADRD"
     gene            426..1184
                     /locus_tag="VFB53_09120"
     CDS             426..1184
                     /locus_tag="VFB53_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN86930.1"
                     /translation="MRYLPGKFVWFEHAGGDVAKARAFYEPLFGWRVEASPMGDAIYH
                     TIHNGQQPIGGMRAAAAGAPPAWRSYLSVPDVDRQYRVALELGARSVLPPTDFGAFGR
                     GAALGDPAGAVVSLWRDAEGDRIDEDPALGDWYWNELIAPDARQATAFYERLGGYTVE
                     AMDLGQSEPYRVLWASGRPRAGVFQAPDPRMPPVWVPYVRVGDCDAIVDKAQQLGAPV
                     CTPPADIPRVGRFAMLLDPQGAAIAVIRGTPAQR"
     gene            complement(1324..1827)
                     /locus_tag="VFB53_09125"
     CDS             complement(1324..1827)
                     /locus_tag="VFB53_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZN86931.1"
                     /translation="MRFRTLFLFLVLALTAVFALVNWSAFTAPTTLSLVFGTVTAPLG
                     LIMLGVVFVLGAMALAYAMYVQGTALLESRRHTRELQVQRDLVDNAETSRFTELHRFV
                     GDQLRRMEQLHADTRNQLLNRMDQLEQHTHLALHDTPRHTTVVEREETIERRVANGNA
                     GEPPHRH"
     gene            2022..3047
                     /locus_tag="VFB53_09130"
     CDS             2022..3047
                     /locus_tag="VFB53_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86932.1"
                     /translation="MAVLAACAFVVKRQTAKAEREFPPKGRFVSVDGVRLHFTVHGRD
                     DAAQTVVLLHGNGTMAEEFDISGLVRQIAERYRVVVFDRPGYGYSERPHRRRWGPEEQ
                     ADLIHAALARMGVVDPVVLGHSWGALVALAMGLRHPQDIGSLVLASGYYFPTVRMDVP
                     VLSWPALPGLGALMRHTVSPLLGRLLWPLHVRRMFSPASATPAFRERYPVGMSLRPEQ
                     LRASAQESAMMIPAAAALRHRYADLDVPAVLVAGAKDRLLSTRWHSRRLHEQLERSWL
                     RVVEDTGHMVHHVATGQVVAAIDQAAGMVWDRSLLLRPPAGLKVGEPPRVAVPPPVVP
                     CARAPTA"
BASE COUNT          392 a         1103 c         1126 g          433 t
ORIGIN      
        1 tgggcatcgc gaccaagaac tcgatcctgc tcgtcgagta cgccatcatg gcgcggcgcg
       61 agcacggcct gtcgcgcctg gaggcgctgc tcgacgcctg ccacaagcgc gcccgcccca
      121 tcgtgatgac caccatcgcg atggccgccg gcatgacgcc catcgccatc gggctcggca
      181 ccgaccccag cttccgctcg ccgatggcca tcgtcgtcat cggcggcctg atcacgtcca
      241 ccttcctcag cctgctggtg atcccggtca tcttcacctt cgtcgacgac gcggtgcaca
      301 agatccaggg cttcgtccag cggcacctgc accacgcgcc ggccgccgcg ggtgggggtg
      361 cggtggatcg cggcgcggcc gaccgggact gaggggactg aggggactga gaaatgggat
      421 cggcgatgcg ctacctgccc ggaaagttcg tctggttcga gcatgcgggc ggcgacgtcg
      481 cgaaggcgcg cgcgttctac gagccgctgt tcggctggcg cgtggaggcg tcgccgatgg
      541 gcgacgccat ctaccacacg atccacaacg gacagcagcc gatcggcggc atgcgcgccg
      601 ctgccgccgg cgcgccgccc gcgtggaggt cgtacctctc ggtgccggac gtggaccggc
      661 agtaccgggt ggcgctcgag ctgggcgcgc gcagcgtgct gccgcccacc gacttcggcg
      721 ccttcggccg cggcgccgcc ctcggggacc cggccggcgc ggtcgtcagc ctgtggcgcg
      781 acgccgaagg cgaccgcatc gacgaggatc ctgcgctggg cgactggtac tggaacgagc
      841 tgatcgcgcc cgacgcgcgc caggcgacgg cgttctacga gcggctgggc ggctacaccg
      901 tggaggcgat ggacctgggg cagtccgagc cgtaccgggt gctgtgggcc agcggccggc
      961 cgcgcgcggg cgtgttccag gcgcccgacc cgcgcatgcc gccggtgtgg gtgccctacg
     1021 tgcgcgtcgg cgactgcgac gccatcgtgg acaaggcgca gcagctcgga gccccggtgt
     1081 gcacgccgcc cgccgacatc ccgcgggtgg ggcgcttcgc gatgctgctc gatccgcaag
     1141 gggcggcgat cgcggtcatc cggggcacgc ccgcgcagcg ctgataccgg cgcgtcgcgc
     1201 tgtaacggct acagggcgac gctccgaatt acaccgtccg gcgcgcacgc ggccggggca
     1261 cgcggcgcat cccgcccgac cggcccggca tgatcggccg cgcgctcccc acggctgcaa
     1321 ggctcagtgc cggtgcggcg gctcgcccgc gttcccgttc gccacccgcc gctcgatcgt
     1381 ctcctcgcgc tccaccacgg tggtgtggcg cggcgtgtcg tgcagcgcga ggtgggtgtg
     1441 ctgctcgagc tggtccatgc ggttcagcag ctggttgcgc gtgtcggcgt gcagttgctc
     1501 catgcggcgc agctggtcgc ccacgaaacg gtgcaattcg gtgaagcgcg aggtctcggc
     1561 gttgtcgacc aggtcgcgct gcacctgcag ctcgcgcgtg tggcggcgcg attccagcag
     1621 cgccgtgccc tgcacgtaca tggcgtaggc gagcgccatc gcgcccagca cgaacaccac
     1681 gcccagcatg atcaggccga gcggcgcggt caccgtgccg aacacgagcg acagcgtggt
     1741 gggcgccgtg aaggccgacc agttcaccag cgcgaacacc gcggtgaggg ccagcacgag
     1801 aaagagaaag agggtgcgaa agcgcatgag ggactccttg cagtcgaccg ctgctcgcgc
     1861 agcaagcgac gcgcccgcgg gaacgcggct tgccgcccga agcctgtcga tcccctgtga
     1921 cggacgcttc gatgcacctg ctttcgcctt ccctgcctgc gcgccgggcg cgcgcttccc
     1981 tgcccccacc ggcgcgctgg gccgccgcag ccgccggcgc cgtggccgtg ctcgcggcgt
     2041 gcgcgttcgt cgtcaagcgg cagacggcca aggcggagcg cgagttcccg cccaagggcc
     2101 gcttcgtcag cgtcgacggc gttcggctgc acttcaccgt gcatggccgc gacgacgccg
     2161 cgcagacggt cgtgctgctg cacggcaacg gcaccatggc cgaggaattc gacatcagcg
     2221 ggctggtgcg ccagatcgcc gagcgttatc gcgtggtggt gttcgaccgc cccggctacg
     2281 gctacagcga gcggccgcac cggcgccgct ggggccccga ggaacaggcg gacctgatcc
     2341 acgccgcgct cgcgcgcatg ggcgtggtcg atcccgtggt gctgggccat tcgtggggcg
     2401 cgctggtcgc gctcgccatg ggcctgcgcc atccgcagga catcggcagc ctggtgctcg
     2461 cctcgggcta ctacttcccg accgtgcgca tggacgtgcc ggtgctgtcg tggccggcgc
     2521 tgccggggct cggcgcgctg atgcgccaca ccgtgtcgcc gctgctgggg cgcctgctgt
     2581 ggccgctgca cgtgcgccgc atgttctcgc cggcgagcgc gacgccggcg ttccgcgagc
     2641 gctatccggt cggcatgtcg ctgcggccgg agcagctgcg cgcgagcgcc caggagagcg
     2701 cgatgatgat cccggccgcc gccgcgctgc gccaccgcta cgccgatctc gacgtgcccg
     2761 ccgtgctggt ggcgggggcg aaggatcgcc tgctcagcac gcgctggcat tcgcgccggc
     2821 tgcacgagca gctggagcgc agctggctgc gcgtggtcga ggacaccggc cacatggtcc
     2881 accacgtggc cacgggccag gtggtcgcgg cgatcgacca ggccgccggc atggtgtggg
     2941 accgatcgct gctgctgcgg ccgccggccg ggctgaaggt gggcgagccg ccccgggtgg
     3001 ccgtgccgcc gccggtggtg ccgtgcgcca gggcgcccac cgcatgactt cacg
//
LOCUS       DASRIE010000214        11423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2543519, whole genome shotgun sequence.
ACCESSION   DASRIE010000214 DASRIE010000000
VERSION     DASRIE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543519"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..783)
                     /locus_tag="VFB53_09135"
     CDS             complement(88..783)
                     /locus_tag="VFB53_09135"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZN86933.1"
                     /translation="MSAVITDTTSPTSGGPSARSARRSVAFLRWLRRIHLYVGLWGAA
                     LGLLFGITGVLMNHRAVLKIPLEKAVQGTAQLPLPDGQPPFENAEAMGRWLQAELHFA
                     NAQSERIRTEPPRRVVWADRELQQPARWTFNWQAPDRGVNAEYVVGNRFVKVDSQDAT
                     PVGTLMRLHTASGTGVFWVLLSDTIGGALTVLAITGLLLWSRLHRIRLAAVSTSLVAL
                     LAAGWFLWTAGLS"
     gene            complement(793..924)
                     /locus_tag="VFB53_09140"
     CDS             complement(793..924)
                     /locus_tag="VFB53_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86934.1"
                     /translation="MRIQTAANMQYLVLPRPIYPRASRLAGEAGRVLHNLLRMWAQP"
     gene            1029..2132
                     /locus_tag="VFB53_09145"
     CDS             1029..2132
                     /locus_tag="VFB53_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZN86935.1"
                     /translation="MSHINALQISSAKAGPALTPAQKRFNTLIRQIEQARQSLAAWNE
                     HIAAYRQAHAQVLLPLQGELIAGRRQWAFALDALLDQRHWTKAERGTLRELLCESAGE
                     LLEARGEDAELKALFDKHAEVDFDTEQREAVQAMKDLTEAMTGLDLGADEGIETEADL
                     FERMQQGLRERAATEEAQRSERAARRRKSAAQQRREAEAQEATHSVREIYRKLASALH
                     PDRETDEQQREAKTSLMQRVNQAYGANDLLALLELQLQIEQIDASHIANASAQRLKHY
                     NKVLSEQLSELKAEMDHVEMGFRMEFGLEPGWGMNPRKLGEVLERTRRQWRAELSEQQ
                     REMRMLGDVAATKRWLKRQRQLLREAEFDFAPF"
     gene            complement(2156..2353)
                     /locus_tag="VFB53_09150"
     CDS             complement(2156..2353)
                     /locus_tag="VFB53_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86936.1"
                     /translation="MTSRDVTPAPLPAHWTPEQALAVFECLQALREQLWEMYGPEVQQ
                     AWRDQLEPQQPLPEFDPDQPF"
     gene            complement(2350..3138)
                     /gene="istB"
                     /locus_tag="VFB53_09155"
     CDS             complement(2350..3138)
                     /gene="istB"
                     /locus_tag="VFB53_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZN86937.1"
                     /translation="MSETTMNPTELRARATDLRLHGLLAHWSEVMAEEEAARRVAQWL
                     AWETHERGRRSLERRLREAHIGRFKPLADFDWSWPAQVDRAAVEDLMTLQFMADATNV
                     VFVGPNGVGKSMCACNLGYQAVLAGHTVLFVTAGHLLGDLAALDSDSTLRRRLRHYAA
                     PDVLLIDEVGYLSYSNRHADLLFELVSRRYQHKSTVFTTNKSFADWGEVFPNAACVVS
                     LVDRLMHRAEVVRIDGESYRAKEAQERLAARAKQRSGKSGRSKP"
     gene            complement(3131..4648)
                     /gene="istA"
                     /locus_tag="VFB53_09160"
     CDS             complement(3131..4648)
                     /gene="istA"
                     /locus_tag="VFB53_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZN86938.1"
                     /translation="MVIPADLSARILRLHEVEHWRVGTIARQLHVHRDTVRRVLAQAG
                     SPAPATPLRPSRVDPYRAFILETLTKFPTLTAARLFVMVHERGYRGSPEHFRHLVAGM
                     RPRRAAEAYLRLRTLPGEQAQVDWAHFGHLQIGRARRPLMAFLMVLSYSRLIFLRFFM
                     DARMDSFLRGHVEAFSAWDGCPRVILYDNLRSVVLERIGDAVRFNPELLRFAAHHFFE
                     PRPVAVARGNEKGRVERSIRYVRDAFFAARKFSDLDDLNAQARAWCQGQASDRRWPED
                     GTLSVRDAFATERLSLLALPQREYLLGERRTVDVGKTPYVRFDLNDYTVPHTHVRRTL
                     SVLADERRVRVFDGVQEVASHVRSYDSGQQVEELAHIQALVEHKRQARAHRGVDRLAH
                     AAPASATLLQRAAERGHNLGTVTAALLRLLDRYGAAALQAAIDRALAQGVPHPNAVRL
                     ALEHAREEQGLPPPTALGLSEEVARRDAPVRPHALASYDQIVQAAQPASGDDDDE"
     gene            complement(5563..5733)
                     /locus_tag="VFB53_09165"
     CDS             complement(5563..5733)
                     /locus_tag="VFB53_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86939.1"
                     /translation="MTVPAPPSVQAPAPAAPVADAALLLEVPAPVAGAAPVIPPSGVQ
                     CERRSGSALIRR"
     gene            5956..6669
                     /locus_tag="VFB53_09170"
     CDS             5956..6669
                     /locus_tag="VFB53_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZN86940.1"
                     /translation="MNAPPDSGSPPDTTAEFDVLDRLLQRRHSCRGFLPRPVPRQVIE
                     RILTTAQRTASWCNAQPWQVHIVSGETLEALRIELPAGARSGKPAAPEVDWPREYRGI
                     YLTRRRECGWNLYRATGVQKGDREGAARQALENFTFFGAPHVAIVSSDEALGTHGVMD
                     TGAWVSNFMLAAAALGVASIAQAALASYPDILREHLEIAADRRIVCGISFGFEDTHHP
                     ANGFRTSRAPLDEAVRWTG"
     gene            complement(7692..9698)
                     /locus_tag="VFB53_09175"
                     /pseudo
     CDS             complement(7692..9698)
                     /locus_tag="VFB53_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9804..10505)
                     /locus_tag="VFB53_09180"
     CDS             complement(9804..10505)
                     /locus_tag="VFB53_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HZN86941.1"
                     /translation="MSTHIASPSRHAQPPLAPVEQSRADFYALIASLLRAPPDAGLLE
                     RLGDAHTVSSPDASPFERAWLDLIGAARRADPAGVAAEHARLFAAPAGQAAAVDSSAA
                     RDARGHRRGEPRATLPANRHDPGLARHACRAGADDPLGSLCEAMRLLIAGAPGVPRQG
                     IARQRLFFDTRLAGAVPACLDDLRQAQAGGFHARLADFVEAFFAVEAHAFAIDDAPPC
                     RRPAPALAHAAEAAA"
     gene            10746..>11423
                     /locus_tag="VFB53_09185"
     CDS             10746..>11423
                     /locus_tag="VFB53_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86942.1"
                     /translation="MRAPMATAFWIVVAAALVLAALFLKQDRLLYFPARAPVEQVASE
                     ALRPWPGPADFRGLLGEPAGAPRGTAVVFHGNAGHAGHRGYYAQALGSLGWRVVLAEY
                     PGYGPRAGIPGEAGFVADALETLDLAHRQFGAPLLVVGESLGAAVAAQAAASRQDIIA
                     GLLLVTPWDRLAHVGAHHYPWLPVGWMLRDPYDSARHLARVRRPVVIAVAEHDRIVPA
                     RFGRALHD"
BASE COUNT         1727 a         3997 c         4252 g         1447 t
ORIGIN      
        1 acctcccctt cgacaagctc aggacaggcc cttcgacaag ctcaggacag gcccttcgac
       61 aagctcagga caggcccttc gacaagctca ggacaggccc gctgtccaga gaaaccagcc
      121 ggccgccagc agcgcaacca ggctggtgga aaccgcggcg agccgaatgc ggtgcaggcg
      181 cgaccacagc agcaagccgg tgatggcgag caccgtcagc gcgccgccga tggtgtccga
      241 cagcagcacc cagaacacgc cggtgccgct ggcggtatgc agccgcatca gcgtgccgac
      301 cggcgtggcg tcctgcgaat cgaccttgac gaagcggttg cccaccacgt attcggcgtt
      361 gacgccacgg tcgggcgcct gccagttgaa ggtccagcgt gcgggctgct gcagctcgcg
      421 gtcggcccac accacgcgcc gcggcggctc cgtcctgatg cgctcgctct gcgcgttggc
      481 gaagtgcagc tcggcctgca gccagcggcc catggcctcg gcattttcga aaggcggctg
      541 gccgtccggc agcggcagct gggccgttcc ctgcacggcc ttctcgagcg ggatcttcag
      601 caccgcacgg tgattcatca gcacgccggt gattccgaac agcaagccga gcgccgcccc
      661 ccacagcccg acgtagagat ggatgcgccg cagccagcgc aggaaggcga cgctgcgccg
      721 tgcgctgcgc gcggacggac cgccggacgt cggggaagtc gtgtcggtga tgacggcgct
      781 cattcggagt cctcaaggct gcgcccacat gcgcagcagg ttgtgcagca cgcgcccggc
      841 ctcgccggcg aggcgcgacg ctcgcggata gatcggccgg ggcagcacca ggtactgcat
      901 gttggcggcg gtctggatcc gcatctattc ggtggtgagg aggcggtttg ggtgtgtggg
      961 cgggccggcg ggtgctgagg gccagctatg aacgacaatc gcgggccatc gcagcttgag
     1021 gtggtcccat gtcgcacatc aacgccctgc agatttcgtc cgccaaggcc ggcccggcat
     1081 tgacgccggc acagaagcgc ttcaacacgc tcatccgaca gatcgagcaa gcaaggcaga
     1141 gtctggccgc gtggaacgag cacatcgctg cataccggca agcgcacgcc caggtgctgc
     1201 tgccgctgca gggcgagctg attgcgggac gcaggcaatg ggcgttcgcg ctcgatgcgc
     1261 tgctcgacca gcggcactgg accaaggcgg agcgcggcac gctacgcgag ctgttgtgcg
     1321 agtcggctgg ggagttgctc gaagcgcgcg gcgaggacgc cgaattgaag gcgctgttcg
     1381 acaagcacgc cgaggtcgac ttcgacaccg agcaacgcga ggcggtgcag gccatgaagg
     1441 acctgaccga ggcgatgacc ggtctggacc tcggcgcaga tgaagggatc gaaacggagg
     1501 ccgacctgtt cgagcgcatg cagcaaggcc tgcgggagcg agcggcgacc gaggaagccc
     1561 agcgcagcga gcgggccgca cgccggcgca agagcgcggc gcagcagagg cgcgaggccg
     1621 aggcgcagga ggccacgcac tcggtgcgcg agatctatcg caagctggcc agcgcgttgc
     1681 atccggaccg ggagaccgac gagcagcaac gcgaggcgaa gacctcgctg atgcagcgcg
     1741 tcaaccaggc ctacggggcc aacgatctgc tcgcgctgct ggagctgcag ttgcagatcg
     1801 agcagatcga tgcgagccac atcgccaatg ccagcgccca gcggttgaag cactataaca
     1861 aggtgctgag cgagcagctg agcgaactca aggcggagat ggaccatgtc gagatggggt
     1921 tccgcatgga gttcggtctg gagcccggct ggggaatgaa cccgcgcaag ctgggcgagg
     1981 tgctcgagcg gacgcgccgc cagtggcgag ccgaactgag cgagcagcaa cgtgagatgc
     2041 gcatgctggg cgacgtggcg gcgacgaagc gctggctgaa gcgccagagg caactgcttc
     2101 gcgaagccga gttcgacttc gcgccgttct gaccaaggaa gagctctggc ggcgatcaga
     2161 agggctggtc gggatcgaac tcgggtagcg gctgttgcgg ctcgagctga tcgcgccaag
     2221 cctgctgcac ttcgggcccg tacatctccc acagttgctc gcgcagtgcc tgcaggcact
     2281 cgaagacggc cagtgcctgc tcgggtgtcc agtgcgccgg cagcggcgcc ggcgtgacgt
     2341 cgcgcgaggt cacggcttgc tcctgccgga cttgcccgag cgctgcttgg cgcgcgcggc
     2401 cagtcgctcc tgcgcctcct tggcgcggta cgactcgccg tcgatgcgca cgacctcggc
     2461 gcggtgcatc aggcgatcca cgagcgagac gacacacgcc gcattgggga acacctcacc
     2521 ccagtctgca aacgatttgt tcgtagtgaa caccgtgctc ttgtgctggt agcgccggct
     2581 gacgagttcg aacagcaggt cggcgtgacg gttggagtag gacaggtacc cgacttcgtc
     2641 gatgagcagg acgtctggcg cggcgtagtg gcgcagcctg cggcgcagcg tcgagtcgct
     2701 gtccagcgcg gcaaggtcgc cgagcagatg cccagcggtg acgaacagca cggtgtggcc
     2761 ggcgagcacc gcctggtagc ccaggttgca cgcgcacatc gacttgccca cgccgttggg
     2821 tccgacgaac acgacgttgg tggcgtcggc catgaactgc agcgtcatga gatcctcgac
     2881 ggcggcccgg tcgacctgcg cgggccagct ccagtcgaag tcggccagcg gcttgaagcg
     2941 cccgatgtgc gcctcgcgca ggcggcgctc caggctgcgc cggccacgct cgtgggtctc
     3001 ccaggccagc cactgcgcga cgcgccgtgc ggcttcttcc tcggccatca cctcggacca
     3061 gtgggccagc agcccgtgca gccgcaggtc agtggcccgc gcgcgcagct cggtggggtt
     3121 catcgtcgtc tcactcatcg tcgtcgtctc cggaagctgg ttgtgcggcc tgcacgatct
     3181 ggtcgtagga ggcgagtgca tgcgggcgca ccggcgcatc gcgccgcgca acctcctcgc
     3241 tcaggcccag ggcggtgggc ggcggcaggc cctgctcctc gcgtgcgtgc tcgagtgcca
     3301 ggcgcacggc gttggggtgc ggaacaccct gcgcgagcgc gcgatcgatg gccgcctgaa
     3361 gtgccgcggc gccgtagcga tcgagcaggc gcagcagcgc ggcggtgacg gtgccgaggt
     3421 tgtgaccgcg ctcggccgcg cgctgcagca gcgtcgcgct ggccggcgcg gcatgcgcga
     3481 gccggtcgac accgcgatgg gcacgggctt ggcgcttgtg ctcgaccagc gcctggatat
     3541 gggcgagctc ctcgacttgc tggccgctgt cgtagctgcg cacgtgcgag gccacttcct
     3601 ggacgccgtc gaacacacgc acgcggcgct cgtcggccag taccgacagc gtgcgccgca
     3661 cgtgcgtgtg cggcacggtg tagtcgttca gatcgaagcg cacgtacggc gtcttgccga
     3721 cgtcgacggt ccggcgctcg cccaggaggt actcacgctg cggcagggcc agcaggctga
     3781 ggcgctcggt agcgaacgca tcgcgaacac tcaaagtgcc gtcctcgggc cagcggcgat
     3841 ccgacgcctg accctggcac cacgcccgcg cctgggcgtt cagatcatcg agatcgctga
     3901 acttgcgtgc ggcgaagaag gcgtcgcgga cgtaccggat gcttctctcg acgcgcccct
     3961 tctcgttgcc gcgggcgacc gctaccgggc gcggctcgaa gaaatgatgg gcggcgaacc
     4021 tgagcagttc ggggttgaag cgcacggcgt cgccgatgcg ttcgagcacg acgctgcgaa
     4081 ggttgtcgta gagaatgacc ctcgggcaac catcccaggc gctgaaggcc tcgacgtggc
     4141 cgcgcaggaa gctgtccatg cgcgcatcca tgaagaagcg aaggaagatc agccgcgagt
     4201 agctgagcac catcaggaag gccatcagcg gtcggcgtgc gcggccgatc tgcaggtggc
     4261 cgaaatgagc ccaatcgact tgcgcctgct cccccggcag ggtgcgcagc cgcaggtacg
     4321 cctcggcggc acggcgcgga cgcataccgg cgacgaggtg ccggaagtgc tcggggctgc
     4381 cgcggtagcc gcgctcgtgc accatgacga acagccgcgc ggcggtcagc gtcgggaact
     4441 tggtcagcgt ctcgaggatg aaggcgcggt acgggtcgac acggctgggc cgcaggggcg
     4501 tggccggcgc gggcgagccg gcctgcgcga gcacgcgtcg cacggtgtcg cgatgcacgt
     4561 gcagttggcg ggcgatggta cccacgcgcc agtgctcgac ttcgtgcagg cgcaggatcc
     4621 tggcgctcag atcggcaggg atgaccaagg aacctccctc acggactggg atcgaccacg
     4681 cggggcggcc agcgtcggct gcggccgtgc cgcaggaagc cgtggcgcac ccacggcgac
     4741 agcggcgcgg cgcaacgccg gcagcgcacg gtcatcggcg gcggcgtgtt cgattcgcac
     4801 tccccgaccg tgctgctgcc gaccgaggcc agtggagcat cggccggccg catcggggaa
     4861 ccctggtgcg tcacgaagtt gatggcgccg tcgtcgaagg cggcggccgc tcggcatgct
     4921 cgctcgcgtt cacggcgccg ctggcgccag cggcgcgagc gcgctgcgtg tgccagacgg
     4981 ccggcacggc tgcgctggta gcgacgcgcg gcttcgcgcc gtgagtcccg ccgcgccgcg
     5041 ctcgaacagg cacgtccgca ataacgctgg ccgcgatcac agcggctgca cagcagcacc
     5101 tgcgcacggc aacgcgcaca cacgaacagg cgggcgggat ggtcgaccag ctgccgggct
     5161 cctcgagatc aatcccgagg acgccgccgg tcgcgagcgc ggcgccgtga cggcctgaac
     5221 ggccgtgcgc aatacactgg cgctgcatcc cgcttcccag cgcggtgtag ggcacggcgt
     5281 gcggttcttt agctgccagg cgatggccgc cgccgtgccc gcctaccact cgggcacggc
     5341 gctggttcta ccgcaaccgc agccggcact cccccagcgc atatccccca ccgggttcgc
     5401 cgccgactgg catgccgccc tgcagggcgg catgccagtc gggacctcat cgttcacgaa
     5461 cgattgagcc gaaacgccgc ctcgcagccg ctcgtgaaaa cgtcatctcg tcgccgctcg
     5521 gcgaaaacga cacctcgccg tcgcgtggac gaaaccgcca cctcaccgcc gaataagcgc
     5581 gcttcctgac cgccgctcac actgcacgcc cgacggcggg atgacgggcg cggcaccggc
     5641 caccggtgcc ggcacctcca gcagcaaggc tgcatcggcg accggcgcgg ccggcgcggg
     5701 cgcctgcacc gacggcggcg cgggcacggt catgggttcg gaccgcggcg gcgcgccggt
     5761 ggcgcctctc gaggtcggcc ttcccgagga aggcgcgccg gcgatggtgc gaccgatgtg
     5821 ccgcgggtcg actataaatg cgaatctgtc gcattggcaa ccgcattcgc tgcgaacgat
     5881 gtccgcgcgt cgccgcgcga tggcgcgtga tagaaattcc cccccgtgcc gcgacggcaa
     5941 gccaccgaga gcccgatgaa tgcccctccc gacagcggat cgcccccgga caccacggcc
     6001 gagttcgacg tcctcgaccg gctcctgcaa cggcgccaca gctgccgcgg cttcctcccc
     6061 aggcccgtcc cccggcaggt gatcgagcgg atcctcacga ccgcgcagcg caccgcctcg
     6121 tggtgcaatg cccagccgtg gcaggtgcac atcgtgagcg gcgagacgct cgaagcgctg
     6181 cgcatcgagc tgccggccgg ggcacgctcg ggcaagccag ccgcgccgga ggtggactgg
     6241 ccgcgcgaat accgcggcat ctacctgacg cggcggcgtg aatgcgggtg gaacctgtac
     6301 cgcgccaccg gggtgcagaa gggagaccgc gaaggggcgg cacggcaggc cttggagaac
     6361 ttcacgttct tcggcgcgcc gcacgtggcc atcgtgagca gcgacgaggc cctcggcacc
     6421 cacggcgtga tggacaccgg tgcgtgggtg tcgaacttca tgctggcggc cgccgcgctg
     6481 ggcgtggcgt cgatcgcgca ggccgcgctc gcgtcctatc ccgacatcct gcgcgagcac
     6541 ctcgaaatcg cagcggaccg ccgcatcgtg tgcggcatct cgttcggatt cgaggacacg
     6601 caccaccccg ccaacggctt tcgcacctca cgtgcgccgc tcgacgaagc ggtgaggtgg
     6661 acgggatgaa ccgtttgcca tcgcgaccgg cgccgcggcg ccgcgggacg gccgcggcgc
     6721 ctcgggcgcg ccgcggccgt cccgcgcgcg tcgccgtcga gatgcgcgac gaggatctgc
     6781 agcgcgaggt tgcgctgcgc gggcggcgcc accgcctcgc cgtgccgcgc ccacgcggcg
     6841 cgcgcgccgg cccggtcgcc ggccgccagg tggccggcca gcgccagccc gagcaggaag
     6901 cgcgcgcgca ccggcgtgcg tccttcgccg gcctgcagca atgcgtcggc cgatcgcgcg
     6961 agcgcgggat cgcgcgccgc cacgcgggcc agcagttcgc gccacgcgtc gcccgcggcg
     7021 cccggcatct gcaggcaggc ctgcccgcgc aggcgctcca gcagcacgcg cccctgctcg
     7081 gcggacagat acggcagcac cgtgccggcg atgcggacca tcgcgtaggg cgcgtcgccg
     7141 ttcggcggct ggcggcaggc cgcgaggaga tcggccgccg ccgcctccac gaccctcgcc
     7201 accggatgcg ccggcacgcg catgggcagc ccgagcagac gttcgaacac gcgctgcgcg
     7261 gcgagcgccg gggcctggtc gccgaagtgg gtgttgagcg tgatgtccgc cggcgtctcg
     7321 aacgacgggc gcccgcccag cagctggggc agcaggttgg gcgagagctc cccctcgatg
     7381 tgcgacgccg gccagccgcc gccgacgaag cggtcgcggt cggcgtgggt gtccaggaac
     7441 gggcggaagt cggagttggc cggcacgtcg cgcgccttca gccacggacc gagcaaggcc
     7501 ttgtcgccga tgaggcgcac cgtgaagtcg ccgttcgagc ggacctcgag ccggtcgacg
     7561 agccggcgcg tggcgggcgc gagccgctcg agcgcatcgg cgggcagcgc gagcgggccg
     7621 cggcgcttcg ccacgagcac caagtcgccg tggttggcgc cgaacagcag gtagtcgccg
     7681 aagaccgcgt ccatcgcctt gagcaccgag accacgcggt ccgggtgggt ctcgtacacc
     7741 tggatccact gcacgaacag cccgtcgtcc gcgaggtagc gctggacgtg ccggtagaac
     7801 tcgatcgaga acaggctcgc cacgccgctc acccagggat tcgacggttc cgagacgatc
     7861 atgtcgtagc gctgccggcg ggcggcgaag tacgacttgg cgtcgtcgta gtggacggcg
     7921 ctgcgcgggt cgtcgtaggc ggacgcgttc agcggccgga agtagcgcgc gagctccacc
     7981 atccggggct cgatctcgat ggagtcgacg gcggccacgc gcgggtcgga cagcagcgtg
     8041 tgcgcggtga tgcccgagcc gaagccgatg ttcgcgatgc gcgccggcgc ggcatggatc
     8101 gcgagcggca gcgcggggat gagcgccatc gtcacctcgt cggtgcggta ggcgccgccg
     8161 cggtcgacgt aggccgacgc gtcggccttg ccgttggtgc ggatcgcgat cgcgcccgcg
     8221 gcgtcgcggt ccaccgacac ggtggccgtc ttgccgtcgg ccatcgcgag cacggtgttg
     8281 tcgatgcgtg actggccgga gcggtagacg cccgaggcca gctgctgcgg atcgaacccg
     8341 gcgcggatca gcagcgcccc cgcgacgctt cccgccagcg ccacgccggc atagggcatc
     8401 gcacgcggcc gcgcgaacac gccgagcagc cacaggccga gcagcacgtc gatcgcggcg
     8461 ccgagggcga ccacgtggcg caggccgaga accggcaggc cgacgtggat ggcgaacgcc
     8521 acgccggcga tcgcgcccag cgtgttggcg ctgtagatcg cgccgatggc gcgctcgccg
     8581 tgccggcggt ggccgaacag caggcgggtc gcgagcggca gcgtcatgcc ggcgcagaac
     8641 gccgccggca tcatcaccgc ggccgagacg ccgtagcgcc cgaggttgag cagcaggtag
     8701 ccgcggtccg tgcggggcgc ggcggaaacc agccagccga tgaactcgaa gctcgccgca
     8761 tagagcggca gggtggccag cgcgcacagc cccatcacca cctgcacctg gccgagcagc
     8821 gcgccgacgt tggcgtggtc gtcgatgcgc cgtcgcaccc acagcccgcc gagcgcgagc
     8881 ccgaggatga acgccgacag catcagctcg aacgagtggg tggcgctgcc gagcaccagc
     8941 gagagcatgc ggatccagcc cacctcgtag acgaaggagg aggcgccggt gacgaaggcg
     9001 acggcgagca gcagcgcggt gaggcggtcg agcgcgaacg gggcgcggtc gtccggcgcc
     9061 ggtgtcgcgc ggcccgtgcc ggccgcggcc cacgccgaaa ccgccgcgat cgccaggttg
     9121 caggcgccgg cgagcgcgat cgtgcccggc agcccgaggg ccgggatcgc ccagaagccg
     9181 ctggcgagca cgccgatcgc gccgcccagg ctgttgacga agtacagcgt ggcgaaggtg
     9241 tggccctcgc ggccgcgcgc atgccgcgcc aggccggccg cgaagagcgg gaaggtcgcg
     9301 ccgagcagcg tggcggccgg caggatgagc gccaccgcga gcgaccattt cgcggcggcg
     9361 aacgccgccg cgcccagcgc gcccgactcg gccagccggt acagcccctc ggtgctttcg
     9421 acgaacaccg ggtggaacgc cagcgccagc acgccgatgg cgccttcggt gagcgcatag
     9481 gcgcgcagcg ggttgccgag ccgccgcagc gcccgcccgg ccgccgcggc gcccagcgcc
     9541 atgccgccca tgaagatcgc cagcacgagc gtctgcgcgt aggccgcgtg gccgaggaag
     9601 agcttgaggt aatggctcca gatcgactcg tagatcagcc ccgcgaatcc cgaggcgaaa
     9661 aagacgagcg tgaacagcga atgctggctg ggccgcatgg tcgtggactc cgtcccgttg
     9721 gccgggcgaa gtctatcggg cccgcgcgcc gggtcagccc gtcctcgcgc cgcgccgggc
     9781 gcgccgcgtc gccgccgggc gtatcacgcg gccgcttcgg ccgcgtgcgc cagggcgggc
     9841 gccggccgcc ggcagggcgg cgcatcgtcg atcgcgaacg cgtgcgcctc gaccgcgaag
     9901 aacgcttcga cgaaatcggc cagccgcgca tggaagccgc cggcctgcgc ctgccggagg
     9961 tcgtccaggc acgcggggac cgcgccggcg aggcgggtgt cgaagaacag ccgctgccgc
    10021 gcgatgccct ggcgcggcac gcccggcgcg ccggcgatca ggaggcgcat cgcctcgcac
    10081 agcgagccga gggggtcgtc ggcgccggca cggcaggcgt gccgcgcgag cccgggatcg
    10141 tgccggttcg cgggcagcgt cgcgcgcggc tcgccccgcc ggtggccgcg ggcgtcgcgc
    10201 gcggccgacg aatccaccgc cgccgcctgc ccggcggggg cggcgaagag ccgggcgtgc
    10261 tcggcggcca ccccggcggg gtcggcgcgg cgcgccgcgc cgatcaggtc cagccaggcg
    10321 cgctcgaacg gcgaggcgtc gggcgaggag acggtgtgcg cgtcgccgag ccgttccagc
    10381 aggccggcgt cgggcggcgc gcgcagcagc gaggcgatga gggcgtagaa atcggcacgc
    10441 gactgctcga cgggcgccag cggcggctgc gcgtgccggg agggggaggc gatgtgcgtc
    10501 gacatggcat gcggctcgga ctggttcgca ccgagcagcc ggcgtgcccg ccgggcaggg
    10561 cgcggccggg gccggccggc gagcggccgg ccggcggggc ccgcggccgc cggcgcgcgt
    10621 cccgcgcccc tcgcgtcggt aactttggcg ccgtgtgtgt gcaagggatg gacgcacgcc
    10681 ctggcgcgcg ccgccgggcc gcggcacgat gcgggcgccc gcgctcgcgc gcacctgccc
    10741 gacccatgag agcaccgatg gccaccgcgt tctggatcgt cgtcgccgcc gcgctggtgc
    10801 tggcggcgct tttcctgaag caggaccgcc tgctctactt tccggcgcgc gcgccggtgg
    10861 agcaggtggc ctccgaggcg ctgcggccgt ggcccgggcc ggcggacttt cgcgggctgc
    10921 tcggcgaacc cgcgggcgca ccgcgcggca cggcggtggt cttccacggc aacgccggcc
    10981 atgcgggcca tcgcggctac tacgcgcagg cgctcgggtc gctcggctgg cgggtggtgc
    11041 tcgccgaata cccgggctac gggccgcgcg cgggcatccc cggggaggcc ggcttcgtcg
    11101 ccgacgcgct cgagacgctg gacctcgcgc accggcagtt cggcgcgccg ctgctcgtcg
    11161 tcggcgagtc gctgggtgcc gcggtggccg cgcaggccgc ggcgtcgcgc caggacatca
    11221 tcgccgggtt gctgctcgtc accccgtggg accggctggc gcacgtcggc gcgcaccact
    11281 acccctggct gccggtgggc tggatgctgc gcgaccccta cgacagcgcc aggcacctcg
    11341 cgcgcgtgcg gcgaccggtg gtgatcgcgg tggcggagca cgaccgcatc gtgcccgcgc
    11401 gcttcggccg cgcattgcac gac
//
LOCUS       DASRIE010000215         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1268335, whole genome shotgun sequence.
ACCESSION   DASRIE010000215 DASRIE010000000
VERSION     DASRIE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268335"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFB53_09190"
     CDS             complement(<1..191)
                     /locus_tag="VFB53_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86943.1"
                     /translation="MALPERQTAGRRPEIAPALFRLLAEAALSRAALDACGLPVALAD
                     AADASRPLSYVNQAFEAFFG"
     gene            complement(293..1240)
                     /locus_tag="VFB53_09195"
     CDS             complement(293..1240)
                     /locus_tag="VFB53_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86944.1"
                     /translation="MNERLNQNDVTNRINVEDPDQVRDAVLALFTARYPGADLAPLRR
                     AFDDVKSLFGGTYPGYLPCDTLYHDLRHTLDMTLAMARMLDGHDRSRAPAEQVGARRA
                     MLGVVVALLHDSGYLKRASESHIENGAVFTKVHVSRSADFLARYLPQVGFAAEAGAAS
                     KLVHFTGYEMDIDDIMLHDPKDRLLGCLVGTADLIGQMSDRMYLEKCREFLYTEFVWG
                     HIARETLADGREIVRYASPEDLMLKTPGFYEYVARARIEKKLGGVDRFAEAHFDGPSL
                     YQTEIDRNMHFLKDAIEQADLKRLRRFCYSLSRKDAKAA"
     gene            complement(1439..1770)
                     /gene="rnpB"
                     /locus_tag="VFB53_09200"
     ncRNA           complement(1439..1770)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB53_09200"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1870..2382
                     /locus_tag="VFB53_09205"
     CDS             1870..2382
                     /locus_tag="VFB53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HZN86945.1"
                     /translation="MRRRLYFLLPDVDSARRTANDLLLARIEDRHMHFLARRGTQLGE
                     LPEASYLQKSDLVHGAGIGFALGGLGGLLLGALIVAFPIEGTQPQLVAVPVAAVVGAL
                     LGAWMASMAAAAVPNSRLEAFQDAIAGGKVLLMLDVPLARVEEARDLVRTQHPEALSG
                     GMETRYPAFP"
     gene            complement(2342..2956)
                     /locus_tag="VFB53_09210"
     CDS             complement(2342..2956)
                     /locus_tag="VFB53_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HZN86946.1"
                     /translation="MPPAGGGPTYEMQLFETGQVQTRAGNRHDLFNALCWLAFPRTKA
                     RVNALHAAEIPREGGKRGRLRDLLTIFDEGGALVLCADEGLNALVRECRWKELFWDRR
                     ERVRAAMRFAVLGHAVLEQALEPWPGITCKALFVDPARDADAQAAAWLGALPAGASPR
                     LLAPLPVFGYPGWLPASAEAGFYDEARYFRPLRETPGSASPSRR"
     gene            complement(3085..4116)
                     /gene="pyrC"
                     /locus_tag="VFB53_09215"
     CDS             complement(3085..4116)
                     /gene="pyrC"
                     /locus_tag="VFB53_09215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZN86947.1"
                     /translation="MDRLTILRPDDWHLHLRDGPQMAAVLPDTARRFARAIVMPNLKA
                     PVRSTQEALAYRGRILAALPEDADFEPLMTLYLTDDTPPEEIARAKRSGQVHGVKLYP
                     AGATTNSDSGVTRISRCFNALEKMEEQGLPLLVHGEVTDPEVDVFDREAVFLEDVLGP
                     LLGRFPNLKVVLEHVTTREAVQFVEVTGANVAATITAHHLLLNRNALFHGGMRPHHYC
                     LPVLKREEHREALVAAATSGNPKFFLGTDSAPHARDTKEAACGCAGVYTAHAALELYA
                     VAFEEAGALDRLEGFASRFGPQFYNLPVNQGRVTLQRGDWQVPASLPFGLGQLVPLRA
                     GETLPWKIA"
     gene            complement(4126..4956)
                     /gene="rsmI"
                     /locus_tag="VFB53_09220"
     CDS             complement(4126..4956)
                     /gene="rsmI"
                     /locus_tag="VFB53_09220"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZN86948.1"
                     /translation="MSPGTLYVVATPIGNLADASARVLDTLRQAGLVACEDTRTTRTL
                     LARHGIGAATLALHAHNERAAGAELLSALQAGKSVALVTDAGTPGVSDPGALLVESAH
                     RAGIRVVPVPGASAAIAAYSASGFAADRFLFAGFLPAKAGARRKALEELDLPWPVILY
                     EAPHRVLETVEDLAARFGGERELVIARELTKKFEEIARLPLGQAAAWLAAGPHRQQGE
                     FVLVLAPGAAAAPDALDAERVLGVLLEALPASEAAKLAARITGAPRNALYKRALELAK
                     "
     gene            4994..5335
                     /locus_tag="VFB53_09225"
     CDS             4994..5335
                     /locus_tag="VFB53_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZN86949.1"
                     /translation="MNSPGVRAEELCADLLRRAGLRILARNWRCRLGEIDLVAEEAGT
                     LVFAEVRLRRNARYGGAAESITAAKRARLLAAARLYLAGCAPAPCRFDVLLLDALEAP
                     RVEWIRGAFGE"
     gene            5399..>5601
                     /locus_tag="VFB53_09230"
     CDS             5399..>5601
                     /locus_tag="VFB53_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZN86950.1"
                     /translation="MGHAEQSAALAARVAAHFADSAQLKLAAAPALVAPIARAAAAMS
                     AALQAGGKVLACGNGGSAADAQH"
BASE COUNT          769 a         1962 c         2063 g          807 t
ORIGIN      
        1 ccgaagaagg cttcgaaggc ctgattgacg tagctcagcg gccgcgaggc gtcggcggca
       61 tcggccagcg ccaccggcag gccgcaggca tcgagcgcgg cgcgcgacag cgcggcctcg
      121 gccagcagcc ggaacagcgc gggcgcgatc tccgggcgcc tgccggcggt ttgccgttcg
      181 gggagtgcca tgcagcgttt gtgccacggg cgcgcgccgc gggcaagcgc ggcggcgcga
      241 aattgcgccc gaacgtgaca aaccccacag tggacgcccg ccgggcgggg cgctaggcgg
      301 ccttggcgtc cttgcgcgac agcgagtagc agaagcggcg caggcgcttc aggtccgcct
      361 gctcgatggc gtccttgagg aagtgcatgt tgcggtcgat ctcggtctgg tacaggctcg
      421 ggccgtcgaa gtgcgcctcg gcgaaacggt ccacgccgcc cagcttcttt tcgatgcgcg
      481 cgcgcgccac gtactcgtag aagccggggg tcttgagcat cagatcctcc ggcgaggcgt
      541 agcgcacgat ctcgcgtccg tcggcgaggg tttcgcgcgc gatgtggccc cagacgaatt
      601 ccgtatacag gaactcgcgg catttctcca ggtacatgcg atccgacatc tggccgatca
      661 ggtcggcggt gcccaccagg cagccgagca ggcggtcctt ggggtcgtgc agcatgatgt
      721 cgtcgatgtc catctcgtag ccggtgaagt gcaccagctt ggaggcggcc ccggcctccg
      781 cggcgaagcc gacctgcggc aggtagcgcg cgagaaaatc cgcgctgcgg ctgacgtgca
      841 ccttggtgaa caccgcgccg ttctcgatgt ggctctcgga ggcgcgcttc aggtagcccg
      901 agtcgtgcag cagggcgacc accacgccga gcatggcgcg ccgcgcgccg acctgctcgg
      961 ccggggcgcg gctgcggtcg tggccgtcga gcatgcgcgc catcgccagc gtcatgtcga
     1021 gcgtatggcg caggtcgtgg tacagcgtgt cgcagggcag gtagccgggg taggtgccgc
     1081 cgaacagcga cttcacgtcg tcgaaggcgc gccgcagcgg cgccaggtcg gcgccgggat
     1141 agcgggcggt gaacagcgcg agcaccgcgt cgcggacctg gtccggatcc tcgacgttga
     1201 tcctattggt aacgtcgttc tggttgaggc gctcgttcac ctagccatcc tgtcgaccct
     1261 gctcccacct tacgccttct tcttattcac gccgcatggg ctgaaacgcc gtcgtcatgg
     1321 cccgatcggc ccaccatccg gccgttcgaa acgatgcacg gcattatccc gctgtcttgt
     1381 cgccgaacgc aaaaaaattt actgcggcgg cgcaaaagat cgcaaggaat tccgggaaaa
     1441 cgggagacgg cctgtaagcc gggttctgtg cgaaggggcg aacccctccc gacagccatt
     1501 cctctgggcc cggagttgcc cccgggctca agccacctac ccgcaagctc ggccgagccg
     1561 gcctcaggcg cttgcctatt tggtgttgct ccggatggag gttgcccgtt tcacccctcc
     1621 cgttgccgga aggactcgtc tctgtggccc tgttcgtcgc cttggcgccg cttgcgcggc
     1681 ggctatgcgc gccggggggt taccccgcat cccgctctgt ggagcccgga ctttcctccg
     1741 cgtcgccgca gcggctgccc ggccatctcc cggggcgcat tctacgcctc gccgggcgcc
     1801 cagcggcttg aaatcccgcc cgtcgaggcg taaacagggg gcgcgggcaa gcgacaaagg
     1861 aggcttccga tgcgcagacg cctgtatttc ctgctccccg acgtggatag cgcgcgccgc
     1921 acggcgaacg acctgctgct ggcgcgcatc gaagaccgcc acatgcattt cctcgccagg
     1981 cgcggcacgc agctcggcga gctgccggag gcgagctacc tgcagaaatc cgacctggtg
     2041 cacggcgcgg gcatcggctt cgcgctcggc ggcctcggcg gcttgctgct tggcgcgctg
     2101 atcgtcgcct ttccgatcga gggcacgcag ccgcagctgg tggcggtgcc ggtcgcggcc
     2161 gtcgtcggcg cgctgctcgg cgcctggatg gcgagcatgg cggccgccgc ggtgcccaat
     2221 tcgcgcctgg aggcgttcca ggacgccatc gccggcggca aggtcctgct gatgctcgac
     2281 gtgcccctgg cgcgggtgga ggaggcgcgc gacctggtgc gcacgcagca tcccgaggcg
     2341 ctcagcggcg ggatggagac gcgctacccg gcgtttccct gagcggcctg aagtagcgcg
     2401 cctcgtcgta gaacccggcc tcggcgctcg ccggcagcca gcccggatag ccgaacaccg
     2461 gcagcggcgc gagcaggcgc ggcgaggcgc cggcgggcag cgcgccgagc catgcggcgg
     2521 cctgcgcatc ggcatcgcgc gccggatcga cgaacagcgc cttgcaggtg atgccgggcc
     2581 agggctcgag cgcctgttcg agcacggcgt ggcccagcac ggcgaagcgc atcgccgcgc
     2641 gcacgcgctc gcgccggtcc cagaacagtt ccttccagcg gcattcccgg accagcgcat
     2701 tcaggccctc gtcggcgcag aggaccagcg cgccgccctc gtcgaaaatg gtgagcaggt
     2761 cgcgcagccg cccgcgcttg ccgccctcgc gcgggatctc ggcggcgtgc agcgcgttca
     2821 cgcgcgcctt ggtgcgcgga aacgccagcc agcacagggc gttgaacagg tcgtggcggt
     2881 tgcccgcgcg cgtctgaacc tggccggtct cgaacagctg catttcgtag gtcgggccgc
     2941 cgcccgcggg cggcacgaag cgcagcggcc tgccgctctc ggtgcgcagc ccccggctct
     3001 cggcggcggc gttcagcgcc gccagcgccg gcgcgggccc gagcgccgcc agccagggcc
     3061 gcagcgggtc gtagaccggg tgggctaggc gatcttccag ggaagcgtct cgccggcgcg
     3121 caacggcacc agctgcccga ggccgaaagg cagcgaggcg ggcacttgcc agtcgccgcg
     3181 ctgcaaggtg acgcggccct ggtttaccgg caggttgtag aactgcggcc cgaagcggct
     3241 ggcgaagccc tccagcctgt cgagcgcgcc ggcctcctcg aaggccaccg cgtagagctc
     3301 cagcgccgca tgcgccgtgt acacgccggc gcagccgcag gcggcctcct tggtgtcgcg
     3361 cgcgtgcggc gcgctgtcgg taccgaggaa gaacttggga ttgcccgagg tcgccgcggc
     3421 caccagggcc tcgcggtgtt cctcgcgctt gagcaccggc aggcagtagt ggtgcggccg
     3481 catgccgccg tggaacagcg cgttgcggtt cagcagcagg tgatgcgcgg tgatggtggc
     3541 cgcgacgttg gcgccggtca cctcgacgaa ctgcaccgct tcgcgcgtgg tgacatgctc
     3601 gagcaccacc ttgaggttcg ggaagcgtcc gagcaggggc cccagcacgt cctcgaggaa
     3661 caccgcctcg cggtcgaaca cgtccacctc gggatcggtg acttcgccgt gcaccagcag
     3721 cggcagcccc tgctcctcca tcttctccag cgcattgaag cagcgcgaga tgcgcgtcac
     3781 gccggaatcc gagttggtgg tggcgccggc cgggtagagc ttcacgccgt gcacctgtcc
     3841 cgagcgcttg gcgcgcgcga tctcctcggg cggcgtgtcg tcggtgaggt acagcgtcat
     3901 cagcggctcg aagtcggcgt cctccggcag cgcggcgagg atgcggccgc ggtaggcgag
     3961 cgcctcctgc gtgctgcgca ccggggcctt caggttcggc atgacgatgg cgcgcgcgaa
     4021 gcgccgcgcg gtgtcgggga gcactgcggc catctgcggg ccgtcgcgca ggtgcaggtg
     4081 ccagtcgtcc gggcggagga tggtgaggcg gtccacgggc ggattctact tggccagttc
     4141 aagcgcccgc ttgtacagcg cgttgcgcgg cgcgccggtg atgcgcgcgg cgagcttcgc
     4201 cgcctcgctc gccggcagcg cttcgagcag cacgccgagc acgcgctcgg cgtcgagcgc
     4261 atccggcgcc gcagccgcgc ccggggcgag gaccagcacg aactcgccct gctgccggtg
     4321 ggggccggcg gcgagccagg cggccgcctg ccccagcggc aggcgcgcga tttcctcgaa
     4381 cttcttggtc agctcgcgcg cgatcaccag ctcgcgctcg ccgccgaagc gcgcggcgag
     4441 atcctccacc gtctccagca cccggtgcgg cgcctcgtac aggatcaccg gccagggcag
     4501 gtccagttct tccagcgcct tgcggcgcgc gcccgccttc gccggcagga aacccgcgaa
     4561 caggaagcgg tcggcggcga agccgctggc cgaataggcg gcgatcgccg cgctggcgcc
     4621 ggggaccgga accacgcgga tgccggcgcg gtgcgccgac tcgaccagca aggcgccggg
     4681 atccgacacc cccggggtgc cggcgtcggt caccagcgcc acgctctttc cggcctgcag
     4741 cgcagagagc agctcggcgc cggccgcgcg ctcgttgtgg gcgtgcagcg cgagcgtggc
     4801 ggcgccgatg ccgtgccggg cgagcagggt gcgcgtggtg cgggtgtcct cgcaggccac
     4861 caggccggcc tggcgcagcg tgtcgagcac gcgcgcgctg gcgtcggcga ggttgccgat
     4921 cggcgtggcg acgacgtaga gggttcccgg ggacaaggtc aacgcggctt ggggtgctgg
     4981 cgttaagagt gccatgaact ccccgggcgt gcgagccgag gaactgtgcg ccgacctgct
     5041 gcgccgggcg gggctgcgca tcctggcgcg caactggcgc tgccgcctgg gcgagatcga
     5101 cctggtggcg gaggaggcgg gcaccctggt gttcgccgaa gtaaggctgc gccgcaacgc
     5161 gcgctacggg ggtgccgccg aaagcatcac cgccgccaag cgcgcgcggc tgctggccgc
     5221 cgcgcgcctg tacctcgccg ggtgcgcccc ggcgccatgc cgcttcgacg tgctgctgct
     5281 cgatgcgctg gaggcgccgc gcgtggaatg gatccgcggc gccttcggcg agtaacctgg
     5341 ctgtcgcgcc ggcccgacgc gggggagcgg ccgcacgagc gtgtaaaatc gccccggcat
     5401 gggccacgcc gaacagagcg ccgcgctcgc cgcccgcgtc gcggcgcatt tcgccgacag
     5461 cgcgcagctg aagctggcgg ccgcgccggc gctggtggcg ccgatcgcgc gcgcggcggc
     5521 ggcgatgagc gcggccttgc aggccggcgg caaggtgctc gcctgcggca acggcggctc
     5581 ggccgccgac gcgcagcatt t
//
LOCUS       DASRIE010000216         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3090395, whole genome shotgun sequence.
ACCESSION   DASRIE010000216 DASRIE010000000
VERSION     DASRIE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090395"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFB53_09235"
     CDS             <1..559
                     /locus_tag="VFB53_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZN86951.1"
                     /translation="NPEAMPRLRINRLYADLAGSARNGSNALASQLQEARWMIKNVQQ
                     RFDTILRVSQAIVDRQRRFFEHGEVAMRPMVLREIAELLSLHESTISRVTTQKFMATP
                     RGIFELKYFFGSHVATDTGGAASSTAIRALIKQLVAAEDARSPLSDAAIAQILGQQGI
                     VVARRTIAKYRESLQIPPVNQRKSL"
     gene            586..912
                     /gene="raiA"
                     /locus_tag="VFB53_09240"
     CDS             586..912
                     /gene="raiA"
                     /locus_tag="VFB53_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZN86952.1"
                     /translation="MNLNVSGHHLDVTPAIRGYVQGKLERITRHFDHVIDAHVILSVD
                     KLRQKAEVTLHLRGKELHCASEDQDLYAAIDLLADKLDRQVLKYKGKRSDKPHAALKR
                     ADADNP"
     gene            1002..1487
                     /gene="ptsN"
                     /locus_tag="VFB53_09245"
     CDS             1002..1487
                     /gene="ptsN"
                     /locus_tag="VFB53_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HZN86953.1"
                     /translation="MGLVAKLLPPANVLLDLQVSSKKRLFEQVGLLFENNHGIARSLV
                     FDSLFARERLGSTGLGQCVAIPHGRIKGLKDALGAFVRAAQPVAFDAPDGKPVQLLFV
                     LLVPEQATEKHLQILSELAQMFSDRALRESMAAAPDAGALHQLIAAWQPDAAGQRHAV
                     A"
     gene            1456..>2063
                     /locus_tag="VFB53_09250"
     CDS             1456..>2063
                     /locus_tag="VFB53_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HZN86954.1"
                     /translation="MLQVNVTQLHEDNRDALALAWVAGRDSDTVVRREAAASAALIGH
                     LNLTHPNSIQVVGAYEAGSLPGLAEHLYASPPAALIIADGVAAPAALLEGAARSKTPL
                     FTTPLSAARVIEKLSRYLAKALAESTERHGVLMDVLGLGVLITGDSGVGKSELGLELI
                     SRGHGLVADDVVEVSRMSGVLEGRCPALLKDFIEVRGLGVLN"
BASE COUNT          333 a          745 c          695 g          290 t
ORIGIN      
        1 caaccccgag gccatgccgc ggctgcgcat caaccggctg tacgccgacc tcgccggcag
       61 cgcgcgcaac ggctcgaacg cgctcgcctc gcagttgcag gaagcgcgct ggatgatcaa
      121 gaacgtgcag cagcgcttcg acaccatcct gcgcgtgtcg caggccatcg tcgaccggca
      181 gcgccgcttc ttcgagcacg gcgaggtggc gatgcggccg atggtgctgc gcgagatcgc
      241 cgagctgctc agcctgcacg agagcaccat ttcgcgcgtc accacgcaga agttcatggc
      301 cacgccgcgc ggcatcttcg aactgaagta cttcttcggc agccacgtcg ccaccgacac
      361 cggcggcgcg gcctcctcca ccgcgatccg cgcgctgatc aagcagctgg tggccgcgga
      421 ggacgcgcgc tcgccgctgt cggacgcggc catcgcgcag atcctcggcc agcagggcat
      481 cgtggtggcg cggcgcacca tcgcgaagta ccgggaatct ttgcagatcc cgccggtcaa
      541 tcaacgcaag agcctctgac ttgccacctc gccgcaggag cgcacatgaa cctgaacgtc
      601 agcggacacc acctcgacgt gactcccgcg atccgcggct acgtgcaggg caagctggag
      661 cgcatcacac ggcacttcga ccacgtcatc gacgcgcacg tcatcctgtc ggtggacaag
      721 ctgcggcaga aggccgaggt cacgctgcac ctgcgcggca aggagctgca ctgcgccagc
      781 gaggaccagg acctgtacgc cgccatcgat ctgctggccg acaagctgga ccgccaggtg
      841 ctcaagtaca agggcaagcg cagcgacaag ccgcacgcgg cgctcaagcg cgcggacgct
      901 gacaatcctt gaccctcgcc cccggcgggc ggcggccgcc gccttgggct ataatccgcg
      961 ccctctgcag tgccgccccg ccggggcctg accggtagac catgggtctc gtcgccaaac
     1021 tgctgccgcc cgccaacgtg ctgctcgacc tgcaggtgtc gagcaagaag cgcctgttcg
     1081 agcaggtcgg cctgctgttc gagaacaatc acggcatcgc ccgcagcctg gtgttcgaca
     1141 gcctgttcgc gcgcgagcgc ctgggctcca ccggcctggg ccagtgcgtc gccatcccgc
     1201 acgggcgcat caagggcctg aaggacgcgc tcggcgcctt cgtgcgcgcg gcgcagccgg
     1261 tggcgttcga cgcccccgac ggcaagccgg tgcagctgct gttcgtgctg ctggtgccgg
     1321 aacaggccac cgagaagcac ctgcagatac tgtcggaact ggcgcagatg ttcagcgacc
     1381 gcgcgctgcg cgagtcgatg gccgcggcgc cggatgccgg cgccctgcac cagctgattg
     1441 ccgcatggca gcccgatgct gcaggtcaac gtcacgcagt tgcatgagga caaccgcgac
     1501 gctctagcgc tggcgtgggt cgccgggcgc gacagcgaca cggtggtgcg ccgcgaggcg
     1561 gccgcctcgg ccgcgctcat cggccacctc aacctcacgc atcccaacag catccaggtg
     1621 gtcggcgcct acgaggccgg cagcctgccg ggcctcgccg agcacctgta cgcctcgccg
     1681 cccgcggcgc tgatcatcgc cgacggcgtc gccgcgccgg cggctctgct cgagggcgcc
     1741 gcgcgcagca agacgccgct gttcaccacg ccgctgtccg cggcgcgcgt catcgagaag
     1801 ctgtcgcgct acctggcgaa ggcgctcgcc gaaagcaccg agcgccacgg cgtgctgatg
     1861 gacgtgctcg gcctgggggt gctgatcacc ggcgattccg gcgtcggcaa gagcgagctc
     1921 gggctggaac tgatcagccg cggccacggc ctggtggccg acgacgtggt cgaggtctcg
     1981 cgcatgagcg gcgtgctcga gggccgctgc ccggcgctgc tcaaggactt catcgaggtg
     2041 cgcgggttgg gcgtgctcaa cat
//
LOCUS       DASRIE010000217         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3615062, whole genome shotgun sequence.
ACCESSION   DASRIE010000217 DASRIE010000000
VERSION     DASRIE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3615062"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFB53_09255"
     CDS             complement(<1..1569)
                     /locus_tag="VFB53_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZN86955.1"
                     /translation="MSGSPQQPQYQSLYRACIKDAATGGADLMRATLERALRELPAMA
                     ARIDDVVDRNLSLDAAGVLREQQQVLVDAFPQALLAEFAQAIAGGRSSAFSFDSLPLL
                     ADEQLQENADLVRGAQELEAAVRPELARLEAMLAANRLTPEGGEQRHPLRPEVYVRSI
                     YRLVRQSPVSPAVRRRWLRHLPPLMAPDLADAYAAIAVRLQAGSALTPDIDLDAPEPQ
                     EAQDRATQLTIRELRKLLAGEREEGVNPPGTAFGESEFSHTVPAALEMAQDLRKVDQL
                     LLQLRQRQAAMPGQGRDSMEAFREALRQEARRPAQTLGLEVVHLMIDHLAGDPRLLPA
                     VRDVVRDLEAPLLRLALADPRFFSDRSHPARQLLEQVTQRSLAWSSADDPGFATFMEG
                     LQEATEALLDAGGGGPETYEIALSALQEAWDEAQPRLRRNRERAVRALMRAEQRNLLA
                     ERLARQLLERPDAPGAPPEALAFLTGPWAQVMAQARLVDATGSEDPGGFAWVVQTVLW
                     SVQPAQAARVAGQHR"
     gene            complement(1591..3246)
                     /gene="ettA"
                     /locus_tag="VFB53_09260"
     CDS             complement(1591..3246)
                     /gene="ettA"
                     /locus_tag="VFB53_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZN86956.1"
                     /translation="MAQYVYTMRRVQKIVPPKREILKNISLNFFPGAKIGVLGLNGSG
                     KSSLLKIMAGEDTNYEGEVLRMPNIKVGFLPQEPVLDPQKTVREEVEQGQGDLAAAKK
                     RLDEVYAAYAEPEADFDKLAAEQASLEAIVNAGGGAQELEIAADALRLPPWEAVIKNL
                     SGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLARFPGTVVGITHDRYF
                     LDNAAEWILELDRGRGIPYKGNYSNWLEQKEARLETEQRTEDARTKAMKKELEWARSN
                     PKGRQAKSKARLARFEELSDYEYQKRNETNEIFIPVGERLGHEVIEFVNVSKSFGDRM
                     LIDNLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGKTVQMAFVDQHR
                     DELANEKTVWEDVSGGLDMINIGKFTMASRAYCGRFNFNGGDQQKKVGLLSGGERGRL
                     HLAKTLLQGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIATHI
                     LAAEDEGKWVFFDGNYQEYEADKRKRLGEEGARPHRVRYKALK"
     gene            complement(3246..>5331)
                     /locus_tag="VFB53_09265"
     CDS             complement(3246..>5331)
                     /locus_tag="VFB53_09265"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN86957.1"
                     /translation="AAPGVLAVYTGADVLADGVGEIPFTRMHKREDGSPIVAPARMPI
                     TAGEARFVGDGVAMVVAETRDQARDAAESIAVDWEPLEAVAGLEAAAQAGLAAAYYHM
                     GDRAKREAAFAAAKHVVRLKLVNQRVVANPLEPRALAAQYDAAAQRYTLWCPTQNVHT
                     TRAQLAVALGVPEAGVRVLSTDVGGGFGTRNCAYPEHAALLWAARKLGRPLRWLADRA
                     ENFLAEVHGRDNLTVAELALDAEHRFTALRIRTLANVGAYISNFGAAVPAMSGARAPT
                     GVYAIPVLDHEVRMLFTNTTPVDAYRGAGRPEMGYLLERLVERAARELGVDAVELRLK
                     NMVQPAQMPYPNPAGYSYDCGDFAKMLRGARDAADWNGYAKRKAAAEKRGLRYGRGMA
                     CYVEITGSARLTEMVRMIAAADGSVQMASGTQAIGQGLWTSYAQVVAQKLGIEPQAVR
                     VLQGDTDLVPVGGGSGGSRGLQVGGSAAFVGAAALIEAARPLAAQALEAAASDLEYGA
                     GRFRIAGTDRGIGLFELAARQPEKRIACEHTETVKGQTWPNGCQVAEVEVDPETGMVR
                     VARLAALDDIGRVINPLTAESQVHGGMAQGLGQALFEQSVYDETGQLITGSFMDYAMP
                     RADQMPPLETRFDESVPTAMNALGAKGAGEAGCHGATPAIVNAVIDALGVKEIDMPLT
                     PEKVWRALREDR"
BASE COUNT          684 a         1948 c         1824 g          875 t
ORIGIN      
        1 gcgatgctgg ccggccacgc gcgcggcctg cgccggctgc acgctccaca gcacggtctg
       61 caccacccac gcgaatccgc ccggatcttc gctgcccgtc gcatccacca ggcgcgcctg
      121 ggccatcacc tgcgcccacg ggccggtgag gaaggccagg gcctcgggcg gtgcaccggg
      181 agcgtccgga cgttccagca gctgccgcgc cagccgctcg gcgagcaggt tgcgctgctc
      241 cgcccgcatc agcgcgcgca ccgcgcgctc gcgattgcgg cgcaggcgcg gctgggcttc
      301 gtcccaggcc tcctgcagcg ccgacagcgc aatctcgtag gtttcggggc cgccgccgcc
      361 cgcgtcgagc agcgcctcgg tggcttcctg cagtccctcc atgaaggtgg cgaagccggg
      421 gtcgtcggcc gaactccacg ccaggctgcg ttgcgtgacc tgctccagca actggcgcgc
      481 gggatggctg cggtcgctga agaagcgcgg gtccgccagg gccaggcgca gcagcggcgc
      541 ttcgaggtcg cgcacgacgt cgcgcaccgc cggcagcagg cgcgggtcgc cggccaggtg
      601 gtcgatcatg aggtgcacga cttcgagccc cagggtttgc gcgggacgcc tggcctcctg
      661 ccgcagcgcc tcgcggaagg cctccatgct gtcgcgcccc tggccgggca tggcggcctg
      721 ccgctggcgc agctgcagca gcagctggtc gaccttgcgc aggtcctgcg ccatttccag
      781 cgccgcgggc acggtgtggg aaaactcgct ttccccgaaa gcagtcccgg gcgggttcac
      841 gccctcctcg cgttcccccg ccagcagctt gcgcagttcg cggatggtga gctgggtggc
      901 gcggtcctgc gcctcctgcg gctcgggcgc gtccaggtcg atgtcggggg tgagcgcgga
      961 gccggcctgc aggcgcacgg cgatcgcggc ataggcatcg gccaggtccg gcgccatcag
     1021 cggcggcagg tggcgcagcc agcggcggcg taccgccggc gataccgggc tttgccggac
     1081 caggcgatag atgctgcgca cgtagacttc cgggcgcagc gggtggcgct gctctccgcc
     1141 ctcgggggtg aggcggttcg ccgccagcat cgcttccagc cgcgccagct ccggccgcac
     1201 ggcagcctcg agttcctgcg cgcctcgcac caggtcggcg ttttcctgca gctgctcgtc
     1261 ggccagcagc ggcagcgaat cgaagctgaa ggcggagctg cggccgccgg cgatcgcctg
     1321 cgcgaactcg gcaagcagcg cctgcgggaa agcgtcgacc agcacctgct gctgctccct
     1381 cagcacgccg gccgcatcga gcgacaggtt gcggtccacc acgtcgtcga tgcgggccgc
     1441 catggccggg agttcgcgca gcgcccgctc cagggtcgcc cgcatcaaat cggcgccccc
     1501 ggtggcggcg tccttgatgc aggcgcgata gagcgactgg tactgcggct gctgcgggga
     1561 gccgctcatg gcggcctgcc tcctcgggtt ctacttcagg gctttatagc gcacccggtg
     1621 cggtcgcgca ccttcttcgc cgaggcgctt gcgcttgtcg gcttcgtatt cctggtagtt
     1681 gccgtcgaag aacacccact tgccctcgtc ttccgcggcc aggatgtggg tggcgatgcg
     1741 gtccaggaac cagcggtcgt ggctgatcac catcacgctg ccggcaaact ccagcagcgc
     1801 gtcttccagg gcgcgcaggg tttccacgtc gaggtcgttc gagggttcgt ccagcagcag
     1861 cacgttgccg ccctgcagca gggtcttggc caggtgcagc cggccacgct cgccgccgga
     1921 cagcaggccg accttcttct gctggtcgcc gccgttgaag ttgaagcggc cgcagtaggc
     1981 gcggctggcc atcgtgaact tgccgatgtt gatcatgtcg aggccgccgg agacgtcctc
     2041 ccacacggtc ttctcgttgg ccagttcgtc gcggtgctgg tcgacgaagg ccatctgcac
     2101 ggtcttgccg atcttcacct cgccgctgtc cggctgctcc ttgccggcga tcatcttgaa
     2161 cagcgtggac ttgccggcgc cgttcggtcc gatgatgccg acgatggcgc cggccggcac
     2221 cttgaagctc aggttgtcga tcagcatgcg gtcgccgaac gacttggaga cgttcacgaa
     2281 ctcgatcact tcgtgcccca ggcgctcgcc caccgggatg aagatctcgt tggtctcgtt
     2341 gcgcttctgg tattcgtagt cggacagttc ctcgaagcgg gccaggcggg ccttgctctt
     2401 ggcctggcgg cccttcgggt tggagcgcgc ccactccagt tccttcttca tcgccttggt
     2461 gcgggcgtcc tcggtgcgct gctcggtttc caggcgcgct tccttctgct ccagccagtt
     2521 ggagtagttg cccttgtagg ggatgccgcg gccgcggtcc agttccagga tccactcggc
     2581 cgcgttgtcc aggaagtagc ggtcgtgggt gatgcccacc acggtgcccg ggaagcgcgc
     2641 gaggaactgc tccagccatt ccaccgactc ggcgtccagg tggttggtgg gctcgtccag
     2701 cagcagcatg tccggcttgg acagcagcag gcggcacagg gccacgcggc gcttctcgcc
     2761 gcccgacagg ttcttgatca ccgcctccca gggcggcagg cgcagcgcgt cggccgcgat
     2821 ctccagctcc tgggcgccgc cgccggcgtt gacgatcgcc tccaggctcg cctgctcggc
     2881 ggcgagcttg tcgaaatccg cctcgggctc cgcgtaggcg gcgtagacct cgtccaggcg
     2941 ctttttcgcc gccgccaggt cgccctggcc ctgctccacc tcctcacgca ccgtcttctg
     3001 cgggtcgagc acgggctcct gcggcaggaa accgaccttg atgttcggca tgcgcagcac
     3061 ttcgccctcg tagttcgtgt cttcccccgc catgatcttc agcaggctcg acttgccaga
     3121 gccgttcagg cccagcacgc cgatctttgc gccggggaaa aaattgagcg agatgttctt
     3181 caggatctcg cgcttcggcg gcacgatctt ctgcacccgc cgcatggtgt acacgtattg
     3241 ggccatcagc ggtcctcgcg cagcgcgcgc cagactttct cgggagtgag cggcatgtcg
     3301 atttccttca cgccgagcgc gtcgatcacc gcgttgacga tggcgggcgt ggcgccgtgg
     3361 cagccggcct cgccggcgcc cttcgcgccg agcgcgttca tcgcggtggg caccgattcg
     3421 tcgaagcggg tctccagcgg cggcatctgg tcggcgcgcg gcatggcgta gtccatgaag
     3481 gagcccgtga tgagctgacc cgtctcgtcg tataccgact gctcgaagag ggcctgcccc
     3541 agcccctgcg ccatgccgcc gtgaacctgc gattccgcgg tgagcgggtt gatcacgcgg
     3601 ccgatgtcgt ccagcgccgc gagccgcgcg acgcgcacca tgccggtttc gggatccact
     3661 tccacctcgg ccacctggca gccgttcggc caggtctgcc ccttcaccgt ttccgtgtgc
     3721 tcgcaggcga tgcgcttctc cggctgccgc gccgccagct cgaacaggcc gatgccgcgg
     3781 tcggtgccgg cgatgcgaaa gcgccccgcg ccgtactcga ggtcggacgc cgccgcctcc
     3841 agcgcctgcg ccgcgagagg gcgcgccgcc tcgatgagcg cagccgcgcc cacgaaagcc
     3901 gcgctgccgc cgacctgcag tccgcgcgag ccgcccgagc cgccccccac gggcacgaga
     3961 tcggtgtcgc cctgcagcac ccgcaccgcc tgcggctcga tgccgagctt ctgcgccacc
     4021 acctgcgcgt aggaggtcca caatccctgg ccgattgcct gggtgcccga ggccatctgc
     4081 acgctgccgt ccgcggctgc gatcatgcgc accatctcgg tcagccgcgc gctgccggtg
     4141 atctcgacgt agcaggccat cccgcgccca tagcgcaggc cgcgcttctc ggcggccgcc
     4201 ttgcgcttcg cgtagccatt ccagtccgcc gcgtcccggg cgccgcgcag catcttcgcg
     4261 aagtcgccgc agtcgtagct gtagcccgcc gggttcgggt agggcatctg cgccggctgc
     4321 accatgttct tcaggcgcag ctcgaccgcg tccacgccca gctcccgcgc ggcgcgctcg
     4381 accaggcgct cgagcaggta gcccatctcc ggccggccgg cgccgcggta cgcatccacc
     4441 ggcgtcgtgt tggtgaacag catcctcacc tcgtgatcga gcaccgggat ggcgtacacg
     4501 ccggtcggcg cgcgcgcgcc ggacatcgcc ggcaccgcgg cgccgaaatt ggagatatag
     4561 gcgccgacgt tggcgagcgt gcggatgcgc agcgccgtga atcggtgctc cgcgtccagc
     4621 gcgagctccg ccaccgtgag gttgtcgcgg ccgtgcacct cggcgaggaa attctccgcg
     4681 cggtcggcca gccagcgcag cgggcggccc agcttccgcg ccgcccagag cagcgccgcg
     4741 tgctcggggt aggcgcaatt gcgcgtgccg aagcccccgc ccacgtcggt gctgaggacc
     4801 cgcacgccgg cctcgggcac gccgagcgcg acggcgagct gcgcgcgcgt ggtgtgcacg
     4861 ttctgcgtcg ggcaccagag cgtgtagcgc tgcgcggccg catcgtactg cgcggcaagc
     4921 gcgcgcggct cgagcgggtt cgccaccacg cgctggttga ccagcttgag gcgcacgacg
     4981 tgcttcgccg ccgcgaaggc cgcctcgcgt ttcgcgcgat cccccatgtg gtagtaggcg
     5041 gccgcgagac ccgcctgcgc cgccgcctcg aggcccgcca ccgcctccag gggctcccag
     5101 tcgacggcga tgctctctgc cgcgtcgcgc gcctgatcgc gggtttcggc caccaccatc
     5161 gccacgccgt cgccgacgaa gcgcgcctcg cccgccgtaa tcggcatgcg tgccggggcg
     5221 acgatgggcg aaccgtcctc ccgcttgtgc atgcgggtga agggaatttc gccgacgccg
     5281 tcggcgagga cgtccgcccc ggtataaacc gcgagcacgc cgggcgcggc g
//
LOCUS       DASRIE010000218        29999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_234362, whole genome shotgun sequence.
ACCESSION   DASRIE010000218 DASRIE010000000
VERSION     DASRIE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 29999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234362"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VFB53_09270"
     CDS             <1..1269
                     /locus_tag="VFB53_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN86958.1"
                     /translation="RLISTLKRLRDLGNSVIVVEHDAEAILAADHVVDMGPGAGVAGG
                     RVVAEGPPEAILESESSLTGKYLSHRLRIAVPRLRNRPSAKRALRIRGASGNNLKQVE
                     FELPVGLFVCVTGVSGSGKSTLVNDTLYRAAARHLNGSTDEPAPYESISGLEQFDKVV
                     SVDQSPIGRTPRSNPATYTGLFAPIRELYAGVPEARTRGYDAGRFSFNVKGGRCEACQ
                     GDGLIKVEMHFLPDIYVPCDVCHGKRYNRETLEIRYKGRSIHEVLQLNVREAHEFFAA
                     VPAIARKLQTLLDVGLGYVQLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEPT
                     TGLHFADIQLLLDVLHRLRDAGNTVVVIEHNLDVIKTADWAIDLGPEGGDGGGRIVAQ
                     GTPETVARNPGSHTGRALKQVLEVPKKQAA"
     gene            1301..1924
                     /locus_tag="VFB53_09275"
     CDS             1301..1924
                     /locus_tag="VFB53_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN86959.1"
                     /translation="MNKPTKGEQTRAAILDQAVDIASRVGLEGLTIGTLAEATGMSKS
                     GLFAHFGSREGLQLATLEHVKARFGEQVLTPVLGVERGLPRLRALFLAWIGWKAAPGP
                     GGCVLQSAAHEYDDRPGLIRDSVVDGQRRGLAMIERAVRVAIEEGHLRAETDPAQIAF
                     EMYGQILVAHNHSRLLGDREGRKRALFAFDELVARHAATPPRQRAAA"
     gene            1966..2874
                     /locus_tag="VFB53_09280"
     CDS             1966..2874
                     /locus_tag="VFB53_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86960.1"
                     /translation="MNSTNVRFLFETLLRRAGLGASALLAPGLAGLWAERLFLTPPKP
                     RYPSSEFFDLIDARQSFLRHRGRHIASWRWGSPDAPAVLLVHGWGGRATQMRRFVAHL
                     TAAGFRVIAYDQPAHGLSEGRLTGLPDFADVLLAVARHHGKVHAVISHSLGGPAAAIA
                     LARGLAASRAVLISPPSDLVGYSRRFARWLALPERVRRSMQAAIEERFGVRWSELEIA
                     RVAPLLRAEALVIHDRQDTQVPWRQGAALARHWPGARLMSTVGLGHGRILEDDAVARA
                     AVDFIAGRSAVAQAALPSLPNPAPLL"
     gene            2876..3358
                     /locus_tag="VFB53_09285"
     CDS             2876..3358
                     /locus_tag="VFB53_09285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN86961.1"
                     /translation="MDRSLTTTWSDPLAAFEEGRKLAPIEHLRAIRDGRLPDPPIARL
                     LGMRLVKVEPGEAVFELTPAEQHYNPIGVVHGGVAFTLLDSAMGCCVQTTLPAGKGYT
                     TLEMKANLVRAVTLQTGLIRATARVVHAGKTTATAEARLEDAAGKLYAHGTSTCLILA
                     "
     gene            3415..4080
                     /gene="ypfH"
                     /locus_tag="VFB53_09290"
     CDS             3415..4080
                     /gene="ypfH"
                     /locus_tag="VFB53_09290"
                     /inference="COORDINATES: protein motif:HMM:NF008525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN86962.1"
                     /translation="MPGKNFRFGPTPEHRAQHLFVFLHGCGATSKSMIPIAFRFQARF
                     PSAALVVPSGFASAEVGGEAQQWYSSRGLTDENRLERVAAILPRLEDLVRREQTNCGV
                     PMERTVLIGFSQGGTVALEAVKQLPGLAGAVVAYAARFARLPQDGARLGSRIHLVHGE
                     YDAVVSRVHSERAARVLAALHVPVTLDIVDQLGHALSHHAICLGSLRLLQGIYEGRRA
                     TLH"
     gene            4077..4430
                     /locus_tag="VFB53_09295"
     CDS             4077..4430
                     /locus_tag="VFB53_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZN86963.1"
                     /translation="MKQALILFAHGSRDAQWAQPFEALRAALAPHCDVELAYLELMAP
                     TLEEAVARLAARGARRVRVVPVFLGQGAHLKRDLPALAAAAGARHPGLQIALEPAIGE
                     RPEVIAALAAAIGRG"
     gene            4454..5410
                     /gene="cofD"
                     /locus_tag="VFB53_09300"
     CDS             4454..5410
                     /gene="cofD"
                     /locus_tag="VFB53_09300"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZN86964.1"
                     /translation="MALGGQVGGAKLAEGLYRLRGKRLAVIVNTGDDFEYLGLQVSPD
                     MDTMLYALAGIADAAKGWEPAGETFACHGMLARLGAPAEVPLGDRALAFQLLRAEALR
                     AERPATAIALELCQRLGIEARVLPMSNDPVRTIVNTELGAMSFQDYFMELKCEPAVRS
                     FQYGGADEARLSDEVLDALHAGDLEAVVLCPCNPYHSMRPILAVHGMREALRRRGAPV
                     IAVSPIIGGRALQGSAAKMMQELGREVSPRAVAMEYHKLIDGIVIDSADEALAEGLRA
                     SGVEVLVAQTYMRTADDRLQLARAVLEFARAVRLRKEVAADA"
     gene            5450..6376
                     /locus_tag="VFB53_09305"
     CDS             5450..6376
                     /locus_tag="VFB53_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86965.1"
                     /translation="MRKDSLQCLGPHGFHRIAYTEWGDPGAGRVVVCAPGLTRNSRDF
                     DFLAQRLAERCHVICMDVAGRGASDWLPDKSDYGFDLYEADGAALIARATARPRRPLF
                     AWLQRVLPERAAEVAWVGTSMGGIIGMLLAAQPGSPIRRLVLNDVGATVPWSALMRLK
                     GLVRRHEPYANLAAVARHVREACAGFGPLTDSQWRHLARHSVTRLREGGYRLAYDPAI
                     EASLWNGSDLDMPIGPELMRGINLWRHWDAIRAPTLILRGSESDVLPADVARDMLGRG
                     PKARLVEFAGVGHAPALMAADQIDAVEAFLLD"
     gene            complement(6377..7048)
                     /locus_tag="VFB53_09310"
     CDS             complement(6377..7048)
                     /locus_tag="VFB53_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZN86966.1"
                     /translation="MIIVHHLNNSRSQRVLWLLEELGVAYEVKRYQRDAKTMLAPPEL
                     RAVHPLGKSPVIEDGAATLAESGAIIEYLVERYGGGRLAPAAGTPERLRYIYWMHFAE
                     GTVMPPLLLKLVFDRIEKGPMPFFVRPVARAIARRAKSGFVQPNIERNLDFMEGELAK
                     SAWFAGAEFSAADVQISFPLEAAAARGGLDAKRPRLMSFLERIHARPAYRRALDRGGE
                     YALLR"
     gene            complement(7059..9020)
                     /locus_tag="VFB53_09315"
     CDS             complement(7059..9020)
                     /locus_tag="VFB53_09315"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZN86967.1"
                     /translation="MEPMWTPSEEAIEQAQLTQFARHAVRKHRLDLNTYPEFYQWTVD
                     NPELFWNEVWEWCGVRASRKGGTVLVDGDKMPGAHWYPEARLNFAENLLRRGDRGDAL
                     VFWDERGAQRRVSYSDLTSDVSRAVQALQQLGLRVGDRAAAFIPNLPETAMFALAALS
                     QGIVWSSCSPDFGVDGVIDRFGQIEPKILFVADGYLYNGKRHDVLERVREIAERLPSL
                     RKVVVVPYLDARPDISDIPKAVLLEEWLRRHTPGDIPWAQLPFDHPAYILYTSGTTGK
                     PKCIVHGAGGSLLAGLKMYKLQFDVRPGDHFFYYTTCNWVMWNLLFAGLCAEATLMLY
                     DGSPYARDGRILFDYAQRERFSHFGTSARFIDAIAKRGLKPRESHPLPGLRMIVSTGS
                     PLVPESYDYVYRDVKADVCLSSISGGTDIMAAFADANAVLPVYRGELQCRSLGLAVEV
                     FDPEGGSIVGEKGELVCTRTFPSMPLGFWNDRGDEKYHAAYFAKYPNVWCHGDWCELT
                     ARGTMIVHGRSDATLNPGGVRIGTAEIYGQVEKVPEVEESVAIGQLWPPDKPTDTRVV
                     LFVKLREGLRLDAGLQERICRQIGEHTTRRHVPSKIVQVPDIPRTKNNKVVELAVKAV
                     VHGMPVGNTDALANPEALELFRDIPELQS"
     gene            9124..9489
                     /locus_tag="VFB53_09320"
     CDS             9124..9489
                     /locus_tag="VFB53_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZN86968.1"
                     /translation="MPKPAYDCAKCPAYCCSYPEIEVSKADIARLARHFGVDQALAEE
                     RYTKLERQEEVRILRHREDEHFTSVCMFLDRKSRSCTVYASRPYTCRGYPYAARCGYY
                     EFLKFEREHQADESFVATT"
     gene            complement(9490..11172)
                     /locus_tag="VFB53_09325"
     CDS             complement(9490..11172)
                     /locus_tag="VFB53_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN86969.1"
                     /translation="MKSDLKVRHGGRILADALLGHGVQMAFGVPGESYLPVLDGFHDV
                     ADKLKFIVCRQEGGASYMAEAYGKLTGQPGVLFVTRGPGAANGAVGVHTAFQDSTPLV
                     VFVGQVGNDFVEREAFQEIDYRRMYGPMTKWVAQIDRTERIPEYVSHAFHTAMAGRPG
                     PVLLALPEDMLFAEAAVADAPRYKTVRAAPAAADLRELRRLLQGAERPFVLIGGGGWD
                     RASCDGLRAWLEASRLPAGTSFRCQDLLDNRSASFAGDVGIGINPKLAQRVREADVVL
                     VIGARLGEMTTGGYSLFEVPVPRQTLIHVHGGAEELGRVYHAALPINSGMAAFVEALA
                     SVALDGARWAARTAQARDEYLAWTEPQPIPGKLQYAQIVKWLSDRLPEDAIMCGGAGN
                     FSGWVHRFFRYKGFRTQLGSTNGSMGCGLPAAVAAKLAAPQRTVVAVTGDGDFLMNGQ
                     ELATAAQYGAAIVVLVVNNGMYGTIRMHQEREYPGRISGTELKNPDFAAYARAFGGHG
                     ELVEATAQFAPAFERALASGKPALIELRIDPDAITTAATLSAIRKQALERRK"
     gene            complement(11169..11783)
                     /locus_tag="VFB53_09330"
     CDS             complement(11169..11783)
                     /locus_tag="VFB53_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86970.1"
                     /translation="MPSQFNLIWQLLHQFFVVVVLVFSAVGFVVGIGLIVSSARTLRF
                     FHAMNRWISMRGALKPMEIPRDTELLSHGHRRLLGYALIGGGAFSAFGLIAGVDAAAV
                     GASFAKGDMVRIVAIAAAALKWFLVVGSVAGVAVGFLLRFSPDALTSIEKHANRWISS
                     RRAMRGADDLNLTLDRLVEAHPVPWGWILVCTTLGAGAYAAALL"
     gene            complement(11762..12703)
                     /locus_tag="VFB53_09335"
     CDS             complement(11762..12703)
                     /locus_tag="VFB53_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN86971.1"
                     /translation="MSLHPQVAQLLERAAKSPLPPYYEVPAHVARRIYRDTRAALAPR
                     APEVAEARLVIAPGPVALRAYRPAGSKPEEKLPALVYFHGGGWVIGDLDTHDVLCRQL
                     ANGARCAVYSVDYRMGPEHPFPAAVDDCLAATKFVASRHASVAVGGDSAGGNLATVVA
                     LLARDAQGPAIAYQLLIYPATDQRGQHPSVARNGEGYLLTKKSMDYFQAQYLPRREDL
                     ADWRASPLLAKSLAGLPPAYLITAGYDPLLDEGREYAERMAREGVDVAYREYPDMVHG
                     FILMGGVLDTANAAVAECCEKLRSQWTMEDACRPNST"
     gene            complement(12700..13041)
                     /locus_tag="VFB53_09340"
     CDS             complement(12700..13041)
                     /locus_tag="VFB53_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZN86972.1"
                     /translation="MYVEERMYLLQVGKAPEYFKHYEEVGMKVQLRHLPNLVGYYVTE
                     AGMQNLVTHMWAYDDLLQREKMRASMQADPEWQAYLPRIRPLIVTQETKIHKCAPFFV
                     ERLKKMLAAVR"
     gene            13083..13553
                     /locus_tag="VFB53_09345"
     CDS             13083..13553
                     /locus_tag="VFB53_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZN86973.1"
                     /translation="MDDINTLRRILSQNRVIAVVGLSADWFRPSYFAAKYMQEHGYTV
                     IPVNPRYAEVLGQKCYKSLREIPGKVDLVDCFRKTEDILPICDDAIAIGAKVLWQQLG
                     VRNETAAAKARAAGMDAVMDRCVKIEHGRLFGGLNWVGVNTRVISAKRPRWLAY"
     gene            13653..14531
                     /locus_tag="VFB53_09350"
     CDS             13653..14531
                     /locus_tag="VFB53_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN86974.1"
                     /translation="MPRPIRIFEGRFGRLVLAEAAAPGIPAAQGEPLILLKVDGADSV
                     VSIGGTPQPLTREDALFVNPGEAAQTMGSANGAPARVIAYYPSTAWLQASFPAAFEDA
                     GAPMFPRAREAIAPRMRQLADTLAVETLNDQFLSPERLEFMLQEMMLSVIEAYMARRR
                     AGASLWRGSRFADTRIRKALALLRARPNKDVNIDDLASQVGLSRSRFYDLFQVCTGRS
                     PRSYLDLLCVESAIAKLSSGRTRIADVSAELGFSAQSNFTRFFLNQVGIPPSEYRRAA
                     SRPETSDTPPETASRG"
     gene            14598..15344
                     /locus_tag="VFB53_09355"
     CDS             14598..15344
                     /locus_tag="VFB53_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF family methyltransferase"
                     /protein_id="HZN86975.1"
                     /translation="MQDAKALYLDLLKRCLANTIYQDSGTTLGKHLPFDLGHRIEGRD
                     WPVVAHTMIGLKRLDALHFCVQDVLARGVPGDLIETGVWRGGATIFMRGVLKAHGVTD
                     RVVWVADSFEGLPAPDAAKYPADANYNFHAFRELAVSLEQVKQNFQRYGLLDDQVRFL
                     KGWFRDTLPGAPIRSLAVLRLDGDLYESTIDALTHLYPKLERGGYAIIDDYSDVPACK
                     QAVTEYRAAHGITEEIVPIDWTGVFWRKER"
     gene            15378..16184
                     /locus_tag="VFB53_09360"
     CDS             15378..16184
                     /locus_tag="VFB53_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZN86976.1"
                     /translation="MQQRRLGSGALFLTIALAGCAGLSGDASRQELLPTGKLRVGIGV
                     GASPSAFWAVKDPATGQPRGVTVDLANALARKLNAPLQLVVYANSGEVVAAGPKGEWD
                     VAFMPEDAERRKMVDFGPAYYLSTSTFLARAGSGINTLADVDRPGVRAAGIANTTTAR
                     TAISTLKQTKLISFRTVDEVIAKMKAGEIDAIALGRESLQGLAPQIPGSRILDGHFHA
                     AGTAIAVPKGKPAALAYATAFIEEAKRDGTVRGALDGVGLRNAPVAPAAK"
     gene            16207..17496
                     /locus_tag="VFB53_09365"
     CDS             16207..17496
                     /locus_tag="VFB53_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZN86977.1"
                     /translation="MADRKFGFETLSLHAGQIPDPATGARAAPIYQTTSFVFDSADHA
                     ASLFNLQTFGNVYSRISNPTVAVLEERVAALEGGRAALACATGQAAEATAILTLCKAG
                     DHVVSASTLYGGTHTLLGVNLKKLGIDTTFVHPDEPENFRKALKPNTKLLYAETLGNP
                     LINIVDIAAVAAIAKDAGVPFLVDNTVPSPYLCRPIEHGADLVVHSATKYLGGHGTTM
                     GGVIVESGRFPWGNGKFPEFTTPSPGYHGVIFHETFGDFGFTMKARMEIMRTFGPTLA
                     PLNAWLLLQGIETLGVRMDRHCANALAVAKFLQGHKQVAWVNYPGLPDNKYYRLAQKY
                     LPKGASGLLNFGVKGGAKAGEKFIEAAQFMSHLANIGDAKTLIIHPASTTHRQMSDEE
                     QLKAGVTPDMVRMSVGIETLDDILWDIDQALKEAGKA"
     gene            17493..18557
                     /locus_tag="VFB53_09370"
     CDS             17493..18557
                     /locus_tag="VFB53_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HZN86978.1"
                     /translation="MISYQIIEHGKPLQKVMHETPVPKGTEVLVRITRSGVCHSDVHI
                     WDGYFDWGGGKRFYVKDRGCIPPFTLGHEPFGVVAAVGPRAKGVKVGKKFIVYPWIGC
                     GKCPVCKAGQDNYCMKAQFLGVMKPGGYSSHVLIPHPKYLVDAAGIDEGFAATLGCSA
                     ITAYSGLNKLPRLGPKDWIAVIGCGGLGLVALSILKAKGVKNVIACDVDEAKLAAARK
                     QGAKLTLDTRAPDAAQKLGALAMGNLAASIDFVGMPATAMLGTAALRKGGRYVICGLY
                     GGELVHPLPPIAQRAIGIVGSYVGNLQELKEVVALAKKKKLKATPVELRRPEDVNRTL
                     DELKAGKVLGRVVLDFEAAG"
     gene            complement(18554..20401)
                     /locus_tag="VFB53_09375"
     CDS             complement(18554..20401)
                     /locus_tag="VFB53_09375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN86979.1"
                     /translation="MNFFERQEAARRTSRRLLWLLVPAMLGIVLAANCAALFAWWVVK
                     LFLHDLSAWPGALFFLAVSLATLWVIGLGTWDSIRAVAEGGAALAEALGARPLEPRTG
                     DLAEQRLRNVVEEMAIAAGMAVPRVYVMDGEQSVNALAAGFSTADAVLIVTRGLLDEL
                     RRDELQGVIAHEFSHILNGDIRINMRLIGLLGGILKLWHAGQALLDREDQSVQRQRDR
                     GLKPKGNLAYVLGGALLQAVGSLGSFMARLIRAGVSRQRELLADSCAVQFTRDTDGIA
                     GALVKLERHAERGRIHHWRAEQASHMFFGDALEGGRAGRFATHPTVAQRLANIYGRRV
                     TLDEVVKRVSSRPAAAAAEPAAPPAGVADSIGTISRPQVAYASAFLESLPAPLREALH
                     APEGAAVAMCALVLAPAGEARDAQVAMLGARADAVVAVHQSIAALGRTARLPLVELAI
                     PALRALAGDERRNFLARLHAMIAADRRMTLEEFVLEVILASALQEPGRAGGEPELGSL
                     DALVDDTRLMLSLVAHAGGANPAQAFAAGCKELGSAMQPIPVKALTLEGIQGALARLR
                     RLRPLQKPRLLKALACASLADAKLAPAEAELLRAVSAALDCPMPPVAAA"
     gene            20483..22075
                     /locus_tag="VFB53_09380"
     CDS             20483..22075
                     /locus_tag="VFB53_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZN86980.1"
                     /translation="MTPFSWNNPYAWPRKPVLAKNIVSTSQPLAAQAGLQMLAAGGNA
                     VDAILASAITLTLVEPVSNGIGSDAYAIVWDGKEMHGLNASGRSPVGWTPEYFKNEKQ
                     MTQRGWNSVTVPGCISAWRMLSEKFGKLSFEKLFEAAIRYGREGFLVSPTIAGQWAKQ
                     VPELKAQPGFAEAFMPNGRAPQPGEVFKFPDHAATLEAIAKTKGEAFYNGPLAERLES
                     HARQNGGVMKASDLAAHKSDWIAPIKVDYRGYTLHEIPPNGQGIVALMALGMLQHFEL
                     KSHPVDGPDSLHLQIEAQKLAFADAWAYVADLDHMKEVKPAQLLDAGYLKERAKLIDP
                     KKAQDFGPGKPPRGGTVYLAAADASGMMVSYIQSNYMGFGSGVVVPGTGISLQNRGGT
                     FSLKPGHPNQVGPGKRPYQTIIPGFLTKDGKPVMSFGVMGGTMQPQGHAQVVVRIADY
                     GQHPQAACDGPRFRFVQGMDVSVEEGGFKSSTLEELQKRGHRIVNVDDYNQFGSAQLI
                     WKTEGGYIAASDPRRDGQAAGF"
     gene            22078..22695
                     /locus_tag="VFB53_09385"
     CDS             22078..22695
                     /locus_tag="VFB53_09385"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZN86981.1"
                     /translation="MLKIWGRVNSVNVKKALWCVGELGLPHERVDAGMQFGVNNTPEY
                     RKMNPTGLVPTIDDDGFTLWESHSIVRYLCAKHSMGKLCPSELRARADAERWMDWAFT
                     FQNAMRNVFWGLIRTPPEKRDAKAIEDGAKKSHELAALLDQALAGKPYVAGAAFTMGD
                     IPIGAEVQRYMRVPIERPALPHLHAWFERLRGRPAFLKHVDLPLT"
     gene            22692..23447
                     /locus_tag="VFB53_09390"
     CDS             22692..23447
                     /locus_tag="VFB53_09390"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZN86982.1"
                     /translation="MSKVAIVTGAASGIGLACAERLAADGMTVVLADLNEKTGAEHAK
                     RLGGSFVAADLGRREGCRKLIDATLKAHGTVHVLVNNAGFQHVSPIEAFPEDRWEQMI
                     ALMLTAPFLLTRYCWPAMKRQKWGRVVNIASIHALIASPFKVGYIAAKHGLVGLTRTA
                     ALEGGEHGITVNAICPAYVRTPLVDSQIADQAKANRMSPEEVIEKIMLAPAAVKRLIE
                     PAEVADFVAYLCGDAAGTITGAALTMDLGWTAR"
     gene            23510..23950
                     /locus_tag="VFB53_09395"
     CDS             23510..23950
                     /locus_tag="VFB53_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN86983.1"
                     /translation="MAPATVETMAGEEAGLRMSIDGMPVLRCGEGHQRFVTPDFAIRL
                     MQAITGDGPIAPIDAAVQKGLFRKRLHCPACGKELGEPAAERAQISRPLELGGGAAFS
                     VRIELPKYRCAACAKECLPPEAILGDALMKASVHAFRSAALRPT"
     gene            complement(23943..24896)
                     /locus_tag="VFB53_09400"
     CDS             complement(23943..24896)
                     /locus_tag="VFB53_09400"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZN86984.1"
                     /translation="MRAFLLLSAVLLGGCETLSYYGQAAGGQLALMARARPLEEVVAA
                     PALQERLGLARAIRDFASRELALPDNGAYRSYADLGRPYALWNVVAAPEFSLAPVQSC
                     FPIAGCVSYRGYYGKEAAERHASAQRALGHDVVVYGVPAYSTLGWFDDPLLSTFIHYP
                     EADLARLMFHELAHQVAYVKDDSTFNESFAVVVEREGLRRWLAAQGKQPAPRFDPAPQ
                     VAAVRAQLEALYRTRLSPEAMRERKRAALETLRPLVARMPAFEGQEPNNAFVASYATY
                     TDLVPAFDRLLAASGGDLARYYDRVRSLARLPPAERNAQLR"
     gene            complement(24874..25491)
                     /locus_tag="VFB53_09405"
     CDS             complement(24874..25491)
                     /locus_tag="VFB53_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN86985.1"
                     /translation="MRDAVLVHGLWVPALVMTPLAARLQSAGLRSHLFGYAGRERPLE
                     AHGERLARFARGIGPAHFVGHSLGGLVVLETLLRHPATAAGNVVLLGTPVLGNLAGRR
                     LARLGWGRWFLGASEPLWREGRAARWTRPEPLGVVAGTLPVGLGRLFGALPGPNDGVV
                     RLEETAIEGMRQRMVLPVGHSAMLLSARVAAQVGSFLAHARFSAA"
     gene            complement(25497..25913)
                     /locus_tag="VFB53_09410"
     CDS             complement(25497..25913)
                     /locus_tag="VFB53_09410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN86986.1"
                     /translation="MTQIPQGFRPLPSASPFNELVGPLYEKRGEGVVSLGLRVDRKHS
                     NSRDICHGGVLATLADLALGYGMMAKTGGRGGFVTAHLDIDYAGAASVGDWLHSEVEV
                     QRMGSRLAFATGYLCVGETRIVRMSGIFALPGRSAA"
     gene            complement(25924..26823)
                     /locus_tag="VFB53_09415"
     CDS             complement(25924..26823)
                     /locus_tag="VFB53_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZN86987.1"
                     /translation="MPLNRSFLFAPGNHPRRVEKALSLGADAVILDLEDAVAVSDKAA
                     ARRPVAEALKRPRKCRGYVRPNAPSSPYCYRDLVETIHGKVDGVVLPKVESAADLHAI
                     DWLLAALEREQGIAEGSIDLIPQIETAAGVARVDRIVQARNLRPYPGPWRVKRVAFGA
                     ADFSLDTGIVPSHEEPELAEARARVVLASRAAGMEGPLDSPWFHLRDMEGYQRALERS
                     RRGGFQGRLCVHPDQVPPANAAFTPSDEEAARAERIVAAFQAAEAKGAAAVEVDGQMV
                     DYPIVHRARALLETLREIRGRDR"
     gene            complement(26810..27982)
                     /locus_tag="VFB53_09420"
     CDS             complement(26810..27982)
                     /locus_tag="VFB53_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN86988.1"
                     /translation="MRAWLAALSLAAAPALAQGPPVVLGVVLPETGLLADLAADMKKA
                     LVLWQEQRNAAGGLLGRRVDLRIADDHSEASAAGRLYARLVDAEGAQLLIGPFGNAAC
                     VVAAAYAERQRRVLVNAAGISRDLHRAGSRYVFQVPAPLADYGAGVLAIARAMGYRSL
                     HVVARNDPGSRESAARLVEQARALGLEPGVAQTVAAGLRDYAAQVAAARARNAEIWVA
                     FGQPEDAAEMVKSFKRIGYAPWVFLAQGAAEPRFLQFVGQDAEFALGLVAYDTRFATR
                     GNAAFVEAWRKRWQGEPGALAAGAYAAALVLEEAVRKSGSLDQERLREALSALDTETP
                     VGRYRVDKTGAQVAARPAVVQILAGRREVVWPRALATAQWRLPYPRWDERQVHAAQ"
     gene            complement(27982..28719)
                     /locus_tag="VFB53_09425"
     CDS             complement(27982..28719)
                     /locus_tag="VFB53_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZN86989.1"
                     /translation="MNPSTHDPALGALPFAAGSVARSVEEAILSRRSLRAFKPDPVPR
                     ATLERLLALASRAPSGTNIQPWNVYVVAGEARERLAAAMHAEFMQHGEEGWKREYEYY
                     PTKWRDPYLARRRKLGWDLYGLLGIGKGEREKTHRQHARNYVFFDAPVGLVVTLERDL
                     PVGAWLDTGMFLQNLMLAARGFGLDTCPQAAIASAHTVLRRVLQIPEPEMVVCGMSLG
                     YARPDAIENTLVTVREPVARFARFFGC"
     gene            28738..>29999
                     /locus_tag="VFB53_09430"
     CDS             28738..>29999
                     /locus_tag="VFB53_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN86990.1"
                     /translation="MDARLQAAFAALVGVKHVLAAPQDTSPYTTDWRRQYRGAALGVL
                     RPASTAEVAAVLGLCARERIAVVPQGGNTGLSGGSVPTGTLPEIVLSLSRLARIREVD
                     TLNDTITVEAGCVLADIQRAAREAGRLFPLSLAAEGSCQIGGNLSTNAGGVNVLRYGN
                     AREQVLGLEVVLPDGRIWDGLRALRKDNTGYDLKQLFLGAEGTLGVITAAVLRLHPSP
                     TASATAWIAVQSPARAVELLAGLRQRLGERISAFELVSRRCLEAVLQHASAVRDPLPR
                     AYPWYVLAEITDSGDDASLAAQVEQAFGACSDLVDDVVLASSASQSQALWKIRETIPE
                     AQFANVKHDVSVPVSRVPDLIERAGRALEAAFAGAPVYAFGHIGDGNIHYNVGDQALL
                     ARRAEVSRVVYDAVAALGGSISAEHGLG"
BASE COUNT         4671 a        10737 c        10291 g         4300 t
ORIGIN      
        1 cgcctcatca gcacgctcaa gcgcctgcgc gacctcggca attcggtgat cgtggtcgag
       61 cacgacgccg aggcgatcct cgccgcggac catgtggtgg acatgggacc gggggccggg
      121 gttgcgggag gccgcgtggt cgccgagggg ccgcccgagg cgattctcga aagtgagtct
      181 tcgctcacag gcaagtacct cagccaccgg ttgcgcattg ccgtgccgcg cctgcgcaac
      241 cggcccagcg ccaagcgcgc cctgcgcatt cgcggcgcca gcggcaacaa cctgaagcag
      301 gtcgagttcg agctgccggt gggcctgttc gtctgcgtga ccggcgtctc gggctccggc
      361 aagtcgaccc tggtcaacga cacgctgtac cgcgcggccg cgcgccacct gaacggcagc
      421 accgacgagc cggcgccgta cgaatcgata tcgggcctgg agcagttcga caaggtggtg
      481 agcgtcgacc agagcccgat cggccgcacc ccgcgctcca atccggcgac ctacaccggc
      541 ctgttcgcgc cgatccgcga gctctacgcc ggggtgcccg aggcgcgcac gcgcggctac
      601 gacgccgggc gcttctcctt caacgtcaag ggcgggcgct gcgaggcttg ccagggtgac
      661 ggcctgatca aggtcgagat gcatttcctc cccgacatct acgtgccctg cgacgtgtgc
      721 cacggcaagc gctacaaccg cgagacgctg gagatccgct acaaggggcg cagcatccac
      781 gaggtgttgc aactgaacgt gcgcgaagcg cacgagttct tcgcggcggt gccggccatc
      841 gcgagaaagc tgcagacgct gctcgacgtc ggcctgggct acgtgcagct tggccagtcg
      901 gccaccacgc tctcgggcgg cgaggcgcag cgcgtgaagc tctcgctcga gctgtccaag
      961 cgcgacaccg ggcgcacgct ctacatcctc gacgagccca ccaccgggct gcatttcgcc
     1021 gacatccagc tgctgctcga cgtcctgcac cgcctgcgcg acgcgggcaa cacggtcgtg
     1081 gtgatcgagc acaacctcga cgtcatcaag accgcggatt gggcgatcga cctcggcccg
     1141 gagggcggcg acggcggcgg gcgcatcgtg gcgcagggca cgccggaaac agtggcccgc
     1201 aaccccggga gccataccgg ccgcgccctc aaacaggtgc tcgaggtgcc gaagaagcaa
     1261 gccgcttgac agttacgaac gttcgtacta ttttatcggc atgaacaagc ccaccaaagg
     1321 cgagcagacc cgggccgcca tcctcgacca ggcggtggac atcgccagtc gcgtgggact
     1381 cgaaggcctc accatcggga ctctcgccga agccaccggc atgtcgaagt ccggcctgtt
     1441 cgcgcacttc ggctcgcggg aaggcctgca gctcgcaacc ctggagcatg tcaaggcgcg
     1501 cttcggtgag caggtgctga cgccggttct gggcgtcgag cgcggcctgc cgcggctgcg
     1561 cgcgctgttc ctcgcctgga tcggctggaa ggccgcgccc ggtcccggcg gttgcgtgct
     1621 gcaatcggcc gcgcacgagt acgacgaccg tcccgggctg atccgcgatt cggtggtcga
     1681 cggccagcgc cggggcctgg cgatgatcga gcgcgcggta cgcgtcgcga tcgaggaagg
     1741 ccacctgcgc gccgaaaccg acccggcgca gatcgcgttc gagatgtacg gccagattct
     1801 cgtcgcccac aaccacagcc gcctgctcgg cgaccgcgaa gggcgcaagc gcgcgctgtt
     1861 tgcgttcgac gagctggtgg cacgccacgc cgcaactccg ccgcgacagc gcgcggcggc
     1921 ctaactccag ggagaactgc catgagccac gccaaacctc tgtctttgaa tagcacgaac
     1981 gttcggtttc tgtttgaaac gctcctgagg cgcgccgggc tgggggcttc tgcgctcctc
     2041 gcccccggcc tcgccggcct gtgggcggaa cggctgttcc tcaccccgcc caagccgcgc
     2101 taccccagca gcgagttctt cgacctgatc gacgcgcgac agagttttct gcgccaccgc
     2161 gggcggcata tcgccagctg gcgctggggc tccccggacg cccccgcggt gctcctggtg
     2221 cacggctggg gcgggcgcgc gacgcagatg cgccgcttcg tggcgcacct caccgccgcc
     2281 ggcttccgcg tcatcgccta cgaccagccg gcgcacggcc tgtccgaagg ccggctcacc
     2341 ggattgccgg acttcgccga cgtgctcctc gcagtggcgc gccaccatgg caaggtgcat
     2401 gcggtgatct cgcactcgct cggcggcccc gcggcggcaa tcgcgctggc gcgcgggctc
     2461 gccgcgtcgc gcgccgtgct gatcagcccg ccctcggacc tggtcggcta ttcgcgccgc
     2521 ttcgcgcgct ggctcgccct cccggaacgc gtgcgccgct cgatgcaggc ggcgatcgag
     2581 gagcgcttcg gcgtacgctg gtcggagctg gagatcgcgc gcgtcgcgcc gctgctgcgc
     2641 gccgaggcgc tggtgatcca cgaccggcag gacacgcagg tgccctggag gcagggcgcc
     2701 gcgctggcgc ggcattggcc gggcgcacgg ctgatgagca cggtcggcct ggggcacgga
     2761 cgcatcctgg aagacgatgc ggtggcgcgc gccgcggtcg atttcatcgc cgggcgctct
     2821 gcggtggcgc aagcggcgtt accatcgctg cccaatcccg ctccgctgct ctgacatgga
     2881 ccgctccctc accaccacct ggtcggaccc tcttgccgca ttcgaggagg gccgcaagct
     2941 cgcgcccatc gagcacctgc gcgcgatccg cgacgggcgc ctcccggacc cgcccatcgc
     3001 ccgcctgctg ggcatgcggc tggtgaaggt cgagccgggc gaggcggtgt tcgagctgac
     3061 cccggcggag cagcactaca acccgatcgg cgtggttcac ggcggcgtgg ccttcacgct
     3121 gctcgattcg gcgatgggct gctgcgtgca gaccaccctg cccgcgggaa agggctacac
     3181 cacgctcgag atgaaggcca acctggtgcg cgcggtgacg ctgcagaccg gactgatccg
     3241 cgccacggcg cgcgtcgtgc acgccggcaa gaccacggcc accgcggagg cgcggctgga
     3301 agacgccgcg ggcaagctct acgcccacgg cacgagcacc tgcctgatcc tcgcctgagg
     3361 ctgtttacag gcggggagga atggggcgag aatggccgcg ccccccgatt ccgcatgccc
     3421 ggcaagaact tccgcttcgg gccgaccccg gagcatcgcg cccagcacct gttcgtgttc
     3481 ctgcacggct gcggggccac ttccaagtcc atgatcccga tcgcgttccg cttccaggcg
     3541 cgctttccca gcgccgccct ggtggtgccg agcggcttcg cctccgccga ggtcggcggc
     3601 gaggcgcagc agtggtactc gagccgcggc ctgaccgacg agaaccgcct cgagcgcgtg
     3661 gccgccatcc tgccgcggct ggaggacctg gtgcggcgcg agcagaccaa ttgcggcgtg
     3721 ccgatggaac gcaccgtgct gatcggtttt tcccagggcg gcacggtggc gctggaggcg
     3781 gtgaagcagc tgcccggcct cgccggcgcg gtggtggcct atgccgcgcg cttcgcccgc
     3841 ctgccgcagg acggcgcgcg cctcggctcg cgcattcacc tggtgcacgg cgagtacgac
     3901 gcggtggtgt cgcgcgtgca ctcggagcgc gcggcgcgcg tgctcgccgc gctgcacgtc
     3961 ccggtcacgc tcgacatcgt cgaccagctc ggccatgcgc tctcgcacca cgcgatctgc
     4021 ctgggctcgc tgcgcctgct gcaggggatc tacgaggggc gccgggccac gctgcattga
     4081 agcaggccct catcctgttc gcgcacggtt cgcgcgatgc gcaatgggcg cagcccttcg
     4141 aggcgctgcg cgccgcgctc gcgccgcact gcgacgtcga gctcgcgtac ctggagctca
     4201 tggcgccgac cctggaggaa gccgtggcgc ggctcgcggc gcgcggcgcg cgccgggtgc
     4261 gcgtggtgcc ggtgttcctc gggcagggcg cgcacctgaa gcgcgacctg ccggcgctcg
     4321 ccgcggccgc cggcgcgcgc cacccgggac tgcagatcgc gctcgagccc gccatcggcg
     4381 agcggcccga ggtgattgcg gccctcgccg ccgccatcgg gcgcggctag aatcgcaccg
     4441 catgggaacg gtggtggctc tgggaggtca ggtcggcggc gccaagctcg ccgagggcct
     4501 gtaccgcctg cgcggcaagc gactcgcggt gatcgtcaac accggcgacg atttcgagta
     4561 cctgggcctg caggtttcgc ccgacatgga caccatgctc tacgcgctcg ccggcatcgc
     4621 cgacgccgcc aagggctggg agcccgcggg cgagactttc gcctgccatg gcatgctggc
     4681 gcggctgggc gccccggccg aggtgccgct cggcgaccgc gcgctcgcct tccagctgct
     4741 gcgcgccgaa gcgctgcgcg ccgagcgccc cgccaccgcg atcgcgctcg agctgtgcca
     4801 gcgcctcggc atcgaggcgc gcgtgctgcc gatgagcaac gatccggtgc gcaccatcgt
     4861 caataccgag ctgggcgcga tgagcttcca ggactatttc atggagctca agtgcgagcc
     4921 cgcggtgcgc agcttccagt acggcggcgc cgatgaggcg cgcctgtccg acgaggtgct
     4981 cgacgcgctg catgcgggcg atctggaggc cgtggtgctg tgcccctgca acccctacca
     5041 cagcatgcgg ccgatcctcg ccgtacacgg catgcgcgag gcgctgcgcc ggcgcggcgc
     5101 cccggtgatt gcggtgagcc cgatcatcgg cggccgggcg ctgcagggat cggcggcgaa
     5161 gatgatgcag gagctggggc gcgaagtttc gccccgcgcg gtggcaatgg agtaccacaa
     5221 gctgatcgac ggcatcgtca tcgacagcgc agacgaggcg ctggccgagg gcctgcgcgc
     5281 cagcggcgtc gaggtgctgg tggcgcagac ctacatgcgc actgccgacg accggctgca
     5341 gctggcgcgc gcggtgctgg agttcgcgcg cgccgtgcgc ctgcgcaagg aagtcgccgc
     5401 cgacgcctga ggcgtgccgc gggcacgccc ggcgcccggc gcttccggca tgcgcaaaga
     5461 cagcctgcag tgcctggggc cgcacggctt ccatcgcatc gcctataccg agtggggcga
     5521 ccccggcgcc gggcgcgtgg tggtgtgcgc gccggggctg acgcgcaatt cccgcgattt
     5581 cgacttcctc gcgcagcgcc tggccgagcg ctgccacgtg atctgcatgg acgtcgccgg
     5641 gcgcggcgcc agcgactggc tgcccgacaa gtccgactac ggcttcgacc tgtacgaggc
     5701 cgacggcgcc gcgctcattg cacgcgccac cgcacggccg cgccggccgc tgttcgcctg
     5761 gctgcagcgc gtgctgccgg agcgcgctgc cgaggtggcc tgggtcggca cctcgatggg
     5821 cggcatcatc ggcatgctgc tcgccgcgca gcccggcagc ccgatccgcc ggctggtgct
     5881 gaacgacgtc ggcgccaccg tcccctggag cgccctgatg cgcctcaagg gcctggtgcg
     5941 ccgccacgag ccctacgcca acctcgcggc cgtggcgcgc cacgtgcgcg aagcctgcgc
     6001 cggcttcggc ccgctcaccg attcgcagtg gcgccacctc gcgcgccaca gcgtcacgcg
     6061 gctcagggaa ggcggctacc gcctcgccta cgaccccgcg atcgaggcct cgctgtggaa
     6121 cggctcggac ctcgacatgc cgatcggacc cgagctgatg cgcggcatca acctgtggcg
     6181 gcactgggac gcgatccgcg cgccgacgct gatcctgcgc ggcagcgaat cggacgtgct
     6241 gcccgcagac gtggcgcgcg acatgctggg ccgcgggccc aaggcccggc tggtcgagtt
     6301 cgcgggcgtg ggccacgcgc cggcgctgat ggccgccgac cagatcgacg ccgtcgaggc
     6361 gttcctgctg gattgatcag cgcagcagcg cgtactcgcc gcccctgtcg agggcgcgac
     6421 ggtaggcggg gcgtgcgtgg atgcgctcga ggaaggacat cagccgcggg cgcttcgcgt
     6481 ccagcccgcc gcgggcggca gccgcctcca gcggaaaact gatctgcacg tcggcggcgc
     6541 tgaactcggc gccagcgaac cacgcgctct tcgcgagctc gccctccatg aaatcgaggt
     6601 tgcgctcgat gttgggctgc acgaagccgc tcttggcgcg tcgcgcgatg gcgcgcgcca
     6661 ccggccgcac gaaaaagggc atcggcccct tctcgatgcg gtcgaagacc agcttcagca
     6721 gcagcggcgg catcaccgtg ccctcggcga aatgcatcca gtagatatag cgcaggcgct
     6781 ccggcgtgcc ggcggcgggc gccaggcgcc cgccgccata ccgctccacc aggtactcga
     6841 tgatcgcgcc ggattcggcg agcgtcgccg cgccgtcctc gatcaccggc gatttgccga
     6901 gcggatgcac cgcgcgcaac tcgggcggcg cgagcatggt cttggcgtcg cgctggtagc
     6961 gcttcacctc gtaggcaacg cccagctcct ccagcagcca cagcacgcgt tgcgagcggg
     7021 agttgttcag gtgatgcacg atgatcatgg cggccgcctc agctctgcag ttcggggatg
     7081 tcgcggaaca gctccagcgc ctccgggttg gcgagcgcgt cggtgttgcc caccggcatc
     7141 ccgtgcacca ccgccttcac cgccagctcg accaccttgt tgttcttggt gcgcgggatg
     7201 tcgggcacct gcacgatctt cgacggcacg tggcgcctgg tggtgtgctc gccgatctgg
     7261 cggcagatcc tctcctgcag gccggcgtcc agccgcaggc cttcgcgcag cttcacgaac
     7321 agcaccacgc gcgtgtcggt gggcttgtcc ggcggccaca gctggccgat ggccaccgac
     7381 tcctccacct cgggcacctt ctccacctgg ccgtagatct cggcggtgcc gatgcgcacg
     7441 ccgcccggat tgagcgtcgc gtccgagcgg ccgtggacga tcatggtgcc gcgcgcggtc
     7501 agctcgcacc agtcgccgtg gcaccacacg ttcggatatt tcgcgaaata cgcggcgtgg
     7561 tacttctcgt cgccgcggtc gttccagaag ccgagcggca tcgagggaaa ggtccgggtg
     7621 cacaccagct cgcccttctc gccaacgatc gagccgccct ccgggtcgaa cacctccacc
     7681 gccagcccga gcgagcggca ctgcagctcg ccgcggtaca ccggcagcac ggcgttggca
     7741 tccgcgaagg ccgccatgat gtcggtgccg cccgaaatcg aggacaggca cacgtccgcc
     7801 ttcacgtcgc ggtacacgta gtcgtagctc tccggcacca gcggcgagcc ggtggagacg
     7861 atcatgcgca ggcccggcag gggatggctt tcgcgcggct tcaggccgcg cttcgcgatg
     7921 gcatcgatga accgggccga ggtgccgaaa tggctgaagc gctcgcgttg cgcgtaatcg
     7981 aagaggatcc tgccgtcgcg cgcgtagggc gagccgtcgt agagcatgag cgtcgcctcg
     8041 gcgcacaggc cggcgaacag caggttccac atcacccagt tgcaggtggt gtagtagaag
     8101 aaatgatcgc ccgggcgcac gtcgaactgc agcttgtaca tctttaggcc ggcgagcagc
     8161 gacccgcccg ccccgtgcac gatgcacttg ggcttgccgg tagtgcccga ggtgtagagg
     8221 atgtacgccg ggtggtcgaa cggcagctgc gcccagggaa tgtcgccggg cgtatgccgg
     8281 cgcagccact cctccagcag caccgctttc gggatatcgg agatgtccgg gcgcgcgtcg
     8341 aggtagggca ccaccaccac cttgcgcagg ctcggcagcc gctcggcgat ctcgcgcacg
     8401 cgctcgagca cgtcgtgccg cttgccgttg tacagatagc cgtcggcgac gaacaggatc
     8461 ttgggctcga tctggccgaa gcggtcgatc accccgtcga cgccgaagtc cggcgagcag
     8521 gacgaccaga cgatgccctg cgacagggcg gccagcgcga acatcgccgt ctccggcaga
     8581 ttggggatga acgccgcggc gcggtctccc acgcgcaggc cgagctgctg cagcgcctgc
     8641 accgcgcgcg acacgtcgct cgtcaggtcg gaatagctca cccgccgctg cgcgccgcgc
     8701 tcgtcccaga acaccagggc gtcgccacga tcgccgcggc gcagcaggtt ctcggcgaaa
     8761 ttgaggcgtg cctcggggta ccaatgcgcc cccggcatct tgtcgccgtc caccagcacc
     8821 gtgccgccct tgcgcgaggc gcgcacgccg caccactccc acacctcgtt ccagaacagc
     8881 tccgggttgt ccaccgtcca ctggtagaac tcggggtagg tgttgaggtc gagccggtgc
     8941 ttgcgcaccg cgtggcgcgc gaactgcgtg agctgcgcct gctcgatcgc ctcctccgac
     9001 ggcgtccaca tcggttccat gggcggatta tccctcggcc gcggccagcc tgaggcgagc
     9061 ggttcaccgg caagcggaaa tatccgggta agatgcgccc tcttttttcc tcccgccccg
     9121 cccatgccca agcccgccta cgactgcgcc aagtgtcccg cctactgctg cagctacccg
     9181 gaaatcgagg tgtccaaggc ggacatcgcg cgcctggcgc ggcatttcgg cgtggatcaa
     9241 gcgctggccg aggagcgcta taccaagctc gagcggcagg aggaggtgcg tatcctgcgc
     9301 caccgcgagg acgagcactt cacttcggtg tgcatgttcc tcgaccgcaa gtcgcgcagc
     9361 tgcacggtct acgcgtcacg tccgtacacc tgccgcggct acccctacgc cgcgcgctgc
     9421 ggctactacg aattcctcaa gttcgagcgc gaacaccagg ccgacgagag cttcgtcgcc
     9481 acgacctgac tacttgcggc gctcgagcgc ctgcttgcgt atcgcgctga gggtggcggc
     9541 ggtggtgatg gcgtccggat cgatgcgcag ctcgatcagc gccggcttgc ccgaggcaag
     9601 cgcgcgctcg aaggccggcg cgaactgagc ggtggcctcc accagctcgc cgtgtccgcc
     9661 gaaggcgcgg gcataggcgg cgaaatccgg gttcttcaat tccgtccccg agattcttcc
     9721 cgggtattcc cgctcctggt gcatgcggat ggtgccgtac atgccgttgt tcaccaccag
     9781 gacgacgatc gccgcgccgt actgcgcggc ggtggccagc tcctggccgt tcatcaggaa
     9841 gtccccgtcg cccgtcacgg ccaccacggt gcgctgcggc gcagcgagct tggccgccac
     9901 cgccgccggc agcccgcacc ccatcgaacc gttggtcgac ccgagttgcg tgcgaaagcc
     9961 cttgtagcgg aagaaacggt gcacccagcc ggagaaattg cccgcgccgc cgcacatgat
    10021 ggcgtcttcc ggcagccggt ccgacagcca cttcacgatc tgcgcatact gcagttttcc
    10081 ggggatgggc tgcggctcgg tccaggcgag gtactcatcg cgcgcctgcg cggtgcgcgc
    10141 agcccatcgc gcgccgtcca gcgccacgct cgccagcgcc tcgacgaacg ccgccatgcc
    10201 cgagttgatg ggcagcgcgg cgtggtacac gcgcccgagc tcttccgcgc cgccgtgcac
    10261 gtggatcagc gtctggcgcg gcaccgggac ctcgaacagc gaatatccgc cggtcgtcat
    10321 ctcgccgagg cgcgcgccga tcaccagcac cacatcggcc tcgcgcacgc gctgcgcgag
    10381 cttcgggttg atgccgatgc cgacgtcgcc cgcaaagctc gcgctgcgat tgtcgagcag
    10441 gtcctggcag cggaacgaag tgcccgccgg cagccgcgag gcttccagcc aggcgcgcag
    10501 gccgtcgcag gaggcgcggt cccagccgcc gccgccgatg aggacgaagg gccgctcggc
    10561 gccctgcagc agcctgcgca gttcgcgcag gtcggcggcc gccggcgccg cgcgcaccgt
    10621 cttgtagcgc ggcgcatcgg ccaccgccgc ctcggcgaac agcatgtctt ccggcaaggc
    10681 cagcagcacc gggccggggc gacccgccat cgccgtgtgg aaagcgtggc tcacgtactc
    10741 ggggatgcgc tcggtgcggt cgatctgcgc cacccacttg gtcatcggcc catacatgcg
    10801 ccggtagtcg atttcctgga acgcctcgcg ctcgacgaag tcgttgccca cctgcccgac
    10861 gaacaccacc agcggcgtgg agtcctggaa cgcggtatgc acgccgaccg cgccgttggc
    10921 cgcgcccggg ccgcgcgtca cgaacagcac gcccggctgc ccggtcagct tgccgtaggc
    10981 ttccgccatg taggacgcgc cgccttcctg gcggcagacg atgaatttca gcttgtcggc
    11041 gacgtcgtgg aagccgtcga gcaccggcag gtagctctcg ccgggcacgc cgaaggccat
    11101 ctgcacgccg tggccgagca gcgcgtcggc gaggatgcgc ccgccgtgac ggactttcag
    11161 gtcggatttc atagcagcgc ggcggcgtaa gcccctgcac cgagcgtggt gcacacgaga
    11221 atccagcccc atggcacggg atgcgcctcg accagccggt ccagggtcag gttgaggtca
    11281 tcggcgccac gcatcgcgcg gcgggaagaa atccaccgat tggcatgctt ctcgatgctg
    11341 gtcagggcat ccggagaaaa gcggagcagg aatccgaccg cgaccccggc gacgctgccc
    11401 acgaccagga accatttcag ggccgccgcc gcaatggcga cgatgcgcac catgtcgccc
    11461 tttgcgaatg acgcgccgac cgcggccgca tcgacgccgg cgatcagccc gaaagcggaa
    11521 aatgcgcctc ccccgatcag agcatagccg agcaagcggc gatgcccatg cgagagcagc
    11581 tcggtgtccc gcgggatctc catcggcttg agggcgccgc gcatggagat ccagcggttc
    11641 atcgcgtgga agaatcgcag ggttcgcgcg ctggaaacga tcaagccgat gcccacgacg
    11701 aaaccgacgg cggagaatac cagcaccacc acgacgaaga actggtggag cagctgccag
    11761 atcaggttga attgggacgg catgcgtcct ccatcgtcca ttggctgcgc agcttctcgc
    11821 agcattcggc caccgcggcg ttggccgtgt cgagcacgcc gcccatcagg atgaagccgt
    11881 gcaccatgtc ggggtactcc cggtaggcga cgtccacgcc ctcccgcgcc atgcgctcgg
    11941 cgtactcgcg cccctcgtcg agcaagggat catagcccgc ggtgatcagg tacgcaggcg
    12001 gcagtccagc cagggacttc gccagcagcg gcgatgcgcg ccagtcggcc aggtcctcgc
    12061 gccgcggcag gtactgcgcc tggaagtagt ccatcgactt tttggtgagc aggtatccct
    12121 cgccgttgcg cgccaccgag gggtgctgcc cgcgctggtc ggtcgccggg tagatgagga
    12181 gctgataggc gatcgccggg ccctgcgcgt cgcgcgccag cagcgccacc accgtggcga
    12241 ggttgccgcc ggcgctgtcg ccgcccacgg cgacgctcgc gtggcgcgag gcgacgaact
    12301 tcgttgcggc aaggcaatcg tcgaccgcgg cggggaacgg atgctccggc cccatgcggt
    12361 aatccacgga gtacaccgcg cagcgcgcac cgttcgccag ctgccggcag agcacgtcgt
    12421 gcgtgtccag atcgccgatc acccagccgc cgccgtgaaa atacacgagg gccggcagtt
    12481 tctcctcggg cttcgacccc gccggccggt acgcccggag cgccaccggt ccgggggcga
    12541 tcacgaggcg cgcctcggcg acttcgggcg ccctgggcgc cagcgcggcg cgcgtgtcgc
    12601 ggtaaatgcg gcgcgcgacg tgcgcgggaa cttcgtagta gggcggcagc ggcgacttgg
    12661 ccgcccgctc gagcaattgc gcaacctgcg ggtgcagact cacctgaccg cggcgagcat
    12721 cttcttcagg cgctcgacga agaacggcgc gcacttgtgg atcttcgtct cctgcgtgac
    12781 gatcagcggc cggatgcggg gcaggtaggc ctgccactcg gggtcggcct gcatcgaggc
    12841 gcgcatcttc tcgcgctgca gcaggtcgtc gtaggcccac atgtgcgtca ccaggttctg
    12901 catgccggcc tcggtgacgt aatagccgac caggttcggc aggtgccgca gctgcacctt
    12961 catgcccact tcctcgtagt gcttgaagta ctccggcgcc ttgccgacct gcaggagata
    13021 catgcgctct tcgacgtaca tggggctcct ccctgggatt ggtcgattat agtagcgggg
    13081 aaatggacga catcaacacg ctgcgccgca tcctttcgca gaaccgcgtg atcgcggtgg
    13141 tgggcctctc ggccgactgg ttccgcccga gctatttcgc cgccaagtac atgcaggagc
    13201 acggctatac ggtgatcccg gtcaatccgc gctatgccga ggtgctgggg cagaagtgct
    13261 acaagtcgct gcgtgaaatc cccggcaagg tggacctggt cgattgcttc cgcaagaccg
    13321 aggacatcct gccgatctgc gacgatgcga tcgccatcgg cgcgaaggtg ctctggcagc
    13381 agctcggcgt gaggaacgaa accgccgctg ccaaggcgcg cgccgcaggc atggacgccg
    13441 tgatggaccg ttgcgtgaaa atcgagcacg ggcggctgtt cggcggcctg aactgggtcg
    13501 gggtcaacac ccgggtcatc tcggccaagc gcccgcgctg gctcgcgtac tgattcgtcc
    13561 ccgcggggcc tcgggcaggc atctgttttt ccgatgtttt agctgcgatc tgccactagc
    13621 gcgcgctggc aggcgacgtt atcctagcgt ccatgcctcg ccccatccgc atctttgaag
    13681 gccgcttcgg tcgcctggtg ctcgccgagg cggctgcgcc cggaatacct gcggcgcagg
    13741 gcgagccgct gatcctgctc aaggtggatg gcgccgacag cgtcgtttcc atcggcggaa
    13801 cgccccagcc gctcacgcgc gaggatgcgc tgttcgtgaa tcccggcgag gcggcccaga
    13861 ccatgggcag cgccaacggc gcgcccgcac gcgtcatcgc ctactacccg tccaccgcat
    13921 ggctgcaggc gagtttcccg gcagccttcg aggacgccgg cgccccgatg ttcccccgcg
    13981 cgcgtgaagc catcgctccc cgcatgcggc aactcgccga caccctggcg gtggagaccc
    14041 tgaacgacca gttcctgtcg cccgagcggc tcgaattcat gctgcaggag atgatgctgt
    14101 cggtgatcga ggcctacatg gcgcgccggc gcgccggcgc gtcgctctgg cgcggttcgc
    14161 gcttcgccga cacgcgcatc cgcaaggcgc tggcgctgct gcgcgctcgc cccaacaagg
    14221 acgtgaacat cgacgacctg gcgagccagg tcgggctgtc gcgctcgcgc ttctacgacc
    14281 tgttccaggt gtgcacgggc cgctcgccgc ggtcctacct ggacctgctg tgcgtggaga
    14341 gcgcgatcgc caagctctcc agcggccgca cgcggatcgc cgacgtgtcg gcggagctgg
    14401 gcttctcggc gcagagcaat ttcacgcgct ttttcctgaa ccaggtgggc atccccccct
    14461 ccgagtaccg gcgcgcggct tcccggccgg agaccagcga cacccctcca gagaccgcct
    14521 cgcgaggttg agcgccggcc gggcctgcgc gccctgcccc gcagcccgcg gcggcgcacc
    14581 gcgtggagcg atgaacgatg caagacgcca aggcgctgta cctggacctg ctcaagcgct
    14641 gcctcgccaa tacgatctac caggactccg gcacgaccct gggcaagcac ctgcccttcg
    14701 acctgggtca ccgcatcgag ggccgcgact ggcccgtggt cgcgcacacc atgatcggcc
    14761 tgaagcgcct cgatgcgctg catttctgcg tccaggacgt gctcgcgcgc ggcgtgccgg
    14821 gcgacctcat cgagaccggc gtctggcgcg gcggcgccac catcttcatg cgcggcgtcc
    14881 tcaaggcgca cggcgtcacc gaccgcgtgg tctgggtggc ggactccttc gagggcctgc
    14941 cggcgccgga cgcggcgaag tatccggcgg acgccaatta caatttccac gcctttcggg
    15001 agctggcggt gtcgctcgag caggtgaagc agaatttcca gcgctacggg ctgctcgacg
    15061 accaggtgcg cttcctcaag ggctggttcc gcgacacgct gcccggcgcg cccatccgca
    15121 gccttgccgt cctgcgcctg gacggcgacc tgtacgaatc gaccatcgat gcgctcacgc
    15181 acctttaccc gaaactggag cggggcggct acgccatcat tgacgactac agcgacgtgc
    15241 cggcctgcaa gcaggcggtg acggaatacc gcgccgcgca cggcatcacg gaggagatcg
    15301 tccccatcga ctggaccggc gttttctggc gcaaggaacg ctaggctaga atccggcacc
    15361 ctggaggagg aatggtcatg cagcaacgaa gactcggcag cggcgcgctt ttcctgacga
    15421 ttgcgcttgc aggctgcgcc ggcctctccg gcgacgcgtc ccggcaggaa ctgctgccca
    15481 ccggcaagct gcgcgtcggc atcggcgtcg gcgcctcgcc ctccgcgttc tgggcggtga
    15541 aggaccccgc caccggccag ccgcgcggcg tcacggtgga cctcgccaac gcacttgcgc
    15601 gcaagctgaa cgcgccgctg cagctggtgg tctatgccaa ctccggcgaa gtggtggccg
    15661 ccggcccgaa gggcgaatgg gacgtcgcct tcatgcccga ggacgccgag cgccgcaaga
    15721 tggtggactt cggcccggcc tactacctgt ccaccagcac tttcctcgcg cgcgcgggct
    15781 ccggcatcaa cacgctcgcc gacgtggatc gccccggcgt gcgcgctgca ggcatcgcca
    15841 acaccacgac tgcccgcacc gccatcagca ccctcaagca gaccaagctc atcagcttcc
    15901 gcaccgtcga cgaggtgatc gccaagatga aggcggggga aatcgacgcc atcgccctgg
    15961 ggcgcgagtc gctgcagggg ctcgcgccgc agatccccgg ctcccggatc ctcgacggcc
    16021 atttccacgc cgccggcacg gcgatcgccg tgccgaaggg caagccggcg gcgctggcct
    16081 acgccaccgc cttcatcgag gaggcgaagc gcgacggcac ggtgcgcggg gcgctggatg
    16141 gcgtcgggct gcgcaacgcg ccggtcgcgc ctgccgcgaa gtagcgccgc cccgctagaa
    16201 tcgcgcatgg ccgaccgcaa gttcgggttc gagacgcttt cgctgcacgc cggccagatc
    16261 cccgaccccg ccaccggggc gcgcgccgcg ccgatctacc agaccacatc cttcgtcttc
    16321 gattcggcgg accacgccgc gagcctgttc aacctgcaga cgttcggcaa cgtctactcg
    16381 cgcatctcga accccacggt ggcggtgctc gaggagcggg tcgccgcgct ggaaggcggg
    16441 cgcgcggcgc tcgcctgcgc caccggccag gcggcggaag ccacagcgat cctgacgctg
    16501 tgcaaggcgg gcgaccacgt ggtgtcggcc tccaccctgt acggcggcac gcacaccctg
    16561 ctcggcgtca acctgaagaa gctgggcatc gacaccacgt tcgtgcatcc ggacgagccg
    16621 gagaatttca ggaaggcgct caagcccaac acgaagctgc tctacgccga gacgctcggc
    16681 aacccgctga tcaacatcgt ggacatcgcc gcggtggcgg cgatcgcgaa agacgcgggc
    16741 gtgccgttcc tggtggataa caccgtgccc tcgccgtacc tgtgccggcc gatcgagcac
    16801 ggcgccgacc tggtggtgca ctcggccacc aagtacctgg gtggccacgg caccaccatg
    16861 ggcggggtga tcgtcgaatc cggcaggttc ccctggggca acggcaagtt ccccgaattc
    16921 accacgccct cccccggcta ccacggcgtc atcttccacg agaccttcgg cgacttcggc
    16981 ttcaccatga aggcgcgcat ggagatcatg cgcaccttcg ggccgacgct cgcgccgctc
    17041 aatgcctggc tgctgctgca ggggatcgag acccttggcg tgaggatgga ccgacactgc
    17101 gccaacgcgc tcgcggtggc gaagttcctg caggggcaca aacaggtggc gtgggtgaac
    17161 taccccggcc tgcccgacaa caagtactac cggcttgcgc aaaaatacct gcccaagggc
    17221 gcctcgggcc tgttgaactt cggggtgaag ggcggtgcga aggcaggcga gaaattcatc
    17281 gaggcggcgc agttcatgag ccacctcgcc aacatcggcg acgcgaagac cctcatcatc
    17341 cacccggcct ccaccactca ccggcagatg agcgacgagg agcagctgaa ggcgggcgtc
    17401 acccccgaca tggtgcgcat gtcggtgggc atcgagacgc tggacgacat actctgggac
    17461 atcgaccagg cactcaagga ggcggggaaa gcatgatcag ctaccaaatc atcgagcacg
    17521 gcaaacccct gcagaaggtg atgcacgaaa cccccgtgcc caagggcacc gaggtgctgg
    17581 tgcgcatcac gcgctccggc gtgtgccact cggacgtgca catctgggac ggctacttcg
    17641 actggggcgg cgggaaacgc ttctacgtga aggaccgcgg ctgcatcccg cctttcaccc
    17701 tggggcacga gcccttcggc gtggtggcgg cggtgggtcc gcgcgcgaaa ggcgtgaagg
    17761 tcgggaagaa gttcatcgtc tatccctgga tcggctgcgg caagtgcccg gtgtgcaagg
    17821 cgggacagga caactactgc atgaaggcgc agttcctcgg cgtgatgaag ccgggcgggt
    17881 actcttcgca cgtcctcatc ccgcacccga agtatctggt ggatgccgcc ggcatcgacg
    17941 agggcttcgc cgcgacgctc ggctgctcgg cgatcaccgc ctacagcggc ctgaacaagc
    18001 ttcccaggct cgggcccaag gactggatcg cggtcatcgg ctgcggcggc ctcggcctcg
    18061 tcgccctctc gatcctcaag gcgaaggggg tgaagaacgt gatcgcctgc gacgtggacg
    18121 aggcgaagct cgccgcggcg aggaagcagg gcgcgaagct cacgctcgac acgcgcgcgc
    18181 cggacgcggc gcagaagctc ggcgccctgg cgatgggcaa cctcgccgcc tcgatcgact
    18241 tcgtcggcat gcctgccacc gcgatgctcg gcaccgccgc gctgaggaag ggcgggcgct
    18301 acgtgatttg cggcctgtac ggcggggagc tggtgcatcc cctgccgccg atcgcccagc
    18361 gcgccatcgg catcgtcggg tcctacgtgg gaaacctgca ggaattgaaa gaggtcgtgg
    18421 cgctggcgaa gaagaagaaa ctgaaggcca cgccggtgga gctgcgccgg ccggaagacg
    18481 tgaaccgcac gctggacgag ctgaaggcgg gaaaggtgct cgggcgagtc gtcctggatt
    18541 tcgaagccgc cggctaggcc gccgccaccg gcggcatcgg gcaatccagc gcggccgaca
    18601 ctgcgcgcag cagttccgcc tccgcgggcg cgagcttggc gtcggcaagc gaggcgcacg
    18661 ccagcgcctt gaggagccgc ggcttctgca aggggcgcag ccggcgcagg cgcgccagcg
    18721 ccccctggat tccctccagc gtcaaggcct tcaccgggat cggttgcatc gcgctgccga
    18781 gttccttgca gcccgccgca aatgcctggg ccggattcgc gccgccggcg tgcgcgacca
    18841 gcgagagcat caggcgggtg tcatcaacca gcgcatccag cgacccaagc tccggctcgc
    18901 cgcccgcacg cccgggctcc tgcagggcgc tcgccaggat gacttcgagc acgaactcct
    18961 ccagcgtcat gcgacggtcg gcggcgatca tggcatgcag gcgggcgagg aaattccgcc
    19021 tttcgtcgcc cgcgagagcg cgcagcgccg ggatcgccag ttccacgagc ggcagccgcg
    19081 ccgtgcggcc cagcgccgcg atcgattgat gcacggcgac gacggcatcc gcccgcgcgc
    19141 cgagcatcgc cacctgggcg tcccgcgcct ctccggccgg cgcgagcacc agcgcgcaca
    19201 tcgcgaccgc ggcgccctcg ggcgcatgca gcgcctcgcg cagcggcgcg ggcagcgact
    19261 ccaggaaggc gctcgcgtag gcgacttgcg gccgggaaat cgtgccgatc gagtcggcca
    19321 cgcccgccgg cggcgcagcc ggctccgcgg cagcggccgc gggccgcgac gagacgcgct
    19381 tcacgacctc gtccagcgtg acgcggcgac cgtagatgtt ggccaggcgt tgggcgaccg
    19441 tcggatgtgt ggcgaagcga ccggcgcggc ccccttccag cgcgtcgccg aagaacatgt
    19501 ggctcgcctg ctcggcccgc caatggtgga tgcggccgcg ctccgcgtgg cgttcgagtt
    19561 tcaccagcgc gccggcaatg ccgtcggtgt cccgcgtgaa ctgcaccgcg caggagtccg
    19621 cgagcagctc gcgctggcgc gacacgcccg cccggatcaa ccgcgccatg aagctgccga
    19681 gcgagcctac cgcctgcagc agcgcgccgc cgagcacgta ggccaggttg cctttgggct
    19741 tcaggccccg gtcccgctgc ctttgcacgc tttggtcctc gcggtcgagg agcgcctgtc
    19801 cggcgtgcca cagtttcagg atgccgccca gcaatccgat caggcgcatg ttgatgcgga
    19861 tgtccccatt caggatgtgg ctgaactcgt gcgcgatgac gccctgcagc tcgtccctgc
    19921 gcagttcatc cagcaggcca cgggtgacga tcaggacggc atcggcagtg gagaatccgg
    19981 cagccagggc gttcaccgac tgctcgccgt ccatcacgta gacgcgcggc acggccattc
    20041 cggcggcgat ggccatctcc tccaccacgt tgcgcagacg ctgctccgcg aggtcgccgg
    20101 tacgcggttc caacgggcgg gcgcccaagg cttccgcgag cgcggcgcca ccctccgcga
    20161 cggcccggat cgagtcccag gtacccagcc cgatcaccca cagggtggcg agcgacacgg
    20221 caaggaagaa cagtgccccc ggccacgccg acaagtcgtg caagaagagc ttgaccaccc
    20281 accaggcgaa cagcgcagcg caattcgccg ccaggacgat gccgagcatc gctggaacga
    20341 gcagccacag caggcggcgg ctggtgcggc gggcggcttc ctggcgctcg aagaagttca
    20401 tcctgcaaac cttcccgtac caattcccat cggacagtcc cgaggcatcc tagaatacgc
    20461 gcctcggcac accctcagaa tcatgacacc cttttcctgg aacaacccct acgcctggcc
    20521 gcgcaagccc gtcctcgcga agaacatcgt gtccacctcg cagcccctgg cggcgcaggc
    20581 cgggctgcag atgctcgccg ccggcggcaa cgcggtggac gccatcctcg ccagcgccat
    20641 cacgctgacg ctggtcgagc cggtgtccaa cggcatcggc tcggacgcct acgccatcgt
    20701 ctgggacggc aaggagatgc acggcttaaa cgcctcgggc cgctcgcccg ttggctggac
    20761 gcccgagtac ttcaagaacg aaaagcagat gacgcagcgc ggctggaaca gcgtcaccgt
    20821 gccgggctgc atttccgcct ggcgcatgct ctccgagaaa ttcggcaagc tctccttcga
    20881 gaagctcttc gaggcggcga tccgctacgg ccgcgagggc ttcctcgtct cgcccaccat
    20941 cgccggccag tgggcgaagc aggtgccgga actgaaggcg cagccgggat tcgccgaagc
    21001 cttcatgccc aacggccgcg cgccgcagcc gggcgaggtt ttcaagttcc cggaccacgc
    21061 ggcgacgctg gaagcgatcg cgaagaccaa gggcgaggcc ttctacaacg ggccgctcgc
    21121 cgagcggctg gagtcgcacg cgcgccagaa cggcggcgtg atgaaagcct ccgacctcgc
    21181 cgcgcacaag tcggactgga tcgcgccgat caaggttgac taccgcgggt acacgctgca
    21241 cgagatcccg cccaacggcc agggcatcgt ggcgctgatg gcgctgggga tgctgcagca
    21301 cttcgagctg aagagccacc cggtggacgg tcccgacagc ctgcacctgc agatcgaggc
    21361 gcagaagctc gcgttcgcgg acgcctgggc ttacgtggcg gacctcgacc acatgaaaga
    21421 ggtgaagccc gcgcagctgc tcgatgccgg ctacctgaag gaacgcgcga agctcatcga
    21481 cccgaagaag gcgcaggact tcggccccgg caagccgccc agggggggca ccgtgtacct
    21541 cgccgcagcg gacgcctccg ggatgatggt gtcctacatc cagtcgaact acatgggctt
    21601 cggctccggc gtggtggtgc cggggaccgg catctcgctg cagaaccgcg gcggcacttt
    21661 ttccctgaag cccggccatc cgaaccaggt gggaccggga aagcgcccct accagaccat
    21721 catcccgggc ttcctgacca aggacggcaa gccggtcatg agcttcggcg tgatgggcgg
    21781 caccatgcaa ccgcagggcc atgcgcaggt ggtggtgcgc atcgccgact acggccagca
    21841 cccgcaggcc gcctgcgacg gcccgcgctt ccgcttcgtg cagggcatgg acgtgagcgt
    21901 ggaggaaggc ggcttcaagt cctcgacgct ggaagagctg cagaaacgcg gccaccgcat
    21961 cgtcaacgtg gacgactaca accagttcgg cagcgcgcag ctgatctgga agaccgaggg
    22021 cggctacatc gccgccagcg atccgcgccg cgacggccag gcggcgggat tctgaccatg
    22081 ctgaagatct ggggccgggt caattcggtc aacgtgaaga aggcgctgtg gtgcgtgggc
    22141 gagctcggcc tgccgcacga gcgcgtcgac gcgggcatgc agttcggcgt gaacaatacg
    22201 cccgagtacc gcaagatgaa ccccaccggc ctggtgccca ccatcgacga cgacggcttc
    22261 acgctgtggg agtcgcacag catcgtgcgc tacctctgcg ccaagcacag catgggcaag
    22321 ctctgcccgt ccgagctgcg cgcgcgcgcc gacgccgagc gctggatgga ctgggccttt
    22381 accttccaga acgcgatgcg caacgtcttc tggggcctca tccgcacgcc gcccgagaag
    22441 cgcgacgcca aggcgatcga ggacggcgcg aagaagagcc atgagctcgc cgccctgctc
    22501 gaccaggcgc tcgccggcaa accctatgtc gccggcgcgg ccttcaccat gggcgacatc
    22561 ccgatcggcg ccgaagtgca gcgctacatg cgcgtgccga tcgagcggcc tgcgctgccg
    22621 cacctgcacg cgtggttcga gcgcctgcgc gggcgtcccg cgttcctcaa gcacgtcgac
    22681 ctacccctga cgtgagcaag gtcgcgatcg tcaccggcgc ggcgagcggc atcggcctcg
    22741 cctgcgccga gcggctggcc gccgacggca tgacggtggt gctcgccgac ctgaacgaga
    22801 agaccggcgc ggaacatgcg aagcgcctcg gcggcagctt cgtcgccgcc gacctcggca
    22861 ggcgcgaggg ttgccgcaag ctgatcgacg cgaccctcaa ggcgcacggc acggtgcacg
    22921 tgctcgtcaa caacgccggc ttccagcacg tctcgccgat cgaggcgttc cccgaggacc
    22981 ggtgggagca gatgatcgcg ctgatgctca ccgcgccgtt cctcctcacg cgctattgct
    23041 ggccagcgat gaaaagacag aaatggggcc gcgtggtgaa catcgcctcg atccacgcgc
    23101 tcatcgcctc gcccttcaag gtcggctaca tcgccgcgaa gcacggcctc gtcggcctca
    23161 cgcgcaccgc ggcgctagaa ggcggcgagc acggcatcac cgtgaacgcc atctgcccgg
    23221 catacgtgag gaccccgctg gtggacagcc agatcgccga ccaggcaaaa gccaaccgca
    23281 tgtcgccgga ggaggtgatc gagaaaatca tgctcgcgcc ggcggcggtg aagcgcctga
    23341 tcgagcccgc cgaggtggct gacttcgtcg cctacctgtg cggcgacgcg gcgggcacca
    23401 tcaccggcgc cgcgttgacc atggacttgg gctggaccgc gcgctaacct agcagcggga
    23461 ggtttgcatg agcccgagtg caaaaccctg cagcacgtgc ggcaaggagg tggcccccgc
    23521 gacggtcgag acgatggccg gcgaggaagc aggcctgcgc atgagcatcg acggcatgcc
    23581 ggtgctgcgc tgcggcgagg ggcaccagcg cttcgtcacg ccggatttcg ccatccggct
    23641 catgcaggcg atcaccggcg acggccccat cgcgccgatc gacgcggcag tgcaaaaagg
    23701 gctgtttcgc aagcgcctgc actgcccggc ctgcggcaag gagcttggcg agcccgccgc
    23761 ggagcgcgcc cagatatcgc gcccgctgga gctcggcggc ggcgccgcat tcagcgtgcg
    23821 catcgagctg ccgaagtatc gctgcgccgc ctgcgccaag gaatgcctgc cgccggaggc
    23881 gatcctgggt gacgcgctga tgaaagcctc ggtgcacgcc tttcgttccg ccgcgttgcg
    23941 gcctacctga gctgcgcgtt gcgctcggcc ggcggcaggc gggcgagcga gcgcacccga
    24001 tcgtagtacc gcgccaggtc gccgccgctt gcggcgagca gcctgtcgaa tgccggcacc
    24061 aggtccgtgt aggttgcgta ggacgcgacg aaggcgttgt tcggctcctg cccctcgaaa
    24121 gcgggcatgc gcgcgaccag cggccgcaag gtttccagcg ccgcgcgctt tctctcccgc
    24181 atcgcctcgg gcgaaaggcg cgtgcggtac agcgcctcga gctgcgcgcg caccgcggcg
    24241 acctgcggcg cggggtcgaa tctcggcgcg ggctgtttcc cctgcgccgc cagccagcgg
    24301 cgcaggccct cgcgctccac caccaccgcg aaggactcgt tgaaggtgga atcgtccttc
    24361 acatacgcca cctggtgcgc gagctcgtgg aacatcagcc gcgccaggtc cgcttccggg
    24421 tagtggatga aggtggacag cagcgggtca tcgaaccagc cgagcgtcga ataggccgga
    24481 acgccgtaga cgacgacgtc gtggcccagc gcgcgctgcg ccgaggcatg ccgctcggcg
    24541 gcctccttcc cgtagtagcc gcggtagctc acacagccgg cgatcggaaa gcaggattgc
    24601 accggcgcga gcgagaactc gggcgccgcc accacgttcc acagcgcgta gggccgcccg
    24661 aggtcggcgt agctgcggta ggcgccgttg tcgggcagcg cgagctcgcg cgaggcgaaa
    24721 tcgcggatcg cgcgtgccag gccgagccgc tcctgcaacg ccggggccgc cacgacctcc
    24781 tccaggggcc gcgcgcgcgc catcagggcg agctggccgc ctgcggcctg gccgtagtac
    24841 gacagcgtct cgcagccgcc gagcaggact gcgctaagca gcagaaaagc gcgcatgcgc
    24901 gaggaaagac ccgacttgcg cggcgacgcg cgccgaaagc agcatcgcgc tatggcccac
    24961 cggcagaacc atgcgttggc gcattccttc gatcgccgtc tcctcgagac gcacgacgcc
    25021 gtcgttcggc ccggggagcg cgccgaacaa cctccccagg ccgaccggca gcgtccctgc
    25081 gaccacgccg agcggctcgg gccgcgtcca gcgcgccgcg cggccctcgc gccacagcgg
    25141 ctcgctcgcg cccaggaacc agcgccccca gcccaggcgc gcgaggcgtc gcccggcgag
    25201 gttgccgagc accggcgtgc cgagcagcac gacgtttccc gctgccgtgg cgggatggcg
    25261 cagcagggtt tcgaggacca ccagcccacc gaggctatgc ccgacgaagt gcgcgggtcc
    25321 gatgccgcgc gcaaagcgcg ccaggcgctc gccatgcgct tccagcggcc gctcgcgccc
    25381 ggcatagccg aacagatggg accgcaagcc ggcggattgc agccgcgccg cgagcggcgt
    25441 catcaccagc gcgggcaccc acagtccgtg caccaggacg gcgtcgcgca ccacgctcag
    25501 gcggcgctgc ggccggggag cgcgaagatg ccgctcatgc gcacgatgcg ggtctcgccc
    25561 acgcagaggt agccggtggc gaaggccagc cgcgagccca tgcgctgcac ctcgacttcg
    25621 gagtggagcc agtcgccgac cgaggccgcg ccggcgtagt cgatgtccag gtgcgcggtg
    25681 acgaaaccgc cgcgcccgcc ggtcttcgcc atcatgccgt agccgagcgc caggtcggcg
    25741 agcgtggcga gcaccccgcc gtggcagatg tcgcgcgaat tcgagtgctt cctgtccacc
    25801 cgcaggccga gtgacaccac gccctcgccg cgcttctcgt agagcggccc caccagctcg
    25861 ttgaacgggc tcgccgacgg cagcggccgg aatccctgcg gaatctgcgt catcgccgcg
    25921 cgctcagcgg tcccggccgc ggatctcgcg cagcgtctcg agcagcgcgc gcgcccggtg
    25981 cacgatgggg tagtccacca tctggccgtc cacctccacc gccgcagcgc ccttcgcctc
    26041 cgccgcctgg aatgcggcga cgatgcgctc ggcgcgcgcc gcctcctcgt cgctcggcgt
    26101 gaacgcggcg ttcgccggcg gcacctggtc cgggtgcacg cacaggcgac cctggaagcc
    26161 gcctcggcga ctgcgctcca gggcgcgctg gtagccctcc atgtcgcgca ggtggaacca
    26221 ggggctgtcg agcggccctt ccatgcccgc ggcgcgcgag gcgaggacca cgcgtgcgcg
    26281 cgcctcggcc agctcgggtt cctcgtggga aggaacgatc ccggtatcca ggctgaagtc
    26341 cgcggcgccg aacgccacgc gcttcacgcg ccaggggccc ggatagggcc gcaggttcct
    26401 cgcctgcacg atgcggtcca cgcgcgcgac gccggccgcc gtttcgatct ggggaatcag
    26461 gtcgatgctg ccctcggcga tgccctgctc gcgctccagg gcggcgagca gccagtcgat
    26521 ggcgtgcagg tcggcggcgc tctccacctt gggcagcacc acgccgtcca ccttgccgtg
    26581 gatcgtttcc accaggtcgc ggtagcaata cggcgacgac ggcgcgttcg ggcgcacgta
    26641 gccccggcat ttgcgcgggc gcttcagcgc ctcagcgacg ggcctgcgcg cggcggcctt
    26701 gtccgagacc gccaccgcgt cctccaggtc gaggattacc gcgtcggcgc cgagggacag
    26761 cgctttctcc acccgccgcg ggtgattgcc cggcgcgaac aggaacgacc tattgagcgg
    26821 catggacctg cctttcatcc cagcgtggat acggaagacg ccattgtgcc gtggcgagcg
    26881 cgcgcggcca gaccacctcg cgccggccgg cgaggatttg caccacggcc ggcctggcgg
    26941 ccacctgggc gccggtcttg tccacgcggt agcgcccgac cggcgtctcc gtgtcgagcg
    27001 ccgacagcgc ctcgcgcagc cgttcctggt cgagcgagcc ggacttgcgc accgcctcct
    27061 ccagcaccag ggcggcggcg taggcgccgg ccgcgagggc gccgggctcg ccctgccagc
    27121 gcttgcgcca tgcctcgacg aacgcggcgt tgccgcgcgt ggcaaaacgc gtgtcgtagg
    27181 cgacgaggcc gagggcgaac tcggcatcct gccccacgaa ctgcaggaaa cgcggctccg
    27241 ccgcgccctg cgcgaggaac acccagggcg catagccgat gcgcttgaac gacttcacca
    27301 tctccgcggc gtcctcgggc tggccgaaag ccacccagat ctcggcattg cgcgcgcggg
    27361 ccgcggccac ctgcgcggca tagtcgcgca ggccggcggc gaccgtctgc gccacgcccg
    27421 gctccagccc cagggcacgc gcctgctcga ccagccgcgc cgccgattcc cgcgaccccg
    27481 gatcgttgcg cgccaccacg tgcaggctgc ggtaacccat ggcgcgcgcg atggcgagca
    27541 cccccgcgcc gtaatcggcg agcggcgccg gcacctggaa cacgtagcgc gagccggcgc
    27601 ggtgcaggtc gcgcgaaatg cccgctgcat tgaccagcac ccggcgctgg cgctcggcat
    27661 acgcggcggc caccacgcag gcggcgttgc cgaaaggccc gatgagcagc tgggcgcctt
    27721 cggcatcgac cagccgcgcg tacagcctgc ccgcggcgct ggcctccgag tggtcgtcgg
    27781 cgatgcgcag gtccacgcgc cggccgagca ggccgcccgc ggcgttgcgc tgctcctgcc
    27841 acaggacgag cgccttcttc atgtcggcgg cgaggtcggc cagcagcccg gtctcgggca
    27901 ggaccacgcc cagcacgacc ggcggtccct gcgccaaggc cggggccgcc gcgaggctca
    27961 gcgcggcgag ccaggcgcgc atcagcagcc gaaaaaacgg gcgaagcgcg ccaccggctc
    28021 gcgcaccgtg accagcgtgt tctcgatggc gtcgggacgc gcgtagccga gcgacatgcc
    28081 gcagaccacc atctccggct cgggaatctg cagcacccgg cgcagcacgg tgtgcgccga
    28141 ggcgatcgcc gcctgcggac aggtatcgag gccgaagcca cgcgccgcca gcatcaggtt
    28201 ctgcaggaac atgcccgtgt ccagccaggc gcccaccggc aggtcgcgct ccagcgtcac
    28261 caccaggccc accggcgcgt cgaagaaaac gtagttgcgc gcgtgctggc ggtgggtctt
    28321 ctcgcgctcg cccttgccga tgccgagcag gccgtagagg tcccagccga gcttcctgcg
    28381 gcgcgcgaga tacgggtcgc gccacttggt ggggtagtac tcgtactcgc gcttccagcc
    28441 ctcctcgccg tgctgcatga actcggcgtg catcgcagcg gccagccgct cgcgcgcctc
    28501 gcccgccacc acgtagacgt tccagggctg gatgttggtg cccgacgggg cgcggctggc
    28561 gagcgccagg agccgctcga gcgttgcgcg cggcaccgga tcgggcttga aagcgcgcag
    28621 gctgcggcgc gaaaggatcg cttcctcgac cgagcgcgcg acgctcccgg ccgcgaaggg
    28681 cagcgcgccc agggcgggat catgagtcga cgggttcatc gggcgataat acccaacatg
    28741 gatgcccgcc tgcaggccgc gttcgctgcg ctggtcggcg tgaagcacgt cctcgccgcg
    28801 ccgcaggaca cttcgcccta caccaccgac tggcgccggc agtaccgcgg cgcggcgctc
    28861 ggcgtgctgc gcccggcgag caccgccgag gtcgccgcag tgctcggcct gtgcgcgcgc
    28921 gagcgcatcg ccgtggtgcc gcagggcggc aacaccggcc tgtcgggcgg atcggtaccg
    28981 acgggcaccc tgcccgaaat cgtattgtct ctttcgcgtc ttgcgcgcat ccgcgaggtc
    29041 gacacgctga acgacaccat caccgtcgag gcgggctgcg tgctcgccga catccagcgc
    29101 gcagcgcgcg aggccgggcg gctgtttccg ctgtcgctcg ccgccgaggg cagctgccag
    29161 atcggcggca acctgtccac gaacgccggc ggcgtgaatg tcctgcgcta cggcaacgcg
    29221 cgcgagcagg tgctgggcct ggaggtcgtg ctgccggatg gccgcatctg ggacgggctg
    29281 cgcgcgctgc gcaaggacaa caccggctac gacctgaagc agctgttcct cggcgccgag
    29341 ggcacgctgg gcgtgatcac ggcggcggtg ctccggctgc accccagtcc gacggcgagc
    29401 gccacggcgt ggatcgccgt gcagagcccg gcgcgcgcgg tcgagctgct ggccggcctg
    29461 cgccagcggc tcggcgagcg catcagcgcc ttcgagctgg tgtcgcgccg ctgcctcgag
    29521 gcggtgctgc agcatgcgag cgccgtgcgc gatcccttgc cgcgcgccta cccctggtac
    29581 gtgctcgccg agatcaccga ctcgggggac gatgcgagcc tggcggcgca ggtggagcag
    29641 gccttcggcg catgctcgga cctcgtggac gacgtggtcc tcgcctccag cgcctcacaa
    29701 tcgcaggcgc tgtggaagat ccgcgagacg attcccgagg cgcagttcgc caacgtcaag
    29761 cacgacgttt ccgtgccggt gtcgcgcgtg ccggatctga tcgagcgcgc gggcagggcg
    29821 ctcgaggcag cctttgccgg cgccccggtg tatgccttcg gccacatcgg cgacggcaac
    29881 atccactaca acgtcggcga ccaggcgctc cttgcccggc gcgccgaggt cagccgcgtg
    29941 gtgtatgacg cggtggcggc gctgggcggc tcgatatcgg ccgagcacgg cctcgggca
//
LOCUS       DASRIE010000219         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3894941, whole genome shotgun sequence.
ACCESSION   DASRIE010000219 DASRIE010000000
VERSION     DASRIE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3894941"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VFB53_09435"
     CDS             complement(<1..2022)
                     /locus_tag="VFB53_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN86991.1"
                     /translation="MKGIIALCALLASSCASAQTLTVGSKRFTESYVLGEIVSQTANG
                     THKPGLGNTAILLEALKAGAIDVYPEYTGTIAREILKSEDRLDLAALNAKLKPLGLAA
                     SIPLGFSNSYALGMRRKDAERLGIRKVSDLTKHPAQRFGLSHEFLGRRDGWPGLQGAY
                     GLPQKPRGLDHGIAYEALAAGEIDVMDLYTTDAKIERYAIVALEDDRRFFPPYDAVLL
                     YRADTPTRFPKEMQLLKVLEGRIDAATMVKLNARAEIDKVSFGDVAREYLGSGVQARK
                     GLWAALFAADFGRLLTEHLGLVFGSLAIAALIGIPLGMLAARVPWLAQPVLVSTGLLQ
                     TIPALALLALLIPVTGTIGVWPALIALFLYALLPITRNTHTGITEVRRGLVDAGTALG
                     LSRRRVLAHVELPLALPVIMAGVKTAAVISVGTATIAAFIGAGAPGSVGGAVRAGLRP
                     PAGRASWPGVRLARHRGADRHPPRHAGGARARARAAGADRHRPAANHPGARLARPPDP
                     GHRHHRRVAGADRAVPLRAAADHAQHPYRDHRSAARAGGRRHRAGAVAAARPGSCRAA
                     ARPAGDHGRGEDRGGNQRRHRDHRRLHRRRRLRRAHYPGPRAQRPHHAAGRRAAGRRA
                     RARGARRVRARRTRAIEEIRLSYHSAPCPSSSVYPARPSRVRSASPPSPR"
     gene            complement(1973..>2453)
                     /locus_tag="VFB53_09440"
     CDS             complement(1973..>2453)
                     /locus_tag="VFB53_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN86992.1"
                     /translation="RPQATREAVLAACEKACALEFIERLPQGLDSPLGERGIMLSGGQ
                     RQRLSIARALLADRPILLLDEATSSLDAAAERLVQQGLEALEQGRTTLAIAHRLATVQ
                     NADRIIVLERGAIVAQGTHAELMAQGGLYASLARLQFLGGRDEGDHRALRAVGQQLR"
BASE COUNT          389 a          837 c          879 g          348 t
ORIGIN      
        1 cctcggggac ggtggcgacg cgcttctcac ccgcgaaggt ctcgcggggt acaccgatga
       61 gctggggcat ggggccgaat gatacgacag ccttatctct tcgatagcgc gcgttcggcg
      121 agctcgaacg cggcgtgcac cacgagcgcg agcacggcgg ccggcagcgc gccggccagc
      181 agcatggtgt ggtcgttgag cgcgaggccc tgggtaatgc gctcgccgaa gccgccggcg
      241 ccgatgaagg cggcgatggt cgcggtgccg acgttgatta ccgccgcggt cttcaccccg
      301 gccatgatca ccggcagggc gagcggcagc tcgacatgaa ccaggacgcg ccgccgcgac
      361 agccccagcg cggtgccggc gtccaccagc ccgcgccgca cttcggtgat cccggtatgg
      421 gtgttgcgcg tgatcggcag cagcgcgtag aggaacagcg cgatcagcgc cggccacacg
      481 ccgatggtgc cggtgaccgg gatcaggagg gcgagcaagg cgagcgccgg gatggtttgc
      541 agcaggccgg tggcgatcag caccggctgc gcgagcccgg gcacgcgcgc cgccagcatg
      601 ccgaggggga tgccgatcag cgccgcgatg gcgagcgagc cgaacaccag gccaagatgc
      661 tcggccagca ggcggccgaa gtccggcgcg aacagcgccg cccacagacc ctggcgcgcc
      721 tgcgccgatg aaggcggcga tggtggcggt gccgacgctg atcaccgccg cggtcttcac
      781 cccggccatg atcacgggca gggcgagcgg cagctcgaca tgagccagga cgcgccgccg
      841 cgacagcccc agcgcagtgc cggcgtccac cagcccgcgc cgcacttcgg tgatcccggt
      901 atgcgtgttg cgcgtaatcg gcagcagcgc gtagaggaac agcgcgatca gcgccggcca
      961 cacgccgatc gtcccggtga ccgggatcag gagggcgagc aaggcgagcg ccgggatggt
     1021 ttgcagcagg ccagtgctga ccagcaccgg ctgcgcgagc cacggcacgc gcgccgccag
     1081 catcccgagc ggaatgccga tcagcgccgc gatggcgagc gagccgaaca ccaggccgag
     1141 atgctccgtt agcaggcggc cgaagtcggc tgcgaacagc gccgcccaga gacccttgcg
     1201 cgcctgcacc cccgatccga ggtattcccg cgccacgtcg ccgaagctca ccttgtcgat
     1261 ctcggcgcgc gcgttcagct tgaccatggt cgcggcatcg attcggcctt ccagaacctt
     1321 gagtaactgc atctcttttg gaaagcgcgt cggcgtgtcc gcccgataca ggagcaccgc
     1381 gtcgtagggc ggaaagaacc tccgatcgtc ctccagcgcg acgatcgcgt agcgctcgat
     1441 cttcgcatcg gtggtgtaca gatccatgac gtcgatttcg cccgcggcca gcgcctcgta
     1501 ggcgatgccg tggtccaggc cgcgcggctt ctgcggcagg ccgtatgccc cctgcaggcc
     1561 gggccagccg tcgcgccggc cgaggaattc gtgcgacagc ccgaagcgct gcgccggatg
     1621 cttcgtcagg tcggagacct tgcggatgcc caagcgctcg gcatccttgc gccgcatgcc
     1681 gagcgcgtag ctgttgctga aaccgagcgg aatggatgcc gcgaggccga gcggcttgag
     1741 cttcgcgttg agggcggcga ggtcaagccg gtcctccgac ttgaggattt ctcgcgcgat
     1801 ggtgccggtg tactccggat agacgtcgat cgcgcccgcc ttcagggcct cgagcaggat
     1861 cgcggtattg ccgagccccg gcttgtgcgt gccgttcgcc gtctggctga cgatctcgcc
     1921 cagcacgtaa gactcggtaa agcgcttcga gccgacggtg agcgtctgcg cgctagcgca
     1981 gctgctggcc aacagcgcgc agagcgcgat gatccccttc atcccttccc cccaagaact
     2041 gcaggcgcgc gaggctggcg tagagaccgc cctgcgccat caattcggcg tgggtgccct
     2101 gcgcgacgat cgcgccgcgc tcgagcacga tgatgcggtc agcattctgt accgtggcga
     2161 ggcgatgcgc gatcgcgagc gtggtgcggc cctgttcgag cgcttcgagg ccctgctgca
     2221 ccaggcgctc ggccgcggcg tcgagcgagc tggtggcctc gtcgagcagc aggatgggcc
     2281 ggtcggcgag cagcgcgcgg gcgatggaca ggcgctgcct ctggccgccc gagagcatga
     2341 tgccgcgctc gccgagcggc gaatccaggc cctggggcag gcgctcgatg aattcgagcg
     2401 cgcacgcctt ctcgcaggcg gcgagcaccg cttcgcgcgt cgcctgcggc cgg
//
LOCUS       DASRIE010000220        12379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2295712, whole genome shotgun sequence.
ACCESSION   DASRIE010000220 DASRIE010000000
VERSION     DASRIE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2295712"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1376
                     /locus_tag="VFB53_09445"
     CDS             177..1376
                     /locus_tag="VFB53_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZN86993.1"
                     /translation="MGRQNGVYQDAKGRWRVDKLHRGERLQGRFGSHQEAEAWLIAKC
                     AQIDAYGPVSARSMTLAEAATRYLLEEERAGKPWLSTESYLLEPVVECVGHLQLDQLH
                     DGTLRPFVDKRLAEGRSHKTVNLSLGVVRHMLNRACRKWRVDVGGGRTVPVLLQVPLL
                     TMLPLNDHQRKPQPISWDEQRRLMPLLPAHLARMSLFTLNTGARDNVVVSLRWAWEIQ
                     VDLGDIQCSVFEVPKEYVKGRKSEGYVVCNSVAQAIIEQVRKHHEEFVFVWRRERVKN
                     VDKEPAMKYQPTESMNNTAWQTARRKAGLGDLHVHDLRHTVGMRLREAGVKEDTRADI
                     LWHVRPGMTAHYSEAQIREIRAALELIKDETGRQNRSLRSLAKEAREGHAAAVSPFGV
                     PSPRKTA"
     gene            complement(1416..1492)
                     /locus_tag="VFB53_09450"
     tRNA            complement(1416..1492)
                     /locus_tag="VFB53_09450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Arg,seq:acg)
     gene            complement(1527..2774)
                     /locus_tag="VFB53_09455"
     CDS             complement(1527..2774)
                     /locus_tag="VFB53_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HZN86994.1"
                     /translation="MRAVVWFLLLFAVAVVAATTLGTNDGIVSVYWAGWRLDTSLNLF
                     LIALVGTCFVLVSVIQAVSSLTRLPQRAHEWRVARRDRSAQAALRDALAQYFGGRYSR
                     AQTAAQKALAIQADTPDLAQDNEFTVLGHLLAAGSAHRLQDRAKRDEQLKRALELART
                     SSAARSAEEGARLLAAEWALDDRDAARALELLGELPPGVARRTHALRLKLQATRLGRQ
                     PQEALKTARLLAKHQGFSKVAAQGLLRSLAAESLATAHDIDQLRRVWQQFDNADRRDP
                     VVAAGAACRAAELGAPEDARAWLRPFWERLDELTEDERATVAEALVAALPGIGPEWLA
                     RLEAASRAFPRDAAIAHAVGSALAERRLWGKARQLLEVAAEDAGLALDQRRRAWLTMG
                     RMAEEEGQNERAVQCFEAAARLG"
     gene            complement(2785..3846)
                     /locus_tag="VFB53_09460"
     CDS             complement(2785..3846)
                     /locus_tag="VFB53_09460"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZN86995.1"
                     /translation="MRRETSPVPASTPSRRWAAAVAVVLLALSLAALALGWSAERRVK
                     TLQEELVRRQQDSATQAAEARLLARQAQEGMRDNAARISLMESRMGEVSLQRGQLEEL
                     IQSLSRSRDENVLVDIEAGVRVAVQQAAITGTAEPLIAALKQGDERLARFNQPRLEGV
                     RRAIARDLDRVKAVGTADLPSLSIKLDEAVRLVDELPLLSVAEPRRTAPTATPAAAAS
                     TGAPAPAGNWARVWQDRWDGATGRVWSEVKSLVRVTRIDRPEGMLLAPDQAFLVRETL
                     KLRLLNARLALLARQFDTAQSDLRAAQDALDRYFDKSARRHALAADLIRQVGAQARQV
                     SLPRPDDTLAALAALSAGR"
     gene            complement(3891..4658)
                     /locus_tag="VFB53_09465"
     CDS             complement(3891..4658)
                     /locus_tag="VFB53_09465"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN86996.1"
                     /translation="MRVLVTRPAGQADTWVRALAARGVDAVALPLIDIAAPHDPAPVQ
                     AAWRALGDSRLAVFVSPNAARAFFQARPQGAAWPASTRAASVGPGTTQALRALGVPPS
                     SIVEPHEDSAQFDSEALWQRLERDDWRGAHVLVVRGDGGRDWLADTLRGAGAEVAFVA
                     AYRRAAPRFDGECGARLAYALAHPESHVWLFSSSEAIDHLMHAPQPAAGADWSRARAI
                     ATHPRIASRARERGFGAVLECRPTVDAVVACIQSRAS"
     gene            complement(4670..5596)
                     /gene="hemC"
                     /locus_tag="VFB53_09470"
     CDS             complement(4670..5596)
                     /gene="hemC"
                     /locus_tag="VFB53_09470"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN86997.1"
                     /translation="MAQRIIIATRESRLALWQAHHVQDLLAQRFGAQVDLLGMTTQGD
                     QILDRALSKVGGKGLFVKELETALEEGRADLAVHSLKDVPMDLPPGFALAAVLEREDP
                     RDAFVSPKYASLEALPQGARVGTSSLRRVVQLRALRPDLVIEPLRGNLDTRLRKLDEG
                     QYDAIVLAAAGLKRLGLAGRIRGVFEPEQMLPCAGQGALGLETRADAAPLLAQLDTLR
                     HAPTQLAVEAERAVSRALGGSCSMPLAAFARWQGDTLDLRVALGHPERTDAALLRSQA
                     QARVTTAEEARALGDRAAQALRDQGAAAYLAS"
     gene            5717..8491
                     /gene="ppc"
                     /locus_tag="VFB53_09475"
     CDS             5717..8491
                     /gene="ppc"
                     /locus_tag="VFB53_09475"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZN86998.1"
                     /translation="MARTADDAAEKNRPLVEDIRLLGRLLGDVIREQEGKAAFELIER
                     IRQLSVAYRLKRDAQAGRALDRLLKNLSGDQTVSVIRAFSYFSHLANIAEDRHHVRRR
                     DVHERQGHLQEGSLAMSLERLHDAGIRATDIARTLAHAYISPVLTAHPTEVQRKSILD
                     AERAIAQLVEARETLPTARDLADNEALIRARITQLWQTRMLRTAKLTVADEIENALSY
                     YRTTFLRQIPRLYREIETALPGHPIASFFRMGNWIGGDRDGNPFVTADTLRMALARQS
                     ETVLRFYLTEVHELGAELSISNMLAPVTPEMRALADASPDHSDHREDEPYRRALIGMY
                     ARLAATLHELTGTEALRHAVAPQNPYREASQLLAELRVIEASLVSHHAEALIEPRLRP
                     LMRAVQVFGFHLATVDLRQSSDQHETVIAELLQVARIEPDYRALGEPARRALLLKLLD
                     DARPLRVRAAAYSEHTRNELAIFEAALEMRERYGSEALRHYIISHTEQASDLLEVLVL
                     LKEAGLLRGTLDDRAQADLIVVPLFETIADLRHAEPIMREYYALPGLAAMMKRSGGEQ
                     DVMLGYSDSNKDGGTFTSNWELYCAETALVELFAALGREHGLTLRLFHGRGGTVGRGG
                     GPSFQAILAQPPGTVNGQIRLTEQGEVIASKYAHPEIGLRNLETLVAATLEATLLHPT
                     QSAPPAFLQAAGALSEASFAAYRKLVYETPGFTDYFFSATPIREIAELNIGSRPASRK
                     PTRAIEDLRAIPWSFSWGQCRVALPGWAGFGSAIEAWLDDAATRKSRLALLRRMHREW
                     PFFRALLSNLDMVIAKSDLGIAARYVELVEDKRLGKRIFGLIEAEWRRTDEALTLITG
                     EKRRLASNPALARSIEHRFPYLDPLNHLQVELMRRYRQRRDGDPGLERVQRGIHISIN
                     GVAAGLRNTG"
     gene            complement(8516..9199)
                     /locus_tag="VFB53_09480"
     CDS             complement(8516..9199)
                     /locus_tag="VFB53_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HZN86999.1"
                     /translation="MQRRGFIAGALAFGGLLGGTAPARALSESEAAGGIRAALERGAV
                     SAVALLGRTDGFLGNPKVRIPLPGFLEDAARLLRTMGQQRRVEELETAMNRAAEAAVP
                     EAKSLLVGAVKSMSVDDARRILGGGDDAATRFFAEKTREPLAGRFLPIVTRATRKVAL
                     AEKYNAIAGRASALGLLRQEDANLQQYVTGKALDGLYLMIGEEERRIRQDPIGTGSAL
                     LKRVFGAPR"
     gene            complement(9210..9980)
                     /locus_tag="VFB53_09485"
     CDS             complement(9210..9980)
                     /locus_tag="VFB53_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN87000.1"
                     /translation="MEVLRVLLVDDEELARVRLRSLVDECPQPKCRVAGEAANATQAL
                     VWLQAQACDVILLDVRMPGRDGMQLAAELRQRLQPPAVVFVTAHAEHAVRAFDLEAVD
                     YLTKPVRRERLHAALQRVAARLALPAAAAAAPSESEPVVVVSDRGRVVRVPVSEVLYL
                     KAELKYVTLRTAEHTYVLDDALADLEQRLGDRFIRVHRNALVARGAVRALERRAIAGS
                     EDEGGESWAVRVAMVDEWLAVSRRQATAVREALVGAGL"
     gene            complement(9996..11135)
                     /locus_tag="VFB53_09490"
     CDS             complement(9996..11135)
                     /locus_tag="VFB53_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN87001.1"
                     /translation="MSPPPTPREPDTGFATTQAPTGWGHSTRPLDTRGTAFGSTLLDL
                     PLQAPGGAGAPQAFDACHVGVVLRAVVFVNGVVAVGVMFAARDLASWAALVTVGASVA
                     LPGVLLWLMAACLLKRWLSALPVAAQWAAALALGAASGGFGWAIGGSPGLVAGDLGHA
                     PAALLAGAGLAAAVFQWLRMRAKAKLPADTTARLAELQSRIRPHFLFNTLNTALSLVT
                     VDPARAEIVLEDLAELFRVALTDSGESVSLGEEVELAQRYLAIEQMRFGDRLRVQWEL
                     DADAGRARVPPLLLQPLVENAVRHGVEPAPDGGVVRVRTRVRMGRVLVSITNTVPDTP
                     SRPGNGIALENVRERLRLMHDVAAQLDTRLERDVFRVQLVVPLER"
     gene            11216..>12379
                     /gene="argH"
                     /locus_tag="VFB53_09495"
     CDS             11216..>12379
                     /gene="argH"
                     /locus_tag="VFB53_09495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZN87002.1"
                     /translation="MSSNQLDKKSQAWSALFSEPMSELVKRYTASVSFDRRLWKADIA
                     GSLAHAEMLAAQGIVAPEDRAAIERGMAQIREEIESGRFEWKLDLEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRDEIDGLAALLRDMQRALVEVAARNVETILP
                     GFTHMQVAQPVSFAHHLLAYVEMFARDEERLADVRRRVNRLPLGAAALAGTSYPLDRE
                     RVARTLGFDGVCQNSLDAVSDRDFALEFASFAAIAMVHVSRLAEEIVLWMSQNFGFID
                     LADRYCTGSSIMPQKRNPDVAELARGKSGRVVGHLMGLITLMKGQPLAYNKDNQEDKE
                     PLFDTVDTLRDTLRIMAEMVGGITVKAEAMERAALKGYATATDLADYLVKKGLP"
BASE COUNT         1823 a         4554 c         4396 g         1606 t
ORIGIN      
        1 ctcttcgacc tgttcaagca gggcgcagac ccacgtcctt caacggtggc ggactgcgtc
       61 gagccgaact tcggtgacgt ggagcacccc accacgacct ccgccgaccc ccagccagga
      121 gccacggtat cctccctggt ggacaggcgg cccgcgtcga ggaaaggagt tgaaagatgg
      181 gtcgtcaaaa cggcgtctac caagacgcaa agggcaggtg gcgagtcgac aagctccacc
      241 gcggtgaacg ccttcaaggc cgtttcggat cgcatcagga agccgaagcc tggctgatcg
      301 ccaagtgtgc gcagattgat gcctacggac cggtcagtgc caggtcgatg accttggccg
      361 aggcggccac tcgatacctc ctggaggagg agcgggccgg caagccatgg ctttcgacag
      421 agagctacct gctggaaccg gtggtcgagt gcgtcggcca tctgcagctc gaccagctcc
      481 acgacgggac gctgaggccc ttcgtggaca agcggctggc agagggtagg tcgcacaaga
      541 ccgtcaatct gagcctcggg gtggtcagac acatgctcaa ccgcgcttgc cgcaagtggc
      601 gcgtggatgt cggaggcggt cggacggtgc cggtgttgct gcaggttccc ctgctgacga
      661 tgctgccgct gaacgatcac caacgaaaac cgcagccgat ctcgtgggac gagcagcgca
      721 gattgatgcc gttgctgccg gcacacctcg cccgcatgtc gttgttcacc ttgaacaccg
      781 gcgcacggga caacgtagtt gtgagccttc gctgggcctg ggagatccag gtcgaccttg
      841 gcgacatcca atgctcggtg ttcgaggtgc ccaaggaata cgtcaagggc cgcaagtcgg
      901 aaggctacgt ggtttgcaac tcggtggccc aagccatcat cgagcaggtc cgcaaacacc
      961 acgaagagtt cgtgttcgtt tggcggcgcg agagggtgaa gaacgtggac aaggaaccgg
     1021 ccatgaagta ccagccgacc gagtcgatga acaacaccgc ttggcagacg gctcgccgca
     1081 aggctgggct tggtgatctg cacgtccacg acttgcgaca cacggtcggc atgcgattac
     1141 gcgaagccgg tgtcaaagag gacacgcggg cggacattct gtggcacgtg cggcccggaa
     1201 tgacggcgca ctactcggag gcgcaaattc gcgaaatccg ggctgctctc gagctgatca
     1261 aggatgaaac cggtcggcag aaccggtcgc tcagaagctt ggcgaaggaa gcacgcgagg
     1321 gccatgcagc cgccgtgagt cccttcgggg tcccttcacc aagaaaaacg gcttaggcgc
     1381 tgtgacacct aagccgttga tcgaggagag aaatttggcg cggctggcag gattcgaacc
     1441 cacgacccct tggttcgtag ccaagtactc tatccaactg agctacagcc gcgcgagccc
     1501 tctagtatag cagcgttttt ttccggctag ccgagtcggg ctgccgcttc gaagcactgg
     1561 accgcgcgtt cgttctggcc ttcttcctcg gccatgcggc ccatcgtgag ccaggcgcgg
     1621 cggcgctggt cgagggcgag gccggcgtct tcggccgcca cctcgagcag ctgccgcgcc
     1681 ttgccccaga ggcggcgctc ggcgagcgcg ctgcccacgg cgtgcgcgat ggccgcgtcg
     1741 cgcggaaagg cgcgcgatgc ggcttcgagg cgcgcgagcc attccgggcc gatccccggc
     1801 aacgccgcga cgagcgcttc ggccacggtg gcgcgctcgt cctcggtgag ctcgtcgagc
     1861 cgctcccaga acggtcgcag ccacgcgcgc gcgtcctccg gcgcgccgag ctcggccgcg
     1921 cggcacgccg cacctgcggc caccaccgga tcgcggcggt cggcgttgtc gaactgctgc
     1981 cacacgcggc gcagctggtc gatgtcgtgc gcggtcgcga gcgactcggc ggcgagcgag
     2041 cgcagcaggc cttgcgccgc gaccttggag aagccctggt gcttggcgag caggcgcgcc
     2101 gtcttcagcg cctcctgcgg ctggcgcccc aggcgcgtgg cctgcagctt caggcgcagc
     2161 gcatgcgtgc gccgcgcgac gccgggcggc agctcgccga gcagctcgag cgcgcgcgcc
     2221 gcgtcgcggt cgtcgagcgc ccattcggcg gcgagcagcc gcgcgccctc ctccgccgag
     2281 cgcgcggccg agctcgtgcg cgcgagctcc agcgcgcgct tgagctgctc gtcgcgcttg
     2341 gcgcggtcct gcaggcggtg cgcgctgccc gcggcgagca ggtggccgag cacggtgaac
     2401 tcgttgtcct gcgcgaggtc cggggtgtcg gcctggatgg cgagcgcctt ctgcgcggcc
     2461 gtctgcgcgc ggctgtagcg cccgccgaag tactgcgcga gcgcgtcgcg cagcgcggcc
     2521 tgcgcgctgc ggtcgcgccg cgccacgcgc cactcgtgcg cgcgctgcgg cagccgcgtg
     2581 agcgagctca ccgcctggat caccgacacc agcacgaagc aggtgccgac gagcgcgatc
     2641 aggaacaggt tgagcgacgt gtcgagccgc cagccggccc agtagacgct cacgatgccg
     2701 tcgttggtgc cgagcgtcgt ggcggccacg acggcgacgg cgaacagcag caggaaccag
     2761 acgacggcgc gcatccgtgc tcgctcagcg ccccgccgaa agggccgcga gcgcggccag
     2821 cgtgtcgtcc ggccgcggca ggctcacttg ccgcgcctgc gcgcccacct ggcggatcag
     2881 gtcggccgcc agcgcgtggc ggcgcgcgga cttgtcgaag tagcggtcga gcgcgtcctg
     2941 cgcggcgcgc aggtcggact gcgccgtgtc gaactgccgc gccagcagcg ccagccgcgc
     3001 attgagcagc cgcagcttca gcgtctcgcg caccaggaac gcctggtccg gcgcgagcag
     3061 catgccttcg gggcgatcga tgcgcgtcac gcggaccagc gacttcacct cggaccacac
     3121 gcggccggtg gcgccgtccc atcggtcctg ccacacgcgg gcccaattcc ccgcgggcgc
     3181 gggcgcgccg gtcgacgccg cggccgccgg cgttgcggtg ggcgcggtcc ggcgcggctc
     3241 ggccaccgag agcagcggca gctcgtcgac gaggcgcacc gcctcatcga gcttgatgga
     3301 cagcgacggc aggtccgccg tgccgaccgc cttcacgcgg tccaggtcgc gcgcgatggc
     3361 gcggcgcacg ccttcgagcc gcggctggtt gaagcgcgcg agccgctcgt cgccctgctt
     3421 cagcgccgcg atgagcggct cggccgtgcc ggtgatggcg gcctgctgca ccgccacgcg
     3481 aacgcccgct tcgatgtcga ccagcacgtt ctcgtcgcgc gagcgcgaca gcgactggat
     3541 cagctcctcg agctgcccgc gctgcagcga cacctcgccc atgcgcgact ccatgaggga
     3601 gatgcgcgcc gcgttgtcgc gcatgccttc ctgggcctgc cgggccagca gccgggcctc
     3661 ggcggcctgc gtcgcgctgt cctgctggcg gcgcaccagc tcttcctgca gcgtcttcac
     3721 ccgccgctcc gcgctccagc cgagggcgag cgccgcgagg gagagcgcga gcagcacgac
     3781 ggcgaccgcc gcggcccagc ggcgcgacgg cgtggacgcc ggcacgggcg acgtctcgcg
     3841 gcgcacgggg gacgggggcg gcggcgggag gaggtcgacc gggggttcgc tcacgatgcg
     3901 cgcgattgta tgcaggccac cacggcgtcg acggtgggcc ggcactcgag caccgctccg
     3961 aagccgcgct cgcgcgcacg ggaggcgatg cggggatggg tcgcgatggc gcgcgcgcgc
     4021 gaccaatccg cgcccgccgc gggctgcggc gcgtgcatca ggtgatcgat cgcctccgag
     4081 ctgctgaaga gccacacgtg cgactcggga tgcgccagcg cgtaggcgag ccgcgccccg
     4141 cactcgccgt cgaagcgcgg cgcggcgcgc cggtacgcgg cgacgaacgc cacctcggcg
     4201 ccggcccccc gcagcgtgtc cgccagccag tcgcgcccgc cgtccccgcg cacgacgagc
     4261 acgtgcgcgc cgcgccagtc gtcgcgctcg agccgctgcc acagcgcctc ggaatcgaac
     4321 tgcgcgctgt cctcgtgcgg ctcgacgatg gacgatggcg gcacgccgag cgcgcgcagc
     4381 gcctgggtcg tgcccggtcc gacggaggcc gcccgggtgg acgccggcca ggccgcgcct
     4441 tgcggccggg cctggaagaa tgcccgcgcc gcattcgggc tcacgaagac ggcgaggcgc
     4501 gaatcgccga gcgcgcgcca cgccgcctgc accggcgccg ggtcgtgcgg cgccgcgatg
     4561 tcgatgagcg ggagcgcgac cgcgtcgact ccccgcgccg ccagcgcccg cacccacgtg
     4621 tcggcctgcc cggccgggcg ggtgacgagg acgcgcatcg ggaatccgat cagctcgcca
     4681 ggtaggcggc cgcgccctgg tcgcgcagtg cctgcgccgc gcggtcgccc agcgcgcgcg
     4741 cctcctcggc cgtcgtcacg cgggcctgcg cctgcgagcg cagcagcgcc gcgtcggtgc
     4801 gctcgggatg gcccagcgcg acgcgcaggt cgagcgtgtc cccctgccag cgcgcgaagg
     4861 cggccagcgg catgctgcag ctgcccccga gcgcacgcga caccgcgcgc tccgcctcga
     4921 ccgcgagctg cgtgggcgcg tgcctgagcg tgtcgagctg cgcgagcagc ggcgccgcat
     4981 cggcgcgcgt ttccaggccc agcgcgccct ggccggcgca cggcagcatc tgctcgggct
     5041 cgaacacgcc gcggatgcgg cccgcgagcc ccaggcgctt caggccggcg gccgccagca
     5101 cgatggcgtc gtactgcccc tcgtcgagct tgcgcaggcg cgtgtcgagg ttgccgcgca
     5161 gcggctcgat gaccaggtcc ggccgcaggg cgcgcagctg caccacgcgg cgcaggctcg
     5221 acgtgcccac gcgcgcgccc tgcggcagcg cctcgagcga ggcgtacttc ggcgagacga
     5281 aggcgtcgcg cgggtcctcg cgctccagca ccgcggcgag cgcgaagccg ggcggcaggt
     5341 ccatgggcac gtccttcagc gaatgcacgg ccaggtcggc gcggccctcc tccagcgcgg
     5401 tctcgagctc cttcacgaac aggcccttgc cgcccacctt cgacagcgcg cggtccagga
     5461 tctggtcccc ctgcgtcgtc atgccgagca ggtccacctg cgcgccgaag cgttgcgcga
     5521 gcaggtcctg cacgtggtgc gcctgccaga gtgcgaggcg gctttcgcgc gtcgcgatga
     5581 tgattcgttg ggccatgcgg gatgctagca gcgccccggg gcggcgtgcg cgcctgcgag
     5641 acaatcgcgg gccaggtaac tgacgcagcg caaggcacta gaatcacgta actttattac
     5701 acctggacga cctgcgatgg cccgaaccgc tgacgacgcc gccgagaaga atcgccccct
     5761 ggtcgaggac atccgcctgc tcggccggct gctcggcgac gtgatccgcg agcaggaggg
     5821 caaggcggcc ttcgagctga tcgagcgcat ccgccagctc tccgtggcct accggctcaa
     5881 gcgcgacgcg caggccggcc gcgcgctcga ccggctgctg aagaacctgt cgggcgacca
     5941 gacggtgagc gtgatccgcg ccttcagcta cttctcccac ctggccaaca tcgccgagga
     6001 ccggcaccac gtgcgccggc gcgacgtcca cgagcgccag ggccacctgc aggaaggctc
     6061 gctcgcgatg tcgctcgagc ggctgcacga cgcgggcatc cgcgccaccg acatcgcgcg
     6121 cacgctggcg cacgcctaca tctcgcccgt gctcaccgcg cacccgaccg aggtgcagcg
     6181 caagagcatc ctcgacgccg agcgcgccat cgcccagctg gtcgaggcgc gcgagacgct
     6241 gcccaccgcg cgcgacctgg cggacaacga ggcactgatc cgcgcgcgca tcacgcagct
     6301 gtggcagacg cgcatgctgc gcaccgccaa gctgacggtg gccgacgaga tcgagaacgc
     6361 gctgtcgtac taccggacca ccttcctgcg gcagatcccg cgcctgtacc gcgagatcga
     6421 gaccgccctg cccggccacc ccatcgcgag cttcttccgc atgggcaact ggatcggcgg
     6481 cgaccgcgac ggcaacccct tcgtcaccgc cgacacgctg cgcatggcgc tcgcgcggca
     6541 gagcgagacg gtgctgcgct tctacctcac cgaggtgcac gagctgggcg ccgagctgtc
     6601 gatctccaac atgctcgcgc cggtgacgcc cgagatgcgg gcgctggccg acgcctcgcc
     6661 cgaccacagc gaccaccgcg aggacgagcc gtaccggcgc gcgctcatcg gcatgtacgc
     6721 gcgcctggcc gccacgctgc acgagctgac gggcaccgag gcgctgcgcc atgccgtggc
     6781 gccgcagaac ccgtaccgcg aggcgtcgca gttgctggcc gagctgcgcg tgatcgaggc
     6841 ctcgctcgtc tcgcaccacg ccgaggcgct gatcgagccg cggctgcggc cgctgatgcg
     6901 cgcggtgcag gtgttcggct tccacctcgc cacggtggac ctgcggcaga gctcggacca
     6961 gcacgagacg gtgatcgccg agctgctgca ggtggcgcgc atcgagcccg actaccgcgc
     7021 gctcggcgag cccgcgcgcc gcgcgctgct gctgaagctg ctcgacgacg cccgcccgct
     7081 gcgcgtgcgc gccgccgcct acagcgagca cacgcgcaac gagctggcga tcttcgaggc
     7141 cgcgctcgag atgcgcgagc gctacggcag cgaggcgctg cgccactaca tcatctccca
     7201 caccgagcag gcgagcgacc tgctcgaggt gctggtgctg ctgaaggagg cgggcctgct
     7261 gcgcggcacg ctggacgacc gcgcccaggc cgacctgatc gtggtgccgc tgttcgagac
     7321 catcgccgac ctgcgacacg ccgagcccat catgcgcgag tactacgcgc tgccgggcct
     7381 ggccgcgatg atgaagcgct ccggcggcga gcaggacgtc atgctgggct acagcgacag
     7441 caacaaggac ggcggcacct tcaccagcaa ctgggagctg tactgcgccg agaccgcgct
     7501 ggtggagctg ttcgccgcgc tggggcgcga gcacggcctc acgctgcgcc tgttccacgg
     7561 ccgcggcggc accgtgggcc gcggcggcgg ccccagcttc caggccatcc tcgcgcagcc
     7621 gcccggcacg gtgaacgggc agatccgcct caccgagcag ggcgaggtca tcgcctccaa
     7681 gtacgcgcat ccggagatcg ggctgcgcaa cctcgagacg ctggtggccg ccacgctgga
     7741 ggccacgctg ctgcacccca cccagagcgc gccgcccgcg ttcctgcagg ccgccggcgc
     7801 gctgagcgag gcgagcttcg ccgcctaccg caagctggtg tacgagacgc cgggcttcac
     7861 cgactacttc ttcagcgcca cgccgatccg cgagatcgcc gagctcaaca tcggctcgcg
     7921 ccccgcctcg cgcaagccca cgcgcgcgat cgaggacctg cgcgccatcc cgtggagctt
     7981 cagctggggc cagtgccgcg tcgcgctgcc gggctgggcc ggcttcggct cggcgatcga
     8041 ggcctggctg gacgacgcgg ccacgcgcaa gtcgcgcctc gcgctgctgc gccgcatgca
     8101 ccgcgaatgg cccttcttcc gcgcgctgct gtcgaacctc gacatggtga tcgccaagag
     8161 cgacctgggc atcgcggcgc gctacgtcga gctggtggag gacaagcgcc tgggcaagcg
     8221 catcttcggg ctcatcgagg ccgaatggcg gcgcaccgac gaggcgctca cgctcatcac
     8281 gggcgagaag cggcggctgg cgtcgaaccc ggcgctcgcg cgctcgatcg agcaccgctt
     8341 cccgtacctg gatcccctca accacctgca ggtggagctg atgcgccgct atcgccagcg
     8401 gcgcgatggc gaccccggcc tcgagcgcgt gcagcggggc atccacatct cgatcaacgg
     8461 cgtggcggcg gggttgcgca acacggggtg agcggcgccc cggggcggcg agcggctacc
     8521 tcggcgcgcc gaacacgcgc ttcagcaagg cgctgcccgt gccgatcggg tcctggcgga
     8581 tgcgccgttc ctcctcgccg atcatgaggt agaggccgtc cagcgccttg ccggtcacgt
     8641 actgctgcag gttcgcgtct tcctgtcgca gcaggccgag ggccgacgcg cggccggcga
     8701 tggcgttgta cttctcggcg agcgccacct tgcgcgtcgc gcgcgtgacg atcggcagga
     8761 agcgcccggc cagcggctcg cgcgtcttct cggcgaagaa gcgcgtggcc gcgtcgtcgc
     8821 cgccgccgag gatgcggcgc gcgtcgtcca cgctcatcga cttcaccgcg cccacgagca
     8881 acgacttcgc ctcgggcacc gccgcttcgg cggcgcggtt catcgcggtc tcgagctcct
     8941 cgacgcgccg ctgctggccc atggtgcgca gcaggcgcgc cgcatcctcg aggaagccgg
     9001 gcagcgggat gcgcaccttc ggattgccga ggaaaccgtc ggtgcggccg agcagcgcga
     9061 cggccgacac cgcgccgcgc tcgagcgcgg cgcggatgcc gccggcggcc tcggactcgc
     9121 tcagcgcacg cgccggcgcg gtccccccca agagcccgcc gaacgcgagc gcgcccgcga
     9181 tgaatccccg tcgttgcatt cgatctcctt cagaggccgg cgccgacgag cgcctcgcgc
     9241 accgccgtcg cctggcggcg cgacaccgcg agccattcgt ccaccatcgc cacgcgcacg
     9301 gcccagctct cgccgccctc gtcctcgctg cccgcgatgg cgcgccgctc gagcgcgcgc
     9361 accgcgccgc gcgcgaccag cgcattgcgg tgcacgcgga tgaagcggtc gcccaggcgc
     9421 tgctccaggt cggcgagcgc atcgtccagc acgtacgtgt gctcggccgt gcgcaaggtg
     9481 acgtacttca gctcggcctt gaggtagagc acctccgaca ccggcacgcg cacgacgcgg
     9541 ccgcggtcgc tcaccacgac caccggctcg gactcggacg gcgcggccgc cgcggccgcc
     9601 ggcagggcga gccgcgcggc cacccgctgc agcgccgcgt gcaggcgctc gcgccgcacg
     9661 ggcttggtca ggtagtccac cgcctcgagg tcgaaggcgc gcaccgcgtg ctcggcatgc
     9721 gcggtgacga acacgaccgc cggcggctgc agccgctggc gcagctcggc ggcgagctgc
     9781 atgccgtcgc gcccgggcat gcgcacgtcg agcaggatca cgtcgcaggc ctgcgcctgc
     9841 agccacacca gcgcctgcgt cgcgttggcc gcctcgccgg ccacgcggca cttcggctgc
     9901 gggcattcgt ccaccaggct tcgcagacgc acgcgggcga gctcttcgtc gtcgacgagc
     9961 agaacgcgaa gcacttccat ggcgtactcc tcggatcagc gttcgagcgg cacgacgagc
    10021 tggacgcgga acacgtcgcg ctccagccgc gtgtcgagct gcgccgcgac atcgtgcatc
    10081 agccgcaggc gctcgcgcac attctccagc gcaatcccgt tgccggggcg cgacggcgtg
    10141 tccggcacgg tgttggtgat cgacaccagc acgcgcccca tgcgcacgcg cgtgcgcacg
    10201 cggaccacgc cgccgtcggg cgccggctcg acgccgtgcc gcaccgcgtt ctccaccagc
    10261 ggctgcagca gcagcggcgg cacgcgggcg cggccggcgt ccgcatccag ctcccactgc
    10321 acgcgcaggc ggtcgccgaa gcgcatctgc tcgatcgcga ggtagcgctg cgcgagctcg
    10381 acttcctcgc cgagcgacac cgactcgccg ctgtcggtga gcgccacgcg gaagagctcc
    10441 gccaggtcct cgagcacgat ctcggcgcgc gccggatcca ccgtcacgag gctcagcgcc
    10501 gtgttgagcg tgttgaacag gaagtgcggc cggatgcgcg actgcagctc ggccagccgc
    10561 gccgtcgtgt cggccggcag cttggccttg gcgcgcatgc gcagccactg gaagacggcc
    10621 gccgccaggc cggcgccggc cagcagcgcc gccggcgcat ggcccaggtc gcccgcgacg
    10681 agtccgggcg agccgccgat ggcccagccg aagccgccgc tcgcggcgcc gagcgccagc
    10741 gccgccgccc attgcgcggc cacgggcagc gcgctcagcc agcgcttgag caggcatgcg
    10801 gccatgagcc acagcagcac gccgggcagc gcgacggagg cgcccacggt gaccagcgcg
    10861 gcccacgacg ccaggtcgcg cgccgcgaac atcacgccca ccgcgaccac gccgttgacg
    10921 aacaccaccg cgcgcagcac cacgcccacg tggcacgcgt cgaacgcctg cggcgcgccg
    10981 gcgcctccgg gcgcctgcag cgggaggtcg agcagcgtgc tgccgaaggc ggtgccgcgc
    11041 gtgtccagcg ggcgcgtgga gtgaccccag ccggtgggcg cctgggtggt cgcgaagccg
    11101 gtgtcgggct cgcggggcgt gggagggggc gacatcgagg gggctcgata gaatcgagag
    11161 cttcgcattg tcgcgccaac gcccggcgct gccacgcccg gacgggccgc ttcgcatgtc
    11221 ctccaaccaa ctcgacaaga aatcacaggc ctggtccgcg ctcttctccg agccgatgag
    11281 cgagctcgtc aagcgctaca cggccagcgt gtcgttcgac cggcggctct ggaaggcgga
    11341 catcgcgggc tcgctggccc acgccgagat gctggcggcg caggggatcg tcgcgcccga
    11401 ggaccgcgcg gccatcgagc gcggcatggc gcagatccgc gaggagatcg agtcgggccg
    11461 tttcgaatgg aagctcgacc tcgaggacgt gcacctcaac atcgaggcgc gcctcacgca
    11521 gctcgtgggc gacgccggca agcggctgca caccgggcgc tcgcgcaacg accaggtggc
    11581 caccgacgtg cgcctgtggc tgcgcgacga gatcgacggc ctggccgcgc tgctgcgcga
    11641 catgcagcgc gcgctggtgg aggtggccgc gcgcaacgtc gagaccatcc tgcccggctt
    11701 cacgcacatg caggtggccc agccggtgag cttcgcgcac cacctgctcg cctacgtgga
    11761 gatgttcgcg cgcgacgagg aacgcctggc cgacgtgcgc cgccgcgtga accgcctgcc
    11821 gctgggcgcc gcggcgctgg ccggcaccag ctatccgctc gaccgcgagc gcgtcgcgcg
    11881 cacgctgggc ttcgacggcg tgtgccagaa cagcctggac gcggtgagcg accgcgactt
    11941 cgcgctcgag ttcgcgtcct tcgcggccat cgcgatggtg cacgtgtcgc ggctggccga
    12001 ggagatcgtg ctctggatga gccagaactt cggcttcatc gacctggcgg accgctactg
    12061 caccggctcg tcgatcatgc cgcagaagcg caaccccgac gtcgccgagc tggcgcgcgg
    12121 caagagcggc cgcgtggtgg gtcacctcat gggcctgatc acgctgatga agggccagcc
    12181 gctcgcctac aacaaggaca accaggaaga caaggagccg ctcttcgaca ccgtcgacac
    12241 cctgcgcgac acgctgcgca tcatggccga gatggtgggc ggcatcacgg tcaaggccga
    12301 ggcgatggag cgggcggcgc tgaagggcta cgccaccgcc accgatctcg ccgactacct
    12361 ggtgaagaaa ggcctgccg
//
LOCUS       DASRIE010000221         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1279137, whole genome shotgun sequence.
ACCESSION   DASRIE010000221 DASRIE010000000
VERSION     DASRIE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279137"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..521
                     /locus_tag="VFB53_09500"
     CDS             108..521
                     /locus_tag="VFB53_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN87003.1"
                     /translation="MAHLRTYIVEDSPVIRENLIATLEEMVPVEVIGTAEDESSAAQW
                     LGKSDNACDLVIIDIFLKSGSGLGVLQTARKAAAQRKLVVLSNYATPDMRRKCLQLGA
                     DKVFDKSNEIDGLINYCARLASGEETGNGVGARPS"
     gene            complement(591..1223)
                     /locus_tag="VFB53_09505"
     CDS             complement(591..1223)
                     /locus_tag="VFB53_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN87004.1"
                     /translation="MIRVAIVDDHAIVRAGLRQYFQDQVDLKVTGEAANGREALDLVR
                     KGEVDVLVMDLSMPDQSGVDALAAIKARAPELPVLILSGFPEEHYATTLLRQGAAGYL
                     NKECDPEEIVKAIRTVHRGRKYITPGVAELLADGLSGAGEKPPHEQLSEREFQVFLRL
                     AKGETIGHMADSMSLSVKTVSTYRTRVMEKMKLSSNSDLTYYALKNGLIQ"
     gene            1258..2670
                     /locus_tag="VFB53_09510"
     CDS             1258..2670
                     /locus_tag="VFB53_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZN87005.1"
                     /translation="MQDQAVNILRRLKRSPFAFPLATLAALAMIFISEGAYWQSTRSM
                     RELGDMGAARLQLTNLSRRVADAESGQRGYLLTGRTEYLQPYRGAFADIDQALVWLRG
                     YYARNPDHAQAMAKLETLVQRKLSELDATMRLHDEGKTEAWRAVLLTDIGKEQMQEIR
                     NISEQLLSEEGRKEAAGRSKVDRTLLLNRIGVAAMTAMSLLALAMYLRQAAALDAHRR
                     ERQQAIEAERDQLEVEVARRTAQLTELAQQLTELAQHLQTAREDERSRLARELHDELG
                     ALLTAAKLDAARIKSRLGQNAPEAVERLNHLNETLNNGIALKRRIIEDLRPSSLANLG
                     LIAALEIQAREFAERTGLEIDCRLEPVPLKPSAELTAYRLVQEAFTNIAKYAQAKRVQ
                     VSLLPVDGCAEISVGDDGVGFDPHLRRPSAHGLLGMRYRVAAEGGRFELESAPGGGTR
                     IRATLPLLRAPLEQAQQEAA"
     gene            2949..3320
                     /locus_tag="VFB53_09515"
     CDS             2949..3320
                     /locus_tag="VFB53_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87006.1"
                     /translation="MMSTKPFGQSSIADQAAQGADSAIGRTQQMADEALSSLSDKVHD
                     MRGQAAPVINRVAAKAEELARRSADAMRDRSQLIKEQAYRAQDMTVGYIKDEPLKSIL
                     MAAAAGAALMALLQLLRTRDH"
     gene            3398..3787
                     /locus_tag="VFB53_09520"
     CDS             3398..3787
                     /locus_tag="VFB53_09520"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZN87007.1"
                     /translation="MIHPLFRLLVSEPQMLADHVEAYAELVTEEVGTVAAQMKRRALL
                     TAAGGVCLLLALLFAAIALMLWGSAPDGAMRAPWVLWVVPGVPLAIGAACLLMARKKD
                     EGAHGVQAIKQQFAEDLAMLRSVRAAS"
     gene            3840..4574
                     /locus_tag="VFB53_09525"
     CDS             3840..4574
                     /locus_tag="VFB53_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87008.1"
                     /translation="MSAFVPDSSVPADGAEAQAAAEAEAPEERGSAAERLAASRERMR
                     QWMMQTDRRGASRERMEAAMAAGEQPALADRLRHAPVIGVLIDALGAWWADHPLQPAV
                     VIAQGVVNRRITPVVRRHPVPILLGAFLVGVLLVRLRPWRWIAKPALFAGLGSQLVSR
                     VVSAVPMDKVLAALGALAGFQAASGGEPKLTDPDLSAQAAAAAAAAPPGTAPSTGGAT
                     PSGGAAPGASSPPVPRAAVEAETVTP"
     gene            4722..>4897
                     /locus_tag="VFB53_09530"
     CDS             4722..>4897
                     /locus_tag="VFB53_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZN87009.1"
                     /translation="MDDKTRLALDDAALEAAKRSLDDGVVTPSYGPWRDDIVKLLNDA
                     LATELVCVLRYKRH"
BASE COUNT          742 a         1778 c         1711 g          666 t
ORIGIN      
        1 ctccaggtac aagctcgtcg tcccgccctc cctcacgagt gtccgtcccg gactctgcga
       61 ccgagcgccg cgacccgtcc ggagtcatgc ccttgcaatc gtccgtcatg gcgcacttga
      121 gaacctacat cgtcgaagac agtccggtga tccgcgagaa cctgatcgcg acgctggagg
      181 agatggttcc cgtggaggtc atcggcaccg ccgaggacga gtcgagcgcg gcgcaatggc
      241 tcggcaagtc ggacaacgcc tgcgacctgg tcatcatcga catcttcctc aagagcggct
      301 cgggcctcgg cgtgctgcaa accgcgcgca aggcggccgc gcagcgcaag ctcgtggtgc
      361 tcagcaacta cgcgacgccc gacatgcgcc gcaagtgcct gcagctcggc gcggacaagg
      421 tgttcgacaa gtccaacgag atcgacggcc tgatcaacta ctgcgcgcgc ctggcctcgg
      481 gcgaggagac gggcaacggg gtgggcgcgc ggccgtcgtg acggccgccg cacgggcgcg
      541 cggcggcgcg cattcccgcg gcgccgcgcc gcgcggggcg ccgcggcgcc tcactggatc
      601 aggccgttct tcagcgcgta gtaggtcagg tcgctgttgg acgacagctt catcttctcc
      661 atcacgcggg tgcgataggt gctgacggtc ttgacgctga gcgacatgct gtcggccatg
      721 tggccgatgg tctctccctt cgccagccgc aggaacacct ggaactcgcg ctcggacagc
      781 tgctcatgag gcggcttctc gccggcgccg gacagcccgt ccgccagcag ctccgccacg
      841 ccgggggtga tgtacttgcg cccgcggtgc accgtgcgga tcgccttgac gatctcttcc
      901 gggtcgcatt ccttgttgag gtagccggcc gcgccctgcc gcagcagcgt cgtcgcgtag
      961 tgctcttcgg gaaagcccga aaggatcagc acgggaagct ccggggcgcg ggccttgatg
     1021 gccgccaggg catccacccc gctctggtcc ggcatggaca ggtccatcac cagcacgtcc
     1081 acctcgccct tgcggacgag gtccagcgcc tcgcggccgt tcgccgcctc gccggtcacc
     1141 ttcaggtcga cctggtcctg gaagtactgc cgcaggcccg cccggacaat ggcatggtcg
     1201 tcgacgatgg ctacacggat catggggtcc acgcgtgaag tcgatgagcg agtttagatg
     1261 caagaccaag ccgtgaacat cctccgccgc ctcaagcgca gcccgttcgc gtttcccctg
     1321 gcgacgctcg ccgcactcgc gatgatcttc atcagcgagg gcgcgtactg gcagtcgacg
     1381 cgctcgatgc gcgagctggg cgacatgggc gccgcccgcc tgcagctcac caacctgtcg
     1441 cgccgcgtgg ccgacgccga gagcggccag cgcgggtatc tcctcaccgg ccgcaccgag
     1501 tacctgcagc cctaccgcgg cgcgttcgcc gacatcgacc aggcgctcgt gtggctgcgc
     1561 ggctactacg cgcgcaaccc cgaccatgcg caggcgatgg ccaagctcga gacgctcgtg
     1621 cagcgcaagc tgtcggagct cgacgccacc atgcgactgc acgacgaagg caagaccgaa
     1681 gcgtggcgcg cggtgctgct caccgacatc ggcaaggagc agatgcagga gatccgcaac
     1741 atctcggagc agctcctgag cgaggaaggc cgcaaggagg cggccgggcg cagcaaggtg
     1801 gaccgcacgc tgctgctcaa ccgcatcggc gtcgcggcga tgacggcgat gagcctgctc
     1861 gcgctggcga tgtacctgcg ccaggccgcc gccctcgatg cgcaccgccg cgagcgccag
     1921 caggcgatcg aggccgagcg cgaccagctc gaggtggaag tggcccgccg caccgcgcag
     1981 ctcaccgagc tcgcgcagca gctcaccgag ctggcgcagc acctgcagac cgcgcgcgag
     2041 gacgagcgca gccgcctcgc gcgcgagctg cacgacgagc tgggcgcgct gctcacggcg
     2101 gccaagctcg acgccgcgcg catcaagtcg cgcctcggcc agaacgcacc cgaagcggtg
     2161 gagcggctca accacctcaa cgagacgctg aataacggca tcgcgctcaa gcgccgcatc
     2221 atcgaggacc tgcgcccctc gtcgctggca aacctcgggc tcatcgcggc gctcgagatc
     2281 caggcgcgtg aattcgccga gcgcacgggc ctggagatcg attgccggct cgagcccgtg
     2341 ccgctgaagc cttcggccga gctcacggcc taccggctgg tgcaggaagc gttcaccaac
     2401 atcgccaagt acgcgcaggc gaagcgcgtg caggtgtcgc tgctgcccgt ggacggctgc
     2461 gccgagatct cggtgggcga cgacggcgtg gggttcgacc cgcacctgcg ccgcccgtcc
     2521 gcgcacgggc tgctcggcat gcgctaccgc gtggccgccg aaggcggtcg cttcgagctc
     2581 gagtcggcac ccggcggcgg cacgcgcatc cgcgccacgc tgccgctgct gcgcgcgccg
     2641 ctggagcagg cgcaacagga ggccgcgtga acgcgcccgc cgcgtgggcg cggtcctaca
     2701 cgacatcgct cgctgcaccg acaccgccgc gggcgaccct cccaccaact tggatacgcc
     2761 ccctccataa gcttgctcat cgcggcgcga aatggcgcgt cgcgaccgcg gccgcccgtt
     2821 ccggccggcg ggcacgcacg aggagacgtg acccacagag agcacttcgt cgccgtcttc
     2881 gcgatcgcgc cgatcgccgt gcgccgtcca cccaccacac cacccaccgc cccaccgaaa
     2941 ggatgaacat gatgtccacc aagccgtttg gccaatccag catcgccgac caggccgcgc
     3001 agggcgcgga cagtgccatc ggccggaccc agcagatggc cgacgaagcg ctgtccagcc
     3061 tgtcggacaa ggtccatgac atgcgcggcc aggccgcccc ggtgatcaac cgcgtcgcgg
     3121 ccaaggcgga ggagctggca cgccgcagcg ccgacgcgat gcgcgaccgc tcgcagctga
     3181 tcaaggagca ggcgtaccgg gcccaggaca tgacggtcgg ctacatcaag gacgagccgc
     3241 tcaagtcgat cctgatggcc gcggccgccg gcgccgcgct gatggcgctg ctgcagctgc
     3301 tgcgcacgcg cgaccactga tccgcgtcgc cgccgccgcc acggggtcgc ccggtgcgcg
     3361 accccgaggc tccctttccg tttgcggagc ccctgccatg atccacccgc tctttcgcct
     3421 gctcgtttcc gaaccccaga tgctggccga ccacgtggag gcctatgcgg aactggtgac
     3481 cgaagaggtc ggcaccgtcg ccgcacagat gaagcgacgc gcgctgctga cggccgcagg
     3541 cggcgtgtgc ctgctgctcg cgctgctgtt cgcggccatc gcgctgatgc tgtggggttc
     3601 ggcgccggac ggcgccatgc gggcgccgtg ggtgctgtgg gtggtgccgg gcgtgccgct
     3661 ggcgatcggc gccgcgtgcc tgctcatggc gaggaagaag gacgaagggg cgcacggcgt
     3721 gcaggccatc aagcagcagt tcgccgagga cctggcgatg ctgcgcagcg tgagggccgc
     3781 atcgtgagcc gggcgcgtgg ccgcaccggc gccggcgttc gcgccacgga ggccgggcga
     3841 tgagcgcctt cgttcccgac tcgtccgtcc cggccgacgg ggccgaggcg caggcggccg
     3901 ccgaagccga agcccccgag gaacgggggt ccgccgccga gcggctcgcc gcctcgcgcg
     3961 agcgcatgcg ccagtggatg atgcagaccg accggcgcgg cgcctcgcgc gagcgcatgg
     4021 aggcggcgat ggcggccggc gagcagcccg cgctggccga ccggctgcgt catgcgccgg
     4081 tcatcggcgt gctgatcgac gcgctcggcg cctggtgggc cgaccatccg ctgcagcccg
     4141 ccgtggtgat cgcgcagggc gtcgtcaacc ggcgcatcac gccggtcgtg cggcgccatc
     4201 cggtcccgat cctgctgggc gccttcctgg tgggcgtgct gctggtgcgc ctgcgcccgt
     4261 ggcgctggat cgccaagccg gcgctgttcg ccgggctggg ctcgcagctc gtctcgcgcg
     4321 tcgtgtccgc cgtgccgatg gacaaggtgc tggcggcgct cggcgccctg gcgggattcc
     4381 aggcggcaag cggcggcgag ccgaagctga ccgatcccga cctgtctgcg caggcggcgg
     4441 cggccgccgc cgcggcgccg cccggcacgg cgccgtcgac cggcggggcg acgccctccg
     4501 gcggggccgc gccgggcgcg tcgtcgccgc cggttccgcg cgccgcggtc gaggccgaga
     4561 ccgtcacgcc ctgacggatc gccgccaacg cggtttcgtc ctacatgggc agaagccgcg
     4621 ttccgatggc tcccgcaagg ggctgcccct agagtttgtg acaacgaggc gcgaaggccg
     4681 acgcgcggcg cgctcgtgcc gattccagga gatttcgata catggacgac aagaccagac
     4741 tggcgctgga cgacgcggcc ctcgaggcgg ccaagcgcag cctcgacgac ggcgtggtca
     4801 ccccgagcta cgggccgtgg cgcgacgaca tcgtgaagct gctcaacgac gcgcttgcca
     4861 ccgagctcgt ctgcgtgctg cgctacaagc gccacta
//
LOCUS       DASRIE010000222         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2843965, whole genome shotgun sequence.
ACCESSION   DASRIE010000222 DASRIE010000000
VERSION     DASRIE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843965"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFB53_09535"
     CDS             <1..811
                     /locus_tag="VFB53_09535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN87010.1"
                     /translation="IVFSGDLGQPGLPVVRDPTPIDEADVLLVESTYGNRDHKNLPQT
                     LDEFAYALNDTLASRRGNVVIPAFAVGRTQDVLHYMAQLQKERRLPARMEVYVDSPMA
                     LAATRITLKYARLPELERVRFTEDLEESKRINAIAAGAVIISASGMCEGGRIRHHLRY
                     NISRPECAVVFTGFQAAGTLGRRIVDGAQSVRLFGDDYPVRARIFTINGLSAHADRSA
                     LLGWLGHFRRAPDRTWVVHGETLPAQTLCEAINAKPGWRAAVPSPGQSVEL"
     gene            complement(847..2442)
                     /locus_tag="VFB53_09540"
     CDS             complement(847..2442)
                     /locus_tag="VFB53_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZN87011.1"
                     /translation="MLLRYGFGDAVQRLGIALHRREAGEEAGLDGPQRARRALQDLGP
                     AFVKLGQILATRVDLFPPEWIAELEKLQDRAPASPWPEVRAQLAEDLGAPPEEVFAAF
                     EPQPIAAGSIAQVHRAQLPDGTPLAVKVRRPGIVPVVEADLRLLARLAETLEARFPEL
                     ARFRPREVVRQFTRTLRTELDLAAECHNAERVAANFRDRPEIVVPKVYWEWSGERVNV
                     QEYIDGIPSRDPEALAAAGLERKVLAHRGAQAMLKMVLEDGFFHADPHPGNVFYLPGD
                     RVAFIDFGMVGHLSERRRDELVRLLDGLVGRDAEGAVDILLEWAGDAQVDADKLSLDV
                     DAFIDRYHGRPLHELRMGAMLADLIALLREHRLALPPDLALVLKACVTLETYGRELDP
                     GFHMVAHAEPFLRAATLERYGAAKLARRGWRAGRLLLDTLAELPRDLRELVRAARNGR
                     LQVRVDLTSLARLGHQLDSAANRLAIGIVTAALIVGSAIVMTVEGGPALAGLPFFGLL
                     GFLGALVGGAWLFVSILRSGGAR"
     gene            2673..2957
                     /locus_tag="VFB53_09545"
     CDS             2673..2957
                     /locus_tag="VFB53_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87012.1"
                     /translation="MLSLEDCLALCELSEEEVLAIAHHERIPEIAAAELGNYLVHTPE
                     GEMRIKTIIRDDIAEACACGDRLRELALRVMLRDFVRQHPRCEERHKARP"
     gene            complement(2987..3661)
                     /locus_tag="VFB53_09550"
     CDS             complement(2987..3661)
                     /locus_tag="VFB53_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014726034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZN87013.1"
                     /translation="MDQIVRRPPGRISTQVNARRGDARSGRLAREYFNAEQRMRERGV
                     RRTILVMGSTRVSRGNPYYAVAREFGARVAADGRAALMTGGGPGIMEAANRGAYEAGG
                     LSIGLNIRLPREQAANRYLTPGLRFSFRYFALRKLHFLLRARAMVAFPGGFGTFDELF
                     ETLTLVQCRKIAPMPIVLVGEKYWRRAVDLDFLVAEGMIAKSDRRLYRYADTAAQIWR
                     AVRRAL"
BASE COUNT          621 a         1448 c         1368 g          542 t
ORIGIN      
        1 gatcgtgttc tccggcgacc tcggccagcc gggcctgccc gtggtgcgcg atccgactcc
       61 gatcgacgag gccgacgtgc tgctggtcga gtccacctac ggcaaccgcg accacaagaa
      121 cctgccgcag accctggacg agttcgccta cgcgctgaac gacacgctgg cgagccggcg
      181 cggcaacgtc gtcatcccgg ccttcgccgt cggccgcacg caggacgtcc tgcactacat
      241 ggcacagctg cagaaagagc ggcgcctgcc cgcgcgcatg gaggtctacg tcgactcgcc
      301 gatggcgctc gcagcgacgc gcatcacgct gaagtacgcg cggctgcccg agctcgagcg
      361 cgtgcgcttt accgaggacc tggaggagtc caagcgcatc aacgccatcg cagccggcgc
      421 ggtcatcatc tccgcgagcg gcatgtgcga aggcgggcgc atccgccacc atttgcgcta
      481 caacatctcg cggccggagt gcgcggtggt gttcacgggc ttccaggccg ccggcacgct
      541 cgggcggcgc atcgtcgacg gcgcccaatc cgtgcgcctg ttcggcgacg actatcccgt
      601 gcgcgccagg atcttcacca tcaacggcct ctccgcgcac gccgaccgct cggcgctgct
      661 cggctggctg ggccatttcc gccgcgcgcc ggaccggacc tgggtagtgc acggcgaaac
      721 cctgccggcg cagacgctat gcgaggcgat caacgccaag cccggctggc gcgccgcggt
      781 gccgtccccg ggacagagcg tcgagctttg agttacggca gcccgaaagc acgatcacta
      841 gagcgctcag cgcgcgccgc cgctccgcag gatggataca aagagccagg cgccgcccac
      901 cagcgcgccg agaaaaccga gcaggccgaa aaacggcagc ccggcgagcg caggcccgcc
      961 ctccaccgtc atgacgatcg ccgagccgac gatgagcgcg gcggtgacga tgccgatcgc
     1021 gagacggttc gccgcgctgt ccagctggtg gccgaggcgc gcgaggctgg tcaggtccac
     1081 gcgcacctgc aggcggccgt ttcgcgccgc gcgcaccagc tcgcgcaggt cgcgcggcag
     1141 ctcggcgagc gtgtccagca acagccggcc cgcgcgccag ccgcgccgcg ccagcttcgc
     1201 cgcgccgtag cgctcgagcg tcgcggcgcg caggaacggc tcggcgtgcg ccaccatgtg
     1261 gaagcccggg tcgagctcgc ggccgtaggt ttcgagggtc acgcacgcct ttagcacgag
     1321 cgcgaggtcg ggcggcagcg cgaggcggtg ctcgcgcagc agcgcgatca ggtcggcgag
     1381 catcgcgccc atgcgcagct cgtgcagcgg ccggccgtga tagcggtcga tgaaggcgtc
     1441 gacgtcgagc gagagcttgt ccgcgtccac ctgcgcgtcg cccgcccatt cgagcaggat
     1501 gtcgacggcg ccctcggcat cgcgccccac caggccgtcg agcagccgca ccagctcgtc
     1561 gcgccggcgc tccgaaagat gccccaccat gccgaagtcg atgaacgcga cccggtcgcc
     1621 ggggaggtag aacacgttgc ccgggtgcgg gtcggcgtgg aagaagccgt cctccagcac
     1681 catcttgagc atcgcctgcg cgccgcggtg cgcgagcacc ttgcgctcga gccccgccgc
     1741 cgccagcgcc tcggggtcgc ggctcggaat gccgtcgatg tactcctgca cgttgacgcg
     1801 ctcgccgctc cactcccagt acaccttggg caccacgatc tcgggccggt cgcggaaatt
     1861 cgcggcgacg cgctcggcgt tgtggcattc ggcggcgagg tcgagctccg tgcgcaaggt
     1921 gcgcgtgaac tggcgcacca cttctcgcgg ccggaagcgg gcgagctcgg gaaagcgcgc
     1981 ttcgagcgtc tcggcgaggc gggcgaggag ccgcaaatcg gcctccacca ccggcacgat
     2041 gcccggtcgg cgcactttga cggcgagcgg cgtgccgtcg gggagctgcg cgcggtgcac
     2101 ctgcgcgatc gacccggcgg cgatcggctg cggctcgaac gcggcgaaca cctcctccgg
     2161 cggcgcgccg agatcctcgg cgagctgcgc gcgcacctcg ggccagggcg aggccggcgc
     2221 acgatcctgc agcttttcca gctcggcgat ccactccggc gggaaaaggt ccacccgcgt
     2281 ggcaaggatc tggccgagct tgacgaaggc cgggcccaga tcctgcaggg cgcgccgcgc
     2341 gcgctgcggg ccgtcgagcc cggcctcttc gccggcttcc cggcgatgca gcgcgatgcc
     2401 cagccgttgc accgcgtcgc cgaacccgta gcgcagcagc acggcgccga tctcccgtac
     2461 gcgggggagg tcgcgtgcag tttgggcgat cgcgcgcagc atcattgtct ccttgccgaa
     2521 tcgtaggcag cgcggccacg acacctttga ccgggatcaa aaaccggcgc gggcatgtca
     2581 ttacgctaag gacatggagg ccgacaccct ccacgaccgg gtcacgccct agctcgaccg
     2641 ccgctggccg cacgcaaaac tgaaaggaca ccatgcttag cctggaagac tgcctcgcgc
     2701 tctgcgagct ttccgaagag gaagtcctcg ccatcgcgca ccacgagcgc attccggaga
     2761 tcgccgccgc cgagctcggc aactatctcg tgcatacgcc cgaaggcgag atgcgcatca
     2821 agaccatcat ccgcgacgac atcgccgagg cgtgcgcgtg cggcgatcgc ctgcgcgagc
     2881 tcgcgctgag ggtgatgctg cgcgatttcg tccggcagca cccccgctgc gaagagcgcc
     2941 acaaggcccg cccatgaccg ccaatccaag gcggggctag gtctcctcag agcgcgcggc
     3001 gcaccgcgcg ccagatctgc gccgccgtat cggcgtagcg atagagccgc cggtcgcttt
     3061 tcgcgatcat cccctcggcg acgaggaagt ccagatcgac ggcgcggcgc cagtactttt
     3121 cgcccaccag cacgatcggc atcggcgcga tcttgcggca ctgcacgagg gtcagcgtct
     3181 cgaacagctc gtcgaacgtg ccgaaaccgc cgggaaacgc caccatggcg cgcgcgcgca
     3241 gcaggaaatg cagcttgcgc agcgcgaagt agcggaagct gaagcgcagg cccggcgtga
     3301 ggtagcggtt cgcagcctgc tcgcgcggca gccggatgtt gaggccgatc gacaagccgc
     3361 ccgcctcgta tgcgccgcgg ttcgccgctt ccatgatccc ggggccgccg ccggtcatga
     3421 gcgccgcccg gccgtcggcc gcgacccgcg cgccgaactc gcgggccacg gcgtagtacg
     3481 ggttgccgcg cgacactcgc gtgctgccca tcaccaggat ggtgcgccgc acgccgcgct
     3541 cgcgcatgcg ctgctcggcg ttgaagtact cgcgcgcaag gcgcccgctg cgcgcgtcac
     3601 cgcgacgggc gttgacctgc gtcgagatgc gccccggcgg ccggcgcact atctgatcca
     3661 tcaaaaacct cccgggtggc gatcgaccag acgctcggcg agtgcatgcg cgagcgcaag
     3721 tgcgcgcagg tgcagtgtcc gctcgccgca ttgctgaagc gcgatgaggc gcgagttcct
     3781 gggcgacacg ccgttcgacc tgcgctgagc tacgcaggcc tgcgccttgc gccatgatgt
     3841 gagctgcgcc gctagcgcgg cgcgtgctgc ttgacgtccc cgcgctgcac gcgcccgtag
     3901 tcttccagac gccggcgtgc ggcgtcgaac gcctcgcgca gcgcgacgtg cacatcctcg
     3961 tcgtgctcgt gcgtgacgg
//
LOCUS       DASRIE010000223         6915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3333790, whole genome shotgun sequence.
ACCESSION   DASRIE010000223 DASRIE010000000
VERSION     DASRIE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333790"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFB53_09555"
     CDS             <1..468
                     /locus_tag="VFB53_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN87014.1"
                     /translation="LANPAATCVLHTHMPHATALTLLEGGRLEMVEQNALRFHDDIAY
                     DDIYNGLVVDDAEGDRLARVLGTKRVLFLANHGVIVVGPTVSEAYDLLYYLERACRLQ
                     VLARATGLPFRKVRPEVVGETYRMILADTPKYAGAHFAALKRLLDREEPDYAG"
     gene            470..991
                     /locus_tag="VFB53_09560"
     CDS             470..991
                     /locus_tag="VFB53_09560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN87015.1"
                     /translation="MEVRAAAQGDFAAIHAIYAHHVLHGLASFEEVPPDLQEMRRRHR
                     EIAERGLPYLVAEERGEILGYGYCAPYRSRSAYRYALEDSIYIKEGHLGKGIGARLLG
                     ELLRLCEGLGYRQVVAVIGDSGNAASIGLHARHGFLRVGTLRSTGYKFGRWVDSVLMQ
                     RPLGAGDGTPPAT"
     gene            complement(984..1748)
                     /locus_tag="VFB53_09565"
     CDS             complement(984..1748)
                     /locus_tag="VFB53_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN87016.1"
                     /translation="MISALAEALSSLASPWVLAACTAVVFAAYVLRGATGFGAGVVAI
                     PLLALAMPLNVVIPVVTTMGILASLGQSIQEIRHVDWRALRGLALPSAIGLGFGLWLF
                     ASLDQALLLRVFAGFIIAYGLWSLLPRAPAAALPPRALAAAVGGAGGLVATLFGGMAG
                     PFYAIYLKALNLDKRRFRASMSSVLLCLGLVRAGGYGGLGFFDRRAIAALALFAPVMV
                     LAMLAGDRWHARLEQEKFERMVAVLLVMSGVALLLK"
     gene            complement(1741..2721)
                     /locus_tag="VFB53_09570"
     CDS             complement(1741..2721)
                     /locus_tag="VFB53_09570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZN87017.1"
                     /translation="MKALLCKEFGPPESLVLEDVPSPQAGAGEVVVSIKAASVNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKEAGPGVTGVKPGDKVIAFTTFGAFAEEVKTEA
                     ARLLPLPEGMSFEEGASFILTYGTTDHALRDRAQMKSGETLLVLGAAGGVGVAAIEIG
                     KAMGARVIACASSDEKLAVCRQHGADEAINYATEDLRERIKTLTGGKGVDVIYDAVGG
                     PYSEPAFRSIAWRGRHLVIGFAAGDIPKLPLNLALLKGASVVGVFWGDFTRREPQVFA
                     ESARQLGRWYREGKLKPHVSATFPLARAAEAIKLLASRKAKGKVVIRIGD"
     gene            complement(2733..3467)
                     /locus_tag="VFB53_09575"
     CDS             complement(2733..3467)
                     /locus_tag="VFB53_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HZN87018.1"
                     /translation="MDLNLKGKRALVTGGSKGIGRACAEVLAAEGCSVLVAARRPQPG
                     PQAKAIDLSQRGAAEALAAWAGDLDILVNNAGAIPGGDLLRVDEDTWRNAWDLKMFGY
                     LNLTRQVYARMKARKAGVIVNVLGNAGEKLDASYIAGSTANAGLMAFTRALGGASHAD
                     GIRVVGVNPGPVATDRLKGLFRQRAEAKFGDPERYPELFAGMSFGRPAAPEEIAWMVA
                     FLASERSAYTTGCIVTIDGGLASRSA"
     gene            3487..4323
                     /locus_tag="VFB53_09580"
     CDS             3487..4323
                     /locus_tag="VFB53_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN87019.1"
                     /translation="MPLITTDDGVKLHYEESGSGTPVVFVHEFAGDLRSWDPQLSHFS
                     RRYRCIAFNARGYPPSEVPESFERYSQERARDDVLAVMDALKIQRAYVVGLSMGGFAA
                     LHFGIKYSARALGLVVAGCGYGAEPAKREEFQRASRDNAKALLERGMAAFAAGYGHNA
                     ARLQLRAKDPRGFALYIKYLTEHSALGSANTMLGYQARRPSLYDLTAQMQGVTAPTLV
                     VAGDEDDATLEPGLLMKRTIPRCGLVVLPWSGHALNLEDPALFNRVVEDFFHQVDNGR
                     FA"
     gene            4320..5108
                     /locus_tag="VFB53_09585"
     CDS             4320..5108
                     /locus_tag="VFB53_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN87020.1"
                     /translation="MSAGRLGVEQRGAVGWIVFDQPQKKNAINGAMWRGVPEAMARFD
                     ADAAIRCIAFRGAGAEAFAAGADISEFEKVRAGGGAVSEYDGLLDRVLHSIQDSRKPS
                     VALIHGFCMGGGLEVALACDLRYCGASAQFAIPAAKLGIAYNVEGHKRLLETVGHARA
                     REIMFLGRRYGAAEALAMGLVHRVLPDAELDAYVDGVIATLLENAPLAIANSKTILEE
                     YAKAQGAADQGRMQAAIERCARSEDYQEGRRAFMEKRKPRFEGK"
     gene            5105..6190
                     /locus_tag="VFB53_09590"
     CDS             5105..6190
                     /locus_tag="VFB53_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN87021.1"
                     /translation="MSLRIKSVRVRAVAAPMKRPLATSTGALTVSPLLLVDLKTDVGI
                     VGRSYLFGIGAQNLRPIAALLEAMAGMLQGDAVAPFDIEAKLRKRYTLLGLHNIVLFA
                     MSGIDMAAWDALGQSLEQPLVRLLGGAPRPVPAYNSKGLGIMPLKPLVKEAGELVAEG
                     FGAVKLRLGRPAAADDLEAVRAVKRAIGPKVKLMVDFNQGLTVVEAIKRGRMIDAEGG
                     VLWIEEPVRADDFAGNARIAREVATPIQIGENFMGPEQMAQALAAGACDYAMPDAQRI
                     GGVTGWMRAAALAQAAGMEMSSHLFPEVSCHLLAVTPTCHWLEYVDWADPVLEEPTRL
                     KDGHVLVPERPGIGMRWNEKAVKRYAL"
     gene            6190..>6915
                     /locus_tag="VFB53_09595"
     CDS             6190..>6915
                     /locus_tag="VFB53_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN87022.1"
                     /translation="MFTRHHAHWPPGVPKTLSVPRTSLYYNLEVSARRYPGKAAIYYY
                     GTTLSYAELQRSAEALAGYLQRACGVRRGDRVLLYAQNSPQFVIGYYAILRADAVVVP
                     VNPMNKRDELAYYVGDSDARVVVSTQDLYGEIEPHLGKGLAHCVLGTYSDYLREATDL
                     NVPEFVRAPRAAAANWQAALAAGHRPDAHGTGPEDLAVLPYTSGTTGHPKGTMLTHAN
                     LLATSTGACAWIQSSSEAVVLGVL"
BASE COUNT         1057 a         2435 c         2460 g          963 t
ORIGIN      
        1 ctcgcgaatc ccgcggccac ctgcgtactg cacacgcaca tgccgcacgc gaccgcgctc
       61 acgttgctcg aaggcggccg gctcgaaatg gtcgagcaga atgcgctgcg cttccacgac
      121 gacatcgcct acgacgacat ctacaacggc ctggtggtgg atgacgccga gggcgaccgc
      181 ctggcgcgcg tgctcggcac gaagcgcgtg ctgttcctcg ccaaccacgg cgtgatcgtg
      241 gtcgggccga cggtgtccga ggcctacgac ctgctttact acctcgagcg cgcctgccgg
      301 ctgcaggtgc tggcgcgcgc caccgggctg cccttccgca aggtgcgccc cgaggtggtg
      361 ggcgagacct accgcatgat cctcgccgac acgcccaagt acgcaggcgc gcactttgcc
      421 gcgctcaagc gcctgctgga ccgcgaagaa cccgactacg cgggctgaag tggaagtccg
      481 tgccgccgcg cagggcgatt tcgctgcgat ccatgccatc tacgcgcacc acgtgctgca
      541 cgggctcgcc agcttcgagg aggtgccgcc cgacctgcag gaaatgcgcc gtcgccaccg
      601 ggagattgcc gagcgcggcc tgccctacct cgtggcggag gagcgcggcg agatcctcgg
      661 ctacggctac tgcgcgccct atcgctcgcg ctcggcctac cgctacgccc tggaggactc
      721 catctacatc aaggaagggc acctgggcaa aggcatcggc gcgcgactcc tgggcgagct
      781 gctgcggctg tgcgaggggc tcggctaccg ccaggtggtc gcggtgatcg gcgacagcgg
      841 caacgcggcg tccatcgggc tgcacgcgcg gcacggcttc ctgcgcgtcg gcacgctgcg
      901 ctccacgggc tacaagttcg gccgctgggt ggacagcgtg ctgatgcaaa ggccgctggg
      961 cgccggcgac ggcacgccgc cggctacttg aggagcaggg cgactccgct catcaccagc
     1021 aggacggcga ccatgcgctc gaacttctcc tgctcgaggc gcgcgtgcca gcggtcgccg
     1081 gcgagcatgg cgagcaccat caccggcgcg aacagggcga gcgccgcgat cgcgcgccgg
     1141 tcgaagaagc ccagcccgcc gtagcctccg gcgcgcacca gccccaggca cagcagcacc
     1201 gaggacatgc tggcgcggaa gcgccgcttg tccaggttga gcgccttcag gtagatggca
     1261 tagaacgggc cggccatgcc gccgaacagc gtggccacca gaccgcccgc gccgcccacc
     1321 gccgcggcga gggcccgcgg gggcagggcg gcggccggcg cgcgcggcag gagcgaccac
     1381 aggccgtagg cgatgatgaa gccggcaaag accctgagca gcagggcctg gtccagcgag
     1441 gcgaacagcc acaggccgaa acccagcccg atcgccgacg gcagcgccag cccgcggagc
     1501 gcgcgccaat ccacatggcg gatctcctgg atcgactggc cgagcgaggc gaggatgccc
     1561 atcgtcgtga ccaccgggat gacgacattg agcggcatcg ccagcgccag cagcggaatg
     1621 gcgacgacgc cggcgccgaa gccggtcgcg ccgcgcagca cgtaggcggc aaagaccacc
     1681 gccgtgcagg cggcgaggac ccagggggag gcgaggctgg aaagcgcctc ggccagggcg
     1741 ctaatcaccg atgcggatga cgaccttgcc ctttgccttg cgcgacgcca gcagcttgat
     1801 ggcttccgcc gccctggcca gcgggaaggt ggccgacacg tggggcttga gcttgccctc
     1861 gcggtaccag cggccgagct ggcgcgcgct ctcggcgaat acctgcggct cgcgccgggt
     1921 gaaatcgccc cagaacacgc cgaccacgga ggcgcccttg agcagcgcga ggttgagcgg
     1981 cagcttcggg atgtcgcccg cggcgaagcc gatcaccagg tgccgcccgc gccaggcgat
     2041 ggagcggaac gcgggctcgc tgtaggggcc gccgacggcg tcgtagatca catccacgcc
     2101 cttgccgccg gtgagcgtct tgatgcgctc gcgcaggtcc tcggtggcgt agttgatcgc
     2161 ctcgtcggcg ccatgctgcc ggcacacggc cagcttctcg tcgctcgagg cgcaggcgat
     2221 gacgcgcgcg cccatcgcct tgccgatctc gatcgcggcg acgcccacgc cgccggcggc
     2281 gcccagcacc agcagcgttt cgccggattt catctgcgcg cggtcgcgca gcgcatggtc
     2341 ggtggtgccg taggtgagga tgaaggaagc gccttcctcg aagctcatgc cctcgggcag
     2401 cggcagcagc cgcgcggcct cggtcttcac ttcctcggcg aaggcgccga aggtggtgaa
     2461 ggcgatcacc ttgtcgccgg gttttacgcc ggtcacgccg ggcccggcct ctttcaccac
     2521 gccggcgagc tcgctccccg gggaaaaagg cagcggcggc ttgaactggt acttgttctg
     2581 gatgatcagc acgtcgggga aattcacgct cgccgccttg atgctgacga ccacctcgcc
     2641 cgcgccggcc tggggcgagg gcacgtcctc cagcaccagc gactcgggcg ggccaaactc
     2701 cttgcacagc agggctttca tgggttcctc ggttacgcgg agcgggaggc cagcccgccg
     2761 tcgatggtga cgatgcagcc ggtggtgtag gcggagcgct ccgaggcgag gaacgccacc
     2821 atccaggcga tctcctcggg cgcggccggg cgcccgaagg acatgccggc gaacagctcc
     2881 gggtagcgct ccgggtcgcc gaatttcgcc tcggcgcgct ggcggaacag gcccttcagg
     2941 cggtcggtgg ccaccggccc cgggttgacg ccgaccacgc ggatgccgtc cgcgtgcgag
     3001 gcgccgccga gggcgcgcgt gaaggccatc agcccggcat tcgccgtgct gccggcgatg
     3061 tagctcgcgt ccagtttctc gcccgcgttg cccaggacgt tgacgatcac ccccgctttc
     3121 ctcgccttca tgcgcgcgta cacctggcgc gtgaggttga ggtagccgaa catcttcagg
     3181 tcccaggcgt tgcgccaggt gtcctcgtcc acgcgcagca ggtcgccgcc ggggatcgcg
     3241 ccggcattgt tcaccaggat gtcgagatcc ccggcccagg cggcgagcgc ctcggcggcg
     3301 ccgcgctgcg acaggtcgat ggccttggcc tgcggcccgg gctgcgggcg gcgcgccgcc
     3361 accagcaccg agcagccctc ggcggcgagc acctcggcgc aggcgcggcc gatgcctttc
     3421 gaaccgccgg tcaccagcgc gcgtttgcct ttaagattca gatccatttc ccgacagtct
     3481 agcaacatgc ccctgatcac caccgacgac ggcgtgaaac tgcattacga ggaatcgggc
     3541 agcggcacgc cggtcgtgtt cgtgcacgag ttcgccggcg acctgcgcag ctgggacccg
     3601 cagctgtcgc atttctcgcg ccgctaccgc tgcatcgcct tcaacgcgcg cggctacccg
     3661 ccctccgagg tgccggagag cttcgagcgc tactcgcagg agcgcgcgcg cgacgacgtg
     3721 ctcgccgtaa tggatgcctt gaagatccag cgcgcctatg tggtgggcct gtcgatgggg
     3781 ggcttcgccg cgctgcattt cggcatcaag tacagcgcgc gcgcgctcgg cctggtggtc
     3841 gccggctgcg gctacggcgc ggagccggcg aagcgcgagg agttccagcg cgcctcgcgc
     3901 gacaacgcca aggcgctgct ggagcggggc atggccgcgt tcgccgccgg ctacggccac
     3961 aatgccgcgc ggctgcagct gcgcgcaaag gacccgcgcg gcttcgcgct gtacatcaag
     4021 tacctgaccg agcattcggc gctcggctcg gccaacacca tgctcggcta ccaggcgcgc
     4081 cggccatcgc tgtacgacct gaccgcgcag atgcagggcg tcacggcccc cacgctggtg
     4141 gtcgccggcg acgaggacga cgccacgctg gagcccggct tgctgatgaa gcgcaccatc
     4201 ccgcgctgcg ggctggtggt gctgccctgg agcggccacg cgctcaacct cgaggatccg
     4261 gcgctgttca atcgcgtcgt cgaggacttc ttccaccagg tggacaacgg gcgcttcgca
     4321 tgagcgcggg ccggctcgga gtggagcagc gcggcgcggt cggctggatc gtgttcgacc
     4381 agccgcagaa gaagaacgcc atcaacggcg ccatgtggcg cggcgttccg gaggcgatgg
     4441 cgcggttcga cgcggatgcg gccatccgct gcatcgcgtt ccgcggcgcc ggcgcggagg
     4501 ccttcgccgc tggcgcggac atttcggagt tcgagaaggt gcgcgccggc ggcggcgcgg
     4561 tgagcgagta cgacgggctg ctcgatcgcg tgctgcattc gatccaggac tcgcgcaagc
     4621 cctcggtggc gctgatccac ggcttttgca tgggcggcgg gctggaggtg gcgctcgcct
     4681 gcgacctgcg ctattgcggc gcctccgcgc agttcgccat cccggcggcc aagctcggca
     4741 tcgcctacaa cgtcgagggc cacaagcgac tgctcgagac ggtgggccac gcgcgcgccc
     4801 gcgagatcat gtttctcggc cggcgctacg gcgcagccga ggcgctcgcc atgggcctgg
     4861 tgcaccgcgt gctgccggac gcggagctcg acgcgtatgt cgacggggtg atcgccacgc
     4921 tgctggagaa cgcgccgctc gccatcgcca actccaagac catcctcgag gagtacgcca
     4981 aggcgcaagg cgccgccgac cagggccgca tgcaggctgc catcgagcgc tgcgcccgca
     5041 gcgaggacta ccaggagggg cggcgcgcct tcatggagaa gcgcaagccg cgcttcgagg
     5101 gcaaatgagc ctgcgcatca agtcggtccg cgtgcgcgcc gtcgccgcgc cgatgaagcg
     5161 gccgctcgcc accagcaccg gcgcgctgac ggtgagcccc ctgctgctcg tcgacctgaa
     5221 aaccgacgtc ggcatcgtgg ggcgctccta cctcttcggc atcggggcgc agaacctgcg
     5281 gccgatcgcg gcgctgctcg aggcgatggc cggcatgctg caaggcgacg cggtcgcgcc
     5341 gttcgacatc gaagccaagc tgcgcaagcg ctacacgctg ctcggcctgc acaacatcgt
     5401 gctgttcgcc atgtccggga tcgacatggc ggcctgggac gcgctcggcc agtcgctcga
     5461 gcagccgctg gtgcgcctgc tgggcggcgc gccccggccg gtgccggcct acaactccaa
     5521 gggcctgggg atcatgcccc tgaagcccct ggtgaaggag gcgggcgagc tcgtcgccga
     5581 gggattcggc gcggtgaagc tgcgcctcgg gcgcccggcg gccgccgacg acctcgaggc
     5641 cgtgcgcgcg gtgaagcggg cgatcgggcc gaaggtcaag ctgatggtgg acttcaacca
     5701 ggggctcacg gtggtggagg ccatcaagcg cggccgcatg atcgacgcgg agggcggcgt
     5761 gctgtggatc gaggagccgg tgcgcgcgga cgatttcgcc ggcaacgcgc gcatcgcgcg
     5821 cgaggtggcg acgccgatcc agatcggcga gaacttcatg ggcccggagc agatggcgca
     5881 ggcgctcgcc gccggggcct gcgattacgc gatgccggac gcgcagcgca tcggcggcgt
     5941 caccggctgg atgcgcgccg ccgcgctggc gcaggccgcg ggcatggaga tgtcgagcca
     6001 cctgttcccg gaggtgagct gccatctgct cgccgtgacg ccgacctgcc actggctcga
     6061 atacgtggac tgggcggacc cggtgctgga ggagccgacc cgcctgaagg acggccacgt
     6121 gctggtgccg gaacgccccg gcatcggcat gcgatggaac gaaaaggcgg tgaagcgcta
     6181 cgcgctgtga tgttcacccg ccaccacgcg cactggccgc ccggcgtgcc gaagacgctg
     6241 tcggtgccgc gcaccagcct gtactacaac ctcgaggtga gcgcgcgccg ctaccccggc
     6301 aaggcggcga tctactacta cggcaccacc ctcagctacg ccgagctgca gcgctcggcc
     6361 gaggccctgg ccggctacct gcagcgcgcc tgcggcgtgc gccggggcga ccgggtgctg
     6421 ctgtatgccc agaacagccc gcagttcgtg atcggctact acgccatcct gcgcgccgat
     6481 gccgtggtgg tgcccgtcaa tccgatgaac aagcgcgatg agctggcgta ctacgtcggc
     6541 gactccgacg cgcgcgtcgt cgtcagcacc caggacctct acggcgagat cgagccgcat
     6601 ctgggcaagg gactggcgca ctgcgtgctg gggacctatt cggactacct gcgcgaagcg
     6661 accgatctga acgtgccgga attcgtgcgc gcgccgcgcg ccgccgcagc caattggcag
     6721 gcggcgctgg ccgccgggca ccggccggac gcgcacggta ccgggccgga agacctggcg
     6781 gtgctgccct atacctcggg gaccaccggc caccccaagg gcaccatgct gacgcacgcc
     6841 aacctgctcg ccaccagcac cggcgcctgc gcctggatcc agtcctcgag cgaggcggtg
     6901 gtgctcggcg tgctg
//
LOCUS       DASRIE010000224         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1790644, whole genome shotgun sequence.
ACCESSION   DASRIE010000224 DASRIE010000000
VERSION     DASRIE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790644"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFB53_09600"
     CDS             <1..1330
                     /locus_tag="VFB53_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87023.1"
                     /translation="AYVLGAGLAGPAAGLASVAVLTLVPDASTYGLRNGFLSFHFHMV
                     VTPGASYVIGVFLASAALLGRWAPGASPRPLLASAALALGALWFRVHVFAVGFPAWLA
                     TAALATRAVRARRLLFAAGGALAFLLFVMAFYAATDSDFALPVFLDVLHDRQEPTAYD
                     GVYWALLTEHGQLLAALAGIPLVYLACLGALVVLYPLAVWLAQRARVLQATDLFPAFL
                     IVAYLAMMLTAPIDKHRDSTEFTVRPFVLVYAAVAVWTVCLLCRAFALRWPQRARQAW
                     PTLLGASLLGVALAWHGTPAMALPKFDWGWSFYPQRIDAGLTQAARYLRQHGRPGQVF
                     AMQGLELKWAATDPAVQLSSLSGMTAYLSYISAHTIEAKARERVALERYAQLARLERA
                     PSAAAALEELRRLGIAWYVVIGWNGPPWDPARRETRFLEHNVAIYAVPAS"
     gene            1354..2025
                     /locus_tag="VFB53_09605"
     CDS             1354..2025
                     /locus_tag="VFB53_09605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN87024.1"
                     /translation="MPSFWQPDWLVLREMARVLRGLLADPALELRDARVLDFGCGARP
                     YEAWFAAAGASYRGADIDAAHEVRIRDDGTLDCADGEYDLVASFQVLEHVWDLGGYLG
                     EARRALRARGWLLLSTHGSWLYHPHPHDYRRWTAEGLQREVESHGFRLVRMEPVVGPL
                     AWTTIFRSLGMAHVLKPLPLVGPPAAALAAALLNARAWLEDRITPRAITAVNACTYIA
                     LFRRA"
     gene            2028..2414
                     /locus_tag="VFB53_09610"
     CDS             2028..2414
                     /locus_tag="VFB53_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZN87025.1"
                     /translation="MVAGRFFSVGLLCALLHNAIMIGGDWLGLHYLASSLISFAIVVL
                     VGYLLHSAWTFPGAQRGAISFARYTLTMALNLPLSLAGMFVLVDLAGLGVPLAAPAVT
                     VMLALFNFLGGRWALRGRHAQSRRQT"
     gene            complement(2393..>3876)
                     /locus_tag="VFB53_09615"
     CDS             complement(2393..>3876)
                     /locus_tag="VFB53_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87026.1"
                     /translation="QDIPGAAVVIFIAIAAAWPPLARPGLALVEAMGGKPWATALITF
                     LVLCAGQLLVAKDHALAGDEHLILLQAKAFAAGRLTAQFPPELVSWVVPRPYIDLWLY
                     ASPQTGAVVSVYWPGFALLLAPFALLGIPWACNALLAAGALVLVGRLAGRLTGSPQAA
                     GWAMLLLLASPAFSGMALGYFSMTAHLFFNLLYVWVLLQRRLFLAGVVGSFALVLHNP
                     WPHFLFALPWIAWIAWHDGRRALLRLMAGYAPLTLAIGGGWWLLMRKIQGELWFAPQA
                     GDLLFYWQLQLTRVFTLPEGATLAKRTAELVKLWLWTVPGLPALMVAGWWLTRRQAPS
                     RLLGLSFAVMLAGYLFVWFDQGYGWGVRYLHPALAALPILGAAAIVTVQSEALRRYAA
                     AAAFLSLALATPFRWAQIESFMGDHLARRPPFEKDVRQIVFVTPDYPYYTQDFVQNDP
                     FLRAPVIFVLSRGPAADEKLLRERFPGARLTHEGRDGHVWRLD"
BASE COUNT          542 a         1378 c         1359 g          597 t
ORIGIN      
        1 cgcttacgtg ctcggcgcag gcctcgccgg gcccgccgcc ggtctcgcct cggtcgcggt
       61 gctgacgctg gtgcccgatg cttcgaccta tgggctgcgc aacggctttc tcagctttca
      121 cttccacatg gtcgtcaccc cgggcgccag ctatgtgatc ggcgtttttc tcgcgagcgc
      181 cgccttgctg gggcgctggg cgcccggagc ttccccgcgc ccgctgctcg cgagcgccgc
      241 gctcgcgctc ggcgcgctct ggtttcgcgt gcacgtgttc gcggtcggat ttcccgcctg
      301 gctcgccacg gcggcgctgg ccacgcgcgc ggtgcgcgcg cggcggctgc tgttcgccgc
      361 cggcggagcg ctcgccttcc tgctcttcgt gatggcgttc tatgccgcca cggattccga
      421 ttttgcgttg ccggtgttcc tcgacgtgct gcatgaccgc caggagccca ctgcctatga
      481 cggcgtctac tgggcgctcc tcaccgagca tggccagctg ctcgcggccc tcgccggcat
      541 tccgctggtg tatctggcgt gcctcggcgc gctcgtcgtc ctgtatccgc tcgcggtatg
      601 gctggcgcag cgcgcgcgag tgcttcaggc gaccgacctg tttcccgctt tcctgatcgt
      661 ggcgtacctc gcgatgatgc tgaccgcgcc gatcgacaag catcgcgatt cgaccgagtt
      721 cacggtgcgt cctttcgtcc tggtctatgc ggcggtcgcg gtctggaccg tgtgcctgct
      781 gtgccgcgcg ttcgcgctgc gttggccgca gcgcgcgcgg caggcctggc ccaccctgct
      841 cggcgccagc ctgctcggcg tggcgctcgc ctggcacggc acgccggcga tggcgctgcc
      901 taaattcgac tggggctggt cgttctaccc gcagcgcatc gacgccgggc tcacgcaggc
      961 ggcgcgctac ctgcgccagc acggccgccc cggccaggtg ttcgccatgc aggggctgga
     1021 gctgaagtgg gccgcgactg atcccgccgt tcagctcagc tcgctgagcg gcatgacggc
     1081 gtacctgtct tacatttcgg cgcacaccat cgaagccaaa gcgcgggaaa gggtcgcgct
     1141 cgagcgctat gcgcaactgg cgcggctcga gcgcgcgcct tccgcggcgg cggccctgga
     1201 ggagctgcgc cgcctcggta tcgcctggta cgtggtgatc ggctggaacg ggccgccctg
     1261 ggacccggcg cggcgcgaga cgcgcttcct ggaacacaac gtcgccatct acgcggtgcc
     1321 ggcaagctga gcatgcaacc cggccgccga gccgtgccga gcttctggca gcccgactgg
     1381 ctggtgctgc gcgagatggc gcgcgtgctg cgcggcctgc tcgccgaccc ggcgctcgag
     1441 ctgcgcgatg cgcgcgtgct ggatttcggt tgcggcgccc gaccctacga ggcctggttc
     1501 gcggccgccg gcgcaagcta ccgcggggcc gacatcgatg ccgcgcacga ggtgcgcatc
     1561 cgcgacgacg gcacgctcga ttgcgccgac ggcgagtacg acctggtggc atcgttccag
     1621 gtgctggaac atgtctggga ccttggcggc taccttggcg aggcgcgccg cgcgctgcga
     1681 gcgcgaggat ggctgctgct ctccacgcac ggcagctggc tgtaccaccc gcatccgcac
     1741 gactaccgcc gctggaccgc cgagggcctg cagcgcgagg tggaatcgca cggtttccgc
     1801 ctggtgcgca tggagccggt ggtcggcccg ctcgcctgga ccacgatctt ccgctcgctc
     1861 ggcatggccc atgttctcaa gccgctgccg ctggtcgggc cgccggcggc ggcgctggcg
     1921 gcggccctgc tcaacgcgcg ggcgtggctc gaggaccgca tcacgccgcg cgcgattacc
     1981 gcggtgaacg cctgcactta catcgcgctt ttccggcgcg cctagcgatg gtggcggggc
     2041 gtttcttctc cgtcggcctg ctgtgcgcgc tgctgcacaa cgcgatcatg atcggcggcg
     2101 actggctcgg cctgcactac ctggcctcgt ccttgatctc gttcgcgatc gtggtgctgg
     2161 tgggctacct gctgcacagc gcctggacct ttcccggtgc gcagcgcggc gcgatctcgt
     2221 tcgcgcgcta cacgctcacc atggcgctca acctaccgct gtcgctcgcc ggcatgttcg
     2281 tgctggtcga cctggcgggg ctcggcgtgc cgctcgccgc gccggcggtc accgtgatgc
     2341 tggcgctctt caacttcctc ggcggccgct gggccctgcg cggccggcac gctcagtcca
     2401 ggcgccagac gtgaccgtcg cgcccctcgt gcgtgagccg ggcgcccggg aagcgctcgc
     2461 gcaacagctt ctcgtcggcg gcgggtccgc ggctcagcac gaagatcacc ggcgcgcgca
     2521 ggaacgggtc gttctgcacg aaatcctgcg tgtaataggg gtagtccggg gtcacgaaca
     2581 cgatctggcg cacgtccttc tcgaacggcg gacggcgcgc gaggtgatca cccatgaaag
     2641 actcgatctg cgcccagcga aacggcgtgg cgagcgcgag gctgaggaag gcggcggcgg
     2701 ccgcgtagcg ccgtaaggct tcgctctgca cggtcacgat ggccgccgcg cccaggatgg
     2761 gcagggcggc gagcgccggg tgcagatagc gcacgcccca gccgtagccc tggtcgaacc
     2821 agacgaacag gtagcccgcc agcatcacgg cgaacgacag gccgagcaat cgcgacggcg
     2881 cctggcgtcg ggtgagccac cagcccgcca ccatcagcgc cggcagcccc ggcaccgtcc
     2941 acagccacag cttcaccagc tcggcggtcc gcttcgcgag cgtggcgccc tcgggcagcg
     3001 tgaacacgcg ggtgagttgg agctgccagt aaaacagcag atcgccggct tgcggggcga
     3061 accagagctc gccctggatc ttgcgcatca gcagccacca gccgccgccg atggcgagcg
     3121 tcagcggcgc gtagcccgcc atcagccgca gcagcgcacg ccggccgtcg tgccacgcga
     3181 tccaggcgat ccacggcagc gcgaacagga agtgcggcca cggattgtgc agcaccagcg
     3241 cgaacgagcc gaccacgcca gcaaggaaca ggcgccgctg cagcagcacc cagacgtaca
     3301 gcaaattgaa aaacaggtgc gccgtcatcg agaaatagcc gagcgccatg ccgctgaacg
     3361 cgggcgaggc gagcaagagg agcatggccc agcccgccgc ctgcggcgag ccggtgaggc
     3421 ggccggcgag ccggccgacc agtacgagcg cgccggccgc gagcagcgca ttgcaggccc
     3481 agggaattcc cagcagggcg aacggcgcga gcaggagcgc gaaccctggc cagtacaccg
     3541 agaccaccgc gccggtttgc ggcgaggcgt aaagccacag gtcgatatag ggacgcggca
     3601 ccacccagga gacgagctcg ggcgggaact gcgcggtcag gcggccggcg gcgaacgcct
     3661 tggcctgcag caggatcagg tgctcgtcgc cggcgagtgc atgatccttc gcgaccagca
     3721 gctgtccggc gcataggacg agaaaggtga tgagcgccgt agcccacggc ttgcccccca
     3781 tcgcctcgac cagcgcgagt cccggccgcg cgagcggggg ccacgcggcg gcaatggcga
     3841 tgaagatcac gactgcagca ccgggtatat cctgcg
//
LOCUS       DASRIE010000225         4816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1282375, whole genome shotgun sequence.
ACCESSION   DASRIE010000225 DASRIE010000000
VERSION     DASRIE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282375"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="mscL"
                     /locus_tag="VFB53_09620"
     CDS             <1..299
                     /gene="mscL"
                     /locus_tag="VFB53_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZN87027.1"
                     /translation="GVAFGKIVSSLVEDVVMPLLGSIIGAVNFAGLAVSIGSATVKYG
                     KFLQSCLDFLIIAWAIFMVVQLINRLQREKPAAPPAPPRQEVLLEEIRDLLKKG"
     gene            complement(307..2991)
                     /locus_tag="VFB53_09625"
     CDS             complement(307..2991)
                     /locus_tag="VFB53_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN87028.1"
                     /translation="MNRLLALLLLCCALPAAAADLPRKVVEIEGLAEYRLGNGLRVLL
                     VPDASADTVTVHITYLVGSRHEGYGEKGMAHLLEHLLFKGTRRHPDIKAELTRRGARF
                     NGSTSWDRTNYYETLSATADNLDWALGMEADRMANSRVSQTDLDSEMTVVRNEFEMGE
                     NNPDGVLFERMMQLAFPWHNYGNAMIGSRADIESVPIGRLQAFYRRYYRPDNALLIVG
                     GRFKAEDALSRVARHFGPLRRPKQALPALYTAEPTQDGERSVTLRRAGDAQTVAALYR
                     VPAGSHPDYPAIDVLVAALRATPQGRLHRALVQKGLASSAWGFERGLHDPGFIVFGAS
                     LGGEASLPAAREALLAVVEGIGREPLSEEDVARARLQLLNEAEKIQLDGRMLVSALSE
                     FQAGGDWRLFFLYRDRLKALTTADVNRVAAAYLKPANRVLGQFIPTAQPERAQIPATT
                     GLAAVLQAYRGAAAVATGESFDPSPQNIEARVQRRELANGVRTALLAKRTRGATVVAK
                     LVLHWGDERSTAGRTTACALAGEMLLRGTRGRSRAELRDAFDRLKTTVTVSAAGATLQ
                     TRREQLPEALRLVAEVLREPAFPPSEFEELKRSSLTSAEEQRSDPGARADEALQRHLH
                     PYPKDHWLYPQSVEERIAALQAATLEDARRCHAELVGATGADFTAVGDFDPAALTALV
                     EELFGAWKNPAPYARVQSRYFERAAIERELATPDKANAVLRAGLNLPLRDDHPDFPAM
                     VLGSYLLGGTSSARLPARVREKEGLSYSTYSWFSASSLDPVAEFQVSAIFAPQNKARV
                     EQAVREELARALGDGFSEAEVADAKRGLLEARRLARTRDDALNARLSKYLFLGRTFAW
                     DAELERRIAALAPGEVRDALRRHLDLARLAVTKAGDFR"
     gene            complement(3047..3370)
                     /locus_tag="VFB53_09630"
     CDS             complement(3047..3370)
                     /locus_tag="VFB53_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87029.1"
                     /translation="MTRRGLLLLALLAAATSPQAQSRAEAGREQFREAGRTCRYAPHA
                     EQRACMARELCKNNRNPERCEERYFVNAERRDLVLEACKGKQGRVLRDCMRDEYKKLG
                     PEPKS"
     gene            complement(3367..3876)
                     /locus_tag="VFB53_09635"
     CDS             complement(3367..3876)
                     /locus_tag="VFB53_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN87030.1"
                     /translation="MTIKVGDRVPEGKLLEATEFDSATQCALPPKPVEIAGAVKGKRI
                     AVFGLPGAYTPTCSAKHVPGYLANIDKLRAKGVDEVWCVAVNDAFVMAHWGRDQKALG
                     RIRFLGDGSALWTKALGLEIDRSAAGMGVRMQRFSMLVDDGVVKQLNIEAPGKFEVSD
                     AETMLKQLG"
     gene            3953..4374
                     /locus_tag="VFB53_09640"
                     /pseudo
     CDS             3953..4374
                     /locus_tag="VFB53_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            complement(4381..>4816)
                     /locus_tag="VFB53_09645"
     CDS             complement(4381..>4816)
                     /locus_tag="VFB53_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN87031.1"
                     /translation="LTAAVGAFGFGYLQDAIGHVRAMALTIVGWIVMIATAYFADDAP
                     TFWVAANLAGLCMGSAQAAGRAIVGYLAPRTREAEFFGLWGLAVKAASIFGPLTYGAV
                     TWIFAGNHRLGILAVGVYFVIGLALLKSIDVERGRRAALQSA"
BASE COUNT          685 a         1669 c         1725 g          737 t
ORIGIN      
        1 tcggcgtagc cttcggcaag atcgtgtcct cgctggtgga agacgtggtc atgccgctgc
       61 tcggctcgat catcggcgcg gtgaacttcg ccggcctcgc ggtgagcatc ggctcggcca
      121 ccgtgaagta cggcaagttc ctgcagtcct gcctggattt cctcatcatc gcctgggcga
      181 tcttcatggt ggtgcagctg atcaaccgcc tgcagcgcga aaagcctgct gcgccgccgg
      241 cgccgccgcg gcaggaagtg ctgctcgagg agatccgcga cctgctgaag aagggctgag
      301 cgccgcctat ctaaaatcgc ccgccttggt caccgccagg cgtgcgaggt cgaggtggcg
      361 gcgcagcgcg tcgcgcacct cgcccggggc gagggcggcg atgcgccgct ccagttcggc
      421 atcccaggcg aaagtgcgcc ccaggaacag gtatttggac agccgcgcgt tgagcgcgtc
      481 gtcgcgcgtg cgcgccagcc gccgcgcctc gagcagtccg cgcttggcat ccgccacttc
      541 cgcctcgctg aagccatcgc ccagggcgcg cgcgagctcc tcgcgcaccg cctgctccac
      601 gcgcgccttg ttctgcggcg cgaagatcgc cgacacctgg aattcggcga ccgggtcgag
      661 cgagctggcg ctgaaccagc tgtaggtgct gtaggacagc ccttccttct cgcgcacgcg
      721 cgccggcagg cgggcgctcg aggtgccgcc cagcaggtag ctgccgagca ccatcgcggg
      781 gaagtccgga tggtcgtcgc gcaggggcag gttgagccct gcgcgcagca cggcgttcgc
      841 cttgtccggg gtggccagct cccgctcgat cgcggcgcgc tcgaagtagc ggctctgcac
      901 gcgcgcgtag ggggcgggat tcttccaggc gccgaacagt tcctcgacca gggcggtgag
      961 cgccgccggg tcgaagtcgc cgaccgccgt gaaatccgcg ccggtggcgc cgaccagctc
     1021 ggcgtggcag cgccgggcgt cctccagcgt ggcggcctgc agcgccgcaa tgcgctcctc
     1081 gaccgactgc gggtacagcc agtggtcctt cgggtagggg tgcaggtggc gctgcagcgc
     1141 ctcgtcggcg cgcgcgccgg ggtcgctgcg ctgctcctcg gcgctggtga gcgaactgcg
     1201 cttgagttcc tcgaattcgc tcggcgggaa ggccggctcg cgcagcacct cggccaccag
     1261 gcgcagcgcc tctggcagct gctcgcgccg tgtctgcagg gtggcgccgg cggcgctgac
     1321 cgtgaccgtg gtcttcagcc ggtcgaaggc gtcgcgcaac tcggcgcgcg aacgcccgcg
     1381 cgtgccgcgc agcagcatct ccccggcgag cgcgcaggcg gtggtgcgcc cggcggtgct
     1441 gcgctcgtct ccccaatgca ggaccagctt ggccaccacc gtcgcgccgc gcgtgcgctt
     1501 ggcgagcagc gcggtgcgca cgccgttggc caattcgcgg cgctgcacgc gggcctcgat
     1561 gttctgcggc gacgggtcga agctctcccc ggtcgccacc gccgcggcgc ccctgtaggc
     1621 ctgcagcacg gccgccaggc cggtggtggc gggaatctgc gcgcgctcgg gctgcgcggt
     1681 ggggatgaac tgcccgagga cgcggttggc gggcttcagg taggcggctg ccacgcggtt
     1741 cacgtcggcc gtggtgagcg ccttcaggcg gtcgcggtac aggaagaaca gccgccagtc
     1801 gccgcccgcc tggaactccg acagcgcgct caccagcatg cgcccgtcga gctggatctt
     1861 ctcggcttcg ttcagcaatt gcagcctggc gcgcgccacg tcctcctcgc tcaagggctc
     1921 gcggccgatg ccctcgacca ccgccagcag cgcctcgcgc gccgccggca gggatgcctc
     1981 gccgcccagg ctggcgccga acacgatgaa gcccggatcg tgcaggccgc gctcgaagcc
     2041 ccaggcggaa ctggcgaggc ccttttgcac cagggcgcgg tgcagccgcc cctgcggcgt
     2101 cgcgcgcagc gccgccacca gcacgtcgat cgccgggtag tccgggtggc tgcccgccgg
     2161 cacccggtac agggccgcca cggtctgcgc gtcgccggcg cgccgcagcg tcaccgagcg
     2221 ctcgccgtcc tgcgtgggct cggcggtata gagcgccggc agcgcctgct tggggcggcg
     2281 cagcgggccg aaatgcctgg ccacgcgcga cagggcgtcc tccgccttga agcgcccgcc
     2341 gacgatgagc agcgcgttgt cgggccggta gtaccggcgg tagaaggcct gcagcctgcc
     2401 gatcggcacc gactcgatgt cggcgcgcga gccgatcatg gcgttgccgt agttgtgcca
     2461 ggggaaggcg agctgcatca tgcgctcgaa cagcacgccg tcgggattgt tctcgcccat
     2521 ctcgaactcg ttgcgcacca ccgtcatctc gctgtcgagg tcggtctggc tgacgcgcga
     2581 gttcgccatg cggtcggctt ccatgccgag cgcccagtcg aggttgtccg cggtcgccga
     2641 cagcgtctcg tagtagttgg tgcggtccca ggaggtgctg ccgttgaagc gcgccccgcg
     2701 gcgcgtcaac tcggccttga tgtcgggatg gcgccgcgtg cccttgaaca gcaggtgctc
     2761 gagcaggtgc gccatgccct tctcgccgta gccctcgtgg cgcgagccga cgaggtaggt
     2821 gatgtgcacg gtgacggtgt cggcgctcgc gtcgggcacc agcaggacgc gcaggccgtt
     2881 gccgaggcgg tactcggcga ggccctcgat ctcgaccacc ttgcgcggca ggtcggcggc
     2941 ggccgccggc agggcgcagc acagcaggag gagggcgagg agtctgttca tcggcaggcg
     3001 ggccaggctg cttggagccg ggccgcgggc aaaggttcgg cgcgcgctag gacttcggct
     3061 cggggcccag cttcttgtat tcgtcgcgca tgcagtcgcg cagcacgcgg ccctgcttgc
     3121 ccttgcaggc ctcgagcacc aggtcgcgcc gctcggcatt gacgaaatag cgttcctcgc
     3181 agcgctccgg gttgcggttg ttcttgcaca gctcgcgcgc catgcaggcg cgctgctcgg
     3241 cgtgcggcgc gtagcggcag gtacggccgg cctcgcggaa ctgctcgcgg ccggcctcgg
     3301 cgcgcgactg cgcctgcggg gaggtggcgg cggcgaggag ggcgaggagc agcagtccgc
     3361 gccgcgtcat cccagttgct tgagcatcgt ttcggcgtcg ctcacctcga acttgccggg
     3421 ggcctcgatg ttgagctgct tcaccacgcc atcatccacc agcatcgaga agcgctgcat
     3481 gcgcacgccc atgccggcgg cgctgcgatc gatctccagg ccgagcgcct tggtccagag
     3541 cgcgctgccg tcgccgagga agcggatcct gcccagcgcc ttctgatcgc ggccccaatg
     3601 ggccatgacg aaagcatcgt tcaccgccac gcaccagacc tcgtccacgc ccttcgccct
     3661 caacttgtcg atattggcca ggtagcccgg aacgtgtttg gcggagcagg tcggcgtgta
     3721 ggcgcccggc aggccgaaga cggcgatgcg ctttcccttg acggcccctg cgatttcgac
     3781 gggcttgggc ggcagcgcgc actgcgttgc ggaatcgaac tccgttgcct ccaggagctt
     3841 gccctcgggc acccggtcgc cgaccttgat cgtcatgttg gcttgccttt aagtgaagtt
     3901 gcggggagcg gggataataa cgcgccagca ttccaatcta aaggagactc gcatgcagcg
     3961 caaaggttcg gcgatatgga aaggcggcct gaaggacggc aatggccagg tatccacggg
     4021 cagcgggggc cttgccgcgg cgccttatac ctttgccagc cgcttcgagg ccggcggcaa
     4081 gacggatccc gaggagctga tcgccgcggc gcacgcggcg tgcttctcga tggcgctctc
     4141 cggccagctc ggccaggcca acctcaggcg gagagcatcg cgacgacggc caatctcacc
     4201 ttggaaaaga ccgacgccgg gatgacggtg accgcgatcc acctggtggt caaggcgaag
     4261 gtcccgggag ccgaccgggc cgccttcgac aaggcagccg gcaacgccaa ggcggggtgt
     4321 ccgatatcgc ggctcctcaa cgccaagatc acgatggaat cggcgctgga gtagtcctgt
     4381 tcaggcgctt tgcagcgccg cgcgccggcc gcgctcgacg tcgatgctct tcagcagcgc
     4441 cagcccgatg acgaaataca cccccaccgc gaggatgccc aggcggtggt tgccggcgaa
     4501 gatccaggtg accgcgccgt aggtgagggg gccgaagatc gaagccgcct tgaccgccag
     4561 cccccacagg ccgaagaact ccgcttcgcg cgtgcgcggc gcgaggtagc cgacgatggc
     4621 gcgacccgcg gcctgcgccg atcccatgca cagcccggcc aggttggcgg ccacccagaa
     4681 ggtcggcgcg tcgtcggcga agtaggcggt ggcgatcatc acaatccagc cgacgatggt
     4741 cagcgccatg gcgcgcacgt ggccgatggc gtcctgcagg tagccgaagc cgaaggcgcc
     4801 gactgcggcc gtgagg
//
LOCUS       DASRIE010000226        13103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2048541, whole genome shotgun sequence.
ACCESSION   DASRIE010000226 DASRIE010000000
VERSION     DASRIE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048541"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFB53_09650"
     CDS             <1..192
                     /locus_tag="VFB53_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN87032.1"
                     /translation="PEDTLMFLCRSGARSHAAAATATQAGWRESYNVLEGFEGDKDAS
                     EHRNTVGGWRKAGLPWVQG"
     gene            complement(189..983)
                     /locus_tag="VFB53_09655"
     CDS             complement(189..983)
                     /locus_tag="VFB53_09655"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZN87033.1"
                     /translation="MDASLFHALILGIVEGLTEFLPVSSTGHLILAGDLLGYRGARAG
                     VFFVAIQTGAMLAVVWEYRARFFAVDPALWRNLLVAFIPAAALGLLFGGFIKSHLFGP
                     VPVALAFIAGGLVILLVERGGRIARVASTGAMTWLDALKVGVAQCFALIPGTSRSGAT
                     IIGGMLFGLSRPAATEFSFFLAVPTLVAAGAYDLWKNRGLFSADDLGMFGVGLAASFA
                     SAFVVIRWLVRYVATHDFRPFAWYRIAFGIVVLATAYGGLVNWSAP"
     gene            complement(1000..1536)
                     /locus_tag="VFB53_09660"
     CDS             complement(1000..1536)
                     /locus_tag="VFB53_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN87034.1"
                     /translation="MLEEAGAARGFSWLLLDYRGYGQSAGAPSEAALVADAFALYDYA
                     LGLPGADPKRIYAFGRSLGSGVAVALAAQRPLAGLILATPYDSLAAVAKRYYWYLPVD
                     WLLKHRFDSVARAPQIKAPLLCLIAERDEVIPAAHAERLFEAWGGAKRRVVLAGAGHN
                     TSDAHPMFWPAIREFLEK"
     gene            complement(1791..3671)
                     /gene="thiC"
                     /locus_tag="VFB53_09665"
     CDS             complement(1791..3671)
                     /gene="thiC"
                     /locus_tag="VFB53_09665"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZN87035.1"
                     /translation="MNANPKFLAATAHVDDAAVKPLPNSTKVYVQGSRPDLRVPMRRV
                     SQADTPTMFGGEANPPVFVYDCSGPYTDPAAGIDIRSGLAPLRQPWIEERGDTERLAG
                     PSSQYGIERLNDARLAELRFDLQRSPRRAKAGRNVSQMHYARKGIVTPEMEFIAIREN
                     LQREAYIESLKATGKTGEKMLELLMRQHRGESFGASIPGSITPEFVRSEVARGRAIIP
                     ANINHPESEPMIIGRNFLVKINANIGNSAVSSGIGEEVEKMTWAIRWGGDTVMDLSTG
                     KHIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEELTWEIYRDTLIEQAEQGVDYFT
                     IHAGVLLRYVPMTAKRMTGIVSRGGSIMAKWCLAHHRESFLYTHFEEICEIMQAYDVS
                     FSLGDGLRPGSGYDANDEAQLSELRTLGELTAIAWKHDVQVMIEGPGHVPMQLIKENM
                     DIQLKECHEAPFYTLGPLTTDIAPGYDHITSAIGAAQIGWYGTAMLCYVTPKEHLGLP
                     NKKDVKDGIMAYKIAAHAADLAKGHPGAQIRDNAVSKARFEFRWEDQFNLGLDPDTAK
                     DFHDETLPKDSMKVAHFCSMCGPHFCSMKITQDVRDYVEKGMQEKSVEFVKKGAALYH
                     KT"
     regulatory      complement(3661..3759)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3834..4481
                     /locus_tag="VFB53_09670"
     CDS             3834..4481
                     /locus_tag="VFB53_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN87036.1"
                     /translation="MSTPANAKNEPRWTRRKEARPEEITAAALELFIERGFAATRLED
                     IAARAGISKGTLYLYFANKEDLFKAVVREGLVSPIAEMRGLVEQFEGDSFELLRLLIH
                     GWWEKVGATALAGIPKLVLGEAGNFPELVRFYLAEVVEPGLAVMTAIVKRGMARGEFR
                     AVNAEEVALLIGAPLLQMMLWRCALEPQSTRKMIPERYLDAHLDMLRHGLVATAE"
     gene            4486..5619
                     /locus_tag="VFB53_09675"
     CDS             4486..5619
                     /locus_tag="VFB53_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN87037.1"
                     /translation="MSKSRLIMFAAVVVAGIAAAGFAAVRGKHAGQAPAAAAPIAEFL
                     QNDLYIVEPIALERALPLTGTLTPLTEATVKAKVAGELVAVAVREGESVQQGQMLARI
                     DLTEVQAKVAAREAEVQAAKAQLVWAEKNRNQQKALLDKAFISQSAFDSIQSNYDVAA
                     AKLRAAAAELVVARKSQGDAVLTAPFSGIVAQRHAQPGERVALDAKVISIVDLSRLQL
                     EASVPPAAIGLVKVGLPMNFRVEGFGEREFAGRIERINPSATAGSRSISVYAAIDNRE
                     GLLRGGMFAQGALTLSRIDNALAVPASAVREEIGQTFVYAIDDGMLKRKNVKVGPADA
                     AGRVQVLEGLAAGDRIVRVNLGALREGVAARLSGPQPATAAKQ"
     gene            5626..8751
                     /locus_tag="VFB53_09680"
     CDS             5626..8751
                     /locus_tag="VFB53_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZN87038.1"
                     /translation="MWFTRVSIANPVFATMMMAALLVLGAFSYSRLPVEQFPDVSFPV
                     VVVQTEYPGASPENVEEEVTRRVEESVNTISGIKTLSSRSYEGLSVVIIEFDLSIDPK
                     LAAQDVREKIALIRSGFRDEIKEPRVSRFNPDDFPIVSVAVQSSGRSLRELTTLADQV
                     IKKRLENVGGVGQATLVGGVKREIKVYLNLEAMEAQRIGADQVVQALRSENQEIPAGS
                     ILTPSQEKIVQLRARLTGPQDFRNLIVARRGGRAVTLGAIAEIEDGQQEEDNAALVNG
                     QRALSIDIIKAQGENTIAVVDGVRRVTRELQGFLPSDVKIDIVRDASTGIRNSVADVK
                     TTLLEGGLLTIAIVFLFLASWRSTVITGLTLPIALIGTFLFLYAFGFTLNVLTLMALS
                     LSVGLLIDDAIVVRENIVRHVALGADHHTAALEGTREIGLAVLATTLSIVAVFLPVGF
                     MGGIIGRFFHQFGLTVVSAVLISMFVSFTLDPMLSSIWRDPQAEGKFSSGPLGRLLAW
                     FHGLMERISVAYERLLGWSLAHRKSVLAIAFASFLASFPLMQLVGSEFVPEPDLSELQ
                     VQFKTPVGASLELTSQKARQAEAALREFAEVKYTYATVNTGFVQGKNRVNIFVQLLPR
                     KERARSQNELTRPMRERLARIAGIELQQIGAYKSVSSGKPLQVSILGQERKVLAEIAE
                     QVMAAVREVPGAVDAESSDEAAKPQFAVELKRELASDLGIGVGQVAATLRPLLAGQAV
                     GNWRGPDDQYYDVQVRLSRADRARSEDLQRVPLASSQLDAGGAPKMVSLREIANIVPG
                     SGPQQINRKEQLREVLITANVYNRAAGDAAAELRAKLDRIAMPPGYRYTMGGSSKDIQ
                     ESLYFASQALALAVIFIYLILASQFGSFIQPVAIMMSLPLSLIGVVLALILFRSTLNI
                     FSIIGFIMLMGLVTKNAILLIDFVNHERRGGKARREAVLAAGRIRLRPILMTTLAMIF
                     GMIPLSLGLGEGGEQRAPMGQAVIGGVITSGLLTLIVVPVIYTYLDDLAAWLRRFGKL
                     PAPPQSAPRTP"
     gene            8748..9401
                     /locus_tag="VFB53_09685"
     CDS             8748..9401
                     /locus_tag="VFB53_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZN87039.1"
                     /translation="MNFEQARSNMVEQQIRTWEVLDQSVLDLLYAVPREDFVPAACRT
                     LAFADLEIPLGEGETMMAPKMEARILQELALRKTDRVLEVGTGSGYLTALLARRAAQV
                     HSLEIRPALAAFGRANLERHGADNVTLAIGDGARGDPKRAPYDAIVLTGSTPVLPKSL
                     LDQLAPGARLFAVVGEPPVMVARLVSCTAPGAYRSAQLFDTLLAPLLNAERASRFRF"
     gene            9414..10736
                     /locus_tag="VFB53_09690"
     CDS             9414..10736
                     /locus_tag="VFB53_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZN87040.1"
                     /translation="MRRSFALALLACAPLAAAQNLAQVFQDARGYDAQFGAARYALQA
                     GLERVPQGRALLLPSLNLTANTTRANTESESKNPAAQPSFERDIDSHGYALTFTQPLY
                     RRQNWLQVDQAEFQAKQAEAQFGQAGQDLILRTAQAYFDVLAAQDTLALVRAQKVAIS
                     EQLAQAKRNFEVGTATITDTHEAQARFDLAAAQEISALNDVDARHRALQQLTGKEYAA
                     LKPLRPDVKLAPPNPADMQSWVDLAEKQSYAVLVQEAGTEIAALEAKRNRAGHAPTLD
                     FVASHGYTSRSASELSTTGSEITTNSISLQFALPLYQGGAISSREREAAFNMEKSRQD
                     LENARRGAALATRQSYLAVINGIAQVGALEQALVSSQSALESNRLGYEVGVRINIDVL
                     NAQQQVFSTRRDLAVARYNTITNFLRLKAAAGGLREEDLEEVNRALQP"
     gene            complement(10714..11973)
                     /locus_tag="VFB53_09695"
     CDS             complement(10714..11973)
                     /locus_tag="VFB53_09695"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HZN87041.1"
                     /translation="MRTIYTVLLHAALPWILARLWWRGRREPGYREAIAERFGRSELV
                     PKAKLLWLHAVSVGEVRACAPLVRALQRENPDHDVLITCLTAAGRDTAKQVYGESVLL
                     AYLPYDTPWAVRRFLEHYRPRLGVLVETEIWPNLMRACAELGVPVMLANARMSRKSVQ
                     AYQRLRWLSRPAFANLGAVCAQGPADARRLRRLGAANVSIAGNLKFDIEPDADKAAEG
                     KSFRAALRHRKVLLLASTREGEEEQLLGALGAQDDGVLIMLVPRHQRRFDEVAALAAR
                     MGFTLARRSLGEAPHQGRRLYLGDTMGEMAFYFAMCDVAVIGGSFEPLGGQNLIEASA
                     AGVPVVTGPHMFNFADVTRLAVEAEAALQAPDAAAAVRAARSLLEDPERCARMGIAGK
                     RLCAQHRGAAERHLAACRALLRAAGPG"
     gene            11993..12961
                     /locus_tag="VFB53_09700"
     CDS             11993..12961
                     /locus_tag="VFB53_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN87042.1"
                     /translation="MRVLLTGCAGFIGMHCALRLLARGDEVLGADSLSPYTSVELKRD
                     RLRRLEAQRGFRFEQTELAEAAACARLFDAWRPELVLHLAAQPGVRYALQDPASYVQS
                     NLVAFANLLEACRQRAPRHLVYASSSSVYGANAKLPWCEADNVDHPISLYAATKKANE
                     LMAHVASHLHGLPASGLRYFTVYGPWGRPDMSPMLFARAIMEGKPIQVFNHGDMRRDF
                     TYVDDAVESTLRVLDAAPTAKPAHRVLNVGNRQPVALLEYIGMLEEAFGRKAEKQMLP
                     MQPGDVPATYADTRALEALTGFSPATPLKEGLARFADWFRSYYRHP"
BASE COUNT         2077 a         4594 c         4467 g         1965 t
ORIGIN      
        1 cccgaggaca ccctgatgtt cctttgccgc tcgggcgctc gctcgcacgc cgccgccgcc
       61 accgccacgc aggccggctg gcgcgagagc tacaacgtgc tggaaggttt cgagggcgac
      121 aaggacgcca gcgagcaccg caataccgtc ggcggctggc gcaaggcggg gctgccatgg
      181 gtgcagggtt agggcgcgct ccagttcacc aggccgccgt aggcggtcgc cagcaccacg
      241 atcccgaagg caatccggta ccaggcgaac ggccggaagt cgtgcgtcgc cacgtagcgc
      301 accagccagc ggatgacgac gaacgccgag gcgaaggacg ccgccagccc gacgccgaac
      361 atgccgaggt cgtccgcgga gaacagcccg cggttcttcc acaggtcgta ggcgccggct
      421 gcgacgagcg tcggcaccgc gaggaagaag gagaattcgg tcgccgccgg ccgcgacaac
      481 ccgaacagca tgccaccgat gatggtggcg cccgagcgtg aggtgccggg aatcagcgcg
      541 aagcactgcg cgacgcccac cttcagcgca tccagccagg tcatcgcccc ggtgctcgcg
      601 acgcgggcga tgcgcccgcc gcgctcgact agcaggatca ccaagccgcc ggcgatgaag
      661 gcgagcgcca ccggcaccgg tccgaagaga tggctcttga tgaagccgcc gaacagcagg
      721 ccgagcgcgg cggccgggat gaacgccacc aggagattgc gccagagcgc cggatccacg
      781 gcgaaaaagc gcgcgcggta ttcccacacc accgccagca tcgcgccggt ctggatcgcg
      841 acgaagaaca cgccggcgcg cgcgccgcgg tagccgagca ggtcgccggc caggatcagg
      901 tgtcccgtgc tcgacaccgg caggaattcc gtcagtccct cgacgatgcc caggatcaac
      961 gcgtggaaaa gggaggcgtc catgaaaagg ggtctgaccc tatttttcta gaaattcgcg
     1021 gatcgcgggc cagaacatgg ggtgcgcgtc gctcgtgttg tgtcccgccc cagcgaggac
     1081 caccctgcgc ttggcgccgc cccaggcctc gaacaggcgc tcggcgtgcg ccgcggggat
     1141 cacctcgtcg cgctcggcga tgaggcacag cagcggcgcc ttgatctgcg gcgcgcgcgc
     1201 gacggaatcg aagcggtgct tcagcaacca gtccacgggc aggtaccagt agtagcgctt
     1261 ggcgaccgcg gcaaggctgt cgtagggcgt ggcgagaatc agtccggcga gcgggcgctg
     1321 tgccgcaagc gccacggcga cgccgctgcc caggctgcgg ccgaaagcgt agatgcgctt
     1381 cgggtccgcg ccgggcagcc cgagcgcgta gtcgtacaag gcgaaggcgt cggcgaccag
     1441 cgccgcctcg gatggcgcgc ccgcgctctg cccgtagccg cggtagtcca gcagcagcca
     1501 cgagaacccg cgcgccgcgc ccgcctcctc gagcatccac gacacctctt ccgcgttgcc
     1561 gccgaaatac agcacgaggg gcgcgcccga cttgaggtgc caggcgtgga accggatgcc
     1621 atccgttccc tgcaggaaca cgctggcgac cgccgggtgc cgccgcgcga tctccgcgcg
     1681 ccggccctcg gcgatcggct ggcgctggaa gatcagggcc tcctgcgcga ggtagacgac
     1741 gaggggcagc ccgaccgcga cggcaatccc gaccttgagg atcgagccga tcaggtcttg
     1801 tggtatagcg cggcgccctt cttcacgaac tccaccgact tctcctgcat gcccttttcg
     1861 acgtagtcgc gcacgtcctg cgtgatcttc atcgagcaga aatgcggccc gcacatcgag
     1921 cagaaatgcg ccaccttcat cgagtccttg ggcagcgtct cgtcgtggaa gtccttggcg
     1981 gtgtccgggt cgagacccag gttgaactgg tcttcccagc gaaattcgaa gcgcgccttg
     2041 gaaacggcgt tgtcgcggat ctgcgcgccc ggatggccct tggcgaggtc ggcggcgtgg
     2101 gcggcgatct tgtaggccat gatgccgtcc ttcacgtcct tcttgttcgg caggccgagg
     2161 tgctccttcg gcgtgacgta gcacagcatc gccgtgccgt accagccgat ctgcgccgcg
     2221 ccgatggcgc tggtgatgtg gtcgtagccc ggtgcgatgt cggtggtgag cggccccagc
     2281 gtgtagaagg gtgcctcgtg gcattccttc agctggatgt ccatgttctc cttgatgagc
     2341 tgcatcggga cgtgcccggg gccttcgatc atgacctgga cgtcgtgctt ccaggcaatg
     2401 gccgtcaatt cacccaaggt tctcagttcg gaaagctgcg cctcgtcgtt ggcgtcgtag
     2461 cccgaaccgg gccggagtcc atcgccgagc gagaagctga cgtcgtaggc ctgcatgatt
     2521 tcgcagatct cctcgaagtg cgtgtagagg aagctctccc tgtggtgcgc gaggcaccac
     2581 ttcgccatga tcgaaccgcc gcgcgacacg atgccggtca tgcgcttcgc cgtcatcggc
     2641 acgtagcgca gcagcacccc ggcgtggatg gtgaagtagt ccacgccctg ctcggcctgt
     2701 tcgatcagcg tatcgcggta gatctcccag gtgagctcct cggccttgcc gtcgactttc
     2761 tcgagggcct ggtagatcgg cacggtgccg atcggcaccg gagaattgcg gatgatccac
     2821 tcgcgcgtct cgtggatgtg cttgccggtg gagaggtcca tcaccgtgtc cccgccccag
     2881 cggatggccc aggtcatctt ctcgacctcc tcgccgatgc ccgaggacac cgccgagttg
     2941 ccgatgttgg cattgatctt caccaggaaa ttgcggccga tgatcatcgg ctccgattcc
     3001 gggtggttga tgttggccgg gatgatggcg cggccgcgcg ccacctcgct gcgcacgaac
     3061 tcgggcgtga tgctgccggg gatcgaggca ccgaaactct cgccgcggtg ctgccgcatc
     3121 aggagctcga gcatcttctc gcccgtcttg ccggtggcct tcaggctctc gatgtacgcc
     3181 tcgcgctgca ggttttcgcg aatcgcgatg aattccatct ccggcgtgac gatccccttt
     3241 ctcgcgtaat gcatctgcga gacgttcctg cccgcctttg cgcgccgcgg cgaacgctgc
     3301 aggtcgaagc gcagctcggc gagcctcgca tcgttcaggc gctcgatgcc gtactgcgag
     3361 ctcggcccgg cgaggcgctc ggtgtcgccg cgctcctcga tccacggctg ccgcagcggc
     3421 gcgaggccgg agcggatgtc gatgccggcc gccgggtcgg tgtagggccc cgagcagtcg
     3481 tagacgaaca ccggcgggtt ggcttcgccg ccgaacatcg tcggcgtgtc ggcctgcgac
     3541 acccggcgca tcggcacccg gaggtccggg cgcgagccct gcacgtagac cttcgtcgaa
     3601 ttgggcagcg gcttgaccgc cgcgtcatcg acgtgggcgg tggcggcgag aaacttggga
     3661 ttggcgttca tgggcgcttc cttcggcggc attacccgca tcaggttatg agggactctc
     3721 tcacccgccc cgaccgggac aggacccctg cgtgcccaat agtttgccgc gcttgacctt
     3781 tttgcagtgc cgcattatat tactgaccgt tcagtcactt tcccggtgtc cccttgtcca
     3841 cacccgccaa cgccaagaac gagccccgct ggacgcgccg caaggaagcg cgccccgagg
     3901 agatcaccgc cgccgcgctc gagctgttca tcgagcgcgg cttcgccgcc acgcgcctcg
     3961 aagacatcgc cgcgcgcgcc ggcatctcca agggaacgct gtacctgtat ttcgccaaca
     4021 aggaagacct gttcaaggcc gtggtgcgcg aagggctggt gtcgccgatc gccgagatgc
     4081 gcggcctggt ggagcagttc gaaggcgaca gcttcgagct gctgcgcctg ctgatccacg
     4141 gctggtggga aaaggtcggc gcgacggcgc tggcgggcat ccccaagctg gtgctcggcg
     4201 aggcggggaa cttccccgag ctggtgcggt tctacctggc cgaggtggtc gagcccggcc
     4261 tcgccgtgat gaccgcgatc gtgaagcgcg gcatggcgcg cggcgaattc cgtgcggtga
     4321 acgccgaaga ggtggcgctg ctgatcggcg cgccgctgct gcagatgatg ctgtggcgct
     4381 gcgccctcga gccgcaatcg acgcgcaaga tgattcccga gcgctacctg gacgcgcacc
     4441 tggacatgct gcggcatggc ctggtcgcaa ctgcggagta gggccatgag caagagcaga
     4501 ctgatcatgt tcgcggccgt cgtggtcgcc ggcatcgcgg ccgccggctt cgccgccgtg
     4561 cgcggcaagc acgcggggca ggcgcccgcc gcggccgcgc ccatcgccga gttcctgcag
     4621 aacgacctct acatcgtcga gccgatcgcc ctggaacgcg cgctgccgct cactggcacg
     4681 ctcacgccgc tgaccgaagc gaccgtcaag gccaaggtgg cgggcgagct cgtcgccgtc
     4741 gcggtgcgcg aaggcgaaag cgtgcagcag ggccagatgc tggcgcgcat cgacctcacc
     4801 gaggtgcagg ccaaggtcgc cgcgcgcgaa gccgaggtgc aggccgcgaa ggcgcagctg
     4861 gtgtgggccg agaagaaccg caaccagcag aaggcgctgc tcgacaaggc gttcatctcg
     4921 cagagcgcgt tcgacagcat ccagagcaat tacgacgtcg cagcggccaa gctccgcgcc
     4981 gccgccgccg agctggtggt ggcgcgcaag tcgcagggcg acgcggtgct gaccgcgccg
     5041 ttctccggca tcgtggcgca gcgccacgcc cagcccggcg agcgcgtcgc gctggacgcc
     5101 aaggtgatca gcatcgtcga cctgtcgcgc ctgcagctgg aggcctcggt gccgccggcg
     5161 gcgatcggcc tggtgaaagt cggcctgccg atgaatttcc gcgtcgaggg attcggcgag
     5221 cgcgaattcg ccggccgcat cgagcgcatc aacccgtcgg ccaccgccgg ctcgcgctcg
     5281 atcagcgtat acgcggcgat cgacaaccgc gagggcctgc tgcgcggggg catgttcgcg
     5341 caaggcgcgc tgacgttgtc gcgcatcgac aatgccctcg ccgtgccggc gtccgcggtg
     5401 cgcgaggaaa tcggccagac cttcgtctac gccatcgacg acggcatgct gaagcgcaag
     5461 aacgtcaagg tcgggccggc cgacgccgcc ggacgcgtgc aggtgctcga gggcctcgct
     5521 gcgggcgacc gcatcgtgcg cgtcaatctc ggcgcgctgc gcgagggcgt cgccgcccgg
     5581 ttgtcgggcc cgcagcccgc caccgccgcg aagcagtagc cccccatgtg gttcaccagg
     5641 gtcagcatcg ccaatccggt attcgcgacc atgatgatgg ccgcgctgct ggtgctcggc
     5701 gcgttctcct attcgcgcct gccggtggag cagttcccgg acgtgagctt cccggtggtg
     5761 gtggtgcaga ccgagtaccc cggggcctcg ccggaaaacg tcgaggagga agtgacccgg
     5821 cgcgtggaag agtcggtcaa caccatcagc ggcatcaaga cgctttcctc gcgctcctac
     5881 gaggggctgt cggtggtgat catcgagttc gacctgtcga tcgaccccaa gctcgcggcg
     5941 caggacgtgc gcgagaagat cgcgctgatc cgctcggggt tccgcgacga gatcaaggag
     6001 ccgcgcgtga gccggttcaa cccggacgat ttccccatcg tctcggtggc agtgcagtcc
     6061 agcggccgca gcctgcgcga actgaccacg ctcgccgacc aggtgatcaa gaagcggctg
     6121 gagaacgtgg gcggcgtcgg ccaggcgacg ctggtgggcg gggtgaagcg cgagatcaag
     6181 gtgtatctca acctggaggc gatggaggcg cagaggatcg gcgccgacca ggtggtgcag
     6241 gcgctgcgct cggagaacca ggagatcccg gccggatcga tcctcacgcc cagccaggag
     6301 aagatcgtgc agctgcgcgc gcgcctcacc gggccgcagg acttccgcaa cctgatcgtg
     6361 gcgcggcgcg gcggcagggc ggtgacgctg ggggcgatcg ccgagatcga agacggccag
     6421 caggaagagg acaacgccgc gctggtaaac ggccaacgcg cgctgtcgat cgacatcatc
     6481 aaggcgcagg gcgagaacac catcgcggtg gtcgacggcg tgcgacgcgt gacgcgcgaa
     6541 ctgcagggct tcctgccctc ggacgtgaag atcgacatcg tgcgcgacgc ctccaccggc
     6601 atccgcaatt cggtcgccga cgtgaagacc acgctgctcg agggcgggct gctcaccatc
     6661 gccatcgtgt tcctgttcct cgcctcgtgg cgcagcacgg tgatcaccgg gctcaccctg
     6721 ccgatcgcgc tcatcgggac tttcctgttc ctctacgcgt tcggcttcac gctcaacgtg
     6781 ctcacgctga tggcgctgtc gctgtcggtg ggcctcctga tcgacgacgc catcgtggtg
     6841 cgcgagaaca tcgtgcgcca cgtggcgctg ggcgccgacc accacaccgc ggcgctggag
     6901 ggcacgcgcg agatcggcct ggcggtgctc gccaccacgc tgtcgatcgt cgccgtgttc
     6961 ctgccggtgg gcttcatggg cggcatcatc gggcgctttt tccaccagtt cggcctgacg
     7021 gtggtatcgg cggtgctgat ctcgatgttc gtgtccttca ccctcgaccc gatgctgtcc
     7081 tcgatctggc gcgatccgca ggccgagggc aagttcagct cgggcccgct cgggcggttg
     7141 ctggcctggt tccacggcct gatggagcgc atttcggtgg cctacgagcg cctgctcggc
     7201 tggagcctcg cgcaccgcaa gagcgtgctg gccatcgcct tcgccagctt cctcgccagc
     7261 ttcccgctga tgcagctcgt cggctcggaa ttcgtccccg agcccgacct gtcggagctg
     7321 caggtgcagt tcaagactcc ggtcggcgct tcgctcgaac tcacttcgca gaaggcgcgc
     7381 caggcggagg cggcgctgcg cgagtttgcg gaagtgaagt acacctacgc caccgtcaat
     7441 accggcttcg tgcagggcaa gaaccgcgtc aacatcttcg tccagctgct gccgcgcaag
     7501 gagcgcgcgc gctcgcagaa cgagctcacg cggccgatgc gcgagcgact ggcgcgcatc
     7561 gccggcatcg agctgcagca gatcggcgcc tacaagtcgg tgtccagcgg caagccgctg
     7621 caggtgtcga tcctcggcca ggagcgcaag gtgctcgccg agatcgccga gcaggtaatg
     7681 gccgcggtgc gcgaagtgcc cggcgccgtg gacgccgaat cgagcgacga ggcggccaag
     7741 ccccagttcg ccgtcgagtt gaagcgggaa ctggcgagcg acctcggcat cggcgtcggc
     7801 caggtcgccg cgacgctgcg gccgctgctc gccggccagg cggtcggcaa ctggcgcggt
     7861 cccgacgacc agtactacga cgtgcaggtg cgcttgtcgc gtgccgaccg cgcgcgcagc
     7921 gaggacctgc agcgcgtgcc gctcgcctcc agccagctgg atgccggcgg cgcgccgaag
     7981 atggtcagcc tgcgggagat cgccaacatc gttccgggca gcgggccgca gcagatcaac
     8041 cgcaaggagc agctgcgcga ggtgctgatc accgccaacg tctacaaccg ggccgccggc
     8101 gacgccgcgg ccgagctgcg cgcgaagctg gaccgcatcg ccatgccgcc gggctaccgc
     8161 tacaccatgg gcggctcgag caaggacatc caggagtcgc tgtatttcgc ctcgcaggcg
     8221 ctggcgctgg cggtgatctt catctacctg atcctcgctt cgcagttcgg cagcttcatc
     8281 cagccggtgg cgatcatgat gtcgctgccg ctgtcgctga tcggcgtggt gctggcgctg
     8341 atcctgttcc gctccacgct caacatcttt tccatcatcg gcttcatcat gctgatgggc
     8401 ctggtcacga agaacgcgat cctgctgatc gatttcgtga accacgaacg ccgcggcggc
     8461 aaggcgcgcc gcgaggcggt gctggcggcc gggcgcatcc gcctgcgccc gatcctgatg
     8521 accaccctgg cgatgatctt cggcatgatc ccgctctccc tcggcctggg cgagggcggc
     8581 gagcagcgcg cgccgatggg ccaggcagtg atcggcgggg tgatcacctc cggcctgctc
     8641 acgctgatcg tcgtgccggt gatctacacc tacctcgacg acctggcggc gtggctgagg
     8701 cgctttggta aactacccgc cccgccgcaa tccgccccca ggacgccgtg aacttcgagc
     8761 aagcgcgcag caacatggtc gagcagcaga tccgcacctg ggaggtgctc gaccagtcgg
     8821 tgctcgacct gctgtacgcg gtgccgcgcg aggatttcgt gccggccgcc tgccgcacgc
     8881 tcgccttcgc cgacctggag attcccctcg gcgagggcga gacgatgatg gcgccgaaga
     8941 tggaggcgcg catcctgcag gagctggcgc tgcgcaagac cgaccgcgtg ctcgaagtcg
     9001 gcaccggcag cggctacctc accgcgctgc tcgcacgccg cgcggcgcag gtgcattcgc
     9061 tcgagatccg cccggcgctg gccgccttcg gccgcgccaa cctggagcgc cacggcgccg
     9121 acaacgtcac gctcgcgatc ggcgacggcg cgcgcggcga cccgaagcgc gcgccctacg
     9181 acgccatcgt gctgacgggt tcgactccgg tcttgccgaa atccctgctt gaccagctcg
     9241 cgcccggcgc gcgcctgttc gccgtggtcg gcgagccgcc ggtgatggtg gcgcgcctgg
     9301 tgagctgcac ggcgcccggc gcctaccgca gcgcccagtt gttcgatacc ctgctcgccc
     9361 cgctcctcaa cgcggaacgc gcgtcgcgct tccgcttctg aaggcatgca ccgatgagac
     9421 gcagcttcgc cctcgccctg ctcgcctgcg cgccgctcgc cgccgcccag aacctggccc
     9481 aggtgttcca ggacgcgcgc ggctacgatg cgcagttcgg cgcggcgcgc tacgcgctgc
     9541 aggcgggcct ggagcgcgtg ccgcaggggc gcgcgctgct gctgcccagc ctcaacctga
     9601 cggcgaacac cacgcgcgcg aacaccgagt cggaatcgaa gaaccccgcg gcgcaaccga
     9661 gcttcgagcg cgacatcgat tcgcacggct acgccctcac cttcacgcag ccgctgtacc
     9721 gccggcagaa ctggctgcag gtcgaccagg ccgagttcca ggccaagcag gccgaggccc
     9781 agttcgggca ggccgggcag gacctgatcc tgcgcacggc acaggcctat ttcgacgtgc
     9841 tggcggcgca ggacacgctc gcgctggtgc gcgcgcagaa ggtcgccatc tccgagcagc
     9901 tggcgcaggc aaagcgcaac ttcgaggtcg gcaccgcgac catcaccgac acgcatgagg
     9961 cgcaggcgcg cttcgacctc gccgcggcgc aggagatctc ggcgctgaac gacgtggacg
    10021 cgcggcaccg cgccctgcag cagctcaccg gcaaggaata cgccgcgctc aagccgctgc
    10081 ggccggacgt caagctcgcc ccgcccaacc ccgcggacat gcaaagctgg gtcgacctgg
    10141 ccgagaagca gtcctacgcg gtgctggtgc aggaggcggg caccgagatc gcggccctgg
    10201 aagccaagcg caaccgcgcc gggcacgcgc cgacgctcga tttcgtcgcc agccacggct
    10261 atacctcgcg ctcggcgtcg gagctgagca ccaccggcag cgagatcacc accaattcga
    10321 tcagcctgca gttcgcgctg ccgctgtacc agggcggcgc catcagctcg cgcgagcgcg
    10381 aggcggcgtt caacatggag aagtcgcgcc aggacctgga gaacgcgcgc cgcggcgcgg
    10441 cgcttgccac gcgccagtcc tacctcgccg tgatcaacgg catcgcgcag gtgggcgcgc
    10501 tcgagcaggc gctggtgtcc tcgcagagcg cgctggaatc caaccgcctg ggctacgaag
    10561 tgggcgtgcg catcaacatc gacgtgctga acgcgcagca gcaggtgttc tccacgcggc
    10621 gcgacctggc ggtggcgcgc tacaacacca tcaccaactt cctgcgcctg aaggccgccg
    10681 ccggcggctt gcgcgaagag gacctcgaag aggtcaaccg ggccctgcag ccctgagcag
    10741 ggcgcggcag gccgcgaggt ggcgctcggc cgccccgcgg tgctgcgcgc acaggcgctt
    10801 gccggcgatg cccatgcgcg cgcagcgttc cgggtcctcc agcagggagc gcgccgcgcg
    10861 caccgccgcc gcggcatccg gcgcctgcag cgccgcctcg gcctccaccg ccagacgcgt
    10921 cacgtccgcg aaattgaaca tgtgcggccc ggtgaccacc ggcacgccgg cagccgaggc
    10981 ctcgatcagg ttctgcccgc cgagcggctc gaagctgcca ccgatcaccg ccacgtcgca
    11041 cattgcgaag tagaaggcca tctcccccat ggtgtctccc aggtacaggc gccggccctg
    11101 gtgcggcgcc tcgcccaggc tgcggcgcgc cagcgtgaaa cccatgcgcg cggcgagcgc
    11161 cgcgacctcg tcgaaccggc gctggtgccg cggcaccagc atgatcagca cgccgtcgtc
    11221 ctgggcgccc agcgcgccga gcagctgctc ctcctcgccc tcgcgcgtgc tggcgagcag
    11281 cagcaccttg cgatgccgca gggcggcgcg aaacgacttg ccctcggccg ccttgtccgc
    11341 atcgggctcg atgtcgaact tgaggttgcc ggcgatggac acgttcgccg cgcccaggcg
    11401 gcgcagacgg cgcgcatccg ccgggccctg cgcgcacacc gcgcccaggt tggcgaaggc
    11461 cgggcgcgac agccagcgca ggcgctggta agcctgcacg gattttctcg acatgcgcgc
    11521 attggcgagc atcaccggca cgcccagttc cgcgcaggcg cgcatcaggt tgggccagat
    11581 ttcggtctcc accagcacgc cgagccgcgg ccggtaatgc tcgaggaagc gccgcaccgc
    11641 ccagggagtg tcgtaaggca ggtaggcgag cagcaccgat tcgccgtaca cctgtttcgc
    11701 ggtgtcgcgc ccggccgcgg tcaggcaagt gatgagcaca tcgtggtccg gattctcgcg
    11761 ctgcagcgcg cgcaccagcg gcgcacaggc gcgcacttcg ccgacggaca ccgcgtgcag
    11821 ccagagcagc ttcgccttgg gcacgagctc gctgcggccg aaacgctccg cgatcgcctc
    11881 ccggtaaccc ggctcgcgcc ggccgcgcca ccacaggcgc gcgaggatcc agggcagcgc
    11941 cgcgtgcagc aggacggtat agatcgtgcg caaggtgtcc tatgataaga ccatgcgcgt
    12001 actactcacc ggctgcgcag gattcatcgg catgcactgc gcgctgcgcc tgctcgcgcg
    12061 cggcgacgag gtgctcggcg ccgacagcct ttcgccctac accagcgtcg agctcaaacg
    12121 cgaccgcctg cgccgcctgg aggcgcagcg cggctttcgc ttcgagcaga ccgagctcgc
    12181 cgaggcggcg gcctgcgcgc gactattcga cgcctggcgg cccgagctgg tgctgcacct
    12241 cgcggcgcag cccggcgttc gctacgcgct gcaggatccc gcctcctacg tccagtcgaa
    12301 cctcgtcgcc ttcgccaacc tgctcgaagc ctgccgccag cgcgcgccgc gccacctggt
    12361 gtacgcctcg agctccagcg tgtacggcgc caacgcgaag ctgccctggt gcgaagccga
    12421 caacgtggac catccgatca gcctctacgc ggcgaccaag aaggcgaacg aactgatggc
    12481 gcacgtggcg agccacctgc acggcctgcc cgccagcggc ctgcgctact tcaccgtcta
    12541 cggcccctgg ggccgcccgg acatgtcgcc gatgctcttc gcgcgcgcca tcatggaggg
    12601 caagccgatc caggtgttca accacggcga catgcggcgc gacttcacct acgtcgacga
    12661 tgcggtggaa agcaccttgc gcgtgctgga tgcggcgccc acggcgaagc cggcgcaccg
    12721 ggttctcaac gtcggcaacc gccagccggt ggcgctgctg gagtacatcg ggatgctgga
    12781 ggaagccttc ggccgcaagg cggaaaagca gatgctgccg atgcagccgg gcgacgtgcc
    12841 ggcgacctac gcggacacgc gcgcgctcga agcgctgacc ggcttctcgc cggcgacgcc
    12901 gctcaaggag ggcctggcgc gcttcgccga ctggttcagg agctactacc gccacccgtg
    12961 acgccgccgc ccgcgggcgc gtattccggc acccaggcgc gcaattggcg gcgcacggcc
    13021 tcggcgcccg gatcgcgccc cgccgcgatc cacgccagca cctcctcgag cagctcggcg
    13081 ccggcgctcg attgcgcgcg cgc
//
LOCUS       DASRIE010000227         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1000956, whole genome shotgun sequence.
ACCESSION   DASRIE010000227 DASRIE010000000
VERSION     DASRIE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000956"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1432)
                     /locus_tag="VFB53_09705"
     CDS             complement(11..1432)
                     /locus_tag="VFB53_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HZN87043.1"
                     /translation="MDIRRLLVSTALYGIADVLVMAVGGFLLLPLYTRTLAQGEFGMY
                     VAIRSNVEILTYLLYFGLPSAVARVYFDHRQLQRQGEYLSSIVNFFVIVLAGSCGVLW
                     LWGPSLWNALSPTTPAHPYLPYAVALAAVSFPAAIASLWLRMESRPLAMVAMQLGASA
                     VLAALAAFNLLVLDAGLPGLLVALLASAAVPAAALAWLFGRSYRPVIRRAHVTESLRY
                     ALPILLGYISYFVLNRLSTLILQRHVPIEDLAVYGLAQQLSMIVALAGTSFGMALQPA
                     VFGAQPADALDIIQRTGRVLLVLMFGVTSALMLFADDLFAFVAPSSYGDGHTVLLLLV
                     VANFTNAFTLMSDTALLYHRRPKTSVAISMVGAVASAALGFWLIPRYQLHGAALSVVG
                     AFVIRMLISHWMAWRVTGYAYVGPMLGAMAAVSALAWATGWIGFAALPEIAPAGVKAG
                     LGALILLTTYYLYRRLHSTPCVK"
     gene            complement(1582..2691)
                     /gene="gmd"
                     /locus_tag="VFB53_09710"
     CDS             complement(1582..2691)
                     /gene="gmd"
                     /locus_tag="VFB53_09710"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZN87044.1"
                     /translation="MNKVALITGITGQDGAHLAEFLLDKGYVVHGLRRRVSLFNTDRI
                     DHLYQDPHEPDTRLFLHYGDMTDSSSLVRIIQQVQPDEIYNLAAQSHVHVSFEEPEYT
                     ANSDALGPLRILEAVRLLGLTKKTRFYQASTSEMFGAVREVPQTEATPFYPRSPYGVA
                     KLYAHWITINYREAYGIYACNGILFNHESAIRGETFVTRKITRGLARIKLGLQKCLYL
                     GNLDARRDWGHARDYVEAQWLMLQQDEPEDFVIATGVQYSVRDFVSAAARELGMALRW
                     EGHGVDEKGWWIDPSAPAGARERLVVTVDPRYFRPAEVETLLGDAAKAKRKLGWSPRT
                     SFEELVREMVREDLQHAERDALVNRHGYRTMDAHE"
     gene            complement(2711..3436)
                     /locus_tag="VFB53_09715"
     CDS             complement(2711..3436)
                     /locus_tag="VFB53_09715"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HZN87045.1"
                     /translation="MRERGALLGVVPARGGSKRLPRKNVRPLAGRPLIAWTVDAARAS
                     GVLDDVLVSTDDAEIAEAARAAGASVPWLRPAELATDTAASVDVLRHALAWHEAERGP
                     VEAVVLLQPTSPFRSAASIRGAVAAFRAQAGGEPQAVASVSRALQHPAWCFRIDGEAM
                     QPFLGWEPLRQRSQDLPPAYALNGAVYVVPAAALRRGAPLLGPGLRAFVMDDPREALD
                     IDDADDWRAAESLAPQLAGERPH"
BASE COUNT          532 a         1221 c         1294 g          471 t
ORIGIN      
        1 attcgggtga tcatttgacg catggcgtgc tgtgcaatct gcggtacagg tagtaggtgg
       61 tcaacaggat gagcgcgccc aggcccgcct tgacgccggc cggggcgatc tcgggcaggg
      121 cggcgaagcc gatccagccc gtcgcccagg cgagcgcgga caccgcggcc atggcgccca
      181 gcatcgggcc cacgtacgcg tagccggtga cgcgccaggc catccagtgg ctgatcagca
      241 tgcggatgac gaacgcgccg accaccgaca gcgccgcgcc gtgcagctgg tagcgcggga
      301 tgagccagaa gccgagcgcc gccgaggcga cggcgccgac catcgagatc gccaccgacg
      361 tcttcgggcg gcggtggtag agcagggcgg tgtccgacat cagcgtgaac gcgttggtga
      421 agttcgccac caccagcagc agcagcaccg tgtgcccgtc gccgtacgag ctgggcgcga
      481 cgaacgcgaa caggtcgtcg gcgaacagca tcagcgcgct cgtcacgccg aacatcagca
      541 ccagcaggac gcggccggtg cgctggatga tgtcgagcgc gtccgccggc tgggcgccga
      601 acaccgccgg ctgcagcgcc atgccgaacg acgtgccggc cagcgccacg atcatcgaga
      661 gctgctgggc gaggccgtac acggcgaggt cctcgatcgg cacgtgccgc tgcaggatca
      721 gcgtgctgag gcggttcagc acgaagtagg agatgtagcc cagcaggatg ggcagcgcgt
      781 agcgcagcga ctccgtcacg tgcgcgcggc ggatgaccgg ccggtacgag cgcccgaaga
      841 gccacgccag cgcggcggcc ggcaccgccg cgctggccag cagcgccacc agcaggccgg
      901 gcaggccggc gtcgagcacc agcaggttga aggcggccag cgccgcgagc accgccgacg
      961 cgccgagctg catcgccacc atggcgagcg gccggctctc catgcgcagc cacagcgacg
     1021 cgatggcggc cgggaagctc accgcggcga gcgcgacagc gtacggcagg taggggtgcg
     1081 ccggcgtggt gggcgacagc gcgttccaca gcgacggccc ccacagccac agcacgccgc
     1141 aggagccggc gagcacgatc acgaagaagt tcacgatcga gctgaggtac tcgccctggc
     1201 gctgcagctg ccggtggtcg aagtagacgc gcgccacggc cgacggcagc ccgaagtaca
     1261 ggaggtacgt gaggatctcg acgttcgagc ggatggcgac gtacatcccg aactcgccct
     1321 gcgcgagcgt gcgcgtgtac agcggcagca gcaggaagcc gcccaccgcc atgacgagca
     1381 cgtcggcgat gccgtacagc gccgtcgaga cgaggagtcg ccggatgtcc actcaggtcc
     1441 ttcgcggtcg cgcgcggcgc cccggagcct gtgaacgatt cgcctgcgaa cggtcggctc
     1501 ggccctgacc gagccgactc cgcggcacag cgtcgcaggg cctgtcctca gccagtcgaa
     1561 gggtgcggtc gcgccgtcgg ctcactcgtg cgcgtccatc gtgcggtagc cgtggcggtt
     1621 caccagcgcg tcgcgctcgg cgtgctgcag gtcctcgcgc accatctcgc gcacgagctc
     1681 ctcgaagctc gtgcgcggcg accagccgag cttgcgcttg gccttggcgg cgtcgccgag
     1741 cagcgtttcc acctcggccg ggcggaaata gcgcgggtcc accgtgacca ccagccgctc
     1801 gcgcgcgccg gccggcgcgc tcgggtcgat ccaccagccc ttctcgtcga cgccgtggcc
     1861 ttcccagcgc agcgccatgc cgagctcgcg cgccgcggcg ctcacgaagt cgcgcacgct
     1921 gtactgcacg ccggtggcga tgacgaagtc ctccggctcg tcctgctgca gcatgagcca
     1981 ctgcgcctcg acgtagtcgc gcgcatggcc ccagtcgcgg cgggcgtcga ggttgccgag
     2041 gtacaggcac ttctgcaggc cgagcttgat gcgcgccagg ccgcgcgtga tcttgcgggt
     2101 gacgaaggtc tcgccgcgga tcgccgactc gtggttgaac aggatgccat tgcaggcgta
     2161 gatgccgtac gcctcgcggt agttgatggt gatccagtgc gcgtagagct tggccacgcc
     2221 gtacgggctg cgcggataga agggcgtggc ctcggtctgc ggcacctcgc gcaccgcgcc
     2281 gaacatctcc gaggtgctcg cctggtagaa gcgcgtcttc ttcgtgaggc cgagcagccg
     2341 caccgcctcg aggatgcgca gcggcccgag cgcgtcggag ttggcggtgt actcgggctc
     2401 ctcgaagctc acgtgcacgt gcgactgcgc ggcgaggttg tagatctcgt cgggctgcac
     2461 ctgctggatg atgcgcacca ggctcgagct gtcggtcatg tcgccgtagt gcaggaacag
     2521 gcgcgtgtcc ggctcgtgcg ggtcctggta caggtggtcg atgcggtcgg tgttgaacag
     2581 cgacacgcgc cggcgcaggc cgtggacgac gtagcccttg tcgagcagga actcggcgag
     2641 gtgggcgccg tcctggcccg tgatgccggt gatgagtgcg accttgttca tgtgtcgagt
     2701 cttcggtttt tcaatggggg cgctcgcccg cgagctgcgg ggccagcgat tcggcggcgc
     2761 gccagtcgtc ggcgtcgtcg atgtccaggg cttcgcgcgg gtcgtccatc acgaaggcgc
     2821 gcagtccggg gccgagcagc ggcgcgccgc ggcgcagcgc ggcggccggc acgacgtaca
     2881 ccgcgccgtt gagcgcatag gccggcggca ggtcctgcga gcgctggcgc agcggctccc
     2941 agccgaggaa cggctgcatc gcctcgccgt cgatccggaa gcaccaggcg ggatgctgca
     3001 gcgcgcggct gacgctcgcc acggcctgcg gctcgccgcc ggcctgcgcg cgaaacgccg
     3061 ccaccgcgcc gcggatcgag gccgcgctgc ggaacgggct ggtgggctgc agcagcacca
     3121 ccgcctcgac gggcccgcgc tcggcctcgt gccaggcgag cgcatggcgc agcacgtcga
     3181 cgctcgcggc ggtgtcggtg gcgagctcgg cggggcgcag ccacggcacc gacgcgcccg
     3241 ccgcgcgcgc cgcctcggcg atctcggcgt cgtcggtgga cacgagcacg tcgtcgagca
     3301 cgccgctggc gcgcgcggcg tcgacggtcc acgcgatcag cggccgcccc gcgagcggcc
     3361 gcacgttctt gcgcggcagc cgcttgctgc cgccgcgcgc aggcaccacg ccgagcaggg
     3421 cgccgcgctc acgcatggaa gatgcccccg atgtcgaact gcgcgcgctg gaagtcctcc
     3481 atgcggccga tgtcgagcca gtactcgtgc atcgggaa
//
LOCUS       DASRIE010000228         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1288315, whole genome shotgun sequence.
ACCESSION   DASRIE010000228 DASRIE010000000
VERSION     DASRIE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288315"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFB53_09720"
     CDS             complement(<1..542)
                     /locus_tag="VFB53_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZN87046.1"
                     /translation="MPSKREQRSLFGEILDWMLAPLLLLWPMSVVLTWLVAQGIANKP
                     YDRQLGEMARVLSRQVTVQRGSDTPVARFALPAGTSEILHTDETDDIHYQVLGLRGEF
                     LSGEPHLPVPPDEERAPPGELRFRDDVLNNDSVRVAYLWVEVPGSDSGPAPLVQVAET
                     LGKRSRLATEIIKGVILPQFV"
     gene            complement(579..1250)
                     /locus_tag="VFB53_09725"
     CDS             complement(579..1250)
                     /locus_tag="VFB53_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN87047.1"
                     /translation="MRILIAEDDQVLADGLLRSLRNAGYAVDQVSSGSEADAALAAHE
                     FDLVILDLGLPKIHGLEVLRKLRARGSSVPVLILTAADSVEQRVKGLDLGADDYMAKP
                     FSLQELEARVRALTRRGLGTASTVIKHGPLTFDATGRVAYINDQMIELSARELSLLEV
                     LLQRSGRLVSKDQLVERLCEWGEEVSNNAIEVYIHRLRKKIEQGPVRIATVRGLGYCL
                     EKIPG"
     gene            1423..2526
                     /gene="recA"
                     /locus_tag="VFB53_09730"
     CDS             1423..2526
                     /gene="recA"
                     /locus_tag="VFB53_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZN87048.1"
                     /translation="MDAPVKALNTEKAKALQAALAQIEKQFGKGSIMRLGEGEVIEDI
                     EVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQRMGGTCAFIDA
                     EHALDVQYAQKLGVNLQELLISQPDTGEQALEIVDALVRSGSVDLVVIDSVAALTPKA
                     EIEGEMGDALPGLQARLMSQALRKLTGTIKKTNTMVVFINQIRMKIGVMFGSPETTTG
                     GNALKFYASVRLDIRRIGSIKRGEEVIGNETKVKVVKNKVSPPFKTAEFDILYGEGIS
                     REGEIIDMGVEAKVLEKSGAWYAYQGEKIGQGKDNAREFLRENPDLAVEVENKVREAL
                     GIPLRAVGESAPAGGAEAAAPEPRPARGKAAKE"
     gene            2561..3136
                     /locus_tag="VFB53_09735"
     CDS             2561..3136
                     /locus_tag="VFB53_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZN87049.1"
                     /translation="MPTRPGRRSAAQAHAPLSLKARAIALLAQREHSEAELRRKLLRH
                     ARSLVDRQAGEGAPGEAAPGEAAANDPALRVDPVIDWLRAEGHLSETRFVESRVHARA
                     ARFGNLRIRQELAQHGVELPPEAAQALKASELERATEVWRRKFGAAPVDAAQRARQSR
                     FLAQRGFSADVIARVMRDAGARIGDEPASDP"
     gene            3395..>3553
                     /locus_tag="VFB53_09740"
     CDS             3395..>3553
                     /locus_tag="VFB53_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN87050.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGYPAFSVHEATEAAQKLGGPVWV
                     VKAQIHAGG"
BASE COUNT          557 a         1225 c         1274 g          497 t
ORIGIN      
        1 acgaactgcg gcaggatcac gcccttgatg atctcggtcg ccaggcgcga gcgcttgccg
       61 agcgtctcgg ccacctgcac cagcggcgcc ggcccggagt cgctgcccgg cacctccacc
      121 cacaggtagg cgacgcgcac gctgtcgttg ttgagcacgt cgtcgcggaa gcgcagctcg
      181 ccgggcggcg cgcgctcctc gtcaggcggc accggcagat ggggctcgcc gctcaggaac
      241 tcgccgcgca ggccgagcac ctggtagtgg atgtcgtcgg tctcgtccgt gtgcaggatc
      301 tcggaggtgc cggccggcag cgcgaagcgc gcgacgggcg tgtcgctgcc gcgctgcacc
      361 gtgacctggc gcgacagcac gcgcgccatc tcgcccagct gccggtcgta gggcttgttc
      421 gcgatgccct gcgccacgag ccaggtgagc acgacgctca tcggccacag cagcagcagc
      481 ggggcgagca tccagtcgag gatctcgccg aagagggaac gctgttcgcg cttgctgggc
      541 atcggcggcg agcatagccg cgcccgccgc gcgcgctcct accccgggat cttctccagg
      601 cagtatccca gcccgcgcac cgtggcgatg cgcaccggcc cctgctcgat cttcttgcgc
      661 aggcggtgga tgtacacctc gatggcgttg ttgctcacct cctcgcccca ctcgcagagc
      721 cgctcgacca gctggtcctt gctgactagg cggcccgagc gctgcagcag cacctccagc
      781 aggctcagct cgcgcgcgga gagctcgatc atctggtcgt tgatgtaggc cacgcggccg
      841 gtggcgtcga aggtgagcgg cccgtgcttg atcacggtgg aggccgtgcc gaggccgcgg
      901 cgcgtgaggg cgcgcacgcg cgcctccagc tcctgcagcg agaacggctt ggccatgtag
      961 tcgtcggcgc ccaggtcgag ccccttgacg cgctgctcga cgctgtcggc ggcggtcagg
     1021 atgagcacgg gcacggacga gccgcgcgcg cgcagcttgc gcagcacctc gaggccgtgg
     1081 atcttcggca ggccgaggtc gaggatgacc aggtcgaact cgtgggccgc gagcgcggcg
     1141 tcggcctcgc tgccgctgct cacctggtcg acggcatagc cggcgttgcg cagcgagcgc
     1201 agcaggccgt cggccagcac ttggtcatct tcggcaatca ggatgcgcat cgcttgtctc
     1261 cggtgcgatg aatgttcttg ggcggcagcg gccgccgtgg ggcgattcta ggttttgcgg
     1321 cgaacccccc cggtccggtg caccgcgagg ggtgcacccg ccgcatcact gtacggatat
     1381 ccagcttttg aatatgatcc gccgaacttc tggaggacat cgatggacgc accggtcaag
     1441 gccctgaaca ccgagaaagc caaggcgttg caggcggccc tggcgcagat cgagaaacag
     1501 ttcggcaagg gctcgatcat gcggctcggc gagggcgagg tcatcgagga catcgaggtc
     1561 gtctccaccg gctcgctcgg gctggacatc gcgctcggcg tcggcggcct gccgcgcggg
     1621 cgggtggtcg agatctacgg ccccgagtcc agcggcaaga ccaccctcac gctgcaggtg
     1681 gtggccgaaa tgcagcgcat gggcggcacc tgcgccttca tcgacgccga gcacgcactc
     1741 gacgtgcagt acgcgcagaa gctgggcgtc aatctgcagg agctgctgat ctcgcagccg
     1801 gacacgggcg agcaggccct cgagatcgtc gatgcgctgg tgcgctccgg ctcggtcgac
     1861 ctggtggtga tcgactccgt cgccgcgctc acgccgaagg ccgagatcga aggcgagatg
     1921 ggcgatgcgc tgcccggcct gcaggcgcgc ctcatgagcc aggcgctgcg caagctcacc
     1981 ggcaccatca agaagaccaa caccatggtc gtcttcatca accagatccg catgaagatc
     2041 ggcgtgatgt tcggctcgcc ggagaccacc accggcggca acgcgctcaa gttctatgcc
     2101 tcggtgcgcc tggacatccg ccgcatcggc agcatcaagc ggggcgagga ggtcatcggc
     2161 aacgaaacca aggtgaaggt ggtgaagaac aaggtctcgc cgccgttcaa gaccgccgag
     2221 ttcgacatcc tctacggcga aggcatcagc cgcgaaggcg agatcatcga catgggggtc
     2281 gaggccaagg tgctcgagaa gagcggcgcc tggtacgcct accagggcga gaaaatcggc
     2341 cagggcaagg acaacgcgcg cgagttcctg cgcgagaacc ccgatcttgc ggtcgaggtc
     2401 gagaacaagg tgcgcgaggc gctgggcatc ccgctgcggg cggtcggcga gtccgcgccg
     2461 gcaggcggcg ccgaggcggc tgcgcccgag ccgcgcccgg cgcgcggcaa ggcggccaag
     2521 gagtaggggt gcggcgcgcc cgcgtcgcca ccgctgcgcc atgcccaccc gtcccggccg
     2581 gcgctcggcg gcgcaggcgc acgcgccgct gtcgctgaag gcgcgggcca tcgccctgct
     2641 cgcgcagcgc gagcacagcg aggccgagct gcggcgcaag ctgctgcgcc acgcgcggtc
     2701 gctggtcgat cggcaagccg gcgaaggcgc gcccggcgaa gccgcgcccg gcgaagccgc
     2761 ggcgaacgac ccagcgcttc gggtcgaccc cgtcatcgat tggctgcgcg ccgaggggca
     2821 cctgagcgaa acgcgcttcg tcgagagccg ggtgcacgcg cgcgccgcgc gtttcggcaa
     2881 cctgcgcatc cgccaggaac tcgcgcagca cggcgtcgag ctgccgcccg aggcggcgca
     2941 ggccttgaag gcgagcgagc tcgagcgcgc caccgaagtc tggcgccgca agttcggcgc
     3001 tgcgccggtc gatgcggcgc agcgcgcgcg ccagtcgcgc ttcctcgcgc agcgcgggtt
     3061 ctcggccgac gtgatcgcgc gggtcatgcg cgacgcgggc gcacgcatcg gcgacgagcc
     3121 cgcctcggac ccgtgaacga ggggcacgcc caccgccctg acgcacgtca gggcggtggc
     3181 gagcgccgtg ggccggctgt cagtttgcgt gcagacctgc gtgctacatt gcgccgcgct
     3241 gccggaaaag gccgtgcccg tcggggtgcg catggggcct gtcgccggca tgggtgcccc
     3301 ccgggcccca cggtggtacc gcacacccgc cgcagacccc tcccgctgag gccttccggc
     3361 cgccgctttt cgaaaactcg tcagagagtc cgctatgaag atccatgagt atcaaggcaa
     3421 ggaaatcctg cgccagttcg gcgtgccggt gccgcgcggc tatcccgcgt tcagcgtgca
     3481 cgaagccacc gaagcggcgc aaaagctggg cggcccggtc tgggtggtga aggcgcagat
     3541 ccatgccggc ggt
//
LOCUS       DASRIE010000229         6032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3340948, whole genome shotgun sequence.
ACCESSION   DASRIE010000229 DASRIE010000000
VERSION     DASRIE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3340948"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFB53_09745"
     CDS             complement(<1..363)
                     /locus_tag="VFB53_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZN87051.1"
                     /translation="MEDSYNYKVVRQFALATIVWGVVGMAVGVLIAAQLLWPELNFGV
                     PWLSYGRLRPLHTNAVIFAFGGCALFASSYYVVQRTSHARLFCDKLAAFTFWGWQAVI
                     VLAAITLPMGITSSKEYAE"
     gene            complement(405..1526)
                     /locus_tag="VFB53_09750"
     CDS             complement(405..1526)
                     /locus_tag="VFB53_09750"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZN87052.1"
                     /translation="MAVFAAGFRPFFLLASAWAALAVPVWLAAYVHGYALRGTLPALF
                     WHAHEMVFGFGFAAVAGFLLTAIPNWTGRLPVRGAALAALAALWFVGRVGLLISGHVG
                     APVAAVLDLAFPVALIAVVARELVAGRNWRNLPMLAALSLLLAGNVLVHLHALGVAYS
                     AMLGIRVGIATLLMLIALIGGRIVPSFTRNWLAKARPGVPPPAPEGVLDRAALLVTLA
                     ALAAWVFAVAEWPLAVAGLALTARLARWRGKATLAEPLLFVLHLGYGWLALGLVFLGV
                     DGSSAALHALTVGAIGTMTLAVMTRASLGHSGRPLVADGATRTIYALVSAAAVLRVLS
                     AFSGAHLALVTSLSGLAWSAAFATFAVHYGRFLLQPRLT"
     gene            1596..3380
                     /locus_tag="VFB53_09755"
     CDS             1596..3380
                     /locus_tag="VFB53_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN87053.1"
                     /translation="MPGRRISFATIAVSGLGAFVALAVGATLYVSASRGLRSTHAWIA
                     QQAEARLDALEQRLEARLRPVADQGESIAEAFADGRIDPARPAVLDAFMFGALGATPQ
                     VSDLGIVDPFGRTRRWSRGALAASSADWSQRPEVRKWLVAGREQDGPAWRPLVWEDAA
                     VRGPSLLHQVALQRAGNFVGMLGQVVPMAKLSEDLAVFGAEYGVTPFVLYGPRADLVV
                     AHRAFAGAGTASGGLPAIVDAGDPVLARLHSPDGPALLSGALKRAKGVRASVSETTYV
                     FLTREVQLHGREPWTIGLYFDPVQGGQRAEMMRTLYSIGVGLAVLALAVALAAIAGRR
                     LGRPIEALARAAHLVRKGRLGEVQTLPRSRIYELDEANRSFAEMVEGLRERNVMRETL
                     GQFLPEQVALELLAGGGRIEPEEAKATVLIGDIEGFATLTDTLGAHRTIEFLNAYFEV
                     MVGIIERYRGVVTQFQGDAVLAVFNVPIADRDHGANALRAALEIVRAAQAQSFAGVRA
                     LNRVGISTGRVVAGAVGAAGRLTYTVHGNAVNLAARLEALNKEYGTRILLSDKTAERC
                     PGFRLRKVADAQIRGYGAPVALYTPEER"
     gene            complement(3387..4334)
                     /locus_tag="VFB53_09760"
     CDS             complement(3387..4334)
                     /locus_tag="VFB53_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZN87054.1"
                     /translation="MRLVGPRQPLRCETTDVPMPGPGEVLIKVRACGVCRTDLHLADG
                     ELHQCRTPVTPGHEVVGTVVATGARVGVPWLGWTCGECEYCRRGRENLCESARFTGCH
                     LDGGYAEYLVADERFCLPIPPRYSDADAAPLLCAGLIGFRSYRKIEDARVVGLYGFGA
                     AAHILAQIACAEGRTVFAFTRPGDDAGQRFALECGALWAGDSAQAPPEPLDAAIIFAP
                     VGALVVDALKNTRKGGRVICGGIHMSDIPSFSYADLWGERRIESVASLTREDGARFME
                     VLKKIVVKTETKTYPLAQANAALDDLRHGRFMGAAVLLP"
     gene            complement(4340..4483)
                     /gene="ccoS"
                     /locus_tag="VFB53_09765"
     CDS             complement(4340..4483)
                     /gene="ccoS"
                     /locus_tag="VFB53_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HZN87055.1"
                     /translation="MESLYLLVPVSVALVFLTGWAFWRALACGQFDDLERPAHDIVAD
                     DDS"
     gene            complement(4432..>6032)
                     /locus_tag="VFB53_09770"
     CDS             complement(4432..>6032)
                     /locus_tag="VFB53_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZN87056.1"
                     /translation="LAGTGEVYFDSISMLAFLLLGARYLESAARQRAAGALDPLLRRE
                     SQQPLLSGQTLQVAPGERIPADGVVLQGASTADESLLTGESRPVAKRRGDELVGGSVN
                     LEQPLVMRVTRAGTDTRAAAIARLAERGAASKPRLVDAAERVARHLTWIVVLVAALAG
                     AYFSSPWVAVAVLVVACPCALALAAPIILTRVSGFLLARGVLLTRAAALDALERVTDV
                     ALDKTGTLTLGHLVVARIFPLCDRDLHECYALAGALEASSRHPLARAFSGGDALAVRA
                     PRHVPGHGIEGTVDGRRVRIGSESFCQALCGAPPAGPAHPNFDSSFVFLADQEGWIAA
                     FELADRLRPDAAALVAELRKRNLSVHLATGDRPDVGAALARALWIENAAGAMTPEDKY
                     RYVENLQRQGRVVAMVGDGLNDAPVLARADVSFAMGGGADAAQLRADVVLMNDSLEAV
                     SQTLVLARRAMHLVRQNITWAVTYNAVALPLAALGWIGPWEAALAMGASSLTVLLNAL
                     RPLAAESPWKASTSSSLSRSPSYS"
BASE COUNT          993 a         2103 c         2146 g          790 t
ORIGIN      
        1 ctcggcgtat tccttcgacg aggtgatgcc cattggcagc gtgatcgcgg ccaggacgat
       61 gaccgcttgc cagccccaga aggtgaacgc ggctagcttg tcgcagaaga ggcgcgcgtg
      121 cgaagtacgc tgcaccacgt agtacgagga cgcgaacagc gcgcagccgc cgaaggcgaa
      181 gatcaccgcg ttggtgtgca gcgggcgcag cctgccgtag gaaagccacg gcacgccgaa
      241 gttgagctcg ggccagagaa gctgcgcggc gatcagcacg ccgaccgcca tcccgaccac
      301 gccccagacg atggtggcaa gcgcaaactg gcgcaccacc ttgtagttat aggaatcttc
      361 catgaacctc cgatgctagg cgcgggcgca gcgcggcgtt tgacctaggt caacctcggc
      421 tgcaacagga aacggccgta gtggaccgcg aacgtggcga aagccgcgct ccacgccagg
      481 ccggagagcg aggtgacgag cgccaaatgc gcgcccgaga atgcgctcag cacccgcagc
      541 acggcagcgg ccgacaccaa cgcgtagatc gtgcgggtgg ctccgtcggc cacaaggggg
      601 cgcccactgt ggccgagcga tgcgcgcgtc atgaccgcca gggtcatggt gccgatcgcg
      661 cccaccgtca gcgcgtgcag cgccgcgctc gagccgtcaa ctccgagaaa gacgagcccg
      721 agggcgagcc agccgtagcc gaggtgcagg acgaagagca gcggctcggc gagcgtcgcc
      781 tttccgcgcc agcgtgccag gcgcgccgtg agggccagtc ccgcgaccgc cagcggccac
      841 tcggcgactg cgaaaaccca agccgcgagc gcggcgagcg taaccagcag cgccgctcgg
      901 tcgagcacgc cttccggcgc cggcggcggc acgccgggac gcgccttcgc gagccagttg
      961 cgcgtaaagc tcggcacgat gcgcccgccg atgagggcga tgagcatgag cagcgtggcg
     1021 atgccgacac ggatgccgag catcgcgctg taggccacgc cgagcgcatg caggtgcacg
     1081 agtacgttgc ccgcgagcag caggcttagc gcggcgagca tcggcaggtt gcgccagttg
     1141 cgcccggcga ccagctcgcg cgcgaccacc gcgatcagcg cgacgggaaa cgcaaggtcg
     1201 agaactgcgg ccaccggagc gcctacgtgc cccgagatca gcaaaccgac ccggccgacg
     1261 aaccatagcg ccgcgagcgc ggcgagcgcg gcaccccgca ccggcagccg gccggtccag
     1321 ttcgggatcg cggtgagcag gaagccggcg acggcggcaa agccgaaacc gaacaccatc
     1381 tcgtgcgcgt gccagaagag cgcggggaga gtcccgcgca gcgcatagcc gtggacatag
     1441 gctgcaagcc acaccggcac ggcgagcgcg gcccaggccg acgcaaggag gaaaaacggc
     1501 cgaaaacctg ctgcgaacac tgccatgcgg caaagataag gagtacggtg gtgtcgaact
     1561 tgatccatct caaaccgggg taacctccgg cgcgcatgcc cggccgccgc atttccttcg
     1621 ccaccatcgc cgtgagcggc ctgggcgcgt tcgtcgcgct cgccgtgggc gcgacgctct
     1681 acgtcagcgc ctcgcgcggc ctgcgttcga cgcacgcgtg gatcgcgcag caggccgagg
     1741 cgcgcctcga cgcgctggag cagcggctcg aggcgcgact gcgcccggtc gccgaccaag
     1801 gcgagtcgat cgccgaagcg ttcgccgacg ggcgcatcga tcccgcccgg ccggccgtgc
     1861 tcgacgcatt catgttcggc gcgctcggcg ccacgccaca ggtctccgac ctcggcatcg
     1921 tcgatccctt cgggcgcacg cgccgctgga gccgcggggc gctcgccgcg agcagcgccg
     1981 actggtcgca gcgcccggag gttcgcaaat ggctcgtcgc cggccgcgag caggacgggc
     2041 cggcatggcg cccgctcgtc tgggaagacg ctgccgtgcg gggtccttcg ctgctgcacc
     2101 aggtcgcctt gcagcgcgcg ggcaacttcg tcggcatgct gggtcaggtc gtgcccatgg
     2161 cgaagctctc ggaggacctc gcggtcttcg gcgcggaata cggcgtcacc cctttcgtgc
     2221 tttatgggcc gcgagccgac ctggtggtcg cacaccgcgc tttcgccggc gcgggcacgg
     2281 catccggtgg cctgcccgcg atcgtggacg ctggcgatcc ggtgctcgcg cgcctgcatt
     2341 cgcccgatgg gccggcactg ctctcgggcg cgctcaagcg cgcgaaaggc gtgcgcgcga
     2401 gcgtgagcga gacgacctac gtattcctca cgcgcgaggt tcagcttcac gggcgcgagc
     2461 cctggaccat cggcttgtac ttcgacccgg tgcagggcgg gcagcgcgcc gagatgatgc
     2521 gcacgctgta ttcgatcggc gtcggcctcg cggtgctggc gctcgccgtg gcgctcgccg
     2581 cgatcgccgg tcgccggctc ggccggccga tcgaggcgct ggcgcgcgcc gcgcacctgg
     2641 tgcgaaaggg ccggctcggc gaagtgcaga cgctgccgcg cagccggatc tacgagctcg
     2701 acgaggccaa ccgctctttc gccgagatgg tcgaaggcct gcgcgagcgc aacgtgatgc
     2761 gcgagacgct cggccagttc ctgcccgagc aggtggcgct cgagctcctc gccggcggcg
     2821 ggcgcatcga accggaggaa gccaaggcca cggtgctgat cggcgacatc gagggcttcg
     2881 ccacgctcac cgacacgctc ggcgcgcacc gcacgatcga gttcctcaat gcgtacttcg
     2941 aggtaatggt cggcatcatc gagcgctacc gcggcgtggt cacccagttt cagggcgacg
     3001 cggtgctcgc ggtgttcaac gtgccgatcg ccgaccgcga ccacggcgcg aacgcgctgc
     3061 gcgccgcgct cgagatcgtg cgcgcggcac aggcgcagag cttcgccggg gtgcgcgcgc
     3121 tcaaccgtgt aggcatcagc accggccggg tggtggccgg ggcggtgggc gcggccgggc
     3181 gcctcactta cacggtgcac ggcaatgcgg tcaatctcgc cgcgcgcctg gaggcgctga
     3241 acaaggaata cggcacgcgc atcctgctct ccgacaagac cgcggagcgc tgcccgggct
     3301 tcaggctgcg caaagtcgcc gatgcgcaga tccggggcta cggcgcgccg gtcgcgctct
     3361 acacgcccga ggagcgctga ccgtccctaa ggcaggagca cggcggcgcc catgaagcgt
     3421 ccgtggcgca gatcgtcgag tgctgcattc gcctgcgcca gcggataggt cttggtttcg
     3481 gtcttgacga caattttctt cagcacctcc atgaaacggg cgccgtcttc gcgggtgaga
     3541 ctggcgaccg actcgatgcg gcgctccccc cagagatcgg cataagagaa ggacgggatg
     3601 tcgctcatgt ggatgccgcc gcagatgacg cgcccgcctt tgcgggtatt tttcagggca
     3661 tcgaccacga gggcgcccac gggagcgaag ataatcgccg cgtcgagcgg ctcgggcggc
     3721 gcctgcgccg agtcgccggc ccagagcgcg ccgcattcca gcgcaaagcg ctgcccggcg
     3781 tcgtcgcccg gccgtgtaaa ggcgaaaacg gtgcgccctt cggcgcaggc gatctgcgcc
     3841 aggatgtgcg ccgccgcgcc gaagccgtac aggccgacga ctctggcatc ttcgatcttc
     3901 cggtaactac gaaaaccgat tagtccagcg cagagcagcg gcgcggcatc ggcgtcgctg
     3961 tagcgcggcg ggatcggcag gcagaaacgc tcgtcggcca cgaggtactc cgcgtagccg
     4021 ccgtcgagat ggcagccggt aaagcgcgcg ctctcgcaca agttctcgcg tccgcgccgg
     4081 cagtattcgc attcaccgca ggtccatccc agccacggca cgccgaccct ggcgcccgtg
     4141 gccaccaccg tgccgacgac ctcatggccc ggagtcaccg gcgtgcggca ttgatgcagc
     4201 tcgccgtcgg caaggtgcag gtcggtgcgg cacacgccgc aggcgcgcac cttgatcagc
     4261 acctcgcctg gcccgggcat tggcacgtcg gtcgtttcgc agcgcaaggg ctgccgcggc
     4321 cccacgaggc gcatcgccct cacgagtcgt cgtccgcgac gatgtcgtgt gccgggcgct
     4381 cgaggtcgtc gaactggccg caggcgagcg cgcgccagaa cgcccagccg gtcaggaata
     4441 cgagggcgac cgagacaggg acgaggaggt agaggctttc catggacttt ccgcggcgag
     4501 cggccgcagc gcattcagca gaaccgtcag cgagctcgcg cccatcgcga gtgccgcttc
     4561 ccaggggccg atccagccga gcgcggcgag cggcagcgcg acggcgttgt aggtgacggc
     4621 ccacgtgatg ttctgacgga cgagatgcat cgcgcgccgc gcgagcacca aggtttgcga
     4681 gacggcttcg agcgaatcgt tcatcagcac gacgtcggcc cgcagctgcg cggcatcggc
     4741 gccgccgccc atcgcgaacg acacgtccgc gcgcgcgagc accggggcgt cgttcagacc
     4801 gtcgccgacc atggccacca cgcgcccctg tcgctggaga ttttccacat agcggtactt
     4861 gtcctcaggc gtcatcgcac cggccgcgtt ctcgatccag agcgcgcgcg cgagcgcggc
     4921 gccgacgtcc ggccgatccc cggtcgcgag gtgaacgctc aggttgcgct ttctcagctc
     4981 cgcaaccagc gccgcagcgt cggggcgcag gcggtcggcg agctcgaatg ccgcgatcca
     5041 gccttcctgg tccgcgagaa atacgaacga gctgtcgaag ttcgggtgcg ccggcccggc
     5101 gggcggcgcg ccgcagagcg cctggcagaa cgactcgctg ccgatccgca cgcgccgtcc
     5161 gtcgaccgtt ccctcgatgc cgtgccccgg cacgtggcgc ggagcgcgca cggcgagcgc
     5221 gtcgccgccg gaaaaggcgc gcgcgagcgg gtgccggctc gaagcctcga gcgcccccgc
     5281 cagcgcgtag cattcgtgca ggtcgcgatc gcacagcgga aaaatgcgcg ccacgacgag
     5341 gtggccgagc gtcagcgtac cggtcttgtc gagcgccacg tcagtcacgc gctcgagcgc
     5401 atcgagcgct gcggcgcgcg tcagcagtac gccgcgtgcg aggagaaagc cgctcacgcg
     5461 cgtgaggatg atcggcgcgg cgagcgccag ggcgcacggg caggcgacga ccagcaccgc
     5521 cacggccacc cacggacttg aaaaataggc tccggcgagc gccgcgacga gcacgacgat
     5581 ccaggtcagg tgacgcgcca cgcgctcggc cgcgtcgacg aggcgcggct tcgaagctgc
     5641 gccgcgctcg gcaagccgcg cgatggcggc ggcgcgcgtg tcggtgccgg cgcgcgtcac
     5701 gcgcatgacg agcggctgct cgaggttgac cgagccgccg accagctcgt cgccgcgacg
     5761 cttcgccacc ggccgggatt caccggtgag gagcgactcg tccgcggtgg aagcgccctg
     5821 caggacgacc ccgtcggccg ggatgcgctc tccgggagcc acctgaagcg tttgcccgga
     5881 caagagcggc tgttgcgatt cgcgtctcaa caaggggtcg agagcgccgg cggcgcgctg
     5941 gcgcgcggcg ctttcgagat agcgcgcgcc gagcagcagg aacgcgagca tgctgatcga
     6001 gtcgaagtag acctcgcctg tgccggcgag cg
//
LOCUS       DASRIE010000230         9521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2852849, whole genome shotgun sequence.
ACCESSION   DASRIE010000230 DASRIE010000000
VERSION     DASRIE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852849"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFB53_09775"
     CDS             complement(<1..897)
                     /locus_tag="VFB53_09775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN87057.1"
                     /translation="MRLAFVTGSLAHGGAERQTLALAERLAARGHACELVHVKGEAGL
                     VERLPPRAAAVRSLEAKRYLDRRALGALSRRFAALRPEAVVAANGYALLYAWLALRAA
                     RCAAPLVVTFHTTRLLDAKQALQMALYRLFFWSAQCTVFVCESQRRYWRRRGVFSRRT
                     AVIYNGVDTENYRDVCGPEERARLRAAFGFAPQDYVIGMSALLRPEKNHLQMVGAVAR
                     LRAQGIPARALMIGDGEMRGAVEARARELGVAGDVAITGLKHDVRPYLEACDVLALCS
                     LTEAFSLAAIEAMAFRRPVVHSA"
     gene            complement(901..2349)
                     /locus_tag="VFB53_09780"
     CDS             complement(901..2349)
                     /locus_tag="VFB53_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87058.1"
                     /translation="MSATRQLGRHALTLGAANAVEAGLQFLLPVVLVRCLAPEAFGEY
                     RLLWLAVGTVMALLTQAMPGSLYYFLPRSEAADKRLYINQALLVLLAAGAAAAWAVSA
                     WNPWLPEQLAALARHRYVVPAFVLLWVVASLLDLLPMVEERIAWQARSVAGLAALRSA
                     ALALAAALTGELAPVFAVLLGFVAFKVALLLWYIARFHGLRGPWLRWGALAHQVGTVA
                     PFAAASTLYSLRSQADQWVAAALFSVASFAAFSIGALLAPLLTLLRKSVSHVFLPGMS
                     RLQAGGDLRGMLEMNSRANVMVGALAFPTLAFAFAFAGDLVTLVFTATYLEAAPVMRV
                     YIAGLAVLVLELASLTFLLQQGGFVLRLGAVALALSVALSLLGAQAWGLAGAAAGSTA
                     AAYLDLAVTLRRVAQRTGVPLAELQDWRALGLLLLFAVLAAALAAALVGHFLAGGAPL
                     ARLALGGALLAAVYAAMAFSWPRGRGWLAAQR"
     gene            complement(2346..3767)
                     /locus_tag="VFB53_09785"
     CDS             complement(2346..3767)
                     /locus_tag="VFB53_09785"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZN87059.1"
                     /translation="MQRLLSGAAAQSPVALAGLALAGALAGAAIALADVKALILCAAL
                     VGCAFILLDFRIGVVLLIVLMPLSRSALFPHAMFGVTGLNPVNLLLAATFGAWLLQSL
                     GDGSWRRFLPRPLAWLYLAPLAAAAALGLRHVGDIVPDFYLYGLIEFQDVAGYLRDML
                     FKPLLLVLFALLVGAAVARSAKPEAFLVPACLSVWVMGAIVLGYVLLSGAGLGTLADG
                     GARGFLSPLGMHANELGRLYVSAYAMLLFTAAGTERSAARPLLAATLALVVVSLMLTF
                     SRGAFLGFMLVNVVFLVWRRSGTVLLLGALLATAAPLLVPEEVLERIGAGVGQGANAV
                     SAGRIDGLWLPLLPDLLRSPLYGSGLGSILWSGAMLDAQAGAPGIIAATHPHNAYLEA
                     LLDMGLLGLGLLCAYYLHVWRCLRALAADASLNPTLRGFFQGAAAGLVALLAVAVADS
                     SLAPRSEQAYLWLAIGLMLGCRR"
     gene            complement(3752..4639)
                     /gene="epsG"
                     /locus_tag="VFB53_09790"
     CDS             complement(3752..4639)
                     /gene="epsG"
                     /locus_tag="VFB53_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HZN87060.1"
                     /translation="MLSPEKVLPIEGASRVLARDDRRLGGILAAEGKLGEADVERVLR
                     LQQAQGLRFGEAALRLGLVSAEDLHRAIARQYDFPSPLAGAGAGAELVVARAPFHPCA
                     EELRALRTQLQIRWSNAGVSPRLLAIASPGPGEGRSYLAANLAVAFAQLGERTLLVDA
                     DLRAPRQHRIFGLRESAGLSAVLSGRADRGALLPLADFGPLSILPAGAVPPNPQELLS
                     RPVLAALLRELRAEFDVILFDTPPARHCADAQSVAFRAGHALVLARRHHTRLADASAV
                     VRELGDTGARVLGTVCNAF"
     gene            complement(4646..6022)
                     /gene="epsF"
                     /locus_tag="VFB53_09795"
     CDS             complement(4646..6022)
                     /gene="epsF"
                     /locus_tag="VFB53_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HZN87061.1"
                     /translation="MSPALFLSALRARLALAGVVLAATLLAAAALSLLLPKSYRASAS
                     LLVDAARDEQSLSNVLVPQRERIGYLQTQMDILASKRVALKAVQDLKLAGRPELRAAF
                     ADSTGGAGSFEDWLASELLRRLKVEVSQSNVIQLVYASADPEYAALVVNGVAQAYIDT
                     MLELRVAPTRQAALWFDEQLKSLRANLEDAQARLTSYHRQQGIISADERLDVENARLA
                     ELSSQLVKAQEQVIELRARERLPEAPAGARVQALQADLARAEAKLQELAPQYGARHPQ
                     MLRQEAEVGSLRAQLRAAVAEGRAGIAQSRRQSEQREAQLAAALAAQRARLLELKEGR
                     NELAVLTRDVETAQRTYETAMQRAVVSQVESRASQTNVALLSPAVAPREAAHPKLALN
                     LALALVAGTLLGVGLVVLLEMLERRVHAPGDLQAALDVPLLGALGTWRPATGLLGGAA
                     PPALAGPG"
     gene            complement(6030..6806)
                     /gene="epsE"
                     /locus_tag="VFB53_09800"
     CDS             complement(6030..6806)
                     /gene="epsE"
                     /locus_tag="VFB53_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HZN87062.1"
                     /translation="MLRALLAGAALLIAAAAAAAQTLGPGDSIRITVFQNPDLSVEAR
                     LSERGTISYPLLGEVELAGQTPEAAGKLIGARLRSGKFLRDPQVSVTLLQLRSRQVSV
                     LGQVARPGRYPLEEGSAKLTDVLALAGGIAPGGADKVTVSGTRDGAPVQQHVDVPALI
                     GGGADKVPEVQPGDTVYVPRAPQFYVYGEVQRAGAYRLEPDLRVMGALSLGGGLTPRG
                     TQRAPRIHRRMPDGSVKVLEARLTDAVQPDDVIYFRESLF"
     gene            complement(6810..6974)
                     /locus_tag="VFB53_09805"
     CDS             complement(6810..6974)
                     /locus_tag="VFB53_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87063.1"
                     /translation="MLTQTLTLLSLALLLAAHPLRHWEMPEPAAGSALLCGLLVISFM
                     AWRRSRWPEG"
     gene            7172..7672
                     /locus_tag="VFB53_09810"
     CDS             7172..7672
                     /locus_tag="VFB53_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87064.1"
                     /translation="MKLARSRLSRTQIEQLLEHFVAGTPARPAAALAGVNRNTSNAFY
                     RRLRELIARRMVLRAAPQAQFGLREANGAVRVIVDPVPEERCEALVRLRERRVLRATD
                     LALEASARNPRIDEFWLKLKQQLRRYNGMPRTQFHLFLAECEWRLRCGTPRHCLHVLR
                     HWLSRS"
     gene            8092..>9521
                     /locus_tag="VFB53_09815"
     CDS             8092..>9521
                     /locus_tag="VFB53_09815"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZN87065.1"
                     /translation="MKPRNLLLALLLGAAHAAPADAGSTKFIPTFLVYYGGGPTLTAA
                     DAPKLAKFDLIDIDRFRYNQIGGGTWSAIKAYNPDVQIYLYQMGPESSNYMDAAQQVS
                     LNNLGRYNVSRGHAMGSLNGNNAGLFLQDAGGQRIYNKAYSNTSQGTYWHLMDFGSAA
                     YQQYWVAGVKADIIDQPWAADGVFVDNCLTLSAAGGYSATAARYPSNAAWSAAMNAFT
                     GGIASGVHAYGQKLWCNKGDTASAEGVSAWLELDNGASPPDVLLEEGAFAVKWGDGAT
                     QFFPEQSWKRQVDVMGAMQNSAVAMLSHTDLLEGQSGTDNWGRPVSFGQTLWYALGSF
                     LLGKNDQRGNAYFTFVDGNDYNRIFWFEEYDRLDFGRALGAYRVTTIGGVNVYWREFE
                     RGYVYVNPTASDVASIALPQALRLLTRATLGTLAELLPVVNTIPLAGHHAAFVLKTTE
                     TPVADTQAPTVPAGLVATAVSGTQVNL"
BASE COUNT         1462 a         3536 c         3392 g         1131 t
ORIGIN      
        1 cgccgaatgc accaccggcc tcctgaacgc catcgcctcg atcgccgcga gcgaaaacgc
       61 ttccgtcagg ctgcacagcg ccagcacgtc gcaggcttcc aggtacggcc gcacgtcgtg
      121 cttcaggccg gtgatcgcca cgtcgccggc cactcccagc tcccgcgcgc gcgcctccac
      181 cgcgccgcgc atctcgccat cgccgatcat cagcgcgcgc gccgggatgc cctgcgcgcg
      241 cagccgcgcc accgccccga ccatctgcag gtggttcttt tccgggcgca gcagcgccga
      301 catgccgatc acgtagtcct gcggcgcgaa gccgaaggcg gcgcgcaggc gggcgcgctc
      361 ctcgggcccg cagacgtcgc ggtagttctc ggtatccacg ccgttgtaga tcaccgcggt
      421 gcgccgcgag aacaccccgc gcctgcgcca gtaccgccgc tggctctcgc acacgaacac
      481 ggtgcattgc gcgctccaga agaacaggcg atacagcgcc atctgcaggg cctgcttcgc
      541 gtccagcagg cgcgtggtgt ggaaggtgac caccagcggg gcggcgcagc gcgccgcgcg
      601 cagggcgagc caggcataca gcagggcgta gccgttggcc gccaccaccg cttcgggccg
      661 cagcgcggcg aagcgccgcg acagggcgcc gagcgcccgc cggtcgaggt agcgcttcgc
      721 ctccaggctg cgcactgccg ccgcgcgcgg cggcaggcgc tcgaccaggc ccgcttcgcc
      781 cttcacgtgc accagctcgc aggcgtgccc gcgcgcggcg agccgctcgg cgagcgcgag
      841 cgtctggcgc tcggcgccgc cgtgcgcgag cgagccggtg acgaaggcga ggcgcatcgc
      901 ctagcgctgg gcggcgagcc agccgcgccc gcgcggccag gagaaggcca tcgccgcgta
      961 gacagcggcg agcagcgcgc cgcccagggc gaggcgcgcc agcggcgcgc cgccggcgag
     1021 gaaatgtccg accagcgcgg cggcaagcgc cgcggccagc acggcgaaca gcagcagcag
     1081 cccgagcgcg cgccagtcct gcagctccgc gagcggcacg ccggtgcgct gcgcgacgcg
     1141 ccgcagggtg acggcgaggt cgaggtacgc cgccgcggtg ctgcccgcgg cggcgcctgc
     1201 gagcccccag gcctgcgcgc cgagcaggct cagcgccacc gacagcgcga gcgccacggc
     1261 gcccaggcgc agcacgaagc cgccctgctg caggaggaag gtgaggctgg cgagctcgag
     1321 caccagcacc gcgaggcccg cgatgtacac gcgcatcacc ggcgccgcct cgaggtaggt
     1381 ggcagtgaac accagcgtga cgaggtcgcc ggcgaaggcg aaagcgaaag cgagcgtggg
     1441 gaaggcgagc gcgcccacca tcacgttggc gcggctgttc atctcgagca tgccgcgcag
     1501 gtccccgccc gcctgcagcc ggctcatgcc cggcaggaac acgtggctca ccgacttgcg
     1561 cagcagcgtg agcagcggcg cgagcagcgc gccgatggaa aaggccgcga aactggccac
     1621 cgaaaagagg gccgccgcca cccactggtc ggcctgcgat cgcaggctgt agagcgtgct
     1681 cgcggcagcg aaaggggcga cggtgcccac ctggtgcgcg agcgcacccc agcgcagcca
     1741 gggcccccgc aggccgtgga agcgcgcgat gtaccacagc agcagcgcca ccttgaaggc
     1801 gacgaagccg agcagcaccg cgaacaccgg cgcgagctcc ccggtgagcg ccgcggcgag
     1861 cgcgagcgcc gcgctgcgca gtgcggcgag gccggcgacg ctgcgcgcct gccaggcgat
     1921 gcgctcctcg accatcggca ggagatccag gagcgaggcg accacccaca gcagcacgaa
     1981 ggcgggcacc acatagcggt gccgcgccag ggcggcgagc tgctccggca gccaggggtt
     2041 ccacgcgctc accgcccagg cggccgccgc gccggcggcg aggagcacca gcagcgcctg
     2101 gttgatgtac agacgcttgt ccgcggcctc cgagcgcggc aggaagtagt acaggctccc
     2161 cggcatggcc tgggtgagga gcgccatcac cgtgcccacc gccagccaca gcaggcggta
     2221 ctcgccgaag gcctcgggcg ccaggcagcg caccagcacc accggcagca ggaactgcag
     2281 gccggcctcc accgcgttcg ccgcgccgag ggtcagcgcg tgccggccga gctggcgcgt
     2341 ggcgctcacc ggcggcaccc gagcatcaat ccgatcgcca gccacaggta ggcctgctcc
     2401 gagcgcggcg cgaggctgct gtcggcgacg gcgacggcga ggagcgcgac cagccccgcc
     2461 gccgcgccct ggaagaaacc gcgcagcgtc gggttgagcg aggcgtcggc ggcgagcgcg
     2521 cgcaggcagc gccagacgtg caggtagtag gcacagagca gtccgaggcc gagcaggccc
     2581 atgtcgagca gcgcttcgag gtaggcgttg tgcggatgcg tggcggcgat gatgccgggc
     2641 gcccccgcct gcgcatcgag catggcgccg gaccacagga tcgatccgag gccgctgccg
     2701 tagagggggc tgcgcagcag gtcgggcagc agcggcagcc acagcccatc gatgcgcccg
     2761 gcgctcaccg cgttggcccc ctgcccgacc cccgcgccga tgcgctcgag cacctcctcg
     2821 ggcaccagca gcggggcggc ggtggcgagc agcgcgccga gcagcagcac cgtgccgctg
     2881 cgccgccaga cgaggaacac gacgttcacc agcatgaagc cgaggaaggc gccgcgcgag
     2941 aaggtgagca tcagcgaaac caccaccagc gcgagcgtgg cggcgagcag cggccgcgcc
     3001 gcgctgcgct cggtcccggc cgcggtgaac agcagcatcg cgtaagccga cacgtacagg
     3061 cgccccaact cgttggcgtg catgccgagc ggcgacagga agccgcgcgc gccgccgtcc
     3121 gccagcgtgc cgagccccgc gcccgagagc agcacgtagc cgagcacgat cgcgcccatc
     3181 acccagaccg acaggcaggc cggcacgagg aaagcctcgg gttttgccga gcgcgccacc
     3241 gccgcgccga ccagcagcgc aaacagcacc agcagcagcg gcttgaacag catgtcgcgc
     3301 aggtagccgg ccacgtcctg gaactcgatc aggccgtaga ggtagaagtc cggcacgatg
     3361 tccccgacgt ggcgcaggcc gagcgccgcg gcggcggcga gcggcgcgag atacagccac
     3421 gcgagcggcc gcggcaggaa gcgccgccag ctgccgtcgc ccagcgactg cagcagccag
     3481 gcgccgaacg tcgccgcgag cagcaggttc accgggttga ggccggtgac gccgaacatg
     3541 gcgtgcggga acagcgcgct gcgcgacagc ggcatcagca cgatcagcag caccacgccg
     3601 atgcggaagt cgagcaggat gaaggcgcag cccaccagcg ccgcgcacag gatgagcgcc
     3661 ttgacgtcgg cgagcgcgat cgcggcgccc gctagcgcac ccgccagcgc gagccccgcc
     3721 agcgcgacgg gcgactgcgc cgccgcgccg ctcagaaggc gttgcacacg gtgccgagga
     3781 cgcgagcgcc cgtgtcgccc agctcgcgca ccacggcgct cgcgtcggcg aggcgggtgt
     3841 ggtggcggcg cgcgagcacc agcgcatgcc cggcgcggaa ggccacgctc tgcgcgtcgg
     3901 cgcaatgccg cgccggcggc gtgtcgaaca ggatcacgtc gaactcggcg cgcagctcgc
     3961 gcagcagcgc cgccaggacc ggccgcgaca gcagctcctg cgggttgggc ggcaccgcgc
     4021 ccgccggcag gatcgacagc gggccgaagt ccgccagcgg cagcagcgcg ccgcggtcgg
     4081 cgcggcccga gagcaccgcc gacaagccgg cgctctcgcg caggccgaag atgcggtgct
     4141 ggcgcggcgc gcgcaggtcg gcatccacca gcagcgtgcg ctcgccgagc tgcgcgaagg
     4201 ccaccgccag gttggccgcc aggtagctgc gcccctcccc cggtcccggg ctggcgatgg
     4261 ccagcaggcg cgggctcacg ccggcgttgg accagcggat ctgcagctgg gtgcgcagcg
     4321 cgcgcagctc ctcggcgcag gggtggaagg gcgcgcgcgc caccaccagc tccgcgcccg
     4381 cgccggcgcc cgccagcggc gaggggaaat cgtactggcg cgcgatcgcg cggtgcagat
     4441 cctcggcgct caccaggccc agacgcagcg ccgcctcgcc gaagcgcagg ccctgcgcct
     4501 gctgcagccg cagcacgcgc tcgacgtcgg cctcgccgag cttgccctcg gcggcgagga
     4561 tgccgccgag gcgccggtcg tcgcgcgcca gcacgcgcga ggcgccctcg atcggcagga
     4621 ccttttcggg cgacagcacg cggcctcagc ccggaccggc cagcgccggg ggcgcggcgc
     4681 cgccgagcag tccggtggcg ggtctccagg tgccgagcgc gccgagcagc ggcacgtcca
     4741 gcgcggcctg caggtcgccc ggcgcgtgca cgcggcgctc gagcatctcc agcagcacca
     4801 ccaggccgac gccgagcagg gtgccggcca ccagcgccag cgcgaggttg agcgcgagct
     4861 tcggatgcgc agcctcgcgc ggcgccactg ccgggctgag cagcgccacg ttggtctggc
     4921 tggcgcggct ctccacctgg ctcaccaccg cgcgctgcat cgcggtctcg taggtgcgct
     4981 gcgcggtctc gacgtcgcgc gtgagcaccg ccagctcgtt gcgcccctcc ttcagctcga
     5041 gcagccgcgc gcgctgcgcg gcgagcgccg cggcgagctg cgcctcgcgc tgctcgctct
     5101 ggcgccgcga ctgcgcgatg ccggcgcgcc cttcggcgac cgccgcgcgc agttgcgcgc
     5161 gcaggctgcc gacctccgct tcctggcgca gcatctgcgg atgccgggcg ccgtactgcg
     5221 gcgcgagctc ctgcagcttc gcctcggcgc gcgccaggtc cgcctgcagg gcctgcaccc
     5281 gcgcgccggc cggggcctcg ggcaggcgct cgcgcgcccg cagctcgatc acctgctcct
     5341 gcgccttcac cagctggctg gagagttcgg cgaggcgcgc gttctcgacg tcgaggcgct
     5401 cgtcggccga gatgatgccc tgctggcggt ggtagctcgt cagccgcgcc tgcgcgtcct
     5461 cgaggttggc gcgcaggctc ttcagctgct cgtcgaacca cagcgccgcc tggcgcgtgg
     5521 gcgcgacgcg cagctcgagc atggtgtcga tgtaggcctg cgcgacgccg ttcaccacca
     5581 gcgccgcgta ctcggggtcg gcggaggcat agacgagctg gatcacgttg ctctgcgaga
     5641 cctccacctt gagccggcgc agcagctcgc tcgccagcca gtcctcgaac gagcccgcgc
     5701 cgccggtgct gtcggcgaag gccgcgcgca gctcggggcg cccggcgagc ttcaggtcct
     5761 gcaccgcctt gagcgccacg cgcttgctgg cgaggatgtc catctgcgtc tgcaggtagc
     5821 cgatgcgctc gcgctgcggc accagcacgt tgctcagcga ctgctcgtcg cgcgccgcgt
     5881 ccaccagcag cgaggcgctg gcgcggtagg acttgggcag caggaggctg agcgcggcgg
     5941 cggcgagcag cgtggccgcg agcaccacgc cggccaatgc caggcgcgcg cgcaatgccg
     6001 acaggaacag cgccggactc atgcgcgccc tagaacaggc tctcccggaa atagatcacg
     6061 tcgtcgggct gcaccgcgtc ggtgagccgc gcctccagca ctttcaccga gccgtcgggc
     6121 atgcggcggt ggatgcgcgg cgcgcgctgg gtgccgcgcg gggtcagccc gccgcccagc
     6181 gacagcgcgc ccatcacgcg caggtcgggc tcgagccggt aggcgccggc gcgctgcact
     6241 tcgccgtaga cgtagaactg cggcgcgcgc ggcacgtaca ccgtgtcgcc gggctgcacc
     6301 tcgggaacct tgtccgcgcc gccgccgatc agcgccggca cgtcgacgtg ctgctgcacc
     6361 ggcgccccgt cgcgcgtgcc gctgacggtc accttgtccg cgcctcccgg ggcaatgccc
     6421 ccggcgagcg ccagcacgtc ggtcagtttc gcgctgccct cctcgagcgg atagcgtccg
     6481 ggccgcgcca cctggccgag cacggacacc tgccggctgc gcagctgcag cagcgtcacg
     6541 ctcacctgcg ggtcgcgcag gaacttgccg ctgcgcagcc gggcgccgat cagcttgccc
     6601 gccgcctccg gcgtctgccc ggcgagctcc acctcgccga gcagcgggta gctgatggtg
     6661 ccgcgctccg acaggcgcgc ttcgacgctc aagtcggggt tctggaatac cgtgatgcgg
     6721 atgctgtcgc ctgggccgag cgtctgcgcg gcggccgcgg cggcggcgat cagcagcgcg
     6781 gcgcccgcga gcagcgcgcg cagcaccgtt cagccctcgg gccagcggct gcggcgccag
     6841 gccatgaagg agatgaccag caggccgcag agcagcgccg aacctgcggc gggctcgggc
     6901 atctcccaat ggcgcagcgg atgcgcggcg agcagcaggg caagcgagag caaggtgagg
     6961 gtttgggtaa gcatgcgaga gatactgggc cccggccatg acatgaccgt gctggccgga
     7021 ggccttgcgc atgccctgga cggattagtg ggaacagcgg cagagcagtc gttcaggcac
     7081 tacaggccgt cgtgattccg cgacagggcc aagacttccg ggggatggca tagaacacag
     7141 cgtgcaataa gtccacccgc atgctggtgg cgtgaaactc gcccgctcca ggctgagccg
     7201 cacgcagatc gagcagttgc tggagcattt cgtcgccggc accccggcgc ggccggcggc
     7261 cgcgctggcg ggggtcaacc gcaatacctc caacgccttc taccggcgcc tgcgcgagct
     7321 gatcgcgcgg cgcatggtgc tgcgcgccgc gccgcaggcg cagttcgggt tgcgcgaagc
     7381 aaacggcgcg gtgcgcgtca tcgtcgatcc cgtcccggag gagcgctgcg aggcgctggt
     7441 gcgcctgcgc gagcggcggg tgctgcgcgc caccgacctc gcgctcgagg cgtcggcgcg
     7501 caacccgcgc atcgacgaat tctggctcaa gctgaagcag cagctgcgcc gctacaacgg
     7561 catgccgcgc acgcagttcc acctgtttct cgccgagtgc gaatggcgcc tgcgctgcgg
     7621 cacgccgcgg cactgcctgc acgtcctgcg ccactggctg tcgcgcagct aggccatcgt
     7681 cgctgacgcg cgccgcgcgc tgcgcaaata cgcgcgccgc gcggtcgtgc gcgcgcaacg
     7741 tgcatagttc ctgcgcgcca tcgcgccccc gccaagcgct tgattcatcg agcaccgctt
     7801 tccgggcacg gagattgcac ctttccctcg tgatggcggg cggcaacgac cggcgttgcc
     7861 gcccgccttt ccagctgatc tggggggtgg acatgaaatc cgtcacgcaa gtcacttagc
     7921 gcccgcgcgc ggcttcctga gccgcctgcg gattctcccg ggccgcctca ggcattaccc
     7981 gagttcgtga agcaggtcac cgagccgctt gcgcccggcg cctagcatgc gcccgcgctt
     8041 gtagtcgacc tacgacattg ccggactttt tcaactactc atggattgac gatgaaacca
     8101 agaaacttgc tcctcgccct gctgctgggc gccgcccacg ccgcacccgc cgacgcgggc
     8161 tcgacgaagt tcattcccac tttcctcgtg tattacggcg gcgggcccac gctgactgcg
     8221 gccgacgcgc cgaagctggc gaagttcgac ctcatcgaca tcgaccgctt ccgctacaac
     8281 cagatcggcg gcggcacctg gagtgcgatc aaggcttaca accccgacgt gcagatctac
     8341 ctgtaccaga tggggccgga atcctccaac tacatggacg ccgcgcagca ggtgtcgctc
     8401 aacaacctcg gccgctacaa cgtatcgcgc ggccacgcca tgggcagcct gaacggcaac
     8461 aacgcggggc tgttcctgca ggacgccggc gggcagcgca tctacaacaa ggcctattcg
     8521 aacacctcgc agggtactta ctggcacctg atggacttcg gcagcgccgc ctaccagcag
     8581 tactgggtcg ccggcgtgaa ggccgacatc atcgaccagc cctgggccgc cgacggcgtg
     8641 ttcgtcgaca actgcctcac gctgtccgcc gccggcggct acagcgcgac tgcggcgcgc
     8701 tatcccagca acgccgcgtg gtcggccgcg atgaacgcgt tcaccggcgg catcgcctcc
     8761 ggggtgcacg cctacgggca gaaactctgg tgcaacaagg gcgatacggc ctcggccgag
     8821 ggagtctccg cctggctcga attggacaac ggcgccagcc cgccggacgt gctgctcgaa
     8881 gagggcgcgt tcgccgtcaa gtggggcgac ggcgccacgc agttcttccc ggaacagagc
     8941 tggaagcgcc aggtcgacgt gatgggcgcg atgcagaatt ccgcggtcgc catgctcagc
     9001 cacaccgacc tgctcgaggg ccagagcggc accgacaact ggggccgccc ggtcagcttc
     9061 gggcagaccc tgtggtacgc gctgggctcg ttcctgctcg gcaagaacga ccagcggggc
     9121 aatgcctact tcaccttcgt cgacggcaac gactacaacc ggatcttctg gttcgaggag
     9181 tacgaccgcc tggacttcgg ccgcgcgctg ggcgcctacc gcgtcaccac catcggcggc
     9241 gtgaacgtgt actggcgcga attcgagcgc ggctacgtgt acgtcaaccc caccgccagc
     9301 gacgtcgcct ccatcgcgct gccgcaggcc ctgcgcctgc tcacgcgcgc gacgctcggc
     9361 acgctggccg agctgctgcc ggtggtcaac acgatcccgc tcgccggcca ccacgccgcg
     9421 ttcgtgctga agacgacgga aacgccggta gctgacacgc aggcgccgac ggtgcccgcc
     9481 ggcctcgtgg cgaccgcggt gtccggcacg caggtgaacc t
//
LOCUS       DASRIE010000231         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1005259, whole genome shotgun sequence.
ACCESSION   DASRIE010000231 DASRIE010000000
VERSION     DASRIE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005259"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFB53_09820"
     CDS             complement(<1..762)
                     /locus_tag="VFB53_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZN87066.1"
                     /translation="MNQPTPPQAKRHLGQILIDQGVLTEDQLRIALLEQTKHHMPVGK
                     LLVQLGFVSEATLRDALSEKLGLQSVDLSRIIVDPAALKHLPRDMARRNRMFPVALDR
                     QQRKFIVALADTGNIVAIDQLRAHLKGDYEIELRLAGDAEIERATEQYYGHEFSIDGI
                     LKEIETGEVDYTTVSEGDEYSQPVVRLISALLADAVERGGSDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRIKVMAKMNIAETRAP"
     gene            complement(764..2083)
                     /locus_tag="VFB53_09825"
     CDS             complement(764..2083)
                     /locus_tag="VFB53_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN87067.1"
                     /translation="MSLLLEALKKAERAKEEAQRQARGDADGAADAAGKSEPAAPPVR
                     TRDQLPDINQPLEILSEDMAPPRPAPALALQEEKQEEKRPPRTPQAAPRAQAAAGDPQ
                     ATDRAAARKVFEAKFKEPNPRLPFFITMGALGVFAVGTVVYFWIQLRPAPSLVNANPP
                     RQAAEARVAAVETRPAPLAATGAQSAIPGLPAAPAAPQPAQAAPAPAKPAAPRPLAET
                     SRVEVPRLQPDPRRVAPRAEPETEVRSSRAAPQVHPKVDAGYAAYGAGDLGAARANYQ
                     DALREEPSNRDALLGLAALDVRAGRHEAAEAAYLRLLQIDPRDAHAQAALMQLRGGRV
                     DPAAASSRVKLMLAENPGAHALNFTLGNQLAQQGRWAEAQQEYFKAFGAEPDNADYAY
                     NLAVSLDHLRQPGLALDYYKRALALAKARGAGFDVAAAETRIAQLAR"
     gene            complement(2085..3740)
                     /gene="mshL"
                     /locus_tag="VFB53_09830"
     CDS             complement(2085..3740)
                     /gene="mshL"
                     /locus_tag="VFB53_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HZN87068.1"
                     /translation="MAETHLKPEEKPAGAIPPPVQITPLLPQPRPVARPETYSVVVSN
                     VRAQELLFALARDARLNIDIHPDITGTVTLNAIDQTLPQLLTRISRQVDMRYEINGQN
                     LIVMRDSPFLRVYRVDYVNITRDTRSVATVSTQVSGSQPGQTVTGAPNNSTATMNAVS
                     ANKFWDTVVANIKDILRETDKTIPKGASVPVAAAPAPTASGTTPAAAPPPQSQTEFIE
                     TAYVIANPETGILNVRATARQHEKVQEFLDQVMTSAKRQVLIEVTVAEVQLNNEYQRG
                     IDWQRLRTGATQTGVPGFATGQSGMEFGQSSANTPAGITTNAFVIGGAITSLNFNFAL
                     KLLESFGDVRVLSSPKISVLNNQTAVLKVVDNLVYFTTKADTTQNQTGSLTTFTTTLN
                     SVPVGFIMSLVPQISDADNVVLNLRPMISRKIGDVADPNPSLKLAGVTSNIPVIQTRE
                     MESVLRVQSGQIAVLGGLMQDARSSVEDGIPGVNRIPGLGAIFEQRRDLNQKTELVIF
                     LRTVVIRDASVEGDYAAFRALLPGSDFLTKPNPSGRPRSGAFE"
     gene            complement(3902..>4644)
                     /locus_tag="VFB53_09835"
     CDS             complement(3902..>4644)
                     /locus_tag="VFB53_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87069.1"
                     /translation="LADKSLLAAYTENTHAIVESHVRAAVADSEFAAVRRPARSALYL
                     AGAVAAGVVIGAAVQWMMSPAPAKAPPVQAPMAAPVPAAQASPAPAPVPEPVKAEAAP
                     APEPVAQPKPEQVRQPRRHLRADQARRITGYSADGQPLLGERLAATRTVLERAGDERH
                     VIELFITSNADPARMEGFLARARRVVPLEDLYVIPVAGGSAYRLWVVYGNFSSREEAA
                     AAGRRLPPRYQEAFPAAPRSFAELRRQL"
BASE COUNT          604 a         1558 c         1694 g          788 t
ORIGIN      
        1 cggcgcgcgc gtctcggcga tgttcatctt cgccatcacc ttgatgcgca ccgccatcgc
       61 cggccagtag gtcttgtgca gcgagcggat ctggcgcagc acgccgtcga tccggtagcg
      121 gatgcgcagg aagttctgct cgggctcgaa gtggatgtcc gagccgccgc gctcgaccgc
      181 gtcggcgagc agcgccgaga tcaggcgcac caccggctgc gagtactcgt cgccctcgga
      241 gaccgtggtg tagtcgacct cgccggtctc gatctccttg aggatgccgt cgatcgagaa
      301 ctcgtggccg tagtattgct cggtggcgcg ctcgatctcg gcgtcgccgg cgagccgcag
      361 ctcgatctcg tagtcgccct tgaggtgcgc gcgcagctgg tcgatggcga cgatgttgcc
      421 ggtgtcggcg agcgcgacga tgaacttgcg ctgctggcgg tcgagcgcca ccgggaacat
      481 gcggttgcgc cgcgccatgt cgcgcggcag gtgcttgagc gccgccgggt cgacgatgat
      541 gcgcgacagg tccaccgact gcagcccgag cttctccgac agcgcgtccc gcagcgtggc
      601 ctcggagacg aagccgagct gcaccaggag cttgccgacc ggcatgtggt gcttggtctg
      661 ctcgagcagc gcgatgcgca gctggtcctc ggtcaggacc ccctggtcga tcaggatctg
      721 tccgaggtgg cgcttcgcct gcggcggcgt gggctggttc acgtcagcgc gcgagctggg
      781 cgatgcgcgt ttcggcggcg gccacgtcga agcccgcgcc gcgcgccttc gcgagcgcca
      841 gcgcgcgctt gtagtagtcg agcgcgagcc ccggctggcg cagatggtcg aggctcaccg
      901 ccaggttgta ggcgtagtcg gcgttgtccg gctcggcgcc gaacgccttg aaatactcct
      961 gctgcgcctc ggcccagcgt ccctgctgcg cgagctggtt gccgagggtg aagttcagtg
     1021 cgtgggcgcc gggattctcg gcgagcatca gcttgacgcg gctcgacgcc gccgccgggt
     1081 ccacgcgccc gccgcgcagc tgcatcagcg ccgcctgggc atgggcgtcg cgcgggtcga
     1141 tctgcaggag gcgcagatac gcggcctcgg cggcctcgtg gcggccggcg cgcacgtcga
     1201 gcgcggcgag gccgagcagc gcgtcgcggt tcgagggctc ctcgcgcagc gcgtcctggt
     1261 agttcgcgcg cgccgcgccg aggtcgccgg cgccatacgc agcgtagccc gcgtctacct
     1321 tgggatggac ctggggtgcg gcgcggctcg agcggacttc ggtctccggc tcggcgcgcg
     1381 gcgccacgcg tctcggatcg ggctgcagcc gcggcacctc cacgcgggaa gtctcggcga
     1441 gcgggcgcgg cgctgccggc ttggccggcg cgggcgcggc ttgcgccggc tggggcgccg
     1501 cgggggccgc gggcaaaccg gggatcgcgg actgcgcgcc cgtcgccgcc agcggcgcgg
     1561 gcctggtctc gaccgcagcg acccgggctt cggccgcctg ccgcggcggg ttcgcgttca
     1621 cgagcgacgg cgccggccgc agctggatcc agaaatacac cactgtgccg acggcgaaca
     1681 cgccgagcgc gcccattgtg atgaagaaag gcagccgcgg gttgggttcc ttgaacttcg
     1741 cctcgaacac cttgcgggcc gcggcgcggt cggtggcctg cggatcgcca gcggcggctt
     1801 gggcgcgcgg cgcggcctgc ggcgtgcgcg gcgggcgttt ctcttcctgc ttttcctcct
     1861 gcagcgcgag cgccggcgcg ggccgcggcg gcgccatgtc ctcgctcagg atttccagcg
     1921 gctgattgat gtcggggagc tggtcgcgcg tgcgcaccgg gggcgccgcg ggctcggact
     1981 tccccgcggc atcggcagcc ccatcggcgt cgccgcgggc ttgccgctgc gcttcctcct
     2041 tggccctttc cgccttcttg agcgcctcga gcagcaggct catgtcattc gaacgcgccg
     2101 ctgcgtggcc gccccgacgg gttgggcttg gtcaggaaat ccgatcccgg cagcagggcg
     2161 cggaacgccg cgtagtcgcc ttcgacgctc gcgtcgcgga tcaccacggt gcgcaggaaa
     2221 atcaccagct cggtcttctg gttgagatcc cggcgctgct cgaagatcgc gccgaggccg
     2281 ggaatccggt tgacgcccgg gatgccgtcc tcgacgctcg agcgcgcgtc ctgcatcagc
     2341 ccgccgagca ccgcgatctg cccgctttgc acgcgcagca ccgactccat ctcgcgcgtc
     2401 tggatcactg gaatattgct ggtcacgccg gccagcttca agctcgggtt cggatctgcg
     2461 acgtcgccga tcttgcgcga gatcatcgga cggaggttga gcaccacgtt gtccgcgtcc
     2521 gagatctgcg gcaccaggct catgatgaag ccgaccggca ccgagttgag cgtggtggtg
     2581 aaggtggtga gcgagccggt ctggttctgg gtggtgtcgg ccttggtggt gaaatacacc
     2641 aggttatcca cgactttcag caccgcggtc tgattgttga gcacgctgat tttcgggctg
     2701 gagagcaccc gcacgtcgcc gaacgactcg agcagcttga gcgcgaagtt gaagttcagg
     2761 ctggtgatcg cgccgccgat cacgaaggcg ttggtggtga tccccgccgg cgtattcgcc
     2821 gaggactggc cgaactccat gccgctctgg ccggtggcga agccgggcac gccggtctgc
     2881 gtcgccccgg tgcgcaggcg ctgccagtcg atgccgcgct ggtactcgtt gttgagctgc
     2941 acctcggcga ccgtcacctc gatcagcacc tggcgctttg cgctggtcat cacctggtcg
     3001 aggaactcct gcactttctc gtgctggcgc gcggtggcgc ggacgttgag gatcccggtc
     3061 tccgggttgg cgattacgta ggccgtctcg atgaactcgg tctgggactg aggcggcggc
     3121 gcagccgcag gcgttgtccc ggacgcagtg ggtgccggtg ccgctgcgac cggcacggat
     3181 gcgcctttcg ggatggtctt gtcggtctcg cgcaggatgt ccttgatgtt cgcgaccacc
     3241 gtgtcccaga acttgttggc cgaaactgcg ttcatggtcg cggtggaatt attcggcgcc
     3301 cccgtcaccg tctggccggg ctggctgccg ctcacctgcg tcgacacggt tgcgacgctg
     3361 cgcgtgtcgc gcgtgatatt cacgtagtcc acccggtaga cgcgcaggaa gggcgaatcg
     3421 cgcatcacga tcaggttctg gccgttgatc tcgtagcgca tgtcgacctg gcgcgagatg
     3481 cgggtgagga gctggggcag cgtctggtcg atcgcgttca gcgtcaccgt gccggtgatg
     3541 tcgggatgga tgtcgatgtt caggcgcgcg tcgcgcgcca gcgcgaacag cagctcctgt
     3601 gcgcgcacgt tgctcaccac cacgctgtag gtctcgggcc gcgcgaccgg cctgggctgc
     3661 ggcagcagcg gagtgatctg caccggcggc gggatcgcgc cggcgggctt ctcctcgggt
     3721 ttgagatgtg tttccgccac cttgaccggt gcctggctgc agccaaacag cagtgcaatc
     3781 gcgccggccg caatcgcttt ccgcatcttc ataggggaac tgcctcccgc ggcaaggata
     3841 acacgctgcg ggtctcagga aagtgcggta agcgcctgtc gcacagggac tttccgcaat
     3901 tttacaactg ccggcggagc tcggcgaagc tgcgcggtgc ggcggggaat gcctcctgat
     3961 agcggggcgg cagccggcgc ccggctgccg cggcctcctc gcggcttgag aagttgccgt
     4021 acacgaccca cagccggtat gcgctgccac ccgctaccgg gatcacgtac aggtcctcga
     4081 gaggcaccac ccggcgcgct ctggcaagga agccctccat gcgcgccggg tcggcgttgc
     4141 tggtgatgaa gagctcgatc acgtggcgct cgtcaccggc gcgctcgagc accgtgcgcg
     4201 tcgccgccag ccgctcgccg agcagaggct gcccatcggc cgagtagccg gtgatgcgcc
     4261 gcgcctggtc ggccctgaga tggcgcctcg gctggcgcac ctgctcgggc ttcggctgcg
     4321 caacaggttc cggcgcggga gcggcttctg ccttgaccgg ttccggcacc ggcgccggag
     4381 ccggggatgc ctgcgccgcc ggcaccgggg cggccatggg ggcctggacg ggcggcgcct
     4441 tcgccggcgc gggggacatc atccactgca ccgcggcgcc gatgaccacg ccggcggcga
     4501 ccgcgccggc gagatagagc gcggagcgcg ccggacgcct gacggccgcg aactcggaat
     4561 cggcgaccgc cgcgcgcacg tggctctcga cgatcgcatg cgtgttctcg gtgtacgcgg
     4621 cgagcagcga cttgtcggcg agga
//
LOCUS       DASRIE010000232         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3637424, whole genome shotgun sequence.
ACCESSION   DASRIE010000232 DASRIE010000000
VERSION     DASRIE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637424"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFB53_09840"
     CDS             complement(<1..825)
                     /locus_tag="VFB53_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN87070.1"
                     /translation="MVFAWQRGAAVTAREFEAAAQALSARLPRKPYVVNLCEDRYAFT
                     VAFAASLIARQTTLLPPSRAPEAVRDSCAGHDAYTLTDGDVFQGEGPAAGKAFSVDPA
                     HVAAIVFTSGSTGRPVPHAKTWGSLAAGARALAERIQAAGTIVGTVPPQHMFGLEATV
                     MLPWQNGLAMHSGRPLLPADLGAALAEAGAPCWLMTSPLHLRACVAARQPLPGLAGVL
                     SSTMPLDAQLAQDAEKLWGAPVFEIYGSSEAGMIATRRTARDEAWELCPGVQLTERG"
     gene            complement(836..1399)
                     /locus_tag="VFB53_09845"
     CDS             complement(836..1399)
                     /locus_tag="VFB53_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87071.1"
                     /translation="MFAAYPAVAHFGKPMTALALLAALVAYLLAAAWIHHPVRWLAPP
                     AVGGAVYWLAPPAEWLLFIPPIAINLALAWLFGRTLLRGRVPLISRFALMEQETLSPE
                     VAGYTRLLTWLWTALFVAAAAISLVLAVSGHHDAWSLFTNLINYLLVGALFLGEFAYR
                     RVRFRNYRHHSPLQLLRNVRRTKLFER"
     gene            complement(1437..1712)
                     /locus_tag="VFB53_09850"
     CDS             complement(1437..1712)
                     /locus_tag="VFB53_09850"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZN87072.1"
                     /translation="MSLVQSAPLERELAAMLVEVLHLEMAAGDIDPQAPLYGDQGLGL
                     DSIDILEIAVAISKKYGFKLRSDDPDNDRIFASLRALASHIAEKRGQ"
     gene            complement(1717..>2071)
                     /locus_tag="VFB53_09855"
     CDS             complement(1717..>2071)
                     /locus_tag="VFB53_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87073.1"
                     /translation="VLFAGCKAAPIYEVVSAPIPAPAGKALTLDDVQKAIARGASRSG
                     WQLQPESPGHLTARFQSGRHSAAVNIDHDTKAYSIKMRDTSVRNDGTSVHPAYNNWVQ
                     SLDRAIRIELSSIGQ"
BASE COUNT          347 a          688 c          750 g          286 t
ORIGIN      
        1 gccgcgctcg gtcaattgca cgccggggca cagctcccag gcctcgtcgc gcgcggtgcg
       61 gcgcgtggcg atcatgcccg cctcgctcga gccgtagatc tcgaacaccg gcgcgcccca
      121 cagcttttcc gcgtcctggg caagctgggc atcgagcggc atggtcgagc tcagcacgcc
      181 ggccagcccg ggaagcggct ggcgcgcggc tacgcaggcg cgcagatgca gcggcgaagt
      241 catcagccag cacggcgcgc cggcttcggc cagagcggcg ccgagatcgg ccggcagcag
      301 cggccggccg gaatgcatcg ccaggccgtt ctgccacggc agcatcacgg tggcttccag
      361 cccgaacatg tgctgcggcg ggacggtgcc gacgatcgtg cccgcggcct ggatgcgctc
      421 ggccagtgcg cgcgcgccag cggcgagcga gccccaagtc ttcgcatgcg gcaccggccg
      481 gccggtgctg cccgaggtga acacgatcgc ggcgacgtgt gccggatcga ccgagaacgc
      541 cttgcccgcc gcagggcctt cgccctggaa cacgtcccca tcggtcagcg tgtaggcgtc
      601 gtgtccggcg caggaatcgc gcactgcttc gggcgcgcgg ctcggcggca gaagcgtggt
      661 ctgccgggcg atcaggctcg cggcgaaagc cacggtaaac gcatagcggt cttcgcagag
      721 gttcaccacg taaggcttgc gcggcagccg cgcggacagc gcctgcgccg ccgcttcgaa
      781 ttcccgcgcg gtgaccgcgg caccgcgttg ccaggcgaag accatgggcg tcatcctagc
      841 gctcgaagag cttggtccgt ctcacgttgc gcagcagctg cagcggcgaa tggtgccggt
      901 agttgcgaaa gcgcacgcgg cggtaggcaa actcgccgag aaacagcgcg ccgaccagga
      961 ggtaattgat cagattggta aacagcgacc atgcgtcgtg gtgccccgac acggccagga
     1021 cgagggagat cgcggcggcg gcgacgaaga gtgcggtcca cagccaggtg aggaggcggg
     1081 tatagcccgc gacttccggg ctcagggtct cctgttccat cagcgcgaag cgcgagatca
     1141 gcggaacccg cccgcgcagc agcgtgcggc cgaacagcca ggcgagcgcc aggttgatgg
     1201 cgatgggcgg aatgaagagc agccattcgg caggcggggc aagccagtag accgcgccgc
     1261 cgacggcggg cggcgccagc cagcgcaccg ggtggtgaat ccaggcggcg gcgagcaggt
     1321 aggcgaccag cgcagcgagc agcgcgagcg cggtcatcgg cttgccgaag tgcgcgaccg
     1381 cgggataagc ggcgaacaag cagagtccca gcaaccgcca cgcgcccacg gcgagactac
     1441 tggccgcgtt tctccgcaat gtgcgaggcc agggcgcgca acgacgcaaa gatgcgatcg
     1501 ttgtcgggat cgtcggagcg cagcttgaag ccgtatttct tggaaatcgc caccgcgatc
     1561 tccaggatgt cgatcgaatc gaggccgaga ccctggtcgc cgtacagcgg cgcttgcggg
     1621 tcgatgtcgc ctgccgccat ctcgaggtgc agcacctcga ccagcatggc ggcgagctcg
     1681 cgttcgagag gggcggattg caccaagctc atcgcctcat tggccgatcg acgaaagctc
     1741 gatgcggatc gcgcgatcca ggctctgcac ccagttgttg taggcagggt gcaccgaggt
     1801 gccgtcgttg cgcacgctgg tgtcgcgcat cttgatgctg taggccttgg tgtcgtggtc
     1861 gatattcacg gcggcgctat gccggccgct ctggaagcgc gcggtcaggt ggccgggcga
     1921 ctcgggctgc agctgccagc ccgagcgcga ggcgccgcgc gcgatcgcct tctgcacgtc
     1981 gtcgagcgtc agcgccttgc cggccggcgc gggaatcggc gccgacacca cctcgtagat
     2041 cggcgccgcc ttgcagccgg cgaacagcac c
//
LOCUS       DASRIE010000233         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1294426, whole genome shotgun sequence.
ACCESSION   DASRIE010000233 DASRIE010000000
VERSION     DASRIE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294426"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFB53_09860"
     CDS             <1..341
                     /locus_tag="VFB53_09860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZN87074.1"
                     /translation="LWVLGRLIDQPWFYAGLNAGDPAAAKGLLLFVLVIPAFTFFLQP
                     LASLYSRRHEFEADAYAAAHASAAELVRALVKLYQDNAATLTPDPLHSAFYDSHPPAA
                     TRIARLQAGT"
     gene            350..679
                     /locus_tag="VFB53_09865"
     CDS             350..679
                     /locus_tag="VFB53_09865"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZN87075.1"
                     /translation="METLAKRKCKPCEGGVPPYSPQQARELLQQLKGWILEEGKLVKV
                     FAFANYHHTMAFVNALAWVSHREDHHPDLEVGYNKCRVAYWTHAVGGLSENDFICAAK
                     CDALFEL"
     gene            complement(690..1595)
                     /locus_tag="VFB53_09870"
     CDS             complement(690..1595)
                     /locus_tag="VFB53_09870"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HZN87076.1"
                     /translation="MSAIAIIAALLLEQWRPLGERKTVFALLARWANWLERSFNAGAA
                     RHGMIAWLVAVVPALAAAVALHYLLLWASPLLAIAFNVAALYLTLGFRQFSHYFTDLQ
                     LAIRAGDVDRARELIGAWRGEPAQHRSREEVIRLALEEALVASHRHVFGVLFWYLLLP
                     GPSGAILYRLALYLQRRWRGLGAFGEFTGRAYRVLEWPAVRFTAAAFAVVGDFEDAIY
                     CWRTQAQSWPDPDAGVVLAAGAGAMGVRLGMPLTAIDGVEVRPELGLGEAADSPFLDS
                     VVGLVWRALVVWVFLILLLGLARAV"
     gene            complement(1592..2233)
                     /locus_tag="VFB53_09875"
     CDS             complement(1592..2233)
                     /locus_tag="VFB53_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZN87077.1"
                     /translation="MRATLIDDRPAAPDGDWLRRRFAVGGAPGETLYGDQGADAERAA
                     ARRAAAVLVPVVARPAELTVLLTRRTDHLREHSGQVSFPGGRVEPQDAGAEATALRET
                     REEVGLEPQRVEVLGRLSDYHTRTGFRITPVVGLVSPPFELRLDAHEVAAAFEVPLAF
                     LLDPANHQRRAREYQGRTVHYYAMEWGGHTIWGATAGMLVNLARYLRPAPRAR"
     gene            2321..2560
                     /locus_tag="VFB53_09880"
     CDS             2321..2560
                     /locus_tag="VFB53_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87078.1"
                     /translation="MKKIFLMTTLALFSSAALAGSCPKLMKEIDAALPKAKVSASQMA
                     EVKKLRADGEASHKAGKHADSEASLKKAKQILGLK"
     gene            complement(2609..3580)
                     /locus_tag="VFB53_09885"
     CDS             complement(2609..3580)
                     /locus_tag="VFB53_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZN87079.1"
                     /translation="MTKAVRFHKTGGPEVLQLEEVQVGEPGQGQARIRHTAIGVNFID
                     TYQRSGLYPMQLPATAGNEGAGVVEAVGPGVTQVKAGDRVAYTGQVGSYSGQRLLPAD
                     RLVKIPEGISDEQAASMMLKGMTVQYLIHRTYAVKAGDTVLWHAAAGGVGLIACQWLK
                     ALGATVIGTVGSADKAKLAKSHGADHVINYSTENFVERVKAITGGKGLPVVYDSVGKT
                     TWEGSLDCLRPLGMAVYFGNASGPVPPLNTGILAQKGSLYVTRPTLMTYTASRADLEM
                     TSKSLFDVVKSGKVKIEISARYKLADAAQCHRDLEGRKTTGSVILLP"
     gene            complement(3608..3982)
                     /locus_tag="VFB53_09890"
     CDS             complement(3608..3982)
                     /locus_tag="VFB53_09890"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HZN87080.1"
                     /translation="MAESDAPPQDPFEVFRRLWGPFGVPVPGLAVPTMDPREVEKRVA
                     DLKSVEAWLAMNLNMVRFAIQGLELQRAALQAMQGGAGDAAAGAGPAAGTMLWPWALM
                     QQAMQGQAPAAAEPAAPEKPKE"
     gene            complement(3990..>4351)
                     /locus_tag="VFB53_09895"
     CDS             complement(3990..>4351)
                     /locus_tag="VFB53_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN87081.1"
                     /translation="NSIFLDSLMVDIERLERINRTVQLIPRDKLEDSNILLRPVRVLF
                     ITPSQPIERLAARFIHDLPRSVRFILRPTGALARSGSNLASYLLFEESFCRALIDLGY
                     QDTMAREAEVKEFFADQ"
BASE COUNT          651 a         1581 c         1513 g          606 t
ORIGIN      
        1 tgctctgggt gctcggccgc ctgatcgacc agccctggtt ctacgccggg ctcaacgccg
       61 gcgacccggc ggcggccaag ggtctgctgc tgttcgtgct ggtgatcccc gcgttcacct
      121 tcttcctgca gccgctcgcc agcctctact cgcgccggca cgagttcgag gccgacgcct
      181 acgccgccgc gcacgccagc gccgcggagc tggtgcgcgc gctggtgaag ctctaccagg
      241 acaacgccgc caccctcacc cccgacccgc tgcactcggc gttctacgac tcgcacccgc
      301 ccgccgcgac gcgcatcgcg cgcctgcagg ccggcaccta ggaggcgcca tggaaacgct
      361 cgccaagcgc aagtgcaagc cctgcgaggg cggcgtgccg ccctacagcc cgcagcaggc
      421 gcgcgaactg ctgcagcagc tgaagggctg gatcctcgag gaaggcaagc tggtgaaggt
      481 cttcgcgttc gccaactacc accacaccat ggcgttcgtg aacgcgctcg cctgggtgtc
      541 gcaccgcgag gaccaccacc cggacctcga ggtgggctac aacaagtgcc gcgtcgccta
      601 ctggacgcac gcggtggggg gcctgtcgga gaacgacttc atctgcgccg cgaagtgcga
      661 cgcgctgttc gagctgtgag gcgccgcgcc tagacggcgc gcgccaggcc gagcagcagg
      721 atcaggaaca cccacaccac cagcgcgcgc cacaccagcc cgaccacgct gtcgaggaac
      781 gggctgtcgg cggcctcgcc cagccccagc tcgggccgca cctcgacgcc gtcgatggcg
      841 gtgagcggca tgcccaggcg cacgcccatc gcccccgcgc cggcggcgag caccacgccc
      901 gcgtcgggat cgggccagga ctgcgcctgg gtgcgccagc agtagatcgc gtcctcgaaa
      961 tcgcccacca ccgcgaaggc ggcggcggtg aagcgcaccg cgggccattc cagcacgcgg
     1021 tacgcgcgcc cggtgaactc gccgaaggcg cccagcccgc gccagcgccg ctgcaggtac
     1081 agcgcgaggc ggtacaggat cgccccgctc ggcccgggca gcagcaggta ccagaacagc
     1141 acgccgaaca cgtgccggtg cgaggccacc agcgcctcct cgagggcgag gcggatcacc
     1201 tcctcgcggc tgcggtgctg cgcgggctcg ccgcgccagg cgccgatcag ctcgcgcgcg
     1261 cggtcgacgt cgccggcgcg gatcgccagc tgcaggtcgg tgaagtagtg gctgaactgg
     1321 cggaagccca gcgtcaggta gagcgcggcg acgttgaagg cgatggccag cagcgggctc
     1381 gcccacagca gcaggtagtg cagggcgacg gccgcggcga gcgccggcac caccgccacc
     1441 agccaggcga tcatgccgtg gcgcgcggcg ccggcgttga acgagcgctc cagccagttg
     1501 gcccagcgcg ccagcagggc gaacaccgtc ttgcgctcgc ccagcgggcg ccactgctcg
     1561 agcagcagcg cggcgatgat ggcgatggcg ctcatcgcgc gcggggcgca gggcgcaggt
     1621 accgggcgag gttcaccagc atcccggcag tggcgcccca gatggtgtgc ccgccccatt
     1681 ccatcgcgta gtagtgcacg gtgcggccct ggtattcgcg cgcgcggcgc tggtggttgg
     1741 ccgggtcgag aaggaaggcg agcggcacct cgaaggcggc ggcgacctcg tgcgcgtcca
     1801 ggcgcagctc gaacggcggc gacaccaggc ccaccaccgg cgtgatgcgg aagccggtgc
     1861 gcgtgtggta gtcggagagc ctgcccagca cctcgacgcg ctgcggctcc aggcccactt
     1921 cctcgcgcgt ctcgcgcagc gcggtggcct cggcgccggc gtcctgcggc tcgacgcgcc
     1981 cgccgggaaa gctgacctgc ccggaatgct cgcgcaggtg gtcggtgcgg cgcgtgagca
     2041 gcacggtgag ctccgcgggc cgcgccacca ccggcaccag caccgcggcc gcgcggcgcg
     2101 ccgcggcgcg ctcggcgtcg gcgccctggt cgccgtacag ggtctccccg ggcgcgccgc
     2161 ccacggcgaa ccggcggcgc agccagtccc cgtccggcgc ggccggcctg tcgtcaatca
     2221 gcgtcgcccg catgggaatg tcgagccttc ggcacctgtt aacgttccag ccgggatgat
     2281 aatctgcccc ggtagtcaaa cccgacgaca gaggagaccc atgaagaaaa ttttcctgat
     2341 gaccaccctg gcgctgttca gttccgcggc gctggcgggg agctgcccga agctgatgaa
     2401 ggagatcgac gccgccctgc cgaaggccaa ggtgagcgcc tcgcagatgg cggaagtgaa
     2461 gaagctgcgc gccgacggcg aagcgtccca caaggccggc aagcacgccg attccgaggc
     2521 ctcgctgaag aaggccaagc agatcctcgg cctgaagtaa gagaaacgcc gcccgcgggc
     2581 ggcgtttttg cattcccccg ccggcggctc agggcagcag gatcaccgag ccggtggtct
     2641 tgcgcccctc caggtcgcgg tggcactgcg ccgcgtcggc gagcttgtag cgcgcgctga
     2701 tctcgatctt caccttgccg gacttcacca cgtcgaacag cgacttggag gtcatctcca
     2761 ggtcggcgcg cgacgcggtg taggtcatca gcgtggggcg cgtcacgtac agcgagccct
     2821 tctgcgccag gatgccggtg ttgagcggcg gcaccgggcc cgaggcgttg ccgaagtaca
     2881 ccgccatgcc gagcggccgc aggcagtcca gcgacccttc ccaggtggtc ttgcccaccg
     2941 agtcgtagac caccggcagg cccttgccgc cggtgatcgc cttcacgcgc tcgacgaaat
     3001 tctccgtgct gtagttgatc acgtggtcgg cgccgtgcga cttggcgagc ttcgccttgt
     3061 ccgccgagcc gacggtgccg atgaccgtgg cgccgagcgc cttcagccac tggcaggcga
     3121 tgagccccac gccgcccgcg gcggcgtgcc acagcaccgt gtcgcccgcc ttcaccgcgt
     3181 aggtgcggtg gatcaggtac tgcacggtca tgcccttgag catcatcgag gcggcctgct
     3241 cgtcgctgat gccctcgggg atcttcacca gccggtcggc cggcagcagg cgctggcccg
     3301 agtacgagcc gacctggccg gtataggcga cgcggtcgcc cgccttcacc tgcgtgacgc
     3361 cggggccgac cgcctcgacc acgccggcgc cctcgttgcc cgcggtggcg ggcagctgca
     3421 tcgggtacag gcccgagcgc tggtaggtgt cgatgaagtt gacgccgatc gccgtgtggc
     3481 ggatgcgcgc ctggccctga cccggctcgc ccacctgcac ctcttccagc tgcagcactt
     3541 cggggccgcc ggtcttgtgg aatctcactg ccttggtcat ttcagtctgc tcctcgttgg
     3601 atggcgttca ttccttcggc ttctcgggcg cggccggctc cgcggcggcc ggagcctgcc
     3661 cctgcatcgc ctgctgcatc agcgcccagg gccacagcat tgtaccggca gccggaccgg
     3721 cgcccgcggc ggcgtcgccc gcgccgccct gcatcgcctg cagcgccgcg cgctgcagct
     3781 ccagcccctg gatggcgaag cgcaccatgt tcaggttcat cgccagccag gcctccaccg
     3841 acttcaggtc ggcgacgcgc ttttccacct ccctcgggtc catggtcggc accgccaggc
     3901 cgggcaccgg cacgccgaac gggccccaca ggcggcggaa cacctcgaag ggatcttgcg
     3961 gcggcgcgtc ggattcggcc atcgggccct cactggtcgg cgaagaattc cttcacttcg
     4021 gcctcgcgcg ccatggtgtc ctggtacccc aggtcgatga gcgcgcggca gaacgactcc
     4081 tcgaacagca ggtagctcgc caggttggag cccgagcgcg ccagcgcgcc ggtgggccgc
     4141 aggatgaagc gcacgctgcg cggcaggtcg tggatgaagc gcgcggcgag ccgctcgatc
     4201 ggctgcgacg gggtgatgaa cagcaccctc accgggcgca gcaggatgtt ggagtcctcc
     4261 agcttgtcgc gcgggatcag ctgcacggtg cggttgatgc gctccaggcg ctcgatgtcc
     4321 accatcaggc tgtcgaggaa gatggaattg a
//
LOCUS       DASRIE010000234        19930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1009541, whole genome shotgun sequence.
ACCESSION   DASRIE010000234 DASRIE010000000
VERSION     DASRIE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009541"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFB53_09900"
     CDS             complement(<1..334)
                     /locus_tag="VFB53_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZN87082.1"
                     /translation="MLLRLSVVAVCMFGFGYALVPFYDAICAALGVNSLVQQDSASPA
                     NTQIDASRTVTVELDANAHNLPWNFRPLVRHITVHPGELATVEYEIANVRSAPVTAQA
                     VPSYGPARA"
     gene            complement(373..474)
                     /locus_tag="VFB53_09905"
     CDS             complement(373..474)
                     /locus_tag="VFB53_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HZN87083.1"
                     /translation="MALSRNAKTGLILASIAAAWFFGLILKYWVMGG"
     gene            complement(477..2081)
                     /gene="ctaD"
                     /locus_tag="VFB53_09910"
     CDS             complement(477..2081)
                     /gene="ctaD"
                     /locus_tag="VFB53_09910"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZN87084.1"
                     /translation="MSSAAADTHAHEHGHGHDAHHGQYGGLMRWVTTTNHKDIGTMYL
                     WFSFIMFMVGGAMAMVIRAELFSPGLQIVDPDFFNQMTTMHGLIMVFGAIMPAFVGFA
                     NWLIPMQIGAPDMAFARMNNWSFWILPFAGALLIISFFTPGGAPGVGWTLYAPLTVQM
                     GVGMDLTIFAVHLLGMSSIMGSINIVTTILNMRAPGMTLMRMPLFCWTWLITAYLLIA
                     VMPVLAGAVTMTLTDRHFGTSFFNAAGGGDPVLYQHIFWFFGHPEVYIIALPAFGVVS
                     QVIPAFSRKPLFGYASMVYATSAIAILSFIVWAHHMYTVGMPVTGQLFFMYATTLIAV
                     PTGVKVFNWVATMWKGSLSFETPMLFALGFLFLFTLGGFTGLVLSLVPIDIQLHDTYY
                     VVAHFHYVMVAGALFAAFAGVYFWLPKWTGRMYDEKLGQIHFWLTMIFFNMIFFVQHF
                     LGLAGMPRRIPDYPLMFETWNKISSIGAFGFGLSQLLFLYVVLKCIRHGPKAAAKPWE
                     GADSLEWTHLPTPVPYHTFDPQPDLR"
     gene            complement(2084..3211)
                     /gene="coxB"
                     /locus_tag="VFB53_09915"
     CDS             complement(2084..3211)
                     /gene="coxB"
                     /locus_tag="VFB53_09915"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZN87085.1"
                     /translation="MLKKLGLGVLLLAFCGLAGALEWNLQPAASRLAADIHWLHEAIM
                     VLVIVLFVGVFGFMFWACYAHRKSIGHPASQFHENTLVEVLWTVIPAIILVAIAWPVT
                     KVVIAQKDTSNPDLTVKVTGMQWKWGYDYLKGEGEGISFVSTLSTPREQIEGKAPKGP
                     HYLLEVDNEMVVPVGKKVRVLTTAADVVHSWWVPAFGAKQDAVPGFIRDLWFKAEQTG
                     TFRSQCVELCGKDHGFMPIVVRVVSAEDYSKWVGAQKAQAAKSADDETKAYTLDQLKS
                     RGEKVYAANCVACHQGSGKGLPPAFPALDGSKVVLGPKNAQIDTVLNGVVKGGKPTAM
                     AAFAKQLNNVELAAVITFTRNNWGNKTGEVIQPADVKARRK"
     gene            complement(3229..3675)
                     /locus_tag="VFB53_09920"
     CDS             complement(3229..3675)
                     /locus_tag="VFB53_09920"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HZN87086.1"
                     /translation="MAFSTTPAGFRLSLKRNCSISPRGLLLVFAALAALAMIIASAFA
                     ALGAWLILPFAGLEVLLLAAAFWLTARHATDYERIELERGRLRIEVCEAERLERFEMD
                     ARAARVRMVDGRVLLHAPQAQLEVGRHLDAAARNDLAAELGKRLQS"
     gene            complement(3768..4595)
                     /locus_tag="VFB53_09925"
     CDS             complement(3768..4595)
                     /locus_tag="VFB53_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN87087.1"
                     /translation="MAEIDARAARRAFSRAAPTYLAAARLETEVGKRMLERLDYVRVA
                     PARILDAGCGPAPQAAALRRRYRGSRLVALDFALPMLARARAAAGWAARLRRAAPLVV
                     CAALERLPLAAGSVQLVWSNMALHWAEDAGAALAEFRRVLAPEGLLMLSTLGPDTLKE
                     LRAAAGAARVHRFADMHDLGDALLAAGFSAPVMDMEQLTLRYADAGALLADLRASGQT
                     LARTDRPRGLAGRGLRAALERSGSSATFEVVYGHAWKGTPRKTADGRSIVQVNRLSR"
     gene            complement(4588..5934)
                     /locus_tag="VFB53_09930"
     CDS             complement(4588..5934)
                     /locus_tag="VFB53_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN87088.1"
                     /translation="MWTVAQLQRDLAAGKTTSRELLEQALARIADPAGEGARAFLKVY
                     AEQARAEAAHSDLLRRAGIVRSALEGLPVSVKDLFDVGGDVTRAGSKVLARAAPAAAD
                     APAVARLRAAGAVLVGRTNMVEFAFGTTGLNPHYGTPKNPWDRKAARVPGGSSSGAAV
                     AQADGMAVLSLGSDTRGSIRQPAALCGVVGWKPTQARVPREGAFPLSYTLDTVGPLAN
                     SVACCAAADPILAGEAQAAFTALPVKGLRLLLPRGSLLEDLDAEVGKAFEAALKKLSA
                     AGAAITELPVPAFDRQAEYFKSGGYAGAEAYAIHRRWQDRLAEYDPRVAKRVLFGKDI
                     SAADYVDLGLMRAEYMSTVAALAAPYDAMLMPTTPTVAPTIAEVDADDESYFRWNFRI
                     LRNVGIVNFLDGCALSLPCHAPGTAPVGLSVFGVAMSDRRVLAAGAAVEGVLRARG"
     gene            5963..6673
                     /locus_tag="VFB53_09935"
     CDS             5963..6673
                     /locus_tag="VFB53_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZN87089.1"
                     /translation="MLGKLARRFVKMADLAFGGSCYLCRGAAEAPLCRDCDAELPRLP
                     ALLCPRCALDSGAGEVCGRCVALPPAFDATVAALAYEFPADVLVQALKFRGELALAPL
                     LAGLLAARLPRGVRVDFLLPVPLSAARLRARGFNQALEIARHVAAAAGCTLAPQLAER
                     GRDTPPQAELALGERARNVRGAFRCPRPLGGAAVAVLDDVMTTGATLEELAATLKRAG
                     ASRVVNWVVARTAPPADA"
     gene            6666..7130
                     /locus_tag="VFB53_09940"
     CDS             6666..7130
                     /locus_tag="VFB53_09940"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZN87090.1"
                     /translation="MLEVILYRPEIPPNAGNAIRLAANTGARLHLVEPLGFSLEDRQL
                     KRAGLDYHALAEVRVHRDWAACREALAATRLFAVSTRGALGYADARYANGDGFVFGPE
                     TRGLPAAVLEEFAPDRRLRLPMRPGNRSLNLSNAVAVVVFEAWRQLGFSGAG"
     gene            complement(7057..8100)
                     /locus_tag="VFB53_09945"
     CDS             complement(7057..8100)
                     /locus_tag="VFB53_09945"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZN87091.1"
                     /translation="MKRIAVLGAGAWGTALAAALGARHAVTLWARDAAQAEVIARERS
                     NRRYLPEVELPGQVAVTARWPGDADLLIAATPVAGLREVAARAAGTPLVWLCKGFEAG
                     SGLLPHEIVAQLRAPRAACGALSGPSFALEVARGLPCALTLASAHPAFASAAARRLHG
                     GRLRVYTSPDLVGVEIGGAVKNVMAIAVGIADGLSLGQNARAALITRGLAEITRLGVA
                     LGGKAQTFMGLAGAGDLILTATGDLSRNRRVGLELARGRPLAEILGALGHVAEGVHSA
                     RETLRRATRARVEMPITQAVCAVLEGRLAPPQAVERLLARDATAEARVSPRRKSRAAA
                     RPRTPPRRRRSKD"
     gene            complement(8097..8531)
                     /locus_tag="VFB53_09950"
     CDS             complement(8097..8531)
                     /locus_tag="VFB53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZN87092.1"
                     /translation="MRRLAAAACLLLAAAPAQAQFLSVGENAAVLYDAPSRQAKALYV
                     VSKHYPVEVIVNLEAWVKVRDHTGALSWVERRLLVEQRTVVVVPPSAEVRVRPEDGAP
                     VAFVAVQNVALELLGTAPGGWLRVRHADGADGYLRANLVWGA"
     gene            complement(8538..9002)
                     /gene="secB"
                     /locus_tag="VFB53_09955"
     CDS             complement(8538..9002)
                     /gene="secB"
                     /locus_tag="VFB53_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZN87093.1"
                     /translation="MADAQQPSFQIEKIYVKDVSLEIPNAPQVFLEAQGPQLEIQVRN
                     EAQQFAEGLFEVVVTVTVTAKAGEKTLFLAEAAQAGIFAVRGVPPAEIEPVLAVACPT
                     ILYPYAREAISDLVTRGGFPPVLLAPVSFEAIYAQRAQQRQAADGPRIEVAN"
     gene            complement(9015..9281)
                     /gene="grxC"
                     /locus_tag="VFB53_09960"
     CDS             complement(9015..9281)
                     /gene="grxC"
                     /locus_tag="VFB53_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZN87094.1"
                     /translation="MAQVLMYLTAACPYCQAAQRLLEKKGIRDVERVRVDLEPARREE
                     MTRRSGRYTVPQIWVGERHVGGCDELYALERDGTLDRLLETEKT"
     gene            complement(9283..9690)
                     /locus_tag="VFB53_09965"
     CDS             complement(9283..9690)
                     /locus_tag="VFB53_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN87095.1"
                     /translation="MEFVQNNILLIAVAFASGAMLVWPYVRRSTGGPWVSTLQATQMI
                     NRQDALLLDVRDAAEYAKGHILGAKNVPVADLERRAAEFDKHRAKPVIVHCDTGNRAT
                     RAAGVLKARGFSSVFNLAGGFGAWQQAGLPVEK"
     gene            9718..10467
                     /gene="gpmA"
                     /locus_tag="VFB53_09970"
     CDS             9718..10467
                     /gene="gpmA"
                     /locus_tag="VFB53_09970"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HZN87096.1"
                     /translation="MHRIVLLRHGESAWNRENRFTGWTDVDLSPAGVEEARAAGRLLR
                     AEGYAFDLAHTSVLKRAIRTLWLSLEEMDCLWLPVEKHWRLNERHYGALQGLNKAEMA
                     AKFGEAQVLAWRRSYDTPPPALEPGDARDAAADPRYAGLARAEVPLTECLQDTVARVL
                     PYWNAAIAPAVRAGRRMLVVAHGNSLRALIKHLDGVSDADIVGLNVPTGVPLVYELDA
                     QLRPLRHHYLGDAAEIARRVAAVSAQGKARP"
     gene            10545..11633
                     /locus_tag="VFB53_09975"
     CDS             10545..11633
                     /locus_tag="VFB53_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZN87097.1"
                     /translation="MRSRIEALRSGLGEKEADRREARDALRDSERAISDANRGLRRLE
                     AEGRAAQAELARLGARQKALAASLARQQAVLGRVLAARYAAGGRQASDALKVALSGES
                     PSDVARRLHYLAELSRATAGLLRELRAGLADAAEVRRAAEERRSRLAAIEREQRADRD
                     RVLAERRERRRVLERVSVEIRKARRQVQTLQADERRLARLVEEIARVLTDRPGAGYAR
                     VEKVPVLGSLAGPFSTLRGKLRLPVLGELVGRFGAPRNMGPAGKNGVFIRAQEGQAVR
                     AVAAGQVVYADWMRGFGNLLIIDHGEAYLSIYANNESLLKQTGDPVAAGETVATVGAS
                     GGNEQSGLYFELRHLGRAFDPLRWVVLK"
     gene            11650..13047
                     /locus_tag="VFB53_09980"
     CDS             11650..13047
                     /locus_tag="VFB53_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZN87098.1"
                     /translation="MGKFRNLGLIALGAVAGVLVSLNFQAIADRASRGPLPIEELRAF
                     TEVFGAIKQNYVETVEDKKLITEAINGMLSGLDPHSAYLDAEAFKELQVGTQGQFGGL
                     GIEVGMEDGFVKVVSPIEDTPAFRAGIKPGDLIIRLDETPVKGMALNDAVKRMRGKPN
                     TQITLTISRKGEAAPIVVTLTRAVIRVQSVKSKILEPGYGWVRVSQFQESTTENLVRH
                     LDTLYKQGQLKGLVLDLRNDPGGLLYGAVAISSAFLPAKALVVSTDGRTEDAKKKFHA
                     SPEDYSRGVRDDVLRPLPAAVKNVKLVVLVNGGSASASEIVAGALQDHKRATVIGTQT
                     FGKGSVQTIMPLGNSTAIKLTTARYYTPSGRSIQAKGITPDIVVEEPGASPQSRVRES
                     DLERHLSNDRDKEGAKKPGAGAKPSAVPPGELPKPIEMGSKEDFQLAQALNFLKGAPV
                     KGQPTKAVAETKSGS"
     gene            13095..13790
                     /gene="moeB"
                     /locus_tag="VFB53_09985"
     CDS             13095..13790
                     /gene="moeB"
                     /locus_tag="VFB53_09985"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZN87099.1"
                     /translation="MGIEGQERLRAASALVVGAGGLGCPAALYLASGGIGRLTIADAD
                     SVDLTNLQRQILYRTDSVGAKKVDEAKKALGAVNPEVEVVALARRVDGSLVEELVAAA
                     DVVLDCSDNFATRHALNRACVQHRKPLVSGAAIRFDGQLMVFDLRSKDAPCYACLFPE
                     DGEVEEVQCSVMGVVAPLTGAIGAMQALEAIKLLAGAGETPLGRLTLIDARASETRSV
                     RVKKDPACAVCAG"
     gene            13801..14487
                     /locus_tag="VFB53_09990"
     CDS             13801..14487
                     /locus_tag="VFB53_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZN87100.1"
                     /translation="MSFSPAGWRRGFLRAQPLAVGVFGYAVAFGLLASEAGLSVLEAA
                     LMSATVYSGSAQVATVGGLAAGAGILASVVTVLMLNARYLLYGASLRPWLSQSTPGQA
                     YASLYFLGDGNWLLSMNARNAGEQDAGFVFGSGIAMFLPWVVGTLLGSVAGSWLGRPE
                     LLALDFLLVAFCAAMATDLFKSRGDLWTVAVALAVALGVERLAPSGWTIVATGLAGIV
                     AGYFRHEERA"
     gene            14484..14792
                     /locus_tag="VFB53_09995"
     CDS             14484..14792
                     /locus_tag="VFB53_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZN87101.1"
                     /translation="MTLRLEILPLIFALALASFACRAGGFWIMRFVRMTPRIQSALRA
                     APLAVMVGIVAPAAARGHPPELLGIAVTAAVMRLTGSDLGAALAGVATVALGRALLRG
                     "
     gene            14806..16155
                     /locus_tag="VFB53_10000"
     CDS             14806..16155
                     /locus_tag="VFB53_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN87102.1"
                     /translation="MNAPAAADRQHCDVLVVGGGPAGSTTAAVLAEKGWRVTLLEKER
                     HPRFHIGESLLPHNLPIFKRLGVLEQVDAIGIKKPGVDFTAELEVSGYHEFKFAFALD
                     TSQPYAYEVRRSEFDNLLLRNSAAKGAQVIEGLRVTRVDFPEGGDPVVQAVDAEGREC
                     SWSCRYFVDATGRDTLLSKKFDLKRKNKHHASAAIFGHFLDVDRRAGDDAGNISLYWF
                     QHGWFWMIPLKDGSMSVGAVCRPEYLKQRRNSPPEFLMQTIALAPAEMRHRMRKATLM
                     DNMARATGNYSYQSTRMRGERYVMVGDAFAFIDPVFSSGVLLGMNSGMLGAEAVDVWL
                     REGAAAAESRFRHFERMVLRGVETFSWFIWRFNSPGMRFLMLQPGNPFRVQEAVISLL
                     AGDVFRDIGARSRFVLFKLFYFLFSLRHPRESFRLWLKRIRNPSVAFTGGTTPLDER"
     gene            complement(16171..17370)
                     /locus_tag="VFB53_10005"
     CDS             complement(16171..17370)
                     /locus_tag="VFB53_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN87103.1"
                     /translation="MSTMEVFLIALGIIFTVPYLIWRLGRTDYYAPLVVVQVAAGILL
                     GPGILGKVFPDYYAFVFTEPVVQSLNGIAWWAVMVFVWIAGIELDLRKAWVRRRESAI
                     TAGLALATPLLAGGGVAAVMLSYGGWIGPAALPWQFVLGVGMACAVTALPVLLLLMDR
                     LEILRQPLGQRILRYASLDDVAIWGVLALILMDWQRVGLQLVFLAAFGISAYGFRRLM
                     LRLPPGDRWYLGLVWLTVCAFGADWCGLHFMVGAFLAGAVMDGDWFDREQLDLLRHHV
                     LLIAMPVFFLSTGLRTNWSVGGEAVFLAAAALLFASVGGKLLGVRAAGKILRWPAGEA
                     PVLGWLLQTKGLIMIIFANVLMDKGVITSETFTALLLMALGSTMLAVPVVAPKLSRLP
                     GMVTRAD"
     gene            complement(17367..18056)
                     /locus_tag="VFB53_10010"
     CDS             complement(17367..18056)
                     /locus_tag="VFB53_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87104.1"
                     /translation="MPSPPETGDDGAELQQYVARKSAHMHARAMYRRLKATVDGWRHE
                     ERAKARVAVESIVWFLLVMSAAIGFGHLTGIGALGYLAIGFMVWFAVVLAVMAEQLAG
                     KPGELAKPRRLADRALLGLAIAADVLGLLTTWPLFAMHPSLLSIGRGVVLLALFGALL
                     ALLTRPQMFGARVIPVSIALCAAVPLATLAGALDLGAVSGLEVYAIIVAAAIGWLNWV
                     VIRRQGAHRAP"
     gene            complement(18085..18639)
                     /gene="slmA"
                     /locus_tag="VFB53_10015"
     CDS             complement(18085..18639)
                     /gene="slmA"
                     /locus_tag="VFB53_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HZN87105.1"
                     /translation="MPRVKGERRLEILKALAQMLEAPRWERITTAALAAKLDVSEAAL
                     YRHFASKAQMYEGLIEFIESSVFSVANRITADEPDGRKQADQLLEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARMNQFYDRFEATLKQALRVSSDGDAAGRASVLLRYAIGSLH
                     QFAKSGFAKKPSEGYAAQRGYLSA"
     gene            complement(18652..19317)
                     /locus_tag="VFB53_10020"
     CDS             complement(18652..19317)
                     /locus_tag="VFB53_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HZN87106.1"
                     /translation="MRLVGLPPARRVWFFDLDNTLHDAQARIFPAMHDQINAYLMRRF
                     AVDEAGANAMRRGFWEKYGTTMEGLARHHGASRAEFLWATHQFPELADMVVHENAVRH
                     ALARLPGRKILYSNAPRHYVDAVVRILGVARYFDAVYSIESSRYRGKPSLSGFRLLLR
                     RHHLEAARCALVDDMLENLRTAKRLGMATVWVSRERKNVPYVDLRVASVTQLPGLVFR
                     YSH"
     gene            complement(19322..>19930)
                     /gene="argB"
                     /locus_tag="VFB53_10025"
     CDS             complement(19322..>19930)
                     /gene="argB"
                     /locus_tag="VFB53_10025"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZN87107.1"
                     /translation="TDRETMDVVEMVLGGQVNKEVVELINQAGGKAVGLTGQDGAFIR
                     ARKMLLPSRENGKVDIGQVGDIESIDPSVIGALEQGGFIPVVAPIATGADGTTYNINA
                     DLVAGKLAEVLRAEKLVVLTNTPGVLDKDGKLLTGLTPRRIDDLVAKGVISGGMLPKI
                     GSALDAARNGVKSVHIIDGRVPNALLLEVMTDQGVGTLIKSK"
BASE COUNT         3005 a         7078 c         7013 g         2834 t
ORIGIN      
        1 cggcgcgcgc cgggccgtag ctcggcaccg cctgcgccgt caccggcgcg ctgcgcacgt
       61 tggcgatctc gtactccacc gtggccagct cgccggggtg cacggtgatg tggcgcacca
      121 gcggcctgaa gttccacggc aggttgtgcg cgttggcgtc cagctcgacg gtcaccgtgc
      181 gcgaggcgtc gatctgcgtg ttggccggcg acgcgctgtc ctgctgcacc agcgaattga
      241 cgcccagggc cgcgcagatc gcgtcgtaga acggcaccag cgcgtagccg aagccgaaca
      301 tgcagaccgc gaccacgctc aggcgcagga gcatgcggcg gttttcccgc gcgcgcgggg
      361 ccgcggcggc gctcatccgc ccatcaccca gtacttgagg atcaggccga agaaccacgc
      421 cgccgcgatc gaggcgagga tcaacccggt cttcgcgttg cggctcagcg ccatgctcat
      481 cgaagatcgg gttgcgggtc gaaggtgtgg tagggcaccg gcgtcggcag gtgcgtccac
      541 tccagcgagt cggccccctc ccagggcttc gccgccgcct tcgggccgtg cctgatgcac
      601 ttgagcacga cgtacaggaa cagcagctgc gacaggccga agccgaaggc gccgatcgag
      661 ctgatcttgt tccaggtctc gaacatcagc ggatagtccg ggatgcgccg cggcatgccg
      721 gcgaggccga ggaaatgctg cacgaagaag atcatgttga agaaaatcat cgtcagccag
      781 aagtggatct ggccgagctt ctcgtcgtac atcctgccgg tccatttcgg cagccagaaa
      841 tacacgccgg cgaaggccgc gaacagggcg cccgccacca tcacgtagtg gaaatgcgcc
      901 accacgtagt aggtatcgtg cagctggatg tcgatcggca ccagcgacag caccaggccg
      961 gtgaagccgc ccagcgtgaa caggaacagg aacccgagcg cgaacagcat cggcgtctcg
     1021 aagctgagcg agcctttcca catggtcgcc acccagttga acaccttgac gccggtgggc
     1081 accgcgatca gcgtcgtcgc gtacatgaag aacagctgcc cggtgaccgg catgcccacc
     1141 gtgtacatgt ggtgcgccca cacgatgaac gacaggatcg cgatcgccga ggtggcgtag
     1201 accatcgagg cgtatccgaa caggggcttt ctcgagaacg ccgggatcac ctgcgacacc
     1261 acgccgaagg cgggcagcgc gatgatgtac acctcgggat ggccgaagaa ccagaagatg
     1321 tgctggtaca gcaccgggtc gccgccgccc gcggcgttga agaagctggt gccgaagtgc
     1381 cggtcggtga gcgtcatggt caccgccccg gcgagcaccg gcatcaccgc gatcaggagg
     1441 taggccgtga tcagccaggt ccagcagaac agcggcatgc gcatcagcgt catccccggc
     1501 gcgcgcatgt tgaggatggt ggtgacgatg ttgatcgagc ccatgatcga gctcatgccg
     1561 agcaggtgta ccgcgaagat ggtgaggtcc atgccgacgc ccatctgcac ggtgagcggc
     1621 gcgtagagcg tccagccgac gccgggtgcg ccgccgggcg tgaagaagga gatgatcagc
     1681 agggcgccgg cgaagggcag gatccagaac gaccagttgt tcatgcgcgc gaaggccatg
     1741 tcgggcgcgc cgatctgcat cgggatcagc cagttggcga agccgacgaa ggccggcatg
     1801 atcgcgccga acaccatgat caggccgtgc atggtggtca tctggttgaa gaagtccggg
     1861 tcgacgatct gcagcccggg cgagaacagc tcggcgcgga tcaccatcgc catcgcgccg
     1921 cccaccatga acatgatgaa gctgaaccac aggtacatgg tgccgatgtc cttgtggttg
     1981 gtggtggtga cccagcgcat caggccgccg tactggccgt ggtgcgcgtc gtgcccgtga
     2041 ccgtgctcgt gggcgtgggt gtctgcggct gcgctgctca tcgctatttc ctccgggcct
     2101 tgacgtcggc gggctggatc acctcgccgg tcttgttgcc ccagttgttg cgcgtgaagg
     2161 tgatcaccgc ggccagctcg acgttgttga gctgcttggc gaaggcggcc atcgcggtcg
     2221 gcttgccgcc tttgacgacg ccgttcagca cggtgtcgat ctgcgcgttt ttcggcccga
     2281 gcaccacctt ggagccgtcg agcgccggga atgcgggcgg caggcccttg ccgctcccct
     2341 ggtggcaggc gacgcagttg gcggcgtaca ccttctcgcc gcgcgacttg agctggtcca
     2401 gcgtgtaggc cttggtctcg tcgtcggcgg acttggccgc ttgcgctttt tgcgcgccga
     2461 cccacttgct gtagtcctcg gcgctcacca cgcgcaccac gatcggcatg aagccgtggt
     2521 ccttgccgca gagctcgacg cactggctgc ggaacgtgcc ggtctgctcg gccttgaacc
     2581 acaggtcgcg gatgaagccg ggcaccgcgt cctgcttggc gccgaaggcc ggcacccacc
     2641 aggagtgcac cacgtccgcg gcggtggtca gcacgcgcac cttcttgccc accggcacca
     2701 ccatctcgtt gtccacctcg agcaggtagt gcggcccttt gggcgccttg ccctcgatct
     2761 gctcgcgcgg cgtggacagc gtggagacga agctgatgcc ctcgccctcc cccttgagat
     2821 agtcgtagcc ccatttccac tgcatccccg tcaccttgac ggtgaggtcg gggttcgaag
     2881 tgtccttctg cgcgatcacc accttggtca ccggccaggc gatggccacc aggatgatcg
     2941 ccgggatcac ggtccacagc acttcgacca gggtgttctc gtggaactgc gaggccgggt
     3001 ggccgatcga cttgcggtgc gcgtagcacg cccagaacat gaagccgaac acgcctacga
     3061 acagcacgat gaccagcacc atgatcgctt cgtgcagcca gtggatgtcg gccgcgagcc
     3121 gcgaggcggc cggctgcaga ttccactcca aggcgcccgc cagcccgcaa aaggccagca
     3181 gcagcacgcc caaccccagc ttcttcagca tccttgcccc ttcccctgtc agctttgcag
     3241 tctcttgccg agctcggcgg ccagatcgtt gcgcgccgcc gcgtccaggt ggcggcccac
     3301 ctccagctgc gcctgcggcg catgcagcag cacgcgcccg tcaaccatgc gcacgcgcgc
     3361 cgcgcgcgcg tccatctcga agcgctccag gcgctcggcc tcgcacactt cgatgcgcag
     3421 ccgcccgcgc tcgagctcga tgcgctcgta gtccgtcgca tgccgcgccg tgagccagaa
     3481 ggcggcggcc agcagcagca cctccagccc ggcgaacggc aggatcagcc aggcccccag
     3541 cgccgcgaag gccgacgcga tgatcatggc gagcgccgcg agcgccgcga acaccagcag
     3601 caggccgcgc ggcgagatcg agcaattgcg cttgagcgag agccggaatc ccgccggcgt
     3661 cgtcgaaaac gccacggcgc gaggcgggaa gtcgaagctc agcgaggtca cgaattgtct
     3721 cggcgcacac agcgaaaaac gaggcgaaac aataacatga atttggacta gcgcgacagc
     3781 cggttcacct gcacgatgct gcggccgtcc gccgtttttc gcggcgtgcc tttccaggcg
     3841 tggccgtata ccacctcaaa ggtggcgctg ctgcccgagc gctcgagcgc tgcgcgcaag
     3901 ccgcggccgg cgaggccgcg cggccggtcg gtgcgcgcga gcgtctggcc gctggcgcgc
     3961 aggtcggcga gcagcgcgcc cgcgtcggcg taccgcaggg tcaactgctc catgtccatc
     4021 accggcgcgg aaaatcccgc cgccagcagc gcgtcgccga ggtcgtgcat gtccgcgaac
     4081 cggtgcacgc gcgccgcgcc ggccgcggcg cgcagctcct tcagggtgtc ggggccgagc
     4141 gtgctgagca tcagcaggcc ctcgggcgcg agcacgcgcc ggaattcggc cagcgcggcg
     4201 cccgcgtcct cggcccagtg cagggccatg ttcgaccaca ccagctgcac gctgcccgcg
     4261 gcgagcggca gccgctccag cgccgcgcac accacgagcg gcgcggcgcg ccgcaggcgc
     4321 gccgcccagc cggccgcggc gcgcgcgcgc gccagcatcg gcagggcaaa gtcgagggcc
     4381 accaggcgcg acccccggta gcgccggcgc agggccgcgg cctgcggcgc cgggccgcag
     4441 ccggcgtcga gaatgcgcgc cggcgccacg cgcacgtagt ccaggcgctc cagcatgcgc
     4501 ttgccgactt cggtttccag ccgcgccgct gcaaggtagg tcggcgcggc gcggctgaaa
     4561 gcgcggcgcg cggcgcgcgc gtcgatctca gccacgcgcg cgcaggacgc cctcgacggc
     4621 agcgcccgcg gcgagcacgc ggcggtcgga catcgcgacg ccgaacacgc tcaggcccac
     4681 cggcgccgtg cccggcgcgt ggcagggcag cgacagcgca cagccgtcga ggaaattcac
     4741 gatcccgacg ttgcgcagga tgcggaagtt ccagcggaag taggactcgt cgtcggcgtc
     4801 cacctcggcg atcgtcggcg cgacggtcgg cgtggtgggc atcagcatgg cgtcgtacgg
     4861 ggcggcgagc gccgccacgg tgctcatgta ctcggcgcgc atcaggccga ggtcgacgta
     4921 gtccgcagcg ctgatgtcct tgccgaacag gacccgcttg gcgacgcgcg ggtcgtactc
     4981 ggcgagccgg tcctgccagc ggcgatggat ggcgtaggcc tcggccccgg cgtagccgcc
     5041 gctcttgaaa tactccgcct gccggtcgaa ggcgggcacc ggcagttcgg tgatcgcggc
     5101 gcccgctgcg gaaagcttct tcagcgccgc ctcgaacgcc ttgccgactt cggcatccag
     5161 gtcttcgagc agcgagccgc gcggcagcag caggcgaagg cccttgacag ggagcgcggt
     5221 gaaagcggcc tgcgcttcgc ccgccaggat cgggtccgcg gcggcgcaac aggccacgct
     5281 gttggcgagc ggccccaccg tatccagggt gtaggacagc gggaacgcgc cctcgcgcgg
     5341 cacgcgcgcc tgcgtcggct tccagcccac gacaccgcac agcgccgccg gctggcggat
     5401 cgagccgcgc gtatcgctgc ccagcgagag cacggccatg ccgtcggcct gcgccaccgc
     5461 ggcccccgag gaggacccgc cgggcacgcg cgcagccttg cggtcccagg gattcttcgg
     5521 cgtgccgtag tgcgggttga ggccggtggt gccgaaggcg aactccacca tgttggtgcg
     5581 gccgacgagc accgcgcccg cggcgcgcag ccgcgccacc gccggcgcat cggccgcggc
     5641 gggagccgcg cgcgccaaca ccttcgagcc ggcgcgcgtc acgtcgccgc cgacgtcgaa
     5701 caggtccttc accgagaccg gcagtccctc cagcgccgag cgcacgatgc ccgcgcgccg
     5761 cagcaggtcc gaatgcgccg cctcggcgcg cgcctgctcg gcatacacct tgaggaaggc
     5821 ccgcgctccc tcgcccgccg ggtccgcgat gcgcgccagc gcctgctcca gcagctcgcg
     5881 gctggtggtc ttgcccgccg ccagatcgcg ctgcaactgc gctaccgtcc acatcggcgc
     5941 agtctagcgc acaataatcg cgatgctcgg caagctcgcg cggcggttcg tgaagatggc
     6001 ggacctggca ttcggcggca gctgctacct gtgccgcggc gccgccgagg ccccgctgtg
     6061 ccgcgactgc gacgccgagc tgccgcggct gcccgcgctg ctgtgcccgc gctgcgcgct
     6121 cgactcgggc gcgggggagg tgtgcggccg ctgcgtggcg ctgccgcccg ccttcgacgc
     6181 taccgtcgcc gccctcgcct acgagtttcc cgcggatgtg ctggtgcagg cgctgaaatt
     6241 ccgcggcgag ctggcgctcg cgcccctgct cgccgggctg ctggcggcgc gcctgccgcg
     6301 cggcgtgcgg gtggatttcc tgctgccggt gccgctgtcg gccgcgcggc tgcgcgcgcg
     6361 cgggttcaac caggcgctgg agatcgcgcg ccacgtggcg gccgcggccg gctgcacgct
     6421 cgcgccgcaa ctcgccgagc gcggccgcga cacgccgccg caggccgagc tcgccctcgg
     6481 cgagcgcgcg cgcaacgtgc gcggcgcgtt tcgctgcccg cgtccgctcg gaggcgccgc
     6541 ggtcgccgtg ctggacgacg tgatgaccac cggcgcgacg ctcgaggagc tcgcggcgac
     6601 gctcaagcgc gcgggcgcct cgcgcgtggt caactgggtg gtggcgcgca ccgccccgcc
     6661 ggccgatgct tgaggtgatc ctgtaccggc cggagatccc gcccaatgcg ggcaacgcga
     6721 tccgcctcgc cgccaatacg ggcgcgcggc tgcacctggt cgagccgctc ggcttctcgc
     6781 tggaagaccg gcagctcaag cgcgcgggcc tcgactacca tgcgctggcc gaggtgcgcg
     6841 tgcatcgcga ctgggcggcc tgccgcgagg cgctcgccgc aacccgcctg ttcgccgtgt
     6901 ccacgcgcgg cgcgctgggc tacgccgacg cgcgctacgc gaacggcgac ggcttcgtgt
     6961 tcgggccgga aacccgcggg ctgccggccg cggtgctgga ggaattcgcc cccgaccggc
     7021 ggctgcgcct gccgatgcgc cccggcaacc gcagcctcaa tctttcgaac gccgtcgccg
     7081 tggtggtgtt cgaggcctgg cggcagctcg gcttttccgg cgcgggctga cgcgcgcttc
     7141 cgccgtcgcg tcgcgcgcca gcagccgctc caccgcctgc ggcggcgcca gtcgtccctc
     7201 gagcaccgcg cacaccgcct gcgtgatcgg catctcgacg cgcgcgcgcg tcgcgcggcg
     7261 cagcgtctcg cgcgccgagt gcacgccttc ggccacgtgg cccagggcgc ccaggatctc
     7321 ggcgaggggc cggccgcgcg ccagctccag gccgacgcgg cggttgcgcg acaggtcgcc
     7381 cgtcgccgtc aggatcaggt cgcccgcgcc cgccaggccc atgaaggtct gcgccttgcc
     7441 gccgagcgcc acgccgaggc gcgtgatctc cgccaggccg cgcgtgatca gcgcggcgcg
     7501 cgcgttctgg ccgagagaca ggccgtcggc gatgccgacc gcgatcgcca tcacgttctt
     7561 caccgcgccg ccgatctcga cgccgaccag gtccgggctg gtgtacacgc gcagccggcc
     7621 gccgtgcagc cggcgcgcgg cggcactcgc gaacgccgga tgcgccgatg cgagcgtcag
     7681 cgcgcagggc agcccgcgcg ccacttccag cgcgaacgag ggcccggaga gcgccccgca
     7741 ggcggcgcgc ggcgcgcgca gctgcgcgac gatttcgtgc ggcagcagcc cgctgccggc
     7801 ctcgaagccc ttgcacagcc acaccagcgg cgtgcccgcg gcgcgcgcgg ccacctcgcg
     7861 cagcccggcc accggggtcg cggcgatcag caggtcggca tcgccgggcc agcgcgcggt
     7921 gactgccact tgacccggca attcgacctc cggcaggtag cggcggttgc tgcgttcgcg
     7981 cgcgatgacc tcggcctgcg cggcatcgcg cgcccacagc gtgacggcat ggcgggcgcc
     8041 gagcgccgcc gcgagcgccg ttccccaggc gcccgcgccg agcacggcga tgcgcttcac
     8101 gccccccaga cgaggttcgc gcgcaggtag ccgtcggcgc cgtcggcatg gcgcacgcgc
     8161 agccagccgc cgggcgcggt gccgagcagc tccagcgcca cgttctgcac cgcgacgaac
     8221 gccaccggcg cgccgtcctc ggggcgcacg cgcacttccg ccgagggcgg caccaccacg
     8281 accgtgcgct gctcgaccag gagcctgcgc tccacccagc tgagcgcgcc ggtgtgatcg
     8341 cgcaccttca cccaggcctc gaggttgacg atcacctcca ccgggtagtg cttcgacacc
     8401 acgtacagcg ccttggcctg gcgcgacggc gcgtcgtaga ggacggcggc attctcgccg
     8461 acgctcagga attgcgcctg cgccggggcg gcggcgagca gcaggcaggc ggcggcagcg
     8521 aggcggcgca tggcgttcta gtttgccact tcgatgcgcg ggccatcggc ggcctgccgc
     8581 tgctgggcac gctgcgcgta gatcgcctcg aaggacaccg gggcaagcag cacgggcgga
     8641 aagccgccgc gcgtcaccag gtcggaaatg gcctcgcgcg cgtagggata gaggatcgtc
     8701 gggcaggcga cggcgagcac cggctcgatc tcggccggcg gcacgccgcg caccgcaaag
     8761 atgccggcct gcgcggcttc ggcgaggaac agcgtcttct cgccggcctt ggcggtgacg
     8821 gtgacggtga ccaccacctc gaacagcccc tcggcgaact gctgcgcctc gttgcgcacc
     8881 tggatctcca gttgcggacc ctgcgcctcc aggaacacct gcggcgcgtt ggggatctcc
     8941 agcgacacgt ccttgacgta gatcttctcg atctggaagc tgggctgctg cgcgtcggcc
     9001 atggcggcgg gttcctaggt cttctcggtc tcgagcaggc ggtccagcgt gccgtcgcgc
     9061 tcgagggcgt acagctcgtc gcaaccgccg acatggcgct cgccgaccca gatctgcggc
     9121 acggtgtaac gccccgagcg ccgcgtcatc tcctcgcgcc gcgccggctc caggtcgacg
     9181 cgcacccgtt ccacgtcgcg gatgcctttc ttctccagca gccgctgcgc cgcctggcag
     9241 taggggcagg cggcggtgag gtacatcagc acctgcgcca agctacttct ccaccggcag
     9301 cccggcctgc tgccaggcgc cgaagccgcc ggcgaggttg aacacgctgg aaaagccgcg
     9361 cgccttgagc acgccggcgg cgcgcgtggc gcggttgccg gtgtcgcaat gcacgatcac
     9421 cggcttcgcc ctgtgcttgt cgaactccgc ggcgcgccgc tccaggtcgg cgaccggcac
     9481 gttcttcgcg ccgaggatgt ggcccttggc gtactcggcg gcgtcgcgca cgtcgagcag
     9541 cagcgcgtcc tggcggttga tcatctgcgt ggcctgcagc gtgctgaccc aggggccgcc
     9601 ggtgctgcgg cgcacgtacg gccagacgag catcgcgccc gaggcgaagg ccacggcgat
     9661 gagcaggatg ttgttctgga cgaattccat cgggagcgcg acattataat ccgccgcatg
     9721 caccgcatcg tgctcctgcg ccacggcgag tcggcctgga accgcgagaa ccgctttacc
     9781 ggctggaccg acgtggacct gtcgccggcc ggcgtcgagg aagcgcgcgc cgccgggcgc
     9841 ctgctcaggg ccgagggcta cgccttcgac ctcgcccaca cctcggtgct gaagcgcgcc
     9901 atccgcaccc tgtggctgtc gctggaggaa atggactgcc tgtggctgcc ggtggaaaag
     9961 cactggcgcc tgaacgagcg ccactacggc gccctgcagg gcctgaacaa ggccgagatg
    10021 gcggcgaagt tcggcgaggc gcaggtgctg gcgtggcggc gcagctacga cacgccgccg
    10081 ccggcgctcg agccgggcga tgcgcgggac gccgccgccg acccgcgcta cgccggcctc
    10141 gcgcgcgccg aggtgccgct caccgagtgc ctgcaggaca cggtggcgcg ggtgctgccc
    10201 tactggaacg cggcgatcgc tccggcggtg cgcgcgggcc ggcgcatgct ggtggtggcg
    10261 cacggcaact cgctgcgcgc gctgatcaag cacctggacg gcgtgtcgga cgccgacatc
    10321 gtcggcctca acgtgcccac cggcgtgccg ctggtgtacg agctggacgc gcagctgcgg
    10381 ccgctgcggc accactatct gggcgacgcc gccgagatcg cgcgccgcgt ggccgcggtc
    10441 tccgcgcaag ggaaggccag gccctgatcc gggcgcttgt cctcggcctg gcgctcgtcg
    10501 cgagcgccgc ggcgcaggcc ggccgcgagg aagacctgaa ggccttgcgc tcgcgcatcg
    10561 aagcgctcag gtccggcctg ggcgagaagg aggccgaccg gcgcgaggcg cgcgacgcgc
    10621 tgcgcgactc cgagcgcgcc atctcggatg ccaaccgcgg cctgcgcagg ctggaggccg
    10681 agggccgcgc ggcgcaggcc gaactcgcgc gcctcggcgc gcggcagaag gcgctggcgg
    10741 cgagcctggc gcgccagcag gccgtgctcg gccgcgtgct cgcggcgcgc tacgccgccg
    10801 gcggacggca ggcgagcgac gcgctcaagg tggcgctctc cggcgagagc ccctcggacg
    10861 tcgcgcgccg gctgcactac ctcgccgagc tctcgcgcgc caccgccggg ttgctgcgtg
    10921 aactgcgcgc cggcctggcc gacgcggccg aggtgcgccg cgcggccgag gagcggcgct
    10981 cccggctggc ggccatcgag cgcgagcagc gcgccgatcg cgaccgcgtt ctcgccgagc
    11041 ggcgcgagcg gcggcgggtc ctcgagcgcg tgtcggtcga gatccgcaag gcgcgccggc
    11101 aggtgcagac cctgcaggcg gacgagcggc gcctggcgcg cctcgtcgag gagatcgcgc
    11161 gcgtgctcac cgaccgcccg ggcgccggct acgcgcgcgt ggaaaaagtc ccggtactgg
    11221 gctccctggc cgggccgttc tccacgctgc gcggcaagct gcggctgccc gtgctggggg
    11281 aactcgtcgg gcgcttcggc gcaccaagga acatgggccc ggcgggcaag aacggcgtgt
    11341 tcatccgcgc ccaggaaggc caggcggtgc gcgcggtcgc cgcggggcag gtggtgtacg
    11401 cggactggat gcgcggcttc ggcaacctgc tgatcatcga ccatggcgag gcctacctgt
    11461 ccatctacgc caacaacgag tcgctgctca agcagacggg ggatccggtc gccgcgggcg
    11521 agacggtggc cacggtcggg gcgagcggtg gcaacgagca atcgggctta tactttgaac
    11581 tcaggcacct gggcagggca ttcgatccgc tgcgctgggt agtgctgaaa taaggagccg
    11641 tccgacacca tgggcaagtt caggaacctc gggctgatcg cgctcggcgc ggtcgccggc
    11701 gtgctggtca gcctcaactt ccaggcgatc gccgaccgcg cctcgcgcgg cccgctgccg
    11761 atcgaggagc tgcgcgcctt taccgaggta ttcggcgcga tcaagcagaa ttacgtcgag
    11821 acggtcgaag acaagaagct catcaccgag gcgatcaacg gcatgctctc ggggctcgat
    11881 ccgcattcgg cctacctgga cgccgaggcc ttcaaggagc tccaggtcgg cacccagggg
    11941 cagttcggcg gcctgggcat cgaggtcggg atggaggacg gcttcgtcaa ggtggtctcg
    12001 ccgatcgagg acacgcccgc gttccgcgcc ggcatcaagc ccggcgacct gatcatccgc
    12061 ctggacgaga cgccggtgaa gggcatggcg ctgaacgatg cggtgaagcg catgcgcggc
    12121 aagcccaata cgcagatcac cctcaccatc tcgcgcaagg gcgaggcggc gccgatcgtg
    12181 gtgaccctga cgcgcgcggt gatccgcgtg cagagcgtca agtccaagat cctcgagccc
    12241 ggctacggct gggtgcgcgt gtcgcagttc caggaatcca ccaccgagaa cctggtccgg
    12301 cacctcgaca ccctgtacaa gcagggccag ctgaagggcc tggtgctcga cctgcgcaac
    12361 gaccccggcg gcctgctgta cggcgcggtg gcgatctcct ccgccttcct gcccgccaag
    12421 gcgctggtgg tgtccaccga cgggcgcacc gaggacgcga agaagaagtt ccacgcctcg
    12481 ccggaggact actcgcgcgg cgtgcgcgac gacgtgctca ggccgctgcc ggcggcggtc
    12541 aagaacgtga agctggtggt gctggtgaac ggcggctcgg cctcggcctc ggagatcgtg
    12601 gccggcgcgc tgcaggacca caagcgcgcc accgtgatcg gcacgcagac cttcggcaag
    12661 ggctcggtgc agaccatcat gccgctgggc aattccaccg cgatcaagct gaccacggcg
    12721 cgctattaca cgccgagcgg ccgctcgatc caggcgaagg gcatcacgcc cgacatcgtc
    12781 gtcgaggagc cgggcgcgag cccgcagagc cgggtgcgcg agtcggacct cgagcgccac
    12841 ctgtccaacg accgcgacaa ggaaggcgcg aagaaacccg gcgccggcgc caagccgtcg
    12901 gccgtgccgc cgggcgagct gcccaagccg atcgagatgg gatcgaagga ggatttccag
    12961 ctcgcgcagg cgctgaactt cctcaaaggc gctccggtca agggccagcc gacgaaggcg
    13021 gtcgccgaga ccaagtccgg cagctgaacg accagcagct cctccgctac tccaggcaca
    13081 tcctcctcga ggagatcggc atcgaggggc aggagcggct gcgtgccgcc agcgcgctcg
    13141 tcgtcggcgc cgggggcctg ggctgcccgg cggcgctgta cctcgcctcc ggcggcatcg
    13201 gcaggctgac catcgccgac gccgacagcg tcgacctcac caacctgcag cgccagatcc
    13261 tctaccgcac ggattcggtc ggcgcgaaga aggtggacga ggcgaagaag gcgctcggcg
    13321 ccgtcaatcc cgaagtcgag gtggtggcgc tcgcccggcg cgtcgacggc agcctcgtcg
    13381 aggagctcgt cgccgcggcc gacgtggtgc tcgactgctc ggataatttc gccacgcggc
    13441 acgcgctgaa ccgcgcctgc gtgcagcacc gcaagccctt ggtgtcgggc gcggcgatcc
    13501 gcttcgacgg gcagctgatg gtgttcgacc tgcgcagcaa ggatgcgccc tgctacgcct
    13561 gcctgttccc cgaggacggc gaggtcgagg aagtgcagtg ctcggtgatg ggcgtcgtcg
    13621 cgccgctcac cggcgccatc ggcgcgatgc aggcgctgga ggcgatcaag ctgctcgccg
    13681 gcgcgggcga gacgccgctc ggccgcctga cgctcatcga cgccagggcc tccgagacgc
    13741 gcagcgtgcg cgtgaagaag gatccggcct gcgcggtctg cgcaggctag aggtgcgcgg
    13801 ttgagctttt ccccggcagg atggcggcgc ggtttcctgc gcgcgcagcc gctcgcggtg
    13861 ggggtgttcg gctatgcggt ggccttcggg ctgctggcct cggaggcggg cttgtcggtg
    13921 ctcgaagccg cgctgatgag cgccacggtg tattccgggt ccgcccaggt ggcgacggtg
    13981 ggcgggcttg ccgccggcgc gggcatcctc gccagcgtgg tgacggtgct gatgctcaac
    14041 gcgcgctacc tgctctacgg ggcgtcgctg cgaccctggc tgtcgcagtc cacccccggc
    14101 caggcctatg cgagcctgta cttcctcggc gacggcaact ggctgctgtc gatgaacgcg
    14161 cgcaacgccg gcgagcagga cgccggtttc gtgttcggct cgggcatcgc catgttcctg
    14221 ccctgggtcg tcggcacgct gctcggctcg gtggcgggca gctggctcgg ccggccggaa
    14281 ctgctggcgc tcgatttcct gctggtggcc ttttgcgccg ccatggcgac cgacctgttc
    14341 aagtcgcgcg gcgacctatg gaccgtcgcg gtggcgctgg cggtggccct gggcgtcgag
    14401 cgcctggcgc cgagcggctg gaccatcgtc gccaccggcc tcgccggcat cgtggcgggc
    14461 tacttccgcc acgaggagcg ggcatgacgc tgcgcctgga aattctgccg ctgatcttcg
    14521 cgctggcgct cgcctccttc gcctgccgcg ccggcggctt ctggatcatg cgcttcgtgc
    14581 gcatgacgcc gcgcatccag tcggccctgc gcgccgcacc cctggcggtg atggtgggaa
    14641 tcgtggcgcc ggcggcggcg cgcggccatc cgcccgagct gctggggatc gcggtgaccg
    14701 cggcggtgat gcgcctcacc gggagcgacc tcggcgccgc gctcgccggc gtggccacgg
    14761 tggcgttagg ccgcgcgctg ctgcgcggat agaatagccg ccgccatgaa cgccccagcc
    14821 gccgccgacc gccagcactg cgatgtgctg gtcgtgggcg gcggcccggc cggctcgacc
    14881 accgcggcgg tgctcgccga gaagggctgg cgcgtcaccc tgctggagaa ggagcgccac
    14941 ccgcgcttcc acatcggcga atccctgctg ccgcacaacc tgccgatttt caagcgcctc
    15001 ggcgtgctcg agcaggtgga cgccatcggc atcaagaagc ccggcgtgga tttcaccgcc
    15061 gagctggaag tcagcggcta ccacgagttc aagttcgcct tcgcgctgga cacgagccag
    15121 ccctatgcct acgaagtgcg tcgctccgag ttcgacaacc tgctgctgcg caacagcgcc
    15181 gccaagggcg cgcaggtgat cgagggcctg cgggtgacgc gcgtcgactt cccggagggc
    15241 ggcgacccgg tggtgcaggc ggtggacgcg gagggccggg aatgcagctg gtcctgccgc
    15301 tacttcgtcg atgccaccgg ccgcgacacg ctgctgtcga agaaattcga cttgaagcgc
    15361 aagaacaagc accacgccag cgctgcgatc ttcggccatt tcctcgacgt cgaccgccgc
    15421 gcgggcgatg acgcgggcaa catcagcctg tactggttcc agcacggctg gttctggatg
    15481 atcccgctca aggacggcag catgagcgtg ggcgcggtgt gccgccccga gtacctcaag
    15541 cagcgcagga acagcccgcc ggagttcctc atgcagacca tcgcgctcgc gccggcggaa
    15601 atgcgccacc gcatgcgcaa ggccacgctg atggacaaca tggcgcgcgc caccgggaac
    15661 tactcctacc agtcgacgcg catgcgcggc gagcgctacg tcatggtggg cgacgccttc
    15721 gccttcatcg atccggtgtt ctccagcggc gtcctgctcg gcatgaacag cggcatgctg
    15781 ggggcggaag ccgtcgatgt gtggctgcgc gagggcgccg ccgccgcgga atcgcgcttt
    15841 cgccatttcg agaggatggt gctgcgcggc gtggagacgt tttcctggtt catctggcgc
    15901 ttcaattcgc cgggcatgcg cttcctcatg ctgcagccgg gcaacccgtt ccgcgtgcag
    15961 gaggcggtaa tttcgctgct cgccggcgac gtgttccgcg acatcggcgc gcgctcgcgc
    16021 ttcgtgctgt tcaagctgtt ctacttcctg ttcagcctgc gccacccgcg cgagtcgttc
    16081 cggctctggc tgaagcgcat ccgcaacccg agcgtggcct tcaccggcgg caccacgccg
    16141 ctcgacgagc gctagccgtt ccgcgcgccg tcagtcggcg cgggtgacca tgcccggcag
    16201 gcgcgagagc ttcggcgcca ccaccggcac cgcgagcatg gtgctgccca gcgccatcag
    16261 cagcagcgcg gtgaaggtct cgctggtgat cacgcccttg tccatgagga cgttggcgaa
    16321 gatgatcatg atcaggccct tggtctgcag cagccagccg agcaccgggg cctcgcccgc
    16381 cggccagcgg aggatcttcc ccgcggcgcg cacgccgagg agcttccccc ccaccgaggc
    16441 gaagagcagc gcggcggcgg cgaggaacac cgcctcgccg cccacgctcc agttggtgcg
    16501 caggccggtg ctgagaaaga acaccggcat cgcgatgagc agcacgtggt gccgcagcag
    16561 gtccagctgc tcgcggtcga accagtcgcc gtccatcacc gcgcccgcga ggaacgcccc
    16621 gaccatgaag tgcagcccgc accagtccgc gccgaaggcg cagacggtga gccagaccag
    16681 gcccaggtac cagcggtcgc ccggcggcag ccgcagcatc aggcggcgaa agccgtaggc
    16741 ggaaatgccg aaagccgcca ggaacaccag ctgcagcccg acgcgctgcc agtccatcag
    16801 gatcagcgcc agcacgcccc agatggcgac gtcgtccagg ctcgcgtaac gcaggatgcg
    16861 ctgcccgagc ggctggcgca ggatctccag ccggtccatc agcagcagca ggaccggcag
    16921 cgcggtgacc gcgcaggcca tgcccacgcc gaggacgaac tgccagggca gcgccgccgg
    16981 gccgatccag ccgccgtagg acagcatcac cgcggcgacg ccgccgcccg ccagcagcgg
    17041 agtggcgagc gcgagcccgg cggtgatcgc gctttcgcgg cggcgcaccc aggccttcct
    17101 caggtccagc tcgatgccgg cgatccatac gaagaccatc accgcccacc aggcgatgcc
    17161 gttcagcgac tggaccaccg gctcggtgaa gacgaaggcg tagtagtccg ggaaaacctt
    17221 cccgaggatg ccgggtccga gcaggattcc ggcggcgacc tgcaccacca ccagcggcgc
    17281 gtagtagtcg gtccggccca ggcgccagat caggtacggg acggtaaaaa tgatcccgag
    17341 ggcgatgagg aacacctcca tggtgctcat ggcgcgcgat gtgccccctg cctgcggatc
    17401 acgacccagt tcagccagcc gatcgcggcg gcgacgatga tggcgtagac ctccaggccg
    17461 gagacggcgc cgaggtccag ggcgccggca agcgtcgcca gcgggaccgc ggcgcacagc
    17521 gcaattgaca ccgggatcac ccgcgcgccg aacatctgcg gtctcgtcag gagcgcgagc
    17581 agagcgccga acagcgcgag cagcacgacg ccccggccga tggacagcag cgagggatgc
    17641 atcgcgaaca gcggccaggt cgtcagcaat cccagcacgt ccgccgcgat ggcaagaccg
    17701 agcagcgccc ggtccgcgag acgcctcggc ttcgccagtt ccccgggctt gcccgccagc
    17761 tgctccgcca tcaccgcgag gaccacggcg aaccagacca tgaacccgat cgccaggtag
    17821 ccgagcgcgc cgatgcccgt caggtggccg aaaccgatgg cggccgacat gacgagcagg
    17881 aaccagacga tcgattcgac ggcgaccctg gccttggcgc gttcctcgtg ccgccagccg
    17941 tcgacggtgg ccttgagccg gcgatacatc gcgcgcgcat gcatgtgcgc cgacttgcgc
    18001 gcgacgtact gctgcagctc ggcgccgtcg tccccggtct cgggcgggct aggcaaggaa
    18061 cttgtcgccg cgcatgggcc gcagctacgc ggagaggtag ccccgctgcg cggcgtagcc
    18121 ctcgctcggc ttcttggcga agccgctctt cgcgaactga tgcagcgagc cgatggcgta
    18181 gcgcagcagc accgaggcgc ggcccgcggc gtcgccgtcc gacgacacgc gcagcgcctg
    18241 cttgagcgtg gcctcgaagc ggtcgtagaa ctggttcatg cgcgcctgca gccgctcgtg
    18301 ctcgccgacc agggcgtcgc cggtcatcac ccgcaccatg ccgggattct tctcggcgaa
    18361 ggcgagcagc atctccagca gctggtcggc ctgtttgcgc ccgtcgggct cgtccgccgt
    18421 gatcctgttg gcgacggaaa acaccgagct ctcgatgaac tcgatcaggc cctcgtacat
    18481 ctgggccttg gaggcgaaat ggcggtacag ggcggcctcg gagacgtcga gtttcgccgc
    18541 cagcgcggcg gtggtgattc tttcccagcg cggcgcttcg agcatctggg cgagcgcctt
    18601 gaggatttcc agcctgcgct cgcctttgac ccggggcatg aaaagattct actagtgtga
    18661 atagcgaaaa acgagtcccg gcaactgcgt gaccgacgcg acgcgcaaat cgacgtaggg
    18721 aacgttcttc ctctcccggc tgacccagac tgtcgccatt cccagtcgct ttgcggtgcg
    18781 caaattctcc agcatgtcgt ccaccagggc gcagcgcgcg gcttcgaggt ggtgccggcg
    18841 cagcaacagc cggaatcccg acagcgaggg tttgccccgg tagcggctgc tctcgatcga
    18901 gtagacggcg tcgaaatagc gcgccacgcc gaggatcctc accactgcgt ccacgtagtg
    18961 gcgcggcgca ttggaataca ggatcttgcg cccggggagg cgggcgagcg catggcgcac
    19021 cgcgttttcg tgcaccacca tgtcggcgag ttcggggaac tggtgcgtgg cccagaggaa
    19081 ctcggcccgg ctcgcgccgt ggtggcgcgc gaggccctcc atggtggtgc cgtatttctc
    19141 ccagaagccg cgccgcatcg cgttcgcgcc cgcctcgtcg acggcgaagc ggcgcatcag
    19201 gtaggcgttg atctgatcgt gcatcgccgg aaagatgcgc gcctgcgcat cgtgcagcgt
    19261 gttgtcgaga tcgaaaaacc acacccgccg cgccggcggc agcccgacga ggcgcatgcg
    19321 gctacttgct cttgatcagg gtgccgacgc cctggtccgt catcacctcg agcagcagcg
    19381 cgttgggcac gcggccgtcg atgatgtgca cgctcttcac gccgttgcgc gcggcgtcga
    19441 gcgcggagcc gatcttgggc agcatgccgc cggagatcac gcccttcgcg accaggtcgt
    19501 cgattctgcg cggcgtgagc ccggtaagca gcttgccgtc cttgtccagc acgcccggcg
    19561 tattggtcag aaccacgagc ttctcggcgc gcagcacctc ggccagcttc ccggcgacaa
    19621 ggtcggcgtt gatgttgtag gtcgtaccgt ccgcgccggt tgcgatcggc gccaccaccg
    19681 ggatgaaccc gccctgctcg agggcgccga tgaccgacgg gtcgatcgac tcgatgtcgc
    19741 cgacctgccc gatgtccacc ttgccgttct cgcgcgaggg cagcagcatc ttgcgcgccc
    19801 ggataaaagc cccgtcctgg ccggtgaggc cgaccgcctt gccgccggcc tggttgataa
    19861 gctcgaccac ctccttgttg acctggccgc cgagcaccat ttcgaccaca tccatggtct
    19921 cgcggtcggt
//
LOCUS       DASRIE010000235        13645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1297288, whole genome shotgun sequence.
ACCESSION   DASRIE010000235 DASRIE010000000
VERSION     DASRIE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297288"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..744)
                     /locus_tag="VFB53_10030"
     CDS             complement(199..744)
                     /locus_tag="VFB53_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HZN87108.1"
                     /translation="MKAQERNELLRVLEARFEKNMHRHKGIGWAEVRARLESHPSALR
                     SLRAMEATGGEPDVIGRDKETGRFTFCDCSTESPTGRRSLCYDRAALDSRKEHKPQSS
                     AVEMAGEMGIDLLTEEQYRELHKLGEFDTKTSSWVRTPPEVRSLGGALFCDRRYGRVF
                     VYHNGAQSYYAARGFRGLLSV"
     gene            complement(744..2141)
                     /locus_tag="VFB53_10035"
     CDS             complement(744..2141)
                     /locus_tag="VFB53_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN87109.1"
                     /translation="MKDLHYAGAKQLARLIRTRKVSAAEVMRAFIERIERLNPKVNAI
                     VTFLPEQALQEAKALDRSRKYGPLAGLPIAYKDLVATRGIRTTLGSMVYKDHLPREDA
                     LLVERLKAAGAITLGKTNTPEFGAGSNTFNKVFGATRNPYDLSKTSGGSSGGAGAAVA
                     CGMLPFADGSDLAASLRNPGNYCNVVGFRPTPGRVPTWPAANAWDTQPVIGPVARTVE
                     DAAFLLSAMAGPDRRAPVSIAEPGSIFSKPLKRSFRKVRIAWTKDFGGLPMEPQVTAV
                     LEKQKKVFESLGCIVEEDCPDFTGATEAFETLRAVSFALRFAPLLKTHRALLKDTVVW
                     NIEQGLALDGAKIGRAELLRTELYQRLRAFLEKYEFLLGPVNQLAPFPVETEYPTEIA
                     GVKLHNYLDWMKSCYYITITSHPAISVPAGFTPGGLPVGLQIVGRYRDDFGLLQLAHA
                     FEGETGVWKRRPALA"
     gene            complement(2149..3606)
                     /gene="rng"
                     /locus_tag="VFB53_10040"
     CDS             complement(2149..3606)
                     /gene="rng"
                     /locus_tag="VFB53_10040"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZN87110.1"
                     /translation="MSEEILVNITPQETRVAVVQAGAVQELLVERAASRGLVGNIYMG
                     RVARVLPGMQSAFVEAGLERAAFLHVADIWEQRKANGEAPRPIEKVLAEGQPVLVQVV
                     KDPIGSKGARLTTQVSIAGRMLVYLPQDPHIGISQRIEDEGGRQALRDRLKALLPADE
                     KGGFIVRTLAEGADEAELRADVEYLRHLWHDIRERSLGAEPPRLLYQDLSLAQRVLRD
                     MVHGDTAVVKVDSRENYQQLAAFAGSYMPEVRARLEHYTGERPLFDLYNVEAEIEKAL
                     SRRVDLKSGGTLFVDQTEAMTTIDVNTGGFVGQRNFDDTIFKTNLEAAQSIARQLRLR
                     NLGGIVIVDFIDMQSEEHRGAVLEELRKALARDRTRMTVSGFTQLGLVELTRKRTRES
                     LAHVLCEPCPTCAGRGEVKTARTVCYEILREILREARAFNAREFRVLGAQGVVDLLQE
                     EESGSVAMLSDFIGKPISLQVEPGYTQEQFDIVLM"
     gene            complement(3584..4222)
                     /locus_tag="VFB53_10045"
     CDS             complement(3584..4222)
                     /locus_tag="VFB53_10045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZN87111.1"
                     /translation="MAQPLDRSIYLASRSPRRRELLGHIGVKFHLLLFRNRAGAAPDV
                     DEEPLPEETPRDYVMRVARAKAAVGWQRMQERNLPRHPVLAADTTVALEGRIYGKPAD
                     RAEAAAMLGALSGKRHEVLTAVALQYDDEVETALSVSEVQFRELSAQDIQDYVATGEP
                     DDKAGAYAIQGRAALFVSEIRGSQSGIVGLPLYETAQLLQRMGTRRERRNTR"
     gene            complement(4253..4651)
                     /locus_tag="VFB53_10050"
     CDS             complement(4253..4651)
                     /locus_tag="VFB53_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZN87112.1"
                     /translation="MAKLLLVAHGARLRGWAREARDQYLDRMPRGYAVECLPRAPQGA
                     RIVALDERGRDLTTAQLAAQIGKWIGESAPIAFVIGGPDGLDAQTRKEAALLLRLSAL
                     TLPHALAQVLLAEQLYRAATLLTGHPYHRD"
     gene            complement(4617..5006)
                     /gene="rsfS"
                     /locus_tag="VFB53_10055"
     CDS             complement(4617..5006)
                     /gene="rsfS"
                     /locus_tag="VFB53_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZN87113.1"
                     /translation="MDIRRKQRAVVEALEDVKGHDIVVFNVARLSPFFERVVIASGDS
                     SRQVKALADSVQDRLSSLGARVYGIEGQATGDWVLVDLGDIVVHVMHPAVRSHYNLEE
                     IWGGKPVKLAGPAKVARGKAAARRARR"
     gene            complement(4993..5541)
                     /gene="nadD"
                     /locus_tag="VFB53_10060"
     CDS             complement(4993..5541)
                     /gene="nadD"
                     /locus_tag="VFB53_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HZN87114.1"
                     /translation="MSIGILGGTFDPVHNAHLAMARAALEQLGLERILWMPTGAPKYR
                     KPARASAAQRLAMLRLAVAGEPRYAIDERELAPGHSGYTVDSLQGFAEKPVLLLGADQ
                     YAEFEHWHRWREVLERASLAVFARPGSPAPDGRVRHAAFEPMDMSASDIRARAARGES
                     IAGLVPPAVAGYIAQYHLYGHP"
     gene            complement(5538..6716)
                     /locus_tag="VFB53_10065"
     CDS             complement(5538..6716)
                     /locus_tag="VFB53_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN87115.1"
                     /translation="MSAAPGAPRPRRILPVIIASQFAGASVWFAGNAVLADLQRQWGL
                     PAEALGYVTSAVQLGFIAGTLAFAFFAVADRYSPRLVFLLCAFAAALANAAALALPES
                     LGGLLALRFATGFFLAGIYPVGMKIASGWYQRDLGNALGFLVGALVAGTAFPHLVRGL
                     GGDLPWQGVLAAASLVAAAGGVLMYALVPDGPHLARGARFDPRALAAVFRVPAFRASA
                     FGYFGHMWELYAFWAFVPWVLAARGISDVPLWSFAVIGAGALGCAAGGLASLRRGSAP
                     VAFAQLAASGACCLASPLAFFAPAPVFLAFLLFWGVVVVGDSPQFSALNAQSAPRERV
                     GSALTIANCIGFGITIFSIELLNAASGVLAPQWLFLLLVPGPVLGLAALRPLLGRAAA
                     "
     gene            complement(6713..7981)
                     /locus_tag="VFB53_10070"
     CDS             complement(6713..7981)
                     /locus_tag="VFB53_10070"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZN87116.1"
                     /translation="MDQKLDLKSYVRGLGQAARVAARLLARAEPDAKNRALAAMAAEL
                     RAQREPLLEANRGDVAQAKKDGRDAAFVDRLTLTPALIEQMAVGLEQVALLPDPVGQV
                     SERVRRPSGIEVARMRVPLGVIGIIYESRPNVTADAAGLCVKAGNACILRGGSEALHS
                     NRAIAACVRAGLKAAGLPEAAVQMVEVADRAAVGELIQLHEFVDIIVPRGGKELISRL
                     MRESRIPMLKHLDGVCHVYVDDRADPAMAVRIADNAKTQRYGTCNTMETLLVAEGIAP
                     RVLPEIGRIFRDKGVEMRADAAARKLLPQAKAATEQDYATEWLAPVVSVRVVPGLDEA
                     IAHIAQYGSQHTDAIVTEDAARAQRFLREVDSSSVLVNASTRFADGYEFGLGAEIGIS
                     TDKLHARGPVGLEGLTTQKFVVLGHGEIRS"
     gene            complement(7965..8969)
                     /gene="holA"
                     /locus_tag="VFB53_10075"
     CDS             complement(7965..8969)
                     /gene="holA"
                     /locus_tag="VFB53_10075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZN87117.1"
                     /translation="MQLRAEQLDAHLAKSFAALYVIHGDEPLIALEAADAVRAAARKR
                     GHDEREVYTAERGFDWSEFAHAGASRSLFGGAKIVELRLPSGKPGTEGAAAIAAYCAR
                     LDPQMLTLVSLPRLDRAAQASPWFGALAGAGVVVEVWPVERARLPEWIGARLARQGQK
                     ASREALAFLADRVEGNLLAAHQEVQKLALLAPPGELSLETLEGAVATVARYDPYAAAE
                     ALVAGDLARYVRILQGLRGEGEAPTFVLFALASALFVLRGATEGKPVERLFGEHRLFN
                     KPLQRAVQAAARRHARAALESALAQAALIDRVIKGVARGDPWDQFTALGVRLALGPKA
                     "
     gene            complement(8953..9456)
                     /gene="lptE"
                     /locus_tag="VFB53_10080"
     CDS             complement(8953..9456)
                     /gene="lptE"
                     /locus_tag="VFB53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZN87118.1"
                     /translation="MRPALAAALALALAACGFQLRGTASLPFETLYLPGATSGIALDL
                     KRSIQSGTATRVVDDAANAQAQLLFSEETRAKEILSLTAAGRVREFRLVYRVGFRITD
                     GKGGDFLASSTVALTRDVTYDDTVVLAKEAEEQLLFREMQADMVQQILRRIAAAKKPA
                     APDATAR"
     gene            complement(9453..12011)
                     /gene="leuS"
                     /locus_tag="VFB53_10085"
     CDS             complement(9453..12011)
                     /gene="leuS"
                     /locus_tag="VFB53_10085"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZN87119.1"
                     /translation="MQPQYEPQQVEREAQAFWTQRQSFRSREEPGKPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRHHRMRGLNVLQPMGWDAFGLPAENAAMANRVPPAKWTY
                     DNIATMKRQMQGLGFALDWEREFATCRPEYYKWNQWLFTRLFRKGLAYKKTGVVNWDP
                     ADQTVLANEQVIDGKGWRTNAPVEKREIPMYFFRITAYAEELLGALEKMDGWPEQVKL
                     MQKNWLGRSEGVRIGFPYELDGAKKTLWVFTTRADTLMGATFMAVAAEHPIAAHAAKR
                     DAKLAAFVEQCRKGAVMEADLAQLEKKGMPTGLYCSHPLTGEKLPVWVGNYVLMGYGE
                     GAVMGVPGHDERDYAFALTYALPIKCVIRTPAGDSVPEPWRPEYAEYGTCINSGKYDG
                     LDYQAAVDAIAADLKAKGLGEKQVQWRLRDWGISRQRYWGCPIPIVHCASCGDVPVPD
                     EQLPVKLPEHLVPDGTGNPLAKMPEFYETRCPACGKPAKRETDTMDTFVDSSWYFARF
                     ACPDNERAMLDARAKYWMPVDQYIGGIEHAILHLLYSRFFTRAMKAEGLLGVEEPFAN
                     LLTQGMVLAESYYSEAGGRREWINPAEVEVTRDAKGKIVAAQAKDGRAVVYDGIGTMS
                     KSKNNGVDPQALIDRYGADTARFYVIFASPPTNTLEWSDESVEGSFRFLKRVWAYAAK
                     FENPGPAMADELAKAIRFEIHSVLRQANYDLSKHQFNTVASAAMKILNALERFAGGKN
                     KVTEEGLSILLRLLAPITPHLAHYLWRELGFGEDVMRASWPEPEAAALEQDEIDYVVQ
                     VGGKTRGNMRAPRASDQPALEKMVRESELVQKYASGKPIKRIVVVPGRLINVVV"
     gene            12105..12986
                     /locus_tag="VFB53_10090"
     CDS             12105..12986
                     /locus_tag="VFB53_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZN87120.1"
                     /translation="MKRILGLLAAAALALVTTQGLAAAAAVVEGVQMPAWVERASAGA
                     TRRLPLAPGMELRGGDEVRTGSGSRLYIKLAEGSLVKLGENASLKILDIAPDRGGFFK
                     AALNVLEGAFRFTTDVLAKERRRDVSIRVATVTAGIRGTDLWGKSTAERQIVCLIEGK
                     IEVGAEGEAPVTMDQARQFYQRDKGQTQPVAFVDPVQLGQWARETEIDEGKGAARSGG
                     KWRVTLASVESQSEALAVYDTLRTAGFAAEIFPSKSGEKRVYLVRISRLASKADAEAL
                     AVQLRGTAGVQEPKVSG"
     gene            13116..>13645
                     /locus_tag="VFB53_10095"
     CDS             13116..>13645
                     /locus_tag="VFB53_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN87121.1"
                     /translation="MRIVVAFAAGGSTDVAVRSIQESLARSFGQPVIVENRTGAAGNI
                     AAEYVVKQPADGYLVLASADSLASNPHLFKNLPFDPAKDLVPVVQLTRQPVVLAVHPS
                     TGVSNVAELVAAAKAKPGMGYATSGAGSIQHMAGEWFAQLAGIKLTHVPYKGGGQAIT
                     DLVGGQVPIGSLSNTPL"
BASE COUNT         1936 a         4759 c         4824 g         2126 t
ORIGIN      
        1 ctcttcatga tctcgtctcc tctcgctctc gtcgggcctc gggaatctcg gcctccaagc
       61 ccattagact ttccgggcaa gcctggtgtg accagacaac cccctgagga gatgcggggg
      121 ccgtttaacg ccttggtgaa cggcgggccg aattggccta ttcgttccac cgcgtgagct
      181 tgcgaaccgg ctgcgcgctc acacagagag caagccgcgg aagcctctcg ccgcatagta
      241 cgactgcgcg ccgttgtggt acacgaacac tctgccatag cggcgatcac agaagagcgc
      301 gccgccgagc gatcgaacct cgggcggcgt tctgacccag ctggaggttt tcgtgtcgaa
      361 ctcgccaagc ttgtgcagtt cccggtactg ctcttcagtc aacaggtcga tgcccatttc
      421 gccggccatt tcgaccgcac tgctctgcgg cttgtgctcc ttccgtgagt ccagggccgc
      481 gcggtcgtag cagaggcttc tccggcctgt cgggctctcc gtggagcaat cgcagaacgt
      541 aaagcgccca gtctccttgt cgcggccgat cacgtcgggc tccccgccgg tcgcctccat
      601 cgcacggagc gacctcagtg cgctggggtg gctttcgagc ctggctcgga cttcggccca
      661 tccgatgcct ttgtggcggt gcatgttttt ctcgaaccga gcctccagta ctcgaagcag
      721 ctcgttgcgc tcttgtgctt tcatcatgcc agcgccggcc gccgcttcca gaccccggtc
      781 tcgccctcga aggcgtgcgc gagctgcagc aagccgaagt cgtcgcggta gcgccccacg
      841 atctgcaggc cgacaggcag gcccccgggc gtgaagcccg ccggcaccga gatcgccggg
      901 tggctggtga tggtgatgta gtagcagctc ttcatccagt cgagatagtt gtgcagcttc
      961 acgccggcaa tctcggtcgg gtattcggtc tccaccggga agggggcgag ctggttcacc
     1021 ggcccaagga ggaactcgta cttctccagg aaggcgcgca ggcgctggta gagctcggtg
     1081 cgcaggagct cggcgcggcc gatcttcgcg ccgtcgaggg cgaggccctg ctcgatgttc
     1141 cagaccacgg tatccttgag cagggcgcgg tgggtcttca acaggggcgc gaagcgcagg
     1201 gcgaaggaca ccgcgcgcag cgtctcgaag gcttcggtgg cgccggtgaa atccgggcag
     1261 tcttcctcca cgatgcagcc gagcgattcg aagacctttt tctgtttctc cagcaccgcg
     1321 gtgacctgcg gctccatcgg caggccgccg aagtccttcg tccaggcgat gcgcaccttc
     1381 ctgaagctgc gcttgagtgg ttttgaaaag atgctgccgg gttcggcgat cgacaccggc
     1441 gcgcgacggt cgggccccgc catcgccgag agcaggaacg ccgcgtcctc caccgtgcgc
     1501 gcgaccgggc cgatcaccgg ctgcgtgtcc caggcgttcg ccgccggcca ggtcggcacg
     1561 cggcccggcg tcggccggaa gcccaccacg ttgcagtagt tgcccggatt cctcaggctc
     1621 gcggcgaggt ccgagccgtc ggcgaagggc agcatgccgc aggccaccgc cgcgccggcg
     1681 ccgcccgagg agccgccgga ggttttcgac aggtcgtagg gattgcgcgt cgcgccgaac
     1741 accttgttga aggtgttgct gccggcgccg aactctgggg tgttggtctt gccgagcgta
     1801 atggcgcccg cggccttcag gcgctcgacg agcagcgcgt cctccctggg caggtggtcc
     1861 ttgtagacca tcgagccgag cgtggtgcgg atgcccctgg tcgccaccag gtccttgtag
     1921 gcgatcggca ggccggcgag cgggccgtat ttcctgctgc gatccagcgc cttcgcctcc
     1981 tgcagcgcct gctcgggcag gaaggtgacg atggcattga ccttggggtt caggcgctcg
     2041 attctctcga tgaaggcgcg catcacctcg gcggcggaaa ccttgcgcgt gcggatcagg
     2101 cgggcgagct gcttcgcccc ggcgtagtgc aagtccttca tgggcctcct acatcaacac
     2161 gatgtcgaac tgctcctgcg tgtatcccgg ctcgacctgc agcgagatcg gcttgccgat
     2221 gaagtccgag agcatggcca ccgagcccga ctcctcctcc tgcagcaggt cgaccacgcc
     2281 ctgcgcgccg agcacgcgga actcgcgcgc gttgaaggcg cgcgcctcgc gcaggatctc
     2341 gcgcaggatc tcgtagcaca cggtgcgcgc ggtcttcacc tcgccgcggc cggcgcaggt
     2401 ggggcagggc tcgcacagca cgtgggcgag cgattcgcgc gtgcgcttcc gggtcaattc
     2461 caccaggccg agctgcgtga agccgctcac cgtcatgcgc gtgcggtcgc gcgccagcgc
     2521 cttcctcagc tcctccagca ccgcgccgcg gtgctcctcg gactgcatgt cgatgaagtc
     2581 gacgatcacg atgccgccca ggttgcgcag gcgcagctgg cgcgcgatgg actgcgccgc
     2641 ctccaggttg gtcttgaaga tggtgtcgtc gaaattgcgc tggccgacga agcccccggt
     2701 gttgacgtcg atggtggtca tcgcctcggt ctggtcgacg aacagcgtgc cgccggactt
     2761 caggtccacg cgccgcgaca gcgccttttc gatctcggcc tcgacgttgt acaggtcgaa
     2821 cagcggccgc tcgccggtgt agtgctcaag gcgcgcgcgc acctcgggca tgtagctgcc
     2881 ggcgaaggct gcgagctgct ggtagttctc gcgcgagtcc accttcacca ccgcggtgtc
     2941 gccgtgcacc atgtcgcgca gcacgcgctg cgccagcgac aggtcctggt acagcagccg
     3001 cggcggctcg gcgccgagcg agcgttcgcg gatgtcgtgc cacaggtggc gcaggtactc
     3061 cacgtcggcg cgcagctcgg cctcgtcggc gccctcggcg agcgtgcgca cgatgaaccc
     3121 gcccttctcg tcggcgggca gcagggcctt gagccggtcg cgcagcgcct ggcggccgcc
     3181 ctcgtcctcg atgcgctgcg agatgccgat gtgcgggtcc tgcggcaggt agaccagcat
     3241 ccgcccggcg atcgacacct gggtggtgag gcgcgcgccc ttggagccga tcgggtcctt
     3301 caccacctgc accagcaccg gctggccctc ggcgagcacc ttctcgatcg gccgcggcgc
     3361 ctcgccgttc gccttgcgct gctcccagat gtcggcgacg tgcaggaagg cggcgcgctc
     3421 caggcccgcc tcgacgaacg ccgattgcat gcccggcagc acgcgcgcca cgcgccccat
     3481 gtagatgttg ccgaccaggc cgcggctcgc cgcgcgctcc accagcagct cctgcaccgc
     3541 gccggcctgc accaccgcca cgcgcgtctc ctgcggggtg atgttaacga gtatttcttc
     3601 gctcacgccg cgttcccatc ctctgcagca gctgggcggt ctcgtacagc ggcagcccga
     3661 cgatgcccga ctggctgccg cggatctcgc tgacgaaaag cgccgcgcgc ccctggatgg
     3721 cgtaggcccc ggccttgtcg tcgggctcgc cggtggcgac gtagtcctgg atgtcctggg
     3781 cgctgagctc gcggaactgc acctccgaca ccgacagcgc cgtctccacc tcgtcgtcgt
     3841 actgcaaggc caccgcggtg agcacctcgt ggcgcttgcc ggagagcgcg ccgagcatgg
     3901 cggcggcctc ggcgcggtcg gccggcttgc cgtagatgcg cccttcgagc gcgaccgtgg
     3961 tgtcggcggc gagcaccggg tgccgcggca ggttgcgttc ctgcatgcgc tgccagccga
     4021 ccgccgcctt ggcgcgcgcc acgcgcatca cgtagtcgcg cggcgtctcc tccggcaggg
     4081 gctcctcgtc gacatccggc gccgcgccgg cgcggttgcg gaacagcagc aggtggaact
     4141 tgacgccgat gtggccgagc aattcgcggc ggcgcgggct gcgcgaggcg aggtagatgc
     4201 tgcggtcgag cggttgtgcc atggctgcgc cgagccggcg agtgtagcaa agctaatctc
     4261 tatggtaggg gtgcccggtg agcagcgtcg ccgcgcggta cagctgctcg gcgagcagca
     4321 cctgggcgag ggcgtgcggc agcgtgaggg cggacaggcg cagcagcagc gccgcctcct
     4381 tcctggtttg cgcgtcgagc ccgtcggggc cgccgatgac gaaggcgatg ggcgccgatt
     4441 cgccgatcca cttgccgatc tgcgccgcga gctgcgcggt ggtgaggtcg cgcccgcgct
     4501 cgtccagcgc gacgatgcgc gcgccctgcg gcgcgcgcgg caggcactcg accgcgtagc
     4561 cgcgcggcat gcggtcgagg tactggtcgc gcgcctcgcg cgcccagccg cgcagcctag
     4621 cgccgtgcgc gacgagcagc agctttgcca cgcgccacct tcgccgggcc ggccagcttc
     4681 acgggcttgc cgccccagat ctcctccagg ttgtaatggc tgcgcaccgc cgggtgcatc
     4741 acgtgcacca cgatgtcgcc caggtccacc agcacccagt cgcccgtcgc ctggccttcg
     4801 atgccgtaga cgcgcgcgcc cagggaactc agccggtcct gcacgctgtc ggcgagcgcc
     4861 ttcacctgcc ggctcgagtc gccgctggcg atcaccacgc gctcgaagaa cggcgacagg
     4921 cgggcgacgt tgaacaccac gatgtcgtgg cccttcacgt cctccagggc ctccaccacc
     4981 gcgcgctgct tcctacggat gtccatacag gtggtattgg gcgatgtagc cggcgaccgc
     5041 cggcggcacc agcccggcga tgctctcgcc gcgggcggcg cgcgcgcgga tgtcgctcgc
     5101 cgacatgtcc atgggctcga acgccgcatg gcgcacgcgg ccgtccggcg ccgggctccc
     5161 ggggcgcgcg aacacggcga ggctggcgcg ctcgagcacc tcgcgccagc ggtgccagtg
     5221 ctcgaactcg gcgtactggt cggcgccgag cagcagcacc ggcttctcgg cgaagccctg
     5281 caggctgtcc accgtgtagc cggaatggcc tggcgcgagc tcgcgctcgt cgatggcgta
     5341 gcgcggctcc cccgcgacgg cgaggcgcag catggcgagg cgctgcgcgg cgctcgcgcg
     5401 cgccggcttg cggtacttcg gtgccccggt cggcatccac aggatgcgct ccagccccag
     5461 ctgctccagc gcggcccgcg ccatggcgag gtgcgcgttg tgcaccggat cgaaggtgcc
     5521 gccgaggatg ccgatgctca tgcggcggcg cgcccgagca ggggccgcag cgcggcgagg
     5581 ccgagcaccg ggcccggcac cagcaggagg aacagccatt gcggcgcgag gacgcccgag
     5641 gccgcattga gaagctcgat gctgaagatg gtgatgccga agccgatgca gttggcgatg
     5701 gtgagcgccg agcccacgcg ctcgcgcggc gcgctctgcg cgttcagggc cgagaactgc
     5761 ggggagtcgc ccaccaccac cacgccccag aatagcagga aggcgaggaa caccggcgcc
     5821 ggcgcgaaga aggcgagcgg cgaggcgagg caacaggccc ccgaggccgc gagctgcgcg
     5881 aaagccaccg gcgcgctgcc ccggcgcagc gacgccaagc cgcccgccgc gcagccgagc
     5941 gcgcccgcgc cgatgaccgc gaagctccac agcggcacgt cggagatgcc gcgcgcggcg
     6001 agcacccacg gcacgaaggc ccagaaggcg tagagctccc acatgtggcc gaaatagccg
     6061 aaggccgagg cgcggaaggc gggcacgcgg aacacggcgg cgagggcgcg cgggtcgaag
     6121 cgcgcgccgc gcgcgaggtg cgggccgtcg ggcaccagcg cgtacatcag cacgccgccc
     6181 gccgcggcga ccagcgaggc cgcggcgagc acgccctgcc agggcaggtc cccgcccagc
     6241 ccgcgcacca ggtgcggaaa ggcggtgccc gcgacgagtg cgccgaccag gaacccgagc
     6301 gcgttgccca ggtcgcgctg gtaccagccc gaggcgatct tcatgcccac cgggtagatg
     6361 ccggcgagga agaagcccgt ggcgaagcgc agcgcgagca ggccgccgag gctctcgggg
     6421 agcgcgagcg ccgcggcgtt ggcgagcgcg gcggcgaagg cgcagagcag gaacaccagg
     6481 cgcggcgagt agcggtcggc gaccgcgaag aaggcgaagg cgagggtgcc ggcgatgaac
     6541 ccgagctgca ctgccgaggt gacgtagccg agcgcctccg ccggcaggcc ccactggcgc
     6601 tgcaggtccg cgagcaccgc gttgccggcg aaccagaccg aggcccccgc gaactgcgag
     6661 gcgatgatga ccggcaggat gcgccgcggc cgcggcgcgc cgggcgccgc gctcagctgc
     6721 ggatctcgcc gtggcccagc accacgaact tctgcgtggt gaggccctcc agcccgaccg
     6781 ggccgcgcgc gtgcagcttg tcggtggaga tgccgatctc ggcgccgagg ccgaactcgt
     6841 agccgtcggc gaagcgcgtc gaggcgttca ccagcaccga gctggaatcg acctcgcgca
     6901 ggaagcgctg cgcgcgcgcc gcgtcctcgg tgacgatggc gtcggtgtgc tgcgagccgt
     6961 actgcgcgat gtgcgcgatc gcctcgtcga gccccggcac cacgcgcacc gacaccaccg
     7021 gcgcgagcca ttcggtggcg tagtcctgct cggtggcggc cttggcctgc ggcaggagct
     7081 tgcgcgccgc ggcgtcggcg cgcatctcca cgcccttgtc gcggaagatg cggccgatct
     7141 ccggcagcac ccgcggcgcg atgccttcgg ccaccagcag cgtctccatg gtgttgcagg
     7201 tgccgtagcg ctgcgtcttg gcgttgtcgg cgatcctcac cgccatcgcc gggtcggcgc
     7261 ggtcgtcgac gtacacgtgg cacacgccgt ccaggtgctt gagcatcggg atgcgcgact
     7321 cgcgcatcag gcggctgatc aattccttgc cgccgcgcgg cacgatgatg tccacgaact
     7381 cgtgcagctg aatgagttcg cccaccgcgg cccggtcggc gacctccacc atctgcaccg
     7441 ccgcctccgg cagccccgcg gctttcagtc ccgcgcgcac gcaggcggcg atcgcccggt
     7501 tggagtgcag cgcttcggag ccgccgcgca ggatgcaggc gttgcccgcc ttcacgcaca
     7561 ggccggcggc gtccgcagtg acgttggggc gcgactcgta gatgatgccg atcactccga
     7621 gcggcacgcg catgcgcgcc acttcgatgc cgctggggcg ccgcacgcgc tccgagacct
     7681 gcccgaccgg gtcgggcagc agcgccacct gctccaggcc aacggccatc tgttcgatga
     7741 gcgccggcgt gagcgtgagc cggtcgacga aggcggcgtc gcggccgtcc tttttcgcct
     7801 gcgccacgtc gccgcgattg gcctcgagca ggggctcgcg ctgcgcgcgc agctcggcgg
     7861 ccatcgccgc gagcgcgcgg ttcttggcat cgggctcggc gcgcgccagc aggcgcgcgg
     7921 cgacgcgcgc cgcctggccg aggccgcgca cgtaggactt caggtcaagc ttttggtcca
     7981 agcgccaatc ttaccccgag ggcggtgaac tgatcccagg ggtcgccgcg cgccacgccc
     8041 ttgatcacgc ggtcgatgag ggcggcctgc gcgagcgcgg actcgagcgc cgcgcgcgcg
     8101 tggcgccgcg cggccgcctg caccgcgcgc tgcagcggct tgttgaacag gcggtgctcg
     8161 ccgaacaggc gctccaccgg ctttccctcg gtggcgccgc gcagcacgaa gagggcgctc
     8221 gcgagcgcga acagcacgaa ggtcggcgcc tcgccctcgc cgcgcaggcc ctgcaggatg
     8281 cgcacgtagc gcgcgaggtc gccggcgacc agcgcttccg ccgcggcata ggggtcgtag
     8341 cgcgccaccg tcgcgaccgc gccttccagc gtctcgagcg agagttcgcc cggcggggcg
     8401 agcagcgcga gcttctgcac ttcctggtgg gcggcgagga ggttgccttc cacgcggtcg
     8461 gcgaggaagg cgagcgcttc gcgcgaggcc ttctggccct ggcgcgcgag gcgcgcgccg
     8521 atccattccg gcagccgcgc gcgctccacc ggccacacct cgaccaccac gccggcgccg
     8581 gcgagcgcgc cgaaccacgg cgaggcctgc gccgcgcggt ccagccgcgg caggctcacc
     8641 agggtcagca tctgcgggtc gagccgcgcg caataggcgg cgatggcggc ggcgccctcg
     8701 gtgccgggct tgccgcttgg caggcgcagc tcgacgatct tggcgccgcc gaagagcgag
     8761 cggctggcgc cggcgtgcgc gaattcgctc cagtcgaagc cgcgctcggc ggtgtacacc
     8821 tcgcgctcgt cgtggccgcg cttgcgcgcc gcggcgcgca ccgcgtcggc cgcctcgagc
     8881 gcgatcaggg gctcatcgcc gtggatcacg tacagcgcgg cgaaagattt cgccaggtgc
     8941 gcgtcgagct gctcagcgcg cagttgcatc gggggcggcg ggtttcttgg cggcggcgat
     9001 gcgccgcagg atctgctgca ccatgtcggc ctgcatctcg cggaacagga gctgctcctc
     9061 ggcctccttg gccagcacca ccgtgtcgtc gtaggtgacg tcgcgggtca gcgccaccgt
     9121 gctcgaggcg aggaagtcgc cgcccttgcc gtcggtgatg cgaaagccca cgcggtacac
     9181 cagacggaat tcgcgcacgc gccccgcggc ggtgagcgag aggatctcct tggcgcgcgt
     9241 ctcttcgctg aacagcagct gcgcctgcgc gttcgcggcg tcgtccacca cgcgggtggc
     9301 ggtgcccgac tggatgctgc gcttcaggtc gagcgcgatg ccgctggtgg ccccgggcag
     9361 gtacagggtc tcgaacggca ggctggctgt gccgcgcagc tggaagccgc aggcggcgag
     9421 ggcaagcgcg agtgcggctg cgagtgcggg tctcaaacga cgacgttgat caggcggccg
     9481 gggaccacga cgatgcgctt gatcggcttg ccgctggcgt acttctgcac cagttcgctt
     9541 tcgcgcacca tcttctccag cgcgggctgg tcggaggccc gcggcgcccg catgttgccg
     9601 cgggtcttgc cgcccacctg caccacgtag tcgatctcgt cctgctcgag cgccgcggct
     9661 tcgggctcgg gccaggacgc gcgcatcacg tcctcgccga agccgagctc gcgccacagg
     9721 tagtgcgcga ggtgcggcgt gatcggcgcg agcaggcgca gcaggatcga caggccttcc
     9781 tccgtgacct tgtttttccc cccggcaaat ctttcgagcg cgttcaggat cttcattgcc
     9841 gccgaggcga cggtgttgaa ttgatgcttg gacagatcgt agttcgcctg cctcagcacg
     9901 gaatggattt cgaaccggat tgccttggca agttcatccg ccatggcagg tcccgggttt
     9961 tcgaacttcg ccgcgtacgc ccagacgcgc ttcaggaacc tgaacgagcc ctcgacgctc
    10021 tcgtccgacc attccagcgt gttggtcggc ggcgaggcga agatgacgta gaagcgcgcg
    10081 gtgtcggcgc cgtagcggtc gatcagcgcc tggggatcca cgccgttgtt cttcgacttg
    10141 gacatggtgc cgatgccgtc gtacaccacc gcgcggccgt ctttcgcctg cgccgcgacg
    10201 atcttgccct tggcgtcgcg cgtcacttcg acctcggcgg ggttgatcca ctcgcgccgc
    10261 ccgcccgcct cgctgtagta ggactcggcg agcaccatgc cctgggtgag caggttggcg
    10321 aagggctcct ccacgccgag caggccctcg gccttcatgg cgcgcgtgaa gaacctcgag
    10381 tacaggaggt gcaggatggc gtgctcgatg ccgccgatgt actggtccac cggcatccag
    10441 tacttcgcgc gcgcatcgag catcgcgcgc tcgttgtccg ggcaggcgaa acgcgcgaag
    10501 taccaggacg aatcgacgaa ggtgtccatg gtgtcggttt cgcgcttcgc cggcttgccg
    10561 caggcggggc acctggtctc gtagaactcc ggcatcttgg cgagcgggtt gccggtgccg
    10621 tcgggcacca ggtgctcggg cagcttcacc gggagctgct cgtcgggcac cggcacgtcg
    10681 ccgcaggatg cgcagtgcac gatcgggatc gggcagcccc agtagcgctg gcgcgagatg
    10741 ccccagtcgc gcaggcgcca ttgcacctgc ttttcgccca ggcccttcgc cttcaggtcg
    10801 gcggcgatcg cgtcgacggc cgcctggtag tcgaggccgt cgtatttgcc ggaattgatg
    10861 caggtaccgt attcggcgta ctccggccgc cagggctcgg gcacgctgtc gccggccggc
    10921 gtgcggatga cgcacttgat cggcagggcg taggtcagcg cgaaggcgta gtcgcgctcg
    10981 tcgtgccccg gcacgcccat caccgcgccc tcgccgtagc ccatcagcac gtagttgccg
    11041 acccacaccg gcagcttctc gccggtgagc gggtggctgc agtacaggcc cgtcggcatg
    11101 cctttcttct ccagctgcgc gaggtcggcc tccatcactg cgcccttcct gcattgctcg
    11161 acgaaggcag cgagctttgc gtcgcgcttc gcggcgtgcg ccgcgatcgg gtgctcggcg
    11221 gccacggcca tgaaggtcgc gcccatcagg gtgtcggcgc gcgtggtgaa cacccagagc
    11281 gtttttttcg cgccgtccag ctcgtaggga aagccgatgc gcacgccctc ggagcgcccc
    11341 agccagttct tctgcatcag cttcacctgc tcgggccagc cgtccatctt ctccagcgcg
    11401 ccgaggagct cctcggcgta cgcggtgatg cggaagaagt acatcgggat ctcgcgcttt
    11461 tccaccggcg cgttggtgcg ccagcctttg ccgtcgatca cctgctcgtt ggcgagaacc
    11521 gtctggtccg ccgggtccca gttcacgacg ccggtcttct tgtaggcgag gcctttgcgg
    11581 aacaggcgcg tgaacagcca ctggttccac ttgtagtact cggggcggca ggtggcgaac
    11641 tcgcgctccc agtcgagggc gaagcccagc ccctgcatct ggcgcttcat ggtggcgatg
    11701 ttgtcgtagg tccacttcgc cggcggcacc ctgttggcca tcgccgcgtt ctccgccggc
    11761 aggccgaagg cgtcccagcc catcggctgc agcacgttca gcccgcgcat gcggtggtgg
    11821 cgcgtgagca cgtcgccgat cgtgtagttg cgcacgtgcc ccatgtgcag cttccccgag
    11881 gggtagggga acatggacag gcagtagaac ttcggcttgc ccggctcctc ccgcgagcgg
    11941 aacgattgcc gttgcgtcca gaaggcctgc gcctcgcgtt cgacctgctg gggttcgtat
    12001 tggggctgca tctgtgacga aatgcgcgga agccgcggat tctagcgcat ccacccccta
    12061 caattgcccg tataacccgg cgtaacgagt ccagggaggc cagcatgaaa cgcatactcg
    12121 gattgctcgc tgcggcggcg ctcgcgctag tcacgacgca ggggcttgcc gccgcggcgg
    12181 cggtggtgga aggcgtgcag atgcccgcct gggtggagcg cgcgagcgcc ggcgcgacgc
    12241 gccgcctgcc gctcgcgccg ggcatggaac tgcgcggcgg cgacgaagtg cgcaccggct
    12301 cgggctcgcg cctgtacatc aagctcgccg agggcagcct ggtgaagctg ggcgagaacg
    12361 cctcgctcaa gatcctcgac atcgcgcctg accgcggcgg ctttttcaag gcagcgctca
    12421 acgtgctgga gggcgccttt cgcttcacca ccgacgtgct cgccaaggag cggcgccgcg
    12481 acgtgagcat ccgcgtggcc accgtcactg ccggcatccg cggcaccgac ctgtggggca
    12541 agtcgacggc agagcgccag atcgtttgcc tgatcgaggg caagatcgag gtcggcgcgg
    12601 agggcgaggc gccggtgacc atggaccagg cgcgccagtt ctaccagcgc gacaagggcc
    12661 agacccagcc ggtggccttc gtcgacccgg tgcagctcgg gcagtgggcg cgcgagaccg
    12721 agatcgacga gggcaagggc gccgcgcgca gcggcggcaa gtggcgcgtg acgctggcaa
    12781 gcgtggagtc gcagtccgag gcgctcgcgg tgtacgacac gctgcgcacc gcgggcttcg
    12841 ctgcggagat ctttccgagc aagtcgggcg agaagcgcgt gtacctggtg cgcatctcgc
    12901 ggctcgcctc caaggccgac gccgaggcgc tcgccgtgca gctgcgcggc accgcgggcg
    12961 tgcaggagcc gaaggtttcg ggctaggcgg ctgcgttctt aattggtagg gtgggggcct
    13021 ccacggaggc cctcatgctt ttagcgcgac tggttgcggt actgcttgct tgctgtacga
    13081 cgctcgtcca ggcgcaggcc tggccttcga agcccgtgcg tatcgtggtc gcgttcgcgg
    13141 cgggcggctc gaccgacgtc gcggtgcgca gcatccagga gtcgctcgcg cgctcgttcg
    13201 ggcagccggt gatcgtggag aaccgcaccg gcgcggccgg caacatcgcc gcggagtacg
    13261 tcgtcaagca gccggcggac gggtacctgg tgctcgcgtc ggccgattcg ctcgcctcca
    13321 acccgcacct gttcaagaac ctgccgttcg atccggcgaa ggacctggtg ccggtggtgc
    13381 agctcacgcg ccagccggtg gtgctcgctg ttcacccttc cactggcgtg agcaacgtgg
    13441 ctgagttggt cgccgcggcg aaggcgaagc cgggaatggg ctacgccact tccggcgcgg
    13501 gctcgatcca gcacatggcg ggagagtggt tcgcgcagct cgcgggcatc aagctcacgc
    13561 acgtgcccta caagggcggt ggccaggcga tcacggacct cgtcggcggg caggtgccga
    13621 tcggctcgct cagcaatacg ccgct
//
LOCUS       DASRIE010000236         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1010987, whole genome shotgun sequence.
ACCESSION   DASRIE010000236 DASRIE010000000
VERSION     DASRIE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010987"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFB53_10100"
     CDS             complement(<1..284)
                     /locus_tag="VFB53_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87122.1"
                     /translation="MDFFEQQELSRRRTRWLILWFAVAVVATVASYCVAGGLVYALFT
                     LYATGRAGVPWEVPAAIAAVAGLCIAVVSACRLWQLREGGQAIAELLGARR"
     gene            complement(286..882)
                     /locus_tag="VFB53_10105"
     CDS             complement(286..882)
                     /locus_tag="VFB53_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN87123.1"
                     /translation="MGMGGWVFLAVVVGLAAWAIAVYNGLVALRNRFRNAFAQIDVQL
                     RRRYDLIPNLVESVKGYLQHERQTLEAVIQARGSAVSAAQRAAAAPGDPAAMHGLAQA
                     EGALGGALGRLLAVFESYPDLKANDNVLALQEELTSTENKVAFARQAYNDSVMQYNTK
                     RESFPDNVLAGAFGFAPAELLQATEHAEQRVAPKVSFR"
     gene            914..>2056
                     /locus_tag="VFB53_10110"
     CDS             914..>2056
                     /locus_tag="VFB53_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN87124.1"
                     /translation="MRSTLCRALRAAPHELPALAWAFTYFFLLLCGYYLLRPVRDALA
                     VDAGAQALQQLFTATFVTMLALVPVFGWLCAKLPRARLLPAVYAFFALNLAGFAIELH
                     PYVFFVWLSVFNLFAVSVFWSFMSDVFDTQQAARLYGVIAAGGSGGAIVGPLVAAAGA
                     ATLGTRGLLLLSAATLVAAIACIHRLVRWARAHPRAGDAPPEAPIGGSILAGARAALS
                     SPFLAAICGYLLCYTALSTALYFQQTEIVRQAVPEGAARIRLFAAVDLTVNSLALLMQ
                     LFAFSRLAALLSASWMLALMPIVSVAGFLWLGAAPTLLALVAFGVIRRVGEFAISKPA
                     REALFTLVPREDRYKAKNFIDTVVYRGGDALAARLLAGLPGVAFVAA"
BASE COUNT          258 a          738 c          690 g          370 t
ORIGIN      
        1 cggcgcgcgc ccagcagctc ggcaatggcc tggccgcctt cgcgcagctg ccagaggcgg
       61 caggccgata ccacggcgat gcacagcccg gcaacggcgg cgatcgccgc cgggacttcc
      121 cacggcacgc ccgcccgccc cgtggcgtag agggtaaaca gggcatagac gagcccgccg
      181 gcgacgcagt agctcgcgac cgtcgcgacg accgcaacgg cgaaccacag gatgagccag
      241 cgggtcctcc tgcggctcag ctcctgctgc tcgaagaaat ccaagctatc tgaacgaaac
      301 cttcggcgcg acgcgctgct ccgcgtgctc ggtcgcctgc agcagctccg ccggggcgaa
      361 accgaacgcg ccggccagga cgttgtccgg gaacgattcg cgcttggtgt tgtactgcat
      421 gaccgagtcg ttgtaggcct ggcgtgcgaa ggcgaccttg ttctccgtgc tggtgagctc
      481 ctcctggagc gcgaggacgt tgtcgttcgc cttgagatcg gggtagctct cgaacaccgc
      541 tagcagcctg ccgagcgcgc cgccgagcgc gccttcggcc tgggcgagcc cgtgcatcgc
      601 cgccgggtcg ccgggcgcgg ccgcggcacg ctgcgccgcg cttaccgcgc tgccgcgcgc
      661 ctggatcacc gcttcgagcg tctggcgctc gtgctgcagg tagcccttca cggattcgac
      721 gaggttcgga atgaggtcgt agcgccgccg cagctgcacg tcgatttgcg cgaaggcgtt
      781 cctgaagcgg ttacgcagcg ccaccaggcc gttgtagacg gcaatcgccc aggccgccaa
      841 gccgacgaca accgcgagga atacccagcc ccccattccc atgacgcccc cctttcgtgt
      901 cctactatag cgcatgcgaa gcaccctttg ccgggcgctg cgcgcagcgc cgcacgagct
      961 gccggcgctg gcgtgggcgt tcacatattt tttccttctg ttgtgcggtt actacttgct
     1021 gcggccggtg cgcgatgcgc tcgccgtgga cgccggcgcg caggcgctgc agcagctctt
     1081 cacggccacg ttcgtgacca tgctcgcgct cgtgccggtg ttcggctggc tgtgcgcgaa
     1141 gctgccgcgc gccaggctgc ttccggccgt ctacgcgttt ttcgcgctga acctcgcggg
     1201 attcgcgatc gagctgcacc cgtacgtgtt cttcgtctgg ctgagcgtct tcaacctctt
     1261 cgcggtgtcg gtgttctgga gcttcatgtc ggatgtgttc gacacccagc aggcggcgcg
     1321 cctgtacggc gtcatagccg ccggcggcag cggcggcgcg atcgtcggcc ccctggtcgc
     1381 cgcggcgggc gccgcgacgc tcggcacgcg cgggctgctt ctgctttccg ccgccacgct
     1441 cgtggcggcc atcgcctgca ttcaccggct ggtgcgctgg gcgcgcgcac atccgcgcgc
     1501 gggcgatgcg ccgcccgagg cgcctatcgg gggctcgatc cttgccggcg cccgcgcagc
     1561 gctcagctcg ccctttctcg ccgcgatctg cgggtatctg ctgtgctata cggcgctttc
     1621 cacggcgctc tacttccagc agaccgagat cgtgcggcaa gccgttccgg aaggcgccgc
     1681 gcgcatccgc ctcttcgccg ccgtggacct gaccgtcaat tcgctggcgc tgctgatgca
     1741 gcttttcgca ttctcgcggc ttgccgcgct gctttccgcg tcctggatgc tcgccctgat
     1801 gccgatcgtg tcggtggcgg gattcctctg gctcggcgcg gcgccgacac tgctggcgct
     1861 ggtggctttc ggcgtcatcc gccgcgtcgg agaattcgcg atctccaagc ccgcgcgcga
     1921 agcgctgttc acgctggtgc cgcgcgagga ccgctacaag gcgaagaact tcatcgatac
     1981 cgtggtctac cggggcggcg acgcgctcgc cgcgcgcctc ctcgcggggc ttccgggggt
     2041 ggccttcgtc gccgcc
//
LOCUS       DASRIE010000237        16612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2313770, whole genome shotgun sequence.
ACCESSION   DASRIE010000237 DASRIE010000000
VERSION     DASRIE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313770"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFB53_10115"
     CDS             complement(<1..226)
                     /locus_tag="VFB53_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87125.1"
                     /translation="MNRFFVTLGAAAFVFVSPVALGQCGGDTCSVGAAGTGGEMSGGK
                     AQGFREEFISVVRIPGAALTNSGNLDSGHIT"
     gene            332..1810
                     /locus_tag="VFB53_10120"
     CDS             332..1810
                     /locus_tag="VFB53_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN87126.1"
                     /translation="MNAPSRAGLPALPLKDPKLFREQCYLDGAWAGAAKTFPVHNPAS
                     GAPLGTVPDMGAAETKRAIAAAAAALPAWSARTGKERSAILRKWNDLILANLEDLALI
                     LTSEQGKPLAESKGEVAIGAAYIEWFAEEAKRIYGDTIPTPWADRRIVVVKQPVGVCA
                     AITPWNFPHSMITRKVAPALAAGCTVILKPAEQTPYSALALAELAERAGFPKGVLNIL
                     TGDAPAIGGELCANPTVRKISFTGSTEVGRLLMKQAAPTVKKISLELGGNAPFIVFDD
                     ADVDAAVEGAMVSKYRNTGQTCVCANRLFVQDGIYDKFAAKLAEKVKGLKVGAGTEAG
                     VTQGPLIDTNALAKVEEHVADALAGGAKALTGGKRHSLGRTYYEPTILTEAKPSMKIF
                     REETFGPVAPLFRFKTDAEVIELANRTEYGLAAYFYSRDIGRVWRVAEALEYGMVGVN
                     SGLITTEVAPFGGVKQSGLGREGSKYGCEEFVEVKYICMGGI"
     gene            complement(1807..2292)
                     /locus_tag="VFB53_10125"
     CDS             complement(1807..2292)
                     /locus_tag="VFB53_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN87127.1"
                     /translation="MPGADRNAGPRIGFGAARPADTPQLVELLGLLFTQEAELSPDAE
                     KQRRALQIILADPSRARIYVAREGEAILAMAALHFTISTAEGGKVAWFEDCIVRPEHR
                     RKGIGKALLEYVIGQAAAEGALRVMLLTDGDNATAQALYRKSGFRDSAMLAMRLKLRQ
                     R"
     gene            complement(2279..3010)
                     /locus_tag="VFB53_10130"
     CDS             complement(2279..3010)
                     /locus_tag="VFB53_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HZN87128.1"
                     /translation="MAMDREQLLREVASHSWFHTIDLGQGVVTPGRVDPQKQTLPRIG
                     LPADLSGKSVLDIGAWDGFFSFEAERRGARRVLATDSHAWWSCKPAFELARRALQSKV
                     EDFDIDVMDLAPDKIGTFDVVMMLGVLYHLRHPMLALEKVFGVTADHLILETVTDLTW
                     TSYPAMRFYPGDELARDSSNWFGPNPAAVMGMLKAVGFRKVELVSAKYRPLLYRIGRA
                     ILKRDMYKPFWPAIQNDRIVVHAWR"
     gene            complement(3016..4110)
                     /gene="ychF"
                     /locus_tag="VFB53_10135"
     CDS             complement(3016..4110)
                     /gene="ychF"
                     /locus_tag="VFB53_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZN87129.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAAIAAENYPFCTIEPNVGIV
                     EVPDGRLDRLAAIAKPLKVIPAAVEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFENPNVIHVANKVDPVSDIETINTELALADLETVGKQLSRNNKLAKTGGDKEAQ
                     RLVAVLEKAEKALDAGRPARSVDWSKEELAVMKPLFLLTMKPTMYVANVAENGFKDNP
                     LLAKVEAHARQEGAPVVAICAALEAQIADMSDADQQVFLADAGLSEPGLSRLARAAYA
                     LLGLQTYFTAGEKEVRAWTVVRGATAPQAAGVIHTDFERGFIRAEVAAYEDFVAHGGE
                     QGAKEAGKLRLEGKDYVVRDGDVMHFRFNV"
     gene            complement(4203..4829)
                     /gene="pth"
                     /locus_tag="VFB53_10140"
                     /pseudo
     CDS             complement(4203..4829)
                     /gene="pth"
                     /locus_tag="VFB53_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            complement(4842..5582)
                     /locus_tag="VFB53_10145"
     CDS             complement(4842..5582)
                     /locus_tag="VFB53_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZN87130.1"
                     /translation="MEIGANQRKVQGTGASRRLRRSGRVPGIVYGGDKEAVNIELDHK
                     ELYRNLTNEKFHASILTLKMESGAEQVLLRAFNMHPYKAQVQHVDFQRVSKDKKIHMK
                     VPLHFVNAANSPGVKDQAGVVSHVLNELDITCLPDDLPEFIEVDLINLSVGKSVHARD
                     LVLPKGVESTLHKGENPVVATCILPALIVEEEPATAEAVPAAADVPTTAQATPDKAAE
                     GAPPAAAGDKGKGADKAADKGGDKKDKK"
     gene            complement(5649..6593)
                     /locus_tag="VFB53_10150"
     CDS             complement(5649..6593)
                     /locus_tag="VFB53_10150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZN87131.1"
                     /translation="MDRLMIFAGNANTRLAQEVVKHLNLSMGRAIVGKFSDGEVMVEI
                     MENVRGKDVFVLQSTCAPTNDNLMELLVMVDALKRSSAARVTAAIPYFGYARQDRRPR
                     SARVAISAKVVANMLASVGVARVLTMDLHADQIQGFFDMPVDNVYAAPVLLGDVWKQR
                     YENLIVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANVSEVMNVIGEVKGRSCVIM
                     DDMVDTAGTLVKAAQVLKEEGATKVVAYCTHPVLSGAAVERIAASDLDELVVTDTIPL
                     RDDARACKKIRQLSVAGLLAETILRIFNEDSVSSLFIE"
     gene            complement(6644..6720)
                     /locus_tag="VFB53_10155"
     tRNA            complement(6644..6720)
                     /locus_tag="VFB53_10155"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6684..6686),aa:Gln,seq:ttg)
     gene            complement(6739..7566)
                     /gene="ispE"
                     /locus_tag="VFB53_10160"
     CDS             complement(6739..7566)
                     /gene="ispE"
                     /locus_tag="VFB53_10160"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZN87132.1"
                     /translation="MNWFPAPGKLNLFLHVLGRRADGFHLLQTVFRLIDRCDRVGIAV
                     REDGRLLRHDPLPAVSPAEDLCMRAAQLLRERCGTRLGADLAVHKQLPVGGGLGGGSS
                     DAATTLIVLNRLWEAGLSRGELQALAARLGADVPFFVFGENALGEGIGERLAPLQLPP
                     AWYVVLVPPVALSTQEVFSDNALTRNAKELKIPPFFSGMGTNDLEPAAIRRRPEIAAH
                     LAWLRTRRPQARMTGSGACVFAEFATEAEARAVLGELPATMSGFAAQGLHTHPLRGY"
     gene            complement(7563..8096)
                     /gene="lolB"
                     /locus_tag="VFB53_10165"
     CDS             complement(7563..8096)
                     /gene="lolB"
                     /locus_tag="VFB53_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HZN87133.1"
                     /translation="MNPATRVLALAATLLAACAELPQAPEGGFEFELNARFAARYRDE
                     AASGLLAWRHRAASDEVLLSSSFGQGLARISREGEKVTLLAGDDRRYSAADAETLTEQ
                     VLGFRLPLRGLADWVRARPAAGAPAQPVYAQDGRLQSLEQHGWRIEYLGYDGARPVRL
                     KLNYPGLELRLAIGEWK"
     gene            complement(8105..9841)
                     /locus_tag="VFB53_10170"
     CDS             complement(8105..9841)
                     /locus_tag="VFB53_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN87134.1"
                     /translation="MSAASRLLALAAALLLAGCAATAQNTPDAADAVDDDEPAQAAGT
                     PPDRLPAQDLSESLLYELLLAEVALQRGDAALAAQSYVELARRTKDPRVARRAVEVAN
                     AARAPQLALESARTWHEADPASLQALQTLTALLVSARQVEQAEPYLAQLLAKDGAAAA
                     NGFLQLSRLLGGNPDKQANLRLVRALAAKHPALPQARLAVAQAALAADDAQTALAEAR
                     RAAQLAPDWEAAAIFEAQVLQRKSPAQAGAALAAYLEKYPKAREARLSYARLLAGEKR
                     YAEARAEFEKLLAAHPNNGDVMYAVGLLALQLKDYATAEANLRRLLDTGFRDPNGVRF
                     TLGQAAEEQKKWDDAIGWYESIQRGEHFLPARMRAAGVMAKQGRLAEARKYLRAQGAE
                     GEQGVQLLIAEAQLLREANQHRDAFDLLGEALRAQPDQPELLYDHALTAEKLERFEIL
                     EGNLRKVIQIKPDHAHAYNALGYSFADRNLRLHEARKLIERALELSPQDYFIIDSMGW
                     VLYRMGDLKGAAAQLRRAYEGRPDGEIGAHLGEVLWMMGERAEAERIWLEALRNHAEN
                     ETLQKTVKRLRR"
     gene            9893..10702
                     /gene="mutM"
                     /locus_tag="VFB53_10175"
     CDS             9893..10702
                     /gene="mutM"
                     /locus_tag="VFB53_10175"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZN87135.1"
                     /translation="MPELPEVEVTRRGIAQKIAGRRISSVTVREARLRWPVTAAVRRL
                     AGRTVRSVARRAKYLLLDCGDGHLILHLGMSGSLRLLPSATAPEKHDHVDLVFGERML
                     RLRDPRRFGAVLWTTAQPAAHPLLRGLGVEPLSRALDGPQLHALTRGRRTAIKLFIMD
                     SRRVVGVGNIYASESLFGARIDPRRPAGRLSLEQCVALSNAIKRTLRAAIRAGGSSLR
                     DFVGSDGVYGCFQHRSRVYDRAGQPCRECGAPVRRIVQGQRSTYFCPSCQR"
     gene            complement(10699..11199)
                     /gene="coaD"
                     /locus_tag="VFB53_10180"
     CDS             complement(10699..11199)
                     /gene="coaD"
                     /locus_tag="VFB53_10180"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZN87136.1"
                     /translation="MIRAVYPGTFDPLTRGHEDLVRRAAKLFDTLILGVADSRAKRTF
                     FTLQERVEMAREVLGDLRNVQVVGFSGLLIEFIRQHDAHVVLRGLRAVSDFEYEFQLA
                     GMNRSLYPDFETMFLTPSEQHMFISATLVREIAVLGGDVAKFVHPVVGAKLAAKVRSL
                     GKSASA"
     gene            complement(11196..11726)
                     /gene="rsmD"
                     /locus_tag="VFB53_10185"
     CDS             complement(11196..11726)
                     /gene="rsmD"
                     /locus_tag="VFB53_10185"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZN87137.1"
                     /translation="MRIIAGEWRSRVIDVPARPGLRPTPDRVRETLFNWLGQRLDGCA
                     CLDLYAGSGALGFEAASRGAARVVLVESDRAALAALRRSRELLGAQRVEVVAGDAESF
                     LAGGGGRFDVVFLDPPFRQNALPALLGALPPRLAAGARVYLEAARPVAVPESWRELKR
                     ARAGQVSYQLLAWEPA"
     gene            11769..12686
                     /gene="ftsY"
                     /locus_tag="VFB53_10190"
     CDS             11769..12686
                     /gene="ftsY"
                     /locus_tag="VFB53_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZN87138.1"
                     /translation="MLAATSWAARLRNGLARTRAALGGGLGALFGRAAVDEALFEALE
                     SALLQADCGVAATRALLEALRARARRERIVEPQALKLVLQEELRALLAPLEKPLDVAR
                     ARPLVVMVAGVNGSGKTTSIGKLAKWLQGRGCSVLLAAGDTFRAAAREQLAAWGERNG
                     VAVIAQQSGDAAAVVYDAAAAARARRIDVLIADTAGRLPTQLHLMEEIKKVQRVLAKA
                     LPGAPHEVLLVLDANTGQNTLAQVKAFDAALGLTGLVLTKLDGTAKGGVVCAIAREHP
                     VPLLFVGVGEGIDDLRPFAAADFAEALLD"
     gene            12679..13332
                     /gene="ftsE"
                     /locus_tag="VFB53_10195"
     CDS             12679..13332
                     /gene="ftsE"
                     /locus_tag="VFB53_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZN87139.1"
                     /translation="MISFSAVSKRYPGGQDALRGVSFTLASGEFAFLTGHSGAGKSTL
                     LKLIPAIERPSAGSVIVNGQNIGALKRSALPYLRRNLGLVFQDQKLLFDRSVYDNVML
                     PLSLSGQPPREAARRVRAALDKVGLLARERANPVQLSGGEQQRLAIARAVVNRPALLV
                     ADEPTANLDAESAARIIDIFVAFHQVGVTLLIATHDQALIERYGKRVLRLSEGKLAA"
     gene            13329..14213
                     /gene="ftsX"
                     /locus_tag="VFB53_10200"
     CDS             13329..14213
                     /gene="ftsX"
                     /locus_tag="VFB53_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZN87140.1"
                     /translation="MIAWLRQHRQACAGALRKLAAQRAASLLNALVIGVALSLPAGGY
                     ALLANLQGVAQGFSFEPQLSLYLKAGVKRADLESRLRADARVARLRFVSREDALKELR
                     AAEGLADVVAALEHNPLPDAFVLRAVQAEPAALEALAAALRALPGVALVQVDSAWAQR
                     LAALGRIGRLALAVLALLLATGLVAVTFNTIRLQILTQREEIVVSQLLGATDAYVRRP
                     FFYLGLLQGLAGGLVALAILGASLAVLNPGVRELAQTYGSAFQLEFLGAADSLSIVAF
                     SGVLGWFGALLSVSKYLR"
     gene            14315..15103
                     /gene="rpoH"
                     /locus_tag="VFB53_10205"
                     /pseudo
     CDS             14315..15103
                     /gene="rpoH"
                     /locus_tag="VFB53_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
     gene            complement(15212..15757)
                     /locus_tag="VFB53_10210"
     CDS             complement(15212..15757)
                     /locus_tag="VFB53_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZN87141.1"
                     /translation="MLLLAACDGAQPKFKSTDITGADYGKQLELTDHRGKPRRLEEFR
                     GKAVVLFFGFTRCPDVCPTTLADAAQAMRQLGPDAERVQVLLVTVDPERDTGDVLAKY
                     VTAFDARFLGLRGDLDATRRAAKEFKVYFEKRPGKNPQDYSVDHSAQSYVLDPQGRLR
                     LFVRHDRIAEDLAPDLRLLLQ"
     gene            complement(15774..16610)
                     /gene="cyoE"
                     /locus_tag="VFB53_10215"
     CDS             complement(15774..16610)
                     /gene="cyoE"
                     /locus_tag="VFB53_10215"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN87142.1"
                     /translation="MTKPRVVSLIVFTAVIGMFLATPGMVPPRVLLAATLGIALVAGA
                     AAAINCLVEQNVDALMLRTRWRPLPSGELTNLQTLVFAGVVGGLGLWLLGHYVNALTL
                     WLTLATFVGYAIVYTVILKPLTPQNIVIGGASGAMPPVLGWAAVTGEVTTESMLLFLI
                     IFAWTPPHFWALALYRTEDYARAGLPMLPVTHGARYTRLQVLLYTLILFGVSLLPFAI
                     RMSGWLYLAAALALGGAFIYYALRLHLAYSDRLAQRAFRYSIVYLAALFGALLIDHYA
                     AL"
BASE COUNT         2499 a         5842 c         5685 g         2586 t
ORIGIN      
        1 ccgtgatgtg cccggagtcg aggttgccgc tgtttgtgag cgctgcaccg ggaatcctga
       61 cgacgctgat gaactcttcg cggaagcctt gagccttccc tccggacatt tcgccgccag
      121 taccggcggc gcctacgctg caggtgtcgc caccgcactg acccagcgca accggagaaa
      181 cgaaaacgaa cgcagccgca cccaatgtga cgaagaagcg gttcatggtc acctccaaaa
      241 ggcctcaaat cagtttgctc ccgccacgaa agcgggtcaa gggtgccgcc ttgttggtta
      301 gaattccatt ccgtacccag cgaggattcc catgaacgcc ccttcccgcg ccggcctgcc
      361 ggcgcttccc ctgaaggacc cgaagctgtt ccgcgaacag tgctacctcg acggcgcctg
      421 ggcgggcgcc gcgaagacct tcccggtgca caacccggcg agcggcgcgc cgctcggcac
      481 ggtgcccgac atgggcgccg cggagacgaa gcgggcgatc gccgccgccg ccgccgcgct
      541 gcccgcctgg agcgcgagga ccggcaagga gaggagcgca atcctgcgca aatggaacga
      601 cctgattctc gccaacctgg aggacctcgc gctgatcctc accagcgagc agggcaagcc
      661 gctcgccgaa tcgaaaggcg aggtcgccat cggcgcggcc tacatcgagt ggttcgccga
      721 agaagccaag cgcatctacg gcgacaccat cccgacgccc tgggccgatc gccgcatcgt
      781 ggtggtgaag cagccggtgg gcgtgtgcgc ggcgatcacg ccgtggaatt tcccgcactc
      841 gatgatcacg cgcaaagtgg cgccggcgct cgccgcgggc tgcaccgtga tcctcaagcc
      901 cgccgagcag acgccctact ccgcgctcgc gctggccgag ctcgccgagc gcgccggctt
      961 tccaaagggc gtgctcaaca tcctcaccgg cgacgcaccc gcgatcggcg gcgagctgtg
     1021 cgccaacccg acggtgcgca agatctcttt caccggctcg accgaggtcg ggcggctgct
     1081 gatgaagcag gcggccccga cggtgaagaa gatctcgctg gaactgggcg gcaacgcgcc
     1141 gttcatcgtg ttcgacgacg ccgacgtcga cgcggcggtg gagggcgcga tggtctccaa
     1201 gtaccgcaac accggacaga cctgcgtgtg cgccaaccgc ctgttcgtcc aggacggcat
     1261 ctatgacaag ttcgccgcga aactggcgga gaaggtgaag gggctgaagg tcggcgccgg
     1321 caccgaggcg ggcgtcacgc aaggcccgct catcgacacg aatgcgctgg cgaaggtcga
     1381 agagcacgtg gcggatgccc tcgccggcgg cgccaaggcg ctgaccggcg gcaagcgcca
     1441 ctcgctgggg cgcacctatt acgagccgac catcctcacc gaggcgaagc cctcgatgaa
     1501 gatcttccgc gaggagacct tcggcccggt ggcgcccctg ttccgcttca agaccgacgc
     1561 cgaggtgatc gagctcgcca accgcaccga gtacggcctg gcggcctact tctacagccg
     1621 cgacatcggc cgcgtatggc gcgtggccga ggcgctggaa tacggcatgg tgggcgtcaa
     1681 cagcggcttg atcaccacgg aagtggcgcc cttcggcggt gtgaagcagt ccgggcttgg
     1741 gcgcgagggc tccaagtacg gctgcgagga gttcgtcgag gtgaagtaca tctgcatggg
     1801 cggcatctag cgctggcgca gcttcagccg catcgccagc atcgccgagt cgcggaaacc
     1861 cgacttgcgg tagagcgcct gcgcggtggc gttgtcgccg tcggtgagca gcatcacgcg
     1921 cagcgcgcct tcggctgcgg cctgcccgat cacgtactcg agcagcgcct tgccgatgcc
     1981 cttgcgccga tgctccgggc gcacgatgca gtcctcgaac caggcgacct tgccgccttc
     2041 ggcagtgctg atggtgaagt gcagcgccgc catggcgagg atcgcctcgc cctcccgcgc
     2101 cacatagatg cgggcacgcg aaggatccgc gaggatgatc tgcaacgcac gacgctgctt
     2161 ttccgcgtcg ggggagagct cggcttcctg ggtaaagagg aggcccagca gttcgacgag
     2221 ctgcggcgtg tcggcaggcc gcgccgcgcc gaatccgatc cgcggccccg cgttccggtc
     2281 agcgccaggc atgcaccacg atgcggtcgt tctggatcgc gggccagaac ggcttgtaca
     2341 tgtcgcgctt caggatcgcc cgtccgatgc ggtaaaggag cggacggtac ttcgccgaca
     2401 cgagctccac cttcctgaaa cccacggcct tcagcatgcc catcacggca gcgggattcg
     2461 gtccgaacca gttgctgctg tcgcgcgcca gttcatcccc gggatagaag cgcatcgccg
     2521 gataggaagt ccaggtaagg tcggtgaccg tctcgaggat caggtgatcg gcggtgacgc
     2581 cgaatacctt ctccaaggca agcatggggt ggcgcaggtg gtacagcacc cccagcatca
     2641 tgaccacgtc gaaggtcccg atcttgtcgg gggcgagatc catgacgtcg atatcgaaat
     2701 cctcgacctt ggactgcagc gcgcggcgcg ccagctcgaa ggccggtttg cagctccacc
     2761 aggcatggga gtcggtcgcc agcacgcgcc tcgcgccgcg ccgctctgcc tcgaacgaga
     2821 aaaaaccgtc ccaggcgccg atatccagga ccgacttgcc gctcagatcg gcgggcaggc
     2881 cgatgcgcgg caaggtctgt ttctgcggat cgacgcgccc gggcgttacg acgccctgcc
     2941 ccaggtcgat ggtatggaac cagctgtggc ttgcaacttc ccgcagcagt tgctctcgat
     3001 ccatggccat ggagcctaga cgttgaagcg aaagtgcatg acgtcgccgt cgcgcaccac
     3061 gtagtccttg ccttcgaggc gcagcttgcc ggcttccttg gcgccctgct cgccgccgtg
     3121 ggcgacgaag tcctcgtagg cggcgacctc ggcgcggatg aagccgcgct cgaaatcggt
     3181 atggatcacc cccgccgcct gcggcgcggt ggcgccccgc accacggtcc aggcgcgcac
     3241 ttccttctcg ccggcggtga aataggtctg caggccgagc agggcatagg cggcgcgcgc
     3301 gaggcggctc aggcccggct cgctcaatcc cgcgtcggcg agaaacacct gctggtccgc
     3361 gtcgctcatg tccgcgatct gcgcttccag cgccgcgcag atggcgacca ccggcgcgcc
     3421 ttcctgcctg gcgtgcgcct cgaccttggc cagcagaggg ttgtccttga agccgttctc
     3481 cgccacgttg gcgacgtaca tggtcggctt catggtgagg aggaacagag gcttcatcac
     3541 cgccagttcc tccttcgacc agtcgaccga gcgcgccggg cgccccgcgt cgagcgcctt
     3601 ctcggccttc tccagcacgg cgaccaggcg ctgcgcctcc ttgtcgccgc cggtcttggc
     3661 gagcttgttg ttcctcgaca gctgcttgcc caccgtctcc aggtcggcga gcgccagctc
     3721 ggtgttgatg gtctcgatgt cggagaccgg atcgaccttg ttggcgacgt ggatcacgtt
     3781 cggattctcg aagcagcgca ccacgtgcgc gatggcgtcg gtctcgcgga tgttggcgag
     3841 gaacttgttg cccaggcctt cgcctttgga ggcgcccgcc accagcccgg cgatgtcgac
     3901 gaactccacc gccgccggaa tcaccttcaa cggcttggcg atcgcggcca gcctgtccag
     3961 ccggccatcc ggcacctcga cgatgccgac gttcggctcg atggtgcaga agggatagtt
     4021 ctcggcggcg atggccgcct tggtgagcgc attgaacagc gtggacttgc cgacgttcgg
     4081 cagtcccacg atgccgcact tcaagctcat ttcttcgagc ccgtcaggcc cttgaagaat
     4141 cccctcaggc cgcccgtctt ttcttccttc ggaggctcgg atttcttcgg ctccggcttc
     4201 ttcgcttccg gtttcttggc ggccggtttc ttcgcgtccg gcgtcttcgc ctccgtctgc
     4261 ttcgccacca ccggcttgtc ctggctgtgc agcttgttca tggcgccctg cgcgtcgccc
     4321 tccagcatct gcggcagcac ctcgagcgcg cgggcgatcg aaccctcgat cgcctcgcgt
     4381 tcctccgccg gcggcttttc cagcacgtag tccgcgccgt ccctgcccgc cggccggccc
     4441 acgccgatgc gcagccgcca gtagtcgtgc gaacccaggc gctgcgagat gtcgcgcaga
     4501 ccattgtgtc cggagatgcc gccgccctgc ttgatgcgcg cggtgcccgc cgggaagtcg
     4561 agctcgtcgt gcaccaccag gatttcctcc gccgggatct tgaagaaccc ggcgagcatc
     4621 tgcacggcgc gaccggaggc gttcatgaag gtctgcggca gcaccagcca caggccggcg
     4681 tcgcgcgcca ccagcgcctg gtagtggttg tccttcttca gcgccatgcc gcgggccagg
     4741 gcaaggcgct cgacgagcca gaacccggcg ttgtgccggg tgcgctcgta ctccttgccc
     4801 gggttgccca ggccgacgat gaggcggatc ttggccagcg gctacttctt gtcttttttg
     4861 tcgccgccct tgtcggcagc cttgtccgcg cccttgccct tgtcgcccgc ggcggcgggc
     4921 ggcgcgccct cggccgcctt gtcgggcgtc gcctgcgccg tggtcgggac gtcggctgcg
     4981 gccggcacag cctccgccgt ggcgggttcc tcctcgacga tcagcgccgg caggatgcag
     5041 gtggcgacca ccggattctc gcccttgtgc agcgtcgact cgacgccctt tggcagcacc
     5101 aggtccctgg catgcaccga cttgccgacc gacaggttga tcaggtccac ctcgatgaac
     5161 tccggcaggt cgtccggcag gcaggtgatg tcgagttcgt tgagcacgtg gctcaccacc
     5221 ccggcctggt ccttcacccc cggcgaattc gccgcgttga cgaagtgcag cggcaccttc
     5281 atgtggatct tcttgtcctt ggagacgcgc tggaagtcca cgtgctgcac ctgcgccttg
     5341 tacggatgca tgttgaaggc gcgcagcagc acctgctcgg cgccgctttc catcttcagc
     5401 gtcaggatcg aggcgtggaa cttctcgttg gtcaggttgc ggtacagctc cttgtggtcg
     5461 agctcgatgt tgaccgcctc cttgtctccg ccgtagacga tgcccgggac gcgtcccgaa
     5521 cggcgcagac ggcggctcgc acccgtccct tgcaccttgc gctggttagc gccgatttcg
     5581 attttcatgc acagctcctc gttgatccgc cgggcccgcg accaggcaca ggcgggattg
     5641 gaaaaacctc attcgatgaa cagcgaactg accgaatcct cgttgaagat gcgcaggatc
     5701 gtctccgcca gcagccccgc cacggacagc tgccggatct tcttgcaggc gcgcgcgtcg
     5761 tcgcgcagcg ggatggtgtc ggtgaccacc agttcgtcca ggtcggaggc ggcgatgcgc
     5821 tccaccgccg cgcccgagag caccggatgc gtgcaatagg ccaccacttt ggtggcgccc
     5881 tcttccttca gcacctgcgc cgccttcacc agcgtgcccg cggtgtccac catgtcgtcc
     5941 atgatcacgc agctgcggcc cttcacctcg ccgatcacgt tcatcacttc cgagacgttg
     6001 gccttcggcc ggcgcttgtc gatgatcgcc aggtccgatt cgagccgctt cgcgagcgcg
     6061 cgcgcgcgca ccacgccgcc cacgtcgggc gagaccacga tcaggttctc gtagcgctgc
     6121 ttccacacgt cgcccagcag caccggcgcc gcatacacgt tgtccacggg catgtcgaag
     6181 aagccctgga tctggtcggc gtgcaggtcc atggtcagca cgcgcgccac gcccaccgag
     6241 gcgagcatgt tggccaccac cttggcgctg atggcgacgc gcgccgagcg cgggcgccgg
     6301 tcctggcgcg catagccgaa atagggaatg gcggcggtga cgcgcgcggc cgaggagcgc
     6361 ttgagcgcat ccaccatcac cagcagttcc atcaggttgt cgttggtcgg cgcgcaggtg
     6421 gactgcagca cgaacacgtc cttgccacgc acgttctcca tgatctcgac catcacctcg
     6481 ccgtcggaga acttgccgac gatggcgcgc cccatcgaca ggttgaggtg cttcaccacc
     6541 tcctgcgcca gccgcgtgtt ggcgttgccg gcgaagatca tcaggcggtc catgagggag
     6601 gcgatccggt tcatggcgtc aggcagcggc caccgcgggc aaatggctgg ggaggaagga
     6661 ttcgaaccct cgaatggcgg aatcaaaatc cgctgcctta accaacttgg cgactcccca
     6721 ttggatcaag tccatagatc agtatccgcg cagcgggtgc gtgtgtagcc cctgcgcggc
     6781 gaatccgctc atcgtggcag gcaattcgcc cagcaccgcg cgcgcttctg cttccgttgc
     6841 aaattccgcg aacacgcaag cccccgaacc ggtcatccgc gcctgcggcc ggcgcgtccg
     6901 cagccacgcc aggtgcgccg cgatctcggg gcggcgcctg attgccgccg gttcgagatc
     6961 gttcgttccc atgccggaga aaaagggcgg tattttgagt tctttcgcat tacgtgtcaa
     7021 cgcgttatcg ctgaaaacct cctgcgtgga aagcgccacg ggcggcacca gcaccacata
     7081 ccaggcgggc ggcagctgca gcggcgccag gcgctcgcca atgccctcgc ccagcgcgtt
     7141 ctcgccgaac acgaagaacg gcacgtccgc gccaaggcgg gccgccagcg cctgcagttc
     7201 gccgcgggac aggccggcct cccagaggcg gttcaggacg atgagcgtgg tggcggcgtc
     7261 cgacgagccg ccgcccaggc cgccacccac cggcagctgc ttgtggacgg caaggtcggc
     7321 gcccaggcgc gtgccgcagc gctcgcgcag caattgcgcg gcgcgcatgc acaggtcctc
     7381 cgccggcgac acggcgggca gcgggtcgtg gcgcaggagc ctgccgtctt cgcgcacggc
     7441 gatgccgacc cggtcgcagc ggtcgatcag gcggaatacc gtctgcaaca ggtggaagcc
     7501 gtcggcgcgg cggccgagca cgtgcaggaa caggttcaac ttcccgggcg cgggaaacca
     7561 gttcatttcc actcgccgat ggccaggcgc agctccagcc ccgggtagtt gagcttgagc
     7621 cgcaccgggc gcgcgccgtc gtaacccagg tattcgatgc gccagccgtg ctgctcgagc
     7681 gactgcaggc ggccgtcctg cgcgtagacc ggctgcgccg gcgcgccggc cgccggccgc
     7741 gcgcgcaccc agtcggcaag gccgcgcagc ggcaggcgga agccgagcac ctgctcggtg
     7801 agcgtttcgg cgtcggcggc ggagtagcgc cggtcgtcgc cggccagcag ggtgaccttc
     7861 tcgccctcgc gcgagatgcg cgccaggccc tgcccgaagg aggaactcag cagcacctcg
     7921 tccgaggcgg cgcggtggcg ccaggcgagc aggccggagg ccgcctcatc gcggtagcgc
     7981 gccgcgaagc gcgcgttgag ctcgaactcg aagccgccct cgggcgcctg gggcaactcg
     8041 gcgcaggcgg cgagcagggt cgctgccagt gccaggacgc gcgtcgcggg gttcatgcgg
     8101 cccgtcagcg gcgcaggcgc ttgaccgtct tctgcagggt ctcgttctcg gcgtggttcc
     8161 tgagcgcctc gagccagatg cgttcggcct cggcgcgctc gcccatcatc cacagcactt
     8221 cgcccaggtg ggcgccgatt tcgccgtccg ggcgcccctc gtacgcgcgc cgcagctgcg
     8281 ccgccgcgcc cttgaggtcg cccatgcggt agagcaccca gcccatgctg tcgatgatga
     8341 agtaatcctg cggcgagagc tcgagcgcgc gttcgatgag cttgcgcgcc tcgtgcaggc
     8401 gcaggttgcg gtcggcgaaa gagtagccga gcgcattgta ggcgtgcgca tggtccggct
     8461 tgatctggat caccttgcgc aggttgcctt ccaggatctc gaagcgctcg agcttctccg
     8521 cggtgagcgc atggtcgtac agcagctccg gctgatcggg ctgcgcgcgc agtgcctcgc
     8581 ccagcaggtc gaaggcgtcg cggtgctggt tggcctcgcg cagcagctgc gcttcggcga
     8641 tcagcagctg cacgccttgc tcaccctccg cgccctgcgc gcgcaggtac ttgcgcgcct
     8701 ccgccagccg gccctgcttg gccatcaccc cggcggcgcg catgcgcgcc ggcagaaagt
     8761 gctcgccccg ctggatgctc tcgtaccagc cgatggcgtc gtcccacttc ttctgctcct
     8821 ccgcggcctg gccgagcgtg aagcgcacgc cgttcgggtc gcggaagccc gtgtcgagca
     8881 gccggcgcag attcgcctcg gcggtggcgt aatccttgag ctgcagggcg agcaggccga
     8941 ccgcgtacat cacgtcgccg ttgttcgggt gggcggcgag cagcttctcg aactcggcgc
     9001 gcgcctcggc gtacctcttc tcgcccgcca gcaggcgcgc atagctgagc cgcgcctcgc
     9061 gcgccttcgg gtatttctcc aggtacgccg cgagcgcggc gcccgcctgc gcgggcgact
     9121 tgcgctgcag cacctgcgct tcgaagatcg ccgccgcttc ccagtcgggg gccagttgcg
     9181 cggcgcggcg cgcctcggcg agcgccgtct gcgcatcgtc cgcggccagc gccgcctgcg
     9241 ccaccgctag gcgggcctgc ggcagtgcgg ggtgcttcgc cgccagcgcg cgcaccagcc
     9301 gcagattggc ctgcttgtcg gggttgccgc cgagcagccg cgagagctgc aggaaaccgt
     9361 tcgcggcggc ggcgccgtcc ttcgccagca gttgggcgag gtagggttcg gcctgctcca
     9421 cctggcgagc gctcaccagc agcgcggtga gcgtctgcag cgcctgcagc gaagcggggt
     9481 ccgcctcgtg ccaggtgcgc gccgactcca gcgccagctg cggcgcgcgc gcggcattgg
     9541 ccacctcgac cgcgcgacgc gcgacgcgcg ggtccttggt gcgcctggca agctcgacgt
     9601 aggactgcgc ggcgagcgcc gcatcgccgc gctgcagcgc gacctcggca agcagcaact
     9661 cgtacagcag cgactccgac aggtcctgcg ccggcaggcg gtcgggcggc gtgccggcgg
     9721 cctgcgccgg ctcgtcgtcg tcgacggcgt cggcagcgtc gggggtgttc tgcgcggtcg
     9781 cggcgcagcc ggcgaggagc agcgcggcgg cgagcgcaag caggcgggat gcagcggaca
     9841 tggcgtctgg agtgtaggta tatttggtgc cccgcacaag ccaatggttc ccgtgccaga
     9901 gctaccggaa gtcgaagtaa cccgccgcgg catcgcgcag aaaatcgccg ggcgcaggat
     9961 ctcctcggtc acagtgcgcg aggcgcgact gcgctggccg gtgactgcgg ccgtgcggcg
    10021 ccttgcgggc cgcaccgtgc gctcggtggc gcggcgcgcc aagtacctgc tgctcgattg
    10081 cggcgacggg cacctgatcc tgcacctggg aatgagcggc agcttgcgcc tgctgccaag
    10141 cgccaccgcg ccggaaaaac acgatcacgt cgatctggtg ttcggcgagc gcatgctgcg
    10201 gctgcgcgac ccgcggcgct tcggtgccgt gctgtggacc acggcgcagc cggccgcgca
    10261 cccgctgctg cgcggactcg gcgtcgaacc gctgtcgcgt gcgctcgatg ggccgcagct
    10321 gcacgcgctc acgcgcggcc gccgcacggc gatcaagctg ttcatcatgg acagccggcg
    10381 cgtggtcggc gtcggcaaca tctacgccag cgagagcctg ttcggcgcgc gcatcgaccc
    10441 gcgccggccg gcggggcggc tgtcgctcga gcagtgcgta gcgctgtcga acgcgatcaa
    10501 gcgcacgctg cgcgcggcga tccgcgccgg cggctcgagc ttgcgcgatt tcgtcggcag
    10561 cgacggcgtc tacggctgct tccagcatcg ctcccgggtc tatgaccggg cgggacagcc
    10621 ctgccgcgaa tgcggcgcgc cggtgcgccg catcgtgcag gggcagcgct cgacctactt
    10681 ctgcccgtcc tgccagcgct aggcgctggc ggacttgccg agcgagcgca ccttggcggc
    10741 aagcttggcg ccgaccaccg ggtgcacgaa cttggcgacg tcgccgccga gcacggcgat
    10801 ctcgcgcacc agggtcgccg aaatgaacat gtgctgctcc gagggcgtga ggaacatggt
    10861 ctcgaagtcc gggtacaggc tgcggttcat cccggccagc tggaactcgt attcgaagtc
    10921 ggacaccgcg cgcagcccgc gcagcaccac gtgcgcgtcg tgctggcgga tgaactcgat
    10981 cagcaggccg ctgaagccca ccacctggac gttcctcagg tcgccgagca cctcgcgcgc
    11041 catctcgacg cgctcctgca gagtgaagaa cgtgcgcttg gccctgctgt cggcgacccc
    11101 caggatcagc gtgtcgaaga gcttggccgc gcgccgcacc aagtcctcgt ggccgcgggt
    11161 cagcggatcg aaagtgccgg ggtacaccgc tcggatcatg ctggctccca tgcaagcagt
    11221 tggtagctga cctgccccgc gcgcgcgcgc ttcagctcgc gccaggactc tggaaccgcg
    11281 accggccgcg ccgcctccag gtagacgcgc gcgccggcgg caaggcgcgg cggcaatgcg
    11341 ccgagcagcg ccggcagggc attctgcctg aacggcgggt cgaggaaaac cacgtcgaag
    11401 cgcccgccgc cgcccgccag gaacgactcc gcatcgcccg ccaccacctc gacgcgctgc
    11461 gcgcccagca actcgcggct gcggcgcagc gccgcgagcg ccgcgcggtc gctctcgacc
    11521 agcaccacgc gcgccgcgcc gcgcgaggcg gcctcgaagc cgagcgcgcc gctgccggcg
    11581 tacagatcca ggcaggcgca gccgtccagg cgctggccga gccagttgaa cagcgtctcg
    11641 cgcacgcgat ccggcgtcgg ccgcagcccg gggcgggccg gcacatcgat gacgcggctg
    11701 cgccactcgc cggcgatgat gcgcacgcgt ccggcgggac ggctacgctt gctagaatcc
    11761 acgcgaccat gttagcagcg acctcgtggg cagcgcgcct caggaacggc ctcgcgcgca
    11821 cgcgcgccgc gctcggcggc gggctgggcg cgctgttcgg ccgcgccgcg gtggacgagg
    11881 cgctgttcga ggcgctcgag tccgccctgc tgcaggccga ttgcggcgtg gcggcaacgc
    11941 gcgcgctcct cgaggcgctg cgcgcacggg cgcgccgcga gcgcatcgtc gagccgcagg
    12001 cgctcaagct cgtgctgcag gaagagttgc gcgcgctgct cgcgccgctg gaaaaaccgc
    12061 tcgatgtcgc gcgcgcccgg ccgctggtgg tgatggtggc gggggtcaac ggctcgggca
    12121 agaccacctc gatcggcaag ctggcgaaat ggctgcaagg ccggggctgc agcgtgctgc
    12181 tcgccgccgg cgacacgttc cgcgcggcgg cgcgcgaaca gctcgccgcc tggggcgaga
    12241 ggaacggcgt ggcggtgatc gcgcagcaat ccggcgacgc cgcggcggtg gtgtacgacg
    12301 cggcggcagc ggcgcgcgcg cgccgcatcg acgtgctgat cgccgacacc gccgggcgcc
    12361 tgccgacgca gttgcacctg atggaggaga tcaagaaggt ccagcgcgtg ctggccaagg
    12421 cgctgcccgg cgcgccgcac gaagtgctgc tggtgctgga cgcgaacacc gggcagaaca
    12481 cgctcgccca ggtcaaggcc ttcgacgcgg cgctcggcct caccgggctg gtgctcacca
    12541 agctcgacgg caccgccaag ggcggcgtgg tgtgcgccat cgcgcgcgag cacccggtgc
    12601 cgctgctgtt cgtcggggtg ggcgaaggca tcgacgacct gcggccgttc gcggcggccg
    12661 atttcgcgga ggcgctgctt gattagcttc tcggcggtct ccaagcgcta tccgggaggg
    12721 caggacgctc tgcgcggcgt gtctttcacg ctggcctcgg gcgagttcgc cttcctcacc
    12781 ggccactccg gcgcgggcaa gtcgacgctg ctcaagctga tcccggcgat cgagcggccg
    12841 agcgccggca gcgtgatcgt caatggccag aacatcggcg ccctcaagcg ctccgcgctt
    12901 ccctacctgc ggcgcaacct cggcctggtg ttccaggacc agaagctgct tttcgaccgc
    12961 tccgtgtacg acaacgtgat gctgccgctg tcgctcagcg ggcagccgcc ccgcgaagcg
    13021 gcgcgccgcg tgcgcgccgc gctcgacaag gtggggctgc tcgcgcgcga gcgcgccaac
    13081 ccggtgcagc tctccggcgg cgagcagcag cgcctggcga tcgcgcgggc ggtggtcaac
    13141 cgcccggcgc ttctggtcgc cgacgaacca accgccaacc tggatgcgga atcggcggcg
    13201 cgcatcatcg acatcttcgt cgccttccac caggtcggcg tcacgctgct catcgccacg
    13261 cacgaccagg cgctcatcga gcgctacggc aagcgcgtgc tgcgcctgag cgagggaaag
    13321 ctggccgcgt gatcgcctgg ctgcgccagc accgccaggc ctgcgccggt gcgctgcgca
    13381 agctcgcggc gcagcgtgcc gcgagcctgc tcaatgccct ggtgatcggc gtggcgctgt
    13441 ccctgcccgc gggcggctac gccctgctcg ccaatctgca gggcgtggcg cagggcttca
    13501 gcttcgagcc gcagctgtcg ctgtacttga aggcgggagt gaagcgcgcc gatctcgaat
    13561 cccggctgcg cgccgacgcc cgggtcgcgc gactgcgttt cgtatcgcgc gaggacgcgc
    13621 tcaaggaact gcgcgctgcc gaggggctcg ccgacgtggt cgcggcgctg gagcacaatc
    13681 ccctgcccga cgcgttcgtg ctgcgcgccg tgcaggccga acccgccgcc ctcgaggcgc
    13741 tggccgcggc gctgcgcgcg ttgccgggcg tggccctggt gcaggtggat tccgcctggg
    13801 cgcagcggct tgccgcgctg gggcgcatcg ggcgcctcgc gctcgccgtc ctcgccctgc
    13861 tgctcgccac cggtctggtc gcggtcacct tcaataccat ccgcctgcag atcctcaccc
    13921 agcgtgaaga gatcgtggtg tcccagctgc tcggggccac cgacgcctac gtgcgccggc
    13981 cgttcttcta tctgggcctg ctgcagggac tcgcgggcgg cctggtggca ttggccatcc
    14041 tgggagcgag cctggcggtg ctcaatcccg gggtgcgcga gctggcgcag acctacggat
    14101 cggcattcca gctggaattt ctcggcgccg ccgattcgct ttccatcgtg gccttctccg
    14161 gggtcctggg ttggttcgga gcgcttctat cagtaagtaa atacttacga taaaaccctt
    14221 gtttcgcata tctgtttaca cgaagtggtc gggaacctcc agggccatta gcactccaat
    14281 acccggagtg ctaagatttc ccagtggagg tgcaatgacg aataccacgc aggccctgcc
    14341 gatggcgctg cccctgccga tggccgcggg cagcctcgag gcgtacatcc agtcggtcag
    14401 ccgcttcccg ctgctcagcg ccgagcagga gcgcgagtac gcgctgcgtt accgccgcga
    14461 cggcgacgtc gacgcggcgc gccagctggt gctgtcgcac ctgcggctgg tggtcgcggt
    14521 cgcccgcggc tacctcggct acggcctgcc gcatgccgat ctcattcagg aaggcaacct
    14581 cggcctgatg aaggcagtga agcgcttcga ccccgcgcgc ggcgtgcgtc tggtgtcgtt
    14641 cgcgatccat tgggtgcggg ccgagatcca tgagtacgtc ctgcgcaact ggcgcctggt
    14701 gaaaatcgcg accaccaagg cgcagaggaa actgttcttc aacctgcgct cgatgcgcca
    14761 gggcctcgcc ccgctgagcg ccgccgaagt caggcgcgtc gccgccgagc tgaaggtcaa
    14821 gcccgaggaa gtgatggaga tggagacgcg gctgggcggc cgcgacatcg ccttcgaggc
    14881 cgaggacagc gacgaggagg cctacgcgcc ggtgcattac ctggcggccg acccgcgggc
    14941 cgaaccctcc gccgtgctgc aggagcagca agaccaggac gcggacgccg ccggcctgcg
    15001 gcgcgcgatg gcgagcctgg acgcgcgcag ccggcgcatt gtcgaggcgc gctggctgaa
    15061 ggaacaggac tccgccacgc tgcacgacct ggccgccgag ttctaggtgt ccgccgaacg
    15121 catccggcag atcgaggcga aggcgctgca gaagatgaag cagctgatgc aggcgccgcc
    15181 cgccctgccc gcgcccgcgg cgcgctgagc cctactgcag cagcagccgc aggtccgggg
    15241 ccaggtcctc ggcgatgcgg tcgtgccgca cgaacaggcg caggcgcccc tgcgggtcga
    15301 gcacgtagct ctgggcgctg tggtcgacgg aatagtcctg cgggttcttg cccggccgct
    15361 tctcgaaata cactttgaat tctttcgccg cgcgccgcgt ggcgtccagg tcgccccgca
    15421 ggccgaggaa gcgcgcgtcg aacgccgtca cgtatttcgc cagcacgtcc ccggtatcgc
    15481 gctccgggtc cacggtcacc agcagcacct gcacgcgctc ggcgtcgggc ccgagctggc
    15541 gcatcgcctg cgcggcgtcg gccagcgtcg tcgggcagac gtcgggacag cgcgtgaagc
    15601 cgaagaacag caccaccgcc ttgccgcgga attcctccag gcggcgcggc ttgccgcggt
    15661 ggtcggtgag ctccagctgc ttgccgtaat ccgcaccggt aatgtcggtg ctcttgaatt
    15721 tcggctgcgc gccgtcgcag gccgcgagca gcagcatgcc cagggccgcc aggctaaagc
    15781 gcagcgtagt ggtcaatcag cagcgccccg aacaacgcgg cgaggtagac gatcgaatag
    15841 cggaacgccc gctgcgccag ccggtcgctg taggcgaggt gcaggcgcag tgcgtaatag
    15901 atgaacgcgc cgcccagcgc cagcgccgcg gcgagataca gccacccgct catgcggatg
    15961 gcgaacggca gcagcgagac gccgaacagg atcagcgtgt agagcagcac ctgcagccgc
    16021 gtgtagcgcg cgccgtgcgt caccggcagc atcggcagcc ccgccctggc gtagtcctcg
    16081 gtccggtaca gggccagcgc ccagaaatgc ggcggcgtcc aggcgaagat gatgaggaac
    16141 agcagcatcg actcggtcgt cacctcgccg gtcactgccg cccagcccag caccggcggc
    16201 atggccccgg atgccccgcc gatgacgatg ttctgcgggg tgagcggctt gaggatcacc
    16261 gtgtagacga tggcgtaacc gacgaaggtg gccagcgtca gccacagggt gagcgcgttc
    16321 acgtagtggc ccaggagcca caggcccagc ccgccgacca cgccggcgaa caccagcgtc
    16381 tgcaggttgg tgagctcgcc gctcggcagc ggccgccagc gcgtgcgcag catcagcgca
    16441 tccacgttct gctccaccag gcagttgatc gccgccgccg cgccggccac cagcgcgatg
    16501 ccgagcgtgg cggcgagcag cacccgcggc ggcaccatcc ccggcgtggc gaggaacatg
    16561 ccgatcaccg cggtgaacac gatcagcgac accacgcgcg gcttggtcag cg
//
LOCUS       DASRIE010000238         5313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2575844, whole genome shotgun sequence.
ACCESSION   DASRIE010000238 DASRIE010000000
VERSION     DASRIE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575844"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..728)
                     /locus_tag="VFB53_10220"
     CDS             complement(102..728)
                     /locus_tag="VFB53_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87143.1"
                     /translation="MPAELFQAAHLLLVYLHLLATCAALGAILVSDLHVLGKMRRGGG
                     RLLPPEPMVRDVVAWSLLALVVTGGGLVAAGIARDPSYLTGNPKLMAKIVLVALLAVN
                     AVVLHRLTFPRLETKRPLCMTAGDDVVGVAVPAALSTTLWFFAAFLGIARPWNRVMPM
                     ENVLGIAGGLFVALLVLAVVLLAMTERRSPARRPGQAAAGATADAVEA"
     gene            808..2088
                     /locus_tag="VFB53_10225"
     CDS             808..2088
                     /locus_tag="VFB53_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN87144.1"
                     /translation="MLDLLFRDVALPDGRRDLDVHVRDGRIEAIGARLHAPEGADVVA
                     GGGQLLAPSFVDAHFHLDATLSYGLPRVNASGTLLEGIALWGELKPALTQEALVQRAL
                     AYCDWAVAKGLLAIRSHVDVCDDRLLAVEALLDVKRRVAPYLDLQLVAFPQDGVLRSP
                     GALANLKRALAMGVDVVGGIPHFERTMAEGAESVRLLCELAAEQGRRVDLHCDESDDP
                     LSRHIETLAFHTQRLGLQGRVTGSHLTSMHSMDNYYVSKLLPLMAEAGVHAVANPLVN
                     ITLQGRHDTYPRRRGMTRVPELLAAGVNVAFGHDCVMDPWYGLGSADMLEVAHMGLHV
                     AQMTSQAQMKACFDAVTVNAARVLGLDDHGVAVGRSADLVLLQARDPVEAIRLRATRL
                     VVVRRGRVVARTLPVTAALELAGRPARVDWTLPR"
     gene            2156..2773
                     /locus_tag="VFB53_10230"
     CDS             2156..2773
                     /locus_tag="VFB53_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZN87145.1"
                     /translation="MKLIGSPTSPYVRKVRIFMAEKRLDYQFEVEDVWASDKILQSNP
                     LGKVPCLVMEGGEAVFDSRVIVEYVDTLSPVGKLIPDRGRERAEVRTWEALGDGVLDA
                     AVLARLENTWAGRNEGQRSAAWVERQTKKIHASLAAMSKGLGDKPYCSGIHFALSDIA
                     VCVALGYLDFRFAEIDWRGAHPNLARLYEKVSQRPSFVDTAPPRG"
     gene            complement(2855..3493)
                     /locus_tag="VFB53_10235"
     CDS             complement(2855..3493)
                     /locus_tag="VFB53_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN87146.1"
                     /translation="MTSVALLHRESELGHRLAETVAAAPGLTVLGSAGTMPALREVLA
                     QEVPDLLLVDLMLPGAQVQSVLQELRHEVPDGRPRLLVLAVSADDPRVMAALRHGADS
                     YFAHAHSLLSLPEAIEQLVRGESMITPQIARQLKSHFEAGGGAALPEADCRLLQWMAE
                     GFLVSEVARALRVNAHAVGVRIRSLYRRLQADVRSQQDVREDAGLQGGPLFT"
     gene            complement(3659..4792)
                     /locus_tag="VFB53_10240"
     CDS             complement(3659..4792)
                     /locus_tag="VFB53_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN87147.1"
                     /translation="MKAFSLARTVAIFLKEFQQMLRDRLTFAMAIGVPILQLVLFGYA
                     INTDPKGLPTAVVVHDTGPLARSVVAAMQNTGYFRVTHASASEEDAEALLAAGDVQFV
                     VAIPPDFGRRLVRGERPALLVAVDATDPSASGNAIAALGQVAPQALRADLVGPLAALQ
                     PGEAPYEVRIHRRYNPEGLSRYNIVPGLIGTILTMTMVMLTGLAMTRERERGTMENLL
                     ATPVRPVEVMLGKILPYVVIGYVQLGVILLAARALFEVPMAGSFLLLMAMIGVFMLAN
                     LAVGFTFSTLAQNQLQAMQMTFFFFLPSMLLSGFMFPFRGMPGWAQALGEALPLTHFL
                     RIVRGIMLKGNGAAQLLPEVWPMLLFLAAAGALALARYRQTVD"
     gene            complement(4789..>5313)
                     /locus_tag="VFB53_10245"
     CDS             complement(4789..>5313)
                     /locus_tag="VFB53_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN87148.1"
                     /translation="WKQRLALAACLIHEPRLLLLDEPTAGVDPKARRDFWDEIHRLAA
                     QGITVLVSTHYMDEAERCHELVYIAYGRTLARGTERDIVAQAGLVVWAVDGDDVAALV
                     EPLKAAPGVLSVAAFGSALHVAGRDGEALARAIAPYRERPGLRWRRADANLEDVFISL
                     IAQAQDNFAQPAAA"
BASE COUNT          767 a         1903 c         1946 g          697 t
ORIGIN      
        1 caccaagtga gaccgcttca gagccggtga tcgattcgcc tttgaacggt cggctcggct
       61 gcgggtgagc cgacgccgcg gcacagcttc gcagggtcgg ctcaggcttc gaccgcgtcg
      121 gcggtcgcgc ccgcggccgc ctggcccggg cggcgcgcgg gcgagcgccg ctccgtcatg
      181 gcgagcagca cgacggcgag caccagcagc gcgacgaaca gcccgcccgc gatgcccagc
      241 acgttctcca tcggcatgac gcggttccac ggtcgcgcga tgcccaggaa ggcggcgaag
      301 aaccacagcg tggtggacag cgccgccggc acggcgaccc cgaccacgtc gtcgcccgcc
      361 gtcatgcaca gcgggcgctt ggtctccagg cggggaaagg tgagccggtg cagcacgacg
      421 gcgttcaccg cgagcagggc caccagcacg atcttggcca tcagcttggg attgcccgtg
      481 agatagctcg gatcgcgcgc gatgccggcc gcgacgaggc cgccgcccgt gaccacgagc
      541 gcgagcagcg accaggccac gacgtcgcgc accatcggct cgggcggcag cagccgcccg
      601 ccgccacgcc gcatcttgcc gagcacgtgc aggtcgctca ccaggatcgc gcccagcgcg
      661 gcgcaggtgg cgaggaggtg gaggtacacc agcaggagat gggcggcctg gaacagctcg
      721 gcaggcatgc agccacctta ccccacccgc aatgggcccg gcccccgtgc tgactttggc
      781 cggcgccgga tcgcttagag tggccgcatg ctcgacctgc tgtttcgcga cgtcgccctg
      841 cccgacgggc ggcgcgacct ggacgtgcac gtgcgcgacg gccgcatcga ggccatcggc
      901 gcgcgcctgc atgcgcccga gggcgcggac gtcgtggccg ggggcgggca gctgctcgcg
      961 ccgtcgttcg tcgacgcgca cttccacctg gacgcgacgc tgtcgtacgg gctgccgcgc
     1021 gtcaacgcga gcggcacgct gctcgagggc atcgccctgt ggggcgagct gaagccggcg
     1081 ctcacccagg aggcgctcgt gcagcgtgcg ctcgcgtact gcgactgggc ggtcgccaag
     1141 gggctgctcg cgatccgctc gcacgtggac gtgtgcgacg accggctgct cgccgtggag
     1201 gccctgctcg acgtgaagcg gcgcgtcgcg ccctacctcg acctgcagct ggtcgccttc
     1261 ccgcaggacg gcgtgctgcg ctcgcccggc gcgctcgcca acctgaagcg tgcactcgcg
     1321 atgggcgtcg acgtggtggg cggcatcccg cacttcgagc gcacgatggc cgagggcgcc
     1381 gaatcggtgc gcctgttgtg cgagctggct gccgagcagg gccggcgcgt ggacctgcat
     1441 tgcgacgaaa gcgacgaccc actctcgcgc cacatcgaga cgctcgcctt ccacacgcag
     1501 cggctcggcc tgcagggccg cgtcaccggc tcgcacctga cgtccatgca ttccatggac
     1561 aactattacg tgtccaagct gcttccgctg atggccgagg ccggcgtgca cgcggtggcg
     1621 aacccgctcg tcaacatcac gctgcagggc cgccacgaca cgtaccccag gcggcgcggc
     1681 atgacgcgcg tgcccgagct gctggcggcc ggcgtcaacg tcgccttcgg ccacgactgc
     1741 gtcatggatc cgtggtacgg cctcggctcg gccgacatgc tggaggtggc gcacatgggc
     1801 ctgcacgtcg cgcagatgac gtcgcaggcg cagatgaagg cgtgcttcga cgcggtgacg
     1861 gtgaacgcgg cccgggtgct ggggctcgac gaccacggcg tcgccgtggg ccgctcggcc
     1921 gacctggtgc tgttgcaggc gcgcgacccg gtggaagcga tccgcctgcg cgccacgcgg
     1981 ctcgtggtgg tgcggcgcgg ccgggtggtg gcccggacct tgcctgtcac cgcggcactg
     2041 gaactcgcgg gccggcccgc ccgggtggac tggaccctcc cgcgatgacc ccacctcggc
     2101 gcgacggtct caccgctaga atgaatttga cccggacgcc ctccaaccac aatccatgaa
     2161 actcatcggc tcgccgacca gcccctacgt gcgcaaggtt cgcatcttca tggccgagaa
     2221 gcggctggac taccagttcg aggtggagga cgtctgggcg agcgacaaga tcctgcagtc
     2281 caacccgctc ggcaaggtgc cctgcctggt gatggagggc ggcgaggcgg tgttcgattc
     2341 gcgcgtgatc gtggagtacg tcgacacgct gtcgccggtg ggcaagctca tccccgaccg
     2401 cgggcgcgag cgcgccgagg tgcgcacctg ggaggccctg ggagacggcg tgctggacgc
     2461 ggccgtgctc gcgcgcctgg agaacacctg ggccggccgc aacgaggggc agcgcagcgc
     2521 ggcgtgggtg gagcgccaga cgaagaagat ccacgcgtcg ctcgccgcga tgagcaaggg
     2581 gctgggcgac aagccgtact gcagcggcat ccatttcgcc ctgtccgaca tcgcggtgtg
     2641 cgtggcgctc ggctacctcg acttccgctt cgccgagatc gactggcgcg gcgcgcatcc
     2701 caacctggcg cggctgtacg agaaggtgtc gcagcggccg agcttcgtcg acaccgcccc
     2761 gccccgggga tgagggcgcg gcctcccggg cggggggcgc cgacgctgcc tgcagcgcac
     2821 ccgggtgact gcgcgcccgg cgccgcgcag gcgcctacgt gaacagcggc ccgccctgca
     2881 ggccggcgtc ttcccgcacg tcctgctggc tgcgcacgtc ggcctgcagc cgccggtaga
     2941 gcgagcggat gcgaaccccc accgcgtgcg cattcacgcg cagcgcgcgc gccacctcgc
     3001 tcacgaggaa gccctcggcc atccactgca gcaggcggca gtccgcctcg ggcaaggccg
     3061 cgcccccgcc ggcctcgaag tggctcttga gctggcgcgc gatctgcggg gtgatcatgg
     3121 attcgccgcg caccagctgc tcgatggcct cgggcaggga aagcagcgag tgcgcgtgcg
     3181 cgaaatagct gtccgcgcca tggcgcagcg ccgccatgac gcgcgggtcg tcggcggaca
     3241 ccgcgagcac cagcaggcgc ggccggccgt ccggcacctc gtggcgcagt tcctgcagca
     3301 cggactgcac ctgcgcgccg ggcagcatca ggtcgaccag cagcaggtcg ggcacttcct
     3361 gcgccagcac ctcgcgcagc gcgggcatgg tgccggcgct gcccagcacc gtcaggcccg
     3421 gcgcggccgc gaccgtctcg gccaggcggt ggccgagctc ggattcgcga tggagcaggg
     3481 cgacggaagt catgggcgag tgcgcggcag gtcggttagc ggcgactatt cttccagccg
     3541 cctcgcgcgc ccacatcccc gcgtctgggg tgtgcagcgc cccccttgac ggcgccgggc
     3601 ctccccacct cgggggcggc gccgccgccg ggcggcgcgt gcaccgggca cgcgcgcctc
     3661 aatcgacggt ctggcggtag cgcgcgagcg cgagcgcgcc ggcggcggcg aggaagagga
     3721 gcatcggcca cacctccggc agcagctgcg ccgcgccgtt gcccttgagc atgatcccgc
     3781 gcacgatgcg caggaagtgg gtgagcggca gcgcctcgcc gagcgcctgg gcccaccccg
     3841 gcatgccgcg gaacgggaac atgaagcccg agagcagcat ggagggcagg aaaaagaaga
     3901 acgtcatctg catcgcctgc agctggttct gtgccagcgt ggagaaggtg aagcccaccg
     3961 ccaggttggc cagcatgaac acgccgatca tcgccatcag cagcaggaag ctgcccgcca
     4021 tcggcacctc gaacagcgcg cgcgccgcga gcaggatgac gccgagctgc acgtagccga
     4081 tcaccacgta cggcaggatc ttgccgagca tgacctccac cgggcgcacc ggcgtggcga
     4141 gcaggttctc catcgtgccg cgctcgcgct cgcgcgtcat cgcgaggccg gtgagcatga
     4201 ccatcgtcat cgtgaggatg gtgccgatca ggcccggcac gatgttgtag cgcgacaggc
     4261 cctcggggtt gtagcggcgg tggatgcgca cctcgtacgg cgcctcgccc ggctgcagcg
     4321 cggcgagcgg cccgaccagg tcggcgcgca gcgcctgcgg cgccacctgg ccgagcgcgg
     4381 cgatcgcgtt gcccgaggcc gaggggtccg tcgcgtccac cgcgacgagc agcgccggcc
     4441 gctcgccgcg cacgaggcgc cggccgaagt cgggcgggat ggcgacgacg aactgcacgt
     4501 cgcccgcggc cagcagcgcc tcggcgtctt cctcgctcgc gctcgcgtgc gtcacgcgga
     4561 agtagccggt gttctgcatc gccgcgacca cgctgcgcgc gagcgggccg gtgtcgtgca
     4621 ccaccaccgc ggtgggcagg cccttggggt cggtgttgat cgcgtagccg aagagcacga
     4681 gctgcaggat cggcacgccg atcgccatcg cgaacgtgag ccggtcgcgc agcatctgct
     4741 ggaactcctt gaggaagatc gcgacggtgc gcgcgagcga gaacgccttc atgcggccgc
     4801 cggttgcgcg aagttgtcct gcgcctgcgc gatgaggctg atgaagacgt cctccaggtt
     4861 ggcgtcggcg cgccgccagc gcaggcccgg gcgctcgcgg tacggcgcga tcgcgcgcgc
     4921 gagcgcctcg ccgtcgcggc ccgcgacgtg cagcgcgctg ccgaaggcgg ccacgctcag
     4981 cacgccgggc gcggccttca gcggctcgac gagcgcggcg acgtcgtcgc cgtccaccgc
     5041 ccacaccacg agccccgcct gcgcgacgat gtcccgctcg gtgccgcgcg cgagcgtccg
     5101 gccgtaggcg atgtagacca gctcgtggca gcgctcggcc tcgtccatgt agtgcgtgga
     5161 gacgagcacc gtgatgcctt gcgcggcgag gcggtggatc tcgtcccaga agtcgcgccg
     5221 cgccttgggg tcgacgccgg cggtgggctc gtcgagcagc agcaggcgcg gctcgtggat
     5281 caggcaggcg gcgagcgcga ggcgctgctt cca
//
LOCUS       DASRIE010000239        42058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1550256, whole genome shotgun sequence.
ACCESSION   DASRIE010000239 DASRIE010000000
VERSION     DASRIE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 42058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550256"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..724
                     /locus_tag="VFB53_10250"
     CDS             131..724
                     /locus_tag="VFB53_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZN87149.1"
                     /translation="MEMTGEERIPASQAGTWAALNDPEILKACVPGCESIERLAENEY
                     QVLMVARIGPVSAKFKGKLALSDLKPPHSYAIAFEGQGGAAGFGKGAAQVQLAPDGDG
                     TRLSYQVHANVGGKLAQIGSRLVDGAAKKIAQEFFAAFNEKVGARHAPAGDAHAERHP
                     EPVARDPALPTVTDASLVFLAAGSLVVFVVALFALLG"
     gene            868..1476
                     /locus_tag="VFB53_10255"
     CDS             868..1476
                     /locus_tag="VFB53_10255"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN87150.1"
                     /translation="MATQRDNPAERTPAVILFAEMRNFTSMSEMLDAARVLALADQFF
                     DSAAAAVQANGGEVIGVHNDSLLAAFRRGKPAEQGRGAVRAAQRMATDFDPVAQAWQR
                     DYGLRVAIALGMHLGDAVFGEAGPAAQRNDVVFGDCVSIAERLVHRARAGELVLSHAV
                     MAVAPVAELELDAQSLPPLELHNRPPIRIYGVLRDDRLDFTS"
     gene            complement(1523..2062)
                     /locus_tag="VFB53_10260"
     CDS             complement(1523..2062)
                     /locus_tag="VFB53_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN87151.1"
                     /translation="MRGHGLILSTLLVACGVGLACAQDAKRTPDVIYVPTPQEVVEDM
                     LRLADVRKGDVLYDLGSGDGRIAVTAAKKYGIRAVGIDIDPERIREANDNARKQGVAK
                     LVQFRREDLFAADFREATVVTLYLLPDLNLKLLPLLQAQLKPGTRVVSHQFDMGDWRP
                     AKVLESNGRTVYFWVMPRK"
     gene            complement(2127..2399)
                     /locus_tag="VFB53_10265"
     CDS             complement(2127..2399)
                     /locus_tag="VFB53_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87152.1"
                     /translation="MAPSLDPHTGHEHPLVAWTSILAILAAILLISWARIEVHGTGDL
                     EAALTAGDADAASAWRNLSPERDRTPHEKAVDKALAAGRWAAGFSP"
     gene            complement(2463..3818)
                     /gene="pmbA"
                     /locus_tag="VFB53_10270"
     CDS             complement(2463..3818)
                     /gene="pmbA"
                     /locus_tag="VFB53_10270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZN87153.1"
                     /translation="MKNGGRFGYAQEQLQQLAADVLAHARRLGASACECEVSEGHGLS
                     VTVRKGAVDTIEHNRDKGIGVSVYLGERPAARRGHASTSDFSAAALEQTVAAALAIAR
                     QTAADDCAGPPESELLARELPDLDLFHPWMPATEEAIGIAQRCEAAAFALSPKIRNSE
                     GASVSAQHSQFVFANSLGFMGGFPASRHSIYLSVIAEDRRGMQRDDWYSAARVPAQLA
                     RPEALGRYAGERALARLGARRIGTRQAPVLFEAPQAAMLLGSFVSAASGGNLYRKASF
                     LLDGLGKQVFAPGITLEERPHEPRALASSPFDEEGVATRARTVVRGGVLQGYFLGCYS
                     ARKLGMQSTGSAGGNHNLVLAPNGPDFRGMLKRLHRGLLVTELLGHGVNLVTGDYSRG
                     AAGYWVENGEIAHPVEEVTIAGNLRDMFRGIEAVGSDTVVRGSRRSGSILLREMTIAG
                     E"
     gene            3817..4323
                     /gene="yjgA"
                     /locus_tag="VFB53_10275"
     CDS             3817..4323
                     /gene="yjgA"
                     /locus_tag="VFB53_10275"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZN87154.1"
                     /translation="MARYHSSAMQDELTSKTKKKQEMHALQKLGAALVELSAAHFGSM
                     ALPEALQLAVQEARRIRSHEAKRRQLQLIGKLMRGLDAEPIRAQLAAVSGGSAQERAR
                     HQRLEHWRARLMADDTALTAFASEHREADLQNLRSLLRNARKEQKAGKPPRAYRELFR
                     LIRKAAGE"
     gene            4310..4873
                     /gene="mog"
                     /locus_tag="VFB53_10280"
     CDS             4310..4873
                     /gene="mog"
                     /locus_tag="VFB53_10280"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HZN87155.1"
                     /translation="MPVSDALIVGLLSASDRASHGVYRDEGLPALKDWLSKAVLAPAL
                     RYEERLVPDEQAVIEAALVELVDVKGCHLVLTTGGTGPAPRDVTPEATLAVADRTMPG
                     FGEQMRAVSLKYVPTAILSRQVAVVRKQALIINLPGQPKAIKETLDGVFAAVPYCIDL
                     IGGPYIETDESLVKAFRPKSAQRPKPA"
     gene            4870..5544
                     /locus_tag="VFB53_10285"
     CDS             4870..5544
                     /locus_tag="VFB53_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN87156.1"
                     /translation="MTQLPEAVEVETAPAPDAAVIWLHGLGADGHDFEPVVPELRLPA
                     RLRLRFVFPHAPVRPVTINMGMPMRAWYDILQMGGGREDAAGIRASQGLLEALVARER
                     DRGIAAARIVLAGFSQGGAIALHTALRHAERLAGVLALSTYLPLAATAAAERSAASRD
                     LPVFMAHGSQDPMIDIARAREARTLLEGLGAAVQWREYAMPHSVCAEEIGDIAAWLLS
                     RLAPSP"
     gene            complement(5519..7024)
                     /locus_tag="VFB53_10290"
     CDS             complement(5519..7024)
                     /locus_tag="VFB53_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87157.1"
                     /translation="MIKLFGRSGADHPMADAKEARRVLDALPAQDSCKALEELAEWHE
                     SVSQSEGFRPEQRIERLLAIDDAAQGRLARLTRDYLAQSRPARFQENRMWLAAHGYWR
                     EAGLAFARAVDLFVQGAKGADAARAALPQLLVRCLRALAQQIKWMHLRYGPYDAAAWA
                     TFNKVYAFAESRKLAQTASAANPGMPGASTPQLEFLRGALFAASSPEGLLPAEIELAE
                     RLIAGLAGRFALAAAASAELPVWTDLAKAMAPQRALRPPPAAPGLRWLGAGSALAEAK
                     ALAERIRATDAVPAGLNLDPGAMPEDVLAVLEHLVLYWSPQPPERKFQRHSVQSRLSV
                     SNGLKGVIDTLGGPGAAGAESWVIENVSAGGFGAIVPQVKGDWLRVGALVAMQPDGGS
                     NWIVGLVRRVNKTAAQQARVGIQTLSRVPAVSQFALSGARGSAEQGVLLKGGDPASNE
                     VRIALRPGIFAPGQNLEASRDGRQHIYMPTGIGARGEDYEIGRFREMVRDG"
     gene            7050..8222
                     /locus_tag="VFB53_10295"
     CDS             7050..8222
                     /locus_tag="VFB53_10295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN87158.1"
                     /translation="MRFLIVDPSGEFRSTLAGMLRARWPLAFTDEWDPRAHGSPRAAL
                     ARERYAAVLIAAQAEGGEEWVAEMRRDPAAPPLVMFKQDGSESLVRSVEGVLREQEAR
                     RAEVPGTPVFPPAAQAAAPKADSPVDRDERAIPGYRSLRKIGEGGMAQVYLAEREQDG
                     LQLVLKVLDPRLRSDQTFFKRFVQEYRLVAGLHNEYVAHIYDQGFAGEHPYIAMEFLS
                     GGTLATRIHEGVTSLAALRITSQIARALDAIHAHGIIHRDLKPQNILFRDNGRPVIVD
                     FGLAKNLGAKTTLTRHGQVIATPRYMSPEQCLGKAADHRSDLYSLGVIFYEMLSGLKL
                     YDAEGPAGLVYQHVHGPIPRLPGRLAGYQGIVDRLLAKTPEARFQSARELFAMIAV"
     gene            8219..9031
                     /locus_tag="VFB53_10300"
     CDS             8219..9031
                     /locus_tag="VFB53_10300"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZN87159.1"
                     /translation="MSAGAGERQYLALLEEVLARGAPKSDRTGTGTRSVFGRQLRFGL
                     AQDFPLLTTKKLHLKSILYELLWFMRGDTNVRWLQQHGVSIWDEWADADGELGPVYGY
                     QWRHWRTPEGREIDQLRQVMEQIKARPDSRRHLVTAWNPGDIERMALPPCHALFQFYV
                     AEGCLSCQMYQRSADLFLGVPFNIASYALLTLMVAQVAGLRPGEFVLTLGDAHLYLNH
                     LEQAREQLRREPRPFPRMRLNPAVRDLFEFRYEDFTLEGYDPHPAIKAPIAV"
     gene            complement(9108..9440)
                     /locus_tag="VFB53_10305"
     CDS             complement(9108..9440)
                     /locus_tag="VFB53_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87160.1"
                     /translation="MDMEQANTKFEVPILAQVLIGFMVSVLVMVGIAGSIYKLVSPDG
                     WIALAFGHSFSFGAALLAALLGAAAVVWTVPGFAPGQRARIADAVVYSFAAAGVLFLG
                     QLLVLGSF"
     gene            complement(9630..10988)
                     /locus_tag="VFB53_10310"
     CDS             complement(9630..10988)
                     /locus_tag="VFB53_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN87161.1"
                     /translation="MNQEAPSILLVDDDRDLLHLLSMRLTAAGYRVTAVEAADAALAS
                     LAVQRPQLVITDLRMQGMDGLGLFDAIHRQAPSLPVVILTAHGTIPDAVAATRRGVFS
                     FLTKPFEPKVLLETVADALRLSTAPSGDRAQWRSEIITRAATMEALLGQAQRVAASDA
                     SVCIFGASGTGKELLARAIHRASRRAGAPFIAVNCGAIPEGLLESELFGHKKGAFTGA
                     VQDRRGLFQACEGGTLFLDEVGDMPPALQVKLLRAIEDREVRPVGANDSVAVDVRIVS
                     ATHRNLEERIASGEFREDLYYRLNVVKLELPALERRREDVPLLAGHFLARFAERYDRP
                     RLGMSPEAMEVLVSAAWPGNVRQLLNVIERAVALTATDVIPASLVRQALDAGAGSLTP
                     LDEARRAFEREYLVRILKITGGNVTQAARLAGRNRTEFYRLLERHALEPAMFKIKVAA
                     ES"
     gene            complement(10993..11493)
                     /locus_tag="VFB53_10315"
     CDS             complement(10993..11493)
                     /locus_tag="VFB53_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87162.1"
                     /translation="MRPATVLCAALLSGCALLQPDAGEALQVRDIVAAAASGARAAPE
                     EQRRQLTHAQQMFAAVPDDGNRVRLAALLANLPAPMRDDARAVALLEPLAARRPETPL
                     VQLAALLSAGVAERQKLARDLRAAEKREEAAEERATTLRQQVEALKSIERGILEREER
                     RRTIKR"
     gene            complement(11490..12971)
                     /locus_tag="VFB53_10320"
     CDS             complement(11490..12971)
                     /locus_tag="VFB53_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN87163.1"
                     /translation="MAPQDTSPGAALRFPRSFLGLLVTGFLLVAAPLAGALVYSAWNT
                     QRLAELSRSAVYNAAQAARGSRALVNRIASIERLAQQVALLPDPGLVQDYERLHAGFR
                     QLSDELARLPLDGEQLTALRRTIGEEQALYELLSGASGSPPDARAVNEKVSDLAESAH
                     EVQAISYRVADGEVERLRAAAEVVQRRLLQLVLATIAVALAFALALPRIIARPIAELD
                     ASIRQLGGADFSRPIRVSGPQDLRDLGERLDWLRQRLTELEAQKNRFLRHLSHELKTP
                     LASLREGAELLNDQVAGPLAPPQRAVVGIMRDNSIKLQKLIEDLLDYQRALHAAASLE
                     VRTVRLDTLAREVADAHRLAALAKAQRVALDLAPVAVEADAEKLRSIIDNLLGNAIKF
                     TPAGGSISITTREAEGGVVVEVIDSGPGVPAVEREAVFESFFRGRAKAGGRVEGSGLG
                     LAIAREYVEAHGGRIALVAEGPGGHFRVTLPKRPAPLVLAEAA"
     gene            13013..13522
                     /locus_tag="VFB53_10325"
     CDS             13013..13522
                     /locus_tag="VFB53_10325"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZN87164.1"
                     /translation="MSQAVARPCATRLYLVAAVAANGVIGAHGKLPWHLPEDLRHFKA
                     LTLGHPVVMGRKTWESLGRALPGRENIVVTRAPGYAAPGASVASSLEAALALCAGEPT
                     VFVIGGGALYAEALALADGLVLTEIQRDYAGDARFPDYDRAAWRETQRKPQTGADGLR
                     FDFVLYERA"
     gene            complement(13536..14741)
                     /locus_tag="VFB53_10330"
     CDS             complement(13536..14741)
                     /locus_tag="VFB53_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZN87165.1"
                     /translation="MGDRPSSLVRNRQQSSLPQSQLFDTHAEVSLDFEIVRRAARQKY
                     TRPFLIIDSAIVREKLRRFRAAMPRVRPHYAVKANPDRRVLKALVQEGAGFEIASTAE
                     LDLLLSLGVNAAEVFFSNPMRSRDADAYAVAKGVRWFVVDSVDELRRMHAVDPSAHLY
                     LRIATPNIGSDWPLSGKFGAGAGDTREIIATAAKLGADLAGVTFHAGSQCRNPENWRV
                     GIEKARTLFDGMLKAGLKPRLLDIGGGFPVRHVKPIPSIEVIGEVANEALKAFPEDVQ
                     VIAEPGRYLVSDAGYFVCRVLGTATRAGKRWLHWDAGLFGGVIETTEGLKYKIRTDRS
                     GPDVAWNVAGPTCDSVDVVMRDEPLPSDLQEGDFIYLRNAGAYTTAYASEFNGFPLPE
                     VRVFESRTK"
     gene            complement(15036..15599)
                     /gene="dcd"
                     /locus_tag="VFB53_10335"
     CDS             complement(15036..15599)
                     /gene="dcd"
                     /locus_tag="VFB53_10335"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZN87166.1"
                     /translation="MTVKSDKWIRRMAAQGMIEPFEAGQVKEAGGQRVVSYGTSSYGY
                     DIRCSDEFKIFTNINSTIVDPKAFDAKSFVDFKGAVCIIPPNSFALARTVEYFRIPRS
                     VLTICLGKSTYARCGIIVNVTPLEPEWQGHVTLEFSNTTPLPAKIYAGEGCAQVIFLE
                     SDEVCETSYRDRGGKYQGQQGVTLPRI"
     gene            complement(15596..16681)
                     /gene="apbC"
                     /locus_tag="VFB53_10340"
     CDS             complement(15596..16681)
                     /gene="apbC"
                     /locus_tag="VFB53_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZN87167.1"
                     /translation="MPVSDTEVRAVLSQLIDPNTGKDYVSSRSVKAVKVDGAEVRVDI
                     ELGYPGKSQFEPIKREITAALKKAGAGGVAASVSSRVVSHSVQRGVKLVPGIKNIIAV
                     ASGKGGVGKSTTAVNLALALAAEGATVGILDADIYGPSQPTMLGIAGRPESKDGKGLE
                     PMMGHGLQAISIGFLIDVDTPMVWRGPMVTQALEQLLKDTRWRELDYLVVDLPPGTGD
                     IQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGIPILGIVENMSIHVCPKCG
                     HESHIFGSGGAARMCQDYGTELLGQLPLDESIREQADSGRPTVVADPDGKAAQIYRRI
                     ARRCAVRIAESQKDMSGKFPNIVVQNT"
     gene            16725..18755
                     /gene="metG"
                     /locus_tag="VFB53_10345"
     CDS             16725..18755
                     /gene="metG"
                     /locus_tag="VFB53_10345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZN87168.1"
                     /translation="MPRRRLFVTTALPYANGPFHIGHIMEYIQADIWVRFQRMQGHAV
                     HFVGADDAHGAPIMLAAEKAGAAPEAFVARIAKGRKQYLDGFHIAFDNWHSTHSPENT
                     QLSQDIYRRLKKAGLVYTKPVEQFYDPVKGMFLADRYIKGECPKCGTRDQYGDACENC
                     SAAYSATDLKNPYSTLSGAKPVLKTSEHHFFRLSDPKCRTFLKGWLEKPGRLQSQVVN
                     KAKEWLDGEGDKALGDWDISRDPPYFGIRVPDIAEEKYLYVWLDAPIGYLASFKNYCA
                     RTGIDFEKFLQDPGTEQVHFIGKDIIYFHTLFWPAMLEYAGKPYKVPDHVYVHGFITV
                     SGDKMSKSRGTGISPLRYLELGMNPEWLRYYIAAKLNANVEDIDFNPDDFVARVNSDL
                     VGKYVNIASRAASFIAKHFEGKLYDFGSDPAPSWAAAVAIDDEIAAAYEAREFGKVVR
                     EVMAVADRVNQRFDQAKPWELAKDPSRIGDLHRACSDALQAFLTLTVYLAPMLPVTAV
                     RVARELFGLERDLLFDDRKLRATAIRPFKHLATRVEERQLDQLFDIAPAPAPAAKEKS
                     VSDPITIDDFNKLDLRVARIAKAEHVEGADKLLKLTLDLGGSTRTVFAGIKSAYDPAG
                     LEGKLAVVVANLAPRTMKFGVSEGMVLAASGAEPGIFLISPDAGATPGMRVK"
     gene            18793..20028
                     /locus_tag="VFB53_10350"
     CDS             18793..20028
                     /locus_tag="VFB53_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN87169.1"
                     /translation="MIEQPSRTLVCSIVFLDIVEYSTKPVAEQLVLKQAFNRLLSSSL
                     EYVPARDRVVLDTGDGAGITFLGDPEDALFVAMSLRAAARTEATGAIAIRIGINLGPV
                     RLVKDINGQMNIIGDGINVAQRVMTFAQSGQMLVSRSFYEVVSCLSLDYASLFSYVGA
                     RTDKHVREHEVYSVGIGTDAARRVLETGSRRVAPRGSAPRLIGELTDVRPFGVHRMAL
                     LLTPLLFALLVGGGFAVRAQVRPAEAQAPAAKAQPPKPALTPVAAKAAAAPEKPVAVA
                     LAQPAAKAAALQPKAEPQPAAPKPVKTLTPAQKEARREALAARAARAQAAKTEAAQPA
                     QAAASVATASGTVQLAILPWGEVFVDGKRRGVSPPLRSIEVPPGTHTIEVRNTSFPSH
                     AQRVEVRVGEPVRIRHHFR"
     gene            20078..20509
                     /locus_tag="VFB53_10355"
     CDS             20078..20509
                     /locus_tag="VFB53_10355"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HZN87170.1"
                     /translation="MGRRQFGNQASTRIAILAALAAVALLAGCQSEPIRGLQADFKGL
                     FGKSKGEPALAAGLRQYEDGNYAEAAKQLQSAIGQGLTSNERVQAHKHLAFIHCASDR
                     TAACREEFRRALAIDPGLELSAAEAGHPSWGPVFRALKTGR"
     gene            20528..21757
                     /locus_tag="VFB53_10360"
     CDS             20528..21757
                     /locus_tag="VFB53_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN87171.1"
                     /translation="MENTKLGRFEIVAEIGRGAMGIVYKAQDPMLERTVAIKTINMGM
                     DRDGAEMYEKRFYQEARAAGGLNHPNIVTVYDIGKTETACYMAMEYIEGAELRTLLLP
                     GKPLPVARALSIAAQVAEGLAYAHERGVVHRDIKPANIMVPESGAVKITDFGIARMRT
                     SNVQTQTGMMMGSPKYMSPEQVIGKRADHRCDIFSLGVILYEMLTGASPFTGESVNAV
                     MYQIVNFVPPAPSAINPAAPAALDGIVGRMLAKALEERYQSAAEIARGLRDCEHQMRD
                     AGGGAAPSPDTASTVPVSALAPGPAPAVVDTQVKAAVLAQTVERSRRAEEETPSPDAA
                     PARGLSRTFDSLEATQRLVALSDAAVPSDPAASTLPLTMQTTAMVMAGGWKQRDTVVV
                     AAGAVAGLIVAALIVWA"
     gene            21748..23754
                     /gene="hyfB"
                     /locus_tag="VFB53_10365"
     CDS             21748..23754
                     /gene="hyfB"
                     /locus_tag="VFB53_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HZN87172.1"
                     /translation="MGIEESGALSASLAAAAAWIAIGAAGLLRPASLVLASRVLFPAG
                     AAVGCALAAWALQGVFATPQALVLPLGLPDLPFHARIDALSAFFLLLLGAVAAGVSLF
                     ASGYLRPGQGTSPGLQCLYYHVFLASMALVLIADDAYFFMVAWETMALASYFLVTSEH
                     RHEEIRRAGYLYLLMAHIGAIAILLCFGALQAGTGDYTFHNMRELQPGAEVATAAFLL
                     ALVGFGVKAGIVPLHVWLPEAHPAAPSPVSALMSAVMLKTAVYGMLRVSLDLIAFPLW
                     WWGVVALVLGLVSALGGIVFAAVQTDMKRLLAYSSIENIGLVFAGFGLTLIFRSFGMG
                     TLAALALTATLYHCLNHAFFKSLLFLCTGSVLHATSERSLGKLGGLIHRMPWVAWCAL
                     VGSVAIAGLPPLNGFVSEWLLLQAFLLTPGLPQAYLNMLVPVAAAVVALTAALSGYVM
                     VKFYGVVFLGQPRDARLAEAHDAGAWERTGLAWLAAMCIVLGLFPAAVIGLLDPITRA
                     LTGHGMASLAQGWLFLAPLQPERASYSALLFFAVTGLLVALTWLAVRAFYHGRVRRAP
                     PWDCGFPLQNARMQDSAEGFGQPVRHVFEPFFRVEQSAPAPADARPSYTEVHDDRLWH
                     WLYLPLARGADRLTGLVTFLQRGRISVYLSYSFATLLVLLLFVG"
     gene            23761..24711
                     /locus_tag="VFB53_10370"
     CDS             23761..24711
                     /locus_tag="VFB53_10370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HZN87173.1"
                     /translation="MASLQGLVAQFAVLALTVLLAPMLTGWVNQCRAWLLNRSGPGLL
                     QPYRVLAKLFSKEVVLAHNASPLFRGAPYVIFGCLALASAIVPKIGTDLPLNAAADAI
                     ALVGLLALARLFTALAGMDIGTAFGTLGARREMLVGFLAEPALLMVLFTASLISGSTS
                     LATIVEVLAHREFVIYPSLAFAGVAFTMVSLAENARIPVDNPATHLELTMIHEAMVLE
                     YSGRHLALIEWAASLKLLVYSCIGLALFFPFGIAERGDWRGLLLALPVLALKLAAGGF
                     LLALIETLCAKMRIFRAPEFLATAFLLAVLAMLLHVLLGG"
     gene            24716..25375
                     /locus_tag="VFB53_10375"
     CDS             24716..25375
                     /locus_tag="VFB53_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HZN87174.1"
                     /translation="MQHYGPQLVNLLAAVLLLLSFAMLAQRRILSLINLYAAQGCVLF
                     LSTALVAWLTGQNHLYGSAALTLALKVIALPWILHRLIRRLNVKWDVETLINIPATML
                     VGIVLVILAFNLALPISQLAGTVTRSTFGIALASVLLSFLMMITRSKAVPQVIGFLAM
                     ENGLFFAATGAVYGMPMVIEFGVALDVLVGALILGIFVFRIRERFDSVDTRHLEKLKE
                     R"
     gene            25375..26829
                     /locus_tag="VFB53_10380"
     CDS             25375..26829
                     /locus_tag="VFB53_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HZN87175.1"
                     /translation="MAPEIALLLASPLAGGLALALFGHRDRAAELNIAFSLLTFVAAA
                     ALVARVGAEGPLLAARAQFFVDSLNVFLVALTAFVSLTTALFSAPYMRVERDKGRVSP
                     GGLRLYHSMYQLFSLAMLVVLTTNNLGVMWVAMEAATLTTVLLVALYRTHESLEAAWK
                     YFILCGVGIAQALLGTILVYFAAEKILGAGVGSLLWTQLDAVKGQLEPTVMSIAFVFL
                     LVGYGTKVGLVPLHNWLPDAHAEGPTPISAVLSGLLLNVALYALIRFKVITDGALERP
                     FASALLMGFGLVSVVVAAFFLSRQRDVKRMFAYSSIEHMGLITFAFGMGGPVASFAGL
                     LHMTVHSLTKSAIFFAVGHAAQQTGTQVMAEIRGLIRVNPTVGWGLMLGTAAILGMPP
                     FGVFASEFLILTTAIALQPWATPILLVSLGIAFAAIFGKVQPMVFGDTSAGRLPHPPA
                     LVPVFTHLALVLSLGLYMPPYLADWYRQAARLIG"
     gene            26834..28393
                     /locus_tag="VFB53_10385"
     CDS             26834..28393
                     /locus_tag="VFB53_10385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZN87176.1"
                     /translation="MAPQLPGISLTALPGAVPAWCAAVDTRQWRSGCEAAVRAGARLA
                     ALWASDERDAGGGYAVRALYVLFEGLACLELALPERDPRYPDLSDLYPCAGRMQRAAF
                     DLVGVRAATPEGAAPDTRGWLRHGAWRETAFPLRRDVSLAAALEPADPRYAFVQVAGD
                     GVHEIPVGPVHAGTIEPGHFRFSVVGEQVLRLEERLGYKHKGIEKRFASLTLEEGARL
                     AARVSGDSTVAYGWAYAMALEGLTGTRPGGRALLLRALLLERERIANHLGDLGALGND
                     GGLAFGLAQFSRLKEDLLRANARLFGHRYLMDRVVPGGVACDLPGAAAVEILEAGERL
                     AEELRSLRAIYYDHSGLQDRCITCGRLAPRTAALLGVVGLAARASGHAMDARAHPGFA
                     PYGELQPAVASHRNGDVAARIVVRFEEAFESLRLQRLILERLPDGPLRAPLGAARDGA
                     FGIGWVEGWRGEVIVALQSGAHGRVRRCHPHDPSWQNWPALEYAVIGNIVPDFPLINK
                     SFNLSYAGVDL"
     gene            28396..28920
                     /locus_tag="VFB53_10390"
     CDS             28396..28920
                     /locus_tag="VFB53_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HZN87177.1"
                     /translation="MHKILFKIAQVGLRSEPAPAADDTLRQGAPRLNEEVLRLFGRAL
                     AIRHVDAGSCNGCELEINALNNPYYNLEGRGIKFVASPRHADLLLVTGPVSRNMEEAL
                     LRTYAAVPEPKLVVAVGDCGCDGGVFGENYASRGRVANVIAVDVAVPGCPPPPAAILQ
                     ALVTAVTALAAAKR"
     gene            complement(28922..29596)
                     /locus_tag="VFB53_10395"
     CDS             complement(28922..29596)
                     /locus_tag="VFB53_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87178.1"
                     /translation="MRRSLHQRLDLAGAAIAHPTAQAEPFGLPLQGVAKADTLDAAAD
                     QQADCPHLSETRRSALAMLWAMNVHGKLRRKIVTPPRVRGRSLQKGDLTMRAALLVIA
                     GLALAGFLAVAFVLPQLAGSDAKEAAQALIAGAEAARQQVAAAAEKSGQLAGAGASAK
                     LAARTDPKHGELKWIVEASGAIRGWNEKNAIEVSITPALQGGKVTWNCRGYPITVMPA
                     SCGGKS"
     gene            29504..29728
                     /locus_tag="VFB53_10400"
     CDS             29504..29728
                     /locus_tag="VFB53_10400"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZN87179.1"
                     /translation="MQREAERLSLRGWVRNRRSGEVEALVQGPAQAVAALQDWARRGP
                     PAARVERIECGAPCPEHARDYARFERWPSA"
     gene            complement(29695..30462)
                     /locus_tag="VFB53_10405"
     CDS             complement(29695..30462)
                     /locus_tag="VFB53_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN87180.1"
                     /translation="MPLTHRLVDALKKCLRARGMTYAALARELRLSEASVKRLFSHGT
                     FTLARIEDILRVLELDLVELARMARGADDQAAELSLEQETLLASDERLLSVFWLLLNR
                     WAFDEIVEEFAISRTELTLALAKFERARLIDWDAGERVRLRVARDFVWRAGGPVKKAY
                     GHRVTTEFLRSRFSGPLELLRFEARDLSPASAAMLRRRLERVAAEFNELAEVDSTLPA
                     RRREGVGLLIACRPWAFSVVNALKKRAQALGQRSNRA"
     gene            30576..31538
                     /locus_tag="VFB53_10410"
     CDS             30576..31538
                     /locus_tag="VFB53_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN87181.1"
                     /translation="MLATQSLPLPALPGPRVHSALDLCKAVLAGGGLPDPLPTSGLDR
                     TLRHDAARGLLEVQAGATWHSLAAHTGGVVLPGTVGDSVAANSAGPDGRPVVAHLRAL
                     TLVTADGELRRASRDRAPELFRLAVGGFGAFGPFYSLTLDLATLSQAVAEAQAPVCLG
                     LAPDEAPGALHGLDLLVPPSAGGQFAAQARAALEGRRCALTRLEVRRVLREHETLLCW
                     ARRDYAAVHIEFRTRQTIGACVNAAELRRQLIALAIDAGGACAPQHLPHASRAQAAAC
                     YPMLGAFLAEKRRYDPAERVVGTWYRAVRGLWRRETCEVRWSRA"
     gene            complement(31535..34972)
                     /gene="dnaE"
                     /locus_tag="VFB53_10415"
     CDS             complement(31535..34972)
                     /gene="dnaE"
                     /locus_tag="VFB53_10415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZN87182.1"
                     /translation="MAAPRFVHLRLHSEYSVTDGIVRLDAAVQRAAGDGMPALALTDS
                     ANLFGMVKFYRAARAAGVKPVIGADCWVQGEAAGDKPSRLLLLCASRAGYLRLCELLS
                     RAWLKNQHRARAEIARSWLREGGSEGLIALSGAAGGDVGQALAAGGAAAAEKAAREWA
                     ALFPGRYYLEVQRAGFAHGEVLLARSLELAARLGLPVVATHPVQFLAADDFKAHEARV
                     CIAQGYVLGDQRRPKLFTPEQYFKSQAEMAQLFADVPQALDNALEIARRCSLEIELGR
                     SRLPAFPTPPGATVDQVLRQQAEAGLAARLAKLFPADGARAAAEPRYRQRLDFEIRTI
                     IQMGFAGYFLIVADFISWARHNGVPVGPGRGSGAGSLVAYSLGITDLDPITYDLLFER
                     FLNPERVSMPDFDIDFCQDGRDKVIDYVRRKYGEDCVSQIVTFGTMAARAVVRDVGRV
                     LDLGYNFCDQIAKLIPFQPGRLITLQDAREMEPLLAQREKNEDEVKELLALGEKLEGL
                     TRNVGMHAGGVLIAPGRLTDFCPLYAAEGTSNVISQLDKDDVEAVGLVKFDFLGLTTL
                     TILDWAERYVRALGAAEFSLERVPLDDAATYQLLSAGNTTAVFQLESRGMRDLIKRAR
                     PDRFEDVIALVALYRPGPMDLIPDFIARKHGSQRVEYLDKRLEPILSPTYGIMVYQEQ
                     VMQIGQVIGGLTLGGADLLRRAMGKKKPEEMAQQRDVFVAGAEKNGLSRGRATQLFDL
                     MEKFAGYGFNRSHAAAYALLAYQTAYMKAHHGAAFMAANLSLVMDDTDKVRQFHEDAL
                     ANGLTVLPPDVNASDYRFRPVDAKTIRYGLGAVRGTGEAAVGAILQARRSGPYTDLFD
                     LCRRVDKRIVNRRAVEALVRAGAFDTLQKNRASLLASVGRALEAAEQAERQAAQVSLF
                     GDAESARGAAGPLVETPAWDLRRLLLEEKAALGFALSGHLFSVYAREIAGFPRTPLAR
                     LSAGEQRVWLAGVVNAARSQMTRRGRMMAVLLDDATAQVEVTVFSELFERHRDKLKED
                     SLLVVQGKVQRDEFAGGMRVIAEELHDLQGLRARYAARLRLEMNGQADARQLMELLAP
                     YRADGACPVFVHYRTGAAACDVALGDAWRVRPEAQLIERLGAWLTPENVQVVYAGAA"
     gene            35002..35865
                     /locus_tag="VFB53_10420"
     CDS             35002..35865
                     /locus_tag="VFB53_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN87183.1"
                     /translation="MRAYALVALFAGATCIALSPIWVRVADVGPTASAFWRVALAAPL
                     LWGALAFTARAGAARPHWPLLAAAGFAFAGDLAFWHWSIQTTSVANATLLANLSSIFV
                     TLAAWLLWRQRPSAQFVVGLALALVGVALLVRTSLGVSGTALLGDGLGVITAVFYAWY
                     LLTVKRLRDLGAATLRLMAVTTTLTAVILFPVALASGEALLPAGARGWLVLLGLAWIT
                     HAGGQGLITYSLAHLPAAFSSVGLLLQPVMAAAFAWTLLGEPLAAPQVAGGAVVLAAI
                     YLARRGSKGSE"
     gene            35905..36369
                     /locus_tag="VFB53_10425"
     CDS             35905..36369
                     /locus_tag="VFB53_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZN87184.1"
                     /translation="MAVKKKLKAKARSSSKTKRRKIAKPAAKAVRKPARKAPARRAAP
                     KKAAGPKLAKPRVAKAPPAIPVERPAPGERIGVVTHYYGNPMVAIVKLETGTLRVGDN
                     IHIRGHTSDFSQRVESIQVDHAAVNEAGPQDDFGIKVIEHAREHDVVYKLPR"
     gene            complement(36366..37373)
                     /gene="purM"
                     /locus_tag="VFB53_10430"
     CDS             complement(36366..37373)
                     /gene="purM"
                     /locus_tag="VFB53_10430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZN87185.1"
                     /translation="MSHPLSYRDAGVDIDAGDALVEAIKPYARRTMRPEVLAGIGGFG
                     ALCRIPKKYRQPVLVSGTDGVGTKLKLAFALERHDTVGIDLVAMSVNDVLVQGAEPLF
                     FLDYYACGKLHAAVAAQVVKGIARGCEIAGCALIGGETAEMPGMYPPGEYDLAGFCVG
                     VVERSKIIHGRSIRPGDILLGLASSGAHSNGYSLIRKIVERARPDAALLAALMEPTRI
                     YVKPVLKLLRTVRVKGLAHITGGGLTGNVPRTLPGGTRAVIRKNSWPRPALFRWLQQQ
                     GGVAEDEMYRVFNCGIGMVAVVAAADAKRAAARLRAAGETVYEIGSVEKAKGAPEAVI
                     R"
     gene            37411..38481
                     /locus_tag="VFB53_10435"
     CDS             37411..38481
                     /locus_tag="VFB53_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN87186.1"
                     /translation="MPTPFSANPQFWGWFAIALVAGGLVYLLSPILAPFLFAAILAYI
                     LDPVVERITGKYLPRTLAVLLVMLALLALLAAFALVVLPLFTKELRQLAERLPAFIGW
                     LNQHFAPFLERQLGVEVRFDTDTVRRVAAELVADNEDLVGRLLGTLKIGGLAVLAFLV
                     NLLLVPVVLFYLLRDWHALRARVDRLIPRHAHAKALEILREVDAVLAEFLRGQLLVIA
                     AMSAFYVIALWLARLEFALPIGLITGALVFIPYVGAMLGFVLASIAALLQFDSLWGLA
                     WVWLAFGVGQSLEGMVVTPWLVGERIGLHPVAVIFALLAFGQLFGFFGVLLALPASAA
                     LLVALRHLRRAYLASSLYGGAQ"
     gene            38478..39083
                     /locus_tag="VFB53_10440"
     CDS             38478..39083
                     /locus_tag="VFB53_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZN87187.1"
                     /translation="MTRQLALPISPPPQPTLDNFVPGANAELLARLRQLAAGELAESV
                     LYLWGEPGSGRSHLLAASARPGLTLADDVERLDEPRQIALFNAINAARDAGATVLAAG
                     DAPPAQLALRADLKSRLAWGLVYQVKPLSDAEKALALHAEAARRGLKLSDEVVWYLLT
                     HVRRDLHSLSALLAQIDRASLEQRRAVTLPLVRELIRGLDE"
     gene            39080..39742
                     /locus_tag="VFB53_10445"
     CDS             39080..39742
                     /locus_tag="VFB53_10445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN87188.1"
                     /translation="MTLGLFDLDNTLLAGDSDYEWGQFLVDRGVLPRAEYEAQNAAFF
                     GQYKAGTLDIHEYLGFALRPLAAHTPQDLARWRAEFMRERILPMITPAARALVRRHLE
                     AGELCAIVTATNSFVTAPIAREFGVAHLVATEPETAQGRFTGRVAGTPCFREGKIRRV
                     DEWLAGLGRRLEDFAESRFYSDSHNDLPLLERVRRPVAVDPDPQLAAEAARRGWPVMS
                     LR"
     gene            39756..41081
                     /gene="pcnB"
                     /locus_tag="VFB53_10450"
     CDS             39756..41081
                     /gene="pcnB"
                     /locus_tag="VFB53_10450"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZN87189.1"
                     /translation="MIKRFIRRVLGLPAGAPQRVPRERHGLRREDLSQNALTVCDALR
                     AAGYSASVVGGAVRDLLLRIEPKDYDVATDARPEQVRPLFRRALIIGRRFRLVHVMLG
                     QDTVEVSTFRAADANTAEKDEHGRVLRDNVFGTQEEDARRRDFTINALYFDPATEEII
                     DYHGGLADLKKRVLRMIGDPVTRFREDPVRMLRAVRLAAKLGLSIEAGTRAPIRRLAP
                     LVEHVPPARVFDEMLKLLMSGHASACVRQLREEGLAKGVLPLLDVILEQPLGERFVTL
                     ALAQTDERVRTERPVSPAFLFAALLWHEVLAALKSRQAKGERAQPALEAAMDRVLDAQ
                     CEKLALTRKLTATMREVWALQPRFEQRGGQRPYRLVESPRFRMAYDFLALRAASGEVS
                     PELEAWWRAFQSAGAETRQAMLLPDTGPRKKRRRRRRRKEGAEPGPAPA"
     gene            41078..41557
                     /gene="folK"
                     /locus_tag="VFB53_10455"
     CDS             41078..41557
                     /gene="folK"
                     /locus_tag="VFB53_10455"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZN87190.1"
                     /translation="MTVAYVGLGSNLGDARAHVLRAFEDLARLPETRLEARSALYRSA
                     PLDAPGQADYVNAAAGVDTELSAAQLLAALQDIERRHGRERPYANAPRTLDLDLLLYD
                     EAAFESAMLTLPHPRMHQRAFVLAPLAELDPRLEVPGRGSVRELLARCAGQRVARLD"
     gene            41559..>42058
                     /locus_tag="VFB53_10460"
     CDS             41559..>42058
                     /locus_tag="VFB53_10460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZN87191.1"
                     /translation="MERLRYIAVEGPIGAGKTSLARRLAARLGADLLLEQPGENPFLA
                     RFYRDMARYALPTQLFFLFQRSRMLEPLAQPDMFGRSIVGDFLLDKDPLFARLTLSAD
                     ELALYQKIYDALRPRAPTPDLVIYLQAPSATLIERVRRRAAAFERGIDEEYLALLAES
                     YARFFY"
BASE COUNT         5956 a        15188 c        14539 g         6375 t
ORIGIN      
        1 cgcggccggc gggcgtgcgc gccatgctgc cgcacgtcga cctgttcctg ccggtgcata
       61 atctgaagag cctggtggat ctggcgcata cgctgagccg gccgccggcg cgcaaaacgg
      121 agagcaacgc atggaaatga ccggcgaaga gcgcatcccg gcgtcgcagg cgggcacctg
      181 ggcggcgctc aacgacccgg agatcctcaa ggcctgcgtg cccggctgcg agtcgatcga
      241 gcgcctcgcc gagaacgagt accaggtgct gatggtggcg cgcatcgggc cggtgagcgc
      301 caagttcaag ggcaagctcg cgctgtccga cctgaagccg ccgcactcct acgccatcgc
      361 cttcgagggc cagggcggcg ccgcgggctt cggcaagggc gcggcgcagg tgcagctcgc
      421 gcccgacggc gacggcacca ggctcagcta ccaggtgcat gccaatgtcg gcggcaagct
      481 ggcgcagatc ggctcccggc tggtggacgg cgcggcgaag aagatcgcgc aggaattctt
      541 cgccgcgttc aacgagaaag tgggcgcccg tcacgcgccg gccggggacg cgcacgcgga
      601 gcggcacccg gagccggtgg cgcgcgaccc tgcgctgccg acggtgacgg atgcctcgct
      661 ggtgttcctg gcggcgggct cgctggtggt gttcgtggtg gcgctgttcg cgctgctcgg
      721 ctgaggccgc ccgtcggcca tggcgcgccg cccgtcccgc ccgtccccgc cgggcggcat
      781 tagttcctcc agcgcccgga gcgcgctgcc taggatgcgc gcatcgcccg caacggaaac
      841 ccacgcaagg gagcaagaat aatggcgatg gccacgcagc gcgacaatcc cgcagagcgc
      901 acccccgcgg tgatcctgtt cgccgaaatg cgcaacttca ccagcatgtc ggagatgctc
      961 gacgcggcgc gcgtgctggc gctggccgac cagttcttcg acagcgccgc cgccgcggtg
     1021 caggcgaacg gcggcgaggt catcggcgtg cacaacgact ccctgctggc ggcgttccgc
     1081 cgcggcaagc ccgccgagca gggccgcggc gcggtgcgcg cggcgcagcg catggcgacg
     1141 gacttcgatc cggtggcgca ggcctggcag cgcgactacg gcctgcgcgt ggcgatcgcg
     1201 ctcggcatgc acctcggcga cgccgtgttc ggcgaggccg ggcccgccgc gcagcgcaac
     1261 gacgtggtgt tcggggactg cgtcagcatc gccgagcggc tggtgcaccg ggcgcgcgcg
     1321 ggggaactgg tgctgtcgca cgcggtgatg gcggtggcgc cggtggcgga actcgaactg
     1381 gacgcgcagt cgctgccgcc gctggaactg cacaaccgcc cgccgatccg catctacggc
     1441 gtgctgcgcg acgaccggct cgactttacg agctgatcca ggcgcgcgcc ggcggcacgg
     1501 tggtcgacgc gccgttccac gcctacttcc gcggcatcac ccagaaatag acggtgcggc
     1561 cattggactc cagcaccttg gcggggcgcc agtcgcccat gtcgaactgg tgcgagacca
     1621 cgcgcgtgcc gggcttgagc tgcgcctgca acagcggcag cagcttcagg ttgaggtcgg
     1681 gcagcaggta gagcgtcacc acggtcgctt cgcggaaatc cgcggcgaac aggtcctcgc
     1741 ggcggaactg caccagcttc gccacgcctt gcttcctggc gttgtcgttg gcctcgcgga
     1801 tgcgctcggg atcgatgtcg atgccgaccg cgcgaatgcc gtatttcttc gcagccgtca
     1861 ccgcgatgcg gccgtcgccc gatcccaggt cgtagaggac gtcgcccttc cgcacgtcgg
     1921 ccagccgcag catgtcctcg accacttcct ggggcgtcgg cacgtagatg acatcgggcg
     1981 tgcgcttcgc gtcctgcgcg caggcaaggc ccacgccgca ggcgacgagc agggtcgaaa
     2041 ggatcaggcc atggccgcgc atctcggctc cgcaatgtgc cgggagcggc gattatgcac
     2101 gcccggcgcc gccggcgcgg cgcgcgtcag ggcgagaaac cggcagccca gcggcccgcc
     2161 gcaagcgcct tgtcgaccgc cttttcgtgc ggcgtcctgt ctctctccgg cgacagattg
     2221 cgccatgcag aggctgcgtc cgcgtcgccc gcggtcagcg ccgcttccag gtcgccggtg
     2281 ccatggactt cgatccgggc ccatgagatc aacaggatgg cggccaggat ggcgaggatg
     2341 ctggtccagg cgacgagggg atgttcgtga ccggtatggg gatcgaggga gggggccatg
     2401 gcgcaggaca tgcaagcttc gtgccggttg gacaagccgg cgcgcgcggg gttctgcgga
     2461 ccctattccc cggcgatggt catttcgcgc agcaaaatcg agccgctgcg gcgcgagccg
     2521 cgcaccaccg tgtcgctgcc caccgcctcg atgccccgga acatgtcgcg cagattgccg
     2581 gcgatcgtca cttcctccac cgggtgggcg atctcgccgt tctccaccca gtaaccggcg
     2641 gcgccgcgcg agtagtcgcc cgtgaccagg ttgaccccgt gcccgaggag ctccgtgacc
     2701 agcaggccac ggtgcaggcg cttgagcatg ccgcggaagt cgggcccatt cggcgcgagg
     2761 accaggttgt ggttgccgcc ggcgctgccg gtgctttgca tgccgagctt gcgcgccgaa
     2821 tagcagccga ggaagtagcc ctgcagcacg ccgccgcgca ccacggtgcg cgcgcgcgtg
     2881 gccacgccct cctcgtcgaa gggcgaactg gcgagcgcgc gcggctcgtg cggccgctcc
     2941 tcgagcgtaa tgccgggggc gaacacctgc ttgcccaggc catcgagcag gaagctcgcc
     3001 ttgcggtaca ggttgccgcc gctcgccgcc gagacgaaac tgcccagcag catcgccgcc
     3061 tgcggcgcct cgaacaacac cggcgcctgg cgcgtgccga tgcgacgcgc gccgagccgc
     3121 gccagcgcgc gctcgccggc gtagcgcccg agcgcctcgg gccgcgccag ctgcgccggc
     3181 acccgcgccg cgctgtacca gtcgtcgcgc tgcatgccgc gccggtcctc ggcgatcacc
     3241 gagagataga tcgaatggcg cgacgccggg aaaccgccca tgaagcccag gctgttggcg
     3301 aagacgaact gcgaatgctg cgccgagacc gaggcgccct cggaattgcg gattttcggc
     3361 gacagcgcga aggccgctgc ctcgcagcgc tgcgcaatgc cgatcgcttc ctcggtggcg
     3421 ggcatccagg ggtggaacag gtcgaggtcc ggcagctcgc gcgccaggag ctcggactcc
     3481 ggcggcccgg cgcagtcgtc cgccgcagtc tgccgcgcga ttgcaagcgc cgccgccacc
     3541 gtctgttcca gcgccgccgc ggaaaagtcc gaggtgctgg cgtggccgcg gcgcgccgcc
     3601 ggccgctcgc cgaggtacac gctcacgccg atgcccttgt cgcggttgtg ctcgatggtg
     3661 tccaccgcgc ccttgcgcac ggtgaccgac agcccgtggc cctccgacac ctcgcattcg
     3721 caggcgctcg cgcccagccg cctggcgtgc gccagcacgt ccgccgcaag ctgctgcagt
     3781 tgctcctgcg cgtagccgaa gcggccgccg tttttcatgg cgcgctatca tagcagcgcg
     3841 atgcaggacg aattgaccag caagacgaag aagaaacagg agatgcacgc gctgcagaag
     3901 ctgggcgcgg ccctggtgga gctgtccgcc gcgcatttcg gcagcatggc gctgcccgag
     3961 gccctgcagc tggcggtgca ggaagcgcgc cgcatccgca gccacgaggc caagcgccgc
     4021 cagctgcagc tcatcggcaa gctgatgcgc gggctcgacg ccgagccgat ccgcgcgcag
     4081 ctcgccgccg tgtccggcgg ctcggcgcag gagcgcgcgc gccaccagcg cctggagcat
     4141 tggcgcgcgc gattgatggc agacgacacc gcgctcaccg cgttcgccag cgagcaccgg
     4201 gaggccgacc tgcagaacct gcgcagcctg ctgcgcaacg cgcgcaagga gcagaaggcg
     4261 ggcaagccgc cgcgcgccta ccgtgaactg tttcgcctga tccgcaaggc tgccggtgag
     4321 tgacgcgctg atcgtcgggc tgctgtcggc gagcgaccgc gcgtcgcacg gcgtgtatcg
     4381 ggacgagggc ctcccggcgc tgaaggactg gctgtcgaaa gccgtcctcg ccccggcgct
     4441 gcgctacgag gagcgcctgg ttccggacga gcaggcggtg atcgaggcgg cgctggtcga
     4501 gctggtggac gtgaaaggct gccacctggt gctcaccacc ggcggcacgg gcccggcgcc
     4561 gcgcgacgtg acgcccgaag ccacgctggc ggtggcggac cgcacgatgc ccggcttcgg
     4621 cgagcagatg cgcgccgtga gcctgaagta cgtgccgacc gcgatcctgt cgcgccaggt
     4681 ggccgtggtg cgcaagcagg ccctgatcat caacctgccg ggccagccca aggcgatcaa
     4741 ggaaacgctg gacggcgtgt tcgccgcggt gccctactgc atcgacctga tcggcggacc
     4801 ctacatcgag accgacgagt cgctggtcaa ggcgttccgg cctaaatcgg cgcagcggcc
     4861 gaagccggcg tgacgcagct gcccgaggcg gtcgaagtcg aaaccgcgcc cgcgcccgac
     4921 gccgcggtga tctggctgca cggcctgggc gccgacggac acgatttcga gcccgtcgtg
     4981 cccgagctgc ggctgccggc gcggctgcga ctgcgcttcg tgttcccgca cgcgcccgtg
     5041 cgcccggtga ccatcaacat gggcatgccg atgcgcgcct ggtacgacat tctgcagatg
     5101 ggcggcggcc gcgaggacgc ggccggcatc cgcgcctccc agggcctgct cgaagccctg
     5161 gtcgcgcgcg agcgcgaccg cggcatcgcc gcggcgcgca tcgtcctcgc cggtttctcc
     5221 cagggcggcg ccatcgcgct gcacaccgcg ttgcgccacg ccgagcgcct ggccggcgtg
     5281 ctcgcgctgt cgacctacct gccgctcgct gccaccgccg ccgccgagcg cagcgcggcc
     5341 agccgcgacc tgccggtatt catggcgcac ggctctcagg acccgatgat cgacatcgcc
     5401 cgcgcgcgcg aggcgcgcac gctcctcgag ggactgggcg ccgcggtgca gtggcgcgag
     5461 tacgccatgc cgcactcggt ctgcgccgag gaaatcggcg acatcgccgc ctggctgctc
     5521 agccgtctcg caccatctcc ctgaagcggc cgatctcgta gtcctcgccg cgcgcgccga
     5581 tcccggtcgg catgtagatg tgctgccgcc cgtcgcgcga cgcctccagg ttctggccgg
     5641 gcgcgaatat ccccgggcgc agcgcgatgc gcacttcgtt cgacgcgggg tcgccgccct
     5701 tgagcagcac gccctgctcg gcgctgccgc gagcgccgct cagcgcgaac tgcgacaccg
     5761 cgggcacgcg cgacagggtc tggatgccga cccgcgcctg ctgcgcggcg gtcttgttga
     5821 cgcggcgcac caggccgacg atccagttgc tgccgccatc gggctgcatc gccaccagcg
     5881 cgccgacgcg cagccagtcg cccttcacct gcggcacgat ggcgccgaag ccgcccgcgc
     5941 tcacgttctc gatcacccag ctctcggcgc cggcggcgcc gggcccgccc agggtgtcga
     6001 tcacgccttt caggccgttg ctcacgctca gacgcgactg cacgctgtgg cgctggaact
     6061 tgcgctcggg cggctgcggc gaccagtaca gcaccaggtg ctcgagcacg gcgagcacgt
     6121 cctccggcat ggcgccggga tccaggttga gtcccgccgg caccgcgtcg gtggcgcgga
     6181 tgcgttcggc aagcgccttc gcctcggcaa gcgcggagcc tgcgcccagc cagcgcaatc
     6241 ccggcgcggc gggcggcggt cgcagcgccc gctgcggcgc catcgccttg gccaggtccg
     6301 tccagaccgg caactccgcg gaggcggccg cggcgagcgc gaagcggccg gcgagcccgg
     6361 cgatcaggcg ctcggcgagc tcgatctccg cgggcagcag cccctcgggc gaactggcgg
     6421 cgaacagcgc gccgcgcagg aactcgagct gcggcgtcga cgcgccgggc atgcccggat
     6481 tggcggcgct tgccgtctgc gccagcttgc gcgactcggc gaaggcgtag accttgttga
     6541 aggtggccca cgcggccgcg tcgtagggcc cgtagcgcag gtgcatccat ttgatctgct
     6601 gcgccagcgc ccgcaggcag cgcaccagga gctgcggcaa cgccgcgcgc gccgcatccg
     6661 ctcctttcgc gccttggacg aacaggtcca cggcgcgcgc aaacgcgagg ccggcctcgc
     6721 gccagtagcc gtgcgccgcc agccacatgc ggttctcctg gaagcgcgcc gggcgcgact
     6781 gggccaggta gtcgcgcgtc agccgcgcca ggcgcccctg cgccgcatcg tcgatcgcca
     6841 gcagccgctc gatgcgctgc tcgggcctga agccctcgga ctgcgacacc gactcgtgcc
     6901 attcggcaag ctcctccagc gccttgcacg agtcttgcgc cggcagcgcg tcgagcacgc
     6961 ggcgcgcctc cttggcgtcg gccatcggat ggtcggcgcc gctcctgccg aacagcttaa
     7021 tcatgcgaaa atcgtaacac gctgccccta tgcgcttcct catcgtggac ccttccggcg
     7081 agtttcgcag cacgcttgcc ggcatgctgc gcgcgcgctg gcccctggcc ttcaccgacg
     7141 agtgggatcc gcgcgcgcac ggcagcccgc gcgcggcgct cgcgcgggag cgctatgccg
     7201 cggtgctgat cgccgcgcag gcggagggcg gcgaggagtg ggtcgccgaa atgaggcgcg
     7261 acccggcggc tccgccgctg gtgatgttca agcaggacgg cagcgagagc ctggtgcgct
     7321 cggtggaagg cgtgctgcgc gagcaggaag cgcgccgcgc cgaggtcccc gggaccccgg
     7381 tgttcccgcc ggccgcgcag gccgctgcgc ccaaggccga ctcgccggtg gatcgcgacg
     7441 agcgcgcgat ccccggctac cgcagcctgc gcaagatcgg cgagggcggc atggcgcagg
     7501 tgtatctcgc cgagcgcgag caggacggcc tgcagctggt gctgaaggta ctcgaccccc
     7561 gcctgcgctc cgaccagacg ttcttcaagc gcttcgtgca ggaataccgg ctggtggccg
     7621 gactgcacaa cgagtacgtg gcgcacatct acgaccaggg ctttgccggc gagcacccgt
     7681 acatcgccat ggaattcctg tccggcggca cgctcgccac gcgcatccac gaaggcgtta
     7741 cttcgctcgc ggcgctgcgc attacctcgc agatcgcgcg cgcgctcgac gcgattcacg
     7801 cccacggcat catccatcgc gacctcaagc cgcagaacat cctgttccgc gacaacggcc
     7861 gcccggtgat cgtggatttc gggctggcca agaacctcgg cgcgaagacc acgctcacgc
     7921 ggcacggcca ggtgatcgcc acgccgcgct acatgagccc cgagcagtgc ctgggcaagg
     7981 ccgccgacca tcgctccgac ctgtacagcc tgggcgtcat cttctacgag atgctgagcg
     8041 gcctgaagct gtacgacgcc gaggggcccg caggcctggt gtaccagcac gtgcacgggc
     8101 cgatcccgcg gctgccggga cgcctggcgg ggtaccaggg catcgtcgac cgcttgctcg
     8161 ccaagacgcc cgaggcgcgc ttccagagcg cgcgcgagct gttcgcgatg atcgcggtat
     8221 gagcgccggg gcgggcgagc ggcagtacct cgcgctgctg gaggaagtgc tggcgcgcgg
     8281 cgcgcccaaa tccgaccgca ccggcaccgg cacgcgttcg gtattcggcc gccagctgcg
     8341 cttcgggctg gcgcaggatt tcccgctgct caccaccaag aagctgcacc tgaaatccat
     8401 cctctatgag ctgctctggt tcatgcgcgg ggacaccaac gtgcgctggc tgcagcagca
     8461 cggggtctcc atctgggacg agtgggcgga cgccgacggc gagctcggcc cggtctacgg
     8521 ctaccagtgg cgccactggc gcacgccgga gggacgcgag atcgaccagc tgcgccaggt
     8581 gatggagcag atcaaggcgc ggcccgactc gcgccgccac ctggtgaccg cctggaatcc
     8641 gggggatatc gaacgcatgg cgctgccgcc ctgccatgcc ctgttccagt tctacgtcgc
     8701 cgagggctgc ctgtcctgcc agatgtacca gaggagcgcc gatttgttcc tcggcgtgcc
     8761 cttcaatatc gcctcttacg cgctgctcac cctgatggtg gcgcaggtgg ccggcctacg
     8821 gccgggcgag ttcgtgctca cgctgggcga tgcgcacctg tacctcaacc acctggagca
     8881 ggcgcgcgag cagctgcggc gcgagccgcg gccgtttccg cgcatgcgcc tcaacccggc
     8941 ggtgcgcgac ctgttcgagt tccgctacga ggacttcacg ctggaaggct acgacccgca
     9001 cccggcaatc aaggcgccga tcgcggtata aaaaaaaggg gcccgtctgt agggggatag
     9061 atcgggcccc gggggaggaa ctgctctgct gccggtcgtg gagggctcta gaagctcccg
     9121 aggaccagca actggccgag gaacaacacc ccggccgcgg caaaactgta gaccaccgcg
     9181 tcggcgatgc gggcgcgctg tcccggcgcg aagccgggca ccgtccacac cactgcggca
     9241 gccccgagca gcgcggcgag caaggccgcg ccgaaactga aactgtgccc gaaggccagc
     9301 gcgatccagc catcgggcga aaccagcttg tagatcgacc ctgcgatgcc aaccatcacg
     9361 agaacgctga ccatgaaacc gatcagcacc tgcgccagga tcggtacttc gaacttggta
     9421 tttgcctgtt ccatatccat tgacaatctc ttgcaaccat cgtgccagga cagatgaata
     9481 tatagatatc aatgggttag gaattcagcg tgaccaaagt tacggccgcg aaacggcaaa
     9541 aaccgtctcc ggggcacgac gaaccacatc acattcaggc caagagcctg acagcgcgcg
     9601 gcttttcctg tcgcctggcg gcgacagcct caggattcgg ccgccacctt tatcttgaac
     9661 attgccggct ccagcgcgtg gcgctcgagc agccggtaga actcggtgcg gttgcgcccc
     9721 gcgaggcgcg ccgcctgggt gacgttgccg cccgtgatct tgaggatgcg caccaggtac
     9781 tcgcgctcga atgcgcgccg tgcctcgtcc agcggcgtaa gcgatccggc gcccgcgtcc
     9841 agcgcctggc ggaccagcga cgcgggaatc acgtcggtcg ccgtgagcgc gaccgctcgt
     9901 tcgatgacgt tgagcagctg gcgcacgttc cccggccagg cggcggagac cagcacctcc
     9961 atcgcctcgg gcgacatgcc caggcgcggc cggtcgtagc gctcggcgaa gcgcgcaagg
    10021 aaatgcccgg cgagcagcgg cacgtcctcg cgccggcgct ccagcgccgg cagttcgagc
    10081 ttcaccacgt tcaggcggta atacaggtcc tcgcggaact cgcccgaggc gatgcgctcc
    10141 tcgagattgc ggtgcgtcgc ggaaacgatg cgcacgtcca ccgccaccga gtcgttggcg
    10201 cccaccgggc gcacctcccg gtcctcgatc gcgcgcagca gcttgacctg cagcgccggc
    10261 ggcatgtcgc cgacctcgtc gagaaacagc gtgccgccct cgcaggcctg gaacagcccg
    10321 cgccggtcct gcactgcgcc ggtgaatgcg cctttcttgt ggccgaaaag ctccgactcg
    10381 agcagcccct cggggatcgc gccgcagttg acggcgatga acggcgcgcc ggcgcgccgg
    10441 ctggcgcgat ggatggcgcg cgccagcagt tccttgccgg tgccgctggc gccgaagatg
    10501 cagacgctgg cgtcggaagc cgccacgcgc tgcgcctggc ccagcagggc ctccatggtg
    10561 gccgcgcgcg tgatgatctc cgagcgccat tgcgcgcggt cgccgctggg cgccgtcgac
    10621 aggcgcagcg cgtcggcgac ggtttccagc agcactttcg gctcgaacgg cttggtgagg
    10681 aagctgaaca cgccgcggcg cgtcgccgcc accgcgtccg ggatggtgcc gtgcgccgtg
    10741 aggatcacca ccggcaggga aggcgcctgg cggtggatgg catcgaacag cccgagcccg
    10801 tccatgccct gcatgcgcag gtcggtgatc accagttgcg ggcgctgcac cgcgaggctg
    10861 gcgagggcgg cgtcggccgc ctccaccgcc gtgacgcggt agcccgcggc ggtcagccgc
    10921 atcgacagca ggtgcagcag gtcccggtcg tcgtcgacca gcagaatgga tggcgcttcc
    10981 tggttcatgg cttcacctct tgattgtcct gcggcgttct tcgcgctcga ggataccgcg
    11041 ctcgatcgac ttcaaggctt cgacctgctg gcgcagcgtg gtggcgcgct cctcggccgc
    11101 ctcctcgcgc ttctccgccg cgcgcaggtc gcgggcgagc ttctggcgct cggccacgcc
    11161 cgccgacagc agcgcggcca gttgcaccag cggcgtttcc gggcggcgcg ccgccagcgg
    11221 ctcgagcagc gcgaccgcgc gggcgtcgtc gcgcatcggc gccggcaggt tcgcgagcag
    11281 cgccgccaga cgcacgcggt tgccgtcgtc cggcacggcc gcgaacatct gttgcgcgtg
    11341 cgtcaactgg cggcgctgct cctccggcgc cgcgcgcgcg ccgctcgcgg cggcggcgac
    11401 gatgtcgcgc acctgcagcg cttcgccggc atccggctgc agcagggcgc agcccgacag
    11461 cagtgcggcg cacaggacgg tggccggcct catgcggcct ccgccagcac cagcggcgcc
    11521 ggccgcttgg gcagagtgac gcggaaatgc ccgcccgggc cctccgccac cagcgcgatg
    11581 cgcccgccgt gcgcctcgac gtactcccgc gcgatggcca ggccgagccc gctgccctcg
    11641 acgcggccgc cggccttggc gcggccgcgg aagaaggact cgaacaccgc ctcgcgctcg
    11701 accgccggga cgccgggacc ggaatcgatc acctcgacca ccacgccgcc ttcggcttcg
    11761 cgcgtggtga tcgagatgct gccgcccgcc ggcgtgaact tgatcgcgtt gcccagcagg
    11821 ttgtcgatga tcgagcgcag cttctcggcg tccgcctcga ccgccaccgg cgccaggtcc
    11881 agcgccacgc gctgcgcctt ggccagcgcc gccagccggt gcgcgtcggc cacctcgcgc
    11941 gccagcgtgt cgagccgcac cgtgcgcacc tcgagcgagg ccgccgcgtg cagcgcccgc
    12001 tggtaatcca gcaggtcctc gatcagcttc tgcagtttga tgctgttgtc gcgcatgatg
    12061 cccaccaccg cgcgctgcgg cggcgcgagc ggcccggcga cctggtcgtt gagcagttcc
    12121 gcgccctcgc gcaaggaggc caggggtgtc ttgagttcat gggacaggtg gcgcaggaat
    12181 cggttcttct gcgcttcgag ctcggtgagg cgctggcgca accaatccag ccgctcgccc
    12241 aggtcgcgca ggtcctgcgg cccgctgacg cggatcggcc gggagaagtc ggccccgccc
    12301 agctgccgga tggaggcgtc gagctcggcg atcgggcgcg cgatgatgcg cggcagcgcc
    12361 agcgcgaacg ccagcgccac ggcaatggtg gccagcacca gctgcagcag gcgccgctgc
    12421 accacttcgg cggccgcgcg cagacgctcg acctcgccgt cggcgacgcg gtagctgatg
    12481 gcctgcactt catgcgcgct ctcggccagg tcggacactt tctcgttcac ggcgcgggca
    12541 tccggcgggc tgcccgaggc gcccgacagc agctcgtaga gggcctgctc ctcgccgatg
    12601 gtgcggcgga gcgcggtcag ctgctcgccg tcgagcggca ggcgcgccag ttcgtccgac
    12661 agctgcctga accccgcgtg caggcgctcg tagtcctgca ccaggcccgg atcgggcagc
    12721 agcgcgacct gctgcgccag acgctcgatc gacgcgatgc ggttcaccag cgcccggctg
    12781 ccgcgcgccg cctgcgccgc gttgtacacg gcgctgcggc tcagctcggc caggcgctgc
    12841 gtattccatg ccgagtacac cagcgcaccg gcaagcggcg ccgcgacgag gaggaatccc
    12901 gtcaccagca aaccgaggaa ggatcgcgga aaacgcaggg ccgcccccgg ggaggtgtcc
    12961 tgaggtgcca tgtcactata atctcacgta aaacaatgtc aggacgagcc atatgtcaca
    13021 ggccgtagca cggccctgcg cgacgcgcct gtatttggtc gcggcggtgg cggccaacgg
    13081 ggtcatcggc gcgcacggca agcttccctg gcacctgccc gaggacctgc ggcacttcaa
    13141 ggcgctcacc ctgggccatc cggtggtcat ggggcgcaag acctgggagt cgctgggccg
    13201 cgcgctaccg gggcgcgaaa acatcgtggt gacgcgcgcg cccggctacg cggcgcccgg
    13261 cgccagcgtc gcctcgtcgc tcgaggcggc actcgcgctc tgcgcgggcg agccgaccgt
    13321 gttcgtcatc ggcggcggcg cgctctatgc cgaggcgctg gcgctggcgg acggcctggt
    13381 gctgacggag atccagcgcg actacgccgg cgacgcgcgc tttccggact acgaccgggc
    13441 ggcctggcgc gagacccagc gcaagccgca gaccggcgcc gacggcctgc gcttcgactt
    13501 cgtgctgtac gaaagggcct agcgcgagcc ttgccctact tcgtcctgga ctcgaacacg
    13561 cgcacttccg gcagcggaaa gccgttgaac tcgctggcgt aggccgtggt gtaggcgccg
    13621 gcgttgcgca ggtagatgaa atcgccttcc tgcaggtccg acggcaacgg ctcgtcgcgc
    13681 attaccacgt ccaccgagtc gcaggtggga ccggccacgt tccaggcgac gtccggcccc
    13741 gagcggtcgg tgcggatctt gtacttcagg ccttcggtgg tctcgatcac gccgccgaac
    13801 aggcccgcgt cccagtgcaa ccagcgcttg ccggcgcgcg tcgcggtgcc gaggacgcgg
    13861 cacacgaagt agccggcatc cgacaccagg tagcggcccg gctcggcgat cacctgcacg
    13921 tcctcgggga atgccttcag cgcttcgttc gccacttcgc cgatcacttc gatcgaggga
    13981 atcggcttga cgtggcgcac cgggaagccg ccgccgatgt ccagcagccg cggcttgagg
    14041 ccggccttga gcatgccgtc gaacagggtg cgcgcctttt cgatgccgac gcgccagttc
    14101 tcggggttgc ggcactgcga gccggcatgg aaggtcaccc cggcgagatc cgcgccgagc
    14161 ttcgccgcgg tggcgatgat ttcgcgcgtg tcgccggcgc cggcgccgaa cttgcccgac
    14221 agcggccagt cgctgccgat gttcggggtg gcgatgcgca ggtaaagatg cgcgctcgga
    14281 tccaccgcgt gcatgcggcg cagctcgtcc acgctgtcga ccacgaacca gcgcacgccc
    14341 ttggcgacgg cgtaggcgtc ggcatcgcgc gagcgcatcg ggttggagaa gaacacctcg
    14401 gcggcgttca cgcccaggga cagcagcagg tcgagttccg cggtcgaggc gatctcgaag
    14461 cccgcgccct cctgcaccag cgccttcagc acgcgccggt cgggattcgc cttcaccgcg
    14521 tagtgcgggc gcacgcgcgg catggcggcg cggaagcggc gcagcttttc gcgcacgatg
    14581 gcgctgtcga tgatcaggaa gggccgcgtg tacttctgcc gtgcggcgcg gcgcacgatc
    14641 tcgaagtcga ggctgacttc ggcgtgcgtg tcgaagagct gggattgcgg aagcgaggac
    14701 tgctgtcggt tgcgtacgag gctgctgggg cgatcgccca tcaatgttct cccgggggca
    14761 tctaggtctc agggatgtct cgaactgcga cgtcgtacac cgggcaggag ttcctctggc
    14821 tagtcatgat gacttcccct ttctccatgg tccgttgcgc ccatattccg ttgagtcaaa
    14881 agtaaaactt cggggcgatt atagggatga ccccccgcca gtcaagggct ttttggcggt
    14941 tttttcggtc gccgcgctgg gcgcgggcgg acgcctgcct tgagaaacca ctataggtag
    15001 gtgcgctaac cgaccgtgct actagggatt gcggatcaga tgcgaggcag ggtcacgccc
    15061 tgctggccct ggtacttgcc gccgcggtcg cggtaggagg tctcgcagac ttcgtccgac
    15121 tcgaggaaga tcacctgcgc gcagccctcg ccggcgtaga tcttggccgg cagcggcgtg
    15181 gtgttggaga actccagcgt cacgtgcccc tgccattccg gctccagcgg cgtgacgttg
    15241 acgatgatgc cgcagcgcgc gtaggtggac ttgccgaggc agatggtgag cacgctgcgc
    15301 gggatgcgga aatactcgac ggtgcgcgcc agggcgaagg agttcggcgg gatgatgcac
    15361 accgcgccct tgaagtcgac gaacgacttc gcgtcgaagg ccttcgggtc gacgatggtc
    15421 gagttgatgt tggtgaagat cttgaattcg tccgagcagc ggatgtcgta gccgtaactc
    15481 gaggtgccgt aggacaccac ccgctggccg ccggcctctt tgacctggcc cgcctcgaag
    15541 ggttcgatca ttccctgcgc cgccatgcgg cggatccact tgtcggattt gacggtcacg
    15601 tattctgcac gacgatgttg ggaaacttgc cgctcatgtc cttctgcgac tcggcgatcc
    15661 tgaccgcgca gcggcgcgcg atgcgccggt agatctgcgc tgccttgccg tccggatcgg
    15721 cgaccaccgt cggcctgccg gaatcggcct gctcgcggat cgactcgtcg agcggcagct
    15781 ggccgaggag ctcggtgccg tagtcctggc acatgcgcgc ggcgccgccg ctgccgaaga
    15841 tgtggctctc gtggccgcac ttgggacaga cgtggatcga catgttctcc acgatgccga
    15901 ggatcgggat gccgaccttc tcgaacatct tgaggccctt gcgcgcgtcg atcaacgcga
    15961 tgtcctgcgg cgtggtgacg atcaccgcgc cggtcaccgg caccttctgc gccagcgtca
    16021 gctggatgtc gccggtgccc ggcggcaggt cgaccaccag gtagtccagc tcgcgccagc
    16081 gcgtgtcctt cagcagctgc tcgagcgcct gcgtcaccat cgggccgcgc cacaccatcg
    16141 gcgtgtccac atcgatgaga aagccgatcg aaatcgcctg caggccgtgc cccatcatcg
    16201 gctccaggcc cttgccgtcc ttggattcgg gccggccggc gatgccgagc atcgtcggct
    16261 gcgacggacc gtagatgtcg gcatccagga tgccgaccgt ggcgccttcg gccgcgagcg
    16321 ccagcgccag gttgaccgcg gtggtgctct tgcccacgcc gcccttgccg gaggccaccg
    16381 cgatgatgtt cttgatcccc ggcaccagct tcacgccgcg ctgcaccgaa tgcgacacca
    16441 ccctggacga gacgctcgcg gcgacgccgc cggcgccggc cttcttcagc gccgcggtga
    16501 tctcgcgctt gatcggctcg aactggctct tgcccggata gccgagctcg atgtccacgc
    16561 gcacctcggc gccgtcgacc ttgacggcct tcaccgagcg gctgctgacg taatccttgc
    16621 cggtgttcgg gtcgatcagc tgcgagagga cggcgcggac ctcggtgtcg ctcacgggca
    16681 tggcgcaagt gtaagctaaa atccgcgttt tcctcgggcc cgcaatgccg cgacgcaggc
    16741 tgttcgtcac caccgcgctg ccctacgcca acgggccgtt ccacatcggc cacatcatgg
    16801 agtacatcca ggccgacatc tgggtgcggt tccagcgcat gcagggccat gcggtgcatt
    16861 tcgtcggcgc cgacgacgcg cacggcgcgc cgatcatgct ggcggcggag aaggccggcg
    16921 cggcgcccga ggcgttcgtc gcgcggatcg ccaagggacg caagcagtac ctggacggct
    16981 tccacatcgc gttcgacaac tggcactcga cgcattcgcc ggagaacacc cagctgtcgc
    17041 aggacatcta ccgccggctg aagaaggccg ggctggtcta caccaagccg gtggagcagt
    17101 tctacgaccc ggtgaagggg atgttcctcg cggaccgcta catcaagggg gaatgcccca
    17161 agtgcggcac gcgggaccag tacggcgacg cctgcgagaa ctgcagcgcc gcgtactcgg
    17221 cgaccgacct gaagaatccc tactcgaccc tgtcgggcgc caagccggtg ctgaagactt
    17281 ccgagcacca tttcttccgc ctgtccgacc cgaaatgcag gaccttcctc aagggctggc
    17341 tggagaagcc cgggcgcctg caatcgcagg tggtgaacaa ggcgaaggag tggctcgacg
    17401 gcgaaggcga caaggcgctg ggggactggg acatctcgcg cgacccgccc tacttcggca
    17461 tccgcgtgcc cgacatcgcg gaggagaaat acctctacgt ctggctggat gcgccgatcg
    17521 gctacctcgc cagcttcaag aactactgtg ccaggacggg gatcgacttc gagaaattcc
    17581 tgcaggaccc gggcacggaa caggtgcact tcatcggcaa ggacatcatc tacttccaca
    17641 ccctgttctg gccggcaatg ctcgaatacg cgggcaagcc ctacaaggtg ccggaccatg
    17701 tctacgtgca cggcttcatc accgtgtccg gcgacaagat gtccaagtcg cgcggcaccg
    17761 gcatcagccc gctgcgctac ctcgagctag gcatgaaccc ggagtggctg cgctactaca
    17821 ttgcggccaa gctcaacgcg aacgtcgagg acatcgactt caacccggac gacttcgtcg
    17881 cccgggtgaa cagcgacctg gtgggcaagt acgtcaacat cgccagccgc gccgcgagct
    17941 tcatcgccaa gcacttcgag gggaagctct acgacttcgg cagcgaccct gcgccgtcgt
    18001 gggcggccgc tgtggcaatc gacgatgaga tcgccgccgc gtacgaggcg cgggagttcg
    18061 gcaaagtcgt gcgcgaagtc atggcggtcg ccgaccgcgt caatcagcgg ttcgaccagg
    18121 ccaagccctg ggaactcgcc aaggatcctt cccgcatcgg ggatctccat cgcgcctgca
    18181 gcgatgccct ccaggcgttc ttgaccctga cggtctacct cgcgcccatg ttgcccgtca
    18241 ccgccgtgcg cgtggcgcgc gagctgttcg ggctggaacg ggatcttctg ttcgacgatc
    18301 gcaagctgcg cgcgacggcc atccggccat tcaagcacct ggcgacccga gtggaagagc
    18361 ggcagctcga ccagctcttc gacatcgccc cagcccccgc acccgccgcc aaggagaagt
    18421 ccgtgtccga cccgatcacc atcgacgatt tcaacaagct cgacctgcgc gtcgccagga
    18481 tcgccaaggc cgagcacgtc gaaggcgccg acaagctgct caagctcacc ctcgacctcg
    18541 gcggcagcac gcgcaccgtg ttcgccggca tcaagtcggc ctacgacccg gccggactcg
    18601 aaggaaaact ggccgtggtg gtcgccaacc tcgccccgcg caccatgaaa ttcggggttt
    18661 ccgagggcat ggtgctggcc gcctcggggg cggaaccggg aatattcctc atttcgccgg
    18721 atgccggggc caccccgggc atgcgcgtaa aatagccgcg ccgcagcccc tgccccgcca
    18781 caggctcgac gcatgatcga acagcccagc cgcacgctgg tttgcagcat cgtgttcctg
    18841 gacatcgtcg agtactcgac gaagccggtg gcggagcaat tggtgctcaa gcaggccttc
    18901 aaccgcctgc tcagttcctc gctggagtac gtgccggcgc gcgaccgcgt ggtgctggat
    18961 accggcgacg gcgccggcat cactttcctc ggcgacccgg aagatgccct gttcgtcgcc
    19021 atgtcgctgc gcgccgcggc gcgcaccgag gccaccggcg ccatcgccat ccgcatcggc
    19081 atcaacctcg ggccggtgcg cctggtgaag gacatcaacg ggcagatgaa catcatcggc
    19141 gacggcatca acgtcgcgca gcgcgtgatg accttcgccc aatcgggaca gatgctggtg
    19201 tcgcgctcct tctacgaggt ggtgtcctgc ctgtcgctcg actacgccag cctgttcagc
    19261 tacgtcgggg cccgcaccga caagcatgtg cgcgagcacg aggtgtactc ggtgggcatc
    19321 ggcaccgacg cggcgcgccg cgtgctcgaa accggttccc ggcgcgtggc gccccgcggc
    19381 agcgcgccgc ggctgatcgg cgagctcacc gacgtgcggc cgttcggcgt gcatcgcatg
    19441 gcgctgctcc tcacgccgct gctgttcgcg ctcctggtcg gcggcgggtt cgcggtgcgc
    19501 gcccaggtgc gcccggccga agcccaggcg ccggcggcga aggcgcagcc gcccaagcct
    19561 gcgctgacgc ccgtggcggc gaaggccgcc gccgccccgg agaagcccgt ggccgtggcg
    19621 ctcgcgcaac ccgcggcgaa agccgcggcg ctccagccga aggcggagcc ccagccggcg
    19681 gcaccgaagc ccgtcaagac gctgacgcct gcccagaagg aggcgcggcg cgaggcgctg
    19741 gccgccaggg cggcgcgcgc gcaagctgcc aagaccgagg ccgcacaacc ggcccaagcc
    19801 gccgcgtccg tcgcgaccgc cagcggcacc gtgcagctcg cgatcctccc ctggggcgaa
    19861 gtgttcgtgg acggcaagcg ccgtggcgtc agcccgccgt tgcgctccat cgaagtgccc
    19921 cccggcacgc acacgatcga ggtgcgaaac acctcctttc cgtcccatgc ccagcgcgtc
    19981 gaggtgcgcg tcggcgagcc ggtgcgcatc aggcatcact ttcgctgatc gcccggtcgc
    20041 ggtatcttct gcctctgggc aggggggatc ggcagacgtg ggcaggcgcc agttcggcaa
    20101 ccaagcatcg acgcgcatcg cgatcctcgc ggcgctggcg gccgtcgcct tgctcgccgg
    20161 ctgccaatcc gagccgatcc gcggcctgca ggccgacttc aagggcctgt tcggcaaatc
    20221 caagggtgag cccgcactcg ccgccggcct gcgccagtac gaggacggca actacgccga
    20281 ggccgccaag cagctgcaaa gcgcgatcgg ccagggcctg acgagcaacg aacgcgtcca
    20341 ggcgcacaag caccttgcgt tcatccactg cgcctccgat cgcaccgccg cctgccgcga
    20401 ggagttccgc cgggcgctgg cgatcgatcc gggcctggag ctgtccgccg cggaagccgg
    20461 gcatcctagc tgggggccgg tgttccgcgc gctcaagacc ggccgctagc cgccagctgc
    20521 cgcgcagatg gaaaacacca agctcggccg gttcgaaatc gtggccgaga tcgggcgcgg
    20581 cgcgatgggc atcgtctata aggcgcagga cccgatgctc gagcgcaccg tcgccatcaa
    20641 gaccatcaac atgggcatgg accgcgatgg cgcggagatg tacgagaagc gcttctacca
    20701 ggaggcgcgc gccgccggcg gtctgaacca tcccaacatc gtcacggtgt acgacatcgg
    20761 caagaccgag accgcctgct acatggcgat ggagtacatc gagggagccg aactgcgcac
    20821 cctgctgctg cccggcaagc ccctcccggt ggcgcgcgcg ctgtcgatcg cggcgcaggt
    20881 ggccgagggg ctggcgtacg cgcacgagcg cggcgtggtg caccgcgaca tcaagccggc
    20941 gaacatcatg gtgccggaga gcggcgcggt gaagatcacc gatttcggca tcgcgcgcat
    21001 gcgcacctcg aacgtgcaga cgcagaccgg catgatgatg ggctcgccca agtacatgtc
    21061 gcccgagcag gtgatcggca aacgcgccga ccaccgctgc gacatcttct cgctcggcgt
    21121 gatcctgtac gaaatgctga ccggggcgtc gccgttcacc ggcgagagcg tcaacgcggt
    21181 catgtaccag atcgtgaatt tcgtgccgcc ggcgccgagc gccatcaacc cggctgcccc
    21241 cgcggcgctc gacggcatcg tcgggcgcat gctcgccaag gcgctggagg aacgctacca
    21301 gagcgccgcg gaaatcgcgc gcggcctgcg cgattgcgag caccagatgc gcgacgcggg
    21361 cggcggcgct gcgccatcgc ccgacaccgc cagcaccgtt ccggtctcgg cgctcgcgcc
    21421 ggggccggcg cccgcggtgg tggacaccca ggtcaaggca gcggtgctgg cgcagaccgt
    21481 ggagcgctcg cggcgcgccg aagaggagac gccgtcgcct gacgccgcgc cggcgcgcgg
    21541 cctgtcgcgc accttcgatt ccctcgaggc cacgcagcgc ctggtggcgc tgagcgacgc
    21601 cgccgtgccg agcgaccccg cggcgagcac cttgccgctc accatgcaga ccacggccat
    21661 ggtgatggcc ggcggctgga agcaacgcga cacggtcgtc gtcgctgcgg gcgccgtcgc
    21721 cggactgatc gtcgcggcgc tcatcgtgtg ggcatagagg agtcgggggc gctgtccgcc
    21781 agcctggcgg ccgccgccgc ctggatcgcg atcggcgcgg ctggcctgtt gcggcccgcc
    21841 agcctggtcc tcgccagccg cgtgctgttt cccgccggcg cggcagtggg atgcgccctc
    21901 gccgcctggg cgctgcaggg cgtgttcgcc acgccgcagg ccctggtgct gccgctgggc
    21961 ctgcccgacc tgccgttcca cgcgcgcatc gacgccctgt cggcgttctt cctgctgctg
    22021 ctgggagcgg tggcggcggg ggtgtcgctg ttcgccagcg gctacctgcg cccgggccag
    22081 ggcacttcgc ccgggctgca gtgcctctac taccacgtgt tcctcgccag catggcgctg
    22141 gtgctgatcg ccgacgacgc ctatttcttc atggtggcct gggaaaccat ggcgctcgcc
    22201 tcctacttcc tggtcaccag cgagcaccgg cacgaggaga tccgccgcgc gggctacctc
    22261 tacctgctga tggcgcacat cggcgcgatc gcgatcctgc tgtgcttcgg cgcgctgcag
    22321 gcgggaaccg gcgactacac ctttcacaac atgcgcgagc tgcagccggg cgccgaggtg
    22381 gcgacggcgg cgttcctgct ggcgctggtc ggcttcggcg tcaaggccgg catcgtgccg
    22441 ctgcacgtgt ggctgccgga agcgcacccc gcggcgccct cgccggtgtc cgccctgatg
    22501 agcgcggtga tgctgaagac ggcggtgtac ggcatgctgc gggtgtccct cgacctgatc
    22561 gcgtttccgc tctggtggtg gggcgtggtg gccctggtgc tcggcctggt ctccgcgctc
    22621 ggcgggatcg tgttcgccgc ggtgcagacc gacatgaagc ggctgctcgc ctattcctcg
    22681 atcgagaaca tcggcctggt gttcgccggc ttcggcctga ccctcatctt ccgcagcttc
    22741 ggcatgggca cgctggcggc gctggcgctg acggcgacgc tgtaccactg cctcaaccac
    22801 gcctttttca agtccctgct gttcctgtgc acgggctcgg tgctgcatgc cacgagcgag
    22861 cgcagcctgg gcaagctggg cggactgatc caccgcatgc cctgggtggc gtggtgcgcg
    22921 ctcgtcggca gcgtggccat cgccggcctg cccccgctca acggcttcgt ctccgaatgg
    22981 ctgctgctgc aggccttcct cctcacgccg gggctgccgc aggcctacct caacatgctg
    23041 gtgccggtgg ccgccgccgt ggtcgcgctc accgccgcgc tgtcgggcta cgtgatggtc
    23101 aagttctacg gcgtggtgtt cctcggccag ccgcgcgacg cgcgcctcgc cgaggcgcac
    23161 gatgcgggcg cctgggagcg caccgggctt gcctggctcg ccgccatgtg catcgtgctc
    23221 ggcctgttcc cggcggcggt gatcgggctg ctcgacccca ttacccgcgc gctcaccggc
    23281 cacggcatgg ccagcctggc gcagggctgg ctgttcctcg cgccgctgca gcccgagcgc
    23341 gcgagctaca gcgcgctgct gtttttcgcg gtgaccgggc tgctggttgc gctcacctgg
    23401 ctcgcggtgc gcgccttcta tcacggccgg gtgcggcgcg ccccgccctg ggactgcgga
    23461 tttccgctgc agaacgcgcg catgcaggac tccgcggagg ggttcggcca gccggtgcgc
    23521 catgtgttcg agccgttctt ccgcgtcgag cagagcgcgc cggcgccggc ggacgcgcga
    23581 ccgtcctaca ccgaggtgca cgacgaccgc ctgtggcact ggctgtacct gccgctggcg
    23641 cgcggcgcgg accgcctgac cgggctggtc accttcctgc agcgcgggcg catctcggtc
    23701 tacctcagct acagcttcgc cacgctgctg gtgctgctgt tgttcgtcgg ctgaggaccc
    23761 atggccagcc tgcaggggct cgtcgcccag ttcgccgtcc tggcgctcac cgtgctgctc
    23821 gccccgatgc tcacgggctg ggtgaaccag tgccgcgcgt ggctgctgaa ccgcagcggc
    23881 ccgggcctgc tgcagcccta ccgcgtgctg gccaagctgt tcagcaagga ggtggtgctg
    23941 gcgcacaacg cgagcccgct gttccgcggc gccccgtatg tgatcttcgg ctgccttgcg
    24001 ctcgcctccg ccatcgtgcc gaagatcggc accgacctgc cgctcaatgc cgcggcggac
    24061 gccatcgcgc tggtcgggct gctcgcgctc gcgcgcctgt tcaccgcgct cgccggcatg
    24121 gacatcggca ccgcgttcgg cacgctgggc gcgcggcgcg agatgctggt ggggttcctg
    24181 gcggagccgg cgctgctgat ggtcctgttc acggcctcgc tcatctcggg ctccacgtcg
    24241 ctcgccacca tcgtcgaggt gctcgcgcat cgggaattcg tgatctaccc cagcctcgcc
    24301 ttcgccggcg tcgccttcac catggtgtcg ctggcggaga acgcgcgcat cccggtggac
    24361 aacccggcga cgcacctcga gctgaccatg atccacgagg cgatggtgct ggagtactcc
    24421 ggccgccacc tggcgctcat cgagtgggcg gccagcctga agctgctcgt ctattcctgc
    24481 atcggcctgg cgctgttctt ccccttcggc atcgccgagc gcggcgactg gcgggggctg
    24541 ctgctcgcgc tgccggtgct ggcgctcaag ctcgctgcgg gcggcttcct cctcgccctg
    24601 atcgagacgc tgtgcgcgaa gatgcgcatc ttccgcgcgc cggaattcct cgccaccgcc
    24661 ttccttctgg cggtgctcgc catgctgctg cacgtgctgc tgggcggctg aggtgctgca
    24721 gcactacggc ccccagctgg tcaacttgct cgcggcggtg ctgctgctgc tgtccttcgc
    24781 catgctcgcg cagcgcagga tcctctcgct catcaacctg tacgcggcgc agggctgcgt
    24841 gctgttcctg tcgaccgcgc tggtggcctg gctcaccggc cagaaccacc tgtacggctc
    24901 ggccgcgctc acgctcgcgc tcaaggtgat cgccctgccc tggatcctgc accggctgat
    24961 ccggcggctg aacgtgaagt gggacgtgga gacgctgatc aacattccgg ccaccatgct
    25021 ggtgggcatc gtgctggtga tcctcgcgtt caatctcgcg ctgccgatct cgcagctcgc
    25081 cggcacggtc acgcgctcga ccttcggcat cgcgctggcg agcgtcctgc tctcgtttct
    25141 catgatgatc acgcgctcca aggccgtgcc gcaggtgatc ggcttcctgg cgatggagaa
    25201 cggcctgttc ttcgccgcca ccggcgccgt gtacggcatg ccgatggtga tcgagttcgg
    25261 cgtggcgctc gacgtcctgg tcggcgccct gatcctcggc atcttcgtgt tccggatccg
    25321 cgagcgcttc gactccgtgg acacgcgcca cctcgaaaag ctgaaggagc gctgatggcg
    25381 ccggagatcg cgctgctgct cgcttcgcct ctcgccggcg gcctggcgct cgccctgttc
    25441 ggccaccgcg accgcgccgc ggagctgaac atcgccttca gcctgctgac cttcgtcgcc
    25501 gctgccgcgc tggtggcgcg cgtgggcgcc gaggggccgc tgctcgcggc cagggcgcag
    25561 ttcttcgtcg actcgctcaa cgtgttcctg gtggcgctga ccgcattcgt ctcgctcacc
    25621 accgcgctgt tctcggcccc ctacatgcgc gtcgagcgcg acaaaggcag ggtctccccc
    25681 ggggggctgc gcctgtacca cagcatgtac cagctgttca gcctcgccat gctggtggtg
    25741 ctcaccacca acaatctcgg cgtgatgtgg gtggcgatgg aggccgccac cctgaccacc
    25801 gtcctgctgg tggcgctgta ccgcacgcac gagtcgctcg aagccgcctg gaaatacttc
    25861 atcctgtgcg gcgtcggcat cgcgcaggcg ctgctcggca ccatcctggt gtacttcgcc
    25921 gccgagaaga tcctcggcgc gggcgtgggc tcgctgctgt ggacgcagct cgacgcggtg
    25981 aagggccagc tggagccgac cgtgatgtcc atcgccttcg tgttcctgct ggtgggctac
    26041 ggcaccaagg tgggcctggt gccgctgcac aactggctgc ccgacgcgca cgccgaggga
    26101 cccacgccga tctcggcggt gctctcgggc ctgctgctca acgtcgcgct gtacgcgctg
    26161 atccgcttca aggtgatcac cgacggcgct ctggagcggc cctttgccag cgccctgctg
    26221 atggggttcg gcctggtgtc ggtggtggtg gcggcattct tcctgtcgcg gcagcgcgac
    26281 gtgaagcgca tgttcgccta ctcctcgatc gagcacatgg gcctgatcac tttcgccttc
    26341 ggcatgggcg gcccggtggc gagcttcgcg gggctgctgc acatgacggt gcactcgctc
    26401 accaagagcg ccatcttctt cgccgtcggc catgccgcgc agcagacggg aacgcaggtg
    26461 atggccgaga tccgcggctt gatccgcgtc aacccgaccg tgggctgggg gctgatgctg
    26521 ggaacggcgg cgatcctcgg catgccgccg ttcggcgtat tcgcgagcga gttcctgatc
    26581 ctcaccaccg ccatcgcgct gcagccctgg gcgacgccga tcctgctcgt gtcgctgggc
    26641 atcgccttcg cggcaatctt cggcaaagtg cagccgatgg tgttcggcga caccagcgcc
    26701 ggcaggctgc cccacccgcc cgcgctggtg ccggtgttca cgcacctcgc gctggtcctg
    26761 tcgctcggac tgtacatgcc gccctacctc gccgactggt accgccaggc tgcgcgcctg
    26821 atcggctgat ccgatggcgc cccagcttcc cgggatttcc ctgacggcgc tgcccggcgc
    26881 cgtacccgcc tggtgcgccg ccgtggacac gcgccagtgg cgctcgggtt gcgaggcagc
    26941 ggtgcgcgcc ggcgcccggc tggcggccct gtgggcgagc gacgagcgcg acgccggcgg
    27001 cggttatgcc gtgcgcgcgc tgtacgtcct gttcgagggg ctggcatgcc tggaactcgc
    27061 gctgccggag cgggacccgc gctaccccga cctgagcgac ctgtaccctt gcgcgggacg
    27121 catgcagcgc gcggccttcg atctggtggg cgtgcgcgcc gcgacccccg agggcgcggc
    27181 gccggacacg cgcggctggt tgcgccatgg cgcgtggcgc gagaccgcgt tcccgctgcg
    27241 ccgcgacgta tcgctggctg cggcgctcga gcccgcggac ccccgttacg ctttcgtcca
    27301 ggtggcgggc gacggggtac acgagattcc ggtcgggccg gtgcacgccg gcaccatcga
    27361 gccggggcac ttccgcttct ccgtcgtcgg cgagcaggtg ctgcgactgg aagaacgcct
    27421 gggctacaag cacaagggca tcgagaagcg cttcgcgtcg ctgaccctgg aggagggcgc
    27481 gcggcttgct gcgcgcgtgt ccggcgacag taccgtcgcc tatggctggg cctacgcgat
    27541 ggcgctcgag ggcctcaccg ggaccaggcc gggcgggcgc gccctgctgc tgcgcgcgct
    27601 gctcctggaa cgcgagcgca tcgccaacca cctgggcgac ctgggcgcgc tgggcaatga
    27661 cggcgggctc gccttcggtc tggcgcagtt ctcgcgcctg aaggaagacc tgctgcgcgc
    27721 caacgcgcgc ctgttcggcc accgctacct gatggaccgc gtggtgcccg gcggtgtcgc
    27781 ctgcgacctg ccgggcgccg cggccgtgga aatactcgag gcgggcgagc gccttgccga
    27841 agagctgcgc agcctgcgcg ccatctacta tgaccattcc ggtctgcagg atcgctgcat
    27901 cacctgcggc cgcctcgcgc cgcgcaccgc cgcactgctc ggcgtggtcg gccttgccgc
    27961 gcgggcgagc ggccacgcca tggacgcgcg cgcgcatccg ggcttcgcgc cctacggcga
    28021 actgcagccc gcggtcgcct cgcaccgcaa cggcgatgtc gcggcacgca tcgtggtgcg
    28081 cttcgaggaa gccttcgaat cgctgcggct gcagcggctc atcctcgagc gcctgcccga
    28141 cgggccgctg cgcgcgccgc tgggcgcggc gcgcgacggc gcgttcggca tcggctgggt
    28201 cgagggctgg cgcggcgagg tgatcgtcgc gctgcagtcg ggcgcgcacg gccgggtgcg
    28261 ccgctgccac ccgcacgacc cgtcgtggca gaattggcca gcgctcgagt atgcggtgat
    28321 cggcaacatc gttcccgatt ttccgctgat caacaagtcc ttcaacctgt cctacgccgg
    28381 cgtggacctc taaccgtgca caagatcctg ttcaagatcg cgcaggtggg cctgaggagc
    28441 gagcccgccc ctgccgcgga cgacacgctg cggcagggcg caccgcgcct caacgaggag
    28501 gtgctgcggc tcttcgggcg ggcgctcgcc atccgccatg tcgacgccgg ctcgtgcaac
    28561 ggctgcgagc tggaaatcaa tgcgctcaac aacccctact acaacctcga ggggcgcggc
    28621 atcaagttcg tcgccagccc gcggcacgcc gacctgctgc tggtcaccgg accggtgtcg
    28681 cgcaacatgg aggaagcgct gctgcgcacc tacgcggcgg taccggaacc caagctggtg
    28741 gtggcggtcg gggactgcgg ttgcgacggc ggcgtgttcg gcgagaacta cgccagccgg
    28801 ggccgcgtgg ccaatgtcat cgcggtggat gtcgcggtgc ccggctgccc gcctccgccg
    28861 gccgccatcc tgcaggcact ggtcaccgcc gtgaccgccc tggcggcggc gaaacgttga
    28921 ctcaggactt gccgccgcaa ctcgcgggca tcacggtaat cggatagccg cggcagttcc
    28981 aggtgacctt gccgccctgc aatgccgggg tgatcgacac ttcgatggcg ttcttttcgt
    29041 tccaaccgcg gatcgcgccg ctcgcttcca cgatccattt cagttcgccg tgcttggggt
    29101 cggtgcgcgc cgccagcttg gcgctggcgc ccgcgccggc cagctggccg gatttctccg
    29161 ccgccgccgc gacctgctgg cgcgccgcct ccgctcccgc gatcagcgcc tgcgcggcct
    29221 ccttcgcgtc gctgcccgcc agctgcggca gcacgaatgc gaccgcgagg aaccccgcca
    29281 gcgccaggcc ggcgatgacc agcaatgcag cacgcatcgt caaatccccc ttttgcagac
    29341 tccgcccgcg cacgcggggt ggagtgacaa ttttccggcg cagcttacca tggacgttca
    29401 tcgcccacag catggctaac gcagagcgcc gcgtctctga caagtgcgga caatccgcct
    29461 gctgatcagc ggccgcgtcc agggtgtcgg ctttcgcgac gccctgcagc gggaggccga
    29521 acggctcagc ctgcgcggtt gggtgcgcaa tcgccgctcc ggcgaggtcg aggcgctggt
    29581 gcagggaccg gcgcaagcgg tggcagccct gcaggattgg gcccgccgcg gtccgcccgc
    29641 cgcccgcgtc gagcggatcg agtgcggcgc gccctgcccc gagcacgcgc gcgactacgc
    29701 gcggttcgag cgctggccga gcgcctgagc gcgctttttc agggcattca ccactgaaaa
    29761 ggcccagggc cggcaggcga tcagcaggcc cacgccctcg cgccgccgcg cgggcagcgt
    29821 cgaatccacc tcggccagct cgttgaactc ggcggccacg cgttccaggc gccgccgcag
    29881 catcgcggcc gaggccggcg agaggtcgcg cgcctcgaag cgcagcagtt ccagcggccc
    29941 gctgaagcgc gagcgcagga actcggtggt cacccgatgc ccgtacgcct tcttcaccgg
    30001 gcctcccgcg cgccacacga agtcccgcgc cacgcgcagg cgcacgcgct cgcccgcgtc
    30061 ccagtcgatc agccgcgcgc gctcgaactt ggcgagcgcc agggtcagct cggtgcggga
    30121 aatggcgaac tcctcgacga tctcgtcgaa cgcccagcgg ttcagcagca gccagaacac
    30181 cgacaacagg cgctcgtcgc tcgccagcag ggtctcctgt tccaggctca gttcggcggc
    30241 ctggtcgtcc gcgccgcgcg ccatgcgcgc aagctcgacc aggtccagct cgagcacccg
    30301 caggatgtcc tcgatgcgcg ccagcgtgaa ggtgccgtgc gagaacaggc gcttcacgct
    30361 ggcctccgac aagcgcaact cgcgcgccag cgcggcgtag gtcatgccgc gcgccctgag
    30421 gcacttcttc agggcgtcga ccaggcgatg ggtcaggggc atgtccgggg gcagatgcga
    30481 ccgccggtat cgtaaaacga gactctgcaa tcggggtatt gcagtgcaac aaacggcacg
    30541 cgaacaatgc gccacccgac gaccccgaga ccgccatgct cgcgacgcaa agcctgcctc
    30601 tccccgccct ccccggaccg cgcgtccatt cggccctgga cctgtgcaag gcagtactcg
    30661 ccggcggcgg cctgcccgac cccctgccga ccagcggcct ggaccgcacg ctgcgccacg
    30721 acgcggcgcg cggactgctc gaggtgcagg ccggcgccac ctggcactct ctcgccgccc
    30781 ataccggcgg cgtggtcctg cccggcaccg tcggcgacag cgtcgccgcc aacagcgccg
    30841 gccccgacgg gcggccggtg gtcgcgcacc tgcgcgccct gaccctggtc acggccgacg
    30901 gcgagctgcg gcgcgccagc cgcgaccggg caccggaact tttccgcctc gcggtcggcg
    30961 gctttggcgc cttcggcccg ttctacagtc tgacgctcga tctcgcgacg ctgtcgcagg
    31021 ccgtcgccga agcgcaagcc ccggtgtgcc tcgggctggc gccggacgag gcgccgggcg
    31081 cgctgcatgg cctggatctg ctggtgccgc cgtccgcggg cgggcagttc gccgcgcagg
    31141 cgcgcgccgc gctcgaggga cgccgttgcg ccctcacccg cctcgaagtg cgccgcgtgc
    31201 tgcgcgagca cgagacgctg ctgtgctggg cgcggcgcga ctatgcggcc gtgcacatcg
    31261 agttccgcac gcgccagacc atcggcgcct gcgtcaacgc ggccgaactg cgccggcagc
    31321 tgatcgcgct ggcgatcgac gccggcggcg cgtgcgcccc gcagcacctg ccgcacgcca
    31381 gccgcgccca ggcggccgcc tgctatccga tgctgggggc gtttctcgcc gagaagcggc
    31441 gctacgatcc cgccgagcgc gtggtcggca cctggtaccg cgccgtgcgc gggctgtggc
    31501 ggcgcgagac ctgcgaggtg cgctggtcgc gcgcctaggc ggcgcccgcg tacacgacct
    31561 gcacgttttc cggcgtcagc cacgcgccca gccgctcgat cagctgcgct tcggggcgca
    31621 cgcgccaggc gtcgccgagc gcgacgtcgc aggccgccgc cccggtacgg tagtgcacga
    31681 acaccgggca ggcgccgtcc gcccggtacg gcgcgagcag ctccatcagc tggcgggcgt
    31741 cggcctggcc gttcatctcc aggcgcaggc gcgcggcata gcgcgcgcgc aggccctgca
    31801 ggtcgtgcag ctcttcggcg atgacgcgca tgccgccggc aaactcgtcg cgctgcacct
    31861 tgccctgcac caccagcagc gaatcctcct tcagcttgtc gcggtgccgc tcgaacagct
    31921 cgctgaacac cgtgacctcg acctgcgccg tggcgtcatc gagcagcacc gccatcatgc
    31981 ggccgcggcg cgtcatctgc gaacgcgccg cgttcaccac gccggcgagc cacacgcgct
    32041 gctcgcccgc cgacagccgc gccagcggcg tgcgcgggaa gccggcgatc tcgcgcgcgt
    32101 acaccgagaa caggtgcccg gagagggcaa agcccagcgc cgccttctcc tcgagcagca
    32161 ggcgccgcag gtcccaggcc ggcgtctcga ccagcggccc cgccgcgccc cgcgcagact
    32221 cggcgtcgcc gaacaggctc acctgcgccg cctggcgctc ggcctgctcg gccgcctcca
    32281 gcgcgcgccc gacgctcgcc agcaggctgg cgcgattctt ctgcagcgtg tcgaaggcgc
    32341 cagcgcgcac cagcgcttcg accgcgcggc ggttgacgat gcgcttgtcc acgcgccggc
    32401 acaggtcgaa caggtccgtg tacggaccgg accggcgcgc ctgcaggatc gcgccgaccg
    32461 ccgcttcgcc ggtgccgcgc accgcgccga gaccgtagcg gatggttttc gcgtccaccg
    32521 gccggaagcg gtagtccgag gcattgacgt ccggcggcag caccgtgagg ccgttggcga
    32581 gcgcatcctc gtggaactgg cgcaccttgt ccgtgtcgtc catcaccagt gacaggttgg
    32641 cggccatgaa cgccgcgcca tggtgcgcct tcatgtaggc ggtctggtag gcgagcagcg
    32701 cgtaggccgc ggcgtgcgag cggttgaagc cgtagccggc gaacttctcc atcaggtcga
    32761 acagctgggt cgccctgccc cgcgacagcc cgttcttctc cgcgccggcg acgaacacgt
    32821 cgcgctgctg cgccatctcc tcgggcttct tcttgcccat cgcccggcgc agcaggtcgg
    32881 cgccgcccag cgtcaggccg ccgatcacct ggccgatctg catcacctgt tcctggtaga
    32941 ccatgatgcc gtaggtcggg ctgaggatcg gctcgaggcg cttgtcgagg tactcgacgc
    33001 gctggctgcc gtgcttgcgc gcgatgaagt ccgggatcag gtccatcggg ccgggccggt
    33061 acagcgccac cagcgcgatc acgtcctcga agcggtccgg gcgcgcgcgc ttgatcaggt
    33121 cgcgcatgcc gcgcgattcc agctggaaca ccgcggtggt attgcccgcg gacagcagct
    33181 ggtaggtcgc cgcgtcgtcc agcggcacgc gctccagcga aaactcggcc gcgcccagcg
    33241 cgcgcacgta gcgctccgcc cagtcgagga tggtgagcgt ggtcaggccg aggaagtcga
    33301 acttcaccag cccgaccgcc tcgacgtcgt ccttgtcgag ctgcgagatg acgttgctgg
    33361 tgccctcggc ggcgtacagc gggcagaaat cggtcagccg gcccggcgcg atcagcacgc
    33421 cgccggcgtg catgccgacg ttgcgcgtca gcccctcgag cttctcgccc agggcgagca
    33481 gctccttcac ctcgtcctcg ttcttctcgc gctgcgcgag cagcggctcc atttcgcgcg
    33541 cgtcctgcag cgtgatcagg cggccgggct ggaacgggat cagcttggcg atctggtcgc
    33601 agaagttgta gcccaggtcg agcacgcgcc cgacgtcgcg caccaccgcg cgcgccgcca
    33661 tggtgccgaa ggtgacgatc tgcgacacgc agtcctcgcc gtacttgcgc cgcacatagt
    33721 cgatcacctt gtcgcgcccg tcctggcaga agtcgatgtc aaagtccggc atcgacacgc
    33781 gctccgggtt gaggaagcgc tcgaacagca ggtcgtaagt gatcgggtcg aggtcggtga
    33841 tgcccagcga gtacgccacc agcgagccgg cgcccgagcc gcgcccggga ccgaccggca
    33901 cgccgttgtg gcgcgcccag ctgatgaagt cggcgacgat gaggaagtat ccggcgaagc
    33961 ccatctggat gatggtgcgg atctcgaagt ccaggcgctg gcgatagcgc ggctccgcgg
    34021 cggcgcgcgc gccgtccgcg ggaaacagct tcgccagccg cgccgccagc cccgcctcgg
    34081 cctgctggcg caggacctgg tcgaccgtgg cgcccggcgg cgtcgggaac gcgggcaggc
    34141 ggctcctgcc gagctcgatc tccaggctgc agcgccgcgc gatctccagc gcgttgtcga
    34201 gcgcctgcgg cacgtcggcg aacagctgcg ccatttccgc ctgcgacttg aagtactgct
    34261 cgggggtgaa cagcttcggc cggcgctggt cgccgagcac gtagccctgc gcaatgcaca
    34321 cgcgcgcctc gtgcgccttg aaatcgtcgg ccgcgaggaa ctgcaccggg tgcgtggcga
    34381 ccaccggcag gccgaggcgc gcggcgagct ccagtgagcg cgcgagcagc acttcgccat
    34441 gcgcaaatcc ggcgcgctgc acctccaggt aatagcggcc cgggaacagc gccgcccact
    34501 cgcgtgccgc cttctccgcc gccgccgcgc cgcccgcggc cagcgcctgc ccgacgtcgc
    34561 cgccggccgc gccggagagc gcgatcaggc cctcgctgcc gccttcgcgc agccaggagc
    34621 gcgcgatctc cgcgcgcgcg cggtgctggt tcttcagcca ggcgcgcgac agcagctcgc
    34681 acaggcgcag gtagcccgcg cgcgacgcgc acagcaacag caggcgcgac ggcttgtcgc
    34741 ccgcggcctc gccctgcacc cagcagtcgg cgccgatcac cggcttgacg ccggcagcgc
    34801 gcgcggcgcg gtagaacttc accatgccga acaggttggc gctgtcggtg agcgccagcg
    34861 ccggcatgcc gtccccggcc gcgcgctgca ccgccgcgtc gagccggacg atcccgtccg
    34921 tcaccgaata ctcgctgtgc agcctgagat ggacgaagcg aggagcagcc atgggggacg
    34981 gcgaaagttt accatccgcg catgcgcgcc tacgccctcg tcgccctgtt cgccggcgcg
    35041 acttgcatcg ccttgtcgcc gatctgggtg cgcgtggcgg acgtcgggcc gacggcgagc
    35101 gcgttctggc gcgtcgcgct cgccgcgccg ctgctgtggg gcgcgctggc gttcactgcg
    35161 cgcgccggcg ccgcgcggcc gcactggccg ctgctcgccg cggccggatt tgcattcgcc
    35221 ggcgacctcg ccttctggca ctggtcgatt cagaccacat ccgtcgccaa cgcgacgctg
    35281 ctcgccaacc tctcctcgat cttcgtcacg ctggccgcct ggctgctgtg gcgccagcgc
    35341 cccagcgcgc agttcgtcgt cggcctggcg ctggcgctcg tcggcgtggc gctgctcgtg
    35401 cgcacgagcc tcggcgtgtc cggcaccgcg ctgctcggcg acgggctggg ggtgatcacg
    35461 gcggtgttct atgcctggta cctgctgacg gtgaagcgcc tgcgcgacct gggcgccgcg
    35521 acgctgcgac tgatggccgt caccaccacg ctcaccgcgg tgatcctgtt tcccgtggcg
    35581 ctggcctccg gcgaggcgct gcttccggcg ggcgcccggg gctggctggt actgctcggg
    35641 ctcgcctgga tcacgcacgc cggcgggcag ggcctgatca cctattcgct cgcgcacctc
    35701 cccgcggcgt tctcctcggt gggcctgctg ctgcagccgg tgatggcggc ggcttttgcc
    35761 tggaccctgc tcggtgagcc gctggccgcg ccgcaggttg ccggcggcgc ggtggtgctg
    35821 gcggcgatct accttgcgcg ccgcggctcg aaagggtcag aataacccgt cgcgtcaggc
    35881 ggcatcggcc gccaggaggg tcccatggcg gtgaagaaga agctcaaggc gaaggccagg
    35941 tcgagcagca agaccaagcg caggaaaatc gccaagccgg cggcaaaggc cgtccggaaa
    36001 ccggcacgca aggcgcctgc ccggcgcgca gcgccgaaaa aggctgccgg gccgaagctt
    36061 gccaaaccca gggtcgccaa ggcgccgcct gcaatcccgg tcgagcggcc ggcccccggc
    36121 gagcgcatcg gcgtggtcac ccactactac ggcaacccga tggtggcgat cgtgaagctg
    36181 gagacgggca ccctgcgcgt cggcgacaac atccacatcc gcggccacac cagcgacttc
    36241 agccagcggg tggagtccat ccaggtcgat cacgcggcgg tgaacgaagc cggtccgcag
    36301 gacgatttcg gcatcaaggt gatcgagcac gcgcgcgagc acgacgtggt ctacaagctg
    36361 ccccgctagc ggatcaccgc ctcgggcgcg cctttggctt tttccacgct gccgatctcg
    36421 tagaccgtct cccccgccgc gcgcagcctg gcggccgcgc gcttggcgtc ggccgccgcc
    36481 accaccgcga ccatgccgat gccgcaattg aacacgcggt acatctcgtc ctccgcgacg
    36541 ccgccctgct gctgcagcca gcggaacagc gccggacgcg gccagctgtt cttgcggatc
    36601 acggcccgcg tgccgccggg cagcgtgcgc ggcacgttgc cggtcagccc gccgccggta
    36661 atgtgcgcca gccccttgac tcgcaccgtg cgcaacagct tgagcaccgg cttcacgtag
    36721 atgcgcgtcg gttccatcaa ggccgcgagc agcgccgcgt ccggccgcgc gcgttcgacg
    36781 attttgcgga tcagcgagta gccgttggaa tgcgcgccgc tcgaggcgag gccgagcagg
    36841 atgtcgcccg gcctgatcga gcggccatgg atgatcttgc tcctctccac cacgccgacg
    36901 caaaagccgg ccaggtcgta ctcgcccggc gggtacatgc cgggcatctc cgcggtctcc
    36961 ccgccgatca gcgcgcagcc ggcaatctcg cagccgcgcg cgatgccctt caccacctgc
    37021 gcggccaccg cggcgtgcag cttgccgcag gcgtagtagt cgaggaagaa caacggctcc
    37081 gcgccctgca ccaggacgtc gttcacgctc atcgccacca ggtcgatgcc cacggtgtcg
    37141 tggcgctcga gcgcgaaggc cagcttcagc ttggtgccga cgccgtcggt gcccgacacc
    37201 agcaccggct ggcggtattt cttcgggatg cggcacagcg cgccgaagcc gccgatgcct
    37261 gccagcacct cggggcgcat ggtgcgccgg gcgtagggct tgatggcctc gacgagggca
    37321 tcgccggcgt cgatgtcgac gccggcgtcg cggtaggaaa gcggatggct catgctagag
    37381 tttgccgttt gcagcgcgat tttacttgag atgcccaccc cgttctccgc caatccgcag
    37441 ttctggggct ggttcgccat tgcgctggtg gccggcggcc tggtgtatct cctgtcgccg
    37501 atcctcgcgc cgttcctgtt cgcggcgatc ctcgcgtaca tcctcgatcc ggtcgtcgag
    37561 cgcatcaccg gtaaatacct gccgcgcacg ctggcggtat tgctggtgat gctcgcgctg
    37621 ctcgcgctgc tggcggcctt cgccctcgtc gtgctgccgc tgttcacgaa ggagctgcgc
    37681 cagctggccg agcgcctgcc cgcgttcatc ggctggctca atcagcattt cgcgccgttc
    37741 ctggagcgtc agctcggcgt cgaagtgcgc ttcgacaccg acacggtgcg ccgcgtcgcc
    37801 gccgagctgg tggccgacaa cgaggacctg gtggggcggc tgctcggtac gctcaagatc
    37861 ggcggcctcg cggtgctcgc attcctcgtc aacctgctgc tggtgccggt ggtgctgttc
    37921 tacctgctgc gcgactggca cgccctgcgc gcgcgcgtcg accgcctgat cccgcgccac
    37981 gcgcatgcca aggcgctgga gatcctgcgc gaggtggacg cggtgctggc agagttcctg
    38041 cgcgggcagc tgctggtcat cgcggcgatg agcgcgttct acgtgatcgc gctgtggctg
    38101 gcgcggctgg agttcgccct gccgatcggc ctgatcaccg gcgcgctggt gttcattccc
    38161 tacgtcggcg ccatgctcgg cttcgtgctg gcgagcatcg ccgcgctgct gcagttcgac
    38221 agcctgtggg gcctcgcctg ggtatggctt gccttcggcg tcggccagtc cctggaaggc
    38281 atggtggtga cgccctggct ggtgggcgag cgcatcggcc tgcacccggt ggcggtgatc
    38341 ttcgcgctgc tcgccttcgg ccagctgttc ggcttcttcg gcgtgctgct tgccctgccc
    38401 gcgagcgcgg cgctgctcgt cgccctgcgc cacctgcgcc gcgcctacct cgcgagctcg
    38461 ctgtacggcg gcgcgcagtg acgcgccagc tcgcgctgcc catctcgccg ccgccgcagc
    38521 ccacgctcga caacttcgtc cccggcgcca atgccgaact gctggcgcgg ctgcgccagc
    38581 tggcggcggg agagctcgcc gagtcggtgc tgtacctgtg gggcgagccg ggcagcggcc
    38641 gcagccacct gctcgccgcg agcgcgcgcc ccggcctcac gcttgccgac gacgtcgagc
    38701 ggctggacga gccgcggcaa atcgcgctgt tcaacgccat caacgccgcg cgcgatgcgg
    38761 gcgcgacggt gctcgccgcc ggcgacgccc cgcccgcgca gctggcgctg cgcgcggacc
    38821 tgaagagccg gctcgcctgg ggcctggtgt accaggtgaa gccgctgagc gacgcggaga
    38881 aggcgctcgc gctgcacgcc gaggcggcgc gccgcggcct gaagctctcc gacgaggtgg
    38941 tctggtatct gctcacccac gtgcgccgcg acctgcactc gctcagcgcg ctgctggcgc
    39001 agatcgaccg cgcctcgctc gagcagcggc gcgccgtcac gctgccgctg gtgcgcgagc
    39061 tgatccgggg cctggacgag tgacgctcgg cctgttcgac ctggacaaca cgctgctcgc
    39121 gggcgacagc gactacgagt ggggccagtt cctggtggac cgcggcgtcc tgccgcgcgc
    39181 tgaatacgag gcgcagaacg cggccttttt cggccagtac aaggccggga ccctggatat
    39241 ccacgagtac ctcggcttcg cgctgcgtcc gctcgccgcg cacacgccgc aggacctggc
    39301 gcgctggcgc gcggaattca tgcgcgaacg catcctgccg atgatcacgc cggcggcgcg
    39361 cgccctcgtg cgccggcacc tggaggcggg agagctgtgc gccatcgtca ccgccaccaa
    39421 cagctttgtc accgcgccga tcgcgcgcga gttcggcgtg gcgcatctcg tcgccaccga
    39481 gcccgagacg gcgcagggcc gcttcaccgg gcgcgtggcc ggcacgccct gcttccgcga
    39541 aggcaagatc cggcgcgtgg acgaatggct cgccggcctc gggcgccgcc tggaggactt
    39601 cgccgagagc cgcttctaca gcgattcgca caacgacctg ccgctgctcg agcgcgtgcg
    39661 ccgcccggtg gccgtggacc ccgacccgca gctcgccgcc gaggcggcgc gccgcggctg
    39721 gccggtgatg tcgctgcgct agaatcgccg cctcgatgat caagcgcttc atccgccgcg
    39781 tactgggact gcctgcgggc gcgccgcagc gcgtgccgcg cgagcgccac ggcctgcggc
    39841 gcgaggacct cagccagaac gcgctcaccg tgtgcgacgc gctgcgcgcg gcggggtact
    39901 ccgccagcgt ggtgggcggc gcggtgcgcg acctgctgct gcgcatcgag cccaaggact
    39961 acgacgtcgc caccgacgcg cgccccgagc aggtgcggcc gctgttccgg cgcgcgctga
    40021 tcatcgggcg gcgcttccgc ctggtgcacg tgatgctggg ccaggacacc gtggaggtgt
    40081 cgactttccg cgccgccgac gccaacaccg ccgagaagga cgagcacggc cgggtgctgc
    40141 gcgacaacgt gttcggcacc caggaggaag acgcgcggcg gcgcgacttc accatcaacg
    40201 cgctgtactt cgacccggcg accgaggaga tcatcgacta ccacggcggc ctcgccgacc
    40261 tgaagaagcg cgtgctgcgc atgatcggcg acccggtgac gcgctttcgc gaagaccccg
    40321 tgcgcatgct gcgcgccgtg cgcctggccg ccaagctcgg cttgagcatc gaggcgggca
    40381 cgcgcgcgcc gatccgccgc ctcgcgccgc tcgtcgagca cgtgccgccg gcgcgcgtgt
    40441 tcgacgagat gctcaagctc ctcatgtccg ggcacgccag cgcctgcgtg cgccagctgc
    40501 gcgaggaagg cctggcgaag ggcgtgctgc cgctgctcga cgtgatcctc gagcagcccc
    40561 tgggcgagcg cttcgtcacc ctggcgctgg cgcagaccga cgagcgcgtg cgcaccgagc
    40621 ggccggtatc gccggcgttc ctgttcgcgg cgctgctttg gcacgaggtg ctggcggcat
    40681 tgaagtcgcg ccaggcgaag ggagagcggg cgcagcccgc gctggaggcc gcgatggacc
    40741 gggtgctcga tgcgcagtgc gagaagctgg cgctgacgcg caagctcacc gcgaccatgc
    40801 gcgaggtgtg ggccctgcag cccaggttcg agcagcgcgg cggccagcgc ccctaccgcc
    40861 tggtcgagtc gccgcgcttc cgcatggcct acgatttcct cgccctgcgc gcggcgagcg
    40921 gcgaagtcag cccggaactg gaggcctggt ggcgcgcctt ccagtccgcc ggcgcggaga
    40981 cccgccaggc gatgctcctg cccgataccg ggccgcgcaa aaagcgccgc cgccgccgcc
    41041 ggcgcaagga aggcgcggag ccgggcccgg caccggcgtg accgtcgcct acgtcggcct
    41101 cggctccaac ctgggcgatg cgcgcgccca tgtgctgcgc gcattcgagg atctggcgcg
    41161 gctgccggag acgcgcctcg aggcgcgctc ggcgctgtac cgcagcgcgc cgctggacgc
    41221 cccggggcag gcggactacg tcaacgcggc ggcgggcgtg gatacggaac tgtcggccgc
    41281 gcagctgctc gccgcgctgc aggacatcga gcggcgccac ggccgcgagc ggccctacgc
    41341 caacgcgccg cgcacgctcg acctcgacct cctgctctac gacgaggcgg ccttcgaatc
    41401 ggcgatgctg accctgccgc acccgcgcat gcaccagcgc gccttcgtcc tcgcgccgct
    41461 cgcggagctc gatccgcgcc tcgaggtgcc gggccgcggc agcgtgcgcg agctgctggc
    41521 gcgctgcgcc ggccagcgcg tcgcgcgcct ggactgagat ggagcgcctg cgctatatcg
    41581 cggtggaggg cccgatcggc gccggcaaga ccagcctggc gcggcggctc gccgcgcgcc
    41641 tcggcgccga cctgctgctc gagcagcccg gggaaaaccc gttcctcgcg cgtttctacc
    41701 gcgacatggc gcgctacgcg ctgccgacgc agctgttctt cctcttccag cgctcgcgca
    41761 tgctcgagcc gctcgcccag cccgacatgt tcggccgctc catcgtcggc gactttctcc
    41821 tcgacaagga cccgctgttc gcgcgcctca cgctgtcggc cgacgagctc gcgctctacc
    41881 agaagatcta cgacgcgctg cgcccgcgcg cgcccacgcc cgacctcgtc atctacctgc
    41941 aggcgccctc cgccaccctc atcgagcgcg tgcgccggcg cgcggcggcg ttcgagcgcg
    42001 gcatcgacga ggagtacctg gcgctgctgg ccgagagcta cgcgcgcttc ttctacca
//
LOCUS       DASRIE010000240         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_776340, whole genome shotgun sequence.
ACCESSION   DASRIE010000240 DASRIE010000000
VERSION     DASRIE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776340"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFB53_10465"
     CDS             <1..196
                     /locus_tag="VFB53_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87192.1"
                     /translation="AVAYCWTQALQYFPEGERARMERWFYGEHFAELMRWDERYRKNL
                     VRAHTSFPVSFSMTARKPAR"
     gene            201..911
                     /locus_tag="VFB53_10470"
     CDS             201..911
                     /locus_tag="VFB53_10470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HZN87193.1"
                     /translation="MAYSGHAVLAVVPARGGSKGIPRKNLAKVGGKSLIERAALVVKA
                     LPWLDAAVLSTDDDEIAAEGRRCGLAVPFRRPAALAADNAGALEMWRHAWLECERHYG
                     RKFELSVLLEPTSPLRQAQDVEKTLAALVSGNHAAAATVSRTPAHYAADKLLTLKSGV
                     IAFALAAGAQIRQRQQLPAYYHRNGACYAVRRATLVDEGTIIERDCAAVVIERALVNV
                     DEPFDLELAEHLARRDAL"
     gene            919..1770
                     /locus_tag="VFB53_10475"
     CDS             919..1770
                     /locus_tag="VFB53_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZN87194.1"
                     /translation="MGLRELVARSRVLVEDMVLTSPRLAFRPRLAEGMPVSEYVRGLQ
                     DKGYASIGSLLSPAELREIRTQIDAALSEKRADIAVKWSDKVQYARVINPLRLHEKLL
                     ALAAHPLALAVAEAYFRRQPYLADVDLRRVEAVSMESLGPQHVSSSSWHRDTRGRQLK
                     LMVYLSDVTAKDSNFSLVPGSHRGQHLRKGTYMESRLGDAEVADLQTLEWYGAAGEAM
                     LFDTNVIHRLRRKAGAAVRDSITYYYTPGQSLFALDYPAEALHHLPEASRVVFSEPGW
                     PLSRLAA"
     gene            1743..>2445
                     /locus_tag="VFB53_10480"
     CDS             1743..>2445
                     /locus_tag="VFB53_10480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN87195.1"
                     /translation="MAAVAPRGVVFPVQRSDIEHALEPYVLRRFELGKSAGSLWWARL
                     RALLGRRAKSVPRVRHDYSGVWSQDQLPGAPAVENARYLLRWGEQGIEVRGWAEKRVH
                     LLLLSRALDALRPASVLEVGSGNGLVLMMLATGHAQVRFSGVELTEAGLQAARRMQSQ
                     PSLPPAVLNSLPYPASSPEAFRAVEFQQGNAAQLPFADKSVDLAMTSLALEQMNEVRR
                     AAFRELARVARKWVLM"
BASE COUNT          365 a          849 c          858 g          373 t
ORIGIN      
        1 ggcggtggcg tactgctgga cgcaggcgct gcaatacttc ccggagggcg agcgcgcgcg
       61 catggagcgc tggttctacg gcgagcactt cgccgagctg atgcgctggg acgagcgcta
      121 ccgcaagaac ctggtgcgcg cacacaccag ttttccggtg agcttctcga tgaccgcgcg
      181 caagccggcg cgctagggac atggcgtatt ccgggcacgc cgtcctcgcc gtggtgccgg
      241 cgcgcggcgg cagcaagggc atcccgcgca agaacctggc caaggtgggc ggcaagtcgc
      301 tgatcgagcg cgccgcgctg gtggtcaagg cgctgccttg gctagatgcc gcggtactct
      361 ccaccgacga cgacgagatc gcggccgaag ggcgccgctg cggcctcgcg gtgccgttcc
      421 gccgccccgc cgcgctggct gccgacaacg ccggagcgct cgagatgtgg cgccacgcct
      481 ggctcgagtg cgagcggcac tatggccgga agttcgagct ctccgtgctg ctcgagccga
      541 ccagcccgct gcgccaggcg caggacgtgg aaaagaccct cgcggcgctc gtgtccggaa
      601 accacgccgc cgcggcgacc gtcagtcgca cgcccgcgca ttacgcggcg gataagctcc
      661 tgacattgaa aagcggagtc attgcttttg cgcttgcggc cggcgcgcag atccgccagc
      721 gccaacagct gccagcctac taccaccgca acggcgcctg ctacgcggtg cgccgcgcca
      781 cgctggtcga cgaaggaacg atcatcgagc gcgactgcgc cgcggtggtg atcgagcggg
      841 cgctcgtgaa cgtcgacgag ccgttcgacc tcgagctcgc tgagcatctc gcgcgccgcg
      901 acgccctata atcccgctat gggcttgcgc gaactggtgg cgcgatcgcg ggtgctggtt
      961 gaggacatgg tcctcacctc gccgcggctc gcgttccgcc cgcgcctcgc tgagggcatg
     1021 ccggtcagcg agtacgtgcg gggcctgcag gacaaaggct atgcgtcgat cggctcgctg
     1081 ctctcgcccg ccgagctgcg cgagatccgc acgcaaatcg acgccgcgct gtcggaaaag
     1141 cgcgccgaca tcgcggtgaa gtggagcgac aaggttcagt acgcgcgggt gatcaacccg
     1201 ctgcgcctgc acgagaagct cctcgcgctc gcggcgcatc cgctggcgct cgcggtcgcg
     1261 gaagcttact tccgccgcca gccctacctc gccgacgtcg acctgcggcg cgtcgaggca
     1321 gtgtcgatgg agagcctcgg gccgcagcac gtctcgtcga gcagctggca ccgcgatacg
     1381 cgcggccggc agctcaagct gatggtgtac ctgagcgacg tcaccgccaa ggacagcaac
     1441 ttctcgctgg tgcccggctc gcaccgaggc cagcacctgc gcaagggcac ctatatggag
     1501 agccgcctgg gcgacgcgga ggtcgccgac ctgcagacgc tcgagtggta cggcgcggcg
     1561 ggcgaggcga tgctgttcga caccaacgtg atccaccggc tgcggcgcaa ggccggcgcc
     1621 gcggtgcgcg actcgatcac ctattactac acgccggggc agtcgctgtt cgcgctcgac
     1681 taccccgcgg aagccctgca ccaccttccc gaggcgagcc gcgtggtgtt cagcgagccc
     1741 ggatggccgc tgtcgcgcct cgcggcgtag tgttcccggt ccagcgcagc gacatcgagc
     1801 acgccctcga gccgtacgtg ctgcgccgct tcgagttggg caaaagcgcc ggcagcttgt
     1861 ggtgggcgcg gctgcgcgcc ctcctcgggc gccgcgcgaa aagcgtgccg cgcgtgcgcc
     1921 acgactattc gggcgtctgg tctcaggacc agctgccggg cgcgccggcg gtggagaatg
     1981 cgcgctatct gttgcgctgg ggcgagcagg gcatcgaggt gcgcgggtgg gcggagaagc
     2041 gcgtccacct gctgctgctg tcgcgcgcgc tagatgcgct gcggccggcg agcgtgctcg
     2101 aagtcggcag cggcaacggc ctggtcctga tgatgctcgc taccgggcac gcgcaagtcc
     2161 gcttctcggg tgtcgagctc accgaggccg gcttgcaggc cgcgcgccgc atgcaatcgc
     2221 agccctcgct cccccccgcc gtgttgaact cgttgccgta tcctgcgtcg tcccccgagg
     2281 ctttccgtgc ggttgaattc cagcagggaa atgccgccca gcttccgttc gccgacaaga
     2341 gcgtcgatct cgcgatgacc agcctcgcgc tcgagcagat gaacgaggta cgccgcgccg
     2401 cgttccgcga gctcgcgcgc gtggcgcgta agtgggtgct gatgc
//
LOCUS       DASRIE010000241         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_481974, whole genome shotgun sequence.
ACCESSION   DASRIE010000241 DASRIE010000000
VERSION     DASRIE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481974"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFB53_10485"
     CDS             <1..1011
                     /locus_tag="VFB53_10485"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZN87196.1"
                     /translation="SPAVLAHVHHPFPATLRTELLALNSPGHDELARAAACGNELARR
                     YAAAVGDALARAAVAASEVRAIGCHGQTVRHRPDAGYTVQLGNTALLAELAGARVVAD
                     FRSRDVAAGGQGAPLAPAFHAAVFGDRGEDRAVLNLGGIANLSCLPVAGDILGFDSGP
                     GNCLMDLWTQSQRGQPFDEDGAWAAGARHDAALLAGLLAEPYFALPPPKSTGRDLFNE
                     GWLRARLPGTRDARQVQATLLELTARSIADALRRCCPGARRLIVCGGGARNRRLMDRL
                     GELLAPLAVEASERYGIAPSLVEAVAFAWLARRTLQELPGNLPSVTGARGARVLGAVY
                     PP"
     gene            complement(1019..1369)
                     /gene="erpA"
                     /locus_tag="VFB53_10490"
     CDS             complement(1019..1369)
                     /gene="erpA"
                     /locus_tag="VFB53_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZN87197.1"
                     /translation="MNAVTEMPAPLVFSDNAAGKVKQLIDEEGNAELKLRVFVSGGGC
                     SGFQYGFTFDEVQNEDDTVMQKNGVTLLIDPMSFQYLAGAEIDYQEGLEGAQFVIKNP
                     NATTTCGCGSSFSA"
     gene            complement(1392..1805)
                     /locus_tag="VFB53_10495"
     CDS             complement(1392..1805)
                     /locus_tag="VFB53_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZN87198.1"
                     /translation="MFSKSAKPQSRIDSLIGATTRIEGNVFFSGGLRVDGAVRGNVAA
                     LPDQPGTLVVSEQARIDGEVSASHIVVNGTINGPVHAAETLELQVGSRVKGDVHYKNL
                     EIQQGAIVEGRLVHQGGVEIKGVELKLASGGDKPR"
     gene            complement(1821..2585)
                     /locus_tag="VFB53_10500"
     CDS             complement(1821..2585)
                     /locus_tag="VFB53_10500"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZN87199.1"
                     /translation="MRLSSKVLKLRQRFGIAAPRVAVRSEVAWYLRWIGLILVLACSA
                     ALAAWMYDAGRRFAGFDRSEVEQALADARRDLEATRSELERVRAVANAADSRLSIERT
                     AQQKLVQQTRALEQETARLREELAIFESMLSAEQRAANPLSINRFKVVPDVLPGEYRY
                     RLLLLLAPGSRRDRDFQGRLELVVGLTEGGRNAMMTFPESGDPSASAFRLTFRHFHRV
                     EGIFRVDPKAKVESVQVRVYEGTSDQVRAVQSAAPG"
BASE COUNT          391 a          973 c          925 g          427 t
ORIGIN      
        1 tccccggcag tgctcgccca tgtccatcat ccttttcccg cgaccctgcg caccgagctc
       61 ctggcgctga acagcccggg gcacgacgag ctcgcgcggg ccgccgcctg cggcaatgag
      121 ctcgcgcgcc gctacgccgc agcggtcggc gacgcgctcg cgcgcgcggc ggtggcggcg
      181 agcgaagtgc gcgccatcgg ctgccacggc cagaccgtgc gtcaccggcc cgacgccggc
      241 tacacggtgc agctgggcaa cacggcgctg ctcgcggagc ttgcgggcgc gcgggtggtg
      301 gcggatttcc gcagccgcga cgttgccgcg ggcggccagg gcgcgccgtt ggcgcccgcg
      361 ttccacgccg cggtcttcgg cgaccgcggc gaggatcgcg cggtgctgaa tctcggcggc
      421 atcgccaacc taagctgctt gccggttgcg ggggacatcc tcggattcga cagcgggccg
      481 ggaaactgcc tgatggacct gtggacgcag tcgcagcgcg gccagccgtt cgatgaggac
      541 ggcgcctggg cggcaggggc gcggcatgac gccgcactgc tcgccggcct gctcgccgag
      601 ccctacttcg ccctgccgcc gccgaaaagc accgggcgcg acctgttcaa cgagggctgg
      661 ctgcgtgcgc ggctgcccgg cacgcgcgac gcgcggcagg tgcaggcgac actgctggaa
      721 ttgaccgcgc gcagcattgc cgatgcgctg cgccgctgct gtcccggcgc gcgccgcctc
      781 atcgtctgcg ggggcggggc gcgcaatcgc agactgatgg acaggttggg ggaacttctt
      841 gcgccgcttg cggtggaggc cagcgaacgc tacggcattg cgccgtcgct ggtcgaggcg
      901 gtggcattcg cctggctcgc gcgacgcacc ctgcaggaac tgcccggcaa cctgccttcg
      961 gtcaccggtg cgcgcggcgc gcgggtgctc ggcgcggtct acccgccgta gataacggtc
     1021 aggccgagaa cgacgacccg caaccgcagg tggtggtggc gttcgggttc ttgatcacga
     1081 actgcgcgcc ttccagccct tcctggtagt cgatctcggc gccggcgagg tactggaagc
     1141 tcatcgggtc gatgagcagc gtgacgccgt tcttctgcat cacggtgtcg tcctcgttct
     1201 gcacctcgtc gaaggtgaag ccgtactgga agcccgagca gccgccgcca gagacgaaca
     1261 cgcgcagctt gagctccgca ttgccttcct cgtcgatcag ctgcttgacc ttgccggcgg
     1321 cattgtcgga gaagaccagc ggtgccggca tttcggtgac tgcattcatg tgcgctccat
     1381 tgccagatat ttcaacgcgg cttgtcgcca cccgaggcga gcttcaactc gacgcccttt
     1441 atctcgaccc cgccctgatg caccaggcgt ccctcgacga tcgctccctg ctggatctcg
     1501 aggttcttgt aatgcacatc ccctttgacg cgcgagccga cctggagttc aagtgtctcc
     1561 gcggcatgca ccgggccgtt gatggtgccg ttgaccacga tatgcgaggc cgacacctcg
     1621 ccgtcgatgc gcgcctgctc cgacaccacc agcgtgcccg gctggtcggg gagcgccgcg
     1681 acgttgccgc gcaccgcgcc gtccacgcgc aggccgccgc tgaagaacac gttgccctcg
     1741 atgcgcgtgg tggcgccgat caggctgtcg atccggctct gcggcttcgc gctcttgctg
     1801 aacatgatgc ggtacctcct ttagcccggc gcggcgctct gcactgcgcg cacctggtcc
     1861 gaagtcccct cgtaaacgcg cacctgcacg ctttcgacct tggctttcgg gtccacgcgg
     1921 aaaatcccct cgacgcgatg gaagtgcctg aaagtcaacc ggaaagcgga ggcgctgggg
     1981 tccccgcttt cggggaaggt catcatagca tttctgcccc cctccgtcag gccgaccacc
     2041 agctcgagcc gcccctggaa gtcgcggtcg cgccggctcc ccggcgcaag cagcagcagc
     2101 agccggtaac ggtactcccc cggcaggacg tcgggcacca ccttgaagcg gttgatcgac
     2161 agcgggttgg ccgcgcgctg ctcggcggag agcatgctct cgaagatcgc cagctcctcg
     2221 cgcaggcgcg cggtctcctg ctccagggcg cgcgtctgct gcaccagctt ctgctgcgcg
     2281 gtgcgctcga tcgacagccg gctgtccgcg gcattggcca ccgcgcgcac ccgctccagc
     2341 tcgctgcgcg tggcctccag gtcgcgacgc gcgtcggcca gcgcctgctc cacctcgctg
     2401 cggtcgaagc cggcgaagcg gcgccccgcg tcgtacatcc aggccgcgag cgccgcggag
     2461 caggccagca ccaggatcag cccgatccag cgcaggtacc acgccacctc ggaacgcacc
     2521 gccacgcgcg gcgcggcgat gccgaagcgc tgccgaagtt tgaggacctt ggaagaaagc
     2581 cgcacggcgc gatgatgcat ccgctcaggg gaaaaaacaa aaggccgccc cgggggcggc
     2641 ctcgcgcagg cagcgcgtcg tgccggcgct cagcgcttgg agaactgcgg gcggcggcgc
     2701 gccttgcgca gtccga
//
LOCUS       DASRIE010000242         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3350302, whole genome shotgun sequence.
ACCESSION   DASRIE010000242 DASRIE010000000
VERSION     DASRIE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3350302"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VFB53_10505"
     CDS             <1..1168
                     /locus_tag="VFB53_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87200.1"
                     /translation="VIGLFLLCAALLQRWLPRGSLRPLVASTLLAVGAVWFRVQVFAV
                     GFPAWLAAAALAVPAVRARKLLSAGAALLAFLLFVIAFYAATDAVFALPVFLEVLHDR
                     QEPTAYTGLYLEMRDTPYGIPAGIVLVYAAALGALVVLYPLALWLAHRARALRAGDSF
                     PGLVLAAYLAVMLTAPIDKFRDSTEFTVRPFVLAYAAVAVWTVCLLCRVFALHWPQRA
                     PQAWRTMFGACLVGVALAWHGTPAMGLAKFEWAALFYPKRIDAGLTQAGRYLREHGRP
                     GQVFAVRGLQLGWAATDAAVQLVSLSGMPAYLSYVSAHAIEAPQREKVALERYALLAR
                     LDRAPSAAAALEELRKLGVAWYVTTGWSGPPWDPDWRQARFFERNVAIYAVPER"
     gene            1171..1863
                     /locus_tag="VFB53_10510"
     CDS             1171..1863
                     /locus_tag="VFB53_10510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN87201.1"
                     /translation="MDPGRRAVPRFWQPDWLVLREMARVLRGLLADPALELRDARVLD
                     FGCGTRPYEAWFAAAGASYRGADIDAAHEVRIRDDGTLDCADGEYDLVASFQVLEHVW
                     DLGAYLGEARRALRAQGWLLLSTHGSWLYHPHPHDYRRWTAEGLQREVESHGFRLVRM
                     EPVVGPLAWTTIFRSLGMAHVLKPLPLVGPPAAAAAAVLLNARAWLEDRITPRAITAV
                     NACTYIALFRRA"
     gene            1866..2252
                     /locus_tag="VFB53_10515"
     CDS             1866..2252
                     /locus_tag="VFB53_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZN87202.1"
                     /translation="MLAGRFFSVGLLCALLHNAIMIGGDRLGLHYVASSLVSFAIVVL
                     VGYLLHSFWTFPGAQRGVISFARYTLTMALNLPLSIAGMFLLVDLAGLGVPIAAPAVT
                     VMLAAFNFVGGRWALRGRRPQSTRQT"
     gene            complement(2231..3856)
                     /locus_tag="VFB53_10520"
     CDS             complement(2231..3856)
                     /locus_tag="VFB53_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87203.1"
                     /translation="MRHASVKTRALVLAGVLAGVLCLAFVLLYTGFPRMPERLYIFDY
                     LLRVQDVPGAAVLILIAVAAGWPPLARAGLALIEAIGRKPWATALVTFLVLCAGQLLA
                     VKEHALAGDEHLILLQAKAFAAGRLTAQFPPELIPWLVPEPYVNRWLYASPQTGAVVS
                     VYWPGFALLLAPFALFGIPWVCNPLLAAAALVLIGRLAARLTGAPPAAGWAMLLTLAS
                     PAFSGMALGYFSMTAHLFFNLLYVWLLLERRLVLAGIAGSFALVLHNPWPHFLFALPW
                     IGWIAWHDGRRALARLIAGYAPLTLVVGGGWWLLMRKVQGPLWFAPQDGDFLFYWQLQ
                     FARVFTFPDGATLAHRAAELVKLWLWSVPGLTALMAAGWWLTRRKVPLRLLGLSFATT
                     LAGYLMVWFDQGYGWGVRYLHPALAALPILGAAAIVIAQSEALRRYAAAAALLSLVLA
                     TPFRWAQIESFMREHLARRPPFEKDLRQIVFVTADYPYYTQDFVQNDPFLRAPVIFVL
                     SRGRAADEKLVRERFPGARPTHEARYGHVWRVD"
     gene            complement(3838..>4615)
                     /locus_tag="VFB53_10525"
     CDS             complement(3838..>4615)
                     /locus_tag="VFB53_10525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN87204.1"
                     /translation="GHVPYRNPILRAVHAAANRLLGAWVLGGADQVVFESESVRAYFA
                     QRVRFRSPPLLIENGVDTEVFRPCSAAERRARRAQLGIPPDRPLLLFIGRFVEKKGLG
                     VLRELTQQIERARWLFAGWGPMDPTAWGRPNVSVVHRPATGELSALYQAADLLVLPSV
                     GEGFPLSVQEAMACGTPALVGEDTAAGCPQAADALLSERVGGADTTARWAARIEALLA
                     APGELDALRPRVAAFARAHWSWERCVERYAALLRACGTRQ"
BASE COUNT          669 a         1669 c         1622 g          655 t
ORIGIN      
        1 cgtgatcggc ttgttcctgc tctgcgctgc gctgctgcag cgctggctgc cgcgaggctc
       61 gctgcgcccg ctggtggcga gcacgctcct cgccgtcggc gcagtctggt ttcgcgtgca
      121 ggtgttcgcg gtgggatttc ccgcctggct cgcggccgcg gcgctcgccg tgcctgcggt
      181 tcgcgcgcgc aagctgcttt ccgccggtgc cgcgctgctc gccttcctgc tcttcgtcat
      241 cgcgttctac gcggcgaccg acgccgtatt cgcgctgccc gtgtttctcg aagtgctgca
      301 cgaccggcag gagccgaccg cctataccgg cctctacctc gaaatgcgcg acacgcctta
      361 cggcatcccg gcggggatcg tgctcgtgta cgccgccgcc ctgggcgcgc tcgtcgtgct
      421 ctatccgctc gccctgtggc tcgcgcatcg cgcccgggcg ctgcgcgccg gcgacagctt
      481 tccgggcctc gtgctcgccg cgtatctcgc ggtgatgctc accgcgccga tcgacaaatt
      541 ccgggactcc accgaattca cggtgcggcc gttcgtgctc gcttacgcgg ccgtcgcggt
      601 atggaccgta tgcctgctgt gccgcgtatt cgcgttgcac tggccgcagc gcgcgccgca
      661 agcatggcgc acgatgttcg gcgcctgcct cgtcggcgtg gcgctcgcgt ggcacggcac
      721 gccggcgatg ggccttgcca agttcgaatg ggccgcgctg ttctatccca agcgaatcga
      781 cgccggcctc acccaggcgg gacgctacct gcgcgagcac ggccgccccg ggcaggtgtt
      841 cgccgtgcgc gggctgcagc tggggtgggc ggcaaccgat gccgccgtcc agctggtgtc
      901 gctgagcggc atgccggcat acctgtccta tgtctcggcg catgcgatcg aggcccccca
      961 gcgggagaaa gtggcactcg agcgctacgc cctgctcgcg cgcctggacc gtgcgccatc
     1021 cgcggccgcg gcgctcgagg agttgcgcaa gctcggcgtc gcctggtatg tgacgaccgg
     1081 ctggagcggg ccgccctggg acccggactg gcggcaggcg cgcttcttcg agcgcaacgt
     1141 cgcgatctac gcggttccgg aacgctgagc atggatcccg gccgccgcgc cgtgccgcgc
     1201 ttctggcagc ccgactggct ggtgctgcgc gagatggcgc gcgtgctgcg cggcctgctc
     1261 gccgacccgg cgctcgagct gcgcgatgcg cgcgtgctcg atttcggttg cggcacccga
     1321 ccctacgagg cctggttcgc ggccgccggc gcgagctacc gcggggccga catcgatgcc
     1381 gcgcacgagg tgcgcatccg cgacgacggc acgctcgact gcgccgacgg cgagtacgac
     1441 ctggtggctt cgttccaggt gctggaacat gtctgggacc tcggcgccta ccttggcgag
     1501 gcgcgccgcg cgctgcgagc gcagggatgg ctgctgctct ccacgcacgg cagctggctg
     1561 taccatccgc atccgcacga ctaccgccgc tggaccgccg agggcctgca gcgcgaggtg
     1621 gaatcgcacg gcttccgcct ggtgcgcatg gaacccgtgg tcggcccgct cgcctggacc
     1681 acgatcttcc gctcgctcgg catggcccat gttctcaagc cgctgccgct ggtcggaccg
     1741 ccggcggcgg ccgcggcggc ggtcctgctc aacgcgcggg cgtggctcga ggaccgcatt
     1801 acgccgcgcg cgattaccgc ggtgaacgcc tgcacctaca tcgcgctctt ccgccgcgct
     1861 tagcgatgct cgccggccgt ttcttctccg tcggcctgct gtgtgcgctg ctgcacaacg
     1921 cgatcatgat cggcggcgac cggctcggcc tgcactacgt cgcctcgtcg ttggtctcgt
     1981 tcgccatcgt ggtgctggtg ggctacctgc tgcacagctt ctggaccttt cccggtgcgc
     2041 agcgcggcgt gatttccttc gcgcgctaca cgctcaccat ggcgctcaac ctgccgctgt
     2101 ccatcgccgg catgttcctg ctggtcgacc tggccggcct gggcgtgccg atcgcggcgc
     2161 cggcggtcac cgtgatgctg gcggcgttca acttcgtcgg cggccggtgg gcattgcgcg
     2221 gccggcgccc tcaatccacg cgccagacgt gaccgtagcg cgcctcgtgc gtggggcgcg
     2281 cgcccgggaa gcgctcgcgc acgagcttct cgtcggccgc gcgtccgcgg ctcagcacga
     2341 agatcaccgg ggcgcgcagg aacggatcgt tctgcacgaa atcctgcgtg tagtacgggt
     2401 agtccgcggt cacgaacacg atctggcgca ggtccttctc gaacggcgga cggcgcgcga
     2461 ggtgctcgcg catgaaggac tcgatctgcg cccagcgaaa cggcgtggcg agcacgaggc
     2521 tgaggagcgc ggcggcagcg gcgtagcggc gtaacgcttc gctctgcgcg atcacgatgg
     2581 ccgccgcgcc gaggatcggc agcgcggcga gcgcggggtg caggtagcgc acgccccaac
     2641 cgtagccctg gtcgaaccag accatgaggt agcccgcgag cgtggtggcg aacgacagcc
     2701 ccagcagccg caacggcacc ttgcgccgcg tgagccacca gcccgccgcc atcagcgcgg
     2761 tcagcccggg aacgctccac agccagagct tcaccagctc ggcggcgcgg tgcgcgagcg
     2821 tcgcgccgtc gggaaacgtg aacacgcggg cgaactgcag ctgccagtag aaaagaaaat
     2881 cgccgtcctg cggcgcgaac cagagcgggc cctggacctt gcgcatcagc agccaccagc
     2941 cgccgccgac gacgagcgtc agcggcgcgt aaccggcgat cagccgcgcg agcgcgcgcc
     3001 ggccgtcatg ccatgcgatc cagccgatcc acggcagggc aaagaggaag tgcggccacg
     3061 ggttgtgcag gacgagcgca aacgagcccg cgattccggc cagcaccagg cggcgctcga
     3121 gcagcagcca gacgtacagc aggttgaaaa acaaatgcgc cgtcatcgag aaatagccga
     3181 gcgccatgcc gctgaacgcg ggcgaggcga gcgtgagcag catcgcccag cctgccgccg
     3241 gcggcgcgcc ggtgaggcgc gcggcgagcc ggccgatcaa caccagcgca gcggcggcga
     3301 gcagcggatt gcagacccag ggaatgccga acagagcgaa cggcgcgagc agcagcgcga
     3361 accccggcca gtacaccgag accaccgcgc cggtctgcgg cgaggcgtag agccaccggt
     3421 tgacgtaagg ctccggcacg agccagggga tgagctccgg cgggaattgg gcggtcaggc
     3481 ggccggcggc gaaggccttg gcctgcagca ggatcaggtg ctcgtcgccg gcgagcgcat
     3541 gctccttcac cgccagcagc tggcccgcgc acagcacgag aaacgtcacg agcgccgtgg
     3601 cccagggctt gcggccgatc gcctcgatga gcgcaaggcc ggcgcgcgcg agcggcggcc
     3661 agccggcggc gacggcgatc aggatcagca cggctgcgcc cggcacatcc tggacgcgca
     3721 gcaggtagtc gaagatgtaa agccgctccg gcatcctcgg aaacccggtg tacagcagga
     3781 cgaatgcgag gcacagaacg ccggcgagca cgccggccag caccagcgcg cgcgtcttca
     3841 ctgacgcgtg ccgcacgcgc gcagcagcgc cgcgtagcgc tcgacgcacc gctcccagga
     3901 ccagtgcgcg cgggcgaagg ccgcgacgcg cggccgcagc gcgtcgagct cgcccggcgc
     3961 agcgagcagc gcctcgatgc gcgcggccca ccgcgccgtc gtatcggcac cgccgacgcg
     4021 ctcgctcaac agcgcgtcag ccgcctgcgg gcagcccgcg gcggtgtcct cgccgaccag
     4081 cgccggcgtg ccacaagcca tggcctcctg caccgagagc ggaaatccct cgcccacgct
     4141 cggcagcacg agcaggtccg ctgcctgata gagcgcgctc agctctccgg ttgcgggacg
     4201 gtggaccacg ctcacgttgg gccttcccca cgcggtgggg tccatcggac cccagcccgc
     4261 gaacagccag cgcgctcgct cgatctgctg cgtgagctcg cgcagcacgc cgaggccttt
     4321 cttctcgacg aagcggccga tgaacagcag cagcggccga tcgggaggga ttcccagctg
     4381 cgcgcggcgc gcgcgccgtt cggcggcgga gcagggccgg aagacctcgg tatccacgcc
     4441 gttctcgatc agcaacggcg gcgagcgaaa ccgcacgcgt tgcgcgaaat aggcgcgcac
     4501 cgactcgctc tcgaacacca cctggtcggc gccgcccaga acccaggcgc cgagcagccg
     4561 gttcgccgcg gcgtggaccg cgcgaaggat cgggttgcga tacggcacat gaccg
//
LOCUS       DASRIE010000243        11557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2864136, whole genome shotgun sequence.
ACCESSION   DASRIE010000243 DASRIE010000000
VERSION     DASRIE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864136"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="trxB"
                     /locus_tag="VFB53_10530"
     CDS             <1..907
                     /gene="trxB"
                     /locus_tag="VFB53_10530"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZN87205.1"
                     /translation="GYTAAVYAARANLSPVLITGLAQGGQLMTTTDVDNWPADALGVQ
                     GPELMERFQKHAQRFETELVFDQVNRVRLGARPFVLEGDAAVYTCDALIIATGASAKY
                     LGLPSEQSFMGKGVSACATCDGFFFKGQDVAVVGGGNTAVEESLYLANICRKVTLVHR
                     RDKFRAEKIMVDKLMKRVAEGRIALELDATLDEVLGDKSGVSRVRLRHLKSGATKELP
                     VQGLFIAIGHTPNTQIFAGQLEMENGYIVTRNGREGGATVTSVPGVFAAGDVQDHVYR
                     QAVTSAATGCMAALDAEKFLDLQAA"
     gene            916..1449
                     /locus_tag="VFB53_10535"
     CDS             916..1449
                     /locus_tag="VFB53_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZN87206.1"
                     /translation="MADADDREALRRALAGVKPLAAPRRHAPRRPAPPPVPAQTRRDE
                     RAVLAESLSGPLTADEALESGVELSYLRDGMSRQALRRLRRGHWVVQDHLDLHGMNRD
                     EAALQVAAFVRAAAARGLRCVRIVHGKGLGSKNREPVLKGKLRAWLMPRDEVLAYCQA
                     PAAEGGSGALLVLLKAR"
     gene            complement(1442..1780)
                     /locus_tag="VFB53_10540"
     CDS             complement(1442..1780)
                     /locus_tag="VFB53_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZN87207.1"
                     /translation="MKKIEAVVKPFKLDEVREALSEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVPEKQVEPAIEAIVKSARTGKIGDGKIFVTSVEHVVRI
                     RTGETNESAI"
     gene            complement(1809..3440)
                     /locus_tag="VFB53_10545"
     CDS             complement(1809..3440)
                     /locus_tag="VFB53_10545"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZN87208.1"
                     /translation="MAERILIAAAQINCTVGDLPGNAARILETTARARAAGADLVLTP
                     ELSLCGYPPEDLLLREGFIADCRRELERLAAAVRGTALVVGFPELAAGGERYNAAALI
                     RDGRVQAVARKHDLPNYEVFDEKRYFASGAEPCVFELKGVRFGLTICEDVWGPKAPAM
                     AKAAGAQAILSINASPYHKRKQATRHQVFREWIRSTGLPMVYVNLVGGQDELVFDGAS
                     CVLDGRGEVVRQAPFFEEALELFEFAGARPLPGRVAPELAPEAEIYRALCVGTRDYVG
                     KNGFPGALLGLSGGVDSALTLAVACDALGAERVRAVMMPSQYTAQMSLDDAAEMARRL
                     GVRYDVIPIGPVFDAFRAALAPQFAGRAADLTEENLQARVRGTLLMGLSNKFGSIVLT
                     TGNKSEMAVGYSTLYGDMAGGYAVIKDLSKTLVWRLARYRNTLGEVIPENIITRAPSA
                     ELRPGQTDQDSLPPYEVLDGVMEAYMEQNRSPREIAALGFAAADVKRVVGLIRRSEYK
                     RRQAPPGVRITFRGFGKDWRYPITAKYLDDGDLSL"
     gene            3490..4035
                     /locus_tag="VFB53_10550"
     CDS             3490..4035
                     /locus_tag="VFB53_10550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN87209.1"
                     /translation="MKFWLSQLLTQFRFKRRALALLREVVREEPRHAEAWSRIGFLLA
                     GEGRAEEALAAFQRALALDDGDAPSHFNLAFMLQRLGRHAEALPGFARAIEIAPRTDR
                     AWYGLGLSLAALGRLEEAAQKLEEAARLQPKNPYAGYQLAGIWHRLGRIDKLRAEYER
                     VRGFDARVAEVIGLEFGLLKK"
     gene            4040..5152
                     /locus_tag="VFB53_10555"
     CDS             4040..5152
                     /locus_tag="VFB53_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN87210.1"
                     /translation="MDRLRVSASLAGVDAAQWNALAGGNPFLRHEFLHALNDSGCASA
                     RSGWQPQFLLLERGARLAGAMPLYVKTHSRGEYVFDWAWADAYARHGLEYYPKLLGAV
                     PFTPVTGPRLLAADDAARGALAAGALQAAREFSSLHVLFAPQAEARLLEACGMLLRRT
                     VQFHWTNQGYADFDDFLARLSHQRRKNIRQERRRVREAGVSCRWLEGAQIGERDWAFF
                     HRCYANTYAEHGQRPYLSLEFFLRAGGAMPESFLLVLAERGGAPIAAALDVRGGDALY
                     GRYWGALEHVPLLHFEACYYQAIEYAIARGLARFEGGAQGEHKLFRGLLPVETLSAHW
                     LAHPRFASAIEDYLAREAQGIAQYVNELNEHTPFKV"
     gene            5173..5919
                     /locus_tag="VFB53_10560"
     CDS             5173..5919
                     /locus_tag="VFB53_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN87211.1"
                     /translation="MRLKDKVAIVTGGGSGFGAGIAKRFAEEGARVVVNDINAEGGAR
                     VAQAVGGAFVQADVTRAADWAKLVAAATAKFGRLDAVVNNAGWTHRRKPYLEVTEAEF
                     DKVYAVNVKSVYLSALHALPVFRRQGGGCFVNIASTAGLRPRPGLTVYNSSKGAVILL
                     SKSMAGEFGPDKVRVNCVNPVFSPETALSADFAGGTLTEEAKKAFLATIPLGRFSTPL
                     DVANACVYLASDEASMISGTCVEVDGARSV"
     gene            complement(5916..7337)
                     /locus_tag="VFB53_10565"
     CDS             complement(5916..7337)
                     /locus_tag="VFB53_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN87212.1"
                     /translation="MASSSFDQLGLLAPLLQAVRELGYEQPTPVQEQAIPLVLAGRDL
                     KAGAQTGTGKTAAFALPILQRLAPQASASASPARHPVRALILTPTRELAIQVEQSFRD
                     YGKHLPIRSAVVYGGADMNAQILQLRKGVEVLVATPGRLLDHVQSKTVMLNQVGILTL
                     DEADRMLDMGFLPDIRRIIALLPAVRQNLLFSATFPDEIRGLVNSLLRDPAEVQVAAR
                     NAVAELVTHVVHPVAREKKRELLSYLIQTRNLQQVLVFCGTRIGANRLAHQLRKDRIQ
                     ADAIHGDKSQAERLIALEGFKAGKTAVLVATDVASRGLDIEGLPQVINFDIPHSPEDY
                     VHRIGRTGRAGAAGEAISLVAPTDLEALAAIERLIKKKLDRVLVPGFQPDGGTAATLM
                     GREREARPAPRREEAPRREHAPRPMAPRPQQRAQGDPIFSKPYEPLPAGNSAAPKAEA
                     LQPTQSKRRQPQVAALLGGLKRA"
     gene            7364..9265
                     /locus_tag="VFB53_10570"
     CDS             7364..9265
                     /locus_tag="VFB53_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN87213.1"
                     /translation="MILLRNLALSRGPQLLFSGVSLTLERRWRVGLVGANGSGKSSLL
                     ALLAGELAPDAGDCEIQPGMRLARVEQAAPALSRGVLDYLLDADAALRAAEREIAAAE
                     GGGDGEAIAHAHERHAHAGGYTARARALALLAGLGFAPGDETRAVREFSGGFRMRLNL
                     ARALIAPSDLLLLDEPTNHLDLDTILWLEGWLRAYEGMLLVVSHDRDFLDRVCDHCLA
                     ILPAAQGGAMRLYDGGYSQFEGARALELEQQSAAAARQDREIRRITGFVERFRAKASK
                     ARQAQSRLKRLERMARVAPVIAADTYRFQFEEPERQPERILALHEVAAGYGAHRVLSG
                     VELALTSADRVAVIGANGNGKTTLMRLLAGELAPLAGERIEARDIVVGYFAQHQVELL
                     RGEETPLGMLQRLFRGEREQALRDFLGRFGFTGERAERPIGPLSGGEKSRLALAALVK
                     RRPNLLLLDEPTNHLDLEMRRSLAMALQTYSGALVIVSHDRSLLAACADRILVVADGR
                     VREFDGDLDDYRQLKLKDGRAAGPAPGRGSRKDERRAGAAVRADLRQRLRPLEKQLAA
                     IEARLAQLARDAAEIRAALADPALYAGPDKAKVAELGKAEKRLAEELARAEEDWLHAA
                     ARIEAARAG"
     gene            complement(9262..9789)
                     /locus_tag="VFB53_10575"
     CDS             complement(9262..9789)
                     /locus_tag="VFB53_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87214.1"
                     /translation="MKLHQLKVDYHAEEDRLLMLIAASDGVELRMWLTRRFVKLLWPL
                     LVKLAEEASPRIRTQANPEARKALLGIEHQQAVQKADFSKAYEPKSRSTPLGEEPLLL
                     GRIQTGHDRKGQPVVALHPAEGQGVTLTFDSVLLHSVCRLLQAAVKKSGWDMELKLPG
                     AEAAPAEERSSRTIN"
     gene            complement(9808..10359)
                     /locus_tag="VFB53_10580"
     CDS             complement(9808..10359)
                     /locus_tag="VFB53_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZN87215.1"
                     /translation="MPAAQVGPGRPAFERKILAPGDAARWAAALPRPLVFTNGVFDLL
                     HRGHVTYLARARALGASLLVALNGDASVRRLGKGPRRPVNALADRMALIAALEMVDAV
                     TWFDADTPAALIAACRPEVLVKGGDWPVARIVGAREVLARGGAVHSIPFEHQRSTTAL
                     LRRIGGGARARAAAGKKARRKPR"
     gene            complement(10341..10943)
                     /locus_tag="VFB53_10585"
     CDS             complement(10341..10943)
                     /locus_tag="VFB53_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZN87216.1"
                     /translation="MTRLRVMLCGAALALPLLQGCAELIVVGAGMAVATVEDRRSTAS
                     QLEDRGIELRAANRIDDKLGERVHVNATAYNRYLLLTGEVPDEALKAEVEALVRGVPN
                     VLGVTNDLQVAGHSSTTSRSNDTLITSKVKTRLIDANKTNPFQIKVVTEAAVVYLLGV
                     VTEQEAADAVEIARTTSGVRKVVKVFEYCKPGDEVCRPRK"
     gene            complement(10940..11530)
                     /locus_tag="VFB53_10590"
     CDS             complement(10940..11530)
                     /locus_tag="VFB53_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZN87217.1"
                     /translation="MLEQRIQQQFFDSADLKYAAAEVLSKPIADAVSALVGCITGGGK
                     VLACGNGGSAADAQHFAAELVNRFERERAPLAGIALTTDSSALTSIANDYGYEQVFEK
                     QLRALARKGDVLLAISTSGNSASVLAAMRAARELGVRTVALTGRGGGKMGAALGAEDV
                     HVCVPHERTMRIQEVHLLVLHCLCDGIDFQLFGANP"
BASE COUNT         1601 a         4155 c         4132 g         1669 t
ORIGIN      
        1 cggttacacg gccgcagtat acgccgcgcg cgcgaacctt tcgccggtcc tgatcaccgg
       61 actcgcgcag ggcggacagc tgatgaccac caccgacgtc gacaattggc cggcggacgc
      121 gctcggcgtg caggggccgg agctgatgga gcgtttccag aagcacgccc agcgcttcga
      181 gaccgaattg gtgttcgatc aagtgaaccg ggtgcggctc ggcgcgcggc ccttcgtgct
      241 cgagggtgac gccgccgtct acacctgcga cgcgttgatc atcgccaccg gggcctcggc
      301 caagtacctc ggcctgccct cggagcagag cttcatgggc aagggcgtgt cggcctgcgc
      361 cacctgcgac ggatttttct tcaagggcca ggacgtggca gtggtcggcg gcggcaacac
      421 cgcggtcgag gagtcgctgt acctcgcgaa tatctgccgc aaagtcaccc tggtgcaccg
      481 gcgcgacaag ttccgcgccg agaagatcat ggtcgacaag ctgatgaagc gcgtcgccga
      541 aggcaggatc gcgctggagc tcgacgcgac gctcgacgag gtgctcggcg acaagagcgg
      601 cgtctccagg gtgcgcctca ggcacctgaa gagcggcgcg acgaaggagc tgccggtgca
      661 gggcctgttc atcgccatcg gccacacgcc gaacacgcag atcttcgccg gccagctgga
      721 gatggagaat ggctacatcg tgacgcgcaa cgggcgcgaa ggcggcgcca ccgtcaccag
      781 cgtgccgggc gtgttcgccg ccggcgacgt gcaggaccac gtgtatcgcc aggcggtgac
      841 cagcgccgcc accgggtgca tggcggcgct cgacgccgag aagttcctcg acctgcaggc
      901 cgcctgaggc gcgccgtggc ggacgccgac gaccgcgagg cgctgcgccg ggcgctcgcc
      961 ggggtcaagc ccctcgccgc gccgcgccgg cacgcgccgc gccgtcccgc cccgccgccg
     1021 gtgcccgcgc agacgcggcg cgacgagcgc gcggtgctgg ccgaaagcct gtccgggccg
     1081 ctcaccgcgg acgaggcgct ggaatcgggc gtcgagttga gctacctgcg cgacggcatg
     1141 tcgcgccagg cgctgcgccg gctgcggcgc ggccactggg tggtgcagga ccacctcgac
     1201 ctgcacggca tgaaccgcga cgaggcggcg ctgcaggtgg ccgcctttgt gcgcgcggcg
     1261 gccgcgcgcg gcctgcgctg cgtgcgcatc gtccacggca aggggctggg ctcgaagaat
     1321 cgcgagccgg tgctgaaggg gaagctgcgc gcctggttga tgccgcgcga cgaggtactc
     1381 gcctactgcc aggcgccggc cgccgagggc ggcagcggcg cgctgctggt gctgctgaag
     1441 gctagatagc cgattcgttg gtctcgccgg tgcggatgcg caccacgtgc tcgaccgagg
     1501 tgacgaagat cttgccgtcg ccgatcttgc cggtgcgcgc cgacttgacg atcgcctcga
     1561 tcgccggctc gacctgcttc tcgggcacca ccacctcgat cttcaccttg ggcaggaagt
     1621 cgaccacgta ctcggcgccc cggtacagtt cggtgtggcc cttctggcgg ccgaatccct
     1681 tgacctcggt cacggtgagg ccggtgacgc cgacttccga cagcgcctcg cgcacctcgt
     1741 ccagcttgaa aggcttcacg acggcttcaa tcttcttcat gcctggtcct cccgggaatc
     1801 ggccctcatt atagcgacag gtcgccgtcg tcgaggtact tcgcggtgat cgggtagcgc
     1861 cagtccttgc cgaagccgcg gaaggtgatg cgcaccccag gcggcgcctg gcggcgcttg
     1921 tactcgctcc tgcggatcaa ccccaccacg cgcttcacgt cggcggcggc gaaccccagg
     1981 gcggcgatct cgcgcgggct gcggttctgc tccatgtagg cttccatcac gccgtccagc
     2041 acctcgtagg gcggcaggct gtcctggtcg gtctgcccgg ggcgcaattc cgccgagggc
     2101 gcgcgcgtga tgatgttttc cggtatcacc tccccgagcg tgttgcggta gcgcgcgagg
     2161 cgccagacca gggtcttgga gaggtccttg atcaccgcgt agccgccggc catgtcgccg
     2221 tacagggtcg agtagcccac cgccatctcc gacttgttgc cggtggtgag cacgatcgag
     2281 ccgaacttgt tggacagccc catcagcagg gtaccgcgca cccgcgcctg caggttctcc
     2341 tcggtcaggt cggcggcgcg gccggcgaac tgcggcgcga gcgcggcgcg gaaggcgtcg
     2401 aacaccgggc cgatcgggat cacgtcgtag cgcacgccga ggcggcgcgc catctcggcc
     2461 gcgtcgtcca ggctcatctg cgcggtgtac tgcgagggca tcatcaccgc gcgcacgcgc
     2521 tcggcgccga gcgcgtcgca ggccacggcg agggtgagcg ccgagtcgac gccgcccgac
     2581 agtccgagca gcgcgcccgg aaagccgttc ttgccgacgt agtcgcgcgt gccgacgcac
     2641 agcgcacggt agatctccgc ctccggcgcc agctcggggg cgacgcggcc cggcagcggc
     2701 cgcgcgcccg cgaactcgaa cagctccagc gcctcctcga agaacggcgc ctggcgcacc
     2761 acctcgccgc gaccgtcgag cacgcaggac gcgccgtcga acaccagctc gtcctggccg
     2821 cccaccaggt tcacgtagac catcggcagc ccggtgctgc ggatccactc gcggaacacc
     2881 tggtgccgcg tggcctgctt gcgcttgtgg tagggcgagg cattgatcga caggatcgcc
     2941 tgcgcgcccg ccgccttggc catcgccggc gccttgggcc cccacacgtc ctcgcagatg
     3001 gtcaggccga agcgcacgcc cttcagctcg aacacgcagg gctcggcgcc ggaggcgaaa
     3061 tagcgcttct cgtcgaacac ctcgtagttc ggcagatcgt gcttgcgcgc caccgcctgc
     3121 acgcggccgt cgcggatcag cgccgccgcg ttgtagcgct cgccgccggc cgccagctcc
     3181 ggaaaaccga ccaccagcgc ggtgccgcgc accgcggcgg cgagtcgctc gagctcgcgc
     3241 cggcagtcgg cgatgaagcc ctcgcgcagc agcaggtcct ccggcgggta gccgcacagc
     3301 gacagctcgg gcgtcagcac caggtcggcg ccggccgcgc gcgcgcgcgc ggtggtctcg
     3361 aggatgcgcg cggcgttgcc ggggagatcg cccacggtgc agttgatctg cgccgcggcg
     3421 atcaggatgc gttcggccat cacggggatt ctataatggc cgcatggcca ccacggcttc
     3481 gccgcgcccg tgaagttctg gctcagccag ctgctgaccc agttcaggtt caagcgccgc
     3541 gcgctcgcgc tgctgcgcga agtggtgcgc gaggaaccgc gccacgccga ggcctggagc
     3601 cgcatcggct tcctgctggc cggcgagggg cgggcggagg aggcgctcgc cgccttccag
     3661 cgcgcgctgg cgctcgacga cggcgacgcg ccttcgcact tcaacctcgc cttcatgctg
     3721 cagcgcctgg gccggcacgc cgaggccttg cccggcttcg cgcgcgccat cgagatcgcg
     3781 ccgcgcaccg accgcgcctg gtacgggctc gggctgtcgc tcgccgccct cggccggctc
     3841 gaggaagcgg cgcagaagct ggaggaagcg gcgcgcctgc agccgaagaa tccctacgcc
     3901 ggctaccagc tggcgggcat ctggcaccgc ctcggccgca tcgacaagct gcgcgccgag
     3961 tacgagcgcg tcaggggctt cgacgccagg gtggccgagg tcatcgggct cgaattcggc
     4021 ctgctgaaga agtagcgcgt tggaccgcct gcgcgtctcc gcatcgctcg ccggcgtcga
     4081 cgcggcgcag tggaacgccc tcgccggcgg caaccctttc ctgcggcacg agttcctgca
     4141 cgccctgaac gacagcggct gcgccagcgc gcgcagcggc tggcagccgc agttcctgct
     4201 gctggagcgc ggcgcccggc tggccggcgc catgccgctc tacgtcaaga cccactcgcg
     4261 cggcgagtac gtgttcgact gggcctgggc cgacgcctac gcgcgccacg gcctcgagta
     4321 ctacccgaag ctgctcggcg cggtgccgtt cacgccggtg accggcccgc gcctgctcgc
     4381 cgccgacgac gcggcgcgcg gcgcgctcgc cgccggcgcc ctgcaggcgg cgcgcgagtt
     4441 ctcctcgctg cacgtgctgt tcgccccgca agccgaggcg cggctgctcg aggcctgcgg
     4501 catgctcctg cggcgcaccg tgcagttcca ctggaccaac cagggctacg ccgacttcga
     4561 cgacttcctc gcgcgcctgt cgcaccagcg gcgcaagaac atccgccagg agcggcgccg
     4621 cgtgcgcgag gcgggggtca gctgccgctg gctcgaaggc gcgcagatcg gggagcgcga
     4681 ctgggcgttc ttccaccgct gctatgccaa tacctacgcc gagcacggtc agcgccccta
     4741 cctcagcctg gagttcttcc tgcgcgcggg cggcgccatg ccggagagtt tcctcctggt
     4801 gctggcagag cgcggcggcg cgccgatcgc cgcggcgctc gacgtgcgcg gcggcgacgc
     4861 gctctacggg cgctactggg gcgcgctgga gcatgtgccg ctgctgcact tcgaggcctg
     4921 ctactaccag gcgatcgagt acgccatcgc ccgcggactc gcgcgcttcg agggcggcgc
     4981 gcagggcgag cacaagctgt tccgcggcct gttgccggtg gagacgctgt cggcgcactg
     5041 gctggcgcac ccgcgcttcg ccagcgccat cgaggactac ctggcgcgcg aggcgcaggg
     5101 catcgcgcag tacgtgaacg agctgaacga gcacacgccg ttcaaagtat gatttccggt
     5161 cgaggaggaa ccatgaggct caaagacaag gtggccatcg tcaccggcgg cggctcgggc
     5221 ttcggcgccg ggatcgccaa gcgcttcgcc gaggaaggcg ccagggtggt ggtcaacgac
     5281 atcaatgccg agggcggcgc gcgcgtggcg caggccgtcg gcggggcgtt cgtgcaggcc
     5341 gacgtgacca gggcggcgga ctgggcaaag ctggtggcgg ccgccacggc gaagttcggc
     5401 cggctcgacg cggtggtgaa caacgcgggc tggacgcacc ggcgcaagcc ctacctggag
     5461 gtcaccgaag ccgagttcga taaggtctac gctgtcaacg tgaaaagcgt ctacctctcg
     5521 gcgctgcacg cgctgcccgt gttccgccgg cagggcggcg gctgcttcgt caacatcgcc
     5581 tccaccgcag gcctgcgccc gcgccccggc ctgacggtgt acaacagctc caagggcgcg
     5641 gtgatcctgc tgtcgaagtc gatggccggc gagttcgggc cggacaaggt ccgcgtcaac
     5701 tgcgtcaacc cggtgttcag cccggagacc gcgctgtcgg ccgatttcgc cggcggcacg
     5761 ctcaccgagg aggcgaagaa ggctttcctc gccacgatcc cgctggggcg cttctccacg
     5821 ccgctcgacg tggccaacgc ctgcgtctac ctcgcctcgg acgaggcgtc gatgatctcg
     5881 gggacctgcg tcgaggtgga cggcgcgcgc agcgtctagg cgcgcttcaa gccgcccagc
     5941 agcgcggcca cctgcggctg gcggcgcttg gactgggttg gctgcagcgc ctctgccttg
     6001 ggcgctgcgg agttgccggc cggcaacggc tcgtagggct tggagaatat cgggtcgccc
     6061 tgcgcccgct gctgcggccg cggcgccatc gggcggggcg catgctcgcg gcgcggggcc
     6121 tcctcgcggc gcggcgcggg acgcgcctcg cgctcccgcc ccatcagggt cgcggccgtg
     6181 ccgccgtcgg gctggaagcc cggcaccagc acgcggtcga gctttttctt gatcaggcgc
     6241 tcgatcgccg ccagcgcctc gaggtcggtc ggcgcgacca gcgagatcgc ctcgcccgcg
     6301 gcgccggcgc ggccggtgcg gccgatgcgg tgcacgtagt cctcgggcga atgcgggatg
     6361 tcgaaattga tcacctgcgg cagcccttcg atgtccaggc cgcgcgaggc gacgtcggtc
     6421 gccaccagca ccgcggtctt gccggccttg aagccttcca gcgcgatcag ccgttcggcc
     6481 tgcgatttgt cgccatggat ggcgtcggcc tggatgcggt ccttgcgcag ctggtgcgcc
     6541 aggcggttgg cgccgatgcg cgtgccgcag aacaccagca cctgctgcag gttgcgggtc
     6601 tggatcaggt aggacaggag ttcgcgcttc ttctcgcgcg ccaccgggtg caccacgtgc
     6661 gtcaccagtt cggccaccgc gttgcgcgcc gccacctgca cctcggccgg atcgcgcagc
     6721 agggagttca ccaggccgcg gatctcgtcc gggaaggtgg ccgagaacag caggttctgc
     6781 cgcaccgccg gcagcagggc gatgatgcgg cggatgtcgg ggaggaaccc catgtcgagc
     6841 atgcggtcgg cctcgtcgag cgtcaggatg ccgacctggt tgagcatcac ggtcttgctc
     6901 tgcacgtggt ccagcagccg tccgggcgtc gccaccagca cctcgacgcc cttgcgcagc
     6961 tgcaggatct gggcattcat gtcggcgccg ccgtacacca cggcgctgcg gatcggcagg
     7021 tgcttgccgt agtcgcggaa gctctgctcg acctggatcg ccagctcgcg cgtcggcgtg
     7081 aggatgagcg cgcgcaccgg gtggcgcgcg ggcgaggcgc tcgcgctcgc ctgcggggcg
     7141 aggcgctgca ggatcggcag cgcgaaggcc gcggtcttgc cggtgccggt ctgcgcgcct
     7201 gccttcaggt cgcggcccgc gagcaccagc gggatcgcct gttcctgcac cggggtgggc
     7261 tgctcgtaac cgagttcgcg caccgcctgg agcaggggtg caaggaggcc gagctgatcg
     7321 aaggaagaac ttgccaaggg cgcgaattat actccgttcc cccatgatcc tcctgcgcaa
     7381 tctggcgctg tcgcgcgggc cgcagctgct gttctcgggc gtgtcgctca ccctggagcg
     7441 ccgctggcgc gtcggcctgg tcggcgccaa cggcagcggc aagtcgagcc tgctggcgct
     7501 gctcgccggc gagctcgcgc ccgacgcggg cgattgcgag atccaacccg gcatgcggct
     7561 ggcgcgcgtc gagcaggcgg cgccggcgct ctcgcgcggc gtgctcgact acctgctcga
     7621 cgccgacgcg gcgctgcgcg ccgccgagcg cgagatcgcg gcggccgagg gcggcggcga
     7681 cggcgaggcg atcgcgcacg cgcacgagcg ccacgcgcat gccggcggct acacggcgcg
     7741 cgcgcgcgcg ctcgcgctgc tcgccgggct gggcttcgcc cccggcgacg agacgcgcgc
     7801 ggtgcgcgag ttctccggcg gcttccgcat gcgattgaac ctggcgcgcg ccctgatcgc
     7861 gccctcggac ctgctgctcc tggacgagcc gaccaaccac ctcgacctcg acaccatcct
     7921 gtggctggag ggctggctgc gggcctacga gggcatgctg ctcgtggtgt cgcacgaccg
     7981 cgatttcctc gaccgcgtct gcgaccattg cctcgccatc ctgcccgccg cgcagggcgg
     8041 cgcgatgcgc ctgtacgacg gcggctactc gcaattcgag ggcgcacggg ccctggagct
     8101 ggagcagcag tccgccgccg cggcgcgcca ggacagggaa atccggcgca tcaccggctt
     8161 cgtcgagaga ttccgcgcca aggcgagcaa ggcgcgccag gcgcagagcc gcctgaagcg
     8221 cctggagcgc atggcgcgcg tcgcgccggt gatcgccgcc gacacctacc gcttccagtt
     8281 cgaggagccg gagcgccagc ccgagcgcat cctcgcgctg cacgaggtcg ccgccggcta
     8341 cggcgcgcac cgcgtgctct cgggtgtcga gctcgcgctc accagcgccg accgggtggc
     8401 cgtgatcggc gccaacggca acggcaagac cacgctgatg cgcctcctcg cgggcgagct
     8461 cgcgccgctc gccggcgagc gcatcgaggc gcgcgacatc gtcgtcggct acttcgccca
     8521 gcaccaggtg gagctgctgc gcggggagga aacgcccctc ggcatgctgc agcgcctgtt
     8581 ccgcggcgag cgcgaacagg ccctgcgcga tttcctcggc cgcttcggct ttaccggcga
     8641 gcgcgccgag cgcccgatcg ggcccctgtc gggcggcgag aagtcgcgcc tcgcgctcgc
     8701 cgcgctggtg aagcgccggc ccaacctgct gctgctcgac gagccgacca accatctcga
     8761 cctggagatg cgcaggtcgc tcgccatggc gctgcagacc tacagcggcg ccctggtgat
     8821 cgtgtcgcac gaccgcagcc tcctcgccgc ctgcgccgac cgcatcctgg tggtcgccga
     8881 cggccgcgtg cgcgagttcg acggcgacct cgacgactac cgccagctga aattgaagga
     8941 cggacgcgcc gccggcccgg cgcccgggcg cggcagccgg aaggacgagc ggcgcgccgg
     9001 agccgcggtg cgcgcagatc tcaggcagcg gctgcggccg ctggaaaagc agctggcggc
     9061 gatcgaagca cgcctcgcgc agctggcgcg cgatgccgcg gagatccgcg ccgcgctcgc
     9121 cgacccggcg ctctacgcgg gcccggacaa ggcaaaagtc gccgaactgg gcaaggcgga
     9181 aaagcgcttg gccgaggaac tggcgcgcgc cgaggaggac tggctgcacg ccgcggcgcg
     9241 catcgaggcc gcgcgcgcgg gctagttgat ggtgcggctg ctgcgctctt ccgccggcgc
     9301 ggcctcggcc ccgggcagct tcagctccat gtcccagccg ctcttcttca ccgcggcctg
     9361 cagcagccgg cacaccgaat gcagcagcac cgagtcgaag gtcagcgtca cgccctgccc
     9421 ctcggccggg tgcagcgcca ccaccggctg gcccttgcgg tcgtgcccgg tctggatgcg
     9481 cccgagcagc agcggctcct cgccgagcgg cgtgctgcgc gacttgggct cgtaggcctt
     9541 gctgaaatcg gccttctgca ccgcctgctg gtgctcgatg ccgagcagcg ccttgcgcgc
     9601 ttccggattg gcctgcgtgc ggatgcgcgg gctcgcctcc tcggcgagct tcaccagcag
     9661 cggccacagg agcttcacga agcggcgcgt cagccacatg cgcagctcca cgccgtcgct
     9721 ggcggcgatc agcatcagca gccggtcttc ctcggcgtgg tagtccacct tcagctgatg
     9781 cagcttcatc gcggaatgat agcgccgcta gcgcggcttc cggcgcgcct ttttcccggc
     9841 cgcggccctg gcccgggccc cgccgccgat gcggcgcagc agcgccgtgg tcgagcgctg
     9901 gtgctcgaag ggaatcgagt gcaccgcgcc gccgcgcgcc agcacctcgc gcgcgccgac
     9961 gatgcgcgcc accggccagt cgccgccttt caccaggacc tcgggccggc aggcggcgat
    10021 cagggcggcg ggcgtgtcgg cgtcgaacca ggtcaccgcg tccaccatct ccagcgcggc
    10081 gatgagcgcc atgcggtccg cgagcgcgtt caccgggcgc cgcgggccct tgcccaggcg
    10141 ccgcaccgag gcgtcgccgt tgagcgccac cagcagcgag gcgccgagcg cgcgggcgcg
    10201 cgccaggtag gtgacgtggc cgcggtgcag gaggtcgaac acgccgttgg tgaacaccag
    10261 cggccgcggc agcgccgccg cccagcgcgc ggcgtcgccg ggcgcgagga tcttgcgctc
    10321 gaaagcgggc cggccggggc ctacttgcgc ggccggcaca cttcgtcgcc cggcttgcag
    10381 tactcgaaga ccttgaccac cttgcgcacg ccggacgtgg tgcgcgcgat ctccaccgcg
    10441 tcggcggcct cctgctcggt gaccacgccg agcaggtaca ccaccgccgc ctcggtcacc
    10501 accttgatct ggaacgggtt ggtcttgttg gcgtcgatca ggcgcgtctt cactttcgag
    10561 gtgatcagcg tgtcgttgga gcgcgaagtg gtggaggaat ggccggcgac ctgcaggtcg
    10621 ttggtgacgc cgagcacatt gggcacgccg cgcacgagag cctcgacctc tgccttgagc
    10681 gcctcgtccg gcacctcgcc cgtgagcagc aggtagcggt tgtaggcggt cgcgttgacg
    10741 tgcacgcgct cgcccagctt gtcgtcgatg cggttggcgg cgcgcaactc gatgccgcgg
    10801 tcctcgagct gcgaggcggt gctgcgccgg tcctcgacgg tggccaccgc catgcctgcg
    10861 cccaccacga tcagttccgc gcagccctgc aggaggggca gggccagcgc cgcgccgcac
    10921 agcattactc gcaatcgggt catgggttcg ctccgaacag ctggaaatcg atgccgtcgc
    10981 acaggcagtg cagcaccagc aggtgcactt cctggatgcg catggtgcgc tcgtgcggca
    11041 cgcagacgtg cacgtcctcg gcgcccagcg cggcgcccat cttgccgccg ccgcggccgg
    11101 tgagggcgac ggtgcgcacc cccagctcgc gcgccgcgcg catcgccgcc agcacgctgg
    11161 ccgagttgcc cgaggtcgag atcgccagca gcacgtcgcc cttgcgcgcc agcgcgcgca
    11221 gctgcttctc gaacacctgc tcgtagccgt aatcgttggc gatcgaggtg agcgccgagc
    11281 tgtcggtggt gagcgcgatg ccggcaagcg gcgcgcgctc gcgctcgaaa cggttgacca
    11341 gctccgcggc gaaatgctgc gcgtcggcgg ccgagccgcc gttgccgcag gcgagcacct
    11401 tgccgccgcc ggtgatgcag ccgaccagcg cactcaccgc gtcggcgatc ggcttggaga
    11461 gcacctccgc agcggcgtac ttgagatcgg cgctgtcgaa aaactgctgc tgaattcgtt
    11521 gctcgagcat ggtcaccgat gatatgacac gggtgtc
//
LOCUS       DASRIE010000244         9259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1557739, whole genome shotgun sequence.
ACCESSION   DASRIE010000244 DASRIE010000000
VERSION     DASRIE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557739"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFB53_10595"
     CDS             complement(<1..384)
                     /locus_tag="VFB53_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HZN87218.1"
                     /translation="MTPMLAKPTDNILPMESVSRPAGMASGRHIGAILMDEGKLSASD
                     AEQVLKRQRELGWRFGEAAIELNFITDADLREALAKQYEFPYLAAGPEGVSKELVAAW
                     DPFHPVVEELRAVRTQLLVRWFNPGG"
     gene            complement(388..1824)
                     /locus_tag="VFB53_10600"
     CDS             complement(388..1824)
                     /locus_tag="VFB53_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZN87219.1"
                     /translation="MDLSQFLLALRARRKAFVIAFAVTVLTAIAVALILPKKYVATAT
                     LLVDTRDEQTMSPVRMTARERAGYLQTQVDLLKSNRVATQVARDLKLAQKPGVREAWE
                     KDTGGAGLIEEWIAADLLKNIVVDTSVSSFLLVNYPSDDPRFAAEVANGFTKAYLDMV
                     LYLRTEPTREAAVWFDQQLKALRTQVAQAQTKLNAYQKEKGILVEDARIDVESARLSE
                     LSTQLLAARNATYDAVSRYKQATEILASDASPEAIPDVLANPHIISIKTALGAVEARR
                     AQESTVLGENHPQYLRTAAEVQGLREKLQVEVKKVVAALSNAVEQQKKRERELQAALE
                     AQNQRLLTVKDFRIDISAMTRDIENAQRSYDTVLARYMTNKIDATANKANVMLLAPAI
                     EPLKPLHPKVGLISGLAVILGAVLAAALVYVFEMLDRRVRSRSDLESRLAVPSLGLLS
                     KWQPTGGRLLPAPIPSGARAARALPHPW"
     gene            complement(1838..2590)
                     /locus_tag="VFB53_10605"
     CDS             complement(1838..2590)
                     /locus_tag="VFB53_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZN87220.1"
                     /translation="MRQIAVGILGVFLLALAGLAQAQTDKLGPGDAIRVTVHQQPDLT
                     TDARISERGTIDMPLVGEVKLTGMSPQQAAATIATGLKQGKFLKNPQVNVAVTTLRSR
                     QVSVLGHVARPGRYPLEDTSSQLSDVIAAAGGITTTGAQHVTVLRDGKEQRVNVFKPF
                     ALRAGDTVNVERVPVVYIYGEVTRSGAYPIVPDMTVMHAIAAGGGITPRGSERRLKLR
                     RTGPDGKLVETDASLRDAVKPDDVIYVKEALF"
     gene            complement(2590..3396)
                     /locus_tag="VFB53_10610"
     CDS             complement(2590..3396)
                     /locus_tag="VFB53_10610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN87221.1"
                     /translation="MIGRLALIVLLSLPAAACEEAAERAAPRPAAKVNGTEIALEQAR
                     GAAGAGAAQALDKVIDRELLVQQALAARLDRDPQVLHALDDARRQVLAQAYLESRASA
                     AAGSARDEVRAFYEENPALFAERRVYRLRELIVSAPAEMIDVLRAEAAKARELEELAT
                     WLRGRGAKFSYVGTASGLTQPAEQIPLAFLPQLARMNAGEIAVFATPLGASVVQLVHA
                     EAAPLTEQQAAPLIEQFLAGRRRLETAAAEVRRLREVATIEYLGEFKRGN"
     gene            complement(3393..3719)
                     /locus_tag="VFB53_10615"
     CDS             complement(3393..3719)
                     /locus_tag="VFB53_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87222.1"
                     /translation="MKPLLVAIAFAFVAAPALAEPASAPANPQSALYSFADVYRLTVG
                     GPIAGVPGFDPAPEMPVRVAAAQAQPAEPRFVVRTLPQPDKWLLVFAGLALAGWVAHR
                     RLVHAL"
     gene            complement(3904..4770)
                     /gene="epsA"
                     /locus_tag="VFB53_10620"
     CDS             complement(3904..4770)
                     /gene="epsA"
                     /locus_tag="VFB53_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HZN87223.1"
                     /translation="MTTTAESTNPSSAQPAPHAVPFSPGALPGDGNGGDARSAATDFL
                     QIESIVLNLDASLRVHARAHFFSWTQGLLQSLIRHELLICTLCLGKPPAFRADGFSMT
                     TPDPTVFSDAFLRDTSVAPALLKAWEEKRYQPVTLDVANGNLGSGAFARELERLGGTQ
                     LLVHGVHDADGRALTLFTFACRPGSIGPRQTYLAQLLAPSLHAAWVRTQLQRSTGAVE
                     KASGQSVLTVRELDILKWIYLGKSNFEIGAILKISPLTVKNHVQKILRKLNVVNRTQA
                     IGKALELRILNP"
     gene            4948..6324
                     /locus_tag="VFB53_10625"
     CDS             4948..6324
                     /locus_tag="VFB53_10625"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HZN87224.1"
                     /translation="MRSQPGAGALLRAALDPLVAIATLAAAAAAFGAGFDGAVLILAL
                     LVFAMTFPGGLTGESARASARAGDLMLEIVTGWVAIVGLLGLLGWATQTLEVFDPRVM
                     LAWALATPAALFFAHRMLPRVLPRVLATQGLQKVAVVAGANELGRRLAERLAADPMLG
                     IRVAGYFDDRASGRLQGLPAAQNLGGLSRLADHVRAQRVDVIYIALPMASQPRILRLL
                     EDLRDTTASIYFVPDIFVSDLIQARVDSIGGIPVVAVCETPFYGFNGVVKRTSDIVLS
                     LCILLLISPAMLAIALGVKLSSPGPVLFKQRRYGLDGRKIVVYKFRTMTVAEDGDVVR
                     QATRNDSRVTKFGAFLRRTSLDELPQFINVLQGRMSVVGPRPHAVAHNEMYRKLIRGY
                     MIRHKVRPGVTGLAQVNGWRGETETVEKMKGRIEMDLRYLRNWSLLLDLQIILKTVVV
                     VLGRQNAY"
     gene            6314..6484
                     /locus_tag="VFB53_10630"
     CDS             6314..6484
                     /locus_tag="VFB53_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87225.1"
                     /translation="MRIELLAVWMLLMFASFGVLAWIALDALRRRPQVERRVVVLLVQ
                     QRKRLCAVSSRR"
     gene            6460..8244
                     /gene="glmS"
                     /locus_tag="VFB53_10635"
     CDS             6460..8244
                     /gene="glmS"
                     /locus_tag="VFB53_10635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZN87226.1"
                     /translation="MCGIVAAVARRNIVPVLIEGLRRLEYRGYDSAGVAVINGELRRV
                     RSCGRVADLERLTDAQHLEGTIGIGHTRWATHGAPSERNAHPHVSGGISVVHNGIIEN
                     HDAIRARLKAAGYEFTSDTDTEVIAHLIHSRGGNDLFAAVQAAVSELKGAYAIAVIAA
                     SDPARMVVARMGAPLLLGMGDGENFAASDTAALLQVTRSIVYLEDGDCAEVTPGAVRI
                     ADARGHRVERPLQVSRLSADAIDLGSYRHYMQKEIFEQPAAVYATLQERFGYSAEQAL
                     RCVDGVLIVACGTSYHAGLAARYWLESLAGVPCSVEIASEYRYREPVANPRALVVAIS
                     QSGETADTLAALQYAKSLGHRHTLAICNVAESALVRAAQLTFLTRAGPEIGVASTKAF
                     TAQLAALLMLAAVLARVRGRLAAEKQILQALRQLPAAVANALRLEPEMAAWAAKFAQF
                     EHALFLGRGVHYPIALEGALKLKEISYIHAEAYAAGELKHGPLALVDARMPVVAIAPR
                     DALLDKLKSNLQEVRARGGELYVLSHGDTAVDDADGIHVVRMHDAPGILSPIVSTVPL
                     QLLAYHAALHRGNDVDKPRNLAKSVTVE"
     gene            complement(8245..8955)
                     /gene="phoB"
                     /locus_tag="VFB53_10640"
     CDS             complement(8245..8955)
                     /gene="phoB"
                     /locus_tag="VFB53_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014110935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZN87227.1"
                     /translation="MGGSILVVDDEAAVRDLLAANLQRAGYKVMCAGDVPQAEALVRD
                     TRPDLVLLDWVLPGIPGLTFARRLRSDQRTKDISIVLLTARAEEQDKVTALESGADDY
                     VTKPFSARELLARIKAVMRRRAPQLADDVVEIAGLRIDPAAHQVSAGDKAIDLWTTEL
                     RLLHFFMTHPGRVFSRARLLDEVWGEHVFVEERTVDVHIRRLRQALAPTGHDKLIETV
                     RGTGYRFKPDLSAANIAN"
BASE COUNT         1322 a         3230 c         3247 g         1460 t
ORIGIN      
        1 gccgccgggg ttgaaccagc gcaccaggag ctgcgtgcgc accgcgcgca gctcctcgac
       61 caccgggtgg aacgggtccc acgcggcgac cagctccttg ctcacgcctt ccgggccggc
      121 ggcgaggtag ggaaactcgt actgcttggc gagcgcctcg cgcaggtcgg cgtcggtgat
      181 gaagttgagc tcgatcgccg cctcgccgaa gcgccagccc agctcgcgct ggcgcttcag
      241 gacctgctcg gcgtcggagg cggacagctt gccctcgtcc atcaggatgg caccgatgtg
      301 gcgccccgag gccattccag cggggcggga aacgctttcc atcggcagga tgttgtcagt
      361 cggcttggct agcatggggg tcaccggtta ccagggatgg ggaagggcgc gagcggcgcg
      421 cgcgccggaa ggaatcggcg ccggcagcag ccggccgccg gtcggctgcc acttcgacag
      481 caggccgagc gagggcacgg cgagccgcga ctcgaggtcg gagcgcgagc gcacgcggcg
      541 gtcgagcatc tcgaacacgt acaccagcgc cgcggcgagc accgcgccca ggatcacggc
      601 cagcccggaa atcaggccga ccttggggtg cagcggcttc agcggctcga tcgccggcgc
      661 gagcagcatc acgttcgcct tgttcgcggt ggcgtcgatc ttgttggtca tgtagcgcgc
      721 cagcaccgtg tcataggagc gctgcgcgtt ctcgatgtcg cgcgtcatcg ccgagatgtc
      781 gatgcggaag tccttcacgg tgagcaggcg ctggttctgc gcctcgagcg ccgcttgcag
      841 ctcgcgctcg cgcttcttct gctgctcgac cgcgttgctg agcgccgcca ccaccttctt
      901 cacctcgacc tgcagcttct cgcgcagccc ttgcacttcg gccgcggtgc gcaggtactg
      961 gggatggttc tcgcccagca cggtgctttc ctgcgcgcgg cgcgcttcca ccgcgccgag
     1021 ggcggtcttg atgctgatga tgtgcgggtt cgccagcacg tccggaatcg cctcgggcga
     1081 ggcgtcgctc gccaggatct cggtcgcctg cttgtagcgg ctcaccgcgt cgtaggtcgc
     1141 gttgcgcgcc gccagcagct gggtggagag ctcggagagc cgcgccgact cgacgtcgat
     1201 gcgcgcgtcc tcgacgagga tgcccttctc cttctggtag gcgttcagct tggtctgcgc
     1261 ctgcgccacc tgcgtgcgca gcgccttcag ctgctggtcg aaccacaccg ccgcctcgcg
     1321 cgtcggctcg gtgcgcaggt agagcaccat gtcgaggtag gccttggtga agccgttggc
     1381 cacctcggcg gcgaagcgcg ggtcgtccga gggatagttg accagcagga agctgctcac
     1441 cgaggtgtcg acgacgatgt tcttcagcag gtccgccgcg atccactcct cgatcaggcc
     1501 ggcgccgccg gtgtcctttt cccaggcttc gcgcaccccg ggcttctgcg caagcttcaa
     1561 atcgcgcgcc acctgcgtcg cgacgcggtt gctcttcagg aggtcgacct gcgtctgcag
     1621 gtagccggcg cgctcgcgcg ccgtcatgcg caccgggctc atggtctgct cgtcgcgcgt
     1681 gtcgacgagc agcgtcgcgg tggcgacgta cttcttcggc aggatcagcg ccactgcgat
     1741 cgcggtgagc accgtcaccg cgaaggcgat gacgaaggcc ttgcgccgcg cacgcagagc
     1801 gagaaggaat tggctcaggt ccatttgcat tgactcccta gaacagtgct tccttgacat
     1861 agatgacgtc gtcgggcttg acggcgtcgc gtaggctggc gtcggtctcg acgagcttgc
     1921 cgtcggggcc ggtgcggcgc agcttcagcc gccgctcgct gccgcgcggc gtgatgccgc
     1981 cgcccgcggc gatggcgtgc atcacggtca tgtcgggcac gatcggatag gcgccggagc
     2041 gcgtcacctc gccgtagatg tagaccaccg gcacgcgctc gacgttgacg gtatcgcccg
     2101 ccctcaaagc gaagggcttg aacacgttca cgcgctgctc cttgccgtcg cgcagcaccg
     2161 tcacgtgctg cgcgccggtg gtggtgatgc cgccggcggc ggcgatcacg tcggagagct
     2221 gcgagctggt gtcctcgagc ggatagcgcc cggggcgcgc cacgtggccc agcaccgaca
     2281 cctgacggct gcgcagcgtg gtcaccgcca cgttcacctg cgggttcttc aggaacttgc
     2341 cctgcttcag cccggtggcg atggtcgccg ccgcctgctg cggcgacatg ccggtcagct
     2401 tcacctcgcc caccagcggc atgtcgatgg tgccgcgctc gctgatgcgc gcgtcggtgg
     2461 tgaggtccgg ctgctggtgc accgtgacgc ggatcgcgtc gccggggccc agcttgtcgg
     2521 tctgcgcctg cgcgagcccg gccagcgcca gaaggaaaac gcccaaaatg cccactgcga
     2581 tctgtctcat cagttgcctc tcttgaattc acccaggtat tcgatggtgg ccacctcgcg
     2641 cagccgccgg acctcggcgg cggccgtctc gagccgcctg cgcccggcga ggaactgctc
     2701 gatcagcggc gccgcctgct gctcggtgag cggcgccgcc tccgcgtgca ccagctggac
     2761 cacggaggcg cccagcgggg tcgcgaaaac ggcgatctcg cccgcgttca tgcgcgcgag
     2821 ctgcggcagg aaagcgagcg gaatctgctc cgcgggctgc gtcagcccgc tcgcggttcc
     2881 gacgtagctg aacttcgcgc cgcggccgcg cagccacgtc gccagctcct cgagctcgcg
     2941 ggccttggcg gcctcggcgc gcagcacgtc gatcatctcc gccggcgccg acacgatcag
     3001 ctcgcgcagc cggtagacgc gccgctccgc gaacagcgcc gggttctcct cgtagaacgc
     3061 gcgcacctcg tcgcgcgcgc tgcccgccgc ggcgctggcg cggctctcga ggtacgcctg
     3121 cgccagcacc tggcggcgcg cgtcgtcgag cgcgtgcagc acctgcgggt cgcggtccag
     3181 gcgcgcggcg agcgcctgct gcaccagcag ctcgcggtcg atcaccttgt cgagcgcctg
     3241 cgccgcgccg gcgccggcgg cgccgcgcgc ctgctcgagc gcgatctcgg tgccgttcac
     3301 cttggcggcc ggccgcggcg cagcgcgctc ggcggcctcc tcgcaggcgg cggcaggaag
     3361 ggaaaggagc acgatcaggg cgagccttcc gatcacagag cgtgcacgag gcgtcggtgc
     3421 gccacccagc ccgcgagcgc gaggccggcg aacaccagca gccacttgtc gggttgcggc
     3481 agcgtgcgca ccacgaagcg cggctcggcc ggctgcgcct gcgccgccgc gacgcgcacc
     3541 ggcatttcgg gcgccgggtc gaaccccggc acgccggcga tcgggccgcc tacggtcagg
     3601 cggtacacgt cggcgaacga atacagggcc gactggggat tggcgggcgc ggacgcgggc
     3661 tcggcgaggg ccggagccgc gacgaaagcg aaagcaatgg cgacgagcag cggtttcatg
     3721 tcaggacccg ggaatgctag atgcggggta ttacggtgcg gacgcgcgct ttactgcgcg
     3781 cgatgcgccg tgcggactaa tgtcgccgga aagggtcggg gcgcgagtcg gaaatgaaaa
     3841 ttcgggaacg cgaaaaaaga aaaccggccg cagccggttt tcgcgatgct cctgcccgcc
     3901 cgctcaggga ttgaggatgc gcagctcgag cgccttgccg atcgcctgcg tgcggttcac
     3961 cacgttcagc ttgcgcagga tcttctgcac gtggttcttc accgtgagcg ggctgatctt
     4021 gaggatcgcg ccgatctcga agttcgactt gccgagatag atccacttca ggatgtcgag
     4081 ctcgcgcacc gtgagcacgc tctggccgga ggccttctcc accgcgcccg tgctgcgctg
     4141 cagctgcgtg cgcacccacg ccgcgtgcag cgacggcgcg aggagctgcg cgagataggt
     4201 ctggcgcggc ccgatgctgc ccgggcggca ggcgaaggtg aagagcgtca gcgcgcgccc
     4261 gtcggcgtcg tgcacgccgt gcaccaggag ctgcgtgccg ccgaggcgct cgagctcgcg
     4321 cgcgaacgcg ccgctgccca ggtttccatt cgccacgtcg agcgtgaccg gctggtagcg
     4381 cttctcttcc catgccttga gcagcgcggg cgcgaccgag gtgtcgcgca ggaacgcgtc
     4441 ggagaacacg gtcgggtcgg gcgtggtcat ggagaagccg tcggcgcgaa acgcgggcgg
     4501 cttgcccagg cacagcgtgc agatcagcag ctcgtggcgg atcaggctct gcagcagacc
     4561 ctgggtccag ctgaagaaat gcgcgcgcgc gtgcacgcgc agcgacgcgt cgaggttgag
     4621 cacgatcgat tcgatctgca ggaagtcggt ggccgcggag cgcgcgtcgc cgccgttgcc
     4681 atcgccggga agcgcgcccg gcgagaacgg aacggcatgg ggcgccggct gcgccgacga
     4741 agggttggtg ctctctgctg tagtagtcat tggcatggcg gcgctcaata tagttgattg
     4801 gaattacgca atagttcgtc cggtacaggc ctcaatcgct agtccggtga gcctacgaag
     4861 cgacggtccg ggcaacgtca gcgttgaacc gcgctcgcct aacctgcggt tgccttgcaa
     4921 gcgactcccg aaacccggac ccgactgttg cgcagccagc ccggcgcggg cgcgctgctg
     4981 cgcgcggcgc tcgacccgct ggtggcgatc gcgacgctcg ccgcggccgc ggccgcgttc
     5041 ggcgccggct tcgacggcgc ggtgctgatc ctggcgctgc tcgtgttcgc gatgaccttt
     5101 cccggcggcc tcacggggga aagcgcgcgc gcctcggcgc gcgccggcga cctgatgctc
     5161 gagatcgtca ccggctgggt ggcgatcgtg ggcctgctcg gcctgctcgg ctgggcgacg
     5221 cagacgctcg aggtgttcga cccgcgcgtg atgctcgcct gggccctggc gacgcccgcg
     5281 gcgctgttct tcgcgcaccg catgctgccg cgcgtgctgc cgcgcgtgct cgcgacgcag
     5341 ggcctgcaga aggtcgcggt ggtcgcgggc gccaacgagc tcggccgccg gctcgccgag
     5401 cggctcgccg ccgacccgat gctcggcatc cgcgtcgccg gctacttcga cgaccgcgcg
     5461 agcggccgcc tgcagggcct gcccgcggcg cagaacctcg gcggcctgtc gcggctcgcc
     5521 gatcacgtgc gcgcgcagcg cgtcgacgtg atctacatcg ccctgccgat ggcctcgcag
     5581 ccgcgcatcc tgcgcctgct cgaggacctg cgcgacacca ccgcgtcgat ctacttcgtg
     5641 cccgacatct tcgtctcgga cctgatccag gcgcgcgtcg actcgatcgg cggcatcccg
     5701 gtggtcgcgg tgtgcgagac gcccttctac ggcttcaacg gcgtggtcaa gcgcaccagc
     5761 gacatcgtgc tttcgctttg catcctgctg ctgatttccc cggcgatgct cgcgatcgcg
     5821 ctcggcgtga agctctcctc gcccgggccg gtcctgttca agcagcgccg ctacgggctc
     5881 gacgggcgca agatcgtggt ctacaagttc cgcaccatga cggtcgccga ggacggcgac
     5941 gtggtgcgcc aggcgacgcg caacgacagc cgcgtgacca agttcggcgc gttcctgcgc
     6001 cgcacctcgc tcgacgagct gccgcagttc atcaacgtgc tgcagggccg catgagcgtg
     6061 gtcgggccgc gcccgcacgc ggtcgcgcac aacgagatgt accgcaagct catccgcggc
     6121 tacatgatcc gccacaaggt gcgcccgggc gtgaccgggc tcgcgcaggt gaacggctgg
     6181 cgcggcgaga ccgagacggt cgagaagatg aagggccgca tcgagatgga cctgcgctac
     6241 ctgcgcaact ggtcgctgct gctcgacctg cagatcatcc tgaagaccgt ggtggtggtg
     6301 ctggggcggc agaatgcgta ttgagctcct cgcggtgtgg atgctcctca tgttcgcgtc
     6361 gttcggcgtg ctcgcctgga tcgcgctcga tgcgctgcgc cggcgcccgc aggtcgagcg
     6421 ccgcgtggtc gtcctgctgg tccagcaaag gaaacgccta tgtgcggtat cgtcgcggcg
     6481 gtagcccgcc gcaacatcgt cccggtcctg atcgaggggc tgcggcgcct ggagtaccgc
     6541 ggctacgact ccgccggcgt ggccgtgatc aacggcgagc tcaggcgcgt gcgctcgtgc
     6601 gggcgcgtgg cggatctcga gcgcctcacc gacgcgcagc atctcgaggg cacgatcggc
     6661 atcggccata cgcgctgggc gacgcacggc gcgccttcgg agcgcaacgc gcacccgcac
     6721 gtgagcggcg gcatctcggt ggtgcacaac ggcatcatcg agaaccacga cgcgatccgc
     6781 gcgcgcctca aggccgccgg ctacgagttc acctcggaca ccgacaccga ggtgatcgcg
     6841 cacctgatcc attcgcgcgg cggcaacgac ctgttcgcgg cggtgcaggc cgcggtgtcc
     6901 gagctgaagg gcgcctacgc catcgcggtg atcgccgctt ccgatccggc ccgcatggtc
     6961 gtcgcccgca tgggcgcgcc gctgctcctc ggcatgggcg acggcgagaa cttcgccgcc
     7021 tcggacaccg ccgcgctgct gcaggtcacg cgcagcatcg tgtacctcga ggacggcgac
     7081 tgcgccgagg tcacgccagg cgcggtgcgc atcgccgacg cgcgcggcca tcgggtggag
     7141 cggccgctgc aggtgagccg cctgagcgcg gatgccatcg acctcggctc ctaccgccac
     7201 tatatgcaga aggagatctt cgagcagccc gccgcggtgt acgccacgct gcaggagcgc
     7261 ttcggctaca gcgccgagca ggcgctgcgc tgcgtcgacg gcgtgctgat cgtcgcgtgc
     7321 ggcacgagct accacgcggg gctcgccgcg cgctactggc tcgagtcgct cgccggcgtg
     7381 ccctgctcgg tcgagatcgc gagcgagtac cgctaccgcg agccggtggc gaacccgcgc
     7441 gcgctggtcg tcgcgatctc gcagtcgggc gaaaccgccg atacgttggc cgcgctgcag
     7501 tacgccaaga gcctcggcca ccgccatacc ctcgccatct gcaacgtggc ggaatcggcg
     7561 ctggtgcgcg ccgcgcagct caccttcctg acgcgggccg gtcccgagat cggcgtcgcc
     7621 tcgaccaagg cctttaccgc gcagctcgcc gcgctgctga tgctcgccgc cgtgctcgcg
     7681 cgggtgcggg gccggctcgc cgccgagaag cagatcctgc aggccctgcg ccagctgccc
     7741 gcggcggtgg cgaacgcgct gcgcctcgag cccgagatgg cggcgtgggc ggccaagttc
     7801 gcgcagttcg agcatgcgct gttcctcggg cgcggggtgc actacccgat cgcgctcgaa
     7861 ggagcgctca agctgaagga gatctcgtac atccacgccg aggcctacgc cgccggagag
     7921 ctcaagcacg gcccgctcgc gctggtcgat gcgcgcatgc cggtggtggc gatcgcgccg
     7981 cgcgacgcgc tgctcgacaa gctcaaatcg aacctccagg aagtgcgcgc caggggcggc
     8041 gagctgtatg tgctgtctca tggggacact gcggtggacg acgcggacgg catccatgtg
     8101 gtgaggatgc acgatgcccc cgggattctt tcgcccatcg tgagcaccgt gccgctccag
     8161 ttgctcgcct atcacgccgc gctccaccgc gggaacgacg tcgataagcc gcggaacctg
     8221 gcgaaatccg ttaccgtcga gtaatcagtt cgcgatgttg gccgcgctca aatccggctt
     8281 gaagcggtag ccggtcccgc gcaccgtctc gatcagcttg tcgtggccgg tgggcgcgag
     8341 cgcctggcgc aggcgccgga tgtgcacgtc caccgtgcgc tcctcgacga acacgtgctc
     8401 gccccagacc tcgtccagca gccgcgctct ggagaagacg cgccccgggt gcgtcatgaa
     8461 gaagtgcagc aggcgcagct ccgtggtcca caggtcgatc gccttgtcgc cggcgctcac
     8521 ctggtgcgcc gccgggtcga tgcgcaggcc cgcgatctcc accacgtcgt cggcgagctg
     8581 cggcgccctg cggcgcatga ccgccttgat cctcgccagc agctcccgcg ccgagaacgg
     8641 cttggtcacg tagtcgtcgg cgccgctttc cagcgccgtg accttgtcct gctcctcggc
     8701 gcgcgccgtc agcagcacga tcgagatgtc cttggtgcgc tggtcggagc gcagccgccg
     8761 cgcgaaggtc aggccgggga tgccgggcag cacccagtcg agcagcacca ggtcgggccg
     8821 cgtatcgcgg accagggcct cggcctgcgg cacgtctccc gcgcacatca ccttgtatcc
     8881 cgcgcgttgc aggtttgcag ccagcagatc gcggaccgca gcttcgtcgt caaccaccag
     8941 aatggaaccg cccattggcg tctcccgttg ttaagggcga ggccatgcta tgtcgcggcc
     9001 ggagggaggc atatcgctgc ccccttattt aacagtcgtg tcatatcggc cggctgtcgt
     9061 tgctcaccga caggccgaaa agaccgccgc gtgttaggca actcttcgta aagacgcggg
     9121 tttcggcgat tagaagccct tggcgcgcag ccagcgccgc gccaggtaga cgaacccggc
     9181 gaacagcact accgccgcga cgagcgccca ccagctcacg ctcgaggcgt cgtccatggc
     9241 gcgggtgagc tggtcgccg
//
LOCUS       DASRIE010000245         8906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_1810233, whole genome shotgun sequence.
ACCESSION   DASRIE010000245 DASRIE010000000
VERSION     DASRIE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810233"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFB53_10645"
     CDS             complement(<1..697)
                     /locus_tag="VFB53_10645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN87228.1"
                     /translation="MLPEPEAEARAASALLIERIRAELRTSGGWIPFSRYMELALHEP
                     GLGYYAGGAAKLGAPGDFATAPEMGTLFARTLARQVAALLQPGEAILEFGAGSGALAE
                     ALIGELSPENYLILETSAELRARQEARLGKRARWIDRLPERFRGVMLANEVADAMPVH
                     ALAWRGNTIMERGVDDKFAWAERPASGELLAAARRLEVPVPYESEIGLVARAWMKLLA
                     ARLERGALLVVDYG"
     gene            726..1475
                     /locus_tag="VFB53_10650"
     CDS             726..1475
                     /locus_tag="VFB53_10650"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HZN87229.1"
                     /translation="MTEASLESKVALVTGAARRVGAAIARRLHAAGASVVIHYRGADA
                     DAAALEKELNAARAGSAMKVKGDLLAPVAPKALVDAALQRFARLDFLVNNASTFYPTA
                     LGQIEPGHWEELMGSNLRAPLFLAQAAAPHLALAGGAIVNIADIHAERPLKGYVVYSL
                     AKAGLVALTRSLALELAPAVRVNAVAPGAIAWPEDGQFASGERERIIATTPLARTGAP
                     GEIAQAVHFLCTAPFVTGQVLAVDGGRSIFL"
     gene            1493..2302
                     /gene="ttcA"
                     /locus_tag="VFB53_10655"
     CDS             1493..2302
                     /gene="ttcA"
                     /locus_tag="VFB53_10655"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZN87230.1"
                     /translation="MHVVKLHRDPRKEAYEAGKLAKRLRRKVGEAIADFGMIEAGDKV
                     MVCLSGGKDSYAMLDVLLALRDKAPVPFELVAVNLDQKQPGFPAQVLPEYLAGLGVPY
                     RIVEQDTYSVVKRVLPEGKTMCSLCSRLRRGVLYRVAGELGATKIALGHHRDDILATF
                     FLNLFFAGRLKAMPPKLKSDDGRHIVIRPLAYVKEDELAAYAAEKGFPIIPCNLCGSQ
                     EHLQRRQVRELLRQWEKQHAGRVESILRALTDVRASHLLDRKLFDFGGLDA"
     gene            2299..3252
                     /locus_tag="VFB53_10660"
     CDS             2299..3252
                     /locus_tag="VFB53_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN87231.1"
                     /translation="MKWIAGLLCAACALPAQAQPQEWPQRPVRMLVGFGAGGGTDILA
                     RIVAPAMGAALGQPVVVENRPGAGGSIAANAVAKAEPDGYTAFLLNNGHAVSAVMLPS
                     LPYDPVADFQPVSMVATMPLVILSGPRAGFGSLQGLLEVARHNPGKLNLASVGIGSTQ
                     HFAAELLFHTAAVKLVHVPYKGTPNAVAATVSNETQVLMETVGSVIGQIRGQAVQALA
                     VTSSTRFPGLPQVPTVAEAGLADYAVTTWYALVFPAKTPAAVVEKTRAALRAALAGDE
                     VQKQMLNVAFLPSESTPAELGAHLKAEIARWGAVRAKAGIK"
     gene            3261..4358
                     /gene="gtdA"
                     /locus_tag="VFB53_10665"
     CDS             3261..4358
                     /gene="gtdA"
                     /locus_tag="VFB53_10665"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HZN87232.1"
                     /translation="MKPVEGASRQAQELEGIVPTVNLAAEEGRKDFYERAKRQNLAPL
                     WRVLHGLVTEKPATRAVPALWRYAEVRPHLMEACKLIGAAEAERRVMVLENPGLAGQS
                     RITQSLFAGLQIILPGEIAPAHRHAASALRFIVEGKDAFTAVAGERTMMAPGDFVITP
                     SWTWHDHGNEGAGPMVWLDGLDMHIVNLLSASFREDLGGRAQALERPEGAAPAEFGNN
                     LLPVDSVHRSQTSPIFNYPYRKSRESLDALARTRAPDPWHAHKMKYINPVNGGWAMPT
                     ISTWLQLIPKGFRSAPYRATDSTVFTVVEGRGRSTIGDESFDWGPHDIFVAPSWMAHA
                     HEAAEDAVLFLYSDRVVQEKLDLFREAKERG"
     gene            4370..5116
                     /locus_tag="VFB53_10670"
     CDS             4370..5116
                     /locus_tag="VFB53_10670"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HZN87233.1"
                     /translation="MLGAALATVGLAIAVAACARLGNDWRTASREPVGLAPAPAATPE
                     AVVQVYAARTVGMRGLFGVHSWVAVKPARARAYTVYEVIGWKLRWDDTAVSIREREPD
                     ARWFGNEPELLAEARGADAEPLIPRIEAAVKSYPYPDEYRAWPGPNSNTFTAWVMRRV
                     PELRADLPPTAVGKDYLSGSLFASAPSGSGMQLSLVGLLGITASSVEGLEINILGMTF
                     GVDPWPPAIKLPIAGRLGMASGRSAGNATP"
     gene            5163..6662
                     /locus_tag="VFB53_10675"
     CDS             5163..6662
                     /locus_tag="VFB53_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN87234.1"
                     /translation="MNCAEQLVEIFGSQADVARAFQLDRAVVHNWVKTGYVPARWAGE
                     VERVTQGRINASDVVNEASAKNPVRVKSRPDDAPFGIPPENQAMSQYAPSKRITSFHP
                     PQRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFVEMMEELKSLLRYVYQTKNPLTFPL
                     SGPGSVGMEFCFVNLVAPGDKVIVCQNGVFGGRMLENVIRCGGTPIVVEDKWGEPVDP
                     QKVEDALKKNKGVGVLAFVHAETSTGCQSDARELARLAHKHGALVIVDAVTSLAGTPV
                     KVDEWELDAIYSASQKCLSCTPGLSPVSFSERVVERVKKRKDKIHSWFMDMNLLLGYW
                     NAGARTYHHTAPTNSLFALHEALLLIREEGLENAWARATRHHLALKAGLEAMGLKFLV
                     QEEYQLPQMNAVLCAEGVDEAQVRSRLLSEFGLEIGAGLGPLKGKIWRFGLMGYSCRP
                     DNVMLCLSALGSVLDDMGYAVHVGDAESAAHGAYAQMHVKDAQRKKRAA"
     gene            6675..7043
                     /locus_tag="VFB53_10680"
     CDS             6675..7043
                     /locus_tag="VFB53_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87235.1"
                     /translation="MEHSIEADEEFLRVRVSGRDTNTPPSEVCAEVLRESVRLGRMRI
                     LIELDQKFPLSPASQYQLVTRLPELGLMAQHRIALVHRKPEAQKANEFIDVVAGNRGI
                     QMRNFPSVEEAKAWLRTQAA"
     gene            7057..8100
                     /locus_tag="VFB53_10685"
     CDS             7057..8100
                     /locus_tag="VFB53_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN87236.1"
                     /translation="MDTLTHALSGALLARATAGVDAPPRSLPRRMAAGFFACAAPDLD
                     FVLGFVSPLVYLEQHRGPTHSVLLLPLWSLLFSWVLAKILREPGGWRALYGVTALGLG
                     LHILGDLITSFGTIIFAPLSYARYGWGTTFIIDLWFSGIILAGLAASLLWRRSRAPAA
                     AAALALVAYVGFQAVLKGQALDFGREYARAQGLHRAEVSAVPRAVSPFNWTVYVSDEL
                     AHRYAHVNLRRREPLPAAHDFIGRMDAAFQPLALARWETRSRYGASAAEQALARSAWA
                     SPALGFMRWFAEMPAFDGLGQGSTCVWFVDLRFVNPGREWLPFQYGACRESESAPWRA
                     FERLEGAARRALD"
     gene            8160..>8906
                     /locus_tag="VFB53_10690"
     CDS             8160..>8906
                     /locus_tag="VFB53_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87237.1"
                     /translation="MNLLRLAAIALAGLLAAPAWGTLQAPTISLAPASIVGAGTSTLT
                     ITLDNNPPGNPSNIAFTLNYPANVFNDAVPNASTNCLGGTVTAAPSGTSISLSGASLS
                     GNNICTVTVTLRSCNQAVYSIGGFGVTSTSGNPTAGTANLTVTSNSAAVAATSTVSST
                     PGSVPADDSTTSAITVTARNVCGTGAPGKSVSLAQGAGNSTITPGSAVTNASGVAAFT
                     VRNGFAETVTYTATDTTDGVVITQTAAVTFN"
BASE COUNT         1349 a         3191 c         3093 g         1273 t
ORIGIN      
        1 agccgtagtc gacgaccagc agcgcgccac gctccaggcg cgcggcgagg agcttcatcc
       61 acgcgcgcgc caccaggccg atctcgctct cgtagggcac cggcacttcg aggcgccgcg
      121 ccgcggcgag cagctcgccc gaggcaggcc gttccgccca ggcgaacttg tcgtccacgc
      181 cgcgttccat gatcgtgttg ccacgccagg cgagcgcgtg caccggcatg gcgtcggcga
      241 cttcgttcgc cagcatcacg ccgcgaaagc gttccggcag gcggtcaatc cagcgcgcgc
      301 gcttgcccag gcgcgcctcc tgccgcgcgc gcagctcggc gctggtctcg aggatgaggt
      361 agttctcggg agacagttcc ccgatcagcg cctcggcgag cgcgccgctg ccggcgccga
      421 actcgaggat cgcttcgccc ggctgcagga gcgcagccac ctggcgcgcg agcgtccgcg
      481 cgaacagcgt gcccatttcc ggcgcggtgg cgaaatcccc cggcgcgccg agcttggccg
      541 cgccgcccgc gtagtagccc agccccggct cgtggagcgc cagctccatg tagcgcgaaa
      601 acgggatcca gccgccgctg gtccgcagct cggcgcggat gcgctcgatg agcagcgcgc
      661 tggcggcgcg cgcctcggcc tccggttcgg gcagcatgcg gcgaatttaa cctagaatcc
      721 cctcgatgac ggaggcctcc ctcgagagca aggtggcgct ggtgacgggc gcggcgcggc
      781 gcgtgggcgc ggccatcgcg cgccgcctgc acgccgcggg cgcgagcgtc gtcatccact
      841 accgcggcgc cgacgccgac gcggcggcgc tggagaagga actcaacgcc gcgcgcgccg
      901 gcagcgccat gaaggtgaag ggcgacctcc tcgcccccgt ggcgcccaag gcgctggtgg
      961 acgcggcgct gcagcgcttc gcgcgcctcg acttcctcgt caacaatgcg tccaccttct
     1021 acccaaccgc gctcggccag atcgagcccg gccactggga agagctgatg ggctccaacc
     1081 tgcgcgcgcc gctgttcctc gcgcaggcgg cggcgccgca cctcgcgctc gccggcggcg
     1141 cgatcgtcaa catcgccgac atccacgccg agcggccgct gaagggctac gtcgtgtaca
     1201 gcctcgccaa ggcgggcctc gtcgcgctga cgcgctccct cgccttggag ctagcccctg
     1261 cggtacgcgt caacgccgtg gcccccggcg ccatcgcctg gcccgaggac ggccagttcg
     1321 cctcgggcga gcgcgagcgc atcatcgcca ccacgccgct cgcgcgcacc ggcgcgcccg
     1381 gcgagatcgc gcaggcggta cactttctgt gcaccgcacc cttcgtcacc ggccaggtcc
     1441 tggcggtgga cggcgggcgc tccatcttcc tgtaaccgtc ccgtccaagg gcatgcacgt
     1501 cgtcaagctg caccgcgatc cgcgcaaaga ggcatacgaa gcgggcaagc tcgccaagcg
     1561 cctgcgccgc aaggtgggcg aggcgatcgc cgacttcggc atgatcgagg caggcgacaa
     1621 ggtgatggtg tgcctctcgg gcggcaagga cagctacgcc atgctcgacg tgctgctcgc
     1681 gctgcgcgac aaggcgccgg tgcccttcga gctggtggcg gtgaacctcg accagaagca
     1741 gcccggattc cccgcgcagg tgctgccgga gtacctcgcg gggctcggcg ttccctaccg
     1801 catcgtcgag caggacacct actccgtggt gaagcgcgtc ctgcccgagg gcaagaccat
     1861 gtgctcgctc tgctcgcgcc tgcgccgcgg cgtgctgtac cgggtggcgg gggagctcgg
     1921 cgcgaccaag atcgcgctcg ggcaccaccg cgacgacatc ctggcgacgt tcttcctcaa
     1981 cctcttcttc gcggggaggt tgaaggccat gccgcccaag ctcaagtccg acgacggcag
     2041 gcacatcgtc atccgcccgc tcgcctacgt gaaggaagac gagctcgcgg cctatgcggc
     2101 ggaaaagggc ttcccgatca ttccctgcaa cctctgcggc tcgcaggagc acctgcagcg
     2161 ccgccaggtg cgcgaactcc tgcggcaatg ggaaaagcag cacgcggggc gcgtagaatc
     2221 gatcctgcgg gcgctcaccg acgtgcgcgc ctcgcacctg ctggaccgca agctgttcga
     2281 cttcgggggc ttggacgcat gaaatggatc gcaggattgc tctgcgccgc ctgcgcgctg
     2341 ccggcgcagg cgcaaccgca agagtggccg caacgcccgg tgcgcatgct ggtgggcttc
     2401 ggcgcgggcg gcggcaccga catcctggcg cgcatcgtgg cgccggcgat gggcgccgcg
     2461 ctcggccagc cggtggtggt ggagaaccgc cccggggcgg gcggctcgat cgccgccaac
     2521 gcggtggcga aggccgagcc cgacggctac accgcgttcc tgctcaacaa cgggcacgcc
     2581 gtctcggcgg tgatgctgcc ctcgctgccc tacgacccgg tggccgactt ccagcccgtg
     2641 tccatggtgg cgacgatgcc gctggtgatc ctctcgggcc cgcgcgccgg cttcggcagc
     2701 ctgcagggcc tgctcgaggt ggcgaggcac aaccccggca agctcaacct cgcctcggtg
     2761 ggcatcggca gcacgcagca cttcgccgcg gagctgctgt tccacaccgc cgcggtgaag
     2821 ctggtgcacg tcccctacaa gggaacgccc aacgcggtgg ccgccaccgt gtcgaacgag
     2881 acgcaggtgc tgatggaaac cgtgggatcg gtcatcggcc agatccgcgg gcaggcggtg
     2941 caggcgctcg cggtcacctc gagcacgcgc ttccccggcc tgccgcaggt gcccacggtg
     3001 gccgaagcgg gcctcgcgga ttacgccgtc accacctggt acgcgctggt gttcccggcg
     3061 aagacgccgg cggccgtcgt ggagaagacg cgcgccgcgc tgcgcgccgc gctcgccggc
     3121 gacgaggtgc aaaagcagat gctgaacgtg gcgttcctgc cgagcgagag cacgcccgcg
     3181 gaactcggcg cgcacctcaa ggcggaaatt gcgcgctggg gcgcggtgcg cgcgaaagcg
     3241 ggcatcaagt aggaggcggc atgaaaccgg ttgaaggcgc gagccggcag gcgcaggaac
     3301 tcgagggcat cgtgcccacc gtgaacctcg cggccgagga ggggcgcaag gatttctacg
     3361 agcgcgccaa gcggcagaac ctcgccccgc tgtggcgcgt gctgcacggc ctggtgacgg
     3421 agaagcccgc cacgcgcgcc gtgcccgcgc tgtggcgcta cgccgaggtg cggccgcacc
     3481 tgatggaggc ctgcaagctg atcggcgccg ccgaggccga gcggcgcgtg atggtgctgg
     3541 agaacccggg cctcgcgggc cagtcgcgca tcacgcagag cctgttcgcg ggcctgcaga
     3601 tcatcctgcc gggggagatc gcgccggcgc accgccatgc ggcctccgcg ctgcgcttca
     3661 tcgtcgaggg caaggacgcg ttcaccgcgg tggccggcga gcgcaccatg atggcgccgg
     3721 gggatttcgt catcaccccg tcctggacct ggcacgacca cggcaacgag ggcgcgggcc
     3781 cgatggtgtg gctcgacggc ctggacatgc acatcgtgaa cctgctctcg gcgagctttc
     3841 gcgaggacct cggcggcagg gcgcaggcgc tcgagcggcc cgagggcgcc gcgcccgccg
     3901 aattcggcaa caacctcctg ccggtggact cggtgcaccg ctcgcagacc tcgccgatct
     3961 tcaactaccc ctaccgcaag tcgcgcgagt cgctggatgc gctcgcgcgc acgcgcgcgc
     4021 cggacccctg gcacgcgcac aagatgaagt acatcaaccc ggtgaacggc ggctgggcga
     4081 tgcccaccat ctccacctgg ctgcagctga tccccaaggg cttcaggtcg gcgccctacc
     4141 gcgccaccga cagcacggtg ttcacggtgg tcgagggccg cggccgctcc accatcggtg
     4201 acgagagctt cgactggggc ccgcacgaca tcttcgtcgc gccgagctgg atggcgcacg
     4261 cgcacgaggc tgcggaagac gcggtgctgt tcctctactc cgaccgcgtg gtgcaggaaa
     4321 agctcgacct gttccgcgag gcgaaggagc ggggctgaaa cgcaggcaca tgctcggcgc
     4381 ggcgctcgcg acggtgggtc tggcgatcgc cgtggcggcc tgcgcgcgcc tgggcaacga
     4441 ctggcgcacc gccagccgcg aacccgtcgg cctggcgccc gcgccggcgg cgacccccga
     4501 ggcggtggtg caggtctatg ccgcgcgcac cgtcggtatg cgcggcctgt tcggcgtgca
     4561 cagctgggtg gcggtgaagc cggcgcgcgc ccgggcctac acggtgtacg aggtgatcgg
     4621 ctggaagctg cgctgggacg acaccgcggt atcgatccgc gagcgcgagc ccgatgcgcg
     4681 ctggttcggc aacgagcccg agctgctcgc cgaggcgcgc ggcgccgacg ccgagccgct
     4741 gatcccgaga atcgaggcgg cggtgaaaag ctatccctat cccgacgagt accgcgcctg
     4801 gccggggccc aactccaaca ccttcaccgc ctgggtgatg cgccgcgtgc ccgagctgcg
     4861 cgccgacctg ccgcccacgg cggtgggcaa ggactacctg agcggcagcc tcttcgcctc
     4921 ggcgccgagc ggcagcggca tgcagctctc gctggtcggc ctgctcggaa tcacggccag
     4981 ttcggtggag ggactggaga tcaacatcct cggcatgacc ttcggcgtgg acccctggcc
     5041 ccctgccatc aagctgccga tcgccgggcg gctcgggatg gcgagcgggc gcagcgcggg
     5101 gaacgcgaca ccctaagaac ccagtcttgt caaactggct ttgacagact agactctgcg
     5161 ccatgaattg cgcggagcag ctggtcgaga tcttcggcag ccaggccgac gtcgcccgcg
     5221 ccttccagct cgaccgcgcg gtcgtgcaca actgggtgaa gaccggctac gtgccggcgc
     5281 gctgggccgg cgaggtcgag cgcgtcaccc agggccgcat caacgcgtcg gacgtcgtca
     5341 acgaagcgag cgccaagaac ccggtgcgcg tgaagtcgcg cccggacgac gcgcccttcg
     5401 gcatcccccc ggagaaccaa gccatgagcc agtacgcccc ttccaagcgc atcacctcgt
     5461 tccatccgcc gcagcgcacg ctgatggggc ccggcccgac ggagatccac ccgcgcgtgc
     5521 tcaccaccat gagccagccg gcgatcggct acctcgaccc ggtgttcgtc gagatgatgg
     5581 aggagctgaa atcgctcctg cgctacgtct accagacgaa gaacccgctc accttcccgc
     5641 tctccggccc cggctcggta ggcatggagt tctgcttcgt caacctggtg gcgccgggcg
     5701 acaaggtgat cgtgtgccag aacggcgtat tcggcggccg gatgctcgag aacgtgatcc
     5761 gctgcggcgg cacgccgatc gtggtcgagg acaaatgggg cgagccggtg gacccgcaga
     5821 aggtggaaga cgcgctgaag aagaacaagg gcgtgggcgt gctggccttc gtgcacgccg
     5881 agacctccac cggctgccag tcggacgcgc gcgaactcgc caggctcgcg cacaagcacg
     5941 gcgcgctggt catcgtcgac gcggtgacct cgctcgccgg cacgccggtg aaggtggacg
     6001 agtgggagct cgacgccatc tactcggcga gccagaaatg cctgtcgtgc acgccgggcc
     6061 tgtcgccggt ctcgttctcc gagcgcgtgg tcgagcgcgt gaagaagcgg aaggacaaga
     6121 tccacagctg gttcatggac atgaacctgc tgctgggcta ctggaacgcg ggcgcgcgca
     6181 cctaccacca caccgcgccg acgaattcgc tcttcgcgct gcacgaggcg ctgctgctga
     6241 tccgcgagga aggcctggag aacgcctggg cgcgcgccac gcgccaccac ctcgcgctca
     6301 aggcggggct cgaggcgatg gggctgaagt tcctggtgca ggaggaatac cagctgccgc
     6361 agatgaacgc ggtgctctgc gccgagggcg tggacgaggc gcaggtgcgc tcgcgcctgc
     6421 tgtcggagtt cgggctggag atcggcgcgg ggctcgggcc gctgaagggc aagatctggc
     6481 gcttcgggct gatgggctat tcctgccgcc cggacaacgt gatgctgtgc ctctcggcgc
     6541 tcggctcggt gctcgacgac atgggctacg cggtgcacgt gggcgacgcg gaatccgcgg
     6601 cgcacggggc ctatgcccag atgcacgtca aggacgcgca gcgcaagaaa cgcgcggcct
     6661 agggcccgcc cggcatggag cattcgatcg aggcggacga ggagttcctg cgcgtgcggg
     6721 tgtccgggcg ggacacgaac accccgccgt cggaggtctg cgccgaagtg ctgcgcgaaa
     6781 gcgtccggct cgggcgcatg cgcatcctca tcgagctcga ccagaagttc ccgctgtcgc
     6841 ccgccagcca gtaccagctc gtcaccaggc tgcccgaact cggcctgatg gcgcagcatc
     6901 gcatcgcgct cgtgcaccgc aagccggagg cgcagaaggc caacgagttc atcgacgtgg
     6961 tcgcgggcaa tcgcggcatc cagatgcgca attttcccag cgtggaggag gcgaaggcct
     7021 ggctgcgcac gcaggccgcg tagccgccgc cggcgcatgg acaccctgac ccatgccctg
     7081 tcgggcgcgc tgctcgcgcg ggcgacggcg ggcgtggacg cgccgccgcg gtcgctgccg
     7141 cggcgcatgg ccgccggctt cttcgcctgc gcggcgccgg acctggattt cgtcctcggc
     7201 ttcgtctcgc cgctcgtcta cctggagcag catcgcgggc cgacgcattc ggtgctgctg
     7261 ctgcccctgt ggtcgctgct gttctcctgg gtgctggcga agatcctgcg cgagcccggc
     7321 ggctggcgcg cgctgtacgg cgtcaccgcg ctcggcctgg gcctgcacat cctcggcgac
     7381 ctgatcacca gcttcggcac catcattttc gcgccgctct cctatgcgcg ctacggctgg
     7441 ggcaccacct tcatcatcga cctgtggttc agcggcatca tcctggccgg gcttgccgcg
     7501 tcgctcctct ggcggcgctc gcgcgcgccg gcggcggcgg ccgcgctggc gctcgtcgcc
     7561 tacgtcggct tccaggcggt gctcaagggc caggcgctcg acttcggtcg cgaatacgcc
     7621 cgcgcgcagg gcctgcaccg cgcggaggtg tcggccgtgc cgcgcgcggt gtcgccgttc
     7681 aactggacgg tgtacgtgag cgacgagctg gcgcaccgct acgcgcacgt gaacctgcgg
     7741 cgccgggagc cgctgcccgc cgcgcacgat ttcatcggcc gcatggacgc cgcgttccag
     7801 ccgctggcgc tggcgcgctg ggagacgcgc agccgctatg gcgcaagcgc cgcggagcag
     7861 gcgctcgcgc gttctgcatg ggcgtcgccg gcgctcggct tcatgcgctg gttcgcagaa
     7921 atgccggcct tcgacggcct cgggcagggg tcgacttgcg tctggttcgt cgacctgcgc
     7981 ttcgtcaatc ccgggaggga atggctgccg ttccagtatg gcgcctgccg cgaaagtgaa
     8041 tccgcgccat ggcgcgcctt cgagcgcctc gagggcgccg cccgtcgggc gctggactga
     8101 gccaacattt tttcacgcct tgggcgcggc ggcggatgct aggctagccc cgtccgtcca
     8161 tgaacctcct gcgccttgcc gcaatcgccc ttgcgggcct tctcgccgcg ccggcctggg
     8221 gcaccctgca ggcgccgacg atcagcctcg caccggcatc gatcgtgggc gccggcacct
     8281 cgacgctgac catcacgctc gacaacaatc cgccgggcaa cccgagcaac atcgctttca
     8341 ctttgaacta tccggcaaac gtcttcaatg acgccgtgcc gaatgcgtcg accaactgcc
     8401 tcggcggcac tgtcaccgcc gctccatcgg ggaccagcat cagcctgagc ggcgcctcgc
     8461 tctcgggcaa taacatctgc acggtcaccg tgacactcag gtcctgcaac caggccgtct
     8521 acagcatcgg cggcttcggc gtcacctcga cctccggcaa tcccaccgcc ggcacggcaa
     8581 acctgaccgt gacatcgaat tccgcggcgg tcgcggcgac ctcgaccgta tcctccacac
     8641 caggatcggt gccggcggac gacagtacga cctccgcgat cacggtcacc gcccgcaatg
     8701 tctgcggcac cggcgcgccg gggaagtccg tcagcctggc gcagggcgcg ggcaactcca
     8761 ctatcacccc cggcagcgcg gtgacgaacg cgagcggcgt ggccgccttc accgtgagga
     8821 acggcttcgc ggagaccgtc acctacaccg ccacggatac caccgacggc gtcgtcatca
     8881 cgcagaccgc cgccgtgacg ttcaac
//
LOCUS       DASRIE010000246         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2583841, whole genome shotgun sequence.
ACCESSION   DASRIE010000246 DASRIE010000000
VERSION     DASRIE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583841"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /gene="cysC"
                     /locus_tag="VFB53_10695"
     CDS             <1..1598
                     /gene="cysC"
                     /locus_tag="VFB53_10695"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZN87238.1"
                     /translation="KNMVSGAAGADAVVLVVDAHEGVQDQTRRHAYLLRLLGIRRILV
                     AVNKMDLAGYREERYQEVRKEVAAYLRSIGVNAVDLTFLPIAAREGANIRERSGAMPW
                     YEGPTLLEALDAVPPPLAATDLPLRLTVQDVYKFDERRIIAGRIESGSLHVGDTLLFS
                     PINATARVASIESWNAEQPAKAGVGQSVGITLDEDIFVERGHVASHVHSAPVVTNVFR
                     ANLFWLGRSALVPGAQYRLRRGAAETPAVIERIDRVVDTNTLASGPGEKIEWNGVAEV
                     TVRTRGLLALDHHADNALTGRFVLVDGHQIVGGGTASTEDYPDQRRDAAPKGTNLFHV
                     RHRVAPEDRARMNGHRGGILWFTGLSGAGKSTLAVELERRLFAKGYQTFLLDGDNARQ
                     GLNADLGFSPQERAENIRRIGEVSALFAEAGMIVISAFISPYRVDRDRIRAAHGEYFN
                     EIHISAPLHVCEARDTKGLYRKARAGEVKDFTGISAPYEPPVAPELEIKSGEWTTERC
                     LAALVDYVEEHLAYRPAKIRAVV"
     gene            1598..2536
                     /locus_tag="VFB53_10700"
     CDS             1598..2536
                     /locus_tag="VFB53_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZN87239.1"
                     /translation="MHFAVFNGDADGLCALQQLHLAEGLAAAALVTGPKRRIALLAGV
                     SAGAGDQVSVLDVSFHSNRDAVLRLLAAGARVRYFDHHHAGELPEHPRLEAHIDTAPA
                     MCTSAIVDRYLGGARRAWAAVGAYGDNLKETGNALHADPRLERLGVLLNYNAYGESEQ
                     DLFFPPAELHRRLAPHADPVQFIREDPAYQTLENGYADDMQRAASLKPAAAREKAAVY
                     VLPDAPWARRVSGALANNLARSAPSRAHAMLSPNSAGGMQVSVRAPLERPAGAAGLCL
                     EYETGGGREAAAGINHLPAEKVDAFTGRFLDYFGKA"
     gene            complement(2533..3438)
                     /locus_tag="VFB53_10705"
     CDS             complement(2533..3438)
                     /locus_tag="VFB53_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN87240.1"
                     /translation="MEIPKPNLTAPAHPVLRVASWFAILVITGVTLYLAGRLFLPNNW
                     EAGMQTIAATLHSSAFWIALAIGIGAQAVDGALGMGYGVTASSFLLGTGASPAVASAS
                     THIAKLFTTGVSGIAHAKYGNVDRKLFVRLLLPGMAGGLLGVMLVTQIDGRVLKPFVS
                     AYLLVIGVYILSKAYRSVRVRPGEPRHAAKLGLFGGFVDAVGGGGWGPVVTSSLVGSG
                     NDPRTTIGSVNFAEFFMTLATAASFTFLAVEGTWPVVAGLVIGGLFAAPFAALLCKAI
                     NPRALLISVGVLITALSALTLYRSL"
     gene            complement(3510..4361)
                     /locus_tag="VFB53_10710"
     CDS             complement(3510..4361)
                     /locus_tag="VFB53_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HZN87241.1"
                     /translation="MRALIFALMLVPAVAHAQCRAIADADIIGRASAALPFAQQSGYG
                     AQVYGKHLRASAIPEDGIQGSALPNGDTVRFGKAGKALLFQLDPRDPVTSKSKRSEIS
                     FEPNIEPGKVYWIAFSVYLEDWGRLHPHDAALFGTQLHSGDNSLGLSPAFSLYTTGEG
                     RRFKVQARWSTSPTPSRHNTVSANYAERALPFGRWIDFVFRFRLNTANAGLLQVWMDG
                     ERIADHRGNLGFNTPAHRDYVKFGYYNWSPVMSSPRKVLLRSPTIVADPSGETYRAEQ
                     LRAFLGC"
     gene            complement(4363..>4852)
                     /locus_tag="VFB53_10715"
     CDS             complement(4363..>4852)
                     /locus_tag="VFB53_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87242.1"
                     /translation="YLKGLPDTHVLEPGIVLVHGGVRDVQQYMANPAHIQENARYLAA
                     DFPGARVCFFGHSHEQRVYEVKRDVDAFDVQEVAIDGQVPLRKENTYFVNPGSVDASR
                     KNRHKLAECALFDSAFSTVEFLRVRYDAAATEAKAAAFGYRIDPLTDRLYTLRRRIGA
                     RF"
BASE COUNT          740 a         1686 c         1737 g          689 t
ORIGIN      
        1 tgaagaacat ggtgagcggc gccgccggcg ccgacgcggt ggtgctggtg gtggacgcgc
       61 acgagggcgt gcaggaccag acgcggcgcc acgcctacct gctgcgcctg ctcggcatcc
      121 gtcgcatcct ggtcgcggtc aacaagatgg acctcgccgg ctaccgcgag gagcgctacc
      181 aggaggtgcg caaggaggtc gccgcctacc tgcgctcgat cggcgtcaac gcggtcgacc
      241 tcaccttcct gccgatcgcc gcgcgcgaag gcgccaacat ccgcgagcgc agcggcgcga
      301 tgccctggta cgagggcccg acgctgctcg aggcgctcga cgccgtgccg ccgccgctgg
      361 cggcgaccga cctgccgctg cgccttacgg tgcaggacgt ctacaagttc gacgagcgcc
      421 gcatcatcgc cggccgcatc gagagcggca gcctgcacgt cggcgacacg ctgctgttct
      481 cgccgatcaa cgccaccgcg cgcgtcgcct cgatcgagag ctggaacgcc gagcagcccg
      541 cgaaggcggg ggtcggccag tcggtcggca tcacgctgga cgaggacatc ttcgtcgagc
      601 gcggccacgt cgcgagccat gtgcacagcg cgccggtggt caccaacgtg ttccgcgcca
      661 acctgttctg gctcggccgc agcgcgctgg tgccgggcgc gcagtaccgc ctgcgccgcg
      721 gcgcggcgga gaccccggcg gtgatcgagc gcatcgaccg cgtggtcgac accaacacgc
      781 tcgcctccgg gccgggcgag aagatcgagt ggaacggcgt cgccgaggtc acggtgcgca
      841 cgcgcggcct gctcgcgctc gaccaccacg ccgacaacgc gctcaccggg cgcttcgtgc
      901 tggtcgacgg ccaccagatc gtcggcggcg gcaccgccag caccgaggac tatcctgacc
      961 agcggcgcga tgccgcgccc aagggcacca acctgttcca cgtgcgccac cgcgtcgcgc
     1021 ccgaggaccg ggcgcggatg aacggccacc gcggcggcat cctgtggttc accggcctct
     1081 ccggcgcggg caagtcgacg ctcgcggtcg agctggagcg gcgcctgttc gccaagggct
     1141 accagacctt cctgctcgac ggcgacaacg cgcgccaggg cctgaacgcc gacctcggct
     1201 tctcgccgca ggagcgcgcc gagaacatcc gccgcatcgg cgaggtctcg gcgctgttcg
     1261 ccgaggccgg catgatcgtg atctcggcct tcatctcgcc ctaccgggtg gaccgcgacc
     1321 gcatccgcgc cgcgcatggc gagtacttca acgagatcca catcagcgcc ccgctgcacg
     1381 tatgcgaggc gcgcgacacc aagggcctgt accgcaaggc gcgcgccggc gaggtcaagg
     1441 acttcaccgg catctcggcg ccgtacgagc cgccggtcgc gcccgagctc gagatcaaga
     1501 gcggcgagtg gaccaccgag cgctgcctcg ccgcgctggt cgactacgtc gaggagcatc
     1561 tcgcctaccg gccggcgaag atccgcgccg tggtctagtg cacttcgccg tcttcaacgg
     1621 cgacgcggac gggctctgcg ccctgcagca gctgcacctc gccgaggggc tggcggcggc
     1681 ggcgctggtc accgggccga agcggcgcat cgcgctgctc gccggcgtca gcgccggcgc
     1741 cggcgaccag gtgagcgtgc tcgacgtctc gttccattcc aatcgcgacg ccgtgctgcg
     1801 cctgctcgcg gccggcgcgc gcgtgcgcta cttcgaccac caccacgccg gcgagctgcc
     1861 cgagcacccg cggctcgaag cgcacatcga caccgcgccc gccatgtgca ccagcgccat
     1921 cgtcgaccgc tacctgggcg gcgcgcggcg cgcctgggcg gcggtgggtg cttatgggga
     1981 taacctgaag gaaaccggca acgcattgca cgccgatccc cggctggagc gcctcggcgt
     2041 tcttctcaac tacaacgcct acggcgagag cgagcaggac ctgttcttcc cgccggccga
     2101 attgcaccgc cgcctcgctc cgcatgcaga tcccgtgcag ttcatccggg aggatcctgc
     2161 ctaccagacc ctggagaacg gttacgccga cgacatgcag cgcgcggcca gcctgaagcc
     2221 cgcggcggcg cgggagaaag cggcggtcta cgtgctgccg gacgcgccct gggcgcggcg
     2281 cgtgagcggg gcgctggcga ataacctggc gcgctcggcg ccgtcgcgcg cgcacgcgat
     2341 gctgagcccg aactcggccg gcggcatgca ggtgagcgta cgcgcgccgc tcgagcgccc
     2401 ggcgggcgcg gcagggctgt gcctggagta cgagaccggc ggcgggcgcg aggccgcggc
     2461 cgggatcaat cacctgccgg cggaaaaggt cgacgccttt accggccggt ttctcgatta
     2521 cttcggaaaa gcctagaggc tgcggtagag cgtcagcgcg ctgagcgcgg tgatcagcac
     2581 gccgaccgag atcagcagcg cccgcgggtt gatcgccttg cacagcaagg cggcgaacgg
     2641 cgcggcgaac aggccgccga tcaccaggcc ggcgaccacc ggccaggtgc cctcgaccgc
     2701 gaggaaggtg aacgacgccg cggtggcgag cgtcatgaag aactcggcga agttgaccga
     2761 gccgatcgtg gtgcgcgggt cgttgcccga gccgaccagc gagctggtga ccaccgggcc
     2821 ccagccgccg ccgcccaccg cgtcgacgaa gccgccgaac agcccgagct tggccgcgtg
     2881 gcgcggctcg cccggtcgca cgcgcacgct gcggtaggcc ttgctgagga tgtaaacgcc
     2941 gatcaccagc aggtaggcgg acacgaaagg cttgagcacg cggccgtcga tctgcgtcac
     3001 cagcatgacg ccgagcaggc cgccggccat gcccggcagc agcaggcgca cgaacagctt
     3061 gcgatcgacg ttgccgtact tcgcgtgcgc gatgcccgag acgccggtgg tgaacagctt
     3121 ggcgatgtgc gtgctcgcgc tcgccacggc ggggctcgcc ccggtgccga gcaggaagct
     3181 gctcgcggtc acgccgtagc ccatgccgag cgcgccgtcg accgcctgcg cgccgatgcc
     3241 gatcgcgagc gcgatccaga aggcgctgct gtgcagggtg gcggcgatgg tctgcatccc
     3301 ggcttcccag ttgttcggca ggaacagacg ccccgccaga tagagggtga ccccggtgat
     3361 cacgaggatg gcgaaccagc tggcgacgcg caggaccggg tgagccggcg ccgtgaggtt
     3421 gggctttggg atttccatga gcccgccatc atcgccgggc tgtgttagcg ccccgccagt
     3481 cgcggtctag tccgcccggc gcgtcgccgc tagcagccga ggaatgccct gagctgctcc
     3541 gcgcggtagg tttcgccgct cgggtcggcg acgatggtcg gcgagcgcaa cagcaccttg
     3601 cgcggcgagc tcatcaccgg gctccagttg tagtagccga acttgacgta gtcgcggtgc
     3661 gcgggcgtgt tgaagccgag gttgccgcgg tgatcggcga tgcgctcgcc gtccatccag
     3721 acctgcagca agccggcgtt cgcggtgttg agcctgaagc ggaacacgaa gtcgatccag
     3781 cggccgaacg gcagcgcccg ctcggcgtag ttcgcgctga ccgtgttgtg ccggctgggc
     3841 gtcgggctcg tgctccagcg cgcctggacc ttgaagcggc ggccctcgcc ggtggtgtag
     3901 aggctgaacg ccgggctcag gccgaggctg ttgtcgcccg aatgcagctg ggtgccgaac
     3961 agtgcggcgt cgtgcgggtg gagccggccc cagtcctcca ggtacacgct gaacgcgatc
     4021 cagtagacct tgccgggctc gatgttgggc tcgaacgaga tctcggagcg cttggacttc
     4081 gaggtcaccg gatcgcgcgg gtcgagctgg aacagcagcg ccttgccggc cttgccgaag
     4141 cgcacggtgt cgccattggg aagcgcggac ccctggatgc cgtcttccgg gatcgcgctc
     4201 gcgcgcaggt gcttgccgta cacctgcgcg ccgtagccgc tctgctgcgc gaacggcagc
     4261 gcggccgagg cgcggccgat gatgtcggcg tcggcgatcg ccctgcattg ggcgtgcgcg
     4321 acggccggca ccagcatcaa ggcgaaaatc agtgcgcgca tctcagaacc tcgcgccgat
     4381 gcgccgccgc agcgtataca ggcggtcggt cagcgggtcg atgcggtagc cgaaggccgc
     4441 cgccttggct tcggtggcgg cggcgtcgta gcgcacgcgg aggaactcga cggtgctgaa
     4501 cgcgctgtcg aagagcgcgc attcggcgag cttgtggcgg ttcttgcgcg aggcgtcgac
     4561 cgatccgggg ttgacgaaat acgtgttctc cttccgcagt ggaacttggc catcgatggc
     4621 gacctcctgc acgtcgaagg cgtcgacgtc gcgttttact tcatagaccc gctgctcgtg
     4681 gctgtggccg aagaagcaga cgcgcgcgcc ggggaaatcg gcggcgaggt agcgggcgtt
     4741 ttcctggatg tgcgcgggat tcgccatgta ctgctgcacg tcgcgcacgc cgccgtgcac
     4801 caggacgatg ccgggctcga gcacatgggt gtcgggaagg cctttcaggt ac
//
LOCUS       DASRIE010000247         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3123077, whole genome shotgun sequence.
ACCESSION   DASRIE010000247 DASRIE010000000
VERSION     DASRIE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123077"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFB53_10720"
     CDS             complement(<1..904)
                     /locus_tag="VFB53_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN87243.1"
                     /translation="MTSSRMTHSSARRRWLQAGAACAATLAWPLAQGQASAPAPAAAG
                     DWPARPVTLIVPFPSGGGTDAFARPLSAALTRQLGRQVIIDNRGGAGGTVGGTIAAKS
                     APDGYTFFMGAVHHTIAPSMYPRLEYNLQTDFVPVGLVASVPQVIVVNPQRVPVNDLK
                     GLLELVRKSPGKYNYGSAGNGTSHHLAGELFKLQTKTFITHIPYRGAGPALQDLIGGQ
                     VDMMFDGLGSSSSHIKGGRIKGLAVASTRRAPGFPDLPTAAEAGVPGYEVSTWYGLWA
                     PKGTPRELVERMQAEMRTAFASSDL"
     gene            complement(943..2241)
                     /locus_tag="VFB53_10725"
     CDS             complement(943..2241)
                     /locus_tag="VFB53_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HZN87244.1"
                     /translation="MLEGLRSAARQAGHERTLRGLLTDCRRLLSERGEANSVAIARDL
                     VARFASLPADQQATFFDRLSRDFSPDPHAVLQSAQAYAKEPNARNLIRLTHLAEPPRQ
                     ELLRRINRLPGGTSSIVAMRRALLARVAQQPELQALEADFLHLLSSWFNPGFLQLQRV
                     DWNSPAQLLEQIIRHEAVHQIDGWDDLRRRLQPDRRCFAFFHPQLPAEPLIFVEVALL
                     PQMPDAIAPLIDKNSVPLRDDQFKVAAFYSISNCQPGLKGVSLGNFLIKRVAEELKRE
                     LPQLRTFCTLSPIPGFAKWVRGGAVIDGLPRVRQERLAQARAAVAQASGDDLDKWSTA
                     TALRALPEAAMEALTRLAAFYLAYHSATPAGDPVARFHLDNGARLERLNPLADLSAKG
                     VKQAFGMMVNYLYDLQKVETHHEKFTEGDIAFSRALGALL"
     gene            complement(2234..2944)
                     /locus_tag="VFB53_10730"
     CDS             complement(2234..2944)
                     /locus_tag="VFB53_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN87245.1"
                     /translation="MAEPATPLRPASLHEEVAARLRTMVFERQLEPGQWIDELALARD
                     WQISRTPLREALKVLAAEGLVTLVPRQGCRVTELSEDDAEALFPVMALLEGRCAFEAA
                     RKATPADVEALRGLHEALETHAAANNIDGYYRANHEFHCRVQALADNRWLDRATSDLR
                     KFVRLLRGRQLNWPGRVEQSIREHRTLFAAIAKGDAPRAERLMHDHLMAQLAALKALR
                     EHEKKARQRATPRSTHHA"
     gene            3101..>3635
                     /locus_tag="VFB53_10735"
     CDS             3101..>3635
                     /locus_tag="VFB53_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN87246.1"
                     /translation="MPTPQTTAQMPAAGSTPAALLPADADALLQLAAQSLFETFARTA
                     MGMLVVDRSHRIVWISDGYKRFLPALGHEESDFVGRRVEEVVPNTLMAQVIDSGQPIL
                     VDLLTNQAGTFLVSRLPLRDADGQVIGAMGLVLLDHPETTMQPLLAKFSRLQRELDAA
                     RSQLAAQRRPKYTIASFI"
BASE COUNT          499 a         1276 c         1336 g          524 t
ORIGIN      
        1 gcagatcgct gctggcgaag gccgtgcgca tctcggcctg catccgctcg accagctcgc
       61 gcggcgtgcc cttgggtgcc cacaggccgt accaggtgga cacctcgtag ccgggcacgc
      121 ccgcctcggc cgccgtgggc aggtcgggga agcccggggc gcgcctggtc gacgccaccg
      181 ccaggccctt gatgcggccg cccttgatgt gcgacgagga ggacccgagg ccgtcgaaca
      241 tcatgtccac ctggccgccg atcaggtcct gcagcgccgg ccccgcgccg cggtagggaa
      301 tgtgggtgat gaaggtcttg gtctgcagct tgaagagctc gcccgccagg tggtgcgagg
      361 tgccgttgcc ggccgagccg tagttgtact tgccggggct cttgcgcaca agctcgagca
      421 gccccttcag gtcgttgacc ggcacgcgct gcgggttcac cacgatcacc tgcggcacgc
      481 tggccacgag gccgaccggg acgaagtcgg tctggaggtt gtactcgagc cgcgggtaca
      541 tcgaaggcgc gatggtgtgg tgcaccgcgc ccatgaagaa cgtgtagccg tcgggcgcgg
      601 acttggccgc gatggtgccg cccacggtgc ctcccgcgcc gccgcggttg tcgatgatca
      661 cctgccggcc cagctgccgg gtcagcgcgg cgctcagcgg gcgcgcgaag gcgtcggtgc
      721 cgccgccgct cgggaagggc acgatcagcg tcaccggccg ggccggccag tcgccggccg
      781 ccgcgggggc cggcgcggag gcctgcccct gcgccagcgg ccacgcgagc gtggcggcgc
      841 aggcggcgcc ggcctgcagc caacgccggc gcgcggatga atgagtcatg cggctcgagg
      901 tcatcgtttt gtctccgtca ggtggatgcg ggcgctcgcc cgtcacagca gcgcgcccaa
      961 cgcgcgcgaa aaggcgatgt cgccttcggt gaacttctcg tggtgggtct cgaccttctg
     1021 caggtcgtag aggtagttga ccatcatgcc gaacgcctgc ttcaccccct tcgccgagag
     1081 gtcggcgagc gggttcagcc gctccaggcg cgcgccgttg tcgaggtgga agcgcgccac
     1141 cgggtcgccc gcgggcgtgg ccgagtggta ggcgaggtag aaggcggcca gccgcgtgag
     1201 cgcctccatc gcggcttcgg gcagggcgcg caacgcggtc gcggtggacc acttgtcgag
     1261 gtcgtcgccg ctcgcctggg cgaccgcggc gcgcgcctgc gcgaggcgct cctggcgcac
     1321 gcgcggcagg ccgtcgatca ccgcgccgcc gcgcacccac ttcgcgaagc cggggatcgg
     1381 cgacagcgtg cagaaggtgc gcagctgagg cagctcgcgc ttgagctcct cggccacgcg
     1441 cttgatgagg aagttgccca gcgacacgcc cttgaggccc ggctggcagt tgctgatcga
     1501 atagaacgcc gcgaccttga actggtcgtc acgcagcggc accgagttct tgtcgatgag
     1561 cggagcgatc gcgtcgggca tctgcggcag cagcgccacc tccacgaaga tgagcggctc
     1621 ggcgggcagc tgcgggtgga agaacgcgaa gcagcggcgg tccggctgca ggcggcggcg
     1681 caggtcgtcc cagccgtcga tctggtgcac cgcctcgtgg cggatgatct gctcgagcag
     1741 ctgcgcgggc gagttccagt ccacgcgctg gagctggagg aagccggggt tgaaccagct
     1801 cgacagcagg tgcaggaaat cggcctccag cgcctggagc tcgggctgct gcgccacgcg
     1861 cgcgagcagc gcgcgacgca tcgcgacgat gctcgacgtg ccccccggca ggcggttgat
     1921 gcggcgcagc agctcctggc gcggcggctc cgcgaggtgc gtgaggcgga tcaggttgcg
     1981 cgcgttcggc tccttggcat acgcctgcgc gctttgcagc accgcgtgcg ggtcggggct
     2041 gaagtcgcgc gagaggcggt cgaagaaggt ggcctgctgg tcggccggca gggacgcgaa
     2101 gcgcgccacc aggtcgcgcg cgatcgcgac gctgttggcc tcgccgcgtt cggacagcag
     2161 ccggcggcag tcggtcagca ggccgcgcag cgtgcgttcg tggccggcct ggcgggcggc
     2221 gctcctcaag ccctcaagca tggtgggtac tccgtggcgt ggcgcgctgg cgcgccttct
     2281 tctcgtgttc gcgcagcgcc ttcagggcgg cgagctgcgc catcaggtgg tcgtgcatca
     2341 gccgctcggc gcgcggcgca tcgcccttgg cgatcgcggc gaacagcgtg cggtgctcgc
     2401 ggatcgactg ttccacgcgg ccgggccagt tgagctggcg cccgcgcagc agccgcacga
     2461 acttgcgcag gtcgctggtg gcgcggtcca gccagcggtt gtcggcgagc gcctgcacgc
     2521 ggcagtggaa ctcgtggttc gcgcggtagt agccgtcgat gttgttggcg gccgcgtggg
     2581 tctccagcgc ctcgtgcagc ccgcgcagcg cctcgacgtc cgccggcgtg gccttgcgcg
     2641 cggcctcgaa ggcgcagcgg ccctcgagca gcgccatcac cgggaacagg gcttcggcgt
     2701 cgtcctccga cagctcggtg acgcggcagc cttgccgcgg caccagcgtg accagcccct
     2761 cggccgcgag caccttgagc gcctcgcgca gcggcgtgcg gctgatctgc cagtcgcgcg
     2821 cgagcgcgag ctcgtcgatc cactgccccg gctcgagctg ccgctcgaac accatcgtgc
     2881 gcaggcgcgc ggccacttcc tcgtgcagcg acgcggggcg caagggggtg gcgggctcgg
     2941 ccatgggcag actctataac tcataattca taattacaac ctcgggggat cccgcagtcg
     3001 ggttcccccc caaggcccgg gaaggcccgg gaaggccctc gtctgcgtcc gcaggcgccg
     3061 cgctagagtg tccgccgacc agacaggccg ccgcgaaccc atgcccaccc cgcagaccac
     3121 cgcccagatg cccgccgcgg gctcgacccc ggccgcgctc ctgccggcgg acgccgacgc
     3181 gctgctccag ctcgccgcgc aaagcctgtt cgagaccttc gcgcgcaccg cgatgggcat
     3241 gctggtggtc gaccgcagcc accgcatcgt gtggatcagc gacggctaca agcgcttcct
     3301 gcccgcgctc ggccacgagg agtcggactt cgtcggccgg cgcgtcgagg aggtggtgcc
     3361 gaacacgctg atggcgcagg tgatcgacag cggccagccg atcctcgtcg acctgctcac
     3421 caaccaggcc ggcaccttcc tggtcagccg gctgccgctg cgcgacgccg acgggcaggt
     3481 gatcggcgcg atgggcctgg tgctgctcga ccatcccgag acgacgatgc agccgctgct
     3541 cgccaagttc agccgcctgc aacgcgagct cgacgcggcg cgctcgcagc tcgccgcgca
     3601 acgccggccg aagtacacga tcgccagctt catca
//
LOCUS       DASRIE010000248         7484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2584602, whole genome shotgun sequence.
ACCESSION   DASRIE010000248 DASRIE010000000
VERSION     DASRIE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584602"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFB53_10740"
     CDS             complement(<1..876)
                     /locus_tag="VFB53_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN87247.1"
                     /translation="MAEPASERNVARFPQSRAKPRAGLVLTGGGARAAYQVGVVKAVR
                     DVLGNPVKNPFPILCGTSAGAINAATLAAHADDFTRAVAQLLEVWEHMRCELVYRTDF
                     ASIVKSGARWLSSLMLLSRHNPVSLLDNAPLREMLDKGLPFARIQEHIDSGALYAVCV
                     TASGYTSGQSVSFFQGGSGLEGWERNQRIGAAVVLQQDYLLASASLPFIFPAVKLHRE
                     YFGDGSMRQIAPVSPALHLGADRVLIVGTGRQSTSEQARARSNVYPSLAQIAGHALNS
                     IFLDSLMVDIERLERI"
     gene            complement(884..3247)
                     /locus_tag="VFB53_10745"
     CDS             complement(884..3247)
                     /locus_tag="VFB53_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZN87248.1"
                     /translation="MAAGDYTNKVAIIFPRVEEGLAVDEQLKQNALEYHRLPRPGKIS
                     ILPTKQLTNQRDLGLAYSPGVAAACEEIVRDPAQAMLLTSRANLIAVITNGTAVLGLG
                     AIGPLAAKPVMEGKSVLFKKFAGIDCFDIEVNERDPDKLVEIIASLEPTFGGINLEDI
                     KAPECFLVERKLRARMKIPVFHDDQHGTAITVGAAVLNGLKVVGKDLARVKLVASGAG
                     AAALACLDLLVKMGMPLANITVTDIQGVVYRGRKEDMDPDKERYAKDTRARKLDEVIA
                     GADVFLGLSAGGVLKSAMVQKMAERPLILALANPEPEIRPELAKAARPDCVIATGRSD
                     YPNQVNNVLCFPFIFRGALDVGATTITAEMELAAVRAIAELAQAETSEQVALAYNIES
                     ISFGPEYLIPRPFDPRLIARVAPAVAQAAMDSGVATRPIADLQAYRESLAQFVYHSGL
                     IMKPLFAAAKAKPKRVVYAEGEDERVLRAVQIVLDEGLAQPLLIGRPQVLEQRIERLG
                     LRIRAGRDFNLVNPELDPRYRDYWNSYHKLVERKGVSPEYAKVEMRRRHTLIGAMLMY
                     KGEADGMLCGTFGTHALHLHYIDQVIGLAPGVRHYAAMNALVLPNRTVFICDTYVTAD
                     PDAEHIAEMTILAAEEVRRFGLNPKVALLSASNFGSLELPSARKMQRALALLQERAPQ
                     LEVEGEMHGDAALSEEIRLKVFPGARFRGEANLLIMPGLDAANIAFNLLKTSAGDGVT
                     VGPILLGAARAAHILTPSATVRRIVNMTALTVVDANAPRAGAEPSLF"
     gene            3246..4430
                     /locus_tag="VFB53_10750"
     CDS             3246..4430
                     /locus_tag="VFB53_10750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN87249.1"
                     /translation="MTLPRMPAARRAAEIAPFQVMDVLARAQALEAAGRRVIHMEIGE
                     PDFTAPEGVVEAGVRALRAGLTAYTATLGLPALREAIAAWLARSSGRALEAARVAVTP
                     GASGALQLALALYVDPGDEILVPDPGYPGYRHFVRAFEGVARALPLAPHDAFQPTLAM
                     VRAAWGPRTRGLVLGSPSNPTGTVIAPAELARIAAFVAERGGVLIADEIYQGLVYGAP
                     PASALGLPGEVVLVSSFSKYFCMTGWRLGWAVLPAHRVREFEKLAQHFYICAPSAAQH
                     AALAAFAPEALQVLEARRLEFQRRRDFLVPALERAGLTLPARPTGAFYAYADCAAFGG
                     DAKRFALQLLEDAGVAATPGADFGANGTASCLRFAYTRSLDDLEEGAARIAAYCAARA
                     AG"
     gene            complement(4427..4891)
                     /locus_tag="VFB53_10755"
     CDS             complement(4427..4891)
                     /locus_tag="VFB53_10755"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HZN87250.1"
                     /translation="MGLPEEARLIFLSAASRLRALLGAVLLGLACAAPAQPLGGEEVR
                     AMRLVIDAQLEAFARDDSEGAFSLASPGIRERFVTAETFVRMVREQYAVVYRSASRTF
                     LKPVVEDGTVIFPVRLSDRDGREWLALYTMQRVAGEWRVAGCQLVPGAALAT"
     gene            complement(4904..5572)
                     /locus_tag="VFB53_10760"
     CDS             complement(4904..5572)
                     /locus_tag="VFB53_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZN87251.1"
                     /translation="MLAIEKMTRAFSVARGTLALIGLAVVLAAVLPLPREALIRHVAA
                     AAVPEGGDPASVQAAGTILPAGAASMQEREQRAVAEFIARRYRVAETASSAFVSTAYR
                     AGQEHAVDPLLILAVMAIESRYNPVAESSMGAKGLMQIIPRYHQEKLLEHGGEFAVLD
                     PEVNILVGAQILREYMRRFGATETALQMYGGAMDEPTAQYAGKVLAEKARLKQFLAQA
                     LRRA"
     gene            complement(5998..6204)
                     /locus_tag="VFB53_10765"
                     /pseudo
     CDS             complement(5998..6204)
                     /locus_tag="VFB53_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
     gene            complement(6398..6646)
                     /locus_tag="VFB53_10770"
     CDS             complement(6398..6646)
                     /locus_tag="VFB53_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZN87252.1"
                     /translation="MHRVTACKVEEDYKLWLRFDDGLEGSVFLGNLLEIGAFKLWRDV
                     REFEKVSVDPATATVTWEGGIRLDPDILYQDLAARSAR"
     gene            complement(6657..>7484)
                     /locus_tag="VFB53_10775"
     CDS             complement(6657..>7484)
                     /locus_tag="VFB53_10775"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZN87253.1"
                     /translation="ERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQA
                     FEEAIHTHAYQYIVESLNLDEGEVFNAYNEIASIRAKDEFLVPFIDTLTDPHFKTGTP
                     DKDQDLLRSLIVFSCLMEGLFFYVGFTQILALGRQNKMTGAAEQYMYILRDESMHCNF
                     GIDLVNQIKLENPHLWTPQFRDELQELFRQGVELEYRYAEDTMPRGVLGLNAPMFKEY
                     LRYIANRRCQQIGLDALYPGASNPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWE"
BASE COUNT         1037 a         2627 c         2590 g         1230 t
ORIGIN      
        1 tatgcgctcc aggcgctcga tgtccaccat caggctgtcg aggaagatgg aattgagcgc
       61 atggccggcg atctgcgcca gcgacgggta gacgttggag cgcgcgcgcg cctgctcgga
      121 ggtgctctgg cggcccgtgc cgacgatcag cacgcggtcg gcgcccaggt gcagcgccgg
      181 gctcaccggc gcgatctggc gcatcgagcc gtcgccgaag tactcgcggt gcagcttcac
      241 cgccgggaag atgaacggca gcgaggccga ggcgagcagg tagtcctgct gcagcaccac
      301 cgcggcgccg atgcgctggt tgcgctccca gccctccagc ccggaaccgc cctggaagaa
      361 cgacacgctc tgcccgctgg tgtagcccga ggcggtgacg cacaccgcgt acagcgcgcc
      421 cgagtcgatg tgctcctgga tgcgcgcgaa cggcaggccc ttgtcgagca tctcgcgcag
      481 cggcgcgttg tcgagcagcg ataccgggtt gtggcgcgac agcagcatca gcgaggacag
      541 ccagcgcgcc ccgctcttca cgatgctggc gaagtcggtg cggtacacca gctcgcagcg
      601 catgtgctcc cacacctcga gcagctgcgc cacggcgcgc gtgaaatcgt cggcgtgcgc
      661 cgccagcgtc gccgcgttga tcgcgcccgc cgaggtgccg cacaggatcg ggaacgggtt
      721 cttcaccggg ttgccgagca cgtcgcgcac cgccttcacc acgcccacct ggtaggcggc
      781 gcgcgccccg ccgccggtca gcaccagccc ggcgcgcggc ttcgcccggg actgcgggaa
      841 acgcgcgacg ttgcgctcgc tcgccggttc ggccatcggc gtcctagaac aggctcggct
      901 ccgcgccggc gcgcggcgcg ttggcgtcga ccacggtgag cgcggtcatg ttgacgatgc
      961 gccgcacggt ggccgagggg gtgaggatgt gcgcggcgcg cgccgcgccg agcaggatcg
     1021 ggccgacggt gacgccgtcg ccggccgagg tcttgagcag gttgaaggcg atgttggcgg
     1081 cgtccagccc gggcatgatc agcaggttgg cctcgccgcg gaagcgcgcg ccggggaaca
     1141 ccttcagccg gatctcctcc gacagcgccg cgtcgccgtg catctcgccc tccacctcca
     1201 gctgcggcgc gcgctcctgc agcagggcga gcgcgcgctg catcttgcgc gccgagggca
     1261 gctcgagcga gccgaagttg gaggccgaca gcagcgccac cttggggttc aggccgaagc
     1321 ggcgcacctc ttcggcggcg aggatggtca tctcggcgat gtgctcggcg tcggggtcgg
     1381 cggtgacgta ggtgtcgcag atgaacaccg tgcggttggg cagcaccagc gcgttcatcg
     1441 ccgcgtagtg gcgcacgccg ggcgccaggc cgatcacctg gtcgatgtag tgcaggtgca
     1501 gcgcgtgcgt gccgaaggtg ccgcacagca tgccgtcggc ctcgcccttg tacatcagca
     1561 tcgcgccgat cagcgtgtgc cgccggcgca tctccacctt ggcgtactcg ggcgacacgc
     1621 ccttcctttc caccagcttg tggtagctgt tccagtagtc gcggtagcgc gggtcgagct
     1681 cggggttcac caggttgaag tcgcggcccg cccggatgcg caggccgagc cgctcgatgc
     1741 gctgctcgag cacctgcggg cggccgatga gcagcggctg ggcgagcccc tcgtccagca
     1801 cgatctgcac cgcgcgcagc acgcgctcgt cctcgccctc ggcgtacacc acgcgcttgg
     1861 gcttcgcctt ggccgcggcg aacagcggct tcatgatcag cccggagtgg tagacgaact
     1921 gcgccagcga ctcgcggtag gcctgcaggt cggcgatcgg gcgcgtcgcc acgccggaat
     1981 ccatcgccgc ctgcgccacc gcgggcgcca cgcgcgcgat caggcgcggg tcgaagggcc
     2041 gcgggatcag gtactccggg ccgaagctga tcgattcgat gttgtaggcg agcgccacct
     2101 gctcggaggt ctcggcctgg gcgagctcgg cgatggcgcg caccgccgcc agctccatct
     2161 cggcggtgat ggtggtggcg ccgacgtcga gcgcgccgcg gaagatgaac ggaaagcaca
     2221 gcacgttgtt cacctggttc gggtagtccg agcgcccggt ggcgatgacg cagtcggggc
     2281 gcgccgcctt cgccagttcc gggcggatct ccggctccgg gttggcgagc gcgaggatca
     2341 gcgggcgctc ggccatcttc tgcaccatcg cgctcttcag cacgccgccg gccgacaggc
     2401 cgaggaacac gtcggcgccc gcgatcacct cgtcgagctt gcgcgccctg gtgtccttcg
     2461 cgtagcgctc cttgtccgga tccatgtcct ccttgcggcc ccggtacacc acgccctgga
     2521 tgtcggtgac ggtgatgttg gcgagcggca tgcccatctt caccagcagg tcgaggcagg
     2581 cgagcgccgc ggcgccggcg cccgaggcca ccagcttcac ccgcgccagg tccttgccga
     2641 ccaccttcag gccgttgagc acggcggcgc ccacggtgat ggcggtgccg tgctggtcgt
     2701 cgtggaacac cgggatcttc atgcgcgcgc gcagcttcct ctccaccagg aagcattccg
     2761 gcgccttgat gtcctccagg ttgatgccgc cgaaggtcgg ctccaggctg gcgatgatct
     2821 ccaccagctt gtcggggtcg cgctcgttca cctcgatgtc gaagcagtcg atgccggcga
     2881 acttcttgaa cagcacgctc ttgccttcca tcaccggctt ggcggcgagc ggcccgatgg
     2941 cgcccaggcc gagcaccgcg gtgccgttgg tgatcaccgc gatcaggttg gcgcgcgagg
     3001 tgagcagcat cgcctgcgcc gggtcgcgca cgatctcctc gcaggccgcg gccacgccgg
     3061 gcgaataggc gaggcccagg tcgcgctggt tggtgagctg cttggtggga aggatcgaga
     3121 tcttcccggg gcgcggcagg cggtgatatt cgagtgcgtt ctgcttgagc tgctcgtcca
     3181 ccgccaaccc ctcctcgacc cggggaaaaa ttatagcaac cttgttcgta taatcccccg
     3241 cagccatgac cctgccccgc atgccggcgg cgcgccgcgc cgccgagatc gcgccgttcc
     3301 aggtgatgga cgtgctcgcg cgggcgcagg cgctcgaggc cgcgggacgg cgcgtgatcc
     3361 acatggagat cggcgagccg gacttcaccg cgcctgaagg cgtggtggag gccggcgtgc
     3421 gcgcgctgcg cgccggcctc accgcctaca ccgcgaccct cggcctgccc gcgttgcgcg
     3481 aggcgatcgc cgcgtggctg gcgcgctcct ccggccgggc gctggaggcg gcgcgcgtcg
     3541 ccgtcacgcc gggcgcctcg ggcgctctgc agctcgcgct cgcgctgtac gtcgacccgg
     3601 gcgacgagat cctggtgccg gacccgggct atccgggcta ccggcatttc gtgcgcgcct
     3661 tcgagggcgt ggcgcgcgcg ctgccgctcg cgccgcacga cgccttccag ccgacgctcg
     3721 ccatggtgcg cgcggcctgg ggaccgcgca cgcggggcct ggtgctcggc tcgccctcca
     3781 atccgaccgg cacggtgatc gcgccggcgg agctcgcgcg catcgcggcg ttcgtcgccg
     3841 agcgcggcgg cgtgctgatc gccgacgaga tctaccaggg cctggtgtac ggcgcgccgc
     3901 cggcgagcgc gctcggtctg ccgggagaag tggtgctggt gagcagcttc tccaagtatt
     3961 tctgcatgac cggctggcgc ctgggctggg cggtgctgcc ggcgcaccgg gtgcgcgagt
     4021 tcgagaagct ggcgcagcac ttctacatct gcgcgccgag cgccgcgcag cacgccgcgc
     4081 tcgccgcctt cgcgccggag gcgctgcagg tgctggaggc gcggcgcctg gagttccagc
     4141 ggcggcgcga tttcctggtg ccggcgctgg agcgcgcggg cctcacgctg ccggcgcgcc
     4201 ccacgggcgc gttctacgcc tacgcggatt gcgcggcctt cggcggcgac gcgaagcgct
     4261 tcgcgctgca gctgctcgag gacgccgggg tcgccgcgac gccgggcgcg gactttggcg
     4321 ccaacggcac cgcgagctgc ctgcgcttcg cctacacgcg cagcctggac gatctcgagg
     4381 agggcgccgc gcgcatcgcc gcctactgcg ccgcgcgcgc cgccggctag gtggcgagcg
     4441 ccgcccccgg caccagctgg cagccggcga cgcgccactc cccggccacg cgctgcatgg
     4501 tgtagagcgc cagccactcg cggccgtcgc ggtcgctcag ccgcaccgga aagatcacgg
     4561 tgccgtcctc gaccaccggc ttgaggaagg tgcgcgaggc cgagcggtac accaccgcgt
     4621 actgctcgcg caccatgcgc acgaaggtct cggcggtgac gaagcgctcg cggatgccgg
     4681 gcgaggcgag cgagaacgcg ccttcggaat cgtcgcgcgc gaaggcttcc agctgcgcgt
     4741 cgatcaccag gcgcatcgcc cgcacctcct cgccgccgag cggctgcgcc ggcgcggcgc
     4801 aggccagccc cagcagcacc gcgcccagca gggcgcgcag gcgggacgcg gcggacagga
     4861 agatcaggcg ggcctcctcc gggaggccca ctctagcagg ctctcaggcc cggcgcagcg
     4921 cctgggcgag gaactgcttc aggcgcgcct tctccgccag caccttgccg gcgtactgcg
     4981 cggtgggctc gtccatggcg ccgccgtaca tctgcagcgc ggtctcggtg gcgccaaagc
     5041 gccgcatgta ctcgcgcagg atctgggcgc cgaccaggat gttcacctcg gggtcgagca
     5101 cggcgaactc gccgccgtgc tccaggagct tttcctggtg gtacctgggg atgatctgca
     5161 tcaggccctt ggcgcccatc gagctctcgg ccaccgggtt gtagcgcgac tcgatcgcca
     5221 tcaccgcgag gatcagcagc gggtccaccg cgtgctcctg ccccgcccgg taggcggtcg
     5281 acacgaacgc cgagctggcc gtctccgcga cgcggtagcg ccgcgcgatg aattccgcca
     5341 ccgcgcgctg ttcgcgctcc tgcatcgacg ccgcgccggc cggcaggatg gtcccggccg
     5401 cctgcaccga cgccgggtcg ccgccttcgg gcaccgccgc cgcggccacg tgccggatca
     5461 gcgcctcgcg gggcagcggc agcaccgccg ccagcaccac cgccaggccg atcagggcca
     5521 gcgtgccgcg cgccaccgag aacgcccgtg tcatcttttc gattgccagc atcgtccatc
     5581 tcctcgcacc aggcgccgcc tgcccgccac ccggcctgga gcggccccgg ctggaggccg
     5641 ggtgtcgcga ggaagcaggc ttgggacgaa tgccggagga cccagagggg gagtccggcc
     5701 gcagtgcaga ttgctgcggt gcagtgtcga ccatgctatg ccgaattagc caatgtcgtc
     5761 aatgccttct gacatttgat gatcctccgt tcggaccatg tccaccgccg acccgcgggc
     5821 cggcgaacct gccctgcgag gcgctttcca gaccggtaga atgccggctc gatccaggga
     5881 agcgcgcgga ccgacctcag cctttgagtg acgccccaac gacggaggcg gtccgcagtg
     5941 cttcgagggc ctggcggggg gcttgcgccc cccgccatat tcctggtcaa cccccggtta
     6001 cggccgggag ccggtaggaa acggccaagc ggccgccgga ttgagcgcgg ttcgcgcgcc
     6061 agttgtcgcg gcggtcgacg gcggtgttgc acttgccgcc gtcgtcatac ccgcagggcc
     6121 ggccataggt ttaacgggct tcgccttttt tttcttggca gatcttttct ttcggcccgc
     6181 tgccttcttc ttggaagact tcttaactgc tttcttcttc ttttttgatg atttcttctt
     6241 cttctttttc tttccgcctt ttttcgcttt tttcttcgcc atgactagct ccttactggt
     6301 agttggacaa aagaagcccg gtcatctctg accggactat tcaaccgccg ccggggatct
     6361 ctcccctgcg gcggactgaa ttcttcgcaa cggcccatca gcgcgcgctc cgcgccgcga
     6421 gatcctggta caggatgtcc ggatcgagcc ggatgccgcc ttcccaggtc actgtcgcgg
     6481 ttgccggatc gacgctcacc ttctcgaact ctcgcacatc gcgccacagc ttgaaagcgc
     6541 cgatctcgag caggttgccg aggaatacgc tgccttccag gccgtcgtcg aagcgcagcc
     6601 acagcttgta atcctcttcc accttgcagg ccgtcacgcg gtgcatcctg ttctcctcat
     6661 tcccagctca gcgcgccgcc ggtctggtat tcgatcaccc gcgtctcgaa gaagttgcgt
     6721 tccttcttca ggtcgatcat ctcgctcatc caggggaagg ggttgctcgc ccccgggtac
     6781 agcgcgtcca ggccgatctg ctggcagcgg cggttggcga tgtagcgcag gtactccttg
     6841 aacatcggcg cgttcagccc gagcacgccg cgcggcatgg tgtcctcggc gtagcggtac
     6901 tcgagctcca ccccctggcg gaacagctcc tgcagctcgt cgcggaactg cggcgtccac
     6961 aggtgcgggt tctccagctt gatctggttc accaggtcga tgccgaaatt gcagtgcatc
     7021 gactcgtcgc gcaggatgta catgtactgc tcggccgcgc cggtcatctt gttctgccgg
     7081 ccgagcgcca ggatctgcgt gaagccgacg tagaagaaca gtccttccat caggcaggag
     7141 aacacgatca ggctgcgcag caggtcctgg tccttgtccg gcgtgccggt cttgaagtgc
     7201 gggtcggtga gggtgtcgat gaacggcacc aggaactcgt ccttggcgcg gatcgaggcg
     7261 atctcgttgt aggcgttgaa cacctcgccc tcgtcgaggt tcaggctctc gacgatgtac
     7321 tggtaggcgt gggtgtggat cgcctcctcg aacgcctgcc gcagcaggta ctggcggcac
     7381 tcgggcgccg tgatgtgccg gtaggtgccc agcacgatgt tgttggcggc gagcgagtcc
     7441 gcggtgacga agaagccgag gttacgcttc acgatgcgcc gctc
//
LOCUS       DASRIE010000249        14446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2325845, whole genome shotgun sequence.
ACCESSION   DASRIE010000249 DASRIE010000000
VERSION     DASRIE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325845"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /gene="fusA"
                     /locus_tag="VFB53_10780"
     CDS             complement(<1..1952)
                     /gene="fusA"
                     /locus_tag="VFB53_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN87254.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDLSYPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYDAVAGVQPQSETVWRQANKYKVPRLAFINKMDRVGATFFKSYEHIKN
                     RLKGNPVPIQIPIGAEEKFEGVVDLVTMQAIYWDEASQGMKFERRAVPAELAAEAKEW
                     REKMVEAAAEANEELMNKYLESGELSIEEMKRGLRLRTINNEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDIPPVKGSKENGEPDVRKPEDEEPFAALAFKIMTDPFVGQLSFI
                     RVYSGVLASGDTVHTSVRGRKERIGRLLQMHANERQEIKEVRAGDIAAVVGLKDVITG
                     ETICDAKKIIVLERMEFPDPVISQAVEPKTKVDQEKMGVALGRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEASVGKPQVAYRETFKKSLEQEGKFIKQSGG
                     RGQYGHVWLKLEPNHSKGYEFVDGIKGGRVPREFIPAVKKGVDEALQDGVLAGYPVVD
                     VKVTLFDGSYHEVDSNENAFRMAASMAFKDGMRRAQPILLEPMMAVEVETPEEKMGDV
                     MGDLSARRGMIQGMEDLGGGKAIRAEV"
     gene            complement(1972..2442)
                     /gene="rpsG"
                     /locus_tag="VFB53_10785"
     CDS             complement(1972..2442)
                     /gene="rpsG"
                     /locus_tag="VFB53_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZN87255.1"
                     /translation="MPRRREVPKREILPDPKFNHPEVAKFINVLMTRGKKSVAEGIVY
                     RAFDVIKTKSGKDPIEVFTQAVQNVKPVVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WLREAAQKRGEKSMTVRLAGELQEAAEGRGGAMKKREEVHRMADANKAFAHYRF"
     gene            complement(2460..2837)
                     /gene="rpsL"
                     /locus_tag="VFB53_10790"
     CDS             complement(2460..2837)
                     /gene="rpsL"
                     /locus_tag="VFB53_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZN87256.1"
                     /translation="MPTVNQLVRFGRSTKRAKSKVPALRQSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDMPGVRYHIVRGSL
                     DTQGVKDRKQSRSKYGAKAPKSA"
     gene            complement(2916..3419)
                     /locus_tag="VFB53_10795"
     CDS             complement(2916..3419)
                     /locus_tag="VFB53_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HZN87257.1"
                     /translation="MDPQVLGSLAKWPDVPAVYGWLALDRRGNWLLRETADGRFGRIG
                     NAALREFISRNYAPDERGGWYFQNGPQRVYVRLAYAPLVLHYEGNSLFDHCARPAAAL
                     ETYLDDEGSVLMLTSRGLGLLDDRDLARYADAAAPGARLRRGEAEARFGFVSEPRFPC
                     AGPASSL"
     gene            complement(3422..4195)
                     /locus_tag="VFB53_10800"
     CDS             complement(3422..4195)
                     /locus_tag="VFB53_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN87258.1"
                     /translation="MSQELTGWRDPSARLRQALDLDELQLYCQPILSLHPPGGFAMAE
                     VLVRLREEETLMLPPGDFLPVFEHYGMMPELDRWVVSHVLRWLAGGRPGGFRSFSVNV
                     SVQTIGDQDFPAFVAAELRSTRIAPAALTFEIEEASVLARTASAAGFAAAMKAAGCKV
                     AIDGFGQRSVSFAPLKSLRVDYVKVDGSIVRNLLRSGVAEQKLKAIVRVGEAIGVGVI
                     AECVEDADILARLKLLNVGFAQGFGIARPAPIAQRPAGQ"
     gene            4338..4775
                     /locus_tag="VFB53_10805"
     CDS             4338..4775
                     /locus_tag="VFB53_10805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN87259.1"
                     /translation="MAREDFACTHRLRVRWAEVDMQKIVFNGHYLTYIDTAVADYWRA
                     IGLPYPEGYVERFGSDMFLRKATVEYLGSAHYDDALEVLCRTAKLGRTSMTFLFEIWR
                     GGDLLIGAELVYVNADPRSRKAVPLPEELRRCVLGFEKVAPVQ"
     gene            4772..5221
                     /locus_tag="VFB53_10810"
     CDS             4772..5221
                     /locus_tag="VFB53_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN87260.1"
                     /translation="MTLYWEDFKVGDCVEMGSHRFSEEEIIAFARQFDPQPFHIDPVA
                     AKSSAFGGLIASGWHTCAVCMRLNCDSYINRTVSLGSPGLDNIRWLKPVRPGDTIRFR
                     RTVLDARASLSRPGVGLLRSRTEALNQHGVVVMSMEGWGMFGRRPAA"
     gene            5218..5994
                     /locus_tag="VFB53_10815"
     CDS             5218..5994
                     /locus_tag="VFB53_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN87261.1"
                     /translation="MSAFTVAALAVYPVKGAAAVALQAFEVQDCGPDADRRYCLVRPG
                     GRVFTQRDDPGLARLRAALRGEHLSLEFDGEQLAVGPGAFVATAEIRVWSRSATARVA
                     DAGTNACVSQWFGTPLLLAKLEHPLQREGAALAFGDAAALLVANLASLDALNAALLAP
                     VPMDRFRPNIIVGGAAAYAEDGWRKLRVGGALLAPVHPCGRCQVTTIDQEFGAVLGDE
                     PLRTLARLRTRDGEAVFGVRYAVERPGRVRVGDRVEPLDQ"
     gene            complement(5988..7136)
                     /gene="clsB"
                     /locus_tag="VFB53_10820"
     CDS             complement(5988..7136)
                     /gene="clsB"
                     /locus_tag="VFB53_10820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZN87262.1"
                     /translation="MHYVEGNRLLLLRNGEQYFPALVEAIEGARREVFVESYIYADDQ
                     TGSLVTDALARAAARGASVHLLVDGFGSRGFAPRFRRMLARAGARFLVYRPNISPWTL
                     RRDRLRRMHRKLACVDGRVAFVGGINIVDDYDGPDPAPPRADYAVRIEGPLTATVRAE
                     AAALWERVAGRSGRATQAQAPAPLGSFPDGTRAALVVRDSVRHRRDIERAYLASIDAA
                     REEVVIANAYFFPGRRFRQALLRAAHRGVRVVLLLQGRVEYLLQHYASRALYGALLDA
                     GIEIHEYRRGFLHAKVAVFDCCRASVGSTNIDPFSLLLAREANVFVDDARFAAGLRAS
                     LEEAMRVGASRLAALQWKRQPLWERVLTWCGYGIARLLISFAGYERYH"
     gene            complement(7141..7890)
                     /locus_tag="VFB53_10825"
     CDS             complement(7141..7890)
                     /locus_tag="VFB53_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN87263.1"
                     /translation="MTESLSVVTLNIHKGLSQFNRRVVIHELREGLRTLAPDLVFLQE
                     VQGLNERHAVRFPSWPNAPQHEFLAPADWQHAYGRNRVHHHGHYGNAILSRFRILSTE
                     NEDVSDHRFERRGVLHCVVAVPGWRRNLHCVCVHLSLHEGGRRRQLEAVSARLDALAG
                     RDLPIVLAGDFNDWRQRATRVLAQRLGMTEVTSAYGRHAARTFPSLLPLLRLDRIYVR
                     GFSVVEARVHRGPPWSLLSDHLALGARLKPH"
     gene            complement(7887..8336)
                     /gene="nudB"
                     /locus_tag="VFB53_10830"
     CDS             complement(7887..8336)
                     /gene="nudB"
                     /locus_tag="VFB53_10830"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZN87264.1"
                     /translation="MRAKLPVSVLVVIHTAALDVLLLERASRPGYWQSVTGSLDRLDE
                     PLQDAARRELREETGIDADGPALVPWHLANAFEIYAHWRRRFAPGTTHNTEHVFGLML
                     PQRIAVTLAAQEHRAQQWLPWREAAEKCFSWSNRDAIRMLPARSAAA"
     gene            complement(8336..8767)
                     /locus_tag="VFB53_10835"
     CDS             complement(8336..8767)
                     /locus_tag="VFB53_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZN87265.1"
                     /translation="MGKLTQRLTDAARSGVYRAPRADEVLDGARGTRLDLAGVDLGGA
                     TDRESLLERLAAALAFPAWFGGNWDALEDCLADLSWRAGDGHVVLIGGHGELPSDDLG
                     VLVDVLRAAAEHWAGRGKPFFAVFIDPGNAMALPALFRPKA"
     gene            complement(8771..9076)
                     /locus_tag="VFB53_10840"
     CDS             complement(8771..9076)
                     /locus_tag="VFB53_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZN87266.1"
                     /translation="MVLAFAANAWAGDIPLAGLPPEARATLALIRAGGPFPHQQDGRA
                     FGNREKLLPRRARGYYREYTVTTPGARDRGARRIVAGKDGEFYYTADHYRTFQRIRE"
     gene            complement(9091..10863)
                     /gene="aspS"
                     /locus_tag="VFB53_10845"
     CDS             complement(9091..10863)
                     /gene="aspS"
                     /locus_tag="VFB53_10845"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZN87267.1"
                     /translation="MRTHYCADLSAALIGQKVTLAGWAHRRRDHGGVIFIDLRDRAGL
                     AQVVCDPDRAEAFSAADRVRGEFVLRVTGLVRARPAGTTNPNLKSGEIEVLAQEVEIL
                     NPAVPPPFQLDEGNLSEQVRLEHRVVDLRREQMQHNLRLRHAAARAARGFLDANGFVD
                     IETPVLYKSTPEGAREFLVPSRLHDGQFYALPQSPQLFKQLLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLGEQEIRALMERLARHMFGEVLGVALPDPFPVLSYDE
                     AMRDYGVDKPDLRVPLKLAELTDVVRDVDFKVFSAPAGMPGGRVAGLRVPGGGALTRG
                     EIDAYTEHVKALGARGLAWIKVNARAKGADGLQSPIVKNLHARAIAAILERTGAADGD
                     LVFFAADKAETANACLGALRQKIGHERGLAAQGWRPLWVVDFPMFEYDEESRQWKARH
                     HPFTSPKDGHEQYLESAPEKAYAKAYDMVLNGWEVAGGSVRIHRQELQQRVFRGLKIS
                     AEDQRAKFGFLLDALQYGAPPHGGIAFGFDRLVALMVGADAIRDVIAFPKTQRGQDLL
                     TGAPSAVSEKQLRELHIRLRNAPQ"
     gene            complement(10871..11470)
                     /locus_tag="VFB53_10850"
     CDS             complement(10871..11470)
                     /locus_tag="VFB53_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZN87268.1"
                     /translation="MKKYLITGLLIWIPLVITLWVLKLIVDTLDQSLLLLPEQWRTEG
                     FLGVHVPGLGVILTLVIVVATGVFATNFFGARLVQLGHDLLHRIPVVNSIYSSVKQIS
                     DTLFSSSGQAFRKALLVQWPREGMWTIAFLTGTPGGDVATHLQGDFVSVYVPTTPNPT
                     SGFFVMLARADVIELDMSVDAALKYIISMGVVAPGQGGR"
     gene            complement(11531..11863)
                     /locus_tag="VFB53_10855"
     CDS             complement(11531..11863)
                     /locus_tag="VFB53_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZN87269.1"
                     /translation="MPIYEYRCAACGFQDEFLQKVSERPLKVCPSCGKAKFEKMLSAP
                     GFQLKGSGWYATDFKGGAKPAAEKADAAGEAKSEAKAEPKADSKTEAKAEPAKPAKAN
                     KAPATTTN"
     gene            complement(11917..12378)
                     /gene="ssb"
                     /locus_tag="VFB53_10860"
     CDS             complement(11917..12378)
                     /gene="ssb"
                     /locus_tag="VFB53_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZN87270.1"
                     /translation="MASVNKVIVVGNLGADPETRYLPSGEAVTNIRVATTDRWKDKAS
                     GEMKEATEWHRIAFFGRLAEVAGEYLKKGSQVYVEGSLRTRKWQDKEGHDRYSTEIRA
                     DVMQMLGSRAGAGEPRGAPAGGGASEPASEPKSGAKKPAGKFDDMADDIPF"
     gene            12509..>14446
                     /gene="uvrA"
                     /locus_tag="VFB53_10865"
     CDS             12509..>14446
                     /gene="uvrA"
                     /locus_tag="VFB53_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN87271.1"
                     /translation="MDSSPTSTIRIRGARTHNLKNISVDLPRHRLVVITGLSGSGKSS
                     LAFDTLYAEGQRRYVESLSAYARQFLQMTEKPDVDLIEGLSPSIAIEQKAGSHNPRST
                     VGTITEIHDYLRLLYARVGTPYCPDHDLPLQAQSVSQMVDQVLSLPPDTRLMVLAPVV
                     AGRKGEQADLLEELRAQGFTRVRVDGKVLELEAAPRLAKNLKHSVDVVVDRLKVRAES
                     KQRLAESFETALRHADGRAIAVEADSGKEHQFSARFSCPACAYTLPELEPRLFSFNNP
                     MGACPRCDGLGSVEFFDPRRVVAHPNLSLAGGAIRGWDRRNHFYFTMLQALARRYAFD
                     VEAPWEALADEAQQLILYGSGKEKIAFSYPGERGRTAVREHAFEGVVPNLERRYRDTD
                     SAVVKEELAKYRNRRTCPECGGTRLRREARHVRVADRNLHAVSCLPLRQALELFRGLG
                     ATLQGARAAVGERIVKEIADRLEFLVNVGLDYLSLERSAETLSGGEAQRIRLASQIGS
                     GLTGVMYVLDEPSIGLHQRDNARLIETLKRLRDLGNSVIVVEHDEEAIRAADYVLDMG
                     PGAGEAGGHVVAQGRPEDVIADPQSLTGRYLAGTLQIDVPRLRNRPGKNRLVVRGASG
                     HNLKEVDLELPLGLLVCVTGVS"
BASE COUNT         2125 a         5169 c         4871 g         2281 t
ORIGIN      
        1 acctcggcgc ggatcgcctt gccgccgccg agatcttcca tgccctggat catgccgcgc
       61 cgtgcggaga gatcgcccat cacgtcgccc atcttctcct cgggcgtctc cacctcgacc
      121 gccatcatcg gctcgagcag gatcggctgc gcgcggcgca tcccgtcctt gaacgccatc
      181 gacgctgcca tccggaacgc attctcgttc gagtcgacct cgtggtacga gccgtcgaac
      241 agcgtgacct tcacgtccac caccgggtaa ccggcgagca cgccgtcctg cagcgcctcg
      301 tccaccccct tcttcaccgc cgggatgaat tcgcgcggca cgcggccgcc cttgatgccg
      361 tcgacgaact cgtagccctt gctgtggttc ggctcgagct tcagccagac gtggccgtac
      421 tggccgcgcc cgccggactg cttgatgaac ttgccttcct gctcgagcga cttcttgaag
      481 gtctcgcggt aggccacctg cggcttgccg accgaggcct cgacgccgaa ctcgcgcttc
      541 atgcggtcga cgatgatctc caggtgcagc tcgcccatgc ccgagatgat ggtctggccc
      601 gactcctcgt cggtgcgcac gcggaacgac gggtcctcct gcgccaggcg cccgagcgcc
      661 acgcccatct tttcctggtc gaccttggtc ttcggctcga cggcctgcga gatcaccggg
      721 tccgggaact ccatgcgctc gagcacgatg atcttcttcg cgtcgcagat cgtctcgccg
      781 gtgatcacgt ccttcaggcc gaccaccgcg gcgatgtcgc ccgcgcgcac ttccttgatt
      841 tcctggcgct cgttggcgtg catctgcagc aggcgcccga tgcgctcctt gcggccgcgc
      901 accgaggtat gcaccgtgtc gcccgaggcg agcacgcccg agtacacgcg gatgaacgac
      961 agctggccga cgaacgggtc ggtcatgatc ttgaaggcga gggcggcgaa cggttcctcg
     1021 tcctccggct tgcgcacatc cggctcgccg ttctccttgg agcccttcac cggcgggatg
     1081 tcgacgggcg acggcaggta gtcgatcacc gcgtcgagca tcgcctgcac gcccttgttc
     1141 ttgaacgccg agccgcacag catcggcacg atctcgttgt tgatggtgcg caggcgcaag
     1201 ccgcgtttca tctcctcgat cgacagttcg ccggactcga ggtacttgtt catcagctct
     1261 tcgttggcct cggcggcggc ctcgaccatc ttctcgcgcc actccttcgc ctccgccgcg
     1321 agctcggcgg gcaccgcccg gcgctcgaac ttcatgccct gcgaggcttc gtcccagtaa
     1381 atcgcctgca tggtgaccag gtccaccacg ccctcgaact tttcctcggc gccgatcgga
     1441 atctggatcg gcacggggtt gcccttgagg cggttcttga tgtgctcgta ggacttgaag
     1501 aaggtggcgc cgacgcggtc catcttgttg atgaacgcca ggcgcggcac cttgtacttg
     1561 ttggcctggc gccagacggt ctcggattgc ggctgcacgc cggctaccgc gtcgtacacc
     1621 atgcacgccc cgtcgagcac gcgcatcgag cgctccacct cgatggtgaa gtcgacgtgc
     1681 cccggcgtgt cgatgatgtt gatgcggtgc tccgggtagg acaggtccat gcccttccag
     1741 aagcaggtgg tggcggcgga ggtgatggtg atgccgcgct cctgctcctg ctccatccag
     1801 tccatggtcg cggcgccgtc gtgcacctcg ccgatcttgt ggttcacccc ggtgtagaac
     1861 aggatgcgct cggtggtcgt cgtcttgccg gcgtcgatgt gcgccgagat gccgatgttc
     1921 cggtagcgtt cgatgggggt cttgcgggcc acgatgttca ccaggcgtcc gttagaaccg
     1981 gtagtgggcg aacgccttgt tggcgtcggc catgcggtgc acttcctccc gcttcttcat
     2041 cgcgccgccg cggccttccg cggcctcctg caactccccc gccaggcgca cggtcatcga
     2101 cttctcgccg cgcttctgcg ccgcctcgcg gagccagcgc atggcgagcg ccacgcggcg
     2161 caccgggcgc acttccaccg gcacctggta gttggcgccg ccgacgcgcc ggctcttcac
     2221 ctcgaccacc ggcttcacgt tctgcaccgc ctgcgtgaac acctcgatcg ggtccttgcc
     2281 cgacttggtc ttgatgacgt cgaacgcgcg gtacacgatg ccctcggcga ccgatttctt
     2341 gccacgggtc atcagcacgt tgatgaattt cgcgacttcc ggatgattga acttgggatc
     2401 cggcaggatc tcgcgcttgg ggacttctct gcgacgcggc atgggggcag gtctctcgtt
     2461 tacgccgact tgggcgcctt ggcgccgtac ttggagcggc tttgcttgcg gtccttcacg
     2521 ccctgggtgt cgagcgagcc gcgcacgatg tggtagcgca cccccggcat gtccttcacg
     2581 cgcccgccgc ggatcagcac caccgagtgc tcctgcaggt tgtggccttc gccgccgatg
     2641 tagccgatca cctcgaagcc gttggtgagc cgcactttcg ccaccttgcg cagcgcggaa
     2701 ttgggcttct tgggggtcgt ggtgtagacg cgcgtgcaga ccccgcgttt ttgcggcgac
     2761 tggcgcagcg ccgggacctt cgacttggcc ctcttggtcg aacgcccgaa acgcactaac
     2821 tgattgaccg taggcattat tttccgtatc cagaaatgaa ccgggcggcc acgtccagcg
     2881 aaccgtagcc gcccaggcgc aaaaaagagg ccggattata aggaggaagc cgggccagcg
     2941 caaggaaaac gcggctcgga aacgaagccg aatcgcgcct cggcctcgcc gcggcgcagc
     3001 ctcgcgcccg gcgcggcggc atcggcatag cgcgcgaggt cccggtcatc gagcagcccg
     3061 agtccgcggc tggtcagcat caggaccgat ccctcgtcgt ccaggtaggt ctcgagcgcc
     3121 gccgccggcc gcgcgcagtg gtcgaacagg gagtttccct cgtagtgcag gaccagcggc
     3181 gcataggcaa gccgcacgta cacccgctgc ggcccgttct ggaagtacca gccgccgcgc
     3241 tcgtccggcg cgtaattgcg cgagatgaat tcgcgcaatg ccgcgttgcc gatgcgccca
     3301 aaacgcccgt cggccgtttc ccgcagcagc cagttgccgc gccggtcgag cgccagccag
     3361 ccgtagaccg ccggcacatc cggccatttc gccaggctgc ccaggacctg cgggtccacg
     3421 cctactgccc ggcgggccgc tgcgcgatgg gcgcgggccg cgcgatgccg aagccctgcg
     3481 cgaaaccgac attgagcagc ttgagccgcg cgaggatgtc ggcatcctcc acgcattcgg
     3541 cgatcacgcc gacgccgatc gcctcgccca cgcgcacgat ggccttgagc ttctgctcgg
     3601 cgacgccgct gcgcagcagg ttgcgcacga tcgagccgtc gaccttgacg taatccacgc
     3661 gcagcgactt cagcggcgcg aacgacaccg agcgctggcc gaagccgtcg atcgccacct
     3721 tgcagccggc ggctttcatc gcggcggcga accccgccgc cgacgccgtg cgcgccagca
     3781 ccgaggcttc ctcgatctcg aaggtcaggg ccgcaggcgc aatcctggtg ctgcgcagtt
     3841 ccgccgccac gaaggcggga aaatcctggt cgccgatggt ctgcaccgac acgttgacgc
     3901 tgaaactgcg gaagccgccc ggccgccctc cggccagcca gcgcagcacg tggctcacca
     3961 cccagcggtc cagctccggc atcatcccgt agtgctcgaa caccggcagg aaatcccccg
     4021 gcggcagcat cagggtttcc tcctcgcgca ggcgcaccag cacctcggcc atcgcgaatc
     4081 cgccgggcgg gtgcagcgac aggatgggct ggcaatacag ctgcagttcg tccaggtcga
     4141 gcgcctggcg caggcgcgcg gacgggtcgc gccacccggt gagctcctgg ctcatccggt
     4201 cgaggaactg gtcgtcgccg ggcgcggggc ccgtttttga tgtcgtcgtc gccataggcg
     4261 gtccgtcgcc ctattctagc cgcgcgcgcc gcgccgccaa gctaacattg gcgcggttta
     4321 tcctggagac cgacccgatg gccagagagg acttcgcctg cacgcaccgg ctgcgcgtgc
     4381 gctgggccga agtggacatg cagaagatcg tgttcaacgg ccactacctc acctacatcg
     4441 acaccgcggt cgccgactat tggcgcgcca tcggcctgcc ctacccggag ggctatgtgg
     4501 agcgtttcgg cagcgacatg ttcctgcgca aggccacggt cgaatacctg ggctcggcgc
     4561 actacgacga cgcgctggag gtgctgtgcc gcacggcgaa gctcggccgc accagcatga
     4621 ccttcctgtt cgagatctgg cgcggcggcg acctgctgat cggcgccgaa ctggtctatg
     4681 tgaacgccga tccccgcagc aggaaggccg tgccgctgcc tgaagagctg cgccgctgcg
     4741 tgctcggctt cgagaaagtc gcgcccgtcc aatgactctg tactgggagg atttcaaggt
     4801 cggcgactgc gtggaaatgg gcagccacag gttcagcgag gaggagatca tcgccttcgc
     4861 ccggcagttc gatccgcagc ccttccacat cgaccccgtg gcggcgaagt cgtcggcgtt
     4921 cggcggcctc atcgccagcg gctggcacac ctgcgcggtg tgcatgcgcc tcaattgcga
     4981 ttcctacatc aaccgcacgg tcagcctggg ctcgcccggc ctggacaaca tccgctggct
     5041 gaagccggtg cggcccggcg acaccatccg ctttcgccgc accgtgctcg acgcgcgcgc
     5101 ctcgctgagc cggccgggcg tcggtctgct gcgctcgcgc acagaggcac tgaaccagca
     5161 cggcgtcgtg gtgatgtcga tggagggctg gggcatgttc ggccgccgcc ccgcggcgtg
     5221 agcgccttca ccgtcgccgc actggcggtc taccccgtga agggcgccgc cgcggtggcg
     5281 ctgcaggcat tcgaggtgca ggactgcggt cccgacgcgg accgccgcta ctgcctggtg
     5341 cgtcccggcg gcagggtgtt cacgcagcgc gacgaccccg gcctcgccag gttgcgcgcc
     5401 gcgctgcgcg gagagcacct gtcgctcgag ttcgacggcg agcaactcgc ggtcggtccc
     5461 ggggcgttcg tcgccaccgc cgagatccgc gtctggagcc ggagcgcgac ggcgcgcgtg
     5521 gccgacgccg gcaccaatgc ctgcgtcagc caatggttcg gcaccccgct gctgctggcg
     5581 aagctcgagc acccgctgca gcgcgagggc gccgcgctcg ctttcggcga cgccgcagcg
     5641 ctgctggtgg cgaacctggc gtcgctcgac gcgctcaacg ccgccttgct ggcgccagtt
     5701 cccatggacc gcttccgccc gaacatcatc gtgggaggcg cggcggccta cgcggaagac
     5761 ggctggcgca aactgcgcgt cggcggtgcg ctgctggccc cggtgcatcc ctgcggccgc
     5821 tgccaggtga ccaccatcga ccaggagttc ggcgcggtgc tgggcgacga gccgctgcgc
     5881 acgctcgcac gcctgcgcac cagggacggc gaggcggtgt tcggcgtgcg ctatgcggtg
     5941 gagcgccccg ggcgcgtgcg cgtcggcgac cgcgtcgagc cgctcgatca gtgatagcgc
     6001 tcgtagccgg cgaagctgat cagcaggcgc gcgatgccgt atccgcacca ggtcaggacg
     6061 cgctcccaca gcggctggcg cttccactgc agcgctgcga ggcggctcgc gccgacgcgc
     6121 atggcttcct ccaggctggc gcgcagcccg gcggcgaagc gcgcgtcgtc gacgaacacg
     6181 ttcgcctcgc gcgccagcag caggctgaac ggatcgatat tggtggaacc cacgctcgcg
     6241 cggcagcagt cgaacaccgc gaccttggcg tgcaggaagc cgcgccgata ctcgtggatc
     6301 tcgatgcccg cgtcgagcag cgcgccgtac agcgcccgcg aggcgtagtg ctgcaggagg
     6361 tactcgacgc gtccctgcag caacagcacc acgcgcacac cgcggtgcgc agcgcgcagc
     6421 agcgcctggc gaaaacgccg gccgggaaag aagtaggcgt tggcgatcac cacctcctcg
     6481 cgcgccgcgt cgatcgaagc gagataggcg cgctcgatgt cgcgccggtg gcgcacgctg
     6541 tcgcgcacca ccagcgccgc ccgagttccg tcggggaaag acccgagcgg cgcgggcgcc
     6601 tgcgcctgcg tcgcgcgccc cgagcggccc gccacgcgct cccacagggc tgccgcctcg
     6661 gcgcgtacgg tcgcggtcag cggcccctcg atgcgcaccg cgtagtcggc gcgcggcggc
     6721 gccgggtcgg gtccgtcgta gtcgtcgacg atattgatgc cccccacgaa agccacccgg
     6781 ccgtccacgc atgccagctt gcggtgcatg cggcgcagcc ggtcgcgccg cagcgtccac
     6841 gggctgatgt tcgggcggta gaccaggaag cgcgccccgg cgcgggcgag catccgccgg
     6901 aagcgcggcg cgaagccgcg cgagccgaaa ccgtcgacca gcaggtgcac cgacgcgccg
     6961 cgcgccgccg cgcgcgccag cgcgtcggtc accaggctgc cggtctggtc gtcggcgtag
     7021 atgtagcttt cgacgaacac ctcgcgccgc gcgccctcga tcgcctcgac cagcgccggg
     7081 aaatactgct cgccgttgcg cagcaggagc aggcgattgc cctcgacgta atgcatggcg
     7141 ctagtgcggc ttgagccgcg cgcccagcgc gaggtggtcc gacagcagcg accagggcgg
     7201 gccgcggtgc acgcgcgcct cgaccacgct gaagccccgc acatagatgc ggtccagccg
     7261 cagcagcggc agcaggctcg gaaaggtgcg cgccgcgtgg cggccgtacg ccgacgtcac
     7321 ctcggtcatg ccgaggcgct gcgccagcac ccgcgtcgcg cgctggcgcc agtcgttgaa
     7381 atcgcccgcc aggacgatcg gcaggtcgcg ccccgcaagc gcgtcgagcc gtgcgctgac
     7441 cgcctcgagc tgccggcggc ggccgccctc gtgcagcgac aggtgcacgc acacgcagtg
     7501 caggttgcgt cgccagcccg gcaccgccac cacgcagtgc agcacgccgc gccgctcgaa
     7561 gcggtggtcc gacacatcct cgttctcggt ggacaggatg cgaaaacgcg acaggatggc
     7621 gttgccgtag tggccgtggt ggtggacccg gttgcggccg taggcgtgct gccagtccgc
     7681 gggcgccagg aactcgtgct gcggcgcgtt cggccaggag ggaaagcgca cggcgtggcg
     7741 ctcgttgaga ccctggactt cctgcaggaa caccaggtcc ggcgccagcg tgcgcaggcc
     7801 ctcgcgcagc tcgtgaatta ccacgcggcg gttgaactgc gacagcccct tgtggatgtt
     7861 cagcgtcacc acgctgagcg actcggtcac gccgcggcgc tcctcgcggg cagcatgcgg
     7921 atggcgtcgc ggttcgacca ggaaaagcat ttctccgccg cttcccgcca cggcaaccac
     7981 tgctgcgcgc gatgctcctg cgccgcgagc gtcaccgcga tgcgctgcgg cagcatcagg
     8041 ccgaacacgt gctcggtgtt gtgcgtggtg ccgggcgcga agcgccggcg ccagtgcgcg
     8101 tagatctcga atgcgttggc cagatgccag ggcaccagcg ccgggccgtc ggcgtcgatg
     8161 ccggtctcct cgcgcagctc gcggcgcgcc gcgtcctgca gcggctcgtc gagccggtcc
     8221 agcgagccgg tgacggactg ccagtagccg gggcggctgg cgcgctcgag cagcaggacg
     8281 tcgagggccg cagtgtggat caccaccagc accgagacgg gcagcttcgc gcgcatcagg
     8341 ccttcggccg gaacaatgcc ggcagcgcca tcgcgttccc ggggtcgatg aacaccgcga
     8401 agaacggctt gccgcgcccg gcccagtgct cggcggcggc gcgcagcacg tcgaccagca
     8461 cccccagatc gtcggacggc agctcgccgt gcccgccgat cagcaccaca tggccgtcgc
     8521 cggcgcgcca ggaaaggtcc gccaggcagt cttccagggc atcccagttg ccgccgaacc
     8581 acgcggggaa ggcgagcgcc gccgccagcc tctcgagcag cgattccctg tcggtcgcgc
     8641 cgcccaggtc gacgccggcg aggtcgaggc gcgtgccgcg cgcgccgtcg aggacctcgt
     8701 cggcccgcgg cgcgcggtac acgcccgagc gcgccgcgtc ggtcaatcgc tgcgtgagtt
     8761 tgcccatggg ttactcgcgg atgcgctgga acgtacgata gtggtctgcc gtgtagtaga
     8821 actcgccgtc cttgccggcg acgatgcgcc gggcgccgcg atcgcgcgcg ccgggcgtcg
     8881 tcaccgtgta ctcgcgatag tagccgcgcg cgcgccgcgg cagcagcttt tcccggttgc
     8941 cgaaggccct gccgtcctgc tggtggggga aaggaccgcc ggccctgatc agcgccagcg
     9001 tggcgcgcgc ctcgggcggc agcccggcga gcggaatgtc gcccgcccag gcattcgcgg
     9061 cgaacgccag aaccagggcg gcgagccgga tcactgcggc gcgttgcgca gcctgatgtg
     9121 cagttcgcgc agctgcttct cgctcacggc cgagggcgcg ccggtgagca ggtcctggcc
     9181 gcgctgcgtc ttcgggaacg cgatcacgtc gcggatcgcg tcggcgccca ccatcagcgc
     9241 caccagccgg tcgaagccga aggcgatgcc gccgtgcggc ggcgcgccgt actgcagcgc
     9301 gtcgagcagg aagccgaact tggcgcgctg gtcctccgcc gagatcttca ggccgcggaa
     9361 cacccgctgc tgcagttcct ggcgatggat ccgcaccgag ccgcccgcca cctcccagcc
     9421 gttgaggacc atgtcgtagg ccttggcgta ggccttctcc ggcgccgact ccaggtactg
     9481 ctcgtggccg tccttcgggc tggtgaaggg gtggtggcgc gccttccact gccgcgactc
     9541 ctcgtcgtac tcgaacatcg ggaaatccac cacccacagc ggccgccagc cctgcgcggc
     9601 caggccgcgc tcatggccga tcttctggcg cagggcgccg aggcaggcat tggcggtctc
     9661 cgccttgtcg gcggcgaaga aaaccaggtc gccgtcggcg gcgccggtgc gctccaggat
     9721 cgccgcgatc gccctggcgt gcaggttctt cacgatgggc gactgcagcc cgtcggcgcc
     9781 cttcgcgcgc gcgttcacct tgatccaggc gaggcccctg gcgcccagcg ccttcacgtg
     9841 ctcggtgtag gcgtcgatct cgccgcgcgt cagcgcgccg ccgccgggca cgcgcagccc
     9901 cgccacccgg ccgccgggca tgcccgccgg cgccgagaac accttgaagt ccacgtcgcg
     9961 caccacgtcg gtgagttccg cgagcttaag cggcacgcgc aggtccggct tgtccacgcc
    10021 gtagtcgcgc atcgcctcgt cgtagctcag caccgggaac gggtcgggaa gcgcgacgcc
    10081 cagcacttcg ccgaacatgt gccgcgccag ccgctccatc agcgcgcgga tctcctgctc
    10141 gccgagaaac gaggtctcga tgtcgatctg cgtgaactcc ggctggcggt cggcgcgcag
    10201 gtcctcgtcg cggaagcact tgacgatctg gtagtagcgg tcgaatcccg cgaccatcag
    10261 cagctgcttg aacagctgcg ggctctgcgg cagcgcgtag aactgcccgt cgtgcagccg
    10321 cgacggcacg aggaattcgc gcgcgccctc cggcgtcgac ttgtacagca ccggcgtctc
    10381 gatgtcgacg aagccgttgg cgtccaggaa gccgcgcgcc gcgcgcgccg ccgcgtgccg
    10441 caggcgcagg ttgtgctgca tctgctcgcg gcgcaggtcg accacgcggt gctccaggcg
    10501 cacctgctcg gacaggttgc cctcgtcgag ctggaacggc ggcggcacgg ccgggttgag
    10561 gatctccacc tcctgcgcca gcacttcgat ctcgcccgac ttcaggttcg ggttggtggt
    10621 gcccgccgga cgcgcgcgca ccagcccggt gacgcgcagc acgaattcgc cgcgcacgcg
    10681 gtcggcggcg ctgaacgcct cggcgcggtc cgggtcgcag accacctggg cgaggcccgc
    10741 gcggtcgcgc aggtcgatga agatcacccc gccatgatcg cggcgccggt gcgcccagcc
    10801 cgccagggtc accttctggc cgatcagcgc ggcggacagg tcggcgcagt aatgggtgcg
    10861 catggcccgc ttacctgccg ccctggccgg gggcgaccac gcccatcgag atgatgtact
    10921 tcagcgccgc gtccacgctc atgtcgagct cgatcacgtc ggcgcgcgcc agcatcacga
    10981 agaagccgct ggtcgggttg ggcgtcgtcg ggacatagac gctgacaaag tcgccctgca
    11041 ggtgcgtcgc cacgtcgccg cccggcgtgc cggtgaggaa ggcgatggtc cacatgccct
    11101 cgcgcggcca ctgcaccagc agcgccttgc ggaacgcctg gccgctggag gagaacagcg
    11161 tgtcggagat ctgcttgacg ctcgaataga tcgaattgac caccggaatg cggtgcagga
    11221 ggtcgtgtcc cagctgcacc aggcgggcgc cgaagaaatt ggtcgcgaac acgccggtgg
    11281 ccaccacgat caccagcgtg aggatcaccc ccaggccggg aacgtgcacg ccgaggaagc
    11341 cctccgtgcg ccactgctca ggcagcagca gcagcgactg gtcgagggta tcgacgatca
    11401 gcttcaggac ccacagggtg atgaccagcg ggatccagat cagcaggccg gtgatgagat
    11461 atttcttcat gcttccccgg aaaacaaaag cccggctcgc gccgggccgc tcgcgaggcc
    11521 cgtcagggca tcagttggta gtggtggcag gcgccttgtt cgccttggca ggcttcgccg
    11581 gctccgcctt cgcttccgtc ttgctgtcgg ccttcggctc agccttggct tcgctcttcg
    11641 cttcgcccgc ggcatcggcc ttctccgccg cgggcttggc gccccctttg aagtcggtcg
    11701 cataccagcc gctgcccttg agctggaacc cgggcgcgct cagcattttc tcgaacttcg
    11761 ccttgccgca ggacggacag accttcagcg ggcgttcgga caccttctgc aggaattcat
    11821 cctggaaacc gcaggcggcg caccggtact cgtaaatcgg catggctgtg ttccaagctc
    11881 ctgaatctgc gccgaattat agcgccatcg ggcaggctag aacggaatgt cgtccgccat
    11941 gtcgtcgaac ttgccggcgg gcttcttcgc gccgctcttg ggctcggagg ccggctcgct
    12001 ggcgccgccg ccggcgggcg caccgcgcgg ctcgcccgcg ccggcgcgcg agccgagcat
    12061 ctgcatcacg tcggcgcgga tctcggtgct gtagcggtcg tgaccttcct tgtcctgcca
    12121 cttgcgcgtg cgcaggctgc cttcgacgta gacctgcgag cccttcttga ggtactcgcc
    12181 cgccacttcc gcgaggcggc cgaagaatgc gatgcggtgc cattcggtcg cttccttcat
    12241 ctcgccgctc gccttgtcct tccagcggtc ggtggtggcg acgcggatgt tggtcaccgc
    12301 ctcgccgctc ggcagatagc gcgtctccgg gtcggccccg agattgccca ccacgatgac
    12361 cttgttaacc gatgccatgc ctacctccct ttcaccgttc ccatcgtaaa cgactcacca
    12421 ccgatccggt tgactggggc aagggacgtc tggaaacgaa gacctggaac tcgttatagt
    12481 agcaagttta cttttgcctt ccggcgcgat ggacagcagc ccgaccagca cgatacgcat
    12541 ccgcggcgcg cgcacgcaca acctgaagaa catcagcgtc gacctgccgc gccaccgcct
    12601 ggtggtgatc accgggctgt cgggctcggg caagtcctcg ctcgccttcg acacgctgta
    12661 cgccgaaggc cagcggcgct acgtcgagtc gctgtcggcc tacgcgcgcc agttcctgca
    12721 gatgacggag aagcccgacg tcgacctgat cgaggggctg tcgccgtcga tcgccatcga
    12781 gcagaaggcg ggcagccaca acccgcgctc caccgtcggc accatcaccg agatccacga
    12841 ctacctgcgc ctgctgtacg cgcgcgtcgg cacgccctac tgccccgacc acgacctgcc
    12901 cctgcaggcg caatcggtgt cgcagatggt ggaccaggtg ctctcgctgc cgccggacac
    12961 ccggctgatg gtgctggcgc cggtggtggc cggccgcaag ggcgagcagg cggacctgct
    13021 ggaggaattg cgcgcccagg gcttcacgcg cgtgcgcgtg gacggcaagg tgctcgagct
    13081 cgaggcggcc ccgcgcctgg cgaagaatct caagcactcg gtggacgtgg tggtggaccg
    13141 gctgaaggtg cgcgccgaat cgaagcagcg cctggcggag tccttcgaga ccgcgctgcg
    13201 ccacgccgac gggcgcgcca tcgccgtcga ggcggattcc ggcaaggaac accagttctc
    13261 cgccaggttc tcctgcccgg cgtgcgccta caccctgccc gagctggagc cgcgcctgtt
    13321 ctcgttcaac aacccgatgg gcgcctgccc gcgctgcgac gggctgggca gcgtcgagtt
    13381 cttcgacccg cggcgcgtgg tcgcgcaccc caacctgagc ctcgccggcg gcgcgatccg
    13441 cggctgggac cggcgcaacc atttctattt caccatgctg caggcgctcg cgcggcgcta
    13501 cgccttcgac gtcgaggcac cctgggaggc gctcgccgac gaggcgcagc aactgatcct
    13561 gtacggctcg ggcaaggaaa agatcgcctt ctcctacccc ggcgagcgcg gccgcaccgc
    13621 ggtccgcgag cacgccttcg aaggggtggt gcccaacctg gagcgccgct accgcgacac
    13681 cgactcggcg gtggtgaagg aggagctggc caagtaccgc aacaggcgca cctgcccgga
    13741 gtgcggcggc acgcgcctgc ggcgcgaggc gcgccatgtg cgcgtcgccg acaggaacct
    13801 gcacgcggtg tcctgcctgc cgctgcgcca ggcgctcgag ctgttccgcg gccttggcgc
    13861 gaccctgcag ggcgcgcgcg ccgcggtcgg cgagcggatc gtgaaggaga tcgccgaccg
    13921 cctggagttc ctggtcaacg tcggcctcga ttacctgtcg ctcgagcgct cggcggagac
    13981 gctctccggc ggcgaggcgc agcgcatccg cctggcgagc cagatcggct ccggcctgac
    14041 cggggtgatg tacgtgctgg acgagccctc catcggcctg caccagcgcg acaacgcgcg
    14101 cctcatcgag accctcaagc gcctgcgcga cctcggcaat tcggtgatcg tggtcgagca
    14161 cgacgaggag gcgatccgcg ccgccgacta cgtgctcgat atgggccccg gcgcgggcga
    14221 agccggcggc cacgtggtgg cgcaagggcg ccccgaggac gtgatcgccg atccgcagtc
    14281 gctcaccggc cgctatctcg ccggcacgct gcagatcgac gtgccgcgcc tgcgcaaccg
    14341 gcccggcaag aaccgcctgg tggtgcgcgg cgcgagcggc cacaacctga aggaggtcga
    14401 cctcgagctg ccgctcggcc tgctggtgtg cgtcaccggt gtctcg
//
LOCUS       DASRIE010000250        17771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2328399, whole genome shotgun sequence.
ACCESSION   DASRIE010000250 DASRIE010000000
VERSION     DASRIE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328399"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1225
                     /gene="ftsZ"
                     /locus_tag="VFB53_10870"
     CDS             59..1225
                     /gene="ftsZ"
                     /locus_tag="VFB53_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZN87272.1"
                     /translation="MFEIVDSQAAGPVIKVIGVGGAGGNAVNHMIEQGVEGVEFIAVN
                     TDAQVLGRNKSRNQIQLGSSGLGAGAKPEEARAVAIADRERIEEAVAGAHMVFITAGM
                     GGGTGTGAGPVIAEVSRALGILTVAVVTKPFSFEGSKRMKIAEQGLAELSPHVDSLIV
                     ILNDKLEEVLGEDVTQEEAFKAADDVLNSAVAGIAEIINQPGLVNVDFQDVRTVMSEQ
                     GMAMMGSAAASGIDRARIAAEQAIACPLLEGVNLAGARGVLVNISASRASLKLRETKE
                     VMNTVRAFAADDATIIYGGVYDDALGDELRVTVVATGLGAPIAARQPKPQLVVSQKTG
                     TDNQPLMAAGAVDYSQLESVPAVIRRNRASTVEALQQSGVDKYDIPAFLRKQAD"
     gene            1328..2242
                     /gene="lpxC"
                     /locus_tag="VFB53_10875"
     CDS             1328..2242
                     /gene="lpxC"
                     /locus_tag="VFB53_10875"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZN87273.1"
                     /translation="MLKQRSLKSLIRAKGVGLHTGEKVTIVLRPAQPDTGIVFRRVDL
                     DPPVDVPARADLVGETRLSSCLVHQGAKVYTVEHLMSALAGLGVDNAYVDIDGPEVPI
                     MDGSAAPFALLLQQAGLEEQPAPKRFLRVKRAVEVRDGDKWARLEPYEGLRLAFSIVF
                     NHPVIDKTSQSVTVDFAETSYLKEIARARTFGFMHDVERLREDGLALGGGLDNALVLD
                     EYRLLNADGLRFADEFIRHKVLDAIGDLYLLGRPLLGAFSAHKSGHALNNRLLRALLA
                     DAVNWEMATFERADQAPAGVAGLVLQPV"
     gene            complement(2249..2755)
                     /locus_tag="VFB53_10880"
     CDS             complement(2249..2755)
                     /locus_tag="VFB53_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZN87274.1"
                     /translation="MQPARIDRYIADDEGLRPIVAKAQDIRTLTRLCADFLPAGLARQ
                     LRVAHLSDGELSLLVTNAAAAAKLKMLAETLRKFLLRQGSKVSLISVRVQPSNAQSEL
                     RPARKEATLSSAGISELSALYGRLAPGSPARLALGLLLRRQRLRPPPAAVPRKTGAAR
                     GRAGKARS"
     gene            2835..3734
                     /locus_tag="VFB53_10885"
     CDS             2835..3734
                     /locus_tag="VFB53_10885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN87275.1"
                     /translation="MSARHLLASLAAAGILLIALTAALYWVTFRFATDIKIPLVRELV
                     LSAQQGEAERARKFVQQNLNAMAVKLGEMQAQLARLDALGDRVSAMAGIRPQEFRFTE
                     APGLGGAQPTALPPQNLSLAEFGEKLYALSRSVENRTDMLGVLETRLFEETIKKKMMP
                     TSLPVSAPYNASSFGMRIDPFTGMQAMHEGIDFLADLGSPVTAAAGGVVTFAGFHPQY
                     GNVVDIDHGNDLVTRYAHLAKLLVKEGDIVQRLHRLGLSGSTGRSTGPHLHFEVRFRG
                     VAQNPTRFLFALQGTQQAARSGR"
     gene            3823..6576
                     /gene="secA"
                     /locus_tag="VFB53_10890"
     CDS             3823..6576
                     /gene="secA"
                     /locus_tag="VFB53_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZN87276.1"
                     /translation="MITRVLTKIFGSRNDRLLRQYARTVVRINQLEAGVAALSDDALR
                     AKTGEFKRRLAERVAAAPAEERDAAEGEALEALLPEAFAVVREAGKRTLRMRHFDVQL
                     VGGMVLHDGKIAEMRTGEGKTLAATLPAYLNALAGRGVHIVTVNDYLARRDADWMGKI
                     YGFLGLSVGVNVPQMEPEDKRAAYAADITYGTNNEFGFDYLRDNMAMRAEERFQRSLR
                     YAIVDEVDSILIDEARTPLIISGQAEDRTEVYARINEVAPLLTRQKDEKGEGDFWVDE
                     KNHQITLSEAGHARAEEILARAGFLPKGASLYEPAYINLVHHLYAALRAHNLFHRDQH
                     YVVQDGEVIIVDEFTGRLMQGRRWSDGLHQAVEAKEKAAIQNENQTLASITFQNYFRM
                     YRKLAGMTGTADTEAYEFQEIYGLETVIVPTHKPMIRVDRLDLVYRTAKEKHKAIIDD
                     IKDCHGRGQPVLVGTTSIEASELLSGLLTKEKLPHQVLNAKQHAREAEIVAQAGRPKM
                     VTIATNMAGRGTDIVLGGNVEKQVELLEATGDVAAADKQTRIAKLGSEWQALHDHVIR
                     EGGLHIIGTERHESRRVDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFAGERINKIMV
                     MLRMPEGEAIEHAMVTRSIESAQRKVEARNFDIRKQLLEYDDVANDQRKTIYALRNEL
                     LDAQGVAERITDLRAGVIADMFRSCVPPESVEEQWDIKGLEAALKSELNLALPVGQWL
                     EKEPELGDEALLERITQKAHESYRGKIPSGAEDSFHQYERYMMLQVLDAHWREHLAAL
                     DHLRQGIHLRGYAQKNPKQEYKREAFELFGAMVEAVKLEVTRTLVAVEIRASDDVPQA
                     DVGQHVENVRLQHADYDTALAAGAEERKPQPAVRPAQKVGRNDPCPCGSGRKYKHCHG
                     KLS"
     gene            6581..7792
                     /gene="argJ"
                     /locus_tag="VFB53_10895"
     CDS             6581..7792
                     /gene="argJ"
                     /locus_tag="VFB53_10895"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZN87277.1"
                     /translation="MAVNLRAPDAAQLLPVAGIELGIAMAGVRKAERKDLLVVRVAPG
                     TAVAGVFTRNRFCAAPVVLAKKHLRNPVHALVVNTGNANAGTGDDGLLRALAVCRALA
                     GALRCRPEQVLPFSTGVIMEPLPVERIVAGLPRAVADLRAANWASAAEAIMTTDTVPK
                     AASRQVRLSTGLATVTGIAKGSGMIRPDMATMLGFVATDAKLPRGSLQRLTRSAADQS
                     FNAITVDGDTSTNDAFICLATGKGPVARSGKDEAVLARAIGEVAQWLAQAIVRDGEGA
                     TKFVTVRVEGGRSAAECRKVAYAIAHSPLVKTAFFASDPNLGRILAAIGYAGVAGLDT
                     SKVDLYLDEVLVAKGGGRHAGYREELGAAAMKKPEFTVRALLNRGKAAATVWTCDLSF
                     DYVKINAEYRS"
     gene            7789..8649
                     /locus_tag="VFB53_10900"
     CDS             7789..8649
                     /locus_tag="VFB53_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN87278.1"
                     /translation="MSQDIDKLLQKLEQLLPPAPPPTDWSASIAFRWRKSAGLNARGW
                     LQPVRHVHRMRLSDLQGIGPQIAKVEQNTRQFIEGRPANNVLLTGARGTGKSSIVKGL
                     LNKYHKQGLRLIEVEKNDLVDLAAITDLVAGREERFLVFCDDLSFHGAEDGYIALKVA
                     LDGSISTTSENVLIYATSNRRHLMPEYMQENLETRYVGDEIHPGETVEEKISLSERFG
                     LWVSFYPFDQDEYLEIVAHWLTTLGCGAREAEAAREESLQWSLQRGSRSGRVAYQFAR
                     DWAGKHAKKK"
     gene            8651..9577
                     /locus_tag="VFB53_10905"
     CDS             8651..9577
                     /locus_tag="VFB53_10905"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HZN87279.1"
                     /translation="MAIEVAAAVIQRADGAFLLARRPAGKAYAGYWEFPGGKVEAGEA
                     AERALVRELHEEIGIDACTAYPWITRVFAYPHATVRLNFFRVTAWKGDPHPKEEQAIA
                     WQHVGAPLAEPMLPANAPVLAALALPHEYAITDAGSSGAGAMLSRIRPRLAQGLKLVQ
                     VRDRGLPQREEFIATVVRLAHRHGARAMVSGGPALGDGIHFTAAELMRLEERPAGMLA
                     AASCHTAEELRRAMDLELDFAVLGPVKSTPSHPGARPLGWDGFARIARGASIPVYAIG
                     GMQRSDLEAAWRAGAHGVAMISGAWQGLRRDA"
     gene            9885..10484
                     /locus_tag="VFB53_10910"
     CDS             9885..10484
                     /locus_tag="VFB53_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN87280.1"
                     /translation="MRLRIFSNAGKHGERTMGAIEMSMDLDFTKGVRAANPTLPDAAA
                     LPPSEIGASRPELGGNCIFYVRAGVARPASADAPILVVEDDPDTRRLLEKVLALQGFA
                     VRGAADSREFQEAIRRPPLPQLILLDVELPRISGFKLLTALRQHPQTSAIPVVMVTAR
                     TANKDLLYGLSLGADGYLSKPFTVDGLRSMIDRVLPRRA"
     gene            10506..12005
                     /locus_tag="VFB53_10915"
     CDS             10506..12005
                     /locus_tag="VFB53_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN87281.1"
                     /translation="MNSQATILYARLHNVPPPDDATPGTCGAELLQEVRAVLQEPIAR
                     HEGIVAQQRPDSVLAIFGNDQREKPDHARRAISAAVQAVHDLARLNRRFAARMPQSPP
                     PRLALATGVHLGNVDVAPARNGSGAAVRATGEAVEVARLLECTAPDLGWSIVASQQAR
                     RAAGLPVESGRFGSVALPDSSFVDIAEITGLAPQRGPGNAAATYQALREAIVFNQRLQ
                     DRPRDLSGAAARAARNAAMHFSIEGYRILRKIGEGGMASIYLAAGPSEEPQVLKVMRI
                     MSGGQDDSLQRFIQEFALLAQVKHPNVAQIHRQGFSAGHAYIAMEHFSQGDLRARIAQ
                     GISRADAIAYVKQTAAALQAIHRAGIVHRDLKPDNLMLRRDGGLALADFGIAKHVAMS
                     ITDTAHGEVVGTPYYLSPEQAMGQQVDQRCDLYSLGIILYEMLTGTKPYLASTAQDLL
                     DLHVNGPVPTLRPPHQDLQPILERLMAKDCDRRYPSAQHLLAHLFALGL"
     gene            12002..14107
                     /locus_tag="VFB53_10920"
     CDS             12002..14107
                     /locus_tag="VFB53_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN87282.1"
                     /translation="MSPQATAPAIRVLAVDDDPSMRSLVSHLLGRAGYAVDVAADGGE
                     GLAKIRAQAPDLVVCDIQMPNMDGFAMLDAVRSDTATASLPFVLLTALTDRDSVRRGM
                     RLGADDFLSKPVRGHELVEAVSVALDGRRRLSGLVSGRALPEQRELRERYERKIDGGE
                     PQAARETSFAGMTGRMLTQTVLFSDIRGFTSMCERLPVPEIAELLSRYLREACRPILQ
                     ERGRIMKIMGDGLMAIFGQDAPHDVAAHAAAALRAGERIIDVAQEFRRWIASRFDLPG
                     LPPFDAGVGIHTGQVMLFQLSVGGAGDLTAVGDTVNVAARLEAKSKELGWPIVASMAT
                     IEHAGAHARVGEIREVVLAGRDARIQVGRINLLDAGAAEPRVQLPLTAGIEVVLDESA
                     RATAEAAKQALDRTLHAIGEQLHQYPATSEAEPVIHGYRVLAKIGEGGMSSVFLAEEQ
                     ARHRKVVLKVLKGKRVDDDGLWKRFFQECAILSSIKHDHVVRIYDQGFGDELAYIAME
                     HLAGGSLRELMDRGLSPRQALSLLSQAASGLAEIHRCGIVHRDIKPANLMLREEGVLV
                     LTDFGVAKRLETAAGQTMHGEILGTPYYISPEQSQGGAVTPRTDLYSLGVIFYEMLTG
                     ARPFVGDTLLEILAQHTAAPIPRLPAALAAYQRLVDGMLAKRPAERFDGAEAVLAEID
                     RVWTQHALLKASREAAAAH"
     gene            14212..15084
                     /locus_tag="VFB53_10925"
     CDS             14212..15084
                     /locus_tag="VFB53_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN87283.1"
                     /translation="MTQPDNHYSIDVFGFNQTERLMLSSIFGLAARRDPSFVQRDAGA
                     TRPADLYLVDGSDPAAMSEFHAVRARYPAPTVLVGDADPQSGLPAMPRPIQWARLLQS
                     FDDAVLGDAQPAPANSAAAGVFDKTMVLRRTGAPAPAAATPAAPMRPAAAARPQPRPK
                     QSTDTVLVVDDNATVRKFMEAKLAPYGFSADFAETGEQAVGLTGTKEYTCVFLDVVLP
                     GIDGYQVCKLIKSNKQAVKRTAVVMLTSRSSPFDKLRGSLAGCDEYLTKPVDENQLLE
                     VIAKFLPSARAAAA"
     gene            15113..15478
                     /locus_tag="VFB53_10930"
     CDS             15113..15478
                     /locus_tag="VFB53_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN87284.1"
                     /translation="MSKSILVVDDTRSMRKMVMTVLQAAGYSVEEAGDGAEALEKAQT
                     RVFDLVVTDHNMPRMDGVTLVGALRKLPAYDEVALIVLSTETGPELKAKGRAAGATGW
                     MAKPFDPEKMLGIVGQFIG"
     gene            15475..17550
                     /locus_tag="VFB53_10935"
     CDS             15475..17550
                     /locus_tag="VFB53_10935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZN87285.1"
                     /translation="MSVAAAKALGDASAFHALFFEEADEHLAAIEEILLRIDPAAAAG
                     EDLNAIFRAAHSIKGSSAMLGFPEIASLTHVLENLLDILRKGERALTREDVDALLRAG
                     DVVKMQLAFRRGAAAAAPDVDGTENELRALAVAAPATLRRYAVRLGPLAAPIEAAELD
                     TMLCGLSGMGEVHDRHLENIAGGEIRFGLALAGGEHDLRSVLTLVVAPELVHISAGDA
                     AASPDAPAATPPAATDDELFVDPAQWRRRANPEPTDVDLFVTPQAWRARRGAGRRAGD
                     DPVAPAEREGRREADRQAPAVQADATSIRVSVEKIDQLVNLVGELVITEAMLAQQHGG
                     LQKAGGHAELAGLGDLARHTRNLQEAVMAIRMVPISAVFSRFPRLVRELAQRLGKEVE
                     VKLSGEATELDRGLTEKLIDPLTHLVRNAIDHGLERPEVRLAAGKSRGGTVSLAASQR
                     GGRIVIEVRDDGRGLDRARILARAAERGASIAADAPDHEVWQLIFEAGFSTADSVTDV
                     SGRGVGMDVVRRNIQSLGGTVDLASSAGTGTTVTVSVPLTLAIIEAMTVAAGGQTYVV
                     PLASVVESRRVGRDEIRGIAGRGRTLRVRDDYLPVQRLAGQCARPQDESGDGGIAVIV
                     EADGAKAALLVDELIGQQQVVVKSLEANFRRVPGIAGATIMGDGKVALILDMTHLVNQ
                     AGRSQAPLH"
BASE COUNT         2931 a         6079 c         6302 g         2459 t
ORIGIN      
        1 cgcgagtggt tccagaggaa tttttgacgt cgtcttcact ggcaaggagg aaataccaat
       61 gttcgagatc gtagacagtc aggcagcggg gccggtcatc aaggtgatcg gggtgggtgg
      121 cgccggcggc aacgccgtga accacatgat cgagcagggc gtcgagggcg tggaattcat
      181 cgccgtcaac accgacgccc aggtgctggg acgcaacaaa tcgcgcaacc agatccagct
      241 cggctcgagc gggctgggcg cgggcgccaa gcccgaggaa gcgcgcgcgg tggcgatcgc
      301 cgaccgcgag cgcatcgagg aggcggtggc cggcgcgcac atggtgttca tcaccgccgg
      361 catgggcgga ggcaccggca ccggcgccgg gccggtgatc gcggaagtgt cgcgcgcgct
      421 cggcatcctc acggtggcgg tggtcaccaa accgttctcc ttcgagggtt ccaagcgcat
      481 gaagatcgcc gagcagggcc tcgccgaact ctcgccgcac gtcgactcgc tgatcgtgat
      541 cctcaacgac aagctggagg aagtgctggg cgaggacgtg acgcaggagg aggcgttcaa
      601 ggccgccgac gacgtgctca acagcgcggt ggccggcatc gccgagatca tcaaccagcc
      661 cgggctggtg aacgtcgact tccaggacgt gcgcacggtg atgtccgagc agggcatggc
      721 aatgatgggc tctgcggcgg cgagcggcat cgaccgcgcg cgcatcgcgg cggagcaggc
      781 catcgcctgc ccgctgctcg agggcgtcaa cctcgccggc gcgcgcggcg tgctggtgaa
      841 catcagcgcc tcgcgcgcca gcctgaagct gcgcgaaacc aaggaagtga tgaacaccgt
      901 gcgcgctttc gccgccgacg atgccaccat catctacggc ggggtgtacg acgacgcgct
      961 gggcgacgag ctgcgcgtga cggtcgtggc caccggcctg ggcgcgccga tcgcggcgcg
     1021 ccagcccaag ccgcagctgg tggtgtcgca gaaaaccggc accgacaacc agcccctgat
     1081 ggcggccggg gcagtggact acagccagct cgagtcggtg ccggcggtga tccggcgcaa
     1141 ccgcgcctcc accgtcgagg cgctgcagca gagcggagtg gacaagtacg acatcccggc
     1201 attcctgcgc aaacaggccg actgagccgg gccgcggtcg cggcggcgca gcgctttcgc
     1261 cctccttgcc gggggcgctg cgccgctcgc gtctgctaga atccgccctt cgctcaagaa
     1321 gttacgcatg ctcaagcaac gcagcctgaa gtccctgatc cgcgccaagg gcgtcggatt
     1381 gcataccggc gagaaagtga ccatcgtgct gcggcccgcg cagccggaca ccggcatcgt
     1441 gttccggcgc gtggatctcg acccgcccgt cgatgtgccg gcgcgcgccg acctggtcgg
     1501 tgaaacgcgg ctttcctcct gcctcgtgca ccagggcgcc aaggtctaca cggtggagca
     1561 cctgatgtcg gcgctcgccg ggctcggcgt ggacaacgcc tatgtcgaca tcgacggacc
     1621 cgaagtgccg atcatggacg gcagcgcggc gccgttcgcg ctgctgctgc agcaggccgg
     1681 gctggaagag cagccggcgc ccaagcgctt cctgcgcgtg aagcgcgccg tcgaagtgcg
     1741 cgacggcgac aagtgggcgc ggctcgagcc ctacgaaggg ctgcgcctgg cgttctccat
     1801 cgtcttcaac cacccggtga tcgacaagac cagccagtcg gtcacggtgg atttcgccga
     1861 gacctcctac ctgaaggaaa tcgcgcgggc gcgcaccttc ggcttcatgc acgacgtcga
     1921 gcgcctgcgc gaggacggcc tggcgctcgg cggcggcctg gacaacgccc tggtgctcga
     1981 cgagtaccgc ctgctgaacg ccgacgggct gcgcttcgcc gacgaattca tccggcacaa
     2041 ggtgctcgac gcgatcggcg acctgtacct gctcgggcgg ccgctgctcg gggcgttctc
     2101 ggcgcacaag tccgggcatg cactgaacaa ccggctgctg cgtgcgctgc tggcggatgc
     2161 cgtcaactgg gagatggcga ccttcgagcg cgccgaccag gccccggcgg gagtcgccgg
     2221 cctggtgttg cagcccgtct agcggacttc agctccgcgc ttttcccgcg cggccgcgcg
     2281 ctgcgcccgt tttgcggggt acggcggccg gcgggggccg gagcctctgg cgccgcagaa
     2341 gcagtcccag ggccaggcgc gccggagaac cgggcgcaag ccgtccataa agcgctgata
     2401 attcagagat tccggcactg gagagggtgg cctccttgcg cgcgggccgc agctccgact
     2461 gtgcattgct tggttgcacc ctcacagaaa ttaaactaac cttcgatccc tgccgtaaca
     2521 agaacttacg cagggtttcg gcaagcattt tgagcttggc cgcggcggcg gcattggtga
     2581 cgagcagcga gagttcgccg tcactgaggt gcgccacgcg cagctgtcgg gcgaggcccg
     2641 ccggcaggaa gtcggcgcac aggcgggtaa gagtgcggat gtcttgcgct ttggcgacga
     2701 tcgggcgcag gccctcgtcg tcggcgatgt agcggtcgat cctggctggt tgcaagagga
     2761 gtgggggaaa tagggagcgc cgggtgcaga ttatcgtaat ccccgatcgc ctggcgagag
     2821 ccaggacgct gaacgtgtcg gcccgccatc tgctggcgtc gctggcggcc gccggcatcc
     2881 tgctgatcgc gctgacggcc gccctgtact gggtgacgtt ccgcttcgcc accgacatca
     2941 agatcccgct ggtgcgggaa ctggtgctct cggcgcagca aggcgaggca gagcgcgcgc
     3001 gcaagttcgt gcagcagaac ctgaacgcga tggcggtgaa gctgggcgag atgcaggcgc
     3061 agctcgcgcg cctcgacgcc ctgggcgacc gcgtctcggc gatggccggc atccggccgc
     3121 aggaattccg cttcaccgag gccccgggcc tgggcggcgc gcagcccacc gcgctcccgc
     3181 cgcagaacct gtcgctggcg gagttcggcg aaaaactgta cgccctgtcg cgcagcgtgg
     3241 agaaccgcac cgacatgctc ggcgtgctgg agacgcggct tttcgaggaa accatcaaga
     3301 agaaaatgat gccgacctcg ctgccggtca gcgcgcccta caatgcctcc agcttcggca
     3361 tgcgcatcga tccgttcacc ggcatgcagg cgatgcacga gggcatcgat ttcctcgccg
     3421 acctgggttc gccggtcacc gcggccgccg ggggcgtggt caccttcgcc gggttccatc
     3481 cccaatacgg caacgtcgtc gacatcgacc acggcaacga cctggtgacg cgctacgccc
     3541 acctggcgaa gctgctggtc aaggagggcg acatcgtgca acgcctgcac cggctgggcc
     3601 tgagcggcag caccggccgc tcgaccgggc cgcacctgca tttcgaggtg cgcttcaggg
     3661 gcgtcgcgca gaatcccacg cgcttcctct tcgccctgca gggaacgcag caagccgcac
     3721 gttccggccg ctagcggccg cggcggggcg tccgctccgc catgctaaaa ttttgagttt
     3781 gaagggtatt tttgcgcgcg tgcggcagcg cccgcgccac cgatgatcac cagagtcctc
     3841 accaagattt tcggcagccg caacgaccgg ctgctcaggc agtacgcgcg cacggtcgtg
     3901 cgcatcaacc agctcgaagc cggcgtcgcc gcactgtccg acgacgccct gcgcgccaag
     3961 accggcgagt tcaagcggcg cctcgccgag cgcgtcgccg ccgcgccggc ggaagagcgc
     4021 gacgccgccg agggcgaggc gctggaagcg ctgctgcccg aagccttcgc ggtggtgcgc
     4081 gaggccggca agcgcacgct gcgcatgcgc catttcgacg tgcagctggt cggcggcatg
     4141 gtgctgcacg acggcaagat cgccgagatg cgcaccggcg aaggcaagac cctggcggcg
     4201 acgctgccgg cctacctgaa cgcgctcgcc gggcgcggcg tgcacatcgt cacggtgaac
     4261 gactacctcg ccaggcgcga cgccgactgg atgggcaaga tctacggctt cctcgggctc
     4321 agcgtcggcg tcaacgtgcc gcagatggag cccgaggaca agcgcgccgc ctacgccgcg
     4381 gacatcacct acggcaccaa caacgaattc ggcttcgact acctgcgcga caacatggcg
     4441 atgcgcgccg aggagcgctt ccagcgcagc ctgcgctacg ccatcgtcga cgaggtcgac
     4501 tcgatcctga tcgacgaagc gcgcaccccg ctgatcatct ccggccaggc cgaggaccgc
     4561 accgaggtct atgcgcgcat caacgaggtg gcgccgctgc tgacgcggca gaaggacgag
     4621 aagggcgagg gcgatttctg ggtggacgag aagaaccacc agatcacgct gtcggaagcc
     4681 ggccatgcca gggccgagga gatcctggcg cgcgccggct tcctgcccaa gggcgcgagc
     4741 ctgtacgagc ccgcctacat caacctggtg caccacctgt acgcggcgct gcgcgcgcac
     4801 aacctgttcc accgcgacca gcactacgtg gtgcaggacg gcgaggtgat catcgtcgac
     4861 gagttcaccg gccgcctgat gcaggggcgg cgctggtccg acgggctgca ccaggcggtc
     4921 gaggcgaagg agaaggcggc gatccagaac gagaaccaga cgctcgcctc gatcaccttc
     4981 cagaactact tccgcatgta ccgcaagctc gccggcatga cgggaaccgc ggacaccgag
     5041 gcctacgagt tccaggagat ctacggcctg gagacggtga tcgtgccgac gcacaagccg
     5101 atgatccgcg tagaccgcct cgacctggtg taccgcaccg ccaaggaaaa gcacaaggcg
     5161 atcatcgacg acatcaagga ctgccacggc cgcggccagc cggtgctggt gggcaccacc
     5221 tcgatcgagg cctcggagct gctctcgggc ctgctcacca aggaaaagct gccgcaccag
     5281 gtgctcaatg ccaagcagca cgccagggaa gcggagatcg tggcgcaggc cggccggccg
     5341 aagatggtga ccatcgccac caacatggcg gggcgcggca cggacatcgt gctgggcggc
     5401 aacgtcgaga agcaggtcga gctgctcgag gcgaccgggg acgtcgcggc ggcggacaag
     5461 cagacgcgca tcgccaagct cggcagcgag tggcaggcgc tgcacgacca cgtgatccgc
     5521 gaaggcgggc tgcacatcat cggcaccgag cgccacgagt cgcgccgcgt ggacaaccag
     5581 ctgcgcgggc gctccgggcg ccagggcgac ccgggcagct cgcgcttcta cctgtcgctg
     5641 gaggacccgc tgctgcgcat cttcgccggc gagcgcatca acaagatcat ggtgatgctg
     5701 cgcatgcccg agggcgaggc gatcgagcac gccatggtca cgcgctccat cgagagcgcg
     5761 cagcgcaagg tggaggcgcg caacttcgac atccgcaagc agctgctgga gtacgacgac
     5821 gtcgccaacg accagaggaa gacgatctat gccttgcgca acgagctgct cgacgcgcag
     5881 ggcgtcgcgg aacgcattac cgacctgcgc gccggcgtga tcgcggacat gttccgcagc
     5941 tgcgtgccgc cggagagcgt cgaggagcag tgggacatca agggcctgga ggcggcgctc
     6001 aagtcggaac tcaacctggc gctgcccgtc ggccagtggc tggagaagga gcccgagctg
     6061 ggcgacgagg cgctgctcga gcgcatcacg cagaaggcgc acgagtccta ccgcggcaag
     6121 atcccgtccg gggcggagga ttccttccac cagtacgagc gctacatgat gctgcaggta
     6181 ctggacgcgc actggcgcga gcacctggcg gcgctggacc acctgcgcca ggggatccac
     6241 ctgcgcggct acgcgcagaa gaacccgaag caggagtaca agcgcgaggc cttcgagctg
     6301 ttcggcgcca tggtggaggc ggtgaagctc gaggtgacgc gcacgctggt ggcggtcgag
     6361 atccgcgcca gcgacgacgt gccgcaggcc gacgtcggcc agcacgtgga aaacgtgcgc
     6421 ctgcagcatg ccgactacga cacggcgctg gccgcgggcg ccgaagagcg caagccgcag
     6481 ccggcggtgc gccccgcgca gaaggtcggc cgcaacgatc cctgcccctg cggctcgggc
     6541 aggaagtaca agcattgcca cggcaagctg agctagccgc atggcggtga acctgcgcgc
     6601 gccggatgcg gcgcagctcc tcccggtggc cggcatcgag ctcggcatcg ccatggcggg
     6661 cgtgcgcaag gccgagcgca aggacctgct ggtggtgcgc gtcgcgcccg gcaccgcggt
     6721 cgccggcgta ttcacccgca accggttctg cgcggcgccg gtggtgctgg cgaagaagca
     6781 cctgcgcaac ccggtgcacg cgctggtggt caataccggc aacgccaacg cgggcacggg
     6841 cgacgacggg ctgctgcgcg cgctggccgt atgccgcgcc ctggccggcg ccctgcgttg
     6901 ccggccggag caggtgctgc cgttctccac cggggtgatc atggagccgc tgcccgtcga
     6961 gcgcatcgtc gcggggctgc cccgggcggt cgccgacctg cgcgccgcca actgggcgag
     7021 cgcggccgaa gcgatcatga ccaccgacac ggtaccgaag gccgcctcgc gccaggtcag
     7081 gctgagcacg ggcctggcca cggtcaccgg aatcgccaag ggctcgggca tgatccggcc
     7141 ggacatggcg acgatgctgg ggttcgtggc tacggatgcc aagctgccgc gaggctcgct
     7201 gcaacgcctg accaggtccg ctgcggacca atccttcaac gccatcacgg tggacgggga
     7261 cacgtcgacg aacgacgcct tcatatgtct cgccaccgga aaaggcccgg tggcgagatc
     7321 ggggaaagac gaggcggtcc tcgcccgtgc gatcggcgag gttgctcaat ggcttgcaca
     7381 ggcgatcgtg cgcgacggcg agggcgcgac caagttcgtc accgtgcgcg tcgaaggcgg
     7441 tcgcagtgcc gccgagtgcc gcaaggtggc ctacgccatc gcccattcgc cgctggtgaa
     7501 gacggcgttc ttcgcctccg accccaacct ggggcgcatc ctcgcggcaa tcggctacgc
     7561 cggcgtcgcc gggctggata cttcgaaggt ggacctgtac cttgacgagg tgctggtggc
     7621 aaaagggggc ggccggcatg ccggctaccg ggaggagctg ggcgccgcgg cgatgaagaa
     7681 gcccgaattc accgtgcgcg cgctgctcaa ccgcggcaag gcggcggcga ccgtctggac
     7741 ctgcgacctg tcgttcgatt atgtgaagat caacgcggaa taccgctcgt gagccaggac
     7801 atcgacaagc tgctgcagaa gctcgagcaa ctgctgccgc cggcgccgcc gccgaccgac
     7861 tggagcgcga gcatcgcctt ccgctggcgc aagagcgccg ggctgaacgc gcgcggctgg
     7921 ctgcagccgg tgcgccacgt gcaccgcatg cgcctgtcgg acctgcaggg catcggcccg
     7981 cagatcgcca aggtcgagca gaacacgcgc cagttcatcg agggccggcc ggccaacaac
     8041 gtgctgctca ccggcgctcg cggcaccggc aagtcctcga tcgtgaaggg cctgctgaac
     8101 aagtaccaca agcagggact gcgcctcatc gaagtggaga agaacgacct cgtcgacctg
     8161 gccgcgatca ccgacctggt ggccgggcgc gaggagcgct tcctcgtgtt ctgcgacgac
     8221 ctcagcttcc acggcgcgga ggacggctac atcgcgctca aggtggcgct cgacggctcg
     8281 atctccacca cctcggagaa cgtgctgatc tacgccacct ccaaccggcg ccacctgatg
     8341 ccggagtaca tgcaggagaa cctggagacc cgctacgtcg gcgacgagat ccatcccggc
     8401 gagacggtgg aggagaagat ttcgctttcg gagcgcttcg gcctgtgggt gtcgttctac
     8461 ccctttgacc aggacgagta cctggagatc gtggcgcact ggctgacgac cctgggctgc
     8521 ggcgccaggg aggccgaggc ggcgcgcgag gagtccctgc agtggtccct gcagcgcggc
     8581 tcgcgcagcg gccgcgtcgc ctaccagttc gcccgcgact gggccggcaa gcacgccaag
     8641 aagaagtagc gtggcgatcg aagtcgccgc ggcggtgatc cagcgcgccg acggcgcctt
     8701 cctgctggcg cggcgtcccg ccggcaaggc ctacgccggc tactgggagt ttcccggcgg
     8761 caaggtggaa gcgggggagg cggcggagcg ggcgctggtg cgcgaactgc acgaggaaat
     8821 cggcatcgat gcctgcaccg cgtacccctg gattacgcgc gtattcgcgt atccgcatgc
     8881 gaccgtgcgg ctcaattttt tccgcgtgac ggcctggaag ggcgacccgc acccgaaaga
     8941 ggagcaggcc atcgcctggc agcatgtcgg cgcgccgctc gccgagccga tgctgccggc
     9001 caacgcaccg gtgctcgccg cgctggcgct gccgcacgag tacgcgatca cggatgccgg
     9061 gagcagcggc gcgggcgcga tgctgtccag gatccgtccc cgcctcgcgc aaggcctgaa
     9121 gctggtgcaa gtgcgcgaca ggggcttgcc gcagcgcgag gagttcatcg cgacggtggt
     9181 gcgccttgcg caccggcatg gggcgcgggc gatggtgagc ggcgggccgg cgctggggga
     9241 cgggatccat ttcaccgctg ccgaactgat gcggctcgag gagaggccgg cgggcatgct
     9301 ggccgccgcg tcctgccaca ccgcggaaga actgcggcgc gccatggacc tggagcttga
     9361 cttcgcggtg ctcgggccgg tgaagtcgac gccctcgcac cccggcgcgc gacccctcgg
     9421 ctgggatggc ttcgcgagga tcgcgcgcgg cgccagcatc ccggtctacg cgatcggcgg
     9481 catgcagcga tccgacctgg aagccgcctg gcgcgcgggc gcgcacgggg tggcgatgat
     9541 ctccggggcc tggcaggggc tacgccggga cgcttgaccc ttcattgccc cgggatgaca
     9601 ttcccggacg attgccctgc gctgcgcggg gaaaattcgc gccattgaaa actgcggcat
     9661 ggcgggaggc ggcgtgaggc gcaaactcgg acaagtgttg ctggcaggag ctttcctgct
     9721 cccggcgttc gcgctcgagc tttcccttct ggcggcagtg gcgtcggcct gcaattcctg
     9781 caaggcgagc gcgctgcgct agccaggccg cgcgcgccgc ggccttgtag aactttgccc
     9841 gtcaagaaca gttgctgaca tttgcgccgg cgttccggca aatgatgcgc ctgcgcatct
     9901 tcagcaatgc aggcaaacac ggagaacgaa cgatgggagc gatcgagatg tcgatggacc
     9961 tggatttcac gaaaggcgtg cgtgcggcca accccaccct gcccgacgct gcggcattgc
    10021 cgccatccga gatcggcgcg agccggccgg agctgggcgg gaactgcatc ttctacgtgc
    10081 gcgccggcgt ggcgcgcccg gcgtcggcgg atgcgccgat cctggtcgtc gaggacgatc
    10141 cggacacccg gcgcctgctc gagaaagtgc tcgccctgca gggcttcgcg gtgcgcggcg
    10201 cggccgacag ccgcgaattc caggaagcca ttcgccggcc gccgctgccg cagctgatcc
    10261 tgctcgacgt ggaattgccg cgcatcagcg gcttcaagct gctcaccgcg ctgcggcagc
    10321 atccgcagac cagcgccatt ccggtcgtca tggtgacggc gcggaccgcg aacaaggacc
    10381 tgctctacgg cctgtccctc ggggccgacg gctacctgtc caagccgttc accgtggatg
    10441 ggctgcgttc gatgatcgac agggtcctgc cgcgccgcgc ctgagcgccg tgcggcgatc
    10501 cgcgcatgaa ttcgcaggcc accatcctgt acgcgcggct gcacaacgtg ccgccgccgg
    10561 acgacgccac gccgggcacc tgcggcgccg agcttctgca ggaggttcgc gccgtgctgc
    10621 aggaacccat cgcgcggcat gagggcatcg tcgcccagca gcggcccgat tcggtgctgg
    10681 cgatattcgg caatgaccag cgcgaaaagc cggatcacgc gcgacgcgcc atctccgcgg
    10741 cggtgcaggc ggtacacgat ctcgccaggc tcaaccgccg cttcgccgcg cgcatgccgc
    10801 agtcgccgcc gccgcgcctg gcgctcgcca ccggcgtgca cctgggcaac gtcgacgtcg
    10861 cgccggcgcg caacggcagc ggcgccgcgg tgcgcgcgac cggcgaagcg gtcgaggttg
    10921 cgcgcctgct cgagtgcacc gcgccggacc tgggttggag catcgtcgcg tcgcagcagg
    10981 cgcggcgtgc cgccggtctg ccggtcgagt ccggccgctt cggctcggta gcgctgcccg
    11041 acagttcgtt cgtcgacatc gccgagatta ccgggctcgc gccgcagcgc gggcccggca
    11101 acgcggccgc gacctaccag gcgctccgcg aggcgatcgt cttcaatcag cggctgcagg
    11161 accgcccgcg cgacctgtcc ggcgcggccg cacgcgccgc gcgcaacgcc gcgatgcact
    11221 tctcgatcga aggctaccgc atcctgcgca agatcggcga aggcggcatg gcgtcgatct
    11281 accttgcggc gggcccgtcg gaggagccgc aggtcctcaa ggtgatgcgc atcatgagcg
    11341 gcggccagga cgacagcctg cagcgcttca tccaggagtt cgcgctgctg gcgcaggtca
    11401 agcacccgaa cgtcgcgcag atccaccgcc agggcttctc cgcgggccat gcctacatcg
    11461 ccatggagca cttctcgcag ggcgacctgc gcgcgcgcat cgcccagggc atcagccgcg
    11521 cagacgccat cgcctacgtc aagcagaccg cggcggcgct gcaggccatc caccgcgcgg
    11581 gcatcgtgca ccgcgaccta aagccggaca acctgatgct gcgccgggat ggcggtctcg
    11641 cgctggccga tttcggcatc gccaagcacg tggccatgtc gatcaccgac acggcccacg
    11701 gcgaggtggt cgggacgccc tactatctca gcccggagca ggcgatgggg cagcaggtgg
    11761 accagcgctg cgacctgtac agcctcggca tcatcctgta cgagatgctg accggcacca
    11821 aaccctacct tgcgagcacg gcgcaggatc tgctcgacct gcacgtgaac ggtccggtgc
    11881 cgacgctgcg gccgccgcac caggacctgc agccgatcct cgagcggctg atggccaagg
    11941 actgcgaccg gcgctatccg agcgcgcagc atctgctggc ccacctgttc gccctcggac
    12001 tatgagcccg caggcaaccg cgcccgcgat ccgcgtcctg gcggtggacg acgatccgag
    12061 catgcgcagc ctggtctcgc acctgctcgg gcgcgccggc tatgcggtgg acgtggcggc
    12121 ggacggcggc gaggggcttg ccaagatccg cgcccaggcg ccggacctgg tggtgtgcga
    12181 catccagatg ccgaacatgg acggcttcgc gatgctggat gccgtgcgct ccgacacggc
    12241 gaccgcgtcg ctgccgttcg tgctgctcac cgcgctcacc gatcgcgaca gcgtgcggcg
    12301 cggcatgcgg ctgggcgccg acgatttcct ctccaagccg gtgcgcgggc acgagctggt
    12361 cgaggcggtg agcgtcgccc tcgacgggcg ccggcggctc agcgggctgg tgtccggccg
    12421 cgcgctgccc gagcagcggg agctgcgcga gcgctacgaa aggaagatcg acggcggcga
    12481 gccgcaggcc gcgcgggaga ccagctttgc cggcatgaca gggcgcatgc tcacgcagac
    12541 ggtgctgttc tcggacatcc gcggcttcac ctcgatgtgc gagcgcctgc cggtgccgga
    12601 gatcgccgaa ctgctgagcc gctacctgcg cgaggcctgc cggccgatcc tgcaggagcg
    12661 cgggcgcatc atgaagatca tgggcgacgg cctgatggcg atcttcggcc aggatgcgcc
    12721 gcacgacgtt gcggcacacg ccgcggcggc gctgcgcgcc ggcgagcgga tcatcgacgt
    12781 ggcgcaggaa ttccggcgct ggatcgcgtc gcgcttcgac ctgcccggac tgccgccgtt
    12841 cgatgccggc gtcggcatcc acaccgggca agtgatgctg ttccagctgt cggtcggcgg
    12901 cgcgggcgac ctgaccgcgg tgggcgacac ggtgaacgtc gccgcccggc tggaagccaa
    12961 atccaaggag ctgggctggc cgatcgtcgc cagcatggcg accattgagc acgcgggagc
    13021 tcacgcacgg gtcggcgaga tccgcgaagt ggtgctggcc ggccgcgatg cgcgcatcca
    13081 ggtggggcgc atcaacctgc tcgacgcggg cgcggcggag ccgcgcgtgc agctgccgct
    13141 caccgccggg atcgaggtcg tgctcgacga gagcgcccgc gcgacggccg aagcggccaa
    13201 gcaggcgctc gaccgcacgc tgcacgccat cggcgagcag ctgcaccagt accccgcgac
    13261 gtccgaagcg gagccggtga tccacggcta ccgggtcctg gcgaagatcg gcgaaggcgg
    13321 catgtcgagc gtgttcctgg cagaggagca ggcgcgccac cgcaaagtgg tgctcaaggt
    13381 gctcaagggc aagcgcgtgg acgacgacgg cctgtggaag cgctttttcc aggagtgcgc
    13441 catcctgtcc tcgatcaagc acgaccacgt ggtgcgcatc tacgatcagg gattcggcga
    13501 cgagctcgcc tacatcgcga tggagcacct ggcgggcggc agcctgcgcg agctgatgga
    13561 ccgcggcctg tcgccgcgcc aggccctgtc gctgctgtcg caggcggcga gcggtctcgc
    13621 cgagatccac cgctgcggca tcgtgcaccg cgacatcaag ccggccaacc tcatgctgcg
    13681 cgaggagggc gtgctggtgc tgaccgattt cggcgtggcg aaaaggctgg aaaccgcggc
    13741 gggacagacg atgcacggcg agatcctcgg cacgccctat tacatctcgc ccgagcagtc
    13801 gcagggcggc gcggtgaccc cgcgcaccga cctgtacagc ctgggggtga tcttctacga
    13861 gatgctgacg ggcgcgcgac ctttcgtcgg cgacacgctg ctcgagatcc tcgcgcagca
    13921 cacggcggcg ccgatcccgc gcctgcccgc cgcgctcgcc gcctaccagc ggctggtcga
    13981 cggcatgctg gccaagcgcc ccgcggagcg cttcgacggc gccgaggcgg tgctcgccga
    14041 gatcgaccgc gtgtggacgc agcacgcgct gctcaaggcg tcgcgcgaag cggccgcggc
    14101 gcactagggc gcacgggaat ttgcagtgcc tgacaaagtc cttgcacagc atgccaatcg
    14161 cgcgcgcggc gctcgctaca ttgcctgcat tgcacagcga tgaccccgga catgacgcaa
    14221 cccgacaacc actactcgat cgacgtcttc ggcttcaacc agactgagcg actgatgctg
    14281 tccagcatct tcggcctggc cgcccggcgc gacccgagct tcgtgcaacg tgacgccggc
    14341 gccacgcggc cggcggacct gtatctcgtg gatggcagcg accctgccgc gatgtccgag
    14401 ttccacgccg tgcgcgcgcg ctacccggcg ccgacggtgc tggtcggcga cgcggatccg
    14461 cagagcggcc tgccggccat gccgcgccca attcagtggg cgcgcctgct gcagagcttc
    14521 gacgacgcgg tgctgggcga cgcgcagccg gccccggcga attccgccgc ggcaggcgtt
    14581 ttcgacaaga ccatggtcct gcgccgcacc ggcgccccgg cacccgccgc ggcgacaccg
    14641 gcggcgccga tgcgtcctgc ggcggcggcg cgcccgcagc ccaggcccaa gcagagcacg
    14701 gacaccgtcc tggtggtcga cgacaacgcc accgtgcgca agttcatgga agccaagctc
    14761 gcgccctacg gcttcagcgc cgatttcgcc gagaccggcg agcaggcggt cggcctgacc
    14821 ggcaccaagg agtacacctg cgtgttcctg gacgtggtgc tgccgggcat cgacggctac
    14881 caggtgtgca agctgatcaa gtccaacaag caggcggtga agcgcaccgc ggtggtcatg
    14941 ctgaccagcc gcagctcgcc gttcgacaag ctgcgcggct cgctcgccgg ctgcgacgaa
    15001 tacctgacca agccggtcga cgagaaccag ctgctcgagg tgatcgcgaa gttcctgccc
    15061 tcggcgcgcg ccgccgcagc ctaacggccc ccgcattcga caaggaatcc caatgagcaa
    15121 atcgatcctg gtggtggacg acacgcgctc catgcgcaag atggtgatga ccgtgctgca
    15181 ggcggccggc tacagcgtgg aggaggccgg cgacggcgcc gaggcgctgg agaaggcgca
    15241 gacccgcgtg ttcgacctgg tggtcacgga ccacaacatg ccgcgcatgg acggcgtcac
    15301 gctggtgggc gcgctgcgca agctgcccgc gtacgacgag gtcgcgctga tcgtgctgtc
    15361 gacggaaacc ggccccgagc tcaaggcgaa ggggcgcgcg gcgggcgcca ccggctggat
    15421 ggcgaaaccc ttcgaccccg agaagatgct gggcatcgtc gggcagttca tcggatgagc
    15481 gtcgctgccg caaaagcgct cggcgacgcc tcggccttcc acgccctgtt cttcgaggaa
    15541 gcggacgagc acctcgccgc gatcgaggaa atcctgctgc gcatcgaccc ggcggcggcg
    15601 gcgggcgagg acctgaacgc gatcttccgc gccgcccact ccatcaaggg cagcagcgcg
    15661 atgctgggct ttcccgagat cgcctcgctc acccacgtcc tggagaacct gctcgatatc
    15721 ctgcgcaagg gcgagcgggc gctgacgcgg gaggacgtgg atgccctcct gcgcgcgggc
    15781 gacgtggtga agatgcagct cgcattccgc aggggcgcgg ccgccgcggc gcccgacgtg
    15841 gacggcacgg agaacgagct gcgcgcgctg gccgtcgcgg cgccggcgac gctgcggcgc
    15901 tatgcggtgc ggctcggccc gctcgccgcg ccgatcgagg ccgccgagct cgacaccatg
    15961 ctctgcggcc tgtccggcat gggcgaggtg cacgatcgcc atctggaaaa tattgccggc
    16021 ggcgagatcc gcttcgggct ggcgctcgcg ggcggcgagc acgatctccg gagcgtgctc
    16081 acgctggtgg tcgcgcccga gctggtgcat atcagcgccg gcgatgcggc cgccagcccc
    16141 gatgcgccgg cggcaacgcc gccggcggcc acggacgacg agctgttcgt cgacccggcg
    16201 cagtggcgcc gccgcgcgaa tccggagccg acggacgtcg atctgttcgt gacgccgcag
    16261 gcgtggcgcg ccaggcgcgg cgccggccgc cgcgccggcg acgacccggt cgcgcccgcc
    16321 gagcgcgaag gccggcgtga agccgatcgc caggcgcccg ccgtgcaggc cgacgccacc
    16381 tcgatccggg tgagcgtgga gaagatcgac cagctggtca acctggtggg cgagctggtg
    16441 atcaccgaag ccatgctggc gcagcagcac ggcggattgc agaaagccgg cgggcacgcc
    16501 gagctggcgg gactcggcga cctggcgcgc cacacgcgca acctgcagga agccgtgatg
    16561 gcgatccgca tggtgccgat ttccgccgtg ttctcgcgct ttccgcgcct ggtgcgcgag
    16621 ctggcgcagc gcctgggcaa ggaggtcgag gtcaagctct cgggcgaggc gaccgaactc
    16681 gaccgcggcc tgaccgagaa gctcatcgat ccgctcacgc acctggtgcg caacgcgatc
    16741 gaccacgggc tggagcggcc ggaggtgcgc ctcgcggccg gcaaatcgcg cggcggcacg
    16801 gtgtcgctgg cggccagcca gcgcggcgga cgcatcgtca tcgaagtgcg cgacgacggc
    16861 cgcgggctcg atcgcgcgcg catcctcgcg cgcgccgccg agcgcggcgc atcgatcgca
    16921 gcggacgcgc cggatcacga agtctggcag ctgatcttcg aagccggatt ctcgactgcc
    16981 gacagcgtga ccgacgtatc cggccggggc gtcggcatgg acgtcgtgcg ccgcaatatc
    17041 cagtcgctcg gcggtaccgt ggacctcgcc tccagcgcgg gaaccggcac caccgtgacc
    17101 gtcagcgtgc cgctcacgct cgccatcatc gaggcgatga cggtggccgc cggcgggcag
    17161 acctacgtgg tgccgctcgc ctcggtggtg gagtcgcggc gcgtgggcag ggacgaaatc
    17221 cgcggcatcg ccgggcgggg ccgcacccta cgcgtgcgcg acgactatct gccggtgcag
    17281 cggctggcgg ggcagtgcgc tcgcccgcaa gacgaatcgg gcgacggcgg cattgccgtg
    17341 atcgtggaag cggacggcgc gaaggcggcc ttgctggtcg acgagctgat cggccagcag
    17401 caggtggtgg tgaagagcct ggaggcgaat ttccgcaggg tcccgggcat cgcgggcgcc
    17461 accatcatgg gcgacggcaa ggtcgcgctg atcctcgaca tgacccacct ggtgaaccag
    17521 gcaggacgca gccaagcccc ccttcactga ctggcgcggg cggcttttca ctcccttgcc
    17581 ggcggcaaac agtacatgac atttcccgag gcacgaaagc acaaagtgcc cgccatggac
    17641 gacttgagga gaagccacat gcaggcgatt ggcagcaacg cagtgtcccg cgccgcgccg
    17701 gcgcagcagc cgggcggcac ggccgacgcc gaggggcggg agttcctgac gttccgcctc
    17761 ggcgccgagg a
//
LOCUS       DASRIE010000251         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2071604, whole genome shotgun sequence.
ACCESSION   DASRIE010000251 DASRIE010000000
VERSION     DASRIE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071604"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VFB53_10940"
     CDS             <1..1431
                     /locus_tag="VFB53_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HZN87286.1"
                     /translation="ILIAALGGEGGGVMADWLMHAATACGYPAQATSIPGVAQRTGAT
                     TYYLEIFPARRADLGGREPVMSLTPSPGNVDVMVASELLEAGRAMQNGFVTPERTTLV
                     ASTHRIYATVEKMQMADGRFSSERVLAAAKELSRQAVLFDMRRLAEESGTVINAVLFG
                     AMAGSGALPLPREACEAAIRRAGKGAQASLRGFAAGYDIAAGARQAPAAAPRPLPPGT
                     REILDLGAARLRDYQGEAYARLYLERIAALGDAEPKLIGETARQLALWMSYEDIIRVA
                     DLKTRRARFARVRREAGARDGEPLVVIDYLKPGLEEVASLLPAALGRRLLAWAERKGK
                     LDAYNVGMHVKTSGIGGYLLVRALAWLKPWRPRSLRYAEEQALIERWLAAVRDAASRS
                     LPLALEVVECAGLLKGYGETHRRGRSSFLAILDALVENPATADPAAQAAAIRRAREAA
                     LSDPEQKALEKTLGRPVTWLRAEKTR"
     gene            1444..2301
                     /locus_tag="VFB53_10945"
     CDS             1444..2301
                     /locus_tag="VFB53_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN87287.1"
                     /translation="MSSIADPAFLSREYNNRELFPDHPRHFARWQESSARARSTMSCH
                     LDLAYGDAPAETLDLFPSRKGDGSLLVFIHGGYWRSLDKRDFSYLAPAWVDAGVSLAV
                     VNYALCPAVTIERIVRQVLAACAWLYRNAERYGMDEERIFVSGHSAGGQLAAMTLAAL
                     WPVVDGSLPRDLVKGALAVSGVYDLRPLTQVGWLSGDLRLDEAAAVKVSPAFLPPATR
                     APLYLAVGGLESSEFKRQNALLAQRWKAVLGGDIGMPGRDHFTVVDGLAEPASALFAG
                     ARRMMGLGR"
     gene            2298..2936
                     /locus_tag="VFB53_10950"
     CDS             2298..2936
                     /locus_tag="VFB53_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN87288.1"
                     /translation="MREFLAPGRYVDSAHPAVAEFARRHARGASDLERAVSLYYAVRD
                     GIRYNPFLDFSRDTAFQGSVCLQTGEGFCVGKAALLAACARAVGIPARVGFADVKNHL
                     TTPRLAERMGTDLFVYHGYTELWLEGRWVKATPAFNLELCRKFRVKPLEFDGRSDSIF
                     HPFDADERRHMEYLRERGSFADVPVEEIQRAFRDTYPALYGLGKDAARETFA"
     gene            complement(2933..>3636)
                     /locus_tag="VFB53_10955"
     CDS             complement(2933..>3636)
                     /locus_tag="VFB53_10955"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZN87289.1"
                     /translation="VTIPSPTMLHFRGGRGAISREAYPDMEGFYEDVARCYREELRQL
                     ADAGCRYLQLDDTNLAYLCDPKMREGARQRGDDPDALPRRYARLINSAIAGRPQDMVV
                     GVHLCRGNFQSAWAAEGGYEPVAEVLFNELQVDGYFLEYDDARSGDFSPLRFVPKGKI
                     VVLGLVTTKLGSLEAKDDLKRRIDQAAKHVPLEQLCLSPQCGFSSTVHGNALAVEAQA
                     AKIRLVIETAREVWG"
BASE COUNT          496 a         1308 c         1306 g          526 t
ORIGIN      
        1 attctcatcg ccgcgctcgg cggcgaaggc ggcggcgtga tggccgactg gctgatgcat
       61 gccgccaccg cctgcggcta tcccgcccag gccacctcga tccccggcgt cgcgcagcgc
      121 accggcgcca ccacctatta cctggagatc ttccccgcgc ggcgcgccga tctcggcggc
      181 cgcgagccgg tgatgtcgct cacgcccagc ccgggcaacg tggacgtgat ggtggcttcc
      241 gagcttctgg aagccggccg cgccatgcag aacggcttcg tcacgcccga gcgcaccacg
      301 ctcgtcgcct cgacccaccg catctacgcc accgtggaga agatgcagat ggccgacggg
      361 cgcttctcca gcgagcgcgt gctggcggcg gccaaggagc tctccaggca ggcggtgctg
      421 ttcgacatgc gccggctggc cgaggagagc ggcacggtca tcaacgccgt cctgttcggc
      481 gccatggcgg gctcgggcgc gctgccgctg ccgcgcgagg cctgcgaagc cgcgatccgc
      541 cgcgccggca agggagcgca agcgagcctg cgcggctttg cggccggcta cgacattgcc
      601 gccggcgctc gccaggcgcc ggccgcggcg ccgcggccgc tgccgcccgg gacgcgcgaa
      661 atcctggacc tgggcgcggc acgcctgcgc gactaccagg gcgaggccta tgccaggctg
      721 tatctcgagc gcatcgccgc gctgggcgac gccgagccga agctcatcgg cgagaccgcg
      781 cggcagctcg cgctgtggat gagctacgag gacatcatcc gggtcgccga cctgaagacg
      841 cgccgcgcgc gctttgcgcg ggtgcgcagg gaagccggcg cgcgggacgg cgagccgctg
      901 gtggtgatcg actacctgaa gcccgggctg gaggaagtcg cttcgctgct gccggctgcg
      961 cttggccggc ggctgctcgc ctgggccgag cgcaagggca agctcgatgc ctacaacgtc
     1021 ggcatgcacg taaagacctc gggcatcggc ggctacctgc tggtacgcgc cctggcctgg
     1081 ctcaagccct ggcggccgcg cagcctgcgc tacgccgagg agcaggcgct gatcgagcgc
     1141 tggctggccg cggtgcgcga cgccgcatcg cgcagcctgc cgctggcgct ggaggtggtc
     1201 gaatgcgccg ggctgctgaa gggctacggc gagacgcacc ggcgcggcag gagcagcttc
     1261 ctcgcgatcc tcgacgccct ggtggagaac cccgccaccg ccgatcccgc ggcgcaggcg
     1321 gcggcgatcc gccgggcccg cgaagccgcg ctgtccgatc ccgagcagaa ggcgctggaa
     1381 aagacgctcg gccggccggt gacctggctg cgcgccgaaa agacccgcta gaatccgccg
     1441 ctcatgtcca gcatcgccga cccggcgttc ctcagccgcg agtacaacaa ccgcgagctg
     1501 ttccccgacc acccgcggca tttcgcgcgc tggcaggagt cctcggcgcg cgcgcgctcg
     1561 accatgtcct gccacctgga tctcgcctac ggcgacgcgc cggcggagac gctggacctg
     1621 ttcccgtcgc gcaagggaga cggcagcctg ctggtattca tccacggcgg ctactggcgc
     1681 tcgctcgaca agcgggactt ctcctacctc gcgcccgcct gggtggacgc gggcgtgtcg
     1741 ctggcggtgg tcaactacgc gctctgcccg gccgtgacga tcgagcgcat cgtgcggcag
     1801 gtgctcgccg cctgcgcctg gctgtaccgc aacgcggagc gctacggcat ggacgaggag
     1861 cggattttcg tcagcgggca ctcggccggc gggcagctgg cggcgatgac gctcgccgcg
     1921 ctgtggccgg tggtcgacgg ctcgctgccc agggacctgg tcaagggcgc gctcgcggtg
     1981 agcggcgtct acgacctgcg gccgctcacc caggtcggct ggctgagcgg cgacctgcgc
     2041 ctggacgagg cggcggcggt caaggtttcg cccgccttcc tgccgccggc gacgcgcgcg
     2101 ccgctgtacc tcgcggtggg cgggctggag agctccgagt tcaagcgcca gaacgcgctg
     2161 ctcgcgcaac gctggaaggc ggtcctcggc ggcgacatcg gcatgcccgg ccgcgaccac
     2221 ttcaccgtgg tggatgggct ggcggagccg gcgagcgccc tgtttgccgg cgcgcggcgc
     2281 atgatggggc tgggccggtg agggagtttc tcgcccccgg gcgctatgtc gacagcgccc
     2341 acccggcggt ggcggagttc gcgcgccggc atgcgcgcgg cgcctccgac ctggagcgcg
     2401 cggtgtccct gtactacgcc gtgcgcgacg gcatccgcta caacccgttc ctcgactttt
     2461 cgcgcgacac cgcgttccag ggcagcgtgt gcctgcagac cggcgagggc ttctgcgtcg
     2521 gcaaggcggc gctgctggcg gcctgcgcgc gcgccgtcgg catcccggcg cgcgtgggct
     2581 tcgccgacgt gaagaaccac ctcaccacgc cgcggctcgc cgagcgcatg ggcaccgacc
     2641 tgttcgtgta ccacggctac accgagctgt ggctggaggg caggtgggtg aaggcgacgc
     2701 ccgcgttcaa cctcgagctg tgccgcaagt tccgcgtcaa gccgctggag ttcgacggcc
     2761 gcagcgactc gattttccat cccttcgacg ccgacgagcg gcgccacatg gaatacctgc
     2821 gcgagcgcgg ctcgttcgcc gacgtgccgg tggaggagat ccagcgcgcg ttccgcgaca
     2881 cctatccggc gctctacggc ctgggcaagg acgccgccag ggaaaccttc gcctagcccc
     2941 agacctcgcg ggcggtctcg atcaccaggc ggatcttcgc cgcctgggcc tccacggcga
     3001 gcgcgttgcc gtgcaccgtg ctggagaagc cgcactgcgg cgacaggcac agctgctcca
     3061 gcggcacgtg cttcgccgcc tggtcgatgc gccgcttgag atcgtctttc gcctcgaggc
     3121 tgccgagctt ggtggtcacc aggcccagca ctacgatctt gcccttgggc acgaagcgca
     3181 ggggcgagaa atcccccgag cgcgcgtcgt cgtattcgag gaagtacccg tccacctgca
     3241 gctcgttgaa cagcacttcc gccaccggct cgtagccgcc ttcggcggcc caggcgctct
     3301 ggaagttgcc gcggcacagg tgcacgccga ccaccatgtc ctgcggcctg ccggcgatgg
     3361 ccgagttgat caggcgcgcg tagcgcctgg gcagcgcgtc cgggtcgtcg ccgcgctgac
     3421 gcgcgccctc gcgcatcttc gggtcgcaca ggtaggcgag attggtgtcg tcgagctgca
     3481 ggtagcggca acccgcgtcc gcgagctggc gcagctcctc tcggtagcag cgcgcgacgt
     3541 cctcgtagaa gccctccatg tcgggatagg cctcgcggct gatggcgccg cgcccgccgc
     3601 ggaaatgcag catcgtgggc gacgggatgg tgacct
//
LOCUS       DASRIE010000252         7574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2600069, whole genome shotgun sequence.
ACCESSION   DASRIE010000252 DASRIE010000000
VERSION     DASRIE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600069"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..512)
                     /locus_tag="VFB53_10960"
     CDS             complement(159..512)
                     /locus_tag="VFB53_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HZN87290.1"
                     /translation="MEIVVALGIGVLAGSGVWLLLRPRTFQVIVGLSLLSYAVNLFIF
                     VMGWVRGGLAPVVAEAARGATPDPARYADPLPQALVLTAIVIAFATTALLLVVLLAAR
                     GLTGTDHVDGREPGE"
     gene            complement(512..3382)
                     /locus_tag="VFB53_10965"
     CDS             complement(512..3382)
                     /locus_tag="VFB53_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HZN87291.1"
                     /translation="MADEVRLLLLLALPFAGSLAAGMLPANARNAEAWLSGAVALAGV
                     ALAASSYGEMADGRVVRAAWDWLPAFGVTLALRMDGFAWMFSLLVTGIGFLVVLYARY
                     YMSPKDPVPRFFAFFLAFMGSMLGTVLAGNLIQLVFFWELTSLFSFLLIGYWHQNAAA
                     RDGARMALILTSIGGLCLFGGALLLGRIAGSYELEAIVAAGERIRADDLYVPALALVA
                     VGALTKSAQFPFQFWLPQAMAAPTPVSAYLHSAAMVKLGVFLLARLWPAMAGTDAWIW
                     IIGTAGLVTLVLGAYIAIFQHDLKGLLAYSTISHLGLITVLLGLNSPLALVAAIFHMM
                     NHATFKASLFMAAGIIDHESGTRDIRRLSGLYRLMPITATLAMVAAAAMAGVPLLNGF
                     LSKEMFFSEALQVEGPVRVLNLALPFVATIWGMFSVAYSLRFIHGAFFGPPPVGLPRT
                     PHEPPHWMRFPVELLVAACLVVGILPALTVGPILELAVRAVLGPQTPAYSLAVWHGFT
                     TPLVMSLFALGGGAVLYLLLQKFLARGEERTPLMPPVEGRRIFDRVLVTVSWQWARAL
                     EALLGTRRLQAQLGILVLLAVAAGLWPVWQRGLAFGPLAPSPAEPGFVLMWAAGIACA
                     LGAAWQAKFHRLVTLVLAGGAGLVVCLTFVWLSAPDLALTQLLVEIVTTVLLLLGLRW
                     LPKRIPFRWTLAGARAALPRRARDLAIACASGGGLGLAAYAVMTRPLPDHAISRFFIE
                     RAWPEGGGSNVVNVIIVDFRAFDTLGEIAVLGVVAVAVYSLLRRFRPAQESTEPLQQH
                     VLQSTAAAAEDLAVPAVIMRAMFPVTALLSAYLLLRGHNLPGGGFVAGITLAVGIILQ
                     YMAGGTRWTEERLRMRPLRWMGLGLLAAAGTGAAALLFRHEFLTSHVSHLALPFLGEL
                     HVPTAFFFDLGVFALVVGATALILIALAHQSTRAHRAPGERR"
     gene            complement(3383..5524)
                     /locus_tag="VFB53_10970"
     CDS             complement(3383..5524)
                     /locus_tag="VFB53_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZN87292.1"
                     /translation="MSLHLEHLNPEQLAAVTLPDEHALILAGAGSGKTRVLTTRIAWL
                     VKGGRVAPSGILAVTFTNKAAREMLTRIGAMMTVNPRGMWVGTFHGLCNRMLRAHARE
                     AGLPAAFQILDSQDQLAAIKRLLKGMNVDEERFPPREVQYFINDRKEEGVRARDVPPA
                     DDNTRRFAELYAAYDEQCQRDGVVDFPELLLRSYELLTRNEVLREHYARRFRYILVDE
                     FQDTNRLQYRWLKLFYSRENYLFCVGDDDQSIYTFRGANVGNMAEFEREFKVRKVIRL
                     EQNYRSHGNILDAANALIANNRNRLGKNLWTSAGKGEPVRVFEGESDNDEARWIVEEV
                     QSLARDGTRLSHIAVLYRSNAQSRVVEHALFSQGIPYRVYGGLRFFERAEIKHALAYL
                     RLIATPEDDNSFLRVANFPPRGIGARTIEQLQEAAKRAGASLYKVADGKAAVFRKLIE
                     DLKSETQGLPLKEMIEAVIAKSGLVEHYKNEREGADRVENLEELVNAAAAFTEEERVS
                     ESGEAVDPLTAFLTHAALEAGEHQAGDGQEALQMMTVHSAKGLEFDAVFITGLEEGLF
                     PHEQSVSERDGLEEERRLAYVAITRARQRLYLSHAQTRMLHGQTRYNIPSRFLEEIPQ
                     QLLKWLTPRFSRQKMLQAGGWAAPRPAPSARPPRDVGGFRIGQNVRHPKFGPGVIVDA
                     EGQGTDARVQVNFGGQGVKWLAVAVAKLQAA"
     gene            complement(5545..6417)
                     /locus_tag="VFB53_10975"
     CDS             complement(5545..6417)
                     /locus_tag="VFB53_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZN87293.1"
                     /translation="MKALLVAVLLALTSAAALAQGGDTQAAALVDGVQMPAWRERDGK
                     RTPLVPGMELRAGDRIISGKDARVLVKLSEGSVVKLGENGALRLAEMRPTRELFRAAL
                     QVLEGAFRFTSDLAAKARKREVTVRVEQVTIGIRGTDFWGRGRGERQIVCLIEGAIQV
                     GAPGEPPLTLDRPRQFYQRDKGRAQPVGFVEASQLEQWSREVEIEPGRGALRSGGRFG
                     VQLAAGDTREALAQVQEQLADAGYAAEIAPSADARQAPYVVRIRHLPSREEAQALARR
                     LDGQFGIADPVVLQ"
     gene            6457..>7574
                     /gene="leuS"
                     /locus_tag="VFB53_10980"
     CDS             6457..>7574
                     /gene="leuS"
                     /locus_tag="VFB53_10980"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZN87294.1"
                     /translation="MQETYDPAAVEKEAQAFWAARDSFRSVEDRARPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYHRMRGYNVLQPMGWDAFGLPAENAAMANRVPPARWTY
                     DNIAYMKRQLQGLGFALDWSRELATCRPDYYRWNQWLFTRLFKKGLAYKKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGALVEKREIPMYFLRITAYAEELLAALEKLPGWPEQVKL
                     MQKNWIGKSDGVRIGFPYELNGKKETLWVFTTRADTLMGATFMAVAAEHPIAVFASQK
                     DAKLKAFVEECRKGAVMEADLAQIEKKGMPTGLHALHPITNERIPIWVGNYVLMSYGE
                     GAVMGVPAHDERDFAFALKYKLPIKPVIRHPLGDTVPAP"
BASE COUNT         1252 a         2674 c         2613 g         1035 t
ORIGIN      
        1 cagcgcgagc gcaacgctcg cgagcgcgag cagcagcgcc gcgcccaggc tgatcgccgc
       61 cttgagcgag tggcgccgct cgtcgaggag cagcatcgcg gcggcggcgg cgagcggcag
      121 cacgaccggc accaccacga ggtggtcggg cgcggcgctc attcgcccgg ctcccggcca
      181 tcgacgtggt cggtgccggt gagcccccgg gccgcgagca gcacgaccag cagcagcgcg
      241 gtggtcgcga acgcgatcac gatcgcggtc agcaccagcg cctgcggcag cgggtcggcg
      301 tagcgcgcgg gatcgggcgt cgcgccgcgc gcggcctcgg cgacgaccgg cgccagcccg
      361 ccgcgcaccc accccatgac gaagatgaag aggttgaccg cgtaggaaag gagcgaaagg
      421 ccgacgatca cctggaaggt gcgcggcctg agcagcagcc acacgcccga gcccgcgagc
      481 acgccgatgc cgagcgccac gactatctcc atcagcgcct ctcgcccggc gcgcgatgcg
      541 cgcgggtcga ctggtgcgcg agcgcgatca ggatgagcgc ggtggcgccc accaccagcg
      601 cgaacacgcc gaggtcgaag aagaacgcgg tgggcacgtg cagctcgccc aggaacggca
      661 acgcgaggtg cgagacgtgc gaggtgagga actcgtgccg gaacagcaag gccgcggcgc
      721 cggtgccggc ggcggcgagc agcccgagcc ccatccagcg cagcggccgc atcctcagcc
      781 gctcctcggt ccagcgcgtc ccgcccgcca tgtactgcag gatgatgccc accgcgaggg
      841 tgatgccggc gacgaagccg ccgccgggca ggttgtgccc gcgcagcagc aggtatgcgc
      901 tcagcagcgc ggtcaccggg aacatggcgc gcatgatgac cgccgggacc gccaggtcct
      961 cggccgccgc cgcggtgctc tgcagcacgt gctgctgcag cggctcggtg ctctcctgcg
     1021 ccgggcggaa gcgccgcagc agcgaataga ccgcgaccgc gaccacgccc agcaccgcga
     1081 tctcgcccag cgtgtcgaag gcgcggaagt cgacgatgat cacgttgacc acgttgctgc
     1141 cgccgccctc gggccaggcg cgctcgatga agaagcgcga gatggcgtgg tcgggcagcg
     1201 gccgcgtcat caccgcatac gccgcgagcc cgagcccgcc gccgctcgcg caggcgatcg
     1261 ccaggtcgcg cgcgcggcgc ggcagcgcgg cgcgcgcccc ggcgagcgtc cagcggaacg
     1321 ggatgcgctt gggcagccag cgcagaccga ggagcagcag cacggtggtg acgatctcca
     1381 ccaggagctg cgtcagcgcg aggtccggcg ccgaaagcca gacgaaggtc aggcacacca
     1441 ccaggcccgc gccgccggcg agcaccagcg ttaccagccg gtggaacttc gcctgccacg
     1501 ccgcgcccag ggcgcacgcg atgccggccg cccacatgag gacgaaaccg ggttccgccg
     1561 ggctcggcgc cagcggaccg aacgccaggc cgcgttgcca taccggccac agccccgcgg
     1621 ccacggcgag cagcaccagg atgccgagct gcgcctgcag gcggcgggtg ccgagcaaag
     1681 cctcgagcgc gcgcgcccat tgccacgaca ccgtgacgag cacccggtcg aagatgcgcc
     1741 ggccctcgac cggcggcatg agcggcgtgc gctcctcgcc gcgcgcgagg aacttctgca
     1801 gcagcaggta gagcacggcg ccgccgccca gggcgaacag gctcatcacg agcggcgtgg
     1861 tgaagccgtg ccacaccgcc aggctgtacg cgggggtttg cggcccgagc accgcgcgca
     1921 ccgcgagctc gaggatcggc cctacggtga gcgccggcag gatgcccacc accaggcagg
     1981 cggcgacgag cagctcgacc gggaagcgca tccagtgcgg cggctcgtgc ggcgtgcgcg
     2041 gcagcccgac cggaggcggc ccgaagaagg cgccgtggat gaagcgcagc gagtacgcaa
     2101 cgctgaacat gccccagatc gtcgcgacga acggcagcgc caggttcaga acgcgcaccg
     2161 ggccctcgac ctgcaacgcc tcggagaaga acatttcctt ggaaaggaag ccgttcagca
     2221 gaggcacgcc agccatggcc gcggccgcga ccatcgcgag cgtcgcggtg atcggcatca
     2281 gccggtacag gccggatagc cgccggatat cgcgcgtgcc gctttcgtgg tcgatgatgc
     2341 cggccgccat gaagagcgac gccttgaagg tggcgtggtt catcatgtgg aagatcgccg
     2401 cgaccagcgc gagcgggctg ttcagcccga gcagcaccgt gatcaggccg aggtggctga
     2461 tcgtcgagta ggcgagcagg cccttcaggt cgtgctggaa gatcgcgatg taggcgccca
     2521 gcaccagcgt caccaggccc gcggtgccga tgatccagat ccaggcgtcg gttcccgcca
     2581 tcgccggcca gaggcgcgcc aacaggaaca cgccgagctt caccatcgcc gccgagtgca
     2641 gataggccga gaccggcgtc ggcgcggcca tcgcctgcgg cagccagaac tggaacggga
     2701 actgcgcgct cttggtgagc gcgccgacgg ccaccagggc gagcgccggc acgtacaggt
     2761 cgtcggcgcg gatgcgctcg ccggcggcca cgatggcctc gagctcgtag ctgccggcga
     2821 tccgcccgag cagcagcgcg ccgccgaaca ggcatagccc gccgatcgag gtgaggatga
     2881 gcgccatgcg cgcgccgtcg cgcgccgcgg cgttctggtg ccagtagccg atgagcagga
     2941 acgagaacag gctcgtcagc tcccagaaaa ataccagctg gatcaggttg ccggcgagca
     3001 ccgtgccgag catcgacccc atgaaggcga ggaagaaggc gaagaagcgc ggcaccgggt
     3061 ccttgggcga catgtagtag cgcgcgtaga gcaccaccag gaacccgatg ccggtgacga
     3121 gcaggctgaa catccaggcg aagccgtcca tgcgcagcgc gagggtcacg ccgaacgccg
     3181 gcagccagtc ccacgcggcg cgcaccacgc ggccatccgc catctcgccg taggagctcg
     3241 cggcaagcgc gacgcccgcc agagccaccg cgccggaaag ccaggcttcg gcgttgcggg
     3301 cattcgcggg cagcattccc gccgccaggc tgccggcgaa cggcagcgcc aacagcagca
     3361 gcagccggac ttcgtccgcc acctaggcgg cctgcagctt ggccaccgcc accgccagcc
     3421 acttcacgcc ctggccgccg aaattcacct gcacgcgcgc atccgtgccc tggccttcgg
     3481 cgtcgacgat cacgccgggt ccgaacttgg gatgccgcac gttctgcccg atgcgaaagc
     3541 cgccgacgtc gcgcggcggc cgggccgagg gcgccggccg cggcgccgcc cacccgcccg
     3601 cctgcagcat cttctgccgc gagaagcgcg gcgtcagcca cttgagcagc tgctgcggga
     3661 tctcctcgag gaagcgcgac gggatgttgt agcgcgtctg gccgtgcagc atgcgcgtct
     3721 gcgcatgcga caggtacagc cgctggcgcg cgcgcgtgat cgcgacgtag gcgaggcggc
     3781 gctcctcctc cagcccgtcg cgctcgctca cgctctgctc gtgcgggaac aggccttcct
     3841 ccaggccggt gatgaacacg gcgtcgaact ccaggccctt cgccgagtgc accgtcatca
     3901 tctgcagcgc ttcctggccg tcgcccgcct ggtgctcgcc ggcctcgagc gcggcgtgcg
     3961 tcaggaacgc ggtaagcggg tcgaccgcct cgcccgactc gctgacgcgc tcctcctcgg
     4021 tgaaggcggc ggccgcgttc accagctctt ccaggttctc gacgcgatcg gcgccttcgc
     4081 gctcgttctt gtagtgctcg accaggccgc tcttcgcgat caccgcctcg atcatctcct
     4141 tcagcggcag cccctgcgtc tcgcttttca aatcctcgat caatttcctg aagacggcgg
     4201 ccttgccgtc ggccaccttg tagagcgaag ccccggcccg cttcgccgcc tcctgcagct
     4261 gctcgatggt gcgcgcgccg atgccgcgcg gcgggaagtt ggcgacgcgc aggaacgagt
     4321 tgtcgtcctc cggcgtggcg atcaggcgca ggtaggcgag cgcgtgcttg atctcggcgc
     4381 gctcgaagaa gcgcagtccg ccgtacaccc ggtaggggat gccttgcgag aacagcgcat
     4441 gctccaccac gcgcgactgg gcgttcgagc ggtagagcac cgcgatgtgc gaaaggcgcg
     4501 tgccgtcgcg cgcgagcgac tggacctcct cgacgatcca gcgcgcctcg tcgttgtccg
     4561 actcgccctc gaacacgcgc accggctcgc ccttgcccgc cgaggtccac aggttcttgc
     4621 ccaggcggtt gcggttgttg gcgatgagcg cgttggccgc gtcgaggatg ttgccgtgcg
     4681 agcggtagtt ctgctccagg cggatgacct tgcgcacctt gaactcgcgc tcgaactcgg
     4741 ccatgttgcc gacgttcgcg ccgcgaaaag tgtagatgct ctggtcgtcg tcgccgacgc
     4801 agaacaagta gttttctctc gaatagaaca atttcaacca gcgatactgc aagcggttgg
     4861 tgtcctggaa ctcgtccacc aggatgtagc ggaagcgccg cgcatagtgc tcgcgcagca
     4921 cttcgtttct cgtcagcaat tcatagcttc tgagaagcag ctcggggaaa tccaccaccc
     4981 cgtcgcgctg gcactgctcg tcgtaggcgg cgtagagctc ggcgaaccgc cgcgtgttgt
     5041 cgtcggccgg cggcacgtcg cgcgcgcgca cgccctcctc cttgcggtcg ttgatgaagt
     5101 actgcacctc gcgcggcggg aagcgctcct cgtcgacgtt catgcccttc agcaggcgct
     5161 tgatcgcggc gagctggtcc tgcgagtcga ggatctggaa cgccgccggc aacccggcct
     5221 cgcgcgcgtg cgcgcgcagc atgcggttgc acaggccgtg gaaggtgccg acccacatgc
     5281 cgcgcggatt gaccgtcatc atcgcgccga tgcgcgtcag catctcgcgc gcggccttgt
     5341 tggtgaaggt gaccgccagg atgcccgagg gcgcgacgcg cccgcctttc accagccagg
     5401 cgatgcgcgt ggtcaggacg cgcgtttttc cgctgcccgc gccggcgagg atgagcgcgt
     5461 gctcgtcggg cagggtgact gccgcgagct gctcgggatt tagatgctcg agatgaagcg
     5521 acaagccgcc attttacggg cgcttcattg caaaaccacc ggatcggcga tcccgaattg
     5581 cccgtcgagg cgccgggcga gcgcttgcgc ttcctcgcgc gacggcaagt ggcggatgcg
     5641 cacgacgtag ggcgcctgtc gcgcgtctgc gctcggcgcg atctccgcgg cgtagcccgc
     5701 gtcggcgagc tgctcctgca cctgggcgag cgcctcgcgc gtatcgccgg ccgcgagctg
     5761 cacgccgaag cgcccgccgc tgcgcagcgc gcccctgccg ggctcgatct cgacctcgcg
     5821 cgaccattgc tcgagctggc tcgcctcgac gaagccgacg ggctgcgccc tgcccttgtc
     5881 gcgctggtag aactggcgcg gccggtccag ggtgagcggc ggctcgccgg gcgcgccgac
     5941 ctggatcgcg ccctcgatca ggcacacgat ctggcgctcg ccgcgcccgc ggccccagaa
     6001 atcggtgccg cggatgccga tcgtcacctg ctccacgcgc accgtcacct cgcgcttgcg
     6061 cgccttggcg gcgaggtcgc tggtgaagcg gaaggcgcct tccagcacct gcagcgcggc
     6121 gcggaacagc tcgcgcgtcg gccgcatttc tgcaagccgc agggcgccgt tctcgccgag
     6181 cttcaccacg ctgccctcgg agagcttgac cagcacgcgc gcgtccttgc cgctgatgat
     6241 gcggtcgccc gcgcgcagct ccatccccgg gacgaggggc gtgcgcttgc cgtcgcgctc
     6301 gcgccacgcc ggcatctgca ccccgtccac gagcgccgcc gcctgcgtgt cacctccctg
     6361 ggcgagcgcc gcggcggagg tcagcgccag gagcacggca acgagcagcg ccttcatatt
     6421 gaatgagata atccgcgcct tcgcgaaaaa agtcacatgc aggaaaccta cgaccccgcc
     6481 gccgtcgaga aggaagcgca ggccttctgg gccgcgcgcg attccttccg ctccgtcgaa
     6541 gaccgggcca ggccgaaatt ctactgtctg tccatgttcc cctatccgtc ggggaagctg
     6601 cacatggggc acgtgcgcaa ctacaccatc ggcgacgtgc tgacgcgcta ccaccgcatg
     6661 cgcggctaca acgtgctgca gcccatgggc tgggacgcct tcggcctgcc ggccgagaac
     6721 gcggcgatgg cgaaccgcgt gccgccggcc agatggacct acgacaacat cgcctacatg
     6781 aagcggcagc tgcagggcct gggcttcgcg ctcgactgga gccgcgagct cgccacctgc
     6841 cggcccgact actaccgctg gaaccagtgg ctcttcacgc gcctgttcaa gaaggggctc
     6901 gcgtacaaga agaccggcgt ggtgaactgg gatccggtcg accagacggt gctcgcgaac
     6961 gagcaggtga tcgaggggcg cggctggcgc accggcgcgc tcgtcgagaa gcgcgagatc
     7021 ccgatgtact tcctgcgcat cacggcctac gccgaggagc tgctcgcggc gctggagaag
     7081 ctgcccggct ggcccgagca ggtgaagctg atgcagaaga actggatcgg caagtcggac
     7141 ggggtacgaa tcggttttcc ctacgagttg aacggcaaaa aggaaacgct ttgggttttc
     7201 accacgcgcg ccgacacgct gatgggcgcg accttcatgg cggtcgcggc ggagcacccg
     7261 atcgcggttt ttgcctccca aaaggacgcg aagctcaaag ccttcgtcga ggaatgccgc
     7321 aagggcgcgg tgatggaagc cgacctcgcg cagatcgaga agaagggcat gcccacgggc
     7381 ttgcatgcgc ttcacccgat taccaatgaa agaatcccga tctgggtggg caactacgtc
     7441 ctgatgagct atggcgaagg cgcggtgatg ggcgtgccgg cccacgacga gcgcgacttc
     7501 gcgttcgcgc tcaagtacaa gctgccgatc aagccggtga tccgccatcc gctcggcgac
     7561 accgtgccgg cgcc
//
LOCUS       DASRIE010000253        19352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_3373415, whole genome shotgun sequence.
ACCESSION   DASRIE010000253 DASRIE010000000
VERSION     DASRIE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373415"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..542
                     /locus_tag="VFB53_10985"
     CDS             171..542
                     /locus_tag="VFB53_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN87295.1"
                     /translation="MTSAALGSDVQMLETLNKGYVDAVQNSDVRWFDEHLTQDFLNSN
                     PDGTLVDRAGFLERIAKPVGVKGLAPQDVRIRLMGDFAIIHAKTVYRTPDGRAGAGRY
                     TDIWARRNGRWLCVAAHVTRG"
     gene            661..1227
                     /locus_tag="VFB53_10990"
     CDS             661..1227
                     /locus_tag="VFB53_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87296.1"
                     /translation="MLRKATLLLGSAAIFAFSLPASADNDSAMNGESRRIDRAAAGAN
                     GNDVATRIAADFPGTFGTPEETRGLIAELRGGDLQGSDKGALGYGEIYISLALAQELA
                     SASGMQPEDALNEVLGRRIEGQGWGQIAKDFDLKLGQVVSRARSGNERLAAAFTRPEK
                     ADRAERPAKPERPMRPERPERPERGGRN"
     gene            1240..1998
                     /locus_tag="VFB53_10995"
     CDS             1240..1998
                     /locus_tag="VFB53_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87297.1"
                     /translation="MRRIALSLALAAAAPWAAAADDMGSSLSIGIEQTTGDYGQAADT
                     TITYVPITAKLGNRPWQFGLTVPWIRVEGPGNVVRDVGRFRGAGGGTRTESGVGDVIA
                     FATRALGTMPDGTAVDATGKVKFGTASAGRGLGTGENDVHLQLDAYRSAGEFTPFGTV
                     GYKILGDPTGIELRNVFYISLGAARRLDTERSAGLMWYGQQKTTDGGDAQSEVTAFYT
                     RRFGGMWKAQFYGLIGMSDGSPDYGAGAMVIRAF"
     gene            2047..2328
                     /locus_tag="VFB53_11000"
     CDS             2047..2328
                     /locus_tag="VFB53_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87298.1"
                     /translation="MDEAEQQAKRQRLRELLAIPDRQRTDEQWDELNDLEIMFAAGNR
                     EGAPMPRQQGMPSQPGGYPRPGGGGGQNRNKQGRKFHHRPPKRNGPPDR"
     gene            2334..3290
                     /locus_tag="VFB53_11005"
     CDS             2334..3290
                     /locus_tag="VFB53_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN87299.1"
                     /translation="MARLLLAALAFCGLAQAQEPYPSRPIKLIVPFTPGTGMDILART
                     LGPGLTDKWKVPVVVENKPGASGNIGTEAVAKSPADGYTALVTASTLVMNRSLFRSIP
                     YDPVKDFAPVALLAVGSLALVTHPSVKADSVQELVALARANPDKVNYGSPGNGTPHHL
                     AMELFKQQTGTRMLHVPYKGTGPATQDLLGGQIQVMFLTVHVVLPLVQAGRLKMLAAG
                     GTERSIASPQVPSLAEASGIRNIDTDIWFGVYLPAGAPPEAVGKLNAELNALLKQPAI
                     TAALAKQGLQAMGGTPERLAQLTSADAERWARVVREAGIKPD"
     gene            3303..4511
                     /locus_tag="VFB53_11010"
     CDS             3303..4511
                     /locus_tag="VFB53_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HZN87300.1"
                     /translation="MADVLIVGGGIGGLTLAVDLRRRGIACRVFEAAPAIKPVGVGVN
                     LLPHSTKILAGFGLEPALARTGVETAESHFYNRYGQLIYREPLGRRAGLDWPQFSLHR
                     ADLQAALLERAGEIELGRQCAGFEQDAEGVTLRFADGGSARGAAAIGCDGIHSTIRRQ
                     LFPQDGAPRYSGVNMWRGVARWPAVLGGATMVRAGWLAKGKMVIYPIRNHADGTQLVN
                     WVFEIETPQYRPWDWNRPARIEDFCARVSDWKFGWLDVPAMLRASELVLEYPMVDKDP
                     LARWTQGRVTLLGDAAHPMVPRGSNGAGQAILDAHALADELARAAPPQALAAYEARRL
                     APTSKVVLENRSNPPDAILREVYERTGDRPFGRIEEVIAESELRAISERYKRVAGYDH
                     AALRQQEGTR"
     gene            4508..4906
                     /locus_tag="VFB53_11015"
     CDS             4508..4906
                     /locus_tag="VFB53_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN87301.1"
                     /translation="MSQFQPWGLLLGRLLLAYIFVLNGYGKIAGFAGTAKYMASKGMP
                     LIEPLLVGTILIELVGGLMLAVGWKARWAAWAFFLWLIPVTFIFHAYWSVPPEQVMAQ
                     TIQFQKNLAIMGGMLYVAFMGPGRFSLDKS"
     gene            4919..5266
                     /locus_tag="VFB53_11020"
     CDS             4919..5266
                     /locus_tag="VFB53_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN87302.1"
                     /translation="MAIIRHEPGPLLSLAVEHGDTVYLAGIVAKNLKNDVKGQTEEVL
                     AEIDRLLAKAGSHKSKILQATIWVNDIRHRAAVNEVYTKWVDPKNLPGRACIEAKLAD
                     PNALVEIMVTAAK"
     gene            complement(5288..5518)
                     /locus_tag="VFB53_11025"
     CDS             complement(5288..5518)
                     /locus_tag="VFB53_11025"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HZN87303.1"
                     /translation="MVKTTALALALALAAGPAFAQQKNCEELKAEIDAKITKNGVAKF
                     TTTIVAKDAPEDGKVVGTCEGGTKKIVYKRGG"
     gene            complement(5587..6393)
                     /locus_tag="VFB53_11030"
     CDS             complement(5587..6393)
                     /locus_tag="VFB53_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87304.1"
                     /translation="MRTLAAALLCFPLAALACGEVVTLKTRGEATLRYALAVPQSAAA
                     VLVLLPGGGGHADLDAKGCAQALKGNSLIRAQELFHKEGFATALVDAPSDHAGADGLG
                     GFRAQPAHAEDLGKVVADLRARLKLPVWLAGTSRGAISAANAAGRLSGAAAPDGVVIT
                     SPVTVGTPGGRLPWTAQTVFDAPLGRLAMPVLLVAHAADTCLRSPPEGIAGIAQRVKS
                     GRVQSVTVTGGPGTRQSGLAACEGRSPHGFTGQDAEVAEGIARFVRGGRY"
     gene            complement(6390..7196)
                     /locus_tag="VFB53_11035"
     CDS             complement(6390..7196)
                     /locus_tag="VFB53_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN87305.1"
                     /translation="MRLPRLGQGTWNMGDRPAQRAEEIAALRVGMELGMTVIDTAEMY
                     GNGRSESLVGEAIAGQRDRVFLVTKVLPSNAGRKGVSRACEASLKRLKTDHVDLYLLH
                     WMGSHPLEETLRGFEDLLAAGKIRSWGVSNLDIDEMQKLVSLPGGKACVTDQVLYNLS
                     RRGIEYDLLPWCAKNDISIMAYSPVEQGRILGNKALRQVAQRLGGTPAQVALAWTLRN
                     PNVVSIPKASTVAHVRENRKALELKLDAQALSQLDQAFPAPQGATPLEMI"
     gene            complement(7193..7600)
                     /locus_tag="VFB53_11040"
     CDS             complement(7193..7600)
                     /locus_tag="VFB53_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87306.1"
                     /translation="MESRRLLAATLLLAAGCAGLPAGTQPATVASFLGNPALCGHSGN
                     PVCEARVNHIEGKPVLSAAASIEVAPGRRTLGLFCKVGLSIMIGDAQSFQREVTVELA
                     PGGRYRVAAAMEPQPCTLALIDESTGKAVGTVR"
     gene            complement(7605..8429)
                     /gene="serB"
                     /locus_tag="VFB53_11045"
     CDS             complement(7605..8429)
                     /gene="serB"
                     /locus_tag="VFB53_11045"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZN87307.1"
                     /translation="MNLVLQPATEAQAAEAARLAGAQAVERAGEAAYRLRAAAANPAV
                     LAYCGGQRIDCAARDGAQRFADLKLLALDMDSTLITIECIDELGDLAGKKAEISEITA
                     LAMRGEIDYPESLYQRVALLRGLGVEALQRVYDERLRLSPGAEALVAACRRHGVRLLL
                     ISSGFSFFTDRLKQRLGLDFTVSNQLETHAGRLTGALVGDIVDAQTKAWRFLEAAQAL
                     GAAREQTVAIGDGANDLAMMAEAGTSVAFRAKPLVQAKASCALNYAGLDGVIPLFG"
     gene            complement(8426..11797)
                     /gene="mfd"
                     /locus_tag="VFB53_11050"
     CDS             complement(8426..11797)
                     /gene="mfd"
                     /locus_tag="VFB53_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZN87308.1"
                     /translation="MLREPVPLTPPVGSADALAVCRLAADGAPLVVISASAQDAQRLV
                     EEIAWFAPRLRACLLPDWETLPYDNFSPHQDLVSERLATLYRIQRGEFDVAVVPAQTA
                     LVRLCPPAYLAARTFFLEQGKRLDLEALKAQMATAGYRHVTQVVAPGEFCIRGGLIDL
                     FPTGSRLPYRLDLDDELIDAIRSFDVDTQRTLYAVKEVRMLPAREFPLDEAGRAKFRS
                     RFREVFEGDPSKKGLYRDVSNGIAAAGIEYYLPLFFDAVATLFDYLPKTAGLARHGDV
                     PAAVDAFWRDAASRYRLLGGERERPLLPPAQVFVPAEEFHAAAGGLRRVALEAGPASA
                     PLAALAVDRRAQDPLAAFKAFLAQSAQRVLVAAESPGRRETMLGYFAEYGLQPAPCAD
                     FAAWLSAGERFAITVAPLAGGFSLPGEGWCVVTEAELYAGTVRRRARDAKKQSSVEGM
                     LRDLSELRVGDPVVHAQHGIGRYQGLIGMDLGEGPTEFLHLQYEGGDKLYVPVSQLAV
                     ISRYSGAEAEAAPLHKLGSGQWDKAKARAARQVRDTAAELLALYARRAAREGHAFRVQ
                     AHDLDAFADGFGFEETADQAAAIQAVAADMAAGKPMDRLICGDVGFGKTEVALRAAFI
                     AVADRKQVAILCPTTLLVEQHFQTFSDRFADWPVRIAELSRFRSAKEAAATLNALAAG
                     EVDILIGTHKLLSKDVKFQRLGLAIVDEEHRFGVRQKEALKALRAEVDVLTLTATPIP
                     RTLVLSLEGLREFSVIATAPQRRLAIKTFAAPFSEGLIREAALRELKRGGQVYFLHNE
                     VDTIASMRERLARLLPEARIAVAHGQMRERELERVMRDFYAQRSSLLLCTTIIETGID
                     VPSANTIVIHRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPPEEALSANAKKRLEAIQ
                     AMEELGAGFYLAMHDLEIRGAGEVLGESQSGEIQEIGFNLYAQMLERAVKALKSGRHV
                     DLAQPLDIATEINLHTPALLPEGYCSDVHERLTLYKRMANCESRQQLDELREELVDRF
                     GELPEAARALLECHALRIAAKPVGVARVDATHESVQLQFDKHPPIDGAKIIALVQRRK
                     DLRLTGPEKLRLAAKLPAWQERAQAVRDLFMQLAA"
     gene            11841..12533
                     /gene="ispD"
                     /locus_tag="VFB53_11055"
     CDS             11841..12533
                     /gene="ispD"
                     /locus_tag="VFB53_11055"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZN87309.1"
                     /translation="MTWFALIPAAGSGSRIAGALPKQYLDLNGRPMLWHAVHAVCRAP
                     VETVFVVLTPGDAAFPRYDWSAFAGRLEPLYCGGATRRDSVYNGLVAAMAAVDADDWM
                     LVHDAARPCLPMADLARLVAECRGDEIGGILALPAADTVKRAAKDESGATRVAGTEDR
                     DLLWLAQTPQMFRAGLLAEALRQARATVTDEASAVEALGLRPRLVAGSRENLKVTYPE
                     DVAMAKAILARR"
     gene            12535..13008
                     /gene="ispF"
                     /locus_tag="VFB53_11060"
     CDS             12535..13008
                     /gene="ispF"
                     /locus_tag="VFB53_11060"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZN87310.1"
                     /translation="MRIGQGYDVHALVPGRKLVIGGVRIPFHLGLEGHSDADVLLHAI
                     CDALLGAAGLGDIGRHYPDTDPQYSEIDSRELLRGVAKKLAAQNCRVVNVDATVLAQA
                     PRMAPHFARMTGNIAADLGVAPAAVNLKATTTEGLGAIGRGEGIAALAVALIEEA"
     gene            13010..13627
                     /locus_tag="VFB53_11065"
     CDS             13010..13627
                     /locus_tag="VFB53_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZN87311.1"
                     /translation="MYSPPYNQVEDRAELLDFMRANSFALLVTATGGAPLASHLPVTL
                     ADGAGGIVIHSHMARNNPQWQEFFEDEVLVVFAGPHAYVSPRWYEARERVPTWNYAAV
                     HAYGTVQVTEDRAVKHAAQRALVAELDPQWLPQFDALRPAYVQNMLEGIVTFDIAVTR
                     LETRWKLSQNRGRREQELIAAELERSADSSARALAALTRKHYVAE"
     gene            complement(13577..13813)
                     /locus_tag="VFB53_11070"
     CDS             complement(13577..13813)
                     /locus_tag="VFB53_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87312.1"
                     /translation="MKHVRSNPGLAALYARLDEIRMSPADRLAARSALAQADALADFL
                     LGAMRLVRRLAGARPRGLLLRDVMLAGQRRQRAR"
     gene            complement(13982..15262)
                     /locus_tag="VFB53_11075"
     CDS             complement(13982..15262)
                     /locus_tag="VFB53_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN87313.1"
                     /translation="MTLFWRSFLLIALLLVVSVLASFQISRAYEREPRERELAQQAVS
                     VVNLTRAALVSADPVLRRDLLIELNEREGIRIYPATNEEALRPLRPAGRLDRVAASVR
                     AQLGAATRFAAARDGMRGFWVSFFIDEDEFWVLLPRERFEPEFGLGWLGWGAALLALA
                     LLGAWLIASRISRPLAGLAAAARRLGHGETPEPLPVAGELELRTVQAEFNRMAGDLQS
                     LERERAMVLAGISHDLRTPLSRLRLGVEMSGADAATAEGMNADIEEMDRVLGQFLDFA
                     RGDNEARGAASLGALLAEIGEHYARLGRKVEVAAPDLAPFAFAPKALRRAVGNLIENA
                     LRYAGEPVAVQARRAGGEVLIEVLDRGPGIPAGEAERLKRPFARLDPARSGQGGAGLG
                     LAIVERVARTHGGRFELDPREGGGLAARLYLPAS"
     gene            complement(15259..15984)
                     /gene="ompR"
                     /locus_tag="VFB53_11080"
     CDS             complement(15259..15984)
                     /gene="ompR"
                     /locus_tag="VFB53_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HZN87314.1"
                     /translation="MAELKTRILVVDDDQRLRDLLTRYLGEQKFEVRAVRDAPEMDKY
                     LSRERYDLLVLDLMLPGEDGLAICRRLRAAGGSPAIIMLTAKGDEVDRIVGLEMGADD
                     YLPKPFNPRELVARINAVLRRRPRPGAPGAPSSEDGRHRFGAFEFDPATRELKKDGKP
                     VPLTTGEFSVLKVLVERPRQPLSRDQLMELARGREYEVFDRSIDVQISRLRKLVEADP
                     AHPRYIQTVWGHGYVFVPDGTPR"
     gene            16042..16293
                     /locus_tag="VFB53_11085"
     CDS             16042..16293
                     /locus_tag="VFB53_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87315.1"
                     /translation="MKTLLALLLACAVCLPALGQERAARDRNLQKQGLQPHERMREER
                     RELRRERPDRAEGRHFSREERQRLRQDLIDANRSMKGRR"
     gene            complement(16294..17700)
                     /locus_tag="VFB53_11090"
     CDS             complement(16294..17700)
                     /locus_tag="VFB53_11090"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN87316.1"
                     /translation="MADSVAVPLWLALAGALLALWALYEHALMPALRWLVTHPANQVI
                     DEVGKRLRIGIRPFQRTRRQALIHRLLTDPKVQQAAEQFAAQNKVPVLVALELVERYA
                     RETVPAFNAYLYFRIGYWFGKRVAQLLYRVRLGYVDVENLQRVEPDSTVVFVMNHRSN
                     MDYVLAGYLAADQTALSYAVGEWARIWPLSALIRSMGAYFVRRNSKDELYRRVLERYI
                     VMATEAGVPQAVFPEGGLTRDGRMREPRLGAIDYMMRGFRADGERDLVFVPLGINYDR
                     VLEDRTLLRSIDPDAARRGRLAALWTTLRFAAHNFGLMLRSEWHRFGYACVNFGAPVS
                     MREYCRQHGLDFHRLAGEERKREMAALGRHLMDAVGRIVPVLPVPLIATVFVAHGDKA
                     ISEMELKSEVSRLVEQLQARGAHVYVPRKDFDYAVNVGLRMLRLRRLVGEREGLYYAQ
                     AEELPLLRYYANSIAHLR"
     gene            complement(17726..18601)
                     /locus_tag="VFB53_11095"
     CDS             complement(17726..18601)
                     /locus_tag="VFB53_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN87317.1"
                     /translation="MLQADSARNRLTGIGLISLTYVLFTLLDGSAKWLVQTVPVLVVV
                     WLRFLSQVVIASALLFPIRGRALVRTSHLRWHLVRGLMFVAMTGMNFWALQFLQLTVT
                     ASIFFMVPLLVALGAAPLLGEKLDAKRWAAILVGFAGVLVIVRPGSESFHPAMLLSVA
                     NAVLYAAFNMMTRKLAAYDPPETIQFLPAVVASVVLAPFALAAWQSPPGWFEWLLLCL
                     MGVFGGTGHYLLAMAHRYAPASTLAPFLYQQILYMALFGYLVFGSVPDPAVWIGAAIV
                     IASGLYLFSRERRRR"
     gene            complement(18601..>19352)
                     /locus_tag="VFB53_11100"
     CDS             complement(18601..>19352)
                     /locus_tag="VFB53_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZN87318.1"
                     /translation="VRPSKLLPGVIGPLRPELGAGIDWVIVRENSEGEYAGVGGRAHR
                     GLPIEVGMDVAVFTRAGVERIARYACDLARSRPRKLLTVVTKSNAQRHGMVLWDEIFY
                     EVAKKYPEVKTDRILVDAMTTRMVLKPGSLDTIVASNLHADILSDLAAALSGSLGIAP
                     TANLNPERKFPSMFEPIHGSAFDITGKGVANPIATFWTAALMLEHLGEPKAAARLMAA
                     IERTTEEQVFTPDLGGAHDTRAVTSAVKRAL"
BASE COUNT         2953 a         6781 c         6895 g         2723 t
ORIGIN      
        1 gtgcgcgcgc tcaccgagat ttggaccggc gacagcgacc cgaagaccga gatcgcggca
       61 ggaagcgtgc gggtgagcgg cggcgggcga tccggcacgg cgatctggcg gtggctcggc
      121 cgcagcatgt tcgcgccgag ccggcggaac ctctggcaag gggaagcgtc atgacaagcg
      181 cagcactggg aagcgacgtg cagatgctgg agacgctcaa caagggctac gtggacgcgg
      241 tgcagaattc cgacgtgcgc tggttcgacg agcacctgac gcaggatttc ctcaactcca
      301 acccggacgg cacgctggtg gatcgcgccg gcttcctcga gcgcatcgcc aagcccgtcg
      361 gcgtgaaggg cctggcgccg caggacgtga ggatccgcct gatgggcgac ttcgccatca
      421 tccacgcgaa gaccgtctat cgcacgcccg acggccgcgc cggcgccggg cgctacaccg
      481 acatctgggc gcggcgcaac ggccgctggc tctgcgtcgc cgcgcacgtg acgcgcggat
      541 aatcagccgt cgccatttgg cgacgcgatt tcgtcgccga acaccgacag tgcacacccg
      601 cactgccggc cctgccttct ctaaaattgc atcagcaatt cttggagaag gaggcccgtc
      661 atgctccgaa aagccacgct cctgctaggt agtgcggcga tcttcgcgtt ttccctcccc
      721 gcatccgccg acaacgactc ggcgatgaac ggcgaatcgc gccgcatcga ccgcgccgcc
      781 gccggcgcga acggcaacga cgtcgcgacg cgcatcgccg cggatttccc cggcaccttc
      841 ggcaccccgg aggaaacgcg cgggctcatc gccgagctgc gcggcggcga tctgcagggc
      901 agcgacaagg gcgcgctcgg ctacggcgag atctacattt cgctcgccct ggcgcaggag
      961 ctggcgagcg cttccggcat gcagcccgag gacgcgctga acgaggtcct cggccggcgc
     1021 atcgagggcc agggctgggg ccagatcgcc aaggacttcg acctcaagct tggccaggtg
     1081 gtgtcgcgcg cccgctcggg caacgagcgc ctggcggcgg ctttcacgcg gccggagaaa
     1141 gcggatcgcg ccgagcgacc ggcgaagccc gagcgcccga tgcggcccga gcgtccggag
     1201 cgccccgagc gcggcggccg gaactagggg ccgcgcaaga tgcgcaggat cgcgctttcc
     1261 ctcgcgctcg ccgccgccgc gccctgggcg gcggcggccg acgacatggg cagcagcctg
     1321 tcgatcggca tcgagcagac cacgggcgac tacggccagg ccgccgacac caccatcacc
     1381 tacgtgccga tcacggcgaa gctgggcaac cggccctggc agttcggcct caccgtgccc
     1441 tggatccgcg tcgaaggccc gggcaacgtg gtgcgcgacg tcgggcgctt ccgcggcgcc
     1501 ggcggcggca cgcgcaccga gtccggggtg ggcgacgtga tcgccttcgc cacccgcgcc
     1561 ctcggcacga tgcccgatgg caccgccgtc gacgccaccg gcaaggtcaa attcggcacg
     1621 gcgagcgccg gccgcggcct gggcacgggc gagaacgacg tgcacctgca gctggacgcc
     1681 taccgcagcg cgggcgagtt cacgccgttc ggcaccgtgg gctacaagat cctcggcgat
     1741 cccacgggca tcgagctgcg caatgtgttc tacatctcgc tcggcgcggc gcggcgcctc
     1801 gacacggaac gcagcgccgg gctgatgtgg tacgggcagc agaagaccac cgacggcggc
     1861 gacgcgcaaa gcgaggtcac cgcgttctac acgcgccgct tcggcggcat gtggaaggcc
     1921 cagttctacg gcctgatcgg catgtccgac ggcagcccgg actacggcgc gggggcgatg
     1981 gtcatccgcg cgttctgaac gctcggcggg cgccctcgcc cgggcttacg cagttacaat
     2041 gcccggatgg acgaagccga acagcaagcc aagcgccagc gcctgcggga actgctcgcg
     2101 attcccgatc gccagcgcac ggacgagcag tgggatgagc tgaacgacct ggagatcatg
     2161 ttcgccgcgg gcaaccgcga aggcgcgccg atgccgcgcc agcagggcat gccctcgcag
     2221 ccgggcggct atccccgccc cggcggcggc ggcggacaga atcgcaacaa gcaggggcgc
     2281 aagttccacc accggccgcc caagcgcaac gggcccccgg accggtgagc gacatggcga
     2341 gactgctgct cgcggcgctg gcgttctgcg gactcgccca ggcgcaggaa ccctatccgt
     2401 ccaggccgat caagctgatc gtgccgttca cgccgggcac cggcatggac atcctcgcgc
     2461 gcaccctcgg gccgggtctc acggacaagt ggaaggtgcc ggtggtggtg gaaaacaagc
     2521 ccggcgcgag cggcaacatc ggcaccgagg cggtggcgaa gtcgccagcc gacggctata
     2581 ccgcgctggt caccgccagc acgctggtga tgaaccgcag cctgttcagg agcattccct
     2641 acgacccggt gaaggatttc gcgccggtgg cgctgctcgc cgtgggcagc ctggcgctgg
     2701 tgacgcaccc ttcggtgaag gcggacagcg tgcaggagct ggtggcgctg gcgcgcgcga
     2761 atccggacaa ggtgaactac ggctcgccgg gcaacggcac gccgcaccac ctcgccatgg
     2821 agctgttcaa gcagcagacc gggacgcgca tgctgcacgt cccctacaag ggcaccgggc
     2881 cggcgacgca ggatctgctc ggcggccaga tccaggtgat gttcctgacc gtccatgtgg
     2941 tgctgccgct ggtgcaggcg ggcaggctga agatgctcgc cgccggaggc accgagcgct
     3001 ccatcgcttc gccgcaggtg ccttcgctcg ccgaggcctc gggcatccgc aatatcgaca
     3061 cggacatctg gttcggcgtc tacctgccgg cgggcgcgcc gccggaggcg gtggggaagc
     3121 tgaacgccga gctgaacgcg ctgctcaagc agcccgccat cactgcagcc ctggccaagc
     3181 agggcctgca ggcgatgggc gggacgccgg agcggctggc gcaactgacc agcgccgacg
     3241 ccgagcgctg ggcgcgggtg gtgcgggagg cgggcatcaa gcccgactga gccggagatt
     3301 cgttggctga cgtcctcatc gtcggcggcg gcatcggcgg cctgaccctc gccgtggatc
     3361 tgcgccgtcg cggcatcgca tgccgcgtgt tcgaggcggc gccggcgatc aagccggtgg
     3421 gcgtcggcgt gaacctgctg ccgcactcga cgaagatcct cgccggcttc ggcctggagc
     3481 ccgcccttgc gcgcacgggg gtggaaaccg ccgagtcgca tttctacaac cgctacggcc
     3541 agctgatcta ccgggagccg ctcggccggc gcgcgggcct cgactggccg cagttctccc
     3601 tgcatcgcgc cgacctgcag gcggcgttgc tcgagcgcgc cggcgagatc gagctgggca
     3661 ggcagtgcgc gggcttcgag caggacgcgg agggcgtgac cctgcgcttt gccgacggcg
     3721 gcagcgcgcg cggcgcggct gcgatcggct gcgacggcat ccactcgacg atccgccgcc
     3781 agctgttccc gcaggacggc gcgccgcgct actcgggcgt gaacatgtgg cgcggcgtcg
     3841 cgcgctggcc ggcggtcctc ggcggcgcga cgatggtgcg cgccggctgg ctcgccaagg
     3901 gcaagatggt gatctacccc atccgcaacc acgccgacgg cacccagctc gtcaactggg
     3961 tgttcgagat cgagacgccg caataccgcc catgggactg gaaccggccg gcgcgcatcg
     4021 aggatttctg cgcgcgggtc tcggactgga agttcggctg gctcgacgtt ccggcgatgc
     4081 tgcgcgcctc cgagctggtg ctggaatacc cgatggtgga caaggacccg ctcgcgcgct
     4141 ggacgcaggg gcgcgtcacg ctgctcggcg atgcggcgca cccgatggtc ccgaggggct
     4201 ccaacggggc ggggcaggcg atactcgacg cgcacgcgct ggccgacgaa ttggcgcgtg
     4261 ctgcgccgcc gcaggcgctc gccgcctacg aggcccggcg cctcgcgccg accagcaagg
     4321 tggtgctgga gaaccggagc aacccgcccg acgccatcct gcgcgaggtc tacgagcgca
     4381 ccggcgaccg gcccttcggc cggatcgagg aggtgatcgc ggagtccgaa ttgcgcgcca
     4441 tctcggagcg ctacaagcgg gtcgcggggt atgatcacgc agctcttcga caacaggagg
     4501 gaacaagatg agccaattcc aaccgtgggg actattgctt ggacgcctgc tgctggcgta
     4561 catcttcgtg ctcaacggct acggcaagat cgccggcttc gccggcaccg ccaagtacat
     4621 ggcatcgaag ggcatgccgc tgatcgagcc gctgctggtg ggcaccatcc tcatcgagct
     4681 ggtcggcggc ctgatgctgg cggtgggctg gaaggcccgc tgggcggcct gggcgttctt
     4741 cctgtggctg attccggtca cattcatctt ccatgcctac tggagcgtgc cgccggagca
     4801 ggtgatggcg cagaccatcc agttccagaa gaacctcgcc atcatgggcg gcatgctcta
     4861 tgtcgcgttc atggggccgg gcaggttcag cctcgacaag tcctaggaga agccaggcat
     4921 ggccatcatc cggcacgaac ccggtccgct gctgtcgctc gcggtggaac acggcgatac
     4981 cgtgtacctg gcgggaatcg tcgcgaagaa cctgaagaac gacgtcaagg gccagaccga
     5041 agaggtgctg gcggagatcg accggctgct ggccaaggcc gggtcgcaca agtcgaagat
     5101 cctgcaggcc accatctggg tcaacgacat ccgccaccgc gcggcggtga acgaggtcta
     5161 tacgaagtgg gtggacccga agaacctgcc gggccgcgcc tgcatcgagg cgaagctcgc
     5221 cgacccgaac gcgctggtcg agatcatggt gaccgcggca aagtagccgc ggccaccggg
     5281 cgggcggcta gcccccgcgc ttgtagacga tcttcttggt gccgccttcg caggtgccga
     5341 cgaccttgcc gtcctcgggc gcgtccttgg cgacgatggt ggtggtgaac ttggccacgc
     5401 cgttcttggt gatcttggcg tcgatctcgg ccttcaattc ctcgcagttc ttctgctggg
     5461 cgaaggcggg gccggcagcc agggcgaggg caagggcgag tgcggtggtt ttgaccatct
     5521 gttttctccc gggttagtcg aagtgcggcg ctattgtgcg cccgcgcccc tgggcgggga
     5581 agggggtcag tagcgcccgc cgcgcacgaa gcgggcgatg ccctccgcga cctcggcatc
     5641 ctggccggtg aagccgtgcg gcgagcggcc ctcgcaggcc gcgagcccgc tttggcgggt
     5701 gccgggcccg ccggtgaccg tgacggattg cacgcggccg ctcttcacgc gctgcgcgat
     5761 gccggcgatg ccctcgggcg gggagcgcag gcaggtatcg gcggcgtgcg ccacgagcag
     5821 caccggcatg gccagcctcc cgagtggcgc atcgaacacc gtctgcgccg tccagggcag
     5881 ccgcccgccc ggcgtcccga cggtcaccgg cgaggtgatc accacgccgt cgggcgcggc
     5941 ggcgccggac aggcgccctg ccgcgttcgc cgccgatatc gcgccgcggc tggtgcccgc
     6001 cagccacacc ggcagcttca gccttgcgcg caggtcggcg accactttgc ccaggtcctc
     6061 ggcgtgcgcc ggctgcgcgc gaaacccgcc caggccgtcc gcgccggcgt ggtccgaagg
     6121 cgcatccacc agcgcggtgg cgaagccttc cttgtggaag agctcctgcg cccggatcag
     6181 cgaattgccc ttcaacgcct gcgcgcagcc tttggcatcc aggtcggcat gcccgccgcc
     6241 gccgggcaga aggaccagga ccgcggcagc gctttgcggc accgcgagcg cgtagcgcag
     6301 ggttgcttca ccgcgggtct tcagggtgac gacttcgccg caggcgagcg cggcgagggg
     6361 aaagcacagc agggcggcgg cgagagttct caaatcatct ccagtggggt tgcgccctgc
     6421 ggcgcgggga aggcctggtc cagttgcgac agcgcctgcg cgtccagctt gagctcgagc
     6481 gccttgcggt tctcgcgcac gtgcgcgacc gttgacgcct tcgggatgga cacgacgttc
     6541 ggattgcgca gcgtccaggc gagcgcgacc tgggcggggg ttccccccag gcgctgcgcc
     6601 acctgccgca atgccttgtt gcccaggatc cggccttgct ccaccgggga atacgccatg
     6661 atcgaaatat cgttcttcgc gcaccagggc agcagatcgt actcgatgcc gcggcgcgaa
     6721 aggttgtaga gcacctggtc ggtgacgcag gccttgccgc cgggcaggga aaccagcttc
     6781 tgcatctcgt cgatgtccag gttgctcacg ccccacgacc ggatcttgcc tgcggcgagc
     6841 aggtcctcga agccgcgcag cgtctcctcg agcgggtgcg agcccatcca gtgcagcagg
     6901 tacaggtcca cgtgatcggt cttcagccgc ttcaggctcg cctcgcaggc gcggctgacg
     6961 cccttgcggc cggcgttcga gggcagcacc ttggtgacga ggaacacccg gtcgcgctgg
     7021 ccggcaatgg cttcgcccac cagcgactcc gagcgtccgt tgccgtacat ctccgcggtg
     7081 tcgatcacgg tcatgcccag ctccatgccc acgcgcagcg cggcgatctc ctcggcgcgc
     7141 tgcgctggcc ggtcgcccat gttccaggtg ccctggccga ggcgcggcag cctcatcgca
     7201 ccgtgcccac cgccttgccc gtggactcgt cgatcagcgc cagggtgcag ggttgcggct
     7261 ccatcgcggc cgccacgcgg tagcgcccgc cgggcgcgag ctccacggtc acttcgcgct
     7321 ggaaactctg cgcgtcgccg atcatgatgc tcagcccgac cttgcagaac agtcccagcg
     7381 tgcgccggcc cggcgccacc tcgatgctcg ccgcggcgga aagcacgggt ttgccttcga
     7441 tgtggttcac gcgcgcctcg cacaccgggt tcccgctgtg gccgcagagg gcgggattgc
     7501 cgaggaacga ggcgacggtc gccggctgcg tgcccgcggg caggcccgcg catccggcgg
     7561 cgaggagcag cgttgcggcg aggagtcggc ggctctccat gcactcaccc gaacaggggg
     7621 atcaccccgt ccagtcccgc gtagtttaaa gcgcaggagg ccttcgcctg caccaggggc
     7681 ttcgcccgga aggccaccga ggtcccggcc tcggccatca tcgcgaggtc gttcgccccg
     7741 tcgccgatgg ccacggtctg ctcgcgcgcg gcgccaagcg cctgcgccgc ctcgaggaag
     7801 cgccacgcct tggtctgagc gtcgacgatg tcgccgacca gtgctccggt gagcctgccc
     7861 gcgtgcgtct cgagctggtt ggaaaccgtg aagtcgaggc ccagccgctg cttcagccgg
     7921 tcggtgaaga agctgaagcc gctggagatc aggaggagcc gcacgccgtg ccgccggcag
     7981 gccgcgacca gggcctccgc cccgggcgag agccgcaggc gctcgtcgta cacccgctgc
     8041 agcgcctcca cgccgagccc ccggagcagc gcgacgcgct ggtacaggct ctccgggtag
     8101 tcgatctcac cgcgcatcgc cagcgcggtg atctcggata tctccgcttt cttccccgcc
     8161 aggtcgccca gctcgtcgat gcactcgatg gtgatgagcg tggaatccat gtccagggcg
     8221 agcagcttca ggtcggcaaa gcgctgcgcg ccgtcgcgcg cggcgcagtc gatgcgctgc
     8281 ccgccgcagt aggcgaggac ggcggggttg gccgcggctg cgcgcaggcg gtaggcggct
     8341 tcgccagccc gctcgacagc ctgcgcgccg gcgaggcggg cggcctctgc ggcctgcgct
     8401 tcggtcgcgg gctgcaggac gaggttcacg cggcgagctg catgaacagg tcgcgcaccg
     8461 cctgcgcacg ctcctgccag gcgggcagct tcgccgccag gcgcagcttt tccgggccgg
     8521 tgagccgcag gtccttcctg cgctgcacca gggcgatgat cttcgcaccg tcgatgggcg
     8581 ggtgcttgtc gaactgcaat tgcaccgatt cgtgcgtggc gtcgacgcgc gccaccccga
     8641 cgggtttggc ggcgatgcgc agggcgtggc actcgagcag ggcgcgcgcc gcctcgggca
     8701 gctcgccgaa gcggtccacc agctcctcgc gcagctcatc cagctgctgg cgcgactcgc
     8761 agttggccat gcgcttgtac agcgtgagcc gctcgtgcac gtcgctgcag tagccctcgg
     8821 gcaacagcgc cggggtgtgc aggttgatct cggtggcgat gtccagcggc tgcgccaggt
     8881 cgacgtgccg tcccgacttg agggccttga cggcgcgctc gagcatctgc gcgtacaggt
     8941 tgaagccgat ctcctggatc tcccccgatt gcgactcgcc cagcacctcc cccgcgccgc
     9001 ggatctccag gtcgtgcatg gcgaggtaga agcccgcgcc cagctcctcc atcgcctgga
     9061 tcgcctcgag gcgcttcttg gcgttggccg agagcgcctc ctcgggcggg gtgagcaggt
     9121 aggcgtaggc ctggtggtgc gagcgcccga cgcgcccgcg cagctggtgc agctgcgcca
     9181 ggccgaactt gtcggcgcgg tggatcacga tggtgttggc gctgggcacg tcgatgcccg
     9241 tctcgatgat ggtggtgcac aggagcaggc ttgagcgctg cgcgtagaag tcgcgcatca
     9301 cgcgctccag ctcgcgctcg cgcatctggc cgtgcgccac cgcgatccgc gcctcgggca
     9361 ggaggcgcgc caggcgctcg cgcatcgatg cgatcgtgtc cacctcgttg tgcaggaagt
     9421 acacctggcc gccgcgcttg agctcccgca gcgccgcttc gcggatcagg ccctcggaga
     9481 acggcgcggc gaaagtcttg atcgccaggc gccgctgcgg cgcggtggcg atcaccgaga
     9541 actcgcgcag gccctcgagc gacagcacca gcgtccgcgg aatcggcgtg gcggtgaggg
     9601 tgagcacgtc cacctcggcg cgcagcgcct tgagcgcctc cttctgccgc accccgaagc
     9661 ggtgctcctc gtcgacgatg gcgaggccca ggcgctggaa cttcacgtcc ttcgacagca
     9721 gcttgtgcgt gccgatgagg atgtccacct cgccagcggc aagcgcattg agcgttgccg
     9781 cggcctcctt ggcggagcgg aagcgcgaca gctcggcgat cctcaccggc cagtcggcga
     9841 aacggtccga gaaggtctgg aagtgctgct ccaccagcag cgtcgtaggg cagaggatcg
     9901 ccacctgctt gcggtcggcg acggcgatga aagccgcgcg cagcgccacc tcagtcttgc
     9961 cgaaaccgac atcgccgcag atcaggcggt ccatcggctt gccggccgcc atgtccgcgg
    10021 ccaccgcctg gatggcggcc gcctggtcgg cggtctcctc gaagccgaag ccgtcggcga
    10081 aagcgtccag gtcgtgcgcc tgcacgcgga aagcatggcc ctcgcgcgcc gcgcgccgtg
    10141 catacagcgc gaggagctcg gcggccgtgt cgcgcacctg cctggcggcg cgcgccttcg
    10201 ccttgtccca ctggccgctg cccagcttgt gcagcggagc ggcctcggcc tcggcgcccg
    10261 agtagcgcga gatcaccgcc agctgcgaca ccggcacgta cagcttgtcg ccgccttcgt
    10321 attgcaggtg caggaattcg gtcggacctt cgcccaggtc cattccgatc aggccctggt
    10381 agcgcccgat gccgtgctgc gcgtgcacca ccgggtcgcc gacgcgcaat tccgacaggt
    10441 cgcgcagcat gccttccacc gagctttgct tcttcgcgtc gcgggcgcgc cgccgcacgg
    10501 tgccggcata cagctcggct tccgtgacca cgcaccagcc ttcgcccggc agcgagaagc
    10561 cgccggcgag cggcgccacg gtgatggcga agcgctcccc ggcgctcagc caggcggcga
    10621 agtcggcgca gggcgcgggc tgcagaccgt actcggcgaa atatcccagc atggtctcgc
    10681 gccgccccgg cgattcggcg gccaccagga cgcgctgcgc ggactgcgcg aggaaggcct
    10741 tgaacgcagc gagcggatcc tgggcgcgcc ggtcgaccgc cagcgcggcc agcggcgcgc
    10801 tggccgggcc cgcttccagc gccacgcgcc gcaggccgcc cgcggcggcg tggaactcct
    10861 cggccggcac gaacacctgc gccggcggca gcaggggccg ctcgcgctcg ccgccgagca
    10921 gccggtagcg cgaagcggcg tcgcgccaga aggcatcgac cgccgccggc acgtcgccgt
    10981 ggcgcgccag gccggccgtc ttcggcaggt agtcgaacag cgtcgccacc gcgtcgaaga
    11041 acaggggcag gtagtactcg atgccggccg cggcgatgcc gttggagacg tcgcggtaca
    11101 ggcccttctt ggacggatcg ccctcgaaaa cctcccggaa gcggctgcgg aacttcgcgc
    11161 gtcccgcctc gtcgagcgga aactcgcgcg ccggcagcat gcgcacttcc ttcaccgcat
    11221 acagcgtgcg ctgggtgtcc acgtcgaagc tgcggatggc gtcgatcagc tcgtcgtcga
    11281 gatccagccg gtagggcagc cggctgccgg tggggaaaag atcgatcagc ccgccgcgga
    11341 tgcagaactc gcccggcgcc accacctgcg tgacgtggcg gtagcccgcc gtcgccatct
    11401 gcgccttgag agcctcgagg tccaggcgct tgccttgctc gaggaagaaa gtcctcgcgg
    11461 cgaggtaggc gggcgggcac aggcgcacca gcgcggtctg cgccggcacc acggcgacgt
    11521 cgaactcgcc gcgctggatg cggtagagcg tcgcgaggcg ctcggacacc aggtcctgat
    11581 gcggcgagaa attgtcgtaa ggcagggtct cccagtccgg cagcaggcat gcgcgcagcc
    11641 ggggcgcgaa ccaggcgatc tcttcgacca ggcgctgcgc gtcctgcgcg ctggcgctga
    11701 tcaccaccag cggggcgccg tcggcggcga ggcggcacac ggcgagcgca tccgccgagc
    11761 cgacgggcgg ggtcaaggga accggttcgc ggagcatgcc gaaacgaatt atacttgctt
    11821 gaattcatgg agtttttgcg ttgacctggt ttgcgctgat ccccgctgcc ggcagcggca
    11881 gccgcatcgc cggcgcgctg cccaagcagt acctcgacct caacggccgg ccgatgctgt
    11941 ggcacgcggt gcacgcggtg tgccgggcgc cggtggaaac ggtattcgtg gtactcaccc
    12001 cgggcgacgc ggcgtttccg cgctacgact ggagcgcttt cgccggcagg ctggagccgc
    12061 tgtactgcgg cggcgcgacg cgccgcgatt cggtctacaa cggcctggtg gcggcgatgg
    12121 cggcggtgga tgcggacgac tggatgctgg tgcacgatgc ggcgcgcccc tgcctgccga
    12181 tggcggacct cgcgcgcctg gtcgccgagt gccgcggcga cgagatcggc ggcatcctcg
    12241 cgctgccggc ggccgacacc gtgaagcgcg ccgccaagga cgagtcgggc gcgacgcgcg
    12301 tcgccggcac cgaggatcgc gacctgctgt ggctggcgca gacgccgcag atgttccgcg
    12361 ccgggctgct cgccgaggcg ctgcgccagg cgcgcgccac ggtgaccgac gaggcgagcg
    12421 cggtcgaggc gctgggcctc aggccgcggc tggtcgcggg cagccgcgag aacctgaagg
    12481 tcacgtaccc ggaagacgtg gcgatggcga aagcgatact ggcgaggcga tgacatgaga
    12541 atcggacaag gctacgacgt gcacgcgctg gtccccggcc gcaagctggt gatcggcggg
    12601 gtgcggatcc cgtttcacct cggcctggag gggcactcgg acgcggacgt gctgctgcac
    12661 gcgatctgcg acgcgctgct cggcgccgcg gggctgggcg acatcggccg gcactacccc
    12721 gacaccgacc cgcagtattc cgagatcgac agccgcgagc tgctgcgcgg cgtggcgaag
    12781 aagctcgccg cgcagaactg ccgcgtggtg aacgtggatg ccaccgtgct ggcccaggcg
    12841 ccgcgcatgg cgccgcattt cgcgcgcatg acgggaaaca tcgccgccga cctcggcgtg
    12901 gcgccggccg cggtgaacct gaaggccacc accaccgagg gcctgggcgc catcggccgc
    12961 ggcgagggca tcgccgcgct ggcggtggcg ctcatcgagg aggcctgaga tgtattcgcc
    13021 cccttacaac caggtcgagg accgcgccga gctgctcgac ttcatgcgcg ccaacagctt
    13081 cgcgctgctg gtgaccgcca ccggcggcgc gccgctcgcc tcgcacctgc cggtgacgct
    13141 ggccgacggc gcgggcggca tcgtcatcca ctcgcacatg gcgcggaaca acccgcagtg
    13201 gcaggagttc ttcgaggacg aggtgctggt ggtcttcgcc ggcccgcacg cctacgtttc
    13261 gccgcgctgg tacgaagcga gggagcgcgt gccgacctgg aactacgccg cggtgcacgc
    13321 ttacggcacg gtgcaggtga ccgaggaccg cgcggtgaag cacgcggcgc agcgcgcgct
    13381 ggtggcggaa ctcgacccgc agtggctgcc gcaattcgac gccctgcgcc cggcgtacgt
    13441 gcagaacatg ctcgagggca tcgtcacctt cgacatcgcg gtgacgcgcc tggagacgcg
    13501 ctggaagctg tcgcagaatc gcggccggcg cgagcaggag ctgatcgccg ccgagctcga
    13561 gcgctcggcg gattcctcag cgcgcgcgct ggcggcgctg acccgcaagc attacgtcgc
    13621 ggagtagcag gccgcggggg cgggcgccgg cgagtcggcg gacgaggcgc atcgcgccca
    13681 gaaggaaatc tgccagggcg tcggcctggg cgagggcgct gcgggccgcc aggcgatcgg
    13741 ccggactcat gcggatttca tccagccgcg catacagcgc tgccaggccg ggattgctgc
    13801 ggacatgttt catgcaaaac tccttgtacg gcaggttgag ctgtcttttg ctgcgacgca
    13861 cgattaatgt gcatacgaag cttataagtg catatctttg tagaatatgc actacttaga
    13921 cagcttcctc cttccaagat tgcattgcag caaaaatcct gccgggccgc attgacccgg
    13981 gtcaagacgc cggcaggtac agccgcgcag ccaggccgcc gccttcccgg gggtccagct
    14041 cgaagcgccc gccgtgcgtg cgcgccacgc gttccacgat cgccagcccg agcccggcgc
    14101 cgccctggcc gctgcgcgcc gggtccaggc gcgcgaacgg gcgcttgagc cgctcggctt
    14161 cgccggcggg gatgccgggg ccgcggtcga gcacttcgat gagcacttcg ccgccggcgc
    14221 ggcgcgcctg cactgccacc ggctcgcccg cgtagcgcag cgcgttctcg atcaggttgc
    14281 cgacggcgcg gcgcagcgcc ttgggggcga aggcaaaggg ggcgaggtcg ggcgcggcga
    14341 cttccacctt gcggccgagg cgggcgtagt gctcgccgat ctcagccagc agcgcgccga
    14401 gcgaggccgc gccgcgcgcc tcgttgtcgc cgcgcgcgaa atcgaggaac tggccgagca
    14461 cccggtccat ctcctcgatg tcggcgttca tgccctcggc cgtcgcggcg tcggcgccgc
    14521 tcatttccac gcccaggcgc aggcgcgaca gcggcgtgcg caggtcgtgc gagatgccgg
    14581 cgaggaccat ggcgcgctcg cgctcgagcg actgcaggtc cccggccatg cggttgaact
    14641 ccgcctgcac cgtgcgcagc tccagttctc ccgccaccgg gagcggctcg ggcgtctcgc
    14701 catggcccag gcgccgcgcc gcggccgcca ggccggcgag cggccgcgag atgcgcgagg
    14761 cgatgagcca ggcgccgagc agcgccagcg ccagcagcgc cgcgccccag cccagccagc
    14821 ccaggccgaa ctccggctcg aagcgctcgc gcggcagcag cacccagaac tcgtcctcgt
    14881 cgatgaagaa gctcacccag aagccgcgca tgccgtcgcg cgcggcggcg aagcgcgtcg
    14941 ccgcgccgag ctgcgcgcgc acgctcgcgg cgacgcggtc caggcgaccc gccgggcgca
    15001 gcggccgcag cgcctcctcg ttggtcgccg ggtagatgcg gatgccctcg cgctcgttca
    15061 gctcgatcag caggtcgcgg cgcagcaccg ggtcggcgct caccagcgcg gcgcgcgtca
    15121 ggttgaccac gctcaccgcc tgctgcgcca gctcgcgctc gcgcggctcg cgctcgtagg
    15181 cccgcgagat ctggaacgag gcgaggaccg agaccaccag cagcagcgcg atcagcagga
    15241 acgagcgcca gaacagcgtc atcgcggcgt gccgtcgggg acgaacacgt agccgtggcc
    15301 ccacaccgtc tggatgtagc gcgggtgcgc cgggtcggcc tccaccagct tgcgcaggcg
    15361 cgagatctgc acgtcgatgg agcggtcgaa cacctcgtac tcgcggccgc gggccagctc
    15421 catgagctgg tcgcgcgaca gcggctggcg cgggcgctcc accaggactt tcagcacgga
    15481 gaattcgccg gtggtgagcg gcaccggctt gccgtccttc ttcagctctc gcgtggcggg
    15541 gtcgaactcg aacgcgccga agcggtgccg cccgtcctcg gacgagggcg cgccgggggc
    15601 gccggggcgg ggcctgcggc gcagcaccgc gttgatgcgc gccaccagct cgcgcgggtt
    15661 gaagggcttg ggcagatagt cgtcggcgcc catttccagg ccgacgatgc ggtccacctc
    15721 gtcgcccttg gcggtgagca tgatgatcgc cggcgagccg cccgcggcgc gcagccgccg
    15781 gcagatggcc agcccgtcct ccccggggag cattaggtcg agcaccagca ggtcgtagcg
    15841 ctcgcgcgac aggtacttgt ccatctcggg cgcgtcgcgc accgcgcgca cctcgaactt
    15901 ctgctcgccg aggtagcgcg tcagcaggtc gcgcaggcgc tggtcgtcgt cgacgacgag
    15961 gatgcgggtc ttcagttcgg ccatggcggg ttacgcaatg ttacaaaggg cgctgggcaa
    16021 acatcttaaa ctggcggcgc catgaaaacc ctccttgccc tgctgctggc ctgcgcggtg
    16081 tgcctgcccg cgctcgggca ggagcgcgcc gcgcgcgacc ggaacctgca gaaacagggc
    16141 ctgcagccgc acgagcgcat gcgcgaggaa cgccgcgagc tgcgccgcga gcggcccgac
    16201 cgcgcggagg ggcggcactt ctcgcgcgag gagcgccagc ggctgcgcca ggacctgatc
    16261 gacgccaacc ggtcgatgaa ggggcggcgc tagctagcgc aggtgggcga tcgagttggc
    16321 gtagtagcgc aggagcggca gctcctccgc ctgcgcgtag tacagcccct cgcgctcgcc
    16381 caccagccgc cgcaggcgca gcatgcgcag ccccacgttg accgcgtagt cgaagtcctt
    16441 gcgcggcacg tagacgtgcg cgccgcgcgc ctgcagctgc tccaccagcc ggctcacctc
    16501 ggatttcagc tccatctccg agatcgcctt gtcgccgtgc gcgacgaaca ccgtggcgat
    16561 gagcggcacc ggcagcaccg gcacgatgcg cccgaccgcg tccatcaggt ggcggccgag
    16621 cgcggccatc tcgcgcttgc gctcctcgcc cgcgagccgg tggaagtcga ggccgtgctg
    16681 gcggcagtac tcgcgcatcg acaccggcgc gccgaaattg acgcaggcgt agccgaagcg
    16741 gtgccattcg ctgcgcagca tcagcccgaa gttgtgcgcg gcgaagcgca gggtcgtcca
    16801 cagcgccgcg agccggccgc ggcgcgcggc atccgggtcg atcgagcgca ggagcgtgcg
    16861 gtcctccagc acgcggtcgt agttgatgcc gagcggcacg aacaccaggt cgcgctcgcc
    16921 gtcggcgcgg aagccgcgca tcatgtagtc gatggcgccg agcctgggtt cgcgcatgcg
    16981 cccgtcgcgc gtcaggccgc cttccgggaa caccgcctgc ggcacgccgg cttcggtcgc
    17041 catcacgatg tagcgctcca gcacgcgccg gtacagctcg tccttggaat tgcggcgcac
    17101 gaagtaggcg cccatcgagc ggatcagcgc cgacaggggc cagatgcgcg cccattcgcc
    17161 caccgcgtag gacagcgcgg tctggtcggc cgccaggtag ccggcgagca cgtagtccat
    17221 gttggagcgg tggttcatca cgaacaccac ggtggaatcg ggctcgacgc gctgcaggtt
    17281 ctccacgtcg acgtagccca ggcgcacgcg gtacagcagc tgcgccacgc gcttgccgaa
    17341 ccagtagccg atgcggaagt agaggtaggc gttgaacgcc ggcacggtct cgcgcgcgta
    17401 gcgctccacc agctcgagcg ccaccagcac cgggaccttg ttctgcgcgg cgaactgctc
    17461 ggccgcctgc tgcactttcg ggtcggtgag caggcggtgg atcagcgcct gccgccgcgt
    17521 gcgctggaac gggcggatgc cgatgcgcag ccgcttgccg acctcgtcga tcacctggtt
    17581 ggccgggtgc gtcaccagcc agcgcagcgc cggcatcagc gcgtgctcgt acagcgccca
    17641 cagcgccagc agcgcgccgg cgagcgccag ccacagaggt accgcgacgg aatccgccat
    17701 gccccctccc tgggggaagt ctagcctagc ggcggcgccg ctcgcgcgag aacaggtaca
    17761 gcccgctggc gatgacgatg gcggcgccga tccacaccgc gggatccggc acgctgccga
    17821 acaccaggta accgaacagc gccatgtaca ggatctgctg gtagaggaac ggcgccagcg
    17881 tgctcgccgg cgcgtagcgg tgcgccatgg cgaggaggta gtgccccgtg ccgccgaaca
    17941 cccccatcag gcacagcagc agccactcga accagcctgg cggcgactgc caggcagcga
    18001 gcgcgaacgg cgccagcacc acgctcgcca ccaccgcggg caggaactgg atggtctcgg
    18061 gcggatcgta ggccgcgagc ttgcgcgtca tcatgttgaa cgccgcgtac agcacggcat
    18121 tggccacgga gagcagcatt gcgggatgga aggactcgga gcccgggcgc acgatcacca
    18181 gcaccccggc gaagccgacc aggatcgccg cccagcgctt ggcgtcgagt ttttcgccga
    18241 gcagcggcgc cgcgccgagc gccaccagca gcggcaccat gaagaagatc gacgcggtga
    18301 cggtcaactg caggaactgc agcgcccaga agttcatccc ggtcatggcg acgaacatca
    18361 ggccgcgcac gaggtgccag cgcaggtggc tggtgcgcac cagcgcgcgg ccgcggatcg
    18421 ggaacagcag ggccgaggcg atcaccacct gcgagaggaa gcgcagccag accaccacca
    18481 gcaccggcac cgtctgcacc agccatttcg cgctgccgtc gagcagcgtg aacaggacgt
    18541 aagtgagcga gatgaggccg atgccggtga ggcggttcct cgcgctgtcg gcctgcagca
    18601 ctacagcgcg cgcttgaccg cgctggtcac cgcgcgcgtg tcgtgcgcgc cgccgaggtc
    18661 cggcgtgaac acctgctcct cggtggtgcg ctcgatcgcc gccatcagcc gggcggcggc
    18721 cttcggctcg cccaggtgct cgagcatcag cgccgcggtc cagaaggtgg cgatggggtt
    18781 ggcgacgccc ttgccggtga tgtcgaaggc cgagccgtgg atcggctcga acatcgaggg
    18841 gaatttccgc tcgggattga ggttcgccgt cggcgcgatg ccgagcgagc cgctcaacgc
    18901 cgccgccagg tccgacagga tgtcggcgtg caggttgctg gcgacgatgg tgtcgagcga
    18961 cccgggcttc aacaccatgc gcgtggtcat ggcatccacc aggatgcggt cggtcttcac
    19021 ctcgggatac ttcttcgcca cctcgtagaa gatctcgtcc cacagcacca tgccgtggcg
    19081 ctgggcattg gacttggtga cgacggtcag cagcttgcgc ggccgcgacc gggcgaggtc
    19141 gcaggcgtag cgggcgatgc gctcgacccc ggcgcgggtg aacaccgcga catccatgcc
    19201 gacctcgatc ggcagaccgc gatgggcgcg gccgccgacg ccggcatact cgccctcgct
    19261 gttctcgcgc acgatcaccc agtcgatgcc ggcgcccagc tccggccgca gcggcccgat
    19321 gacgcccggc agcagctttg acggacggac at
//
LOCUS       DASRIE010000254         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_2340543, whole genome shotgun sequence.
ACCESSION   DASRIE010000254 DASRIE010000000
VERSION     DASRIE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340543"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..885
                     /locus_tag="VFB53_11105"
     CDS             109..885
                     /locus_tag="VFB53_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN87319.1"
                     /translation="MLALYRELEGTTYLLLYAIRRLGVLRLPPVMEELVRQIYFTGAA
                     ATGGIVLRGAFIGTLIIAYVIEVLHADAELAVKLLLWVVLREVGPLIAAVLVIQRSGT
                     AIATELALRTITGEIDSLRRMRIDPRDYLVVPRVVATAVSNAALTLYVQMIAVGGGMV
                     LSSIMINVPLGQMLGTFFELASPLDMMYSLIKSLLFGAAIAGIACYHGLNPGGRSINA
                     VPPATIRAVTEALLSVMLLNVVFAYLVYGVALWGLVTVEQ"
     gene            882..1472
                     /locus_tag="VFB53_11110"
     CDS             882..1472
                     /locus_tag="VFB53_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN87320.1"
                     /translation="MSVVLGYEPPAGGEEAARVWVEAPGTHRVAAACKDELIEAMHHL
                     EPPAGARLVVLGTPVDYLGEAERAALRARVGFVPVHGGLISNLNAWENVSLPVGFHEP
                     ARLSGVPAEVHALLEEIGGVEPNLLAKLPEHMTPYEKRLVAYIRALLERPELLLVENP
                     AAGLGPTKRKRVARFAEAYHARRPGGTYVQLDDSPG"
     gene            1493..2491
                     /locus_tag="VFB53_11115"
     CDS             1493..2491
                     /locus_tag="VFB53_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN87321.1"
                     /translation="MSDKLATPRRFRGIRKKISGFLLTAVVVFVGVIALVGYKQGLFV
                     KHTHVYFHAPDASGINKGMSVRLYGVPVGAVKDIEILERGGVRVRIGINTDYLPLMPR
                     GSQARLVREGVVGAASIQIVSSPTSLDRTPVAEGEVLKFIPHRSMNEMVDELRQQMAP
                     AFQELRKAVAEMTDPQGDFRRSVSSVREMIEELPGTTRDLRQLLRDTDRTMIALGRQA
                     DTVGRQADAALAVITRVGLQTEEHLPVLAGKLATTLDSLNATSEQLRVTTRENGEALR
                     ELLAQAPELMRGGGELVRDSQELAAAARRSWLLRDYVEPLEMRTLPVDSFESFGKR"
     gene            2488..3348
                     /locus_tag="VFB53_11120"
     CDS             2488..3348
                     /locus_tag="VFB53_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN87322.1"
                     /translation="MRVWLQAGLAVAALLAGCSSAPPSASTVAARQSAAIEANRKGEA
                     YVRHGELDNAARSYGEALRLSQSLEDPAGIAANAINLSIVRQRQGRFADARTSLALIT
                     EEHFPPGRLAEAALRHALLDMDELRFASAQQWADKASNHCATRCAVAAAIHNVRAQLA
                     LQSGQTEAAVAAARAAQEASRSANDRAELANALRLLGIAALRSGDATAARTHLEQALV
                     LDRELGASRKIALDLLALGRAAALGGDREAAKAYYARALAVAEADRDPPGAAEARALL
                     DGVDKAATGK"
     gene            complement(3492..>3932)
                     /locus_tag="VFB53_11125"
     CDS             complement(3492..>3932)
                     /locus_tag="VFB53_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZN87323.1"
                     /translation="IKGPGQYFGEMVLDEQPRSASVMTLEPATFAVLSRADFRGFLLA
                     HPAVALQLIQNLIRVARGLNQNVRSLAMLDVYGRVARILTELAVERDGKMVIPDKLTQ
                     KDIAARVGASREMINRILRDLTTGGYVSVEDGRITINKTPPARW"
BASE COUNT          582 a         1399 c         1380 g          571 t
ORIGIN      
        1 tcggcgagcg ctggtcggag gacagcgacc ggctgcacgc gtggggatac ctgagggaag
       61 cgtgaagcag taagattcct gcagaaatca cccgaggggg acggcgggat gctcgccctc
      121 taccgcgagc tcgaaggcac cacctatctg ctgctgtacg ccatccggcg gctgggcgtg
      181 ctgcgcctgc cgccggtgat ggaagagctg gtgcgccaga tctacttcac cggcgcggcc
      241 gcgaccggcg gcatcgtgct gcgcggcgcc ttcatcggca cgctgatcat cgcttacgtg
      301 atcgaggtgc tgcacgccga cgccgagctc gcggtgaagc tcctgctgtg ggtggtgctg
      361 cgcgaggtcg ggccgctgat cgcggcggtg ctggtgatcc agcgcagcgg caccgcgatc
      421 gccaccgagc tcgcgctcag gaccatcacc ggtgagatcg acagcctgcg gcgcatgcgc
      481 atcgatccgc gcgactacct ggtggtgccg cgggtggtcg ccacggcggt gtcgaacgcc
      541 gcgctcaccc tctacgtgca gatgatcgcg gtcggcggcg gcatggtgct gtcgtcgatc
      601 atgatcaacg tgccgctcgg ccagatgctg ggcaccttct tcgagctggc gagcccgctc
      661 gacatgatgt actcgctcat caagagcctg ctgttcggcg cggcgatcgc cgggatcgcc
      721 tgctatcacg ggctgaatcc cggcggcagg tcgatcaacg ccgtgccccc ggcgacgatc
      781 cgcgcggtga ccgaggcgct gctgagcgtg atgctgctca acgtggtgtt cgcctacctg
      841 gtctacggcg tggcgctctg gggcctggtg acggtggaac aatgagcgtg gtcctcggct
      901 acgaaccccc cgccggcggc gaagaagcgg cgcgcgtgtg ggtcgaggcg ccaggcacgc
      961 accgcgtcgc cgccgcctgc aaggacgagc tgatcgaggc gatgcatcac ctcgagccgc
     1021 ccgccggcgc ccgcctcgtg gtcctcggca cgccggtcga ttacctgggc gaggcggagc
     1081 gcgccgcgtt gcgcgcccgc gtcggcttcg tgccggtgca cggcggcctg atcagcaacc
     1141 tgaacgcgtg ggagaacgtc tcgctgccgg tgggctttca cgagccggcg cggctctccg
     1201 gcgtgccggc cgaggtgcac gcgctgctcg aggaaatcgg cggcgtggag ccgaacctgc
     1261 tcgccaagct gccggagcac atgacgccat acgaaaagcg gctggtcgcc tatatccgcg
     1321 cgctgctcga gcggcccgag ctcctgctgg tggaaaatcc tgcggcgggc ctcggcccga
     1381 ccaagcgcaa gcgcgtcgcg cgcttcgccg aggcctatca tgcacgccgc cccggcggca
     1441 cctacgtgca gctcgacgac tcgccgggct gacccatcca agggagaaca ccatgagcga
     1501 caaactggcc acgccccgcc gcttccgggg aatccgcaag aagatcagcg gcttcctgct
     1561 gaccgcggtc gtcgtgttcg tcggcgtgat cgcgctggtc ggctacaagc agggactgtt
     1621 cgtgaagcac acgcacgtct acttccatgc gcccgacgcg agcggcatca acaagggcat
     1681 gtcggtgcgc ctgtacggcg tgcccgtggg cgcggtcaag gacatcgaga tcctcgagcg
     1741 cggcggcgtg cgggtgcgca tcggcatcaa caccgactac ctgccgctga tgccgcgcgg
     1801 ctcgcaggcg cggctggtgc gcgaaggcgt ggtcggtgcg gcctcgatcc agatcgtctc
     1861 cagccccacg tcgctcgatc gcaccccggt cgcggagggc gaggtgctca agttcatccc
     1921 ccaccgcagc atgaacgaga tggtcgacga gctgcgccag cagatggcgc cggcgttcca
     1981 ggaactgcgc aaggcggtcg ccgagatgac cgacccgcag ggcgacttcc gccgctcggt
     2041 gagctcggtg cgcgagatga tcgaggagct gcccgggacg acccgcgacc tgcgccagct
     2101 gctgcgcgac accgaccgca ccatgatcgc gctcggccgg caggccgaca ccgtcggcag
     2161 gcaggcggac gcggcgctcg cggtgatcac ccgcgtgggc ctgcagaccg aggagcacct
     2221 gccggtgctc gcgggcaagc tcgccaccac gctcgactcc ctcaacgcca cttccgagca
     2281 gctgcgcgtc accacgcgcg agaacggcga ggcgctgcgc gagcttctcg cccaggcgcc
     2341 ggagctgatg cgcggcggcg gcgagctggt gcgcgacagc caggagctcg cggccgcggc
     2401 gcgccgctcg tggctgctgc gcgactacgt cgagcccctg gagatgcgca cgctgccggt
     2461 cgacagcttc gagtccttcg gcaagcgatg agggtctggc ttcaggcggg actcgccgtt
     2521 gccgccctgc ttgcagggtg ctcgtcggcg ccgccgtccg cgtccaccgt cgccgcgcgc
     2581 cagagcgcgg cgatcgaggc gaaccgcaag ggcgaggcct acgtgcggca cggcgagctc
     2641 gacaacgccg cgcgcagcta cggcgaggcg ttgcgcctgt cgcagtcgct ggaggacccc
     2701 gccgggattg ccgccaacgc gatcaacctg tcgatcgtgc gccagcgcca gggccgcttc
     2761 gccgatgcgc gcaccagcct ggcgttgatc accgaggagc atttcccccc ggggcgcctc
     2821 gccgaggcgg cgctgcgcca cgccctgctc gacatggatg agcttcggtt cgcatccgcc
     2881 cagcaatggg ctgataaggc ttccaaccac tgtgcgacac gctgcgctgt ggcggcggcg
     2941 atccacaacg tccgcgccca gctcgccctg cagtccggac agaccgaagc cgcggtcgcc
     3001 gcggcgcgtg cggcccagga ggcgagccgc tcggcgaacg accgcgccga gctcgccaac
     3061 gctctgcgcc tcctcggcat tgcggcgctg cgcagcggcg acgctaccgc cgcccgcacc
     3121 catctcgagc aggcgctggt gctggaccgc gaactcggcg cctcgcgcaa gatcgcgctc
     3181 gacctgctcg ccctcggccg cgccgccgct ctcggcggcg accgggaagc ggccaaggcc
     3241 tactacgccc gcgcgctcgc cgtcgccgag gccgaccgcg accctcccgg cgccgccgaa
     3301 gcccgcgccc tcctcgacgg cgtcgacaag gccgccaccg gcaaataaaa aagggccgct
     3361 cgcgcggccc ggaggaggag tcttgaatgc aatgccactg tagacagcgc gcgcctgcgc
     3421 cgccagcgag aaaggcacgg ttgggtagtg cggtcttcac atccccgccc ccgccgtgcg
     3481 gtatttggcg cctaccagcg cgccggcggg gtcttgttga tggtgatgcg gccgtcctcg
     3541 acgctgacgt agccgccggt ggtcaggtcg cgcaggatgc ggttgatcat ctcgcgcgag
     3601 gcgccgacgc gcgcggcgat gtccttctgc gtcagcttgt ccgggatcac catcttgccg
     3661 tcgcgctcca ccgccagctc ggtcaggatg cgcgccacgc ggccgtagac gtcgagcatc
     3721 gccaggctcc tgacgttctg gttcaggccg cgcgcgacgc ggatcaggtt ctggatcagc
     3781 tgcagcgcca ccgcggggtg cgcgaggagg aagcctctga agtcggcgcg cgacaagacc
     3841 gcgaaggtcg ccggctcgag cgtcatcacc gaggccgagc gcggctgctc gtcgagcacc
     3901 atctcgccga agtactggcc cgggcccttg at
//
LOCUS       DASRIE010000255         7949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10102
            k141_505994, whole genome shotgun sequence.
ACCESSION   DASRIE010000255 DASRIE010000000
VERSION     DASRIE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963630
            Sequence Read Archive: SRR5582390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:34:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,210
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505994"
                     /isolate="SMAG_U10102"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFB53_11130"
     CDS             <1..171
                     /locus_tag="VFB53_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN87324.1"
                     /translation="GVDPVQLYISIAGLGYFYLSNRHTLSTIFERDLLAPRSKAGRLQ
                     HMTELVLGYLRR"
     gene            176..1006
                     /locus_tag="VFB53_11135"
     CDS             176..1006
                     /locus_tag="VFB53_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN87325.1"
                     /translation="MRIALCNEVIAPMPFPQQCVYAAKLGYDGLEIAPYTLSEEPQRL
                     GSAQLAAARTAAQDAGIAITGLHWLLVKPAGLSISTRDAAVRAKTLDLMCVLVEQCAE
                     LGGRYLVHGSPHQRRIDAGDTREAALDRARECFARVAERAAKAGVLYCLEPLAAEQTP
                     LVNTLAEAAAMVAGIGNPALRSMLDCSSAGRMEQETLAALVDRWLPRGVIAHVQVNDR
                     NRRGPGQGEQKFAPLFAALKRHGYGGDIAVEPFDYVPDGPGAAARAIGYLRGILEALP
                     "
     gene            1003..1659
                     /locus_tag="VFB53_11140"
     CDS             1003..1659
                     /locus_tag="VFB53_11140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZN87326.1"
                     /translation="MSPKLPCPRPGRTVLEGRYARLEPVAPAHAAGLLAASAAAKSFD
                     YLFDVPPQGAQDILAWIARNAAAEDLMVHAVVDKATGVAGGRQSYMRITPEHGVIEIG
                     NILWGPAIRRTRVATEALYLAAKHVFEDLGYRRFEWKCNDLNEPSKAAAKRFGFVFEG
                     VFRQHMWVKGANRDTAWFAMLDRDWPRLRAAYARWLDPANFDAAGRQRAKLEVPISGA
                     "
     gene            complement(1643..2620)
                     /locus_tag="VFB53_11145"
     CDS             complement(1643..2620)
                     /locus_tag="VFB53_11145"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN87327.1"
                     /translation="MKSLIAAVFAALALPAAAQDKFTLQLNWFHLADHSPIYMALKKG
                     YYKEENIELTVLRGSGSADSAKKIDLKQGDLGISDAPTVLTAISKGADLRMVAVVFDK
                     AGNNAFFKKSANIRSPKDLVGRKIAAPPADSHRVLWPAFAAINKLDPNGVTLVNVKPE
                     GKQAIVAAGEVDASFDLYTSYAIWEKVLGKGNVGHLLWADYGLPIYGHTYFVNRELEK
                     KNPRLIERFLRATHKGWRDAHANPREAIDAMAASVPGLDSESLFNTTPQIMDLCITER
                     SRRHGIGWIEKDLMQRTIDITFAQNKPDRPVNVDDVFTNAYSSKIKPLK"
     gene            complement(2631..3419)
                     /locus_tag="VFB53_11150"
     CDS             complement(2631..3419)
                     /locus_tag="VFB53_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN87328.1"
                     /translation="MTSQERGLAGAVGYYVPSALVMLGLLVAWQLAVDLGGVKEYILP
                     SPTAAIRTYLLPNYQWANNFLATFYATMGAFALSAVLGIGLAVVIVWNEMLMRTVMPV
                     LVLFNTLPKIALAPLFVIWLGYGIWPNIVIGTTIAFFPMVVNTAVGLATAEPEMLELV
                     RSLKASRWQIFAKIRFPNALPYIFTGLKLNASMSVIGAIVGEFVASERGLGALIITGG
                     VTLQTPSIFASLILISVLGLALYGAVVAVERMVAPWVYREQTVN"
     gene            3468..4865
                     /locus_tag="VFB53_11155"
     CDS             3468..4865
                     /locus_tag="VFB53_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN87329.1"
                     /translation="MSPRQAAPRFRVTAIDLLERDVRLRMPFRFGVVTLTESPQAFVR
                     ARIRLDDGREAAGAAAELLAPKWFDKDPALSNADNYDQLRASLARARAQYLAHEPRTA
                     WAHATPGKGLVENYGPALLDRALLDALCRALGVPFYRAVQTNLVGAGSFEGLDLGAFF
                     ATLRPGAAIAARHTVGLVDPITAAGREQRVGDGLPETLEEVVARYGQRYFKLKVAGEA
                     RADVERLAAIAAVLDRIEGPYHASLDGNEQYEDLQGVAELWARMRAEPRLRRLVDSIL
                     FIEQPVKRSRALERRVEGIDKPVIIDESDDALDAFPRAKALGYRGVSSKTCKGLYKSL
                     INRARCAAWNGKMGSGKMGSDPFFFMSGEDLTIQAGLALQQDTALVSLLGLTHLERNG
                     HHYVNGMAGLPQGEQDAFLAAHPDLYERSHGAVRVKIAGGMLSIGSLDCPGYASGAMP
                     DWTTMREMRLQKEPA"
     gene            4862..6010
                     /locus_tag="VFB53_11160"
     CDS             4862..6010
                     /locus_tag="VFB53_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN87330.1"
                     /translation="MSTRRIGIVMHGVTGRMGMNQHLLRSILEIRRQGGVALQDGTRL
                     VPDPLLVGRNAAKLGEVARQHGVQRWTTDLAAALASKEDSVFFDAASTQLRPQLLKRA
                     IRAGKHVYCEKPVATTLAEAQGLYRAAKKAKVKHGVVQDKLWLPGLIKLARLRDAGFF
                     GKILSVRGEFGYWVFEGDRQAAQRPSWNYRKQDGGGIILDMLCHWRYVLDNLFGEVQS
                     VSCLGATHIPQRWDEAGKKYRATADDAAYATFRLKGGAIAHFNSSWTVRVRRDDLLTL
                     QVDGTEGSAVAGLRRCLVQSYADTPKPVWNPDIPQPIDFFDGWKELPDDAADNAFKVQ
                     WEMFLRHVAGEGPFRWGLLEGAKGVQLAELGLKSWKQRKWIDIPKLKA"
     gene            5980..7155
                     /locus_tag="VFB53_11165"
     CDS             5980..7155
                     /locus_tag="VFB53_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN87331.1"
                     /translation="MDRHPQAEGLSVGLPAAGGALEPYRMLGPRQFARPARPFTRRAL
                     AAAHVVADPLSARDPWLEPAVDWDATLAYRRHLWSWGLGLAEAMDTAQRGMGLDWANS
                     LELIRRTLDAARDVEGAVVFSGAGTDHVAKADVAGVITAYEEQCAAIEKLGGRIILMA
                     SRALAASARSPEDYGRVYERILGQVRQPVILHWLGEMFDPALEGYWGRKDHAAAMDVC
                     LDVIRRNAAKVDGIKISLLDKDKEIAMRRRLPAGVRMYTGDDFNFAELIEGDAAGHSD
                     ALLGIFDAIAPAASAALAALAAGDARSYHDILGPTVPLSRHVFRAPTRYYKTGVVFLA
                     WLNGHQEHFVMVGGQQSARHILHFAELFRLADAAGLLADPPLACARMKQLLALHGCG"
     gene            7197..>7949
                     /locus_tag="VFB53_11170"
     CDS             7197..>7949
                     /locus_tag="VFB53_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZN87332.1"
                     /translation="MQRRLFLAAVAAAFALPVGAQTITLHGASQFSDEHAFTKSMVRF
                     EELVKKYYGKPVNFVLHKNSELGLEKQYFEYMAQGKAVDYAIVSPAHMSTFSKAAPFI
                     DAPFLFRDLKHWNKVLDADILKPVADEIAQKADVMLVGYAGGGTRNIFVNKPVRNMAE
                     LKALKVRVQGAPIWSKTFQAAGMAPTVIAYNEVYNAIQNGVIAAGENEAAGVEQMKFY
                     EVAPHLSMTQHAITIRPLCFSGKTFKKLDKDLQ"
BASE COUNT         1261 a         2684 c         2817 g         1187 t
ORIGIN      
        1 ggcgtcgacc cggtgcagct gtacatctcg atcgccgggc tgggctactt ctacctcagc
       61 aaccggcata ccctgtcgac gatcttcgag cgcgacctgc tcgcgccgcg gagcaaggcg
      121 ggacgcctgc agcacatgac cgagctggtg ctcggctatc tgaggaggta acgccttgcg
      181 catcgcgctg tgcaacgagg tgatcgcgcc gatgccgttt ccgcagcaat gcgtgtacgc
      241 ggcgaagctc ggctacgacg gcctggagat cgcgccctac acgctgtcgg aggagcccca
      301 gcgcctgggc agcgcgcagc tcgcggcggc gcgcacggcg gcgcaggacg cgggcatcgc
      361 catcaccgga ctgcactggc tgctggtgaa gccggccggc ctctcgattt ccacgcgcga
      421 cgccgcggtg cgcgcgaaga ccctggacct gatgtgcgtt ctggtggagc agtgcgcgga
      481 gctcggcggc aggtacctgg tgcacggctc gccgcaccag cggcgcatcg acgccggcga
      541 cacgcgcgag gcggcgctcg accgcgcgcg cgaatgcttc gccagggtcg ccgaacgcgc
      601 ggccaaggcg ggcgtgctgt actgcctgga accgctggcg gccgagcaga ccccgctggt
      661 gaacacgctg gcggaggccg cggcgatggt ggccggcatc ggcaacccgg cgctcaggag
      721 catgctcgat tgcagctcgg ccgggcgcat ggagcaggag accctcgccg cgctggtgga
      781 ccggtggctg cccaggggcg tgatcgcgca cgtgcaggtg aacgaccgca accggcgcgg
      841 gccggggcag ggcgagcaga agttcgcgcc gctgttcgcc gcgctcaagc gccacggcta
      901 cggcggcgac atcgcggtgg agcccttcga ctacgtgccc gacgggcccg gggcggcggc
      961 gcgcgccatc ggctacctgc gggggatcct ggaggcgctg ccgtgagccc gaaactgccc
     1021 tgcccgcggc ccgggcgcac cgtcctggaa gggcgctacg cgcgcctgga gccggtggcg
     1081 ccggcgcacg cggcaggcct gctcgccgcg tccgcggcgg cgaagagctt cgactatctg
     1141 ttcgacgtgc cgccgcaagg cgcgcaggac atccttgcct ggatcgccag gaatgccgcg
     1201 gccgaagacc tgatggtgca tgccgtcgtg gacaaggcca ccggcgtcgc cggcggacgg
     1261 cagtcctaca tgcgcatcac ccccgagcac ggcgtgatcg agatcggcaa catcctgtgg
     1321 gggccggcga ttcggcgcac gcgcgtcgcc accgaggcgc tgtacctggc ggcgaagcac
     1381 gtgttcgagg acctgggcta ccggcgcttc gagtggaagt gcaatgacct gaacgagcct
     1441 tcgaaggccg ccgcgaagcg cttcggcttc gtcttcgaag gcgtgttccg ccagcacatg
     1501 tgggtgaagg gcgcgaaccg cgacaccgcc tggttcgcga tgctcgaccg cgactggccg
     1561 cgcctgcgcg cggcctacgc gcgctggctc gacccggcga acttcgacgc cgccgggcgc
     1621 cagcgcgcga aactcgaggt gcctatttca ggggcttgat tttcgagctg taggcgttgg
     1681 tgaacacgtc gtccacgttc accggccggt cgggcttgtt ctgcgcgaag gtgatgtcga
     1741 tcgtgcgctg catcaggtcc ttctcgatcc agccgatgcc gtgccggcgc gagcgctcgg
     1801 tgatgcacag gtccatgatc tgcggcgtgg tgttgaacag cgactcgctg tccagccccg
     1861 gcaccgaggc ggccatggcg tcgatcgcct cgcgcgggtt ggcgtgggcg tcgcgccagc
     1921 ccttgtgcgt ggcccgcagg aaccgttcga tcagcctggg gttcttcttt tccagctcgc
     1981 ggttgacgaa ataggtgtgg ccgtagatcg gcaggccgta gtcggcccac agcaggtgcc
     2041 ccacgttgcc cttgcccagc accttctccc agatggcgta gctggtgtag aggtcgaacg
     2101 aggcatccac ttcgcccgcg gcgacgatcg cctgcttgcc ttcgggtttc acattcacca
     2161 gggtgacgcc gttggggtcg agcttgttga tggcggcgaa cgccggccac agcacgcgat
     2221 gcgagtcggc gggcggggcg gcgatcttcc tgccgaccag gtccttgggc gagcggatgt
     2281 tggcgctctt cttgaagaag gcgttgttgc cggccttgtc gaacaccacc gccaccatcc
     2341 tcaggtcggc gcccttggag atcgcggtga gcacggtggg cgcgtccgag atgcccaggt
     2401 cgccctgctt caggtcgatc ttcttcgcgc tgtccgcgga gcccgagccg cgcagcacgg
     2461 tgagctcgat gttctcttcc ttgtagtagc ccttcttcag ggccatgtag atcggcgaat
     2521 ggtccgccag gtggaaccag ttgagctgca gggtgaactt gtcctgcgcc gcggcgggca
     2581 gggcaagggc ggcaaagacg gcggcgatca gcgatttcat ccgttcctcc tcagttgact
     2641 gtctgttccc ggtataccca gggcgcgacc atgcgctcga cggcgacgac cgcgccgtac
     2701 agcgccaggc ccagcaccga aatcaggatc agcgacgcga aaatcgacgg cgtctgcaag
     2761 gtgacgccgc cggtgatgat cagcgcaccc aggccgcgct cggaggcgac gaactcgccg
     2821 acgatggcgc cgatcacgct catcgaggcg ttcagcttca ggccggtgaa gatgtagggt
     2881 agcgcattcg gaaagcggat cttggcgaat atctgccagc gcgaggcctt gagggacctc
     2941 accagctcga gcatctcggg ctcggcggtg gcgagtccca ccgcggtgtt caccaccatg
     3001 ggaaagaacg cgatggtggt cccgatcacg atgttgggcc agatgccgta gccgagccag
     3061 atcacgaaca gcggggcgag ggcgatcttc ggcagcgtgt tgaacagcac cagcaccggc
     3121 atcacggtgc gcatcagcat ctcgttccac acgatcacca cggcgaggcc gatgccgagc
     3181 acggcggaca gcgcgaaggc gcccatcgtg gcgtagaagg tggcgaggaa gttgttggcc
     3241 cactggtagt tcggcaggag gtaggtcctg atcgccgccg tgggcgaggg caggatgtac
     3301 tccttcacgc cgcccaggtc caccgccagc tgccaggcga cgagcaggcc gagcatcacc
     3361 agcgcgctcg gcacgtagta gccgacggcg ccggcgagcc cgcgttcttg actagtcatc
     3421 gctgtcccgc tcagaattaa cgaaacggtt aaatcatagg aacgcctttg tctccgcgtc
     3481 aagccgcgcc gcgattccgc gtcacggcaa tcgacctgct ggaacgcgac gtgcggctgc
     3541 gcatgccgtt ccgcttcggc gtggtgaccc tcaccgagtc gccgcaggcc ttcgtgcgcg
     3601 cgcgcatccg cctggacgac ggtcgcgagg ccgccggggc ggccgccgag ctgctggcgc
     3661 ccaagtggtt cgacaaggac ccggcgctca gcaatgcgga caactacgac cagctgcgcg
     3721 cctcgctcgc tcgcgcgcgc gcgcagtacc tcgcgcacga gccgcgcacg gcctgggcgc
     3781 atgcgacgcc cggcaagggg ctggtggaga actacggccc ggcgctcctc gaccgcgcgc
     3841 tgctcgacgc gctgtgccgc gcgctcggcg tgcccttcta ccgcgcggtg cagacgaacc
     3901 tggtgggcgc cggcagcttc gaggggctgg acctcggcgc cttcttcgcc acgctgcggc
     3961 cgggcgcggc gatcgccgcg cgccacacgg tggggctggt ggacccgatc accgccgccg
     4021 gccgggagca gcgcgtcggc gacggcctgc ccgagacgct ggaagaagtg gtggcgcgct
     4081 acggccagcg ctatttcaag ctgaaggtgg cgggcgaggc gcgcgccgac gtggagcggc
     4141 tcgcggcgat cgccgcggtg ctcgaccgga tcgagggccc ctaccacgcc tcgctcgacg
     4201 gcaacgagca gtacgaggac ctgcagggcg tggccgagct gtgggcgcgc atgcgcgccg
     4261 agccgcggct gcgccgcctg gtggactcga tcctgttcat cgagcagccg gtgaagcgca
     4321 gccgcgcgct cgagcgccgg gtcgagggca tcgacaagcc ggtgatcatc gacgagtccg
     4381 acgatgcgct cgacgccttc ccgcgcgcca aggccctcgg ctaccgcggc gtgtcgtcga
     4441 agacctgcaa gggcctgtac aagtccctga tcaaccgcgc acgctgcgcc gcgtggaacg
     4501 ggaaaatggg gtccgggaaa atggggtcag accccttttt cttcatgtcg ggcgaggacc
     4561 tcaccatcca ggcgggcctc gcgctgcagc aggacacggc gctggtgtcg ctgctggggc
     4621 tcacgcacct ggagcgcaac ggccaccact acgtgaacgg catggcgggg ctgccgcagg
     4681 gcgagcagga cgcgttcctc gccgcgcacc cggacctgta cgagcgcagc cacggcgcgg
     4741 tgcgcgtgaa gatcgccggc ggcatgctgt cgatcggctc gctcgactgc ccgggctacg
     4801 ccagcggcgc gatgcccgac tggaccacca tgcgcgagat gcgattgcag aaggaaccgg
     4861 cgtgagcacg cggcgcatcg gcatcgtcat gcacggcgtc accgggcgca tgggcatgaa
     4921 tcagcacctg ctgcgctcga tcctggaaat ccgccggcaa ggcggcgtcg ccctgcagga
     4981 cggcacgcgc ctcgtgcccg acccgctgct ggtgggccgc aacgcggcga agctcgggga
     5041 ggtggcgcgc cagcacggcg tgcagcgctg gaccacggac ctcgccgcgg cgctcgcctc
     5101 gaaggaggac agcgtgttct tcgatgcggc gtccacgcaa ttgcggccgc agctcctcaa
     5161 gcgggcgatc cgggcgggca agcacgtcta ctgcgagaag ccggtcgcca ccacgctcgc
     5221 cgaggcgcag ggcctctacc gcgcggcgaa aaaggcgaag gtgaagcacg gcgtggtgca
     5281 ggacaagctc tggctgcccg ggctgatcaa gctcgcgcgc ctgcgcgacg cgggcttctt
     5341 cggcaagatc ctttccgtgc gcggcgaatt tggctactgg gttttcgaag gtgaccgcca
     5401 ggccgcgcag cgcccgtcgt ggaactaccg caagcaggac ggcggcggga tcatcctcga
     5461 catgctgtgc cactggcgct acgtgctgga caaccttttc ggcgaggtgc agtcggtgag
     5521 ctgcctcggc gccacgcaca tcccgcagcg ctgggacgag gcggggaaga aatacagggc
     5581 gacggcggac gacgccgcct atgccacctt caggctgaag ggcggcgcca tcgcgcactt
     5641 caactcctcc tggacggtgc gggtgcggcg cgacgacctg ctcacgctgc aggtggacgg
     5701 caccgagggc agcgcggtgg cgggattgcg ccgctgcctc gtgcagtcct acgccgacac
     5761 gcccaagccg gtgtggaatc cggacatccc gcagccgatc gatttcttcg acggctggaa
     5821 ggaactgccc gacgacgccg ccgacaacgc cttcaaggtg caatgggaga tgttcctgcg
     5881 gcatgtggcc ggcgagggcc cgttccgctg ggggctgctc gagggcgcca agggggtgca
     5941 gctcgccgag ctcggactga agagctggaa gcagaggaaa tggatcgaca tccccaagct
     6001 gaaggcctga gcgtcgggct gccggccgcc ggcggcgcgc tcgagcccta ccgcatgctc
     6061 ggcccgcggc aattcgccag gccggcgcgg cccttcacgc gccgcgcgct cgccgccgcg
     6121 cacgtggtgg ccgatccgct gtcggcgcgc gacccctggc tcgagccggc cgtcgactgg
     6181 gacgccacgc tcgcctaccg gcgccacctg tggtcctggg gcctcggcct cgccgaggcg
     6241 atggacaccg cgcagcgcgg catggggctg gattgggcga actcactcga gctcatccgg
     6301 cgcacgctgg atgcggcgcg cgacgtggag ggcgcggtgg tcttctccgg cgcgggcacc
     6361 gaccatgtgg cgaaggcgga cgtcgccggc gtgatcaccg cctacgagga gcagtgcgcg
     6421 gcgatcgaaa agctgggcgg gcgcatcatc ctgatggcga gccgcgccct cgccgcgagc
     6481 gcgcgctcgc ccgaggacta cggccgggtc tacgagcgca tcctcggcca ggtgcggcag
     6541 ccggtgatcc tgcactggct gggcgagatg ttcgacccgg cgctggaggg ctactggggc
     6601 aggaaggacc acgccgcggc gatggacgtg tgcctggacg tgatccgcag gaacgccgcg
     6661 aaggtggacg gcatcaagat ctcgctgctg gacaaggaca aggagatcgc catgcgccgc
     6721 cggctccccg ccggcgtgcg catgtacacc ggcgacgact tcaacttcgc cgagctgatc
     6781 gagggcgacg ccgcggggca ctccgacgcg ctgctcggca tcttcgatgc gatcgcgccg
     6841 gcggcctcgg cggcgctcgc cgcgctggcg gcgggcgacg cgcgcagcta ccacgacatc
     6901 ctcgggccca cggtgccgct gtcgcgccac gtgttccgcg cgccgacgcg ctactacaag
     6961 accggcgtgg tgttcctcgc ctggctgaac ggccaccagg agcacttcgt gatggtgggc
     7021 ggccagcaga gcgcgcgcca catcctgcat ttcgcggagc tgttccggct ggcggacgcg
     7081 gccgggctgc tcgccgaccc gccgctcgcc tgcgcgcgca tgaagcagct gctcgccctg
     7141 cacggctgcg gctgagcgta cacttgacca cgttccgcat cctaggagga gacaccatgc
     7201 aacgcagact gttcctggcg gccgtcgccg ccgccttcgc gctgcccgtg ggcgcgcaga
     7261 ccatcaccct gcacggcgcc tcgcagttca gcgacgagca cgccttcacc aagtcgatgg
     7321 tgaggttcga ggagctggtg aagaagtact acggcaagcc ggtgaacttc gtgctgcaca
     7381 agaattccga gctgggcctg gagaagcagt acttcgagta catggcgcag ggcaaggcgg
     7441 tggactacgc catcgtgtcg ccggcgcaca tgtccacctt ctccaaggcg gcgccgttca
     7501 tcgacgcgcc gttcctgttc cgcgacctca agcactggaa caaggtgctc gacgccgaca
     7561 tcctcaagcc ggtcgccgac gagatcgcgc agaaggccga cgtgatgctg gtcggctacg
     7621 ccggcggcgg cacgcgcaac atcttcgtca acaagccggt gcgcaacatg gccgagctca
     7681 aggcgctgaa agtccgcgtg cagggcgcgc cgatctggtc caagaccttc caggccgccg
     7741 gcatggcgcc cacggtgatc gcctacaacg aggtgtacaa cgcgatccag aacggcgtca
     7801 tcgccgctgg cgagaacgag gccgccggcg tggagcagat gaagttctac gaggtggcgc
     7861 cgcacctttc gatgacgcag cacgcgatca ccatccgccc gctgtgcttt tccggcaaga
     7921 ccttcaagaa gctggacaag gatcttcag
//
